LOCUS       DAOMZL010000001        92964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_120_length_92964_cov_14.853696, whole genome shotgun sequence.
ACCESSION   DAOMZL010000001 DAOMZL010000000
VERSION     DAOMZL010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 92964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 92964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92964
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120_length_92964_cov_14.853696"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="PLQ30_00005"
     CDS             complement(<1..1102)
                     /locus_tag="PLQ30_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW00856.1"
                     /translation="MDEYFDAEPVTKGYDAQIARRILLYLKPYGVYAIIAILALAIAT
                     TGELISPTIIRRAIDDSVVRDWYGLDPSALSMLPGNDSSHEVEIGGRIYVRSSNLAGL
                     TMAERDHLREQGLLDNEASYVFPLDPMRDKKLSVMDGIPKAELSDKWGVIPSSVLRTL
                     KSEDAAILRAADDKSLLRFGFILLVVLLIVLGATFAMTWFTNQISTLIMKDMRLELFH
                     HVMEQSLSYLSRQPVGRLVTRLTSDIEVISQFFSDVLSAFIKDASIMIGSLIVLFLLS
                     WKLALIVLTTMPFVFIAAAIARIKARDAFRNQRYWTSKVNSYLSEHISGIDIVKLFVQ
                     EDKVSENFSKNSKQLLKANIAEMYVYATFRPFV"
     gene            complement(1095..2936)
                     /locus_tag="PLQ30_00010"
     CDS             complement(1095..2936)
                     /locus_tag="PLQ30_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW00857.1"
                     /translation="MLKEYATLLPHLRSYRYRYVAGILCLIVVDASQVLIPRYIQIAI
                     DRISSGAFALVDIIDPLVHMLLLALLISIGRFFWRYFINIASRRIEAEMRDRLFSHIL
                     SMSGNFFRENTTGDLMARATNDISTIRQATGMGFVSLIDGIFMTAMIIAAMFLNNSAV
                     AAWIILPLPLITALILLFGRIVGRLFKKIQDIYGYLSNIAQESLSGIRIVKSFVKEDY
                     FYNRFSMANTDYKNAIMDLVKTSGFFFPFITFLSGLSTILLIIFGGRASIRNKMTPGS
                     IIAMLSYLEMLVWPLMSAGFTVNIVQRGAASLKRINQILRTAPEIKESSNCIRKTPRG
                     DIDIKRLYYIYPGSQKPALKDISVHIPEGSMLGILGKVGSGKSTLLKILPRMLEPGEG
                     HVFIGNIDIWSYGLRELRSAFGMVPQESFLFSDTIEHNILFSAHNISPERFKAITKIS
                     GIDHDYQLFPNGWETVVGERGITLSGGQKQRIALARALAADPNILLLDDALSAVDAQT
                     EEHILSSLIDERKAKTTIIVSHRISTLRNADYIIVLDSGEIIQQGSHEELMAESEGFY
                     ARIAALQQLEQQVSIDSIKNNISQRETLSREKPLGQDTSVSEEKIYG"
     gene            3013..3522
                     /gene="rnhA"
                     /locus_tag="PLQ30_00015"
     CDS             3013..3522
                     /gene="rnhA"
                     /locus_tag="PLQ30_00015"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HPW00858.1"
                     /translation="MNQQEFLVYTDGGCKGNPGPGGWAYVMHYGDRYREAWGGELYTT
                     NNRMELIAVIKALSFLKSRIENASSSVSEESLEAPSWTRAKINIFTDSMYVKNGITEW
                     LIEWKKRGWKTSAKKPVKNLELWQELDSLCCELKPHFEWIEGHSGNIDNERCDRLVHL
                     AIEDVLCQK"
     gene            complement(3679..5136)
                     /locus_tag="PLQ30_00020"
     CDS             complement(3679..5136)
                     /locus_tag="PLQ30_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW00859.1"
                     /translation="MRKSIIICLVVLFGLVTSCASVPSIPEWVKKTPSPDAKYTYFVG
                     SSSAKDSATALNDATAALIAGIMQYMGVSISVASSAEARASLSEYEAQITQTVKTEAK
                     GRLSGFEVVEKFIEQDKKSGLYTVHVLARYETKELNKEKARMESLLKEKADAVAIPEQ
                     KGDAAANEGLLIDAIRAYAEAMSAAASSEVENAQIKLARNAKKASELAATLRLAILSP
                     SNIEIQLGGAKVPFITVKLIANRGGREEGVPGAPIAITYPRRLTSGRIGTATQQVFTN
                     QDGIVSFDVPPIELAGKYQVTAQLDFGSIADILGGLPKWALPYIDALESELSGIRASM
                     SYKVISAAKNVPTAIGVYLKSEAQKGSLDPEPFSKSLEEALVNEGFSIFTTKISAESG
                     QPDIDRLRAIAPAEAMRFVLACVELISIGKEDGFFIASVAGSLSAIDLIEGNTLYSGS
                     KGAQGMGLSEAEAISSALRTLGGQVFAKELLAALR"
     gene            complement(5241..5894)
                     /locus_tag="PLQ30_00025"
     CDS             complement(5241..5894)
                     /locus_tag="PLQ30_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW00860.1"
                     /translation="MKARKAVLVLLVAAVIISCGTKPPLRKGEVLIEDKGTAYGIKTP
                     KWVELAIIGGYRDIEKLPDYKDKVVFIAQFEAQNLQSAQLLAERMQADTEIARYLSTR
                     VKDAFKGANVADADSKNFGAYGERFVMSVGEATFSGFRMEADWWVKVQTYTPENKPDK
                     QIYRVIQLWAIDKNMLQKQFDMLFSQMAGGEPPTPEQKRAMDLVQNTVTKDFFGESD"
     gene            complement(5933..6985)
                     /locus_tag="PLQ30_00030"
     CDS             complement(5933..6985)
                     /locus_tag="PLQ30_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW00861.1"
                     /translation="MGVLLLIGLLIGCATTSQPEAPSLESQRAKEIVQGTSTPEMPGT
                     TAPGDVQSSAEALVESQVMPGETPAPSEPSSVPQASASYYTQPMTADESRFFQNYINR
                     LSYLVYYNENSGLDAQLAKAAVSQANRYLIEKEGLSVIDFDQIQKNKKDQISAYQSET
                     GGSIDIITYIAQKLNADIYLEIDAKTSFGGGPGAWTGSAQGSIKIFDASTAALLGSIS
                     FLSPQTFSPVSADAAMMNAISGIIWQSMPKVTEQAKHLMSTMTASRGVRYELILQNTP
                     DAIAISQFERNLAKRVREVERLSYSPGETRFAIYAFMPASKIQDAIYDAASMSGFPNC
                     YLLYMRGRSYTFNTGI"
     gene            complement(7097..7170)
                     /locus_tag="PLQ30_00035"
     tRNA            complement(7097..7170)
                     /locus_tag="PLQ30_00035"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7134..7136),aa:Arg,seq:ccg)
     gene            7252..8007
                     /locus_tag="PLQ30_00040"
     CDS             7252..8007
                     /locus_tag="PLQ30_00040"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPW00862.1"
                     /translation="MKEHIRKSIKVLSKHPSLIRLLFVMYGEPILLASESSRRASILE
                     SIRIPFIKVLPSIDETIFDNLDPAERVVALAATKAKHGKALWSEKNRSKKVVISSSAK
                     KPLDKNALDQKSNAEPRYVLGADTLVAFKISGRWITLGKPANELEAFEMLSMEAGKRQ
                     TVFSGLCLLDMVSDRCYPALSISEVQFAPMTPEEVSWYLSFGEWRGAAGAYRIQEIGS
                     CFIEEIRGSSSGVMGLPIHELYGILHASGYLDS"
     gene            8138..9019
                     /gene="rpsB"
                     /locus_tag="PLQ30_00045"
     CDS             8138..9019
                     /gene="rpsB"
                     /locus_tag="PLQ30_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPW00863.1"
                     /translation="MAVVTMKNLLESGVHFGHQVKRWDPRMKKYIFAERNGIHIIDLQ
                     KTIQAIKEAYDAVQKVASSGKSVLFVGTKKQAQAAIQKEAERCEQFYVNNRWLGGMLT
                     NFSTIRKSVQRLKKIERMEVDGTFSSLTKKEIASLLKEKTKLEKNLGGIKEMTSLPGI
                     LFVVDTRKEAIAVAEAQRLGIPIVAIVDTNCNPEGITYPIPGNDDAIRSISLFTQIIA
                     NAVIEAGASEGLKIIETLPSEEEQELIVPQKLEEDEVEIDVENYAETPSQSNKEADEQ
                     QEVEQPIIDEDRLYDED"
     gene            9041..9892
                     /gene="tsf"
                     /locus_tag="PLQ30_00050"
     CDS             9041..9892
                     /gene="tsf"
                     /locus_tag="PLQ30_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPW00864.1"
                     /translation="MEIKANEVKALREKTGAGMMDCKKALIEANGDFSAAEKLLREWG
                     IAGIEKRAGRATNEGRIFIAQNDKELELIELACETDFVARNEDFVAAGTRIAESALSQ
                     KASSTNESLEAIVKEIASLIKENIALRRVVHFYAEENEILHPYLHGEGRIGVVVKVRA
                     NDPEAFKHEKIAAFIHDIALHVAAFNPMFLDESKVSAEWITEQKEIFQKQLELDEKLK
                     SKPEKVLEGILAGKLKKLLADVCLLNQGFVRDEKSSVASILAQISKETGFQLEIVDYY
                     FAKVGQD"
     gene            9980..10534
                     /gene="frr"
                     /locus_tag="PLQ30_00055"
     CDS             9980..10534
                     /gene="frr"
                     /locus_tag="PLQ30_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPW00865.1"
                     /translation="MESNKAAHEEKMKKTIAAFQDELNSIRTSRASPVLLDKIRVDYY
                     GQKTPLSQVATISVPEARLIVIQPWDRSLFSEIEKAILKSDLGLNPSNDGKVLRIAIP
                     PLTEQRRKELVKTARSIAEQARVAIRNIRRDALEELKKQQGAGGVAEDVLKKEEAELQ
                     KMTDDYISQIARILEIKEREIMEI"
     gene            10547..11251
                     /gene="uppS"
                     /locus_tag="PLQ30_00060"
     CDS             10547..11251
                     /gene="uppS"
                     /locus_tag="PLQ30_00060"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HPW00866.1"
                     /translation="MKENRMPSHVAIIMDGNGRWARQRGLARTEGHKEGLKTAKRIVL
                     ASRKEKIEFLSLFVFSTENWKRTEQEVGFLMSLIRAHITKELNFYRKNEIRIVHSGER
                     NGLPHDVLSEIDKAIENTSQYSSLTVNLAINYGGRDEIVRAMQRILASQENDDENPID
                     ELKIMRYLDHPEIPDPDLIIRTGGEIRLSNFLLWQSAYSELYFSPKYWPDFTPEDLHA
                     ALIDYVHRERRFGDAR"
     gene            11248..12126
                     /locus_tag="PLQ30_00065"
     CDS             11248..12126
                     /locus_tag="PLQ30_00065"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPW00867.1"
                     /translation="MNENMRARLLLFFVGFPLLILSLFFLQFANYIVISGILLIVQFL
                     SSKELRNMLTRASLPDTGDLATFIGLIPSVLTFISCILYGSLLETAFVLFISWSFCLM
                     LLLIPLVFQKKNSFPDLIKQAASIALVHCYTALLPGFLIFIVSGFPNSQNTIISFALL
                     TFGNDSLAWFFGKYLARKRNLLDVSPNKSISGFIGGTIGSIVAAFIGLGPIAGAWKPS
                     GIAYILLSLSLGLGMAFFVIVGDLFESALKRSAGIKDSGSIVPGRGGILDSFDSLYFS
                     APFFIAFSYTFHLFNL"
     gene            12163..13290
                     /gene="dxr"
                     /locus_tag="PLQ30_00070"
     CDS             12163..13290
                     /gene="dxr"
                     /locus_tag="PLQ30_00070"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPW00868.1"
                     /translation="MVLGASGSIGRQALDVIQRSADSCNKLELVGFSIHSNRILAKKL
                     KRQFPQAKGAWTGNPSLAPEEMDWNGLDAISNLLLETEAEIVLNGIAGAAGLSASILA
                     LQNKRDLALANKESIVMGYRLLRNLAQKNQCQIIPVDSEHAGIFQIVQRFGRDLVSEL
                     IITASGGPFYALPLELLDAVTADEACKHPVWQMGRKISIDSATMANKGLELIEASRLF
                     DMPQERIKVLIHPQSYVHAMVRTIEGVLYAQISKPDMRLPIQMALYWPEIVETSFGFA
                     DLADISLDFYEPKQERYPMLWIARQAIDEGDAPCIVFNAANEIAVECFDNGRIRFTDI
                     SNLVAKVLDRDWNLPIESFEDIFDIDIKARRIAAHLSESCG"
     gene            13287..14639
                     /locus_tag="PLQ30_00075"
     CDS             13287..14639
                     /locus_tag="PLQ30_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPW00869.1"
                     /translation="MISIILGLIGLSIVVIFHEFGHFVFARLSGVEVEAFSVGWGPSI
                     ISHKGKKTVWKLCAFPIGGYCKLKGEEGFQAAVDQNLQYIPSEKGSFFSATPLKRIFI
                     ASAGPLFSVLFAAFIFILVASIGTTIVTAPNRIILSSEIGSTLQSGMPNPADIAGLKT
                     GDKIISIDGKTIRDYSDLQEIIASNPGKTMSIEVLRNDKKHSLVITPLLDPNSGIGVI
                     GVYAWIDPIIESVQEKSPAAIADLRPGDIIMEADGRPISNTVDLMQVFNNANGSVSLR
                     VKRGDQTLSMIIVAKSLEEAGIRFEGILRTEKAKSLPEAISMGVEETFSTISLEIKSI
                     GMLFRGVNVFKAISGPARLTYLIGTAASEQIKAEGLAGLVPILDFLAFISIALAIMNL
                     LPIPALDGGRVLIYLIELIRRRPINAKSLYRFQFIGVASILLLFLVATMSDIFFFLGK
                     "
     gene            complement(14658..16001)
                     /gene="dnaB"
                     /locus_tag="PLQ30_00080"
     CDS             complement(14658..16001)
                     /gene="dnaB"
                     /locus_tag="PLQ30_00080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPW00870.1"
                     /translation="MAGPSLKDTLPLYNGDAEQACLGALLLDPESINNVIRYLRPDSF
                     YDSANQEVFEALLAMHEKGQKPDLITLSEELRSSGNLERIGGSAYIASLASFTPSAAN
                     IEYYAKIVQEMATRRRLVHLSAETAAMAHEETKDIDAILDEIQAKIFEISQNRRTAEY
                     HSAKEIVTETMLLIEKLSTDPAAFTGIPSGLSDLDALTNGLQKSEFIVIGARPSVGKT
                     ALALTMAAHASINLKIPVAFFSLEMSESAIMLRLIASEARIPADRIRTGRIKTTDYDS
                     LMDAASRIYEAPLYIVDLPNMKLLELRTMARRLVLERGVRAIFVDYLTLITHENADLP
                     RWEQIAAISRSLKALARELDIPIVALSQLKREAEGKQPTLADLRESGSIEQDADLIIF
                     LHRDRETSKTYDSQTDQIETDIIVAKQRNGPTGKAPVWFKSSYAKFVNMERRDRT"
     gene            complement(16052..16645)
                     /gene="rplI"
                     /locus_tag="PLQ30_00085"
     CDS             complement(16052..16645)
                     /gene="rplI"
                     /locus_tag="PLQ30_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPW00871.1"
                     /translation="MKVILNQDIPNLGEIGDVKEVAPGYARNYLLPKKMVMVYNEKTE
                     ALFLKRQAEIQTIKEQKRLSSRTLKERIEAEPLVISMPAGKNGKLYGAVTSHTIAEEL
                     LKKNIEVDRKRIEVPDRSIKSVGNYKIVIKLYEKDEAILHCNIEAQASASAEEKKPSE
                     EAGKKRRTRRQRTESEPATPEEQAAAFEAAVNRGQLS"
     gene            complement(16648..17622)
                     /locus_tag="PLQ30_00090"
     CDS             complement(16648..17622)
                     /locus_tag="PLQ30_00090"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HPW00872.1"
                     /translation="MEVSQSIKSESSSKYSWRVFIPYGLLSGFLYASGFLAFAFLIPI
                     QYVFRKENKREGLLSILVSLSIVAAISTIELSRMGWKELPIFLQTLLLPALLLATIGA
                     MNLLKSDAWVKIIAATIVVSVLFAYLFSISIGTPEARRSIADMIAQLLNSSGIQDLDA
                     SAIEQNYVIPAISIIMRSFGVASWLLLAGSWHIGNSLASARIWKQKGEAKKNAFVFSV
                     PSWLLWPSILGWLALICVLYGNERGVFAIIAWNVSLAAACWYALQGFSLISYFFRLRN
                     MQRLAGLLLILLMILMFYEAKIRIAAAILAPMLGVSEVWLQYRMHKGA"
     gene            complement(17663..17971)
                     /gene="rpsR"
                     /locus_tag="PLQ30_00095"
     CDS             complement(17663..17971)
                     /gene="rpsR"
                     /locus_tag="PLQ30_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPW00873.1"
                     /translation="MRDIDESRASNVEAEQQELHRDQDDRNGVRKGKGFFRKKVCRFC
                     TQNVKIDYKDAELLRRYTTERGKILPRRITGTCSKHQRMLAVNIKRARALGLLPYVIK
                     "
     gene            complement(17993..18448)
                     /gene="ssb"
                     /locus_tag="PLQ30_00100"
     CDS             complement(17993..18448)
                     /gene="ssb"
                     /locus_tag="PLQ30_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPW00874.1"
                     /translation="MSDINVVVLVGRLTRDSELKYTPSGLPICRFSIAVNRSKKQDDQ
                     WIDEPHYFDIEFYGKSAEGLSKYLFKGRQVAVQGELRQDRWEKDGQQRSKVVIVAGTI
                     RPLGAPSQNSGEMGGRRYAADNIPSKIDSANNQMPIPPTIDEFTDDIPF"
     gene            complement(18482..18763)
                     /gene="rpsF"
                     /locus_tag="PLQ30_00105"
     CDS             complement(18482..18763)
                     /gene="rpsF"
                     /locus_tag="PLQ30_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPW00875.1"
                     /translation="MRQYELVTVLSSEEEEFKAGKQAIADMLSQYKAIDVKEEDMGDK
                     PLAYPIKKKTRAHYMLYRMSFDPASIVSLERAIKLNPLVLKHLVVKVEE"
     gene            18999..20330
                     /gene="hisS"
                     /locus_tag="PLQ30_00110"
     CDS             18999..20330
                     /gene="hisS"
                     /locus_tag="PLQ30_00110"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPW00876.1"
                     /translation="MTVIKPRVLKGFRDFLPDAEITRSSLVRELEQVFALFGFVPIDT
                     PVLEYADILLGKGGGETDKQVYRFLDHGDRDVAMRFDLTVPFARFMAEHVDELYLPFR
                     RYHIAKVWRGENTQRGRYREFMQCDFDIVGTDSGSADADILLTAAKAMLRLDVGEFSI
                     RINHRALFNRFLEHIGASARSTEILRTVDKLEKIGFENAFEKLSKLLGMQLADDILSF
                     IKKEESFEATLEKMIGFCDKNDFQAQSARDRLLEIMEYVAAAGFENRIMLDPSITRGL
                     DYYTGMVVETSLGALPEIGSVCSGGRYDELASLYTERRMPGVGASIGLDRLIAALETI
                     GKIDKSIISTQILIMNQEKGNVGKVATSELHALAARLRDAGFSCEVFPEEKKTLAQYT
                     YAERKHIPLALFTNSAKGPYNIRILAERRNIECLDFDSLCKFIRSFLNVQT"
     gene            20317..21060
                     /gene="trmB"
                     /locus_tag="PLQ30_00115"
     CDS             20317..21060
                     /gene="trmB"
                     /locus_tag="PLQ30_00115"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPW00877.1"
                     /translation="MSKPEMEEGQDRKPEQTQSMSQEIGLLDGSIRSFVKRSGRITEA
                     QRRAIKLLGPKYIIPFSEELLILEELFPQNRPIILEIGFGSGQATWRIAQERPEFNYL
                     GIEVYASGVGRLIMDLELHAIENVRILQQDAIAVLRHMIAPRSLAGIHVFYPDPWPKK
                     RHHKRRLMQYPIVFLMAQKLMPGGYLYCVTDIEEYASFAKASLDACSLLENQYEGFAP
                     FQPWRPETRFENLAKEAGNQHFELFYRKN"
     gene            complement(21180..23138)
                     /locus_tag="PLQ30_00120"
                     /pseudo
     CDS             complement(21180..23138)
                     /locus_tag="PLQ30_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690073.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA];
                     GO_process: GO:0008380 - RNA splicing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase"
     gene            23223..24476
                     /gene="xseA"
                     /locus_tag="PLQ30_00125"
     CDS             23223..24476
                     /gene="xseA"
                     /locus_tag="PLQ30_00125"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPW00878.1"
                     /translation="MNFDSNLDAESRLFSPLSVSQITSLIRNNLENKFPDVIIEGEVS
                     NCKMAASGHLYFSLKDEEAILQVVMFRRDLLTLDFVPKDGMQVWARGAISVYAQRGQY
                     QLIARNVRQTGFGDILAMLEQRRRKFAEEGLFDESRKKEIPAFPTRLGVVTSPTGAAI
                     RDIIRVIQRRNPKVSILILPTLVQGQEAAASIAERIRQANLWNLADVLIVGRGGGSIE
                     DLLPFSEEVVIHAIVESNIPIISAVGHETDWALSDYAADLRAPTPSAAAEMACADLYS
                     IYKEIAHFENIMGQSMKHSITNAKQRLSSISPHIMEDILLRKNLVASQRFDSAIESIR
                     NSFQKKAEDLNHRLVLASSAIEIANPREIMKRGFSVVKKNGVDIVHSSRMLALGDSIN
                     IMFYEGEADADVKATRDYGDKRSSG"
     gene            24473..24754
                     /gene="xseB"
                     /locus_tag="PLQ30_00130"
     CDS             24473..24754
                     /gene="xseB"
                     /locus_tag="PLQ30_00130"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPW00879.1"
                     /translation="MKDFEKRIAHLEELAEKMRDPDLPLEKSFGFFEEGVMLARELRK
                     ELDELQGKVEILLNSLEVRENDEAEVPNITKFDFQSSESQNDSQDKQAP"
     gene            complement(24761..25198)
                     /locus_tag="PLQ30_00135"
     CDS             complement(24761..25198)
                     /locus_tag="PLQ30_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPW00880.1"
                     /translation="MVGQDDTAGKIESLRSVAGIAIKSGLLFVARRKKDHTEMSHRWE
                     FPGGKVEEGESDESALKREFLEEFGANIDVIRFLGESIFENKGRTRTLAAWEVSFSPG
                     SIRQLNEHVEVAWLPIESISNMELADSDRSLIPILEAAVKPGS"
     gene            complement(25214..26887)
                     /locus_tag="PLQ30_00140"
     CDS             complement(25214..26887)
                     /locus_tag="PLQ30_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPW00881.1"
                     /translation="MHVLISNSGSIELDALTRSEFNFSVDHLMEIAAIRLWQTLLSEV
                     IVKKNMSSLISAGSSSSRQPGIAAVCGKGDNAGDALAMLRHARLEGYSRLTAYIPEPN
                     TMKNEVISNLRRAERCGTEIICYETIDLKSIGERLSTEDIILDALFGTGIEGPARGRA
                     SDLIQILRDVRIARNERCKKNPLIVSVDVPSGLSDGWRPEFPIVCADITLCIEPMKES
                     LYMPKARPYAGDIIPVGSIFPLWTKASTQKTWLLEEGDMKGFLPPISPWAYKMQRGRV
                     AVFAGSASGAGAALHCVRGAASAGAGYIALFCDEELFAAYLAAIGESAIVRVFSEDSF
                     SSECWDVVIAGPGWGIDPGRERILDMLLQAHIPLVLDADGARLFARMARANQDSSFFG
                     GPIILTPHPGEFSEFIPMLGSDFSGKTSETVASVSALAKKYEIALALRASTTHVGLPD
                     GTCFIYDGSTPGLGIAGSGDVLSGMIGGFLARWIAFSKERKENRESTEPATSSDNPVA
                     RALLGAILVHGLAGKQLWLKKGWFTPLDLANACARISSGAMETDMDNDR"
     gene            27092..28402
                     /locus_tag="PLQ30_00145"
     CDS             27092..28402
                     /locus_tag="PLQ30_00145"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW00882.1"
                     /translation="MSLGINTSLIQALEILGINEPTEVQKQAVPLLLEKKDLSMQSET
                     GTGKTLAYLLPIYNTILSNIDEADRRWPVAVIICPTQELAVQVANHSAQLAMALQSKN
                     NGAMSQSDSCQQQGLSKLRNRAPKIDSNLLNSPQVRSLVLLGGTQFTRQKQALKIHPH
                     IIVGTPGRLADLTRLRYFDLSHLQFLILDEADRLFSREYIEPVEFLLSNAPKNCTHVM
                     ASATIPERTRNRAKPWIPKPIVIELEREGVLSQAIEHWAFYSDHRKKIDLLKRIIVAI
                     HPKRCLIFASEAYRVQRAAERLNSSGLQCASLVSRMEKQSRHSIIEQFKTGGISLLIT
                     TDLAARGLDIPDITHVISLDLPEDGGVYVHRAGRTARAGKKGISILIADKLELERASR
                     TATRFGFVFRTKWLEYGTVMEPPVEAFFERVEDMETQKRSKSHL"
     gene            28399..30951
                     /locus_tag="PLQ30_00150"
     CDS             28399..30951
                     /locus_tag="PLQ30_00150"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW00883.1"
                     /translation="MSASTISEPKIVTEAVAADIANPRFEDAQYRILIVRLSPFRDVE
                     GSYTHQVLFDEARHALPKAYIDFAFMPSFSHRKALSIAKRPWFFGRASNRSATEFNML
                     LISCAFSLELLNIPWLLSQSGIPFSRHERLERYDLPFLFLGGSSAVCSGSLVKISDNK
                     VINSLVDAIFFGEGEGRISEIVRIASDDSKTGLSKLSILAHIASEVEGFWPCDSSFTC
                     NRALSFQRPAVLTSPIVLNSENADTVKLAISAGCIGHCTFCLEGWDRRPYSEKPSDYL
                     SKSAVVLKRASGASDVELFSYNFNMHKDIIQLIQILGRYFMKVSMMSQRIDILSNKPE
                     ILAAELAAGKRSFALGIEGISERIRKYYQKGISEGQIWNSITRIIDNKAREIKLFFII
                     SGFENGHDLEEFENFCDKLAHYKIETQSVTRVIVSAGYLVRLPFTPLQYAPLQGDRAL
                     LQSIASALRISCNKSNLEFRLASSFEDYWMDQLLSLGGSRMHDWLQACPENGFLYDLH
                     VSDRALNSLCAFFNRESCFTQLLQEKHHTYRPDFDFLESARHWQMLAELYDRSIDHLC
                     STDSKKFYTKMPAIPLISLEAERTIDIIKAQQKAKAHFPSILVRIRENDALAFSTPAY
                     ERAWLLRTLSFLVPNAERALFYCKLQLPNSDWESAIPTKSASMPCKLRIGISGVKYFA
                     IYGPDKTDMERIINLAISKIKHSNETKKIGLSSISSNYSGSTAFLEEIEIIQELPQAE
                     TCRLSFCIPANRDGFLNEVLEGWLNELNLHFTLQKDEDSIIYQTYSAHKANKSVQYIQ
                     YKTSYTNISLCLSIGKKADIALLADFLYDKKCTLEETIFRIEGWDLSNVCLA"
     gene            complement(30953..31336)
                     /gene="queD"
                     /locus_tag="PLQ30_00155"
     CDS             complement(30953..31336)
                     /gene="queD"
                     /locus_tag="PLQ30_00155"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HPW00884.1"
                     /translation="MYHIRVETEFSAAHRLFHYNGKCERFHGHNYRVRVWASGKELGE
                     GGMLIDFGILKSALKKLIDEEFDHQDLNQIAELAEDPSAERIATLIYSRLHRDFSSLP
                     ISAVDVFETSSSMARFVPDAWPDAR"
     gene            complement(31373..31498)
                     /locus_tag="PLQ30_00160"
     CDS             complement(31373..31498)
                     /locus_tag="PLQ30_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW00885.1"
                     /translation="MNLNNLKASIDYERTDKMFFEKSYIIWRLESPVSAIMNRVF"
     gene            31538..33226
                     /locus_tag="PLQ30_00165"
     CDS             31538..33226
                     /locus_tag="PLQ30_00165"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPW00886.1"
                     /translation="MQKRLSDLIQGLDICSIEGDIHHLISGLFYDSRECKPGSLFFAL
                     PGLHTDGKKYIIQAIQNGAVAVIYEGLLEKNLREFMLKDPSARALNPALIRVTDCRWT
                     MSAISASFFENPSDSLCVIGVTGTEGKSTTVSFIYQLLNAAGFKAGFFSTVMSDIGEG
                     EQPNPQHQTTPEATTVQQMLAEIRNHGLQFAVIEASSHGLSPRTARLAHVHFDIGLVT
                     NVTHEHLEFHGSWEQYRSDKANLFRRLGEVEPKKLNKVSHAINEHNSNIIISPTGIIC
                     ADDPNASYFMHESAVPCLTYSSKGALADLSAHNIIEDANGVSFEIEGLLEMPIGHNFR
                     TEQEISARRFENVMPSTLSARINLPGTFNVQNALGAILAASAATGLHWHNFVPLLPGL
                     RPIKGRMQRILEGQPFEVIIDYAHTPSSFKEILPSLRKERRGRIICVFGSAGERDTEK
                     RPQQGRIAADYCDILILADEDPRGEDPMAILEEIAMGCPELSREDRLYLIPDRPEAIR
                     RAFNLAKPGDLVLLLGKGHENSIIYSDRTIPYDEERQARSILADLGFEITNKGK"
     gene            33230..34348
                     /locus_tag="PLQ30_00170"
     CDS             33230..34348
                     /locus_tag="PLQ30_00170"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HPW00887.1"
                     /translation="MKSIAVLYGGRSGEHEVSLISASSILAYLDKDKYSIVPIGITKR
                     GEWFLQHPPEWAYSRPENGQMRSLPPVERSERVLAVPGEGLWLETTTKGLQKLNIDVV
                     FPVLHGTFGEDGTIQGLLETVDLPYVGADVLGSAVGMDKEVSKRLWLSAGLPVVDYIS
                     VRKEDVSDENLKILARKVETRFGWPCFVKPACSGSSVGTSKVTHSEELKDAILSALRW
                     SEHALIEAFINAREIECAVLGNERPMVFPPGEIVPSHEFYDYEAKYKDPQGATLLIPA
                     PLLEETKNRIMSLALSAYKYASVKGMARVDFFVEKITGEVFLNEINTIPGFTAISMYP
                     RMCIDAGVSYSELLDKLLELAIEQYAIKRELEYDFSKA"
     gene            34547..35746
                     /gene="rocD"
                     /locus_tag="PLQ30_00175"
     CDS             34547..35746
                     /gene="rocD"
                     /locus_tag="PLQ30_00175"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HPW00888.1"
                     /translation="MNSQDYIALDYQYGAHNYHPIPVVISMAKGCKVWDPEGREYYDF
                     LSAYSALNQGHLHPHIVNAVKSQIERVTLTSRAFHNDKMGPFLKKLCEYTGYEMALPM
                     NSGVEAVETAIKAARRWGVEKRGIENGRQSIICANGNFHGRTLSAVSMSSDPDSYINY
                     GPYVPGFIKIPFGDAKAVSDAIDENTVAVLVEPIQGEAGVVVPPDGYLSDLRRICTEA
                     GILFILDEIQTGFCRTGRHFAWNYENARPDIMCLGKALGGGIIPISAIVADHEVMDVF
                     TPGTHGSTFGGNPLACAVGIAALEVLEQENLDERAFRLGERFREVIKKAKSPHFRLVR
                     GKGLLNAVVFEEGFDAYPTCIDLKESGILAKQTHGNIIRFAPPLVITEDELEEALEKI
                     IYVLDRA"
     gene            35767..37197
                     /locus_tag="PLQ30_00180"
     CDS             35767..37197
                     /locus_tag="PLQ30_00180"
                     /inference="COORDINATES: protein motif:HMM:NF037483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW00889.1"
                     /translation="MNNFKSLVSHLYFVAFFAAFLIAVYLIVIQRQETLQASVPIENR
                     TIVLESLSDKSQVSPQNNSLQHAKISPLPGDIFLKTIDIDVDDDEDFEQVILCKKTAG
                     STVLWMIIADFSPNLGAYLRHFEAEIRASKLESITVQPLDVTGDGLSDLLIQGLDSSN
                     NQTLTIFRRLLNRGYSLIFSETGLSINLQDSDESAIPSIVVESLPNKTGLVSYKTYIW
                     NRNSLFLEKVSESPLRESNSISEGPSGSDAQSYLSWLNRFWKKNSDASNICSLYLDTN
                     SNALILEGQQIQQRWIIKSAERKGNRLYLTCRLSELSYLDRIFIIDAKGQNVISVSII
                     DQQVSHFRRDEGWSGKYESYDYMKEAKDNKTILSSPDHSQFLGRYLGDDHSVLVLDQS
                     KNILVLNGKYREGAARIYYYDGYQVLDFLQIQSNGLVGDRLLFIVSIDQSENGKINRL
                     ILRPAEIGADGVRMEYRTPYIFIKTS"
     gene            complement(37194..38228)
                     /locus_tag="PLQ30_00185"
     CDS             complement(37194..38228)
                     /locus_tag="PLQ30_00185"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPW00890.1"
                     /translation="MKRNRIGITRDRSSLNFAASFVLFLSLFILIINSSIEAETYKSL
                     SQARQAASKTKSQDALRDVLLSAVSQLSVRDGIALCQEYEAKTDQNLKAELRGILGGL
                     YLLLGQTKEAASWYIKAASIDAKYTNEALRLSIAIGDQKNALALLKNESISPEFKHIF
                     DIWLSLYDGEYASASSKARDALAIVTKPQLRRELLLLQYFADFGEFGAGISSLVKDFP
                     NSIESDLIIGRVFPSSSLMLFLGFSWIKGPAPLADYGVTSNNSQIAEEHSAASGIQWL
                     QAGYFSSKDNAERLSRNLTNQKFKTRVIELKNQAGEPRWAVHVAAEEDWKKTQSKLKD
                     LGYESYPAGN"
     gene            complement(38240..39607)
                     /gene="miaB"
                     /locus_tag="PLQ30_00190"
     CDS             complement(38240..39607)
                     /gene="miaB"
                     /locus_tag="PLQ30_00190"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPW00891.1"
                     /translation="MNNYHIETYGCEMNKAESAAMEATLREHGWKQSEEREAQLILLN
                     TCTVRATAENRVWNRIHQLTARKKEHDFVLVVIGCMAEQYKNAIKKKAPGVDYIIGTY
                     QRQSFSLMLDSIANGQKIEVLEENPIYIFSEDHHETGAFRSFIPIIHGCNNFCTYCIV
                     PYVRGREISRSPLDILREIDRLEDLGVREIILLGQNVNSYTWEKDGASLDFPGLIKMI
                     AAHLRKKQESEGTIECYNASPKKSKRGIGWIRFITSHPKDLSKELMAVMAEDPFFCHH
                     LHLPLQSGSNTILSAMNRKYTREYYLSVIDALRNAIPDLTLSTDIMVGFPGETEEDFE
                     QTLDLMRSVRFSYAFMYHFNERNGTFAAGMPNKITEKIKKERLARVIALQKEITSSLM
                     RERIGHVDEVLIEDRSKRSTRELLARTSRDEMVVFPGSATRIGQFAQVRFISVVGNTL
                     RGEEV"
     gene            complement(39604..39960)
                     /locus_tag="PLQ30_00195"
     CDS             complement(39604..39960)
                     /locus_tag="PLQ30_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW00892.1"
                     /translation="MESNYPVDPKTLNDKGTKGVMSSVAGLGFLGVEALLGIPVVGTV
                     ISAGLIGLGAIGLLGKTKTDKISGTVLGVAGIAGLTTLFLPGVAHSLLSLGGIGLLGY
                     GLFNLAGFIRGLKKKK"
     gene            complement(40030..40866)
                     /gene="folE2"
                     /locus_tag="PLQ30_00200"
     CDS             complement(40030..40866)
                     /gene="folE2"
                     /locus_tag="PLQ30_00200"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HPW00893.1"
                     /translation="MVDVQSFRDDRNVAIQKVGVKGLRYPITLLDKAEKYQHTTAAVN
                     LFADLPHDFKGTHMSRFIEVFEEYRTDLSMPNVIKMLRKLRAELDAQTTYTDIHFPYF
                     IKKKAPVSEQASIMNYECFYEASCSGKGHRFIAGVIVPVQTVCPCSKAISDGGAHNQR
                     GIVTLQVSLGPFFWFEDLIRIIEESASSELFTLLKREDEKYITEKAYMQPRFVEDVVR
                     EVYLKVDALQRFPWFSVEAENFESIHNHSAYAYVEKSDRSKIEPVSGEHGRLDRLESY
                     YP"
     gene            complement(40948..41832)
                     /gene="folD"
                     /locus_tag="PLQ30_00205"
     CDS             complement(40948..41832)
                     /gene="folD"
                     /locus_tag="PLQ30_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HPW00894.1"
                     /translation="MSAVLIDGKKIAEEIRNELVQKVNFLKGKGVVPGLAVILVGDNP
                     ASISYVTAKEKACAEIGMKSFETRMAENSSEQALLDRIMECNRDPNIDGILVQLPLPR
                     HIDERRVIEAIDPNKDVDGFTPVNLGRMLLEQPSFIPCTPFGIIELLKRSGVPTNGAR
                     AVVVGRSNIVGKPLANLLLRKSVNATVTICHTGTKNLGSHVREADIVVACAGAPGLVS
                     VDMIKPGACIIDVGVNRVPDSTKKKGYRLCGDVDFEGASRIAGWITPVPGGVGPMTIT
                     MLLSNTIDAAARISNIAI"
     gene            complement(41874..43082)
                     /locus_tag="PLQ30_00210"
     CDS             complement(41874..43082)
                     /locus_tag="PLQ30_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPW00895.1"
                     /translation="MSYIFSRRSNAGEIEPNELSRVRARRHGFGLPIKDLSNTNPTKV
                     GLDWKDHDLISLAIKDNVTYDPEPRGLYRAREAISDHFALYNRNMHPDNYFLCASTSE
                     AYSWLFKLLCDPGDAVLVPKPGYPLFQHLTELDSVQVLAYRLEYAHPSGWYVNLDELS
                     SVLAADREKRIKAIVIINPNNPTGSYIRKTERTTIIGLCEKYGLALIADEVFFDFPID
                     DNIERKTFVGEDRVLTFVLDGLSKRMGLPQLKLAWITLSGPNQEVSRAKKRLDLIADT
                     FLSVSTPIMNALPFLFEHETAFLCKSLSRIRENYASYREILTGGTSPHRVLCCQGGWT
                     ALIESPSFFEEDRIAQRLLEEKGIFAQPGFFFDIDNGVHFAFSLIIPSISAFRWCTEY
                     RHFFEALENS"
     gene            complement(43093..44748)
                     /gene="ftcD"
                     /locus_tag="PLQ30_00215"
     CDS             complement(43093..44748)
                     /gene="ftcD"
                     /locus_tag="PLQ30_00215"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPW00896.1"
                     /translation="MEKPLVECVPNFSEGRDRAKIEAIADAIRSVQGVFLLDVDPGVD
                     TNRTVYTFIGPPEAVKEAALEAARVAFKLIDMRTHRGAHPRIGALDVCPFVPVSGVSM
                     EECIEISRDFGKKLSEELNVPVYLYEKSATRPERRSLADIRAGEYEGLENKLADPAWQ
                     PDFGPTHFTPRWGATVTGAREFLIAYNINLNTKDKRVAHDIALSIRESGRTIRDSNGN
                     TIKVPGLLQAVRAIGWYIESYQCAQVSINILDFRTTPLYVVFDAVKKEAEARGIYVIG
                     SELIGLIPLEAICVCGRYYRQKAGKSPGLSDEELVELAVQTLGLRSVQPFEPEKKIIE
                     WAIRKEGALVSKKVSSFVDTVSSDSPAPGGGSVAALAGALGSALAAMDANLTVGKKGY
                     EGVSETLSTLGIKAQRLKNDLLEIVDEDTAAFNAVMDAMRLPRSTDAERIARDLAIEK
                     ANKLAAGVPLKTARFCLEAMRLCLEAVETGNLNSATDAATGCLMAWAGLSGAVLNVRI
                     NIKTLSDEQFACTASKECDSLEIEATQLRQDLEKSIPSILGLR"
     gene            complement(44750..45442)
                     /locus_tag="PLQ30_00220"
     CDS             complement(44750..45442)
                     /locus_tag="PLQ30_00220"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HPW00897.1"
                     /translation="MADTKAEQEVLGLIAEQRLPFSAVILNGGHARRFGGIDKQEVEI
                     EGVPIGFLLAYRLGKIAQELLIVGKPHKLYEDLGASQFEDDIKEGGPVCGLFSALVNA
                     SLEWVCLCAADMPFVSLALIRYLYVQSMSKEVDIVLCKTDDTLQPFFAFYKRNLVSEL
                     YSYIQSSSDLSFRHFIKMEKHLIIQENDIASICDPGIVFQNINDRKSLERARKVANRQ
                     VLGLDEFHIMEE"
     gene            45454..46350
                     /locus_tag="PLQ30_00225"
     CDS             45454..46350
                     /locus_tag="PLQ30_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HPW00898.1"
                     /translation="MPCFTKFMQILKWADWSAPPEQMNCSLSFQCIIQTIKELIPAIE
                     SAQLSDEKLDSYRIQELLDKAIRLYLLTPALVNVLLNYKICVEHDLPLHPTVYYELKE
                     ARKYRIRHSLAEIQQANEQYWQSIEVARLCYQCAPQAISAIDELCFGIPSGIASFLYT
                     AVQDHYTWLGSQPSLLLELAEKISREFRPSLIVAAAHGSIMPALILSELLEIPVYFIR
                     FSMFKRHDEEPIISLSDQAWLFDYRNKNVLLYDEDVARGNTLDLFSRRLSPLFGEVRT
                     ACSIRHAGSCVHADFSGRVWWD"
     gene            46670..47314
                     /locus_tag="PLQ30_00230"
     CDS             46670..47314
                     /locus_tag="PLQ30_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPW00899.1"
                     /translation="MSQKTKLNYVPSIRRFPSYLHIIRAFQRTGEPYISGTLIANELN
                     LEPIQVRKDLSITGIVGKPKKGYPVDQLIAAIEHYLAWDVEQKAMIVGAGNLGSALTG
                     YQEFRNHGLHIVAAFDSDPLKVGKNIHQIHVYQMNQLREIADEMKITLAILTVPSPYA
                     QSSCDAIVDAGIKAIWNFTNVKLRVPDDVIVQREDLSSGYAILSVMMRTKARLS"
     gene            47338..47919
                     /locus_tag="PLQ30_00235"
     CDS             47338..47919
                     /locus_tag="PLQ30_00235"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HPW00900.1"
                     /translation="MRIFLISGSRCSGKTSFCNSLIKSAEDKGIVCRGCIEISQRSEN
                     GIPHSIDLYNVSNGDTFLAASRPIDRGDVPFEYSPDAFAHIRKSYIQTVESDIISQHP
                     QNIPDKKREICLIDEIGPLELEHRGGHLSLLEFVLGTKSPDALVLTVRTGLKYSLMSF
                     LINHGISADHIYDLELEKLTISSACIYVLSFLE"
     gene            complement(48032..49480)
                     /gene="pyrF"
                     /locus_tag="PLQ30_00240"
     CDS             complement(48032..49480)
                     /gene="pyrF"
                     /locus_tag="PLQ30_00240"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPW00901.1"
                     /translation="MGFFEYIEERADKMDTFLCIGLDPAFSNSEIEKRGSLECAACAL
                     EQNLRIIEATASYALAFKPNIAFYEAMGNAGMSALYKTLAQIPEEIPIIIDAKRGDIA
                     NTAEAYARAIFGELKADAVTLNPYLGLDTLEPFLRWSDRGIFVLCRTSNPGAGFLQDI
                     TVNKSPLYSMVAQKCSDLSVDVGLVVAGNDLEALQKVRNVAPSAWFLSPGIGAQGGRA
                     DLAFAAGARSDGKGILVAAARSISEAQRPDDAALELRDVLRRARDAFLFKAAVKTAKP
                     SKKAEDEVASLKREFVDVLLSTECFRLGEFVLKSGKKSPFYIDLRNLISDPEAMRIAG
                     RAYAHLASSCDFDIIGGIPSAGLPLATVASLELGKPMIWPRMPAKDHGTGKRIEGRYA
                     KGDRVLLLDDLITTGASKIEAKDILKSEGLEVRDLVVLIERGSEGRVDMEREGVRLHA
                     FMHVNELFEALKENGRISEAQYSDLVDFGKTI"
     gene            49576..50937
                     /locus_tag="PLQ30_00245"
     CDS             49576..50937
                     /locus_tag="PLQ30_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW00902.1"
                     /translation="MKKATLLFALVLFSFPIFAQQGMNPSFGIIANLGTDLLPDPGDP
                     SKFESWSKIGMQPEFSIGKFGIGLDLLFRFKLGTGSNTSLEIYEPDWIPQQGQNIFDV
                     YLPKILYIRYGQQWEDPFYVKMGSISDFSLGNGLIVDNYSNMRFLPQRRVFGMQLGID
                     GSLFGFPYVGLEVLTGNVSKFDVIGGRIYVRPLAFMEESLFGRLQLAATGVFDRDPLL
                     YTGTPYLAQQTRLIYVVGADITLPLIQSPAFSLTPFGEGAYEMNNAIGAIAGIKGRAF
                     DILSYLAQFRYLQEGFIPSYFDANYDIYRADRFVYIETNPAKNNFIPGWLASLGLDLF
                     KSALSFGVILDAPFKELPSPATDNPADYAHIKGSLNLARGLIPNVNIAARYEKFFLGR
                     KSNDVLKDLIDFEDSSIGMTISYKAGTAIVSMDYAYIWNPTKNSGAGAFDVVSSMSLG
                     FEL"
     gene            51029..52795
                     /gene="mutL"
                     /locus_tag="PLQ30_00250"
     CDS             51029..52795
                     /gene="mutL"
                     /locus_tag="PLQ30_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPW00903.1"
                     /translation="MGNIRILPPETSRRIAAGEVIDRPSSALREIIDNSIDADAHNIS
                     VSIAQGGIEEITVADDGWGMSKEDLLLSISEHATSKIYGPDDILKAKTLGFRGEALAS
                     IAAVARLEIVSKLRGSNEGWRLSSSPLKEPVIEPFACKEGTRLTMRGLFESYPARRQF
                     LKRPQSEAFLCRSTFIERAVSHPDICFRWAVSSDMDVMIASSLKDRILRCYTELSRFT
                     LFEAMAETSELQMHCIYADVANYRRDRKFLQVFVNKRRVPEWNLLSLMEYEFGKYLPG
                     GAHAIAFLFIEIDPSLADFNIHPAKKEVRLKKLAELRTVLHELLSHELVHRYGGMTQA
                     FETAIYPEFKEFVFSTKEKQIAPGLADQPQVLHSTKYDREMGREFTNCSSNSYFVRRD
                     STQLPDDFWHRIKDDNLSTLRYIGKGPGPFLIFELSDALFILDQHAAHERILYNKIKS
                     TRGESQLLLVPYLLEPYENEHYLEESKDNLEFVGYGITRDKNTWIVEAVPAIAASEAL
                     EALVEWLSQPCFETSPIDALIASLACKAAIKDGDRMDEISALKLIQESLELPEPRCPH
                     GRPIYLAFPRQQLYSMFGRTIG"
     gene            52792..53460
                     /locus_tag="PLQ30_00255"
     CDS             52792..53460
                     /locus_tag="PLQ30_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPW00904.1"
                     /translation="MIRPVRILCVADEVDLLVYSVQICERFSDVDLILSAGDLPDGYL
                     EYIASMLNKPLISVAGNHDKSKQDIENAGIHYPRNQTANFGQISFRIEKESGISILGL
                     PGSIRYNKGQNQYTDAWMTFRIILMLPRLLLRRLLWGRSVDIILAHSPPRGIHDGGDP
                     AHKGFSAYRWLIRLAKPYYFVHGHVHVYDMQALREIDYYDTSVVNVYGHRVITLPRED
                     KNGR"
     gene            53450..54388
                     /locus_tag="PLQ30_00260"
     CDS             53450..54388
                     /locus_tag="PLQ30_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPW00905.1"
                     /translation="MADDLHALAESDFYKARSKAISASIFGMLRPSMSDLMPFDQAKE
                     LLKPRAETYRGVTAVPIDRIVGSEGRYRDFNRFFFPKKEHLKARWTTIDELQYKDVSL
                     PPVVLYEMGGVFFVRDGNHRVSVARAKKQEFIDAEVISLHSEIQLDPEMTIEDIKKAV
                     IEYEKKQFYRETNYPNIVGADDLNFSEPGRFDSIKEHVLVHKYFLNQHLPEEIPFHIA
                     LYSWHDNVYQPISQSIEAENILSLFPGRTVSDLYLFLVAHWDELKRKSGGYVAIDEAA
                     ESFKNEIKRNRNSSFEHACRIFGSISKKVENFIAKL"
     gene            complement(54836..55597)
                     /locus_tag="PLQ30_00265"
     CDS             complement(54836..55597)
                     /locus_tag="PLQ30_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPW00906.1"
                     /translation="MLINGIVVLIIVSMLLLLEYILALRCLFADRYERFHLCAKYEQI
                     GAKIILGLFRVFRGFRIRVHNPERLEIPLRCLVISNHQSLLDILVLIAFLGIERKPRF
                     VAKKELQYGIPFVSFTLRKGGHCLIKRKGAPLDAMREVSKMAKICAKEGACPVIFPEG
                     TRSRNGHVGEFHTAGVRKLLESGGRLSVAAIALEGGWRVANAMDFLKLFGKTPYIVEI
                     VKIYEAPRNKYDIENMLIDARRLIEERILELRRLQ"
     gene            55743..56012
                     /gene="rpsT"
                     /locus_tag="PLQ30_00270"
     CDS             55743..56012
                     /gene="rpsT"
                     /locus_tag="PLQ30_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPW00907.1"
                     /translation="MSTTNLSAEKRQRQNEKRRLANKSTKTSIKSAAKKVVMASEKKD
                     TEAAKQALSEMIKRIDSAARKGIIKKNTAARKKSRMQRLVNKIDQ"
     gene            56054..56398
                     /locus_tag="PLQ30_00275"
     CDS             56054..56398
                     /locus_tag="PLQ30_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPW00908.1"
                     /translation="MAEKLTKAELIDALYDSLSSTSRTTRKEIHELIDGLFAEIKGAI
                     LDGKIVELRGFGTFEVKLRKGRSKARNPKTGEIVSVTDHGVATFRPGRELKRAAWEMD
                     IERIPKSFRKRV"
     gene            56459..56845
                     /locus_tag="PLQ30_00280"
     CDS             56459..56845
                     /locus_tag="PLQ30_00280"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPW00909.1"
                     /translation="MKKSNLEPSEPYATHIGPALKLNGILRSNQPLDLHCTFHGSIEG
                     TFIKISKNAIIEADLLKAHQVVIAGQFKGSMSVSEEIIILPNANVSSDIKVACLHIIE
                     GSQFEGKIEMAGFYPDHNSPIVDRGH"
     gene            57035..58783
                     /gene="rho"
                     /locus_tag="PLQ30_00285"
     CDS             57035..58783
                     /gene="rho"
                     /locus_tag="PLQ30_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPW00910.1"
                     /translation="MAIHNRKKIDSSQTTSSNNDENNSAEPREEIAMNQSQPEEQPVL
                     KNESLFEEQTEDLVQDIQRSLNNVHVAAPVKNQQQKTRKPVAKIRLKKKPTQKTGLLE
                     ETQVPESTTTSEVIEEQPNGFVVAAMPLDEHQNQRLSELPKPEPENRTEQRVESRARL
                     LINDLSKMGIIELRELGAKYGINHDLLITLKKQELIFNILKGHTEHGGIIYAYGSLEI
                     LPDGYGFLRSPQNSYLPGTDDIYISPSQIRLFNLKTGDTVYGQIRSPKEGERFFAMLR
                     IEQINFDDPSVAQNRIPFENLTPLYPTEKLDLETSTEEISTRIINLFCPIGKGQRSLI
                     VSPPRTGKTILLQKIANAITANHPEVYLIVLLIDERPEEVTDMERTVKAEVISSTFDE
                     QATRHVQVAEMVLEKAKRLVEHKRDVVILLDSITRLARAYNQTVPTSGKILSGGVDSN
                     ALHKPKRFFGAARNIEQGGSLTIIATALIDTGSRMDEVIFEEFKGTGNMEINLDRRLS
                     DRRLYPAINIKKSGTRKEELLISEEELQKIWVLRKVINPMDDAEIIELLIDRMTKTKN
                     NDAFLKSMNTPYSSME"
     gene            58886..59101
                     /gene="rpmE"
                     /locus_tag="PLQ30_00290"
     CDS             58886..59101
                     /gene="rpmE"
                     /locus_tag="PLQ30_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPW00911.1"
                     /translation="MKKGIHPKYDITTITCACGNVIETRSTAKNIQVEICSACHPFFT
                     GKQKLVDTAGRIERFNKKYGIKTNENK"
     gene            complement(59182..59787)
                     /gene="rdgB"
                     /locus_tag="PLQ30_00295"
     CDS             complement(59182..59787)
                     /gene="rdgB"
                     /locus_tag="PLQ30_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPW00912.1"
                     /translation="MRFVVATNNLHKIVELKPLFPCHELLMPSDLGINEFCPEENGKT
                     FFENAYIKARSLYELLGIPSMADDSGLCVEALGGKPGIGSARYGSNGKELLAASEKNS
                     LLLSELAGVSNRNCAFVCCLVFYYGKQRFMCVQETLEGEIAGSPSGKGGFGYDPIVYL
                     PQYGKTVAELSADEKNRISHRGKAAQRMATMLATLSLGCRD"
     gene            complement(59797..60639)
                     /locus_tag="PLQ30_00300"
     CDS             complement(59797..60639)
                     /locus_tag="PLQ30_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW00913.1"
                     /translation="MDSQTYLDLVRKMYEHPKRYGFFTKDEISDALNNYRNRIELILE
                     RAENESRTKDAYLLSSMRYVAKSVHRQNYNLSLCETAYIYSNFTEELAIESPVEPWTY
                     YETINDATTEKGILNLPPKVILDKLSPAHKRLLFLTMKCAWEIDEELLSRCSLSLGIP
                     REYLFNLIELTKRRTERGWNKAEQMNIKLHALWIRLRVLEFRLETCLLNEEKEYILCS
                     LKRCRERYARLLEKRCHRRSAVSNESISQILGVPKGSIDSGLFYLRKHVDSGRSLEDY
                     RKLG"
     gene            complement(61073..62059)
                     /locus_tag="PLQ30_00305"
     CDS             complement(61073..62059)
                     /locus_tag="PLQ30_00305"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPW00914.1"
                     /translation="MRQVPKILIDFINHHDCFYILGHREPDGDCIGSQLALASMLQSM
                     GKRVHVLSSGPFNRIEILRFEARFKSEVPSERDFERTAAIVLDCSSMERIGSVAERMP
                     NIPASFIDHHSTSSAPGPYDWLDESASAVSAMILLLMEAMGHSPTREEAELLFFGLCT
                     DTGFFRHLDEHGSESLKIAARLVEAGVSPKRVYMAINGGRTLASRRMLGELLLRIQPY
                     YEGRLLVSWVTIEDQQRYGMSSRDSDLLYQLMMGIVDCEVCFVVKQETEEMCTVGLRS
                     KNSINVARIAEQYGGGGHRLAAGLSINGKVDEVVHTLVRVFGDVFRDEEIEK"
     gene            complement(62090..63031)
                     /gene="argF"
                     /locus_tag="PLQ30_00310"
     CDS             complement(62090..63031)
                     /gene="argF"
                     /locus_tag="PLQ30_00310"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPW00915.1"
                     /translation="MNPSILKGRSLLTWIDYTPEEIRYFLSLSKKVKEEAKKGMHKQR
                     FKGKTMALLFEKRSTRTRCAFETAFGEEGGHPVFLSNQDIQLGAKESIEDTARVLGRM
                     FDAIEFRGYKQEYVEILAKYSGVPVYNGLTDLFHPTQALADVLTIEENLGNCAGKRLC
                     FIGDGRNNVARSLMVISAKLGMHFTIVSPRELWPDNELKNLCEGFAKTSGSTIIITDE
                     IESGVNGADAIYTDVWVSMGEEALKAERIRMLSPYQVNAELMAKTGNKACIFLHCLPA
                     VKGEEVTFDVIEGPQSKVFEEAENRKHTIKAIILATL"
     gene            63119..63829
                     /gene="nth"
                     /locus_tag="PLQ30_00315"
     CDS             63119..63829
                     /gene="nth"
                     /locus_tag="PLQ30_00315"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPW00916.1"
                     /translation="MSNELEISLKEINESIPLPQLLEELYIRLVPIWPDTRPLLHYNN
                     CFELLISVILSAQTTDDQVNIVTKELFHRFPNSESLAAADIHEVENIIYPLGFYHVKA
                     KHIIETAKVLQGRFNGQIPSNFEEMIELPGVGRKSANLLIAACYEKPGIIVDTHVLRV
                     LFRLGVSAKKEATLIESIIRANLPPEKHTEFSYAVNRHGKFTCTARKPACTEQKKHCP
                     IDDICPKIGLSALLKQRR"
     gene            63859..65529
                     /locus_tag="PLQ30_00320"
     CDS             63859..65529
                     /locus_tag="PLQ30_00320"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HPW00917.1"
                     /translation="MFKPDLVSESEDPIGILGRDRFHIPHLTPLQRFVIANILDETKG
                     STVLTRNLSSDPIDDDAVSNLNKENEFQERQETYRQLILFPTGFGKSVCFQLPSLLLD
                     GLTIVVYPLLALMNDQKRRLDEANISCALFRGGLSQEEWNEEEKLVSSGKANMVVANP
                     EILSAPKLRRFLNRFKIAHFVVDEAHCISEWGESFRPSYLALGETVEAISPGILSAFT
                     ATAGPHIVHSIEKRLFMDKDYRLVDSEADRSNILYAVVHTLSVTRTLRQTLLCCKKPA
                     IIFDRSRPGTRLKAELLKASGFSQARFYHAGLDRAERCNIEKWFQDSDDAILVSTNAY
                     GMGMDKKNIRTVVHSSLPESAEAYIQEAGRGGRDGKNALAVLIDDLAVRYYRENLKDK
                     NIQDIQSIRKACFLPYPTLSSCRREFLLHLLGEEKTPECGRCDNCIEGSHTQRTQFNS
                     DASDILPEWKFLSRAEGFIEAISFCDSHHGVFTSQELVDALGPHGKGKGEYAGCLYGW
                     LKIEREELINALFLLDILYAPKKGTRKNRILLSQKGKALSRLARNSLV"
     gene            65612..67219
                     /locus_tag="PLQ30_00325"
     CDS             65612..67219
                     /locus_tag="PLQ30_00325"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPW00918.1"
                     /translation="MVVFKNLDTCQAFEALKKIPAVVLHKSLDQKRISDWWIPHAAGF
                     CYCYAFAPVDDSVIDWLEALTREQQLIEKYRILTEGAIMNTGEKRMVLHHLVRGRLGK
                     PVIWNGQDMEVFYREEKHRFYDFAHKIREGEILGTTGKAFTQIVQIGIGGSDLGPRAA
                     SLALSRWAVAAGRARLRPWFISNVDPDDADYVLANIDLSESLFILVSKSGTTQETLAN
                     ETIVRDRLQKAGLNPDKHMVAVTSTSSPLAHNPHYLDSFYIDDFIGGRYSTSSAVGGV
                     IISIAYGPETFEEFLEGANEADRLAFEPSLRKNPVLMDALIGIYERNILGWPATAVLP
                     YSESLSRFPAHLQQLDMESNGKSVNREGHPISYSTGPLVFGEPGTNGQHSFYQLLHQG
                     SDIIPLQFIGFLRNQLSPDMISEGSSSRQKLLANLIAQILAFAIGKKDENPSKTFSGN
                     RPSTLLLARELGPRQLGALYSHFENKVMFQGFVWNLNSFDQEGVQLGKILAKKALSAI
                     TKGESTDDELLDAFIASALRAVENNGD"
     gene            67264..68493
                     /gene="murG"
                     /locus_tag="PLQ30_00330"
     CDS             67264..68493
                     /gene="murG"
                     /locus_tag="PLQ30_00330"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPW00919.1"
                     /translation="MQSIYETELQEKDELENDCIAFTGGGTGGHIYPGLAVIEELRRL
                     GFKGRIAWIGSEKELDRSIVTGQGIEYHAIPSGKFRRELSLRNISDLGRVASAYFRAR
                     ALLKDIGPSILFSKGGYVSVPPCWAASSLGIPVITHESDASPGLATRLNSKKASLILT
                     SWEVTAQYFPESLRTKIIRAGNPVRPDILNGDRQRGLSMLEFVAEKPVLFVLGGSQGA
                     QEVNDIVLSSLNLLVPLVQIVHQTGRANFDEVKARVPDDPAVRRAYRPFPYIGSEIAD
                     IYAASSMIAGRAGAGTIWEAASLGKPMILIPLSGSGTRGDQVENAALAEHSGIATVLL
                     KPNISSDLFVDAVKKYLNIKNYAMAEEACRNLVHIVKTPHIPEITDGPSQNPILSAEY
                     IAQLLLTKMAEWAKESA"
     gene            68490..69023
                     /locus_tag="PLQ30_00335"
     CDS             68490..69023
                     /locus_tag="PLQ30_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPW00920.1"
                     /translation="MSTIDWVFSGIVIILAARCFVRGFIHEILSVASIALGILAGLLL
                     SNTVILQILPKINADKLPYQAQYIIAFILCFIAGFLLMKIIERMLRQGLEASNLDILD
                     RILGLILGVAEGFIVVGLFIVILEIQPIVDAKAILSNSLYVKLLGPIVEPAIGGTLSP
                     MLKDMDMPILKQKPKGK"
     gene            69026..70324
                     /locus_tag="PLQ30_00340"
     CDS             69026..70324
                     /locus_tag="PLQ30_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW00921.1"
                     /translation="MFENLLFQEHALEQLVSLAKEKKVPPALLFVGPAGSGKLTAALE
                     CARVLSCEKEAEWNCECTQCRCHRNLMHPDLLLFGPREFAQETIVTGEYLIGKPGLTS
                     YYAFVRSIRKLLKRFDSVLWQGEEARLSKAIPSIETCEELIQEISELVLKKKNHALTV
                     AVENAINAAAGLESLVPDGIPVFMIRNMISWAVLAPSAKTKMIIIQNADAANESARNA
                     MLKILEEPPETVRFILTSSRRAAVIATILSRSRLISFDSRTGDQAREIISRLFHVDED
                     LQNINSISEFFQNKSPYPPKVATRDAALFIGALLGKTMKENPGLSGKYAIELASMAQC
                     ENVSALSILRQVSDKTNAFGAKNSRFANSFLVFMRAVSQHLAHIAADPSSSPSLIMYV
                     DRLFSKLREMNMQYRTLNRSPELMLEAFADILGEQYESRI"
     gene            70308..71501
                     /locus_tag="PLQ30_00345"
     CDS             70308..71501
                     /locus_tag="PLQ30_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW00922.1"
                     /translation="MKAAYENTLKRIDRIPPEGLKQIVRNLLAENSIYESALNSTLDG
                     IIVCDLAHKPLFINRSAEKITRIVSWAQEVVLWEQLDDDNLADFIQEALSSEKNILNH
                     ELSISRPGGTRLLSISISALVSEGKIAGNLIHIEDITEKRRREAQLRRAESLAALTTL
                     AAGVAHEIKNPLASIRIQLGIIRRILEKNCAKKTEPIFHNISLVEQEIDRLNSIVVDF
                     LFAVRPMDITLIRNRVEDVIKEVVEIMGNEASVHHINIVCEFEDNLPEVMIDPKHLKQ
                     ALLNIIKNAIAAMPNGGTLTISAVTRHDELQISISDTGIGIPEELLAKIFEPYFTTKE
                     SGTGLGLTITFKIIKEHNGEITVESKPSKGSTFTIHLPIPQPEQKSLPPWEDGLQIEG
                     SEREE"
     gene            71502..72902
                     /locus_tag="PLQ30_00350"
     CDS             71502..72902
                     /locus_tag="PLQ30_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPW00923.1"
                     /translation="MRATLLVVDDEINIREGLAQLFSLEGYDVKTAKDGLEGKMIVDK
                     EDIDVVITDYKMGRMSGLDLLQYIVSTHPGIPVIILTAHGSVDKAVEAMREGAFDFIA
                     KPPNTDHLINLVRRALETRELYRTNIELKAEVESQRARSYIIGKAPSIKRIFDLIRKV
                     APTKASVLITGESGVGKELVADAIHNLSPRRDKPFIKVHCAALAESLLESELFGHEKG
                     AFTGATGRKRGRFEMADGGTLFLDEIGEINQNVQIKILRVLQERKFERVGGEETIEVD
                     VRLIAATNRDLKREITEGRFREDLYYRLNVVNIHVPPLRERREDIPLLAMAFLREFAD
                     ENGKQIEGFDPRVRQALYAYDWPGNVRELRNCIESAVVMASGKLITLDDLPPGPRSSG
                     EARTISIPAFSSLEEAERIIISETLALVGGNKSKAAEILKIGRKTLYQKIAQYGIGKQ
                     EANSSVSSNPREETEN"
     gene            complement(72934..73713)
                     /gene="whiG"
                     /locus_tag="PLQ30_00355"
     CDS             complement(72934..73713)
                     /gene="whiG"
                     /locus_tag="PLQ30_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HPW00924.1"
                     /translation="MDYATLEEDRLWELYRRTEDIHIRNEIIMRYAPLVKYVAGKVSV
                     TLPSMVEYRDLVGYGTFGLFDAIEKFDPNKNVKFKTYAVTRIRGAIYDHLRELDWVPR
                     SIRRKTKQIERAIAELESQLGRPATDEEIAKEMGIDCKELLKIMSKVASSSILSLHEI
                     WPINSEAEGYTIEDSLESTRLENPDLVLEREEIRRVIFEALQELPEKEKKVMVLYYYE
                     NLTLREIGKILEVTESRVSQLHTKAILRLRAKLSNKKRGII"
     gene            complement(73737..75104)
                     /gene="hslU"
                     /locus_tag="PLQ30_00360"
     CDS             complement(73737..75104)
                     /gene="hslU"
                     /locus_tag="PLQ30_00360"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HPW00925.1"
                     /translation="MIIEKDALTPHKIVEELDRYIVGQEKAKKAVAIALRNRIRRQCL
                     PPEIREEIAPKNILMIGPTGVGKTEIARRLAKLCNAPFLKIEATKYTEVGYVGRDVES
                     MVRDLMAAAVSMVKEEMDSQVATEAKRRAEERLLDILLPGLAKSDEPIGQSQATLIIP
                     NSGMETKEKFRTLLKEGKLNSRQIEITVPAQGPQIELFAGTQFEEMNIALGGLQGLFG
                     GSKKKKTVSVAEALPILEAEEKEHLVDQEKVVQEARRRTQELGIIFIDEMDKIANRDA
                     RGSSGIDVSREGVQRDLLPIVEGTTVNTKWGPIDTTHILFIGAGAFTVAKPQDLIPEL
                     QGRFPIRVELDALTGDDFVRILTEPKNALVTQYIQLLSTEDVQLEFNGDAIARIAEIA
                     AAANRQSENIGARRLQTVMERLLEDISFDADKHTSTKVVIDRAYVDERFKDYVQQQDL
                     SKYIL"
     gene            complement(75101..75691)
                     /gene="hslV"
                     /locus_tag="PLQ30_00365"
     CDS             complement(75101..75691)
                     /gene="hslV"
                     /locus_tag="PLQ30_00365"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HPW00926.1"
                     /translation="MELHATTILAVRKDGHVAMAGDGQVTMNNTVLKSNARKIRTIYN
                     GKVLCGFAGATADAFTLFEHFEAKVQEYGGDLTRASVELAKDWRTDRVLRRLEAMLLV
                     ADQSKMFLLSGTGDVVEPSEDAIAIGSGGSYAYAAAIAYLDASRRMKEAARSTPGEDA
                     GMLPDSFSAREIAERSLKIASSICIYTNDQIIVEEI"
     gene            complement(75709..76605)
                     /locus_tag="PLQ30_00370"
     CDS             complement(75709..76605)
                     /locus_tag="PLQ30_00370"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HPW00927.1"
                     /translation="MDARLLDYLDYLASVRGLSIRTVEAYRRDLAKYEIFLEGKDLDE
                     ADASDIRTFAGTLVMEKRAASSINRALSAVRGFYRYWMKFHKKGKDPAREVENVTVGR
                     TLPSFLFEEEINSLLDGLGGDTFLDVRDRALLEVMYSTGSRVSELCGMRLSRLNTNNC
                     TIRVKGKGSKERLVFLCDAANTALGQYLPYRAAILRKYGIEEHDRIFVNSRGRPLSSR
                     GVEKIVENRRLKAGIRKHLTPHTFRHSFATHLVAAGADMRVVQEMLGHSNISTTQVYA
                     HVDMERLRRVYEQAHPHGSKTK"
     gene            complement(76609..78771)
                     /gene="topA"
                     /locus_tag="PLQ30_00375"
     CDS             complement(76609..78771)
                     /gene="topA"
                     /locus_tag="PLQ30_00375"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPW00928.1"
                     /translation="MTAKKSKEKKTPEAAKAAETHKLKTLVIVESPAKARTIEKYLGS
                     RYKVLASNGQVIDLPKSRIAVDVAHNFEPEYITVRGKAEKLKELTEEAKRSSAVILAA
                     DPDREGEAIAYQIGKYLKDRAKDTPISRVTFNEITKQAVQDAMANPRDIDMNLVEAQK
                     ARRIIDRLVGYTLSPLLWKKVKSGLSAGRVQSVALKLICDREKEVESFIPEEYWTIEA
                     HLKAHQHIVRAELALLNGEKPSIKSEAEANNILALFLGKPALVSDIQSIDRTVKPKPP
                     FTTSKLQQAASNRLGFTSTKTMKIAQQLYEGVDMGHQRTGLITYMRTDSTRISEAALH
                     EIYEWLTKYFPNQTPAAPIRYSVSNAAQDAHEAIRPTRVDITPDEAGRYLKGDQLKLY
                     TLIWEQFVASQMKPAIIRTLTADILIGDGIFRTSASSFLEEGFYKVIRLAASKEERAS
                     HHLPFEKGETLQVERIESEQHFTQGPSRYTDASIVRTLEELGIGRPSTYAPTIETLIE
                     RYYVHRDKRQLVPTQLGRIIDDLLSQNFPEVINTSFTAEMETMLDKVEEQKIDWVSEL
                     KKFYFPLVDKVENALNVLEDMHGALDEKTDEKCPICGRPLVKKLGRFGYFISCSGFPE
                     CTFTKSVPLATCPKCGGDIVPRVSNKGRRKKFYGCSNYPECNFKTLYKPSNSLCPKCG
                     WFLVEKYDKKTGHRKLCINPDCDHLHSSQQSGENNGES"
     gene            complement(78835..79863)
                     /gene="dprA"
                     /locus_tag="PLQ30_00380"
     CDS             complement(78835..79863)
                     /gene="dprA"
                     /locus_tag="PLQ30_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPW00929.1"
                     /translation="MKAENEIRCAYPHGGFSQMTLGKQHKGIGKLIQLELDRISAIQP
                     SMEKTPLALRLAFQRLHFLSWQERVLLLENIHDMEDFLALGLAEIESFLYRSMHARPL
                     DMGKIWEQAERDINILDRLGARFVCVVDKDYPPLLREIHRAPFGLFVRGDTKALNQAS
                     VTMVGTRSPTSRGLYTAQLLAKETADEGIVIVSGIARGIDAASHRGAIQSGAAATIAV
                     LPCGPERVYPSSNAALAAKILDCGGCLVTEYPPGVSLDRYRFPERNRILAGFSKLTLV
                     VEAPEKSGALITAEIALSEGRDVAVAASCLGSSRNSGADALKEDGALAIKAGAELVPM
                     IRCEYNGI"
     gene            complement(79882..80778)
                     /locus_tag="PLQ30_00385"
     CDS             complement(79882..80778)
                     /locus_tag="PLQ30_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HPW00930.1"
                     /translation="MSILLRRFQAYLLATRRRSVLTADAYLREIEMLENFLFQYGKTP
                     LDAGEEDLLAYLVNRSQSGLQRTTMARIIASLHSFYRFCLNEKLRADDPSIQIRTPKQ
                     DRNLPDVLDPDSIEHVLECIDITSPNGLRDRALFELIYSCGLRISEAANLKFQQLYLE
                     ERLLRVIGKRKKERLVPFGDEAKYWLSRYLAEARPLLEKRERTDFVFLNQEGKGISRK
                     GIWKRFSKIREKSGVNAKVHTFRHSFATHLLAGGADLRTVQELLGHSDIATTQIYTHI
                     DEESLMMYHKEYFPRKSEKNKA"
     gene            complement(80775..82022)
                     /gene="ftsZ"
                     /locus_tag="PLQ30_00390"
     CDS             complement(80775..82022)
                     /gene="ftsZ"
                     /locus_tag="PLQ30_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPW00931.1"
                     /translation="MAIELVDDNGIGSPTVIKVIGTGGGGSNAVNRMIMAGLKNVQFI
                     AVNTDLQDLGRAKAEIRLGIGSKITKGLGAGGKPEIGEKAAIEDRDKIEQALRGADMV
                     FVTAGLGGGTGTGSAPIIAQTARDLGALTVAVVTKPFAFEGKIINRIAEEGFSKLKQA
                     ADTVIVIPNQNLLRVVDKKTPLKEAFRIADEVLRQGVQGISDLITLAGDINIDFADVR
                     TVMEGQGDAIMGIGVGSGDNRAQEASEKAINNPLLDDACIEGARNILVNVTCGEDFSL
                     IEYQEVIDLITSKAAEDAHIIVGVVTDSSIKDEVRVTVIATGFGALHQKLGEKTVTKN
                     TIAMPLGSIRPSEFLSPGEWPLHEEKPAQKRDQASENQDKSSFKDNFGRISFDPESDN
                     DEFDIPTALRNKRLMPEDFGGRR"
     gene            complement(82044..83255)
                     /gene="ftsA"
                     /locus_tag="PLQ30_00395"
     CDS             complement(82044..83255)
                     /gene="ftsA"
                     /locus_tag="PLQ30_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPW00932.1"
                     /translation="MDIGTTVTKAIVGGFNEMGQFEIFGIGAAPSSGLRRGVVLNIEG
                     TLNSINAAIDAAELMSGIDIRQVSLALSGANIESFNTNGQIAVTGRGREITAEDIARV
                     HEAARAVSISMDREILHVVPRSYTVDDQKGIRNPLHMTGVRLECSVHIITGSVSTVQN
                     MLKCVTRAGYKVEQSYLGILGCARTVLTEDERELGCLLLDIGGGTTDLMVFSESDPVY
                     TSAIPLGGSQITGDISIMLSIPIDSAERLKKESGAAWTEAIDPDEAVIVPGFGAREPV
                     ELERRKLVSIVQPRVEEIFEMVNERLVKNGSKELIKAGVVLTGGSALLPHIDECARSV
                     FGVPVRIGAPTALPGLDVGFRSPAFSAAVGIVLLEAERIGTPEPSTRRKARKGKEGGF
                     SPIKWIKDRFF"
     gene            complement(83272..84429)
                     /locus_tag="PLQ30_00400"
     CDS             complement(83272..84429)
                     /locus_tag="PLQ30_00400"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPW00933.1"
                     /translation="MTDVRLQEQGNETKALRKKGTHSLLSKSDNYSMDIIQATTRCET
                     ALPGSSTAKSSGTRINAGEKIQISQRINTSSEQAHRATEEKAQSLSATKISVNPFIRR
                     APQDSVALSVQAEKLSESVKPAKTSMASKRHRSLHILIAITLLCVLSAGAAISLPRLT
                     KIDAVRVSGLETISENSIIESLGQIADENILSIDIEEIASSIRKNPRIAGAKVYRVLP
                     STLAIDIRERLAVASITINTEQGSKLILVDGEGIAFASLDADDSNNPELPVVSGIRFE
                     QFSLGQQLPEAILPLFANLAVIKQDSPELLRAFSEIHVERISANNIEIVLYPMHVKTA
                     VRLPLRFSAEALCNALVVLDILHARGLSDQPSEIDFHSGTVIYQAKEGVSG"
     gene            complement(84422..85591)
                     /locus_tag="PLQ30_00405"
     CDS             complement(84422..85591)
                     /locus_tag="PLQ30_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HPW00934.1"
                     /translation="MKEESKSKNLRIDSHRIDWQMVCAIIIVSLIGIMTLWSGSIGFA
                     IRNGGPNSVIIKQAILYAASLICMAVAAVIPMKLLRAVLPVAVVLCVIGLSLPIFSPL
                     GMTINRARRWIRIGSFSLQPSEIWKPVMVLYLASFLEKRKERLVESGLVALPAIVLVI
                     ISAVLIFLQQDFSTSILVLAIAFAMLYLAGTPFLFMSNLALFLILYGALMIFSSQYRV
                     QRLVGFLMPEQHTQTVNYQMYSALRAIRSGGVWGKGVGLGTLKISSIPEVQSDFIFAA
                     FVEESGFAGVLVVMGLWSFILIRAGRILIGSDYFSRLAGFGMITLLSLQVLLNLAVVT
                     GVMPTTGLALPFFSAGGSALLMSAISCGILINLSRSGQTETGMRAALRKEGAAYD"
     gene            complement(85604..86686)
                     /gene="mraY"
                     /locus_tag="PLQ30_00410"
     CDS             complement(85604..86686)
                     /gene="mraY"
                     /locus_tag="PLQ30_00410"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPW00935.1"
                     /translation="MLREFLLPLVKYFTPFNIFRYLTFRSAYAAVTALLISYVLGPWL
                     IDRLRVLKFGQSVRLDGPQSHLAKTGTPTMGGILIILSVLISVVLWIDISSVYCWIAL
                     SALIGFGLIGAADDILKIKKHSSDGLSVVQKLILQFIVSTIIVYSIYRTTGPSITKLY
                     VPFFKVHVVDLGVYWLPIAIIYVATWSNAVNITDGLDGLAVGLVIFAVLAFSVLTYVT
                     SRADWSQYLGLPYIKQASELTVFNFALLGACVGFLWFNAYPAEIFMGDVGSLSLGGVL
                     AVLSLMVKKEILLLVIGGVFVVEIGSVMLQVVYFKLTKGKRLFLMAPLHHHFELSGLK
                     ETKVVVRFWIMGGIFALIALSTLKIQ"
     gene            complement(86676..88145)
                     /gene="murF"
                     /locus_tag="PLQ30_00415"
     CDS             complement(86676..88145)
                     /gene="murF"
                     /locus_tag="PLQ30_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPW00936.1"
                     /translation="MEDNSIASDQQVLFYAGELAGIIGATCKGELNTPICSVVTDSRL
                     VEPNSMFVALKGERTDGHHHILEAIKNGARTILAEIDEKDAAIASIRDSGLVAETCLL
                     LIDSPLSGLQNAAREYRRRQNLLRIGVTGSSGKTTTKECIASMLSACFPEESIAMSPG
                     NLNSDIGLALAIFGIEPRHKIGIFEMGINRKGEMDELAKMYEPEIALITNIGSAHVGM
                     FGSRDEIAREKSRIFSYFDGQQKALLWEDDDYRTYLSSKVKGSVYYFGLRSTEGLGRI
                     ENKGLDGWKIEWKGQTLDFRLPGKHNLLNACAALSIAILMELDPILAVQGLSKVQPLF
                     GRSEICKGKITVFQDYYNANPNSMAAAIDFLSGISSTNRKVLILGSMLELGKESDNAH
                     RDVGFMVAEMKPEAVFLFGEEMKAAEAALKEAAFRGVLMHTTDMGNLIDTVSSYVREG
                     DLVLIKGSRSMALERISHAFQEKGLIDLSESSKGGSHAS"
     gene            complement(88132..88437)
                     /locus_tag="PLQ30_00420"
     CDS             complement(88132..88437)
                     /locus_tag="PLQ30_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW00937.1"
                     /translation="MNARRFWFSGIIICLLFIALVYQTTRYAALRAEVRELERAELDL
                     LKVGRELDADITKLANLERIERAAIRKGMIVATPEQRIIVTERQEQKASEVSNNGRQ"
     gene            complement(88434..89399)
                     /gene="rsmH"
                     /locus_tag="PLQ30_00425"
     CDS             complement(88434..89399)
                     /gene="rsmH"
                     /locus_tag="PLQ30_00425"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPW00938.1"
                     /translation="MDFFLHTPVMLEQLCNLASTIKPMTLYVDCTIGAGGHAEALLKK
                     YEGLRCIGIDADPDACNRAAERLKPFADRLLIINGYYDEVLSDIRNKRAGYCIPGDFS
                     SCLCNENGESRFSLVLFDLGISMYQLRSSGRGFSFYADEPLDMRLYPYSQCTAGELVN
                     RLSEMELADAIFKFGEERFSRQIAHAIVQKRKKERIRTSSELASIISGAVPVKYRNGR
                     IHPATRTFQALRILVNDELGRLERACIDALNLLSPGAVCAVITFHSLEDRIVKNIFAE
                     EAKNSHFSLEWKKPQSPTLEELRNNPSSRSAKFRAIRANAREEQK"
     gene            complement(89467..89910)
                     /gene="mraZ"
                     /locus_tag="PLQ30_00430"
     CDS             complement(89467..89910)
                     /gene="mraZ"
                     /locus_tag="PLQ30_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPW00939.1"
                     /translation="MKSLTGEFRNTLDEKGRLSLPARLRANLSSTLVLTQGVDPCLWL
                     FVPEEWEVLSQRILATTSLFQARSRLIQRRIIAPAQEVEVDKLGRIFIPQSLREWAGL
                     SKECVILAISRYIEIWDCQRYQAYMAEKQSEFLMAAEELGGLTLS"
     gene            complement(90080..91333)
                     /locus_tag="PLQ30_00435"
     CDS             complement(90080..91333)
                     /locus_tag="PLQ30_00435"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPW00940.1"
                     /translation="MSEISLLNALLHGFDVVVRDPVWGDVHMDSSLHALFQSRAFMVL
                     DRIRQLGPVALVYPGATHTRKAHSLGVYHIARRMALALVEKGEIDFVSKEGLRSFLVA
                     SLCHDIGHYPYAHSLKELPLERHEALAGEMIISSPLREHILECGADPEQTASIIDPDR
                     TENKSRETLLFCSFLSGVLDPDKIDYLTRDAFYCGVPYGIQDADYIMRRLIVHDDRLA
                     IDLKGEMSIEALIFAKYQMYRAVYWHSVVRAATAMVRKAVYLGLASNVLIPSQLYGID
                     DTNFNRLMSSPRFPPFRLAREAEEKRVYKLAAEHVFDESNPLHRDLLDIVKRTEAESA
                     LAQAADIAENDVVIDIPERIKLETDLMVQVDNSFEPFDQRSTLFCPNIVEKVAEALRT
                     IRIYIRPIDGIDSSKIASLVDTLLD"
     gene            complement(91430..91912)
                     /locus_tag="PLQ30_00440"
     CDS             complement(91430..91912)
                     /locus_tag="PLQ30_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HPW00941.1"
                     /translation="MNADLIRTIVLSILPVSELRGAIPYAVYKGFSIPIAAIISIAAN
                     ICIPIIAFLFLESLHRLLYKIPQYKRFFDRVVNRARAKVHTKVEKYGYWGLLLFVAIP
                     LPATGAWTGTLGAWILGLSHKKAFFAIAGGVILAGIIVSILVALWGVGAQTIFLRPVN
                     "
     gene            complement(92038..>92964)
                     /gene="miaA"
                     /locus_tag="PLQ30_00445"
     CDS             complement(92038..>92964)
                     /gene="miaA"
                     /locus_tag="PLQ30_00445"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPW00942.1"
                     /translation="TASGKTHLLDTIFGEGADLWIPRLRSLWHADLNAAQVISADSMQ
                     AYRFLDIGTAKPSKDLLKRLPHHLIDIKNPDEQYTAGEFVERADALCNQLSAQGILPI
                     LSGGTGYYLMNFICGLPTSPPSSRAIRAQVGRDLDEFGPQVLREELAEVDPNAAARIH
                     ERDIYRLTRAIEILRTSGKPPSLFAPKKAIRSGRKFIVIGITRSREELDVRIHERVEA
                     MMKEGLASEIEKLKSLGYSSKDPGLRAIGYREFFELEEDPIERISSAIELHTRQYAKR
                     QMTFFRALPGIIWIEPYPADFYRVLKEIGSNS"
BASE COUNT        25878 a        21398 c        20014 g        25674 t
ORIGIN      
        1 cgacaaaagg tctgaaggtg gcatagacat acatctcggc aatattggcc tttaacagct
       61 gcttgctgtt tttgctaaaa ttttcagaaa ccttgtcttc ttggacaaag agcttgacga
      121 tatctattcc tgatatgtgt tccgagagat aagaatttac cttgctcgtc caatagcgtt
      181 gatttctgaa agcatctcgc gccttaatgc gtgcaattgc agcggcgata aagacaaatg
      241 gcattgtagt caatacgatt aatgctagct tccaactcaa caagaagagc actattagag
      301 accctatcat gatagaagca tctttgatga aagcgctcaa cacatcacta aagaactggc
      361 tgatgacttc tatatcgctc gttaatctcg taaccaagcg ccccacgggt tgcctcgaaa
      421 gataagaaag cgactgctcc atcacatggt ggaatagctc gaggcgcata tccttcatga
      481 tcaatgttga aatctgattc gtgaaccatg tcatagcgaa ggttgcgcca agtacgatca
      541 atagcacaac caacaaaatg aatccaaagc gtaagagtga tttatcgtct gctgctcgaa
      601 gaatcgcggc atcttcagat tttagggtcc tcaaaaccga agatggaatc actccccatt
      661 tgtctgagag ctctgctttg gggattccat ccataacaga cagcttttta tctcgcattg
      721 gatcaagagg aaatacatag ctcgcttcat tatcaaggag gccttgttcg cggagatgat
      781 ctctttcggc cattgtaagc cctgcgagat tagaggagcg cacatagatg cgtccgccaa
      841 tctcaacttc gtgagaacta tcatttccag gcagcatgga aagcgcagag ggatctaatc
      901 cataccaatc tcgaacaact gaatcatcga tggcacgcct tataattgta ggagatataa
      961 gttctccggt agtagctatt gccagggcta gaatagctat aattgcatat actccatatg
     1021 gtttgagata aagaagaatt ctgcgcgcaa tctgtgcatc atagcccttt gtgacaggct
     1081 ctgcatcaaa gtattcatcc atagattttt tcctctgaca cagaagtatc ttgccctaat
     1141 ggtttttctc ttgataatgt ttctctttga gaaatattat tcttaataga atcgatagag
     1201 acctgttgct caagttgctg caaagccgca attcttgcat agaaaccttc gctttccgcc
     1261 attagctctt catgtgaacc ctgctggatt atctctccgc tatcgagcac tatgatataa
     1321 tccgcattac gcagggtcga aattcggtgc gagacgatta tggtagtctt ggcttttctc
     1381 tcatctatga gagagctaag aatatgctct tcggtttgcg catccactgc ggataaggca
     1441 tcatctagta agagaatatt tggatcggcc gcaagggctc tggccagagc aatcctttgc
     1501 ttttggcctc cagagagtgt tattcctctt tctcccacaa ctgtctccca cccattgggg
     1561 aaaagttgat agtcatgatc gattccagaa atcttagtta tggccttaaa gcgttcgggg
     1621 ctgatattat gcgcagaaaa aaggatgtta tgctcgatgg tatctgagaa taggaagctc
     1681 tcctggggca ccattccgaa agcagatcga agctcacgta atccatatga ccatatatct
     1741 atattgccaa taaatacatg gccttctccg ggttctagca ttctaggaag gatttttaat
     1801 agtgtgctct tgccagatcc cacttttccg agaattccta acatcgagcc ttcaggaatg
     1861 tgaacagaaa tgtcctttaa ggcgggcttc tgcgagcctg gataaatata ataaagtctc
     1921 ttaatatcga tatctccgcg gggcgtcttt cttatgcaat tggatgattc cttgatttca
     1981 ggcgccgttc tgagaatttg gtttattctc ttcaagcttg cggcaccccg ttggacgata
     2041 ttcacggtaa aaccagcgct catcagcggc cataccagca tttcgagata cgaaagcata
     2101 gcgatgatcg aacctggcgt cattttgttc cgaattgaag ccctaccgcc aaagattata
     2161 aggagtatcg tgctcaatcc ggagagaaaa gtgatgaaag ggaaaaagaa gcctgaagtc
     2221 tttacgagat ccatgatggc attcttataa tcggtattgg ccattgagaa tctgttataa
     2281 aaataatcct cttttacgaa ggattttaca atgcggatgc cagaaaggga ttcctgggcg
     2341 atattcgaca agtaaccata gatatcctga atcttcttga atagtctccc gactattcgg
     2401 ccaaagagaa ggatcaaggc agtaatcaaa ggcaaaggaa ggattatcca ggcagcaacc
     2461 gcagaattgt tcaagaacat tgcagctata atcatggctg tcatgaaaat gccatcgata
     2521 agagatacaa atcccatacc ggttgcttgg cgaattgtgc ttatatcgtt ggttgccctg
     2581 gccattagat cgcctgtagt attctcccta aagaagttgc ccgacatcga caagatatgt
     2641 gagaatagcc tgtctctcat ctctgcctcg attcgtctgc ttgcaatatt gataaagtac
     2701 cgccagaaga atcgtcctat agatatcaag agcgcaagca atagcatatg aaccaaagga
     2761 tcaatgatgt caacaagagc aaaagctccg ctagaaatgc gatctatagc tatttgaata
     2821 tatcggggaa tgagaacctg gcttgcatct actacaataa ggcataagat gccggcaaca
     2881 tatcgatatc tatagcttct cagatgaggt aacagcgttg cgtattcctt aagcatatat
     2941 ctttattcta acgatggctt ggaaactcag caaggagttg cttgaatcgg agtcttctgc
     3001 catattgcat ccatgaatca gcaagaattc cttgtatata ctgatggtgg atgcaaaggt
     3061 aatcctgggc ctggtggatg ggcttatgta atgcactatg gcgaccgata tcgagaagca
     3121 tggggtggag agctttatac cacaaataat aggatggaac tcattgctgt tatcaaagcg
     3181 ctgtcctttc taaaatcaag aatagaaaat gctagttctt cggtttccga agaatcacta
     3241 gaagccccct cttggacaag agctaaaatc aatatattca ccgattcgat gtatgtaaaa
     3301 aatggtatta ctgaatggct gatagaatgg aaaaaacgcg gctggaaaac ctctgcaaaa
     3361 aagcctgtaa agaatctcga gctatggcaa gaattggata gcctatgttg cgagctaaag
     3421 cctcatttcg agtggataga aggacattca ggcaacatcg acaacgaacg ttgtgacaga
     3481 ttggtccatc tcgccataga agatgtactt tgccagaaat agagactact gctacgaacc
     3541 ttgtgtataa aaagttgaag taagaaacag gaaatagaac gaataagggg agaacaagga
     3601 gctaactatg cagactttcc ctttctgccc taatcccaaa tgcctctata atatatacaa
     3661 tcatttttaa gagaattctc aacgcaaagc agccaatagt tccttggcaa aaacttgacc
     3721 accaagtgtc ctgagtgccg aggaaattgc ctctgcctca gaaagcccca taccctgagc
     3781 gcccttgctc cccgaataga gagtatttcc ttctatcaaa tcaattgcac tcagagaacc
     3841 tgctaccgaa gcgataaaga aaccatcctc tttcccaatc gaaatcagct caacgcaagc
     3901 caaaacaaag cgcatggctt cagctggagc aatagctctt agccgatcaa tatcaggctg
     3961 tccggactct gcagagatct ttgtcgtaaa gatagagaag ccttcattta cgagtgcttc
     4021 ttcaaggctc tttgaaaacg gttcaggatc taggctaccc ttttgagctt cgctcttaag
     4081 atagactcca atcgctgtag gaacattctt agccgctgag attaccttat agctcataga
     4141 tgcccttatg cccgacaatt cgctttctaa tgcatctatg tagggcaatg cccattttgg
     4201 taatcctccc agaatatctg caatgctacc aaaatccaac tgagcagtca cttgatactt
     4261 gccagctaat tctattggag ggacatcgaa tgaaacgatg ccgtcttggt tggtgaatac
     4321 ttgttgtgta gcggtaccta ttcggcctga agtcagtctt cgcggataag ttatagcaat
     4381 cggagcaccc gggactcctt cttctcttcc tccgcgatta gctatgagtt tcaccgtaat
     4441 aaatggaacc tttgcaccgc ccagctgaat ttcaatattc gatggagata atattgccaa
     4501 tctgagagtg gcggctaatt cggaagcttt ctttgcattt cgagcgagct ttatctgtgc
     4561 attctctacc tcagagcttg cagccgccga catggcttct gcataagcgc gaatagcatc
     4621 tatcaagagc ccctcattgg ctgcggcatc acccttctgt tcaggtatag caaccgcatc
     4681 ggctttttcc ttgagtaatg attccatccg agctttctct ttatttagct cctttgtttc
     4741 atatcgagcg agtacatgca ctgtatataa cccacttttt ttatcctgtt ctataaattt
     4801 ttcgaccacc tcaaaacccg aaagccgacc tttagcctct gttttgactg tctgtgttat
     4861 ttgggcttca tattcactga gagaagctct tgcttcggcg ctggaagcca cgcttatcga
     4921 tactcccatg tattgcataa tacccgcaat taaagccgcg gttgcatcat ttagggcagt
     4981 cgcgctatcc tttgccgaag aagaacctac aaaataagta tattttgcat cagggcttgg
     5041 cgttttcttt acccattctg gaatgcttgg cacactagcg caactagtca caagcccgaa
     5101 aagaacaacc agacatataa tgatgctctt tctcatttat cattcctctc tgggtaagaa
     5161 agcctacgat atatttagga gaacctcggc atcatatctt ataccatgca ccgaggcctc
     5221 cccatgaaag gattttctca tcaatcagat tcgccaaaga aatctttggt tactgtgttc
     5281 tgaactagat ccattgcgcg cttctgctca ggcgttggag gctctccccc tgccatctga
     5341 gagaatagca tatcaaactg tttttgaagc atattcttat cgatcgccca tagctgaatt
     5401 acccgataaa tttgcttgtc aggcttattt tccggagtat aggtctggac cttaacccac
     5461 caatcagcct ccatcctaaa gccagagaat gtagcttctc ccacactcat gacaaaacgc
     5521 tcgccatatg cgccaaaatt cttggaatcc gcatctgcta cgtttgcacc tttgaatgca
     5581 tctttcactc gcgtcgataa atagcgagca atctcggtat cagcctgcat tcgctccgcg
     5641 agcaattgtg cactctggag attctgagct tcaaattgag ctataaaaac cactttatct
     5701 ttataatcgg gtagtttttc aatatctctg tagccaccta taattgcaag ctctacccat
     5761 tttggtgttt ttatgccata tgcagtgcct ttatcctcaa tcagcacttc accctttcta
     5821 agggggggct tagtaccgca tgagatgatc actgcagcga ctagcaaaac caataccgcc
     5881 tttcttgctt tcatcttttc ctcctaataa tgaacaacat tatgaaaatg cactaaattc
     5941 ccgtattaaa tgtgtatgat ctgcctctca tgtatagaag atagcaatta gggaaaccgg
     6001 acatcgaagc cgcatcatat atagcatctt gaatcttcga agctggcata aaagcatata
     6061 tagcaaatct ggtttcaccg ggtgaatagg agagtctttc aacctcacgt accctttttg
     6121 ccaaattcct ttcgaattga gatattgcta tcgcatcagg agtgttctgt agtatgagtt
     6181 cgtatcgaac tcctcgagat gcggtcattg tgctcataag atgctttgct tgctccgtga
     6241 cctttggcat cgattgccag ataattcctg atatagcatt catcattgca gcatctgcgg
     6301 agacagggct gaatgtctgc gggctcaaaa aggatatcga tccaagaagc gccgcagtag
     6361 atgcatcgaa aatctttata ctgccttgtg ctgatccggt ccaggctcca ggccctcctc
     6421 caaagcttgt cttagcatct atttcgaggt agatatctgc attgagcttc tgtgcaatat
     6481 aagtgatgat atcgatagag ccacctgtct cagactgata agcgcttatc tgatctttct
     6541 tattcttctg gatctgatcg aaatcgatga cagaaagccc ttctttttcg attaggtatc
     6601 gatttgcctg tgatacagca gcttttgcaa gctgtgcatc caagccactg ttctcgttgt
     6661 aatacactaa gtagcttagt ctgttaatat aattctgaaa aaatctgctt tcatcggcag
     6721 tcatcggttg agtataataa cttgcgcttg cctgtgggac agaagaaggc tccgatggag
     6781 ccggagtctc accaggcata acttggcttt ctacaagcgc ttctgcggag gattgaacat
     6841 ctccgggagc cgttgtgccg ggcatttctg gtgtcgaagt gccctgaaca atctcttttg
     6901 ctctctggct ttccagactg ggtgcttctg gctgagaagt tgttgcgcaa ccgataagta
     6961 gaccgataag cagcagaaca cccataaaac atttataaaa ggaccatgct cttttcatat
     7021 tttcattatg agatagcatt tatacttttg caagatgaaa atgctaaatg ggacaaaagt
     7081 acgaaaggtt tttctctgag cgatgcgcga tttgaacgcg cgaccttcgg ctccggaggc
     7141 cgacgctcta tccagctgag ctaatcgctc ttttttgctt ggtcttgcct gcattttcta
     7201 tatttttgca tattaagact tcgatcgaaa cgcgtcaagc caaggctcat tttgaaagaa
     7261 catattcgaa aatctatcaa ggtcttgtca aagcatccat ctctcattag attgcttttt
     7321 gtcatgtatg gagaacctat tctgcttgca agtgaatcct cgcgaagggc ttctattctt
     7381 gaatcgatca gaataccttt tatcaaagtt ctaccttcca tcgatgagac aatatttgac
     7441 aacctagatc ccgcagaacg tgttgtagct cttgcagcaa caaaagccaa gcatggcaaa
     7501 gctctttggt ccgagaaaaa tcgaagcaaa aaggtagtta tatcgtcgtc tgccaagaag
     7561 cctttagata aaaatgctct agatcaaaaa agcaacgcag aacctcgata tgtgcttggt
     7621 gccgatacac ttgtggcctt taagatatcc gggcgttgga ttacgctcgg aaaaccggct
     7681 aacgaactcg aggctttcga aatgctttcg atggaagcag gcaaacgaca gactgtattt
     7741 agtggacttt gcctgctcga catggtaagc gatcgatgct accccgctct ctctatctct
     7801 gaagtccagt ttgcacccat gacacctgaa gaagtctcat ggtaccttag cttcggcgaa
     7861 tggcgcggag ccgcaggagc ataccgaata caagaaatcg gatcatgctt catagaagaa
     7921 ataagaggtt catcctcggg cgtaatgggc ttgccaattc atgaattata tggcattctc
     7981 catgcgtcgg gatacttgga ttcttagcaa gaagagattt aaatcaccga taaatccagc
     8041 cttttgagtg ctgggcattt tccgcctgac tttccgagag agcaggacga gatataagga
     8101 agggaaccgg aatccattcc ggtaagggga ggtctacgtg gcagtagtga ccatgaaaaa
     8161 tctgcttgaa tccggtgtgc actttgggca ccaggtcaag cgttgggatc cgcgaatgaa
     8221 aaaatacata ttcgcggagc gaaacggtat ccatatcata gatctgcaaa aaactattca
     8281 agccatcaaa gaagcctatg acgcagtgca gaaggtagcc agttccggga aatctgtgct
     8341 cttcgtggga acaaagaaac aagcccaagc cgcaatacaa aaagaggccg agcgctgcga
     8401 gcaattctat gtaaacaatc gttggctggg tggtatgtta acaaactttt ccaccataag
     8461 aaaatctgtt caaaggctca aaaagataga gcgcatggaa gtggacggaa ctttcagcag
     8521 tcttaccaaa aaagaaatcg caagtcttct aaaggaaaag accaagcttg agaaaaacct
     8581 cggaggaata aaggagatga cgagccttcc gggcatcctt tttgttgtgg atacgcgcaa
     8641 ggaagcaatc gcggttgctg aggctcaacg cctcggtatc cctatcgtag caatcgtcga
     8701 caccaattgt aatccggaag gcattaccta tcccattcca ggcaatgatg acgcaattcg
     8761 atctatcagc cttttcactc agatcatagc aaatgctgta atcgaggccg gcgcgtccga
     8821 aggcttgaag ataatcgaga ccctgccttc tgaagaggag caagaactga tagttcctca
     8881 aaaactagag gaagatgagg tcgagataga tgtagagaat tatgccgaaa ccccaagtca
     8941 gagtaacaaa gaagcggatg aacagcagga agtcgaacaa cccataattg atgaagatcg
     9001 tctttatgat gaagattgag aacattgagg agaatagatc gtggaaatca aagcgaatga
     9061 agtaaaggct ctgcgcgaaa aaaccggcgc aggcatgatg gattgcaaaa aggcactaat
     9121 tgaagctaat ggcgatttct ctgcagcgga aaaattattg cgcgaatggg gaattgccgg
     9181 tatcgagaaa cgagcaggtc gtgcaacaaa tgaagggcgt atcttcatag ctcaaaatga
     9241 taaggaatta gaactgatag agctggcttg cgagacggat tttgtggcta gaaatgagga
     9301 ttttgtggcg gcaggcacta gaatcgcaga aagcgctctg tcacagaagg catccagtac
     9361 aaacgaaagc ctagaggcca ttgtaaagga aattgcttcg cttataaagg aaaacattgc
     9421 gctgagacgc gttgttcatt tttatgccga agaaaacgaa attctgcacc cctatctgca
     9481 tggcgaaggc agaattggag ttgtcgtaaa agttagagcg aacgaccctg aggctttcaa
     9541 gcatgagaag atcgctgcct ttatacatga tatagcttta catgtggcag ccttcaaccc
     9601 aatgttcctc gatgaatcaa aagtctcggc tgagtggatc accgaacaga aagaaatctt
     9661 ccaaaaacag ctcgagctcg acgaaaagct gaagagcaaa ccagaaaaag tccttgaggg
     9721 cattttggct ggaaagctaa agaagcttct tgcagatgtt tgccttctaa accagggctt
     9781 tgtgcgagac gagaagtcat ctgtcgcctc tattctagcg cagatttcta aagagactgg
     9841 ttttcagctc gaaatcgtag attattattt tgcaaaagtc ggacaagatt gaatatgctc
     9901 tgctaatatc gggtaaagac gggctttcta ttcagaatgc ctctctgcac ctgcaaaaca
     9961 gcatgtcaag gaaggattta tggaatcgaa caaagctgcg catgaagaaa agatgaagaa
    10021 gactatcgct gcatttcaag atgagctgaa tagcatacgt acaagcagag catcccctgt
    10081 tcttctcgac aagattagag ttgactacta tggtcaaaaa acgcctctat cccaggttgc
    10141 gacaatttct gttcccgaag ctcgtctcat cgttattcag ccatgggatc gctctttatt
    10201 cagcgaaatc gaaaaggcaa tcctcaagtc agatctaggc ttaaatccct ctaatgacgg
    10261 aaaagtgctt aggatagcta tccctcctct aactgagcaa cgaagaaaag aacttgttaa
    10321 aacggctcga agtatcgccg aacaagcacg cgtggcaatc agaaatataa gacgcgatgc
    10381 tctggaagag cttaaaaagc agcaaggcgc aggaggtgtt gcagaagacg tccttaaaaa
    10441 agaagaagca gagctccaaa agatgacaga tgattacatt tcccaaatag ccagaattct
    10501 cgaaataaaa gaaagagaaa ttatggaaat ctaaggattc ttcaaaatga aagagaaccg
    10561 catgccttct catgttgcga tcattatgga tggtaatggt cgatgggcaa gacagagagg
    10621 ccttgctcga acagaagggc acaaggaagg attaaagaca gcaaagcgta tagtgctggc
    10681 ttctcggaaa gaaaaaattg aatttctctc gctattcgtt ttttccacag aaaattggaa
    10741 gcgtacagaa caggaagtcg gatttcttat gtctcttatt cgagctcata tcacaaagga
    10801 gttgaatttc tacagaaaaa acgagatacg catagtccat tcaggcgaac gaaatggact
    10861 accgcatgat gtactttcag aaatcgataa ggcgattgag aatacctctc agtacagctc
    10921 attgacagtc aacttggcaa taaactacgg aggcagagac gaaattgtcc gagccatgca
    10981 gcgaattctg gcaagtcaag aaaatgatga tgaaaatcca atagatgaat tgaaaattat
    11041 gcgatacctc gatcatccgg aaatacctga tcccgatctc atcatcagaa caggtggtga
    11101 gatcaggtta tccaattttc tattatggca atctgcctat agcgaactat acttttcgcc
    11161 aaaatattgg cctgatttta ccccggagga tcttcacgca gctcttatcg actatgtgca
    11221 ccgcgaaagg cgattcggag atgcacggtg aatgagaata tgagggcaag actcctcctg
    11281 ttttttgtag gttttccttt attaatcctt tcgctttttt ttcttcaatt tgcgaattat
    11341 atagttattt cgggaattct cctcattgtg caattccttt catccaaaga attgcgaaac
    11401 atgctgacca gagccagttt gccggacaca ggagacttag caactttcat tggattaatt
    11461 ccatcagtct tgacatttat atcgtgtata ctatatggat ctctcctgga gaccgcattt
    11521 gtcttattta tttcctggtc attttgctta atgcttctgt taatcccgct tgtattccaa
    11581 aaaaagaatt ctttcccaga tcttatcaag caagcagcat caatcgcgct tgtccattgt
    11641 tatacagcgc ttcttccggg atttctcata ttcattgttt ccggatttcc taattcgcag
    11701 aatacgataa tcagctttgc tttgcttaca tttggaaacg acagccttgc atggtttttt
    11761 ggaaaatacc ttgctaggaa aaggaatcta ctagatgtat caccaaacaa gagcatttcg
    11821 ggttttatag gaggtacgat tggatcgata gtcgctgcct ttattggact gggacctatt
    11881 gccggcgctt ggaagccttc gggaattgca tatatattac taagcctttc tcttggattg
    11941 ggaatggcat tctttgttat tgtgggagac ctgtttgaaa gcgctctcaa gagatcggca
    12001 ggaattaagg attcaggctc tatcgtacct ggcaggggag gaattctgga ttcatttgat
    12061 agtctatatt ttagtgcacc cttttttatt gcattttcgt atacttttca tttgttcaat
    12121 ctctagaagc aagggtatcg gaggtaaaag ctgaagcgtg ttatcgtact cggagcttct
    12181 ggctctattg gaagacaggc tctcgatgtt attcaacgga gcgcggattc ctgcaacaag
    12241 ctcgagcttg ttggtttttc catccatagc aatcgcatcc tcgccaaaaa gctcaaaagg
    12301 caatttcctc aagccaaagg agcttggacc ggcaatccat ctcttgctcc agaagagatg
    12361 gattggaatg gcctcgacgc aatatcgaac cttcttttgg aaaccgaagc agagatagtc
    12421 ctaaacggaa tcgctggagc tgctggtcta tcggcgtcga ttctagcatt acagaacaaa
    12481 agagacctag ccctggcaaa taaggaatcc atcgtcatgg gctatagact tctaaggaat
    12541 cttgctcaaa aaaatcaatg ccaaataata ccagtagatt ctgagcatgc tgggattttc
    12601 caaatagtcc agagatttgg cagagacctt gtctccgaac tcataataac cgcatcaggc
    12661 ggaccattct atgctcttcc tcttgaattg ctcgatgctg ttacggcaga cgaggcttgc
    12721 aagcaccctg tatggcaaat gggacgaaag atcagcattg actctgcaac aatggctaat
    12781 aaagggctgg aactaatcga ggcttccagg ctttttgata tgcctcaaga gcgaatcaag
    12841 gtgcttattc atccgcagag ctatgtccat gcaatggttc gtactatcga gggggttctc
    12901 tacgcccaaa tctcaaaacc tgatatgcgt ctgccgatac aaatggcgct gtattggcct
    12961 gagattgtag agacttcctt cgggtttgca gatttagcag acataagcct cgatttctat
    13021 gaacctaagc aagaacgata tcctatgctc tggattgcac gccaggctat cgacgaggga
    13081 gatgctccat gcattgtgtt caatgcggcc aatgaaatag cggtagaatg cttcgataat
    13141 ggaagaatca gatttaccga tatatcgaac ctggtcgcaa aggtactcga tcgagattgg
    13201 aatttgccta tcgaatcatt cgaggatata ttcgatatag atatcaaagc gcgcagaatt
    13261 gccgctcatc tctcggagag ttgtggatga tcagtattat tctaggcctt attggccttt
    13321 ccatagtcgt gatatttcat gaatttggcc actttgtctt tgctcgcttg tctggcgtag
    13381 aagtagaggc tttctctgta ggttggggac catcaataat ctcccacaag ggcaaaaaga
    13441 cggtatggaa gctttgtgct tttcccatag gaggatattg caagttaaag ggagaagagg
    13501 gatttcaagc agcggtggat caaaatctcc aatatatccc ttcggaaaaa ggtagttttt
    13561 tttcggctac tccattaaag cgcatcttca ttgcttctgc cgggcctctc ttcagcgtac
    13621 tttttgctgc atttatcttc atattggtag catctatagg gactaccatt gtaacggcac
    13681 caaataggat cattctttcg agcgaaattg ggtccactct tcaaagcgga atgcccaatc
    13741 ctgcggatat tgcaggcctt aagacaggcg ataagattat atcgatcgat ggcaaaacaa
    13801 taagggatta tagcgatctc caggagatca ttgccagcaa tcctggaaaa accatgtcca
    13861 ttgaggtctt aagaaatgac aaaaaacatt cgctcgtcat aactccccta ctggatccaa
    13921 attcgggtat aggcgtaatt ggcgtatatg cctggattga tcctattata gaatcagtgc
    13981 aagagaagag tcccgcggca attgcggatc ttcgtccagg tgacatcatt atggaagcgg
    14041 atggaagacc catttcgaac accgtagatc ttatgcaagt attcaacaat gcaaatggtt
    14101 ctgtgagtct tagagtaaag cgcggagatc agactctctc aatgataatt gtcgcgaaat
    14161 cgcttgaaga agcgggcatt agatttgaag gtatcttgcg aacagaaaaa gctaagtcgc
    14221 tcccagaggc tatttcgatg ggggtggaag agactttttc tacaatttca ttggaaataa
    14281 agagtatagg catgcttttt cgcggtgtaa atgtcttcaa agccatttct ggaccagcca
    14341 ggttgaccta tctaattggt accgctgcct ccgaacagat aaaagctgaa ggcctcgccg
    14401 ggctggtacc aattctcgat ttcctagctt tcatatcgat agcgcttgcc attatgaatc
    14461 ttcttccaat tcctgcttta gatggtggtc gtgtccttat ttatttgata gagcttatca
    14521 gaaggcgtcc aattaatgcc aagtccctct atagatttca attcattggg gtcgcttcta
    14581 ttctcttgct attccttgta gcaactatga gtgatatttt cttcttttta ggtaaatagg
    14641 gatgcaactc atgaagatta tgtgcgatct ctacgctcca tgttcacaaa tttcgcatag
    14701 ctactcttga accaaaccgg agccttacct gttggcccat tgcgctgctt ggcaacaatg
    14761 atatcggttt cgatttgatc ggtctgtgag tcatatgtct tggaagtttc tctatctcta
    14821 tgaaggaaga ttataagatc ggcatcttgt tcgatcgacc cggattctcg caaatcagca
    14881 agagtcggct gtttgccctc tgcttcgcgc ttgagttgcg aaagagccac gattggaata
    14941 tctagttctc tggcgagagc cttgagcgat cgcgagatag ccgctatctg ttcccaccgg
    15001 ggcaaatcgg cgttttcgtg ggttattagc gtaagataat ctacgaatat tgctcttacc
    15061 cctcgttcta gcacaagcct gcgcgccatg gtcctgagct caaggagttt catgtttggc
    15121 agatctacta tgtaaagtgg agcttcatat attcttgagg ctgcatccat aaggctatca
    15181 taatcggtgg tctttattcg acctgtcctt atgcgatcgg caggaatcct tgcttcagaa
    15241 gcaataagcc tcaacataat tgctgattcc gacatctcca aagaaaaaaa tgcaactgga
    15301 atctttagat tgattgaggc atgcgctgcc attgtaagag ccagggcagt ctttcccacc
    15361 gaaggcctag cccctatgac tatgaattca gacttctgaa gaccattcgt taatgcatcg
    15421 agatcggaga ggccggaagg tataccggta aatgcagctg ggtccgtact gagtttctct
    15481 attagcagca tcgtttctgt tacgatctct ttggcagagt gatactcagc agtccttcga
    15541 ttctgcgata tctcgaatat cttagcctgt atttcatcga gaatcgcatc gatatccttg
    15601 gtttcttcat gcgccatggc tgcggtttca gcggataggt gcactagtcg ccgtcttgtg
    15661 gccatttctt gcacaatttt tgcatagtat tcgatatttg cagcagaagg agtaaatgaa
    15721 gccagacttg caatatatgc acttccaccg atcctttcaa gattgccgct gcttcgcagc
    15781 tcctctgaaa gtgtaatcag gtctggcttc tgcccttttt cgtgcatagc gagaagagct
    15841 tcaaatactt cctggttggc tgaatcatag aagctgtcag gacgcaggta tcttataaca
    15901 ttgtttatcg actcggggtc tagaagaaga gcgcctaagc atgcctgctc agcgtcacca
    15961 ttataaagcg gtaatgtgtc tttaagcgat ggacctgcca tctaaaaatc ctctagatgt
    16021 accctaccct tttagagggt taaatactag atcagctcaa ctgaccgcga ttcacggctg
    16081 cctcaaatgc tgcagcttgt tcttcaggtg ttgctggctc agattctgtg cgttgcctgc
    16141 gggttctgcg cttcttgcct gcttcttcgg aaggtttctt ctcctctgca ctcgctgatg
    16201 cttgagcctc tatattacaa tgaagtatag cttcgtcttt ttcataaagc ttaatcacga
    16261 tcttatagtt gccgacagat tttatcgaac gatctggcac ttcgattctc tttctatcga
    16321 cttctatatt tttcttcaaa agttcttctg caattgtgtg gctcgtaacc gcaccgtaaa
    16381 gttttccatt ctttcccgca ggcatcgaga tgacgagagg ttctgcctcg attctctctt
    16441 ttaaagtgcg agaagaaagc cttttctgct ctttaatggt ttgaatctcc gcttgtcgct
    16501 ttagaaacaa tgcttcggtc ttttcattgt agaccatgac catttttttt ggcagaagat
    16561 aattgcgtgc atatcctggc gcaacttcct tgacgtctcc aatttcaccc aaattgggta
    16621 tgtcttgatt taatataact ttcatgttca agctccttta tgcattcgat attgcagcca
    16681 aacttccgat acaccgagca ttggggccag gattgcagct gcaatcctga ttttggcttc
    16741 gtaaaacatg agtatcatga gcaaaatcaa taataggcca gctaggcgct gcatatttct
    16801 caatcgaaaa aaatacgaaa tcaggctaaa gccttgtaat gcataccaac aagcagcggc
    16861 cagagagaca ttccaggcga ttatggcaaa gactcctctc tcatttccat aaagaacgca
    16921 gattagagca agccaaccca aaatgcttgg ccagagaagc catgaaggca cgctgaatac
    16981 aaatgcattc ttctttgctt ctcctttttg cttccaaatt ctagcagatg caagactatt
    17041 gcctatatgc cagctacctg caagcagaag ccacgatgct actccgaaag atctcataat
    17101 gatcgatatt gcaggaatga cataattctg ttcgatagcc gatgcgtcca aatcctgaat
    17161 gcctgatgag ttcaaaagct gggctatcat atctgctatc gatctccgtg cttctggtgt
    17221 acctatacta atactgaaaa gataagcaaa tagcacagag acaactattg tagcagctat
    17281 gatttttacc caggcatcgc tctttaatag attcattgct cctattgttg ctagaagcaa
    17341 tgctggaagc aatagagtct gaagaaatat gggtaactct ttccagccca tcctagaaag
    17401 ttcaattgtg cttattgccg ccactatcga gagtgatact agaatggaga gcaaaccttc
    17461 cctcttgttc tccttacgaa acacatattg aatagggatc aaaaatgcaa acgcaagaaa
    17521 accgctagca tagagaaatc cagacagaag tccatatgga ataaagacac gccatgaata
    17581 tttgctagac gattccgact ttatgctctg agatacttcc atcaatgtcg ctcgctcttt
    17641 ggaccagatt ccaaacgctg ggctatttta taacataggg aagaagccca agagcccttg
    17701 ccctcttgat atttactgcc agcattcgct ggtgctttga gcaagtacca gtgatgcgcc
    17761 taggaagaat cttgcctcgt tcagtcgtat aacgccgaag aagctctgcg tctttataat
    17821 ctatctttac attctgagtg cagaagcgac agactttttt acgaaagaag ccctttcctt
    17881 tgcgaactcc attccggtca tcctgatctc tgtgcagttc ttgttgttcc gcttctacat
    17941 tagaagctct agattcatcg atatctctca tctcatccat actaatctcc tatcaaaatg
    18001 gaatatcatc agtaaactcg tcgattgtcg gaggaatagg catctgatta ttggcagaat
    18061 caatctttga tggaatatta tctgctgcat atcttctgcc acccatttcg ccactatttt
    18121 gagacggggc tcccaatggt cgaatagttc cagcgacaat aactactttt gagcgctgtt
    18181 ggccatcttt ttcccaccta tcttggcgaa gttctccctg aacggcgacc tgtcggcctt
    18241 tgaaaaggta tttgcttagc ccttctgcgc ttttcccata aaactcgata tcaaaataat
    18301 gcggctcatc aatccattgg tcatcctgct tcttagagcg attcactgca atggaaaaac
    18361 ggcatattgg aagcccagac ggcgtgtatt tcaattcact gtcgcgggta agacgcccaa
    18421 ccaacactac tacattgata tcactcacaa tatactcctt tagtccttgt gggacttggc
    18481 cttattcttc aacctttacg accaggtgtt tcaataccaa agggtttagc tttattgcgc
    18541 gctcgagcga aacaatggaa gcaggatcaa aactcattcg atagagcata tagtgagcgc
    18601 gtgtcttttt cttgattgga tatgcgagag gcttgtctcc catatcttct tcttttacat
    18661 caattgcctt atactgcgaa agcatatcgg cgattgcctg tttgccggcc ttgaattctt
    18721 cttcttccga gctcagcaca gtgactagtt catactgtct cattcaatcc tccctgtgga
    18781 catatgcact catacgagtg cggatgatcc ggctgataga accggataaa gagggcctta
    18841 tttatatatc acaaatgaat gcgaaggaca agcatagacc atttgcatat gtctcttcta
    18901 agtataacta gccctcttgc atccattgct gattattctc ataattactc aacttatctt
    18961 tgcaatactg cttaattcta tattgatgga gctaatgaat gactgttatt aagccgagag
    19021 tgctaaaagg ttttcgagat tttttacctg atgcagagat aactcgctct tctctggtaa
    19081 gagaacttga gcaagtcttt gcattatttg gttttgtacc catagacact cccgtcctcg
    19141 agtatgctga catcttgctt ggaaaaggag gcggagaaac agacaagcaa gtatatcgct
    19201 tccttgatca tggcgatcga gacgtagcca tgcgtttcga tttgacagtg cctttcgcac
    19261 gatttatggc agaacatgtc gacgagttat atttgccctt taggagatat cacatagcaa
    19321 aagtatggcg cggagagaat actcagcgcg gaagatatcg ggaattcatg cagtgcgatt
    19381 ttgatatcgt gggtactgat tcaggctctg cggatgcaga tattctgctt acagcagcca
    19441 aagcgatgct aagactcgac gtcggagaat tctcgatccg cattaatcac cgcgcacttt
    19501 ttaatagatt cttggaacat attggcgctt cagccagatc tacggaaatt ctcaggaccg
    19561 tagacaagct agaaaaaata ggttttgaga atgccttcga aaagctctcg aagttgctgg
    19621 gtatgcaatt ggcagacgat attctctctt ttatcaaaaa agaagagagc tttgaagcaa
    19681 ctcttgaaaa gatgattggt ttttgcgata aaaatgattt tcaggcgcaa tcggcaaggg
    19741 atcgtttgct ggaaataatg gaatatgtgg ctgcagcagg ctttgagaat cggattatgc
    19801 ttgatccaag catcactcga gggctggatt attatacagg aatggttgta gagacatcgc
    19861 taggagctct gcctgagatt ggctccgtat gctcaggagg cagatatgac gaactcgcaa
    19921 gcctatatac cgagagaaga atgccgggag ttggagcttc aattggtttg gatagactta
    19981 tcgcagcttt agagacaatc ggtaaaatag acaagtcaat aatatcgacc caaatactga
    20041 taatgaatca ggaaaaaggg aacgtaggta aagtcgcgac atcagagctt catgcgctag
    20101 cggctcgtct tagagatgcc ggtttttcct gtgaagtatt tcctgaggag aaaaagaccc
    20161 ttgcccagta tacctatgcc gagcgaaaac acattcctct tgctctcttc accaattcgg
    20221 caaaaggccc ctataatatc aggattctag cggagagaag gaacatagag tgtctggatt
    20281 tcgacagcct ctgcaaattt attaggagct tcctgaatgt ccaaacctga aatggaagaa
    20341 ggacaagaca gaaaacctga gcaaacccaa tcgatgagcc aagaaattgg gcttctggac
    20401 ggaagcattc gttcctttgt gaaaagatcc ggaagaatta ccgaagctca acgcagagcg
    20461 ataaagttat taggacccaa atacataatt ccattttcgg aagaattact gattctggaa
    20521 gaattatttc ctcagaatag accaataatc ttggagattg gttttggatc cggacaagcg
    20581 acatggcgaa ttgctcaaga aagacctgaa ttcaactatc ttggaattga ggtttatgcg
    20641 tccggcgtag gaaggctaat aatggacctc gaactccatg ccatagagaa tgtgcgcata
    20701 ttgcagcaag atgctatcgc tgtgttgagg cacatgatag cgcctagaag ccttgccggc
    20761 atccatgttt tttatccgga tccctggcca aaaaaacgac accacaaacg caggctcatg
    20821 caatatccaa tcgtctttct catggctcaa aaacttatgc ctggaggcta tctctattgt
    20881 gtaactgata tagaggaata tgctagcttt gctaaagcct cgcttgacgc ctgctcactg
    20941 ctagaaaacc aatatgaggg cttcgcacct tttcaaccat ggcgtccgga aactagattt
    21001 gaaaaccttg caaaagaggc tgggaatcaa catttcgagc tcttttacag gaagaactag
    21061 cggtctagat catgcttttt gaaaacatgg cctgatttgg tctctttcgg caaatgccct
    21121 ctgtcggaga tgccttcttt cggctctctg ttttctctgc cttttgatat tgagacaggt
    21181 tccttctgct ttaatgcaat gatctgttct gctagttccg gcgatatttt atatatctga
    21241 gaccttgaca gtggagaaac agacatcgca tacattctgg tagtacggac aatctcgcct
    21301 gcaactataa aatctgcatc ctgtcgaaac ataaccgagc ccggatgaat atggattttt
    21361 tctgcggtga gagatctaaa tatgcctctt ccttgtcgtg cacatacaaa ttgaataagt
    21421 cctcgactaa cggcacatag ataatcgtta atagaaccac ctgatccaat aggaatgccc
    21481 atatcagata caattaggtc aagctgctcc ttgattctaa ggatttctcg aagcgtgcgc
    21541 tcatctaaat agttgcgctc acagaatttt gtcttgtccc gagcagaaga gaaagcgtca
    21601 tagagcttta gatatgagac aaagtcgccc atagcatcta aaaagtgatg atgagcagat
    21661 cgcgcctctg tttcttcacc tggaggaaga atatatgggc ttgtagtcga tagaaaagcc
    21721 gctgcaataa gcgtctggct caaaacacct ggatacttaa ggatagcctc aacaatcatc
    21781 cgcgatagct ttggcagaag tggaaaggca cacatcatct cgccaatatg tgtaagagat
    21841 ctgtctgtat ttagtgcatc gagcatgttc agtgcttcga tggctcctcg aattgcgctc
    21901 tttggcggct ttgttatgaa gtcgaaatgc tcaaaatcag ttatgcccag ttcagccatt
    21961 cgcagcagaa cctccgatag atccgtcctt ctgatctctt ctgtggaata gaggggacgc
    22021 gattcaaaat ctttaagaga atacagacgg tagcagattc cgggacgtgt gcgccctgct
    22081 ctgcctttgc gctgattaga ggaagctttt gaaataggag tctcaattag gcttgcagta
    22141 aaggttttag gattataata attgattttg gccaatccag agtcgacaac aacagttatt
    22201 ccatcaatgg tcacgctcgt ttctgcaatg ttggtagcta ttaccacctt gaccttacct
    22261 ggtggaggac tctcaaagac tctttcctgt tcatccttgc ctaggcgtgc atatagcgga
    22321 atacaatcga gcttagaagc ccagcggctt gtcatcagaa cctgtaggca ttccttgatt
    22381 gtcttttctc caggaaggaa aatcaggatg tctccagatt cggcatctgt ttcccctctc
    22441 agtactctat ctatgatgga aaggatcttt tcatagagag cctcatccga agatcgataa
    22501 tataactcat tgctttcttt ggcgttggga atatatcttg atcctgtttc tgatacaggc
    22561 cgaattgcat catagatgat cctgaccgga aacataggag catcgattct tacgactggg
    22621 cattctccaa aatattcgga aaaaacttcc gcatttattg tggctgatga tacgattacc
    22681 ctaaaatcat ctctagcatc tatgactttc ttaagaagcc ctagtataaa gtcgatattc
    22741 aggcttcgtt catgggcttc atcgaccatt attatgctgt aagaagaaag atacgggtca
    22801 aatttcattt cctgcaagag aataccatct gtcattatct ttatggctgt attcggggct
    22861 gtcacatctt caaaacgcat cttgtatcca acgattcccg gcacagtggt attgagttga
    22921 cgggcgataa aatcgcttac tgacaaagtc gcaattcgcc gtggttgggt tatgcctatt
    22981 gtgccatctg ctgcatatcc agcctcatag agtataatag gtagctgtgt tgtcttaccc
    23041 gaaccagtgg gactctctac gacaataacc tggttctttg ctagagcttc taagattcga
    23101 gatttttctt gatatattgg aagatcatga ggattcatca tgactctaag tttaatgata
    23161 tgaagagaat tcagccagta tgcgacattc acccaagaga aacattctga taaaatccga
    23221 gtatgaactt cgattccaat cttgatgccg aaagccgtct attctctccg ctttctgtct
    23281 cccaaatcac ttctcttata agaaataact tagagaacaa attcccagat gtcatcatcg
    23341 agggagaagt ttccaactgc aagatggcag cttccggaca tctctatttt tctctcaagg
    23401 acgaggaagc cattcttcaa gttgtcatgt ttcgaaggga tttgcttacc cttgattttg
    23461 tccccaaaga cggtatgcaa gtatgggcac gcggagctat atcagtctat gcgcagagag
    23521 gacaatatca gctcattgca aggaatgtga ggcagacagg atttggtgat atcttggcca
    23581 tgctggaaca gcgcagacga aaattcgcgg aggaaggcct gttcgatgaa tccagaaaaa
    23641 aagaaatccc tgcctttccc accagattag gagttgtgac aagtcctaca ggcgccgcga
    23701 tacgcgatat aattcgtgtg attcaaaggc gcaatcctaa ggtgagtatc ttgatccttc
    23761 caacattggt gcagggacaa gaagcagccg cttcaatcgc ggagcgaatt cgacaggcaa
    23821 acctctggaa tctggccgat gtgctcattg ttggacgagg gggaggttct atcgaagatt
    23881 tgctgccctt ttcggaagaa gtagtaattc acgcaattgt agaatccaac attccaataa
    23941 taagtgcagt aggccatgag acagactggg ctctttccga ttatgcggca gatctgcggg
    24001 ctccgacacc ttcggccgcg gccgaaatgg catgcgctga tttatattcg atatataagg
    24061 agatagctca ttttgaaaat atcatggggc aatcgatgaa gcactccatc accaatgcga
    24121 agcaaagact ctcttcgatt tctcctcata ttatggaaga catcctcctg cgaaagaatc
    24181 tggtagcaag ccagagattt gattcggcta tcgaaagcat aaggaacagt tttcagaaaa
    24241 aagcagaaga cctgaatcac cgcttggtcc ttgcttcgag cgctatagaa atagccaacc
    24301 ctagggaaat aatgaaacgt ggtttctcag ttgtcaaaaa gaacggcgtt gatatagtac
    24361 actcttccag aatgctggct cttggcgatt ctataaacat catgttctat gaaggtgaag
    24421 cggatgctga tgtaaaagcg accagagatt atggtgacaa aaggagttca ggatgaaaga
    24481 tttcgaaaaa aggattgcac atctcgagga attagcggag aaaatgagag atccggatct
    24541 tcctttggaa aaatcgtttg gctttttcga agaaggtgta atgctcgctc gagaacttag
    24601 gaaggaacta gatgagctac agggcaaggt agaaatcctc ctcaatagcc ttgaggtcag
    24661 agaaaacgat gaggctgaag tgcccaacat taccaaattc gattttcagt cttccgagtc
    24721 tcaaaatgac tctcaagaca agcaagcgcc ataatagacc tcaactacct ggtttgacgg
    24781 ccgcttccaa aatgggaatc agggaacggt cggagtcggc gagttccata ttcgaaatcg
    24841 attctatagg aagccatgct acttcgacat gctcattgag ttgtcggatg ctcccagggc
    24901 taaaagagac ctcccaggcg gctagagtcc gagttcttcc cttattctcg aaaatgctct
    24961 cgcctaaaaa ccttattaca tcgatattag cgccgaattc ctcgaggaat tcgcgcttga
    25021 gagcagattc atccgattcg ccttcttcca ctttgccgcc tggaaactcc cagcggtgac
    25081 tcatttctgt atgatccttc ttgcgtctag caacgaacaa tagaccgctc ttaatcgcaa
    25141 tgccagctac tgagcggaga gattcgattt tacctgctgt atcatcttgt ccaaccatct
    25201 gtatttctca ctgctatcta tcattatcca tatcggtttc catggctccg ctacttatgc
    25261 gagcacaggc attggctagg tctaaaggag taaaccatcc ttttttgagc caaagctgtt
    25321 ttccagctaa gccgtgcacc aatatcgccc ccaataatgc tctcgccaca ggattatcag
    25381 aagaggtggc aggttctgtc gattctcggt tttcttttct ttcctttgag aaagctatcc
    25441 atcgagcaag aaacccccct atcattccag acaatacatc tcctgatcca gcaattccta
    25501 agcccggagt actgccatca tatatgaaac aggtaccatc cggaagaccg acatgagtcg
    25561 tagaagccct tagggcaagc gctatttcat atttttttgc aagagcgctt accgatgcca
    25621 cggtctccga agtcttacca gaaaaatcgg aacccagcat tgggatgaac tcgctaaatt
    25681 cgccgggatg cggagtcagt ataatgggcc ctccaaaaaa gctagaatcc tggtttgctc
    25741 ttgccattct tgcaaacagc cgagcgccat ctgcatcgag cacaagcggt atatgtgctt
    25801 gcaataacat atccagaatt ctctctcttc ccggatcgat tccccatcca ggacctgcta
    25861 tcacaacatc ccagcattcc gatgaaaatg aatcctccga aaatacgcga acaattgcac
    25921 tctctcctat agccgctaga taagctgcga atagttcttc atcgcagaat agggcgatat
    25981 atcctgctcc agcagatgct gcaccacgta cacagtgcag agctgcccct gccccgctgg
    26041 cgcttcctgc aaacacggct actcgccctc gctgcatctt atatgcccat ggagagatgg
    26101 ggggcaagaa gcccttcata tctccttctt ccaaaagcca tgttttctgc gttgatgcct
    26161 tcgtccataa ggggaaaata cttcctactg gaataatgtc tccagcgtat ggtcttgctt
    26221 tcggcatata taacgattct ttcattggct ctatgcagag cgttatatcg gcgcagacaa
    26281 tcggaaactc cggtctccat ccatcgctaa gcccggaagg aacatctacc gatacgatga
    26341 gagggttctt tttgcacctt tcattgcgag caattcttac atctcgcaat atttggatca
    26401 aatctgaagc tctccccctg gcgggtcctt ctattcctgt tccaaacaag gcgtcgagta
    26461 taatatcctc tgtggaaagc cgttcaccga tgctttttaa gtcgattgtc tcataacaga
    26521 tgatctccgt tccacatcgc tctgctcgac gcaagtttga gatgacttca tttttcatgg
    26581 tattgggttc gggtatatat gcagtaagcc gagaataacc ttccaatcta gcatgccgca
    26641 acattgccag ggcgtctccg gcattgtcgc cttttccaca tactgcagct atgccaggct
    26701 gacgagaaga tgatgatcca gcggagatca ggcttgacat attcttcttg acaattactt
    26761 cagataaaag ggtctgccac aagcgaattg cggcaatttc cattagatgg tctacagaaa
    26821 aattgaattc gcttctggta agcgcgtcca gctcaataga accgctattc gatatcagga
    26881 catgcatgtc aaaaggcctc caatccacta tactctcaca aatctcgtag ggcaagcgaa
    26941 cacccgatga ggattattgg gctcgaagat atgctgatta gctacctttg cataaaattg
    27001 tcgtgacccc cgaaggtacc ccccaaagag atgcagtgaa gtcttgcaag agtcagcact
    27061 tttcgtatag cgttatagca tgaataattt tatgagctta gggataaaca cgagtctcat
    27121 acaggctcta gaaatacttg gcattaatga gccgacggaa gtccaaaagc aggcagtgcc
    27181 cctcctcctc gaaaagaagg acctaagcat gcaatcggag acaggaacag gcaagactct
    27241 cgcatatctt ttgcctatct acaataccat attgtcaaat atcgacgaag ctgatagaag
    27301 atggcccgta gcagtcataa tctgtcctac ccaggagctg gcggtacaag tagccaatca
    27361 ttcggctcag ctagccatgg ctctgcagtc aaaaaataac ggtgctatgt cgcaatctga
    27421 ttcatgccaa cagcaaggat taagtaaatt acgcaatcga gctcccaaga tcgattcgaa
    27481 tcttttgaat tctccacagg ttcggagtct tgtcctcttg ggagggactc aatttacgcg
    27541 tcaaaaacaa gcgctaaaaa tacaccctca tataatagtg ggcacacctg ggcgccttgc
    27601 agacctaacg cgcttgcgct atttcgatct ttcacatctc caattcctta ttcttgatga
    27661 agcagatagg ctattctcca gagaatacat agaaccagtg gaatttcttc tctccaatgc
    27721 ccccaaaaac tgcacgcacg tgatggcctc tgctaccatc ccggagcgaa ctcgtaaccg
    27781 agcaaaacca tggataccca agcccatcgt aatcgaactt gaaagggaag gagtactatc
    27841 tcaagccatc gagcattggg cattctattc cgatcacaga aagaagatag atcttcttaa
    27901 gagaataata gtggcgatac atccgaaacg atgtttgata tttgcatcag aagcttatcg
    27961 cgtccaaagg gctgcggaac gcttgaactc ttccggacta caatgtgcca gccttgtatc
    28021 tagaatggaa aagcaatcaa ggcattccat aatagaacaa tttaagacag gcggtatttc
    28081 tctgctcatc acaacggatc ttgccgctag aggtctcgat atacctgata taacacatgt
    28141 gataagcctg gatttgccag aagacggcgg agtatatgta catagagcgg gccgtacggc
    28201 aagagcgggg aaaaagggca taagcattct gatcgcggat aagctcgaac tcgaacgcgc
    28261 ctcaaggacc gcaacacgat ttggctttgt atttcgtaca aaatggcttg aatacggaac
    28321 cgtaatggaa ccacctgtcg aggcattttt cgagcgagtt gaagatatgg aaacacaaaa
    28381 gcgctcgaag agccatctat gagtgcatct acaatcagtg aacccaaaat tgtcacagaa
    28441 gcagtggctg cagatatagc caatccgcga ttcgaggatg ctcagtatag gattctgata
    28501 gttaggctct ctccctttcg cgatgtcgaa ggttcgtata cgcaccaggt actctttgat
    28561 gaagcaaggc atgctcttcc caaagcctat atcgactttg catttatgcc gagcttttct
    28621 catcgaaaag ccctttcaat tgcaaaaaga ccttggtttt ttgggcgtgc atccaatagg
    28681 agtgccacag aattcaatat gcttctaata tcttgtgcat tttctctgga actattaaac
    28741 atcccttggc tactctctca atccggaatc cccttttcaa gacatgaaag gttagaaaga
    28801 tatgatctgc cttttctctt cttaggagga tcgagcgcag tctgttcagg ttcgcttgtg
    28861 aaaatctccg acaacaaagt tatcaatagt cttgtcgacg ccatattttt tggtgaggga
    28921 gaaggcagaa tatcggagat tgtgcgcatc gcatctgatg attcaaagac gggcttatca
    28981 aaactgtcga tcctagccca catagcctct gaagttgagg gattctggcc ttgtgattca
    29041 agctttacct gcaatcgagc actttctttt cagcgcccgg cagttcttac atcgcctatt
    29101 gtgctgaata gcgaaaatgc cgataccgtc aagctggcaa ttagcgctgg atgcataggc
    29161 cactgcacat tttgcctcga aggttgggat agaagacctt atagtgaaaa gccctccgac
    29221 tatttgtcca aatcggcagt cgtactcaaa agagcaagcg gcgcctccga tgttgagctc
    29281 ttcagctaca atttcaacat gcataaggac ataatacagc taatccagat cttaggccgc
    29341 tattttatga aagtctccat gatgagccag cgcatcgata tactatccaa taaacctgaa
    29401 attcttgccg cggagctggc tgcgggaaag cgttcttttg cccttggcat cgaaggaatt
    29461 tctgagagaa ttcgtaaata ctaccaaaaa ggtatatcag aagggcagat ttggaattcc
    29521 ataacaagaa tcatcgataa taaagcacga gaaatcaagc tcttttttat tatatcagga
    29581 ttcgaaaatg gacatgactt agaagaattc gaaaatttct gcgataaact agcccactat
    29641 aaaatagaaa ctcagtcggt tactagagta atcgtgtctg ccggttatct tgtcaggttg
    29701 ccctttacac ctctgcaata tgctccattg caaggagata gggctctact gcagagcatc
    29761 gcatctgccc ttcgcattag ctgcaataaa tcaaatcttg aattccgact agcatcctct
    29821 ttcgaggatt attggatgga tcagctgctt tcgttgggcg gttcaagaat gcatgattgg
    29881 ctccaagctt gccctgagaa tgggttttta tatgatcttc atgtttctga tcgcgcattg
    29941 aatagccttt gtgctttctt caatagagag tcttgcttta cccaactatt gcaggaaaag
    30001 catcatacct atcgtcctga ttttgatttt ttagaaagcg ctagacactg gcaaatgctc
    30061 gcagagcttt atgatcgatc catcgatcat ctatgttcta cggattctaa aaagttctat
    30121 acaaaaatgc cagcaattcc tctaatttcg ctagaagcag aaagaactat cgacattatc
    30181 aaggcccaac aaaaagcgaa ggctcatttt ccttcaattc ttgttagaat aagggagaac
    30241 gatgcactgg ctttttcgac tccagcttat gaacgcgctt ggcttcttag aactttatct
    30301 tttttggtcc caaatgcgga acgagctctt ttttattgca aattgcagct cccaaactcc
    30361 gattgggaaa gcgctattcc gacaaaatcc gcctctatgc cctgtaaatt gaggattggc
    30421 atatcaggag tgaaatactt cgccatctat ggtccggata aaacggacat ggagagaatc
    30481 atcaatcttg ctatatcgaa gataaagcat tccaacgaga ccaaaaagat cggactatct
    30541 tcaatttcaa gcaactattc tggatcaaca gccttcctgg aagaaataga gataattcaa
    30601 gagcttcctc aagcggaaac ctgcaggctt tccttttgta ttccagcaaa tagagacgga
    30661 tttcttaatg aggtccttga gggatggctc aatgagttga atctacattt tacattgcaa
    30721 aaagatgaag atagtattat ttatcagact tattcagctc ataaagctaa caaatctgtt
    30781 caatatatac aatataagac atcctatact aatatatctc tatgcctatc tattggtaag
    30841 aaggcggata tagcattgct ggcggatttt ctctatgata aaaaatgcac cctcgaagag
    30901 acaatatttc gaatcgaggg gtgggatcta tcgaatgtat gtctggcatg atttatcgag
    30961 catcaggcca tgcgtcgggc acaaaacgcg ccatagaact gcttgtctcg aaaacatcga
    31021 ctgccgagat gggcaaggaa gagaaatctc tgtgtaatcg gctatagatc agagtggcaa
    31081 ttctctcagc gcttggatct tccgctaatt cggctatttg attcaaatct tgatgatcaa
    31141 attcctcatc gatcaatttc tttaatgccg attttaatat gccaaaatca attagcattc
    31201 caccctctcc gagctctttg cccgaagccc agactcttac tctatagtta tgtccatgaa
    31261 aacgttcaca ctttccgttg tagtgaaaaa gtctatgagc tgcagaaaac tcagtttcca
    31321 ctctaatatg atacatttta cccttccttt aatgggctag aaatcattga gcttaaaaaa
    31381 ccctattcat gatggccgaa acggggcttt ccaatctcca tattatatat gatttttcga
    31441 aaaacatctt gtccgtgcgc tcataatcaa tgcttgcctt aagattgtta agattcattg
    31501 cagctctctc ctatcttatg atagaattcg acacaatatg cagaaacggc tgtctgacct
    31561 gatacaagga ctcgatatct gctcaatcga aggagatatt catcatttga tatcaggcct
    31621 tttctatgat tcgagagaat gcaagcctgg atccctgttt tttgcgcttc ccggcctcca
    31681 tacggacggc aaaaaataca ttatccaagc catacaaaat ggtgcagttg ccgtgatcta
    31741 tgaaggcctt ctagagaaga acttgaggga atttatgttg aaagatccat cggcaagagc
    31801 tctaaatcct gcattgatcc gggtcacaga ttgccgatgg acgatgtcgg ctatatccgc
    31861 aagttttttc gagaatccat ctgattctct atgcgttatc ggtgtaactg gcacagaagg
    31921 caagagcaca acagtatcgt ttatctacca acttctgaat gctgctggtt tcaaagctgg
    31981 tttcttttcc actgtaatgt cagatattgg agaaggggaa cagccaaatc ctcagcacca
    32041 gacaactcca gaagcaacca cagtacagca gatgttggca gaaatccgca accatggatt
    32101 acagtttgca gttatagagg catcaagcca tggcttatct ccacgaaccg caaggttagc
    32161 ccatgtccat ttcgacattg gccttgtgac caatgtaact catgagcatt tagaattcca
    32221 tggctcatgg gagcagtatc gcagcgacaa ggcgaatctt ttccggcgcc ttggcgaggt
    32281 cgaacccaag aaactcaata aagttagtca tgcgataaat gaacataata gcaatataat
    32341 tatctctcct actggaatta tctgcgccga tgacccaaat gcatcatact ttatgcatga
    32401 gtctgcggtt ccatgcctga cctattcgag caagggagcc ctagcagatc tctcggcaca
    32461 caatataata gaagatgcca atggagtttc ctttgagata gaggggcttc tggaaatgcc
    32521 tatcgggcat aacttccgca cagaacaaga aatatctgct cgaagattcg aaaatgttat
    32581 gccttcgaca ttatcggccc ggatcaatct tccaggcacc ttcaatgttc aaaatgcact
    32641 tggagcaatt ctagctgcct ccgccgcaac gggcttgcat tggcacaact tcgttcctct
    32701 gcttccaggc cttaggccga taaaaggcag aatgcaacga attctagaag gacagccttt
    32761 cgaagtgata attgactatg cccatacgcc atcgtcattt aaggaaatac tcccctctct
    32821 gagaaaagaa agaagaggtc gcatcatctg tgtctttgga tcagctggcg aacgcgacac
    32881 cgaaaagcga ccacagcaag ggcgcattgc tgcggattat tgcgatatat tgattcttgc
    32941 cgatgaagat cctcgaggtg aagaccctat ggccatcctt gaagaaatag ccatgggttg
    33001 tcctgaactc tcaagagaag accgactata tctcatacca gatcgcccag aagcaattcg
    33061 tagagcattt aatcttgcaa agcccggaga tcttgtcctg cttctaggca aaggtcatga
    33121 gaattcaata atctattcag acaggactat cccctatgat gaagaaaggc aagctcgatc
    33181 gatcctggcc gacttgggat ttgaaatcac aaataaaggg aaataatcta tgaagagtat
    33241 cgctgtactg tatggtggta gatccggcga gcacgaagta tcgcttatat ccgcgagcag
    33301 cattttggcc tacctcgata aggataagta ttcgattgta ccaataggta taaccaaaag
    33361 aggagaatgg ttcctccagc atcctccaga atgggcttat tccaggcccg aaaatgggca
    33421 aatgcgctct cttcctcccg tcgaaagatc tgaacgcgtc ttagcagtgc ctggggaagg
    33481 actttggctc gagacaacaa caaaaggcct gcaaaaactc aatattgatg ttgtatttcc
    33541 cgtgctgcat gggacttttg gagaagacgg tacgatccaa ggtctgctcg aaacggtaga
    33601 tcttccttat gtgggagcgg atgttttggg aagcgcggtt ggcatggaca aggaagtttc
    33661 taaacgcctc tggctgtcgg ctggtctacc ggtggtcgat tatatttcgg ttagaaaaga
    33721 agatgtttcg gatgaaaacc ttaaaatact tgcacgcaaa gtagaaacaa gatttggctg
    33781 gccttgtttt gtaaagcccg catgttcggg ttcctcggtg ggaacttcta aagtcactca
    33841 ttcagaagag ctaaaagatg caatactgtc tgcccttcgc tggtctgagc atgcattgat
    33901 cgaggcattt atcaatgctc gagaaataga atgcgcggtg ctaggcaacg agcgacctat
    33961 ggtctttcct cccggagaaa tcgttccatc tcacgaattc tacgactatg aagcgaagta
    34021 taaggatcca cagggcgcta cccttttgat acctgctccg ttgttagaag agacaaagaa
    34081 tcgaataatg agccttgcgc tttctgcata taaatatgcc tcggtaaaag gaatggcccg
    34141 agttgacttc tttgtagaga agataacagg agaagtattt ttaaatgaga taaacactat
    34201 tcctggtttt accgccattt ctatgtatcc tcgaatgtgc atagatgcag gagtctctta
    34261 ttctgaattg ctggataaac tccttgaact tgcgatagaa caatatgcga taaagcgtga
    34321 gctcgaatat gatttctcaa aagcataaaa ttttctaata atcatctcct tagactcaga
    34381 atcaggaatt agccatgagt cagctttacc caaaaattca gttttagtta atgcataatt
    34441 atttatacta tttctaatta ttattatcac ttccgcattc aattgcctcg ggttataatt
    34501 agcattgaaa gacagaccac tccaataata tcaaaaggag gaaatcatga attcacagga
    34561 ttatatagct ctggattacc aatacggagc acataattat catcctatcc ccgttgtcat
    34621 ttcaatggcc aaaggatgca aggtctggga tcccgaaggg cgagaatact atgattttct
    34681 ctccgcatat tcggcactaa accaaggcca tctgcatcca cacattgtca acgctgtcaa
    34741 aagtcaaatc gaacgagtga ccctaacttc aagagccttc cacaacgata aaatgggacc
    34801 ctttctcaag aaactatgtg agtatacggg ctatgaaatg gctcttccaa tgaactccgg
    34861 agtagaagct gtagagacgg caatcaaggc tgcaaggcga tggggtgtag aaaaaagagg
    34921 gatagaaaat ggaagacagt ccataatatg cgccaatggc aacttccatg gtagaacgct
    34981 aagcgctgtt tcgatgtcaa gtgatcctga ttcctatata aactatggac cttatgtgcc
    35041 tggctttata aagattcctt ttggagatgc caaggcggtc tccgatgcca tagatgagaa
    35101 tacagttgct gtgttggtag aacccattca aggggaagcc ggtgttgtcg ttcctcccga
    35161 tggctatctc tcggatctca gaagaatctg caccgaagcg gggatcttat tcatactcga
    35221 tgaaatccaa actggttttt gcagaacggg ccgccatttc gcctggaatt acgaaaatgc
    35281 ccgtcccgat attatgtgtc tagggaaagc tctgggaggc ggtatcattc cgatttccgc
    35341 tattgttgcg gatcatgaag ttatggatgt attcacacct gggacgcatg gctcaacttt
    35401 tggcgggaat ccgctcgctt gcgcggtggg cattgctgct ctagaagttc tggaacaaga
    35461 aaatctcgat gaaagagcat ttaggctcgg agaacgcttt agggaagtca taaaaaaagc
    35521 aaaatcaccc cattttcgct tggttcgcgg aaagggtctc ctaaatgcag ttgtcttcga
    35581 ggagggcttt gatgcctatc ctacttgcat agacttaaag gaatctggca tacttgctaa
    35641 acagacgcac ggaaatataa tacgctttgc cccaccgctc gtcataacag aggacgagct
    35701 cgaagaagct cttgagaaaa taatctacgt gctggacaga gcctagaatc taatttgcag
    35761 aggatgatga ataatttcaa atccttggtc agccatttgt attttgtggc tttttttgcg
    35821 gcctttttga ttgcagtata cctgattgtc atacaaaggc aagagacatt acaggcttcg
    35881 gtgccaatag agaatcgaac tattgtcctc gaaagcctat cggataaaag ccaggtttcg
    35941 cctcaaaata attcactaca gcatgcaaag atatcacctc ttccgggtga tatctttctt
    36001 aagaccatag acatagatgt cgacgatgat gaggattttg agcaagttat tctatgcaag
    36061 aaaacggctg gaagcacggt cttgtggatg ataatagctg acttttcgcc aaatcttggt
    36121 gcttacctta ggcattttga agcggagata cgggcttcaa agctagagag cataaccgtg
    36181 cagccacttg atgttacagg tgatgggctt tctgatcttc ttatccaggg cttggatagc
    36241 tctaacaatc aaaccctgac catattccgt cggctattaa atcgagggta ttctctcata
    36301 ttttcggaga caggcctatc cataaatctt caggactctg atgagtctgc tataccatct
    36361 atcgttgtcg aatcattgcc aaataaaaca ggtctagttt cctataagac ctatatatgg
    36421 aatagaaaca gcctgttctt ggagaaagta tcggaatctc ccttaagaga gagcaatagc
    36481 atctcagaag gtccttctgg aagcgatgct cagtcatatc tatcctggct aaatcggttt
    36541 tggaaaaaga actcagatgc ctccaatata tgctcgctgt atctcgatac gaatagcaat
    36601 gcactgatct tggaaggcca acagattcag cagcgatgga taatcaaaag tgccgaacgc
    36661 aaaggcaatc gactctatct tacctgtagg ctctcagaac tgagttatct cgaccgaatc
    36721 tttataatag acgcaaaagg acaaaacgtg atatcggtca gcataatcga tcaacaagta
    36781 tctcatttcc gtcgcgatga gggttggtct gggaaatatg aatcctatga ttatatgaag
    36841 gaagctaaag acaataagac gattctttca agtcccgatc attcgcaatt cctgggccgc
    36901 tatcttggtg atgaccattc tgtgctagtt ctcgatcagt ctaagaacat ccttgtatta
    36961 aatggcaaat atagagaagg ggcagcaaga atctactatt atgatggata ccaggtgctt
    37021 gatttcttgc agattcagtc gaatggactt gttggagata ggttgctctt cattgtctcc
    37081 atcgatcaat ccgagaatgg caaaataaat cgcctgatcc tcagaccggc tgagataggg
    37141 gcggatggcg tcagaatgga atatcgaaca ccctacatct tcatcaaaac aagttaattg
    37201 cctgcaggat aagattcata tccaaggtct ttgagcttgg attgagtctt cttccaatct
    37261 tcttcagctg ccacatggac agcccatctt ggttcgccgg cttggttttt caattctatg
    37321 actcttgtct tgaacttctg attagtcaga tttctggaga gacgttcggc gttatctttc
    37381 gatgaaaaat agcctgcttg tagccattgg attccgcttg cggcgctatg ttcttccgct
    37441 atttgagaat tgttcgatgt tactccataa tcagcaagag gcgctgggcc cttaatccac
    37501 gaaaagccta aaaaaagcat aagcgatgaa gaaggaaaaa ctcttcctat aattaaatcc
    37561 gattctattg agttagggaa atccttgacc aagctagaaa taccagcccc aaactcacca
    37621 aaatccgcaa aatattgcag aagaagtagc tcccttcgca attgaggctt tgtaacaata
    37681 gccagggcat ctctcgcttt tgaagaagcc gacgcatatt ctccatcata gagagaaagc
    37741 caaatatcaa aaatatgctt gaattcaggc gaaatggact catttttcaa gagcgccaat
    37801 gcatttttct gatcgccgat tgctattgaa agtcttagcg cctcattagt gtattttgcg
    37861 tcgatagaag cggcttttat ataccacgaa gcagcttctt tcgtttgtcc aagaagcaga
    37921 tataacccac ccagaatgcc cctgagctcg gcttttaagt tttggtcggt ttttgcctca
    37981 tactcttgac aaagcgcaat tccatctctg acagatagct gcgaaactgc cgaaagcaag
    38041 acatccctca aagcatcttg gctcttagtc ttggacgcag cctgccgtgc ttgactcagg
    38101 cttttgtaag tttccgcttc tatggatgag ttaatgataa gaatgaataa tgataggaaa
    38161 agtacaaatg aggcggcgaa atttagcgaa gagcgatccc ttgttatccc tatcctattt
    38221 cttttcacac ttttcctctt tacacttctt cgcctcttaa tgtattgcca acaaccgata
    38281 tgaaccttac ctgggcaaat tgcccaattc ttgtcgctga accaggaaag accaccatct
    38341 catcgcgtga agtcctcgct aagagctctc ttgttgatcg ttttgatcga tcttctatga
    38401 gcacttcgtc gacatggcca atccgctctc tcataagaga agaggttatc tctttttgaa
    38461 gagcaatgac ccgggcgagc ctttctttct ttatcttttc agttatctta tttggcattc
    38521 ctgcagcaaa tgtgccattg cgttcgttga agtgatacat aaaagcatag gagaaacgaa
    38581 cgctgcgcat aaggtctaat gtctgttcga aatcctcttc ggtctcgccg ggaaagccga
    38641 ccattatatc tgtagacaat gtaaggtctg gaattgcatt tcttagagca tcgatgacag
    38701 aaaggtagta ttctcgagtg tatttgcgat tcattgctga gagtatggta ttagagcctg
    38761 attgcagagg caagtggaga tgatgacaga aaaaaggatc ctctgccata actgccatga
    38821 gctctttaga cagatctttg ggatgacttg taataaagcg tatccagcca attccgcgct
    38881 tagatttttt aggtgacgca ttgtagcatt ctatcgttcc ttcgctctct tgcttttttc
    38941 gcaggtgcgc tgcaatcatt tttatgagcc caggaaagtc caaagatgcg ccatcttttt
    39001 cccaggtata ggaatttaca ttctgtccaa gaagaattat ctcacggaca ccaagatctt
    39061 ctagtctatc tatttctcga agtatgtcca aaggactgcg cgagatttcg cggcctcgca
    39121 cataaggcac tatgcagtag gtgcaaaagt tgttgcagcc atgaatgatg ggaataaagg
    39181 atcgaaatgc gccagtctcg tgatggtcct cactaaaaat atatatgggg ttctcctcaa
    39241 gcacttcgat tttttggcca ttggcaatgg aatcgagcat tagcgaaaat gattgccttt
    39301 ggtatgtccc aataatatag tcaacgccgg gagccttttt tttaatggcg tttttatact
    39361 gctccgccat acagccaata accactagaa cgaaatcatg ttctttcttg cgcgcagtca
    39421 gttgatgtat acgattccat actcggtttt cagcggttgc tcttacagta caggtattca
    39481 ataagataag ctgagcttct ctctcttcag attgcttcca tccatgttct ctaagggtcg
    39541 cttccatggc tgccgattca gccttgttca tttcgcagcc ataggtctcg atatgataat
    39601 tattcatttc ttctttttaa ggcctctaat aaatccagca agattgaaaa ggccatatcc
    39661 aagaagtccg atgccaccca gggaaagcag cgaatgtgct acgcctggca gaaaaagagt
    39721 ggtaagtcct gcaatgccag ctacccctag cactgtaccg gaaatcttgt cggtctttgt
    39781 ctttcctaaa agacctatag cgccaagccc tatgagtcct gctgatatca cagtaccgac
    39841 tacggggata ccaagcagag cttcgacacc caaaaagcct aggcctgcaa ccgaagacat
    39901 aacacccttt gtccctttat cattcaaagt cttgggatca accggataat tgctttccat
    39961 atcttttcct ctcttaatcc aaaatgcatc atactccaga attattaact ctaatcgaca
    40021 ttcctcacat caaggataat aagattcaag tcgatccagc cttccatgtt ctccactgac
    40081 aggttcaatc ttcgagcgat cgcttttttc gacatatgca taagcggaat ggttgtggat
    40141 actctcgaaa ttttccgctt caaccgaaaa ccatggaaat cgttgaagag catcaacttt
    40201 taaatatact tctctcacga catcttcaac aaagcgaggc tgcatatagg ccttctcagt
    40261 gatatacttt tcatcttcgc gcttcagcag agtaaacaac tcgctcgatg ccgattcctc
    40321 tattattcgt atcaggtctt caaaccaaaa aaatgggcca agagatactt gaagtgtcac
    40381 aatgcctcgc tggttgtgtg ctcctccatc ggaaattgct ttggaacatg gacagactgt
    40441 ttgcacgggc actattacgc cggcaataaa acgatgtcct ttccccgaac aacttgcttc
    40501 ataaaagcat tcatagttca taatagatgc ttgttccgag acaggtgcct tcttcttgat
    40561 aaaataggga aaatggatat ctgtataggt tgtctgagca tccagttctg ctcttaactt
    40621 tctcagcatt tttatcacat tgggcatcga gaggtcggtg cggtattcct caaagacctc
    40681 tatgaatctg ctcatgtgag tacccttgaa atcatgtgga aggtctgcaa ataaattgac
    40741 cgcagccgtt gtatgctggt atttttcggc cttatcaagc agagttatgg gatatcggag
    40801 gcctttgacc ccaaccttct ggattgcgac atttcgatca tccctaaacg actgaacatc
    40861 taccatgatt ctaacccata ttccctatca gagcacgtac aaatatctta aactcatatt
    40921 ctctctgtct catgcaaact tcgattttca aatcgcgata ttggaaatcc tcgccgcggc
    40981 gtcgatagtg tttgagagta acatcgtgat agtcataggc cccacccctc ctggcacggg
    41041 agtaatccag cctgcaatcc tcgaagctcc ttcaaaatcg acatcaccac agagtcgata
    41101 acccttcttt ttagtagaat ctggcacacg attgactccg acatctataa tacaagcgcc
    41161 tggcttgatc atatccacag acaccaagcc gggtgctcca gcgcaagcca ccacgatatc
    41221 cgcttcgcga acatgagagc caagattttt cgtccctgtg tgacagatag taaccgtcgc
    41281 gttgacagat ttccggagaa ggaggtttgc aagtggctta cccacaatat tcgagcgccc
    41341 gacaaccact gctcttgccc catttgttgg cacgccactc ctcttaagga gttctattat
    41401 tccaaatggt gtacaaggta tgaaagaggg ttgttccagg agcatgcggc caagattgac
    41461 tggagtaaag ccgtctacat ccttattggg gtcgatggcc tctataacac ggcgttcatc
    41521 gatatgccta ggaagaggaa gctgaaccag gatgccatct atattgggat ctctattgca
    41581 ttccataatt ctatcgagga gagcttgctc cgatgaattc tccgccattc ttgtctcaaa
    41641 gctcttcata ccgatctctg cgcaggcttt ttctttagcg gtaacataag agatcgatgc
    41701 tggattatcc cccacaagaa tcacagcaag acctggcacg actccttttc ccttaaggaa
    41761 gttgactttt tggacaagct catttctaat ctcctcagca attttctttc catcgataag
    41821 tactgcagac ataagtcctc ccatcttcaa gatactatat ttctaagatt tcgtcatgaa
    41881 ttctctaacg cttcaaagaa atgcctatat tctgtgcacc agcgaaatgc gctgatgctt
    41941 ggaatgatca ggctaaaggc aaaatgtact ccattatcaa tatcgaaaaa aaatcctggt
    42001 tgcgcaaaaa tccctttctc ctccagtagg cgctgggcta ttctatcttc ttcaaaaaag
    42061 ctagggcttt cgattagtgc ggtccatccg ccttgacagc aaagcactct atgagggctg
    42121 gtgccacctg ttagaatttc tcgataactt gcataattct cgcgaattct gcttagcgac
    42181 ttacataaaa aagccgtttc atgttcaaat agaaaaggca gggcattcat tataggagtg
    42241 ctcacagaga gaaaagtatc tgcgatgaga tcgagcctct tttttgctct ggatacctct
    42301 tggtttggcc cagatagggt gatccacgca agcttcagct gaggcagacc cattcgtttg
    42361 gagagtccgt cgagtacaaa ggtgagcacc ctatcctccc ctacgaaggt ctttcgttcg
    42421 atattatcgt ctatgggaaa atcgaaaaag acttcatctg caatcaatgc gagaccatat
    42481 ttttcgcata gtcctataat tgtggttctt tcagtctttc gaatgtatga accggtagga
    42541 ttattgggat ttattataac aatggccttt atccgctttt cacggtccgc tgctaaaaca
    42601 gaagaaagct catctaggtt aacataccaa ccagaaggat gagcatactc aagcctatag
    42661 gccagcactt gtaccgaatc cagttctgtc aggtgttgaa agagagggta accaggcttg
    42721 ggtacgagta ccgcatcgcc cgggtcgcac agcagcttga aaagccatga atatgcctcc
    42781 gaagtggaag cacatagaaa atagttatcg ggatgcatat tccgattgta taaagcaaaa
    42841 tgatccgaga tcgcctctcg ggcgcgataa agacctctag gttcaggatc ataggtaaca
    42901 ttatctttga ttgccaaaga aataagatcg tgatctttcc aatcgagccc tacctttgtg
    42961 ggatttgtat tagataggtc ttttatcgga agtccaaaac catgcctccg ggcgcgaacc
    43021 ctcgagagct cattgggttc tatttctcca gcattggatc gtcttgaaaa aatgtagctc
    43081 atcgaaaaaa acctatcgaa gtccaagaat actcggaatg ctcttctcaa gatcctgccg
    43141 aagctgagtt gcttcaattt ccaaagaatc gcattctttg cttgccgtac aggcaaattg
    43201 ttcatcagac aacgtcttta tgttgattcg aacattcaaa acagctccag aaagccctgc
    43261 ccatgccatg agacatcctg tggctgcatc ggtagctgaa ttcaagttac cagtctcgac
    43321 tgcttccagg cataaacgca tagcctcaag acaaaaccga gcagtcttaa gaggcacgcc
    43381 agccgccagt ttgttagctt tctcaattgc caaatctcga gctatcctct cggcatcggt
    43441 tgatcttgga agcctcatag catccatcac cgcattgaaa gctgccgtat cttcatcaac
    43501 gatttcgaga agatcattct ttagtctctg agccttgata cccagtgtcg ataatgtctc
    43561 cgaaacacct tcgtaacctt ttttgcctac agtaagattt gcatccatcg cagccagagc
    43621 agaaccaaga gctcctgcca aggcagccac agaccctcct cctggtgctg gcgagtccga
    43681 agagactgta tccacaaaag aactgacctt ctttgagaca agagcccctt cttttcttat
    43741 tgcccactca atgatctttt tttctggttc aaaaggctgc acagagcgca agccaagtgt
    43801 ttgcactgcc aactcgacaa gttcttcatc cgaaagtcct gggcttttgc ctgctttttg
    43861 gcgatagtat cgcccacaga cgcatatagc ctcaagagga ataagtccaa taagctcaga
    43921 accgattaca tatattcctc tggcttccgc ttcctttttt accgcatcga ataccacata
    43981 caatggggtt gtgcgaaaat cgagaatatt gatagatacc tgggcgcatt ggtaggattc
    44041 gatataccat ccaatagcgc ggacagcctg caataagcct ggaaccttga tggtattccc
    44101 attgctgtct cttattgtgc ggcccgattc tctaatactg agtgctatat cgtgagctac
    44161 tctcttatct ttggtgttga gatttatatt ataagcaatc aagaattctc ttgcgccagt
    44221 taccgttgcg ccccagcgag gggtgaaatg tgtgggtcca aagtcaggct gccatgccgg
    44281 atccgcaagt ttgttctcta atccttcata ttcaccggca cggatatcgg caagactgcg
    44341 gcgctcggga cgagtcgctg acttttcata aagatagact ggcacattga gctcttcaga
    44401 gagcttctta ccgaagtccc ttgaaatctc tatgcattct tccatggaaa cacctgatac
    44461 aggtacaaaa gggcacacat ccagagctcc tattcgtgga tgagctccac ggtgcgtcct
    44521 catgtctatc agcttaaatg caacccgcgc tgcttccagg gcagcctctt ttacagcttc
    44581 aggaggccca atgaaagtat atactgtacg atttgtgtcc acacccgggt ctacatcgag
    44641 cagaaagact ccttgaacag atctgatggc atctgctata gcctcgattt tggcgcgatc
    44701 tcttccttcg gaaaaatttg gaacacattc tactaagggc ttctccattt tattcctcca
    44761 ttatatggaa ttcatccaga cccaatactt ggcgattagc caccttccga gcccgttcta
    44821 atgatttacg atcattgata ttttgaaata cgattccagg atcgcagata gaagctatat
    44881 cattctcctg aataatgaga tgtttttcca ttttgataaa gtgtctaaag gataagtcag
    44941 aactgctttg aatataagaa tagagttcag agacaagatt tctcttatag aatgcaaaaa
    45001 aaggctgaag ggtatcatct gtcttgcaga gaactatatc cacttccttc gacatggatt
    45061 gaacatagag atatcgaatt aatgccaaag atacaaatgg catatctgcc gcacaaaggc
    45121 aaacccattc caaagaagca ttgactagag cagagaatag gccacatact ggaccgcctt
    45181 cctttatatc atcctcaaat tgagaggcac caaggtcttc atataacttg tgaggcttgc
    45241 ccacaatcag cagctcttgt gcaatcttgc ctaatctata ggcgagcaaa aaaccgattg
    45301 gaactccttc tatttcgacc tcttgcttgt caattccacc aaaacgcctg gcatgaccac
    45361 cattgagaat aactgctgag aacggcaacc tttgctccgc aatcaagccc aagacttctt
    45421 gttctgcttt tgtatctgcc ataggatact attatgccat gttttaccaa attcatgcag
    45481 atactcaaat gggcagattg gtccgctcca ccagaacaga tgaactgctc cctctctttt
    45541 caatgtataa ttcagactat taaggagctc atccctgcca ttgaatccgc tcagctgtcc
    45601 gacgagaaac tcgattctta tagaattcaa gagctgcttg ataaggcaat ccgcttgtat
    45661 ctgctgactc cagctcttgt aaacgtactg ctaaactata aaatctgtgt ggaacatgac
    45721 ctgccgcttc atcctaccgt atattacgaa ctcaaagagg cgcgcaagta ccgaatacgc
    45781 cattcactag ccgaaatcca gcaagccaat gaacaatact ggcaatctat cgaggttgca
    45841 cgcttgtgtt atcaatgtgc tcctcaagcc atatcagcca tagatgagct ttgttttgga
    45901 attccatcag gcattgcttc ctttttgtat acggcagtgc aagatcatta cacatggcta
    45961 ggttctcagc cttcactcct attggaactt gcggagaaaa tttccagaga atttcgaccc
    46021 agtctcattg tcgccgcagc tcatggttcc atcatgcccg ctcttatact ctccgaactt
    46081 cttgaaatcc cggtatattt tattcgcttt tcgatgttta aacgccacga tgaagaacct
    46141 ataataagcc tgtcggatca agcatggctt tttgactatc gcaataagaa tgttcttcta
    46201 tatgatgaag atgttgccag agggaataca ctggatttat tctcaaggag gttatctccg
    46261 ctgtttggag aagtgagaac tgcttgttcg attcggcatg ccggttcatg cgtccatgct
    46321 gatttttcgg gcagggtttg gtgggactga gtgtgccaat gatcctaagc gattcctttt
    46381 gttaatttgc ataaattgca aaaaaaatcg caataagttg catatccatt agccttatga
    46441 tatacttatg gatagaaaca cttgaagaaa aatgatgcga tatgcctttc atgagcgttg
    46501 gcgctgaggt ttacctcctt ttcctcagcg cctttctttt ataatatggt tgaacttttt
    46561 ggataccgga taatgttgat tcaatcgctt gacttttatc gatacataag tataggttaa
    46621 cataagtcta ataattagtc tctcatgcaa agctattaga ggaaccctga tgtctcaaaa
    46681 aacgaagctc aactatgtcc cttcgatccg ccgttttcct tcatatctcc atataatacg
    46741 ggcgtttcag agaaccggtg aaccctatat ttccggcact ttgatagcga atgaattaaa
    46801 ccttgagcca atacaggtca gaaaagacct ctccattact ggtatagtag gcaaacccaa
    46861 gaaaggctat ccagtagatc agctaattgc agccattgaa cactatctag cttgggatgt
    46921 cgaacaaaag gcaatgattg tgggtgcagg gaatctggga tctgctctaa ccggctatca
    46981 agaattcaga aatcatggcc ttcatatagt cgctgccttc gacagcgatc ccttgaaggt
    47041 gggcaagaat atccatcaga ttcatgtata ccagatgaat cagcttagag agattgcaga
    47101 cgaaatgaag ataactcttg cgattctcac cgtcccctct ccttatgcgc agtcaagctg
    47161 tgatgcgatt gtcgacgctg gtataaaggc catctggaat tttaccaatg tgaaactcag
    47221 agtaccggat gatgtgattg ttcagagaga agatttgtct tctggatacg caatactctc
    47281 ggttatgatg cgcacaaaag ccagactttc ctaagccaga attccatcct tgagattatg
    47341 agaatattct taatttcagg ctcgcgctgt tccggtaaaa cctcattttg caattccctc
    47401 atcaagtcag cagaggataa aggcatcgtt tgtaggggct gcattgagat ttcgcaacgt
    47461 tcagagaatg gaatacctca tagcatagac ctttacaatg tgtcaaatgg agatactttc
    47521 ctagcagcct cgcgtccaat agacagaggg gatgttccat ttgaatactc tcccgatgca
    47581 ttcgcacata taagaaaaag ttacattcag actgttgaat cggatattat ttcgcaacat
    47641 cctcaaaaca tacccgacaa aaaaagagaa atttgtttga tagacgaaat cggccccctc
    47701 gagcttgaac atagaggtgg ccatctaagc cttctagaat ttgtgcttgg cacaaagagc
    47761 cctgacgctc ttgtgctaac tgtaagaact ggcttgaaat attctcttat gtctttcctt
    47821 attaatcatg gaatttcggc agatcatatc tatgatctcg agctggaaaa actcacaata
    47881 tcctctgcct gcatctatgt tctttctttt cttgagtagc tctcaagccc tatccagaag
    47941 tcatatcgga ttaacgcaag gccttttagg catatcccag gagatgcaga agcctggatt
    48001 tctgcatttt caacccgatg ccatgttctt gctatattgt cttcccaaaa tcgacaagat
    48061 cagagtattg ggcttcacta atccttccat tctctttcag cgcttcaaag agttcgttta
    48121 catgcataaa tgcatgcagt cttacccctt cccgctccat atccaccctt ccctcactgc
    48181 ctctctctat aagtacaacc aaatcgcgca cctctagccc ttcagatttc aatatatctt
    48241 tagcttctat cttgcttgca ccggttgtta taagatcatc tagcaaaaga actcgatctc
    48301 ctttggcata tctgccttcg atgcgctttc ccgtaccatg atccttggca ggcattcgag
    48361 gccagatcat aggcttgcca agttcaagcg atgctacagt ggcgagaggc aggccagcgg
    48421 agggaattcc gcctatgata tcgaaatcac aagatgatgc gagatgtgca taagctcttc
    48481 ctgcaatcct catagcttct gggtccgata ttagattccg aaggtcgata tagaaagggc
    48541 ttttcttgcc actcttaagc acaaattcgc ccaatctgaa gcattcagta gaaagcaaga
    48601 catcgacaaa ttcgcgtttt agggatgcga cttcatcttc agccttctta gacggctttg
    48661 cggttttgac tgcggcttta aataaaaaag cgtctcttgc tcttcttagg acatctcgaa
    48721 gctctagtgc agcatcatca ggcctttgtg cttccgaaat agaacgcgct gcagcaacaa
    48781 gtataccttt gccgtcgctt cttgcgcccg cggcaaaagc aagatcggct cttcctcctt
    48841 gtgcaccaat accagggctt aaaaaccaag ctgaaggagc aacattcctg actttctgaa
    48901 gcgcttccag atcattacct gctacaacca gtccaacatc gaccgataaa tcagagcatt
    48961 tctgagctac cattgaatag agagggcttt tgttaacagt gatatcctgg aggaatccgg
    49021 ctccgggatt gctagttctg cataacacaa aaattcctct atcgctccac cgcaggaatg
    49081 gctctagggt atcaagaccc aaataaggat tcaatgtgac cgcatcggcc ttgagctctc
    49141 cgaaaattgc ccgagcataa gcttctgccg tgtttgcaat atctcctctc tttgcatcta
    49201 tgataattgg aatttcttcg ggaatctggg ctagagtctt gtatagagct gacatgccag
    49261 cattacccat ggcttcgtag aaagcaatat tgggcttgaa ggctaatgca taagatgcgg
    49321 tggcctcaat gattctcaaa ttctgctcaa gcgcacatgc agcgcattca agactacctc
    49381 ttttctctat ctctgaattt gaaaacgcag gatcgagacc aatgcataga aaggtatcca
    49441 ttttatctgc tctttcttcg atgtattcga aaaagcccat atccatctat cccctataaa
    49501 agtgaaaaag ataatataca atcgaatcgg tgatatagta taattgctta aaaaggaaag
    49561 tagaaggagg ttatgatgaa aaaagcgacc ttattattcg ctcttgttct tttctctttt
    49621 cctatttttg cgcagcaagg aatgaatcct tcatttggca taatagctaa ccttgggacg
    49681 gaccttttgc cagatcctgg cgacccttcg aagttcgaaa gctggtctaa aataggtatg
    49741 cagcccgagt tcagtattgg taaatttggc attgggctcg atctattgtt tagattcaag
    49801 cttggcactg gaagcaatac aagtctagaa atctatgagc ctgactggat tcctcagcaa
    49861 ggccagaaca tttttgatgt gtatcttcct aagattctat atatccgcta tggtcaacaa
    49921 tgggaagatc cgttttatgt aaaaatgggc tcgatctccg attttagcct tggtaatggt
    49981 cttattgtcg acaattattc caacatgcga tttcttcctc agcggcgcgt atttggcatg
    50041 cagctgggaa tcgatggtag cctttttggt tttccttatg tcggattaga ggtactcact
    50101 ggaaatgtct ctaagttcga tgtaataggg ggcaggatct atgttaggcc acttgctttt
    50161 atggaagaga gcctttttgg aagattgcag ctggcagcaa ctggcgtatt cgatagagat
    50221 ccattgctat atactggaac tccctatctg gctcagcaga caagactgat ctatgtagtt
    50281 ggagccgata taaccttgcc tcttatccaa agccccgcat tttcacttac tccctttgga
    50341 gaaggggcat atgagatgaa caatgccatc ggagctatcg cgggcataaa ggggcgcgcc
    50401 tttgacattc tctcctatct cgctcaattt agatatctcc aggaaggctt catcccatct
    50461 tattttgacg caaattatga tatctacaga gccgatcgct ttgtatatat tgagactaat
    50521 cccgcaaaga ataattttat tcctggctgg ctagcatctc ttggtcttga tctctttaaa
    50581 tccgcattga gctttggcgt tatattggat gctccattca aagaactccc ctcgcctgca
    50641 acggacaacc ctgctgatta tgcacatata aagggtagcc taaatctggc ccgtggcctt
    50701 atacccaatg ttaacatcgc tgctagatat gagaaattct tccttggcag aaagagcaat
    50761 gatgtcctta aagaccttat cgattttgag gattcttcta ttggtatgac cataagctat
    50821 aaagcaggca cggctatcgt gtctatggat tatgcttata tctggaatcc aacaaagaat
    50881 agcggggcgg gtgcttttga tgtggtttct agcatgtctc taggatttga attgtgagct
    50941 tttgaggcca atgtctccag tgtaagggcg gtggtcttag aaggacgccg cctttgtttt
    51001 tttacagaaa accattatca tacgaaaaat gggaaatata agaatcctac cacccgaaac
    51061 ctctcgccgc atagctgcag gagaagtgat agatcggcca tcttcagcac tacgagagat
    51121 tatcgacaat tcaatagatg cagatgcgca taatatttcg gtctctatag ctcaaggagg
    51181 catagaggag ataaccgttg ccgatgatgg ctggggaatg tcgaaagaag atctcttgct
    51241 aagcatatcc gaacacgcaa cttccaaaat ctacggaccg gacgatatct tgaaagcgaa
    51301 aactcttgga ttcagaggag aagcgctcgc atccatagct gcggtagctc gattggaaat
    51361 agtaagcaag ctaaggggat ctaatgaagg ctggcgatta tccagctctc cattaaagga
    51421 acctgtaatc gagccgtttg cctgcaagga ggggactcga ttgaccatga gggggctctt
    51481 cgaatcctat ccagcaagac gtcagtttct aaaaagacct cagagcgaag cctttctgtg
    51541 tcgttctacc ttcatcgagc gcgcggtttc tcatcctgac atttgctttc gctgggcagt
    51601 gtcgagcgat atggatgtta tgattgcgtc ttctttgaag gatcgcattc ttcgctgcta
    51661 caccgaactt tctcgtttta cactgtttga agcaatggct gagacatcgg agcttcaaat
    51721 gcattgtatc tacgctgatg tggccaatta taggagagac cgaaaattcc ttcaggtgtt
    51781 cgtgaataaa agaagagtcc cggaatggaa cctgctcagc ctgatggaat atgagtttgg
    51841 caaatatctc cccggggggg cgcatgctat tgcctttctt tttatcgaaa tagatccttc
    51901 gcttgcggat tttaacatac atccagctaa gaaggaggtc cggctaaaaa agctggcgga
    51961 attaaggacc gtacttcatg aactcttatc tcacgaatta gttcatagat atggaggcat
    52021 gactcaggct tttgaaacgg caatttaccc cgaattcaaa gagtttgttt tttcaactaa
    52081 agaaaaacaa atcgcgccgg gcttagcgga tcaaccgcag gttttgcatt caacgaaata
    52141 tgacagggaa atgggaagag aattcacaaa ttgttcatct aattcatact ttgtccgtcg
    52201 agactccaca cagcttccag atgatttctg gcacagaata aaagacgata atctatccac
    52261 ccttcgatac ataggaaaag ggccaggacc atttctaatc tttgagctat ccgatgcgct
    52321 ctttatacta gatcagcatg cagcccatga gcgaattcta tacaacaaaa tcaaatcaac
    52381 tagaggagag agtcagttgc tccttgttcc ttatctgctg gaaccttatg agaatgagca
    52441 ttacttggaa gaatcaaaag ataatctgga atttgttggt tatggaataa caagagacaa
    52501 gaatacctgg attgttgaag ctgttccagc cattgcggct tcggaggctc tcgaagcgct
    52561 tgttgaatgg ctttctcagc cgtgctttga aacctccccc atagatgctc ttattgcaag
    52621 cctggcatgc aaggccgcga taaaagacgg agacagaatg gatgaaatct cagccctaaa
    52681 gcttattcaa gaatccctgg aattgccaga gcctcgctgt cctcatgggc gtccaatata
    52741 tcttgcattt ccaagacagc agctctattc aatgtttggg agaactatcg gatgataaga
    52801 ccggtccgaa tattatgtgt cgcggacgaa gtggatctac ttgtctatag tgtgcagatc
    52861 tgcgaacgct tttcggatgt cgatctgata ttgtcggctg gagatctgcc cgatggctat
    52921 ctcgaatata tagcaagcat gctgaacaag ccgctaatct ctgtagcagg aaatcatgat
    52981 aaatctaaac aggatattga gaatgctggc attcattacc caaggaatca gacagcgaat
    53041 tttggtcaaa tcagctttag aatagagaaa gaaagtggta tatccattct tggcctgcca
    53101 ggttcgattc gttacaacaa aggtcagaat caatataccg atgcatggat gacatttcgt
    53161 ataatattga tgctcccaag gctattgttg agacgcctgc tatggggaag atcagtagac
    53221 atcattcttg cacattctcc tccgcgcggt attcacgatg gtggagatcc cgcccataag
    53281 ggattctctg cttatcgatg gcttattcga cttgccaagc cttattattt cgttcatgga
    53341 catgtgcacg tatacgacat gcaagcgctc cgcgaaatcg attattatga tacctctgtt
    53401 gtcaatgtgt acgggcatcg agtcataact ctgccaaggg aggataaaaa tggccgatga
    53461 tctccacgct cttgccgaat cagatttcta taaggcgaga agtaaggcta ttagcgcatc
    53521 aatattcgga atgctaaggc cttcgatgtc cgatttaatg cccttcgatc aagctaagga
    53581 gttgctcaag cccagagctg agacatatag aggggttact gctgtcccta tcgatagaat
    53641 cgttggaagc gagggacgct atagagactt caatcgtttc tttttcccaa agaaagagca
    53701 tctaaaagct cgttggacaa ctatcgatga attgcaatat aaggatgtga gccttcctcc
    53761 tgtggtattg tatgaaatgg gtggagtctt ctttgtccgc gatggcaatc atagagtatc
    53821 ggtcgctaga gctaagaaac aggagttcat agatgccgag gtaatatcgc ttcattcgga
    53881 gattcaactc gatccagaaa tgacaatcga agatataaag aaagctgtta tcgaatatga
    53941 aaaaaaacaa ttctacaggg agaccaatta tccaaatatt gtcggggcgg atgatcttaa
    54001 ctttagcgaa cctggtagat tcgatagtat aaaagaacac gtccttgtac acaaatactt
    54061 cttgaatcaa catctacctg aagaaatccc ttttcatatt gctctatatt cttggcatga
    54121 taatgtctac cagccaatat ctcaatctat tgaagcagag aatattctat cgctatttcc
    54181 tggaaggact gtatctgatt tatacctttt tcttgttgct cactgggatg aactcaaacg
    54241 caaatcgggc ggctatgttg ctatagacga agctgcagag agctttaaga acgagataaa
    54301 acgaaatcga aatagctctt tcgagcatgc ttgcagaatc tttggatcga tttcaaaaaa
    54361 agtagagaat tttattgcaa aattgtaact tttggaatga tttcgtatta taataaatga
    54421 agagcaagtt gatgtagctt cttcatgcag cctccattgt attagccgtc ggatatccct
    54481 ccttatccgg cggcattttt tatcatgaat tgacttttta tacttatttc tatattgtta
    54541 ctccggtttt tgagaaaagg agtcgttatg gcacaagttt ctaaaaatgc tcgcacaggc
    54601 tcttcaactc aacgcttcta ttgttcttgc ggcggtgaaa taaagatgaa aaccatcttt
    54661 gaaaacagta aggtcagaac tatagctgaa tgcgaaaaat gcaaaagagt tgagagacgc
    54721 ccaagcgact tcaagtgatc tttaaatgat ccgaaaagaa gataaaaggc cccagcatat
    54781 tgccggggct ttttatttat atgcattgtc ctgcaaaatg ttatacgaag atacatcatt
    54841 gtagccgccg aagctcaagg attcgttctt cgatcaaacg tcgcgcatcg atgagcatgt
    54901 tttcgatatc atatttgttg cgtggagctt cgtatatctt gactatttcg acaatataag
    54961 gagttttgcc aaataactta agaaaatcca tagcatttgc tactcgccag ccgccttcca
    55021 gagctatagc ggcaacagag agtcttccac cagactcaag caattttctg acacctgcgg
    55081 tgtgaaattc tcctacatgt ccatttctag accttgttcc ctcaggaaaa atcacagggc
    55141 aagcaccctc ctttgcgcat atctttgcca tttttgacac ttctcgcatt gcatcaagag
    55201 gagctccttt tctctttatc aggcaatgcc ctccttttct caacgtaaag ctcacaaagg
    55261 gaataccata ttgaagctcc ttcttggcga caaagcgagg ctttcgttca attcctaaga
    55321 aagctatgag caccaagata tccagtagac tctggtgatt cgaaattacg agacatcgaa
    55381 gaggaatttc aagccgctcg ggattatgca ccctaatcct gaatccacgg aagaccctga
    55441 ataacccaag gataatcttt gcaccaattt gctcatactt agcacaaaga tgaaagcgct
    55501 catatctatc tgcaaataga cacctcagcg caagaatata ctccaggaga agcaacatgg
    55561 agactatgat caagactacg ataccattga ttagcattat cctaaatctt tatcctattt
    55621 tgcctaggaa tgcaattcct tcaaaaaagt attgacattg atgctaggta tctgctagct
    55681 ttagcacaga attttgatca acccaagatt tgtattaagc aatgctatcc ggaggatttc
    55741 cattgagtac aactaacttg tctgcagaaa agcgccagag gcaaaatgaa aagcgcaggc
    55801 ttgctaataa aagtacaaag acttctataa aatccgcagc caaaaaagtg gtaatggcgt
    55861 cagaaaagaa agatactgaa gcggcaaaac aggctctatc agagatgatc aagcggattg
    55921 attcagcagc cagaaaggga ataataaaga agaacactgc agccaggaaa aaatcccgta
    55981 tgcagcgact tgtaaacaag atagatcaat aaatcagaga gtatagtacc ttcaggaagg
    56041 agcagagaac gatatggccg aaaaattgac aaaagctgaa ttgatagatg cgctgtatga
    56101 ttcattgtcg tcgacaagcc gcactacaag aaaagaaatc catgagctta tcgacggttt
    56161 atttgctgag ataaaaggcg caattcttga tggcaagata gtcgaactga gaggttttgg
    56221 cactttcgag gtaaaactca gaaaaggcag atcaaaggct agaaatccaa agacaggcga
    56281 aatcgtctct gtaacagatc acggcgtagc cacctttagg cccggcagag agctcaaaag
    56341 agccgcatgg gaaatggata tcgagcgtat ccctaaatca ttcagaaaga gagtttaatt
    56401 gctgtccatt tttgctaaat agggatattg ttaatgatgg tagccttcta tgtctatcat
    56461 gaaaaagtca aatttggaac cttcggaacc atacgcaacg catatcgggc ctgcgctaaa
    56521 actgaatgga attctacgtt ctaatcagcc ccttgacttg cattgtactt ttcatggttc
    56581 aatagaagga accttcatta agatttccaa gaatgccata atagaagctg atctattaaa
    56641 agcccatcaa gttgttattg ccggacaatt taaaggatct atgagcgttt ctgaagaaat
    56701 aataattctt cctaatgcta atgtttcgag cgatattaaa gtggcatgcc tccatattat
    56761 tgaaggttcg caatttgaag gcaaaatcga gatggctggg ttttatccag atcataatag
    56821 cccgatagta gatagaggac attgacatcg ctccattgtc acactatatt gaaattgtct
    56881 atcgcatatt tgcggcgcaa aatgccgcac ttgctccaaa aagcatacct ttcgatttga
    56941 ctcaatgcgc cgctcaagaa tccatataaa gtagacgggg agccataaag atgcttggca
    57001 ccgttttgat cgtttaaaat cagggagatt ctcgatggca attcacaata gaaagaagat
    57061 agattcatcc cagacaacat cttcgaataa cgatgagaat aattccgcgg agccaagaga
    57121 ggaaatagcc atgaatcaga gtcagcccga agagcaaccc gtcttaaaaa acgagtcact
    57181 tttcgaagaa cagactgaag atctcgttca agacatacaa aggtcattga acaatgtaca
    57241 tgtcgccgca cctgtaaaga atcagcaaca aaaaacccga aagccagttg ctaaaatccg
    57301 tttaaagaag aagcccactc aaaaaacagg cctcctggag gaaactcaag tacctgaatc
    57361 gacaacaact tccgaagtaa tcgaagaaca acccaatgga ttcgttgttg cagctatgcc
    57421 gctcgatgaa catcaaaatc agagactgtc ggagttgccc aagccggagc cagaaaaccg
    57481 tacagaacag agagtagaat ccagagctcg cttattgatc aatgatcttt caaagatggg
    57541 tataatcgag ttgagagaat taggggccaa atacggtata aatcatgatt tgctcatcac
    57601 actaaaaaaa caagaactca tttttaatat tctgaaaggc cataccgagc acggcggcat
    57661 aatatatgcc tatggttcgc ttgaaattct ccccgatggc tatggattct tgcgctctcc
    57721 tcaaaactct tatttgcctg gcacagatga catttacatt tctccttcgc aaatcaggct
    57781 ctttaactta aagactggcg acactgtcta tggtcagatc aggagcccta aggaaggcga
    57841 gcggtttttt gccatgctcc gtatcgaaca gatcaacttc gatgatccat cggtggctca
    57901 aaatagaatt ccctttgaga atcttacccc tctctacccc acggaaaaat tggatctgga
    57961 aaccagcacc gaagaaatct ctactagaat catcaatctt ttttgtccaa taggaaaggg
    58021 acagcgaagc ctcatcgtat ctccgcctcg tacaggaaag accattcttt tgcaaaagat
    58081 tgccaatgct ataacggcca accatcctga agtctatctc attgtcctcc ttatcgatga
    58141 gcggccagaa gaggtcacag atatggaacg aaccgtaaag gctgaagtaa tctcttccac
    58201 cttcgatgaa caagctaccc gccatgttca agtagctgag atggtgctgg agaaggccaa
    58261 gcgtcttgtt gaacacaaaa gagacgtagt aattttgctc gattcgatta caaggctagc
    58321 acgagcctat aaccaaactg tccccacttc aggcaagata ctgtctggtg gtgtcgattc
    58381 gaatgctcta cacaagccca agagattctt cggagcagca cgaaatatag aacaaggcgg
    58441 ttcgctgact atcatagcca ccgcccttat cgataccggt tctagaatgg acgaagtaat
    58501 ctttgaagag ttcaagggaa caggaaacat ggagatcaac ctcgatagac gcttgtctga
    58561 tcgacgtctc taccctgcta ttaacattaa aaaatctgga acaagaaaag aagagctcct
    58621 gatctccgaa gaagagcttc aaaagatctg ggttctgcgt aaagtaataa atccgatgga
    58681 tgatgctgaa ataatcgaac tgctcatcga tcgcatgact aagacaaaga acaacgacgc
    58741 atttcttaaa tcgatgaaca cgccttattc gagtatggaa tagccttacg tcgtattgac
    58801 tagagttgat tgattttata tattgaatcg ccatatctgg ctgatcttct tacagtagtt
    58861 ttaccagggc tctatggagg aataaatgaa aaagggaata catccaaaat atgatatcac
    58921 aacaataact tgcgcatgtg ggaatgtaat cgagacccgt tctacagcta agaacataca
    58981 agttgaaatc tgttctgcct gtcatccatt ctttaccggc aaacagaaac ttgtcgacac
    59041 tgcggggcgc atcgagcgct ttaacaagaa atatgggata aagactaacg aaaacaaata
    59101 atagcttgag actcagatgc ataaaggctg ccatcgaaaa aaggcggcct tttttatttt
    59161 caaagctaag agcgagcttt ctcagtctct acagccaagg ctgagggtag ctaacatcgt
    59221 tgccattctt tgcgccgcct tgcctctatg cgaaattcta ttcttttcat cagcggatag
    59281 ttctgcgact gtctttccat actgcggaag gtagacaatc ggatcataac caaaaccgcc
    59341 cttcccgctt ggacttccgg caatttcgcc ttctagagtc tcctggacac acataaaacg
    59401 ctgctttcca taatagaaaa caagacaaca aacaaaagca cagttcctat tggaaacacc
    59461 cgcaagttcg gagagcaaaa ggctattctt ctctgaagct gcaagaagct ctttgccatt
    59521 agaaccatac ctggcggatc ctatgccggg ctttccgccc agggcttcaa cgcagagccc
    59581 tgaatcatcg gccatagatg gtattcctaa tagctcataa aggcttcttg ctttaatata
    59641 agcattttca aaaaatgtct tcccattttc ttcggggcag aattcattga ttcccagatc
    59701 tgaaggcatt agtagctcgt ggcatggaaa caggggctta agttcaacga tcttatgcaa
    59761 gttgtttgtg gcgacgacaa atctcatagg gtttttttag cctagttttc tataatcttc
    59821 aagactcctg cctgaatcga catgttttct gagataaaaa agtcctgagt caatgctacc
    59881 cttcggaacc ccgagtattt gcgaaattga ttcattggag acagccgatc tgcgatggca
    59941 gcgcttttcc aaaagcctgg catagcgctc tcgacatcgt tttagagagc atagaatata
    60001 ttctttctct tcattaagaa ggcaggtctc gagtctgaat tccaacacgc ggaggcgaat
    60061 ccatagtgca tgcaatttga tattcatttg ctctgcttta ttccagcctc tctcggttct
    60121 tctttttgtc aattcgatta gattgaaaag gtattctctg ggtataccca gagaaagact
    60181 gcatctgctc aagagttcct catctatctc ccaagcgcat ttcattgtta gaaaaagcag
    60241 tctcttgtga gcagggctca atttatccag aattactttg ggaggaagat ttaaaattcc
    60301 tttctctgtg gttgcatcat ttattgtttc ataatatgtc cacggttcaa ccggagattc
    60361 aattgcgagt tcttctgtga aattggaata tatataagca gtttcacaaa gactgagatt
    60421 ataattttga cgatgtacac tctttgcgac atatcgcata ctgctcagca gatatgcatc
    60481 ttttgttctg ctttcatttt cagcccgttc aagtataagc tcaatgcggt tgcgataatt
    60541 attgagcgca tcggatattt catcctttgt aaaaaagcca tagcgctttg gatgttcata
    60601 catcttgcgg acaagatcga ggtaggtttg agaatccatt cttgagcctc cgattctaat
    60661 cagcaaacaa tgtgccaaat ttgaaaacta agcatagatg aaagagcaat ttttatttta
    60721 ctatttaatc aggaattatt tgatattaca aaactcgatt gaaaacaatt caagcttgaa
    60781 atatgggatg gaaagcaagt tcttgattat tgctgcgcag aactcattta tagaaggcaa
    60841 aaactcggta atactattga ctggcgaaaa taatagttaa atctgatgat attgctttct
    60901 caggcaaagg acttgccttt tcatacaaag ggcgcttgcg cagggtattt ccccgtcctt
    60961 ctcattggaa agaatacact ttccctgaaa aaggacaatt agcaatgagc gcttaattat
    61021 gcttatttcg gcacaagcag gttgtctatc ctgccttcac gcaattcagt ctctattttt
    61081 cgatttcttc gtctctgaaa acgtccccaa atactctgac tagcgtgtgg acgacctcat
    61141 ccaccttgcc attgatagaa aggccagcgg caagccgatg cccaccacct ccatactgtt
    61201 cggcaattct ggcaacattg atggaattct tagacctaag accaacggta cacatttcct
    61261 cggtctcttg tttgactaca aaacagacct cgcagtcgac tatgcccatc attagctgat
    61321 atagcaaatc ggagtctctg ctggacatgc catatctctg ttgatcttct atggtaaccc
    61381 aagaaactag aagtctgcct tcgtagtatg gctgaatacg aagcagaagc tcaccaagca
    61441 tgcgccgaga agccagagtc cttccgccat tgattgccat atacacacgc ttaggtgaaa
    61501 cgccggcttc tacgagcctt gcggctattt taaggctctc acttccatgt tcatccaaat
    61561 gtcgaaaaaa gcctgtatct gtgcataagc caaaaaaaag tagctctgct tcttctctgg
    61621 tcgggctatg acccattgcc tccataagaa gcaaaatcat agccgatacg gccgaagccg
    61681 attcgtccaa ccaatcatat gggcccggag cactcgatgt cgaatgatga tcaatgaatg
    61741 aagcgggaat attgggcatc ctctcggcga cacttcctat tctttccatc gatgagcaat
    61801 ccagcacaat tgcagccgtt ctctcaaaat ccctttcgct tgggacctcg cttttgaagc
    61861 gcgcctcaaa ccggagtatt tcgatcctat tgaatggccc ggaagacaga acatgaacgc
    61921 gctttcccat gctctggagc atagaagcaa gtgccagttg ggaaccgata cagtcgccat
    61981 caggttcgcg atgacccagt atataaaaac aatcgtgatg atttatgaag tctataagta
    62041 tctttggaac ttgtctcata gcagatatta ttgattcaca tcccagatgc tacagcgtag
    62101 caaggattat ggcctttatg gtgtgttttc tattttctgc ctcttcgaac acctttgatt
    62161 gaggcccttc gatgacatcg aatgtcactt cctccccttt cactgccggt agacaatgca
    62221 agaatatgca ggccttattt ccggtctttg ccataagttc agcatttacc tgatatgggg
    62281 agagcattct gattctttcg gctttgaggg cttcttcccc cattgaaacc cagacatcag
    62341 tatatatagc atcagcccca ttgaccccac tctctatctc atccgtgatt attatggtag
    62401 aacccgatgt cttagcaaag ccctcgcaga ggttctttag ctcattatca ggccagagct
    62461 cgcgtggcga tacaatggtg aaatgcatgc ccagttttgc gctgattacc atcagcgagc
    62521 gagctacgtt gttgcgtccg tcaccgataa aacagagacg ctttccggcg caattgccta
    62581 aattttcctc gatagtcaag acatcggcaa gagcttgtgt cggatgaaat aaatctgtca
    62641 ggccgttata aacgggcaca cctgagtatt tagcaagaat ctcgacatat tcttgtttat
    62701 agccacgaaa ctcgatggca tcaaacatgc ggccaagcac gcgagccgta tcttcgatag
    62761 attccttggc tccaagctgg atgtcctgat tagagagaaa gacgggatgg cctccttctt
    62821 ctccaaaagc tgtctcgaag gcacagcgag tacgggtaga gcgtttctca aaaaggagag
    62881 ccatggtctt acccttaaag cgctgcttat gcatgccttt ttttgcttct tctttgactt
    62941 ttttcgataa acttaaaaaa taccttattt cttctggtgt ataatcgatc catgtgagga
    63001 gcgatcttcc tttcagaata gatggattca cgagtcctcc ctcttattgc tattaaatag
    63061 agccgccata tactatatgg tgaattgaga aaggtcaagt ttgcgccagg agagagtaat
    63121 gagcaatgaa ctggaaatat ccctaaaaga aataaatgaa tctattcccc ttcctcagtt
    63181 gctagaagaa ttatacattc gtcttgttcc gatatggcct gacactcgcc ctcttttgca
    63241 ttataacaat tgctttgagc ttcttatatc cgtgattctc agtgctcaga ccacggatga
    63301 tcaggtcaat attgtaacga aagagctttt tcatcgcttt ccgaattccg agagccttgc
    63361 tgccgcagat atacatgagg tagaaaatat catttaccct ctgggctttt atcatgtgaa
    63421 agcaaagcat ataatcgaga ccgcaaaggt gctccaaggg cgattcaacg ggcaaattcc
    63481 atctaatttc gaagaaatga ttgaactacc tggcgtagga agaaaatctg caaatctgct
    63541 tatagcagcc tgttatgaaa agcctggtat cattgtggat acacatgtgc ttagagtgct
    63601 ctttaggctc ggggtatctg caaagaaaga agcgactctt atcgaatcca tcattcgagc
    63661 gaaccttccg ccagaaaagc atacagaatt ctcgtatgct gtgaataggc acggtaagtt
    63721 cacttgtact gcacgcaagc cagcctgtac agagcaaaaa aagcactgtc ccattgacga
    63781 tatctgccca aagataggat tatccgcttt attgaagcaa aggcgttgaa tagagcgtct
    63841 aataattgaa agctaaaaat gttcaaaccg gatctcgttt ctgaatctga agaccctatt
    63901 ggaattctag ggcgcgatag atttcatatc ccacatttga cgcctctcca gcgctttgtg
    63961 attgcgaata tattggatga gactaaaggc tccacagtct tgaccagaaa tctaagttcg
    64021 gatcctatag acgatgacgc agtctcaaac ctgaataaag aaaatgaatt ccaagaaaga
    64081 caggaaacct atcgtcagct tatcctattc ccgactggat ttggcaaatc agtctgcttt
    64141 caactaccct cgctgctttt ggatggactg actattgttg tctatcctct tcttgctctt
    64201 atgaatgacc aaaagcgccg actcgatgag gccaatattt cctgcgctct ttttcgcgga
    64261 ggccttagcc aagaagaatg gaatgaagag gaaaagctgg tctcttcggg aaaagctaat
    64321 atggtcgttg caaatcctga gatcttaagc gcaccaaagc tgcggcgatt cttgaatcga
    64381 ttcaaaattg ctcacttcgt agtcgatgaa gcccactgta taagcgagtg gggagagagt
    64441 tttaggcctt cctaccttgc tctcggtgag actgttgagg caatttcgcc tgggattttg
    64501 agtgctttta ctgcaacagc cggccctcat atcgtgcact cgatcgagaa gagactcttc
    64561 atggataagg attatcgatt agtagattca gaggcagatc gttccaacat tctctatgcg
    64621 gttgtgcata cactttctgt taccaggact ctccgtcaga cccttttgtg ctgtaaaaaa
    64681 ccggctatta ttttcgatcg ttcacgtccg ggaacaagac taaaagccga gctgctcaaa
    64741 gcttctggat tttctcaagc gcgcttttat cacgctggcc tcgatcgagc cgaacgctgc
    64801 aatattgaaa aatggtttca agactcagac gacgcaatct tggtctccac aaatgcctat
    64861 ggcatgggca tggataagaa aaacatcagg actgttgttc acagctcgct gccagaaagt
    64921 gctgaagcat atattcagga agccggtcgt ggaggccgtg acggaaagaa tgctctagct
    64981 gtgctcatag atgatcttgc ggtgcgatat tacagagaaa acctgaagga taagaacatc
    65041 caagacatac agtcgattcg aaaagcttgt tttttgccct atcccacttt gagttcatgc
    65101 cgacgagagt ttctgcttca tctcttgggt gaagaaaaaa ctccagaatg cgggagatgc
    65161 gataattgta tcgaaggatc acatactcag agaacgcaat tcaattcaga tgcctcagat
    65221 atccttccgg aatggaaatt cttgtcaaga gcagaagggt ttattgaggc tatttctttc
    65281 tgtgactcac accatggagt ttttacttcc caagaactag tcgatgcgct ggggcctcat
    65341 ggaaaaggaa aaggggaata tgccggctgt ctatatggat ggctcaagat agaaagagaa
    65401 gaattgatca atgcactatt cttactggat atcctctatg caccgaaaaa gggaacccgg
    65461 aagaacagga ttctacttag ccaaaaaggc aaagctctga gcagacttgc tcgcaacagc
    65521 cttgtatgag cttgaagtgt atcgctatta gcaatatcat gtatgagaaa gaaacacttc
    65581 aacaagaaag actttgaatc gaggggtcat tatggttgtt ttcaaaaacc ttgatacatg
    65641 ccaggccttt gaagccctaa agaagattcc ggccgtcgta ttgcacaaat ctcttgacca
    65701 aaagcgaata tcggactggt ggatccctca cgccgcaggt ttttgctact gctatgcttt
    65761 tgcgccagta gacgattcgg tcatagactg gctcgaagca ctgaccaggg aacaacagct
    65821 catagaaaag tatcgcatac tgacagaagg tgcgattatg aacacaggcg aaaaaaggat
    65881 ggtgctccat cacttggttc gaggacgcct gggcaagcct gttatctgga atggccagga
    65941 tatggaggtt ttctatcgag aagaaaagca tcgattctac gattttgcac ataagattcg
    66001 agaaggcgaa atcctcggca ctacaggcaa ggcttttacg caaattgtac agatcggcat
    66061 aggaggatca gatctaggtc caagagccgc gagcctagcc ctttctcgct gggcagttgc
    66121 agcaggacgg gctaggctca gaccgtggtt tatttcgaat gtagatccag acgatgctga
    66181 ttatgtgcta gcaaacatag atctctcaga gagtctgttt atactcgtct cgaagagtgg
    66241 aaccacacag gaaacgctgg caaacgaaac aattgtgcgc gataggttgc agaaggccgg
    66301 acttaatccg gataagcata tggtggctgt gacaagcact tcgagtccct tagctcataa
    66361 cccccattat cttgatagct tctatataga cgatttcata ggcggacggt attcgacatc
    66421 gagcgcggta ggaggggtga tcatttcgat tgcctatggc cctgaaacct ttgaagagtt
    66481 tttagaagga gcaaatgaag ctgatagatt agcctttgaa ccttccttga gaaagaatcc
    66541 agtactcatg gatgctttga tcggcatata tgaaagaaat atactgggtt ggccagccac
    66601 ggctgttttg ccttattccg aatccctctc tcgcttcccc gcccaccttc aacagctcga
    66661 catggagtcc aacggcaaat cagtaaatcg ggaaggtcat cctatttctt actcaaccgg
    66721 gcctttagta tttggagaac ctggcacaaa tggccaacat tctttctacc agcttctgca
    66781 tcagggatcg gatattattc ctctccaatt cataggtttt ctcagaaatc aactttcgcc
    66841 agatatgatt tctgagggtt cgagttcgcg tcaaaaactc ttggccaacc tcattgctca
    66901 aatccttgca tttgccatcg gcaaaaagga tgaaaatcca agcaaaactt tctcagggaa
    66961 ccgtccctcg acacttctat tggctagaga actcggccca aggcagcttg gcgctcttta
    67021 ttcccatttc gagaataagg ttatgtttca gggttttgtg tggaatctca atagtttcga
    67081 tcaggagggt gtacaactag gaaaaatcct tgcaaaaaag gccttgagcg caatcaccaa
    67141 aggtgaatcc actgatgatg aactattaga tgctttcatt gcttctgctt tgagagctgt
    67201 cgagaataat ggtgattgag tcagttggat aatgggaatc aatgaccaat gggagaaggt
    67261 aaaatgcagt ctatttatga aacggagctt caggagaagg atgaacttga gaatgactgc
    67321 atcgctttta cgggtggagg aacaggaggt catatatacc ctggcttggc ggttatagaa
    67381 gaattgcgcc gccttgggtt taagggaaga attgcatgga taggctccga aaaagaatta
    67441 gatcgcagca ttgttaccgg acaaggtata gagtatcatg cgattccttc gggaaaattt
    67501 cgtagagagc tctcgcttcg caatatctct gatctgggca gggttgcctc ggcttatttc
    67561 agagcgcggg ctcttttaaa agatatcggt ccatcgatcc tattttccaa aggaggctat
    67621 gtgagcgttc ctccttgctg ggctgcatcc tccctgggta tccccgtaat tacccatgaa
    67681 tcagatgcat cacctgggct ggcaacccga ctcaacagca agaaagcgtc gcttatcctg
    67741 acaagctggg aggtgacggc tcaatacttt cctgagagtc tcagaaccaa gatcataaga
    67801 gccggaaatc cagtacgtcc tgatatcttg aatggagatc gacagcgcgg actatcaatg
    67861 ctcgaatttg tagctgaaaa gcccgtatta tttgtgctag gtggaagcca gggagcacaa
    67921 gaggtaaacg atatcgtact ttcctcgctc aatctgcttg tacctttggt tcaaatagta
    67981 caccaaacag gtagggctaa tttcgatgaa gtcaaggcga gagttccgga tgatccagcc
    68041 gtgcgcagag cataccgtcc ttttccatac ataggttctg aaatcgcaga catctatgcg
    68101 gcttcgagca tgattgcagg aagggctggt gcaggcacga tatgggaggc agcatcgctt
    68161 ggcaagccta tgatcctcat tcctttatct ggatctggta ctcggggcga ccaagtggaa
    68221 aatgcagctc ttgctgaaca ttcaggcata gcgactgttc tgctgaagcc caatatttca
    68281 tcggatttat ttgtcgatgc agtcaaaaaa tatctgaata tcaagaatta tgccatggca
    68341 gaagaggcat gccgcaattt agtgcacatt gtcaaaaccc cccatattcc tgaaattaca
    68401 gatggtccta gccaaaatcc aatattatca gcggaatata tagctcagct ccttctaaca
    68461 aagatggcag aatgggcaaa ggaatctgca tgagtacaat agactgggtt tttagcggga
    68521 ttgtaataat actcgcagca cgttgctttg ttagaggctt tatccatgag attctatctg
    68581 tagcttccat cgcattggga atccttgctg gcttattgct ttcaaacact gtcatcctgc
    68641 agatattgcc gaagatcaac gccgacaaac ttccttatca agctcagtat atcatcgcct
    68701 ttattctttg cttcatagca gggtttcttc tcatgaagat catagagcgc atgcttcgcc
    68761 aagggctcga ggcatccaac cttgacatac tcgatagaat cctaggcctt attctgggtg
    68821 ttgcggaagg ttttattgtt gtgggtctct ttattgttat tctggagata cagcctattg
    68881 tagacgcaaa ggcaatactc tccaattcgc tctatgtaaa acttttggga ccaatcgtcg
    68941 aacccgccat aggtggaact ctgagcccaa tgctaaagga tatggatatg ccaatcctta
    69001 agcagaagcc caaaggcaaa tgatcatgtt tgagaacctg ctttttcaag aacatgcttt
    69061 agaacagctt gtttcgctgg caaaggaaaa gaaggtgcca cccgcccttc tctttgttgg
    69121 tcctgccgga tccggaaagc tgacagcagc ccttgaatgc gctcgtgttc tttcttgcga
    69181 aaaagaagcg gaatggaatt gcgaatgtac acaatgtcgt tgtcatagaa accttatgca
    69241 tcccgacctt ttgctctttg ggcctagaga attcgcccag gaaaccatcg ttacaggaga
    69301 atatctgatc ggcaagcctg gcctgacttc atattatgcc tttgtgcgct cgataaggaa
    69361 actcctaaaa cgctttgatt cagttctttg gcagggagaa gaagcaagac tatcaaaggc
    69421 tataccatcc atagaaacct gcgaggagct gatacaagag atttctgaac ttgtgttgaa
    69481 gaaaaaaaat cacgccctga ctgtagcggt agaaaatgcc ataaatgcgg cagctggact
    69541 agaatctctg gtaccagatg gtattccagt ttttatgatc agaaacatga tttcatgggc
    69601 agtgcttgcg ccatctgcaa aaaccaaaat gatcataatt caaaatgccg atgccgctaa
    69661 tgaaagtgcg cgaaacgcaa tgctcaagat tctagaagag cctccagaga cagttcgttt
    69721 tattctgact tcatctcgcc gcgctgctgt cattgcaacc attctttcaa gatcaaggct
    69781 catatcgttt gactctcgaa cgggtgatca agcgcgcgaa atcatctctc gtctatttca
    69841 tgtagatgaa gacctgcaaa acattaacag cataagtgaa ttctttcaga ataagagccc
    69901 ttatccacct aaggtcgcca cccgagatgc agccttattt ataggtgctt tgcttggaaa
    69961 aaccatgaaa gagaatcctg gattgagtgg aaaatatgct attgaacttg cctccatggc
    70021 gcaatgtgag aatgtctccg ctctatcgat tcttcgccaa gtatcagaca aaacgaatgc
    70081 ctttggtgcc aagaattcta gattcgctaa ttcgtttctt gttttcatga gggcggtttc
    70141 acagcatctg gcgcatatag ctgctgatcc aagctcgagc ccttctctta tcatgtatgt
    70201 agatcgcctc ttttccaagc tgagggagat gaacatgcaa tatcgtacat tgaatcggtc
    70261 gccagaactc atgcttgaag catttgccga tatacttgga gagcagtatg aaagccgcat
    70321 atgaaaacac tcttaagcga atcgatcgta ttcctccgga gggtctcaaa cagatagtaa
    70381 gaaacctatt agcagagaac tcgatctatg aatctgccct aaattctacg ctcgatggga
    70441 tcatcgtatg cgatctagcc cacaaaccgc tctttataaa tcgctcggca gaaaaaatca
    70501 caagaatcgt atcatgggca caagaagttg tactttggga acagctcgat gatgacaatc
    70561 tggctgattt tatccaagaa gccctttcat ccgaaaagaa tatattgaat cacgaactct
    70621 ctatcagcag gcctggaggg acgaggctgt tatccatcag catatctgcg ctggtatcgg
    70681 aaggaaaaat agcgggcaac ctaattcaca tcgaggacat tacagagaag cgccgccgcg
    70741 aagctcaact ccgtagggca gaaagcttgg ctgctcttac aaccctggcg gctggagtcg
    70801 cgcatgagat aaagaatccc cttgcctcga tccgaattca gcttggcatt attcgacgca
    70861 tactcgaaaa gaattgcgcc aaaaaaacag agcctatctt tcataacatt tctttagttg
    70921 agcaggaaat agatcgactc aattcgattg tagtcgattt tctatttgcc gttcgtccca
    70981 tggatataac gctcattcgt aatcgtgttg aagatgttat caaggaagtt gttgaaataa
    71041 tgggcaatga ggcctctgtc catcatatca atatcgtgtg cgaattcgaa gataatcttc
    71101 ctgaagttat gatagaccct aagcatctca agcaagctct gctcaatatt atcaagaacg
    71161 ctattgcagc catgcctaat ggtggaactc ttactatctc tgccgtgaca aggcacgacg
    71221 aacttcaaat atctatttcc gatacaggca tcggaattcc tgaagaactc cttgccaaga
    71281 tattcgaacc ttattttact acaaaggaaa gcggtacagg cctagggctt acgatcacat
    71341 tcaagatcat aaaagaacac aatggcgaaa tcaccgtgga atccaagcca tcaaaaggat
    71401 caacctttac aatccatttg cctatacctc aacctgagca gaaatccctg ccaccttggg
    71461 aagatggtct acaaattgaa ggctcggaga gggaggaata gatgcgagca acactgttgg
    71521 ttgtagatga tgagatcaac atacgcgaag gactcgccca gcttttttcc ctggaaggct
    71581 acgatgtaaa aactgcaaag gatggcttag agggaaagat gatcgtcgat aaggaagata
    71641 tcgatgtagt cataaccgat tacaaaatgg gaaggatgag cggtctcgat cttctgcagt
    71701 acattgttag cacacatccg ggcatacctg tgataatact cacggctcat ggtagtgtcg
    71761 acaaggccgt ggaggcaatg agagaaggcg ctttcgattt cattgccaaa ccgccaaata
    71821 ccgatcatct aatcaatctt gtccgccgcg ctctggagac aagagagctc tatcgaacca
    71881 acatcgagct aaaggcagaa gtcgaaagcc agcgagctcg gagctacatc attggtaaag
    71941 ccccctccat aaaacgcatt ttcgacctca tacgcaaagt agccccaacc aaagcaagcg
    72001 tattaataac aggcgaatcg ggcgtcggaa aagaacttgt agccgacgcg attcacaact
    72061 tatcaccacg cagagacaag ccttttataa aggtgcactg cgctgctctc gccgagagtc
    72121 ttctcgaaag cgagctcttc ggacatgaaa aaggcgcttt tacgggtgcg accggcagaa
    72181 aacgaggacg cttcgaaatg gctgatgggg gaacgctttt cctcgatgaa attggcgaaa
    72241 tcaatcagaa tgtgcagatc aagattctgc gtgttcttca agagcgaaaa ttcgaaagag
    72301 ttggcggcga agagactata gaagtcgatg ttagattgat cgctgccacc aaccgagacc
    72361 tcaaaagaga gatcaccgaa ggccgcttta gagaagacct atattatcgc ctgaatgtag
    72421 tcaacatcca tgtgcctcca ttgcgcgaac gcagagaaga tattccgctt ctcgctatgg
    72481 cttttttgag agagtttgca gatgagaatg gcaaacagat cgagggtttc gatccaagag
    72541 tcagacaggc tctctacgcc tacgactggc caggaaatgt cagagagctg agaaattgca
    72601 tcgaaagcgc agtggttatg gcctccggta agctgataac gctcgatgac cttcctccgg
    72661 ggccgcgctc gagcggagaa gctcgcacaa tcagcatacc ggccttttca tctctggaag
    72721 aagccgaacg gattatcatt tctgaaacgc ttgctcttgt aggaggaaat aagtctaagg
    72781 ctgccgagat acttaaaata ggccgaaaga cgctttatca gaaaatagcg caatatggga
    72841 ttggaaaaca agaagccaac tcttcggtgt cttccaaccc gagagaagaa acagagaatt
    72901 gataagatcc ttgatacaat atgagcatac tggctatatt atgcctcgtt ttttattcga
    72961 tagtttggca cgaaggcgta atatggcctt ggtatggagt tggctcaccc tagattctgt
    73021 tacctcaagt atcttgccta tttcgcgtaa ggtcagattc tcatagtagt ataggaccat
    73081 gacttttttc tccttttcgg gcaactcctg gagagcctca aatatgactc ttcgaatttc
    73141 ctcgcgttcg agcaccaagt ctggattttc aagtcgcgtc gattcgagag aatcttctat
    73201 agtatatccc tccgcttcgg aattaattgg ccagatctca tgcagagaaa gaatcgaaga
    73261 agacgccact ttagacatta tcttcaagag ttctttgcaa tctatcccca tctcctttgc
    73321 tatttcttca tcggtggcgg gtcggccgag ctgactctcc aattcagcga tagcgcgctc
    73381 tatctgcttc gtctttcgac gaatggaacg aggcacccaa tccagttctc tgagatgatc
    73441 atagatggct ccacgtatcc gtgtgaccgc ataagtctta aattttacat tcttgtttgg
    73501 atcgaatttt tctattgcat caaaaagccc aaatgttcca taaccgacaa ggtctcgata
    73561 ttccaccatc gagggcaaag tcacagatac ttttcctgcg acatacttca caaggggggc
    73621 atagcgcatg atgatctcat tgcggatatg gatatcttca gtccgtcggt aaagctccca
    73681 taaacgatct tcttctaatg ttgcatagtc catgatccgc cctttccctt tttccgttaa
    73741 aggatatact tgctcaaatc ctgctgttgg acgtagtcct taaagcgttc gtccacgtat
    73801 gctctatcga tgacaacttt tgtgcttgta tgcttgtcgg catcgaaaga aatgtcttct
    73861 aagagccttt ccattaccgt ctgaaggcgc cgcgcaccta tgttctcgct ttgcctattg
    73921 gctgcggcgg cgatctcggc aattcttgca atagcatccc cattgaactc aagctgtaca
    73981 tcttccgtgc tcaggagttg aatatactgg gttacaagag cgttcttagg ctcggtgagt
    74041 attctcacga aatcatcgcc agtcaaagcg tctaactcta ccctaattgg gaagcgccct
    74101 tgcagctccg gtatgaggtc ctgaggttta gccacagtga aggctccagc tccaatgaat
    74161 aaaatatgag ttgtatcgat tggtccccat tttgtgttga cggtcgtccc ttcgacgatg
    74221 ggtaaaagat ctcgctggac tccttctctt gagacatcta tgcccgaaga gcctctagca
    74281 tcacggttcg caatcttatc catctcatct atgaaaatga tgcccaactc ctgagtgcgt
    74341 cgacgagcct cctggacaac cttctcctga tctacaagat gttctttttc ttcggcttca
    74401 agaataggta aggcttctgc gacactaacc gtctttttct ttttgctacc accaaataag
    74461 ccttgcagcc cgcccaaagc gatattcatc tcttcgaact gcgtcccagc aaagagctca
    74521 atttgtgggc cctgagcggg caccgtaatt tcaatttgtc gcgaatttag tttgccttct
    74581 tttaagagag tgcggaattt ttctttggtt tccattccgg aattaggaat tatcaatgtt
    74641 gcttgagatt gcccaatagg ctcatcagat tttgccaggc ctgggagcaa aatatccaaa
    74701 agtctttctt ctgcgcgcct cttcgcttca gtcgcgactt gggagtccat ttcctctttg
    74761 accatcgata cggctgctgc catcaaatct cggaccatag attcgacatc ccgacctaca
    74821 tagccaactt ccgtgtattt tgtggcttcg atcttaagaa aaggcgcatt acagagcttg
    74881 gcgagccgac gagcaatttc ggtttttccc accccggtgg ggccgatcat aagaatattc
    74941 tttggagcaa tctcttcccg gatttctgga ggaagacatt gtcgccgaat tcgattgcgc
    75001 aatgcaatgg ctacggcctt ttttgctttt tcctggccga cgatataccg gtctagttct
    75061 tcgacgattt tatggggagt aagcgcatct ttctctataa tcatatctcc tccacgataa
    75121 tctgatcatt cgtatatatg catatacttg aggcgatctt taggcttcgt tccgcgatct
    75181 cgcgagcgga gaagctatct gggagcattc ccgcatcctc gcccggggtg gatcttgctg
    75241 cttctttcat tctgcgagaa gcatcgaggt aagcaattgc ggcagcgtat gcatagctgc
    75301 ctcctgaacc tatcgcaatg gcatcttcgc ttggttccac cacatctcct gttccagaaa
    75361 gcaaaaacat cttggattgg tccgcaacca agagcatagc ctccagcctg cgaagtacac
    75421 gatccgtgcg ccagtctttt gccaactcaa ccgaagccct ggtcagatcg ccaccatact
    75481 cctgcacttt ggcttcgaaa tgttcaaata gggtaaaggc gtcagctgta gctcctgcaa
    75541 aaccacacag aactttccca ttatagattg tccgtatttt gcgagcattg ctcttgagca
    75601 cagtgttatt catcgtaacc tggccatcac cggccattgc aacatgtcca tcttttctga
    75661 cagccagtat cgttgttgca tgcagttcca tatttccctc tctttctctt atttggtctt
    75721 tgaaccgtga ggatgcgcct gctcataaac ccgacgcaat cgctccatgt cgacatgagc
    75781 ataaacctgt gtggtcgaga tattcgaatg accgagcatc tcctgtacaa cgcgcatgtc
    75841 agccccagca gctactaaat gagtggcaaa gctatggcga aatgtgtggg gcgtcaggtg
    75901 ttttctgatg ccagctttga gacgtcgatt ttcgactatc ttttcaacac cacgagaact
    75961 caatgggcga cctctcgaat tgacaaagat cctatcatgt tcttcgatgc catattttcg
    76021 aagaattgcc gctctataag gcagatattg gccgagcgct gtattcgctg catcgcatag
    76081 gaatacaaga cgctccttag aacccttgcc ttttactcgg atcgtgcagt tgttcgtgtt
    76141 taatctcgaa agtcgcatac cacatagttc cgaaacgcgg ctgcccgtgg aatacataac
    76201 ctccaaaaga gccctgtccc tcacatccag aaaagtatct ccccctaaac cgtcaagaag
    76261 actattgatc tcttcttcaa aaagaaatga tggaagagtt cttcccaccg taacattttc
    76321 tacttctcgt gcaggatctt ttcctttttt atgaaacttc atccaatatc tataaaagcc
    76381 gcgtaccgca gataaagccc tgttgataga tgatgcagct ctcttctcca tcacaagagt
    76441 accggcaaat gttctaatat cacttgcatc cgcctcatcc aggtcctttc cttcgagaaa
    76501 tatctcgtac ttcgcgagat ctcgcctata tgcttcgact gttctaatcg aaaggcctct
    76561 tacagaagcg agataatcaa ggtagtccaa aagtcgcgca tccatgattc atgactctcc
    76621 gttgttttct ccactctgtt gactcgagtg gagatgatca cagtctggat ttatgcaaag
    76681 tttgcgatga ccggtttttt tgtcgtattt ctcaacaaga aaccagccac attttgggca
    76741 caaagaattg cttggcttat aaagggtttt aaaattgcat tcaggatagt tcgagcagcc
    76801 atagaatttc tttcgcctac ctttgttaga gacacgcgga acaatatcgc ccccgcattt
    76861 tggacaggta gccagcggca ctgatttagt aaatgtgcat tctggaaatc ctgaacaaga
    76921 tataaaatac ccaaacctgc ctagcttttt tactagagga cggccacaga tcggacactt
    76981 ctcatcggtc ttctcgtcca atgcaccatg catatcttcc aggacgttca aggcgttttc
    77041 gactttatcc accaatggga aatagaactt tttgagctca cttacccaat cgatcttctg
    77101 ctcctcgact ttatcgagca tggtttccat ttccgcagta aagctggtgt tgattacttc
    77161 cggaaagttc tgggaaagaa ggtcatcgat tattctgcca agctgcgttg ggacaagctg
    77221 gcgtttatct ctgtggacat aatatcgctc gatgagagtc tcgattgttg gcgcataggt
    77281 tgaaggcctt ccaattccca gctcctctag ggttcgcaca attgaggcat cggtgtagcg
    77341 cgaaggccct tgcgtgaagt gttgttcgct ttcaattctt tcaacctgga gagtctcacc
    77401 tttctcgaac ggaagatgat ggcttgctct ttcctctttg gaagccgcga ggcgaattac
    77461 cttataaaag ccctcctcta agaagctaga ggctgaagta cgaaaaatac catcaccaat
    77521 taaaatatca gcagtcaagg ttcgaattat tgcaggcttc atttgagagg caacgaattg
    77581 ttcccaaatg agagtataca atttcaactg atctcctttt aggtaacgcc ctgcttcatc
    77641 gggagtaatg tccactcgag tcggccggat tgcttcgtga gcgtcctgag cggcattgct
    77701 gaccgaatat ctaataggcg cagctggggt ttgattggga aagtattttg tgagccattc
    77761 gtatatttca tggagagcgg cctcagaaat tcttgttgaa tccgtcctca tgtatgtaat
    77821 caacccagtc cgctgatgcc ccatatctac cccttcataa agctgctggg cgattttcat
    77881 cgtctttgta ctcgtaaatc caagtctgtt ggatgccgct tgctgcagct tcgatgtcgt
    77941 aaagggaggt tttggcttga ctgtgcgatc gatagattga atatcgctta ccagcgctgg
    78001 ttttcccaaa aacagagcaa gaatattgtt tgcctctgct tcactcttga tcgagggctt
    78061 ctcgccgttg agcaaagcta attcggctcg cacaatgtgc tgatgggcct taagatgggc
    78121 ttcaattgtc cagtattctt cggggataaa ggattctact tctttttctc gatcacagat
    78181 gagtttgaga gcaaccgact gtacacgacc ggcagaaagt cctgatttta cttttttcca
    78241 tagaagcggt gaaagcgtat agccaaccag tctgtctatt atgcgcctgg ctttctgagc
    78301 ttccacgaga ttcatatcta tatcacgcgg attagccatc gcatcctgta cagcttgctt
    78361 tgttatctca ttgaaagtaa ctctgcttat aggtgtatct tttgctctat cttttaggta
    78421 ttttcctatt tgatacgcaa tcgcttctcc ttcacggtct gggtctgctg ccagaatcac
    78481 cgcgctcgag cgctttgctt cttcggttag ttcttttaat ttctcggctt ttccgcgcac
    78541 ggtaatgtat tccggctcga aattatgtgc aacatcgacc gcgatacgcg attttggaag
    78601 atcgataacc tggccattgg aggcaaggac cttgtaccga gaaccaaggt atttctcgat
    78661 tgtacgagcc tttgcaggcg attccactat tacaagagtc ttgagcttgt gagtttcagc
    78721 cgccttagcg gcttcgggag tcttcttttc cttgcttttc tttgctgtca ttcaataacc
    78781 tcttgaaatg caactccttc gattgcggct tgacttggta taaatcatcg ccgtctatat
    78841 tccattatat tcgcaccgga tcatgggaac gagttcagcg ccagctttta ttgccaaagc
    78901 gccatcttcc ttcagagcat ccgctcctga atttcgagaa gatccaaggc aagaggcagc
    78961 gaccgccaca tcccgaccct ctgacagtgc aatttccgca gtaatgagag ctccagattt
    79021 ctctggagcc tctacaacta atgttaattt agaaaatcct gcaagaatgc gatttcgttc
    79081 cggaaaccga tatctatcta gacttacgcc cggcggatat tccgtaacca agcatcctcc
    79141 gcaatcgagg attttcgctg ccaaagctgc atttgaggag ggatagactc gctctggccc
    79201 acagggtaaa acagcgatag tagctgcagc accgctttga attgctccac ggtgagacgc
    79261 ggcatctata ccacgagcaa ttccagacac tatcacaata ccttcatcag ctgtttcttt
    79321 tgccagaagt tgtgcagtat aaagccctct cgatgtcgga cttcttgtcc ccaccattgt
    79381 caccgatgcc tgatttagag cctttgtatc gcctcgcaca aaaagaccaa atggtgctct
    79441 atgtatttcc cttagaagcg gtggataatc tttatcgaca acgcagacaa atctagctcc
    79501 taatcgatct aatatgttga tatccctctc ggcttgttcc catatcttac ccatatccaa
    79561 tggtcgtgca tgcatggaac ggtatagaaa gctttcgatt tcggcaagac ccagggctag
    79621 aaaatcctcc atgtcatgaa tgttctcaag cagcaaaaca cgctcctgcc aactgagaaa
    79681 atgaagtctt tggaaagcaa gtcttagagc aagaggagtt ttttccattg aaggctgaat
    79741 tgctgagata cgatcgagct ctaattggat aagcttgcct attcccttat gctgtttccc
    79801 gagtgtcatc tgtgagaacc ctccatgagg ataagcgcat ctaatctcgt tctccgcttt
    79861 cattttcttg ctttcctgtt tctatgcctt attcttctcc gatttgcgtg gaaaatactc
    79921 cttgtgatac atcatcagcg attcttcatc gatgtgagta tagatctgcg ttgtagctat
    79981 atccgagtgc cctagaagct cctgaacagt cctgagatct gctcctcctg ctaataaatg
    80041 ggtggcaaaa ctatgtctaa atgtatgcac cttggcattg acccctgatt tttcacgtat
    80101 cttgctgaaa cgcttccaga ttcccttgcg agagatgcct ttgccctcct gattaagaaa
    80161 tacaaaatcc gttctctctc gtttttcgag aagaggcctt gcctctgcca aatagcgtga
    80221 gagccaatac ttggcttcgt ctccaaatgg aacaagacgt tcttttttcc tttttcctat
    80281 gactcttagc agtctttctt cgagatagag ttgttgaaat ttaagattag ctgcctcgga
    80341 gatccttagt ccgcaagaat atatgagttc gaaaagcgcc ctatccctaa gcccattagg
    80401 tgaagtaatg tcaatgcatt ccaatacatg ttcaatggaa tcaggatcga gcacatcagg
    80461 cagattccta tcctgctttg gtgtgcgtat ttgtatcgat ggatcgtctg ctctgagttt
    80521 ttcgttgaga caaaagcgat agaatgagtg cagactagct ataatacgag ccatggttgt
    80581 tctttgaagc cctgactgtg acctgttgac gagatatgca agaagatctt cttcaccagc
    80641 atcgagcggc gtttttccat attgaaaaag gaaattctcc agcatttcta tctcacgaag
    80701 atatgcatcc gcagtaagta cggagcgtct ccttgtagcg agtagatagg cttgaaaacg
    80761 ccggagtaga atactcacct tctgcctccg aagtcttcgg gcattaagcg tttattgcga
    80821 agtgctgttg ggatgtcgaa ttcgtcatta tcggattccg gatcgaaact tattctgcca
    80881 aaattatcct taaaagagga tttatcctgg ttctcgcttg cttgatctcg cttttgggct
    80941 ggtttttctt catgcagggg ccattcgcct gggctaagaa attcgcttgg acgaattgat
    81001 cctaaaggca tcgcaatggt gttctttgtt acagtctttt ctcctagttt ttgatgcaag
    81061 gcaccaaaac cagtagcgat caccgtcact ctcacctcat cttttataga actatctgtt
    81121 actacaccga caatgatgtg ggcatcttcg gccgcctttg atgttattag atcgatgact
    81181 tcctgatatt ctattagaga aaagtcctcg ccacatgtga catttacaag gatatttcgc
    81241 gcgccttcga tgcatgcatc atcgagaaga ggattattta tcgctttctc cgaagcttct
    81301 tgggcgcgat tgtctccgct gccgacgccg atacccatta ttgcatcgcc ctgcccttcc
    81361 attacggtcc ggacatcagc aaaatctata ttgatatctc cagcaagtgt tatcaggtca
    81421 gagattccct gcaccccctg tctaagcact tcatcagcaa ttcgaaatgc ttcttttaac
    81481 ggggtttttt tgtctacaac cctaagaaga ttctgattgg gtatgacgat taccgtatcg
    81541 gcagcctgtt ttagcttcga aaaaccttct tctgctattc tgtttattat tttgccttca
    81601 aaagcgaaag gttttgtaac gactgcaacc gtcaatgctc caagatctcg ggctgtctgt
    81661 gcaattatcg gagcagaacc cgtacccgtg cctccaccaa gaccagctgt tacaaagacc
    81721 atgtcggctc cgcggagcgc ttgctcgatc ttatctctgt cttctatggc agccttctct
    81781 ccaatctctg gttttcctcc ggcgccgagc ccttttgtta tttttgatcc tattccaagc
    81841 cttatttccg cttttgcacg acctagatcc tgcaaatcgg tgtttaccgc aataaattgc
    81901 acattcttca accctgccat aatcatgcga tttactgcgt tcgagcctcc gccaccagtt
    81961 ccaatgacct ttatcacagt tgggcttcct ataccattgt cgtcaacaag ttctattgcc
    82021 atattcttcc tcctatacaa gcatcagaaa aacctatctt ttatccattt tatgggagaa
    82081 aatccacctt ctttgccttt tcgtgctttt cttctcgtcg aaggctccgg tgttccaatt
    82141 cgttctgcct caagcaaaac tatgccaaca gccgcagaga atgccggtga acgaaaaccc
    82201 acatcaaggc cgggcaaggc tgtaggtgct cctattctta caggcacccc gaatactgac
    82261 cgcgcacatt cgtctatatg aggcagtaga gctgagcccc ctgtaagaac gactccagcc
    82321 tttatcagtt ctttcgatcc attctttacc agtcgttcat ttaccatttc gaatatttct
    82381 tctacacgtg gctgcacgat cgataccagt tttctgcgtt ctaattccac tggttcgcgt
    82441 gctccgaacc ccggcacaat gacagcctca tccggatcga tagcctcggt ccatgcggct
    82501 ccagattctt tttttaggcg ctcagcgcta tcgatgggaa tactgagcat tatcgatata
    82561 tcgcccgtta tttggcttcc tcccaaggga atggccgatg tatagacggg atcgctttcc
    82621 gaaaaaacca tcagatctgt agtgcctcct cctatatcga ggagaagaca gcctaactca
    82681 cgttcatcct ctgtgagcac tgtgcgcgca cagcccaata ttccgagata gctctgttca
    82741 accttgtaac ctgcccttgt aacgcatttc aacatatttt gtacggttga taccgagccc
    82801 gttatgatat ggacactgca ttcaagccga acacctgtca tgtggagcgg attacgaatt
    82861 cccttttgat cgtccaccgt ataggatcga gggacgacat gaagaatctc gcgatccatt
    82921 gaaattgaga ccgcacgagc agcctcatga acgcgagcga tatcctctgc cgttatttcg
    82981 cggcctcgcc ctgttaccgc tatctgtcca ttggtattga aactctcgat gttcgcaccg
    83041 gaaagagcca gcgagacttg cctaatatct attcccgaca taagctccgc tgcatcaatg
    83101 gcagcgttga tactattcaa agtgccttcg atattgagca ctactccgcg tcgcagccca
    83161 ctcgaaggtg ccgctccaat gccaaagatt tcaaattgtc ccatctcgtt gaatccaccg
    83221 acaatcgctt ttgtaacagt ggttccaata tccagaacta caatacttgt atcagccaga
    83281 gactccctcc tttgcctgat agattacggt gccggaatga aaatctattt cgcttggctg
    83341 gtcacttaaa ccgcgcgcat gcaaaatatc aagaaccacc aatgcattgc atagagcttc
    83401 ggcactgaat ctgagcggta agcgtaccgc cgtcttgaca tgcatgggat aaagcacaat
    83461 ttcgatattg tttgccgaga ttctttccac atgtatttct gaaaacgccc ttagtagttc
    83521 gggtgaatct tgcttgataa ccgctaaatt tgcaaaaagc ggcagtatgg cttccggtag
    83581 ctgctggccc aaactgaact gctcaaatct aatgcctgat accaccggta gctcgggatt
    83641 attggaatcg tctgcgtcaa gagaagcaaa tgctattccc tcgccatcga ccagaatgag
    83701 ttttgaacct tgttcggtgt taatcgttat cgatgctacc gccagacgct ctcggatatc
    83761 gatcgctagt gtggaaggca ataccctata gactttcgcg ccagctattc tagggttttt
    83821 cctaatcgaa cttgctatct cttctatatc tatcgaaaga atattttcat ctgcaatctg
    83881 gcctaaggac tcgattattg agttttctga tatagtctct aagccgctta ccctaactgc
    83941 atcgatcttg gtaagtcgtg gcagcgatat agccgctcca gccgatagaa cacataataa
    84001 ggtaattgca attaggatat gtaagcttct atgtctcttc gaagccatcg atgtcttggc
    84061 aggctttacg gactcactga gtttttcggc ttgtacagat agggcaacag aatcttgtgg
    84121 agcacgcctt atgaatggat taacggaaat ctttgtcgcg gatagggatt gggctttctc
    84181 ttctgttgct cgatgagctt gttctgagga ggtatttatt ctctgtgata tttggatctt
    84241 ctcgcccgca tttattctgg tccccgagct tttcgccgtt gaactgccag ggagggcagt
    84301 ctcacagcgt gtagtggcct ggatgatatc catagaataa ttatcagatt tcgagagaag
    84361 cgaatgcgtt cccttttttc tcagggcttt tgtctcattg ccctgttcct gcaatctgac
    84421 atcagtcata tgccgcccct tcttttctta aggctgctct catgcctgtc tcagtttgac
    84481 cacttcgaga gagattgatg agaatgccac aagaaattgc gctcataagt agtgccgagc
    84541 caccagctga aaagaaagga agggctagac cggtagttgg catcactcca gtaacaacag
    84601 caagattcag aagaacctgc aaagataaga gagttatcat tccaaagcct gcaagcctag
    84661 aaaagtaatc tgatccaata agtattctgc ccgcccgtat cagtatgaag gaccaaagcc
    84721 ccataaccac caagacaccc gcaaatccag attcttccac aaatgccgca aatataaagt
    84781 cagattgaac ttctggaatg gagctgattt ttagtgtgcc aagtccgaca cccttccccc
    84841 agacgccacc ggaacgaatc gctctgagtg cgctatacat ctgataattc actgtttgcg
    84901 tgtgctgttc tggcatgaga aaaccaacca ggcgttgtac tctatactgc gaggaaaaga
    84961 tcataagagc accatagaga atcaaaaata atgctaggtt gctcatgaat agaaatggag
    85021 tacctgcgag atacagcatt gcaaatgcaa tggcgagtac tagaatagaa gtcgaaaaat
    85081 cctgttgaag aaagatcagg actgcggata tgatcacaag gacaatcgca ggcaaagcaa
    85141 ccagacccga ttcgacaagt cgctctttcc gtttttctag aaaagatgcg agatacaaca
    85201 ccattaccgg tttccagatt tccgaaggct gaagactgaa agaaccgatc cggatccaac
    85261 ggcgtgccct attgattgtc atccccagag ggctgaatat tggcaaagaa agaccaatta
    85321 cgcataggac gactgcaaca ggcaagaccg cacgcaggag cttcatagga ataaccgctg
    85381 ctacggccat acaaatgagc gatgccgcat agagaatagc ttgtttgatt attaccgagt
    85441 taggtccccc attgcgtatg gcaaaaccga ttgagccaga ccacagggtc attattccta
    85501 tcaatgagac tataattatt gcgcagacca tttgccaatc tattcgatgc gaatctatac
    85561 gcaagttctt gcttttgctc tcttctttca tctaccaaca acctcattga atcttcagcg
    85621 ttgacaatgc aatcaaggca aaaatacctc ccataatcca aaacctcacg acaacttttg
    85681 tctccttcaa accacttagc tcgaagtgat ggtgaagagg tgccatgagg aaaagccttt
    85741 tccccttggt cagcttgaag tagaccactt gcagcattac ggagccaatc tcgaccacaa
    85801 aaacaccacc gatgaccaaa agaagaattt cttttttaac catgagcgag agtaccgcta
    85861 ggactccgcc aagagagaga gaaccgacat ctcccatgaa tatctctgcc ggataagcat
    85921 taaaccatag gaatcctaca caggcaccta acaaagcaaa attgaacacc gtgagttcgc
    85981 tcgcctgctt gatatatgga agccccagat attgcgacca atctgctcgg ctagttacgt
    86041 atgtcaggac agaaaaagcc aggactgcaa agattacgag ccccacggca agaccatcta
    86101 gcccatcggt tatgttgacc gcattcgacc atgtggcaac ataaataatg gctattggca
    86161 accaatatac tccgagatct acgacatgga ccttgaaaaa tggaacatat agctttgtta
    86221 tagaaggacc tgttgtgcga taaatcgaat agacaattat agtcgagaca atgaattgta
    86281 ggattagctt ttgaacgaca gagagtccat ccgagctatg tttcttaatc ttgaggatgt
    86341 catccgcagc tccgattagg ccaaagccta taagcgccga aagtgcgatc caacaatata
    86401 cgcttgatat atctatccat aacacaacag atatcaagac cgacaatatt atcaaaatac
    86461 cacccatagt gggtgttcct gttttcgcca aatgactctg aggtccgtct aatcgcaccg
    86521 attgcccaaa cttgagcacc cttaaccgat cgataagcca cgggcccaag acatagctaa
    86581 tcaaaagagc agtgactgca gcgtaagcgg atcggaaagt cagatatctg aaaatattga
    86641 aaggggtaaa atattttact aaaggaagaa gaaactcacg aagcatgact tcctcctttg
    86701 ctcgattcag acaaatctat gagccctttt tcctggaaag cgtgcgagat cctttcaagg
    86761 gccatacttc gcgaaccctt tatgagcaca agatcacctt ctctaacata actggaaacc
    86821 gtatcaatca gattccccat gtctgtcgta tgcatcaaga cgcctctaaa agcagcttct
    86881 ttgagtgcgg cttcggctgc tttcatttct tctccaaaaa gaaaaacagc ctctggcttc
    86941 atttctgcca ccataaaacc cacatcgcga tgcgcattat cgctttcttt tccaagctcc
    87001 aacatggaac cgaggatcaa gactttgcga ttcgtcgatg atattccaga aaggaagtcg
    87061 atcgcggcag ccatgctgtt ggggtttgca ttgtaataat cctgaaatac agtgatcttg
    87121 cctttacaga tttccgagcg accaaaaaga ggctgcactt ttgaaagccc ctgaaccgct
    87181 aaaatcggat caagctccat aagtatcgct atcgataagg cagcacatgc atttaggaga
    87241 ttgtgtttgc cgggaagacg gaaatctaag gtctgacctt tccattcgat tttccaacca
    87301 tccagacctt tgttctcgat gcgcccaagt ccctcagtac tacgaagccc aaaataataa
    87361 acagaacctt tcacttttga agaaaggtag gttctgtagt cgtcgtcttc ccataacaag
    87421 gccttttgtt ggccatcgaa atatgagaat atcctgcttt tttcccttgc aatttcgtct
    87481 ctagaaccaa acatgccaac atgtgccgag cctatattgg ttatgagcgc aatttccggc
    87541 tcgtacattt tggctagctc gtccatctca cccttgcgat tgatgcccat ctcaaagatg
    87601 cctatcttgt gtcgaggttc aatgccaaaa atggcaagag caagcccaat atcagaattc
    87661 agatttccag gactcattgc gatgctctct tctgggaagc atgctgatag catagaagca
    87721 atgcattcct tggtagttgt cttaccgcta gatcctgtca ctccaattct gagtagattc
    87781 tgccttcttc gatattcccg agccgcattc tgaaggccag aaagaggaga atcaataagg
    87841 agaagacagg tttccgcaac aagaccgcta tctcgaattg aagcaatagc cgcatctttt
    87901 tcatctattt ccgcaagaat tgtccttgct ccatttttga ttgcctcgag aatatgatga
    87961 tgaccatctg ttctctcgcc cttgagagca acaaacatgc tgtttggctc tactagcctc
    88021 gaatcggtga caacggaaca tatcggagta ttaagctcgc ccttacaagt ggctccaatg
    88081 ataccagcaa gttcgcccgc gtagaacaaa acctgctggt cggaagcgat gctattgtct
    88141 tccattattg ctcacctccg aagccttctg ttcctgtctc tcggttacga tgatacgctg
    88201 ttccggagtc gcgactatca ttccttttct gattgcggct ctctcaatac gctccaggtt
    88261 ggcaagtttt gtgatatctg catccaattc tctgcccact tttagcagat ccagctcagc
    88321 ccgctctaat tctcttactt cggctctcaa agcagcatag cgcgttgttt gatacaccag
    88381 ggctataaac aaaaggcaga ttattattcc ggaaaaccaa aagcgccttg cattcatttc
    88441 tgttcctctc gagcatttgc tcgaatggct cgaaatttag cggatcgtga tgagggatta
    88501 tttcgtagct cttctaatgt tggagactgg ggctttttcc attcgagcga aaagtggcta
    88561 ttcttggctt cttccgcgaa tatgttcttg acaatgcgat cttctagtga atgaaaagtt
    88621 atcaccgcac agacggctcc ggggctgagc aaattcagag catcgataca agctctctca
    88681 agacgaccaa gctcatcgtt gaccaagatg cgaagcgctt gaaatgttcg ggttgccgga
    88741 tgtatgcgtc cgttgcgata tttgacaggc actgcaccag atatgatcga agccaactca
    88801 gatgatgtac ggatacgttc tttcttcctc ttttgaacta tcgcatgcgc aatttgtctg
    88861 gaaaagcgtt cttctccaaa tttgaaaatt gcatccgcta gttccatttc cgaaagtcgg
    88921 ttgaccagtt ctcctgcggt acactgtgaa tatggataaa gacgcatgtc caatggctca
    88981 tctgcataga aggaaaaacc tcttcctgag ctcctaagct gatacatcga gataccaaga
    89041 tcgaatagta cgagagaaaa tcttgattcg ccattctcat tgcaaagaca ggaagaaaag
    89101 tcaccgggaa tgcaataacc agctctctta tttcgaatgt cagaaagaac ctcatcatag
    89161 taaccattga ttattaaaag cctatctgca aacggcttaa gtcgctcggc agcacgattg
    89221 caagcatctg gatctgcgtc gatgccaatg caacgaagcc cttcatattt ttttaagagc
    89281 gcttcggcat gtccgcccgc tccaattgtg caatctacat agagggtcat gggctttatt
    89341 gtggaggcaa gattgcatag ctgttccagc attactggcg tatgtaagaa aaaatccatg
    89401 ccgctctctt tatcagagta gggtttttgc gttcgtatca tgcgcttacc ctgcatagac
    89461 aatccctcaa gaaagtgtaa gcccaccaag ctcttcggca gccataagga actccgattg
    89521 tttttctgcc atataagctt gatatctctg acagtcccat atttcgatat aacgagaaat
    89581 ggcaaggata acacactctt tcgataatcc agcccattct ctcaggcttt gaggaataaa
    89641 tattcgaccc agtttgtcca cctctacttc ttgggcagga gcaatgattc ttcgctgtat
    89701 taggcgcgaa cgggcttgaa aaagacttgt ggtggcaaga atccgctgag aaagaacttc
    89761 ccattcttca ggcacaaaga gccataagca agggtcaacc ccctgcgtga gtacgagcgt
    89821 cgacgataga ttagctcgca ggcgggcagg aagagaaaga cggcctttct catcaagagt
    89881 atttctgaat tcaccggtca aagatttcat tcccactatt tcctaccatt taccacaatt
    89941 taccacctat ttatcatttt aagcgctaat cttgatgtgt caacgaaact ttaggaaaaa
    90001 gaatcgacag aaaacaaaaa cctatctttt ttggctatac atatgtatag ggtcatgaga
    90061 tttggcagta tggaaatttt cagtcaagca gagtatcgac caacgaggca atcttgctgg
    90121 aatcgatgcc atctatgggc cggatatata ttctgattgt tctcaacgct tcagctactt
    90181 tttccactat attgggacaa aatagagttg aacgctgatc aaaaggttcg aaagaattgt
    90241 ccacttgaac catcaaatcg gtttctaact tgatccgctc aggaatatct ataactacat
    90301 cattttctgc tatatccgcc gcttgagcaa gcgcactttc cgcttccgtg cgctttacga
    90361 tatccaatag gtctctatgc agagggttgg attcatcgaa aacatgttca gccgcaagct
    90421 tgtatactcg tttctcttca gcttcccttg caagcctaaa aggagggaat ctaggactac
    90481 tcataagccg attgaaattc gtatcatcga taccatacag ttgcgaaggt ataaggacat
    90541 tagaagccaa tcccaggtac actgcctttc tgaccatggc tgttgcggct cttacaacgg
    90601 agtgccagta aacagcgcga tacatttgat actttgcaaa gatcagggct tcaatgctca
    90661 tttcaccttt caaatcgatg gcaagtctgt catcatgcac tatgagtcgt cgcattatgt
    90721 agtcagcatc ttgtatgcca tatggaacgc cacaatagaa tgcatcacgc gtcaagtaat
    90781 cgattttatc tggatctagt acacctgata aaaaagagca gaaaagaagc gtctctctgc
    90841 tcttattctc tgttcggtcg ggatctatga tagatgcggt ctgctcaggg tccgcgccac
    90901 attcaagaat gtgctctctt agaggacttg aaattatcat ttctccagca agagcttcgt
    90961 ggcgctcaag gggaagttct tttagagaat gcgcataggg ataatgcccg atatcatggc
    91021 aaagagatgc aaccaaaaaa gagcgaagcc cttccttgct gacaaagtct atttctccct
    91081 tttcaactaa agccaaggcc atacgccgcg ctatatgata tactccgagg ctatgagctt
    91141 tcctggtatg cgttgcgccg ggataaacca gtgctactgg tcccagctgt cggattcggt
    91201 ctaaaaccat gaaggctctg gattgaaata gcgcatggag cgatgaatcc atgtgcacat
    91261 ctccccatac aggatctctt accacaacat cgaagccatg caagagtgca ttcaagagcg
    91321 aaatttccga catgtccgaa tcagcctctt tttcaaagag ttttcaaatg gtctttatgt
    91381 ctatgaatcg acgattcaat tctaccagcc acctatcaga tcatgcaact cagttgactg
    91441 gtcgtaaaaa aatagtctga gcgccgactc cccataatgc taccaaaatg ctgactatta
    91501 tgcccgccag aatgacacct ccggcaatgg cgaaaaaagc cttcttatgc gaaagcccaa
    91561 gtatccatgc acccaaagtc cctgtccatg cacccgttgc tggcaggggt atagccacaa
    91621 agagcaagag accccaatag ccatattttt ccacttttgt gtgtacctta gctcgcgctc
    91681 tattcacaac tctgtcgaag aatcgcttat attgcggaat cttatataag agtctatgaa
    91741 ggctttccag gaataaaaaa gctataattg gtatacatat attagcggca atagatatga
    91801 tagctgcaat aggaatggaa aatcccttgt aaacggcgta tggaattgct cccctcaatt
    91861 ccgacactgg aagaatcgaa agaacaatcg tcctaatgag gtctgcattc atcacgggca
    91921 ctatagcgac ctcgcttcag tctgtctata gaaagagcaa atagatgaaa actagggcta
    91981 tgttacgcca tcttggaatg ccaaaaatcg cagaacgtca ctgtgcatag cctcgcttta
    92041 gctattcgat ccgatttctt tcaaaactcg ataaaaatct gcaggatatg gctctatcca
    92101 gataattcct ggcaaagcgc ggaaaaatgt catttgacgc tttgcgtatt gccttgtgtg
    92161 caattctatg gcactggata ttctttcgat cggatcctct tcgagttcga aaaattcgcg
    92221 atatccaatg gctcgaagac ccggatcctt agaactatag cccagactct taagtttctc
    92281 aatttcggaa gcaagacctt ccttcatcat ggcttcaact cgttcatgga ttcgtacatc
    92341 caattcctca cgacttcgcg ttattccaat aacaatgaat tttctacctg aacgaatggc
    92401 cttcttaggg gcaaatagac ttggcggttt tcctgaggtc ctgagaatct cgatggctct
    92461 tgtaagtcta tagatatctc gctcatgaat tcgagcggcg gcattgggat caacctccgc
    92521 aagctcttct cttaagactt gaggcccgaa ttcatccaga tctctaccaa cctgggcacg
    92581 aattgctcgg gagcttggcg gagaagtggg caagccacag atgaaattca tgaggtaata
    92641 gccagttcca ccactcaaaa taggcaaaat cccttgagct gaaagctgat tgcacaatgc
    92701 atcggctcgc tcgacaaact cgcctgcagt gtactgctca tcgggatttt ttatgtctat
    92761 aagatgatga ggaagtctct tgagtaaatc cttcgatggc ttagctgttc caatatccag
    92821 aaagcgatag gcttgcatag aatccgcgga gatgacttga gcagcattta gatcagcgtg
    92881 ccataggctt cgaagtcgtg gaatccataa atctgcgcct tcaccaaaaa tagtgtctaa
    92941 aagatgggtt ttcccagatg cagt
//
LOCUS       DAOMZL010000002        51209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_451_length_51209_cov_16.691813, whole genome shotgun sequence.
ACCESSION   DAOMZL010000002 DAOMZL010000000
VERSION     DAOMZL010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 51209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51209
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_451_length_51209_cov_16.691813"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1361)
                     /locus_tag="PLQ30_00450"
     CDS             complement(177..1361)
                     /locus_tag="PLQ30_00450"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPW00943.1"
                     /translation="MTYNKNKGSSHRHNPTEESLENLIKDALSKSLCTGIAAAVSMDG
                     KLFWSHCSGFTAREEPAAAQSIENHDQASKVVIAPIDAETRFDVASMTKPLSTSLLIL
                     KALQAGALDLKDPLGKYLPELNYKTGQIPILALLTHTSGLPAIPALHSLFPDSERLDR
                     NRAFEKLFSIEPEFAIGRHVVYSCTGYIFLGVLLERISGMLLGELFQKEIGTPLGLGQ
                     ASFAESIGTSGEPKPLGNAVATEYCPWRHRRIQGQVHDESSYCLGGHSGNAGLFLSLN
                     DAMAMGNFIMQEGAFNGNQILKPEIIQLLWREYTPGLEERRSLGFKLHDSATIDGPLW
                     PPQSFGHTGFVGTSIFFEPQRRIFSILLTNRVYFGREQTTDSIFAFRREFHSRVWRAF
                     AE"
     gene            1385..2569
                     /locus_tag="PLQ30_00455"
     CDS             1385..2569
                     /locus_tag="PLQ30_00455"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPW00944.1"
                     /translation="MSKSKQQRASVVLGLENFLDDTRRYNKRYGLATNSAAITSRGIP
                     SWKAFIEHGIIPACLFGPEHGFRGAAQDAVGLDDEVFRGIPAYSLYGKRLRPEPWMLE
                     SLDALVFDMQDVGCRYYTYLYTLAYIMEVCEKVGTPLIVLDRPNPIGGSKIEGSPISK
                     EAESFVGGYGLPPRYGMTIGEFALYLKGEYYPNCELEIVKMSGWNSRIAWEGTNLPWP
                     LPSPNLPTLSCAELYPGTCLVEGTWLSEGRGTSRPFEIIGAPFIDGEALREQLAAFEL
                     PGVVFISLFFSPSASKYKGELCEGVLVSLTDRQALRSLDTGITLVHTIHRMYPDKFRW
                     REDWDDKSLSFFDKLAGGTTLRCMITSGAPLEDCLEFAHRDESQFLRIRGKYLIYGAQ
                     KA"
     gene            complement(2638..3468)
                     /locus_tag="PLQ30_00460"
     CDS             complement(2638..3468)
                     /locus_tag="PLQ30_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPW00945.1"
                     /translation="MMFKFNGFQKALAWIFLLLLAFANLYPIGIMMLSSFKSTKEIFL
                     EPFSLPSVWRWQNYVSAWQRADFATYFKNSVLVTALSIVSIIIVSSMAAYVIARFDFK
                     FKRGIYLYMLAGLALPTRLAIIPIFLIMRNLHLLDKLSGLVVVYTASGIAFSVFLLVN
                     FFKNLPHDIEDSAKMDGAGPFRIYWQIDLPLLKPALVTVAMFNFIDVWNDFFFPLILL
                     SSKAKKTVPLGLQAFFGEYTVEWDVLFAALNISVLPILIVFLILSKQFIAGLTEGALK
                     "
     gene            complement(3465..4364)
                     /locus_tag="PLQ30_00465"
     CDS             complement(3465..4364)
                     /locus_tag="PLQ30_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPW00946.1"
                     /translation="MRKYSFLWMLLPAFILYSLFIAYPFFSSLGLSFYEWPGIGPKKF
                     IGLANYKNILTGFMSSEFYNALWHNVVFFIWSLILSVVPGLFFAFLLSANIKGTGWLK
                     VVFFFPNTLAIVVVGFLWGLLLNPQWGLINQLFRLVGLDFLAKPWLGDTKTALPTIIF
                     VTAWRGLGFYILIYLAAILGVDREMIEAARIDGASELQIAGRIILPHLYPIIATTSML
                     KFIWTFNIFDIVYAMEGTQGGPAGSTDVLGTLFYRIAFGGLGASQVGMGLGATVVTLI
                     FLIVLPVSIFYVFIVEKRVEKGQ"
     gene            complement(4469..5713)
                     /locus_tag="PLQ30_00470"
     CDS             complement(4469..5713)
                     /locus_tag="PLQ30_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPW00947.1"
                     /translation="MAKKLSIVLLLIAILSPMYAQQKTVSIWGWRAQDVDVWKGVETA
                     LKAKGEQISIRYEVFPPTEYDSKLLVSLQGGVGPDIMYTRRLPGARTQALLDNGYLTA
                     INGLVDLSNFDDVSLSFISTDNKVWGVPFANQIIGIFYNRAIFDKYKLKEPQTWDEMV
                     QIAETLKKNGVTPFFVTGKEAWILAMQNAMVGVSYPGDEWIGKLTVGKAKFTDPEYVN
                     MLKDLNALKKYYQKDFMANTSAEQDVAFAMEQAAMVFYGVWGNTNWLKMNPDLKFDYF
                     PIPPKDKKVPAKAYTYMDGAYGLNNTSKNKEAALKVLNYTGTKAYGELFSSTTGEITA
                     INGVTMPASKPILVKCYEKMTTIAAKNRYWVGSPFDAGTPSVYNILEKDMQSMYLDQM
                     TPEALAKRLQDGVSTWYPAFKK"
     gene            5898..6902
                     /gene="murQ"
                     /locus_tag="PLQ30_00475"
     CDS             5898..6902
                     /gene="murQ"
                     /locus_tag="PLQ30_00475"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HPW00948.1"
                     /translation="MDIDSVDEYLERATTESRNVRSEHIDALPVIEVLRIINEEDKGV
                     PFAVEKALPSIAALVEDVVKAFNSGGRLVYIGAGTSGRLGVLDASECPPTFGVPPDMV
                     VGLIAGGEAALRNSIEGAEDHPENGEKALRDINFSARDVLVGITASGAAPYVLGAMEY
                     ARNLGAVVAAISCNRQSRTFDIARHKILLEVGPEVITGSTRMKAGTAQKLVLNMITTS
                     SMIRIGKVYGNLMVDLMPVNRKLIGRSKRLIRQATGCSKDEAEAAFLASGGKPKIAIL
                     MTILGIGAEQAAELIEHGEGRLSEAIRIYSESKGDSKQENKGERNEARSARNNRRGNS
                     "
     gene            6859..7707
                     /locus_tag="PLQ30_00480"
     CDS             6859..7707
                     /locus_tag="PLQ30_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HPW00949.1"
                     /translation="MKQGALETIDAAIPNLAEAERKIAAFVQEAPQKALHLNISELAR
                     QAGTSPAAVVRFCRHIGVGKYSDFKLWLAKDVYQGWGEKFLPDLELESQTPGARAIRG
                     MTEAVRRTMNSLAQTLDPRAVEEAAERIKGASMTAIFGIGASGLVAEDFHQKLTRIGI
                     PSSYLFDTHAQITASCSLRQSDLAFIISYSGETNEMIEVAAQSKARGCFLISMTMTGQ
                     NRLRDYSDLILEIPTVERIYRSAAELSRISQLAAIDILFNVLISYDLDSAISSLERSM
                     QATHHF"
     gene            7835..8653
                     /locus_tag="PLQ30_00485"
     CDS             7835..8653
                     /locus_tag="PLQ30_00485"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPW00950.1"
                     /translation="MIRILLENDEFIVIDKPAGLAAQPGEGLRDDVITALERQFGYRL
                     FPVHRLDKDTAGCMMLAKDARAANKWSNRIASREITKRYQAWVAGLPNQEKGTIEDAL
                     HVKRGIQAACTLWTLRETWLFPLQEHSNRRADVQDGGHVHNNRIGVDRYEANSSIAAA
                     EANFLTLSLLDLELSTGRMHQIRRHLAGIGLPIICDDKYGDFSLNKALRKNGIRRLML
                     WAYELVLPLPSLESPNLPIFAAEPAHFISLRQTLEREGRILDKRNTEQSDEKTI"
     gene            8637..9077
                     /locus_tag="PLQ30_00490"
     CDS             8637..9077
                     /locus_tag="PLQ30_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW00951.1"
                     /translation="MRKPFEISTGAWWVVVDGRTIAVPSFHESWLASHPGIASGALHT
                     IDFVEKSGWLSVTLYSEGLIEVISRDILDNRQREALRQLLETNRSIIRKMVLFVPSID
                     GCFTAEDLLLDDWERLWKEIETFAAKEIRQNLSEEGMEVDTGQP"
     gene            complement(9091..9882)
                     /locus_tag="PLQ30_00495"
     CDS             complement(9091..9882)
                     /locus_tag="PLQ30_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HPW00952.1"
                     /translation="MHIGNVHIGTCGFSYEEWKTVFYPADLPSNEMLRYYSLVFSFVE
                     LDNTWYRMPNPAQLKRLALLTPSDFRISLKVHRSLTHEIDESWQTKANEFSLAVSALS
                     STGRLGCVLVQLPYRFSYTIENRKYLANLLAALSDFPLVVEFRNATWYQDRVFETLRK
                     LKVGLVTVDRPDLPGLPPESIIITSDICYYRLHGRNTDLWWSGNAKSRYEYCYSEQEL
                     KDRARLIRIMAKQSKTAFVAFNNHSNGYAPKNAAYLQSILAGQQL"
     gene            9944..10537
                     /locus_tag="PLQ30_00500"
     CDS             9944..10537
                     /locus_tag="PLQ30_00500"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HPW00953.1"
                     /translation="MVFSTLAKIVSSLVSLYMILCIVRIFFSWIPSLTDTKWGRLIAG
                     LTDPYLNLFRGFSLFRTPAIDFSPIVALAVLSVLNNLFMTLSYAVRITLGFILSLLLD
                     AAWSAISFLLGFFLVISLIRIIGFLARIAVLHPLWQILDGIINPLLFRINRIIYRGRT
                     VQYLQGLITGFIVVLVMRTLGGFLVRLLTSLLMSLPI"
     gene            complement(10584..12812)
                     /locus_tag="PLQ30_00505"
     CDS             complement(10584..12812)
                     /locus_tag="PLQ30_00505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW00954.1"
                     /translation="MAILLSAQKLAKARKTFNIYNFLNSFSFVFVSGSIITLFALKLG
                     AKNSIIGLLNALAYVTFFMLPLGKRLVKRSSIIKVFGWGWVIRYIALVPILFAPLFAS
                     KGHMGLAILILILGTAGFHISRGIALIGNNPVLGLLSTGGGDKPRSDQGRYLVNISVV
                     NSVAAILANIVLAFLLGQEASLWTYTIAIALGIITGLISCAILFKTPEPEAFSGKDEQ
                     SLWDVTKEALVQPDFRAFILTFMLLSFLSGMGRAFLPVYAKEVFAQGDDAIMVYSIVA
                     SLGSITMGLITRLVVDRLGPKPLFIIYSAIGFVSFLPIALIPGSATLMGAPSVIALFL
                     AIVHFLSSFGFAGEENAAQVYYFGLVPKEKMLDLAVIYNLAYGIGGSLGSFLGGGILE
                     VFANMELSDAGAFRLYYAAISVGLVIVIYSMRNLPHMGAKSVTQSISILLSPRELRAF
                     DLLTRLDQSASAHEEVRIIQKLSKSESNLTQKELVEYLYSPRFEVRSEALLALESIPE
                     LDHETIVALIREVKANVYTTAYVAARILGKQRSRIAIPALIESLDGPDYMLQSMAMIA
                     LARLQAFEAVGKIEQLMRSMENPRVRISGAYALELFGRIDSIPTLISCLKAEDRPASV
                     SDELVLSISGILGIDKEFYLLYSEFIDDNSYGIAQLIAYSKDLNLGDDRLNVWISAVD
                     ALFAPDSDTAPLARLLLASPRITNSILMLSDGLLDPGLTYPGFNFLCAYIALHIEDLK
                     DY"
     gene            complement(12881..13780)
                     /gene="mazG"
                     /locus_tag="PLQ30_00510"
     CDS             complement(12881..13780)
                     /gene="mazG"
                     /locus_tag="PLQ30_00510"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPW00955.1"
                     /translation="MDLDSLALRNIAGENADTLLADADRTAKAFRYFYVVVSRLRASD
                     GCPWDLEQTPSSIRGNIIEEAYELAEAITEDDSLHIKEETGDLYLLATMVGYMCQQEG
                     RFSVADSLCDEARKLIRRHPHIFGESEVLSPDQVVRQWNEIKEQVEGRRKKESILDEV
                     HRSLPPLEKAYKLQKKAAKVGFDWQHERDVWNKLEEELGELMQARAWKSEECPSDIAT
                     DSGSSKDALEEEFGDLLFTVINAARLYGIDPSIALQRTNGKFSRRFRHVEESMKQQHL
                     DMNQENMDMMDAFWNEAKRAEHS"
     gene            complement(13797..14606)
                     /locus_tag="PLQ30_00515"
     CDS             complement(13797..14606)
                     /locus_tag="PLQ30_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW00956.1"
                     /translation="MKNLEKQGFPAPKNESTPASEKSAKIESLTLLQLQKSSFLFFLI
                     TSMFVVFCCILMILMPEIAFLTYYSPEMLILIGLFSFGFLIVHLNRKLAWLGPVLIIA
                     LLPLTLTRYLTFMYSICAFIIALIELNRLGFFKHAGLFKACILISYYAWNFALVAIMH
                     KEFFLDIILSIAFLSLFFIYLIPIIQDRSQIGKVDLAYRIYPRDLNLTCREMDFLRDC
                     LNGATFKEIAYKHHVSESTVRNTFAHIYHKFNVVDRIDLMSKLAAFDIIDE"
     gene            complement(14680..15042)
                     /locus_tag="PLQ30_00520"
     CDS             complement(14680..15042)
                     /locus_tag="PLQ30_00520"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPW00957.1"
                     /translation="MNTIKSYKWTIASAMVVLLLLLMPFRIFQQTPQSLNDFDKFIHA
                     ILFGAMTAVFCAEHRALKKINPKFLLSLVIISAFAFLTEIGQLLTITRHFDLKDFGAD
                     AIGIAAALLVMRIATMLS"
     gene            complement(15169..18729)
                     /gene="nifJ"
                     /locus_tag="PLQ30_00525"
     CDS             complement(15169..18729)
                     /gene="nifJ"
                     /locus_tag="PLQ30_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPW00958.1"
                     /translation="MPNNKNMVMIDGNAAAAHVAHACSDVIAIYPITPSSPMGELADE
                     FSSMGRNNIWGTVPQVVEMQSEAGAAGAVHGALTTGALSTTFTASQGLLLMIPNMFKI
                     AGEAIPTVFHIAARAIAAHALSIFGDHQDVMATRQTGWALLASNNVQEVMDLALIAHA
                     ATLKARVPFLHFFDGFRTSHEVSKIEELSYDIIRAMIPEEKVREHRARGLSPEHPVIR
                     GTSQNPDVYFQGRETINKLYEAAPAIVEETMSQFAEMTGRAYKLFDYSGAPDAERVII
                     LMGSGADTAEETAEYLAKQGEKVGVTKVHLFRPFSVEHFIKAIPATTKSIAVLDRTKE
                     SGAIGEPLYEDVRTAIGEAMGNHTAPFKNWPIVVGGRYGLGSYEFTPAMVKAVFDNLK
                     LEHPKNHFTIGIDDDVSFTSLPYDEKFHLPGDGIIQCLFYGLGSDGTVGANKNSIKII
                     GDETENCAQGYFVYDSRKAGTYTISHLRFGPKQIKKPYLITKADFLACHKFSFIERID
                     ILSNIKEGGTFLLNSPYSADEVWDNLPIEVQKEIIDKKLSFYVIDAMSIADKAGMGTR
                     INTIMQTAFFKISGVLPEEQAVELIKKYTEKTYLRKGADVVQKNLDAIDLALNATHKV
                     KIGQADSIKHMLPPVPQTAPKFVRETLGEIIAQRGEKMPVSKLPLDGTFPTGTTQYEK
                     RNIAEKIPVWEPSLCTQCGNCSLVCPHGVIRMKAYDPAILENAPSTFKSTDAKGKEVA
                     GLKVTVQVAPEDCTGCGACINICPAIDRANPGRKAINFGPQIELRDTEKQNWDFFLSI
                     PETDSKFLNLSIPKGIAMKQPLFEFSGACAGCGETPYIKLVSQLFGDRAIIANATGCS
                     SIYGGNLPTTPYTKRADGRGPAWSNSLFEDAAEFGYGMRLTSDKQNEYARELLASAKG
                     KGIPEELVDRLLSNTQDSDEQIEAQRADVAALRESLAGKSESWAKELDNVAEALVRRS
                     VWIFGGDGWAYDIGFGGLDHVIASGKNINLLVLDTEVYSNTGGQASKATPIGAIARFA
                     TAGKETSKKDLGMIAVSYGYVYVAQIALGANMVQAIKAIREAESYNGPSIVIAYSHCI
                     NHGIDMMKGMDQQKMAVQSGMWPLYRYDPRLKAQGKNPFQLDSREPDTSKLERYMYAE
                     VRFKSLVQANPERAKVLLEKQRELIGRRYNEYRYLADRPF"
     gene            19639..21072
                     /locus_tag="PLQ30_00530"
     CDS             19639..21072
                     /locus_tag="PLQ30_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPW00959.1"
                     /translation="MVIQDKEQLEKLFESQAERRWKLALTGPEERIVKLKKLRSAILS
                     RSDELYAALNSDFGKPAFEAWLTEVFPCIEEIDVAVRHLKVWMKGRRAKGTLILPFSS
                     TRVRYEPKGRVLIMAPWNYPFQLLIAPLVSAVAAGNCVIAKPSNKTPHTAAFIADLIA
                     GVFDSQEVAIVEGSGSTLGDFLLELTFDHIFFTGSPAVGVKVGQAAARMHAGLTLELG
                     GKSPAILLRGANLKQASKRIAWGKFLNAGQTCIAPDYLFCPMELVGEFLAEMRLAIES
                     FYGKSEEERQASPDYPRIVDKAACARLERIVSEAISKGAKLEFGGRFDVENRYVSPTL
                     LTGVQIDMAIMAEEIFGPILPVLAYDSLDEAIEFIRSRPKPLALYILGKDKKSAERLL
                     AETTSGSVGINDLIVQIENLDAPFGGVGMSGTGSYHGFYGFKTFSHERTVLYQGPISA
                     VEKFYPPYNTSTQKRLLGALKKIKNIS"
     gene            complement(21120..21986)
                     /locus_tag="PLQ30_00535"
     CDS             complement(21120..21986)
                     /locus_tag="PLQ30_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPW00960.1"
                     /translation="MDDTIFPDSIDRFIQDALTPEFWSKAFTILITAILILVFFRILR
                     IILGRGMKKTIPEQKAQLILKALRYTGWAIAVVSVLQSVGVNLSALLGAAGIAGIAIG
                     FAAQTSVSNLISGIFLVFEKPFQISDTIQIDEISGIVMSIDLLSVKLQTFDNRFVRIP
                     NESIIKTNVINLTRFPIRRLDIYVNVSYTTDLRKATDVLRDIAYKNIYVLDNPSPLIL
                     IDKFDIPGVGILLGVWFEKSNLVEIKNTILMDIQERFAEEGIEIPYPKRDVYIKGIAE
                     RNLIEIDRKENT"
     gene            complement(22000..22845)
                     /locus_tag="PLQ30_00540"
     CDS             complement(22000..22845)
                     /locus_tag="PLQ30_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW00961.1"
                     /translation="MWNFFVPKESQWYCWKLDGASLWMRKNGEEWRFAASSIPFKQIQ
                     NDSAGPLFVDAPSELSIAFAVAPGKKAALRPHPSTAPYLVSARNDVKIYPGTEAQFDI
                     ALPPIFRIELENGSSLFESLPFVTPYTWFGDKVSGDLCLSLPIELDPQCKNEQGYFER
                     IDSNNPISLDPIIQESLQRAAQYLSCRSLIHCKIVVKNRSKEPLNVKRLAIFTSLMNV
                     YQKQDNLVSDTVIITAAADDSLQTNIDDSAYKNLPKIHAAPKTGLNELLVMKGVSFLR
                     SIAGL"
     gene            complement(22967..24133)
                     /locus_tag="PLQ30_00545"
     CDS             complement(22967..24133)
                     /locus_tag="PLQ30_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HPW00962.1"
                     /translation="MKGKRLLMIPGPIEFTLEVLAEMAKPTLSHTDQQFIEEFGQALE
                     KMRKVWLAPNGQPFAIAGSGTLAMEIAAANITEPGDNVLIVHTGYFSERMADIFRLHG
                     ANVDIVQSAVGDIPEIDEVRRRLKSKNYKILSITHVDTSTGVRTDVKALSAAAQETNT
                     LVVVDGVCAVAGEELRMEEWGVDIAFTASQKAIGVPPGLALLVTSKKAMESFRSRKSP
                     VGAYYCDWQYWLPVMEAYEGRKSAYFGTPAVNLVRALNISLGQILDEGMEARFERHAR
                     NAAAFRAGLSAIGLSFVPVRKEIMANTLSAVYYPEGTDASILPLIAKNGAVLAGGLHP
                     AIKTRYFRIGHMGMSDASEILAVLGSIEQGLATAGYSFEPGASLKAAQSALIQP"
     gene            complement(24299..25087)
                     /locus_tag="PLQ30_00550"
     CDS             complement(24299..25087)
                     /locus_tag="PLQ30_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPW00963.1"
                     /translation="MDLVPEKKPNPAIKQVIGVISGKGGVGKSMVASLLAQSFAARGL
                     KVGMLDADITGPSIPRLLGIDCFRGESDGENLYPVQNEEGIKVLSINLFNENEDDPVI
                     WRGPLLAKVIDQFWSDTIWGDLDYLIIDFPPGTSDVALTAFQTIPFSGLVVVATPQDY
                     VTMIVRKSVNMASMLKTPVLGVVENMRTMVCPHCGAEIALFDDGSQNGAKRMELPLLA
                     SLPWRKELAQARSLRWSALSQEIKRDADRLANEVEFALTAFSPK"
     gene            complement(25217..26284)
                     /locus_tag="PLQ30_00555"
     CDS             complement(25217..26284)
                     /locus_tag="PLQ30_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPW00964.1"
                     /translation="MSHYKNLVFKGGGVRGIAYIGALQYLYERGIMPHIRRVAGTSAG
                     AITALGVAMNPTDFMGFRTLADSLDYRKVPSEQDENTEKLTSKRLIAIIEHYRELAIF
                     KNLQCSIRLLQEKGWYSSEYLYTWLRSIIAEYFTVAKEIYTFADFANTQIHRDGRPFL
                     DLYITGTDVSNRTARLFSLETTPDMEVALAVRISMSIPLFFEAIQYQYPGTEEPQFYV
                     DGGVMWNYPINLFDDHKYCRKLNDGANAETLGLFLFSSSEKTHYPPIKSMLDYMRSLF
                     ESVSTVQEQLAIRTEKNYSRTIYIDDCGIEATDFDIQPGDERHRMLIDSGFRATREFF
                     ESKTEWSQFLAFLRERFGWKE"
     gene            26578..27378
                     /locus_tag="PLQ30_00560"
     CDS             26578..27378
                     /locus_tag="PLQ30_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPW00965.1"
                     /translation="MNIENHVMQTFFATLFTWGMTALGAAMVFFFKEINKKVLDSMLG
                     FAAGVMIAASFWSLLSPAIELSESMGLIPWLPAVIGFLLGGAFLLGVDHLLPHLHIGA
                     DMSEAEGLHTSWQRSVLLVLAITLHNIPEGLAVGVGFGAIAADIPSASLSGAIALAIG
                     IGLQNFPEGAAVSIPLRREGLSRRKSFVYGQASGLVEPIAGILGAVLVLAMRPILPYA
                     LSFAAGAMIYVVAEEVLPEASRDSNGHLATIGTMCGFALMMLLDVALG"
     gene            complement(27597..29228)
                     /gene="pruA"
                     /locus_tag="PLQ30_00565"
     CDS             complement(27597..29228)
                     /gene="pruA"
                     /locus_tag="PLQ30_00565"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HPW00966.1"
                     /translation="MNNGIFHIPRPINEPVFNYAPGSLERATLKKELDHLRTNQIEIP
                     LIIGGRELRTGKTATIRRPDAHGEVLGVYHLAGETEVKMAMEAALAAQEGWAAMPWEE
                     RAAVFLKVAELISGKYRALMDAATMLCQSKTAYQAEIDAVCEAIDFLRFNPWFMEQIY
                     SQQPDNVKGEWNRSAYRPLEGFVYAVTPFNFTSIAANLPTSPAMMGNTVVWKPASNAV
                     YSNYMLMRLYMEAGLPPGVINFVPGSGSLISNVVLASPELAGFHFTGSTEVFRSLWKQ
                     ISSNLEYYRSYPRIVGETGGKDFIFIDPSAELEPTVASTLRGAYEYQGQKCSAASRLY
                     IPESRASEFIEALVAQISTIPMGEITDFRNFMGAVIDETAFENIHGYIERAKAQGAKV
                     LAGSNCNKSHGWFIEPTLILTEDPRSETMVNEIFGPVLTAYVYDDRDMEAAYRLIDST
                     SPYGLTGAIMATDRERINRALLAFQNSAGNLYINDKPTGAVVGRQPFGGARASGTNDK
                     AGSIINLLRWVSALAIKENFSPPADWRYPFMKGDD"
     gene            29374..30618
                     /locus_tag="PLQ30_00570"
     CDS             29374..30618
                     /locus_tag="PLQ30_00570"
                     /inference="COORDINATES: protein motif:HMM:NF019244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HPW00967.1"
                     /translation="MLIVLVFIFLAGIIEGGTQAILGFLRLQITPARLVSDFIAIAGV
                     GSTLLNVSTVGFLGYGFLAINKLRLTGASLAALFTMMGFAFFGKTPFNCLPIMLGVSF
                     SALLVRKKPRDYALIAIFGTAMGPLITFIAFELGVKSFLALPASFAIGLGVGLILPPI
                     AIAMLRLHQGYNLYNMGLTAGFLGLFAASLSHATGADILPIEIWGTAHSPILVALLPI
                     VLLIALFCIVKEDPKNIVALFRHAYLDFRKILGMSGRLPSDFSDFVSTKGAFLNMIVL
                     GLFFWLFMLIIEAPFNGPVLGGLFTILGFSFFGKHIKNVFPIVLGIVVAIFVFDKDLY
                     EPGPLLAILFGTALAPLSGEFGPLLGFVAGFLHLVIVDRTCFWHGGMALYNNGFAAGL
                     TATLIVSVIDWYRSSKVANKVE"
     gene            30671..31054
                     /locus_tag="PLQ30_00575"
     CDS             30671..31054
                     /locus_tag="PLQ30_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW00968.1"
                     /translation="MKRHDLYVQLISELANYLLRNNPKKMVISLHLENDGFHIVAIDD
                     RYHSDEEIAEIEQGLKMHQRPELASYYGTMLGRDLLGDARLELIGYHVKGAKVEKIPE
                     GGIMINLWVGSDDFNPAMFTIDEKA"
     gene            31087..32544
                     /gene="sbcB"
                     /locus_tag="PLQ30_00580"
     CDS             31087..32544
                     /gene="sbcB"
                     /locus_tag="PLQ30_00580"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HPW00969.1"
                     /translation="MAVSILWYDLETFGRNPRHDRIAQCAFIRTNIALEEVAEPVVMY
                     CRLPLDYLPDPEACYIHGITPQEAEEKGLSEYEFSQRIQREMSVPGSTVTGYNSIQFD
                     DEFIRNLFYRNLMDPYSREYKNNNSRWDVINLMRAVHDLKYEGFEWPINGEGNPLFKL
                     VDLAKAQNVAHENAHDALYDVRATIGLARKVLETYPKLFEWYYRHRRRENLARLVDLS
                     RRENMLVHTSQRYTRQGGCSTVITPLGLLEDDRHALVTYDLRFDPKPFLYMSEDELRD
                     LLFSERQEDEKIRPPLNMVRLNSCPYLAPLSVLNRQARARLGIDLVECRENLRILEAN
                     PDLKARFIAAYRFERKSVDELDPEHALYAGGFYSDEDRRELERFHRVLMNSGPQAAKN
                     EFLRIRFQDPRIPTLMGRLLARNFPETLTADQHLSWKKHVYGWLQLPQEKGATALSDY
                     VRLERELQEMSPDDPRRPIAMALIKWKDKLCSQAI"
     gene            32738..33229
                     /locus_tag="PLQ30_00585"
     CDS             32738..33229
                     /locus_tag="PLQ30_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW00970.1"
                     /translation="MKYRWFFAIFLALAGLTILSAQEKSLESTMLIRVEKAAVRAEPS
                     FVSPALSFAAYKTPVAIIRIENDWIFGFVRGLNRPGYIHMSALSPVKVSLNPDDVSDP
                     PPLQETEIVLAGKGFSSSLELSLRESSAFNFEAVDAMEKNSYSYAECLAFIHGIDILE
                     GEP"
     gene            33231..34316
                     /locus_tag="PLQ30_00590"
     CDS             33231..34316
                     /locus_tag="PLQ30_00590"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPW00971.1"
                     /translation="MRKIVLQSRVLSVILALILILPFFASCTITPESLQFFLDQALLQ
                     GMITPEQSRLILEAVRSFQIESEPFTYEEEYVIGRVVAAAVLSQYQVYDQPELTAYIN
                     KIGQGLAFYSARPCLPEGYHILVLDSEEAHAFAAPGGFVLITRGLLKLTGNEDMLAAL
                     LAHEISHTALGHGLLALTSYRMGDLAKYYVMLSTESAQSYNKEAMELFLAAVHDFVSL
                     ISVQGYSQVMEFEADAEAMRMLYEAEYRPSALGDFVVTLENSKDERFQQYSMQHPLAE
                     DRLSRIGSLAEEYSQRAELEHRKECVQRLDSLLQNEDESWHNSNLACEQGFLDSGYEL
                     AKENLIISPVSLELLRQERFEAIRPLF"
     gene            34338..36446
                     /locus_tag="PLQ30_00595"
     CDS             34338..36446
                     /locus_tag="PLQ30_00595"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HPW00972.1"
                     /translation="MKYMTEAGKPDHSTLRSNHALNKVKRFLYALVAGVFGILLFQWT
                     GISNKAGMALYDREMRLLVAAEGKKEDNTSSQTLRKNSGSSDSASLSPSRKKAPIALV
                     MIDQSSLDWVQNELGLGWPWPRELYGLIAYSLRNADVQAYDILFTEPSTYGPEDDSRC
                     AEAMRSAGNVVLATLTDRKPVLAVSNALYGHVVARVDSDGICRQYQAALDTDAGKIPS
                     LGLAAISALNRKNSIADEKSISLSEAFEPLEPLAPFEAFEQTVFLKFEDSSRFERYSA
                     AQVLAGSPGTGGSDFAGLNQELNFEGKIVLIGITAPGLMDRQATPINPAQPGVEIHAT
                     FISNALSSSFIELSPIGVEVCITIIALVLAALLPTMRTRIWTIVGFVAAIIIPIGLSF
                     AFFRNLVFYNAVPAIAAGLFAFIAAIVLGYQAEGRQRAYLRKAFAQYLSPEVISELIE
                     QPKVLRLGGESKVITTLFSDLAGFTAISEKLDPERLTQFMNEYLGIISEEILAQGGTL
                     DKYVGDAVVAFWNAPLDIEDHALRACMAACHIQEKLREIGPELEKRFGISPRTRIGIA
                     TGSAVVGNLGSTHRFAYTAVGDSVNIASRLENLNKVLGTAILTIRETVAAACAGSSRA
                     SSPSSLVPAAQDLDSELAPEIRLPQLEGKTLLFRRLGPAIVEGKLQAIELWELRATEK
                     DNCAQGSLIEPWQEARHFSK"
     gene            complement(36451..37266)
                     /locus_tag="PLQ30_00600"
     CDS             complement(36451..37266)
                     /locus_tag="PLQ30_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPW00973.1"
                     /translation="MADRIAAESTANPFAFEPGESKLVMFHPLAKLVFLIAATSFAMH
                     APVGFLLALFVIGILPLLKLPRAAAGPFYSIVVLMLFATIVRGIFPGDGRIFDLATLP
                     GSAVYGLRLFSIYIYSRLFYATTRVSEIGDWMTAGLRSLRKIACRREAKDNPTSRLNQ
                     ESTYYRPVSMDISLDPGMFVSLILLFLPRIFNMYRRVAEAGEMRGISISKKNFRRAFL
                     MLGQLISMGLLQSWRISMAMELRGYSPYRSIRLSRFKKQDGILLGLSAIMFLL"
     gene            complement(37251..37994)
                     /locus_tag="PLQ30_00605"
     CDS             complement(37251..37994)
                     /locus_tag="PLQ30_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW00974.1"
                     /translation="MAELFRAVSLSHIFPDRGIGIDSISLSIEEGEFLLVAGRNGAGK
                     SLLMRHFVGLSKPSAGEILYRGIPIALQIPLIRREVGYVFQDTEAQVFGLTVGEDLAF
                     GPANLGIKGSKLDAVIKKALAQAHLEGMENRSPGSLSGGEKRRLAIAGVLAMQPRCII
                     LDEPFANLDYPSVQEILEVFGTLHSDGKTLIVLTHEIEKVFSLASRLLILDRGKIVFD
                     GLPGEATIGLFLRSGLVCPYQEGYPWLIE"
     gene            complement(37987..38547)
                     /locus_tag="PLQ30_00610"
     CDS             complement(37987..38547)
                     /locus_tag="PLQ30_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HPW00975.1"
                     /translation="MEGNNAKLSQAIMACLFAALISVGAYAAFPIPGTPVPIVLQNMF
                     IMLAGFISGPWWGLVSVVFYLILGSVGLPVFSGGTGGFAKLAGPTGGYLVGYIPAVIL
                     FGLISKLGKGKWIFNILAGISGMIIVYAAGIVWLKSVLDIGWERAIATGVIPFLPGDI
                     IKIILASVLTPPILKAMSHIDQQTNG"
     gene            38957..40213
                     /locus_tag="PLQ30_00615"
     CDS             38957..40213
                     /locus_tag="PLQ30_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW00976.1"
                     /translation="MKSFASLDAAGTSIGSILERFQASLADKGLSFNEFLSDAYVQKW
                     KGEQLAHALKVRTALVLGLQEFLVSRGILNIDRVSLSPVTDPLCHDVEHVPVILYQDT
                     PYRTTHSMIYSKMLACLNPYIEGIYIDSSNIRLELPSSTSRHKYLIDFSQMDIEFKRA
                     KRLSEDDYFNRPKETTQVLTKELDRALDFFEDLIIYGVKKILVLAAESLGALGVKLTV
                     PSKPFPRFYSDEIDMQGDCSLEGSLEERLGHKAKTQFFWVLGLLRENYDLVYPYLSRD
                     GRRPEKKDISPRQVFNYDLCAAPLYRDESLGAAYEILSGGLREWIYESIVSRLLDNAV
                     LRESPRFDRNGNIENMAALDGYGPFLSIAHMRDAQGQGIFPQVCGGGLGIERTLFALL
                     RGPFIKDIDEVTCFGKNPDKTLPFLF"
     gene            complement(40301..41587)
                     /locus_tag="PLQ30_00620"
     CDS             complement(40301..41587)
                     /locus_tag="PLQ30_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW00977.1"
                     /translation="MDSYDVVIVGTGPAGLGAAFKLLESRPSFNILLIDKQQVSSGGL
                     RNDCKMNFTWPIGFPLECWDEATGKHYLEHTEGFLKPHIMEKRNIEIYRKRAEKIGVQ
                     LLDVRQSHLGTDGGFKLIKDLTARLRGLGATFSFKEEMLGVEPGSRVIITDKRELKYG
                     SLVVAPGRGGFAFLQRLMEELGIKFRDNVVDIGVRIETTEEHYPIVRDYYDPKFFFPK
                     KTRTFCTNSRAAYVVQERYGDEKQGYWYSINGHAWSSERPANGLVNFAILKTITLTQP
                     LASGQDYAQMLGHLAVLLGGGRPIMQRIGDFRLGKRSFANSFTGDLYNFNPSLPSCTP
                     GDISLCIPAKTMRAIWNAMKLLDTIVPGVLHPSTIMYYPEIKLYANRPVFIDENFQVL
                     PGIYFIGDGAGTSRGITSAWASGMRAANGIISASNL"
     gene            41833..42984
                     /locus_tag="PLQ30_00625"
     CDS             41833..42984
                     /locus_tag="PLQ30_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW00978.1"
                     /translation="MKRIVRIGLLSLGIAALIVACGPGAPKTVKFGVFEPLTGANAGG
                     GALEVEGIKLANELYPTVMVGDKEYKIELVIADNKSDKVEAANAAQRLVDKDKVNVVL
                     GSWGSSLSMAAGPIVKNAKVSAIGLSCTNPLVTKGNEYYFRVCFIDPFQGTVMANYAY
                     KTLGAKKAVIIREVSNDYSVGLAKFFADAFIQLTGDEDAILAELNYNTGDTDFSAQLT
                     EIKKHKPDVIFAPGNYTESALIIKQGKELGIKTPFLGGDTWETPEFIDVGKEAVEDVV
                     FSTFFATETPITETSKIFLDEYRKKYNKEPAAVTALGFDGYLVARDALQRAGSLDKEK
                     VRAAIAATKDFPGATGMITLNEDGDAVKSAVIKTIKDGKFTYMATVEPY"
     gene            43133..44026
                     /locus_tag="PLQ30_00630"
     CDS             43133..44026
                     /locus_tag="PLQ30_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPW00979.1"
                     /translation="MTLSLLFQHIVNALSLGSLYALLAIGYTMVYGILRLINFAHGDI
                     FMVGAYVAFYGITLLSLPWWAALLLSIIFTAFFGIGLERVAYKPLRDSPRISIMISAI
                     GASFLLESLAVLVFGGRPKGMPVPDVLSKVIYFGSVSMITISLIIPLFTFALLILLLW
                     IVNKTKIGMAMRAVSTDIEAARLMAVDVNKVVSVTFGIGSLLAAFGGIMWSFKYPQLN
                     PLMGVMPGLKCFIAAVIGGIGNITGAVLGGFLLGIIEIMMVALLPTLSGYRDAFAFIL
                     LIIVLLAKPTGLLGKRQAEKV"
     gene            44029..45147
                     /locus_tag="PLQ30_00635"
     CDS             44029..45147
                     /locus_tag="PLQ30_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPW00980.1"
                     /translation="MSKKSSTRHILKDRHIENGAISKNMLYTIIFLGACALFIFLADR
                     LFDAFSLRVFNLCAIYIVLALSLNLLNGFTGLFSLGHAGFMAVGAYVSALLTMPAPLK
                     EMNYFLLPIVPWLRHVTIPFLPALIIAGLVSACVGFLIGAPVLRLRDDYLAIATLGFG
                     EIIRVIITNAQSITNGAQGLKGLPRFSTTFAVWAAAGLVVLFMVLLMRSSYGRAMKAV
                     RDDELAAEAMGINVFRVKVTSFTVSSFLAGIGGALLGHMITTIDPKMFTFTLTYNILL
                     IVVLGGVGSISGSVISAIIVTVLMEALRFLDEPINLIFFEIPAMPGLRMVVFSILLMV
                     MIIYRQRGLMGNKEFSWDMFQSAFVKKSKKGGSHARTP"
     gene            45131..45907
                     /locus_tag="PLQ30_00640"
     CDS             45131..45907
                     /locus_tag="PLQ30_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW00981.1"
                     /translation="MPELPELMLSTSHLTMRFGGLVAVSDFNLEIRKNEIVGLIGPNG
                     AGKTTAFNVITGMLVPSEGKVLFKGKDITGLKPHSITALGIARTFQNIRLFKEMSVLE
                     NVLVGCDLSINTGILAATLHLPGYRTRESEARRFSVELLEQVGLAHLANEKGTSLPYG
                     QQRRLEIARALATKPKILLLDEPAAGMNPQESFELMDFIRKIRDEFDLTIFLIEHHMQ
                     VVMGVCERMYVLDYGITIAQGNPSSIQKNPKVIEAYLGVD"
     gene            45907..46617
                     /locus_tag="PLQ30_00645"
     CDS             45907..46617
                     /locus_tag="PLQ30_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW00982.1"
                     /translation="MLEIKNLSVFYGGIHALQGIDLDVPDRKIVTLIGANGAGKSTTL
                     RAIAGLIKPSSGEVILQGSVISGRPTLEIVQKGLALVPEGRRIFSNLSVEENLLLGAY
                     SRTDKKAVLADLEHVYELFPRLKERRGQKGGTLSGGEQQMLAVGRGLMSRPKLLMMDE
                     PSLGLAPLISKMIFEIIKQINSEGTTVLLVEQNAKAALEVADYGYVLETGRISLFGTG
                     HELLQDDRVRKAYLGEAQ"
     gene            complement(46655..48145)
                     /locus_tag="PLQ30_00650"
     CDS             complement(46655..48145)
                     /locus_tag="PLQ30_00650"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPW00983.1"
                     /translation="MKFLRRIIPWILVATVILAALYIPDLGPENYRDFDSLFRNERVR
                     QGIAGYEVVIIKGGEIVFNKAYGFDGQRNPLDISTPLYMGPASEIFTGTLLAQLVNEK
                     KITLDVPITHFIPELGSLKARTASDDSLDLPLTLRLLAAHRVAFPEKDLPEFDPETMG
                     LEAGLPDADLFLSSHFPTEKYTRSRLSYRIVGTILEKASGMKYSELLESKIAVPLDMR
                     STTAEPSSMLSIAVGAGSFFGLTFPYVEKVPYDAAPSDGIVTTSEDLAKFLRFVVSPK
                     RGQRIPGLSSAQTPMLYQPLYKDGDTGFGWRIIENKEGRSIFQGGSIRGYASRIVIYP
                     ERNAAIAILCPQDGVLISNFLLPKLVSCAEQILFEGESGRPFPTQRAQLVLGFIFIIY
                     LLNIILQTLVSYSWARNLLKYRETGISQLYARFVILRTIIGLVLRILIVLLAPLAIGA
                     LVGWQVTYQEIIAFEPGVSTMLMTALLFGALRNIARLVMVLRLRHF"
     gene            complement(48275..50428)
                     /locus_tag="PLQ30_00655"
     CDS             complement(48275..50428)
                     /locus_tag="PLQ30_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPW00984.1"
                     /translation="MDEHSSNFSLIDHFGSRCFSNDVMRERLPRSAFEAILEIQAGKR
                     ELTLDIAEIVAAAMKEWASDLGATHYTHWFHPLSGLTAEKHESFVSPQPNGSILMEFS
                     GKDLVKGEPDASSFASGGLRATFEARGYTAWDVTSPAFLKRNAERYILCIPTAFVSYT
                     GLALDKKVPLLRSMEALNRAGLRLLRLIGDSKTERIIAYAGPEQEYFLVNAELYAKRP
                     DLKLCGRTVLGTMAAKGQELEDHYYGAIDEKAAAFMNELNSELWAMGISAKTQHNEVA
                     PNQFEIACIYSQENIAADANQLVMETIRKTAEHHGMAALLHEKPFAGINGSGKHINWS
                     IGTDEGLNLLDPAIVAGISSEEEAGFKFIRFALFSLAVIEAVDRRAGLLRSSTATAGN
                     DRRLGGHEAPPAILSIYLGKPLYDLFNSLQNQNNAQRITNAFLELGIRSLPKLPRDFS
                     DRNRTSPFAFTGNKFEFRTVGSSQSAATSLTMINAAVAEALNRISEEIEQELTKGAHL
                     LDAARLVAQRSWQEHRRVVFNGNGYSIDWMHEAEKRGLPVFRSAVDAIQELSTPDNVK
                     MLEDLCILLPEEIEARQHIYLERYSKQIKIEAAMMIDLVRRYIIPAASEAASNYGSIC
                     SSIAAIGAPAIVQEALAKRIAQLVGKSAEGLDRLERALSDASCTEDELERAKSYRDMV
                     VPGMDELREFCDELERLVPKYLWPLPTYSDLLFTL"
     gene            complement(50779..>51209)
                     /locus_tag="PLQ30_00660"
     CDS             complement(50779..>51209)
                     /locus_tag="PLQ30_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW00985.1"
                     /translation="ERGADILLLADHFVSQFNQENSKKIQRISTPALDLLMIYHWPGN
                     VRELENCILRAAILSTDGVIHAYHLPPSLQSAASTGTEPKTGLDAEIFRVEKELIIEA
                     LKLENGNVSAAARRLVITERRLRYAIQRHRIDPRRFKTKL"
BASE COUNT        13383 a        11568 c        12452 g        13806 t
ORIGIN      
        1 aagaaaaggc agcagttctg ccaatgtaat tcttttgggg cttcttgcaa gcatgggcaa
       61 agccctaaaa tatattttca ttatataggt tgtttttcag atgtcaataa agaaggacat
      121 ctaatattaa aaaaatattt cacaaaatgc ctattgcgaa tttctagata tcttggttat
      181 tcggcaaaag cgcgccatac tctcgaatga aactctctcc tgaacgcgaa gatagaatct
      241 gtggtttgtt ctctcccaaa atagactcta ttggttaaga gaatcgagaa aatccttcgc
      301 tgtggctcga agaaaatgct tgtaccgaca aagcctgtat gtccaaaaga ttgagggggc
      361 caaagtgggc catcaatggt cgcagaatcg tgaagcttaa agccaagact ccgacgctct
      421 tctaaaccag gggtatattc tcgccataat agttggatta tctcgggctt caaaatctgg
      481 tttccattga aagctccttc ctgcatgatg aaatttccca tagccattgc atcatttaga
      541 gacaggaaaa gcccggcatt ccccgaatga ccccccaggc aataggaact ctcatcgtga
      601 acctggcctt gtattcttct atggcgccat gggcaatatt ctgtcgcaac agcgttgccg
      661 aggggtttgg gctcacctga tgtgcctatg ctctctgcaa aagaggcttg tccaagccct
      721 agcggagtcc caatttcttt ttgaaaaagc tcgccaagaa gcattccgga gatgcgctcc
      781 agtagcacac ccaagaatat atagccagta catgaataga ctacatgcct gccaatggca
      841 aattcgggtt cgatagaaaa tagcttttcg aaagccctat ttcgatcgag cctctctgaa
      901 tcagggaata agctatgcag ggcaggaatt gcaggtagtc cgctggtgtg tgtaagaaga
      961 gcaagtatag ggatttgtcc tgttttgtaa ttcagttccg gcaagtattt gccaagggga
     1021 tctttaagat ccaatgcgcc tgcttgcagt gctttcagga taaggagact tgtactcaaa
     1081 ggcttagtca tcgaagcaac atcaaagcga gtctcggcat cgatgggagc aattaccact
     1141 ttagaagcct ggtcatggtt ttcaatagat tgggcagcag caggttcttc tcttgctgta
     1201 aatccggaac aatgcgacca gaagagcttg ccatccatgg aaactgcagc cgcaatgcct
     1261 gtacagaggc ttttcgatag tgcatctttt atgaggttct cgagagactc ctcggttgga
     1321 ttatgcctat gtgaggagcc tttatttttg ttgtaggtca taaataaata tgttagcata
     1381 tatcatgagt aagtcaaaac agcaacgggc ttcagttgtg ttaggtcttg aaaactttct
     1441 ggatgatact cgtcgatata ataaacgcta tggattagct accaattctg cggcaataac
     1501 atccagaggc ataccttcat ggaaagcttt catagaacat ggaattattc cagcatgtct
     1561 ctttggaccg gagcatggat tcagaggcgc tgcgcaggat gccgtggggc tggatgatga
     1621 ggtcttcagg ggaattcctg catattctct atatggaaag cgcctcaggc cagagccatg
     1681 gatgttggaa tctttagatg cccttgtgtt cgatatgcaa gatgttggct gtcggtatta
     1741 tacatatcta tatactcttg cttatattat ggaagtatgt gaaaaagtgg ggacaccgct
     1801 tattgtgctc gatagaccca atcctatcgg aggatcgaag atcgaaggtt ctcctatttc
     1861 taaggaagcg gaaagctttg tgggtggcta tgggcttcca ccgcgctatg gaatgacaat
     1921 cggagaattt gctctatacc taaagggcga gtattatccc aactgtgagc tggaaatagt
     1981 caagatgtcg ggctggaatt cgcgtatcgc atgggaaggg acaaacctac cctggcctct
     2041 gccatcacct aatcttccga ctctcagctg tgccgaatta tatcccggta cctgtcttgt
     2101 agaaggcacc tggctttccg aaggacgggg aacctcacgg cctttcgaga tcattggagc
     2161 gccatttatc gatggagagg ctctgagaga acagcttgct gcttttgaat tgcctggtgt
     2221 ggtgttcatc tctctgtttt tctcgccttc ggcctccaaa tacaaaggag agctttgtga
     2281 gggtgtcttg gtttcgctta ccgatagaca agctctgcgg tcgctcgata caggcataac
     2341 tctggtccat acaattcatc gaatgtatcc agacaaattt agatggcggg aggattggga
     2401 tgataaaagc cttagctttt tcgataagct ggcgggtgga acaaccctgc gatgtatgat
     2461 aacaagcgga gcacctctcg aggactgtct cgaatttgcg cacagagatg agagtcaatt
     2521 tctgaggatt aggggaaaat atcttatata tggcgcccag aaggcctaag ctactcattt
     2581 gcttggggtt ttgctgcttg tttgtccgct tcaaagccct tgcagttggc cttttcctta
     2641 cttcaaggcg ccttccgtca gacctgctat gaattgcttg gaaagaatca ggaatacgat
     2701 cagaattggc aaaaccgata tattgagggc tgcaaagagc acatcccatt ccacagtata
     2761 ttcgccaaaa aatgcctgta gccccagagg gacggttttt ttggccttgg aactcagaag
     2821 gattaggggg aaaaagaagt cattccatac atctataaag ttgaacattg ccacagttac
     2881 gagagctggc ttcaaaagcg gcaggtcgat ctgccagtat atgcggaaag gtcccgcacc
     2941 atccatctta gccgaatctt caatatcgtg cggaaggttt ttaaagaaat tcacaagcaa
     3001 aaaaaccgaa aaagcaattc cgctggcagt gtatacaacc actagacccg aaagcttgtc
     3061 caagagatga agattgcgca tgatgaggaa aattgggata atcgcaaggc gtgtaggcag
     3121 tgcaagacca gctaacatgt aaagatatat tcctcgcttg aatttaaaat cgaaacgagc
     3181 gattacatag gccgccattg aagacacaat tatgatagag acaatcgaca aagcggtcac
     3241 gagaacggaa ttcttgaaat aagttgcaaa atcagcacgt tgccaggcgg agacatagtt
     3301 ctgccatctc cagacgcttg gaagactaaa tggctcaaga aatatctctt ttgtagattt
     3361 gaagctggat agcatcatga taccgatggg atatagattt gcaaacgcga gcagaagaag
     3421 aaaaatccat gctagcgcct tctggaatcc attaaacttg aacatcattg ccccttttct
     3481 acgcgttttt caacaataaa aacgtagaat atcgagactg gtagcactat gaggaatatc
     3541 agcgtcacaa cggttgcccc aagtcccatt cctacttggc ttgcgccaag tcctccaaaa
     3601 gcaattcggt agaaaagagt tccgagaacg tctgtagacc ccgcaggacc tccctgtgtg
     3661 ccttccatcg catatacaat gtcgaagata ttgaatgtcc agataaactt aagcatggat
     3721 gtagttgcga ttatcggata gagatgaggc agaatgatac ggcccgcaat ttgcagttcg
     3781 gaagctccat ctatgcgtgc agcctctatc atttctctgt ctacgcctaa aatcgcggcg
     3841 aggtatatga gtatatagaa tcctaagcct cgccatgctg taacaaagat aatcgttggc
     3901 agtgcagtct ttgtgtctcc aagccatggt tttgccaaga agtccaggcc aactaacctg
     3961 aaaagctgat ttattaggcc ccattgagga ttgagcaata gtccccaaag gaatccgacc
     4021 actacgattg caagtgtatt ggggaagaaa aagaccacct tgagccagcc tgttcctttg
     4081 atattcgcgg aaagcagaaa tgcaaagaac agtccgggga ctacagataa aatgagtgac
     4141 caaataaaaa acactacatt gtgccatagc gcattgtaga attccgatga catgaaccca
     4201 gtgagtatgt ttttgtagtt ggcaaggcct atgaatttct ttgggccaat gcctggccac
     4261 tcatagaaac tgaggccaag gctcgagaag aaaggatagg ctataaaaag cgaatataga
     4321 ataaaggcag gtagaagcat ccatagaaaa gaatattttc gcacagcaac ctcgtttcag
     4381 cgctatacgc aagcgccgcg gctttttctc agaataaagc cgcggcgcag tttttcatac
     4441 agctgcactc aatatatact tagaaagatt atttcttgaa agcagggtac catgtcgata
     4501 ctccatcctg caatcttttt gccagagcct ctggcgtcat ttgatcaaga tacatcgact
     4561 gcatgtcttt ttccagaatg ttgtagacac tcggcgttcc tgcatcaaag ggtgagccca
     4621 cccagtagcg gttttttgcg gctatggtgg tcattttttc atagcatttt accagtatcg
     4681 gcttcgaggc tggcattgtg acgccgttta tagccgtgat ttcgccggtt gtcgaggaga
     4741 atagctcacc ataagccttg gtaccagtat aattgagaac tttcaatgca gcttctttgt
     4801 tttttgaagt attgtttagg ccataagcgc catccatgta tgtatatgct tttgctggta
     4861 ctttcttatc ctttggcgga atagggaaat aatcgaactt caagtctgga ttcatcttga
     4921 gccaatttgt attgccccag acaccataga agaccattgc tgcctgttcc atggcaaaag
     4981 ctacatcctg ttcggcgctg gtgttggcca taaaatcctt ttggtagtat tttttaaggg
     5041 cattgaggtc tttgagcata tttacatatt ctggatctgt aaattttgcc ttgcctacgg
     5101 ttagttttcc tatccattca tctcccggat agctcacgcc aaccatggcg ttttgcatgg
     5161 caaggatcca cgcttctttg ccggtaacaa agaatggcgt tacaccattc ttctttaggg
     5221 tctcggctat ctggaccatc tcatcccagg tttgtggctc cttgagtttg tatttatcaa
     5281 agattgccct attgtagaat attccaatta tctggtttgc aaagggaacg ccccatactt
     5341 tattatccgt gcttataaaa ctcaacgata catcatcgaa gttggataga tcgaccagtc
     5401 catttatggc agtaaggtag ccgttgtcta gcaaggcttg ggtccgggca ccggggagtc
     5461 tgcgggtata catgatatcc ggaccaactc cgccctgcaa tgaaaccaat agcttagaat
     5521 catattcggt aggtgggaaa acctcatatc tgatggatat ttgctcacct tttgccttaa
     5581 gtgcggtctc gacccctttc catacatcga catcctgagc tctccaaccc cagatgctta
     5641 ctgttttctg ttgagcatac ataggagaaa gtattgctat aagcagcaat acgattgata
     5701 gttttttcgc catcgacagc ctccttgatg aatatgtcca ggtccatttt ctctatggat
     5761 ccatggtttt ttattagtat tgatgaattt attgcggttg tcaatcataa aatgaaatat
     5821 tttttctgtt tttcgacaaa tgagcttgca attctctcaa aaatgtgaaa aataatttta
     5881 acagaagagg cttgtccatg gacatagatt cggtcgatga atacctggaa cgcgcgacta
     5941 ccgaatcgcg caatgtgcgt tctgaacata tcgatgctct accggtgata gaggtcttga
     6001 ggatcatcaa cgaagaagat aaaggtgtgc cctttgccgt ggaaaaggcc ttgcccagca
     6061 ttgcagcgct tgtcgaagat gtcgtaaagg ccttcaatag tggtggaaga ctagtgtata
     6121 taggcgctgg tacctccgga aggctaggtg tgctggatgc ctcagaatgt ccgccgacat
     6181 ttggagttcc gccagacatg gttgtcgggc ttatagccgg aggagaggct gctctcagaa
     6241 attcaatcga aggagcggaa gatcatccag aaaatggcga aaaagcgctc agagatataa
     6301 atttttccgc acgggatgta cttgttggta ttactgcttc aggagctgca ccttatgtac
     6361 ttggagcgat ggagtacgcg aggaatttgg gtgcggtagt tgcggcaatt tcttgcaata
     6421 ggcagtcaag gactttcgat attgcacggc ataagatact tttagaggta ggacctgaag
     6481 tcattaccgg ttcgacccgg atgaaggcag ggactgctca gaaacttgtg ctcaatatga
     6541 tcacgacttc tagtatgatc cgcattggca aagtctacgg caacctcatg gttgacttga
     6601 tgcccgtaaa tcgtaagctc atagggcgat cgaagcggct tatcaggcaa gcaaccggtt
     6661 gttccaagga tgaagccgaa gccgcgtttc tggcttctgg aggcaagcca aagatagcta
     6721 tactaatgac catactcggc attggcgcag aacaggcagc cgagcttatc gagcacggcg
     6781 aagggcggct ttctgaggca atacgcattt attcagagag caagggagat agcaagcaag
     6841 aaaacaaagg ggaaagaaat gaagcaagga gcgctcgaaa caatagacgc ggcaattcct
     6901 aatcttgcgg aagcggagcg aaagatcgcc gctttcgttc aggaagctcc gcaaaaagcc
     6961 ttgcatctca acataagcga gcttgctagg caagccggga caagtcccgc ggctgtagtg
     7021 cgcttttgtc gtcatatagg cgtaggcaaa tacagcgatt tcaaactatg gcttgcaaag
     7081 gatgtgtacc aaggttgggg cgaaaaattc ctacccgatc tggagctgga atcgcagacg
     7141 cctggcgctc gcgctattcg cggtatgacc gaagcagtcc gacgtaccat gaattctctt
     7201 gctcagacac tcgatccgag ggcggtggaa gaagcggcgg agcgaatcaa aggagctagt
     7261 atgaccgcta tattcggtat tggagcttcg ggattggttg cggaggactt ccatcaaaag
     7321 cttacgcgaa ttggcatacc cagttcatat ctattcgata ctcatgcaca gattacggct
     7381 tcctgttctc ttcggcaatc tgatctggct ttcataatat catattccgg agagaccaac
     7441 gaaatgatag aagtagcggc tcaatcaaaa gccagaggct gttttcttat ttcgatgacg
     7501 atgaccggcc aaaacaggct acgggattat tccgatctta tccttgaaat accgacggta
     7561 gagaggatct atcgaagtgc agccgagttg tccagaatta gtcaactcgc ggcgatcgat
     7621 attctcttca atgttctcat ttcttacgat ctggacagcg caatttcctc tttagagcgc
     7681 tcgatgcaag caacgcatca tttttgagcc tgcctgtggg gtgcagcacg actgctcttt
     7741 gaattactga tccgacaaac gataaatctg ttgtgcactc tattccagac ttggctttgc
     7801 ggaaagtcca ttgttctgat atcatgaagc accgatgatt cgcatactat tagagaacga
     7861 tgagttcatc gttatagaca aaccagcggg ccttgcagct cagccgggag aagggttgcg
     7921 cgatgatgtc attacagctc ttgagcgcca gttcggatac agactgtttc ccgtccatag
     7981 gctggacaag gatacggcag gctgcatgat gctcgcaaaa gatgccaggg ctgctaacaa
     8041 atggtcgaat cgtatcgcaa gcagagagat aaccaaacgt tatcaagcct gggttgccgg
     8101 gctgcctaat caggaaaaag gaactataga ggatgctctg catgtaaaaa ggggaattca
     8161 ggccgcatgt actctttgga ccctgcgtga gacctggtta tttcccttac aagagcactc
     8221 caatcggcgg gctgatgtgc aagatggggg gcatgtccac aataatagaa taggggttga
     8281 tcggtatgag gcaaattcct caattgcagc cgcggaagcc aattttctaa cactatcgct
     8341 tttggatcta gagttaagca ctggccgtat gcaccagatt aggcgccatt tggcaggtat
     8401 tggtctgcca attatctgtg acgataagta tggcgatttt tcgctcaata aagctctgcg
     8461 aaaaaatgga ataagacgtc tcatgttatg ggcatacgaa ctcgtgctgc ctttgccatc
     8521 tcttgagtca ccaaatctac caatttttgc agctgaacct gctcatttca tctctctaag
     8581 gcagactctg gagcgagaag gaaggatact cgataaaagg aatacagagc aaagcgatga
     8641 gaaaaccatt tgagatttcg acaggtgcat ggtgggtggt tgtcgatggt cggaccattg
     8701 ccgtgccttc atttcatgag agctggctag cctcacatcc gggaattgca agtggagctc
     8761 ttcatacaat cgattttgtg gaaaaaagtg gctggctatc cgtgacgctt tactccgagg
     8821 ggctaatcga agttataagc cgtgatatcc tcgataatcg ccagcgcgag gctttgaggc
     8881 agctcctcga aaccaataga tccattatta gaaaaatggt gctttttgtt ccgtctatcg
     8941 atggttgctt taccgcagaa gatttgcttt tagatgactg ggagcgactc tggaaggaga
     9001 tcgaaacctt tgccgcaaag gagataaggc aaaacctgtc cgaagaaggc atggaagtag
     9061 acaccggtca accatgagct aggaatattc tcacaattgc tgcccagcga ggatggattg
     9121 cagatacgcc gcattcttgg gcgcataacc attggaatga ttgttaaatg caacaaatgc
     9181 ggtcttgctc tgtttggcca ttatacgtat aagtcgggct ctatccttca attcctgctc
     9241 agaatagcag tattcgtagc gactcttcgc attgccagac caccaaagat ctgtatttcg
     9301 accatgcaga cggtaataac aaatgtccga tgttatgata atcgattcag gaggcagacc
     9361 aggcagatcg ggtctatcga ctgttacgag tccaaccttt agctttcgaa gtgtttcaaa
     9421 aactctgtct tgataccagg tggcattccg gaattcgacg accagaggaa agtccgagag
     9481 cgctgcgagc aagttagcaa gatatttcct attttctatc gtataagaga atcgatatgg
     9541 aagttgcaca aggacacatc ccagtctccc cgtcgaagat agcgcagaga cggcaaggga
     9601 gaattcattg gcttttgttt gccaactttc gtctatctca tgggtaaggc tgcgatgtac
     9661 ttttaacgaa atgcggaaat cgctgggggt taggagcgcc agccgtttga gctgagcggg
     9721 attaggcatg cgataccatg tgttatccag ctctacgaag gaaaagacca gcgaatagta
     9781 tcgcagcatt tcattcgaag gcagatctgc cgggtaaaat acggttttcc attcctcgta
     9841 ggaaaatcca caggtaccga tatgaacatt gcctatatgc atcgattttt agtatagtag
     9901 gtattggttt ctaaataaag aaccgatgtg cggaggataa ttcatggtat tttcaaccct
     9961 tgctaagata gtgagcagtc tcgtatcgct atacatgatt ctatgcatag ttcgaatatt
    10021 tttctcttgg ataccttcgc ttaccgatac aaaatggggc aggcttattg ctggtctaac
    10081 ggatccgtat ctcaaccttt ttcgcggttt ttctcttttc cgcacgccgg ctatcgattt
    10141 tagtcctata gtggcgcttg cagtattatc ggtgctcaat aatcttttta tgacactttc
    10201 ttatgccgtg cgtataacac ttggttttat tctaagcctt ttgctggatg cggcgtggtc
    10261 agcaatatct tttttattgg gtttcttcct tgtgatatcg cttataagga tcataggttt
    10321 ccttgcgagg atagccgtgc ttcatcctct ttggcagatt ctggatggaa tcatcaaccc
    10381 tcttcttttt agaataaaca ggattatcta tcgcggtcga acggtacaat atctacaagg
    10441 tctcatcacg ggttttattg tggttcttgt aatgcgcact ctgggaggtt tcctcgtcag
    10501 gcttctcaca tcccttttga tgtcgctgcc aatctaggaa gaaggggcag catagatcgt
    10561 ggtgtacttt tagtggtgta cttttaatag tctttcagat cttctatatg gagagcaata
    10621 taggcacata gaaaattaaa gccaggatac gtaagcccgg ggtccagaag tccatcgctt
    10681 agcatgagga tagaattggt tattctaggg cttgcgagaa gtagccgcgc aagaggagca
    10741 gtatcggaat caggggcaaa tagagcatcc actgcagaaa tccatacatt caatctatca
    10801 tctccgagat tcagatcctt tgaatatgct atgagttgtg caatgccata ggaattgtca
    10861 tctatgaatt ccgaatatag aaggtagaac tccttatcga tgcccagaat gcctgatatg
    10921 ctcagtacaa gttcgtcgga tacactggca ggtctatcct cggctttaag acaggatatc
    10981 agtgtaggaa tagagtcaat tcttccgaaa agctcgagcg catacgctcc ggatattcga
    11041 actcgtggat tctccataga tcgcataagc tgttcgattt tgccaactgc ttcaaaggct
    11101 tggagtctgg caagggctat catagccatg ctctgcaaca tatagtctgg tccatccagc
    11161 gactcgataa gcgctggaat cgctattctg gatcgctgtt tgcccagtat ccgagccgca
    11221 acataggctg tggtatagac attagctttt acttctcgta tcaaggcgac aattgtctca
    11281 tgatcgagct ccggtattga ttcgagggcg aggagagctt cggatctaac ctcgaaacgt
    11341 ggcgaataga gatactccac aagttctttt tgcgtaagat tcgattccga cttgctgagt
    11401 ttttggatga tgcgcacttc ctcatgggca cttgcgctct ggtcgagtct cgtcaataga
    11461 tcgaaagctc gtagttctcg aggagaaagc aaaatagaga tcgactgagt taccgacttt
    11521 gcacccatgt gtggtagatt tcgcatgcta tagatcacaa taacaagccc tacactgatc
    11581 gcagcgtagt agagtctgaa ggctccagca tcagacaatt ccatattcgc aaagacctcg
    11641 agaattccgc ccccaaggaa tgagcccaga gatcctccaa tcccataggc tagattgtag
    11701 attaccgcca aatcgagcat tttttctttt ggcaccaggc caaaataata gacctgtgca
    11761 gcattctctt caccagcaaa tccaaaagag gaaagaaaat gaactatggc cagaaacaat
    11821 gctataaccg atggtgcgcc cattaaggtc gcggaaccag gaataagggc gattggaaga
    11881 aaggaaacaa atccgattgc gctatagatt ataaaaagtg gcttcggacc gaggcgatct
    11941 acgaccaggc gggttatgag ccccatcgtt atcgagccaa ggcttgctac gatcgaatat
    12001 accattatcg catcatcgcc ctgagcgaag acttcttttg catagaccgg cagaaatgcg
    12061 cgccccatgc cggaaaggaa ggatagcaac atgaaagtca atataaacgc gcgaaaatcc
    12121 ggctggacaa gagcctcttt tgtgacatcc cagagagatt gctcatcttt gccggaaaag
    12181 gcttcaggtt ccggcgtctt gaatagtatt gcacaactga taagcccggt aataattcca
    12241 agggctattg caattgtgta ggtccatagc gaggcttctt ggccaagcaa aaatgccagc
    12301 acaatgttgg ccagaattgc cgctactgag ttgacgacag atatgttgac tagatatcgc
    12361 ccttgatcac ttcgaggttt gtcgccacct ccggtggata atagtcccaa caccggatta
    12421 tttccgatca gggcaattcc tctggatatg tggaatcctg ctgtacccag aatgagtata
    12481 agaatcgcaa gacccatatg ccccttcgag gcgaatagag gggcaaacag aattggtact
    12541 agggcgatat accttataac ccagccccag ccgaatacct ttattatcga agaccttttg
    12601 accaaacgct tgcccagcgg cagcataaaa aaagttacat aggccagggc attcaaaagg
    12661 ccgatgatac tattcttagc acccaatttt agggcaaata gcgtgataat cgatccagat
    12721 acaaacacga aagagaaaga atttaaaaag ttgtaaatat tgaatgtctt gcgtgccttt
    12781 gcaagcttct gagcagaaag cagaatggcc atattgtcga atatatacta tagcaagagg
    12841 atgtgctact cgatactatc tcggataggc gcttgttttg ttatgaatgt tctgcccttt
    12901 ttgcttcgtt ccagaatgca tccatcatat ccatattttc ttgattcata tcgagatgct
    12961 gctgcttcat gctctcctcg acatgacgga atcgtcgcga gaatttacca ttggtgcgct
    13021 gtagggcgat cgatggatcg atgccgtaaa gccgggctgc atttattacg gtaaaaagaa
    13081 gatctccaaa ttcctcttcg agtgcatcct tgctcgatcc gctgtcggta gctatgtcag
    13141 atgggcattc ttcagatttc caggccctgg cttgcattag ctctcctaat tcctcttcca
    13201 gcttgttcca aacatctcgc tcgtgctgcc aatcgaagcc gacttttgcc gcttttttct
    13261 gaagcttata ggctttttct aatggcggca gactacgatg gacttcgtcg aggattgact
    13321 ctttttttct acggccttcg acttgctctt tgatttcgtt ccattgccgc acaacttgat
    13381 ccggactaag gacctcggac tcaccaaata tatgaggatg tcgacggatc agtttgcggg
    13441 cttcatcgca caaactatcg gccactgaga aacgtccttc ttgctgacac atgtagccta
    13501 ccatagtggc gagaagatat agatctccag tttcttcttt tatatgaaga ctgtcatctt
    13561 cggttatggc ctctgcaagc tcgtatgcct cttcgataat gttgccgcgg atactcgatg
    13621 gcgtttgttc gaggtcccag ggacagccat cggatgcacg aaggcgtgat accacaacat
    13681 agaaatacct aaaagccttt gcggtcctat ccgcatctgc aagaagagta tctgcgttct
    13741 ctcctgcgat atttctcaga gccaatgaat ctaaatccat aaatccctct tttgagttat
    13801 tcgtcgatga tatcaaaagc agcaagcttt gacataaggt ctattctgtc tactacattg
    13861 aatttatgat agatgtgagc aaaagtatta cgaactgtcg attcgcttac atggtgctta
    13921 taagctatct ctttaaaagt ggctccattg aggcaatctc ttaagaagtc catctctcgg
    13981 caggtaagat tcaaatctct tgggtaaatt ctataagcta gatcaacttt cccaatctgc
    14041 gatctatctt gaattattgg gatcaaataa ataaaaaaca gagataggaa ggcaatcgac
    14101 aaaattatgt cgaggaagaa ttctttatgc atgatggcga ccagcgcaaa attccaggca
    14161 tagtaggaga taaggatgca agctttgaat aggcctgcat gcttaaaaaa gcccaggcga
    14221 ttgagttcga taagcgcgat gataaaggcg caaatactgt acataaaagt caaatacctt
    14281 gtgagagtca atggcaaaag agcaataata aggacaggcc cgagccaggc taattttcta
    14341 ttaaggtgga caatcaaaaa cccaaaagag aatagcccaa taagtatgag catttctggc
    14401 gaatagtagg ttagaaaagc aatttcgggc atcaatatca tcaatataca acaaaaaacg
    14461 acgaacatgc tcgtaatcaa gaaaaataaa aagctagatt tttgtagctg caaaagagta
    14521 agtgattcta tttttgcaga tttttccgag gcaggagtcg actcattctt tggagcgggg
    14581 aaaccctgtt tttcaagatt cttcatcgcg attctccctg agtagagtat ttcctatttt
    14641 ctatttgata tcaaatatga tatctaattg ccgaaaaact cacgaaagca ttgtcgcgat
    14701 tcgcatgact aacagggctg cagcaatccc aattgcatcc gctccaaaat ccttcaaatc
    14761 gaaatgcctg gttattgtaa gaagctggcc gatctcggtc agaaatgcaa aagcgcttat
    14821 aattacgagg ctcaaaagga attttgggtt gattttcttt agagccctat gttcagcaca
    14881 aaatactgct gtcatggctc caaagaggat tgcatgtatg aatttatcaa agtcattaag
    14941 gctttgaggg gtttgttgaa atattctaaa tggcataaga agcaggagga gcactaccat
    15001 tgcgcttgca atggtccatt tatacgattt tattgtattc atcgagcatt gtcactttag
    15061 aagaaaatgg ccgcaaaggt caatattgga aagtaaaaaa gccaccccta attggagtgg
    15121 ctttcagatt tagaagcgga gcctatgaga tagagaccat tccagagatc agaaaggcct
    15181 gtccgctaaa tagcgatact cattatagcg ccgtccgata agctcgcgct gtttctccag
    15241 aagaaccttt gcgcgctctg ggttggcttg gacaaggctc ttaaagcgaa cttccgcgta
    15301 catgtaacgc tcgagcttag aagtgtcggg ctctctggaa tcgagctgga atgggttctt
    15361 tccttgtgcc ttgagcctcg ggtcgtagcg atatagcggc cacataccgc tttggacagc
    15421 cattttctgt tgatccatac ctttcatcat atctataccg tggttgatac aatgcgaata
    15481 ggctatgaca atagaaggcc cattatagct ttcagcctca cgaatggcct ttattgcctg
    15541 caccatattg gctcccaagg caatttgcgc aacatagaca tatccgtagc ttactgcgat
    15601 catgccaagg tctttcttgg aagtctcctt gcctgccgtt gcgaagcgag caattgctcc
    15661 aattggagta gctttagagg cctggcctcc ggtgttggag tagacttctg tatccagcac
    15721 aaggaggttg atgtttttgc ccgaagcaat tacatggtcg agaccgccga aacctatatc
    15781 gtaagcccat ccatctccgc cgaaaatcca gacagagcgg cgaaccagag cttcggctac
    15841 attgtcgagt tccttagccc agctttcgga tttgccggca agggattctc tgagtgcagc
    15901 cacatcggcg cgctgagctt cgatctgttc atcgctatcc tgagtattgg aaagcaacct
    15961 gtctacgagt tcttcgggaa tacccttacc tttggctgaa gcaagaagct ctcgcgcata
    16021 ttcattctgc ttgtccgaag tcaggcgcat gccgtatcca aactcggctg catcctcgaa
    16081 taggctgttg gaccaagccg gccctctacc gtccgctctc ttcgtatagg gcgtagtggg
    16141 taagttaccg ccatagatag aggaacagcc agtcgcattg gctatgattg ctcggtcgcc
    16201 aaacagctga ctcacgagct tgatatatgg ggtttcgcca caacctgcgc atgctccgga
    16261 gaattcgaat agaggctgct tcattgcgat gccttttgga atcgagaggt tcaggaattt
    16321 gctgtcggtc tcaggaatag agaggaagaa gtcccagttt tgtttttcgg tgtcacgaag
    16381 ctcgatctgc ggtccgaagt tgatggcttt tctgcctgga ttggctctat cgatagccgg
    16441 gcagatattg atacacgctc cacaaccagt gcaatcctcg ggtgcaactt gtacggtgac
    16501 tttgagacca gccacttcct tgccttttgc gtcggtgctc ttaaaggtgg aaggcgcatt
    16561 ctcgagtatt gcaggatcat aagctttcat gcggattact ccatgagggc agaccaggct
    16621 acaattgcca cattgagtgc aaagcgatgg ctcccataca ggaattttct ccgctatatt
    16681 gcgcttttcg tactgcgttg tgccggtagg aaaggttcca tcgagtggca gcttcgaaac
    16741 aggcattttc tcaccgcgct gcgcaataat ctcgcccaga gtttcccgca caaacttggg
    16801 agccgtctgt ggcaccggcg ggagcatgtg cttaatgctg tctgcttggc caatcttgac
    16861 cttatgcgtg gcattcagtg ccagatctat tgcatcgaga ttcttttgga cgacatcggc
    16921 gccttttcgc agataagtct tctctgtgta cttcttgatg agttcgacag cctgctcctc
    16981 gggcagtacg ccagagatct tgaagaatgc ggtctgcata atggtgttga ttcgcgtgcc
    17041 cattccagcc ttgtctgcga tggacatagc gtcgattaca tagaagctta gcttcttgtc
    17101 gattatttcc ttttgtactt ctatgggcaa attgtcccac acttcatcgg cagaataagg
    17161 gctatttaga aggaatgtcc cgccttcttt gatattggag agaatgtcta tcctctctat
    17221 aaagctgaac ttatggcatg ccagaaaatc ggcttttgtg atcagatatg gctttttgat
    17281 ctgcttcgga ccaaagcgaa ggtggcttat cgtgtaggtg ccggcctttc tggaatcgta
    17341 gacgaaatag ccctgagcgc agttttcggt ttcatcgccg attatcttga tggaattctt
    17401 gtttgcacct acggttccat cagatccaag cccataaaac agacactgaa taatgccatc
    17461 gcctggtaag tggaatttct cgtcataggg caggctcgta aagctaacat catcgtcgat
    17521 accgatggtg aagtgattct tcggatgctc aagcttaagg ttgtcgaata ccgccttgac
    17581 cattgcaggt gtgaattcat aggatccaag tccatatcgc ccgcccacca ctataggcca
    17641 attcttgaaa ggtgcggtat gattgcccat tgcttcgcct attgcggttc gcacatcttc
    17701 gtaaagaggc tcgccaatag cacctgattc ttttgtgcga tcgagaaccg ctattgactt
    17761 ggtcgttgcc ggtattgcct ttatgaaatg ctcgacagag aatggcctaa agagatggac
    17821 tttggtgaca cctacctttt caccttgttt cgcaaggtat tcagccgtct cttcggctgt
    17881 gtcggcgccc gaccccatga gtattatgac gcgctctgcg tctggagcgc ctgagtagtc
    17941 gaagagcttg taggctcgac cggtcatttc ggcaaactgg ctcatcgttt cttcgacaat
    18001 agcaggagct gcctcgtata gcttgtttat tgtctctcgt ccttgaaagt agacatcggg
    18061 attttggctt gtgccgcgaa tcacagggtg ctcggggctg agtccacgag cacgatgctc
    18121 tcgcactttc tcttcgggaa tcatggctcg tattatgtca taagagagtt cctcgatttt
    18181 ggaaacctca tgggaagtgc ggaagccatc gaagaaatga aggaatggaa cgcgtgcttt
    18241 gagtgtagca gcgtgagcga tgagggcaag atccattacc tcctggacat tgttggaggc
    18301 aagaagagcc cagccggttt gacgggtagc cataacatct tgatgatctc caaaaatcga
    18361 gagcgcatgc gctgcaatag cgcgggcggc aatatggaat acagtaggta ttgcttcacc
    18421 tgcgatcttg aacatattcg gaatcatcag caaaagaccc tgagaagccg taaaggttgt
    18481 ggatagcgca ccggttgtaa gtgcaccatg taccgcaccg gccgccccgg cttctgattg
    18541 catctcgact acctgcggaa cagtacccca gatattattt ctgcccattg aagagaactc
    18601 atccgcaagc tcgcccatgg gcgaagaagg tgtaatcgga tagatagcga tgacatctga
    18661 acatgcgtgt gcgacatgag ccgcggcggc atttccgtcg atcattacca tgttcttgtt
    18721 attaggcatt gtggtattcc tctaagatat aagatattag aaatcggtct cgaaaccgga
    18781 cccactctat cctacaacca ttttatcgat atttcaatac tgctacgaac cttgtttata
    18841 aggattgaga atggcatatg aaagccttct tgtgcgtaag tatagccagg tacataatcc
    18901 gctttattct tatatgtaca agacaagtcc atgcagcata ttggacacat tccttgcaaa
    18961 gcacagcgtt tctctgcgat gatccgcaag gcactttcta aactctcttt tgagatagta
    19021 aacccggggc gcttcaatag attgaattgg agctgcctta tgatggagac atttgggatt
    19081 agggcagaaa gggaaagtct gcatagttag ctccttgctc ttcccttatt cgggctatta
    19141 cctccttctt acttcaactt cttatacaca aggttcgtag cagtagtgtt ttgatatcta
    19201 ttagaagcca gaaccgggaa taatagtagg tggtgctaag gtatatgatg gttaagaagg
    19261 cggtgtcgag ggacttgagg ttgtcgatac tgcatacgct tcttgggatc cttctcattt
    19321 ataatatagg ctccattggt cacatgatgg ggtaaccaca atcgtacacc cgaaaatcag
    19381 catgcccatg ctcagcgcca agagaatagc ttttgcttct tctttaattc cctaatcctt
    19441 gaatagtcga ttaaaagaac caataataaa aatgtatcga agggctttta gaataaaagc
    19501 caaattcgcg gcgatatctt gcaagatgtt attgcttagt atttgaagct agtatgaatg
    19561 acaggtcagg taagtctaat attgatctac agttcaagca tttttatggt ataagaaatg
    19621 caagggagag caaggaaaat ggtcattcag gataaagaac agcttgaaaa actctttgaa
    19681 tcacaggcgg agcggcgctg gaaactagcc cttactgggc cagaagaaag gattgtcaaa
    19741 ttaaaaaagc tccgctcggc gatactatca cgaagcgacg aattatatgc ggccctgaac
    19801 tccgattttg gtaagcctgc atttgaagcc tggttaactg aggtttttcc ttgcatagaa
    19861 gaaatcgacg ttgcagtacg ccatctcaag gtatggatga aaggaaggcg cgcaaagggg
    19921 actctcattt tgcctttttc ttctacgcgt gtgcgatatg agccaaaagg gcgcgtcctc
    19981 atcatggcgc catggaacta tccgtttcag cttctcattg cgccacttgt ctcggcggtt
    20041 gctgcaggta actgcgtgat cgcaaaaccc tcaaataaga caccgcatac tgctgcgttt
    20101 atcgcagacc ttattgccgg agtattcgat tcccaagagg tagcgattgt tgagggttca
    20161 ggcagcacgc ttggcgattt tctgctagaa ctcacgttcg atcacatatt ttttacagga
    20221 agccctgcag taggagtcaa ggtaggtcag gctgctgcgc gcatgcatgc ggggctcacg
    20281 ctggagcttg gtggtaaatc gcccgccatt ctgttgcgag gcgcgaacct aaagcaagcc
    20341 agtaagcgaa tcgcttgggg caaatttctc aatgccggcc agacatgcat tgcgcccgat
    20401 tatcttttct gccctatgga gttggttgga gaatttttag cagagatgcg cctggccatt
    20461 gaatcattct atggaaagtc cgaagaagag aggcaggctt cgccagatta tccacgcatt
    20521 gtggataagg ctgcctgtgc tcgtctcgag agaattgtct cagaggcgat ttccaaagga
    20581 gccaagcttg aattcggtgg tcgcttcgat gtggagaatc gatatgtttc gccaaccctg
    20641 ctcacagggg tgcaaatcga tatggccatt atggcggagg aaatctttgg tccaatactg
    20701 ccagttcttg cttacgattc actcgatgaa gcaattgagt ttataaggtc taggccaaaa
    20761 ccacttgctc tctacattct aggaaaagat aaaaaaagcg cagagaggct cctggcggaa
    20821 acgacttcgg gcagtgtcgg cataaatgac cttattgtgc agatcgaaaa ccttgatgct
    20881 ccttttggcg gcgtggggat gagcggaaca ggaagctacc atggtttcta tggctttaag
    20941 actttctcgc acgagagaac ggtattatat cagggcccca taagcgcggt tgaaaaattc
    21001 tatccgcctt ataatacttc gacacagaaa aggctgctcg gagccctgaa gaagatcaag
    21061 aatatcagct aaaagtccga aaatatcaca agctagcagg gatatattgc taacctagct
    21121 taagtgtttt ctttccggtc tatttcgatc aggtttcttt ccgctatgcc tttgatatag
    21181 acatcgcgct tcggatacgg tatttcgatg ccttcttcgg caaatcgctc ttgaatatcc
    21241 attagaattg tattctttat ttctacgaga tttgactttt cgaaccatac accgagaaga
    21301 atacctacgc ccggaatatc gaatttgtct ataagaatga ggggagatgg attatcgagc
    21361 acatagatgt ttttgtatgc aatatctcgg agaacatctg tggcttttct tagatcggtg
    21421 gtataggaga catttacata tatatcgagc ctgcggattg gaaagcgggt gagattgatc
    21481 acgtttgtct ttattatgct ttcgtttgga atccggacaa agcggttatc gaaggtctga
    21541 agcttgaccg ataagaggtc gatagacatt acgattccgg atatctcatc gatctgtatg
    21601 gtgtcgctta tttgaaaggg tttttcaaaa acaagaaata taccgctgat aaggttcgac
    21661 actgaggtct gtgcagcaaa tccaattgcg attcccgcaa ttccggctgc tccgagcaaa
    21721 gccgaaagat tgacgcctac actctgcaag acagatacaa cagcgattgc ccagcctgtg
    21781 tagcggagag cttttaagat gagctgtgct ttttgttcgg gtatagtctt tttcatccca
    21841 cggcctagaa ttatccgtag tattctgaaa aagacaagaa ttagaatcgc cgtgatcagg
    21901 atcgtaaaag ccttgctcca gaactcgggc gtaagggcat cctgtataaa gcgatctatg
    21961 gaatcaggga aaattgtgtc atccatttcg ttctcctcat taaaggccgg caatactgcg
    22021 caagaaactt acgcctttca taaccagcag ctcattcagt ccggtcttag gggctgcatg
    22081 aatctttgga agattcttat aagcactatc atcgatattt gtctgcagcg aatcatccgc
    22141 ggcagctgtg attatgacgg tatcggacac gagattatct tgtttctggt agacattcat
    22201 gaggcttgta aaaatggcaa ggcgttttac atttagaggt tctttggatc tgtttttgac
    22261 aacgattttg caatgaatca gcgagcggca cgataagtat tgagcggctc tctgaagcga
    22321 ttcctggatt atgggatcga gagagatagg attattagag tctattcttt caaaatagcc
    22381 ttgttcattc ttgcactgag gatctagctc gatgggaagg gaaaggcaga gatctccgct
    22441 taccttatcg ccaaaccagg tataaggggt tacaaatgga agactttcga agagagacga
    22501 tccgttttct agttctatcc gaaatatggg cggaagtgcg atatcgaact gagcttcggt
    22561 cccagggtaa attttgacat cgtttcgcgc cgatacgagg taaggtgcag tacttggatg
    22621 tgggcgcagg gctgcttttt tgccaggagc gaccgcaaag gcgatcgata gctcgcttgg
    22681 agcatcgacg aaaagagggc cagccgaatc attttgaatc tgtttgaaag ggatcgagct
    22741 agcggcaaac cgccattctt ctccattttt gcgcatccag agagaggctc catcgagctt
    22801 ccagcaatac cattgcgact ctttaggtac gaaaaaattc cacatacgat ccataatatc
    22861 gtatgtggaa tattccataa agcggattga gaagaattcc cactattttg ttgcgccagt
    22921 tcgagcttgt attggcgaac accgcttcaa atctgaagtg atgtcatcag ggctgaatca
    22981 atgccgactg ggcagccttg agcgaggcgc ccggttcgaa ggaatagccc gctgtcgcaa
    23041 gaccttgctc tatagagcca agcactgcaa gtatctctga agcatcgctc atgcccatgt
    23101 ggccgattcg gaaatatctt gtcttgattg ctggatgcaa gcccccggcg agcacagcgc
    23161 cattctttgc aattaagggc aaaatacttg cgtcggtgcc ttcggggtaa tatacggcac
    23221 tgagagtatt agccattatc tctttacgca cggggacaaa gctaaggcca atggctgata
    23281 gccctgctcg gaaagccgcg gcgtttcgcg catgcctttc aaacctggcc tccatgcctt
    23341 cgtctagaat ctgtcctagg ctgatattta gtgctcgtac aaggtttaca gcgggcgttc
    23401 caaagtaagc cgattttcgc ccttcgtatg cttccatgac gggcagccag tattgccagt
    23461 cgcaataata ggcacctacc gggctttttc tagacctaaa agactccatg gcttttttgc
    23521 tggtcacaag gagcgcaagg cctggaggaa cgccgattgc cttctgtgaa gcggtaaagg
    23581 caatatccac tccccattct tccatgcgaa gctcttctcc ggctaccgcg catactccat
    23641 ccactactac aagcgtgttc gtctcctgtg cggctgcgga gagtgctttt acatcggtgc
    23701 gaactcccgt gcttgtatcc acatgggtaa tgctgagtat cttgtaattc ttagatttga
    23761 gtctcctgcg cacctcatct atttccggaa tatcgccgac cgcactttga actatatcca
    23821 cattagcgcc atgcagcctg aaaatgtcgg ccatacgttc gctgaaataa ccagtgtgca
    23881 caataagcac attatctcct ggttctgtta tatttgctgc tgcaatctcc atggcaagtg
    23941 taccacttcc agcaattgca aagggctgac cgttgggggc gagccagact ttccgcattt
    24001 tctcgagtgc ttgaccgaat tcttcgatga attgctgatc ggtatggctc agtgtcggct
    24061 ttgccatttc cgctagcacc tcaagggtaa attctatggg gcctggaatc atcaataagc
    24121 gttttccttt catagcaaac tcctctacta tagattgttc tttatattgt ttattttagc
    24181 atgattgttg cgctaatgtc atgagcgatc taggaatgag gaacagggca tgaccggcaa
    24241 aggggcttct acaaagacaa agtggaacaa gaaaagaaaa cttgtcgtat gtgatctctc
    24301 atttcggcga aaatgccgtc agagcaaatt ccacctcatt ggcgagacga tcagcatccc
    24361 ttttgatctc ttggctgaga gcggaccatc gaagggatcg tgcttgagca agttcttttc
    24421 tccagggcaa acttgcaaga agaggcaatt ccatccgctt agctccattt tggctgccat
    24481 catcgaatag agcaatctct gcaccacaat gaggacaaac cattgtacgc atgttttcaa
    24541 ccacaccgag cacaggcgtc ttgagcatgg aggccatatt gaccgatttg cgcactatca
    24601 tggtgacata atcttggggc gtagcaacca ccactaagcc cgagaaggga atggtctgga
    24661 atgcggtgag tgctacatcc gaagtacctg gcgggaaatc gatgattaga taatccagat
    24721 ctccccatat tgtatctgac cagaactgat cgatgacctt ggcgagcaag gggccgcgcc
    24781 atataactgg atcatcctca ttttcattga aaaggtttat ggagagcacc tttattcctt
    24841 cctcattttg cacagggtag aggttttcac catcggattc accgcgaaaa caatctatgc
    24901 ctaacagccg gggaatgctc ggccctgtaa tatctgcatc caacatgcct acttttaggc
    24961 cgcgtgcggc aaaggattga gccaggagag atgcgaccat agatttgccg actcctcctt
    25021 tgccgctaat tacaccgatg acctgcttga tagcaggatt tggctttttt tcgggaacaa
    25081 gatccatgaa tgtatactcc taaagaacga agctctgaat aaatggaata tggcatgata
    25141 aatgatatta ttcaagcata atgtttttca tatgtaaaaa agctcatgtt taacggttgt
    25201 atagaggtag acaggcttac tccttccatc caaagcgctc tctaaggaat gcgagaaact
    25261 gagaccattc tgtcttggat tcgaagaact ctctggttgc cctgaaccct gagtctatga
    25321 gcattctatg tcgttcatct cctggctgaa tgtcgaaatc cgtggcttcg ataccacaat
    25381 cgtcgatgta aatggtccgg gaataattct tctctgttcg aatggcgagt tgctcttgca
    25441 ccgtcgaaac actttcaaaa agtgatctca tatagtcgag catggatttt attggaggat
    25501 agtgcgtctt ttcggaagaa gaaaatagaa agagccctaa agtctcggca tttgccccat
    25561 cattgagctt tctgcaatac ttatgatcgt cgaatagatt tattgggtaa ttccacataa
    25621 cgccgccatc gacatagaat tgaggctctt cggtgcccgg atactggtac tggatagctt
    25681 cgaaaaaaag cggaatagac atcgagatac gtactgcgag agcaacttcc atatctgggg
    25741 ttgtttctag cgaaaagagc cgagcagtgc gattggatac atcggtcccc gtaatatata
    25801 agtctaggaa gggtctgccg tctctatgga tttgtgtatt ggcaaaatcg gcgaaagtat
    25861 atatctcctt ggcgacagtg aaatactcgg cgatgataga tctaagccat gtatagaggt
    25921 attcgcttga ataccagcct ttttcctgca aaagtctgat ggaacactgc aagttcttaa
    25981 aaatggcaag ctcgcgatag tgctcgatta ttgcgatgag ccgcttactt gtgagttttt
    26041 cggtattctc gtcctgttcc gaaggaactt tgcgataatc gaggctatcg gctagcgttc
    26101 tgaagcccat aaaatctgtt ggattcattg caacaccgag cgcggttatt gctccggccg
    26161 atgtaccggc aactctccgg atatgaggca ttatacctcg ctcatatagg tattgaagcg
    26221 ctccgatata tgcgatgcct cggactccgc cacccttgaa gaccagattc ttataatgcg
    26281 acataggcct tttcttctat gttctttgcg ttttgcggca ttcccaatgg tccgctcata
    26341 ttgtctttaa attcatgaga aaaatcaatt ttcatctggc tctttccaaa accaaaagat
    26401 ataggcaact cgaaatacag tcaatgttag gcgatcaaaa aaggcgattg tccaataggc
    26461 agcctgtatt ttattacctt gtttcggata gaatagcgat tgaagccgac cgggactaat
    26521 taaaagagcg ataaaagata atagcgaata gagagaatgg aggcctaaga ggtatctatg
    26581 aatatcgaaa atcatgttat gcagaccttt tttgcaaccc tctttacttg gggtatgaca
    26641 gcgcttggcg ctgcgatggt cttctttttc aaggagatca acaagaaggt tctcgactca
    26701 atgctcggct ttgcggccgg agtgatgatt gcggcgagtt tctggtcctt gctttctcca
    26761 gccattgaat tatccgaatc tatggggctt attccgtggc taccagcggt tattggattc
    26821 ttgcttggag gagcgtttct actaggggta gaccatcttt tgccacattt gcacattgga
    26881 gcggatatga gcgaggcaga aggccttcat acgagctggc aacgcagcgt ccttcttgtg
    26941 cttgccatta ctctccataa tatccctgaa ggtcttgcgg tcggcgtggg ttttggggcg
    27001 attgcggctg atataccaag cgctagtttg tcgggtgcaa ttgcgttggc catcggcatt
    27061 gggttgcaga attttccgga aggggcagcg gtctctattc cacttagaag agaagggctt
    27121 tctcgtcgaa aaagtttcgt ttatggacaa gcatcgggtc ttgtcgagcc catagcggga
    27181 attctgggtg cggtccttgt gctcgcaatg cggcctattc tgccttatgc gctttccttt
    27241 gctgctggcg cgatgattta tgtcgtagcc gaggaggtgc ttcctgaagc aagtcgcgat
    27301 tcaaatggcc accttgctac cataggtact atgtgcggat tcgctcttat gatgcttttg
    27361 gatgttgcgc tgggttgagt gggcaaggag gctgacaaaa ttcggattag ccagataagc
    27421 aaggcaaagc atgattaggt agggattgca aaacgaaaat gatgctgcct ttagctctca
    27481 ccgccagctc tcgcaggttc tcaccgccag tatggcagct tcaactgagc actttcccta
    27541 tggcgctgga cattgaatat tcttgctctt accacaaggc acccgacatc tgcgttttag
    27601 tcgtctcctt tcataaatgg atagcgccag tcggctggag gagagaaatt ctctttgatg
    27661 gcgagcgcgc tcacccatcg caagagattg attatgctgc ctgctttatc gttggtgcca
    27721 gaagctctcg ctcccccaaa aggctgcctg ccaaccactg ctcccgttgg cttatcgttg
    27781 atataaaggt tccccgccga attttgaaac gcaagaagag ctcgattgat gcgctctcta
    27841 tccgtagcca ttatggcacc tgtgagccca tagggcgaag tagaatcaat aagcctgtaa
    27901 gccgcctcca tgtctcgatc atcgtagaca taagccgtaa ggacgggtcc gaatatctca
    27961 ttgaccatag tctcggagcg cggatcctcg gtgagaataa gagtaggctc aataaaccaa
    28021 ccgtgagatt tattgcaatt gcttcctgca agcaccttcg ctccttgtgc ctttgcgcgt
    28081 tcgatatagc catgaatatt ttcgaaggct gtttcatcga tcaccgcacc catgaaattg
    28141 cggaaatcgg tgatctctcc catgggaatt gtagagatct gggcaacaag ggcttctatg
    28201 aattcagaag cacgcgattc agggatatag agccgagatg ccgccgagca cttctgacct
    28261 tgatattcat aagctcctct gagagtggaa gctacggtag gttcaagttc agcgctcgga
    28321 tcgatgaaga taaaatcttt gcctcccgtt tctcctacaa tgcgcggata gctccgatag
    28381 tattccagat tcgatgaaat ctgtttccag agagagcgaa atacttctgt cgagcccgtg
    28441 aagtgaaagc cagcgagctc aggacttgca agcactacat tcgaaatcag actgccgctt
    28501 cccggcacaa agttgatgac tcccggaggc aaaccggctt ccatatacag cctcatgagc
    28561 atgtagttgg agtacactgc attagatgcg ggcttccaaa ccacggtatt gcccatcatt
    28621 gcgggagaag tggggagatt tgcagctatc gatgtgaagt tgaatggggt tacagcatat
    28681 acaaatcctt ctaagggcct gtaggccgat ctattccatt ctccttttac attatctggc
    28741 tgctgcgaat agatttgctc cataaaccag gggttgaatc gaagaaaatc gattgcctcg
    28801 cagacagcgt cgatttcggc ttggtaggcg gttttggact ggcagagcat ggttgcagca
    28861 tccataagcg ctcgatattt acccgaaatg agttccgcaa cttttaggaa aaccgcggcg
    28921 cgctcctccc agggcatggc agcccatcct tcttgcgcgg caagagccgc ttccattgcc
    28981 atcttgactt cggtttctcc tgccagatga tagactccca ggacttctcc atgggcatct
    29041 gggcggcgaa tagtagctgt cttgcccgta cgcagctctc ttccgcctat aatcagggga
    29101 atttcgatct gatttgtgcg gaggtgatcg agctcctttt tcaatgtggc tcgttcaagc
    29161 gaacccgggg cgtaattaaa cacaggttcg ttgattggcc ttggaatgtg gaatattccg
    29221 ttattcatga gtaactcctt ttatttcatt gtgaaggcgc tcaaaggatt cgtcaagtca
    29281 gatctttata acacttgatt tatgcggctc aatgattcta ggatggaata agagaggtgc
    29341 atgatggagt agaccaagac tttgaatgct tttatgctga tagtgctggt ctttattttt
    29401 cttgccggga taatagaggg cggaacacaa gccatcctgg gttttctgcg gctgcagatc
    29461 acacctgctc ggcttgtaag cgactttata gctattgcgg gagtagggtc gacactcctc
    29521 aatgtcagta cggttggctt tctgggttat ggttttctgg caattaataa actcaggtta
    29581 actggtgctt ctcttgcagc cctttttacg atgatgggtt ttgccttttt cggtaagacc
    29641 ccattcaatt gtcttccaat aatgcttgga gtatcttttt ctgcgctttt ggtaaggaaa
    29701 aaacctagag actacgccct catagccata ttcggaactg ctatgggtcc ccttattact
    29761 tttattgcct tcgagctcgg agtaaagagc tttcttgcct taccggcttc ttttgctatc
    29821 gggttgggcg tcggactcat ccttccgccc attgccatcg ccatgcttcg gcttcatcag
    29881 ggatacaatc tttacaacat gggtttgacc gcaggttttt tggggctttt tgcggcttcg
    29941 ctttcccatg cgacgggcgc agacattcta cctattgaaa tatgggggac ggctcatagt
    30001 ccaattcttg tagctttgtt acctattgtt ttgctgattg ctcttttttg cattgtgaag
    30061 gaagatccca agaacattgt cgctctattc cggcatgcct atttggattt ccgcaagatt
    30121 ctaggcatga gcggcaggct tccgagtgat ttttccgact tcgtatcgac aaagggtgcc
    30181 tttcttaaca tgatagtatt aggactgttc ttctggctct ttatgctaat tatcgaagct
    30241 cctttcaatg gacccgtctt gggagggctt tttacgattc ttggtttttc attctttgga
    30301 aaacatatta agaatgtttt tccgattgtg cttggtatcg tggtggcaat tttcgtattc
    30361 gataaggatt tatatgagcc ggggccgctt ttggccatcc tatttgggac ggcactagcg
    30421 cctctttctg gagaatttgg gcctttatta ggctttgttg cgggattctt gcatcttgtt
    30481 attgtggata gaacatgctt ttggcacgga ggtatggctc tctacaacaa tgggtttgcg
    30541 gcggggctta ccgcaacttt gatcgtttcg gttatcgatt ggtatcggtc gagtaaggtc
    30601 gcaaacaagg ttgaatgagc aaatccaggc cgattcaggg cttcttaagc caagcaagag
    30661 gagaatgagg atgaaaaggc acgatctata tgtacaactc ataagcgagc ttgctaatta
    30721 tcttttgcgc aacaatccga aaaagatggt gatatctctg catctcgaaa acgatggttt
    30781 tcatatagtc gctatagatg atcggtacca tagcgatgaa gagatcgcgg aaatagagca
    30841 aggtttgaag atgcatcaga gaccggaact tgcctcttac tatgggacaa tgctaggccg
    30901 agatctgtta ggcgacgccc ggctcgaact catcggctat catgtcaagg gtgcaaaagt
    30961 ggaaaaaatt cccgaagggg gtattatgat aaatctctgg gtaggatcgg atgattttaa
    31021 tcccgcgatg tttactattg atgaaaaagc atagaaaatg tctatagaag atagaacgaa
    31081 ataaagatgg cagtatcaat tctatggtat gatctcgaga cctttggtcg caatcctagg
    31141 cacgatcgca tcgcgcagtg cgcttttata aggacgaata tagcgctcga agaggtagca
    31201 gagccagtcg ttatgtactg taggcttcct ctcgattatc ttcctgatcc cgaggcatgc
    31261 tatatacatg gcattactcc tcaggaagcc gaagagaaag gcttatccga atatgagttc
    31321 tctcaaagga tacagcgcga aatgagtgtt ccaggcagca cggtgacagg ctacaactcc
    31381 atccagttcg acgatgagtt cattcgcaat cttttctata gaaatcttat ggatccctac
    31441 agtcgggagt acaagaacaa caattctcgc tgggatgtaa taaatctaat gcgagccgtc
    31501 cacgatctca aatatgaagg tttcgagtgg ccaataaacg gcgagggcaa cccattgttc
    31561 aagcttgttg atcttgccaa agcacaaaat gttgcccatg agaatgctca tgacgccctc
    31621 tacgatgtaa gggcgactat aggccttgcc cgcaaggtgc tcgagactta tccaaagctc
    31681 ttcgaatggt attaccgcca ccgtcggcgc gagaacttag cgcgccttgt agatctctct
    31741 cgccgggaga atatgctcgt acacacttca cagcgctata cgagacaagg tggttgttcg
    31801 acggtaataa ctcccttagg gctactggaa gatgatcgcc atgctcttgt gacctatgat
    31861 ttgcgcttcg atcctaagcc ttttttatat atgtccgaag atgaattgcg cgacctttta
    31921 ttctcggaga ggcaggaaga tgaaaagatt cgtccacctc tcaatatggt tcgcctcaac
    31981 agctgtccct accttgctcc tctcagcgtg ttaaatcgac aagctcgagc gcggctgggt
    32041 atcgatcttg tggaatgcag agagaatctg cgtattctcg aggcaaatcc tgatcttaaa
    32101 gcgcgcttca ttgctgccta tcgatttgag agaaaatcgg ttgatgagct tgatcctgaa
    32161 catgccttat atgccggcgg attctattca gacgaggatc gcagagaact ggagcgtttc
    32221 catagagtgt taatgaatag cggtccacag gctgcaaaaa acgagtttct tcgcattcga
    32281 tttcaggatc caagaattcc aaccctaatg ggcaggcttc ttgcgcgcaa ttttcctgaa
    32341 accttgacgg ctgatcagca tttatcttgg aagaagcatg tatacggatg gctacagctt
    32401 ccacaagaaa aaggagccac cgccctttcg gattatgtga gattggagag agaattgcag
    32461 gaaatgtctc cggatgatcc gcgaaggcct atagctatgg cgcttattaa atggaaggat
    32521 aagctatgca gtcaagccat ctgaagattg ttttgctgcg tgatatattc ttatatgtat
    32581 aggtgggtgc gagtagcaat gtatgcgttc gggctcaaaa tgaaagccgc agatgatttt
    32641 tcgtctttct gctcgcatat caagaaatga agtatgatag gaggctggcg ctggttgcgc
    32701 cgttatgggc acccagacgt catcaagaag gtgtgctatg aaatatcgat ggttttttgc
    32761 gatttttctg gctcttgcgg gtttgacaat tctctcggcg caggaaaagt ccctggagag
    32821 caccatgctc atcagggtcg aaaaagccgc agttagagcc gagccttctt ttgtttcgcc
    32881 ggcgctctct tttgccgctt acaagactcc agttgccata atacgcatcg aaaacgactg
    32941 gatatttggc tttgttcggg gactaaatag gccgggatat atccacatga gtgcactttc
    33001 tccagtcaaa gtctccttga accctgacga tgtctccgat ccacctccac tgcaggaaac
    33061 tgagattgtc cttgctggca agggcttttc atctagtctt gaattgtcgc tcagagaatc
    33121 aagtgctttc aacttcgaag cagtggatgc aatggaaaag aatagctatt cttatgcgga
    33181 atgccttgca tttatccatg gcatagatat tttggaagga gaaccatgag gtgcggaaga
    33241 ttgtgcttca atctcgcgtg ctatcggtta ttcttgcgct gattcttatc ctaccgtttt
    33301 tcgcatcttg caccataacg ccggagtctc ttcagttttt tctcgatcaa gccttgcttc
    33361 agggcatgat tacgcccgaa caatcgcggc tcattttgga agcggtgcgc tcttttcaaa
    33421 tcgaatctga accttttacc tatgaggaag aatacgttat agggagagta gtagccgccg
    33481 ccgtgctgtc tcaatatcaa gtttatgatc agcctgagct aacagcctat ataaataaga
    33541 tagggcaagg gttggcattt tattcagcca ggccatgcct gccggaggga tatcatatat
    33601 tggtcctcga cagcgaagaa gcgcatgctt ttgctgcgcc gggcggattc gtattgataa
    33661 caagggggct tctgaagctg acaggcaatg aggacatgct tgcggctctt ttagcacatg
    33721 agatatctca tacagcgttg ggacacggat tattggcact cacgagctat cgcatggggg
    33781 atttagcaaa atactatgta atgctttcca cagagagcgc gcagtcttac aataaggagg
    33841 ctatggagct gttcttggcg gcggtgcatg attttgtttc cctgatatct gtgcaaggtt
    33901 attcgcaagt catggaattc gaagcagacg ccgaagcgat gcgcatgctt tacgaagccg
    33961 agtatagacc ttcagccctc ggagattttg tagtaacgct ggagaattct aaggatgagc
    34021 gttttcagca gtattcgatg cagcatcctt tggccgaaga tagactatcg agaataggca
    34081 gccttgctga ggaatattct caacgagccg agctcgaaca ccgcaaagaa tgcgtacaaa
    34141 gactggattc gcttctgcaa aacgaagatg agtcgtggca taatagtaat ctagcttgcg
    34201 aacaaggctt tcttgattct ggatatgagc tcgcaaagga gaacttaatc atcagccctg
    34261 tgtctctcga gctcttgagg caagagcgct tcgaagccat tcggcctcta ttctaggcaa
    34321 tctcaaagta ttttagaatg aaatatatga cagaggcagg caagcccgac cacagcactt
    34381 taagatcgaa tcatgctttg aataaagtaa agagattttt atatgcgcta gtagccggtg
    34441 tctttggaat tcttttgttc cagtggacag ggatttccaa taaggctggt atggccctct
    34501 atgatcggga gatgcgtttg ctggtagcgg cggaaggcaa gaaggaggat aatacttcct
    34561 cccaaacttt acgtaagaat tccggttcgt cagattctgc atccctttca ccttccagga
    34621 aaaaagcgcc aattgcgctt gtcatgatcg accaatccag ccttgattgg gtacagaatg
    34681 agctcggtct tggttggcct tggcctcggg aactatatgg gctaatagca tactctttac
    34741 gcaatgccga tgtccaagcc tatgatattc tatttacgga gccttccact tatggtcccg
    34801 aagatgactc tcgctgtgca gaggcaatga ggtccgcagg caatgtggtc ttggcaaccc
    34861 ttacagacag aaagcctgtg ctagccgtat ccaatgcatt atacggccat gtggttgccc
    34921 gagtagattc agatggtatc tgcaggcaat accaggctgc attagataca gatgctggaa
    34981 agatcccctc tctcggatta gccgcaataa gtgctcttaa tagaaagaat tcgattgccg
    35041 acgagaaaag catctctctt tctgaggcct ttgaacctct cgagccactt gcgccttttg
    35101 aggctttcga gcagacggtg tttcttaaat tcgaggattc ttctcgcttc gagcgatata
    35161 gtgcagcgca agtccttgct ggctctccag gtaccggggg ctctgatttt gctggcttaa
    35221 atcaggagct gaactttgaa ggcaaaatcg ttttgatcgg tataaccgct ccagggctca
    35281 tggatagaca agcgacaccg ataaatcccg cacaacccgg cgtggagatt cacgccacat
    35341 ttattagcaa tgcgctttct tcatctttta tagagctttc tcccatcggc gtagaagtct
    35401 gcataactat cattgctctt gtcctggccg ctcttcttcc gaccatgcga acaaggatct
    35461 ggacaatagt gggatttgtc gcagcgatta taatcccaat cggattgagc tttgcttttt
    35521 ttcgaaatct cgtgttctac aatgcagttc ctgcgattgc agccgggctt tttgccttta
    35581 tagccgccat agttcttggt taccaagcgg agggcagaca gagagcttat ttgcgtaagg
    35641 cttttgctca gtatctatct ccggaagtga tatccgagct aatcgaacaa ccgaaggtcc
    35701 ttaggctcgg tggagaatcg aaagtgataa caacgctatt ctcggatttg gctggtttta
    35761 cagctatttc ggaaaagcta gacccagagc gacttaccca gtttatgaat gagtatctgg
    35821 ggatcattag cgaagaaata ctggcacagg gaggaactct cgacaaatat gtaggcgatg
    35881 cggtagtggc attctggaat gctccgctag acatcgaaga ccatgccttg cgcgcctgca
    35941 tggcagcatg ccacattcaa gaaaagctca gagaaatagg tcctgagctg gaaaagcgct
    36001 tcgggatatc cccgagaacc agaataggga ttgccacagg ctccgcagta gtaggcaacc
    36061 ttggctcgac tcaccgtttt gcctacacgg ccgtaggtga ttctgtcaac atagcgagtc
    36121 gacttgaaaa cctaaacaaa gtattgggaa ccgcaatact gacaataagg gagacggtag
    36181 ccgcggcttg cgcgggaagt tccagggcaa gctctccttc aagtcttgtt ccggcagcgc
    36241 aagatctgga cagcgaatta gctcccgaga tacgacttcc acagctggaa ggaaagactc
    36301 tgcttttccg aagacttggt ccggctatcg tagagggaaa actccaggcc attgagctgt
    36361 gggaattgag agcaactgag aaagacaact gtgctcaagg ctcattgatc gagccatggc
    36421 aggaagccag gcatttctca aaatagaatt tcaaagtagg aacataattg cagaaaggcc
    36481 cagcaagatc ccatcttgtt tcttgaatct cgacagtctt atgctcctat atggtgagta
    36541 gccgcgcagc tccattgcca ttgatatcct ccacgactgc aagaggccca tcgagatcaa
    36601 ttgcccgagc ataagaaagg cacgacgaaa gttctttttg gaaatagata tgccgcgcat
    36661 ttcgcctgct tctgcgactc tgcgatacat attaaaaata cgtggtaaga aaaggagtat
    36721 caaagagacg aacattcctg gatctaaaga aatgtccata gatacagggc gatagtatgt
    36781 cgattcctgg ttgagcctcg aagttggatt atctttagcc tctcttctac atgcgatttt
    36841 tctcaggctt cttaaaccgg cagtcatcca gtcgcctatt tcgcttactc gggttgtcgc
    36901 atagaaaagc ctggaataaa tgtatatcga aaacaacctg agtccataga cagctgatcc
    36961 gggcaatgtg gccaggtcaa atatccttcc gtcgcctggg aagatgcccc taacgatggt
    37021 ggcgaacaac ataaggacga ctattgaata aaaagggcct gctgcggctc tcggcagttt
    37081 taagaggggc aaaatcccta ttacaaaaag agcgagcaag aagcccacag gggcatgcat
    37141 tgcaaagctt gttgctgcaa tcaagaaaac cagcttcgca agcggatgaa acatgacgag
    37201 cttgctctct cctggttcga aagcaaatgg attagccgtg ctttcagccg ctattctatc
    37261 agccatggat aaccctcttg ataggggcag actaacccac ttcgcagaaa taatcctatt
    37321 gttgcctctc ctggcaatcc atcgaagaca attttcccgc ggtcgagaat aaggagtctc
    37381 gaggcaaggc taaatacttt ttcgatttca tgggtgagca ctatcagagt cttgccatct
    37441 gagtgaagcg taccgaagac ctctagaatc tcctgtacgg aaggataatc gaggttggcg
    37501 aaaggctcat cgagaattat gcagcgcggt tgcatggcaa gaacgcctgc tatggcaagt
    37561 cgacgcttct ctccgccgga gagactgccc gggctgcgat tctccatgcc ttcgagatgg
    37621 gcttgtgcta atgctttttt gattacggca tcgagctttg aacctttgat tcccagattt
    37681 gcgggaccaa aagccaggtc ctccccgacc gtcaaaccaa aaacctgagc ctcagtatcc
    37741 tgaaagacat agccgacctc tcttcggatc agcggaattt gcaaggcgat gggaatgccc
    37801 cggtatagta tttctcctgc cgaaggctta gaaaggccca caaaatgtct cataagcagc
    37861 gactttcctg cgccatttct tcctgcgaca agaaggaatt ctccttcctc aatcgaaagc
    37921 gagattgagt ctataccgat ccccctatcc ggaaagatgt gactcagaga gaccgcacga
    37981 aataactcag ccatttgtct gttgatcgat atggctcatg gcctttagaa ttggtggagt
    38041 caaaaccgag gcgagaatga tctttataat atcgccaggg agaaagggga taactccggt
    38101 tgcgattgct ctttcccagc caatatccaa taccgatttc agccagacta tgcctgcagc
    38161 gtatacaatt atcattccgg aaattccagc caggatattg aaaatccatt tgcctttgcc
    38221 aagttttgat ataagaccaa agaggatgac tgcgggtatg tagccgacaa ggtatccgcc
    38281 cgtaggtccg gccaatttag caaagcctcc agttcctcca gagaaaaccg gcaagcctac
    38341 cgatcccaat atcaaataga aaaccaccga aacaagaccc caccatggtc ccgaaataaa
    38401 tccagcaagc ataatgaaca tattctgaag cacaatcggt accggcgtgc caggaatcgg
    38461 aaatgcagca tacgctccaa cagaaatcag ggctgcaaaa agacatgcca tgatggcttg
    38521 agagagtttt gcgttattgc cttccatggg gcggactatg gcctgaattc atggccttgg
    38581 tcaagtagat ttccttaagg aacctcttga catatttatg caatgggagt aaaaaatgat
    38641 catggcgcaa gtagtatttg gctataatgg caactccctc aacaataata ataatcgcat
    38701 tcttggagcg gggccggagc cgaatatata ggcgaatcga agcgcgaaga ttaaagccgg
    38761 tcccgttttt ggggccggct ttttttagcg ctacacattg cgctcatttt tccgaaactg
    38821 gaggtgcagg ggatggcggt taatttctgt ttccccggtg gtaatgggat tccggatttt
    38881 aggccagtat tccgcaatga acgtcgttcc tgcggttctg gcgctaccgc tgaattcctc
    38941 cacaaggaga aaaaccatga aatcatttgc ttctttagat gcagccggca catcgattgg
    39001 cagcatattg gaacgctttc aagcttctct tgccgataaa ggactctcat tcaatgagtt
    39061 tttatccgat gcttatgtcc aaaaatggaa aggtgaacaa cttgcccatg cattaaaggt
    39121 tcgaacagcc cttgtactgg gacttcagga attcctggtt tctagaggaa ttctgaatat
    39181 agacagggta tcgctctcgc ctgtcacaga tcctctatgc catgatgtcg agcatgtgcc
    39241 tgtaattctt taccaggata cgccttatag aaccacacat tcgatgatct actccaaaat
    39301 gctcgcttgt ctaaatccat atattgaagg aatatacata gactcgtcca atatccgtct
    39361 cgaattgccc tcctcaacct cgagacacaa atacctaatc gatttctctc agatggatat
    39421 cgaattcaag cgggcaaaaa ggcttagcga ggacgactat ttcaatcggc caaaagagac
    39481 gactcaggta ttaacaaaag agctggatag ggcgctcgac tttttcgagg atctgatcat
    39541 ctatggtgta aagaaaattc tggtgctggc tgcggagagt ttgggagcac ttggagtcaa
    39601 gctcactgtt ccatcgaagc catttccaag attctatagc gatgaaattg atatgcaggg
    39661 tgactgcagt ttggaaggca gcttggaaga gcggcttggg cacaaagcca aaacacaatt
    39721 cttctgggtg ctcgggcttt tgagagagaa ctatgatctt gtatatcctt atctgtctcg
    39781 agacgggaga agaccagaaa agaaagatat ctctccaagg caggtattca attacgattt
    39841 atgcgctgcg cctctctata gagatgaaag tcttggtgca gcatacgaga tactctctgg
    39901 tggcttgcgg gaatggatct atgaatcaat cgtttcacga cttctggata atgctgtttt
    39961 aagagaatca ccgcgttttg atcgaaatgg aaatatcgag aatatggcag cgctcgatgg
    40021 ctatggccca ttcctcagca ttgctcacat gagagatgct caaggacaag ggatctttcc
    40081 acaggtttgc ggaggagggc tcggtataga gcgaacccta tttgcgcttc tccgagggcc
    40141 attcattaag gatattgatg aggtgacttg tttcggcaag aatcccgaca agacgctgcc
    40201 ttttctgttc taaagagaac tcataaactc tgtaacccga ggagagaatt cttgaaattg
    40261 gctctaagag gataccgctc ccatggttct ttgttagaaa tcagagattc gatgcactaa
    40321 taatgccatt tgctgctctc atgcccgaag cccacgccga ggtaatccct cggcttgtgc
    40381 ctgcgccatc gcctataaag tagattcctg gcaaaacctg aaagttttcg tctataaaaa
    40441 ctgggcggtt tgcatacagt ttgatctccg gatagtacat gatggtcgaa gggtgtagaa
    40501 cgccgggcac tattgtatct agcaatttca tcgcattcca aatagcccgc atcgttttag
    40561 ctggaatgca gaggcttata tccccgggcg tacaggaggg aagtgagggg ttgaagttat
    40621 atagatcgcc ggtaaaagag ttggcaaacg accgcttgcc gagcctaaaa tcgcctatgc
    40681 gctgcattat cggtcttccg cctccaagca atactgctag atgtcccaac atctgcgcat
    40741 aatcctgtcc agaggcgaga ggctgagtaa gtgttatggt ttttaatatc gcaaagttca
    40801 caagaccatt tgctggtcgc tcactagacc aagcatggcc attgatcgag taccaatagc
    40861 cttgtttttc gtcgccgtag cgctcctgca ccacataggc tgcccgcgag ttggtacaga
    40921 aagtgcgtgt ctttttagga aagaaaaatt tgggatcgta gtaatcgcgt actattgggt
    40981 aatgttcctc tgttgtttct attcggacgc caatgtcgac cacattatcg cgaaatttaa
    41041 tgccgagttc ttccataagt ctctggagaa aagcgaagcc accgcgaccg ggtgctacta
    41101 ccagagagcc atatttcagc tcgcgcttat ctgttatgat gactctggat ccaggctcga
    41161 ctccaagcat ttcttcttta aagctaaaag ttgcgccgag ccctctgagc cttgcggtga
    41221 gatccttgat cagcttaaaa ccgccgtccg tacccaggtg tgactgccta acatcgagca
    41281 attgaactcc tatcttctcc gctcgctttc tgtaaatctc tatgttgcgt ttttccataa
    41341 tatggggctt gaggaagcct tctgtatgtt cgagatagtg tttgccagtg gcctcgtccc
    41401 aacattccaa aggaaaacct ataggccaag taaaattcat cttgcaatcg ttgcgaagcc
    41461 cgccagaaga tacctgttgc ttgtcgatga ggagaatatt gaacgatggt ctcgattcca
    41521 gaagcttaaa tgcggcacca agtccggctg ggcctgtacc gacaatcaca acatcatagc
    41581 tatccatgca ttcttctcct cggcatatta aatcatttca ttcatttctg cgcaatgcga
    41641 agacaagaac agactatatc cgtcgagatt ttgaagtgat atatgcaggg ttcaagacgg
    41701 ttattcttgc ttcaatatcg tttattaaat ctcataaatg gtgtagaata tatttgacag
    41761 aacgctatcg gcgttctacc atggataatg tcctattagt gttggaaatg gacatacagg
    41821 aaggaggtaa tcatgaagag aatcgtgaga attgggctgc tatcccttgg aatcgctgcg
    41881 ctaatcgtag cctgcggacc gggagctccc aagaccgtaa agtttggtgt gttcgaacca
    41941 ctgactggcg caaatgcggg tggtggagca cttgaagtag aaggtatcaa gctggcaaat
    42001 gagctctatc caactgtcat ggttggcgac aaagaatata agatcgagct cgtaattgcc
    42061 gataacaaat ccgataaagt cgaggcggcc aatgccgcac aaaggctcgt cgacaaggac
    42121 aaagtcaatg tcgtgcttgg atcttggggt tcttcgcttt ccatggcggc cggccccatc
    42181 gtcaagaatg caaaagtctc ggctataggt ctatcctgca ccaatccatt ggtcacaaag
    42241 ggaaatgaat attacttccg tgtttgcttt atcgacccct tccagggaac agttatggct
    42301 aactatgcgt acaagacctt gggcgccaag aaagccgtaa taattcgcga agtatcgaac
    42361 gattactctg ttgggctggc gaagttcttt gccgatgcat tcatacagct gaccggcgat
    42421 gaggatgcaa ttcttgctga attgaactac aacacgggcg acaccgactt ttctgcccag
    42481 cttaccgaga tcaagaagca caagcccgat gttatctttg caccaggtaa ctataccgaa
    42541 agcgctctta tcatcaagca aggcaaggag ctcggaataa agaccccatt cttgggtgga
    42601 gacacctggg agactccaga attcatcgat gttgggaaag aagctgtaga agatgttgtg
    42661 ttctctactt tctttgcaac cgagacgccc atcacggaaa catccaaaat attcctcgac
    42721 gagtatcgaa agaaatataa caaagagcct gcagcagtaa ccgcgcttgg cttcgatggt
    42781 tacctggttg cacgcgatgc tcttcagcgc gccggcagcc tcgacaaaga aaaggttcgt
    42841 gctgcaatag ctgccacaaa ggattttccg ggtgcaaccg gaatgatcac tctcaacgaa
    42901 gatggcgacg ccgtaaagag tgctgttatc aaaaccatca aagatggcaa gttcacctat
    42961 atggcgacgg ttgaaccgta ctgatagcgc ataggatttt caccggaggt gggatggtgg
    43021 gcgcataaag gcgccctcct tccgatatat aataagtcaa tagaaggagc ggagagtcat
    43081 ggatgaatga ggttgactgc atccgctcct tttcttataa ctacgaggaa gcatgacact
    43141 ttcattattg tttcagcata tcgtcaatgc gctctcgctc ggaagccttt atgcactcct
    43201 cgccattggc tacacaatgg tgtatgggat attgaggctc attaacttcg cacatggcga
    43261 tattttcatg gttggcgctt atgttgcttt ctatgggatc acgcttttgt ctctgccatg
    43321 gtgggcagcc ctgcttttgt cgattatctt taccgcattt ttcggtatcg gcctcgagcg
    43381 agtggcctat aagccgcttc gggattcgcc gcgaatttcc atcatgataa gcgcaatagg
    43441 ggcatctttt cttctggaga gtcttgcagt tttggttttt ggaggaagac caaagggaat
    43501 gcctgttcca gatgtactct cgaaagtcat ctattttggc tctgtctcga tgattacaat
    43561 cagcttaatt attcccctct ttacctttgc gcttttgatc ttgctcttat ggattgtaaa
    43621 taaaaccaag atcggcatgg caatgcgggc cgtctctact gatatcgaag cggcgcgtct
    43681 catggcagtc gatgtaaata aggtagtctc tgtcactttt ggcatcggct ctctgcttgc
    43741 tgcttttggc ggcatcatgt ggtcattcaa atatcctcag ctaaatccgc ttatgggagt
    43801 tatgccaggc ttaaagtgtt ttattgccgc ggtcataggt ggcattggca acataacggg
    43861 tgccgtgcta ggtggatttt tgctcggtat tatcgaaata atgatggttg cattattgcc
    43921 aactctctcc gggtataggg atgcttttgc atttattctg ctcataattg tcctattagc
    43981 aaaaccgacg gggctcttag gcaagcgcca ggctgagaag gtataatcat gagtaagaaa
    44041 agttccacta gacatattct caaagatagg cacatcgaaa acggggcaat ttccaaaaac
    44101 atgctctata ctatcatttt tcttggagca tgtgctctct tcatattcct tgccgacaga
    44161 ctcttcgatg cattctcgct tcgcgtattc aatctatgtg cgatctacat tgtgctggct
    44221 ctttctctta atctcctaaa tgggtttacc gggctttttt ctcttgggca tgccggattt
    44281 atggcggtgg gagcctatgt gagcgcgctt ctcacaatgc ctgcgccgct taaagagatg
    44341 aattatttcc ttttgcctat cgtgccatgg cttcgacatg ttacgattcc attcttgccc
    44401 gcgctcataa tagctggact tgtatcggct tgcgttggct ttcttattgg tgctcctgtc
    44461 ctgcggctta gagacgatta tctagcaatc gcaacgcttg gttttggcga aataatcaga
    44521 gtcatcatta caaatgccca gtcgataact aacggagctc aggggctgaa gggtctgccc
    44581 cgcttttcta ccacctttgc ggtatgggca gcggcaggtc ttgtagtgct ttttatggtt
    44641 cttttgatgc gctcgagcta tggcagagca atgaaagcgg tgcgggacga tgagcttgcc
    44701 gcggaagcga tgggaatcaa tgtatttcgg gtaaaggtca cttcatttac cgtatcaagc
    44761 tttttagctg gtattggtgg agctctactt ggtcacatga taactactat agatccgaaa
    44821 atgttcactt ttacactgac ttacaatatt ctgctcatag tggtgcttgg aggagtcggc
    44881 tctatatcgg gttcggtgat atcggcgatc atagtaacgg tattgatgga ggcactgcga
    44941 ttcctagatg aaccgataaa cctcatattt tttgagattc cagccatgcc gggtctgcgc
    45001 atggtagtat tttctattct ccttatggtt atgattatct atcgccagag ggggctcatg
    45061 ggcaataagg aattcagctg ggatatgttc cagagtgcat ttgtcaaaaa aagcaaaaag
    45121 ggaggcagcc atgccagaac tccctgagct tatgctgagc acttcccatc tcaccatgag
    45181 atttggtggc cttgttgccg taagcgattt caatctggaa atcagaaaga acgagatcgt
    45241 tggattgata ggcccaaacg gtgccggaaa aacaacggca ttcaatgtaa taaccggcat
    45301 gctggttccg agcgaaggaa aagtcctttt taaagggaaa gatattacgg ggcttaagcc
    45361 ccacagtata acggctctcg gtatagcgcg gacctttcag aatatacgac tatttaagga
    45421 aatgtccgtg ctagagaatg tgctggtcgg ttgcgatctg agcataaata ccggaatact
    45481 agccgcaacc ctgcacttgc ctggatatcg aactcgcgag tccgaagcca ggcgcttttc
    45541 agtagagttg ctggaacaag taggattggc acatttggca aatgaaaaag gaacaagtct
    45601 tccgtatggg cagcaaagac ggctggaaat tgcgcgagcg cttgccacaa aacccaagat
    45661 tcttctcctc gatgagcctg ctgcgggtat gaatccccag gaatcgttcg agctaatgga
    45721 ttttatccgc aagattcggg acgaattcga tctaaccata tttttgatcg agcaccatat
    45781 gcaggtggtt atgggcgtgt gtgaacgcat gtatgtgctg gattatggca ttactattgc
    45841 tcaaggcaac ccatcttcca ttcagaagaa tccgaaggtc atcgaagcct atctaggggt
    45901 ggactgatgc tggaaatcaa gaatctgtcg gttttttacg gtggtatcca tgcgctccag
    45961 ggaatcgacc tcgatgttcc cgatagaaag attgttacgc ttatcggggc caacggtgct
    46021 ggaaaaagta ccacactgcg tgccattgct gggctaatca agccaagttc aggcgaggtg
    46081 atcctccagg gatcggttat atccggaagg ccaaccttag agatcgttca gaaaggcctc
    46141 gcgctggtac cggaaggcag gagaatattc tcgaatcttt cggtggaaga gaatctgctt
    46201 cttggtgcct attccagaac ggacaagaag gctgtgttgg ccgatctcga gcatgtatat
    46261 gagcttttcc caagacttaa ggagagaaga gggcagaaag gtggaacgct ctcgggcgga
    46321 gaacagcaga tgcttgcggt gggtcgaggg cttatgtcga gacccaaact gctcatgatg
    46381 gatgaacctt ctctcggcct ggctcccctc atctccaaga tgattttcga gataataaag
    46441 cagataaatt cagaaggcac gacggtattg cttgtagagc agaatgcaaa ggcagccctt
    46501 gaagtagctg actatggata tgtcttagag accggccgca tatcactttt tggtacaggg
    46561 catgagctac ttcaagacga tcgtgttcga aaggcctatc tgggcgaagc acagtaatag
    46621 agcggattct aggagctaat ccatttcgga aatatcagaa atgcctgagc cttaagacca
    46681 ttacaagcct ggcgatattg cgcaaagcac caaacaatag agctgtcata agcattgtcg
    46741 atactccagg ctcgaaggct atgatttctt ggtaagtcac ctgccatccc acaagggctc
    46801 cgatggctag gggagccagg agcactatga gaatgcgcag caccaaacca ataatggttc
    46861 tcaaaataac aaagcgggca taaagttggg atatacctgt ctcgcgatat ttgagcaggt
    46921 tcctggccca ggaataagac acgagcgttt gcaagatgat gtttagcaaa taaataatga
    46981 agatgaagcc gagtacgagc tgagcacgct gtgtagggaa aggtcgaccg ctctctcctt
    47041 cgaacaagat ttgttccgca caagagacga gctttgggag cagaaaattg gatatgagca
    47101 caccatcctg gggacagaga atcgctattg ccgcattgcg ctccggatag atgacaatac
    47161 gcgatgcata gcctcgaatc gagccccctt gaaaaatgga tcggccttct ttattctcga
    47221 tgattctcca tccaaaacca gtatcaccat ccttgtagag gggctgatag agcatgggtg
    47281 tctgcgctga agaaagccca ggaattctct ggccacgctt tggcgatacg acgaatctca
    47341 agaattttgc aagatcttca cttgtagtca ctatgccatc ggagggcgct gcatcatagg
    47401 gaactttctc gacataagga aatgtcaggc caaagaacga accggcacct acggcgatac
    47461 tcagcatcga ggagggctct gcagtagtag atctcatatc cagcggaaca gctatcttcg
    47521 attcgaggag ttctgagtat ttcatgcccg aagccttttc aagtattgtg cccacaatcc
    47581 ggtaagaaag ccgactccgc gtatatttct ccgtaggaaa atgagaactc aaaaataaat
    47641 ctgcgtctgg aagaccagcc tcgagaccca tggtttcagg atcgaattcc ggaaggtctt
    47701 tttccggaaa cgcgacacga tgtgctgcaa gaagccgcag agtaagcgga agatcgagag
    47761 aatcgtcgct cgctgtcctg gcctttagag aaccgagttc cggaatgaaa tgagtaattg
    47821 gcacatcgag ggttatcttt ttttcattta caagctgggc aagtaatgtc cccgtaaaga
    47881 tctctgaggc aggtcccata tatagaggag tcgagatatc caaaggattt cgttgcccat
    47941 caaagccata ggctttattg aagacgattt ctccaccctt aatgattacg acctcatagc
    48001 cggctattcc ctgtcgcact cgttcgtttc taaaaagcga atcgaaatct ctgtaatttt
    48061 caggcccaag gtcaggtatg tagagggcgg caagaatgac cgtagcgaca agaatccatg
    48121 gaataattcg gcgcagaaac ttcatcagtc actatcgtag caggagcgcg caaggagggc
    48181 aacatagcca tattgttcta tgtaatcctg ttcaaggctc ttgtctttcg aaccgcgatg
    48241 ctcagtagtt ttacagaaga tgcggatgct tgattcagag agtaaacagt aaatcgctat
    48301 aggtgggtag aggccaaaga tatttgggca ccagacgttc cagttcgtcg cagaattccc
    48361 tgagttcatc catgccaggc acgaccatgt ctcggtaaga ctttgctcgc tcgagttcat
    48421 cttctgtgca acttgcatca ctgagagccc tttccaggcg atcaagccct tcggccgatt
    48481 tgccaacaag ttgggctatg cgtttagcaa gagcttcttg cacgatagcc ggagctccta
    48541 tggcggcgat gcttgagcat atggaaccgt agttgctcgc tgcctcgctc gccgctggaa
    48601 tgatgtatct gcggacaagg tcgatcatca tggccgcctc gatttttatc tgcttggagt
    48661 aacgctcgag ataaatatgc tgccgagcct cgatttcttc tggcagaaga atacatagat
    48721 cttccagcat ttttacattg tccggtgtgc ttagttcctg gatagcatcg accgcggagc
    48781 gaaaaaccgg caatcctctt ttttctgcct catgcatcca gtcgatagaa tatccattgc
    48841 cattgaatac gacgcggcgg tgctcttgcc aggatctttg tgcaacaagg cgcgcggcat
    48901 cgagtaaatg tgctcctttt gtgagctctt gttcgatctc ttcggaaatg cgattcaatg
    48961 cttctgcaac cgcggcattt atcatagtga gcgaagttgc ggctgattga gaagaaccca
    49021 cggtgcgaaa ttcgaatttg ttaccggtaa aggcgaatgg gcttgtgcgg tttctgtcgg
    49081 aaaaatcacg ggggagcttt ggaagacttc ttataccaag ttccaaaaag gcattagtga
    49141 ttctctgcgc attattttgg ttttgcaggg aattgaacag gtcgtagagt ggttttccaa
    49201 ggtatatcga aagaatcgcg ggtggagcct cgtggccgcc aagtcttcgg tcattgcccg
    49261 cggtagcggt agaggaacgc aaaagccccg cgcgcctatc aacagcctct atcaccgcaa
    49321 gagaaaaaag cgcgaatcgt atgaatttga accctgcctc ttcttcggat gaaatacctg
    49381 cgacaattgc cggatctaga agattcaatc cctcatctgt tccaatagac cagttgatat
    49441 gtttgccgga accatttatg cccgcgaatg gcttttcatg gaggagtgcg gccatcccat
    49501 ggtgttccgc agtctttcgg attgtctcca taacaagctg attagcatcg gcagcgatgt
    49561 tttcctgtga gtagatacag gcgatttcga attgattggg agccacctcg ttatgctggg
    49621 tcttagcgga aatacccata gcccatagct cgctgttgag ttcgttcata aaggcagcgg
    49681 ctttttcatc tatagcgcca taatagtggt cttcgagttc ctgacctttt gcggccatag
    49741 taccaagtac ggttcgaccg cagagcttaa ggtcggggcg ctttgcgtac agctcggcat
    49801 tgacgaggaa atactcttgt tctggacctg cgtaggctat aatacgctct gttttgctat
    49861 ctccgataag gcgcaataga cgcaagccag cacgattcag agcttccatg gagcgcaaaa
    49921 gggggacttt cttgtcgagt gcgagcccgg tataggacac aaaggcagtg ggaatgcaga
    49981 gaatgtatcg ttcggcattg cgcttgagaa aggcagggct tgtcacatcc catgcggtat
    50041 agccgcgagc ttcaaaggtt gctcttaagc cgcctgacgc gaaactcgag gcatccggct
    50101 cgcctttgac aagatctttc cccgagaatt ccatgagaat gctgccatta ggctgtggtg
    50161 aaacaaagga ttcgtgcttt tctgcggtca gccccgacaa tgggtgaaac caatgcgtgt
    50221 aatgagttgc gccaaggtca gaagcccatt ctttcatcgc ggcagcaacg atttcagcaa
    50281 tatcaagcgt gagctcacgc ttgccagcct gaatttcgag tattgcctca aatgccgagc
    50341 gaggcaggcg ctctcgcata acatcatttg agaaacagcg agagccaaag tgatcgatca
    50401 atgaaaaatt cgaggaatgc tcatccataa tgagttctcc agcaaagaca agatatattt
    50461 tgaataaagt agcaagaatt gtgccaaaca gcgacacttc tgccaaaaag taatcaataa
    50521 ttccttctca tcactttctt gaatattgcc gccgggccga tacaatgaag atctttcatg
    50581 aaaagtattc tagtttcttt tttcatagac tattatgaat atcgaataga aaggcaaatt
    50641 atgctattcc gtattcaggt atgcataaaa aggcataaaa gagcactgtg tgcgaaaagg
    50701 tctccccttc tcttaaaaat tctgatatac gatttcgggt agccaaaaag ttacaaattt
    50761 gtaaaattgg ataaaggcct acaattttgt cttaaaacgc ctgggatcta tcctgtggcg
    50821 ctgaattgca tatcgcaacc ggcgctcggt gattacaagc ctccgtgccg cagcggaaac
    50881 attaccattc tccaacttta atgcttctat tatcagctcc ttttcgaccc taaagatctc
    50941 tgcatcgaga cctgttttgg gctctgtccc tgtggaagct gcagactgga gacttggggg
    51001 tagatggtag gcgtggatca caccatctgt cgaaaggatc gcagcgcgca aaatgcagtt
    51061 ttcaagctct cgtacattcc cgggccaatg ataaatcatg aggagatcca atgcaggcgt
    51121 agaaatgcgc tgaatctttt tgctgttttc ttggttgaac tgcgatacaa agtggtcggc
    51181 caagaggagt atatccgctc ctctctcgc
//
LOCUS       DAOMZL010000003        46377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_546_length_46377_cov_17.821446, whole genome shotgun sequence.
ACCESSION   DAOMZL010000003 DAOMZL010000000
VERSION     DAOMZL010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 46377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46377
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_546_length_46377_cov_17.821446"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..1049)
                     /locus_tag="PLQ30_00665"
     CDS             complement(204..1049)
                     /locus_tag="PLQ30_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPW00986.1"
                     /translation="MSSMTANVLMIGDIVGAPGLRALFTFLPTLIRKTEADLVVANGE
                     NAMKGFGISADEISAMRSYGVDIITSGNHVWERKEAVVLLESESQLLRPANYPKGLPG
                     CGVAYVGGRNHAGYPAIVRANGAAPRFEWLVMNLQGRRSMYDIDCPFAKADQLLAAAA
                     RDHPNAIIVLDFHAESSEEKEALAWYLDGRISVFAGTHTHVPTADERVLPKGTGYLTD
                     LGMTGPIDSVIGMNGDICIRRFLTQIPYKMETAEGSSALMGALFRIEAESHRCVGIER
                     IFQSL"
     gene            complement(1046..2578)
                     /gene="rny"
                     /locus_tag="PLQ30_00670"
     CDS             complement(1046..2578)
                     /gene="rny"
                     /locus_tag="PLQ30_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPW00987.1"
                     /translation="MAPLVGYIVLPLAGLILGWMIRWLYARFRLSSAEREAERVLQDA
                     IKEAEAKKKEILVEAKDQIIRERNQQERELRDRRMELQKYERRVLLKEENLDAKLASI
                     ERIEADLKNRENKIAEKEEYLAGQEERYAAELERVSGFTAEEAKKLIIQNMEQEARRD
                     AQTLINKIDQEAQLSAEKKAKDILITTIQRIATESTAESTISSVSLPSDEMKGRIIGR
                     EGRNIRTLETLTGVDIIIDDTPEAVVISCFDPIQREIAKISLERLIADGRIHPARIEE
                     MIQKVTREINQKLYDEGEKVVFDLGLHNMAPELIRAVGRLYYRTSYGQNVLMHSKEVA
                     VIAGLLAAELGLNREIAKRGALLHDIGKGIVTDSDKNHAEIGTEIAKKFGEDARVINA
                     IASHHSDVEPNCPESVIVQIADAISAARPGARRETLDNYIKRLEDLEAVAESFSGVEK
                     AFAIQAGRELRIIVSNEQVSDDQAKELCKNIAKKIESDLRYPGRIKVTIIRETRIVEY
                     AR"
     gene            3050..3346
                     /locus_tag="PLQ30_00675"
     CDS             3050..3346
                     /locus_tag="PLQ30_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPW00988.1"
                     /translation="MSKKIYVGNMNYNTSERQLQDLFAQYGDVSSVNIIVDRFTGKAK
                     GFGFVEMDNPDAASAAIAALNGQEFMGRQLRVNEAQEKPRYEREDTGGYRGRRY"
     gene            complement(3562..4638)
                     /locus_tag="PLQ30_00680"
     CDS             complement(3562..4638)
                     /locus_tag="PLQ30_00680"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPW00989.1"
                     /translation="MAIPRKQCNRKEDQKTELVSPVWEGTANLSVAIRADRFLSEIIK
                     LTTRSQLKARNARLFCNGVEVKLSHKLKNGDHLRLEWNEAISEQLEPEPIPLEIIYRD
                     ADVYVIDKPQGMVTHPAAGNWHGTLANAVLWLENNVRQDNQIKDSSSITSPTRAGIVH
                     RLDKDTSGVIIVARSASVQEFLSSQFREHQARKEYFAIVRGMPPEPKGRIDTWIARSP
                     RDRKKFAVSEPGRGKHALTLYRVCKVWQVNCNIGYRSVGVASKATKPSKYSLLALYPR
                     TGRTHQLRVHCADMGCPILGDPIYGKKDEAFPEATLMLHAHRLKIRLPSRSEPSVFSS
                     PFPTRFRDIISYLDKHGHNIKVTD"
     gene            complement(4613..5452)
                     /gene="truA"
                     /locus_tag="PLQ30_00685"
     CDS             complement(4613..5452)
                     /gene="truA"
                     /locus_tag="PLQ30_00685"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPW00990.1"
                     /translation="MAEHIHAQENPFCASKPSLRTIRLVIAYDGSDFSGWQKQKNGRS
                     VQEELEKALLIMHKHEVRVIGSARTDAGVHAMGQVAGFHTDIASIPADRFVPALNRLL
                     PRDIRVLSSEEVKPDFHARFDASLRRYRYFMSCGMAPDPFVLRYTHFSPHYPSLSILN
                     SMAFVVLGEHDFSTFASAQDKSESKCRFISESVFFLERALLVYQIAGNAFLWRQVRSL
                     VGTFLELEKKAKNPCEAEVMMREVLESKDRSKAGPTAPACGLFLWNIEYGERVHGHPA
                     KAV"
     gene            complement(5456..5833)
                     /locus_tag="PLQ30_00690"
     CDS             complement(5456..5833)
                     /locus_tag="PLQ30_00690"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPW00991.1"
                     /translation="MISGIGIDIVHVDRIRRWMNEPGILKRFFHPAEIETASLRKKGA
                     ALSLAARFAAKEAFGKALGSGLANFALKEVAVVNDDLGRPIMRLEGNARREFERHGGG
                     KIHISMTHEGDNAVAIVILEKGS"
     gene            complement(5853..6812)
                     /locus_tag="PLQ30_00695"
     CDS             complement(5853..6812)
                     /locus_tag="PLQ30_00695"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HPW00992.1"
                     /translation="MSLDSRISKLLENWPAKVISLVMAIFIMFLYNLTRLEQRVIIVP
                     LNISEGHSYVPATEYPKTVRVIIRGDRDQIYRVRESDIVASLDLARYGSEGVFRVPVK
                     LERRGDALNIDPLELRADPSEIPISFERLAAKRVAITPTFKGFLEQGYVLISYEILPS
                     EISIEGPTTLVSSIKDISTDIIELSGKTSDFSLNVPLVKPSELIRLVDTSTVKFSAKI
                     QMQERRLTLKNVPIFINNLDPSLIISDPLPSGNITLVPHPGQDETLANNASLQADFKG
                     IVKPGLYSIPLTVSAPEGFEVEAYEPLALNVRLHGAPVLPAFH"
     gene            complement(6802..7605)
                     /gene="cdaA"
                     /locus_tag="PLQ30_00700"
     CDS             complement(6802..7605)
                     /gene="cdaA"
                     /locus_tag="PLQ30_00700"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HPW00993.1"
                     /translation="MNAPTLIEFFTIYVRPVLDVGILAFLIYKTYQLLVMTDAVYLIR
                     GLLILLAIYGISFFLKLSTVTWIMNILAPGLVIGLAIILQPELRRIFIQIGRQSIFGK
                     KSMARSSWIEAAVSASVYLAEKRRGVIIVMARKVGLASYIEKGIPLDAIISTSLLISV
                     FEHDNPLHDGAAIISQGRLAAAGCFLPLSDQQEIRKTFGSRHRAALGIAEESDAVVLV
                     VSEETGALSLAYDSKLYYDLSSEEILERLHILFSEQNLRREGNEVANEP"
     gene            complement(7602..8486)
                     /gene="folP"
                     /locus_tag="PLQ30_00705"
     CDS             complement(7602..8486)
                     /gene="folP"
                     /locus_tag="PLQ30_00705"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPW00994.1"
                     /translation="MRISANRTQLKHDVNRLRLPNGRSLELGRAQEHLACIMGIVNIT
                     QDSFFEGSRRPDSIAAIETALDMVKQGASIIDFGAESTRPGSTEIDSEEELSRILPVL
                     NGFRALSDAVVSVDTRHPEVAEAALAAGADIINDIEALQRPGMIETVSKAKAAVILMH
                     MQGTPQSMQKDPYYKDCLQEVRSFLEDRSHRAIEAGISPDRIILDPGIGFGKLKEHNI
                     SLLKGIEHIASLGYPVLIGLSRKKLIGDLTGRDVASRLAGSLGAALAAWLNGAQILRV
                     HDVAETVDALRAFHEVIQ"
     gene            complement(8476..10434)
                     /gene="dxs"
                     /locus_tag="PLQ30_00710"
     CDS             complement(8476..10434)
                     /gene="dxs"
                     /locus_tag="PLQ30_00710"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPW00995.1"
                     /translation="MSLLNQISGPADLQGLSMADLRCLSREIRLSIIETVQRNGGHLA
                     SNLGVVELTIALHRVFQSPYDAIVWDVGHQCYSHKILTGRAARFVTLRRNGGLSGFPK
                     RKESKHDIFDTGHSSTAISSALGLLQARLSMGHAGRVIAVVGDGALTAGLSMEGLSNA
                     GQLSLPLIIVLNDNRMSISNSVGSLSRYLSKLSASVQYQSLRSRVDTMVLKIPRIGGA
                     IFSFINRTKRAIKAIFFKENLFVDYGFEYVGPIDGHNLVSLINAFEHIRNLNRPVVVH
                     VVTKKGKGLEKAEEDPESFHGLVPAYPDIPGPGTTSHGESFTSCFANALIACAEKEPR
                     MVAITAAMSSGTGVSKLAEHYPHRVYDVGIAEQHAVAFAAGLAQGGLRPVVAVYSTFM
                     QRAVDQVFQEVALAKLPVLFALDRAGAVGEDGETHQGIYDIVLFKAMPNLVFFAPADG
                     EELLAFLDLALTLDMPCMMRYPKAFSPCPDLPIAELEIGRGVFMREHEDARVLILAVG
                     PLSYIATDVSDELAIKGIFADVYNVRFISPIDEEYLVKICSRYEGVLTIEDGVLTGGL
                     GESVAGILMRRGIKIKISSLGFGSVPPPQATREELLSGASLDAKGIQKALHELLDFLD
                     ISKQQIAAGIPTDLSGTLSTSSEIQHAH"
     gene            10661..11143
                     /locus_tag="PLQ30_00715"
     CDS             10661..11143
                     /locus_tag="PLQ30_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW00996.1"
                     /translation="MEIELRILHLCRNIDYVHIDEPLIIAAKALSDILCCQSELLLEF
                     EEEGLSTMTEDGPKVFDTLEPSAVYRLAESKYIEQTSLNNLDLGRTSPFSLGIGEYIF
                     CQQDMDCTEGEWRLKSLLEEAIREVWWQRVECEGSWFLRLVPEDDRVAVQALRRMKAK
                     "
     gene            complement(11146..12951)
                     /locus_tag="PLQ30_00720"
     CDS             complement(11146..12951)
                     /locus_tag="PLQ30_00720"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPW00997.1"
                     /translation="MITIDELRRKYIEFFKARGHAEISGKSLIPENDPTVLFTTAGMH
                     PLVPYLLGEPHPAGKRLTDYQKCIRTGDIDMVGDSSHLTFFEMLGNWSLGDYFKNEAI
                     HWSYEFLTSPEYLGIDPAKISVTVFAGDESVPRDDRSAEIWKSVGIPEERIFFLPRED
                     NWWGPAGEIGPCGPDTEIFIDTDAPVCGPDCRPGCHCGKYFEIWNNVFMQYNKKADGS
                     YELLAHPCVDTGMGIERTVAMLQGKKSVYEIEAFTPILKVIESITGKTYGASVGDPEI
                     DRGFRIIADHIRAATFILGDPKAVLPSNIGAGYVLRRLIRRAVRHGRKLGIDGLFLSK
                     PAQAVIDIYKGPYPELLENASRIYEELEREEKKFLETLQKGEHEFEKILPNLLKNPDR
                     IMPGRLAFKLYDTYGFPIELTEELAAEHELRVDRLGFEEAFKKHQELSRVGSEQIFKG
                     GLADNSEIVTRYHSATHLLHKALRLVLGNHVAQKGSNITAERLRFDFSHPAPMTPEQI
                     AAVERIVNEQIARDLPVTMEMMPPEEAKSVGAIALFGEKYEPIVKVYTMGDFSKEVCG
                     GPHVSHTGEIGKIKIIKEQSSSAGVRRIYAVLEQT"
     gene            13086..14129
                     /locus_tag="PLQ30_00725"
     CDS             13086..14129
                     /locus_tag="PLQ30_00725"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW00998.1"
                     /translation="MRILVTGGFGNVGTSAVGALLAKGHEVLIFEQPSARSKKQKRTR
                     ELLKNAHHRLTIFYGDITDKKSLQDAIYSVGVGGIDAIIHLAAIIPPLSNQKPLLAEE
                     INVGGTRNIIEICKSLAVPPRIVFASSVSIYGDRLANPWISATDPLDPNDNYSITKNK
                     SENLLRESGLPWVILRLSYVVSSDWLPFDPMLFDVPPSTHFEVVHTEDAGRAFAMACS
                     LSNIEGRTFNIGGGPACRTTYRAYLDRLMRAFGLGNSSFLCDEIFARGDFHCGWFTDS
                     EEAEMLLHFRSKSLEDYYSEVEWRTRFIRPLGSIAAMAVKPWIRSLSPYRAKADRAHS
                     PLIVSGKPEYRQH"
     gene            complement(14050..14670)
                     /locus_tag="PLQ30_00730"
     CDS             complement(14050..14670)
                     /locus_tag="PLQ30_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPW00999.1"
                     /translation="MARVKTKEKRTLILETAKRLFATKGESVSMNGIAKEIGISVGSI
                     YTYFPSKQSLIETIIEEGWDEYRVWVEDRMVSIMESGKQDDGIGQSLCALSFLVDEAL
                     PKLFSDVELIMILLAGASSNARLEKKLQYLSSMISKLVEDSSVSLEVPLDAKFYRIGI
                     MVFLLGALETIRITSKTDIDINAADIRAFLRQLMENALGQPLPDRD"
     gene            15065..15607
                     /locus_tag="PLQ30_00735"
     CDS             15065..15607
                     /locus_tag="PLQ30_00735"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPW01000.1"
                     /translation="MFSINIGNINNYDDPILKLYTNPKTKNEVLDFFSAITNSERVAR
                     AILDNAVAHNMRPSLAFAVAYVESRYNPRARSTNRTGTLNSGLFQLNSSVYGKISEKS
                     IYDPYQNARLGISHLQEYINLNGDEFSALAAYNAGITRISEKGIPGVTFRYISEVSIM
                     EHKIMNFFIASIALSESMKL"
     gene            complement(15604..16236)
                     /locus_tag="PLQ30_00740"
     CDS             complement(15604..16236)
                     /locus_tag="PLQ30_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPW01001.1"
                     /translation="MSSQVAHEKRKQEILEKALDVFVEEGYADTTFQKIAERCGITRT
                     ILYLYFDNKKQIFNESIKRFLSSLEAEIALIARDQYLTNGEKITKISEILVEACERES
                     KMLSVVMDYLLRLKASGGNPDEKVRRRTVRMRHILADIVIEGIKCGEFKESSSVKGTV
                     EMFFALVEAAVFRLVVLGRKEAAGLQEALTPFVDNMLNPNFERNIENKED"
     gene            16366..19878
                     /locus_tag="PLQ30_00745"
     CDS             16366..19878
                     /locus_tag="PLQ30_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPW01002.1"
                     /translation="MALQILREFRSQNIPPKHLWMPSGAISLPDAVSARFVAQKYKLS
                     AGELRALSLIGEAFRIIIDLYRKQYSKLLEEIALKAFASTEDNKALWEVLQELITEFP
                     PAPIYDGLAEPKDWLKSLSPVGDDSSKPNLELAIEQLILVRLFNENPAFWPYRSLFDD
                     GVSPAGATLPDSISAKTPYLQVFARLEDALKTLPGLSYGGGKTLDLINFLREPSRHAP
                     ASLKDQLEWIIKNWGTLLGDFKLSLLAGIDMINEETRPHFPPGPGLAVPYQYRSSFHE
                     YEKFSPDKNWMPSLVLIAKNALVWLHQLSRTYSREISRLDQIPEEELIIMAERGINGL
                     WLIGIWQRSPASEKIKKLCGNSEAAASAYSLFDYEISPELGGWEALDRLREQCGQYGI
                     RLAADMVPNHTGIDSLWIRTKPELFMSLPYCPFPSYSFNGPDLSGDPSIGIWLEDHYY
                     NRGDAAVVFKRLDRHTGEVRYIYHGNDGTGMPWNDTAQIDFLNPASREAVKERILSVA
                     AHFNIIRFDAAMVLAKQHIRRLWYPAPGSGGAIPSRSDHAMSEEAFDKAMPNEFWREV
                     VDLCAEKASDTLLLAEAFWLMEGYFVRTLGMHRVYNSAFMNMLKDEKNSLYRLTIKNT
                     QEFDRDILKRFVNFMSNPDEETAVAQFGKGDKYFGVATMLATMPGLPMIAHGQIEGFT
                     EKYGMEYKRSYWDETPDRDLIARHEREIFPLLRMRRLFSEVENFYLFDYMQDDGTIDE
                     NVFAYCNGQGERRVLVFYNNHWERTLGRIHTSCAFARKTADGKKQLKTTSLANALKID
                     SSPKNYVIMHEIRSGLWYIFRSEDIASRGFKLALEGYQNKVFIDIMNVHDTEGRYTKL
                     FEIVDSRGIADLDDALLEADQPELYRSLHNAINSLSSIETIQNLSQEEAIQRATIFSE
                     IFFSRLCEIAGSDDTLAASRSDSVRSASSWLKTVLKLLYGTTESDAGIAAATLCNNSS
                     ASNSEYQQYRLILLIYSFMRSLVKAFAADELNEEVSRVVKEYRIAKKLTESAVGLSRR
                     NDSHDTKYHVSICAEIAIAWALRQDKLFFDTRRTIDSTLTPNKRAQEICAWAFSDPLM
                     REALNINQYRGTEYFNKERFEAFASLLPAFAWIDSIQEETKEREWKEDPSWKEVAEIL
                     KENAIVAGYRTGIMLELMASVAQT"
     gene            19925..22795
                     /locus_tag="PLQ30_00750"
     CDS             19925..22795
                     /locus_tag="PLQ30_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPW01003.1"
                     /translation="MPKSVDSTKIAEGYKSLLGTPLGRDISQHLQDENIVFVFPSQDT
                     ADSWARAVVYSEISRAVALNRFLGFDNFLLYVAKANEQDDLSVLKPIHRWIWAMQILS
                     KEQKGERKPGKRLSTLLPSMEISTKNLTRLVRLAPYLFEIEMLTKDSAVLDASPCRFI
                     KEEFEEMRLLAEDYIDFLDRARLRDSHRRPLVLPPFMKVRAYGLRDDFIRFGLIDSEE
                     LGDGNINFRGILGEETGHEKSLSAKERIAFPDIFTQERGEKAPMLYHQFNSSLVEIEW
                     ILEEISNEIEQGIEPEDIAISVCGLTAKKEAWIRQIAAEYGVPVSIRRGKPLSMSPFG
                     RVLMAIQNAAREGLTLESLDAFADLANITGRDPEAWKALRDTALSLRLPSPSPSAAYI
                     HSLWKGAFGTDQCPESTAQIYQRLWESILSIARADSFSRLYDQIIGFLEHWIDTSGFG
                     ANIQTDRSMRLTLDELQSWIDHENIFTDLSIPPFELFLTVLESKSYIPLVEENAVYIY
                     DFDTTAGIAVLSHYVAGASQEGLASSLKIKSALPPELAGILQQEKTDKAHEVINMHRV
                     SPTQFSFAAKGFEDYETAYPLFPLPIMIDPNAERGLRAILISKEQKSATESYSHPEPI
                     EPTVARSFLNATKSFDSEKKYAIFSPSSLKELSQCGFRWFASKIDLVDVYSRDDSAIA
                     IGNLYHSAYRRAIQALPQNFKNAETDEEFRKTINSALSKEAKRILLEYGNGLQPILSA
                     LFPRAIHRLNELWNFERTMFSAYEREGFEMRLQHEFSEEEAILEGRIDCLFSRQDESL
                     GNIKCYVIVDYKKNRIPSISKMRVGVMPSDYEEFASYDENQEKEDGYDSSAFFLEEIQ
                     VPSYTLLVETSWGRVEGAFYWSIEKAEAVGYIRPPTVPKMRSAYAYSKETDFQRSALR
                     DMLRVASSKVKAGNFLDLALDRAVCEDCEFKPLCRFWYFLEL"
     gene            22795..26517
                     /locus_tag="PLQ30_00755"
     CDS             22795..26517
                     /locus_tag="PLQ30_00755"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPW01004.1"
                     /translation="MISHHDSFDLEALKLDDFQKKAAYTEDTAIVTAGAGTGKTRALV
                     GRFLYLVLQKNVDPERILALTFTRKAAAEMFQRVQQALSPFANDRPNLARSLMQTHIQ
                     TLDSFCHEIVAQAAVLYGYTPDFQIDESKCARIAEKTAYRFVLDNQKKDGIESLVFAF
                     GFQAVVQDLFASFGRANVEPHWQGKQICHASARIGEQEFKKAAEEIAEKIHDIALNIL
                     EIANQKPGNYAIHGFREGTNQAIRAARTVSCLSYDLKGLKDFSDYMEKPENAINLQSV
                     GRNETESAIKELAKELKNKALRDRISQLVDFEKFLPSYYAIMDRLDEYADMLCEEKCI
                     ANIMNYKDLGALAVDILSNHEDRRIFWASRFQYILIDEFQDNNELQKQLLFLLSKEGK
                     EIRRGKLFFVGDEKQSIYLFRGADVSVFKGLANELMAAPLSLNLNYRSTNQLIEFFNS
                     VFRIVMLPANPENPQPFEALYQDMETISTENANIEESKGFSSRIEYHCITNKIPAEEE
                     ACGIRSAKESMAFRLALWIRDAVESSNPLLIRKEGETQDLAITEKKQHKLSLRKAEYS
                     DIAVLMRTTRRQYELEKYLRMLAIPFVSDTAASLFSEEPINDLYYILRLLIDAKDLFA
                     TAAVLRSPLCRISNDGFVSILTEKKSLADLEADMPIKLNPNDRDSIERMVLFYQELRQ
                     KSDHMPLMELVAYIWKEGRLEASILCDEQRLPYLDHWNAIRTLAANTEAEGGHISAFL
                     ISIRDYMQNMHLFDTNSVPKAEAHGVHLLTIHKSKGLEFPIVIIPWTEASTNKNGGKE
                     LWGILESAQEKGRTRFYTVDIGFHDRIQSGSNILQTQAKAQRTEKEQAETKRLLYVAC
                     TRAIDHLIFFSAAPENKPYDRDSFHALLFQSTAHLIPRDYSEKTQLPAHLSGLQLFFE
                     PLVSDKEIQGARSNVLRARKLSQKKLLSGKEKQESEQKSKEASCKTAPKTEKSFIEPA
                     KMTNLPSDSVAGSHSRRITASSINAIIAQQSLAADYARELSGHFVSKRIFSPAESRAQ
                     SEAQALDETSLEAQNTQESLDFSAYGSLVHELFVHILAEKTMENFRPSASLSRELLGS
                     TSPEFGDTSIPKKTDPRYLSSIIENAAAELAPLFEAPTFSTLVLEKKYSCEFPFLLGL
                     GRWIIEGRMDFVAQSDEEVIILDLKTDRRFLPHKYALQLGLYCIAAQQLFQKKKVHAG
                     LIYLHFGQIAWLNANPDIEILLKICDSICFDTHVNS"
     gene            26531..27115
                     /locus_tag="PLQ30_00760"
     CDS             26531..27115
                     /locus_tag="PLQ30_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPW01005.1"
                     /translation="MKIPSRKNGAKKTTKKTEPTPLRIFLTLAQINTLTLGGGYVIVP
                     VIGNSFKKQGWIDEEEFYRIFSRAQVFPGPIALSTSFLCSYKIAGVKGAIAAVLGVVL
                     PPFIALILVGGFISLYGDTELFKRFLKGAGAVVPGLVGSMLWKTAKNRKWTTRALIEV
                     GLLTILLVLFPSAAFFILIGGIVVIYLGRLIWKS"
     gene            27103..27615
                     /locus_tag="PLQ30_00765"
     CDS             27103..27615
                     /locus_tag="PLQ30_00765"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPW01006.1"
                     /translation="MEILKISLAFFKIGIVAFGGGWSVVGLIKQEIVPSWMSVDAFSS
                     LIAIAQSTPGPIALNAATLVGWQKAGFFGALAATFSVIAFPLLAMSLTLAFSRYIPIK
                     KDLADESLRSGSMAMIIMTLWTLLPQNTIPMPWIFALASFGITAFTQINGVWVILGSG
                     AINMLLGLLP"
     gene            complement(27628..28581)
                     /locus_tag="PLQ30_00770"
     CDS             complement(27628..28581)
                     /locus_tag="PLQ30_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HPW01007.1"
                     /translation="MTIPKYPEFAPLTLEARDILYPALNLLPDGISEFTFSGLYLFRN
                     TYGYEISQISDEKFIISGRYKGRTFFYIPCCFPSMEQFDELMLNFDYMRHLSECQAAK
                     QRIELERRGYLVREDRDNFDYLYNREDLATLNGRVYHKKRNLVNGFVSNYACEQRPLT
                     KDRAADALQVLEEWRAAKGADGDYAAAREAIECFNILGLRGAVYYIDRNPVGWCLGEP
                     LAKGKMFAIHFEKAIDSYKGIYQFINQAFAQSLPKHYLLINREQDLGDLGLRQAKMTY
                     RPVGFVKKYTVERSGLANIVPAPIEPPAECGPGTVHACDED"
     gene            28740..29996
                     /gene="pepT"
                     /locus_tag="PLQ30_00775"
     CDS             28740..29996
                     /gene="pepT"
                     /locus_tag="PLQ30_00775"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HPW01008.1"
                     /translation="MIAILDYLNRSFTEEMLRRFLSYVRIDTQSNRHAEKTPTTEGQW
                     DLARQLEQELRELGISELELDRHCYLIAHLPASPGKEHVPCIGLMAHMDTASDVSGSG
                     VKPRVIKNYDGKGIWLSEQYLLDPGEFPDLYEHVGDTIVVTDGSTLLGADDKAGIAEI
                     MTAVAYLLQHPELKHGPIDIYFTPDEETGKGMDIFPLGKARAKACYTLDGGAAPEIEA
                     ECFNAYAVKADFFGKVIHIGAARGRLANAVAMAASFIDMLPRSESPEATDGWFGYYCP
                     IEVSGGLEHAWTEVYLRDFSASRMQERIAALKAFAAAVEAQFPQGHIELNVTQQYLNM
                     KEKLDKHPEVLERLKNAIKVLNIEPIMKPIRGGTDGSRLTEMGIPTPNLFTGGYNYHS
                     RFEWASLSEMALSVDTMLNLVQGWAD"
     gene            30264..30815
                     /locus_tag="PLQ30_00780"
     CDS             30264..30815
                     /locus_tag="PLQ30_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01009.1"
                     /translation="MFSSLDFKEYEQVAGNSGAFKGVEIDLMLEILKDWQQAPGKNYT
                     LLELRDGKTLVAFAIIHKAAGRNFTFDIRFVVLDSDYRSTNAIQHLFEMIDQELLKKM
                     AFAVIRFETSTRKRDSLGENTLENSGYSQIGHIVDFYGANEDFFFYIKAIFRNPPNFI
                     KISKPFEEDLCSECLPEEIDNQK"
     gene            30826..31764
                     /locus_tag="PLQ30_00785"
     CDS             30826..31764
                     /locus_tag="PLQ30_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPW01010.1"
                     /translation="MIVGAIEGGGTKFVCAVLQFDDPNIPEKTPRILRIERIPTTDPE
                     PTLDAVVRFFKDSEIECVGLGMFGPIECRIESPLWGYLLKTPKPGWSDIDIAQRLQRK
                     LGVPIKLETDVAAAGLGEWKWGAGLGTHCCVYITIGTGIGAGIIIDGIPMRASFHPEI
                     GHMRVPRARTADSGPDEFEGLCIHHGDCLEGLASGPAIAARWGEAPENMSIEHPAWKL
                     EAEYLAYALTNIALTVAPDRIIVGGGIGLREGLLDLVSERMRELLGGYIQALNSTAAI
                     RLFLARAELGSKAGVLGAAELARQTINSVSSVSNRL"
     gene            31891..31963
                     /locus_tag="PLQ30_00790"
     tRNA            31891..31963
                     /locus_tag="PLQ30_00790"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31924..31926,aa:Thr,seq:cgt)
     gene            31977..33248
                     /locus_tag="PLQ30_00795"
     CDS             31977..33248
                     /locus_tag="PLQ30_00795"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HPW01011.1"
                     /translation="MNSIVHRFGRVVSNQVLENPQRARSLLCTVYALSSWKDAYFRGR
                     GLRGIYARMNSVISKNMSRSFRDSSHSAMVNIFFPCELLYAMEIVPVFPEALSVYIAN
                     SGCGEIFAESAEAIGISESLCSYHKIMIGTAWTGVLPKPLLIANTTLACDANQLSFRL
                     LADEYHTPHIIIDVPHTVDKESVHYVSSQLKNITSLLEDLSHCPMAESKLQDTIDRAN
                     RTIKAYRKYLDLRAEISLPISMTGELFSLAALHAFLGMPESEQYAIALEELAHKASLD
                     RRKKRILWLHTLPNWQHSIRVLLENNEHFEILASDMTYDTLIDMDPKKPYESMAKRLV
                     YNINNGGAHRRIDAAISYAEKLHADGVIVFGQWGCKQTMGMSQLAKISLEAHGFPTLV
                     LDGDGCDPRNVADGQMLTRISAFLEQLEAKR"
     gene            33245..34975
                     /locus_tag="PLQ30_00800"
     CDS             33245..34975
                     /locus_tag="PLQ30_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPW01012.1"
                     /translation="MIGYTCKYTPIELLYALGETTELLNHEVPDFEYAEKWTHPNLCC
                     HAKAFLQHSSELDELVLVNCCDSIRRAYDVLVAQQVHRFLFLFDLPHIDKGCAQEKIK
                     KELFRLTRLYTEHHPVSFDRQRFLEACRASSEVAFKASFESASGTHSKLDPATPFIAI
                     IGARVSQELLSTIKEEVPYLVIDATCGGNRYLSPLAPGAERMSFEDLMDWYAKELLHF
                     IPCMRMANIEPRKALWENENLCGIIYNTVKFCDYYSFDYARVKREVRLPILKIESDFT
                     PQSEGQLSTRLEAFQENLHLSLEKPLKIKAKQVSQPAFFGGIDSGSTTTKMVIIDRNS
                     KILASSIVSTGAKTQVSARKVLQETCRQLGVEPCDLASVFSTGYGRNNIAFATDSKTE
                     ITCHAKGAFFLDPTIRTIVDIGGQDSKIIRIDNEGRVVNFVMNDKCAAGTGRFLEMMA
                     RTLELDIDIMVRTGLAWKNDLTISSMCTVFAESEVVSLIADNCSIADIVHGLNKSIAS
                     RITSMISRTGCQAPYMMTGGVARNTGVVRELEVKLGERLIVSEKPELCGALGAALSAL
                     ELSALGSKEI"
     gene            35025..35885
                     /gene="mtnP"
                     /locus_tag="PLQ30_00805"
     CDS             35025..35885
                     /gene="mtnP"
                     /locus_tag="PLQ30_00805"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HPW01013.1"
                     /translation="MEKAKVGIIGGSGLYEIEGAEVVEEIKVDTPWGSPSDLITIAKI
                     GGVRTAFLPRHGKGHFLLPTEVNSRANIAALKMLGVREIVAFSAVGSLKEDIHPRDFV
                     LPSQIIDRTKDRPGSSFFGEGIVAHLSFADPFCTRLCSIIEPLILARGLRLHTDETLI
                     CMEGPAFSTRAESQLYRSWGAGIINMSVLPEAKLAREAGICYAMICMATDYDCWKMDH
                     ADVTVDMVVQNLNANSAHAKALILDVVPALSIDTECSCHTAMKGGIMTAPSKRNPETV
                     IKLKTIFPEL"
     gene            35954..36385
                     /gene="rpiB"
                     /locus_tag="PLQ30_00810"
     CDS             35954..36385
                     /gene="rpiB"
                     /locus_tag="PLQ30_00810"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPW01014.1"
                     /translation="MKILIANDHGAVALKFNILRWLETQGHVVVNLGVDTEDRVDYPD
                     QAALAVTEYFKGSYDLGIVCCGTGIGISIAANRFKGIRCALIHDSYTARMAKEHNNAN
                     FLAFGGRVDYKEPVEELLSVFLHAEFRKGRHAERVAKLDTI"
     gene            complement(36393..37814)
                     /locus_tag="PLQ30_00815"
     CDS             complement(36393..37814)
                     /locus_tag="PLQ30_00815"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW01015.1"
                     /translation="MSINIRSLGAAEEVTGSKHLLEVDSTRILIDCGAFQGKRAKADA
                     KNRALLGDDIEASSIDALVLTHAHYDHCGLVPYLVKRGFTGNIYATSATRDLANLIMT
                     DSAHIQARDADYLSRQAEKRHEQFDWKPLYDNFDVIRAMDQFVTVSYHRPISIADGVQ
                     IEFFDAGHILGSAFARITAKDKAGNVAILGFSGDLGRKGKPIIKDPECLNGIDFLILD
                     STYGDRLHESTADSLLHLEDVVNRTAKKRGKIIIPAFAVERTQELIFSLHLLHDQGRI
                     PDIPIWVDSPMAFNATSIFVIHPECYDKETYDLFTAHAKNPFGFAALNFSQSVEDSKA
                     LNQAKGPLIIISADGMCEFGRIQHHLMHGLGDSANTVLIVGYMAEGTLGRRLRDGAKE
                     VRIQRDWYQVRANIEEIDAFSAHADWKETIEWLECIDKKRLQRTFLVHGEQEALMAMQ
                     GHLIDADLKDIEIVKLGVFYKIA"
     gene            37872..39044
                     /locus_tag="PLQ30_00820"
     CDS             37872..39044
                     /locus_tag="PLQ30_00820"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HPW01016.1"
                     /translation="MIADTLTLRVEKLSSNGEGIAFSEGKAIFLPYTIPGETVLAEII
                     EEHSGFARARIIDLKESSPYRVEPPCHHFGDCGGCSLQHVGYEHQALLKKQAALDTFL
                     RIGGFDPGELQIIIGEGYHCRNRAQIHACGDGGLGFAKTGSHESVRTSHCPALVPIIE
                     RWLVSENRKAHPYRALSALIGDRPRFVVFGQENRIYLEGRDNFAKAKVAGKELRFPLA
                     GFFQSNIFMLERLIAENVISLYGKRALDLYSGAGMFSLFLADRFEAIDCVESEAASIE
                     AARMNLESAAVKIEFSCTTVENWIEAARSSLSFDCILADPPRTGMTPKVRTWLSFVEA
                     PMLLYISCDLASLARDLRDLFQKGWQLENIILYDFYPQTGRLEAAARLIKEAKHGP"
     gene            39034..40614
                     /gene="pyrB"
                     /locus_tag="PLQ30_00825"
     CDS             39034..40614
                     /gene="pyrB"
                     /locus_tag="PLQ30_00825"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HPW01017.1"
                     /translation="MARNGPFKGRSISVVNDLSLDEQLYLYRKARELKETARGGHDAE
                     KFRINDLDYQVYLIFMENSTRTRESFRNAGKFLGARVNVFDAATSSFNKNESITDAIK
                     MLFGYSNESCFILRTPLEGVCTWLDQAFEEYAHITEKLKPSFINAGDGKHEHPTQEFL
                     DEFSFLEQLNWKEEHIHIAMVGDLYHGRTVHSKADGLRIFKEVEVDLVAPSLLSMPSY
                     YVDKMRASGFQVNIFESIDEYLAQDNIAPIWYFTRLQLERMGEAVLERAPYLRQSVTF
                     KKDYLGRLPENCRFYHPLPRDRNYPTIPFFLDDLPLNGWDGQSINGYWTRITEIAMLS
                     GRIGGDFEGEHAKPREFIDDFVHEASIQETSKPEYKVGIKPVEEGIVIDHIARGASIG
                     EIWDTIDAVRKILKLDVRSSHGVYHSNRGEGIYKGIISLPDIISFGEKDLKKLAAIAP
                     GCTLNLIRHAHVAKKYRLSMPPRIYNFDEISCKNENCISSPAQHEGVPTEFIRKGETT
                     FVCKYCEREHKFRDIWDI"
     gene            complement(40726..41469)
                     /gene="rlmB"
                     /locus_tag="PLQ30_00830"
     CDS             complement(40726..41469)
                     /gene="rlmB"
                     /locus_tag="PLQ30_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPW01018.1"
                     /translation="MEYLTSFHAILETLKAGNSQCCLYITENSQRRGPRIREILDEAL
                     SQNISIQYVPESRLDEMSKDQRGAILALEEVFKKKPSLKALCDRVSEPRPSLILVLDH
                     IEDPQNLGAILRSADAFGVDAVIMPQRRASPISNAAARASAGAIAWVPILRLSNLRSA
                     VDTLKKAGYWVYAADMDGKPLTSAGIAPKSVIILGNEGSGVSQILRKVADDIVSIPMQ
                     GHVESLNVSVSAAILMYEFWRSNSGASSI"
     gene            complement(41521..42342)
                     /locus_tag="PLQ30_00835"
     CDS             complement(41521..42342)
                     /locus_tag="PLQ30_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HPW01019.1"
                     /translation="MAVIAISRELASYGEETAQELSKINGYKIVDKEHIEAALSAMGI
                     DVEKQARYDEKNPGFWASLSQQRDEYLHFLTQTVYDTALENNCIFIGRGAHIILRGIQ
                     NLISIRIGASKTVRIERVRKAQNIDSRHALQIIESSDHDRAGFHKYFFSVDWYNPSEY
                     DMTLTTDRFDPIHAASAIDAFRVSFITEDREKEAQAKLRDLLLGAKIVTEITYNRKIP
                     IHFLEAAVERGVVVLHGVANSRATVESAVAAANLVPGVKQIESAIQLVQEFTVIP"
     gene            42489..43388
                     /locus_tag="PLQ30_00840"
     CDS             42489..43388
                     /locus_tag="PLQ30_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPW01020.1"
                     /translation="MERFALLAAFGTAVCWAFSAIFFETASRKVGALAVNFWKVVFAF
                     LFLGISGMVERNVPFPYDAPLRTWILLGLSGIVGFVISDFFLFNAYILIGSRITVIFQ
                     SLTPIFTALFAFIFIGERMQVYRLIGMAVTVAGILIVVTTRSRQNKKNNKEKLSAKGL
                     LYGFLSSIFQAGGMVLSKAGLGNYSPISGTQIRAFVAIFGFAINALLIGQGRFVFVEM
                     PRIKEALRSTLKGSIFGPFLGVALSLFSMQHTQVGATSTLMALTPVVIIVPSVLILKQ
                     KVHYLEVLGAAVAVVGAALFFLL"
     gene            43601..44626
                     /gene="tdh"
                     /locus_tag="PLQ30_00845"
     CDS             43601..44626
                     /gene="tdh"
                     /locus_tag="PLQ30_00845"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HPW01021.1"
                     /translation="MKALVKRNAEPGLWMEEVPIPNIGDNDLLIKVKKTAICGTDVHI
                     WKWNTWAQRTIHIGQIVGHEFVGEIVDKGRSVQDYKIGERVSAEGHIWCGTCRACRAG
                     KHHLCPNTIGIGVNRDGCFAEYVAVPAQNAWHVHEAIQDDIAAIFDPFGNATHTALSF
                     DMTGEDVLITGAGPIGCMAAAIARHVGARNVVVTDVNPWRLELAKKLGATRVVNVDKE
                     DLKEVQKSLGMVGGFDIGMEMSGNPKALEQMIENMYNGGRMALLGLLPDGAGIEWSRV
                     IFKGLFIKGVYGREIFETWYKMQTMLLSGLDISPVITHRFRFDDFEKGFEAMLSGESG
                     KVILNLE"
     gene            44657..46135
                     /locus_tag="PLQ30_00850"
     CDS             44657..46135
                     /locus_tag="PLQ30_00850"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HPW01022.1"
                     /translation="MIEHTLLYNFRISEDAASALDKIPRCIEEANPISFQHRAELRYS
                     IRSLWRLLTMGRAQRASDYLNNPTLFSAYLRYFMPWNLVRLVSLLTDLPVELKPNATI
                     TDMGSGPLTLLLSLYCSKPELRKLPLTIICVDRAPRMMETGKTILEILAAEHSGELPP
                     WKIELRHLRFGEPIREKADLFCAINVFNEFFWKSKETLFENADALYRSIMQYCKNDGS
                     LLILEPGEPRCGGFLSALRAVAIRDNCEILAPCPHTSACPMPGIFKSGQEYLIKENPK
                     NIIKKGIPRFDSIESKANVARQKSEYIIKQLPDESSNHRNRFLTPVRMPAPRVKYPWC
                     HFSMPTSYAPKWLYRLSQEAGLAKDKLSFSYLYVKRCPSSILPSDQNKKQKSRYPGNL
                     PLPTGANEGYDELACRIVSEPIVLSHSRVGKYACSHLGYTLISTGQSEALPESGALLR
                     IATKIPASTSSSKKRKKEMEYDQKTGAILISYESPKKFSGRK"
BASE COUNT        12657 a        10881 c        10547 g        12292 t
ORIGIN      
        1 ctctcggcac tagcacctca agctagcgtg tctaccaatt ccaccatcgc cgcaagctga
       61 ttcgtttatt ttctcgttta tatatacctg cctgaaccca aatgtcaaga ggccttgaga
      121 aataggcatc agaatcgcca gaaacaccta ctaacaaaca cctattatga aggtaatcta
      181 tgaattcttt ccagtatcga tctctataag ctctgaaaga ttctctctat gccaacacag
      241 cgatggcttt ccgcctctat tcgaaaaagt gcgcccatta acgcagaact tccttccgcc
      301 gtctccatct tatatggtat ttgggtaaga aatcggcgta tgcatatatc cccattcatg
      361 cctataactg aatctatagg accggtcata ccaagatcgg ttagatatcc ggtgccttta
      421 ggcagcactc tttcatctgc agtaggcaca tgggtgtgag tccccgcgaa tacagagata
      481 cgtccatcca gataccaggc tagagcctct ttttcttcac tggactctgc atggaaatcc
      541 aacacaatta tagcgtttgg atgatcgcgc gcggctgctg caagtagctg atcagccttt
      601 gcaaatgggc aatctatatc gtacatgctt cgtcggcctt gtagattcat cacaagccat
      661 tcgaagcggg gagctgcacc atttgctcgt acaatggccg ggtatccggc atggttgcgc
      721 ccacctacat aagcgactcc acagccaggc aatcccttag ggtaattcgc cggacgcaat
      781 agctgtgatt cgctttcgag caaaactaca gcctcttttc tctcccagac atgattaccg
      841 ctggttatga tatcgacgcc ataggaacgc attgccgaaa tctcatccgc actgatgcca
      901 aaacccttca tcgcgttttc gccatttgca acaacgagat ccgcttcggt ctttcttatt
      961 aaggttggca aaaaagtaaa gagcgcccga aggccgggcg ctcccacgat atcaccgatc
     1021 ataagtacat tggcggtcat agagctcatc gcgcgtattc cactatcctg gtttccctga
     1081 tgatggtaac cttgattctt ccaggatagc gcaaatctga ttctattttc ttggcaatat
     1141 tcttgcagag ctccttggct tgatcatcag aaacctgttc gttgctgaca ataatgcgaa
     1201 gctctcgacc tgcctgaata gcaaaggcct tttctacacc agagaaactc tccgctacgg
     1261 cttcaaggtc ttcgagacgc tttatgtagt tatcaagagt ctcacgccga gcgcccggac
     1321 gggcagcaga aatggcatct gcaatctgca ctatgacact ttccggacag tttggttcca
     1381 catcgctatg atgagatgca atcgcattta ttacacgggc atcctcacca aatttcttgg
     1441 caatttctgt gccaatctct gcatggttct tgtcgctatc tgtaactata ccctttccga
     1501 tatcatgaag aagagcacct cgctttgcta tttcgcgatt tagaccaagc tccgccgcca
     1561 aaagtcccgc aataacagcc acttcctttg agtgcatcag tacattttga ccatagctcg
     1621 tgcgatagta gagccttcca acagctcgaa tcagttccgg agccatgttg tgcaggccaa
     1681 gatcaaagac aaccttttcg ccttcatcgt agagcttctg attgatctca cgagtcacct
     1741 tctgtatcat ttcttcaatg cgtgcagggt gtatgcggcc gtctgcaatg agcctttcaa
     1801 gcgaaatctt cgcaatctcg cgctgaattg gatcaaaaca tgatataacc acagcttctg
     1861 gtgtatcatc gattatgata tcgacgccgg tcaatgtttc tagcgtgcga atgttgcggc
     1921 cttcgcgccc tataatccga cctttcatct catcggaagg aagactcaca gaggaaattg
     1981 tagattctgc agtcgattca gttgcgatgc gttgaattgt ggtgatgaga atgtctttgg
     2041 ctttcttctc cgccgacagt tgtgcttctt gatcgatttt attgatcaag gtctgagcat
     2101 ctctgcgtgc ttcttgctcc atattttgta taataagctt tttagcttcc tcggcggtaa
     2161 agcccgatac tcgctcgagc tccgcagcgt atcgctcttc ctgtcctgct agatattctt
     2221 ctttttctgc tattttgttt tctctgtttt taagatctgc ctcaatgcgt tctatagatg
     2281 caagctttgc atcgagattt tcttccttga ggagaactcg ccgctcatat ttctgcagct
     2341 ccattctcct atctcgcagt tccctctcct gctggtttcg ttctcgaatg atctgatctt
     2401 ttgcttctac caggatctct tttttcttgg cctcagcctc ttttattgcg tcctgcaaaa
     2461 cacgctctgc ttccctctca gcggaagaga gccgaaatct ggcatagagc cagcggatca
     2521 tccatcctag aataagaccg gcgagaggaa ggacgatgta ccccactaac ggtgccataa
     2581 gccctcccta taaaataata tgccgtactt ttgattatag cgatggaggg gcctttgtca
     2641 agtttgccta tatttccata attcttggct ttttatggtt ttttggtggc tgaatatggt
     2701 caaatccctt gacgatctaa gaaaaatggt ttacattctg cagatataag tgaaaactga
     2761 ggagggacaa tcgccttcca taggttttcg ggttggaatt ggctgtttca tgcattgttt
     2821 ttcctttcgt aacactggct tgctgatggc ggcatacgca agtgccggtt tttctcttcc
     2881 ttgtttttca ttcggaaaga ccaaaaataa tcaaaacagt tctctgattt ctcctctgaa
     2941 gactttggat gctaatatgt gccttcaggc tttcgctaca gattcgccct aggcgaatca
     3001 agccggccta cgtcgagagg acgaggccta gatttgaggt ttttccgaaa tgtccaagaa
     3061 aatttatgtc ggtaacatga attacaatac ttctgagcgc cagcttcagg atttgtttgc
     3121 tcagtatggc gatgtctcat ctgtgaatat aattgtcgat agattcacag gcaaagcgaa
     3181 aggatttggc ttcgttgaga tggataatcc cgatgcagcc agcgccgcaa tcgccgcgct
     3241 caatggtcag gaattcatgg gtaggcagct ccgcgtgaat gaagcgcagg agaagccacg
     3301 ctacgaaagg gaagataccg gcggttaccg aggccgcaga tactaagaca ataaagactc
     3361 gcacctaaaa gccgctagcg cgagtctttc acaagaacca caaagccagc cttaggtcac
     3421 aaagccatat gggggctatg ttgtggtaaa tagaaccggt gcaagcataa acttgtaccg
     3481 gtttttttat aattctataa tctaaggcct tcataaggcc ttttcgatat taagaaatac
     3541 tcaaatatga cattgtgatt tttaatctgt gacttttata ttatggccat gcttgtccag
     3601 gtaagaaatg atatctctga atctagtagg aaaaggagac gaaaagaccg agggttctga
     3661 tcgagaaggc agacgaattt tgagccgatg agcatggagc ataagggtag cttcgggaaa
     3721 ggcttcatcc ttcttaccat agattggatc tcctagaatg ggacaaccca tatcggcgca
     3781 gtgtaccctt agctggtgag ttcgccctgt tcttgggtat agagcaagca gcgaatactt
     3841 agaaggcttt gtcgctttcg aagcaacacc taccgatcta taaccaatat tacaattcac
     3901 ttgccatact ttgcagactc tatatagagt gagagcatgc ttgccgcgtc caggctcgct
     3961 cactgcgaac tttttgcgat ctctaggact tcgcgctatc catgtatcaa ttctgccttt
     4021 cggttcagga ggcattcccc tcactattgc aaagtattct tttctagcct ggtgttctct
     4081 aaactgactc gataagaatt cctgtacaga agctgatcgg gctacaatga ttactccaga
     4141 agtgtcctta tcgagacgat gcacaatacc agcacgggta ggagaagtaa tcgatgaaga
     4201 atccttaatt tgattatctt ggcggacatt gttctccaac catagtacag catttgcaag
     4261 ggtaccatgc caattgccag ccgcaggatg agtaaccatt ccctgaggct tatctattac
     4321 atagacatct gcatcgcggt aaataatctc aagtggtatg ggttcaggct ccagttgttc
     4381 tgaaatggct tcgttccatt caagacgcaa gtggtctccg ttttttagtt tatgagagag
     4441 cttgacttct actccgttgc agaaaaggcg agcgttgcga gccttgagct gagaacgcgt
     4501 cgtgagctta attatctctg agagaaatct atcagccctt atggccacag ataaattggc
     4561 tgttccttcc caaaccggag ataccaattc tgtcttttgg tcttcttttc tattacactg
     4621 ctttcgcggg atggccatga actcgctctc catattcgat attccagaga aaaagcccac
     4681 aggccggggc cgtgggccct gccttgcttc gatctttgga ttcaagaact tctcgcatca
     4741 tgacttctgc ttcgcaaggg ttttttgcct tcttttcgag ttctagaaag gttcccacca
     4801 gagagcgaac ctgacgccat agaaaagcgt tccctgctat ctggtaaacg agcaaggctc
     4861 tttccaggaa aaacacggac tcgcttatga atcggcattt tgattcgctc ttgtcctgtg
     4921 cagaggcaaa ggtcgagaaa tcgtgctccc ccagcaccac aaaagccata gaattcaaaa
     4981 tagaaagact aggatagtga ggggaaaaat gagtgtagcg aagcacaaat ggatcaggag
     5041 ccattccaca gctcataaaa tagcgatatc gtctcaaaga agcgtcgaac ctggcatgaa
     5101 agtcaggctt tacttcttca gaagagagta cgcgaatatc tcgcggcaag agtctattca
     5161 aggccgggac gaaacgatcg gctggaatcg aagcgatatc tgtatgaaaa cctgcaactt
     5221 gtcccatagc atgaacgcca gcgtcagtcc gtgctgatcc tatgactcgc acttcgtgtt
     5281 tatgcattat caaaagcgct ttttcgagtt cctcttgaac ggatcgtccg tttttctgtt
     5341 tctgccaacc ggaaaaatcg ctgccatcgt aagcaattac gagtctgatg gttcgtaatg
     5401 atggttttga tgcgcaaaaa ggattttcct gggcgtgaat atgctctgcc atctgctagc
     5461 ttcccttttc taagattact atggcaactg cattgtctcc ttcgtgagtc atagagatat
     5521 gaatctttcc tccaccatgc cgctcgaact cgcggcgcgc gttcccttcc aggcgcatta
     5581 tgggccggcc cagatcatcg tttacaaccg cgacctcctt cagtgcgaag ttggcaaggc
     5641 cagaaccgag cgctttgcca aaggcttctt ttgcggcaaa gcgagcagcc agggaaaggg
     5701 cagcgccctt tttgcgaagg ctcgcggtct cgatctcggc tggatggaag aatcgcttta
     5761 gtatccctgg ttcattcatc catcggcgaa tgcgatcgac atggacaata tcgataccaa
     5821 ttccggaaat catggatcga ctccttgtaa tttcaatgaa aggcaggaag aactggcgcg
     5881 ccatgcaagc gaacattgag cgccagaggc tcgtatgctt cgacttcgaa gccctccgga
     5941 gcgcttacag taagcggtat agagtataga ccaggcttaa ctatgccctt gaaatcggct
     6001 tgaaggctag cattgtttgc caaagtctca tcctgtccag gatgtggtac gagggttata
     6061 tttccagatg ggagcggatc agagattata agcgatggat cgagattgtt aatgaatata
     6121 ggcacattct ttaatgtaag ccgtctttcc tgcatctgaa tcttcgcaga gaactttacc
     6181 gtgcttgtgt cgacaagcct tattaattca gaaggcttaa caagaggcac attaagagaa
     6241 aaatcgctgg tctttccgct aagctctatt atatctgtgg aaatatcctt gatactgcta
     6301 accaaagtcg ttggcccttc tatagatatc tctgatggca agatttcata ggagatcaat
     6361 acatagcctt gttccagaaa acctttaaag gtaggagtaa tagccacgcg ctttgcagcc
     6421 aggcgctcaa aactgatggg tatttctgag ggatccgctc ttaactctaa gggatcgata
     6481 ttcagagcat cccctcgcct ctcgagtttg accggaaccc tgaatacgcc ttcggagcca
     6541 taacgtgcca gatcaagact cgcgactata tccgattccc ggactctata gatttgatca
     6601 cggtcaccgc ggattatcac acggacagtc tttggatatt ccgtggcagg cacatagctg
     6661 tgcccttcag agatgttcag tggcacgatt ataacacgct gttcgagacg ggtgagattg
     6721 taaagaaaca ttataaaaat cgccataaca agagaaatga ccttagccgg ccaattctcc
     6781 agcaatttgg aaatccggga atcaaggctc attggcaacc tcgtttccct ctcttctcag
     6841 attctgctct gagaagagaa tatgaagacg ttccagaatt tcttctgagg aaagatcata
     6901 atagagcttt gagtcataag cgagagagag ggctccggtc tcttcggaga caacgagcac
     6961 aaccgcatcg gattcttcgg caatgccaag cgcagccctg tgccttgagc caaaagtctt
     7021 gcggatttct tgctgatcgg aaagaggaag aaaacaaccg gccgccgcaa gcctgccctg
     7081 tgagatgatt gcggcgccat catgcaatgg gttgtcgtgt tcgaatacgg atatgagcaa
     7141 gctggttgag attattgcgt caaggggaat acctttctca atgtaagagg ctagcccaac
     7201 tttccgggcc atgactatga taaccccgcg ccttttttcg gcaagataaa cagaagcaga
     7261 taccgcagct tcaatccagg agctgcgagc catagatttt tttccaaaaa tcgattggcg
     7321 gccaatttga atgaatatcc gcctgagctc aggctgaagg ataatggcaa gacctatcac
     7381 caatcctggc gccagtatgt tcatgatcca tgtgactgta gagagcttta ggaaaaaaga
     7441 tatgccatat atagcgagaa gaattagcaa accgcgaata agatagaccg catcggtcat
     7501 tacaagaagc tgataggtct tgtaaatcag aaaagcgaga atgcctacat cgagcacagg
     7561 ccgcacatat atagtaaaga actcgatcaa agtcggtgca ttcattgtat cacctcatga
     7621 aaggctcgaa gcgcgtccac agtctctgcc acatcgtgaa cgcgaagaat ctgagcgcca
     7681 tttagccagg cagccaatgc ggctcctaaa ctgcccgcga gcctgctagc aacatccctg
     7741 ccggtcagat ctccaatgag ctttttgcga gaaagaccaa tcaaaacagg gtaaccaagc
     7801 gaagcgatat gctcaatgcc cttcaaaagt gaaatattgt gttccttaag tttgccaaat
     7861 ccaatgcccg gatcgaggat tatcctgtct ggggatatgc cagcttcgat tgcacgatga
     7921 cttctgtctt cgaggaaaga gcgtacttct tggaggcaat ccttatagta aggatctttc
     7981 tgcatgctct gcggagttcc ttgcatatgc ataaggatga ctgctgcctt ggcctttgac
     8041 acggtctcga tcatacccgg tctttgcaat gcttcgatat cattgattat atccgctcca
     8101 gcagcaaggg ctgcttctgc aacttcgggg tggcgcgtat cgaccgagac caccgcgtcg
     8161 ctcaaggctc tgaagccatt aagcacagga agaattcttg atagctcctc ttcggaatcg
     8221 atttccgtag aacctggtct cgtagactcg gcaccaaaat ctatgattga tgcaccttgc
     8281 tttaccatgt cgagggctgt ttctattgca gctatcgaat caggtcttct gctaccctcg
     8341 aaaaaggagt cttgggtaat attgacgatg cccatgatac aggctaaatg ttcctgcgct
     8401 ctgccaagct cgagtgagcg cccgttcgga agtcgtagcc tgttcacatc atgctttagc
     8461 tgcgttctat ttgcgctaat gcgcatgctg tatctcgctg gaagtagata gtgttccaga
     8521 caagtctgtc ggaattcctg ccgctatttg ctgcttcgag atatccagga aatccaatag
     8581 ctcatgcaga gccttctgta ttccctttgc gtcgaggctt gcgcctgata gaagttcctc
     8641 tctggtcgct tgaggaggtg gaaccgaacc aaaccccagg gatgatattt tgatctttat
     8701 tcctctgcgc attagtattc ccgccacaga ctctccaaga ccgccggtca gcactccatc
     8761 ttcgattgtc aagactcctt cgtatcgaga gcagattttt acaaggtatt cttcgtcgat
     8821 tggtgatatg aatcggacat tatagacatc tgcaaaaatc ccttttattg ctagttcatc
     8881 cgatacatca gtcgcaatat atgagagcgg cccaacagcc aatataagca ctcttgcatc
     8941 ctcatgctca cgcataaaga ctcctcttcc aatctcgagc tctgctattg gaagatcagg
     9001 acaaggtgag aaagccttgg gatatcgcat catacaaggc atgtcgagag taagagccag
     9061 atctaagaaa gccaacaact cctcaccatc agcaggagca aagaacacca ggttcggcat
     9121 ggctttaaag agaacgatat cgtagattcc ctggtgggtc tcgccatctt cgccaactgc
     9181 tccagcgcga tccagagcaa acaataccgg cagcttagcc agcgcaactt cctggaacac
     9241 ttgatctact gctctctgca taaaagtgga atacacggcc accacaggcc gcaggccacc
     9301 ttgcgcaaga ccagcagcga atgccacagc atgctgttcc gcaatgccca catcgtagac
     9361 cctatgcgga tagtgctccg ctagctttga aacccccgtt ccagaagaca tagcggcggt
     9421 tatagcaacc atacgcggtt ctttctcggc acatgctatt agagcatttg caaaacagga
     9481 tgtaaaactt tctccgtggc tggtcgtccc gggccctggt atatcagggt aagctggaac
     9541 aaggccatga aaactctctg ggtcttcttc cgccttttcg agccctttcc ctttcttcgt
     9601 aacgacatga acaacgaccg gcctattcag attacggata tgttcaaaag catttatcaa
     9661 tgagacaaga ttgtgcccat cgataggtcc aacatattcg aatccatagt cgacaaagag
     9721 attctctttg aaaaatatgg cctttatggc tcgcttggtc ctgttgataa aggaaaaaat
     9781 ggctccacca atacgaggta tcttcagaac catcgtatcc acacgagaac gcaaggattg
     9841 atattgtaca gaggctgaaa gcttcgaaag atatctcgat aatgagccca cagagttgga
     9901 gatggacata cggttgtcat ttaagacaat gataagaggc agactcaatt gacccgcatt
     9961 gcttagtcct tccatcgaaa gacccgctgt aagggctccg tcgcctacaa ctgcaatcac
    10021 ccttccggca tgacccatac tcaagcgagc ctgaagaagc ccaagtgccg aagagatagc
    10081 tgtggaagaa tgcccagtgt cgaaaatgtc gtgtttgctc tcttttcgct ttgggaaccc
    10141 ggatagaccc ccatttctcc taagagttac aaatcgagcc gctcgacctg ttaaaatctt
    10201 atgagaatag cactggtgcc caacatccca gactattgca tcgtatggcg attgaaatac
    10261 tcgatgaagc gcaatagtaa gctcaacaac cccaagattt gatgcaagat gaccgccatt
    10321 gcgctgtacg gtctcaatta tgctaagcct gatttcccgt gagaggcacc gcaaatcagc
    10381 cattgatagg ccctgcaggt cggctggtcc agaaatctga ttaagcaagg acatttcgaa
    10441 acagtctatc acctatttcg gaagatttcc agcgggattt taagcgatct ttgctggaaa
    10501 ttgtcatgcg cctattgagc agtcttcgca ttgagcagcc ttcgcataag gaaatcgtca
    10561 aaaccttcta agtctgcttg tgtccacaag cgcaccattg agactattga accttacatc
    10621 tcccttgaca atcacggcta tataatttga aaatggcatc atggagattg aactcagaat
    10681 actgcatcta tgtcgaaata tcgactatgt ccatatcgat gagcctctga ttatcgcggc
    10741 aaaggctcta tcggatattc tctgttgtca gtctgaactg ctgctggaat ttgaagaaga
    10801 aggactttcc acgatgactg aagacggacc aaaggtcttt gacacgctcg agccatcagc
    10861 agtatatcgt ttggcagaaa gtaaatatat agaacaaacg tctctaaata atttagatct
    10921 cggaaggact tcgccgttct ctcttggcat aggagagtac atattttgtc agcaggatat
    10981 ggattgcaca gaaggggaat ggcgcctaaa gtcgctcctt gaagaggcta tacgcgaagt
    11041 ctggtggcaa agggtcgaat gtgaaggctc atggtttttg cgccttgttc ctgaggatga
    11101 ccgcgtagcg gttcaggctc ttagaagaat gaaagcaaaa taagcctaag tctgttcaag
    11161 cacggcatag atacgccgca ctcccgcact cgaagactgt tctttaatga tcttgatttt
    11221 tcctatctca ccggtatgcg aaacatgagg acctccgcat acttctttcg aaaaatcgcc
    11281 catggtgtaa acctttacaa taggctcata tttctcgcca aaaagcgcaa tagccccgac
    11341 agactttgct tcctctggag gcatcatctc cattgtcaca ggaaggtctc tggcaatttg
    11401 ttcatttaca attcgttcta cagcagcgat ttgctctggt gtcataggcg caggatggga
    11461 gaaatcgaat cgtaagcgtt cggcggtgat gttcgaaccc ttctgagcaa catggtttcc
    11521 gagtaccaga cgcaatgctt tatgcaaaag atgggttgcg ctgtggtaac gcgttacaat
    11581 ttccgaattg tctgccaatc ctcccttgaa aatctgttcg cttcccaccc ttgaaagctc
    11641 ttggtgcttc ttaaaggctt cctcaaaacc cagcctatct accctaagtt catgttctgc
    11701 tgctagctct tcggtaagtt cgatgggaaa accgtaggta tcatataact tgaaggcaag
    11761 cctgcccggc atgattctat ctggattctt taggagattt ggcaggattt tctcgaattc
    11821 atgctctcct ttttgcaagg tctctaagaa tttcttttct tctcgctcga gctcttcgta
    11881 aattcggctg gcattctcaa gcaattcagg atatgggccc ttgtagatgt cgatgacagc
    11941 ctgagcaggc ttggaaagga agagtccatc tatgccgagc tttcgtccat gacgaacggc
    12001 acgacggatc aaacgccgca acacgtatcc agccccaata ttggatggca aaacagcttt
    12061 agggtctcca agaataaatg tcgctgcacg aatatgatct gcaattatcc gaaaacctcg
    12121 atcgatttcg ggatcgccga ctgaggctcc ataagtcttg ccggtaatag attcaatgac
    12181 cttcaagatt ggggtaaagg cctcgatttc atagaccgac tttttcccct gcagcatggc
    12241 aacagtacgc tcaattccca tgcccgtatc gacacaaggg tgggcaagaa gctcatagct
    12301 tccatctgcc ttcttattat attgcataaa aacattgttc cagatttcaa agtacttgcc
    12361 acaatgacag cctggtctgc aatccggacc acagacaggt gcgtctgtgt cgataaatat
    12421 ctctgtgtcc gggccgcatg gacctatctc tcctgccggg ccccaccaat tatcctcgcg
    12481 tggaagaaaa aagattctct cttcagggat gccgacactt ttccagattt cagccgaccg
    12541 atcgtctctc ggcaccgatt cgtcgccggc aaatacggta accgagatct tagcaggatc
    12601 tatgcccaga tactcagggc ttgtcagaaa ttcataggac caatgtatgg cctcattctt
    12661 gaaataatcg cccaaagacc agttgccaag catctcgaaa aaagtaagat gactggagtc
    12721 tccgaccata tctatgtctc cggtgcgaat gcacttctga tagtcggtca gtctcttgcc
    12781 agcaggatgt ggttcgccaa gcagatatgg cacgagagga tgcatgcctg cggttgtaaa
    12841 aagtaccgtt ggatcatttt cgggaatgag cgacttcccg ctaatctcag catggcctct
    12901 agccttgaag aattcaatgt attttctgcg cagttcatcg attgtaatca tagcgtggca
    12961 agtgtaacga aaggcctatg agcaggtcaa tgagcaagaa tctcaatttt tgaaaactac
    13021 tatgttgctg aatgaatgtt cattcattat aataatgagc gatagtatca aatgtgagga
    13081 gaactatgcg aatcttagtg actggtggat ttgggaatgt tggaacaagt gcagtcggtg
    13141 ccctgttggc caaaggccat gaggtgctta tttttgagca accttctgct cgttcgaaaa
    13201 agcaaaaaag aacacgagaa ctgttgaaaa atgcgcatca taggctgacg atcttctatg
    13261 gagatattac tgacaaaaaa agcctacagg atgctattta ttctgttgga gtcggaggaa
    13321 tagatgcgat aatccatctt gcagccataa tacctccctt atccaatcaa aaaccattac
    13381 tcgcagaaga aatcaatgtt gggggaacga gaaatatcat agaaatatgc aagagtcttg
    13441 cggtgcctcc acgaattgtc tttgcctcca gtgtctcaat atacggagat aggcttgcca
    13501 atccatggat ctcggcgacc gatcctcttg atcccaacga caactatagc atcacaaaga
    13561 acaagagcga gaacctgctt cgagaaagtg gccttccctg ggtaattttg aggttatcgt
    13621 atgtagtatc cagcgattgg cttccctttg atcctatgct cttcgacgtg cctccctcaa
    13681 cgcacttcga ggttgtgcat accgaggatg ctggccgcgc ttttgccatg gcttgcagcc
    13741 tttccaatat tgaaggtcga acattcaata taggcggagg accagcctgc cgaacaactt
    13801 accgagccta tctcgatagg cttatgaggg cgtttggcct tggaaattct tcatttctgt
    13861 gcgatgagat atttgccaga ggagactttc attgtggttg gtttacagat tcggaagagg
    13921 ctgaaatgct actgcatttc agaagtaaat cgctcgaaga ctattattca gaggttgaat
    13981 ggcgcacaag gtttattcgc cctttgggca gcatagccgc aatggccgta aagccctgga
    14041 tccgctcact cagtccctat cgggcaaagg ctgaccgagc gcattctcca ttaattgtct
    14101 caggaaagcc cgaatatcgg cagcattgat atcgatatct gtctttgaag taatcctgat
    14161 cgtctcgagc gctcccaaaa gaaataccat tatgccaatc cgatagaatt tggcgtccag
    14221 aggaacctcg agggaaacag aagagtcttc gaccaacttg gagatcatgg aacttaggta
    14281 ctgtagcttc ttttcaagcc tggcattgga gcttgcccca gctagcaaaa tcataatgag
    14341 ttcaacatcg ctgaagagct ttggaagcgc ttcatccacc aagaaggaca atgcgcaaag
    14401 cgattgtcct ataccatcgt cctgctttcc agactccatg attgagacca tcctgtcttc
    14461 tacccaaacc cgatactcgt cccagccttc ttctataatc gtctctataa gcgattgttt
    14521 cgaagggaaa tatgtataaa tactaccaac ggaaatgcca atctctttgg ctattccatt
    14581 catcgaaaca ctctcccctt tagtagcaaa aagcctcttt gcagtctcaa gaataagcgt
    14641 tcgcttctct tttgttttga cgcgagccat tatctataat ctcctttatt tgcacaatca
    14701 aaacacaata ctatcggaaa atcaacacat tgcaatatat attcatagga ggttatcgta
    14761 atttagctaa atattaattc tattatgaga taaatatttt cgcgctttgg agcattgaca
    14821 ttttttataa atcgacatta ttatcttgac aactttttat ttatgggcgc taattatatt
    14881 gaggttcggg aggatattcc gaatggagtc tgatagtaat tcaaatagta ttaaaagaaa
    14941 aagagtgata aagcttgtat ttattgcttc tttctgtatt gcattattat ttgttatttc
    15001 tagagccgca aggttatgcg aaaccatttc aatttataaa tcaaataata aatcatcaag
    15061 tattatgttc tcaataaata tcggtaatat caataattat gatgatccaa ttctgaagct
    15121 ttatactaat cctaagacaa aaaatgaagt ccttgatttc ttttctgcta taactaattc
    15181 ggaacgtgta gctagagcga ttttggataa tgccgtggct cataatatgc gacctagtct
    15241 tgcttttgcg gttgcctatg tcgagagtcg atacaatcca agagctcgtt ctaccaatcg
    15301 aacgggtaca ctcaatagtg gtcttttcca gctcaattca tcggtatatg gcaagataag
    15361 cgaaaaatcg atctacgatc catatcaaaa tgcaagactc gggataagcc atcttcaaga
    15421 atatatcaat ctaaatggcg atgaattctc tgctcttgca gcatataatg cgggaataac
    15481 cagaatctca gaaaaaggca ttcctggtgt gacctttaga tatatttccg aagtatctat
    15541 tatggaacac aaaattatga acttttttat cgctagcatt gctcttagcg aatcaatgaa
    15601 gctttagtcc tctttgttct caatattccg ttcaaaattt ggattcagca tattatcgac
    15661 aaaaggagta agcgcttcct gtaatcctgc cgcttccttt cgcccaagta ccacgagacg
    15721 aaatacagca gcctctacaa gtgcaaagaa catctccact gttcctttta cggaagaaga
    15781 ttccttgaat tcaccacact ttatgccttc gataacgata tctgcaagaa tatgacgcat
    15841 gcggacggtt cgacgcctta ctttttcgtc tggatttcct ccagaagctt ttagtctcag
    15901 cagataatcc atgactaccg agagcatttt cgattctctc tcgcaggctt caaccaaaat
    15961 ttcgctgatc ttggtaattt tctcaccatt tgttaggtac tgatcccggg caataagcgc
    16021 tatctccgct tcgagagagg agagaaaacg cttaatactc tcgttgaaga tctgtttttt
    16081 gttgtcgaaa tagagataga gtatcgttcg agtgatcccg catctctcgg cgatcttctg
    16141 gaaagtagta tctgcataac cttcttctac aaacacatcg agagcttttt ccagaatttc
    16201 ctgctttctc ttttcgtggg cgacctggct agacaaatcg acctcctttg tcaaattgtt
    16261 atcataacag aagatctgat cttagtatat cttccgatat gaattcctgt tccttgcgtt
    16321 gcttgcggca taaattacta gtatagttga taaaggagct tatcgatggc tttacagatc
    16381 ctcagagaat tccgctctca aaatattcct cccaagcacc tttggatgcc ttcgggagct
    16441 atctctctgc ccgatgccgt atccgcacgc tttgtggctc agaaatataa gctgtctgca
    16501 ggagagctga gagctctttc tttgatcggt gaagccttca ggattatcat cgacctatat
    16561 cgaaagcaat attcgaagtt gctagaagaa attgcgctta aagcgttcgc ctccacagaa
    16621 gacaacaagg cgctttggga ggtgctccag gaattgatta cagaatttcc ccccgctccc
    16681 atctacgatg gtcttgccga acccaaggat tggctaaaat ccctgagtcc tgttggcgac
    16741 gactccagta agcctaatct agaacttgcg atagagcagc taattttggt tcgtcttttt
    16801 aacgagaatc cggcattctg gccatatcga tcactcttcg atgacggggt ctctccggca
    16861 ggggccacat tgccagattc tatctctgca aagactcctt acctacaggt ttttgctcgt
    16921 ctcgaagacg ctctcaaaac cttgccaggc ttatcttatg gtggcgggaa aactctcgac
    16981 ctcataaatt ttctgcgaga accatccagg cacgcacctg cttcgctaaa ggaccagctc
    17041 gaatggataa tcaagaattg gggaactctt ttgggcgatt ttaagctcag cctccttgct
    17101 ggaatagaca tgatcaacga agagaccaga ccgcattttc ctccagggcc ggggcttgct
    17161 gttccctatc agtatagaag cagcttccat gaatatgaaa aattcagccc cgacaagaac
    17221 tggatgcctt ctcttgtgct tattgcaaaa aatgcccttg tctggttaca ccagctttca
    17281 agaacttact caagagagat atcccgcctg gatcagattc ccgaggaaga gctcataatc
    17341 atggccgagc gagggatcaa tggattatgg cttatcggaa tctggcagag aagtccagca
    17401 agcgaaaaaa taaaaaaact ctgtggaaac tcagaagcag ccgcttcggc ttactcccta
    17461 ttcgactatg aaatcagccc tgagctcgga ggctgggagg cactggacag gttgcgagag
    17521 caatgtggac aatatggcat aaggctggcg gccgatatgg tccctaatca tacggggatc
    17581 gactcgctct ggatacgtac aaagcctgag ctattcatga gtctgccata ctgtccattc
    17641 cctagctata gcttcaatgg tcccgatctt tccggagatc catctattgg gatttggctc
    17701 gaggatcatt attacaatcg cggcgatgct gcggtcgtct tcaaaaggct cgatcgtcat
    17761 actggagaag tccggtacat ttaccatggc aacgatggaa caggcatgcc ctggaatgat
    17821 accgcgcaga tagactttct aaacccggct tcgcgcgagg cggtaaaaga gcgaatcctg
    17881 tctgttgcgg ctcatttcaa cataatccga ttcgatgccg cgatggtgct cgcaaaacaa
    17941 cacataaggc gtttatggta cccggcgccc gggtctggag gcgcaattcc ctcgcgctca
    18001 gatcatgcta tgagcgaaga ggcctttgac aaggcaatgc caaatgagtt ctggcgagag
    18061 gtagtagacc tgtgcgcgga aaaggcttcg gatacgcttc tgcttgccga ggctttctgg
    18121 cttatggaag gttattttgt acgaaccctg ggtatgcacc gcgtatataa ctcagccttt
    18181 atgaacatgc tcaaagacga aaaaaactca ttgtatcgcc tgaccatcaa gaatacacag
    18241 gaattcgatc gtgatattct aaaacgtttt gtcaatttta tgagcaaccc agacgaagag
    18301 acggcggttg ctcaattcgg caaaggagat aaatacttcg gtgttgccac tatgctagct
    18361 actatgccag gattaccgat gatcgctcat ggacaaatcg aaggctttac agaaaagtat
    18421 ggcatggaat ataagcgctc ttattgggat gaaacgccgg atagagatct catcgcacgc
    18481 catgagcgcg aaattttccc gctgcttcga atgcgtcggc ttttctcaga agtcgaaaac
    18541 ttttatttat ttgattatat gcaagatgat ggaacaatcg acgaaaatgt cttcgcctac
    18601 tgcaatggcc agggagagcg gcgagttcta gtattctata acaaccactg ggagcgtact
    18661 ttaggccgaa ttcatacctc ttgcgctttt gccaggaaga ccgctgatgg caaaaaacaa
    18721 ctaaaaacaa cgagtctagc caatgcactc aaaatcgatt cctctccaaa aaattatgtc
    18781 atcatgcatg agattcgttc cggactatgg tatatattcc ggagcgaaga tattgcttcc
    18841 cgcggtttca aattagctct ggaaggctac cagaacaagg ttttcatcga catcatgaat
    18901 gtgcacgata ccgagggcag atacactaag ctctttgaaa tagttgattc gagaggaata
    18961 gcggatctgg atgatgcctt gcttgaggcc gaccagcccg agctctatcg atcgctccat
    19021 aatgcgataa acagcctcag cagcatagag acgattcaaa atctctcgca ggaagaagct
    19081 attcaaagag cgactatttt ttcggagatt ttcttttcga ggctttgtga gattgccggg
    19141 agtgatgata ctcttgccgc atcccgcagc gactcagtcc gatctgcaag ctcatggctt
    19201 aagacagtgc tcaagctgct atatgggaca acagagtccg atgcaggcat tgctgcagca
    19261 actctttgca ataacagcag tgcttccaat tcggaatatc agcaatatag gctgattctc
    19321 ttaatctatt catttatgag gtctttggta aaagctttcg ccgcagatga actcaatgag
    19381 gaagtttctc gcgttgtaaa agaatatcga attgcaaaaa aactcactga atctgcagtc
    19441 ggactgagtc gcagaaacga tagtcacgac actaaatacc atgtctcaat atgcgcagag
    19501 atagcaatag cctgggcgct gagacaagac aaacttttct ttgacacaag gagaacaatc
    19561 gattcgactc tgactccgaa caagcgtgca caagaaattt gtgcctgggc tttttccgat
    19621 cctttaatgc gcgaagctct caatataaat cagtatcgtg gaacagaata tttcaataaa
    19681 gagcgttttg aggcttttgc aagtctcttg cctgcttttg catggatcga ttcgattcaa
    19741 gaagaaacca aggaaagaga atggaaagaa gatccatcat ggaaggaagt tgctgaaata
    19801 ctgaaagaga atgcgattgt cgcaggttat aggacaggta taatgctcga actcatggca
    19861 tctgtagcgc aaacatagag atgcgcttaa gacaaagacc tttttaggaa agatcattta
    19921 agttatgcca aaatctgtag attcgaccaa aatagcagag ggatataaat cacttttagg
    19981 aactccgctt ggtcgcgata tctcacaaca cctgcaagat gagaatattg tctttgtttt
    20041 tccttctcaa gataccgccg attcctgggc acgcgccgtt gtatattccg aaatatccag
    20101 agcggtggct ctaaatcgtt ttttgggttt tgataatttt ctgttatatg tggcaaaggc
    20161 taatgagcaa gatgatctat cggtgctaaa gcctatccac cgctggatct gggctatgca
    20221 aatcctttct aaagaacaga aaggagaaag aaagcccggt aaaagactat ctactttgct
    20281 tccttctatg gaaatctcga ctaagaatct tacaaggctc gtacgcctcg cgccctatct
    20341 attcgaaata gaaatgctaa ccaaagacag cgctgtgcta gatgcgtctc catgtcgctt
    20401 cataaaagaa gaatttgaag aaatgagatt actggctgaa gactacatcg attttcttga
    20461 tcgcgctcga cttagggatt cgcataggcg ccctttggtc ttgccaccgt ttatgaaagt
    20521 ccgagcttat ggactcagag atgattttat ccgatttgga ctgatagatt cagaagagtt
    20581 gggcgatgga aatatcaatt tcagggggat tttaggcgag gagactggcc acgagaaatc
    20641 cttaagtgcc aaagaaagga ttgctttccc ggatatcttc acacaggaaa gaggagaaaa
    20701 ggctccgatg ctctaccatc aattcaactc ctcgcttgtg gaaatcgaat ggatactgga
    20761 agaaatatcg aacgaaatcg aacaaggcat cgagcctgaa gatatcgcta tatctgtatg
    20821 tgggctaact gcaaaaaagg aagcgtggat tcgtcaaatt gccgcggaat atggtgttcc
    20881 agtctctatt cgccggggta agccactatc tatgtcgcca tttggcaggg tattgatggc
    20941 aattcagaat gcggctcgtg aaggcttgac tctggaaagc ctcgatgcat ttgccgatct
    21001 tgccaatatt acaggacggg acccggaagc ctggaaagct ctgcgagata cggccttatc
    21061 attgcgcctt ccttcgcctt ctccgagtgc cgcatacata cacagtctat ggaaaggcgc
    21121 ttttgggaca gaccaatgcc ccgaatcgac ggctcagatt tatcaaaggc tctgggaaag
    21181 catcttgtcc atagccaggg cagactcttt ctccaggctc tatgatcaaa taatcggttt
    21241 tctggagcat tggatcgata caagtggatt cggagccaat atacagaccg atcgctcgat
    21301 gcgtcttact ctagatgaac tacagagttg gatagaccat gaaaacattt tcacagacct
    21361 ctctattccg ccatttgagc tctttttgac tgtgctcgag tcaaaaagct atattccttt
    21421 ggtggaagag aatgcagtgt atatatacga ctttgatacc acagcgggaa ttgcagtgct
    21481 ttcccattat gtcgcgggtg cctcgcagga gggtttggct tcttcgctca aaatcaagag
    21541 cgcccttcca ccggaacttg cgggcattct ccagcaagaa aagaccgata aggctcacga
    21601 agtcataaat atgcaccggg tttctccaac ccagttctct tttgcagcca agggatttga
    21661 agattatgag acggcctatc cgctattccc tcttccaata atgattgacc ccaatgcaga
    21721 gcgtggactt cgagctatct taatttctaa agaacaaaaa agcgctacag agagctattc
    21781 tcatccagaa cctatagagc caacagtagc acgcagtttt ttgaacgcaa ctaagagctt
    21841 cgattcggaa aagaagtatg cgatttttag cccttcttcg ctcaaagagc tatcgcagtg
    21901 tggctttcgc tggtttgcaa gcaagatcga cctggtagat gtctattcaa gggacgatag
    21961 cgccatagcc atcggcaact tatatcatag cgcctacaga cgcgccatcc aagcacttcc
    22021 gcaaaatttt aagaatgccg agacggatga ggaatttcgg aagactataa actcagccct
    22081 aagtaaagaa gccaaaagaa ttctcctcga gtatggcaat ggacttcagc ctattttgtc
    22141 cgcgcttttc ccgcgcgcaa ttcacagact aaatgagctt tggaatttcg aacgaacgat
    22201 gttttcggcc tatgagcgcg aagggttcga aatgaggctt cagcatgaat tttcggaaga
    22261 agaagcaatc cttgagggca ggatcgactg ccttttcagc aggcaagatg agagcttagg
    22321 aaatataaaa tgttatgtca tagtggatta taaaaagaat cgtataccat ctatatctaa
    22381 aatgagagtc ggtgtaatgc cttctgacta tgaagagttt gcatcctatg acgaaaacca
    22441 agagaaagaa gacggatatg acagcagtgc attttttctc gaggaaatcc aggtaccttc
    22501 ttatactctc ctggtggaaa cctcttgggg tcgcgtggaa ggagctttct attggtccat
    22561 cgaaaaagcg gaagctgtcg gatatatcag gcctcctaca gtgcctaaaa tgagatcggc
    22621 gtatgcttac agcaaggaaa cggatttcca gcgttccgcg ctccgcgata tgctgcgagt
    22681 ggcttcttca aaggtgaaag ctgggaattt tcttgatctt gcgctggatc gcgcggtctg
    22741 tgaggactgt gagttcaaac cgctttgtag attctggtat tttctggagc tataatgata
    22801 tcgcaccatg attccttcga tcttgaagcg ctcaagctcg acgattttca aaaaaaggcc
    22861 gcttataccg aggatacagc catcgttacg gctggagcag ggacaggcaa gacaagagct
    22921 ctggtaggcc gttttctata tcttgtgctc cagaaaaatg tggatccaga gcgtatattg
    22981 gcactcacct ttactcgtaa ggcagctgcc gaaatgttcc agagagtcca acaggcatta
    23041 agcccatttg ctaatgaccg tccgaatctc gccagaagtc ttatgcaaac gcatattcag
    23101 acactagact ctttctgcca tgaaatcgtg gctcaagctg ctgttctata tggctatact
    23161 ccagattttc agatcgacga gagtaagtgc gcacgcattg cagaaaagac cgcataccga
    23221 tttgtcctcg acaatcagaa aaaagatggg atcgaaagcc tagtttttgc ctttggcttt
    23281 caagccgtgg ttcaagacct ttttgcatct tttggaaggg caaatgtcga accgcattgg
    23341 caaggcaagc aaatctgtca cgcttcggct cgaatcggag aacaggaatt caagaaagcc
    23401 gctgaagaaa ttgcagagaa aatacacgat attgcactca atattctgga aatcgctaac
    23461 caaaaaccag gcaattacgc aatacatggc ttcagagaag gtacgaatca agctattcgc
    23521 gcagctagaa cagtctcatg cctttcttat gatcttaaag gtttgaaaga cttttcggat
    23581 tatatggaga agccggaaaa tgccataaat cttcaaagcg tgggccgaaa tgaaacagaa
    23641 tccgcaatca aggagcttgc caaagagctc aaaaacaagg cgcttcgaga cagaatctct
    23701 cagctcgtgg attttgagaa gttcttgccg agttattacg ccatcatgga tcgcctcgat
    23761 gaatatgcag acatgctatg tgaagaaaaa tgcatagcca atataatgaa ttacaaggat
    23821 ctaggcgccc tggcagtaga cattctgtcc aatcatgaag acaggcgcat cttctgggct
    23881 tctcgttttc agtatatcct catcgatgag ttccaggata acaacgagct tcagaaacaa
    23941 ctcctttttc tcttgagcaa agagggcaaa gagatccgaa gaggaaaact gttttttgtc
    24001 ggagatgaga aacagtctat ctacctcttc cgtggcgcag atgtaagcgt attcaagggg
    24061 cttgccaatg agctaatggc ggctccactt tcgctcaacc taaactatcg aagcacaaac
    24121 caactgatcg aattcttcaa cagcgtcttt cgcattgtta tgctaccagc caatccggag
    24181 aatccgcagc ctttcgaggc tctgtatcag gatatggaaa ctataagcac agagaatgct
    24241 aatatcgaag agtccaaagg cttttcctcg cggatcgagt atcattgcat aacgaacaaa
    24301 ataccggccg aagaggaagc ctgcggcatc cgttcggcaa aggagtcgat ggcttttcgt
    24361 ctggctctgt ggatacgaga cgccgttgaa tcctcgaacc ccctactcat cagaaaggaa
    24421 ggggaaacac aggatctggc aataacagaa aaaaaacaac ataaattatc gcttagaaag
    24481 gccgaatata gcgatatcgc agttctcatg cgcacaacaa gacgccaata cgagctcgaa
    24541 aaatacttgc gcatgctggc tattcccttt gtcagcgata ctgctgctag tctcttctct
    24601 gaagagccga tcaatgatct ttactacatc ctgcgccttc tcatagatgc caaggatctt
    24661 tttgcaacag ccgcggtact ccgcagtcca ttgtgtcgca tttcgaacga tggatttgtg
    24721 agcatcctca ccgagaaaaa gagtctggct gatctagagg cagacatgcc aataaaactg
    24781 aaccccaatg accgagattc tatagaacga atggtcttat tttatcagga gttgcgccaa
    24841 aaatcggatc acatgcctct catggagctt gtggcctata tatggaagga gggacggcta
    24901 gaggcttcga ttctctgcga tgaacaacgc ttgccatacc tggatcattg gaatgctata
    24961 cgaaccctgg cagcaaacac agaggcagag ggcgggcaca tatccgcgtt cctaatatca
    25021 attcgagatt atatgcagaa catgcatttg ttcgacacca attccgtgcc aaaagccgaa
    25081 gcgcacggtg tccacctgct cactatccat aaatctaaag ggctcgagtt ccccatagtg
    25141 ataatcccat ggaccgaagc aagcaccaac aaaaatggcg gaaaagaact ctggggaatt
    25201 cttgaatcgg ctcaggagaa aggaagaaca cgattctata cagtggacat aggatttcac
    25261 gatcgaattc agagcggctc aaatattctt cagactcagg caaaagccca gcgcacagag
    25321 aaggagcaag ctgaaaccaa gcgcctattg tatgttgcat gcacgcgagc aatagaccac
    25381 cttatattct tctctgccgc tcccgaaaat aaaccctatg atagggatag tttccatgca
    25441 cttctctttc agagtaccgc tcatctcatc ccacgagact atagcgaaaa aactcaactt
    25501 cctgctcatc tgtcgggttt gcagttattt ttcgagccgc tggtctctga caaagagata
    25561 caaggagcca gatcaaacgt gttgcgtgcc agaaaattgt ctcaaaaaaa actcctatct
    25621 gggaaagaaa aacaagaatc agaacagaaa tccaaagaag cttcctgcaa gacggcccca
    25681 aaaacagaaa aatcgttcat cgaaccggct aagatgacaa atctgccttc tgactccgtt
    25741 gccgggagcc attctcggcg tatcacggcc agcagcataa atgccataat tgcgcagcag
    25801 tctcttgccg cggactatgc gcgcgaacta tccgggcatt ttgtaagcaa acgtatattc
    25861 agcccagcag agtctcgagc tcaatctgaa gcccaagccc ttgatgaaac ttcactcgaa
    25921 gcccaaaata cccaagaatc tctcgacttc agtgcctatg ggtctttagt acatgaactc
    25981 tttgttcata ttttggctga aaagacaatg gaaaacttta ggccttctgc gtccttatct
    26041 cgtgagctgc ttggaagcac atcaccagag ttcggcgata catctatccc caagaagacc
    26101 gatcctcgct atctaagctc tataatagaa aacgcagcag ccgagcttgc cccgctcttt
    26161 gaagccccta cattctcaac tcttgtccta gaaaagaaat atagttgcga atttcccttt
    26221 ttgcttggac tggggagatg gattatcgaa ggaagaatgg acttcgtagc ccaaagtgat
    26281 gaagaagtca taatactcga tctgaaaacc gaccgaagat ttctgcctca taagtatgct
    26341 cttcaacttg ggctttattg catagctgcc caacaactct ttcaaaagaa aaaagttcac
    26401 gcagggctca tatatttgca ttttggacag attgcctggc tgaatgctaa tcctgatata
    26461 gagatcctat tgaaaatttg cgattcgata tgttttgata cacatgtcaa ttcataattc
    26521 aggaggatcg atgaaaatcc cctctaggaa aaatggtgct aagaaaacca caaaaaagac
    26581 ggagccaacc cctttgcgaa tttttcttac attggcacag atcaatacac ttacgcttgg
    26641 aggcgggtat gtgatcgttc ctgtcatagg caattccttc aagaagcagg gttggataga
    26701 cgaagaagaa ttttatagaa ttttctcgcg agcccaggtg tttccaggcc cgatcgctct
    26761 gagtacttca tttctctgtt cttataagat agcgggcgtc aagggagcca ttgccgcggt
    26821 gcttggggtt gtactgccgc cttttattgc gcttatcctc gttggaggct ttatcagcct
    26881 ctatggagat accgagctat tcaagcgctt tttgaaagga gctggtgctg tcgttccagg
    26941 ccttgtaggt tcgatgctct ggaagactgc aaagaatcgg aaatggacga ccagagcctt
    27001 aatcgaagtg gggttactca ctatattact cgttcttttc ccatcggctg ccttttttat
    27061 tttgatagga ggaattgtgg tcatatacct aggaaggctt atatggaaat cctaaaaata
    27121 tctttagcat tttttaagat aggtatagtt gcttttgggg gaggctggtc ggtagtgggc
    27181 ctcatcaagc aagaaatagt tcccagctgg atgagtgtgg atgcctttag ctcgctcatt
    27241 gcaatagctc agtccacgcc aggacctatt gcactcaatg cagccacatt ggtaggttgg
    27301 caaaaggctg gttttttcgg tgcactggct gcaaccttct ccgttatagc ttttccactt
    27361 ctggcgatgt cccttacgct ggccttttcc agatatatcc ctataaagaa agacctagcc
    27421 gatgagagct tgcgatctgg cagcatggcg atgatcatta tgacgctctg gaccctattg
    27481 ccccagaata ccatacctat gccatggata tttgctctgg cgtcctttgg cattactgca
    27541 ttcactcaaa tcaatggtgt ctgggtcatt cttggatcag gagcgatcaa tatgctcttg
    27601 ggtttgcttc catgaatctt agctccatca atcttcatcg cacgcatgga cagtaccagg
    27661 gccgcactcc gcagggggtt cgatcggtgc gggaacaata ttagctagcc cagaacgctc
    27721 gacagtgtat ttttttacaa aacctacagg cctataggtc atttttgcct gcctaagacc
    27781 cagatctcct aggtcctgct cccgattgat cagcagataa tgcttaggca gagactgggc
    27841 aaaggcttga tttataaact gatagatgcc tttatagctg tcgatcgcct tttcgaaatg
    27901 tatggcaaac atcttcccct ttgcaagagg ttctccaagg caccagccta cgggattacg
    27961 gtcgatatag taaaccgcgc cccgcaagcc caggatatta aagcactcaa tggcctcacg
    28021 agcagcggca tagtctccat ccgccccttt tgccgctctc cactcctcaa ggacttgcaa
    28081 tgcatctgcg gcacggtcct tagtcaaagg gcgctgttcg caggcatagt tgcttacaaa
    28141 gccattcact agattgcgct ttttgtgata tacgcggcca ttgagagtag ctaagtcttc
    28201 ccgattatat agataatcga aattatctct gtcctctcgc accaaatatc cacgtctttc
    28261 tagctcgatc ctctgtttag cagcctggca ttccgataga tggcgcatgt aatcgaaatt
    28321 caacataagc tcatcgaatt gttccatcga tggaaagcag cagggaatat agaaaaaagt
    28381 tctacccttg tagcgaccgg agatgataaa tttttcgtct gatatctgtg aaatctcata
    28441 gccataggta tttcggaaaa gatataaccc cgaaaaggtg aattccgata taccatctgg
    28501 cagcaaattg agagctggat agagaatatc tcttgcttct agcgtaagag gtgcaaattc
    28561 tggatacttt ggaattgtca taaggtatta taatatactc cgccacaaga accgagacaa
    28621 gaatcccata gaactatcca aatcaaaata tgcactgcgt caaagaacat ggtgtttcat
    28681 cttgatttgt aaagactccc ttgcgcaaac cataatacag atgtcatatt aaaactatta
    28741 tgatagcaat actcgattac ctaaaccgtt cctttacaga agaaatgctc cgccgttttc
    28801 tcagctatgt gaggatcgat actcagagca atcgccacgc tgaaaaaaca ccgacaacag
    28861 aaggccaatg ggatcttgcg cgccaactcg aacaagaatt gagagaactt ggcatttcgg
    28921 aactggagct cgataggcat tgttacctga tcgctcatct gcctgcaagc ccgggcaagg
    28981 aacacgtgcc atgtattggc cttatggctc acatggacac tgcaagcgat gtttcgggaa
    29041 gtggtgtaaa gcctcgtgtt ataaagaatt acgatggtaa gggaatatgg ctttccgagc
    29101 agtatctgct tgatcctggc gaattccccg atctatatga gcatgtcggg gataccattg
    29161 tggtgaccga tggatctact cttcttggag cggatgataa agccggcatt gcggaaatca
    29221 tgacggccgt cgcttatctt cttcagcatc ctgaacttaa gcatgggcct atcgatatat
    29281 actttactcc tgacgaagag acaggcaagg ggatggacat ctttcctttg gggaaagcgc
    29341 gagccaaggc ttgttatacc ctcgatggtg gtgccgcacc cgaaatagag gccgagtgtt
    29401 ttaacgcata tgccgtaaaa gcagatttct ttggcaaggt gattcatatt ggcgccgctc
    29461 gcggaagact cgctaatgca gttgccatgg cggcaagctt tatagacatg ttgccgcgtt
    29521 cagagagccc cgaagctacc gatggatggt ttggctacta ctgccccatc gaagtgtctg
    29581 gagggctaga gcatgcatgg actgaagtat atcttcgaga tttttcggct tctcgcatgc
    29641 aggagagaat agccgcactg aaagcctttg ctgcggcagt cgaagcccag tttcctcaag
    29701 gccacataga actcaatgtc actcaacagt atctcaacat gaaagaaaaa ctcgataagc
    29761 accctgaagt gctcgaaaga ctcaagaatg ccataaaggt tctaaatatc gaacccataa
    29821 tgaagccaat ccgaggaggc acagatggct ctcgcttgac cgaaatgggg atcccaaccc
    29881 caaacctctt tacaggtggc tacaattatc atagtcgctt tgaatgggct tctctctcag
    29941 aaatggcact ctcggtggat accatgctca accttgtgca aggctgggct gattagcttc
    30001 tagaggatga agacccagga atagaaagag ccagcactat tgtttcgata ttatatataa
    30061 atattcccaa taatcgtaac tttcggcgct ttaagctcta ttgaatataa tgcgcctttt
    30121 tatgaaaaac cgtattttgt ggacagaaat gaggctcaac tgatcatata ctgctgtata
    30181 aatatgggtt tacacaattg cgctcatatc ttatagttat tcaccaacat ttggaaaaaa
    30241 tgcaacagaa aaaaggcaga tgaatgtttt cttcattgga ttttaaagaa tatgagcagg
    30301 ttgcaggcaa ctcaggagca tttaagggcg ttgaaataga cctcatgctg gaaatcctca
    30361 aggattggca acaagcgcca ggcaagaatt atacacttct ggagctgcgc gatggaaaga
    30421 ctcttgtcgc ttttgcaatc atccataagg cagcagggcg taacttcaca ttcgatatcc
    30481 gttttgttgt ccttgatagc gattatcgat ccacaaacgc tatccagcat ctttttgaga
    30541 tgatcgatca agaactcctg aagaaaatgg cctttgcggt gatccgattt gaaacatcaa
    30601 ccagaaaaag agatagcctg ggagaaaaca ctctggaaaa ctcgggatat agccagatag
    30661 gacacatagt tgacttttat ggtgctaatg aggatttctt cttttatata aaggctattt
    30721 ttcgaaatcc tccaaatttt ataaagatat cgaagccttt cgaagaagat ttatgctcag
    30781 agtgcttgcc tgaagaaatc gataatcaga aataggatca aaaatatgat tgtgggagca
    30841 attgaaggcg gtggaacaaa attcgtctgt gctgttctgc aattcgatga tccaaatata
    30901 ccagaaaaga ctcctcgaat tctaagaata gagcgtatcc cgactacaga tccagagcca
    30961 accctggatg cggttgtccg attcttcaag gactcagaaa ttgaatgcgt tggccttgga
    31021 atgttcggtc caatagaatg cagaatcgaa agtcctctct ggggttatct tctcaagaca
    31081 ccaaagccgg gatggagcga tatcgatatt gcgcagagat tgcaaagaaa actgggagtg
    31141 cctatcaagc tcgaaaccga tgtcgctgcc gctggccttg gcgaatggaa atggggagcc
    31201 ggactaggga cgcattgctg tgtatatatc accataggaa cgggaatcgg cgcaggtatt
    31261 ataatcgatg gtattcctat gcgcgcatcc ttccatcctg agattgggca tatgcgcgtt
    31321 ccaagagcca ggacagcaga ctccggacca gatgaattcg agggtctttg tatccaccat
    31381 ggcgattgcc tggaagggct cgccagtggt ccggcaatcg ctgctcgctg gggagaagcg
    31441 ccagaaaaca tgtcgataga acaccctgct tggaagcttg aagccgaata ccttgcatat
    31501 gcgctcacta atattgctct caccgtcgca ccggatcgaa tcatagttgg tggaggaatt
    31561 ggtcttcgcg aaggcctgct cgatttagtc agcgagcgta tgagggagct tctgggcgga
    31621 tatatccagg ccttgaacag caccgcagca ataaggcttt ttctggccag agcagagctc
    31681 gggagcaaag ctggagtgct gggagcagcc gaattggcaa gacaaaccat caatagcgtc
    31741 tcgagtgtct caaatcgttt ataaactttt ggcaaaagca gatcatccaa aaaccaggga
    31801 tagatcctag gcgcgagaaa gcaattcaat aaagaacctt gccttgatta ttatggcctg
    31861 cttctgctat agtacgctcg ttctgaccaa gccgagatag ctcatctggc agagcggcgc
    31921 actcgtaatg cgcaggtgtc gggttcgatt cccgatctcg gctatgatac tatggcatga
    31981 attccattgt tcatagattc ggcagagtcg tttcgaacca ggtcctcgaa aatcctcaac
    32041 gtgcgcgtag tctattatgc acagtttatg cactcagtag ttggaaggat gcatatttca
    32101 gaggtagagg acttagaggg atctatgctc gcatgaattc cgtaataagc aaaaacatga
    32161 gccgctcttt tagggattca tcgcatagcg ctatggtcaa tatctttttt ccatgcgagt
    32221 tactatacgc aatggagatt gtacctgtgt ttccggaagc tttgtcagta tacattgcaa
    32281 attcgggctg tggagagata ttcgcagaat ccgcggaagc tataggcata tccgaaagct
    32341 tgtgctctta tcataagatc atgatcggca cagcatggac aggagtactt cctaaaccac
    32401 tacttatcgc aaatactact ttggcctgcg atgccaatca gctctccttt agattacttg
    32461 ccgatgaata tcatactcct cacatcataa tcgacgtgcc tcatacggtc gataaggagt
    32521 ctgtgcacta tgtatcttcc cagctcaaaa atataacttc ccttcttgag gatctatcgc
    32581 actgcccaat ggccgaatca aagcttcaag acaccatcga tagggctaat cgcaccataa
    32641 aagcataccg aaaatacctg gaccttcgcg cagaaatctc gcttcccatt tccatgaccg
    32701 gcgaattgtt ttctcttgca gccttgcatg ctttcctggg aatgccggag tctgagcaat
    32761 atgccattgc tttagaagaa ttagcgcaca aggcctctct tgatcgaaga aaaaaacgca
    32821 tcctatggct ccatactctt ccaaattggc agcattctat tagagttctt cttgaaaaca
    32881 atgaacattt cgaaatcctg gcaagcgata tgacatatga tacactgatc gatatggacc
    32941 cgaaaaaacc atacgaaagc atggccaagc gccttgtata taatatcaac aatgggggcg
    33001 cacacaggcg tatcgatgca gcaatttcct acgcggaaaa actgcatgcc gatggtgtaa
    33061 ttgtgttcgg tcaatgggga tgcaagcaga ctatgggcat gtcgcaattg gcaaaaataa
    33121 gccttgaagc ccatggcttc cctactctgg tgctggacgg agatggctgc gatccacgca
    33181 atgtagccga tggtcagatg ctaactcgga taagtgcttt tctggaacag ttagaggcaa
    33241 agcgatgatt ggttacacct gcaaatatac gcctatcgag ctcttgtatg ccttaggaga
    33301 gaccacggag ctactcaacc atgaagtgcc ggatttcgaa tatgcagaga aatggactca
    33361 tccaaacctt tgctgccatg caaaggcctt cctgcaacat agctctgagc tggacgagct
    33421 tgtattagta aattgctgcg attccattcg cagggcatat gatgtgttgg ttgcacagca
    33481 agttcatcga tttctgtttt tatttgatct tccgcacata gataagggct gcgcgcaaga
    33541 aaagatcaaa aaggaacttt ttcgccttac tcgactctac acggaacatc atcctgtctc
    33601 ctttgatcga cagcgatttc ttgaagcatg ccgggcttct tctgaggttg ctttcaaggc
    33661 ttcgtttgag tctgcctctg ggactcattc taaactcgat cctgcgactc cgttcatcgc
    33721 gataatagga gcgcgcgtaa gccaagaact tctttccacc atcaaagaag aagtgcccta
    33781 tctagtgatc gatgccacct gcggcggaaa ccggtatctc agccctctgg ctcccggtgc
    33841 cgagagaatg agcttcgaag atcttatgga ttggtatgca aaagagctct tgcattttat
    33901 cccgtgcatg cggatggcaa atatcgagcc gcgtaaagcg ctttgggaaa acgagaatct
    33961 ttgcggaata atctataata ccgtcaagtt ttgcgactat tatagtttcg attatgcgcg
    34021 cgtaaaaaga gaagtgcgcc tgcctatact aaagatcgaa tccgatttta cgcctcaatc
    34081 cgaaggccag ctttccactc gcctcgaagc cttccaggaa aatttgcacc ttagcctcga
    34141 aaagccactg aaaatcaagg caaaacaggt aagtcaacct gccttctttg ggggaatcga
    34201 tagcggctca accacgacaa agatggttat catcgatcgg aacagtaaaa tccttgcttc
    34261 gagcatagta tctactggcg ctaagacaca ggttagcgca cggaaagtcc tacaggaaac
    34321 atgtagacag ctcggcgttg aaccgtgtga cctggcatcg gttttttcta caggctatgg
    34381 gcgaaacaat attgcattcg caacagatag taaaaccgag ataacttgtc atgcaaaggg
    34441 cgcatttttt cttgacccca ctattcgcac aatagtggat ataggaggac aagatagcaa
    34501 gataatccgc atagacaatg aaggcagagt cgtcaatttt gtgatgaatg acaaatgcgc
    34561 cgcaggcact ggccgtttcc tagagatgat ggcccgcacc cttgagctcg atatcgacat
    34621 tatggtgcgc actggcctgg cctggaaaaa cgaccttacg atctcgagta tgtgcactgt
    34681 tttcgcggaa tccgaagttg tctcgctcat cgcggacaat tgctcaattg cagacatagt
    34741 tcacgggctc aataaatcga tcgcttctag aatcacatca atgatcagcc gtactggatg
    34801 ccaggcacca tatatgatga caggaggcgt agccagaaac accggtgtgg ttagagaact
    34861 cgaagttaag ctgggcgaac gactcatcgt ctctgaaaag ccagaactct gtggtgctct
    34921 tggcgccgca ttgtccgctt tggaattgtc tgctttggga tcgaaagaaa tataagcaat
    34981 ctccttgtaa taaaggaata tagcaataaa gagagggaaa aggcatggaa aaagcaaagg
    35041 taggcataat tggcggatcg ggcttgtatg aaatcgaggg agcagaggtg gtggaagaaa
    35101 tcaaagtgga tactccttgg ggatctccct ccgaccttat cacaattgct aagatcggag
    35161 gtgtcaggac agcttttctg cctcgccatg gaaaagggca ttttcttcta cccactgaag
    35221 ttaactcgag agccaatatt gcagccttaa agatgctcgg agtccgtgag atcgtagcat
    35281 tctctgcggt tgggagcctc aaggaggata ttcatccacg agatttcgta ttgccctccc
    35341 agatcataga tagaaccaag gacaggccgg gctccagctt cttcggagaa ggtattgttg
    35401 ctcatttgag ctttgcggac cctttttgca cacgcctttg ctctatcata gagccactaa
    35461 ttctggcgcg aggtttgcgt cttcataccg atgaaacgct catatgcatg gaggggccag
    35521 ccttctcaac aagagcagaa agccagcttt accgttcatg gggcgctggc attatcaata
    35581 tgagcgtgct gcccgaagca aaactggctc gcgaagcagg gatttgttat gcaatgatct
    35641 gtatggcgac agattatgac tgctggaaaa tggaccacgc agatgtgacg gtcgatatgg
    35701 tcgtccagaa cctcaatgca aactcagcac atgccaaggc tttaatactg gatgttgttc
    35761 ctgcattaag catagatact gagtgtagct gccatactgc catgaagggt ggcatcatga
    35821 cagccccatc aaagcgcaat cctgaaacag tgataaaact caagactata ttccctgaac
    35881 tctaggcttt atcggcaatc tgatataggt ggtccggctt gaccctatta gccttgctaa
    35941 ttactctatc ctcatgaaaa tcttgatagc caacgatcat ggcgccgtgg cgctaaagtt
    36001 caatattttg cgatggctcg aaacccaggg tcacgtggtt gtcaatcttg gtgtcgatac
    36061 cgaagatcga gtcgattatc cagatcaagc agccttagcc gtaacggaat atttcaaggg
    36121 tagttatgac ttgggcatag tatgctgcgg tacaggcatt ggcatatcga tagccgccaa
    36181 tagattcaag ggtatacgtt gcgcactcat acacgatagc tataccgctc gcatggccaa
    36241 ggagcacaat aatgccaatt tcctcgcttt tggcggccga gtagattata aagagcctgt
    36301 cgaagaactc ttaagtgtct tcctgcatgc ggaattccgg aaagggcgtc acgctgaaag
    36361 agttgctaag ctcgatacca tttaggctgt ctttaggcta tcttgtagaa aacacccaat
    36421 ttgacaatct cgatgtcctt taggtcagca tctatcagat ggccttgcat tgccatcagt
    36481 gcttcttgtt caccatgcac aaggaaggtt ctctgtaagc gcttcttatc gatacactct
    36541 agccattcta tggtctcttt ccaatcggca tgagcagaaa aagcatcgat ttcttcgata
    36601 tttgcacgta cttggtacca atcccgttga attcgcactt ctttcgcacc atctctgaga
    36661 cgcctgccta atgtcccctc ggccatgtac cccacaatga gcacggtatt cgcagaatca
    36721 ccgagtccat gcatcagatg atgctggatc cgtccaaatt cgcacatacc atctgcagaa
    36781 atgattataa gtggaccttt agcctgattg agagccttag agtcctccac agattgggaa
    36841 aaattgagag ctgcaaaacc aaagggattc ttggcgtggg ccgtaaaaag atcgtaggtt
    36901 tccttatcgt agcattccgg atgtataaca aatatgcttg tagcattgaa agccatgggc
    36961 gaatccaccc atatcggaat atcgggtatc ctaccctgat catgcagaag atgaaggcta
    37021 aagatcagct cctgcgtgcg ctccactgca aaggctggaa ttataatctt gcccctcttc
    37081 ttagcggtcc tgtttacaac atcttcgagg tgtagaaggg aatccgctgt ggattcatgg
    37141 agacgatctc catatgtact gtcgagaata agaaaatcta tcccattgag gcactcgggg
    37201 tccttgatta tgggcttgcc tttgcggcct aagtctccgg aaaacccaag aatagctaca
    37261 ttcccagcct tatcctttgc ggtaattcga gcaaaagccg atcctagaat atgaccggca
    37321 tcgaaaaatt ctatttgtac tccatcagct atagagatag gacgatgata gctgaccgtt
    37381 acaaactgat ccattgcccg gatgacatcg aagttatcat ataaaggctt ccagtcgaat
    37441 tgctcatgtc ttttttctgc ctgcctagaa agataatccg catctctcgc ttgaatatgg
    37501 gcagagtcgg tcatgatcaa gtttgcgaga tctctagtag ccgaagtagc gtagatattg
    37561 ccggtgaaac ctctttttac aaggtatggc acaagcccac aatgatcgta atgcgcatga
    37621 gtaagaacaa gggcatcgat ggatgaagcc tctatatcat ctccgagcaa agccctattc
    37681 ttggcatccg ctttcgcccg ttttccctga aaagcgccgc agtctatgag aattctggta
    37741 gagtcgactt cgagaagatg cttcgaccca gtgacttcct ccgcggctcc aagagaacga
    37801 atattgatgc tcatgttctc actataccct gttgcgaaaa aatccgcaat tcgatagatt
    37861 aatgcagatt catgatcgcc gatacactca cccttagggt tgaaaaactc tcttcaaacg
    37921 gtgagggtat tgctttttcc gaaggaaaag cgatcttctt gccttataca attccaggcg
    37981 agacagtact tgcagaaata atagaggaac attcgggctt tgcgcgagcc aggatcatcg
    38041 acctaaaaga atcctcaccc tatcgtgtgg aaccaccttg tcatcatttt ggcgactgcg
    38101 gtggttgcag tcttcagcat gtcggctatg aacatcaggc attacttaaa aaacaagcag
    38161 ccctggatac ctttttacgg ataggaggct ttgatcccgg agagcttcag atcataatag
    38221 gagaaggcta ccactgtcgc aatcgtgccc agatccatgc ctgcggagat ggtggcctgg
    38281 gctttgcaaa gactggctca cacgagagcg taaggacatc tcattgccct gccctggttc
    38341 cgattataga gcgctggcta gtctccgaaa accgaaaagc ccatccatat cgtgcccttt
    38401 ccgcccttat tggcgacaga cctcgatttg tggtttttgg ccaggagaat cgaatatatc
    38461 tcgaaggaag agacaacttt gcaaaggcaa aggtcgctgg caaagaacta aggttcccgc
    38521 ttgcgggctt ttttcagagc aatatcttca tgctcgaaag attaattgca gagaatgtaa
    38581 tctcattgta cggcaagcga gctctcgacc tttacagtgg tgctggaatg ttctcactat
    38641 tcctggcaga cagattcgaa gctatcgatt gcgtcgagag tgaggctgct tccatcgaag
    38701 ccgcacgcat gaacctcgaa tcagcagctg taaagataga gttttcatgc accacggtgg
    38761 agaattggat agaagctgct cggtctagcc tctcctttga ttgtatcctt gccgatccac
    38821 cgcgaaccgg aatgacgcca aaagtgcgca catggctttc ttttgttgag gctccaatgc
    38881 ttctttatat aagctgcgat cttgcaagcc ttgcacgcga tctgcgagat ctttttcaaa
    38941 aaggctggca actcgagaat atcattctct atgacttcta tcctcagacg ggaaggctcg
    39001 aagcagccgc tcgtcttata aaggaggcta agcatggccc gtaatggtcc ttttaagggc
    39061 agatcgattt cggtggtaaa tgatctatcg ttagatgaac aactctatct ataccgcaag
    39121 gctagagagt taaaagagac tgcgcgaggt ggccacgatg cagaaaaatt ccggatcaat
    39181 gatctggatt atcaagtgta ccttattttt atggaaaatt ccacgcggac tcgcgaatcc
    39241 ttcagaaatg caggcaaatt tctgggcgct cgagtcaacg tctttgacgc cgccacaagc
    39301 tcctttaata aaaacgaatc tataaccgat gccatcaaga tgctctttgg ctattccaat
    39361 gagtcctgtt tcatacttcg cacccctctc gaaggcgtct gcacctggct agatcaggca
    39421 tttgaggaat atgcccatat aaccgaaaaa ctcaagcctt cttttataaa tgccggcgat
    39481 ggcaaacacg agcaccctac ccaagagttc ttagacgaat tctcttttct cgaacaactc
    39541 aattggaaag aagagcatat acacatagcg atggtgggcg acctctatca tggaaggacc
    39601 gtgcattcca aggcggatgg cttaagaata ttcaaagaag tcgaagtaga tctcgtagcg
    39661 ccctcattgt tatccatgcc ttcatattat gtggataaaa tgagggctag tggtttccag
    39721 gtcaatattt tcgaatcgat agatgaatat ctcgcgcagg acaatatagc acccatttgg
    39781 tactttactc gccttcaatt ggagcgaatg ggcgaagccg ttctcgagcg agctccatac
    39841 ttgcgccaat cggtaacttt caagaaagac tatctaggac gattgcccga aaactgccga
    39901 ttctatcacc ctctgccgcg agatcgcaat tatccaacca taccattttt cctcgatgat
    39961 ttgcctctaa atggctggga tggccaatcc atcaatggct attggacgcg cataaccgag
    40021 attgccatgc tctctggccg tattggagga gattttgaag gcgaacatgc aaagccacgc
    40081 gaattcatcg acgactttgt gcatgaagca tcgatccagg agacctctaa acctgagtat
    40141 aaggtaggaa ttaagcctgt tgaagaagga atagtcatag atcatatagc aaggggagct
    40201 tccataggcg agatctggga tactatcgat gccgtaagaa aaatcctcaa gctagatgtg
    40261 cgttccagcc atggtgtcta tcattccaac cgaggcgaag gaatctataa aggcattatt
    40321 tctcttccgg acataataag ctttggtgaa aaagacctga aaaagctcgc agcgatcgct
    40381 ccgggctgta cgctcaatct catacgccat gctcacgtag ccaagaaata tcgcctctct
    40441 atgccgcctc gtatatacaa cttcgacgag atctcctgca agaacgaaaa ctgtatctct
    40501 tctccagcac aacacgaggg tgttcccacc gaattcatac gcaagggtga gactacattc
    40561 gtctgtaagt actgcgaacg cgagcataaa ttcagggata tctgggatat ctgatcagct
    40621 ttacagacta tagatatccc ctctggaaac cgtacttgta tgaatagcag gcatgtaatt
    40681 ttgccaaaat caaatgatct atatcagcat caacaaatgt tcatatcaaa tactcgaggc
    40741 accagagtta gagcgccaaa actcatacat gagaattgcc gcagatacag agacattcaa
    40801 tgattcgaca tgaccctgca taggaataga aacgatatcg tcagctacct ttctaaggat
    40861 ttgcgagact ccgctccctt cattgccaag aataatcacg gattttggag ctataccagc
    40921 gctcgtaagc ggcttgccat ccatgtctgc ggcgtagacc caatagcctg cctttttgag
    40981 agtatcgacc gctgatctga gattgctcaa tcgtaatatg ggaacccagg cgatagcgcc
    41041 agcggatgcc ctagcagctg cattggagat cggagaagca cgccgctgtg gcataataac
    41101 ggcatctaca ccgaaggcgt cagctgatct caggatagca cctagatttt gaggatcttc
    41161 tatatgatcg agaactaaga taaggcttgg ccttggttca gaaactcgat cgcacagagc
    41221 cttcagagaa ggcttcttct taaagacttc ctctagcgcg agaatagctc cgcgttgatc
    41281 ttttgacatt tcatcgagcc tagattcagg cacatattgg attgaaatgt tctgcgatag
    41341 cgcttcatcg agaatctcac gtattcgtgg acctcggcgc tgagagtttt cggttatata
    41401 gaggcagcac tgcgaatttc cagctttgag agtttccaga atagcatgaa atgatgtcag
    41461 atactccaca caccatcctt acgagaaatc gaataccgaa tcctatttct tatgtatcga
    41521 tcatggtata actgtaaatt cctggacgag ctgaattgcg ctctcgatct gtttgacacc
    41581 cggcacaaga ttagccgctg cgacagcact ttccactgta gcgcgactgt tggctactcc
    41641 atgaagcacc acaacgcctc gctcgaccgc tgcctccaaa aaatgaatag ggattttgcg
    41701 attgtaggtg atctctgtga ctatcttagc tccaagcaat agatccctta gctttgcctg
    41761 agcttctttt tctcgatctt cggttataaa ggacactcta aaagcatcga tcgcagaagc
    41821 agcgtggatt ggatcgaagc gatcggttgt tagggtcatg tcatattccg aaggattgta
    41881 ccaatccacg gagaagaagt acttatggaa gcctgctcga tcgtgatcgc tgctctcgat
    41941 tatctgaagc gcatggcgtg aatctatatt ctgcgctttg cgcaccctct cgattcttac
    42001 cgtcttcgaa gcacctatcc tgatgcttat tagattctgg ataccgcgta gaattatatg
    42061 agcccctctg ccaataaaga tgcagttgtt ttccagcgca gtatcataca cggtttgggt
    42121 gagaaaatgc aggtattcgt ccctctgctg agaaagagaa gcccaaaagc ctggattctt
    42181 ctcgtcgtac ctagcctgtt tctcaacatc gattcccatc gctgaaagag cagcttcgat
    42241 atgctccttg tcaacaatct tatatccatt gatctttgaa agctcttgag ctgtttcttc
    42301 gccataagat gccagctcgc gagaaattgc tataaccgcc atattacacc tccctcttaa
    42361 aaatcataat ccaagttagt cctcgagtca aacaaaatac ggaacgtgct ctatcttatg
    42421 aataacattt ggtcgccagc attatatccc cttttataaa tagagtcctt tagatagaat
    42481 atcgagcaat ggagagattt gcgcttcttg ccgcatttgg aaccgcggtg tgttgggctt
    42541 tttcggctat ttttttcgag actgcctcca ggaaagtcgg tgctttggcc gtcaatttct
    42601 ggaaagtggt tttcgcgttt ttgttccttg gaatttccgg gatggtcgag cggaatgtgc
    42661 cttttcctta tgatgcacct ctaagaacat ggatactctt aggcttgtcg ggaatcgttg
    42721 gctttgtaat ttccgacttt tttcttttca atgcatatat actgataggt tcaaggatta
    42781 ctgttatttt ccaatcgctc acgcctattt ttacggctct ctttgccttt atcttcatag
    42841 gcgagcggat gcaagtttat cgactcatcg gcatggcagt gaccgttgcc ggcattctaa
    42901 ttgttgtcac cacccgttct cgccagaaca aaaagaataa taaagaaaaa ctttccgcaa
    42961 aggggctcct atacggcttc ctttcgtcta tttttcaagc aggaggaatg gtgctcagca
    43021 aagcaggctt gggcaactat agtccaatct ccggaacgca gattcgcgca ttcgttgcga
    43081 ttttcggatt tgcaataaac gctctcctca tcggacaggg tagatttgtc tttgttgaga
    43141 tgcccagaat aaaggaggcc cttcgctcaa cattaaaagg ctctattttt ggtccctttc
    43201 tgggcgtggc cctctctctg ttctccatgc aacatacgca ggtaggggct acaagcacac
    43261 ttatggctct gactcccgtg gttatcattg tgccatcggt tctgattctc aagcaaaaag
    43321 tccattatct ggaagttctc ggcgcggctg ttgcggtagt aggagccgct ctctttttct
    43381 tattatagca ctaatttctg cttcattgtc atacacaagg ttcgtatcag tatggaatgg
    43441 atctgtcgaa gactgatttt gattactgca tttctcattt cgatatttgg tagtatactt
    43501 acgatagaat ttgatttatg cgctttgcat tcctataaga aatcgatata tagatccaaa
    43561 ataatggggg aagaaatgcg cacagtcaaa aaggagcaat atgaaagcgc ttgtcaagag
    43621 aaacgctgaa cccggactct ggatggaaga agtccctata cccaatattg gagataacga
    43681 tctgctgata aaggtcaaga aaacagcaat ctgtggaact gatgtgcata tatggaaatg
    43741 gaatacttgg gctcagcgta caatacatat tgggcagatt gtaggtcatg aatttgtggg
    43801 tgagattgta gataagggta gatccgtaca agactataaa atcggagaac gcgtttccgc
    43861 cgagggccat atatggtgcg gtacctgccg cgcatgtcgc gctggtaagc atcatctctg
    43921 ccccaatacg attggaatcg gagtaaatcg agatggttgc tttgcggaat atgtggctgt
    43981 cccggcacag aacgcatggc atgtgcatga agccattcaa gatgatattg cggctatttt
    44041 tgatcccttc ggcaacgcta ctcatactgc actctctttc gatatgactg gtgaagatgt
    44101 attgatcact ggagcaggcc ccataggctg catggccgct gctatagcgc gccatgttgg
    44161 agccagaaac gtagtggtga ccgatgtaaa tccatggcgg ctcgagctgg caaaaaaact
    44221 aggcgcaacg cgcgttgtaa atgtcgataa ggaagactta aaagaagtac agaaaagtct
    44281 tggaatggtc ggcggtttcg atatcggcat ggaaatgtca ggcaacccca aagccctcga
    44341 gcaaatgatc gaaaacatgt ataatggagg acgaatggct ctgctgggac ttcttccgga
    44401 tggagctggt atcgaatggt cgagagtcat attcaagggg cttttcatta aaggtgtata
    44461 cggacgagaa atctttgaaa cctggtacaa aatgcaaacc atgctgctat ctggtctgga
    44521 tataagccct gtaataaccc atagatttcg cttcgacgac ttcgagaaag gctttgaggc
    44581 tatgctttcc ggagagtccg gaaaagtaat cctgaatctg gaatgaagaa gtccttgatt
    44641 gctcaattat tcgcccatga ttgaacatac tcttctctat aatttccgta ttagcgaaga
    44701 tgcggcctca gcattggata aaattccacg ctgtatcgaa gaagcaaatc ccatttcctt
    44761 ccagcatcgc gcagagcttc gctacagcat tcggagtctt tggcgactac tgacaatggg
    44821 acgagcccag cgcgcaagcg actatctcaa caatcctacc cttttttcgg cctatttgcg
    44881 ctatttcatg ccctggaacc ttgtacgact cgtttccctt ttgacggatc ttcctgttga
    44941 acttaagcca aatgctacta taacagatat gggttcgggc ccgctgacct tgctcttatc
    45001 gctctattgc tccaagcctg aattgagaaa gctccctctg acgatcatct gtgtggatcg
    45061 agcacctcgc atgatggaga ctggaaaaac cattctggaa atactcgccg cggaacattc
    45121 tggagaattg cctccatgga aaatcgaact gagacacctg aggttcgggg aacctatcag
    45181 ggaaaaggcc gacctcttct gtgcaataaa tgtcttcaat gaattcttct ggaaaagcaa
    45241 agaaacgctt ttcgaaaacg ctgacgctct atatcgatct attatgcaat actgcaagaa
    45301 tgatggctca ttgctgatcc tggaacctgg cgagcctaga tgcggagggt ttttgagtgc
    45361 tctgcgcgcc gttgctattc gggataattg cgagattttg gctccttgtc cacacacctc
    45421 ggcttgtccc atgcctggaa ttttcaagag tggacaagaa tatctaatta aagaaaatcc
    45481 caaaaacata atcaaaaaag gaatccctcg ttttgattct attgagagca aagcaaatgt
    45541 tgcacgacaa aaaagtgaat atataatcaa gcagctcccc gacgagtcca gcaatcatag
    45601 aaataggttc ttgacgcctg tccgaatgcc cgctccacga gtaaaatatc cttggtgtca
    45661 tttctccatg cccacaagct atgctcctaa atggctttat aggctatcac aagaagccgg
    45721 gctggccaaa gataaattgt cttttagcta cctttacgta aagcgatgcc catcctcgat
    45781 tttgccctcg gatcagaata agaagcaaaa gagtcgttac cctggaaacc tgcccttgcc
    45841 gacaggggca aatgagggat acgatgaact cgcctgccga attgtgtccg aaccaattgt
    45901 gctttctcat tcgagagtcg gtaaatatgc ttgctctcac ttaggatata ctctgatttc
    45961 gaccggacaa tctgaggcat tgccagagtc tggtgctctc ttgcgcatag caacaaagat
    46021 acctgcaagc accagttcat ccaaaaaacg caaaaaagaa atggaatatg atcaaaagac
    46081 aggcgctatt cttatatcct acgaatcgcc taaaaagttt tctggccgaa aataagccat
    46141 taactttctg cttgcggacg tgaaagagcc tttcagccgc tttattcaac ttccggcaca
    46201 atggttcaat ttggaccagg tcttattatg aggcatcggt atcgtttcgg ttagactttc
    46261 gatgaggcaa tgcgtcctat tgacacaaaa caagggtgcc tatatagtac catgccgtca
    46321 gccatgacaa gggggcgtag cgaagctggt tatcgcgccg gcctgtcaag ccggaga
//
LOCUS       DAOMZL010000004        44532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_599_length_44532_cov_16.872833, whole genome shotgun sequence.
ACCESSION   DAOMZL010000004 DAOMZL010000000
VERSION     DAOMZL010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 44532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44532
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_599_length_44532_cov_16.872833"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLQ30_00855"
     CDS             complement(<1..446)
                     /locus_tag="PLQ30_00855"
                     /inference="COORDINATES: protein motif:HMM:NF019656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HPW01023.1"
                     /translation="MPELEKYDHGPTKIERNNVIERLEHGFAQNLLDIDVYEKRMEIA
                     VNTASKRELLSLIEDLPASAETNKQFTSLDGILLNHGEIKDYDVTLAFLSGATRKGLW
                     YPPKRMKVVAIMGGVDLDYSQAQIPPGVSTIDVFALMGGVDIFVPEG"
     gene            644..1624
                     /locus_tag="PLQ30_00860"
     CDS             644..1624
                     /locus_tag="PLQ30_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01024.1"
                     /translation="MSNLCQEKDGNLHDFELHPRGRGGAAIIYPVISRRAGGLSLGIN
                     LFPERKICSYDCPYCEVMPFSNPGASLKAGDVSRAIECFFDEEWPELSKTLELKDISI
                     SGNGEPTLSPFLEEALYAARQAIDTKALVKDCATPSGQHSIPSIVLITNSTGFLREDI
                     TELLAGFYKRYPFEIWAKLDGGKEELHALLSRSRFPFAEIVRGIVDFSCSVPVTLQTM
                     VIYDNRTGNIYFDCQSYAVTIREILNGGGQLRAIQIYTTARVPVESWVAPVSDERLVI
                     FARQLSSILNRPQRKKATGKEEEALQNQVYSIPILCYGSRGKLDWNHANR"
     gene            1611..2462
                     /locus_tag="PLQ30_00865"
     CDS             1611..2462
                     /locus_tag="PLQ30_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPW01025.1"
                     /translation="MPTADLHTHSTASDGELTPAALVEKARALGLSTLALTDHDNTLG
                     VAEARAAARLVNLEFIPGIEIEIDFEPGEFHLLGYGIDEAAPPLVEAVERLSHARLER
                     NLAILDRIAKANLPLDLDSIEAFSKTSYLGRPHLADLFVEAHCARTRQEAFDRYLGKG
                     KPFYIKKACLALEEALSVIDAAGGVSVLAHPYSLFVSKDVLGTIFSQLKAKGVRGIEA
                     YHPTAKYGQCVILERMAHERGMFVTAGSDFHGKMRPECGLGRTSGNKPIDIRFRDELV
                     AALESRI"
     gene            complement(2596..3696)
                     /locus_tag="PLQ30_00870"
     CDS             complement(2596..3696)
                     /locus_tag="PLQ30_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPW01026.1"
                     /translation="MKTTNPRKISFLILFLALLLLVGRLFYPFLTVILWSGLLYVFLE
                     PIHKRLRGPNSIKGRKTMRSRLSALFLSIFGVIIILIPLAFLAFKTIQQLADLLKLSI
                     HFVEENIDKLRIDPQNQIGSAIQSLLGDLYDVSTFDPLRELQSFLASGVNQLIKISTS
                     LIMNISKFVVTICFMVFTLYYLLMDASSLGDTFVSMMPIAPQYTRLFMSKLRETGRQL
                     VIGYFLVSLFQGFMMFILSIIFGFKNNLVLAVLTAISSFVPLLGTTVVWAPMGIYLAV
                     NGQIIKAIVFVACASVIVATLDNFIRPIVLGEQIKIHPLLLFFSITGGLAVFGFNGLI
                     LGPVILILFVAAGDLYRALNEESELSDNKSEK"
     gene            3895..4587
                     /gene="rsmI"
                     /locus_tag="PLQ30_00875"
     CDS             3895..4587
                     /gene="rsmI"
                     /locus_tag="PLQ30_00875"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPW01027.1"
                     /translation="MIATPIGNLEDISLRALETLKSVDIIACEDTRHTLKLLSRYEIR
                     KPLISCHSYNEQQAAKRVIALLSEGKDVAYCSDAGTPGFSDPGALLVRMARSAGYKIV
                     PIPGPSAFACLISASGLSGKTFLFDGFLSPKPGKRRTRLKELLDRGESFVLYESPFRI
                     GKLLADIASIDPERRLCIGRELTKIHEEIIEGKAEELVQRFPAESAKGEFCIIVHGIK
                     PANSVNDKEDES"
     gene            4584..5399
                     /locus_tag="PLQ30_00880"
     CDS             4584..5399
                     /locus_tag="PLQ30_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HPW01028.1"
                     /translation="MMDISHFRNLHGRARLRKAVLLLERLEREWRKADPLIIPYEDLV
                     FVKQIAKILADSEETTLECVKAASDFLGSLSGLDDILDYYGFLQTENKFRHRLMILSG
                     QGVADWDFIAVPRSSAGHATKRAGMRVYLEDLRSPFNVGSIFRTAEALGFEEIIISPE
                     CADPMHPRAIRSAMGTIERMKWRRAELSELDCIQGVFALELGGIAIDKFSFPFPGLMV
                     VGSEELGVSNKALQYCRLGCIEIPLVGTKASLNVATAFGIAAYSWYYSGNMQA"
     gene            5467..6357
                     /locus_tag="PLQ30_00885"
     CDS             5467..6357
                     /locus_tag="PLQ30_00885"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPW01029.1"
                     /translation="MSLNPFMKKLGLSSSDRAVIVHVDDVGMCLSSIEAFFELYDFGT
                     VTSGALMAPCPWFPEVARRCAARPDVDVGVHATLTSEWKGYRWGPVSTRDPDSGLLDA
                     EGYFPMTSEEVQSGAKPEAAMKEIAAQVAMVYAAGIEPTHIDTHMFSVAHEKLFDAYI
                     SQALEHRIPAMLPKPDIVGFESIVPDCETRKYLAGYLKELGEKGMPLMDGILAMPLDE
                     PRDQTETVKRLLGSITEGITHFVLHASTDSHELRAITPDWESRVWNYRTMHSDTMKKI
                     IESEGIVLIGYRNLRDAMRK"
     gene            6494..7615
                     /gene="ugpC"
                     /locus_tag="PLQ30_00890"
     CDS             6494..7615
                     /gene="ugpC"
                     /locus_tag="PLQ30_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HPW01030.1"
                     /translation="MAKVELKNIGKVYEGNVRAVSDANINIHDKEFVVFVGPSGCGKT
                     TTLRMIAGLEDITEGEIYIDGRLVNDVPPKDRDIAMVFQNYALYPHMTVYDNMAFGLK
                     IRKLPKEEIDNRVKEAARILDIEKLLDRKPKQLSGGQRQRVAVGRAIVRKPKVFLFDE
                     PLSNLDAKLRVQMRAELIELHDRLQSTMIYVTHDQVEAMTMGDKIVVMKDGLIQQIGS
                     PLYLYNNPINKFVAGFIGSPPMNFMSVNVIEEGGQVLIDEGSFKLAPDAKHAELLKAY
                     VGKSVYFGIRPEDLPVADAVHRGQKFNAKVTVVEPLGSEIHLQATTPTQGMVSRIPPH
                     HLYKHGDTIIFAPVMAKAIYFDKETEKSILPVKWNEQEA"
     gene            complement(7891..9189)
                     /locus_tag="PLQ30_00895"
     CDS             complement(7891..9189)
                     /locus_tag="PLQ30_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW01031.1"
                     /translation="MSEKKGFKPYLGITFLIGFGFFTMGLMDPLYDTYVPIFLSRYLS
                     KMSLVGFFMTLDNVLAIFLIPLVSAWSDNTYTKLGRRMPYILVLLPLTAFLFGAIPYA
                     TEVSLVALLVTLILLNVTKQSVRGPVVALMPDTIPADYRSEANGVINTMGGIASIVGT
                     IGLARLMDLDITLPILGPTKDKLPFPLGGLFVILAVILLFIFIREKQPDAKETQEQKV
                     PILESFRNVAAQKDKSALYILIAIFLWFLGYQGVLPFIGKYSVDVLRTSSGMAALAAG
                     MVGIAYAIFAIPSGYVAHRFGRKKTIRTCLLVISILTLLLFFHKWITAGLSNSLRLGS
                     FWAIMFLFGIFWVSIITNSFPMLWQMAEWGTIGIYTGLYYTASQAAAILGPVLTGVII
                     DIFGYQGIFLFCTICMICARAIMGKVTKGEPSEIQNATQK"
     gene            complement(9222..10478)
                     /locus_tag="PLQ30_00900"
     CDS             complement(9222..10478)
                     /locus_tag="PLQ30_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HPW01032.1"
                     /translation="MIQQKIPSIPLASFQDLEKEGRRARYDSLVSRLPLGEDSHSRAL
                     HYYSSPGRTELGGNHTDHNNGCVLAASVDLDMLALARPRSDSMVTLLSEGYSPIRVDL
                     RDTAPRIEEKGSPASIIRGLAAWLTHQGLQIGPGFDIAMDSRVPAGSGLSSSAAFELL
                     IASIFDDMGKYDLTPTDWAIAGQWAENEYFGKPCGLMDQIACAEGGIVFIDFADSRRP
                     KIDRLIYDFESNGLILAIVNTGSNHEDLTREYADIPKEMKCVAAILGRTALRGTEKQD
                     LLNRLSDIRIKCGDRALLRAWHFVYENYRPARMVEALKRNDKSAYLSIVNESGRSSWH
                     YLQNIHAGNPREQSLSIALMLSEDLLSPEGAWRVHGGGFAGTIQVYVPQSRFPEFVER
                     MEAVFGKGSVQRINIRPFGVCKVLMN"
     gene            10805..12106
                     /locus_tag="PLQ30_00905"
     CDS             10805..12106
                     /locus_tag="PLQ30_00905"
                     /inference="COORDINATES: protein motif:HMM:NF006388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW01033.1"
                     /translation="MNQLAEELNAVLAGTVAERLLSEMGRRMYFPRGIITQSAEAGEK
                     AYRFNATIGMAYEHGQPMMLDALRAELPGLTPTEAVAYASTGGIAELRKVWKESLYRK
                     NPSLDAKKISLPAVVPGLTAAVSYICDMFVEPGDTVILPDLHWPNYRLIVEERKSAAG
                     ITYPLFAGKGYNIEGLLEGVRQAGQRRGKAIIILNFPNNPTGYSLTLKEADSITAGLL
                     EIAKQGTDILVIADDAYFGLQYEDGLLCESMFARLADIHPNILAIKADGPTKEDYVWG
                     FRVGFVTFGAAGFGDEHYEALAKKLLGVIRSSVSSSSGLSQNLLLKALKYQGYEAQKA
                     RYRDILKGRYKAMKNKLAEMTLPDGLIPMPFNSGYFMSFRCDGFSAEALRKKLLNEKG
                     IGTVSLMDRYLRVAFSSVEESDISDLCDAIAQAVVELCQQG"
     gene            complement(12136..13353)
                     /locus_tag="PLQ30_00910"
     CDS             complement(12136..13353)
                     /locus_tag="PLQ30_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HPW01034.1"
                     /translation="MKNAYSFPLENFINRQKFERIKEFSRDKETPFLIIDLDIIKKNF
                     EDLRAYLPWATIYYAVKANPDDAVISLLRDLGSNFDVASRYELDQLLSLGISPDRMSF
                     GNTIKKEKDIAYFYEKGVRLFVTDSINDVDKLSRAAPGSKVFFRLLTEGLGADWPLSK
                     KFGSHPDLARQLIKTAIRFGLEPYGISFHPGSQQRDVGQWSSALTIAAQLFNWARHDL
                     KIDLKMINMGGGFPANYLDPTDPLQQYAEDIKRFLDNSFGLVWPEKIIIEPGRSMAGD
                     AGVIVTEIVNIAKKSVHDRYPWVFLDIGKFGGLIETLGESIKYPIYFEGQGSAEEIIL
                     AGPTCDSMDILYERTPYFMPSSAKVGDKVYILTTGAYTRSYSSIYFNGFPPLKSYVLQ
                     SAQDQSSMTAKEI"
     gene            13453..14226
                     /locus_tag="PLQ30_00915"
     CDS             13453..14226
                     /locus_tag="PLQ30_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01035.1"
                     /translation="MITFFIKKAFFDGWDNLYLLAALNGIFLGLLLLFLVVPIALAFP
                     IWLSIIIAGIGIFLLSIWDVVVTDTMYGIADGKSVHFADIKVAIKGSIKLGLLLGVVN
                     ILIAVAIMVAIPFYLGQKAVLGIFIGGLLFWMMVLAMLILQYVPAVFARERGTPRQAF
                     KTAIYLFIDNPGFSIFLFFWRILTFAISIFVVLLAPGPAGSSLASAVAVRLRMKKYSY
                     LNENPGANRRHIPWDDLLQEEKERVGKRTLKGMIFPWKD"
     gene            14259..15485
                     /locus_tag="PLQ30_00920"
     CDS             14259..15485
                     /locus_tag="PLQ30_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW01036.1"
                     /translation="MKPPSRDKSRGNQKKGRANQDNRQLLPEAFEERKKQALSSILYI
                     RFEGADAQSRLPSGIDASIPYPVQLQAPAVKLDPSSITIESLLAGMLRVLAWDSENEN
                     ANKYRKYIKMSRPELFEELIVAGIQKAEEKDWIVAEEVFFAASGLEPERPEPYINLAL
                     MHEEHAKRLFDSGNEDEAEKEDDLAYKYYQYLLGFKDVFAPAYYHAAFFFLRKHNYDK
                     AVSLLTSYIGMNDDEQRTRRAKLILEKLGGMGYLDTLFKEAYDFIQMGQEEKGLEKAM
                     QFVEKYPDVWNGWFLVGWANRRLGRWSEGAQAFHTALEKGADGADVYNELSICEIEAG
                     DLEAARSHLERALRIEPENEKIIVNLGALAFRQGHIDEAEGFFRTSIELDPDDRIVKE
                     WIKKIQALRERKGTGA"
     gene            15502..16554
                     /locus_tag="PLQ30_00925"
     CDS             15502..16554
                     /locus_tag="PLQ30_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01037.1"
                     /translation="MTIRSEFRSSEPPAAEELEAAIDAGIAANLHVSAELRPSDPANS
                     LPGGLIVLPPDKPCILVPDLHARPSFLHALLLTEFPGLSEPLLSVLSKNNATLLFLGD
                     IPHAEGEEAARRWISSYDKALIARDVRALLSPEMDEEMGLSLTSLLKVIELQCGFPNS
                     VFCLKGNHDNMTNAGDHGDFPFYKYADEGKMGAIWLSFRYGPFIMHKLREYELSLAIA
                     AENLNFCASHAEPAQEITRDSIISYRSNSRIVQALIWTSNDEAEEGSVRKSLKSILGS
                     EDRARHSVWFSGHRPVSGHFALRAEGQLVQIHNPALWQVVLVDAESRRADFFTILPDQ
                     PKPKKAASLVLDPLAR"
     gene            16573..17406
                     /locus_tag="PLQ30_00930"
     CDS             16573..17406
                     /locus_tag="PLQ30_00930"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HPW01038.1"
                     /translation="MAVRKKKRNQAKTGCLIWLGAFLILLILFLVKFEDIRTAVEKTG
                     FIDALNHAVSKQDSEPQQKPQETNPEEPAPAPTLPSAAPPAEEPQIQSPILPKETTTT
                     TKAPKEVELPSAPLSESEPIASSPGASISKLSKEEQKTRTAVLYFVYIGDDGSISSQK
                     VKRTLAISDSPLQDTLESLLKGPTESELRANLLSLIPADTKLRSVNIKGGTAILDFNE
                     AFAYNRYGKEGYLAQLKQIVYTLTEFQNINDVQFLIEGKSRAFLSEGIVLDKPWSRTS
                     F"
     gene            17492..18571
                     /gene="gcvT"
                     /locus_tag="PLQ30_00935"
     CDS             17492..18571
                     /gene="gcvT"
                     /locus_tag="PLQ30_00935"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPW01039.1"
                     /translation="MHDWHVSHGAKMAPFAGYDMPINYPTGAIEEHLITRRGVGLFDI
                     DHMGQFEITGDAAESFVSHMISARVSDLVAPMARYSLLLNEKGGIIDDLFVYRLQNSW
                     WIVVNASNRAVDLEWFRSHVPSNVSVVDRSDDTYMIAVQGPRAIELLDKISNTPVSPI
                     PRFCWANISIRGIPILFGRTGYTGEDGGELFFPAEKAIELWEYLLECGESYGIETRPI
                     GLAARDSLRFEAGLPLHGHEIGVDINPIEAGFRWACDFEKEFIGKDALLSIIEQGVRR
                     KLVGLEVFGGVAREGYEVATPDGDIIGRCVAGMYCPTVKKYAANAFVIPDYAKIGTKL
                     SVIIRGQPRSAEVIKRPFYVPVYRR"
     gene            18629..19012
                     /gene="gcvH"
                     /locus_tag="PLQ30_00940"
     CDS             18629..19012
                     /gene="gcvH"
                     /locus_tag="PLQ30_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPW01040.1"
                     /translation="MAIDKNARYLESHEYAKPEGELMVIGISDHAQAELGDVVFIEFP
                     AVGKELDKGVVFGTIESVKAASDLYMPISGKVVEVNEEVKNDPSLVNKDCYGSGWLIK
                     VAPSKLSEFESLLDAESYGASIGEV"
     gene            19017..20336
                     /gene="gcvPA"
                     /locus_tag="PLQ30_00945"
     CDS             19017..20336
                     /gene="gcvPA"
                     /locus_tag="PLQ30_00945"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HPW01041.1"
                     /translation="MSFVPHTDLERAEMLKAIGIPSIEDLFSDIPEHLRIEDLALPES
                     ISEIEAIDQLETLASRNVQTKDMDWFLGAGAYYSYIPAVVPALAGRGEFLTAYTPYQP
                     EVSQGTLQAIFEYQSMTAKLLGMSVVNASHYDGATALAEAVLMAWKARENRNRIFLPK
                     DLHPEYSQVISTYCTSFDIEFIRYEGDPGQVAIDEDTAAIVVAYPGFSGQFYPVSNVS
                     DKAHEKGALCIVQADPLMCAIFKSPGEQGADIVTAEGQCLGNPLNFGGPYLGMMGTTD
                     ALIRRMPGRIVGQTKDKFGKRGFVLTLSTREQHIRREKAVSNICSNQGLSMLQTCIYL
                     ATLGKNGLQKVARLSYDKAHYAAQLIDSIPGYHVVPKLFFREFLVETPDAAAKIVEAL
                     NKKGIVPGLALSRYYPERKNELLICVTEMNTKAQIEKLAVALKEAAK"
     gene            20333..21775
                     /gene="gcvPB"
                     /locus_tag="PLQ30_00950"
     CDS             20333..21775
                     /gene="gcvPB"
                     /locus_tag="PLQ30_00950"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPW01042.1"
                     /translation="MNEINMRDEPLVYELSKEGRIGCSLPRSDVPSTGMPPELARTEL
                     DLPELDELTVVRHFTRLSQKNFSIDTEFYPLGSCTMKYNPKANEVVASNPGWKNLHPL
                     AEEQYAQGAIELLWRLQEGLREVGGFAAASLQPAAGAHGELSGVLMIHAALKELGETE
                     RKKMLIPDSAHGTNPASCTMAGFTTQTIPSGPDGSIDMEALRAALDDTTAGIMITNPN
                     TLGLFERNIEDICRLVHEAGGFVYGDGANMNALTGVFKPGRSGIDVMHFNLHKTFSTP
                     HGGGGPGAGMVAANETLAPYLPGPIAIKEASGYRLKMPAKSIGRVKAFHGNFGVLVRA
                     YCYLLMTGADGLRQVAENAVLNANYLKSLLENDYPVPYKRHCMHEFVAKGTIAADVHT
                     LDIAKRLIDYGFHPPTIYFPLIVQEALMIEPTETESKETLDLFAEAMKKIAEEARTDP
                     ELLHDAPHIAPVARLDEVAAARHPILCYRS"
     gene            complement(21910..24180)
                     /locus_tag="PLQ30_00955"
     CDS             complement(21910..24180)
                     /locus_tag="PLQ30_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01043.1"
                     /translation="MTERRIFLSLGAVIFITAFLFIACPSGIPKGNGYVYIRCVLPGN
                     LTEGNTRKTQSRLIGPESGWNIASYRLTFTSENGEVITKSIGKTGAELSLKAGNWSLL
                     AEGLTADGAIIVKKETQVLAQAGKRANIQIMMHLAQGKGSLQLALSPNQPLPQDWKYS
                     LSLEYRGLPGDPTFQGPAPLSTDVPATQMDYQVNELESGNYSLAIQVKDTSSTSIAGC
                     ITTVLILPEQTSRGSCSISLPDPSATISLSTPKLELSADAAISVERYLNRNKPISVPF
                     VIAQGEADLDVQWYQNGTIIEETQAEADQSLPGYKTFFSPDKQPDTQMRISMEVLLSE
                     GNSGISQAFSHSSSLLTGPSSSEIEWIQSIDYKAALNPSLHNSIYPENAGTGNLHEAK
                     SVATSPTGLIAVAGLDQASALHLFYSPYGEEATLDGNNRQIPSSIGWIRLWRDRIFIQ
                     KSEKNPDIASISMDGSMIAIAATAGKWIRVYRLENSGSISRKIDIISGENGAPTFENI
                     RAMRFTTDSTRLFILANSSGKLLVLDIEKLFRGEPCCEQEFSITGWLNRTSEGSQQNP
                     SETEHSSLGMQDMILLDDGWIAACSSTIKMVIFIRYSSSDCSFSEPIIFTSGSNSASL
                     GDPKSIAFDEGNERCYVIGAARKLHLFEKSDPLSAYSLSMTYSLSSDLNEANSLALVK
                     KNSGGAYLVAGGNHGLGIISLDTDGHPICQGSLNSQEDNLYGIKSINNVTVLGESVIS
                     AGGSSGIVAMFDILGL"
     assembly_gap    24263..24362
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            24491..25963
                     /gene="glgA"
                     /locus_tag="PLQ30_00960"
     CDS             24491..25963
                     /gene="glgA"
                     /locus_tag="PLQ30_00960"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HPW01044.1"
                     /translation="MKILFISSELTPFAKSGGLGDAVSSLAAALSKLGHDVRVLLPRY
                     YFISKNILRRINGLLEIDSARGRHRAFLYTTNLPNSQVKVYFLECESLYGRNGIYGYS
                     SNQEFPDNPERFSVLSRSAFAVCRNLGWIPDIMQAHDWPTAPVPVYLNTIEKNTEFSG
                     TAGVLTIHNLGYQGIYGRDHFAELGLGWEYYYGAGFEYYGNINFLKAGIVSAECIATV
                     SPTYAMEIQTPSGGFGLDSLLRQRSRDLVGILNGIDTEIWNPSKDPYLPAHYSVEDIA
                     GKAICKESLQRELGLPIEPEVPLIGMVTRLVEQKGISEVFGRTYGCMRKILETIHVQV
                     AVLGSGDAWAEEAIVNFSAQYPQFKGVIGYNERLAHLIEAGSDFFLMPSRYEPCGLNQ
                     MYSLVYGTLPIVHRTGGLADTVVNYNQNLGDGTGFMFDDLTPQAVYDTVGWAMWAWYN
                     KPEHIKTMRERAMKQDFSWNRSAEEYIRLYQHALAIRKAR"
     gene            26009..27523
                     /locus_tag="PLQ30_00965"
     CDS             26009..27523
                     /locus_tag="PLQ30_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01045.1"
                     /translation="MSETKILDYNKQEVRDQLLRALRSHTKESTSADLARATGLPLAQ
                     INAEMPAISDEYRGRIRVSERGDLLYSFPNGFKSRYKGFGPSLRRLWKTITRGAVKTG
                     KLLFKVWILVTLFGYFFLFLALALIALFASVMMRAGGSSDSNDRRGGGGLGGLWLTTN
                     LFDSMIRLWFYSELFKGPETRYRGTRAKAQRKPLYKAVFSHVFGDGDPNAEWDTILKK
                     AFVAFVQTHKGVITLPEFMAISGLKPEEAEDCITRYMVEFEGSPEVTDNGALYFFFPS
                     LLAKATTVSGAAASTFPIKKLHRFSSNPEKLDRTFRWVNIFNLVFGSYYLWNAINLGT
                     AIIIQTPEGQGLKGGLSFLYSYTVYLFSVLGANQPAVLVGWVLGVAPLAFSLLFFGIP
                     IVRSQRIKRENETIKRENMRRVLYSSVIADPNYFDPQSLQLPANEVRPEKADAVLEET
                     QHLAAWSRGEISPEGKWSFGDIEFIEREAERVRASIEERDYSPQKTVFDTDSPI"
     gene            27547..28563
                     /locus_tag="PLQ30_00970"
     CDS             27547..28563
                     /locus_tag="PLQ30_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPW01046.1"
                     /translation="MTRARNSTAMRGFIAAMISAISFSTLGIFAELLYSHGFSVLCVL
                     AWRFLVAALFLWLVVYIREWFQSKDIPAEKDATKKIADHEQRRKYIPRVFLLGLVGFS
                     PQAGLFLFTVKILDPGIASLLMYLYPSFVFLILFVWRHQRPKRIQILALALSFLGCIL
                     TFWKAGSYPFMGLALGILVAVAYAVYLVIVERVLVGVDSLWATAIIMTAAALVYLCLA
                     LLQGTFIIPSSTEQFIIVLCIAIIASVLPIVALFVAMKNLGARDTSIISTIEPVFTNI
                     FSILLFGENLTIQRILGGVSILAGVLILNRWGYASHKKDSAADLSGPSSPLSKSPGTE
                     EGKL"
     gene            complement(28479..29345)
                     /locus_tag="PLQ30_00975"
     CDS             complement(28479..29345)
                     /locus_tag="PLQ30_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01047.1"
                     /translation="MKSRIRGEPSGMRDKASEESILQPNTDSLLSHLPEINRMRGWRM
                     YARNGSRILSLWSDYGRTLLGYSPRGLVSIAKSSMDRGLFSAVPGIWNSRIEKMLHRL
                     LPEYRNIKILGSQEKAIKLLQNLEGLASSTPSIGTCKFLFDKYLKRDDELSDIISIVL
                     PIPDFLSIGILASKMSSSPVIEQELIEGIKGMTSIYALSKMQSEEEGVISGGLEPVWE
                     SFDRYGGVLFSRSGPWLFPNYPRENHEEVFLYCLEHGLLISPDYNFPSSVPGDFDKGE
                     LGPLRSAAESFL"
     gene            complement(29342..31534)
                     /locus_tag="PLQ30_00980"
     CDS             complement(29342..31534)
                     /locus_tag="PLQ30_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPW01048.1"
                     /translation="MILGQIPEYLEGLNPEQLEAVCYEGNRLLILAGAGTGKTRVITT
                     KIAHLVKQSRFLPESILAVTFTNKAANEMRERAMAIEPSCERAIIRTFHSFGAWFMRR
                     NANAFDLRSEFSIYDEDDSAELVHSIFPAFTKRDCGDYAFLIAKAKDYCLSPDSPNLD
                     FISRHPEFRRIYSAYEDRLRSTGNVDFGDLILMPALLLEKDETIRNRTRQRFRVILVD
                     EYQDSNIAQFRLLQQLAGPDSMLCVVGDDDQSIYRFRGAEVKNILNFPKVFADTRIIR
                     LERNYRSHQAILDLAHSIVVHNENRLGKNLLATRPGGNKPKIAFLDDQDQEIEFCAHI
                     ARQHVKNEGEWKDIAILYRTNAQSLGFERLFPQFDIPYRIVGAVRFYEREEIKDMLAF
                     LAILANPRDEISFKRVVNKPSRGIGASSLDKIISFAFHNGLDLIEASRQSIASISRKG
                     SEGLKEFLSLIEEAKASLHRDLADQKNPGDLGSLISDFAQKSGLLEFYASKDRISSTH
                     KKENIEELVNAASDHPLSSEGLLEFLDDVMLDQSRQKNQDNADAVTLITMHNTKGLEF
                     PIVIVTGLEQGLFPRNDEEGDDIEEQRRLFYVSVTRAKNQLYLTSCRWRRIHGKLFET
                     MPSQFLSEIDPSLYKMWGDSRNKMRAVPSQYRSSSTVAVSSQSDTSTKKSEWTAGMSV
                     YHDEYGNGTVIKVTPSQSAGPLVIVQFETGKTAQFFPKYTKKLERIKN"
     gene            complement(31531..32862)
                     /gene="rimO"
                     /locus_tag="PLQ30_00985"
     CDS             complement(31531..32862)
                     /gene="rimO"
                     /locus_tag="PLQ30_00985"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPW01049.1"
                     /translation="MPTFFVDQHGCAKNQVDGEEISSRLINAGFLAASSPDEADAIII
                     NTCGFIEDAKKESIAAIVEAKTRLPKKKVIAIGCLSQRYPEELFNGLPEADGVLGNGN
                     LSIIPEAVTKILRGERIVLATAQNKKMPSPYYPRIVFFDYPGTAHIKITEGCSNYCSY
                     CAIPLIRGELRSRSIEDIVLEARNLIAQGMFELVLIGQDLGNYGKDLSGRCLLMDLME
                     ALSNIEADFRIRFLYIHPDHFPSEILKIIAHDSRFSPYFDLPFQHASTPILQKMNRHG
                     SATEYLNLIYRIRSELPMSMIRSTFLVGFPGETEEDFAALLNFQKTAEIDWVGVFTYS
                     LEENTPAYDMKARIPKSIARKRKAILENEQIAITANRLQRFIGVSDTFIIEEAFDHDE
                     LCFGRGWMQAPDIDGVTIINASLKPGTIAKANIVSVNGVDFNAELEYTTTP"
     gene            complement(32927..34288)
                     /locus_tag="PLQ30_00990"
     CDS             complement(32927..34288)
                     /locus_tag="PLQ30_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW01050.1"
                     /translation="MGKIGDLFREARLGKGLTLEQVADETNISKHFLQGIESDDFSDF
                     PGEVYSLGFIRNYAEFLGLDASAMVEIYRSNESTRQAEVEKSESTELPLPVEDSKKEE
                     TLPQKSFISSEVEKSAKETKQTFHAKRKPKEKSIKREEPETIKAEPKASIQVDSTLEK
                     AALIDFQPKKKIGTANIRVGRVLPFVFVAIVLIIAAVSIIPRLRISPGAARVPTEYRA
                     EGLPFEQRLYPQDKVYIPLSGDVISITLESIKDKVSFNTPFGSLAVGLNEKTVVDPSA
                     DRERLVIAVSDYEADKSQNGAMVHFDVEEALSDAENSLDIIVPSTPGKAQLSQDSTQG
                     QAGGANRTQTSSTVLFRSTGGPHPFYVNVSFIAPVFFRYEADRKEWVEKYYRKGESIT
                     VNASNTITFWTANAQAVKVNVFQSAGKSVELVMGGPGEIAVQRLSWSNSQSGWAMIAT
                     QVD"
     gene            complement(34316..35002)
                     /locus_tag="PLQ30_00995"
     CDS             complement(34316..35002)
                     /locus_tag="PLQ30_00995"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPW01051.1"
                     /translation="MKRKHITIIASIFACFLGASTLSAQVLLTADQFFSQLAEKYAMV
                     TDYQADVHITAGHQPMAGTLLFKSPSLLRIDFSQPADQVIVFDGKTLLVYLPQYRAVL
                     RQDSTDQGISVGAATLASKEGLSLLRRNYTIGWEQSPNPEPLDPGSDELVYRLMLSRK
                     SVSEGYKNIRISVSADTMLIRRLEGWTVSNDKITFDFVNIQLNRGISDNKFIYNAPAN
                     ANVYSNFLFQ"
     gene            complement(34999..36660)
                     /locus_tag="PLQ30_01000"
     CDS             complement(34999..36660)
                     /locus_tag="PLQ30_01000"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HPW01052.1"
                     /translation="MFETSPLHAGRYSYVPKMPETFLDSIQSIKTIPTETIEPERDRE
                     VLKKFFPNSYGKPAIKFVKGEGALEDKAITVGVILSGGPAPGGHNVIAGLFDALIKAN
                     PQSRLLGFKSGPSGLIKGQYVELSSELIDQYRNTGGFDMIGTGRTKIETSEQFEQSLE
                     NAIKLGLDALVIIGGDDSNTNAALLSEYFFQKGAPITVVGVPKTIDGDLKNEWIETSF
                     GFDTATRVYSELIGNICKDASSGRKYWHFVKLMGRSASHITLECAFQTRPNVALISEE
                     VDAKKMSLSQVVEIIANTIAARSAKGENFGVVVVPEGLIEFIPEIKTLIAQINDIIAT
                     SEDEYESLPKGEAQRSYILSHLPSECISLFSSLPESIQTQLCWDRDPHGNVQVSRIET
                     EKLLAEMTSRKLQEMTAVGAYKAKFDYQTHFFGYEGRCAFPTNFDADYCYSLGYDACL
                     LILHGLTGYIAAIRNLAAAPKDWLPGGIPIVSLMNIEHRHAEEKPVIKKALVELEGKP
                     FKAFSAMRDKWALSSDYRIPGSIQYFGPPSITDTPSITLQLEQRL"
     gene            36791..37813
                     /locus_tag="PLQ30_01005"
     CDS             36791..37813
                     /locus_tag="PLQ30_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPW01053.1"
                     /translation="MVRRILKASIFFLLFGLLLTISGAEQEYHVIQAGETLYSIAKSY
                     SVPYETLASINSITDPSKIRPGVVLLIPRVHIVAKGETYYGLSKQYGISIQELKSANN
                     LSDIYVLKVGDVLIIPEKGVISVQTVQDSPPLDSVVDSVAMSQPVASASTQQNSSSPA
                     NTAIVPSNSVSTTSSTSQAVLPPNSPTTSSSAASQSTNPPAQTSAPTKADSPKTSVSG
                     KRIPDAIVNWPVDGKAQYMSGKLEGVMFQTARGASVKAVASGTVVSAGPSRGYGEVVF
                     VQSKAGFVYVYAGNESILVKTGEIIEPGKAIAKVGVDANDGNPIAYFFVFRNGQPVDP
                     SLAPRD"
     gene            37924..38910
                     /locus_tag="PLQ30_01010"
     CDS             37924..38910
                     /locus_tag="PLQ30_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPW01054.1"
                     /translation="MGDYLDCDKASISKLPSRKGSQEKAEQESDPLSLYLRQIARFPL
                     LDAKQEQEIGVKLEELGLLLKNYDKEITKDPQNIPLLEMRRNTLEKIRASKEVLITSN
                     LRLVVSIAKGYRMRGVSLLDLIDEGNIGLIEAVNRFDYKKGYRFSTYASWWIRQAIIK
                     CLVDQSRMIRLPIHMLNTIRQCYASAKGLVQELGREPNSIEIAERSGIALSKVESALL
                     LALGTSSLDLCTKENQNTSPVNNLKDEGSSDPYSDAFLVTLRELLSYVMDSLSEREQA
                     VLELRYGLNGEAPKTLEATGLALGITRERVRQIQERALDKMRNRRELSDSIQ"
     gene            complement(39052..41853)
                     /locus_tag="PLQ30_01015"
     CDS             complement(39052..41853)
                     /locus_tag="PLQ30_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPW01055.1"
                     /translation="MQRRSFKVGRFFSAIIAVLFLLGGCATSPAKNKEVTLNDLVIQG
                     DFEGIRKFYSNQEELNKPSNKEPYIGLYPLHIAVTQGNAQIAEILIVLGAKPDNQDLS
                     GKTALRYAVDRKQTAMVKMLIDRGADPFITDVGGTSPALVALHSDISLLDAMFNSRNI
                     NNTGPDGRTILHHASDALMPKEVDLLLSKGALVQIKDKADRTALDLALLYPEKLEAAQ
                     IAEKLILKGANPSFPEFSWFADTVRRPDYGAARFANGNTPLHEAISRYQHGFALFLLL
                     NNVDPNLKNLNGDASLHLAVKMGYVEGARLLLSHNANPDILDANQSTPLHLTVPATQR
                     LEMVKLLLAFKANPALMDIQGNTPLHKAVAQLYAPEIVETLIQAGAPVNAQNSKGDTP
                     LIICARTEHYEYARPLIEANAEIFKMNQAGENALKIALSKGYTAVDNIVLPSNVNQSD
                     DNSNSVLMTAVSLKASPDVLKLILSKGADPNAKNKAQDTALHISVRQNYAQQGIVLLD
                     ANADIFQYNAVQENPLFLALTAKPAPLEWFFRPNVITATDANGDSVVHHAARKNLPEG
                     ISYLVKKGANIEALNNAGEIPLHTAAKYDATDAIRYLVSAGSSLNAANKNGDIPLQSA
                     VLNGAFKATQMLLAAGSPVNNQNYSGETALHQASKNNSAMIIQLLTAQGAQTEIQDSR
                     GFTPLITAAANDRYDAASELIKAKAVIDTRDYLGSTPLYHAVSGEYAALIRLFLVNDA
                     DIHALNAQELSPFKLSLSKNPQITPEFYTDKLINKPDSSGDCVLHILVKSGQGQEALT
                     QALKHGANLNARNSKGDTPLMLALKKGDLNAAVVLIQAGSSLFVSNADNKIPLSVIFG
                     MDSGTRMKLFSAAGTNSADFLGESILHYAVRENNADVVSELLSLGADRNAHNRKGETP
                     KDIAQAKGYQEILAILERK"
     gene            42013..42990
                     /locus_tag="PLQ30_01020"
     CDS             42013..42990
                     /locus_tag="PLQ30_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HPW01056.1"
                     /translation="MKIGSFMPIQSIWRTIIEQLREEFPDHTWLEGLTSDSSEIGSLD
                     LIIAGRIPADVFKAATNLKALFQPFTGINHLPSQLMLERNIQVYNVHSNAFDVAEKAL
                     ALTLAFYGRVIEYHNDLKNEIWHGFWVNRGAEDNWDTLYGRNCSILGTGAIGQELAGL
                     LKAFHCTVYGWRRTKDAKKPAHFDSIVPTLQEAIDFAEIIFIALPATKETENLISKEM
                     LMNMKGKFLVNVGRGSIVNEEGLYEALKNGVLKGAAIDTWYTYPATGVVGAPSRYPIH
                     KLSNVVLSPHVGGSTNRASAKSIEDTVANIRSYLRTGRGIWQADLSKMY"
     gene            complement(43033..>44532)
                     /locus_tag="PLQ30_01025"
     CDS             complement(43033..>44532)
                     /locus_tag="PLQ30_01025"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW01057.1"
                     /translation="EVVKPMPITSKSPFNSLICGEGEEAFADILQDILEHRPISSFYS
                     SDKLIDLNKLPNPYLNGALLFDPNFQVHIETMRGCNSKCSYCYYGKNYKTVRYFPHEQ
                     ILEVIRLASEAGIPEIYIMDPNFQIRPDFAGKLRDIAFVNHAHTSIHTELRLEDIDEE
                     IAGLLKEAGIVSVEAGLQSTNSKALEAIHRSFDRKAFERGAELLQRQKIIIKTGLILG
                     LPYDGYEQVIETFDFLGMQGLGQEAELYPLSLLPGTEIRERADEYGMSAMDPPPYLVT
                     STHWISYDDIVDAISAFEESFDVEWAMPPAPHFQLFKEGFVSFIDLRKPENIDWMRLN
                     PEKLSNSITLLVDADDPEILSRVVRAARDLRKDNPYTLYQIVLTSETRIPSEKLVERI
                     RDAFMNSAHYYELMNSFSPDPQTSYQTRIFFATKNFTLAYRALEEAQDMETMVILSGK
                     GGYNSDRLAELLPYVIFDKLSLPFDRLYELISIYADFPHMLIEAPEGLF"
BASE COUNT        11907 a         9748 c        10420 g        12357 t
ORIGIN      
        1 ccttctggca caaagatatc tactcctccc attagggcaa atacatctat cgtagaaacc
       61 ccagggggaa tctgagcttg tgagtagtcc aggtcgacac cgcccattat agcgacgact
      121 ttcatcctct tgggtggata ccataagccc tttctagtcg caccagacaa aaaagctagg
      181 gtgacatcat aatccttgat ttcaccgtgg ttgagcaata ttccatcaag agatgtaaat
      241 tgcttattgg tctcggctga agccggaaga tcttcgatta gagataaaag ttctcttttc
      301 gacgcggtgt ttacagcaat ctccattcgc ttctcgtata catcgatatc gagcagattt
      361 tgcgcaaaac cgtgctctaa gcgctcgatg acattatttc tctcgatttt tgtgggacca
      421 tgatcatact tttctagctc tggcattatg acctctccag aaactcaacg atattatgcg
      481 atattatgcc aataatcttg ctaaaatgta agcatgagaa gcataagcta atctaatata
      541 caggatttta acccatggaa tcattgcctc tcgccaaagc ccgatcaaaa cattattatc
      601 atctcatact atggcagaaa agaagattcg ttgcagtacg catatgagca atctttgcca
      661 ggaaaaggat ggcaatctac atgacttcga attgcatcca aggggcaggg gaggggcggc
      721 gatcatctat ccggttattt cgcgacgagc tggagggctt tctttaggca tcaacctttt
      781 ccctgagcgc aagatttgca gctacgattg cccctattgt gaggttatgc cattttccaa
      841 tcctggggct tcgctaaaag ccggcgatgt gagccgcgct atcgagtgct tcttcgatga
      901 agaatggcct gagctctcaa agactctgga gctcaaagat atttcgattt ctggaaatgg
      961 cgaacccaca ctctcgccct tcttggaaga agctttgtat gctgctcgcc aagcaattga
     1021 tacaaaggcc ctcgtaaagg attgcgcaac accatcaggc cagcactcga ttccatctat
     1081 cgttcttatc accaattcca ctggcttcct tcgtgaagat attaccgagc tccttgccgg
     1141 tttttataaa agatacccct tcgaaatttg ggcaaagctc gatggtggaa aagaagagct
     1201 tcatgctctc ctttcgcgct cgcgatttcc gtttgctgaa attgtccgtg ggattgtgga
     1261 cttttcatgc agtgtgcctg tcactctgca gactatggtg atatatgaca atcgaacagg
     1321 caatatttac ttcgattgcc agtcctatgc tgtcacgatt agagaaattc tcaatggtgg
     1381 cggacagcta agagcaattc agatatatac cacagcaagg gttccagtcg aatcatgggt
     1441 tgcgcccgtg tcggatgaga gactagtgat ttttgcacgg caattatctt caatattaaa
     1501 tagaccccaa agaaagaagg ctaccggcaa agaagaggaa gcattacaaa atcaggtgta
     1561 ttcgattccc attttatgtt atggatcgcg gggcaaactg gattggaacc atgccaaccg
     1621 ctgaccttca cacccactcc actgcctcag atggtgagct tacgcctgcc gcgctcgtcg
     1681 aaaaagcccg cgcgcttggc ctttccaccc tcgcccttac cgaccacgat aacactctag
     1741 gagtagcaga agcgcgcgcg gctgccaggc ttgtgaacct tgaattcatc cctggcatcg
     1801 agatcgagat agatttcgag ccaggcgagt ttcatcttct cggttatggg atcgacgagg
     1861 cagcgccacc tctcgtagag gcggtggaaa gactttccca cgctagactg gagcgcaact
     1921 tagccattct tgatcgcatt gcgaaagcca atcttccact ggaccttgat agtattgaag
     1981 ccttttcgaa aacctcttat ctgggtaggc cacacctcgc agatcttttt gtggaagcgc
     2041 attgcgctcg tacgcggcag gaagcctttg atcgatatct tggcaaaggg aaaccattct
     2101 atataaagaa agcatgtcta gcgctcgaag aagcgctatc cgttattgat gctgcgggtg
     2161 gggtttccgt tcttgctcat ccctattcac ttttcgtatc aaaagatgtt cttggcacga
     2221 ttttttctca attgaaagcc aaaggcgtta ggggaataga agcttaccat ccaaccgcca
     2281 aatatggtca atgtgttatt ctggaacgaa tggcacacga gcgaggaatg tttgtcacag
     2341 ctggctcgga ttttcatggc aaaatgaggc ctgaatgtgg gctgggtcgg acttcgggaa
     2401 ataagcctat agatattcgc tttcgagatg agcttgtagc tgctcttgaa agccgtattt
     2461 gatattcttc tttgaatatc gctgctcatc ttcgccaaat ctcaaggcgc tctcgatatt
     2521 attgtctatt gataacaata tgccttgata acgatagcaa ttaagaataa atgcctttag
     2581 aaacagcttc aagcattatt tctcgctttt attgtccgaa agttcacttt cctcgttcaa
     2641 tgccctgtag agatcaccag cggcaacaaa tagtatcaga ataacgggac ccaggatcag
     2701 cccgttaaat ccaaagactg ccaggcctcc ggttatggag aaaaaaagca agagtggatg
     2761 tatctttatc tgttcaccca atacaattgg tcgaataaaa ttatcgagcg tcgcaacgat
     2821 tacgcttgcg caagcgacaa agacaattgc ctttataatt tgaccgttaa ccgcaagata
     2881 aatccccatt ggagcccata ctacggtagt gccgagaagc gggacaaagg atgaaatggc
     2941 tgtcaacacc gccagcacaa gattattctt gaaaccgaag ataatgctca ggatgaacat
     3001 cataaaacct tgaaaaagcg aaacgagaaa atacccgata acaagttgac ggcctgtttc
     3061 tcggagcttg ctcataaaaa gcctggtgta ctgaggagct ataggcatca tggaaacaaa
     3121 ggtgtctccc aaagaactag catccatcaa gagataatag agagtaaaaa ccatgaaaca
     3181 gatagtaacc acaaacttgg agatattcat tatgagggaa gtagatattt ttataagttg
     3241 gtttactccg gatgctagga atgactgtag ctccctcagc ggatcgaaag tactaacatc
     3301 atatagatca ccaagcagag actgaattgc tgagcctatt tgattctgag gatcgattct
     3361 caacttatcg atattctcct ctacaaagtg gatgctcaat ttgagcaaat ccgcaagctg
     3421 ttgtattgtc ttgaatgcga gaaatgcaag aggaataaga ataataatga cgccgaaaat
     3481 ggataaaaac aaagcgctaa gtcgagagcg catggttttt cggcctttta tactattagg
     3541 acctctgagt cttttatgaa ttggctcgag aaagacatac aataagccac tccatagtat
     3601 tacggttaga aaaggataaa agagccttcc aaccagaagc aataatgcga ggaataggat
     3661 caaaaaggag atctttcgcg gatttgtggt cttcattgtc cgtaatgata attgttattg
     3721 aggtttatga atagatatat cttatctgga gttggccgca atcgcagttg gagccgtagt
     3781 tggaatcgta ttggaaccat agttggttgc atcaaagata tcgaggcatc taaagataag
     3841 cctagacata atagcttgta atcgtacaca attgtctctg tgagcacact tttcatgatt
     3901 gcaacaccta ttggtaatct cgaagacata agcctgagag cgctagaaac gcttaaatca
     3961 gtagatatta tcgcatgtga ggacaccagg cacacattaa agctgctttc gcgatatgaa
     4021 attcgtaagc cgctaatctc atgtcattcg tacaatgaac aacaagcagc caaaagggtt
     4081 atagcccttt tgagcgaggg caaagacgtg gcctattgtt cagatgctgg aacacctggg
     4141 ttttctgatc ctggagcgct gctggtgcga atggcgcgat cagcaggata caagatcgta
     4201 cccattcccg gaccaagcgc ttttgcctgc cttatcagcg catctgggct atctgggaag
     4261 acttttttat ttgatggttt tctttcgcca aaaccaggca agaggcggac aaggctaaag
     4321 gaacttcttg accgcggaga atcctttgtt ctctatgaat cgccttttcg aatcgggaaa
     4381 ctgcttgctg atatagcctc aatagatccc gaaagaaggc tatgcatagg cagggagcta
     4441 acaaaaatac atgaggagat aatcgaaggc aaggccgaag agctggttca gcgatttcct
     4501 gcggaatcgg ccaaaggaga attttgtata attgtgcatg gcataaagcc tgccaattct
     4561 gtgaatgaca aagaagatga atcatgatgg atatctctca ctttaggaat ctccatggtc
     4621 gtgctagatt gcgaaaagcc gtgcttttgc tcgagaggct agaaagagaa tggcgtaagg
     4681 ccgatcctct tatcattcct tacgaagatc ttgtttttgt caagcaaata gctaagattc
     4741 ttgctgattc tgaagagacg acattggaat gcgtgaaggc tgccagcgat tttcttgggt
     4801 ctctgtctgg gctcgacgat attcttgatt attacggttt tttgcaaact gaaaacaaat
     4861 ttcggcatag acttatgatt ctatccggcc agggcgtcgc agattgggat ttcatagccg
     4921 tgcccaggtc ttcggctggt catgccacaa agagagcggg catgcgagta tatttggaag
     4981 atttgcgctc gccttttaac gtggggagca tctttcgcac cgcagaagct cttggtttcg
     5041 aagagataat tatttcgccg gaatgtgcgg atcctatgca tcccagagca atccgaagcg
     5101 ccatgggaac catagagcgc atgaaatggc gaagagccga gctgtctgag cttgattgta
     5161 ttcagggagt ttttgctctt gagcttggcg gtattgctat cgacaagttc tcttttccat
     5221 ttccagggtt gatggtcgtt ggctctgaag agcttggagt ctccaataaa gcgctgcaat
     5281 attgcaggtt gggatgcatc gagattcctc tggttggaac aaaggcttcg cttaatgtag
     5341 cgacagcatt tggtattgca gcgtattcat ggtattattc tggaaatatg caggcatgat
     5401 gtgtttatat tttcttgtgc tattttgcca cctgcattaa aactatatta gcgaaaggaa
     5461 tgctgaatgt ctttaaatcc ttttatgaag aagcttggtc tttcatcttc cgacagggcg
     5521 gtcatagtcc acgtagatga tgtagggatg tgtctttcat cgatagaggc tttttttgag
     5581 ctatacgatt ttggtactgt aacatctgga gcactgatgg ctccttgccc ttggtttccg
     5641 gaggtggcgc gccgctgcgc ggcgcgccct gatgtcgatg taggcgttca tgcaactctg
     5701 acgagcgagt ggaaaggcta ccgctgggga ccagtatcga ctcgcgatcc agattctgga
     5761 cttctggatg ccgaagggta ttttcccatg accagtgagg aggttcaatc tggagcgaaa
     5821 cccgaagccg caatgaaaga aatcgcggct caggttgcaa tggtatatgc tgcaggcatc
     5881 gagcctaccc acatcgacac tcatatgttt tcggtagcgc atgaaaagct ttttgacgcc
     5941 tatatatccc aagcccttga acataggata ccggcgatgc ttccaaagcc agatatcgtg
     6001 ggtttcgagt ccattgttcc ggattgcgaa acccggaagt accttgctgg atatcttaaa
     6061 gagcttgggg aaaaaggcat gcctctgatg gacggcattc tggcgatgcc cttagatgaa
     6121 cctcgcgatc agaccgagac cgtcaaacgc cttcttggtt ccattaccga aggtattacg
     6181 catttcgtgt tgcatgcctc aaccgacagc catgagctga gagccattac accagactgg
     6241 gaaagcagag tctggaatta cagaaccatg cacagcgaca ctatgaaaaa aatcatagag
     6301 agcgagggga tagtcctaat tggctatcgc aacctgcgcg atgcaatgag gaaatgatct
     6361 ggacatctcc gaagctgggt gaaggattta tgcatagaaa atcaagctgg ggtatacgat
     6421 aagccttggt tgacgccttt tataaatagt cgtaaactgt cttcataaca tacacctctt
     6481 gaaaggagga tccatggcaa aggttgaact caagaatatt ggaaaggtgt acgaaggcaa
     6541 tgtgagagcg gtctcagatg ccaacatcaa catccatgac aaggagttcg tggttttcgt
     6601 aggcccttcg ggctgcggta agacaacaac gctccgaatg atcgcagggc tcgaggatat
     6661 cactgagggc gaaatatata tcgatggtag gctcgtcaat gatgttccgc ccaaggatag
     6721 ggatatagcg atggtttttc agaactatgc gctctatcca cacatgaccg tctacgacaa
     6781 tatggctttc ggtctcaaga ttcgcaaatt acctaaggaa gagatcgata atcgtgtcaa
     6841 ggaagcagcg cgaatcctcg atatcgaaaa gttgctagac cgcaaaccca agcagctttc
     6901 gggtggacag cgtcaacgag tagcagtagg ccgagccatc gtgcgcaagc ccaaggtttt
     6961 tctttttgat gagcctctct ctaacctcga tgcaaaactg cgtgtccaga tgcgtgccga
     7021 gctaatcgaa ctccacgacc gtctccaatc tacaatgatc tatgtaacgc acgaccaggt
     7081 agaggctatg acaatgggcg acaagatcgt cgtcatgaag gatggactta ttcagcagat
     7141 cggttctcct ttgtatctct acaataatcc gatcaacaag tttgttgcag gattcatagg
     7201 ctctccaccc atgaacttca tgagcgtcaa tgtcatagaa gaaggcgggc aggttttgat
     7261 cgacgaagga tcattcaagc tcgctcccga tgcaaagcat gccgagcttc taaaggctta
     7321 tgttggtaag agtgtctact ttggtatccg accagaagac cttcccgtgg ccgatgctgt
     7381 acatcgtggc cagaagttca atgcaaaggt tacggtggtc gagccgctcg gttcggagat
     7441 tcatcttcag gcaaccacgc caactcaagg catggtgtct cgtattccac ctcatcacct
     7501 ttacaaacac ggcgatacaa tcatttttgc tccggtaatg gcaaaagcta tatacttcga
     7561 taaggaaaca gagaagagca ttcttccagt aaaatggaac gaacaagagg cctaaggcta
     7621 agaacgatag ccaaagctct gctattataa accgacttta ttgatttctt tttgtggtaa
     7681 tcaattgaaa atgaaaagcc gcctctattt aaggcggctt ttttagggcc aatcattcgt
     7741 tctctttaga ttctttttta gcgcgtttct cagcacgctt ttctttgagt gttttttggg
     7801 gttttttctt atcggccgtt ttaggtttat ccatcgattt cataccgcac ctcttattct
     7861 tattatcgaa aaaactgcag agcttttcaa tcacttctgc gttgcgttct gaatttccga
     7921 aggttcacct tttgtcacct tacccattat tgcacgagcg caaatcatac agattgtaca
     7981 gaagaggaat attccctggt atccgaaaat atcgattatg acgcccgtca atacagggcc
     8041 tagaatagcc gcagcttgac ttgcggtata ataaaggcct gtgtagattc ctattgtgcc
     8101 ccattctgcc atctgccata gcatagggaa agagtttgtt attattgata cccagaaaat
     8161 cccgaaaaga aacatgatcg cccagaatga gcccaacctt aaggaatttg atagaccagc
     8221 ggtaatccat ttgtgaaaaa ataggagaag ggtgagaatc gatatcacca aaaggcatgt
     8281 acgaattgtc ttttttctgc caaagcggtg tgcgacatag cccgaaggga tagcgaatat
     8341 ggcataagct atgcccacca ttccggctgc cagagccgcc ataccgcttg aagttctgag
     8401 cacatccacc gaatacttgc cgataaatgg caagacaccc tgatatccta aaaaccatag
     8461 aaatattgcg attaaaatat atagcgcgct cttgtctttc tgcgcggcaa catttcggaa
     8521 gctctcgaga ataggcactt tttgttcttg tgtttccttt gcatccggct gcttctcacg
     8581 gatgaatatg aaaagaagga tcacggctaa aatgacgaac aggccgccaa gtgggaaggg
     8641 gagcttatcc ttggtaggtc caaggatagg cagcgttata tcgagatcca ttaatctcgc
     8701 aaggccgatt gttcccacaa tgcttgctat gccgcccatt gtgttaatga caccgttcgc
     8761 ttcggagcgg taatcggccg gtatcgtatc aggcatgagc gcaaccactg gtccgcgcac
     8821 tgactgcttt gttacattta gaaggatcaa tgttacaagc aaagccacga gagatacttc
     8881 cgtagcatag ggtatagcgc caaaaaggaa agcggtaagg ggcaaaagaa ccagaatgta
     8941 gggcatccga cggccaagct tggtataggt attatcggac caggcgctga caagcggtat
     9001 caaaaaaatc gccagtacat tgtcgagcgt catgaaaaag ccgacaagac tcattttgct
     9061 taggtatcta ctgaggaata tcggcacgta ggtatcgtat aggggatcca taagacccat
     9121 tgtgaagaag ccgaatccga tcaaaaaagt tatgcccaga taaggcttaa accctttttt
     9181 ctcgctcatc ataaccctcc ttgctagtat taatagagca tttaattcat aagaacctta
     9241 catacaccaa atggccggat attaatgcgc tgtacgctgc cttttccaaa gaccgcctcc
     9301 atgcgctcaa caaattcagg gaatcttgac tgtggaacat agacctggat tgtacctgca
     9361 aaaccaccgc catgcacgcg ccatgcgcct tccggactca atagatcttc gctaagcata
     9421 agggctatag aaaggctctg ctctcgcggg ttgccagcat ggatattctg aaggtaatgc
     9481 cagctcgatc ttccggattc attaacaatt gatagatagg cagatttgtc atttcttttc
     9541 aaggcttcca ccattcgtgc tggacgatag ttttcataaa cgaaatgcca ggcgcgcaat
     9601 aaagctctat ctccgcattt tatgcgtatg tccgaaagcc tattgagaag gtcttgcttt
     9661 tctgttcctc gcagagcagt tcttcccagg atagctgcaa cacatttcat ttccttggga
     9721 atgtctgcat attctcttgt aagatcctcg tgattggaac ctgtattgac gatggccaga
     9781 ataaggccat tagattcaaa gtcgtatatc agtctatcga tctttggcct tcgagaatcg
     9841 gcgaagtcta tgaatacgat gccaccttct gcgcatgcaa tttggtccat aaggccacaa
     9901 ggttttccaa agtattcatt ttctgcccat tgtcccgcta tagcccaatc ggttggtgtc
     9961 aaatcatact tgcccatgtc gtcgaatatc gatgcaatca aaagctcaaa agccgcagag
    10021 ctcgacaagc ccgagcctgc cggcactcta gagtccattg caatatcgaa accagggcca
    10081 atctgcaggc cttggtgagt cagccaagct gccaggccgc gtattatcga agcaggtgaa
    10141 cctttttcct caattctggg ggcggtatcc ctcaagtcga ctcttatggg agagtagcct
    10201 tcagataaaa gggtaaccat tgaatcgctg cgcgggcggg caagagcaag catgtcgagg
    10261 tctactgagg ctgcaagtac acagccattg ttgtggtcag tatggttgcc acctaattct
    10321 gtgcggccag gcgaagaata ataatgcaaa gcccgagaat ggctatcttc gccgagaggt
    10381 aatctagata caagcgaatc gtatctggct ctgcgtcctt ccttttcgag atcttggaat
    10441 gaggcaagag gaatgctagg aatcttctgt tggatcatgg cttgctcttg tctattgtcc
    10501 tagaaaatcg agataaaggg aagagtcgcc ttccttgtat tcaagataaa tatcgcaaaa
    10561 gagcctctcg gaacgaaaaa gtctttttgc attctcttgg aattctgcct tgctcccttc
    10621 caagcctcca tcaaaagaga aatgctctaa atagataaag ctacgagttt gcattgctat
    10681 atataaacat atatttatat ctatgtttat tgtgtcttat ataatgcaca ttgcggtgtg
    10741 cggagaacgg tgctaaaatc aaaaatggat ttccaaatcg ataattcaag ctcgaggggt
    10801 taatatgaat cagctggcgg aagaattgaa tgctgtcctt gcgggaactg tggctgagag
    10861 actgctttcc gaaatgggta ggcgtatgta ttttcctaga ggaatcatca cccagagtgc
    10921 ggaagcagga gaaaaagctt ataggttcaa tgctacgatc ggcatggctt atgagcatgg
    10981 gcagccgatg atgctcgatg cattgcgcgc cgaattgccc ggtttgactc caacagaagc
    11041 agtcgcctat gcatccacag gtgggattgc agagctgcgt aaggtatgga aagaatcgct
    11101 ctatagaaag aatccatcat tagatgctaa aaagatatct cttcctgcgg tcgtccctgg
    11161 gcttacagcg gcggtatcct atatttgtga tatgtttgtc gagcctggcg atacagtaat
    11221 tctgcctgac ctacactggc caaactatag gctcatcgta gaagaaagaa aaagcgcagc
    11281 gggcataact tatccactat ttgctgggaa gggctataat attgaaggct tgcttgaggg
    11341 tgttcgacaa gcagggcagc gtcggggcaa ggcgataatc atccttaatt ttcctaataa
    11401 tcctactggc tattccttga cgcttaagga ggccgatagc attactgccg ggcttctaga
    11461 aatcgctaag cagggaacag atatccttgt aatagccgac gatgcgtatt ttgggctgca
    11521 atatgaagat ggtcttctct gtgaatccat gtttgcaagg ttagcggaca tccaccccaa
    11581 catactggct ataaaggctg atggacctac taaggaagac tatgtatggg gattccgcgt
    11641 tggttttgtg accttcggtg ctgctggttt tggcgatgag cattatgaag ctcttgctaa
    11701 gaagctattg ggagtaatac gttcaagtgt ctccagttcg agcggtcttt cgcagaatct
    11761 ccttctaaaa gctcttaagt accaagggta tgaggcgcaa aaagcgaggt atagggatat
    11821 cctgaaaggc cgttacaagg caatgaagaa taaattggcc gagatgacgc tgccggatgg
    11881 tctcattccg atgccattca attccggtta ttttatgagt ttcagatgcg atggcttttc
    11941 tgcagaagct cttaggaaga agctcttaaa cgagaagggg atcgggaccg tgtctcttat
    12001 ggaccgctat cttcgtgtag cattctccag tgtagaagag agcgatattt cagacctctg
    12061 cgatgcaata gcgcaagcag tggttgagct ttgtcagcaa ggctgaggaa gaattgtacc
    12121 caagagtgcg cagaactata tctctttagc agtcatgctt gactggtctt gagctgattg
    12181 caggacatag gatttaaggg gcgggaagcc attgaaatag atggacgaat agctcctggt
    12241 atatgcaccg gtggtcagaa tatatacctt atctcccacc ttagctgaag atggcatgaa
    12301 atatggagtc cgctcataga gtatatccat agagtcacat gtgggtcccg caagaattat
    12361 ctcttcggct gagccttgac cttcgaaata aatcggatac ttgatcgatt cgcccagtgt
    12421 ctcgatgagt cccccgaatt ttcctatatc gagaaacacc catggatatc tatcgtgaac
    12481 ggatttctta gctatgttga caatttctgt gacaattact cctgcatctc cagccataga
    12541 acgccccggt tcgattatga tcttttctgg ccataccaaa ccgaaactgt tatctagaaa
    12601 acgctttatg tcctctgcat attgctggag aggatcggtt ggatccagat aattggccgg
    12661 aaatcctcct cccatattga tcattttgag atcgatcttg aggtcgtggc gagcccaatt
    12721 aaatagctgg gcggctatag tgagtgcgct tgaccactgg cctacatcgc gttgctggct
    12781 gccagggtgg aaagatatgc catatggttc aaggccaaag cgaattgcgg tcttgattag
    12841 ctgacgggca agatctgggt gggaaccgaa tttttttgat aaaggccagt ctgctcctag
    12901 gccttccgtg agaagcctaa agaacacctt actgccaggt gccgcgcgag agagtttatc
    12961 gacatcgtta atcgagtctg ttacaaaaag ccttacgcct ttttcataga agtacgcgat
    13021 gtctttttct tttttgatgg tgtttccaaa gctcattcgg tctggagata tgcccaggct
    13081 cagaagttga tccagttcat atcgagaagc aacatcgaag ttagagccta gatctctaag
    13141 cagagaaata acagcatcat ctggattggc tttgacagca tagtaaatag ttgcccatgg
    13201 aagatatgct cttagatctt cgaagttctt ttttatgatg tctaagtcta tgatcaagaa
    13261 tggggtctct ttgtccctag agaattcctt gattctttca aatttctgac ggttaataaa
    13321 gttttcgagg ggaaaggaat aggcgttttt cactgatccg acctcttcga tttaagatag
    13381 ctctttagag ctccaagcgc atacattatg catctacttg tccaaaggtc aagcatggag
    13441 tatagaaata gtatgattac attttttata aagaaagcct ttttcgatgg ctgggataat
    13501 ctatatctat tggcagcctt gaatgggatt tttcttggcc ttcttctttt attccttgta
    13561 gtcccaattg cgctggcttt tcctatttgg ctttctataa ttattgctgg gattggaatt
    13621 tttctacttt ctatatggga tgtagtggtg accgatacaa tgtatggcat agccgatgga
    13681 aaaagcgtcc attttgcgga cataaaggtt gccattaaag gatcgattaa gcttggcttg
    13741 ttgcttggcg tagtcaatat tttaatagca gtagccataa tggtagctat ccccttctat
    13801 cttggccaga aagcagtttt aggtatcttt ataggaggcc ttctattctg gatgatggtt
    13861 ttggccatgt tgattttgca gtatgtgcca gcagtctttg cacgcgaaag aggtactccc
    13921 cgccaagctt tcaagacagc aatttatctt tttatcgata atccaggctt ttcaatattc
    13981 ttgtttttct ggagaatttt aacattcgct atatcgatat ttgtggtatt gctggcccct
    14041 ggacctgctg gaagctcact tgcatccgca gtagcggtga ggcttaggat gaaaaaatat
    14101 agctatctaa atgaaaatcc cggagcaaac agacgccata taccatggga tgatcttcta
    14161 caggaagaga aagagagagt cggcaagaga acgttgaaag ggatgatttt cccctggaag
    14221 gattgatcat gatcgataaa tcgaggagag aaccaggcat gaaaccgcca tcgcgagaca
    14281 agagccgagg aaatcaaaag aaaggacgag caaatcaaga taacagacaa cttttgcccg
    14341 aagccttcga agagcgcaag aagcaagctc tttcgagcat cttgtatatc cgcttcgagg
    14401 gtgctgatgc acaatcgcgc ttgccatcag gaatcgatgc cagtattcca tatcctgtcc
    14461 agctacaggc tcccgcggtc aaacttgatc cttcatctat tactatcgaa agccttttgg
    14521 caggtatgtt aagagtgctt gcttgggact cagagaacga gaatgccaat aaatatcgaa
    14581 aatatataaa gatgtcccgt ccagagcttt tcgaggagtt gatagtggcg ggaattcaga
    14641 aagccgaaga aaaagattgg atcgttgctg aggaggtttt cttcgcggca agcggccttg
    14701 agccggagcg gcctgagcct tatatcaatc ttgcgctcat gcatgaagaa cacgctaagc
    14761 ggctttttga ttctggaaat gaagatgaag ccgaaaaaga agacgatctg gcatataaat
    14821 actaccagta tcttctcgga ttcaaggatg tgtttgctcc cgcatattat catgcggctt
    14881 tctttttctt gagaaagcac aactatgaca aggctgtttc cctattgaca agttatatcg
    14941 ggatgaatga tgacgaacaa cgtactcgca gagccaagtt aattcttgaa aaacttggcg
    15001 gcatgggcta tctcgatacg cttttcaaag aagcatacga tttcattcaa atggggcaag
    15061 aggaaaaggg gcttgaaaaa gcaatgcagt ttgtagaaaa gtaccccgat gtctggaatg
    15121 gttggtttct ggtaggatgg gcaaatcgca ggttggggcg atggtcggaa ggtgcccaag
    15181 catttcatac tgctctcgaa aaaggagccg atggggctga tgtctacaat gagctatcta
    15241 tctgtgagat agaggcaggt gatctcgagg ctgctaggtc tcaccttgaa agagccctga
    15301 gaatagagcc agaaaatgag aaaatcattg taaatctagg tgctcttgcc tttcgccaag
    15361 ggcatataga tgaagcagaa ggcttcttta ggacttcaat cgaattggat cctgatgata
    15421 gaattgtcaa ggaatggatc aagaagatcc aagctttgag ggaaagaaaa gggactggag
    15481 cataagaaca gcgatatcga gatgaccata agaagcgagt ttcggagttc ggaaccccct
    15541 gctgccgaag aacttgaggc agcgatagat gctgggattg ctgcaaatct tcatgtttct
    15601 gcagaattga gaccatccga tccagccaat tctctgccgg gaggcttgat tgttcttccg
    15661 cctgataagc cctgcatact tgtccctgat ctgcatgcca ggccgagctt tcttcatgcc
    15721 ctgctgctaa ccgagtttcc aggccttagc gaaccattgc tttctgtgct ttcgaagaat
    15781 aatgcaacac ttctctttct aggagatatt ccgcatgcag agggcgaaga ggctgccaga
    15841 cgctggatta gttcttatga taaggctttg attgctcgtg atgtaagagc acttctttcg
    15901 cccgaaatgg atgaagagat gggcctctct ctgacttcct tgctgaaagt gatcgaacta
    15961 cagtgtggtt ttccaaattc ggtattttgc ttaaagggga atcacgataa tatgactaat
    16021 gctggagatc atggtgattt tccattttat aaatacgcag atgagggcaa aatgggagct
    16081 atctggctct catttcgata tggcccattt ataatgcata agttgcgaga atatgaactc
    16141 tccctggcaa ttgcggctga gaatttgaat ttctgtgcaa gccatgctga gccagcccag
    16201 gaaatcacta gagattccat catttcatat cgttcaaatt caagaatcgt tcaagcgctc
    16261 atatggactt caaatgatga ggccgaggaa ggctccgtaa ggaaatctct caaatcaatt
    16321 ttaggatctg aagatagagc aaggcattcg gtttggtttt ccggccatag gcctgtatca
    16381 ggacactttg cacttagggc agaagggcag cttgtacaga tccacaaccc agcattatgg
    16441 caggttgttt tggtggatgc tgagtccagg agagccgatt ttttcacaat tctgcccgat
    16501 caaccaaaac ccaaaaaggc agccagtctt gtccttgacc ctttggctcg ctaaaagtag
    16561 gatcaataca atatggcggt gcgaaagaaa aagcgtaacc aagcaaaaac gggttgtcta
    16621 atctggcttg gagcctttct cattcttcta attctattcc tggtcaaatt tgaggatatt
    16681 cgtacggctg ttgagaaaac aggctttata gatgctctca accatgctgt atccaagcaa
    16741 gattctgaac ctcaacagaa accgcaggaa actaatccgg aagaacctgc gcctgcaccg
    16801 acactaccat ctgcagcgcc acctgcggaa gaaccgcaga ttcaaagtcc aatactaccc
    16861 aaagaaacga ccacgaccac caaagcgcca aaagaagtcg agttgccttc tgctccgcta
    16921 tcagagtcag agccaatagc ctcttctccg ggagcatcta tctccaagct gtccaaagaa
    16981 gaacaaaaga cgaggacagc cgttctatat tttgtgtaca taggagatga tggctcgatt
    17041 tccagccaaa aagtgaaacg gacactggca atatcggaca gtcccctgca ggatactctt
    17101 gagtcgcttt tgaaagggcc aacggaatca gaattacggg cgaatcttct atcgttaatt
    17161 cctgcagaca caaaacttcg aagtgtcaat ataaaaggcg gtactgctat tttggatttt
    17221 aacgaagcat ttgcatataa tcgttatggt aaagaagggt atttagccca gcttaagcaa
    17281 atagtctata cgcttacaga atttcagaat atcaatgatg tccaattctt aatagagggg
    17341 aaatcccgtg cttttctttc cgaaggcatt gtcctcgata aaccctggtc tcgaactagt
    17401 ttctaaataa gctcgttgtc agtatgcatg agtgatgtta gaataaacaa agcatttcat
    17461 aatagaggag gagcctacga ataccacggc attgcatgat tggcatgtct cgcatggcgc
    17521 taagatggca ccttttgcgg gctacgatat gccgatcaat tatcctaccg gcgctatcga
    17581 agaacatctt attacgcggc ggggggttgg tctctttgat atagatcata tgggtcagtt
    17641 cgaaataacg ggtgatgccg cagagagttt tgtctctcat atgataagcg caagagtctc
    17701 ggatcttgtt gcgccaatgg ctcgatactc actcctgctt aatgaaaagg gcgggattat
    17761 tgatgatctc tttgtttata gattgcagaa ttcctggtgg atcgtcgtga atgcatcgaa
    17821 tcgagcggta gatcttgaat ggttccgatc acatgttcct tcgaatgtct ctgttgtcga
    17881 tcgttcggat gacacatata tgatcgctgt tcaaggtccc cgagccattg aactcttgga
    17941 taaaatatcc aatactccgg tttcgccgat cccgcgcttt tgctgggcaa atatctccat
    18001 aagaggtatt cctatcctct tcggtcgtac aggctatact ggtgaagatg gcggagagct
    18061 tttctttccc gcagaaaagg ccattgagct ttgggaatac cttcttgaat gcggagaaag
    18121 ttatggtatc gaaaccagac caattggact tgcggcaaga gacagtctgc gatttgaagc
    18181 gggattgcca cttcatgggc atgagattgg tgtcgatatc aaccccatcg aggcgggatt
    18241 cagatgggct tgcgacttcg aaaaagaatt cataggaaaa gatgctctgc tttctattat
    18301 agaacaaggt gtcagacgaa agcttgttgg tcttgaggtc tttggaggcg ttgctcggga
    18361 aggatatgaa gtcgctacgc cagatggtga cattatcggt cgctgcgttg caggtatgta
    18421 ttgcccgacg gtaaagaaat atgcggccaa tgcctttgtt attcctgact atgccaagat
    18481 aggtacaaag ctatcggtta tcatccgtgg ccagcctcgg tcggctgaag tcattaagcg
    18541 tcccttctat gtgcctgtat atcggcgcta aaactctagt acgcactaag cgtcttgcaa
    18601 tatataattt ctcttaaggg aggaaagtat ggcgatcgat aagaatgcta ggtaccttga
    18661 gtcacatgag tatgcaaagc cagaaggcga gctcatggta atagggattt ctgatcatgc
    18721 tcaagccgaa ctaggcgatg tcgtgtttat tgagtttccc gcagtgggaa aagagctcga
    18781 taagggagtc gtatttggaa caattgagtc agtaaaagca gcaagcgacc tctacatgcc
    18841 tatttcgggc aaggtggtgg aggtcaacga agaagtgaaa aatgaccctt ctcttgtaaa
    18901 taaagactgc tatggttctg gctggcttat aaaagtggct ccatccaaac tttcagaatt
    18961 cgagagcctt ctcgacgcgg aatcctatgg cgcatccata ggggaggtat gacatcgtgt
    19021 cctttgttcc gcatactgat ctagaaaggg ctgagatgct aaaagcgatc ggaatccctt
    19081 ccatagaaga tctgttctct gatattcctg agcatctgcg aatagaggat ctggcgcttc
    19141 ctgaatctat ctctgagatt gaagcgatcg atcagctaga gactttggca agtcgcaatg
    19201 tccagacaaa agatatggat tggtttctcg gagctggcgc atactatagc tatattccgg
    19261 cagttgtgcc tgccctcgca ggcagaggcg aatttttgac agcctacact ccctatcagc
    19321 cggaagtatc tcagggcaca ttgcaggcga tcttcgaata ccagagcatg acggcaaagc
    19381 tcttaggaat gtctgtagtc aatgcttctc attacgatgg agccacagcg ttggctgaag
    19441 cggtgctcat ggcctggaag gccagagaaa acagaaatcg aatcttcctg cccaaggatc
    19501 ttcatccgga gtattctcaa gtaatttcca cctactgtac ttctttcgat atagaattca
    19561 tcagatacga aggagatcct ggccaggtag caatagatga agacactgcg gcaatagtcg
    19621 tagcctatcc gggtttttct ggacaatttt acccagtcag caatgtttcg gataaggctc
    19681 acgaaaaagg agcgctctgc attgtgcaag ctgatccctt aatgtgcgca atcttcaaga
    19741 gccccggaga gcagggcgca gatatcgtaa cagccgaagg gcaatgcctt ggtaacccat
    19801 tgaactttgg gggtccttac ctgggaatga tgggcacgac agatgcactc ataaggcgca
    19861 tgccgggcag gatcgttggt cagacaaaag ataaattcgg caaacgcggt tttgtgctta
    19921 ccttgtccac aagagaacag catatcagaa gagaaaaggc ggtctcaaat atatgttcga
    19981 atcaaggctt gagcatgttg cagacctgca tctatctagc gacgctcggc aagaatgggc
    20041 tccagaaagt tgctagacta tcctacgata aagctcacta tgctgctcaa cttatcgact
    20101 ctattccagg atatcatgta gttccgaaac tgttttttag ggagttcctg gtcgaaacac
    20161 cagatgcggc tgccaagatc gtagaagcat tgaacaagaa gggtattgtg ccaggtttgg
    20221 ctctctcccg ctattatcct gagagaaaga atgagctctt gatttgcgtt acagaaatga
    20281 atacaaaagc tcaaatagaa aagctcgctg ttgcattgaa ggaggctgca aaatgaatga
    20341 gattaatatg cgcgacgagc ctttggtcta tgaattgtct aaggaaggaa gaattggttg
    20401 ttctcttcca aggtccgatg ttccgagcac aggaatgcct cctgagcttg ctcgcactga
    20461 attggatcta ccggaactcg atgagcttac ggtggttaga cattttacga gactctccca
    20521 aaagaatttt tccatcgata ctgagttcta tccacttggc tcgtgtacca tgaaatacaa
    20581 tcccaaagcc aacgaggttg ttgcttcgaa tcctggatgg aagaatcttc accctctcgc
    20641 agaagagcaa tatgcacagg gagccattga gcttctatgg agacttcagg agggacttcg
    20701 cgaagtgggt ggttttgccg ctgcttctct tcaacctgca gcaggtgccc atggtgagct
    20761 ttcaggcgta ctgatgattc atgccgcttt gaaagaactg ggagagacgg agcgaaaaaa
    20821 gatgctcata ccagattcag ctcacgggac aaaccccgca agctgcacta tggccggatt
    20881 tacaactcag accataccct cggggcctga tggtagtata gatatggaag cgcttcgcgc
    20941 tgctctcgat gatactacag ctggaataat gatcaccaat ccgaatactc ttgggctttt
    21001 tgagaggaac atagaggata tttgcagact tgtgcatgag gctggtggct ttgtatatgg
    21061 cgatggagca aacatgaacg ctcttacggg cgttttcaag ccgggcagat cgggcatcga
    21121 tgttatgcat ttcaatctac acaagacttt ttctactccg catggtggag gcggcccggg
    21181 tgcaggaatg gtagcagcca atgagacctt ggcgccatac ctgccgggcc ctatcgccat
    21241 caaggaagca tccggctatc gtcttaaaat gccggccaag agcataggca gggtaaaggc
    21301 cttccacggc aactttggcg tcctggtgcg tgcatactgc tacttgttga tgaccggtgc
    21361 ggatggactt cgtcaggttg ccgagaatgc agtgctaaat gcgaactacc ttaagtcgct
    21421 tttagagaat gactatcctg tcccttacaa aagacattgc atgcatgaat tcgttgccaa
    21481 gggaacgatt gccgctgatg tccacacatt ggatatagcg aagcgtctta tagactatgg
    21541 cttccatcct cctacaatct atttcccgct catcgtacag gaagccctga tgatcgagcc
    21601 aacagagacg gagtctaagg aaaccctaga tctttttgct gaggcgatga aaaaaatcgc
    21661 agaagaagcg agaacggatc cggagcttct gcatgatgct ccacatattg cgcccgtagc
    21721 tcgtctagat gaggtggctg cagcgcgaca tcccatactc tgttatcgca gctagtattt
    21781 ttatttcaat gcacgaatat caaaaagaga aagatttcat ccaagtcttt ctctttttta
    21841 ttgtttgttg gggctaaggg tctcttttta tctctcgcga taaatggtgg ttttgggaat
    21901 tgtgttgcct tataagccca gaatatcgaa catggcaaca ataccactgc ttcctcccgc
    21961 tgagattacg gattctccaa gaactgtgac attgtttatt gattttattc catagagatt
    22021 gtcttcttga gaattcaaag agccttggca aattgggtgc ccatcagtat ccagcgatat
    22081 gattccaagg ccatgattcc ctcctgcgac tagataggct ccgccagagt tcttttttac
    22141 aagcgcaagc gaattggctt cattcaaatc agaagaaaga ctgtaggtca ttgatagaga
    22201 ataagcagat agcggatctg acttttcgaa caaatgcaat ttcctggcag cgccgatcac
    22261 atagcatcgt tcattgccct catcaaaggc gatcgatttt gggtctccaa gagatgcaga
    22321 atttgagcca gatgtaaata tgatcggctc ggaaaaagag cagtcagacg atgaatatct
    22381 aatgaaaatt accatcttga tggtcgaaga acacgctgca atccagccat cgtctagtag
    22441 aatcatatct tgcatcccta atgaagaatg ctctgtctca gaaggatttt gctgagagcc
    22501 ttcagatgtt ctattgagcc aacctgttat agagaattcc tgttcgcaac atggttctcc
    22561 gcgaaagagc ttttcgatat ctaatacaag taactttcct gatgaatttg ctaggatgaa
    22621 tagccttgtg gaatcggttg taaacctcat tgcccttata ttctcaaatg tcggggctcc
    22681 gttctcgcca ctgataatat cgattttcct tgagatggaa ccagaatttt caagtctata
    22741 gaccctgatc cattttccgg cagtcgctgc gatagctatc atcgatccat ccattgatat
    22801 gcttgctata tccgggtttt tttcgctttt ttggataaat atcctatccc gccataggcg
    22861 tatccatccg atagaagaag ggatttggcg gttgttgcca tctaatgtag cctcctcgcc
    22921 atatggagaa tagaaaagat gcagagcact ggcctggtcg agcccggcta ccgctatcaa
    22981 tccagtaggg cttgttgcta ccgattttgc ctcatgcaaa ttaccagtac cggcattttc
    23041 aggatagatg ctgttgtgga gcgatggatt tagtgcagcc ttatagtcga tactctgtat
    23101 ccattcgatt tcgcttgatg agggtcctgt aagaagcgat gaactatgag agaatgcctg
    23161 cgaaatgcca gaattccctt cgctaagaag cacttccatg gatattcgca tttgagtatc
    23221 gggttgcttg tctggcgaaa agaaggtctt ataaccaggc agactttggt ctgcttctgc
    23281 ctgagtttcc tcgattattg ttccattctg gtaccattgg acatctagat cggcctctcc
    23341 ttgtgcaatt acaaaaggca cagagatggg cttatttcgg ttcaaatacc gctcaacaga
    23401 aattgcagca tcggccgata attccaattt tggagtgcta agcgaaatgg tggccgatgg
    23461 atccggaagg gatatactac atgagcctcg tgaagtttgt tctggcaaga ttagcactgt
    23521 tgtaatacag cccgctattg aggtggaaga agtatcttta acttgtattg caaggctgta
    23581 atttcctgat tccagttcat tgacctgata atccatctgt gtagccggaa catctgtcga
    23641 taaaggagct ggtccctgaa aagtgggatc tcctgggagg cctctgtatt cgagcgacaa
    23701 agagtatttc cagtcctgcg gaagtggctg atttggagat aatgctagct gcagagagcc
    23761 ttttccttga gctaagtgca tcattatctg tatgttggct ctctttcctg cttgagccag
    23821 gacttgcgtt tcttttttga ctattatagc cccgtcagca gtaaggcctt cggctagtag
    23881 agaccaattg ccagccttga ggctcagctc cgcgccggtc ttaccgatag atttggtgat
    23941 gacttctcca ttctcactcg tgaatgtcaa acgataggat gctatattcc atccggattc
    24001 agggcctata agccttgatt gagttttcct ggtgtttcct tctgtcaggt tacctggcaa
    24061 gacacatcgt atgtatacat atccattacc cttaggaatt cctgaagggc aagctatgaa
    24121 caagaaagct gtaataaaga taactgctcc aagcgataag aatattctgc gctccgtcat
    24181 attctcctcc taaagacaga gtcgcgcaaa aactggctca ttgcttcagg aattatgtcc
    24241 atatctgagc atcggagtgt ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24361 nncatcggag tgtttgccaa acccgccctg cccgaggcta cagatgaaaa gcagccttga
    24421 agccgcattg cagcatccta gcctcgcatc atataatgaa tggctagaac attgagatgg
    24481 gaggcggatt gtgaaaatac tgtttatttc aagcgagttg acaccatttg caaaaagcgg
    24541 tggactcggt gatgcagtat cttcgctcgc agctgcgctt tctaagttgg gtcacgatgt
    24601 gcgagtgctg cttccacgct actacttcat atcaaagaac attctgagaa ggataaatgg
    24661 tcttctggag atcgattctg cacgagggcg ccatcgggca tttctttata caaccaatct
    24721 cccaaactcc caggttaagg tttattttct ggaatgcgaa agtctatatg gtcgaaatgg
    24781 aatttatggc tattcctcga accaggaatt tccagataat cctgagcgtt tttcggtttt
    24841 gtccaggtct gcctttgcgg tctgccgcaa ccttggatgg attccagata tcatgcaggc
    24901 gcatgattgg ccaacagcgc cggttcctgt ttaccttaat actatagaga agaataccga
    24961 gttttcaggg accgccggtg tcttgaccat ccataatttg ggttatcagg gaatctatgg
    25021 tagagatcat tttgccgagc ttggcttagg ttgggaatat tattatggag ctggttttga
    25081 atattatggc aacatcaatt ttctcaaggc ggggatagtc tctgccgagt gcatcgcaac
    25141 cgtttcgcct acttacgcta tggaaatcca aactccaagc ggtggttttg gactcgacag
    25201 cctgttgcgg cagcgaagca gagacctggt aggcattcta aatggcatcg atacggaaat
    25261 atggaatcct tcgaaggatc cctatctgcc ggcacactat tccgttgagg acatcgcagg
    25321 aaaagctatc tgcaaggaat cgctccaaag agagctaggc cttcccattg agcctgaagt
    25381 gccgctgata ggcatggtaa cgaggctagt ggagcaaaaa gggatttcgg aagtatttgg
    25441 tcgaacatac ggatgtatgc gcaagatact ggagacgatc catgttcagg tcgcggtgct
    25501 tggatccgga gatgcttggg cagaagaggc tattgtgaat ttttccgccc agtatccgca
    25561 attcaaaggt gtcattggat acaacgaaag gcttgcacat ctcattgagg cggggagtga
    25621 ttttttcctc atgccaagca gatatgaacc ctgtggactg aaccagatgt attcgctcgt
    25681 atatgggact ttgccgattg tccacaggac aggtggttta gccgacaccg tggtcaatta
    25741 taatcaaaat ttgggcgatg gcacaggttt tatgttcgat gatttaaccc cgcaagcggt
    25801 atacgatact gtgggctggg caatgtgggc gtggtataac aaacctgagc atataaagac
    25861 tatgcgcgag cgagcaatga aacaggattt ttcatggaat cgttcagccg aagaatatat
    25921 aaggctttat cagcatgcct tagccataag aaaggccagg tgagcattat aaagaaggct
    25981 ctacggataa tttaaaagga atacattgat gtctgaaact aaaatactgg attacaacaa
    26041 gcaagaggtg agagatcagc ttctgcgtgc tctgcgcagc catacaaagg aatcgacgag
    26101 cgcagatctt gctcgagcaa cgggcttgcc tctggcacag ataaatgccg aaatgcctgc
    26161 tatttccgat gaatatagag gcagaattcg ggtatcggag agaggtgatc tcctgtattc
    26221 ctttcccaat ggatttaaga gcagatacaa gggctttggg ccttctctgc gaagattgtg
    26281 gaagaccatt acgaggggag ccgtaaaaac aggaaaatta ctttttaaag tgtggattct
    26341 ggtgactctc tttggctact tctttctttt tctagccctt gctctaattg cactctttgc
    26401 ctcggtcatg atgcgagcgg gaggctcgag cgatagtaat gatcggcggg gaggcggtgg
    26461 acttggtggt ttatggctca ctactaatct ctttgattct atgattcgac tctggttcta
    26521 tagtgagcta ttcaaaggac cagagaccag atatcgaggc accagagcta aagcccagcg
    26581 gaaacctcta tataaggctg tattttcgca tgttttcgga gatggtgatc caaatgcaga
    26641 atgggatacc atcttgaaaa aggcatttgt ggccttcgtt cagacccata aaggtgtaat
    26701 tacattgccc gaattcatgg caatatctgg gctaaagcca gaggaggccg aggattgcat
    26761 aacaagatac atggtggaat tcgaaggcag ccctgaggtt acagataatg gagcactcta
    26821 ctttttcttt ccttcattgc ttgcaaaggc cactacggtg tccggtgccg cggcctccac
    26881 gttcccgatt aaaaagctcc atcgcttttc ttcgaaccca gaaaaattgg atcgaacatt
    26941 ccgctgggta aacatattca accttgtatt tggctcatat tatctatgga atgccataaa
    27001 cctaggtact gcaattataa ttcagactcc agagggacaa gggttaaaag gagggctaag
    27061 ctttttgtat tcttacacag tctatttatt ttctgtgttg ggggcgaacc agcccgcggt
    27121 tcttgtagga tgggtgctgg gcgttgcacc gctggctttt tctctactct tttttgggat
    27181 accgatcgtt cgatctcagc gaatcaagcg cgaaaatgag acgataaaac gcgaaaatat
    27241 gagaagagtt ctttattcat ctgtgattgc tgaccccaac tactttgatc ctcagtctct
    27301 gcaattgcca gccaatgaag ttcgtcctga aaaagccgat gcagtgctgg aagagacaca
    27361 gcatcttgca gcctggagca ggggggaaat cagccctgaa ggtaagtgga gctttggtga
    27421 tattgaattc atcgaacggg aagccgaacg agtccgcgca tcgatcgaag agagagatta
    27481 ttctccacag aagacggtct tcgatacaga ttctccaata tagtagtaat cttgagattt
    27541 agcacaatga caagagctcg taatagtacc gcaatgcgtg ggttcattgc ggccatgatc
    27601 tcagccatca gcttttctac gcttggcatt tttgccgaat tgctctattc gcatgggttt
    27661 tccgtattat gtgtgcttgc atggcgtttt ctcgtagcag cccttttttt atggctcgta
    27721 gtatatattc gtgaatggtt ccagtcgaag gatattccag ctgaaaaaga tgcaacaaaa
    27781 aagatcgctg atcatgagca gcggcgaaaa tacatcccac gcgtcttttt gcttggtctc
    27841 gttggtttct cgccacaagc tgggctcttc ctcttcacgg ttaaaatcct ggaccccggt
    27901 attgcaagct tgttgatgta tctatatcct tcttttgtat ttctaatcct attcgtttgg
    27961 cgacatcaac ggcccaagcg catccaaatc cttgctcttg cgctttcttt tctgggatgc
    28021 atactcactt tttggaaggc aggatcatat ccgtttatgg gtcttgcatt ggggattttg
    28081 gtagcggtcg cttatgctgt ttatctcgtt attgtagagc gtgttcttgt tggagtggac
    28141 tctctatggg ctactgctat aattatgacg gcagccgcgc ttgtatacct ctgtcttgct
    28201 cttctacaag gtacttttat cattccttct tctaccgagc aatttatcat agttctttgt
    28261 attgcaatta ttgcttctgt cttgcccatt gtggcgcttt tcgttgcgat gaagaatcta
    28321 ggtgctagag atacttctat tatcagcact attgagccag tattcacaaa tattttctct
    28381 atattgctat ttggagaaaa cttgacgatt cagagaatct tagggggtgt ttctattctt
    28441 gcaggggtct tgattctcaa cagatggggt tatgcatctc ataaaaagga ttctgcggca
    28501 gatctcagcg ggccaagttc gcccttatcg aagtccccag gcacagagga aggaaaattg
    28561 taatccggag agatgagcaa gccatgttca agacagtata gaaaaacctc ttcatgattc
    28621 tcccttggat agtttgggaa aagccatgga ccagaacggg aaaagagtac tcctccatat
    28681 cggtcgaagc tttcccatac aggctctagt cctccagaaa ttacaccttc ttcttcagat
    28741 tgcatcttag atagagcata gatcgaagtc atgcctttta tgccctcgat aagctcttgt
    28801 tcaattacag gacttgagga catcttgctt gcgagaattc cgatcgatag aaaatcagga
    28861 ataggcagga caatactaat aatatccgaa agctcgtcat ctctttttag atatttatca
    28921 aagaggaatt tgcaagtgcc aatgcttgga gtggaagaag caagcccttc cagattttgc
    28981 agtaatttga ttgccttttc ttgtgagcct agaattttga tattgcgata ttccggtaaa
    29041 agtctatgca gcattttttc gatacggcta ttccatattc ccggtaccgc tgaaaaaagc
    29101 cccctgtcca tggaactttt tgcaattgag acaaggccgc gtggactata acctagaagt
    29161 gtcctgccat agtctgacca aagactgaga attctcgaac catttcgagc atacattcgc
    29221 cagcctcgca tccggtttat ttcgggtaga tgcgagagaa gagaatcggt gttgggttgc
    29281 aaaatgcttt cttcactggc tttgtcgcgc ataccggaag gttcccctct tattctcgac
    29341 ttcaattctt gattctctcc aatttttttg tgtattttgg gaagaattgc gcagtctttc
    29401 ctgtctcaaa ctggactatt acaagaggac ctgcactctg agacggtgtt acttttataa
    29461 cggttccatt gccgtattca tcatgataga cagacatacc cgctgtccat tcggatttct
    29521 tggtcgatgt atcggattga gaagaaacag caaccgtgga tgatgaacga tattgtgaag
    29581 ggactgctct catcttattt ctggaatcgc cccacatctt atatagagaa gggtcgattt
    29641 ccgataagaa ttgactcggc atggtctcaa agagctttcc atggattcgg cgccaacgac
    29701 atgaagtcag ataaagctgg ttttttgccc ttgtgacaga gacatagaag aggcgccttt
    29761 gctcttctat gtcatcaccc tcttcgtcgt tccgtgggaa gagcccttgt tcgagccctg
    29821 taactattac tatgggaaat tcgaggcctt tcgtgttgtg catggtgatc aatgtcaccg
    29881 catctgcatt gtcttgattt ttctgtcgag attgatcgag catgacatca tccaggaatt
    29941 ccagaaggcc ttccgaagaa agcggatggt ctgatgctgc gttgacgagt tcttctatgt
    30001 tctctttttt atgggtactc gatattctat ctttgctggc atagaactcg agaagaccgc
    30061 ttttttgagc aaaatcggaa ataagcgacc ccaggtcgcc tggatttttt tgatcagcca
    30121 agtctctatg aagtgaagcc ttagcttctt ctataaggct gagaaattct ttgagacctt
    30181 cgcttccttt acgagaaatg cttgcaattg attggcgcga agcttctatc agatcaaggc
    30241 cattatggaa cgcaaaagag ataatcttat ccagagatga agcaccaatt cctcttgaag
    30301 gcttatttac gacgcgtttg aaggaaatct catctcttgg atttgcaagg atagccaaaa
    30361 acgctagcat atccttgatc tcctcgcgtt catagaatcg gacagcacca acgattcgat
    30421 aaggaatatc aaattgtggg aaaaggcgtt caaagcctag agactgggca tttgttcgat
    30481 agagtatagc gatgtctttc cattcgcctt catttttgac atgctggcgc gctatatgag
    30541 cacagaactc aatttcttga tcctggtcat cgagaaatgc gatttttggc ttatttcctc
    30601 ctggtctggt agctagcaga ttctttccaa gcctgttttc gttgtgtact actatgctgt
    30661 gggcgagatc caaaatagct tgatgcgatc tatagttacg ctcaagcctg attatccttg
    30721 tatctgcaaa gactttagga aaattcagta tattcttgac ttctgcgcct ctgaaacggt
    30781 atatagactg gtcgtcatcc ccaactacac aaagcatcga atcagggcca gcaagctgct
    30841 gcaaaagcct aaattgagct atgttcgaat cctgatattc atcgacaagg attactctaa
    30901 agcgctggcg tgtgcgattt cgaattgttt catctttctc gagcaacaat gcgggcatca
    30961 agattaggtc gccaaaatcc acattacctg tggaacgtag cctatcttca taagctgaat
    31021 agatgcgtct gaattccgga tgtctgctta taaaatccag gttcggggaa tcaggcgaaa
    31081 ggcaataatc tttagccttt gcgatcagaa aagcataatc gccacaatcg cgctttgtaa
    31141 aagcaggaaa tatcgaatga acaagctcgg ccgaatcatc ttcatcatag atggaaaatt
    31201 cgcttcgcaa atcgaaggca ttggcgttgc gcctcatgaa ccatgctcca aaggaatgga
    31261 aagtccttat tatcgctctt tcacatgaag gctcgattgc catagcccgc tcgcgcattt
    31321 catttgcagc cttgtttgta aatgttactg caagaatgga ttcaggcaaa aacctgcttt
    31381 gtttgaccag gtgcgctatc ttggttgtga taactctggt tttgcctgtt cctgcacccg
    31441 caaggatgag aagacggttt ccttcatagc agactgcttc aagctgttct gggtttaatc
    31501 cttccagata ctcaggaatc tgtcctaata tcatggtgtc gtagtatact ctaattctgc
    31561 attgaaatcg acaccattga cagaaacaat gttcgccttc gctattgtgc caggttttag
    31621 agatgcattt attatagtta caccatcgat atctggggct tgcatccagc cgcgtccgaa
    31681 gcacagctca tcatgatcga atgcctcttc tatgatgaag gtatcgctaa ctccgataaa
    31741 gcgctgcaat ctgttggcgg ttattgcaat ttgttcattt tctagaattg cttttctttt
    31801 ccttgcgatg gattttggga tgcgagcctt catgtcataa gcgggtgtat tctcttcaag
    31861 cgaatatgta aacacaccaa cccaatcgat ctctgcggtc ttttggaaat tgagcagggc
    31921 agcaaaatcc tcttcggtct ctccaggaaa tccaacaaga aaagtggaac gaatcataga
    31981 catgggcagc tcacttctta ttctgtatat tagattcaaa tattctgtag cagaaccatg
    32041 gcgattcatt ttttggagaa ttggagtcga agcatgctga aaaggcagat cgaaataagg
    32101 cgagaagcga gaatcatgcg ctatgatctt taatatctca gaaggaaaat ggtcaggatg
    32161 aatatagaga aaccgtattc taaaatctgc ctctatgttc gatagagctt ccatcaagtc
    32221 cataagcaag catcgacctg acaaatcctt cccgtaattg ccaagatctt ggccaattag
    32281 gacaagctca aacatgcctt gtgcaatgag attccgagct tccaatacga tatcttcgat
    32341 gctccttgag cgaagctcac ccctaatcaa tggaatcgcg cagtaactgc aataattcga
    32401 acatccttct gtgattttga tatgcgctgt tcccggataa tcgaaaaaga ctattcgtgg
    32461 atagtatggg ctgggcattt tcttattctg ggcagtggca agcacaattc gttctcccct
    32521 tagaatctta gtcaccgcct ctgggattat ggagagattt ccatttccca gcacgccatc
    32581 tgcttctggc aggccattaa atagttcttc aggatagcgt tgcgagaggc aaccgattgc
    32641 gattactttt tttttcggca atctagtctt tgcttcgact attgccgcta tggattcttt
    32701 tttggcatct tcaatgaagc cgcatgtatt tataataata gcatcggcct catcgggtga
    32761 gcttgcggct agaaaccctg cattgatgag gcgcgaactt atttcttccc catcgacttg
    32821 attttttgca cagccatgtt gatctacgaa aaaggtaggc atataagatt tcctcaagat
    32881 cttgcatgat atacatagaa taaaaggatt attgacagca agagctttaa tccacctgag
    32941 ttgctatcat tgcccatcca ctttgcgaat tcgaccagga caggcgttga accgcgattt
    33001 ctccaggccc tcccatcaca agctccacgg acttacctgc tgattgaaat acattcactt
    33061 ttacagcttg agcatttgcc gtccaaaaag ttatggtatt agatgcgttt accgttatgg
    33121 attctccctt gcggtagtat ttttcgaccc attcttttct gtcggcttcg taacggaaaa
    33181 agaccggcgc tatgaaactc acattaacat aaaaaggatg gggtccaccg gtgcttctga
    33241 aaagtactgt cgaagatgtc tgtgtcctgt ttgccccacc tgcttggcct tgtgtcgaat
    33301 cttgggaaag ctgagctttt cccggtgttg acggcacaat gatatccaat gaattctcag
    33361 catcggaaag ggcttcttcg acatcgaaat gaaccatagc cccattctga gatttatccg
    33421 cctcataatc gctcaccgca ataacgagcc gttctctatc ggcggaaggg tccactactg
    33481 ttttttcatt caatccaacc gctaagcttc caaatggagt attgaatgag accttatctt
    33541 tgatggattc gagggtaata gagatgacat ctccgctgag agggatatat accttgtcct
    33601 gcggatagag gcgctgctcg aaaggcagac cttcggcgcg atactcggtt ggaactcgag
    33661 cggcgccagg agatattctg agtctgggta taatgctgac agctgcgata ataagcacta
    33721 tagcgacaaa aacgaatggc aatactctgc ctacccgaat attggcagta ccgattttct
    33781 tctttggttg aaaatcgata agggcagctt tttccaaagt actatccact tgaatggagg
    33841 cttttggctc tgctttgatg gtttctggtt cttctctctt aatggatttt tcttttggtt
    33901 ttctcttcgc atggaaggtt tgcttagtct cttttgcgga tttttcgact tcggatgaaa
    33961 taaaggattt ctgaggcaaa gtttcttctt ttttgctatc ttcaacaggc aaaggtaact
    34021 cagtgctttc agatttttcg acttcggctt gtcttgtgct ctcatttgat cgataaatct
    34081 ccaccatggc cgatgcatcg aggcctagga actcggcata atttcttata aagccgagac
    34141 tgtatacctc accggggaaa tcactgaaat catcactctc gattccttgt aaaaagtgct
    34201 tgctgatatt ggtctcgtcg gctacttgct ccagcgtcaa cccttttcca aggcgagctt
    34261 cacggaataa atcaccgatc ttacccatca cttctccccg cgtccttggc ttttcttact
    34321 gaaacagaaa attgctgtag acatttgcat tcgcaggcgc attatagata aatttgttgt
    34381 cgctgatgcc tcggttcaat tgtatattca caaaatcaaa ggtaatctta tcattcgaga
    34441 cggtccagcc ttctagtctc cgaataagca tagtatcggc gcttaccgaa atccgtatgt
    34501 tcttgtatcc ctcagacacg ctttttctag acagcatgag gcgataaacc agctcatccg
    34561 agcccgggtc cagtggttcg ggattagggc tttgctccca tcctatggta taatttcgcc
    34621 taagcagcga gagaccttct tttgaagcga gagtcgctgc tccaacggaa atgccctgat
    34681 cggtcgaatc ctggcgaagc actgctctat attgaggaag ataaaccagg agcgttttac
    34741 catcgaacac aatgacctga tcggctggct gagaaaaatc aatgcgcaat agagaaggcg
    34801 atttgaagag caaagtccct gccatcggtt gatgaccggc agttatatgc acatccgctt
    34861 gataatcagt taccattgca tatttttcgg ctaattgcga aaagaactga tctgcggtaa
    34921 gcaatacctg ggcactcaat gtgcttgctc caagaaaaca agcgaaaata gaagcaataa
    34981 tggtaatatg tttccttttc acaagcgttg ctccaactgc agagttatag acggagtatc
    35041 ggtaatcgaa gggggaccaa aatactggat gctgccagga atccgatagt ctgatgagag
    35101 agcccactta tctcgcattg cagagaatgc tttgaatggc tttccctcga gttccaccag
    35161 cgcctttttg atgacaggtt tttcttccgc gtgccggtgc tctatgttca tcaatgagac
    35221 aataggtatt cctccaggaa gccaatcctt tggagcggct gcaagatttc ttatagcagc
    35281 aatatagccc gtcaatccgt gcagaatgag gagacatgca tcatatccca gagaataaca
    35341 ataatccgca tcgaaattgg tgggaaaagc gcagcggcct tcgtacccaa agaagtgcgt
    35401 ctggtaatcg aattttgcct tgtatgctcc aacagcagtc atttcttgaa gtttgcgaga
    35461 agtcatctca gcgagtagtt tttcggtttc aattctggag acttgtacat ttccgtgagg
    35521 gtctctatcc cagcaaagtt gggtttgaat cgattctgga aggctagaga acaacgaaat
    35581 acactctgaa ggcaaatgcg ataggatata ggatctctgt gcttctcctt tgggcaagga
    35641 ctcatattca tcttcggatg ttgcgataat gtcgttgatc tgagcaatca gtgtcttgat
    35701 ttccggaatg aattctataa ggccttctgg cacgactacg accccgaaat tctcgccttt
    35761 tgccgatcgc gcagctattg tgtttgctat gatttcgaca acttgagaca ggctcatctt
    35821 ctttgcatcg acctcttcgg atatgagagc cacatttggc cttgtttgaa aggcacattc
    35881 cagagtgata tgcgaagccg atcgccccat cagcttgaca aaatgccaat acttgcgacc
    35941 cgacgaagca tctttgcaga tattgccaat cagctcagaa tataccctgg ttgcggtgtc
    36001 gaagccgaaa gaagtttcga tccattcatt cttgaggtct ccatcgatgg tcttagggac
    36061 accgactact gtgattggag ctcccttttg gaaaaaatac tccgaaagaa gtgcggcgtt
    36121 agtattggaa tcatcccctc cgattataac aagggcgtcg agccccagct ttattgcatt
    36181 ctccaaggat tgttcgaact gctctgaagt ctcgatcttg gttctccctg tacctatcat
    36241 atcgaatccg cctgtattcc tatactgatc gataagctca gagctgagtt caacatattg
    36301 gccttttata aggcctgatg gcccactctt gaatccaaga agcctggatt gaggatttgc
    36361 ttttatcaac gcatcgaaaa gacctgctat gacattatgc ccgcctggag ctggtccacc
    36421 ggaaagaatg accccgacag taatagcttt atcttctaaa gccccctcgc cctttacgaa
    36481 ttttatcgcg ggcttgccat aactgttagg gaagaacttc tttagcactt ccctatctct
    36541 ctcaggttct atggtttcgg tagggatagt ctttatgctt tggattgaat ctagaaatgt
    36601 ctcaggcatt tttggaacat aagaatacct gccagcatgt agcggtgatg tttcgaacat
    36661 ctgaatatac ctctcgcata gaatatcgaa gacattctaa caatttggct tttgaatgaa
    36721 aagggcatta atgacaaatt tcttcttcaa tgacgaataa agcatgccga taaattaatt
    36781 ggaacttcct atggttagac gaattctaaa agcctccata ttctttttgc tttttggttt
    36841 gcttctcaca atcagcggtg cagaacagga atatcatgtt attcaggcag gcgaaacgct
    36901 atactcgatt gccaagagct attctgtgcc atatgaaacg cttgccagta tcaatagcat
    36961 aactgatccg tccaagattc gtccgggagt agttctccta atcccccgag tccacattgt
    37021 tgcaaaaggt gagacatatt atggtctttc taagcaatat ggtatctcaa ttcaagagtt
    37081 gaaatcagcc aacaacctct ccgacatcta tgtgctcaaa gtcggagatg tcctaatcat
    37141 cccggaaaaa ggagtcattt ctgtacagac agttcaagac tcgcctcctt tagattccgt
    37201 tgtagattcc gttgccatga gccagcccgt tgcttcagct tccactcagc agaattcatc
    37261 ttcccctgca aataccgcta ttgtcccatc caattctgtt tcaaccacat cttcaacttc
    37321 tcaggccgtt cttcctccaa attcccctac aacatcatcg tctgcagctt cacaatctac
    37381 aaatccaccg gctcagactt ccgccccaac caaggccgat agtcccaaaa ctagcgtatc
    37441 aggtaaacgc attccagatg caatcgtaaa ctggccagta gatggaaaag ctcagtatat
    37501 gagcgggaag ctcgaaggcg tgatgtttca gaccgcgcga ggtgcgtcgg taaaggctgt
    37561 agcttcaggt acggtagtat ctgcagggcc atctcgtggt tatggcgaag tggtgttcgt
    37621 acaatctaaa gctggttttg tctatgtata tgcaggcaat gagtctattc ttgtaaaaac
    37681 cggagaaatt atagaacccg gaaaggccat tgcaaaggtt ggtgtagatg caaatgatgg
    37741 caatccgata gcatatttct ttgtctttcg taatggccaa cctgtcgacc cttcgcttgc
    37801 tccacgagac tgacaataaa gcactattag cactattgac tttcatcttg tgcgttcaaa
    37861 tacaataagg taaaattcta ttggttttga ttgccgaatt agccaattcg acaaggaatg
    37921 gatatgggtg actacttgga ttgcgataaa gcaagcattt ctaagttacc ctcaagaaaa
    37981 ggctctcaag aaaaagcaga gcaggaatcc gatccgctga gcttatatct tagacaaatt
    38041 gccaggtttc cacttctcga tgcaaaacaa gagcaagaaa tcggtgtcaa gcttgaggag
    38101 cttggccttt tgctaaagaa ctatgataaa gaaataacta aagaccctca aaatataccg
    38161 cttttggaga tgaggagaaa cacacttgaa aagataagag cgtcaaagga agttctgata
    38221 acttctaatc ttcgcttggt agtttccata gccaagggat atcgaatgag aggcgtaagt
    38281 ctcttagatc tcatcgatga aggcaatatt ggcctcatcg aagcagtaaa ccgtttcgat
    38341 tataaaaaag ggtatcgttt ctcaacctat gcaagttggt ggataaggca ggccataatc
    38401 aagtgtcttg tggatcaaag caggatgata cgtcttccga ttcacatgtt gaacacgatc
    38461 cgccaatgtt atgcatcggc taaaggcctt gtccaggaac ttggccgtga gcccaactcc
    38521 atagaaatag ccgaaagatc cggtatagct ttgagcaagg tcgagagtgc tttactcctg
    38581 gcgctcggaa catccagcct tgatctatgt acaaaggaga accagaacac aagcccggta
    38641 aacaatctca aggatgaagg atcgtccgat ccttatagcg atgcattcct ggttacctta
    38701 agagaattgc tgagttatgt catggattct ctttcggagc gggaacaagc tgttctagag
    38761 cttagatatg gtctgaatgg cgaagcgcca aaaactcttg aggcaacggg cttagctctc
    38821 ggaataactc gtgagagagt gcgccagatt caagaaagag ctcttgacaa gatgaggaat
    38881 agacgggaac tttcagactc aatccagtga atatgacctt gaagccattc cataaaggat
    38941 tgatatcctt gctataagca gcaaaagacc tacaagccga tggagtccta gcttgtaggt
    39001 ctggatatgt taaatcagta tgccatcttg ataattagca tcatgatcat tttatttgcg
    39061 ttctagtatg gcaagaatct cctgatatcc ttttgcttgc gcaatatctt ttggagtctc
    39121 tcctttgcgg ttgtgcgcat ttctatccgc tcccagagac agcaattccg acacaacatc
    39181 cgcattattc tctcttaccg catagtgaag tattgattcg cctaaaaaat ccgcgctgtt
    39241 cgtgccagct gccgagaaaa gtttcatcct ggtgcctgaa tccattccaa agatcacaga
    39301 aagaggtatt ttattgtcgg cattcgatac aaagagagat gatcctgctt gtattagcac
    39361 caccgctgcg ttaagatcac cctttttaag agcgagcata agaggagtat ctccttttga
    39421 gtttctcgca ttaagattgg ctccatgttt gagtgcttgt gtaagagctt cctgcccttg
    39481 tccactctta actagtatat gcagaacaca atctccgctg gaatcgggct tgtttataag
    39541 cttatctgta tagaattcgg gagttatctg tggattcttc gaaagtgata atttaaacgg
    39601 agagagttct tgagcattca gagcatgaat atcggcatcg tttaccaaga acaatcttat
    39661 caatgcggca tattcaccac ttacggcatg atatagaggt gtggatccaa ggtagtcacg
    39721 cgtatcgatg acggccttag ccttgataag ctcacttgcg gcatcatagc gatcattggc
    39781 cgctgcggtt atgagtgggg taaaaccgcg actgtcttga atctcggtct gtgcaccttg
    39841 agctgtaagt agctggataa tcatggcgct gttgttcttg ctggcttgat gcaatgctgt
    39901 ctcgccagaa taattctgat tattcactgg agagcctgct gcaaggagca tctgagttgc
    39961 tttgaaagct ccattcaata ccgcgctttg gagagggata tcgccattct tatttgctgc
    40021 attcaatgaa gaccccgcgc ttaccagata acgaattgca tcagtagcat cgtatttcgc
    40081 agccgtatga agtggaatct ctcctgcatt attgagtgct tcgatatttg ctcccttttt
    40141 gacgaggtaa gaaattcctt cgggaagatt cttgcgggca gcatggtgga ctacagaatc
    40201 tccatttgca tccgtagcgg ttatcacatt tggcctgaaa aaccattcca aaggtgcggg
    40261 ttttgcggtg agtgcgagaa acaggggatt ctcttggacc gcgttatatt gaaatatgtc
    40321 tgcattggca tccaatagca cgataccctg ctgagcatag ttttgtctga cagaaatatg
    40381 caaagcagta tcttgggctt tgttcttagc gttcgggtca gctcccttag agagtatcag
    40441 cttgagaaca tccgggctag ctttaagcga tacagcagtc atgagaacac tgttagagtt
    40501 atcatcgctc tgattcacat tagatggcag tacgatattg tccacagcgg tatagccttt
    40561 actcaatgca atcttgagag cattttcgcc tgcttggttc atcttgaaga tttccgcatt
    40621 agcttcgatt agaggccttg catattcata atgctcggtc ctggcgcata taataagagg
    40681 tgtgtctcct ttggaattct gtgcatttac aggggcgcct gcttgaataa gggtttcgac
    40741 tatttcggga gcatataact gcgctactgc cttatggagt ggcgtatttc cttggatatc
    40801 cattagtgca gggttggcct taaatgctaa gagaagtttg accatttcaa gacgctgtgt
    40861 agctggaacg gtgagatgca gaggcgtgct ctgattagca tcgagaatat ctggatttgc
    40921 attatgagag agcagaagtc ttgcaccctc gacatatccc atctttaccg ccaggtgaag
    40981 ggatgcatct ccatttaaat tcttgagatt gggatcgaca ttattgagca atagaaaaag
    41041 cgcaaaacca tgttgatatc gggagatagc ctcatgcaaa ggcgtgttgc cattagcaaa
    41101 gcgggcagca ccataatcag gcctccgcac ggtatccgca aaccacgaaa actctgggaa
    41161 agaaggattt gcccccttta gaatcagctt ctcggctatc tgggctgctt ccagtttctc
    41221 cggataaagg agagcgagat cgagggcggt cctgtctgcc ttatctttta tctgtacaag
    41281 ggcacctttg gatagaagta ggtcgacttc ttttggcatt agtgcatcgg aagcatggtg
    41341 cagaatggtt cttccatcgg gaccggtgtt attgatattt cgagagttga acattgcatc
    41401 gagaaggcta atgtctgagt gtagcgctac cagggctgga gaagtcccgc ccacatctgt
    41461 gataaaagga tcagcccctc tatcgatgag catctttacc attgctgtct gttttctgtc
    41521 cactgcatat cgtaaagctg tttttccaga gagatcctga ttatcgggtt tagcacccag
    41581 cacaattaga atctctgcta tctgagcatt gccttgggtt actgcgatat gaagagggta
    41641 aagtccgata tagggttctt tatttgaagg tttgttcaat tcctcttggt ttgaatagaa
    41701 cttacgtata ccttcaaaat caccctggat tactagatcg tttagagtga cctctttatt
    41761 ttttgcaggg gaggtcgcgc aacctccaag cagaaatagc acggcaataa tcgcggagaa
    41821 aaatcgtcct accttaaaag atcgtctttg cattctcaat atctcccttc aagaatatcg
    41881 gcatcattaa tacaaatccc tagtcttcac aatttatatc gcaaaaagtt gataatttca
    41941 taaggttgct tgcatttagg aaccaagtat gcttccatat atatgatata gtttatttta
    42001 agagaggcca tcatgaaaat tggatccttc atgccgattc agtctatctg gcgaaccatc
    42061 atagaacagc tgagagaaga atttcctgat cacacatggt tggaagggct cacctcagat
    42121 tcctccgaaa taggttcgct ggatctaatc attgctgggc gtattccagc cgatgttttc
    42181 aaagcagcga caaaccttaa agccctgttt cagccattta cggggataaa tcatctgcct
    42241 tctcaattga tgttagagcg caatattcag gtatataatg ttcattccaa tgccttcgat
    42301 gtcgccgaaa aagctcttgc cctaactctt gctttttatg gaagggtcat cgagtaccac
    42361 aatgacctca aaaatgagat atggcatggt ttttgggtta atcgaggagc agaagacaac
    42421 tgggatactc tatatggtcg gaattgttcg attctgggga caggagccat tggtcaggag
    42481 ctggctggtc ttctcaaggc atttcattgc acggtatatg gctggcgcag gacaaaagac
    42541 gccaaaaaac cagctcattt cgattccata gtgcccactt tgcaggaagc catcgatttt
    42601 gccgagatta tcttcatagc gcttccggct actaaagaga ccgagaatct tatctcaaaa
    42661 gagatgctca tgaacatgaa ggggaaattc ctggtcaatg tgggcagagg gtcgatcgtg
    42721 aacgaagaag gcttgtacga agctctaaag aatggagtgc tgaaaggtgc cgccatcgat
    42781 acatggtata cataccctgc cacaggtgta gtgggtgcgc cttcgcgtta tcctatacat
    42841 aaactttcca atgtggtgct ttccccccat gtgggaggat cgacaaatcg agcctcggct
    42901 aaatcgatcg aagacacagt ggctaacatt agatcttacc tcaggacagg gcgcggtatc
    42961 tggcaggctg acctttctaa gatgtattga ggccttatag aacagaatta taaagacaat
    43021 cagcctggta tctcagaaga gtccttcagg tgcctcaatc aacatatgcg gaaaatcagc
    43081 gtagattgat atgagttcgt aaagtcgatc aaaaggaaga gataatttat cgaaaatcac
    43141 ataaggcaga agctccgcaa ggcgatccga attgtagcca ccctttccgc ttaggatcac
    43201 cattgtctcc atgtcttgag cttcttcgag ggctcgatac gcaagggtaa agttcttggt
    43261 agcaaagaat attctggttt gataactagt ctgcggatcg ggggaaaatg agttcatgag
    43321 ctcataatag tgagcagagt tcatgaacgc atccctaatt ctctcaacaa gtttctcgga
    43381 aggtattctt gtctcgcttg taagcacgat ttgatagagt gtgtagggat tgtctttacg
    43441 taggtctctg gctgctctga ctactcggga cagtatttcg gggtcgtctg catcgaccag
    43501 aagagtgatg gagtttgaca gtttttccgg attgagacgc atccaatcta tattctcggg
    43561 ctttcggaga tcgatgaatg agacgaatcc ttctttaaaa agttgaaaat gaggtgcagg
    43621 aggcattgcc cactccacat cgaagctctc ctcaaaggcc gaaatcgcat cgactatatc
    43681 atcataggat atccagtgtg tgctagttac gagatatgga ggagggtcca ttgcactcat
    43741 accatactcg tcagctcgtt ctctgatttc tgttccagga agcagcgaga gtggatataa
    43801 ctctgcttct tgaccgagcc cctgcattcc aagaaaatcg aaggtttcaa tgacctgctc
    43861 atagccatcg tatggcaagc cgagtatgag acctgtcttt attatgattt tctgtctctg
    43921 gagaagttcg gccccgcgtt caaaggcctt tctatcgaag cttctgtgaa tagcttccaa
    43981 agccttggaa tttgtgcttt gaagtcccgc ttcgaccgat acaatgcctg cttcttttag
    44041 aagaccggct atctcttcgt caatatcttc gagtctgagc tcggtatgaa tagacgtatg
    44101 tgcatgattt acaaatgcga tatctcgaag ctttcctgca aaatcaggcc ttatttgaaa
    44161 attggggtcc attatataga tttctggaat acctgcctcc gaggcaaggc gaattacttc
    44221 caggatctgt tcatgcggaa aatagcgaac ggttttataa ttcttcccat aatagcagta
    44281 tgagcatttt gaattgcatc cccgcatagt ctcgatatga acctgaaagt ttggatcgaa
    44341 aagcagagcg ccattcagat atggatttgg caacttgttg aggtctatga gtttgtctga
    44401 actatagaaa gatgaaattg gcctgtgctc gagaatatct tgcagtatat ccgcaaatgc
    44461 ttcttctcct tcgccgcata ttaggctatt aaaaggcgat ttcgatgtaa ttggcattgg
    44521 tttgacgact tc
//
LOCUS       DAOMZL010000005        35742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_882_length_35742_cov_17.379606, whole genome shotgun sequence.
ACCESSION   DAOMZL010000005 DAOMZL010000000
VERSION     DAOMZL010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 35742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35742
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_882_length_35742_cov_17.379606"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLQ30_01030"
     CDS             <1..350
                     /locus_tag="PLQ30_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HPW01058.1"
                     /translation="GRALVLDEQYMDAVTGLSGSGPAYVYLIIEALTDGGVKMGLPRE
                     VSIELAAQTVLGAAKTVLVTGEHPAKLKDQVTTPAGSTIDGLMELEDGGLRVTLIKAV
                     VKATERANKLLQK"
     gene            complement(472..1620)
                     /locus_tag="PLQ30_01035"
     CDS             complement(472..1620)
                     /locus_tag="PLQ30_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01059.1"
                     /translation="MLIERLAKMYVQWGAPKQSIESDRRRNRQIQAVREFLEIIQKTG
                     ENRKELFEVSQQSKRKATKLSYLLLASDTEPAAVRDSFLRSGLLFKGQQNNKPELLMW
                     LAGTEACARGIHPYIVFLIMSAYSGLEAANSEFLWLRERSKLQNAKLTEYIVPGDLTD
                     NIEEALSEPLRFKRTIRIAGMPLAASAFAGCSLYYIEQILALVGPIGSFILTEMIAAA
                     RKRLISDEICTAQQAFLDLYSRQESESSALDKGLMTGMEAFEESSQPDPDLIRTTTDI
                     IMSAEAKILKATLSSMSDNEIASLLRCIEPIAHERLLSLISQGRQRRILTAIQHTDNV
                     TGEKMLRDAQLFAQKLLSSYAPRNLRPGETLKIPELLRNLISSLLGRE"
     gene            complement(1610..2026)
                     /locus_tag="PLQ30_01040"
     CDS             complement(1610..2026)
                     /locus_tag="PLQ30_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01060.1"
                     /translation="MIYWNADLAAKIACSSEDMKILPSYIDYLVDSAKKIAQGVMLPD
                     SEQLSSEKDDFFRYGLLLVSEGLSGEILEEILAVLLYVSKVEGIEFLKQCVAAEAILS
                     IANGEDEEIMIRKLLPYCGIDAALDTVAQRKSEHAD"
     gene            2378..3142
                     /locus_tag="PLQ30_01045"
     CDS             2378..3142
                     /locus_tag="PLQ30_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPW01061.1"
                     /translation="MILAEFDNWARSFLEIDQLRQIDDSLNGIQIACSPSKPIQKMAV
                     AVDACAESIKRAHNVGADLLFVHHGLFWGKPEPIIGSLRERLKILIDSDMALYACHLP
                     LDKHSEVGNNARLADLLELKDRKPFGIYHGISIGWSGVLPKPAKLDKIIAKILPDGSA
                     AKSVIAAGPKEINTVAIVSGGAPFESLEAIREGIDLYITGEPSHSIYHYLIEGGINFI
                     AAGHYATEIWGVKAIAEKAHAELGLDTIFLDIPTGL"
     gene            3144..3680
                     /gene="lspA"
                     /locus_tag="PLQ30_01050"
     CDS             3144..3680
                     /gene="lspA"
                     /locus_tag="PLQ30_01050"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPW01062.1"
                     /translation="MKKNVPLVPILFSGIIIIVDQITKALVVKYIPEGRVFARILGDF
                     VWIVHTRNRGAAFSIGALSPQPLRFLFFICLPIVVLIALLFYYFKSERLSPILRYSIG
                     LIVGGGIGNLIDRILRPQGVVDFISVNMYGFLGMERFATFNIADSAITIGEILLILGF
                     LILEFHKPKAQSSQSEKG"
     gene            3689..3964
                     /locus_tag="PLQ30_01055"
     CDS             3689..3964
                     /locus_tag="PLQ30_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01063.1"
                     /translation="MNKKQANKGKVVLFIVGATVANILLMAICFVLFMLLYSVAFSKF
                     LPQEALIWAIGIAFLLSLLVSSLIYRRLLKLLRERYHLDDYLGLKAK"
     gene            4013..5371
                     /locus_tag="PLQ30_01060"
     CDS             4013..5371
                     /locus_tag="PLQ30_01060"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HPW01064.1"
                     /translation="MRERLRDLGISVPEIYLPREGIDYRKWAVIACDQYSSEREYWED
                     VAREVNDAPSTLRLIFPECFLEDSDIDQRILAVHRTMHEYLEQGLITSRGEAFVLVER
                     TTCLQEKPRLGLIVAIDLEQYQYSEDLKTLIRPTEATIKERLPPRMRIRRGAPLELPH
                     IMLLVDDPKKTLIEPLYERRYYLEKAYDFDLMQNSGHIRGWFVREDREFDRIAQALEN
                     LSDPCTFRARYSHDNGADDVLLFAVGDGNHSLATAKAIWEEIKNAYRDKPDAQRILSE
                     HPARYALVELVNLHDEGLPFHPIHRVLFNIDASDFLRMLSAVGAEIQYRSDARQAFAE
                     VDSEQGSHIFAFVTENKSGTIRFDKPKTLLAVSTIQEYIDEYRKVHPGFGIDFIHGGT
                     SIETIGRKNGNMGIYLPPIKKESLFSIVVHEGLMPRKTFSLGEASEKRFYIESRIISP
                     QQ"
     gene            5490..6362
                     /locus_tag="PLQ30_01065"
     CDS             5490..6362
                     /locus_tag="PLQ30_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HPW01065.1"
                     /translation="MLILLADAFTPDLPERLAEFGEVTSDMSRVAEAEVLIVRSRTKV
                     DGAMLDRAARCRYIVRGGVGLDTIDAEYARTKGIIVDNTPEASSIAVAELAFAFMIAM
                     PNHLVRADISMKEGKWLKKELERSELYGKTLGLVGNGRIGRELAHRAKAFGMSVIAFD
                     KYVMSSDIVRIVCFDELLALSDYISLHIPLIDETRGMIDASIISRMKNGVYLINTCRG
                     QVVVEKDIAEALKCGKIAGYATDVYSKEPPEGSPLFGAPNVLMAPHIGASTEENLLRL
                     ADCIVARLKKYVGR"
     gene            6379..7476
                     /gene="serC"
                     /locus_tag="PLQ30_01070"
     CDS             6379..7476
                     /gene="serC"
                     /locus_tag="PLQ30_01070"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HPW01066.1"
                     /translation="MKRVINFNAGPAALPMEVLRKAQEEMLDWQSTGMSVMEVSHRSS
                     EYEAMHNESQELFRKLAGMGPEWKILFMTGGASTQFFMVPMNYLFGKRKATYVVTGHW
                     SKAAKREAQHFGSVDVISTENKDGSFTRIPKQEELKIDSSSTYVHMTSNNTIYGSQWQ
                     YWPELEGAPLVCDMSSDIFSRPFPVDKLALIYAGAQKNLGPSGVTVVAIREDFLDIAR
                     EGSKLPTMLSYRTFSENNSLYNTPPCFSIYILNLMLKWLLYRIGGLQKMQEINEEKAR
                     ILYEAIDLSDNFYRGPVEKGSRSQMNVVFRLKTPEMEDTFIEKARSAGIVGVRGHRST
                     GGIRFSIYNANLVDNVRKAAEFMEEFQRINR"
     gene            7580..10366
                     /locus_tag="PLQ30_01075"
     CDS             7580..10366
                     /locus_tag="PLQ30_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01067.1"
                     /translation="MAKSRLHRLYNKFISSLSFSAELRKMRRELNVKIDQPESITAPP
                     PFHPQAANRWFKRRRISIAESYLMVVRDLDSRNSSRRLTALKNLADVAFRSSSIDYPL
                     NTARVQSALVKEVVKHRSNKRRQLELLYDFSMSTQGQHQVIRKLCDELNIIELPENGM
                     KIGELGYAWDDHVHDVATSGRKNPTQLVLDAFIKGISSLTVAYGLVSDIDLMEEVLEA
                     GNILGLKVNIGLEFSVLVEGARYHFIAELPHFSSKEELRSFFSDHALDMDKFFKGLEI
                     NRESRLDAVRRLLENFNQNTLPKINEGFENKPEYCLAPLSLEVLLATIPNVNITPLHL
                     AEFMYVRYRPILQKRVWYYKVLREKARYIAQNPSGLQQDQTNGELQREEIEASYSELK
                     KELNRLSPDLVLSAYFDSPHIISYQSAFDDLESISQMLKRAGCSIRFVQPLEHGLELA
                     KQALIKWSRCIDAVEIYNIQDCIGRNPESIEDFARFVNELNKSAAAEGRSFLKPICGS
                     DATGRNPKIPGMGFIFEDQIIGNLRKRYIKRHMRLPSLISAMIRSSESPVDEASLQNA
                     AVPSIVCMGKVSGSWNRAITGDEERINPLRAWRYFNPTFKNAIRAIIGFTVATAYIGP
                     AYALLWLGITGFRNSIADLISYRGPKLSQWKLKSINFDNVGQSLFWTGFSVPLMGYAK
                     STFDILWPLAPETFLFNLVKFFVISIVNGFYLAAHNTLRGFDTNVVRANLFRSILAWP
                     LATIFAPIGNSLSIPSIVQTKIWSDVVAGFIEGGNKYRKVLQLRQKTLEELIPTVINA
                     SGSAQYIATMDVLYLFSQEPRTRTTIKAILSPYAFFTRKLKENSSLRLNMLLEFNDKM
                     SKESLWTDLVDYIVANYEEDMADDLVDLVVDGLPELLGDLSGLIEKYGKDPSLLAKLA
                     AKASGTIRGSGQAK"
     gene            complement(10588..11694)
                     /locus_tag="PLQ30_01080"
     CDS             complement(10588..11694)
                     /locus_tag="PLQ30_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPW01068.1"
                     /translation="MDNEVTWWKFDEVDAFMRAGFEAVGVAPEVATVCSDVLISADKR
                     GVDSHGVGRYKPIYLDRIWAGILDPRTTFDVIRETPTTAVIDGHNGMGLYIAKRSMEL
                     AIEKADKFGIGMTVCRNSTHYGAAFYYARQAVEHGMIGMTTTNARPSIAPTWGVEPML
                     GTNPMTWGMPSDEDFPFMLDCATSVTQRGKIELYDRLGKDLPDGWVIGQDGKYRHDTH
                     QVLIDLGQDKAALTPLGGLGEDFGGYKGYGYAMVVELLSSALSQTNFMKALLGVDSNG
                     KPKPIELGHSFIAINISAFCDLEDFKHHVGEVSRQLRASRKAPGAQRIWTPGEKEHET
                     WLYRKDKGVPFNPPLKKAFKEVKERCKLDIELPF"
     gene            11943..13949
                     /locus_tag="PLQ30_01085"
     CDS             11943..13949
                     /locus_tag="PLQ30_01085"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPW01069.1"
                     /translation="MSAHPYTITLDFENLPAELVFEPGVRRAPKRDAELTPSQERIAL
                     KNALRYIPARWHERLAPEFLAELRQRGRIYGYRFRPQGRIIGLPIDNYSGRCIEGRAL
                     RVMIDNNLDFDVALYPYELVTYGETGQVCQNWMQYRLICKYLEVLDENSTLVVESGHP
                     VGIFASHPFAPRVIITNGLMVGMYDNLKDFNIAAAMGVANYGQMTAGGWMYIGPQGIV
                     HGTYNTILNAGRLKLGIPQDKDLSGRLFVSSGLGGMSGAQGKAAMIANAASIIAEVDY
                     SRIETRLKQGWVSCETDSCEEAVHLAHAALDKGEPIAVAYHGNIVDLLEYIDSHDIHV
                     DLLSDQTSCHVPYDGGYCPVGISFEERTRLLAEDRHYFRALVDASLRRHFEVISRLVK
                     NGTYFFDYGNSFLKAVFDAGVKEISRNGIDEKDGFILPSYVEDIMGPELFDYGYGPFR
                     WVCLSGKKEDLHKTDLAAMSCIDPDRRGQDRDNYIWIRDAEKNKLVVGTQARILFQDA
                     FGRMNIALKFNKMVRNGEIGPVMIGRDHHDTGGADSPFRETSNIKDGSNVMADMATQC
                     FAGNAARGMSLVTLHNGGGVGIGKAINGGFGLVLDGSEKVDQVIRMALPWDAMGGVAR
                     RSWARNVHAMEVADQFNCEYGEYGTITMPHLVDERLLDRFLGAC"
     gene            complement(13891..16449)
                     /locus_tag="PLQ30_01090"
     CDS             complement(13891..16449)
                     /locus_tag="PLQ30_01090"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HPW01070.1"
                     /translation="MANDIRILENKRVGEDFIPSRYLPPGENEYYLRDEQLRTLSKEP
                     ASFRPLSATELEVLIRNNNTCRDWSLFLVRDPFYPELLRNNIFFGLIRISRLEPVTLE
                     HHDFSTATGIYNSLVIACDIGDNCAISDCAYLSHYIIDDHCILINNAELQVSNHAKFG
                     NGIIMESEDESVRITLDVMNENGGRWIYPFNGMLPADAWLWAKFRDRDTLMERFAWMT
                     NTKFDRRLGRYGVIGRDSVLKHNRIIKDTYIGESAYIKGSNKLKNLTINSKSQAPTQI
                     GEGVELVNGILGYGCRVFYGCKAVRFVMCDNSALKYGARLIHSVLGENSTVSCCEILN
                     NLIFPAHEQHHNTSFLIASLIKGQSNLAAGATIGSNHNSRAPDGEIDAGRGFWPGLCT
                     SVKHSSRFASFCLLSKSDYKHELDIRFPFCLVDNDETEGRLVLAPAWWWHSNTYALMR
                     NEKKFKERDKRIDKSIRFIYSPFAPDTMTEILAGLAILECATGKAAIESLVDNKENRR
                     LFGDKILELVSKLRSQLSQESKAENEEKKKNKSAQRPYMQNLDIHNTNISDIHNLGAL
                     ILAKQNDPLPFEVHVEGFENSKRQQVILKPCTARTSYREMLLWFSVTTLLPVLLTELD
                     KMDKLAGSDEILSGLLRCLERENKEFEKAENSQQYKALLREPPEIEWDNMGGFLVSHS
                     KLEDLIDRIETGCIRSWDELHREYHRLSLEYEEDAQAFAWAVLGYLSLDPEKASAINV
                     AIAKGDTSNLGEQWPKPSFSELISSLEAAKELSCAIAQRVYATRAKDWTNPFRKTTFR
                     NENEQLAVHGRIEDNSAIKDVSREMESIGVEIDRLISRLLKSDPKASHPQDGAL"
     gene            16569..17153
                     /locus_tag="PLQ30_01095"
     CDS             16569..17153
                     /locus_tag="PLQ30_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01071.1"
                     /translation="MDFVDSARVDTLVTESEKKVFEIFESMVKSTGQERKKSALALAN
                     LLGNPGEFSFYIDCTEDQRLIRVFHLLRVFRENMTLLINKTWADGSQNTTQELLLGEL
                     ARFIEEYRDFRIVSAFRTFVGIARQIPSLLFGSLGKANDFLEYAFRIDPKFGLFFWYI
                     GEIELQLRNIENLPEHRELFDLEVLIGAFVLSCF"
     gene            complement(17150..17815)
                     /locus_tag="PLQ30_01100"
     CDS             complement(17150..17815)
                     /locus_tag="PLQ30_01100"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HPW01072.1"
                     /translation="MLFLQISHIPAMAIGFVSAMLPLQTSASSCGYAVAAGLINIMRS
                     SAYLAGFEDQELERETYANLYIPAFENDQALAQTYRKASPISLADIKAILARQGIDSM
                     AFKFSPGVLHRLVESLDTPLILHIRGRFSHFVLIIDAKLDAEEDAESAGILLFDPSCG
                     LALLSENELKSLVSGYCLLPIRYACKQGEKPMDIKDLKPSLDSLEAFLWNVFRTLCSK
                     EEN"
     gene            complement(17902..19929)
                     /gene="fusA"
                     /locus_tag="PLQ30_01105"
     CDS             complement(17902..19929)
                     /gene="fusA"
                     /locus_tag="PLQ30_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPW01073.1"
                     /translation="MDLGKIRNIGIMAHIDAGKTTTTERMLYYSGRTYKIGEVDDGEA
                     TMDWMDQEQERGITIQSAATTTFWRDHQINIIDTPGHVDFTAEVERSLRVLDGAIAVF
                     CAVGGVEPQSETVWHQADRYRVPRIAYINKMDRVGANFEAVLQDMKSKLGAHPVPLNI
                     PIGAESSFEGVIDLLEMQEIYWSEDGSVMTKMPIAPERQEIAYHWRENLLDAVSSKSD
                     LITKLYLEAVAIPKDTLIAEIRKACIAQHFVPVFAGASRRNMGVQSLLDAIVDYLPAP
                     DEVGSIKAYHAKREQEIEIPCQISGYPLGLVFKVQYDREAGALCYVRMYSGTMHNSSV
                     VYNNSKKKRERITRLLRMHANKSEPIESVSAGDIAVFVGLKLAQTGDTLGTEGYPVLL
                     EKMHFPEPVISVAIEPRTLSDRDKLKEALDILAREDPTFAVHEDEETGQIIIRGMGEL
                     HLDVIVTRVIREFKVQAKIGNPQVTYRESISTKYRHSEKFHKIIAGKENTAGLTIEVE
                     PLPRGTGNQYKKLVRAPAVPQEIFEAIERGIMSAFSSGIQYGYPAVDIGVSLIDIEYN
                     ELTSTVFAFEACANMAYDAACRAANPILLEPIMKVDILTPKEFVGEVMSLVSQRGGFI
                     HGTETKIVAEIIHAEAPLATMFGFTTSLRSVSQGRASFSMEFSHFEPKREL"
     gene            20193..22250
                     /locus_tag="PLQ30_01110"
     CDS             20193..22250
                     /locus_tag="PLQ30_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HPW01074.1"
                     /translation="MKKVELLAPAGSPEALDAAIGEGADAVYLGLRSFNARMRTTNFA
                     FNQFEAAVYACHNNSRRVYVTVNTVFEERESDRIYQLLEYLASVGPDGIIVQDAGVAK
                     IAHDHFPSLPLHASTQMNIASVAGCNQLSRAGFKRAVLARELSLEEIRQIRQYTSLEL
                     EVFVHGALCISASGLCLFSSYLGGKSANRGQCTQACRRLYESETGKGYYFSPDDLELI
                     EYIPDLIMAGVDAFKIEGRMKSAEYVGAVVSAYRLMLDNYEKDGEKAMLKAKAILQSD
                     FARRKTSFFISGKPEDYIHPEQSGGTGIALGRIRDARTIDGKRWALMNSREGLAERDS
                     VRIHRKDDSGRITTKILAIRYEVNGMLLLLDGDWRQNDELYLIQTASMTRSHKPVLPK
                     NLNRFHKFPSNHTAPRPAISRLDGRKLEISFEPGNYILVGKVAHLHAALTFRPKKAMI
                     LFNKLNAETMRLEEKSLPFKRDRLILWLDPYLAESDSTWLSAELDYWLEKGVSLVILN
                     NQAHFSMLRGKKVISIAGPWLYTFNKWALAYYIEQGVQAFIPPYEISRQDLYRLTEYL
                     PAQFFAPIVFAYPDLFRIRADLSRKYDNAKFMDREDNSFRLIGKRDYSVVVPDRPFSL
                     TDLVPNLKKQGFRRFIVDLSNAEPSKGLYRDIARAMEQGSPLPNTTRFNWKDGFWSEE
                     RMY"
     gene            22418..23200
                     /locus_tag="PLQ30_01115"
     CDS             22418..23200
                     /locus_tag="PLQ30_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPW01075.1"
                     /translation="MSESAGKAFDLLFYLAKAGETVSLARLSRETGMNKATALRYLNV
                     LESKGVVERSKLGWTLGLSLFELGSKVPVRQLVVDKVRPIIERLARETGESVNLAYLA
                     DETAIFLDRAEANRSLRMRSMPGDRLPLYCTGVGKAILSQLSEERMAAILGPYPLEKY
                     TEFTCTDPDDIIKEVRRARELGYGIDSEEFEVGLTCYAMPLRIPSSDFVGAISISGPT
                     ARMKNPEIRDRFIYLLEQAIKDAIELLSPHYHLDIVSKKAKN"
     gene            complement(23286..23702)
                     /locus_tag="PLQ30_01120"
     CDS             complement(23286..23702)
                     /locus_tag="PLQ30_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPW01076.1"
                     /translation="MNHLNSILVEGNLVRDPNFRSTPSGNQVCDFTVATNRNYKIADQ
                     KYENEVSYFDIEAWARLGSACAQNLKKGRGVRVVGRLKQDRWTDTEGKSHSRVKIVAE
                     HVEFKPMSKAAQEGAPKSETPFIEDSKRDAIETPVF"
     gene            23829..25226
                     /gene="purB"
                     /locus_tag="PLQ30_01125"
     CDS             23829..25226
                     /gene="purB"
                     /locus_tag="PLQ30_01125"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPW01077.1"
                     /translation="MENFDRFQSPFTWRYGSEKMREIWSERNKRKLWRHIWIALARAE
                     SRYGLVTPSQIAELESHADDIDFEEANRIEDSIHHDLMAELRVFASQCLEAGGVLHLG
                     ATSMDIEDNADVLRIRDSLSLVKEDFTKLLLALAGKIEDYASLPAMAFTHLQPAEPTT
                     IGYRYACYAQDLAEAYERLSCAIADIRGKGFKGAVGTSASYAELIGLENLDDFERVLS
                     EDLGIEFFDVSTQTYARVQDYRIISLLAEIAAILHKMAFDFRILQMPMLGELCEPFGE
                     HQIGSSAMPFKRNPIECERIESLARLFPSFANIAWDNAALSILERTLDDSANRRIILP
                     EAFLAIDELFSTMRHIIDGQIIDNEAIRCTLDAYAPFSATERVLMALGKRGADRQIAH
                     ERLRNMAMLAWQAVREGNSNPLKSLIESDEFIASFLSSDTIEKLFDVDIYIGAAEKRA
                     LAMAGRIRRLAMDEH"
     gene            25275..25928
                     /locus_tag="PLQ30_01130"
     CDS             25275..25928
                     /locus_tag="PLQ30_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPW01078.1"
                     /translation="MLDFISKGGPILWVIMGLSLVALAIVIERLLYLKRISIDEEKLF
                     LRIKTSLMEGHFSEALAICDQNISPFSKLLKVGIENREKPEYLQRELLKDAASLESVS
                     LERGLTTLGTISNISTLLGLLGTVTGTMRAFGVLGKFGAVSDPAALASGVAEALITTV
                     GGLVVAIPVIMIYNYFVSRINLIMTRLETQVNNLVSFISSYGGKEPERAEEKPSKEA"
     gene            25928..26344
                     /locus_tag="PLQ30_01135"
     CDS             25928..26344
                     /locus_tag="PLQ30_01135"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPW01079.1"
                     /translation="MARNHKLTPKINFDLTPLIDIILQLVIFFMITTTFRTAPGINLQ
                     LPSSKTAQTISTPELRVVVVSSDEIYVDKTKTNIGSLQSVLSKRVSGSDAKQIRAVLE
                     GRAGAEYQLIISVLDALRMNGVENVGLITRKEKVVR"
     gene            26341..27369
                     /locus_tag="PLQ30_01140"
     CDS             26341..27369
                     /locus_tag="PLQ30_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPW01080.1"
                     /translation="MSEALTYEEREQRKRTIHSYIIAIAIYALLLGVGILFDILRPES
                     IDFSNKSLIVNIQGPVTNDIGKGSPIEKEESLVAEKPAPPPVQVPKPQADTSAKPKPA
                     EPLVPSPSVEAEPTPTPIVPETAAVQEPEIPWIPGERGPGSRVSSSESSVLAPGEGQV
                     PWGTGEAVRITKAEKGNTVETTLGGSSDSVGQSLYVPIYLSMPLPEIVPAELFNAIPP
                     EIVPPNTLIASAESRKRAFLNYYSWNGKEYRLTNPVPLEIRDKLWEMLEDAGYNVEKA
                     DYKAGRLLNPVVIGFSITKDRQLRGVEILQSSGDPEIDAAVLYGFKRASFWNKSGDTI
                     QGRFVYRF"
     gene            27448..30624
                     /locus_tag="PLQ30_01145"
     CDS             27448..30624
                     /locus_tag="PLQ30_01145"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW01081.1"
                     /translation="MIINLSSLIAAFSFCIYVFFIIFGISSRKERVSISFLIYMVMMA
                     LWSLGAFMAYSNIGFISLSDAIKIKLVGLLGAPFAIFATLLYFSKSQKKLFRSFLYIG
                     YGLYAYLLYLNFSGSIVENIWFEGLDYHYTLGQDALIVYSLAYVYLLLAILLLLKEQK
                     VNKNKFQKRALRLMLVGVVVLMVGVLAYLYEPVGRYPVDMIAAMINSSIIFFTIYKYK
                     LIHYSSTVFNIILGSLVAAFSSIIYMLFFIFLFKLDLYVPNSTLILLALCLGICSALI
                     FGPLRTAMQTLLEHISGGRSFLYYRELRNFSTKLTSIVKLEELSKLIVDNVIPALKLE
                     WAFVLLNEYRSQNFRVIASHGLDFIQEQGNQNPSNQVLVPKSSPFVQAYQNRASQEEQ
                     VYSQWNIGIMLIKGQKHQTVLASLVLPLKFKERLNGFIILGPRLEKDYYNKYELDVLK
                     FLSDQASVAMENAITYERLRQHQKRLQDANKQLINQRKFEAFFDGIATPISIQDINYN
                     IVTANFAAQRYFEKTQKELIGSKCYKAYFNRDRPCAECLAQDCLHTKLPFNGERQDNR
                     TQLTFSLNFYPIPTQEASLGAFIELFQDISKQKSEQEDTISEEKLAAIGSLVSTIAHE
                     INNPIGGILGTTDLILEEVTEGSTIRGYTEDIARYSKSALDFINSLMVYSKKTHTSLE
                     MLNIINVLENSLKMAMRSIDFGSIIVRKSYDSVKEILANSTELQQVFLSLIINAVQAM
                     RSDGVLTLSCRQEGDDVLVLIQDTGIGMRNQKFDRIVNDFLTVEEASSVSGMGLSFAN
                     HIVTKYGGRILLSSQPGKGTTFTVILPSAIQDKDRIWFINASEPNQFDDSYYIQRKVL
                     VGEKGYKEETIHRKCDETAFHILAYKGLQPIGTVSIHLSEATGHIPIEENFEIGPYLD
                     GAPYAEIDRLAVTKEERQGFTSFEIMVLSYLYVRGRGAKQIFIDTFADETGLIQMFKK
                     LGFEIIGGYSNPLPCTVLMMNHASTYEQETTKNRLFIKKTLSRLVPKIDFSADEMSYV
                     MKAIDEISAISPGKDSVDTKEISSYNLL"
     gene            30626..31687
                     /locus_tag="PLQ30_01150"
     CDS             30626..31687
                     /locus_tag="PLQ30_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HPW01082.1"
                     /translation="MKPEDLAEGLNTILAHSPVFPDKCLIIDVVANPKAGGFSRIHHS
                     KKRMRELKEMVSRSLELPLRKAPLSLKMHLTERCGHAATIVQRILEHSPSNGKDSYHI
                     IITAGGDGTSLETAEQLTLLPESEKDRFGLVRLPFGTGNDGSEGRDLFVALERFLNPA
                     ILERKPALKITPSYEGGALPRYSFNIASIGLDAYVAEMTNRLKRHFPGDSYKFWVDIG
                     TMFYDRVYKVVNMGLRAWNRGRLVLDSELPRLLVAMGASGNRYYGSHKSILPNEHNCI
                     AISQTSLFRKLILKGSIENGKHEHIRELTHFTADRLQIDYHESIPLQCDGETTLLAKC
                     DFPLEMERLQNAYNVLVSV"
     gene            complement(31663..32232)
                     /gene="thpR"
                     /locus_tag="PLQ30_01155"
     CDS             complement(31663..32232)
                     /gene="thpR"
                     /locus_tag="PLQ30_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPW01083.1"
                     /translation="MRLFVALPFPAAVQNQIGTYCGSIETAFRPARPSWVPPQNLHLT
                     LHFFGELEVEKAKELENLLSDAANLCPPLRITTEGLSVLPAMNRPRVLYLAARIEPSE
                     PLTILIDRIREIAAQLGAQTDARPWKAHLTLARLKLPYAPNLSSLPKPPMLSLYIDSF
                     ELLQSTLSPVGAIYSKIRRFALYTDTSTL"
     gene            complement(32240..32971)
                     /locus_tag="PLQ30_01160"
     CDS             complement(32240..32971)
                     /locus_tag="PLQ30_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HPW01084.1"
                     /translation="MKNARATLSKLCEIALRRYDMIQPGDRILIAVSGGKDSCCLAWD
                     LSKKRQWLETPFEIAACHIVTDLEDFGLSQRRDDSWLALYMTKWGIPYTRLEIPVVGR
                     LKPGRSMNCYWCTTQRRSELMKFAKEHGFNKLALGHHMDDILETLLMNMLRKGEFATM
                     PPVMPYRKYPITVIRPLALCEEKQVLACADELGLSDYTYTCSFNLEGERKKARELLEH
                     LTEGSSQKKRNIFESMSRIRKDYLT"
     gene            complement(33095..33700)
                     /gene="udk"
                     /locus_tag="PLQ30_01165"
                     /pseudo
     CDS             complement(33095..33700)
                     /gene="udk"
                     /locus_tag="PLQ30_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537791.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0046108 - uridine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
     gene            complement(33744..34628)
                     /gene="ispH"
                     /locus_tag="PLQ30_01170"
     CDS             complement(33744..34628)
                     /gene="ispH"
                     /locus_tag="PLQ30_01170"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPW01085.1"
                     /translation="MQIVRARILGMCMGVRRADELAHRAAERAARTGRPVYSYGPLIH
                     NPQAVADLESLGVHVLDPSTIESELDELPNLRDGIVIIRAHGASLAAISRLHVLGARI
                     IDATCPRVIKSQGLARHYERLGWQVVLVGDSRHGEIAGILGHTLNALVVDSPETAPAL
                     ARSLRDKPCALIAQTTIRQEDYDTVIDIFQSYVRKMIAEKTICPATRERQQALAELST
                     QVDALLVVGGKNSENTKRLYRSAIGFGLPSWHIETADELKPEMVSYDRIGITAGASTP
                     DFIIDEIQEMLIRMAQNG"
     gene            34742..35686
                     /gene="arcC"
                     /locus_tag="PLQ30_01175"
     CDS             34742..35686
                     /gene="arcC"
                     /locus_tag="PLQ30_01175"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPW01086.1"
                     /translation="MAMRKTIVVALGGNAIIEEGTEGTIEQQFENTRKSMKAIVGMIA
                     EGHKVVLTHGNGPQAGVHLIRNEAASEQVPPSPLNVIVADTQGSMGYMIAQSLSNALR
                     TVGIDKDVVTVITQVVVDPNDPSMLNPTKFVGPFYKFEEVEKLRARGWVIKEDPMRGY
                     RRVVPSPKPLDVIEKETIKDLIDDGKIVIAVGGGGIPVKRNPDGTLSGVDAVIDKDRA
                     SSLLASLINADELIILTGVDKVAINFRKPDQRVFDHLSLRECEQYMAEGHFPKGSMGP
                     KIEAACDFIRRGGAKVIITSMENATAAVDGKAGTVISA"
BASE COUNT         9412 a         8062 c         8568 g         9700 t
ORIGIN      
        1 ttggccgtgc tcttgtctta gatgagcagt acatggatgc cgttacaggt ctttccggtt
       61 ctgggcctgc ctatgtttac ctgataattg aagcactgac cgatggtggg gtaaagatgg
      121 ggctgcctcg cgaggtttcc atcgagctgg cagcacagac tgtgcttggt gcggctaaga
      181 ccgtgcttgt tacaggggaa catccggcta aactcaaaga tcaggttaca actcctgctg
      241 ggtctactat cgatggcctt atggaactcg aagatggcgg actacgggtg acattgatca
      301 aggccgtggt aaaggcaact gagcgagcta ataagctttt gcagaagtga ataccagctc
      361 aatggttcaa gattgttttc aggctttgcc attggttttg tctctctaga gctattttgc
      421 cttgattatt agcgctttgc tctcggcacc gcttagaggg aagtctatct tttattctct
      481 tccaagcaat gaagaaatga gatttctcaa aagctccgga attttgaggg tctcgccagg
      541 ccgaagattt cttggagcat atgatgaaag caatttctgc gcaaaaagct gggcatcgcg
      601 gagcattttt tctccagtca cattatccgt atgctgaatg gctgtgagta tacgcctctg
      661 tcgcccttgc gaaatcaggc ttaagaggcg ttcatgtgca atgggctcga tgcatcgcaa
      721 gagggaagca atttcgttat cgctcatgga agagagagtt gccttgagaa ttttcgcttc
      781 ggcggacata attatatcgg tcgttgtacg gattaggtca ggatctggct gactagattc
      841 ttcaaaggct tccattcctg tcatcaggcc tttatctaaa gccgagcttt cggattcttg
      901 ccgagaatat aaatcgagga aagcttgctg agcggtacat atttcatcgg aaataagtct
      961 tttccgcgct gcagctatca tttctgtgag gatgaatgaa ccaataggcc caacaagcgc
     1021 gaggatctgt tcaatataat agagtgagca tccagcaaag gcggatgcag ccaaaggcat
     1081 acctgcaatt cggatggtac gcttaaacct caaaggctcg cttagcgctt cttcgatgtt
     1141 gtcggtgaga tctccaggca cgatatattc ggtaagctta gcattttgaa gctttgacct
     1201 ctccctgagc cataggaatt ccgaattagc tgcctcaagg ccggaatatg ctgacataat
     1261 aagaaatacg atataaggat gaatgcccct ggcgcatgct tctgtcccgg ccaaccacat
     1321 gagcagctca ggtttattat tttgctgccc tttgaacagg aggccagagc gcaaaaagga
     1381 atctcggact gctgcaggtt cggtgtcgct tgcaaggagg agataagaca gcttggttgc
     1441 ctttctcttg gattgttgtg atacttcgaa cagttccttg cggttttcgc ccgttttttg
     1501 gatgatttcc aggaattcgc ggactgcttg aatctggcga tttcttctcc gatccgactc
     1561 gatagactgt ttaggcgctc cccattggac atacattttg gcgaggcgtt caatcagcat
     1621 gctctgattt cctttgtgct acggtgtcaa gagctgcatc tattccacag tatggcaaga
     1681 gtttgcggat cattatttcc tcatcctcgc catttgctat ggaaagaata gcctcggcgg
     1741 caacgcattg tttcagaaat tcgatacctt cgaccttgct tacgtataga agcacggcga
     1801 gaatctcttc taatatttca cctgatagac cttcgcttac cagcaggagg ccatatcgga
     1861 aaaagtcatc tttttcggaa gataattgct ctgaatcagg caacatgact ccttgcgcaa
     1921 tcttcttggc cgagtctacc aggtaatcga tataagaagg caagatcttc atatcttccg
     1981 aactacatgc tattttggcg gcgagatcgg cattccaata gatcatttta tctccgcata
     2041 ttcctgttta tgccagatta tagtatgaaa tatgggtccg aagaaatggg gctcaaaggc
     2101 acagctatca aaacggatta ggtttcttcc tattgcacaa aagcgcatat tatggtatat
     2161 tttgtgagct attgtgaaat ctaacaatat ttggtaagat tttcataatt tttcatagcc
     2221 aatgtagtct cagaatattg acgcaattta tagaatgtga tatattcaaa ttgaaagcaa
     2281 taagcgagtg tcggtggcga aacgacatta tatagctttt gaacccttta tggtctgcct
     2341 ccttccatga agggttcttt ttttttatac tagttccatg atactagctg aattcgacaa
     2401 ttgggctcga tcttttcttg aaatcgatca gcttcgacag atcgatgatt ccctcaatgg
     2461 cattcagatt gcctgcagtc cttcaaaacc catacaaaag atggcggtcg cggtggatgc
     2521 ctgcgctgaa agcatcaaaa gagcgcacaa tgtgggtgct gatcttttat ttgtccatca
     2581 tggtctgttt tgggggaagc cagaaccgat aataggcagc cttagagaaa ggctcaaaat
     2641 tctaattgat tccgatatgg ctctctatgc atgtcatctt cctctagata aacattccga
     2701 ggtaggcaac aatgcccgtc tcgcggatct gcttgagctt aaggatagaa agccttttgg
     2761 catatatcat ggcattagca ttggatggtc gggtgttcta ccaaagccag ccaaactgga
     2821 taaaatcata gctaagattc ttccggatgg gtcggctgcg aaatccgtaa ttgctgccgg
     2881 gccgaaagag atcaatactg ttgccattgt atcgggtgga gccccattcg agtctctgga
     2941 agccataagg gaaggtatcg atctctatat aacaggtgag ccttcgcatt ctatctacca
     3001 ttatctcata gaaggtggca taaactttat tgccgctggg cactatgcta cagagatctg
     3061 gggtgtcaag gccatagcgg aaaaagctca cgcagagctg ggtctcgata ctattttcct
     3121 cgatataccc accggattgt gagatgaaga aaaatgtgcc tctcgttcct attttgtttt
     3181 ctgggattat aattattgta gatcagatta caaaggcgct cgttgtaaag tatattccag
     3241 aaggaagggt ttttgcacga attctgggag attttgtgtg gatagtgcac actaggaaca
     3301 ggggggcagc cttttccatc ggtgctcttt cgccgcagcc gctcagattt ttgtttttta
     3361 tttgcttgcc tattgttgtg cttatagcat tgcttttcta ttatttcaaa tcggaacgcc
     3421 tttctccgat actcagatat tctataggtc ttatagtggg tggaggaata ggaaatctga
     3481 tcgatcgaat tctacgaccc caaggggttg tggattttat ttcggtcaat atgtatggtt
     3541 ttcttggaat ggagcgattt gcgaccttca atattgccga ttctgcgatt actattggcg
     3601 agatactctt gatacttgga tttctgattc tagagtttca caaaccgaag gcacagtcgt
     3661 cgcagtctga aaagggatga ggatcgatat gaataagaaa caagccaata aaggcaaggt
     3721 tgttctcttt atagtcggag caaccgtggc gaatattctt ctgatggcga tttgttttgt
     3781 gcttttcatg ttgctctatt cggttgcttt ttctaagttt cttccgcaag aagccttgat
     3841 atgggcgatc ggcatagcat ttttgctttc tcttctcgta tcctcgctca tctatcgtcg
     3901 tttgctgaaa ttgttgagag agcgctatca cctggacgat tatcttggcc taaaggcaaa
     3961 atagaaagac accgatatct aatcgaatgt tggatgatta aggcaagcaa aaatgcgcga
     4021 aagactaaga gatttaggta tctcagttcc tgagatatat ctgccaagag aaggcatcga
     4081 ttatcggaaa tgggctgtga tagcatgcga tcaatattct tcagaacgcg aatattggga
     4141 ggatgtggct cgcgaagtaa acgatgctcc ttcaaccctt agactgattt ttcccgaatg
     4201 ctttctcgaa gatagtgaca tcgatcagcg aatcttggct gtacatagga caatgcatga
     4261 gtatttggaa caaggtctaa tcacctcgag gggggaagcc tttgtgctgg tggaacggac
     4321 gacatgcttg caagaaaagc ccagacttgg acttattgtg gcaatcgatc tggaacaata
     4381 tcagtatagt gaggatttaa aaacgctgat ccggcccaca gaagccacaa ttaaggaacg
     4441 tcttccacct cgtatgcgga ttaggcgcgg tgctccgctg gaactaccac acattatgct
     4501 cttagttgat gatcccaaga agactcttat agagccgctc tatgaacgaa ggtattacct
     4561 cgaaaaagct tatgatttcg atttgatgca gaactcaggc catatccgtg gttggtttgt
     4621 gagagaagac agggagtttg acagaattgc acaggctctc gaaaacctct cggatccttg
     4681 tacattccgt gcgaggtata gtcatgacaa tggtgcggat gatgttttgc tctttgcggt
     4741 tggcgatgga aatcattcgc ttgctacagc aaaagctatt tgggaagaga taaagaatgc
     4801 atacagagat aagcccgacg cccagagaat tctctcggag catccagcac gctatgcgct
     4861 ggtagagctc gtgaatctcc atgatgaggg gctccccttt catcctattc atcgcgtact
     4921 ctttaatatc gatgcttcgg atttccttcg aatgctgagc gccgtcggcg cagagataca
     4981 atatagatcc gatgcaaggc aggcatttgc ggaggtcgat tcggagcaag gttctcacat
     5041 ctttgcattt gtaacggaga ataagtcggg aacgattcga tttgataaac ctaaaacgct
     5101 gctcgcagtc tccaccattc aagagtacat cgatgaatac cgcaaggttc atccagggtt
     5161 tggtatcgat ttcatacatg gcggcacaag catagagact attggccgca aaaatggaaa
     5221 tatgggaatc tatcttccgc ctataaaaaa agagtcgctt ttcagcattg tggttcatga
     5281 gggcttaatg cctcgaaaga cattttcgct gggcgaggct tcggagaagc gcttctatat
     5341 cgaatctagg atcatttcgc cgcaacaata acataccgct ccgaaccttt tcttatacac
     5401 aaggttcgta gcagtagaga aggtctgcat atatattcta ttctagattt ccaaagagtg
     5461 ctattatcct tcgaaaccgg gaggtgcaaa tgctaatact gcttgcagat gcctttacgc
     5521 ctgatttgcc ggagcgactt gcggaatttg gcgaggttac ttcggatatg tcgcgggttg
     5581 ctgaggccga ggtgttgata gttcgctcca ggacaaaggt cgatggagcc atgctcgata
     5641 gggcagctcg ttgtagatac attgtaagag gaggtgttgg gcttgatacc attgatgccg
     5701 aatacgcaag aactaaaggt attatcgtgg ataatactcc cgaagcctcc agcatcgcgg
     5761 ttgccgaact tgcctttgcg tttatgatcg caatgcccaa tcatcttgta cgagccgata
     5821 tatctatgaa agaaggaaaa tggcttaaga aagaacttga aaggagcgaa ctctatggga
     5881 aaacactcgg tctcgtaggc aatgggcgaa ttggtcggga gttggcgcac agggcaaagg
     5941 cttttggtat gtccgtgatt gctttcgata aatatgtaat gagttccgat atagtaagaa
     6001 ttgtctgctt cgatgagctt ttggcgttat cggattatat tagcctacat attccactta
     6061 tagacgagac aagaggcatg atcgatgcgt ccataatctc ccggatgaag aatggagtct
     6121 atcttataaa tacatgcaga ggccaggttg ttgtggagaa agacatcgcc gaagccctca
     6181 aatgcggcaa aattgcaggc tacgcgaccg atgtatattc gaaggaacca cccgaaggct
     6241 ctccactctt tggagcgcct aatgtgctta tggcgcctca tattggagca tctaccgagg
     6301 aaaatcttct gagattagcg gattgcatag ttgcaagact caagaaatat gtgggtagat
     6361 gagaaaggaa ttaatttcat gaaacgagtg ataaatttca atgccggacc agctgcgctt
     6421 cctatggaag tattgcggaa ggctcaagag gaaatgctgg attggcagag tacgggcatg
     6481 tcagtcatgg aagtctctca tcgatctagc gaatacgagg cgatgcataa tgaatcacag
     6541 gagctttttc gcaaactcgc aggtatggga cccgagtgga agatcctatt catgaccggt
     6601 ggagcttcga cgcagttctt tatggttccg atgaattacc tctttggcaa acgtaaggca
     6661 acttatgttg ttactggaca ctggagcaag gctgctaaga gggaagcaca gcattttgga
     6721 tctgtcgatg tcatcagtac cgaaaacaag gatggtagct ttacacgaat tccaaagcag
     6781 gaagagctaa aaatagactc ttcctcgact tatgtgcaca tgactagcaa caacacaatc
     6841 tatggttctc agtggcaata ttggccagag ttggagggag cccctcttgt atgcgatatg
     6901 tccagcgata ttttttctcg tccgtttccg gtcgataagc ttgccctcat ctatgcgggc
     6961 gctcagaaga atcttggccc ttcaggtgtc accgttgtcg caatcagaga ggacttcctg
     7021 gatattgcac gggaagggtc caaattgccc acaatgcttt cctatcgcac tttttcggag
     7081 aacaattcgc tatataatac accaccatgc ttctcgatat atatacttaa ccttatgcta
     7141 aaatggctgc tctatagaat cggcggtctc caaaaaatgc aagaaatcaa cgaagagaag
     7201 gctcgtattc tctatgaagc catcgatcta tcggataact tctatcgagg gccggttgaa
     7261 aagggctccc gttcacagat gaatgttgta tttagactca aaacgccgga aatggaagat
     7321 accttcatcg agaaggcaag gtcggctgga atagttggag ttagggggca tcgttcgaca
     7381 ggtggaattc ggttttcaat atataatgcc aatcttgtcg ataatgttag aaaagccgca
     7441 gaattcatgg aggagttcca gcgtatcaac cgctgaatcc tgttagattt ccaattcagt
     7501 aggctggtct aaggattcgg aggccagcct ttttattttt gactgatgtt ttatatgaaa
     7561 ggataggtta tattgaatta tggcgaaatc caggctgcac aggctataca ataagtttat
     7621 ctcttctctc agtttttcgg ccgagctgcg caagatgcgc cgtgaactca acgtaaagat
     7681 agatcagcct gaaagtataa ctgcgcctcc ccctttccat cctcaggcag ctaaccgctg
     7741 gtttaagcgc cgaagaattt ccatagcaga atcttatctg atggtggtta gagacctcga
     7801 ttctcgcaac tccagcaggc gcttgacagc cctaaaaaac ctcgccgatg tcgcctttcg
     7861 ttcatcgagc atcgactatc cattgaatac ggcaagggtc cagtcggctc tggtcaaaga
     7921 agttgtaaaa caccgctcga ataaacgacg tcagttggaa ttactctatg atttttcgat
     7981 gtccacacaa ggacaacacc aggtgatacg taagctctgc gatgagttaa atatcataga
     8041 gctgccagag aatggcatga agattggaga acttggatat gcctgggatg accatgtcca
     8101 tgatgttgca acatccggaa gaaaaaatcc gacccagctc gtactggatg cctttataaa
     8161 gggcatatcg tcgctgaccg tagcttatgg cctcgtttcc gatatcgatc tgatggagga
     8221 agtgctggaa gcaggcaata tccttggcct taaggtaaat attggactcg agttcagcgt
     8281 tctggtggaa ggggctcgct accattttat agccgagctt cctcattttt cgtcgaagga
     8341 ggaattgcgc tcctttttct cggatcatgc attagacatg gataagttct ttaagggact
     8401 cgaaatcaat agagaaagca gactcgatgc ggtacgtcgt ctactagaaa atttcaatca
     8461 aaatactctg ccaaaaataa atgaaggctt cgagaataaa ccagaatact gccttgcccc
     8521 tctttcgctg gaagtattgc ttgcaactat tccaaacgtg aatatcacgc ctcttcatct
     8581 tgcagaattc atgtatgtgc gctataggcc aattctccag aaaagagtgt ggtactataa
     8641 agtccttcga gaaaaggcaa ggtacatagc gcaaaatcct tcaggccttc agcaagatca
     8701 aactaacggt gagttgcaaa gagaagagat cgaagccagt tattccgaac ttaaaaagga
     8761 attgaatagg ctttctcctg atttggtgct ttcggcctat ttcgattccc ctcatataat
     8821 ttcctatcag tccgcttttg acgatcttga atctataagc caaatgctaa aaagagcagg
     8881 atgctccata aggttcgtcc agcctcttga gcatgggctt gaactagcca aacaagccct
     8941 gatcaaatgg agtcgatgca tcgacgcggt ggaaatctat aacatacagg attgtatagg
     9001 aagaaatcca gaatcaatag aagactttgc gcgttttgtc aacgagctca acaagagtgc
     9061 ggcagctgaa ggtcgaagct ttctaaagcc tatctgtggg agtgatgcca cagggagaaa
     9121 tccaaagatc ccgggcatgg gattcatatt cgaggatcag attatcggaa atttgcgcaa
     9181 gcgatacata aaacgacata tgcgtctccc atcgcttatt tctgctatga ttcgatccag
     9241 cgaatctccg gtggacgagg caagtctaca aaatgctgct gtcccttcta ttgtctgcat
     9301 gggcaaggtt tcgggtagct ggaatcgcgc aattacaggc gatgaagaga gaataaaccc
     9361 attgcgcgct tggcgatact tcaacccgac attcaagaat gcgattcgtg cgattattgg
     9421 ctttaccgta gctacagctt atataggccc tgcttatgcc ctgctttggc ttggtatcac
     9481 gggttttcga aatagtatcg cagacctcat ttcctataga ggtcccaaac tcagccagtg
     9541 gaaacttaag agcatcaatt ttgacaatgt cggtcaatcg cttttctgga ctggcttttc
     9601 tgtacccctt atgggatatg caaaaagcac ttttgatata ttgtggcctt tagctcctga
     9661 gacattctta ttcaaccttg taaaattctt tgttatttcg attgtaaatg gcttctacct
     9721 tgcggcgcac aacaccttga ggggcttcga tacgaatgtc gttagagcca atctattcag
     9781 aagtattctg gcctggcctc tggctaccat atttgcgcca atagggaatt ctctatctat
     9841 tccaagcatc gtgcagacca agatatggtc agatgttgtt gccggcttta tagaaggtgg
     9901 aaataaatat cgcaaggtac tacaactgcg ccagaagacc ctggaagaac tgattcccac
     9961 tgttataaat gctagcggca gtgcgcaata cattgcgacg atggatgtcc tttacctctt
    10021 ttctcaagaa ccgcgcacta gaactacgat caaggcgatc ttgagtccct acgctttctt
    10081 tactcgtaag ctaaaagaaa attcctcatt gcgcttaaat atgctcttgg aattcaatga
    10141 taaaatgagc aaggagagtc tgtggacaga tctcgtcgat tacatcgtgg caaattacga
    10201 agaggatatg gcagacgatt tggtagacct tgttgtggat ggcctacctg agcttttggg
    10261 agatctaagt ggcctgatag aaaagtatgg gaaagatcca agccttcttg caaaactggc
    10321 tgcaaaagcc agtggaacaa ttcgaggctc ggggcaggct aaataaaata cctatggctt
    10381 aaagagtccg gaaggcaatt attgctcata agttccatac atataaatca tcgatagact
    10441 ctaaatcata aacaaaccaa taagccacaa attcagagct ataaagtctt gtggtctttt
    10501 tttgtgattt tgactcgcgg ccttatagct ctgagtaatg ccttgcatgc aatgcatgct
    10561 gcatggatcg cttaggaata tcgcaaatca gaagggtagt tctatatcga gtttgcagcg
    10621 ctccttgact tctttgaagg ccttcttaag gggcggattg aagggcacac ccttgtcttt
    10681 tctgtatagc catgtctcat gctctttttc gccgggcgtc catatgcgtt gagctcccgg
    10741 ggctttcctt gacgctctga gttggcggct tacctctcca acatgatgct tgaaatcctc
    10801 caggtcgcag aatgcgctga tgttgatggc aataaagctg tgccctaatt cgatgggctt
    10861 gggcttgccg tttgagtcga cacccagaag agccttcatg aagttggtct gagagagggc
    10921 gcttgaaagc agctccacca ccatggcata gccatagcct ttgtagccgc caaaatcctc
    10981 accgaggccg ccaagaggag ttagagccgc cttgtcctga cctaagtcta tcaagacctg
    11041 gtgagtatcg tgtctgtatt ttccatcctg tcctatcacc catccatccg gaagatcctt
    11101 gccgagtctg tcgtagagct cgattttgcc gcgctgtgtc actgaagtgg cgcagtccaa
    11161 cataaaggga aagtcctcgt ccgaaggcat gccccatgtc atgggattag tgcctagcat
    11221 gggctcgact ccccaggtgg gagcgatgga aggccgtgca ttagtggtgg tcatgccaat
    11281 cataccatgc tcaacggctt ggcgagcata gtagaaagct gcgccgtagt gtgtcgaatt
    11341 tcgacacacc gtcatgccta taccaaattt atccgctttt tctatggcca attccatgga
    11401 cctttttgcg atatacagac ccataccgtt atgaccatcg attactgccg tagtcggagt
    11461 ctcccttata acatcgaagg tcgttcttgg atctagaatt cccgcccaaa tacgatcgag
    11521 atagattggt ttatatctac ccacgccatg agaatcgacg ccgcgcttat cagcggagat
    11581 tagtacgtcc gagcatacag tcgcgacctc gggagccacc ccgacagcct cgaatccagc
    11641 cctcataaag gcgtctactt cgtcgaattt ccaccatgtg acttcgttgt ccattattct
    11701 ttctccttat gaatagattt gtagttcaag gttctgtaga acatgatctt tccaacgata
    11761 tacatcataa gactaaaggc gagatattga caattagcgt tcggaggacc ttgaacaaga
    11821 attgaaatga aaagccgaga cataaaatcg aggaagattc ttcgtttcaa ttctcattat
    11881 tgatgataca attctattga tacaagatat ataaacaaac gatagaaaag gaggtcagta
    11941 taatgagcgc tcatccatat acaataacgc tagattttga gaatcttcca gccgagctgg
    12001 ttttcgagcc aggggtcaga cgagcgccaa agcgcgacgc agaactaaca cctagccagg
    12061 aacggatcgc attaaaaaac gccttgcgct atattccagc tcgctggcat gaaagacttg
    12121 ctccggaatt tctggcggag ctcagacagc gaggtcgcat ctatggatat cgttttcggc
    12181 ctcagggaag gattatcggc cttcccatcg acaactattc cggcaggtgc attgagggtc
    12241 gtgctctccg agtaatgatc gacaataatc tcgacttcga tgtggcactc tatccgtacg
    12301 agctcgtcac ctatggcgag accggacagg tatgccagaa ctggatgcag tatcgactta
    12361 tttgtaagta cctcgaagta ctagacgaaa acagtacgct tgtagtggaa tctggccatc
    12421 cagtgggcat ttttgctagc catccttttg cgccgcgtgt gatcattaca aatgggctca
    12481 tggtgggcat gtacgataat ctcaaggact tcaatatcgc cgcggcaatg ggtgtagcaa
    12541 attatggcca aatgaccgca ggcggctgga tgtatattgg gccacagggt attgtgcatg
    12601 gcacctataa cacgatcctg aatgcaggca gactcaaact gggcatcccg caggataagg
    12661 atctttctgg aagactattt gtgtcttctg ggctaggcgg tatgtctgga gctcagggca
    12721 aggcggctat gatagctaat gcggcctcta tcatcgctga agtggattat tcgcgaatcg
    12781 aaacaagact caaacagggt tgggtttctt gcgaaactga ttcatgcgaa gaagctgtcc
    12841 accttgctca tgccgccctg gacaagggag agccaattgc ggtcgcatat catggcaaca
    12901 ttgtcgatct tcttgaatat atcgatagcc acgatataca tgtggacctg ctttcagatc
    12961 agactagctg ccatgtccca tatgatggcg gttattgccc ggtcggcata agcttcgaag
    13021 agcgtacaag gcttttggct gaggataggc attatttccg ggctttggtc gatgcttctc
    13081 tgcggcggca cttcgaagtc atatcgcgcc ttgtcaaaaa tggcacatat ttcttcgatt
    13141 acggaaattc gttcctcaag gctgttttcg atgctggcgt aaaagaaatc tcaagaaatg
    13201 ggatagatga aaaggatgga ttcattttac cgagttatgt agaagatatc atgggcccag
    13261 agctcttcga ctatggatat gggcccttcc gctgggtatg cctttctggc aaaaaagaag
    13321 acctccacaa aaccgacctc gccgcgatgt cctgcatcga tcctgatcgc cgaggccagg
    13381 atcgcgataa ctatatttgg atacgcgatg cggagaaaaa caagcttgtt gtaggaactc
    13441 aagcacgcat tcttttccag gatgcctttg gccgcatgaa tatcgcgctg aagttcaata
    13501 aaatggtgcg caatggcgaa attggcccgg ttatgatagg tcgtgaccat cacgacaccg
    13561 gcggcgcaga cagccccttc cgcgagacga gtaatatcaa ggacggctcg aatgtcatgg
    13621 cggatatggc aacccaatgt tttgccggca atgcagcaag aggtatgagc ctcgtaaccc
    13681 tacacaatgg cggcggtgta ggcatcggca aagccataaa tggtggattt ggccttgtgc
    13741 tcgatggctc ggagaaggtc gaccaggtta tacgtatggc cttaccatgg gatgcaatgg
    13801 gcggcgttgc ccggcgctct tgggcgcgca atgtccatgc catggaggta gccgatcagt
    13861 tcaattgcga gtatggcgag tatggcacta ttacaatgcc ccatcttgtg gatgagaggc
    13921 ttttggatcg ctttttagga gcctgctaat cagtctgtct atctccaccc ctatggactc
    13981 catttcacgg gagacatcct ttatagcact attatcttct atgcggccat gtactgcgag
    14041 ttgttcattt tcatttcgga aggttgtctt tctgaaagga ttagtccaat cttttgcacg
    14101 ggtagcatag acgcgctggg cgatagcgca agatagttcc tttgcggctt cgaggctaga
    14161 gataagctca gaaaaagagg gctttggcca ctgttctccc aaattcgatg tatctccttt
    14221 agcaatggcc acattgatgg cactggcctt ttcaggatca aggcttaagt agccaagaac
    14281 agcccaggca aaagcttgag catcctcttc atattccaag gagagtctat gatactcacg
    14341 atgaagctca tcccagctac ggatacaccc tgtctctatg cgatcgatta gatcttcaag
    14401 cttggaatgg ctcacaagga aacctcccat gttatcccat tcgatttctg gaggttctct
    14461 aagcaaagcc ttatactgct ggctattctc cgccttctcg aattccttgt tttctcgttc
    14521 tagacatcgc aataatccgc tcaaaatctc atctgatccg gcaagcttat ccatcttgtc
    14581 taattctgtt aaaaggacgg gcaggagcgt tgtcacagaa aaccagagca acatttcacg
    14641 ataggaagtt cttgctgtgc atggctttag aatgacttgt tgtcttttgg aattctcgaa
    14701 gccctctaca tggacttcaa aaggcagcgg gtcattttgt ttggcaagga tgagagcgcc
    14761 caggttgtga atatctgaga tatttgtgtt atgaatatca agattctgca tataaggcct
    14821 ttgtgcggac ttgttcttct tcttttcttc gttttctgcc ttagattctt ggctcaactg
    14881 cgatctgagc ttgctcacaa gttcgaggat tttgtcgccg aatagccttc gattttcctt
    14941 gttgtcgacc agcgattcta tggctgcttt tccggtggcg cattcaagaa tcgcgaggcc
    15001 tgcaagaatt tcggtcattg tgtccggcgc aaaaggagaa tagataaagc ggatgctctt
    15061 atcgattctc ttatcgcgct ccttgaattt cttttcattt ctcatgagcg cataggtatt
    15121 ggaatgccac caccaggcgg gagccaatac aagcctgccc tcggtctcat cgttatccac
    15181 gaggcagaag ggaaaacgaa tatcgagctc gtgcttatag tcactcttcg aaagaaggca
    15241 aaacgaagca aagcgcgatg agtgtttcac ggaagtgcaa agccccggcc aaaaacctcg
    15301 ccctgcatcg atttctccat cgggtgcgcg cgaattatgg tttgagccga tcgtagcgcc
    15361 cgcggcgaga ttgctctggc cttttatgag gcttgctatc aagaaactcg tgttatgatg
    15421 ctgctcatgg gctggaaaaa tgaggttatt gagaatctcg cagcaggata cggtgctgtt
    15481 ctcgccaagg acggagtgaa ttagtcttgc tccatatttc aaggctgagt tatcgcacat
    15541 gacaaatcgc accgccttgc atccatagaa cacgcggcag ccatagccca ggattccgtt
    15601 gacgagctct acaccttcgc caatctgagt cggtgcttgg ctcttggaat tgatggtgag
    15661 gtttttcagc ttgtttgagc cttttatata agcgctttct ccgatatagg tatctttgat
    15721 gatgcgattg tgtttgagca cgctgtcacg gccgataact ccgtatctac ccaacctgcg
    15781 atcgaatttc gtgttggtca tccatgcaaa acgctccata agagtatctc tatcgcgaaa
    15841 tttcgcccag agccaggcat ccgcaggaag cattccattg aatggataga tccagcgacc
    15901 accattctca ttcataacat cgagcgttat tcggaccgac tcatcttcgc tttccattat
    15961 aatgccattg ccaaatttgg cgtgattaga gacttgcaat tccgcattgt tgatgagaat
    16021 acagtgatca tcgatgatat agtgtgaaag ataggcacaa tccgaaatgg cacaattgtc
    16081 cccaatatcg caggcaataa ccagcgagtt gtatattccg gttgcagtgg agaagtcatg
    16141 gtgttccaag gtgacaggct caaggcggga aatccgaata agcccgaaga atatgttatt
    16201 cctcaaaagc tcgggataaa atggatcccg cacaagaaac aaagaccagt ccctgcatgt
    16261 gttattgttt cttataagca cttctaattc ggtggctgaa agtggtctga aagatgcggg
    16321 ctccttacta agagttctaa gctgttcatc gcgtagatag tattcatttt caccgggagg
    16381 cagatatcga gatggaatga agtcttctcc cactcttttg ttttcgagaa tcctaatatc
    16441 atttgccatg tttcaagtat gaaatactct ttgttcttgt gcaactggac gaatagacca
    16501 tcgagcgaaa agttagagct tctcttatcc cccttgacca tttcatgcct tttcgtcatg
    16561 gtattgccat ggattttgtc gattccgcca gagtggacac tctcgtaacc gaatccgaga
    16621 aaaaggtctt tgagattttc gaatcgatgg taaaatccac agggcaagaa cgaaagaaat
    16681 ccgcgctggc tctggcaaac cttttaggaa acccagggga attcagtttc tacatcgatt
    16741 gtacagaaga tcaacgccta atacgtgtgt ttcatctttt gcgcgtattt cgagaaaaca
    16801 tgacacttct aataaataag acctgggcag atggaagcca gaacaccaca caagaactac
    16861 tgcttgggga gcttgcccgg ttcatcgaag aatataggga ttttcgtatt gtctcggctt
    16921 ttcgtacctt tgtaggaatt gctcgccaga tcccatctct cttatttgga tctctcggaa
    16981 aggcgaatga ttttctcgaa tatgcatttc gtatagatcc gaaatttggt cttttcttct
    17041 ggtatatagg cgaaatcgag cttcagttga ggaatattga gaacttgccg gaacacaggg
    17101 agcttttcga tcttgaggtt ctaattggag cctttgtgct ttcctgtttc tagttttctt
    17161 ctttgctgca taaggttcgg aatacgttcc ataaaaaggc ctccagagaa tcaagactcg
    17221 gcttgaggtc tttgatgtcc ataggttttt ctccttgctt acaagcgtat cttattggaa
    17281 gaagacaata tccagaaacc agactcttta gttcattttc actcagcagc gctagcccgc
    17341 aggaaggatc aaagagaaga attcccgccg attcggcgtc ctcttcagca tctaattttg
    17401 catcaataat caggacaaag tgagaaaatc ggccgcggat atgaaggatc aggggagtat
    17461 ctaaggattc aacgagccga tgaaggactc caggagaaaa cttgaatgcc attgaatcga
    17521 ttccctgcct tgcaagaatt gccttgatat cagctagaga aattggtgat gcctttctat
    17581 aagtctgggc tagagcttga tcattttcaa atgctggaat atataaattt gcatatgttt
    17641 ctctctctag ctcctgatcc tcgaagcctg ccaaataggc gctgctccgc attatgttga
    17701 ttaagccagc cgcaaccgca tagccgcaac ttgatgcact tgtctgaagc ggaagcatgg
    17761 cagagacgaa tcctatcgcc atagcaggaa tatgtgaaat ttgcaagaaa agcatctcgc
    17821 gcctccccga agagaagacg cgaacaaaaa ttcatctgct tcaaatatta gaggacgaat
    17881 accctcttgg taggatattg cctataattc tcgcttgggc tcgaaatggc tgaattccat
    17941 actaaaagaa gctcgaccct ggcttacaga tctgaggcta gttgtaaagc cgaacatagt
    18001 cgcaagcggg gcctcggcat gtattatctc ggccacaatc tttgtctccg taccatgaat
    18061 aaagccgccg cgctgagaga caaggctcat aacttctccg acgaattcct ttggagtcag
    18121 aatatcgacc ttcataatgg gctcgagcaa aatagggttt gccgctctgc aggctgcatc
    18181 gtaggccata ttggcgcaag cctcgaaggc aaatacagtg gatgtgagct cgttatattc
    18241 gatatctata agtgaaacac cgatgtccac agctggatat ccatactgaa ttcccgaaga
    18301 gaaggctgac attatgcctc gttctatagc ttcgaagatt tcctgaggca cagccggagc
    18361 tcgcacaagt ttcttatact gattgccggt tcctcttgga agcggttcca cttcgatggt
    18421 taagccagca gtattttctt tgcctgcaat gattttatgg aacttctcgc tatggcgata
    18481 tttggtcgaa atcgattctc tgtaggtgac ctgaggattg ccgatcttgg cttgaacctt
    18541 gaattctcgg ataacgcgcg tgacaatcac atcgagatgc aattctccca tgcctctaat
    18601 gatgatctgg cctgtctctt catcctcatg aaccgcaaaa gttggatctt ctcgggcaag
    18661 aatatcgagg gcttctttta acttgtctcg gtcggaaagg gttctgggtt cgattgcaac
    18721 cgatatgaca ggttctggga agtgcatctt ttcgagaagt actggataac cctcggtacc
    18781 aagggtatcg ccggtctggg ctaatttgag ccctacaaat accgcaatat ctcctgcgct
    18841 aaccgactct ataggctcgc tcttattggc gtgcatacgc agaagtctgg tgatgcgctc
    18901 acgctttttt ttggagttgt tatacacaac tgaggaattg tgcattgtac ccgaatacat
    18961 acgcacatag caaagagcgc cagcctcccg atcatactgc accttgaata ccagtccgag
    19021 aggatagccg cttatctgac atggtatttc tatctcttgc tctcttttgg catggtaagc
    19081 cttgatcgaa ccaacttcgt caggggctgg cagataatcg actatcgcat cgagaagaga
    19141 ttgtacgccc atgtttctgc gcgatgctcc ggcaaataca gggacaaagt gctgagctat
    19201 acaggctttt cgtatctctg caatcagggt atcttttggt atggcaacag cttcgagata
    19261 taacttggtg ataagatcgg acttggacga aacagcatct agaagatttt ctcgccaatg
    19321 ataagctatt tcctgccgct ctggagcaat gggcattttt gtcataaccg aaccatcttc
    19381 gctccagtat atctcttgca tttcgaggag atcgatgact ccctcgaaag aactctcggc
    19441 accgataggg atattcaaag gcaccggatg tgctcctagc ttgcttttca tatcttgtag
    19501 tacggcttcg aaattcgcac caaccctgtc catcttattg atatatgcta tgcgtggaac
    19561 gcgatatcga tcggcttgat gccagactgt ttcggactgt ggctctacgc ctccaacagc
    19621 gcagaatacc gctatagctc catcgagcac acgaagagat cgctcgactt cagcggtaaa
    19681 gtccacatgg cctggcgtgt ctatgatgtt tatttgatga tcgcgccaaa aggttgttgt
    19741 agccgcgctc tggattgtaa taccacgctc ctgttcctgg tccatccaat ccatcgtagc
    19801 ttcgccatcg tcgacttcgc ctatcttata tgtccttcca gaataataga gcatgcgttc
    19861 ggtagttgtt gttttgccgg catcgatatg tgccatgatg ccaatattgc gtattttccc
    19921 caaatccatg gattactccg gagcaaatat aagcattgta atttcttaga acttccgcaa
    19981 gaccggaaga caagaagaaa agccctaaaa accaggctat aagaatatat aggaaaagcg
    20041 aataaaatac aacatcagga tagagcctta taggactgtc actccgtagc atttgttatt
    20101 actggcatct ttttggcgtt tggatctgtt ttcttacagc cgcacatctc atctccttgc
    20161 accaaagtgc cattaaagct tataaggata ctgtgaagaa agttgaatta cttgcgccgg
    20221 ctggatcacc ggaagccctc gacgcggcta ttggcgaagg agcagatgcg gtctatcttg
    20281 gtttgcgctc cttcaatgcc cgtatgcgga caacgaattt tgcctttaat caattcgagg
    20341 ctgcggtata tgcatgccac aataactcgc gacgggtata tgtaaccgtc aataccgtat
    20401 tcgaagagcg cgaatcggat cgcatctacc agttgctaga atatctcgct tccgtaggac
    20461 cagatggcat aattgttcag gatgcgggtg tcgcaaagat tgcgcatgac cattttccgt
    20521 ccttgcctct tcatgcttct acccagatga atatcgcttc ggtcgcgggc tgcaatcagc
    20581 tttcgcgagc gggctttaag agagccgtac ttgcccgcga gctttcacta gaagaaattc
    20641 ggcagataag gcagtataca agccttgagc tcgaggtctt tgtccatggt gcgctctgca
    20701 ttagcgcttc aggactctgc ctgttttcga gctatcttgg aggcaagagc gcgaatagag
    20761 gccaatgtac acaggcatgc agaaggctct acgaaagcga aacagggaag ggctactatt
    20821 tttctccaga cgatcttgaa ctcatagaat atattcccga tctcatcatg gcgggagtgg
    20881 atgcctttaa gatcgaaggc agaatgaaat ctgccgaata tgttggagcc gtggtatcgg
    20941 cataccgctt aatgttggac aattacgaga aagatggcga aaaagccatg ctaaaggcca
    21001 aggctatact tcagtctgat tttgcccgca gaaaaacgag tttttttata tctggcaagc
    21061 ccgaggacta cattcaccct gagcaatctg gaggtacagg cattgccctt ggaagaattc
    21121 gcgatgccag aacaatcgat ggcaagcgct gggccttaat gaacagccgc gaaggcctgg
    21181 ctgaacgaga ttccgtacgt atccatagaa aagacgactc aggccgcata accaccaaga
    21241 tcctggcgat acgatacgaa gtcaatggaa tgcttcttct tttggatgga gactggcgtc
    21301 aaaacgatga gctataccta atccaaacag ccagcatgac gcggagccat aagccggtgc
    21361 tacctaagaa tctcaatagg tttcataaat ttccaagcaa ccacacagcc ccgcgccccg
    21421 caatttcgcg tctcgatggc agaaaactcg agatttcgtt cgaaccggga aactatatcc
    21481 tggtaggcaa ggtagctcat ctgcacgccg ctctgacctt tcgcccaaaa aaggccatga
    21541 tacttttcaa caaactgaat gcagagacaa tgcgcctgga agagaaaagc cttcctttta
    21601 agcgagatag gctcatcctc tggctcgatc catatcttgc cgaatccgat tccacatggc
    21661 ttagcgccga actcgattat tggcttgaaa aaggtgtctc acttgtaatc ctaaataatc
    21721 aagcccattt ttcgatgctg agggggaaaa aggtcatttc aattgctggt ccctggcttt
    21781 acactttcaa taaatgggca cttgcctatt atatcgaaca gggagtgcaa gcctttatcc
    21841 cgccatatga aatttctcga caagatctgt acagactcac ggaatacctc ccggctcagt
    21901 tctttgctcc catagttttt gcatatccag atctatttag aattagagcg gacttatcga
    21961 ggaaatatga caacgcaaaa ttcatggatc gtgaagacaa ctctttcaga ctcataggaa
    22021 aaagagatta ttctgtagtt gttccagata gacctttttc tcttaccgat ttggtaccaa
    22081 atcttaagaa gcagggattc aggcgcttca ttgtagatct atcgaatgcg gagccatcca
    22141 aaggcctata tagggatatc gcacgagcca tggaacaagg tagtccgcta cccaatacta
    22201 cgcgcttcaa ctggaaagat ggtttctgga gcgaagaaag aatgtactaa gagcccatat
    22261 taaaaaagag ctttcataaa taggtgcaat gtgggtctta atgctcttgc ctattccttg
    22321 acgacatatc cgtcagcgta taaagttaca aaataaactg ctttttcata tgtgggcgat
    22381 aaattgaact ggattggcat tttaggggtg gcaaggcatg agtgagtcgg ctggtaaggc
    22441 ctttgatctt ttattttatc tcgcaaaggc aggagagaca gtctcgttag ctcgactctc
    22501 aagagaaact ggtatgaata aggccacagc actgcgctat ttgaatgtcc tggaatcgaa
    22561 aggtgttgta gagcgcagca aactgggttg gactcttggg ctttcgcttt tcgaactggg
    22621 cagcaaggta ccggtgcgtc agctggtagt ggataaagta cgacctatca tagaaaggct
    22681 cgctcgagag accggcgaat ccgtgaacct ggcttatctt gccgatgaaa cggccatctt
    22741 cctcgatcgt gccgaagcaa atcgaagtct tcgtatgcga tcaatgcccg gagacaggct
    22801 gcctctctac tgtacaggag tgggcaaggc gatcctttct cagctcagcg aagagaggat
    22861 ggctgccatc ctaggtccat atcccctgga aaaatatacc gaattcacct gtacagatcc
    22921 tgatgacata atcaaggaag tcagacgcgc cagagaattg ggctatggta tcgacagtga
    22981 agaattcgag gttgggctca catgttatgc aatgcccctg cgtatcccca gttcggactt
    23041 cgttggtgcc ataagtattt ctggtccaac tgctcgtatg aagaatccgg aaatccgcga
    23101 tcgcttcata tatcttctgg aacaagcgat aaaagacgct attgaattgc tatctcctca
    23161 ttatcatttg gacattgtat caaagaaagc caagaattaa agtctatttg atattttaac
    23221 agagagcacc ttttggtcga ccaagcctgg ctcagcctaa aggcgctctc tgtactggcc
    23281 gaatcctaga ataccggtgt ctctattgcg tctcgctttg agtcttcgat aaaaggcgtt
    23341 tcggattttg gggctccttc ctgggctgcc ttgctcatag gtttgaattc cacatgctct
    23401 gcgacaatct tgactcgaga gtgcgacttt ccttcggtat ccgtccaacg atcctgtttt
    23461 agccttccga ctactcttac accgcgccct tttttcagat tttgagcaca ggctgaacca
    23521 agtcttgccc atgcttcgat atcgaaatag gacacttcgt tttcatactt ctgatctgcg
    23581 attttgtaat ttcgattcgt ggcaaccgta aaatcacaga cttgatttcc ggatggcgtc
    23641 gatcggaaat tgggatcgcg gaccaaatta ccttccacca gaatgctgtt taggtgattc
    23701 atacgacctc cttgcgtatt agccgtgcgt ctaaattgca gctgcgtcat cgctggcctg
    23761 catgactaat tcgccaggaa tccctatccc tgattcaatt tttatgccct ctttgctaat
    23821 atatacacat ggaaaacttt gatcggtttc aatctccttt tacgtggcgc tatggaagcg
    23881 aaaaaatgcg agagatatgg agcgagcgca ataagcgcaa actctggcgt catatatgga
    23941 tagctcttgc cagggcagaa tcgcgttatg gccttgtaac gccttctcaa attgccgaac
    24001 tggagagcca tgcagatgat atcgatttcg aagaggccaa tagaattgaa gattcgattc
    24061 accacgacct aatggcggag cttagagttt ttgcttcgca atgtctggag gcaggcggag
    24121 tgctccatct gggagcgact tcgatggaca tagaggacaa tgcagatgtc ctgaggattc
    24181 gcgatagcct ttctcttgta aaagaagatt ttacaaagct cctcctggcg ttggccggaa
    24241 aaatagaaga ctatgcctca ttgccagcca tggcttttac tcacttgcag cctgcagaac
    24301 ctacgacaat cggatatcgc tatgcttgtt atgctcagga cctcgcagag gcctacgagc
    24361 gcctttcttg tgcgattgcg gatatcaggg gaaagggttt caagggtgcg gtgggcactt
    24421 cggcatccta tgcggagctt atcggattgg aaaacctcga tgatttcgaa agagttttgt
    24481 ccgaggatct gggtattgaa tttttcgatg tcagtactca gacctatgcg cgagttcagg
    24541 attaccggat aatttcccta ttggcagaga tcgctgcgat tcttcataag atggcttttg
    24601 attttcgcat tttgcagatg ccgatgctgg gtgaactctg cgagcctttc ggagaacatc
    24661 agattggctc ttccgctatg ccctttaagc gcaacccaat cgaatgcgaa aggatagaat
    24721 ctctggctcg actctttcca agctttgcga atattgcatg ggacaatgcg gctttgtcta
    24781 ttctggagcg tactctcgac gattcggcca ataggcgcat cattctaccc gaagcatttc
    24841 ttgccataga tgagctgttt tcgactatgc ggcatattat agatgggcag atcatcgata
    24901 atgaagccat ccgatgcact ctggatgctt atgcgccttt ttccgcgacc gagcgggttc
    24961 tcatggctct tggcaaacgg ggcgcagatc ggcagattgc tcatgagcgc cttcggaata
    25021 tggcaatgct ggcatggcaa gcagtaagag aaggaaactc caatcctctc aagtctctaa
    25081 tcgagtctga tgaatttatc gcttcattct tatcttcaga tactatcgaa aaactatttg
    25141 atgtcgatat ttatataggt gcagccgaaa aacgagcgct ggccatggca gggcgcatcc
    25201 gaaggctcgc aatggatgaa cattgaattg agcgatatat ttttagatgc tgatattttt
    25261 ctcaaggagt agacatgtta gatttcatca gcaagggtgg acctatccta tgggtcatta
    25321 tgggattatc tttggtagca ctcgcgattg tcatagaacg tctattgtat ttaaagcgga
    25381 tttcgatcga tgaagagaag ctattcttac gcataaagac aagcttgatg gaaggacatt
    25441 tttcggaagc gcttgccata tgcgaccaaa atatatcgcc tttttcgaaa ttgctaaagg
    25501 ttggaatcga aaacagggaa aaacccgaat acttgcagcg cgagcttttg aaagatgccg
    25561 catctctgga atcggtcagt ttggagagag ggcttactac gcttggcaca atctctaaca
    25621 tttcaacctt gcttgggctc ttgggaactg tcacgggtac catgcgcgcc tttggcgttt
    25681 taggaaaatt tggcgctgtc agcgatcctg ccgcactggc aagcggagta gcggaagctc
    25741 tcattacaac ggtaggtggg cttgtggtcg cgattcctgt catcatgatt tacaattatt
    25801 ttgtatcgag aattaacttg atcatgactc gcctcgaaac gcaggtgaat aatctggtga
    25861 gctttatctc gagctatggc ggcaaagaac ccgaaagggc cgaggagaag ccctcgaagg
    25921 aggcatgatg gctcggaacc acaaacttac gccgaagatc aatttcgatc ttacgcctct
    25981 aatcgatatc atacttcagc tggtcatttt tttcatgatt acgacgacct tcaggactgc
    26041 cccgggcata aacttgcagc tcccttcttc caagacggct cagacaatct ccacgcccga
    26101 attgagggta gtagtcgtgt cctcggatga aatctatgtc gataagacta aaaccaacat
    26161 tggaagcctc caatcggtgc tgagtaagcg cgtgagcggc tcggatgcca aacagattcg
    26221 cgcggtcttg gaaggtcgag ccggggcgga atatcagctg attatatctg tgctcgatgc
    26281 attgcgaatg aatggcgttg agaatgtcgg ccttataacg cgaaaagaaa aggtggtgag
    26341 atgagcgagg ccctcaccta cgaagagcga gaacagcgaa aacgcactat tcactcatat
    26401 atcattgcaa ttgcaatata tgctcttctg ttaggcgtcg gcatattatt cgatattctt
    26461 aggccggaga gcattgattt cagcaataaa tccctcattg tgaatattca aggtccagta
    26521 acaaacgaca ttggtaaggg gtctccgatc gaaaaggaag aaagtcttgt tgcggaaaaa
    26581 ccagcaccgc ctccggtcca agttcccaaa cctcaagcag ataccagtgc taagcctaag
    26641 ccggcagagc ctcttgtacc cagtccctct gtcgaggctg agcctacacc gactcccatt
    26701 gttccggaga cagcagcagt acaagagccg gaaataccct ggatccctgg cgaacgaggg
    26761 cctggttcgc gggtcagctc gagtgaatct tcggtgctgg caccgggcga gggacaggtc
    26821 ccatggggca ctggagaggc agtgcgcatc accaaggctg aaaaaggaaa caccgtcgaa
    26881 acgactctgg gcggatcttc ggatagcgtt ggccaaagcc tttatgttcc tatctatttg
    26941 agcatgcctc tgccggagat cgttccggcc gagctattca acgcaatacc ccctgaaatt
    27001 gttcctccaa atacacttat tgcttctgcc gaatcgcgaa agcgagcctt tctcaactat
    27061 tattcatgga atggaaagga gtatcgtctg acaaatcccg tgccattaga aatccgcgac
    27121 aaactctggg agatgttaga ggatgcaggc tataatgtcg aaaaagccga ttataaggct
    27181 ggtcgtttgc tcaatccggt tgtcataggc ttttcgatta ccaaggatcg tcagctgaga
    27241 ggggttgaaa ttctgcaatc ctcgggagat ccggaaatcg acgctgctgt gctctatggt
    27301 ttcaaacggg cgtcattctg gaataagagt ggggatacca ttcaggggcg ctttgtttat
    27361 agattctgat gtatactacc catacctatg atgtgttata atacccgcca ataatccata
    27421 gcattatcat cgggcaggag caggtcgatg attatcaatc tttcatcgct cattgctgct
    27481 ttttcattct gcatctatgt ctttttcata atttttggta tttcaagcag aaaagagcgc
    27541 gtaagtattt catttcttat atatatggtc atgatggcac tctggagcct cggcgccttc
    27601 atggcttata gtaatattgg atttataagc ctatccgacg caatcaaaat aaagttagta
    27661 gggttacttg gggcaccgtt cgccatattt gccacacttc tctatttttc caagagtcag
    27721 aaaaagctct tccgaagttt tctctatatt ggatatggcc tatatgccta tctgctctat
    27781 ttgaattttt ccggctccat tgttgagaat atctggttcg aaggccttga ttatcattat
    27841 acccttgggc aagatgcgct catagtctat tctcttgcgt atgtttactt gcttctggct
    27901 attcttcttt tgctgaagga acaaaaggtc aataagaata agttccaaaa acgcgctctg
    27961 cggctgatgc tggtaggcgt agttgtgctt atggtcggag tattggctta cctatacgaa
    28021 cccgttgggc gctatccagt ggacatgatt gcagcgatga tcaactcgtc gataatattt
    28081 tttactatat ataaatataa acttattcac tattcttcca cagtattcaa tattattctc
    28141 ggctcactcg tggcagcctt ttcgagtatc atatatatgc tgttctttat tttcctcttc
    28201 aagctcgacc tctatgtgcc aaactcgact ttaatccttc tagctctctg tcttggtatc
    28261 tgctctgctc tgatatttgg ccccctgaga acagctatgc agactcttct tgaacatatc
    28321 tcgggtggaa ggagctttct gtattatagg gaactcagga atttttctac taagctcact
    28381 tctattgtaa agctcgagga gctttcgaag ttgattgtag ataatgtgat accagcatta
    28441 aagctcgagt gggcgtttgt tctcttaaac gaatatagat cgcagaattt tagagttatc
    28501 gcttctcatg gcttggattt catacaggaa caaggaaacc agaacccgag taatcaggtc
    28561 cttgtgccca agtcatctcc tttcgtccag gcctatcaaa atcgtgcctc tcaagaagaa
    28621 caagtgtatt cccagtggaa cataggtata atgttgataa aaggccaaaa gcatcagaca
    28681 gtcctggcga gccttgtact tcccttaaag ttcaaggaga ggctcaatgg atttatcatt
    28741 cttggtccgc gcctggagaa ggattattac aataagtatg aactcgatgt attgaaattt
    28801 ctttccgatc aagcctcggt ggcaatggaa aatgccataa cttacgagcg cttgcgccag
    28861 catcaaaagc gtcttcaaga tgctaacaaa cagctgataa accagagaaa gttcgaagcc
    28921 tttttcgatg gaatcgcaac acctatttcg atacaggaca tcaattacaa tatcgtaacc
    28981 gccaactttg ccgctcagcg ctatttcgaa aagactcaga aggagcttat aggatctaag
    29041 tgctacaagg cttatttcaa tagggatcgc ccttgtgccg aatgcctggc tcaggactgc
    29101 cttcatacaa agctgccttt caatggcgaa agacaggaca accgaaccca actgaccttc
    29161 tcgctcaatt tctaccccat tcctacgcag gaagcatctt taggggcttt catcgagctt
    29221 ttccaggata taagcaaaca gaaaagtgag caggaagaca ccatttcaga agaaaaactc
    29281 gctgcgattg gctcgctcgt ttccacaatc gcgcacgaaa taaacaatcc aattggcggt
    29341 attcttggca caacggattt gattctggaa gaggttacgg agggcagcac aatccgtgga
    29401 tatactgagg atatagcccg ttactctaag agcgctctgg attttatcaa tagtcttatg
    29461 gtctattcaa agaagacaca tacttcttta gagatgctaa atatcataaa tgtgctcgaa
    29521 aactctctca aaatggcaat gcggagtatc gattttggat ctattatcgt gcgaaagtcc
    29581 tacgacagcg tcaaggaaat tcttgcaaat tctaccgagc ttcagcaggt atttcttagc
    29641 ctaatcatca atgctgttca ggcaatgcgc agcgacggag ttctgacgct ttcctgtcgg
    29701 caggaaggag atgatgtgct agtcttgatc caagataccg gtataggcat gaggaatcag
    29761 aaattcgata ggattgtcaa tgattttctg acggttgaag aggcgagctc ggttagcggc
    29821 atgggcttga gttttgctaa tcatatcgta acaaaatatg ggggacgtat cttgctgagc
    29881 agccagcctg gaaagggcac cacttttact gtaattctgc catccgcaat ccaagacaag
    29941 gacagaatat ggtttatcaa tgccagcgag ccgaaccaat ttgatgatag ctattatata
    30001 cagcgcaagg tgctcgtggg agaaaaaggc tacaaggaag aaaccataca tcgcaagtgc
    30061 gacgagacgg cttttcatat ccttgcttat aaagggcttc agcccatcgg taccgtatct
    30121 attcatctga gcgaagcaac tgggcatatt cctatcgagg agaacttcga aatcggcccc
    30181 tatctcgatg gcgctccata tgcggagatc gacaggctag cggtgacgaa ggaggagcgc
    30241 cagggcttta cttcattcga aatcatggtg ctatcttatc tatatgttcg cggtcggggc
    30301 gcaaaacaga tttttataga tacctttgcc gacgaaacag gtctgattca aatgttcaag
    30361 aaacttggct tcgagataat tggaggttac agcaatcctc ttccatgcac tgtcttgatg
    30421 atgaatcatg cttcgacata tgagcaggaa acgaccaaga atcggctctt tatcaagaaa
    30481 actctctctc gtcttgtgcc taaaatcgac ttttccgcag atgagatgtc atatgtaatg
    30541 aaagcaatag acgagataag cgccatatct ccaggcaagg atagtgtaga cactaaagag
    30601 atatcgtcat acaatctatt atgatatgaa gcccgaagat ttagcagagg gactaaacac
    30661 cattttagct cactcaccgg ttttcccgga taagtgcctc attatcgatg tggttgctaa
    30721 tcccaaagct ggtggtttta gccggatcca ccattcaaag aagcgaatga gagagctaaa
    30781 agagatggtg agccggtcac tcgagctgcc tctacgcaag gctccgttaa gcctgaaaat
    30841 gcacctgacc gagagatgcg gacacgctgc aactatagtc cagcgcatac tggagcattc
    30901 tccatccaat ggaaaagatt cctaccacat tatcataaca gccggaggag atggcacaag
    30961 tctcgagact gctgaacaac tcacattgct tccggaatcc gagaaggatc gctttggatt
    31021 agttagattg ccctttggaa ctggcaatga cggctcggag ggaagagatc tctttgttgc
    31081 tctagagagg ttcctcaatc cagcaattct cgagcgaaag ccggcgctaa aaatcacccc
    31141 aagctatgaa ggaggagcgc tgcctcgcta ttctttcaat atcgccagca taggtctgga
    31201 tgcctatgtg gcggagatga caaatcgcct taagcgtcat tttcctgggg attcctacaa
    31261 gttttgggtc gatataggga ctatgttcta tgatcgtgtc tataaagtgg ttaacatggg
    31321 tctcagggct tggaacagag gcagacttgt actggattcc gagcttcctc gactcttggt
    31381 agccatggga gcttcgggca accgatatta tggctcacat aagagcatac ttcccaatga
    31441 gcacaactgc attgccatat ctcagaccag cctctttaga aagctcattt taaaagggtc
    31501 catagaaaat gggaagcacg aacatatccg agaacttaca cattttacag cggacagact
    31561 ccagatagac taccacgaga gcattccctt gcagtgcgac ggagagacga ctctccttgc
    31621 aaagtgtgac tttccgcttg aaatggagag acttcagaac gcctacaatg tgcttgtgtc
    31681 ggtgtaaagc gcaaaacgcc ttattttgga atagattgcg ccaacaggac tgagggtcga
    31741 ctgcagaagc tcaaaagaat cgatatatag gctcagcatt gggggtttag ggagcgacga
    31801 aagattgggg gcataaggca gcttgagccg cgcgagggta agatgagcct tccatgggcg
    31861 agcatcggtc tgtgctccaa gttgggctgc tatctcgcgt atcctatcta ttaaaatcgt
    31921 aagcggttct gatggctcta tacgcgctgc aagatagagc actcttggtc tattcatggc
    31981 tgggagaaca gacagccctt ctgtagttat cctgagggga gggcagagat tagccgcatc
    32041 ggaaagcaga ttttcaagct ctttcgcctt ttctacttct aattctccaa agaaatgaag
    32101 agtcagatgc agattctgag ggggaaccca agaaggacgg gcaggtctaa aagccgtctc
    32161 tatagagccg cagtaggttc ctatctgatt ctggacagca gccggaaaag gcagggcgac
    32221 gaagagacgc atggccttat tatgtcagat aatccttcct tatgcgagac atagactcaa
    32281 aaatattccg cttcttttgg ctcgagccct cggtcaaatg ttcgaggagt tctcttgctt
    32341 tttttcgttc tccttctaaa ttgaatgagc atgtatatgt gtaatccgac aaacccaatt
    32401 catcggcaca agctaaaact tgcttttctt cacacagagc aaggggccgg atgactgtta
    32461 tggggtattt tctatagggc atgacaggcg gcatggttgc aaattcgcct tttcttagca
    32521 tgttcatgag cagagtctcg agaatatcgt ccatgtggtg gccaagagcg agcttattga
    32581 acccatgttc cttggcaaat ttcatgagct ctgatcggcg ctgggtggta caccagtagc
    32641 agttcattga tcttcctggt tttagtctgc ccactacagg tatctcaaga cgcgtgtatg
    32701 gaatacccca tttggtcata tacaaggcaa gccaggaatc gtcccgcctc tgcgaaaggc
    32761 caaaatcctc aaggtcggtg actatatgac aggcggcaat ttcgaaagga gtttccaacc
    32821 attggcgttt cttggacaga tcccaagcga ggcaacagga atcttttccg ccggaaaccg
    32881 cgattaggat tctatctcca ggctgaatca tatcatagcg gcgcagagca atctcgcaaa
    32941 gcttggaaag agtggcacga gcgtttttca ttttatataa atgcagatca aggccgctca
    33001 aaggtcatgc cagccataga tgctgactcg atttgcaatc ggaatctttt tcttttgaag
    33061 cgggatctgg ctctttgtgg ccttcggagg ggccgatgac cgaattgatg aaagaaacga
    33121 gcacgtccag tgcccgctca ttgaatcctc cctcgggaat gattatgtcg gcgtaagcct
    33181 tggttggttc gataaagtcg aagtgtccag gccttaccac tgtcagatat tgttctacta
    33241 cggaatctac ggtgcgacca cgctccttga tgtctcgcga taggcgtcga ataaagcgga
    33301 tatcatccgg tgtatcgaca aagatcttta gatctatgag gtctcgcact tctttgttag
    33361 tgaagatcat tattccctcg aaaatgacaa gcttcgaggg ctgaatatgg atagtctgat
    33421 ctgttcttct atgatggacg aaatcataga caggcatgtc gatgggttgg ccattcttta
    33481 gagcggtgag atgagagatc agaagctcgt tgtcgaaggc atcaggatga tcgaagttga
    33541 atgctgtgat gttgaggtta ctgatgaatt ctgctgactt gtagtaatta tcctgtggca
    33601 ggaagacaaa gtcgctgaca atttcggata tttttcgaac tatagtggtc ttgcctgagc
    33661 cggaaccacc ggaaataccg attatcttga ctgctggcat atcctttttt accatgcctt
    33721 ggctttatgg taaagtggaa ggcctatcca ttctgcgcca tccgaatgag catttcctga
    33781 atttcatcga taatgaagtc gggagtagag gcacccgcgg taattcctat gcgatcataa
    33841 ctcaccatct cgggcttcag ttcgtctgcc gtctctatat gccaggaagg aagaccgaag
    33901 cctatggcac tcctatagag tctctttgta ttctcagagt tcttgcctcc aactacaagc
    33961 agcgcgtcca cctgtgtgct gagttcagcc aatgcctgct ggcgctctcg cgtagctgga
    34021 catatagtct tttctgcgat catctttcta acataggatt gaaaaatatc gataactgta
    34081 tcgtagtctt cctgacggat tgttgtctgt gcaatgaggg cgcaaggctt atctctgagc
    34141 gatctcgcca gggcaggggc tgtttcgggg ctatctacaa cgagagcatt caaggtatgt
    34201 cccagaatac ctgcaatctc accatgtcgg gaatcgccga caagcactac ttgccaaccc
    34261 aatctctcgt aatgccgggc tagaccctgg ctcttgatga ctcggggaca ggtagcatcg
    34321 attattcttg cgccaagcac atgtaggcga gatattgcgg ccagcgatgc tccatgagcc
    34381 cttattatga caataccatc tctcaggttt gggagttcat ctagttccga ctcgattgtg
    34441 cttggatcca gaacatgcac accaagcgat tcgaggtcgg caactgcctg aggattatgg
    34501 ataagagggc catagctata gacagggcgg ccggttcgag cagctctttc ggcggctcga
    34561 tgcgccagct cgtccgcgcg tcgcacgccc atacacatac caagaattct tgctcgaaca
    34621 atctgcataa ttggatctta tcagattttg atagccattt ccacaatgcg cattgcggtt
    34681 tttttgaaaa gtgctatact actatttctg gaaaatttcg ctatccggaa aaggagaatt
    34741 catggcaatg cgcaaaacta ttgtcgtggc gcttggtggt aatgcgataa tcgaagaagg
    34801 cacggaagga acgatagaac agcagtttga aaatacccga aagagcatga aggcaatagt
    34861 tggcatgata gccgaaggcc acaaagtggt tctgacacat ggaaacggcc ctcaggctgg
    34921 tgtacatctc atccgaaacg aagcagcttc cgagcaggtt cctccatctc cactaaatgt
    34981 cattgttgct gatacgcagg gttctatggg ctatatgatt gcacagagtc tttccaatgc
    35041 actgcgcacc gtaggcatag acaaggatgt ggtaaccgta ataacgcagg tagtagtaga
    35101 cccaaatgat ccctcaatgc tgaatccaac caaatttgta ggtcctttct acaaattcga
    35161 ggaagtagaa aaactgcgcg cccgcggctg ggtgatcaaa gaagatccaa tgcgcggtta
    35221 taggcgcgtt gtgccaagcc caaaaccact cgatgtcatc gaaaaggaga caatcaagga
    35281 ccttatagac gatggcaaga tagtcatcgc ggttggggga ggtggaattc ctgtcaagcg
    35341 taatccggat gggaccctct caggcgtaga tgcggtgatc gataaagaca gggcttcgtc
    35401 gctccttgca agtctaataa atgctgatga gctcatcata cttactggtg tagataaggt
    35461 tgcaatcaat ttccgaaagc ccgaccagcg ggtattcgat catctcagcc taagagaatg
    35521 cgaacaatac atggctgaag gtcatttccc gaaaggttcc atggggccca agatcgaagc
    35581 agcctgcgac tttataagga gaggtggcgc aaaagtcatt ataacttcca tggaaaacgc
    35641 aacggcggca gttgatggca aggcgggcac tgtgatctcc gcttagcgca acttgaccat
    35701 tcgagctgct ccatggtaat tttgctggaa ttctttttcg at
//
LOCUS       DAOMZL010000006        34974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_906_length_34974_cov_19.128326, whole genome shotgun sequence.
ACCESSION   DAOMZL010000006 DAOMZL010000000
VERSION     DAOMZL010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 34974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34974
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_906_length_34974_cov_19.128326"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(295..1962)
                     /locus_tag="PLQ30_01180"
     CDS             complement(295..1962)
                     /locus_tag="PLQ30_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPW01087.1"
                     /translation="MDLYAHEPLGFDGILVHIEADIRNGIPAVEIVGLASTSVREARE
                     RARIAVRNAGFEFPQDRILVNLSPADLPKEGSAYDLPIALKILEKSGQIGDLPICAMA
                     MGELTLEGEIRPVRGILPAVRSAASHGIRICILPFENAQEARILKAVQVWPIRHLAES
                     NRILIEIGKGKTPQGDVQEGQFSQGDLPIGQEKQIPPRSPSLWLDLNESRETYSDFRG
                     DERLMRALVIAAAGGHNMFLAGPPGAGKTMAARRFPSILPDLSEQEALDTASIYSLWG
                     SLWTCSGRRPPFRAPHHSASMEGILGGAKPLRPGEVSLAHHGVLFLDECPEFRRDVLQ
                     GLREPVERGYVDIVRAGRVLRFPSDFQLIMAANPCPCGNLGMRGKTCICSPEEIRRYW
                     KKLGGPLLDRIDMRIAIQPPKASRLIESRAVDVDLLRDKVKSAREIQNMRFKRTNDGM
                     SKKEKENMGSSRSMMFSEANQILTCNNAHIPAGIVNDICHIAGSAEKAFLSGMANYSL
                     SARAGHSILRLARTIADIDGENEITESAIEEAIEYRQFGDGDAVWPF"
     gene            complement(2033..3751)
                     /gene="pheT"
                     /locus_tag="PLQ30_01185"
     CDS             complement(2033..3751)
                     /gene="pheT"
                     /locus_tag="PLQ30_01185"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPW01088.1"
                     /translation="MPKIEVNEEFFFQLAGQSWSNYEDLEQDLTIAKAELDEWDRIPS
                     DAGERMIKIELNDTNRPDLWTTAGLARQLRIYRTRNIPSYPFFASKGKSIDAAYRVVV
                     EKSVREVRPWIACFVAKGKPISDALLKDMIQTQEKLAWNYGRKRKGVSIGIYRTGLIE
                     WPVRYFGVEPDSVSFVPLQETRSMTLSQILKEHPKGVEYGSILEGKTIHPLLTDSKGS
                     VLSYPPIINSADIGAVQVDDSEVFIEVTGTDYPSVALSASIMACDFYDIGFEIANVRV
                     DYEYDSPFGSSVIFPYYFQNKVTLSLIEANRLLGDSIDLKKAQDALARMGLGAYCSDG
                     LTLHVTPPEYRNDFLHPVDVAEDIMIGLGIENFAPERPSDFTIGRLTPIEVFSRKAKD
                     IMIGLGYQEMIYNYLGSGIDYAEKMQIPVGQLVKIANPMTENYEYVRNSPIPGLLQTE
                     SVSAKASYPHRTFEVGKVAIKDPEENYGVVTRQHIGFLISHAQADYNEIASHVATLMY
                     FLGKNYTVRESADPRFVPGRQVEVFLEKESVGVFGEIHPAVLEAFDVMMPCAAAELDL
                     DSLLIG"
     gene            complement(3768..5330)
                     /locus_tag="PLQ30_01190"
     CDS             complement(3768..5330)
                     /locus_tag="PLQ30_01190"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPW01089.1"
                     /translation="MIDQSAARILHPLEVRIILAFDEGEIIDSQRVKQVLGFREGQEQ
                     QAFSWLLAKGVLEEAERRTEVFYELTPLGQEWHEKGIPARRIFSLLKDRGPMSLPEIA
                     QTLGFDQKTVGSAFGSLSKEAVCAIDEARRARLAKNEFPASLSEIESLLALAASSGGS
                     VSEKALSDSQRALMGQIAKKRGAQDSPFRITDRVRVLYRVTPIAKEYRKAISTLGMTG
                     EELGAITPEMLEKGTWKKGHFRPYNVQIPPARVIPGRRNAYVEFLEGVKDKLVSLGFV
                     EFDGNLVETEFWNSDALFMPQFHSARDIHDVYYIEEPTHAKFIEEPYLSNVAAAHENG
                     GNTGSRGWQYQFDRDFTRRLILRSQGTVLSARQLPKAEVPGKYFGIVRCFRYDRVDAT
                     HLSDFYQTEGIVLGRNVNLKTLLGFLEMFAVEVAGAKEVKYVPGYFPFTEPSIEVHIK
                     HPVLGWFELGGSGIFRPEVSSSLGVEVPVLAWGIGIDRMALMALGLNDLRDLFCSDLE
                     SVRMRRSQYKIQ"
     gene            complement(5382..8387)
                     /locus_tag="PLQ30_01195"
     CDS             complement(5382..8387)
                     /locus_tag="PLQ30_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPW01090.1"
                     /translation="MYFNELVSTYDSQRRERNIYHDLMRDRVREILLVGSLYDSFVVE
                     SDGVLTEQIYGEYFKLNLNAVPRITCAYTEESAMDLFRDGRFDLVVVMASTEFNMPLR
                     LAVSMKSTWPDIPILLMVTDNSSLAMLDMSRPELAVFNRIFVWNGYSKLFVGMIKYIE
                     DWRNARADTHAGQVRVILLIEDSVRYYSRYLPVLYRVVLKQTQALIEGERIVETNKLM
                     SIRARPKVLLANTYEDALEVFESYKPYLLTVITDIRFRRNGEFDDNAGFDFLKFVKQE
                     LPDLPMLVQSSETNVREKAYAFGASFIDKNSESLEMELAAFFEANLGFGNFVFRMPNG
                     AEIGQAGNMSEFIQKLNEIPIESLLYHAGHNHFSAWLMARGEINVAKIMKPYRITDFG
                     SPEELRQFIVNMIDQIRSVHSRGIVPELDPEMVKHSRYLCKIADGSVGGKGRGLLFIR
                     SLLENFDFPQYVPGIEISMPNTVFIGIDEFEHFLESNGLWSWAYYDKEVQDLRSVFVQ
                     KPLSQKLENNLRQLLSLSKRPLAVRSSGLFEDMLMVPFSGVYDTYILPNNHPDSEVRF
                     KQLSTAVKLIYASLFSKEARAYFEATNYNLEEERMAVIIQELAGNRHGDYFYPHISGV
                     AQSYNYYPISYVKPDDGLCIAALGLGTYLVTGGTAFRFCPRYPKLDIGPLEYISENTQ
                     RYFHVLSLRDKDPNLLKGEMASLFELPIDAAESDRYFSMFASTWDSLDQRFVPGVSTD
                     GHRIIDLANILKHEAYPFAKAIDIALDIGKRSMGTPVEIEYALNFDDDKQEPTLYLLQ
                     LKPLIHLEDQIEIDLSSIQKDACFLLSRNCMGNGRDLSIHNIVWVDPRKFQRNETLEI
                     AAEIEELDVLARKENFDYLLIGPGRWGTRDRWLGVPVSFDQISHAKVIVEMDLPGFVV
                     DSSLGSHFFHNLTTMRIKYMKINQGSADDHIDLEWLYSLKPRVGTRHCVLTELNTSLD
                     MRFDGRSGKGAIFKSTESSNLKRAIETGN"
     gene            8521..9462
                     /gene="aroF"
                     /locus_tag="PLQ30_01200"
     CDS             8521..9462
                     /gene="aroF"
                     /locus_tag="PLQ30_01200"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPW01091.1"
                     /translation="MFKNIKLAARNPGQKTTVIDISGVKVGDDLVVIAGPCSVETEKQ
                     TIETAIAVKNAGAKMLRGGAFKPRTSPYAFQGLGLKGLKILDKARRETGLPIVTEVVD
                     TRDVSWVGEYADVLQIGARNMQNFSLLREVGKSGKPILLKRGMYSTLEEWLNCAEYIL
                     AEGNPNVILCERGIRTFEAYTRNTLDLSMIPAVHRESHLPIFVDPSHGTGILSLIEPM
                     SLAAVAAGVDGLEIEVHIDPKSALSDKDQQLTTVMFESLMKKLRGLKHFLDESNVASD
                     ESSDEYASEECISSSHDGTKASNSVGTLTDAFSEAVD"
     gene            9473..10531
                     /locus_tag="PLQ30_01205"
     CDS             9473..10531
                     /locus_tag="PLQ30_01205"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HPW01092.1"
                     /translation="MQRNQSIIRFGKTSELLSLIPIEKAIFVTSETLYGQALEMLSSS
                     SLDAKEGLCWIKVPDGEACKNFSVLETVCRQMIDLDANPDTVLVAIGGGSLSDLVGLA
                     AHIWMRGIDIALVPTTLLAMIDASIGGKNAIDIGPTKNLIGSFHIPRLILCDVGWLET
                     LPSRDLLSGMAEAIKHAVIDSEEHFRFLEGIANSEESLTEINAELWEELVQRSQQVKI
                     RYVDADPYDNYLRHALNYGHTFGHAIELITEIPHGFAVATGIGMANDLAVSRGALDPD
                     IAARINSLLHSIGLPANVAEVSAIAGCNIRPAAIYDLIRADKKRRGESIDLALLRNIG
                     DIRIEPVALQYIAEFLGA"
     gene            10559..11581
                     /locus_tag="PLQ30_01210"
     CDS             10559..11581
                     /locus_tag="PLQ30_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPW01093.1"
                     /translation="MNTIGKIFCISLYGESHGKAVGVVIDGCPAGISLTEEDFAEDLN
                     RRKAGAEGTTTRTESDKPILLSGVFDGHTNGAPIHIAFRNENIRSSDYEQFIDIPRPG
                     HADFSAMMKFQGWQDPRGSGHFSGRVSIGLVAAAVVAKKILNSFSDIVFRTRLLEAGG
                     SRDIDKAIYRAKESGDSIGALVEIRVSGVPAGWGEPFFDTAESIISHYIFAVPAVRGV
                     EFGDGFAAAKMRGSQHNDPFIDLHGRTARNGAGGINGGITNGNDILLRVAVKPASSIA
                     STQNTLDFSRDEMVNLEIHGRHDACIALRGAVVLEAATACALADLALVARAEAPFISN
                     IPWRKS"
     gene            11578..12636
                     /locus_tag="PLQ30_01215"
     CDS             11578..12636
                     /locus_tag="PLQ30_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HPW01094.1"
                     /translation="MNLDDIRSDIDRIDAKILSLLNERMEKSLIARHFKAVALDPVRE
                     QIVLEKVRRSSQCLLDPHFSVHLYEQIISECRHLQDAGLKTVAFQGEHGAYSEVALRL
                     LMPTAATIPCCEFSDVFDGVEKGIYDYGIVPVENTLGGIVGPVNSILIYTRLKIVAAI
                     DLPIRHCLLTIPGADHRELRIARSHTQALTQCRNFLLRNHLDPEPFFDTAGAAKALAE
                     ERPKGVSVVASKFAADLYGLEIIKEDIQDSPHNRTRFFIIATEENGVEGDKCSAVFTA
                     GNKAGALFSVLEVFAEENINLTRIESVPDVPEKYAIFIDFEGSLSSAPVQRALEKVPE
                     IAEDFKILGCYHEMRVES"
     gene            12633..13409
                     /locus_tag="PLQ30_01220"
     CDS             12633..13409
                     /locus_tag="PLQ30_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HPW01095.1"
                     /translation="MRIAILGAGRMGAWLTEELCWNNEVMVHDSDLLKMKYFIKVHRA
                     LAIEEFADFKPELFINCVPLGLTLDSFDAVLPYLPSQCIISDIASVKTGFREYYENCR
                     HPFVSSHPMFGPTLANIRDLHEESAIIISESSKEGADFFRRFYSSLRICIFEYSFEEH
                     DKTVAYSLATPFASTMVFAACMKKQDAPGTNFKRHLAIAKGLLSEDDRLLTEIMFNPY
                     TIRQLELINSKLAYLTHIIRQRDYDEMKKFLDKLRANIEQ"
     gene            complement(13515..14855)
                     /gene="aroA"
                     /locus_tag="PLQ30_01225"
     CDS             complement(13515..14855)
                     /gene="aroA"
                     /locus_tag="PLQ30_01225"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPW01096.1"
                     /translation="MGTRVAKPGKVCGSIQAPASKSALQRAIACAILARGISHIEASP
                     VCSDARAAIIIAKELGAKVKEEKGILEIKGSPLFHKDAKGKLATTSQKVCYPGLKNNG
                     FQPCLPLVLPCGESGLCMRMFSPIVALLDREVELRGEASLSSRPMNMVEDSLKSLGIS
                     CISRGGVQPIRIQGPLHPLKSRIDALGSSQFITGLLMALPLLDGDSELIVDNLVSSGY
                     LDLTIEICAEFGVEIKRNANGAGFWIPGNQVYKATDLIIEGDWSAGAFLAVGAAIAGS
                     EEGLEIHGLHQDSTQPDRAIVDVLTEAGAQVQFKGSDCIVRASALAPFDFDATNCPDL
                     FPPLVAMAAAIQGRSCIRGTKRLLSKESNRSIALCKTFRKLGVDVTLQNDAMYIVGGS
                     IHGATVESYGDHRIAMAASIAALVASGEVSIEGAECVSKSWPGFFDDLSSIVVS"
     gene            complement(14881..16227)
                     /gene="gdhA"
                     /locus_tag="PLQ30_01230"
     CDS             complement(14881..16227)
                     /gene="gdhA"
                     /locus_tag="PLQ30_01230"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPW01097.1"
                     /translation="MNSYIKEVLDGLHARYPWESEFLQATEEVLESITPLIEAEPKYR
                     QQKILERIVEPERVVIFRVPWVDDKGEYHVNRGYRVQFNSAIGPYKGGIRFHPKVTLS
                     TLKFLGFEQVFKNSLTGLPMGGGKGGSDFDPNGKSDNEIMRFCQSFITELFRYIGADT
                     DVPAGDIGVGAREVGYMFGQYKRIANEFTGVFTGKGLTYGGSLVRPEATGFGAVYFAE
                     EMLKTMGDTLEGKTVAISGFGNVAWGACMKASQRGAKVVTISGPDGYIYDPDGVGTKE
                     KWNYLNEMLIIDRNKVESYVRKFPNAVFHEGKKPWEQKVDLAMPCAIQNEMSGEDARM
                     LVENGVKVVVEVSNMGCKPEAWKYFIEKKIPFGPGKAANAGGVATSGLEMSQNSMRMS
                     WSAEEVDRRLHEIMTNIHKSCIETAAKYGFEGNYVVGANIAGFKKVADAMIAQGLV"
     gene            complement(16347..17147)
                     /locus_tag="PLQ30_01235"
     CDS             complement(16347..17147)
                     /locus_tag="PLQ30_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPW01098.1"
                     /translation="MNILSQLFGVHGKTVESKDEVVEEQREMIEGVEHLPETIVRDVM
                     VPRTDTVCVEDISPIDEILETLVDSGHSRIPVYSGTIDNIVGILYAKDVLAALVKKKP
                     LEVKSLMRLPYFVPETKRIDTLLKEFKRRHVHIAIAVDEYGGTAGIISLEDIIEEIVG
                     EIQDEFDEEADQVVPIGKNRWSCDARIHLEDLNDAIGAELPYEEYDSLAGYVFDLFER
                     IPSKGEQVTVGDLQFTVEEMEGHRLTKICIEKLDMSQEDSPFTPMHVS"
     gene            complement(17144..17605)
                     /gene="ybeY"
                     /locus_tag="PLQ30_01240"
     CDS             complement(17144..17605)
                     /gene="ybeY"
                     /locus_tag="PLQ30_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPW01099.1"
                     /translation="MNTNEVFYSMIGIDEPSWAQRSADYALSVLDALGKKDWQISLTF
                     CDDNTIQTLNRDYRHVDSPTDVLSFVLGEYEPIEGSESIYLAGDIVISIPAIERNAEE
                     YGVGEDEELRRLIIHGILHLTGMSHEDDSPDQPMLLLQEQLLKTLGGNVIL"
     gene            complement(17602..19803)
                     /locus_tag="PLQ30_01245"
     CDS             complement(17602..19803)
                     /locus_tag="PLQ30_01245"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPW01100.1"
                     /translation="MKKNNRESWFFELKKSLRSVQPPLWFFFVAIAIAAIGLILVGPS
                     SISSRSSLKAIKVGEIAETDIYAGKDTIYIDKEATQRKIQAEERLVLAVFVMDDKITA
                     SIREKAEEFSSYYLQLLGSESGSIDALSLMLSSRFPDLLQSDRYKTLVSAELNAQTFV
                     YLSDIVESLLSKGIVSVPEGVFDRFNPDYYELVRMLNGREESEKIAVRNMITLANLNN
                     RLEDEMDARHIPESLKQYVRLLGKALLRENAFFDQNLSDKRLAAARARVEPVTRFVSR
                     NEILVRKGELITPEVYQQIKTIRSSILVSDIGVLLTGLGLLLIASLLGFILSRMPDLS
                     DFPVDRSSLIFLLISFLILFYWILVIQNLLNGKSPSHGALFVPASLFAGLVSLLYGAR
                     TGIFFSLVSFFLTGAATNLNAQFMIGILLAGISATLIMRTAHTRIMLARAAVLQALVQ
                     AILAIILLLNQQPSAAEVFQSMGLGALNGFAGGSFILLLLPLFERLLDRSTQFRLMEL
                     ADINAPIMKEMLMKAPGTYAHSMNVANLAEAAAKDIGANPLLARIGAYYHDIGKIDHP
                     EYFAENQQGINLHDDINPTLSASIIRRHVKDGIERAREIGLPKEVIEIIGQHHGNSVM
                     EAILVKAKNNVAQFDLQSYSYQGERPKSKEAGIVMLADSIEAASRSLKNPSAAKLEDL
                     IHQLIIKKLTSGQLSDSELTFNDIEVAEKAFLRILQSQMHTRLEYPEQRKQ"
     gene            complement(19790..20782)
                     /locus_tag="PLQ30_01250"
     CDS             complement(19790..20782)
                     /locus_tag="PLQ30_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPW01101.1"
                     /translation="MSPSHIVLEDTEVFAELCGANDYNLSVIGSLLGTHVLTRGNELF
                     VDSDDPGILNLFSRIISAIGDSIKEGVPATPELIIALHAELTPDGSQSFKATIDNADE
                     ENESVISRFLENCIQIPNGFGKVFPRSSGQSLYLKGIASHDLIFSIGPAGTGKTYLAM
                     AYALRELLTRSKRKLILTRPVVEAGESLGYLPGDLEQKINPYLRPLYDAMEALVPYET
                     IRRLEESRAVEVAPLAYMRGRSLANCIVILDEGQNTTKEQMKMFLTRLGEHTKAIVTG
                     DITQIDLPKKTDSGLIHAITLLGNIDEVFISRLDASDVVRSSLARKIIEAYEKE"
     gene            complement(20958..23933)
                     /locus_tag="PLQ30_01255"
     CDS             complement(20958..23933)
                     /locus_tag="PLQ30_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01102.1"
                     /translation="MRRIIGIIFIFRILLVPCAAQAQIQNDNRAYKPEEVVQKSDSDI
                     TLTIEQAASAEYFSLENGTKELRLTGPLIIRLIDSGGVTHSIKAQYVVYNRDTKEVKA
                     YGDVEYTRRTESKTDIYKGESIAVDLDDNSGVFIDGSFDMESTTPGSRKLIVHFESLI
                     SRSEEVIALANGSLTACDAIKPHYILRAKKIWLFESGDWAASNAILYVGAIPVIWLPM
                     FYYPSKASEFHPVVGFRSRAGAFLQTTTYLKGAQSSEVQKSSLLSTTEGTSGSFGSYI
                     SKTKPADESKNQTNIAILGDVYSALGVFAGLRGELANTYPYDISWLIGAAFSRSVFLE
                     STGYYSPYDSAGDYASVWNRWKLSSIDLPMRILMKFEASSKKNKAGIKWQISLPFYSD
                     PFVEQDFMDRNESYDFFSILGSSSLKTIDERSSFSQKASLSWSWNPFIEKNSFAFTLS
                     NLSSSFNWKRKYASTAGMNPTEKRLNAVDPQRYFFYPDSARIIDTAFSMSGTILQSKN
                     MSLKWTNTNSSFLEDRFYNAKWQKPQDIDFESWYWLYGGRSNTNLSSTYSIDSVGLTF
                     QISTGILGQLQYRPYLYDERVNATTVHPFLIADYSYNTANWNTGTKLSWEPFIKSDTF
                     SASKVSYSIQGKVAKLAYEGLDGSGVDAYPIYKLYWLSWDPNMISDHSILAELAAKLG
                     STTERLSFKATLPPILESYTLSGSSALGFATVGASYVISRKNSLEDLKSTSLMGNLAL
                     RPIKKIEISANAAWDFDTQAPLSLSANLSAWSFNARFVAQKANGYVFKNSSWIQDGTQ
                     YFRPSTLSLSWKPKMRYSSPLFSTEMMSWYVEADGLLTLNQNLIQFTNSTFGANLGLS
                     IKNSAGLSLGFTLSSVNSSFWRYYASLFPKSDDFDPEDYSKNLFADLWDSVSIWDANK
                     LKNTLFKLQGLKLELSLDAHDWVLSANILAEPTLVTASSTQPLPHYEMEVAFNLAVTW
                     KDISAIKSSINYSEGAFKQ"
     gene            complement(23993..26815)
                     /gene="uvrA"
                     /locus_tag="PLQ30_01260"
     CDS             complement(23993..26815)
                     /gene="uvrA"
                     /locus_tag="PLQ30_01260"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPW01103.1"
                     /translation="MDKLIIKGAREHNLKNIDLELPRNRLIIISGLSGSGKSSLAFDT
                     IFAEGQRRYVESLSAYARQFLGRMDKPDVDYIEGLSPAISIEQKTTSRNPRSTVGTVT
                     EVYDYLRLLFARIGVPHCPSCGREIREQSVDQILDVILSWPMGTKIQVLAPVIRSQKG
                     EHKKILEDALRQGFLRARIDGQFIDIESPPVLEKQRKHTIELVVDRILLSEESRRRVA
                     EAVETSLGIANGIVIIVREMDGQRKEDFFSQKGACPICGISLPEMEPRLFSFNAPQGA
                     CPACSGLGMNMEFDPSLVIPDRSLSFNEGGCLAYNPNSAWNRSRFEALADHYHFSLDT
                     PFDKLPSYVMNAILYGSDDNIHVRYNNRDKTGYFEYESRFPGILADLRRRYMESTSND
                     IKDWLESFMVEKECEVCHGKRLKPEALAVTIGSKNIYEISCMPVEDSVQFFKSLRLTD
                     TEAQISKLILKEIIARLGFLQNVGLEYLTLERQASTLSGGEAQRIRLATQIGSSLVGV
                     LYILDEPSIGLHQRDNQRLIDTLTYLRDLGNTLIVVEHDEQMLRTADYIVDLGPGAGE
                     HGGYIVAQGSVQEVVANPQSITGQYLAGSLKIEVPIERRKGNGACITLKGASEHNLKN
                     IDVKIPLGRFVCITGVSGSGKSTILSDVLYPAISNRVMHSAIREGSYKSLEGIEYIDK
                     IINIDQSPIGRTPRSNPATYVGVFTPIRELFASLPESKARGWKPGRFSFNVKGGRCEH
                     CQGDGTIKIEMNFLPDVYITCEVCHGKRFNSDTLDVRFKGKNIADVLNMTVEEARTFF
                     AHIPSIAHKLQTMVDVGLGYIRLGQSALTLSGGEAQRVKLSLELARRATGRTLYFLDE
                     PTTGLHFADVRQLLEVIRRLVDSGNTVVMIEHNLDVIKQADWIIDLGPEGGERGGMVI
                     AEGTPEEIALCRDSYTGQYLRRLLNI"
     gene            complement(26890..27996)
                     /gene="ychF"
                     /locus_tag="PLQ30_01265"
     CDS             complement(26890..27996)
                     /gene="ychF"
                     /locus_tag="PLQ30_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPW01104.1"
                     /translation="MPLNCGIVGLPNVGKSTIFSALTRAKVDTANYPFCTIDPNVGIV
                     PLPDKRLDRLFDIFKPKRKVPAVVEFVDIAGLVRGASKGEGLGNQFLSHIREVGMIAH
                     VVRCFDDPNVVHISGKIDPADDIEIIHTELALADLESIDRRLDKLSRQVKVQDPNLKK
                     DIEKEIAVLQKLRPILEEGRPASTAILEPEEVDYARRSFLLTLKKELFVCNVDESGMA
                     NNKYVDIVKSIAEKQGSEAVVICGKYEAELADIEDSEERAEFLHSIGLEEPGLDVLAH
                     AAYRLLGLRTFFTTGEDECRAWTIKAGDKASTAAGVIHSDFEKGFIKAEVYHYNDLID
                     YGSETAVRAAGKMRQEGRDYVVQDGDIMFFKFNV"
     gene            28181..29428
                     /locus_tag="PLQ30_01270"
     CDS             28181..29428
                     /locus_tag="PLQ30_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPW01105.1"
                     /translation="MHDLSVYLSANAHNMKKSVIRELLKLTNQPDIISFAGGLPAPET
                     FPVEDLREASDQVFRKYGDKILQYGTTEGDNDLKARIIEYESSHGVNLDPDNILITSA
                     SQQALDMVPKLFLDPGEYVIAERPTYLGAIQAIQSYQGKVLGIPFSHANDGLDMDELE
                     KEYARAKAENKRIKYIYVIPDFQNPTGVCWSLEKRKALIEFAYREELFIVEDAPYREL
                     RFMGESIPSLYQLELEMQNLGIVVGLKTFSKILAPGTRIGWILARKDIIQKLVVAKQA
                     VDLCTSPLTQKIVAQFLATGKLEEVLKNTCAIYRDKRNFMLQKLETYMPKRPDLTWTK
                     PEGGLFLWLSLPSYVDTDKMFYKAIEKKIAYVVGSSFYYDDPEVNSMRINFSYSSYEQ
                     IEEGVKRLADTFKEEIEEHEGHL"
     gene            29682..30419
                     /locus_tag="PLQ30_01275"
     CDS             29682..30419
                     /locus_tag="PLQ30_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW01106.1"
                     /translation="MPIIQVIDVKKDYYLGNVVVPALRGISFAIERGDFVSIAGPSGS
                     GKSTILNIIGLIDIPTSGELIINEKNTKGLSDRELTRFRHEVLGFIFQSFNLIPVLNV
                     YENIEFPLLLGKTKIPKSEWKSWIDFLIDEVGLSDWRNHKPNELSGGQRQRVAIARAL
                     VTKPSIVLADEPTANLDSTTGEQIIDLMKKINRELETTFIFSTHDAKIVNIADHVIRL
                     RDGMIIGNKRNGEDILEENASIASAEN"
     gene            30422..31924
                     /locus_tag="PLQ30_01280"
     CDS             30422..31924
                     /locus_tag="PLQ30_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPW01107.1"
                     /translation="MPIIFKIAFRNIKAHRSKSLIIGVLLAMGAMVLVLGTAFINATE
                     EGVRSTFSDVYTGDIFISGISSDGPVSLFGVTSTSGMATTPIIPEYDKVISIVKSTPH
                     LEKTSSLATGFSQVIRENGDQETEEDSQKKIKPQDRFLFLFGIDAASYWDLFETVEIV
                     AGEKLKPGQSGIMINESQFHKFSDWFEKPLSIGDPLLIQGFSTIGFRLRELPIVGIFR
                     LKEQGTAPEQLAYIDIESLRVMSGMTVGANEPIQLKPEQTSMLSIEDPDELFSDDIIV
                     TSETSKHFDVASISRELSRPSEIDPLKPDEGAWQFIVGKADSSQNIDRIISSLNKAFE
                     KEEIQAIAGNWQVAAGPYGQSVDIVRIIFAVAIVILSIVAIIIIMNTFVISVIERTSE
                     IGTMRAIGAGKGFVRTMFAAEALALALVSASIGAGLGLLIIEILKSLRIEATNPFFLV
                     LFGGQYLRPIATAGNVIFAIILMIVVGFIAHLYPVSLALKIQPVRAMQEE"
     gene            31935..33260
                     /locus_tag="PLQ30_01285"
     CDS             31935..33260
                     /locus_tag="PLQ30_01285"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPW01108.1"
                     /translation="MNLVLLRIAARNLMRQRKRTMLLAGAIAFGIMIVTILNGFAGSF
                     VKNISDNFSYLMGGHVFVSGTEVTPSGKRISIIRDDSVIMQALEDSGQKWVSLQKTSQ
                     TIATLIFEGKSIQQTLTGIDLTHSGMIKERLALVDGNWENLSWSNAIIISQSIAKKLN
                     VLPGDVLVAQLQTITGQNNVGEFRVAAITIDSSFVGSIMTYVQLEYLNSLIGLGKNEY
                     MSLGIMLEKLEKSGAFANALYTSMKGMGLNLFDRSTKDENGSMTPFQAMLLEQRTEQW
                     TGTKYRLYTIDDVLAQAKQIVVAIDTGSTVILIVLFAIVMIGISNTFRMAMYERIREI
                     GTMRAIGVQRNEIRSMFLYEALLLSLAGAIAGIVLALIAMGLLTLISFDPLSPVFLIL
                     KRGHFSFYVPPLRAIGNILLIAVLTLVAVYAPANAAAKLPPAEALRTVK"
     gene            33277..34074
                     /locus_tag="PLQ30_01290"
     CDS             33277..34074
                     /locus_tag="PLQ30_01290"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPW01109.1"
                     /translation="MYSNVSRKAKSLRRGILSILSLLLLFFTGLNPLLAQSKPDPREL
                     IKQVDDLSDFSGKDFSAVFTIVTQKPGEKDSVTQARVFRRDAKKQFVILILLPEVNKG
                     QGYLREDDNVWFYDPTSRKFSHSSMKENIQNTKAKNSDFTLSTFAEDYTVTKMTESMV
                     GKFPVWVLDLKARTNEVAYDRVVLYIRKDRTMLLKREDYSVNGRLMRTTAYPKYVELE
                     GKLLPSQILIVDEVNKGEKSQMTMTEQSVSPLPDRVFTKAFLEQVSR"
     gene            34104..>34974
                     /locus_tag="PLQ30_01295"
     CDS             34104..>34974
                     /locus_tag="PLQ30_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01110.1"
                     /translation="MSLLRNLGAIGILCVIIMAAPFSISSQEESNDIDALFEDEIIVQ
                     QDSDTLGQEESQDSFIGQTNPLQSFLKTETVKIGGNIGGTVSASWKWNDPWTVGFDLA
                     NPDSHELAPALNSLLYFDARPEEDFRVHGSFKTAWPFAETKSLLDSKNKTTLVSVPDV
                     RIFELFSDFQLGDNAYLRFGKATVKWGVGYFFSPADIINLESINILDPTVQREGPLQF
                     RVFVPYGPSQNTLSFYSIFDSNKWDFDTTALAAKAEFLLGNYELSLNSYYRYDTTERA
                     ALTLTGPLGNFDIF"
BASE COUNT         9528 a         8199 c         7683 g         9564 t
ORIGIN      
        1 ttcttcgcac agcaagaaga agattttgct cgcgccattt tggaggggca agctccctct
       61 gtaagcggct ccgatggtct ccaggtggcg cgcatcattg aaggaattta cagatcgaat
      121 agagaaggcg taccgattcg gaactgacaa ctcgctttca gggatttatt aggcactttc
      181 tatactctat gtttgagtct tctcatactg gcgagcattt caatgtattc tcttaatttg
      241 aagtaatgag ctccaattca agccctgatc ggagatggat caagaattgg ttgatcaaaa
      301 aggccaaaca gcatctccat cgccaaactg tcgatactcg attgcttctt ctattgcaga
      361 ctctgtaatt tcgttctctc catctatgtc ggctatggta cgtgcgagtc taagaatgct
      421 atgcccagcg cgtgccgaaa ggctataatt agccatccct gatagaaatg ctttttcagc
      481 cgatcctgct atatgacaaa tatcatttac aatccctgca gggatatgag cattgttaca
      541 agtgagaatt tgatttgctt ccgaaaacat catgcttctt gaagacccca tgttttcctt
      601 ttctttctta ctcatcccat cgtttgtcct tttaaaccgc atgttctgga tttcgcgcgc
      661 agattttact ttatctcgaa gcaaatctac atctaccgct cgagattcga taaggcgaga
      721 agctttcgga ggctggattg cgatgcgcat gtcgattcta tccaggagag gtcctccgag
      781 ctttttccaa tatcgcctaa tctcttcggg agaacatatg caggtttttc cgcgcatacc
      841 caaattgcca caaggacaag gatttgcagc cattatgagc tgaaaatctg aaggaaagcg
      901 aagcactcgg ccggctcgca caatatcgac ataaccacgc tcaacaggct ctcggagtcc
      961 ctgcaagaca tcccgtctaa actctgggca ttcatcgaga aaaagcacac catgatgagc
     1021 cagactgacc tctcccggcc tcaggggttt cgcgcctccg agtattcctt ccatgctggc
     1081 ggagtggtga ggagctctaa atggcggcct ccttcccgag caggtccaaa ggctgcccca
     1141 aagagaatag atggaagcgg tatcgagagc ttcttgctcc gaaagatctg gaagaatcga
     1201 aggaaagcga cgggcagcca tggtcttccc cgcaccaggc ggtccagcga gaaacatatt
     1261 atgccctcct gcggccgcaa tgacgagcgc acgcatcaat ctctcatcac cgcgaaaatc
     1321 gctatatgtt tccctgcttt cattcagatc gagccacaag gagggagacc ttggcgggat
     1381 ttgcttttcc tgtccaattg gcaaatctcc ttgtgaaaat tgcccttctt gcacatctcc
     1441 ttgtggcgtt tttccctttc caatttctat aaggattcta tttgattcag ctagatgacg
     1501 aataggccac acctgaacgg ccttcaaaat ccgtgcttct tgggcattct caaaggggag
     1561 gatacaaatt cgaataccat gagaagcagc agagcgaaca gcgggcaaga ttcctcttac
     1621 gggccgaatt tctccttcca gcgtcaattc tcccattgcc atcgcacata tgggcaagtc
     1681 ccctatctga ccagattttt cgagtatttt taaggcaatg ggtaaatcat aggctgagcc
     1741 ctctttgggc aaatcggcag gagagagatt tacaagaata cgatcctgag ggaattcgaa
     1801 gcctgcattc ctaactgcga ttctagcgcg ctcgcgtgct tcgcgcacag atgtcgatgc
     1861 gagaccaact atttcgacgg cgggtatgcc gtttctaatg tcagcctcga tatgaactaa
     1921 gattccatcg aatccaagcg gttcatgtgc atagagatcc atgccgttcc ttagcctctc
     1981 ttcagtctct ctcttagaga cgcagagaga acggcattgt gcttcaattc tcttagccga
     2041 tcagaagaga atctaagtcc agctcagctg cggcacaagg catcattaca tcgaatgctt
     2101 ccagcaccgc tggatgaatc tcgccaaaga ctccgacact ttccttctcc aaaaagactt
     2161 caacctgtct gcccgggacg aagcgcggat cagcggactc acgcaccgtg tagttcttcc
     2221 ctaagaaata cataagtgtc gccacatgag aagcgatttc attgtaatct gcttgcgcat
     2281 gacttatcaa aaagccgatg tgctggcgag taacaacacc ataattctct tcagggtcct
     2341 ttatggcgac tttgcctact tcgaatgtcc gatgaggata tgaggctttg gccgatacac
     2401 tttcagtctg caagagcccc ggaattggcg aattcctgac atactcatag ttttcggtca
     2461 taggattggc tattttcaca agctgaccaa cgggtatttg catcttttca gcatagtcta
     2521 tacctgatcc aagataatta tagatcatct cctgatagcc aagacctatc attatatcct
     2581 ttgctttgcg cgagaatact tcgatgggag taagtcttcc tattgtaaaa tctgaaggtc
     2641 gctcgggagc gaaattctca ataccaaggc ctatcatgat atcttctgcg acatctacgg
     2701 gatggagaaa atcatttcga tattcgggtg gagttacatg caaggtgagc ccatcgctac
     2761 aataagcgcc aagccccatc cgagctaagg catcttgcgc ttttttcaaa tcgatagagt
     2821 ctccaagcag tcgatttgct tcgataagag aaagagttac tttgttctga aaataatagg
     2881 gaaagatcac ggaggatcca aatggactgt cgtattcata atcgacccgc acatttgcta
     2941 tttcaaagcc aatgtcataa aaatcgcaag ccattatgga agccgaaagc gctaccgaag
     3001 ggtaatcggt acctgttact tcaatgaaga cctcggagtc gtcaacctgg actgcaccga
     3061 tatcagcgct attgattatt ggcggatacg atagcacact ccccttagaa tctgtaagca
     3121 aaggatgtat ggttttgccc tctagaatgg atccatactc gacacccttt ggatgttctt
     3181 tgagaatctg acttaaggtc attgatcgag tctcctgaag cggcacaaag gacaccgaat
     3241 ctggctctac tccaaaatat cgaacaggcc attcgataag ccctgttcga tagattccta
     3301 tcgaaacccc ttttcgtttt cggccataat tccaagccag cttttcctga gtctgaatca
     3361 tgtcctttag cagagcgtcg cttataggct tgccctttgc aacgaaacaa gcaatccatg
     3421 gacggacttc tctcacggat ttttcgacaa caacccgata ggctgcatcg atactttttc
     3481 ccttagatgc aaagaaagga taagaaggaa tattccttgt tcggtatatt cgcaactgcc
     3541 gagccaatcc ggcagttgtc caaagatccg ggcggtttgt gtcatttagc tcaattttga
     3601 tcatgcgctc gcccgcatcc gaaggaatcc tatcccattc atccaattct gcctttgcta
     3661 tagtgaggtc ctgttcaaga tcttcataat tgctccagct ttgccctgca agttgaaaaa
     3721 agaactcttc gttgacttcg atttttggca tggtttatct ccggacctca ttgtattttg
     3781 tattgggatc gacgcatcct cacgctctcc agatcgctgc agaacaaatc tcgcagatca
     3841 ttgaggccaa gcgccataag cgccatccga tcgatgccga taccccaggc tagaaccggt
     3901 acttcgacgc caagagagct agatacctct ggtctaaaaa tacctgaacc gcccagttca
     3961 aaccatccga gcacaggatg ctttatatga acctcgatcg aaggctcggt aaaaggaaaa
     4021 tacccaggca catattttac ttccttggct ccagcaacct cgacagcaaa catctccaaa
     4081 aatcctagta gggtcttaag attcacattt ctgccaagca caatgccttc ggtttgatag
     4141 aaatccgaca ggtgagtggc atctacgcga tcgtatctaa aacagcgcac aataccaaaa
     4201 tacttgcctg gaacctcagc ctttggaagc tgccgagcgg ataacaccgt cccctgactt
     4261 cgaagtatga gccgccttgt aaaatcccga tcgaattgat attgccagcc acggctgccc
     4321 gtattgcctc cattttcgtg cgccgcagcg acattagata ggtatggctc ctcgatgaac
     4381 tttgcgtgtg tcggctcttc gatgtaatag acatcatgaa tatcgcgggc tgaatggaac
     4441 tgtggcatga aaagtgcatc cgagttccag aactcggttt ccacaaggtt accatcgaat
     4501 tccacgaatc ctagcgaaac tagcttgtcc ttgacgcctt ccaagaattc gacataggcg
     4561 ttccggcggc ctggtatgac tctggctggt ggaatctgaa cattgtaagg tcggaaatgg
     4621 cctttcttcc atgtaccttt ctcgagcatc tccggagtaa tagcgcctaa ttcctcccca
     4681 gtcataccca gggtacttat ggctttgcgg tattctttcg caataggagt gacacgataa
     4741 aggactctga ctctatcggt tattctgaaa gggctgtcct gcgcgccgcg cttctttgca
     4801 atttgcccca ttagcgctct ttgagaatcg gagagcgctt tttccgagac tgaaccgcca
     4861 ctggaggcag ccaacgcaag aagtgattcg atttccgaga gagacgcggg aaattcattt
     4921 tttgcgagcc tggcgcggcg cgcttcatct atcgcacata cggcttcttt ggagagcgaa
     4981 ccaaaggccg atcctacagt tttctggtca aaacccagag tttgagctat ttcgggcagg
     5041 ctcattggtc ctcgatcttt caatagagaa aatattctcc tggctggaat acccttctcg
     5101 tgccattctt gcccaagcgg agtgagctcg taaaagactt cggttcttct ctctgcttct
     5161 tcaagcaccc ctttggcaag aagccaggaa aaagcctgtt gctcttgacc ttctctaaat
     5221 ccgagaactt gtttgactcg ctgactatcg attatctcgc cctcgtcgaa tgctagaata
     5281 atcctgactt cgagcggatg cagtatccgg gccgcacttt gatcaatcat cttttcctgc
     5341 cttgttgtta gagtgaatat tgccggaaat gctcttgctg gtcaattgcc agtctctatc
     5401 gctcgcttca aattgcttga ttcggtagat ttgaaaatag cacccttgcc gctccgacca
     5461 tcaaagcgca tatcaagaga ggtatttaac tccgtaagaa cacaatgcct tgttcctacc
     5521 ctgggcttca atgaatagag ccattccaga tcgatatgat catccgctga accttgattt
     5581 atcttcatat attttatacg catggttgta agattatgaa aaaaatgtga ccccagtgag
     5641 ctgtccacta caaatcctgg cagatccatt tcgactataa cttttgcatg agaaatctga
     5701 tcaaaagata caggcactcc aagccatcta tccctcgtac cccatcttcc cggcccaatc
     5761 aacaaataat cgaaattctc cttccgtgcg aggacgtcga gttcttctat ctccgccgcg
     5821 atttccagag tctcatttct ctgaaatttg cgtggatcaa cccagactat gttatgaatg
     5881 ctgaggtctc tgccattacc catgcagttt cgagaaagca gaaaacatgc atccttctga
     5941 atagaactca aatcgatttc aatttgatct tcaagatgaa tgagcggctt cagctgtaga
     6001 agatacagag ttggttcctg cttgtcgtcg tcaaaattga gtgcatattc gatctccacg
     6061 ggtgtaccca tagaacgctt cccgatgtcg agcgctatgt ctattgcttt tgcaaatgga
     6121 tatgcttcat gtttcagtat attggccaaa tctattatgc gatgtccatc tgtcgaaact
     6181 cccggcacaa agcgctgatc cagtgaatcc caggtagaag cgaacatgga gaaatatcta
     6241 tcactctcag ccgcatctat cggcaattcg aatagactag ccatctctcc tttaagaaga
     6301 ttcggatctt tgtctctaag cgaaagaaca tgaaaatagc gctgtgtatt ctccgatata
     6361 tactctagcg gtcctatatc gagctttgga tatctggggc agaaacgaaa tgccgttcct
     6421 cctgtaacta gatatgttcc caacccaaga gccgcgatac agagtccgtc gtcaggcttt
     6481 acataagaga tcgggtaata attgtatgat tgagcaactc cagatatatg tggataaaaa
     6541 taatctccat gtcggttgcc cgccagttct tgtataatta ccgccattcg ctcttcttcg
     6601 agattatagt tagtagcctc gaaataggcg cgagcttcct tggaaaaaag gctcgcgtag
     6661 atcaacttta ccgctgttga tagctgctta aagcgtactt cgctgtcggg atgattgttt
     6721 ggtaatatat atgtatcgta tacaccagaa aatggcacca tgagcatgtc ttcgaaaaga
     6781 ccgctcgaac gcacagccag tggcctcttg ctaagcgata gcaactgcct cagattattc
     6841 tcaagtttct gagaaagggg cttttgaaca aagaccgagc ggagatcctg aacctccttg
     6901 tcgtagtaag cccaagacca taggccattc gattcaagaa agtgctcgaa ttcatcgata
     6961 cctataaaca cggtattcgg cattgagatc tctattcctg gcacatactg cggaaaatcg
     7021 aaattctcta agagcgaacg aatgaataga agtcccctgc ccttgccgcc cacagaaccg
     7081 tccgctattt tacataaata cctggagtgc ttgaccatct caggatcgag ttcaggcact
     7141 atccctctgg aatgaaccga tcgaatctgg tcgatcatgt tcacaatgaa ctgccgtaat
     7201 tcttccggtg aaccgaaatc agtgattctg tatggcttca tgatctttgc tacatttatt
     7261 tctccgcgtg ccataagcca agctgagaaa tgattatgtc ccgcatggta aagcaggctt
     7321 tctatgggaa tttcattcaa tttctggatg aattcgctca tattacctgc ttgtccgatc
     7381 tcggctccat tgggcatgcg aaagacgaaa ttcccaaagc ctagatttgc ctcaaagaaa
     7441 gcggcaagtt ccatctccaa ggattccgaa ttcttgtcga taaaactggc accaaaagca
     7501 taagcttttt cgcgcacatt tgtttcgcta gattgcacaa gcattggcag gtccggcaac
     7561 tcctgcttca caaatttaag aaaatcgaaa cctgcattat catcgaattc gccgtttctt
     7621 cggaagcgaa tatcggtgat caccgtaaga agataaggct tatagctttc aaatacctca
     7681 agcgcgtctt cataggtatt agcaagaagc accttcggtc tggctcttat actcatcagt
     7741 ttattagtct ccacaatccg ctctccttcg ataagggctt gagtctgttt aagtacaact
     7801 ctatataaga caggtaaata gcgagagtaa tatctgaccg aatcttctat aaggagaatt
     7861 actcgtactt gtccagcgtg agtgtcagcc ctggcatttc gccaatcttc gatatatttg
     7921 atcattccca caaagagctt ggaataacca ttccagacaa agattctatt gaagactgca
     7981 agttcggggc ggctcatatc cagcattgct aaactagaat tgtccgttac catgagaaga
     8041 atgggaatat ctggccaagt actcttcatt gagactgcaa gtcttagtgg catattaaat
     8101 tccgtgcttg ccattacgac taccaaatcg aaccggccat ctctaaaaag atccatagcg
     8161 gattcttcgg tataagcaca ggtgattctt ggaaccgcat tcagattgag tttgaaatat
     8221 tctccatata tttgctctgt aagcactcca tcagattcaa caacaaaaga atcatagagg
     8281 ctccctacca atagaatttc tcgtacgcgg tcgcgcatta gatcatgata gatattgcgc
     8341 tcacgccgct gactgtcata ggtcgaaacg agttcattga aatacatggc tcaattttat
     8401 tcatattttg cattaaaatg aagattagca aaccttgacc ctgtttctat gcatagctat
     8461 gattgccgaa acccctcaga ggtttttcga tagataggga ttgaatataa ggagatcgcc
     8521 gtgttcaaga atataaaact agcagcccgc aatcctgggc agaagactac ggtcatcgat
     8581 ataagcggcg taaaagtcgg tgatgacctt gttgtcattg ctggaccatg ctcggtggaa
     8641 acagagaagc agaccattga aaccgctatt gcggtaaaaa atgctggagc aaagatgctg
     8701 cgcggaggag ctttcaagcc tcgaacttct ccatatgcct tccagggtct tggattaaaa
     8761 ggccttaaga tcctcgataa agcacgccgt gagacgggac tgcccatagt cactgaggtt
     8821 gtcgacacgc gcgatgtctc ttgggttgga gagtatgctg atgtactgca aattggtgca
     8881 cgaaatatgc agaatttttc gcttcttcgg gaggtaggca agtctggaaa accaatcctg
     8941 cttaagcgtg gcatgtattc cactttggaa gaatggctca attgcgctga atacatactc
     9001 gcagaaggca atcccaatgt gattctatgt gagcggggaa ttcggacttt tgaggcttat
     9061 acgcgaaata cactcgattt atctatgatt ccggctgttc accgagaaag tcatttgcca
     9121 atcttcgtcg atccctccca tggaacgggc attctatccc ttattgagcc gatgagtctg
     9181 gcggcggtag cggcaggagt agatgggctg gaaattgagg tccatataga tcctaaatcc
     9241 gcgctaagcg ataaagatca gcaactcacc actgtaatgt ttgagtctct catgaaaaag
     9301 cttcgagggc tcaagcactt cttggatgaa tcgaatgttg cgtctgatga gtcgtctgat
     9361 gagtatgctt ccgaagagtg tatttcatct tcacatgatg ggacaaaagc gtcgaattct
     9421 gttggcacat tgaccgatgc cttctctgaa gccgtagatt aggaaaaacc taatgcaaag
     9481 aaatcaaagc ataatacgat ttggaaagac ctctgagttg ctctcgctaa ttcctataga
     9541 aaaagccatc tttgttacaa gcgagactct ttatggacag gcattggaaa tgctgtctag
     9601 ctcaagttta gatgcaaaag aagggctctg ttggatcaaa gtccccgatg gcgaagcctg
     9661 caaaaatttt tcggttttgg agactgtgtg tcgccaaatg atagatttgg atgctaatcc
     9721 agatacagtg cttgtggcca ttgggggtgg ttctctaagc gatctggttg ggcttgcggc
     9781 tcatatatgg atgcgaggaa ttgatatcgc gcttgttccg accaccttgc ttgcaatgat
     9841 cgatgcatct atcggaggga aaaatgccat cgatataggt cctacaaaga atcttattgg
     9901 cagctttcat attcctagac tgattctttg tgatgtcggc tggctggaaa ccctgccttc
     9961 aagagacttg ttatcgggca tggcagaggc tattaagcat gctgtcatcg atagtgaaga
    10021 gcatttccgc tttctagaag gaattgcgaa ttcggaagaa tcgctcaccg aaataaatgc
    10081 cgagctttgg gaggagctcg ttcagcgatc tcagcaggtc aagatccggt atgtagatgc
    10141 cgatccctat gataactatc ttaggcatgc tttgaattat ggccatactt ttgggcacgc
    10201 aattgagctt attacagaga ttcctcatgg atttgcggtg gcaactggga ttggtatggc
    10261 aaacgatctt gcggtatctc gcggcgctct ggatccggat atcgctgccc gcataaattc
    10321 gcttttgcat agtattggtc tgccggcgaa tgttgcggaa gtttctgcga ttgctggctg
    10381 caatattcgt ccagcggcaa tatacgatct tatacgggct gacaaaaaaa ggcggggaga
    10441 gagcatcgat ctcgcactgc tacgaaatat tggcgatatc cgaatcgagc ctgttgcact
    10501 acaatatata gcggaattct tgggagctta ggcatttctg gagttaataa ggggaatcat
    10561 gaacacgata ggcaaaatat tctgtatttc tctctatggt gagtctcatg gtaaagccgt
    10621 tggagttgta atcgatggct gcccagccgg tatttcgcta accgaagaag attttgcaga
    10681 agatctgaat cgaaggaagg caggggcgga aggaacaacg acaagaacgg aatcggataa
    10741 gccgattctt ctatcgggag tctttgacgg tcatacaaat ggcgcgccta ttcatatcgc
    10801 attccgaaat gagaatatcc gatcctcaga ctatgagcaa tttatagata ttccgcgacc
    10861 aggtcatgct gatttcagcg caatgatgaa attccaggga tggcaagatc caagaggatc
    10921 cgggcatttc tcagggcgtg tatccattgg ccttgtcgcc gcagccgttg tcgcaaagaa
    10981 aatcctcaac agcttttccg atattgtttt tcggacaagg ctgctcgaag ccggtggatc
    11041 tagagatata gacaaagcaa tttatcgagc aaaagaatct ggagattcga taggcgctct
    11101 tgtggagatt agagtatccg gagtaccggc cggatgggga gagccttttt tcgatactgc
    11161 cgagagcata atcagccatt atatttttgc agtgccggcc gtacgtggcg tggaatttgg
    11221 cgatggattt gctgctgcaa aaatgcgcgg ctcccagcac aatgatccat tcatagactt
    11281 gcatggaaga acagcacgca atggagcggg cggtatcaat ggcggtatta ccaatggaaa
    11341 tgatattttg ttaagagttg cggtaaaacc agcatcatcc attgcctcta cgcagaacac
    11401 gcttgatttt tctcgcgatg agatggttaa tcttgaaatt catggacggc atgatgcctg
    11461 catcgctctt cgaggcgcgg tagtcttgga agcagccacg gcctgtgctc tggccgatct
    11521 cgctcttgtg gcacgcgcag aagctccgtt tatatccaat atcccatgga ggaaatcatg
    11581 aatctcgatg atattcgaag tgacatcgat cgcatagatg ccaaaatact ctcgctccta
    11641 aatgagcgaa tggaaaaatc acttattgcg aggcacttca aggcagtagc cctggatccg
    11701 gtgcgcgagc agattgtcct tgaaaaggtt cgccgctcga gtcaatgcct cttggatccg
    11761 catttctcgg ttcatctcta tgagcagata atatcggaat gccgacacct ccaggatgcc
    11821 ggccttaaga ccgtagcgtt tcaaggcgaa cacggagcgt attcggaagt tgccctgcga
    11881 ttgcttatgc caacagcggc gaccattccg tgttgtgaat tctcggatgt cttcgatggt
    11941 gtcgagaagg gtatctacga ctatggcatc gttcctgttg agaatacgct tggaggcatt
    12001 gtagggccgg taaattcgat tcttatatat acacgcttaa aaattgttgc agctatcgac
    12061 ttgccaattc gacactgtct gctcacaatt cccggagccg atcatcgtga attgagaata
    12121 gctcgatctc atacgcaagc tttaacacaa tgccgcaatt ttttgctaag gaaccatctg
    12181 gacccagagc ctttttttga tacggcgggc gcggcaaaag cccttgcgga ggaaagaccc
    12241 aagggtgtat ccgttgtggc aagcaaattt gcagcagatc tctatggcct ggaaataatc
    12301 aaagaggata ttcaagattc tccgcataat cgaacgcgct tctttattat cgcgacagag
    12361 gaaaacgggg ttgagggcga caagtgttcc gctgttttta cggctggaaa caaggccggt
    12421 gctctattct cagtgctcga ggtattcgct gaagaaaata taaacctaac gcgaatagaa
    12481 tcagtgcccg atgtgccgga aaaatatgcg atattcatag actttgaggg ttcactctct
    12541 tcagctccag tgcaacgggc gctggaaaaa gttccggaga ttgccgaaga ttttaaaatc
    12601 ctgggatgct atcatgaaat gagggtagaa tcatgagaat cgcaatactc ggcgcaggcc
    12661 gaatgggcgc gtggctcacg gaggagctct gttggaataa tgaagtcatg gttcatgaca
    12721 gcgatctctt gaagatgaag tattttatca aggtacaccg agcgcttgcc atagaggagt
    12781 ttgccgattt caagcccgag ctctttataa actgtgtacc tcttggcttg acacttgatt
    12841 cctttgacgc agttcttcca tatttgcctt ctcaatgcat cattagcgat atagcaagcg
    12901 taaaaacagg ctttcgagaa tactacgaaa attgcagaca tccatttgtt tcgagccacc
    12961 ccatgttcgg accaaccctt gcaaatatca gagaccttca tgaagaaagt gcaatcatta
    13021 tttcggaatc aagcaaggag ggcgcggatt tctttcgtcg attctattca agcctaagaa
    13081 tttgcatttt tgaatatagc tttgaagaac atgacaagac tgtcgcctat tcgcttgcta
    13141 ctccttttgc ttctacaatg gtatttgcag cctgcatgaa aaagcaagat gcgcctggaa
    13201 cgaactttaa acgacacctg gcaattgcaa agggcttgct atcggaagat gatcgtcttt
    13261 tgacagagat tatgttcaat ccctatacca tacgccaact ggagcttata aactctaaat
    13321 tagcttatct gacccatata attcgacagc gagattatga tgaaatgaag aaattccttg
    13381 ataagctgcg cgctaatata gaacaataga caagaatctc tgaatcgccg ctaacccttc
    13441 acatggatcg aatcgatatt tattggactc ctaagctctt ttggcacttc agccttaaac
    13501 tagcgaagtg ataatcaaga aaccactata gaagaaagat catcaaaaaa gcccggccag
    13561 gacttggata cacactctgc cccttcgatg gatacttcgc cacttgctac aagagctgcg
    13621 atggaagctg ccatagcaat acgatgatct ccatatgatt caacagtagc tccatgaata
    13681 gatccaccta ctatgtacat cgcatcgttc tgcaatgtta catctacacc tagcttccga
    13741 aatgtcttgc acaatgctat tgagcgattg ctttctttgg ataaaagccg ctttgtccct
    13801 ctgatacagg acctgccctg aatggcagct gccatggcta cgaggggagg aaaaaggtcc
    13861 ggacaatttg ttgcatcgaa atcaaaggga gcaagggcgc ttgcccttac aatacaatcc
    13921 gagcctttga attgcacttg agcccccgct tcagttagaa catccacaat ggcgcgatca
    13981 ggctgtgttg agtcttgatg caaaccatgg atctcaaggc cttcttcact tcctgctatt
    14041 gcagcgccca cagccaggaa agcaccggca ctccaatcgc cctcaataat caaatcagta
    14101 gccttataga cctgattccc cggtatccaa aagccggcac cattcgcatt tctttttatt
    14161 tcgaccccaa attcggcaca tatttcaata gtgagatcga gatatcccga gcttacaaga
    14221 ttatctacaa ttagctctga atcaccatcc aaaagaggca aagccataag aaggccggta
    14281 atgaactgag agctccctag agcatcgatc ctgctcttca agggatgaag aggaccttgg
    14341 attcgtatag gctgaactcc gcctctcgat atacaagaga tgccaaggct ttttagagaa
    14401 tcctcgacca tattcatagg tcgactagaa agagatgctt ctcccctaag ctctacctct
    14461 ctgtccaaca gagcaacaat gggagagaac attctcatgc aaagccctga ttctccacat
    14521 ggcaggacta ggggtaagca tggttgaaat ccattgtttt tcaggcctgg atagcatact
    14581 ttttgcgatg tagtggccaa tttgcctttg gcatctttat gaaacagggg cgatcctttg
    14641 atttcgagaa tccctttttc ttccttaacc tttgctccca gctcttttgc aatgataatt
    14701 gcagcgcgcg catcgctgca tacaggactt gcctctatat ggcttattcc tcgtgctaga
    14761 atggcacaag cgatcgcgcg ctgaagggca ctcttcgatg ccggggcttg tatgctaccg
    14821 caaaccttcc ccggtttcgc gaccctggtt cccatagaat ctatacaaaa acctctacaa
    14881 ctacacgagt ccttgcgcaa tcatagcatc agcaactttc ttgaatccgg caatgtttgc
    14941 gcctactaca tagttgccct cgaagccata ttttgcagcg gtttcaatac aactcttatg
    15001 gatgtttgtc attatttcgt gcaatcgtcg gtcaacttcc tcggcgctcc aggacatacg
    15061 catcgaattc tggctcattt caagcccgct tgtcgctaca ccgcccgcat ttgccgcttt
    15121 gccggggcca aaaggaatct tcttctctat gaagtacttc caagcttctg gcttacaacc
    15181 catattcgag acttcgacaa cgaccttgac accattttcg actagcattc ttgcatcttc
    15241 gccactcatt tcattctgaa tagcacaagg catcgctagg tctaccttct gctcccacgg
    15301 tttcttgccc tcgtggaaca cggcgttggg gaattttctg acatagctct caaccttgtt
    15361 gcggtctatt atgagcatct cgttgagata gttccacttt tcttttgtcc ctactccatc
    15421 cggatcatag atatagccat ccggcccgga aatggtaacc accttagcgc cgcgctggct
    15481 agctttcatg catgcgcccc atgcaacatt gccaaaccca gatatggcca ccgtcttgcc
    15541 ttccaatgtg tcacccatgg tcttgagcat ttcttcagcg aaatataccg caccgaagcc
    15601 agtggcttcc ggcctgacaa gcgaacctcc ataagtcaag cccttgcctg taaagacacc
    15661 cgtgaactca ttcgcgattc tcttatattg gccaaacatg tagccaacct cacgggcacc
    15721 tactccgatg tcgcccgcag gcacatccgt atctgcgcct atatagcgga atagttcggt
    15781 aatgaagctc tggcagaagc gcatgatctc attgtcgctc ttgccattcg gatcgaaatc
    15841 cgaaccgccc ttgcctcctc ccataggaag accggtgagc gaattcttga atacctgctc
    15901 aaatccaagg aacttgagag tggagagagt gactttgggg tggaatctaa taccaccctt
    15961 ataaggccca atggcggagt tgaactgaac gcgatagcct cggttgacat gatattcgcc
    16021 cttgtcatct acccatggca cacggaatat cactactcgc tcgggttcga cgatccgctc
    16081 aagaatcttt tgctggcgat attttggctc tgcttcgatg aggggagtaa tcgattcgag
    16141 gacttcctca gtagcttgca agaattcgct ctcccatgga tatcgagcgt gaagcccatc
    16201 cagcacttct tttatgtagg aattcatttc tccctccttt tagggattgt tgttaattga
    16261 tctgtggggt aaagatcact cttatgattc tataccccgt tatcgatttc cgcaagcaaa
    16321 attgggattt agggcaatgt ttcattttat gaaacatgca taggtgtaaa aggagaatcc
    16381 tcttggctca tgtcaagctt ttctatgcag atttttgtca acctgtggcc ttccatctct
    16441 tcaactgtaa attgcagatc accgactgta acctgctctc cctttgaagg tatccgctca
    16501 aaaagatcaa aaacatagcc cgccagacta tcatattcct cataaggcaa ctcggcgcct
    16561 attgcatcat tgaggtcttc tagatgaatg cgggcatcgc aggaccagcg attctttccg
    16621 attggcacaa cctgatctgc ctcttcgtcg aactcatcct gaatttctcc tactatctct
    16681 tcgattatgt cttcaaggct tattatgcct gcagtccctc catattcatc cactgcgata
    16741 gctatgtgta catgacggcg tttgaattct ttcagaagag tatctattcg ctttgtctct
    16801 ggtacaaaat agggaaggcg catgagactc ttaacttcca gaggcttttt tttcacaagg
    16861 gcagctagaa catctttcgc atatagaata cctactatgt tatcaatggt acctgaatat
    16921 actggaattc gagaatgccc agaatctact aaagtctcga gaatttcatc gatgggacta
    16981 atatcttcga cgcacactgt atcggtacgt ggaaccatca catctctaac aatggtctcg
    17041 ggaagatgct ccacgccttc gatcatttct ctttgctctt cgacgacttc atcctttgat
    17101 tcaacggttt ttccgtgtac gccaaagagt tgtgaaagaa tattcataga ataacatttc
    17161 ctcccaatgt ctttaggagt tgttcctgaa gcagcagcat tggctgatcc ggcgaatcat
    17221 cttcgtggct cataccagtt aagtgtagaa tgccatgaat aattagacgg cgcaactctt
    17281 catcttctcc aactccatat tcctcggcat tgcgctcgat cgcaggaatg cttataacaa
    17341 tatcgcctgc gagatagata ctctcactgc cttctattgg ctcatactca ccaagaacga
    17401 aagagagtac atcggtagga gagtcgacat gtcgatagtc tctgttaagg gtttgtattg
    17461 tattatcatc gcagaatgtc aatgaaatct gccagtcttt tttcccaagt gcgtccaaga
    17521 cagaaagtgc atagtcggca gatctctgtg cccaagaggg ctcatcaatt ccaatcatcg
    17581 aataaaatac ttcgtttgtg ttcattgttt cctctgctcc ggatattcca ggcgtgtatg
    17641 catctgcgat tgaagaattc gcaaaaaggc cttttctgcg acctcaatat cattaaatgt
    17701 gagctcgcta tcagagagct ggccactggt gagtttctta ataatcaatt gatgtataag
    17761 atcttcaagc tttgccgcac ttggattctt gagagagcga gaggctgcct cgatactatc
    17821 ggcaagcatg actatccctg cttctttgct ttttggccgc tcgccttgat agctgtaaga
    17881 ttgcaaatcg aattgtgcca cgttattctt tgccttgact agaattgctt ccattacaga
    17941 gttgccgtgg tgctgcccta ttatttcgat gacttcctta ggcaggccaa tttctcgagc
    18001 tctctctatg ccatccttga catggcgtct tattatgctt gcagagagag ttggattgat
    18061 atcatcatgc agattgatgc cttgttgatt ctccgcaaaa tattccgggt gatcaatttt
    18121 tccgatgtcg tgatagtagg ctcctatgcg cgctaaaagc ggatttgcgc ctatgtcctt
    18181 agcagcggct tccgcgagat ttgccacatt catcgagtga gcataagtgc cgggagcctt
    18241 cattaacatc tctttcataa taggagcgtt tatatccgcc aattccatga gtctaaattg
    18301 cgttgaccga tcaagaaggc gctcaaagag aggtaaaagc aataagataa aggatccacc
    18361 tgcgaatccg ttgagtgccc ccagacccat agactgaaag acttcggctg cactcggttg
    18421 ttgattcaaa agcagtataa ttgccagaat cgcttgaaca agagcctgca agacagcggc
    18481 cctggcaagc ataatacgag tgtgtgctgt gcgcataatg agtgttgcag aaattccagc
    18541 caacagaatg ccgatcataa attgagcatt gagatttgtg gcggcacctg taagaaagaa
    18601 ggataccaga ctaaagaaaa tgcctgttcg tgctccatag agcaacgaaa caagaccggc
    18661 aaacaatgac gccgggacaa aaagagctcc atgagaagga cttttcccgt tcaataaatt
    18721 ctgaattacc agaatccaat agaaaagaat gagaaaagaa atcaacaaga atataagtga
    18781 ggagcgatcc acaggaaaat cggataagtc aggcattctc gacagaataa agcctaacaa
    18841 agacgctatc aaaaggagcc cgagtcctgt aagaagtaca ccgatgtccg aaacaaggat
    18901 agaagaacga atagtcttta tctgctgata gacctctggt gtgatgagct ctccttttcg
    18961 caccaggatt tcattcctgc ttacaaagcg agtcactggc tccacacgag cgcgcgccgc
    19021 agcaagcctc ttgtccgaga ggttttgatc aaaaaaagcg ttttctctta agagcgcctt
    19081 ccctaaaagc ctcacatatt gctttagaga ttcgggaata tgtctagcat ccatttcatc
    19141 ttccaggcga ttattaagat tggcaagtgt aatcatgttt ctcacggcga ttttttcact
    19201 ctcttcccta ccgttcaaca ttcttaccag ttcataataa tccggattga atcgatcgaa
    19261 gacaccttca ggcacggaga caatgccttt gctcaagaga ctctctacaa tatcgctcaa
    19321 atatacaaag gtttgagcat ttagctccgc gctgacaaga gtcttatatc tgtcggattg
    19381 caatagatca ggaaagcggc tgctaagcat caacgataat gcatcgatgc ttccagactc
    19441 actacccaga agctgcaaat aatatgaaga gaattcttca gccttttcgc gaatagatgc
    19501 ggtgatcttg tcatccatca caaagacagc aagcaccaat ctctcctcag cctgtatctt
    19561 tctctgtgtc gcctctttgt cgatataaat agtgtctttg ccagcataaa tatcggtctc
    19621 ggcaatctca cctactttta ttgccttaag cgagctacga cttgaaatcg atgatggtcc
    19681 gaccaggatc agaccaattg ccgctatggc aattgctaca aagaagaacc ataagggcgg
    19741 ttgtaccgag cgaagagatt ttttcagttc aaagaaccag gattctcgat tattcttttt
    19801 cataggcttc tataattttc ctggctagag agcttcgaac cacgtcgcta gcatcgagcc
    19861 tgcttatgaa tacttcatcg atatttccaa ggagagttat ggcatgaata agacctgaat
    19921 ctgtcttctt cgggagatcg atctgtgtga tatctcctgt aacgatcgct tttgtgtgct
    19981 ctccaagccg agtaaggaac atcttcattt gctcttttgt tgtgttctga ccttcgtcca
    20041 gaatgactat gcaattagcg aggcttctgc cacgcatata agcaagtggt gcaacttcta
    20101 ctgccctgct ctcttcgagt cgcctaatgg tctcgtatgg gaccaatgct tccatagcat
    20161 cgtagagggg gcggagatag ggatttatct tctgctcgag gtcacccgga agataaccaa
    20221 ggctttctcc cgcctctact actggtcgcg ttaaaatcag ttttctcttc gatcgcgtca
    20281 agagctcccg cagcgcatag gccatggcga gatatgtttt gccggtccct gctggcccta
    20341 tagaaaagat gaggtcgtgt gacgcaatgc ctttaagata aagactctgt cccgaagacc
    20401 gtggaaatac cttaccaaaa ccattcggta tctgtatgca gttttcgaga aacctggaaa
    20461 ttacgctctc attttcctca tcagcattat ctatcgtagc tttgaaggat tgacttccat
    20521 ccggtgtaag ctccgcatgc agagcaataa tgagctccgg agtagccggt acaccttcct
    20581 ttatcgaatc tccaattgcg ctgataatgc gtgagaaaag attgagtatt cccgggtcgt
    20641 cgctatccac aaaaagctca ttgccccttg ttagcacatg agtccccaat aggctcccaa
    20701 taacagatag attgtaatcg ttagcgccac aaagctcggc aaatacctcg gtatcttcga
    20761 gcacaatatg tgaaggactc aatcgcactc ctgtctaagt atcatattct ttaataatat
    20821 actaatacct ggaggctctt tccagccttc agatctcatc cgcacttttg gctgttgttt
    20881 cttttctctt gagcgttggt ctcttagatc ttgagcctct atgctcgacg aattcccaat
    20941 aaggctaggg cttatattca ttgcttgaat gcaccttctg agtaatttat cgaactcttt
    21001 attgcagaga tatccttcca cgtaaccgca aggttgaatg ccacttccat ctcgtaatgt
    21061 ggcaatggtt gagtcgaaga tgccgttacg agcgttggtt cagccagtat atttgcggat
    21121 aatacccaat catgagcatc caatgaaagc tctagcttta gaccctgcaa cttaaaaaga
    21181 gtatttttaa gcttgttagc atcccagata gacaccgaat cccaaaggtc agcaaagagg
    21241 tttttagaat agtcctcggg atcaaaatca tctgattttg gaaacagaga cgcataatat
    21301 cgccagaatg aagagttcac agaagacagc gtaaaaccta aagataaacc tgcagaattc
    21361 ttgatggaaa gaccaaggtt tgctccaaat gtagaattcg taaactgaat tagattctga
    21421 ttgagagtaa gtaatccatc tgcctcgaca taccagctca tcatctcagt agaaaatagc
    21481 ggtgaactgt aacgcatttt gggtttccaa gatagagata atgttgaagg cctgaagtac
    21541 tgagtcccat cctgaatcca tgaactgttt ttgaaaacat agccgtttgc tttttgtgcc
    21601 acaaagcgtg cgttgaatga ccaagccgac agattggcac tgaggcttaa gggagcttga
    21661 gtatcgaaat cccaggcagc attcgcagat atctcgattt tttttattgg cctaagagca
    21721 aggttaccca tcaatgatgt cgattttagg tcttccagtg agtttttgcg agaaatgaca
    21781 tagctggctc ccactgtcgc aaaaccaaga gccgatgaac ccgaaagagt gtagctctcg
    21841 agaataggcg gtagagtggc cttgaaactc aagcgttcgg tcgtggaccc aagctttgcg
    21901 gccagctccg caagtataga atgatcagat atcatatttg gatcccatga taaccaatac
    21961 aatttataaa taggataggc atctactccg ctgccatcga ggccttcgta ggccaatttg
    22021 gctaccttcc cttggataga ataagagact tttgaggcag aaaaggtatc ggatttgatg
    22081 aacggttccc atgacaactt ggtcccggtg ttccagttag ccgtattgta actgtagtcc
    22141 gcaattagaa aaggatgtac agttgtggca tttacgcgtt catcgtagag atagggtcta
    22201 tattgcagct gccctaatat accggtcgaa atctgaaagg tcaatccaac gctatctatc
    22261 gaataggtag agctcagatt agtgttgctg cggccaccgt ataaccaata ccagctttca
    22321 aagtcgatat cttgaggttt ctgccatttg gcattataga atcgatcttc caaaaaactg
    22381 gaattcgtat tagtccattt cagcgacatg tttttgctct gcagaatagt accagacata
    22441 gagaaagctg tatcaataat gcgcgcacta tcgggataaa agaagtatct ttgcggatct
    22501 accgcattga ggcgcttctc tgtaggattc ataccggctg ttgaagcata ttttcttttc
    22561 caattgaaag atgacgatag attggagagc gtaaaagcaa aactattttt ctcaataaaa
    22621 ggattccacg accacgaaag gcttgctttt tgtgaaaaag aactgcgttc atctattgtc
    22681 ttgagggaag aactgccaag gatagaaaaa aaatcatagg attcatttcg atccataaaa
    22741 tcctgttcaa cgaatggatc ggaataaaaa ggcaaactga tctgccactt tattcccgcc
    22801 ttattctttt tagaagaggc ttcgaatttc attagaatgc gcatcggaag gtctatcgat
    22861 gagagcttcc atctattcca gaccgaagca tagtcaccgg cactatcata aggagaatag
    22921 taacccgtcg attccagaaa aacagaacgc gaaaaggcag ctccaataag ccaagaaata
    22981 tcgtatggat aggtatttgc caattctcct cttagcccag caaaaacccc cagggctgaa
    23041 tagacatctc ccaaaatcgc tatatttgtc tgattcttgc tttcatcagc aggcttagtt
    23101 ttggaaatat aagaaccaaa tgagcctgat gttccttcgg tagtggatag cagggaagat
    23161 ttctgtactt cagaactctg tgctccctta agataggtgg ttgtctgcaa aaaagctcca
    23221 gcacgagatc taaaaccgac cactggatga aattccgatg ccttagaggg ataatagaac
    23281 ataggaagcc atatgactgg tatcgcaccc acatataata tagcgttcga tgctgcccaa
    23341 tctccagatt cgaacagcca gatctttttt gctcgcagaa tataatgagg ctttatagcg
    23401 tcgcaggcag taagagagcc attggctaat gcaatcacct cttcggatct ggaaatcaaa
    23461 gactcaaaat gtactatgag tttgcgggaa cctggggtgg tagactccat gtcgaaagaa
    23521 ccatcgataa agacaccaga gttatcatct agatctacgg ctatggattc tcctttgtag
    23581 atatctgttt tggattcggt ccgacgcgta tattctacat cgccataagc ttttacttct
    23641 tttgtatcac gattatagac gacatactga gcctttatcg aatgcgtcac tccgccggag
    23701 tcaattaacc ggattattag aggccctgta agacgaagct cttttgttcc gttttcgagg
    23761 ctaaaatact cggcacttgc agcttgctcg atggttaaag ttatatcgct atcggatttc
    23821 tgcacaacct cttcaggttt ataagctcta ttgtcatttt gaatctgagc ttgggcagca
    23881 caaggcacaa gcaagatccg aaatataaaa ataatgccaa ttatgcgccg catcgattcg
    23941 tatggtatat ggaaaaatgc ggggttggca agaataccat ttctctacta gctcatatgt
    24001 tcagcaatct gcgcaagtac tgacctgtat atgaatccct gcaaagggca atttcctcag
    24061 gagtcccttc cgcaattacc ataccgcctc tttcacctcc ttcgggtccg aggtcgataa
    24121 tccaatcggc ctgttttatg acatcgaggt tatgttcaat catgactacg gtatttccac
    24181 tgtctacaag tctacgaatt acctcgagca attggcgcac atcagcaaaa tgcagccctg
    24241 tagtaggttc atccagaaaa tagagcgtac gcccagtggc tcggcgtgcc agttcgagag
    24301 agagctttac tctttgtgct tctcccccgg aaagcgtcag ggcagattgt cccaggcgaa
    24361 tataaccaag ccccacatcg accatcgtct gaagtttatg cgcaatcgaa gggatatgag
    24421 caaagaatgt tcttgcttct tcaaccgtca tgttgagcac atcggctatg ttcttgccct
    24481 taaaacgcac atccagggta tcggaattaa atcgcttccc atggcagact tcacaggtga
    24541 tatatacatc tggcagaaaa ttcatctcga tcttgattgt gccatctccc tgacaatgct
    24601 cacagcgccc acctttgaca ttgaaactaa acctccctgg cttccagccc cttgctttcg
    24661 attcgggcaa gcttgcaaac agttccctga tgggtgtaaa tacaccaaca taggtggcgg
    24721 gattcgatct aggagtcctt cctatggggc tctgatcgat attgatgatt ttatctatgt
    24781 attctattcc ttcgaggctc ttataggatc cttcacggat cgcggagtgc atcacacggt
    24841 tggaaatagc cggatataat acatcagaca agatagtgct ctttcccgag cctgatacac
    24901 ccgtgataca cacaaacctt ccaagaggaa tctttacatc gatattcttg agattgtgct
    24961 cgctggcgcc cttgagggtg atgcaagcgc cattcccttt tcgccgttct ataggaactt
    25021 cgatcttgag agaacccgcg agatattgcc ctgtaatgct ctggggattg gcaacaacct
    25081 cttgcacgct gccttgagct actatatagc ctccgtgctc gcccgctccg ggtccaagat
    25141 ccacaatata gtccgctgtt cgaagcattt gttcatcatg ttctacaact atgagggtat
    25201 ttccaaggtc tctaagatat gtgagtgtat cgatgaggcg ctgattgtct ctctgatgaa
    25261 gaccaataga tggctcatca agaatataga gcacgcccac gagagaactg cctatctgtg
    25321 tggcaagcct gattcgctgg gcttcaccac ctgagagagt cgatgcctgg cgctcaagcg
    25381 tgaggtattc aagccccaca ttttgcaaaa aaccaaggcg tgcaataatc tccttgagaa
    25441 tcagcttaga aatttgagct tcagtatctg ttagtctgag agacttgaag aattggacag
    25501 aatcctctac gggcatgcat gaaatctcgt agatattctt gctcccaatg gtcacggcta
    25561 gagcttccgg cttcaatctt ttcccatgac atacctcaca ctctttttcg accatgaatg
    25621 attctagcca atcctttatg tcgttgcttg tcgattccat atagcggcga cggaggtccg
    25681 ccaaaattcc gggaaaccta gactcatact cgaaatatcc ggtcttatcc ctattgttgt
    25741 agcgaacatg tatattatca tcagaaccat agagaatagc attcatcaca taagaaggta
    25801 gcttatcgaa aggagtatcc aggctaaaat gataatgatc ggctagcgct tcgaatcgtg
    25861 agcggttcca tgccgaatta ggattatagg ctagacaacc tccttcgttg aatgacaagc
    25921 ttctatctgg aataaccaag ctgggatcaa actccatgtt catgcccaat ccactgcaag
    25981 ccggacaagc accttgtgga gcattgaaag agaaaagcct tggctccatc tctggcaaag
    26041 aaataccaca tatcggacaa gcgccttttt ggctaaaaaa atcttctttt ctttgaccat
    26101 ccatctcgcg cacgatgatc acaatgccat ttgctatgcc gagcgaagtc tcgaccgcct
    26161 cagcaactct gcggcggctc tcttctgata acaaaatgcg atccaccaca agctcgatcg
    26221 tatgctttct ctgcttttcg aggactggag gagattcgat atctatgaat tgcccatcga
    26281 tccgtgccct caagaagccc tgtctaagag catcctccag tattttttta tgctcgcctt
    26341 tctggcttcg tattaccgga gctaaaactt gtatctttgt gcccataggc catgagagaa
    26401 taacatcaag aatttggtcg acactttgct cgcgaatctc cctaccgcag gaaggacaat
    26461 gaggaacacc tattcttgca aatagaagtc tcagataatc atatacctca gtcaccgtcc
    26521 cgacagtcga tctgggattg cgagatgttg tcttttgctc gatcgaaatg gctggagaaa
    26581 gcccctcgat ataatcgaca tcaggtttgt ccatccgccc aagaaattgc cttgcgtatg
    26641 cagacagaga ttccacatag cgccgctggc cttccgcaaa aatcgtatcg aaagccagag
    26701 agctctttcc agaacccgac aagcccgaaa tgataattag cctatttctc gggagttcga
    26761 ggtcgatatt tttcagattg tgttcccgtg cacctttgat gatcagtttg tccattgttt
    26821 gaaaatcata ctgtggtccc tggcataaag acaagtatgc ttgacagatg tatagctatt
    26881 caggctctgc tagacattga acttgaagaa cattatgtct ccgtcttgaa ccacgtagtc
    26941 gcgcccctcc tgtcgcatct tgcctgctgc ccgtacagca gtctccgaac cataatctat
    27001 aagatcattg taatgataga cttcagcctt aataaagcct ttttcgaaat cgctatgaat
    27061 aacgcctgcc gccgtcgatg ccttatctcc agccttaatt gtccaagcgc gacattcgtc
    27121 ttctcctgtt gtgaaaaaag tgcgaagacc caatagtctg tatgccgcat gggcaagcac
    27181 atcaagccct ggctcctcga gccctatgct atgaagaaac tcagctcttt cttctgaatc
    27241 ctctatgtct gcgagctctg cttcgtattt gccgcagatt acaaccgcct ccgaaccctg
    27301 tttttctgca atcgatttta ctatatctac atatttgtta ttggccattc ccgattcatc
    27361 gacattgcag acgaataatt cctttttgag agtcaataga aagctgcgcc gagcgtaatc
    27421 tacctcttca ggctcgagaa tagccgtaga agccggtctt ccctcttcca agataggccg
    27481 cagcttttgc agcacggcaa tttccttttc gatatccttc ttgagattag ggtcttgaac
    27541 cttgacctgg cgactcaact tatccagcct tctatcgatg ctctccaaat cagccaatgc
    27601 aagttcggta tgaataatct cgatgtcatc ggctggatct atcttgcctg atatatgaac
    27661 cacattagga tcatcaaaac atcgtaccac gtgtgctatc atgccaactt cgcgaatatg
    27721 ggagaggaat tgattaccaa gaccctctcc cttcgaagct cctcgaacaa ggcctgcgat
    27781 atcgacaaat tcgaccaccg caggtacttt tctttttggt ttgaatatat caaagagcct
    27841 atccaagcgc ttatccggca gtggaacaat accgacattg ggatctatcg tgcagaaagg
    27901 ataattcgct gtgtccactt ttgcccttgt aagagcggaa aaaatggtgg atttgcccac
    27961 attgggcaaa cccacaatcc cacagttgag cggcattagg gcattcctct aattgctgta
    28021 ttgattacca aagtatagca aggacaaatt atacaacaag taccatgccc gccatctaga
    28081 aaaatgaaac tcaggaatat aattttgaaa acatatattt acgaatcata aaaactgaag
    28141 taggatgaaa caagaaccac aaatcaagga ggacttatca atgcatgacc tttctgtgta
    28201 tttgtccgca aatgcccata atatgaagaa atctgtaatc agagaattgc tcaagctcac
    28261 taatcaacca gatatcatct catttgctgg tgggcttcct gcgccggaga ccttccccgt
    28321 tgaagacctc agagaagcaa gcgatcaagt ctttagaaaa tacggtgaca agatcctcca
    28381 atatggcaca acggaaggcg ataatgatct caaagccagg attattgaat acgagtcatc
    28441 tcacggcgtt aatctcgatc cagataatat tctcataact tcggcgagcc agcaggctct
    28501 cgacatggtt ccaaaactct ttctcgaccc aggagaatat gtaattgcgg agcgtcctac
    28561 ctatcttgga gccattcagg caattcaatc ctatcaagga aaggtgcttg gcattccctt
    28621 ctctcatgcc aacgatggct tggatatgga tgaactcgag aaagaatacg ctcgcgctaa
    28681 ggctgagaat aagcgcataa aatacatcta tgtaattccg gatttccaga atcccaccgg
    28741 agtatgctgg agccttgaaa aacgtaaggc tcttatagaa tttgcatatc gagaagaatt
    28801 attcatcgtt gaagacgctc catatcgaga gcttagattc atgggtgaat ccattccttc
    28861 cttgtaccaa ctagaactgg agatgcagaa ccttggcatt gtggttggcc ttaagacctt
    28921 ctctaagata cttgcgcctg gtacaagaat aggatggatt ctagcgcgta aggatatcat
    28981 tcaaaaactg gtcgttgcaa agcaagccgt ggatctctgc acaagccccc tcacgcagaa
    29041 gattgtcgcc caattcctgg caacaggcaa actcgaagaa gtcttgaaga atacttgcgc
    29101 catctatcgc gataagcgca atttcatgct gcaaaagctg gaaacctata tgcccaagcg
    29161 tccagatctc acttggacaa aaccagaagg tggcttattc ttgtggctct ctctgcctag
    29221 ctacgtcgat accgataaga tgttttacaa ggcaatcgaa aagaaaatcg catatgtggt
    29281 agggagttca ttctattatg atgatcctga agtgaattcc atgcgcatca acttctccta
    29341 ttcgagctac gaacagatag aggaaggagt taagcgcctt gccgatacat ttaaagaaga
    29401 aattgaagag cacgagggtc atctctgaag catgagaccg atctctggcg cataaggtta
    29461 tctcttaggc cattatagtc taagaagaag gattgagtca gaatatacag ctctatattg
    29521 gattaagcgc aacgaacctg caaagatagg tctatctgta tgcactcgaa attataaaat
    29581 cggtgataaa ataatatttt attttgaact catattgtca caacccatag aaattgctat
    29641 ttttctctag caacagctat ctttttcaaa ggtgaaaaac catgccgata attcaggtga
    29701 tcgatgtaaa aaaggactat tatctgggga atgtcgttgt tcccgcactt agaggaatct
    29761 cctttgctat tgaaagaggc gattttgtct ctattgcagg gccttcgggc tcgggaaaat
    29821 cgaccatact caatataatt ggcctaatag acattccaac ttcaggtgag ctaatcataa
    29881 atgagaagaa tactaagggc ctttctgata gagagttgac tcgctttcga catgaagtac
    29941 taggatttat tttccaatcc ttcaatctga ttcctgtctt gaatgtctac gaaaatatcg
    30001 aattcccgct gctgctcgga aagaccaaaa tccccaaaag cgaatggaaa agctggatag
    30061 attttctcat agacgaagta ggtctttccg attggcgaaa tcataagccc aatgagttat
    30121 ctggaggaca acgccagcgc gttgcaatcg caagagctct tgttactaag ccttctattg
    30181 tactggccga tgagccaacg gccaacctcg attccactac cggcgaacag ataatcgacc
    30241 ttatgaaaaa aataaaccga gaattggaaa caacctttat tttttccact catgatgcaa
    30301 agatcgtcaa cattgccgac catgttattc gcttgcgcga tggcatgatc attggcaata
    30361 agcgaaatgg tgaagatatc cttgaagaaa acgcatctat cgcgtctgcg gagaattgag
    30421 catgcctatc atctttaaaa tagctttccg caatatcaaa gcccatagat ccaaaagcct
    30481 catcatcgga gtcttgttag caatgggagc catggtatta gtacttggca cagcatttat
    30541 caatgcaacc gaggaaggag tgcgctcaac attctcagat gtatatactg gagatatttt
    30601 tatctccgga atttcgagtg atggccctgt atctctgttt ggcgtgactt ctacaagtgg
    30661 catggcaacg acgcccataa ttcctgagta tgacaaagtg atttccatcg taaaatcgac
    30721 tccacatcta gagaagactt cttcgctcgc aacgggcttt tctcaggtaa ttcgcgaaaa
    30781 tggtgatcag gaaacagaag aagactctca aaagaagata aaacctcaag atcggtttct
    30841 gttccttttt ggaatcgatg cagcgagcta ttgggatctc tttgaaaccg tagaaatcgt
    30901 tgcgggagag aaattgaaac ctgggcaatc tggcatcatg atcaatgaga gccaattcca
    30961 taaattctcc gattggttcg aaaaacctct ttcgataggc gatcctttgc ttattcaggg
    31021 tttttctaca atcggtttcc gcttgaggga acttccaata gtcggcattt tccggcttaa
    31081 agaacagggt acggcacccg aacaacttgc ttatatagat atcgaatctc tccgcgttat
    31141 gagcggtatg actgttggag ccaacgaacc aatacaactc aagcccgaac agacaagtat
    31201 gctttcgata gaagatcccg atgagctttt cagcgatgat ataatcgtga cttcggagac
    31261 ttcaaaacat ttcgatgtcg cttcaatctc tagggaatta tccaggccca gcgaaataga
    31321 tcctctaaag cccgatgaag gagcatggca gttcattgta ggtaaggctg attcttctca
    31381 gaatatagat cgcatcatct cttctttgaa taaggctttc gaaaaagaag agatccaagc
    31441 tattgcaggt aactggcaag tagccgctgg cccatatggt cagtcggtag atattgttcg
    31501 gattatattt gctgttgcca ttgttatcct ctctatcgtc gccatcataa tcataatgaa
    31561 tacctttgtt atttctgtaa tcgagagaac aagcgagatt ggcaccatgc gcgccattgg
    31621 agctggaaag ggtttcgtgc gcactatgtt tgcggctgag gcattggctc tagcattggt
    31681 ttctgcttcg attggggcag gactaggcct cctgattatc gaaatcctta aatcgctccg
    31741 aatcgaggcg acaaatccat tttttcttgt tctatttggc gggcaatact taagaccaat
    31801 tgccacggca ggaaatgtaa tttttgcaat aatactgatg atagtggtag gctttattgc
    31861 ccacctttac cctgtttcgc tggctttgaa aattcagcca gtaagagcga tgcaggaaga
    31921 ataggaggga atacatgaat ttagtattgc tccgcatcgc tgcacgaaac ctaatgcggc
    31981 aaagaaagag aactatgctc cttgcgggag caatagcctt tggaataatg atcgtaacga
    32041 tcctaaacgg gtttgccgga tcatttgtca agaatatctc agataatttc tcgtatttga
    32101 tgggcggcca tgtcttcgtt agtggaactg aggttacacc atcgggcaaa agaatttcga
    32161 taattcgcga tgattcggtg attatgcaag cattagaaga ttctggacaa aaatgggtca
    32221 gcttgcagaa aaccagccag accatagcca cattgatctt cgagggcaag agtatacagc
    32281 agacgctgac tggtatcgat ttgactcatt ccggaatgat aaaagaacgg cttgctctcg
    32341 tcgacgggaa ttgggaaaat ctctcctggt ctaacgcaat cataatatcc cagagcattg
    32401 ctaaaaaact caatgtcctg cctggagatg tgttggttgc acagttgcag accataacag
    32461 gccaaaacaa tgttggagaa tttcgagtcg ctgcaataac cattgattca agctttgttg
    32521 gctctattat gacctatgtc caacttgaat atctcaatag tcttataggt ctgggaaaaa
    32581 atgaatatat gtcgcttggt ataatgctgg aaaaactgga gaaatccgga gcttttgcca
    32641 atgctctcta tacttcgatg aagggtatgg ggctcaacct tttcgatcgt tcaacaaaag
    32701 atgaaaatgg ttcgatgaca ccatttcagg ctatgcttct cgagcagaga acagaacaat
    32761 ggaccgggac taaatatagg ctttatacta ttgatgatgt gcttgcccag gcaaagcaga
    32821 ttgtagttgc gatcgatacc ggaagcacag tgattttgat cgtcttgttc gccattgtga
    32881 tgattggtat atccaatact ttccgaatgg caatgtatga gcgaatccga gaaattggaa
    32941 caatgcgcgc tattggtgtt caacggaatg aaataagatc tatgtttcta tatgaggcac
    33001 tattgctttc gctggcaggt gctattgcgg gaatagtctt ggcacttatc gccatgggct
    33061 tgttgacgct tatcagtttt gatccgcttt cgccagtatt cttgattctg aaacgcggtc
    33121 atttttcatt ctatgtgccg ccgcttcgtg ctataggcaa tatcctcctt atcgcggtgc
    33181 ttacactcgt tgcagtatat gcacccgcaa atgctgcggc aaaattgcca ccggcagaag
    33241 ccttgagaac agtaaaataa ataggagtct atcggcatgt attcgaatgt atcccggaag
    33301 gcaaaaagcc ttaggagagg gattttatcc atcttgtctt tattattgct gttttttacg
    33361 ggattgaatc ccctgctagc ccaatcaaag ccggatccca gagagctcat caagcaagtc
    33421 gatgatctga gcgatttttc tggcaaggat ttttcagctg ttttcaccat tgtcactcaa
    33481 aaaccagggg agaaagacag cgtcactcag gcccgagttt tccgcagaga tgcaaaaaag
    33541 caattcgtaa ttctgatctt gctcccggaa gtcaacaaag gacagggtta tctcagagaa
    33601 gatgataatg tctggtttta tgatccaaca agccgcaagt tttcgcattc ttcgatgaaa
    33661 gagaatatac agaacacaaa agcgaagaat tctgatttta ctctcagcac ctttgctgag
    33721 gattataccg tgacaaaaat gaccgagagc atggtaggga aatttccggt ttgggtattg
    33781 gatctaaaag cgagaacaaa tgaagtagca tatgatcggg tagtgctcta tatccgcaaa
    33841 gacaggacaa tgttgctcaa acgagaagac tattcggtaa atgggcgcct tatgcggact
    33901 actgcatatc cgaaatacgt agagctggaa gggaaactcc ttccttcgca gatattgatc
    33961 gttgatgaag tcaacaaagg agaaaagagc cagatgacca tgacggaaca atctgtgtct
    34021 cccttgccag atagggtgtt tacaaaggct ttccttgaac aagtaagcag ataatccagg
    34081 aggtaataaa ggaggatgta gcgatgagtc ttcttcggaa cttaggtgca atcggtattc
    34141 tatgtgtgat tattatggct gcgcccttca gcatatcatc tcaagaagag agcaatgata
    34201 tcgatgcgct ctttgaggat gaaataatag ttcagcaaga cagcgatact ctcggtcaag
    34261 aagaatccca agattcattc ataggacaga caaatccact tcagagtttt ctaaaaaccg
    34321 agactgtaaa gatcggcgga aacataggcg ggactgtgag cgcttcctgg aaatggaatg
    34381 atccgtggac ggtgggtttt gatctcgcaa atccagattc acatgaattg gccccagcct
    34441 tgaatagtct tttatacttc gatgcacggc cagaggagga tttccgcgta catggctcgt
    34501 tcaagaccgc ttggccgttt gcagagacaa agagcctctt ggattccaaa aacaaaacta
    34561 cattagtctc tgtcccagat gttcgcattt ttgaactgtt ttcggatttc cagcttggag
    34621 ataatgctta tctgcgattc ggaaaagcca cggttaaatg gggtgtcgga tacttcttta
    34681 gtccagcgga tattatcaat ctggagtcta tcaatattct ggatccgacc gtgcagcgcg
    34741 aaggcccact tcagtttcga gtctttgttc cttatggacc aagccagaac actctgagct
    34801 tttattccat ttttgacagc aacaaatggg attttgatac tactgcgctt gcagcaaagg
    34861 cagaatttct tcttggcaac tatgaattga gtttgaacag ctactatcga tatgatacta
    34921 ccgaacgcgc cgctttgaca ctaaccggtc cgcttggcaa tttcgatatt tttg
//
LOCUS       DAOMZL010000007        34135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_948_length_34135_cov_14.113058, whole genome shotgun sequence.
ACCESSION   DAOMZL010000007 DAOMZL010000000
VERSION     DAOMZL010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 34135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34135
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_948_length_34135_cov_14.113058"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..611)
                     /gene="rsmD"
                     /locus_tag="PLQ30_01300"
     CDS             complement(75..611)
                     /gene="rsmD"
                     /locus_tag="PLQ30_01300"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPW01111.1"
                     /translation="MRITGGEYSGRIVKVPEGDLRIRPSMDRMRQSVFDVLGDLLGYS
                     FLDLFAGSGILGLEAASRGASPVVCVEKDRKKFPVLLQNAAMAKPAVFCKAMPAETFI
                     MRNKQAFDIIFLDPPFDYRFKLQLLERLDRSSSIHDGSLVLIHFPREDNLPQEISGLR
                     CYDMRIFGRSIVHFYRKI"
     gene            complement(617..1084)
                     /gene="smpB"
                     /locus_tag="PLQ30_01305"
     CDS             complement(617..1084)
                     /gene="smpB"
                     /locus_tag="PLQ30_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPW01112.1"
                     /translation="MKTQDSIKIIAVNRRARFDYEIEERLECGIALYGSEVKSIKEGR
                     VSFSDAFAEIKGREVWLNNLHIADYPHSSIFRHDPDRAKKLLLHRQEIKRLERRVREK
                     GFTLIPLSIYLKHGLIKLELGLCRGKKAYDKRAEIKARDLDREIRREFKVKDW"
     gene            complement(1081..2634)
                     /locus_tag="PLQ30_01310"
     CDS             complement(1081..2634)
                     /locus_tag="PLQ30_01310"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPW01113.1"
                     /translation="MKESEKITDQDIEKAKVTLKPIFGIRPRIYIPILFAVLIFIILF
                     FLLVNPGLVNPGAKISFKGNPDVAALYAENTYIGNTSDGIRLRPGSYQIEIRKRGFEP
                     KTIAVKVPNRIFATLILPPRVTIQYELKSESLESILIPSFKEFASWSLSGRPSAIYQL
                     PMVLSEASKDLAAFPVADTVPIAEPLLAAGLSIAQNTSSIRDLIYASFSLGAPGASPL
                     GFISMARSAIALLANSKSYVGAFSEIAPERTDDAIRNALSALEKETSLFETSLYNPVG
                     VQIIGSHSFIIFNEGYLKGLTSTPGGTGIPYIALVPKFGLASTEVTQRQYARFLKENP
                     EWAPENRATLIEKGLVDNYYLADIDISSPSDKPITGVSWYAANAYCEWLCGYAPGGYE
                     VRLPTEAMWEMAASQAYRNIKNLGVFNNRSSNGPLSVASYGRDASGFSDLFGNVWEWT
                     SDSFRPYVWITEDSPAFKMLANAGNSKTVKGGSWANSADQISIESRGPMPADHSSEFL
                     GFRPALIRK"
     gene            2713..3678
                     /gene="lepB"
                     /locus_tag="PLQ30_01315"
     CDS             2713..3678
                     /gene="lepB"
                     /locus_tag="PLQ30_01315"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPW01114.1"
                     /translation="MDILDKLVVFSEDLLTRRKKRSLRAKMKQQKRHPILDWIYAILW
                     AACVVLVINQYFFQNYRIPSGSMEKTLLVGDMIFVDKLSYGPELLPGVLKTPGVSTPK
                     RGNVIVFENPTYLSRGPIYTIFHQLLYMVTFTLVDIDREPTGEPRVHYLIKRAIGVGG
                     DTLRVRNGDVFIRPRGSSVFFYERMLMDGLGLPAKMQRLVESSEYPEIDNVGVASAYT
                     ELGLSLPSRVGMPNVQHANKDAFQYDMVRVTTLRDANPSDYRNAQLAQRYENGWYIAD
                     SRLFPMGDNRDNSRDARYFGPVAEKKVLGHALFIYFPLSRIGSIH"
     gene            3691..4272
                     /locus_tag="PLQ30_01320"
     CDS             3691..4272
                     /locus_tag="PLQ30_01320"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HPW01115.1"
                     /translation="MFAKRSIRYAERIQRKRTRNRILLYIALFIVLCIVLRTFVFQTW
                     KVRGELMKPTLRDGDIVIVLPCLSFSDKMLVSLAGIPQKGDVVLVEDGSESIVPTMTM
                     IEDSALRFMTLQKYSLLAAEFGKGFGLPTVMRIQSIQHDKDISKSQPVYRLATDCQSD
                     KNFMTGDRIVPPSLIKGRVVLKIWPLARIGKVQ"
     gene            4269..5528
                     /gene="hemW"
                     /locus_tag="PLQ30_01325"
     CDS             4269..5528
                     /gene="hemW"
                     /locus_tag="PLQ30_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPW01116.1"
                     /translation="MKAWTSQVFGHERGIFPIIDPFKIRSLYVHIPFCADRCSYCDFF
                     SVPLIKDPTFSTIEYEISYVQATLMNIRNWSKLFGAGPFSTIYLGGGTPSVIDRRALK
                     ILIDDLEQFVDTDCEWTIEANPDSLDKALLDMLAGTGVNRISLGIQTLSEEEWPILRR
                     VGSIDEAVQAIELLCEYPFELSVDLLMGIPNKTNKGRQGHSSIMNSLEYLIGRVSHLS
                     IYDLTLEKGTLIESQVSSGKLIMPDSDELAEERDKVDLYLEGQGFHRYEVSNYAKPGH
                     ECRHNAVYWNMEPYLGVGSGGVSTIQYPMCEESQELGTIIRITETRDISAYIEAPMEI
                     PKDIEIIDKKTALFEFLMMGFRTSRGIDLKRIKSLFGIDVEECIPSALKRWNSEIVRN
                     GDFVALRPDNFDILNRFLVECLEELDL"
     gene            5538..6797
                     /locus_tag="PLQ30_01330"
     CDS             5538..6797
                     /locus_tag="PLQ30_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01117.1"
                     /translation="MAIFQYAVISSILHDEPQKKQLLSVFEDKLQRFGGELHTLESNM
                     IHPRNSELPLFMLVLTGGTESEAMSLISRERMLDLRMPIVLLAHPSHNSLPACLEILA
                     KVKQEGGKGLILQADSSGAFDAEELEDIIAVCKSRITMRSCQIGVIGRPSDWLIASSQ
                     DLHAAMNSWGVRLVEIPLEELVRNIEEIRSSSCGVPVIEDFQEKAEFYAESSNDDILK
                     SIQILQALRILVDKYRLSSLTLRCFDLILKDRSTGCLALSQLADEGIDAGCEGDIPSI
                     IGLSWIRSLTGQIGWMANPSHIIAKKGEDSAEMLIAHCTAPRSILSRYALRSHFESGL
                     GVAISGILPKEEVTLFRIGGKYLDKLWYSPARIIASPRDEGLCRTQALINLPNEKAQE
                     LLINPLGNHIVMVRGNWTRRISISAQL"
     gene            complement(6813..7421)
                     /locus_tag="PLQ30_01335"
     CDS             complement(6813..7421)
                     /locus_tag="PLQ30_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW01118.1"
                     /translation="MKIYHHYSVYGFSNIYTIVNESLKKAIIVDPSEITPTMIERIEQ
                     NELDLCGILVTHDHIHHVNGLSTIMKIYSPTIYASDAKLLGFQCRKLRDGDAFSEAGF
                     DITAIAVPGHSQDSIVFLMEGYFLFTGDVLHAGVIGKTNSVFNTNALIARIKQKLFCL
                     PESTLIFPGHGPPSTIGTEKSANISFQEGFAERIRPNYDFFV"
     gene            complement(7418..8017)
                     /locus_tag="PLQ30_01340"
     CDS             complement(7418..8017)
                     /locus_tag="PLQ30_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPW01119.1"
                     /translation="MFKVSACSIEDCSLPAFSLENLCYYHLQDYPERMKKAMQELQSK
                     NILLNRVFDNAFLMGLDFSRYRFVGCSFRNARIQHSLFTGASFHLCFFDSSSFFSCDL
                     SGADVDFCSFGKCSFMDCSFENSELIQSCFNGSVIVDCTFAGSNLYNSRFIMCDMNTV
                     NIDNCDFKRAFYIPSKEQNVSFSRSNIAEAVRDLEHLYL"
     gene            8155..8445
                     /locus_tag="PLQ30_01345"
     CDS             8155..8445
                     /locus_tag="PLQ30_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01120.1"
                     /translation="MCYKCGSYLAYDYRPGFNDTCDICGKDLHACLMCRFYTPGAHWD
                     CRETIEELVQDKEKRNHCEFFIISEKYLEKGETAGSKQDDDAKRRFNALFGE"
     gene            8467..9264
                     /locus_tag="PLQ30_01350"
     CDS             8467..9264
                     /locus_tag="PLQ30_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HPW01121.1"
                     /translation="MRICVFDSGIGGLPFVQAVNREFPFLEIDYVADDAGFPYGTKSP
                     EMIRDILFERFRRIRARIDPEVLILSCMTAVQIGLKELQAAHRTLQIIGAIPPIAEAA
                     NESQTHRIALLATSRTTEDSFLDYLIARDAPDCEVIRIPAQDLIEYAEQRLPFASKLS
                     AQTMVAPYINYALKEGTDRIVLASSQFILLEDSITNQLNELQAFNVKCLDPRGKVICS
                     LQKLIPDKEHSEGQFVKPRFYLTGDQGANPSLIAWSERFNCSKPQLL"
     gene            9261..10268
                     /gene="rsgA"
                     /locus_tag="PLQ30_01355"
     CDS             9261..10268
                     /gene="rsgA"
                     /locus_tag="PLQ30_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPW01122.1"
                     /translation="MNGNSKESTVLGVVVSFSNRNAKVRCSDGNILTCSLKGKITDSS
                     FQGYKSPVAGDHVSVIPISKQNGVIESIFERKSELSRYNEKGETKQVLAANIDLVVCI
                     ASTRAPPFRPRFIDRVGVMAEYCGLPFLIVCNKIDLGLDVSIESRLAAFQKLGYGVVK
                     SSVRDSIGIDELRTSLLGKTSVFIGQSGTGKSSIINCLIPGADQRIAEISDKHKRGKH
                     TTTLSAMVSSSNGDIDIIDTPGIRRLAIRNIEPDDLGFYFPDMVPFLGLCEFGASCTH
                     RYELHCFVKQAVQEGIIHHDRYESYLRMRAELEETRNTKTNESRKPLRSATYKFEDEE
                     W"
     gene            10262..12754
                     /locus_tag="PLQ30_01360"
     CDS             10262..12754
                     /locus_tag="PLQ30_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPW01123.1"
                     /translation="MVTNQHSLALLDFYRLRDRLEEYCFSHEGRALLNSTLPCSQENS
                     VKRLKKDLSLLVEGLNEKEIPSFPFPNIENPIIKAAKEGTVLEAEELFAIGLWVKSYH
                     QLFSFLRRILDSFIEESKEVSAENSMDGEIRQEDSINDESWAEWGIENIVYSAPALSQ
                     VTQMIFAVITEDGEIKDLPRLKALNLAIQRTQAENLHIINRYLSDPSLQDALQSNIAT
                     ERDNRVVIAIKANFKGRIKGIVHEFSSTGQTIFIEPIELVERNNKLLELESKLNEEIR
                     AILRETTAALRHFLPILMRARGFLAAADARFARAIQVKREGLAIAGHLEHGIHLYQAK
                     HPLLGKKVVPIDVDIPDDSRILIITGPNTGGKTVSLKTIGLLALLNQYGAGIPALSTS
                     AFAVFDTILADIGDEQSIDQSLSTFSGHMKVISEIISSATDRSLVLLDELGAGTDPEE
                     GCAIAMALLDYLYRLQCFAIVSTHHGVLKNYGYTKPGCLNASMEFDAESLSPTYKMIM
                     GVPGESRALEIAGQIGLPMEIVDIAKSYLEDERSDYSMIIHSLGEKQRELEKLELEKK
                     KQLGKVLELRRKVDLENLRIRRRELELRRQGIAELKHFINESRRIFENIVRELRENGK
                     KDGDTSAGREFLKEISDEIHRQSETLESFAQETNRIERSLKPEMQGNIGIDIEKMKFG
                     DTVIFHNREAVFLRKLDKKNALVQIGSLRVPIEVESLSLPKTMDRFDKAAKPDYQIEL
                     ASDSMGRNAPVAELDVRGMRLSEALDLLEHQIDAASLAGVSSFSIIHGTGEGILSRGI
                     HEWLKKQISVADYYFARPEDGGFGKTWIHLKE"
     gene            12902..13636
                     /locus_tag="PLQ30_01365"
     CDS             12902..13636
                     /locus_tag="PLQ30_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPW01124.1"
                     /translation="MSGHSKWASIKHKKGALDAKRGQLFTKLIKEISIAARMGGGDIE
                     GNPRLRTAVLKARAANMPKDNIDRAIKKGTGELEGSIYEELFYEAYTQGGGAMLIEVL
                     TDNKNRAAAEIRNIITRSGANLGTTGSVAYLFKRKGILTYDSEKYTEDQIMEAALEAG
                     ADDVVSEDGTISVYTDPSSFEDVLNVMNDKGFETLGAEISMLPDSYITVDLETAQKIQ
                     KLIDKLEENEDVQSVYHNIEMPELEE"
     gene            13633..14151
                     /gene="ruvC"
                     /locus_tag="PLQ30_01370"
     CDS             13633..14151
                     /gene="ruvC"
                     /locus_tag="PLQ30_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPW01125.1"
                     /translation="MIGALDSRSSLVAIGIDPGIAAVGYGLVRTLEGRLSLVTYGCIE
                     TESDEPIGKRLTYIFEEIRKLLGQYAPDIGGIEELYFFRNVSSAFPVAEARGVIRLAF
                     EKTGIALKEYSPNAIKKAVTGSSRASKSQVQEMVKILLGMSQIPTPDHASDALAAAIC
                     TLHSIGPAGYTL"
     gene            14153..14758
                     /gene="ruvA"
                     /locus_tag="PLQ30_01375"
     CDS             14153..14758
                     /gene="ruvA"
                     /locus_tag="PLQ30_01375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPW01126.1"
                     /translation="MFNRIKGVFSGHEDEALHISTGALEWEILVPIRDPSFYNHTGEE
                     IELFTWLHHYEDAMRLYGFFSALERKLFLDLQKVEGIGPRQAFKILQGIAPRSLVTML
                     DSGDLASLQKIPGIGPKTAQKMMLALKGKLITQEEASAVISGSQGRFRDVVRALVEMG
                     FERKEVERVTAQLASSLEDSPEAEKELFKRALLELSAGAGA"
     gene            14797..15801
                     /gene="ruvB"
                     /locus_tag="PLQ30_01380"
     CDS             14797..15801
                     /gene="ruvB"
                     /locus_tag="PLQ30_01380"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPW01127.1"
                     /translation="MNPLYNPAEDDKEASLRPRYLAEFQGQQRIKDNLLIFIKAARDR
                     GEALDHLFLMGPPGLGKTTLALVVASEMGVDFKPTSAPAIEKPKDLAGILTSLRPGGV
                     LFIDEIHRLKPSIEEMLYIAMEDFELDWIIGQGPAARTVRIPIPQFTLVGATTKAGMV
                     SAPLSSRFGISLRFDYYSPQELASVIRRSSNILDIKINEDAIELLSGTSRGTPRIANR
                     LLRRMRDFAQVRGEGIITRDITLDGLERLEVDYLGLERLDREILKTIILKYGGGPVGA
                     ETLAVSVSESIESLEDYYEPYLIQIGMLARTPRGRVATQTAYRHLGIERPNENGQFRF
                     "
     gene            15776..16885
                     /gene="queA"
                     /locus_tag="PLQ30_01385"
     CDS             15776..16885
                     /gene="queA"
                     /locus_tag="PLQ30_01385"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPW01128.1"
                     /translation="MKTDSFDFDLPEHLIAQYPTEQRGRSRLLVLDRKTGNTIDSMVE
                     DIVSFIEPGTLMVFNDSKVRKARLYGTCEQTGTIVEFVFLSPWKQDDPTSLGDSSSDH
                     ESQGDLVKACKPISTVSRLWRAICNKSKQQKIGRSYMFPGQLRGSIIEDRGHEKTIEF
                     SFIVGDEYFDLYGHVPLPPYIKREDTSDDELRYQTVYAKHTGSAASPTAGLHFTPQLL
                     DELSRKGIEIEYLTLHIGLGTFQPVRADNVEDHQMHEEWFDISLEAADKINLALSEGR
                     KVLAVGTTSMRALESAWYGGKVKHGNFSTRIFIYPGYEFHVVDSLFTNFHTPKSTLLM
                     LVSAFAGREKILECYRLAIEKNYRFFSYGDAMLIL"
     gene            complement(16948..17589)
                     /gene="tmk"
                     /locus_tag="PLQ30_01390"
     CDS             complement(16948..17589)
                     /gene="tmk"
                     /locus_tag="PLQ30_01390"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPW01129.1"
                     /translation="MPTYNTDELKNRFIVIEGIDGAGTTTQVRLLGETLIARGFSCMV
                     TAEPTMNHIGQLIRTILSGEDMVVPTTVAHLFAADRNEHLYGKNGIMESLKSGSLVIS
                     DRYVLSSLAYQGTTCGHRLPWLLNSAFPAPGLTLLFEVDPEICISRIRSRLKKEIYET
                     LEFQRRVQTMYESMAYRLSNKGWRIERIDGSKDIESVQRQIESIVFAFLGFEC"
     gene            17643..23576
                     /locus_tag="PLQ30_01395"
     CDS             17643..23576
                     /locus_tag="PLQ30_01395"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HPW01130.1"
                     /translation="MRTKKSVIVIVIFSLALAIWGSIVIADLVQREVQMLAIKALDRL
                     QSAVVDATGLILKYDNLDIESLNRFSMHGIALIRSDAAEHNGNKPSSYSEFYPSSERS
                     NYEEQPPLRIKKLSIRISIWALFSGHNNEAVRDITVDDVQISLYLPNDEYIIKKILEN
                     FVSGKAESVPHFELSIGPVRAEVREIAKSSNASMDNNVINEGSLTIESLQFSSLTDSP
                     VIIAPSVILKTSGLLGLPKDLNAEFGLRGSGNANLSDIDMNIDLEASSTGWHIMPQKL
                     LLEKRGKRIECKKSGGGIELSGSYEGGVWGIEGVFEGYGVGEDIELGGEWGEIVKGSE
                     VSGEIGVGGDLEGLSRYEVDLGIDIGVGISIGGYDLGALGVSLIGVGDAVSYIGGIRV
                     EVGGNEFRYEGEVGYEGIRLGGNYELSGVDIGIGGYIRGGIGEYEVGVGKGELKGVEI
                     GLGVCRVRVGVEGYEVRYEGAVGGGEVKVGGSIGKAGGYEGVVELEGVGISEVMGALG
                     VGGIEGEVSGRVYGLERGGKRSWSISGLAYDGEVMGAEVSLRGDGVGDEAGYEIKVLE
                     ASIGDYDIALRGKGSYVGELFSGEFSAGGMSYEVSVEARGGKVRVGIGEGFRGAFELV
                     GGRLEGEVGMDKFGLGLGEGMIRVGGLASGWYEAGGWGISLEGMSLSYEGEGKYPEVK
                     VSGLIGDSGIRFEWLEVKGDGYGFKGSIDLKGEGGEYILIGSGKGEGVLGEGSEYSVY
                     GVIRGKGVDLRVGVQGLGVGSYVVEGYADLRGEVDIREVLKGSLEGIGDIRGEVAVGL
                     SGSGLRVDEQRFEVEKRGKRIECKKSGGGIELSGSYEGGVWGIEGVFEGYGVGEDIEL
                     GGEWGEIVKGSEVSGEIGVGGDLEGLSRYEVDLGIDIGVGISIGGYDLGALGVSLIGV
                     GDAVSYIGGIRVEVGGNEFRYEGEVGYEGIRLGGNYELSGVDIGIGGYIRGGIGEYEV
                     GVGKGELKGVEIGLGVCRVRVGVEGYEVRYEGAVGGGEVKVGGSIGKAGGYEGVVELE
                     GVGISEVMGALGVGGIEGEVSGRVYGLERGGKRSWSISGLAYDGEVMGAEVSLRGDGV
                     GDEAGYEIKVLEASIGDYDIALRGKGSYVGELFSGEFSAGGMSYEVSVEARGGKVRVG
                     IGEGFRGAFELVGGRLEGEVGMDKFGLGLGEGMIRVGGLASGWYEAGGWGISLEGMSL
                     SYEGEGKYPEVKVSGLIGEQGADLDVDYLQYAGQSLKGSMIIQYSSIANLLKDTKIDY
                     SFLDIKTDRTILEGGIVALGDKLEVAIKTSSIPLEEVFPSTFNISGDIELKGSSTISI
                     LSKKLDLSALSSLNLQFNTRKSEMKGIPFSAAGTINFKDCTFSLESGIFSYQNYRFEN
                     INAEYNLESKQVAYALNSKIVIGEQLLSANLKGFGDNIDSSIFKLGAAQAFHITGEIN
                     DASLGSKTIDPLSYSISNADDKLDIHLSQANGDSIDASITNMTDFDLVLNDLFKIKGR
                     ANGKIQAKEVWADIALEKIDLKTLGALISKDDLGDTSGIASAELNLSGDISDPRIEGK
                     ILFSNLIIDQNLYIVEKIGPFDAEIAINDGVAELSPTIINIGTGDISLAATANLARWQ
                     LSDITVLVSTLEKASMRFKGKIGGLNTDGIWIKGDIKAAISSNSIEIGGDMLLDDGTL
                     EVDPNGFISSENAQTPVSPLYLKLMVTFGKNVQMYIPGKDISLIRGTISPASTFTVQY
                     DQARGDFAVNGRIELRSGYVYYLLRDFFIKKCTIELAESQSKFNPVMDVIAELMEPTK
                     DGMIKIILSADKSPLDNFSPVLSSVPPKSQIELLALLGGGLALSELAEDTPLTLREAV
                     IASSEFLTHNLIFRSFEQRIQKALGLDVLYLQSSFIQRWLLDITDQTRENQTNLSRYL
                     IGTELFGGKYITDSAFVHFSLMIEQDPLEKTGFLQLNPKVGLELQSPFGLLQWSMSFG
                     KEGMPLNNQELSLSWRINF"
     gene            23595..26147
                     /gene="bamA"
                     /locus_tag="PLQ30_01400"
     CDS             23595..26147
                     /gene="bamA"
                     /locus_tag="PLQ30_01400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HPW01131.1"
                     /translation="MRYSHKHFIVIIMIFCSFQIILAQTSTQELAGQDYSQDWFWNKK
                     IAGFRWDGLHYANRNDLDSMLRDYIGTLFTENIWIEIQAKLYALDWFESIESMALPAG
                     EEEDKIIIKFIVIEKPSINSIRVIGNSSIRTSELLDVASSKTGAIFNEDKAKADSLAM
                     QKLYLQKGFPDARVEYEKTEVEGDSNLIILTFKVTEGTAVVVKKIAFSGISAFSSQTL
                     KGQVTLKEAGLFQKGAFEESKLAESRTALEDYYASKGYIDAKVIDVIKNYSKDEKSQK
                     NYLDITFVIAEGKQWKLGDIFFEGNSIFSTSRLASLISLKQNEPINLKRLLTDKQKID
                     DLYYESGYIFNTITMTEIRDEQGGRISFKISITERDRAHIESISFKGNTKTKDYVIAR
                     ELPLEAGEVFSKAKIIDGLRNLYSLQYFSSIEPEIHQGSVVDLMDLVINVEEMSTAQI
                     QFGLTLSGLGQPSAFPISGFIKFSDLNLGGTGKNLQVNMTLSPDEQSLETSFGQSWLF
                     NKRISQSISFGIEHSVLQTAQDCIAPIFSEEDIPDPFIGLGSGSDEWDGLLSSVPDAY
                     LMDYDDYSFSLGYSMGYVHKIPQGDVGIAGGVSSGLDMSSYDTAKYRPYEKEMRDYNG
                     QWVLKDSIFGRVYLNRLDYWYNPGKGYFLSQRLTYTGILPMERQFYIRSDTRAEFYAT
                     LFSIPFSSSWIFSPVIGIHSGVQALLPQPWNSLAVTKDWVYIDGTFNVRGWKSLYKGH
                     GILVWENWLELHLPIVPQVLSFDGFLDAGAMMTENGWLDMTLDSPVSKGSNALEWNNF
                     AFSIGFGARFMIPQFPFRFYLAKRFVYDGSKVEWKTREGSFDFVLSITQPLY"
     gene            26194..28812
                     /gene="mutS"
                     /locus_tag="PLQ30_01405"
     CDS             26194..28812
                     /gene="mutS"
                     /locus_tag="PLQ30_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPW01132.1"
                     /translation="MLLEPAFYNGAMSKNPDSMLDQYRRIKAKHRDEILFFRLGDFYE
                     MFNEDAKEASAILGLTLTKRQGLPMCGIPYHAAKTYIARLLKAGKKIAICEQKSLSNK
                     RGLLDREVVEVITPGTALEDDYLEQGTNNYLADICIIDQRLCFAYLDVSTGEFRAFSL
                     TSNKEMVTETIRAELYRLAPREILVQQSILDQPKFYQVIRECSAIIEAQPDWVYDLVH
                     SLDLLKKKFGVVSLKGFGFEDNAPEIAAAGNLLEYVQEMIRQDCPHIRALLPYEEREF
                     VLLDEATKRNLELMRNLSDSSKRDTLIEVIDKTKTAGGARLLRQWLFQPLRIREKIEA
                     RLEAISFLYHNQILLADIRKLLYGILDIERLISRLAVDKAHARDLRAIRDSIDLGLDL
                     FTLIESAKAPLELGPRMSMEQIDACRQVIDIIEKTILDDPSILLTEGNLIRDGYDKEL
                     DNLRNLHKNTQEILEDYLKDEKRQTGIQNLRIRYNKIIGYYIEVTKGNIANVPSHFVR
                     RQSLVGGERYTTDRLAELESKINGAQERIIEIEKNVFLKTRDSLKQFIPALLECASEI
                     SRIDCLASLAQTATEQGFTKPLITEDEDIEIVNGRHPVVEICLPYGDFVPNSIALSRK
                     NNWFVLITGPNMAGKSTVLRQTALIVLLAHMGSFVPAESAKIGLVDKIFCRVGAQDNL
                     ARGESTFLVEMHETAFILNTATEKSLVIMDEVGRGTGTLDGVSIAWAVSEYLTSRISC
                     RTLFATHYHELTTMAIPGIRNMRMVAIEQDGNISFPKRLEDGASSGSYGIHVAKLAGL
                     PKEVVIRASQVESHFNALEQGLKEEKPKEDSTPSLELFSNEDIVIETLRNIDPDNITP
                     IKALQILSELVERLKQ"
     gene            29043..29558
                     /locus_tag="PLQ30_01410"
     CDS             29043..29558
                     /locus_tag="PLQ30_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01133.1"
                     /translation="MDCRNAQTLLAGIIPVFSFRGNAASLIHKFKTGQRISLAHYFAY
                     RISMLDELTADVASSTCLVPVPPRPEKLRSGQLDQVGAIAESLINFGFHYEKMLLRLP
                     GSSQQKMLDRAGRLKNASQSYALLSNKKTIAKIILLDDVCTTGATLEACAKLLMENGN
                     KVVSAIVLAAD"
     gene            29904..30395
                     /locus_tag="PLQ30_01415"
     CDS             29904..30395
                     /locus_tag="PLQ30_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01134.1"
                     /translation="MRKKKGGWKGMIDKELQEEIDATLKAGGLDLLEFSLTRQKGGLR
                     VKAVIYRPEGTGTDECARAYRLILPRIQMALGVQDPYIEVYSPGIDRVFRTEREWIAF
                     KNCYIKFMTSDSHDWKKGRILHFDNGKVQIEMDDTILNIPIASIIKAKLDSSRKGEKV
                     NGI"
     gene            30385..31821
                     /gene="nusA"
                     /locus_tag="PLQ30_01420"
     CDS             30385..31821
                     /gene="nusA"
                     /locus_tag="PLQ30_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPW01135.1"
                     /translation="MASEMAEAIHQLISEKGIPEDLIVKTLEEAILAAYKKKFGTIDN
                     AIVRMKENYEGIEIYAKKTIVSDEDFEDEVLEIPLEEAKKFADEAEIGDILEISIDPS
                     EFDFQSVMLAKQTARQSLRDISRDTLYAEFKSKVGEIVIGYFQRERNGTIFVDLGKIE
                     GIFPRKYQSPRETYHVGDRIKALIVEVEKTKTGFQVVLSRTHAEFVRKLLELEIPEIY
                     DKTIEIFKIVREPGYRTKVAVYAKRSDVDPVGACVGPKGMRSQLISQELEGEKIDFIR
                     YDINPKEFIKNALAPAQVKEVYILDEAKHMALAVVDEHELSIAIGKSGQNVKLANRLC
                     DWNIDVMTEEQYLQDSRNIELKKAADRLFSVEEEEGGEFLIRDLPNLEQTWIDALESA
                     GIRLIEQFLELDEEEIARNTALSATDISAIRDIIEDNVEIIQEEVEEEGIVSYSCPEC
                     GSPITIDMDHCPSCGVALSFEAEEEQDE"
     gene            31831..>34135
                     /gene="infB"
                     /locus_tag="PLQ30_01425"
     CDS             31831..>34135
                     /gene="infB"
                     /locus_tag="PLQ30_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPW01136.1"
                     /translation="MTESNDKDLKKVHLIKQPRQDSPEQPQEMSAQNPLDQGAEKKKV
                     VVVVKKKTITTKKVQAKAMIPGTPKDQKIDEEQSALSTQPHSEVGQNKAQIAKEDQVA
                     SPSPITPSTTTLPSSKSPSNVPSMKLSEHKQEIGQDETIISSQRRYSSDGRAGNLAQG
                     RPSSTFRNSHDRGSSRPANGSYQGRAYGQPSGRSYPSSSSRSGQSGSQQFSRGNRPSG
                     GFQSQGGTGSGGRTAYPYAKTGQPGSGQRPGGYIGARQGPAGGRYGLRQTPAPVPSTG
                     NERPASRRAPVKKKGVFNKREEETELEKQIQLKKKAEAKLAAVPKSIDIMENISISEL
                     AKKMNLKPADLISKLMSLGVMATINQKIDADTAAILAAEYSCEVKVVSLYDETVIEKA
                     VDRPEELRPRPPIVTVMGHVDHGKTKLLDAIRKTDVVSQEFGGITQHIGAYMAEYPNG
                     KISFLDTPGHAAFTKMRARGANITDIVVLVVSATEGVMMQTKEAIDHAKAANVPIIVA
                     INKIDLPEANPDRVKTQLSELGLIPEEWGGQTQFVEVSALQKIGIRELFDAILLQAEM
                     LDLKANYDRLAEGKIIESRIDQGRGIVATLMVQNGTLHVGDPFVAGIFPGRVRAMFDD
                     KGNRIEQATPSMPVEVIGFEGMPEAGDPFEVVEDEKFARQISTKRQELKKYEEGRNVK
                     KVTLDNLYETISQGEIKELKVIIKGDVHGSVEALKGMLEKLSTSEVHLNVIRAAAGAI
                     TEDDVMMASASNAIIVGFNVRPTSSAKL"
BASE COUNT        10145 a         6443 c         8439 g         9108 t
ORIGIN      
        1 ctttttcggt ttttattata ccttttagtt catatcaatg caatattttc agttgtttct
       61 taataaataa aatatcatat ttttcgatag aaatgcacga tagaacggcc aaagatcctc
      121 atatcatagc atcttagtcc cgagatttcc tgcggcagat tgtcttcgcg aggaaagtgg
      181 attagaacca ggcttccatc atgaatagaa gaagatctat cgagtcgttc taacagttgg
      241 agtttgaatc tatagtcgaa tggtggatcg agaaagatga tgtcgaatgc ttgtttattg
      301 cgcataataa aggtctctgc cggcatggcc ttgcagaaaa cagcgggctt agccatggct
      361 gcgttttgta ataataccgg gaattttttc ctatcttttt ctacgcacac aacaggacta
      421 gctccgcgcg atgcagcttc gagcccaagt attcccgaac ctgcgaaaag atcgagaaat
      481 gaataaccca gcaggtcgcc aaggacatcg aatacagact ggcgcatcct atccatcgaa
      541 ggtcttattc tcaagtctcc ctcaggcacc ttgacgattc ttcccgaata ttcgccgcct
      601 gttatacgca tcgaacttac caatctttta ctttgaattc tcgccttatt tcgcgatcta
      661 aatcacgagc ctttatttca gcccgcttgt cgtatgcttt tttaccgcgg cagagaccta
      721 gttcgagctt aattaacccg tgtttgagat aaatgctcaa tggtatcagc gtaaagcctt
      781 tttctcggac tcttcgctca agtcgcttga tctcctgacg atgtagaagc aattttttag
      841 ccctgtccgg atcgtgacgg aaaatggaag aatgaggata atctgcaata tgcaaattat
      901 taagccagac ttcccgtccc tttatctccg caaaggcatc gctgaaactc acccttcctt
      961 ctttgattga ctttacctcg gaaccataga gcgcgatacc gcactcaagt ctctcttcaa
     1021 tttcgtaatc aaagcgcgcg cggcgattaa cggctattat ctttatggaa tcttgcgttt
     1081 tcattttctt atcagagcag gcctaaaccc tagaaattca gaagaatgat ccgcaggcat
     1141 agggccacgt gattcaatag agatttgatc tgcactatta gcccatgagc ctccttttac
     1201 tgtctttgaa ttccctgcat tggctagcat tttgaatgca ggcgagtctt ccgttatcca
     1261 tacataaggc ctaaagctat cagaagtcca ttcccaaaca tttccgaaga gatctgagaa
     1321 gccagatgca tcgcggccgt atgaagcaac cgaaagcggc ccatttgaag aacgattgtt
     1381 gaaaacccca aggttcttta tgtttcggta agcttggctt gcggccattt cccacatagc
     1441 ttctgttgga agccttactt cataaccacc tggtgcatat ccgcaaagcc attcacaata
     1501 ggcattagca gcataccagg agacaccggt gattggctta tcgcttgggc tcgatatatc
     1561 gatgtctgca aggtagtagt tgtcgacaag tcctttttca atcagagttg cgcggttttc
     1621 gggcgcccat tcaggattct ccttcaagaa ccttgcatat tgtcgctgtg tcacttctgt
     1681 gcttgccagg ccaaatttgg gaacaagagc aatatatggt attccggtgc caccgggtgt
     1741 gctggtaagc cctttcagat atccttcatt gaaaatgata aaagaatgag agccgatgat
     1801 ctgtacgccg acgggattat acagagaagt ctcaaaaagc gaagtttctt tttctaatgc
     1861 cgatagagca tttctaatgg catcatccgt tctttcaggg gcaatttccg aaaatgctcc
     1921 tacatagctc tttgagtttg caagcaacgc tatagcactg cgggccatag aaataaatcc
     1981 caaaggactt gctccgggcg caccaagcga aaagctggca tatatcagat cgcggattga
     2041 agaggtattc tgagctatgc tcaagcctgc cgcaagtaaa ggctctgcta ttggcactgt
     2101 atccgctact ggaaaagcag cgagatcctt cgaagcttcg gacagaacca ttggcaactg
     2161 ataaatagcg ctaggtctac cggaaaggga ccaagatgca aattccttaa aagatgggat
     2221 tagtatggat tccagcgatt ccgatttcaa ttcatattgt attgtgactc tggggggcag
     2281 tataagagtg gcaaatatac ggttcggtac cttaacagcg atagtcttag gctcgaagcc
     2341 acgcttccgg atttcgattt ggtatgagcc tggcctaagc cttatcccat cagatgtatt
     2401 tccaatatat gtgttttcag cgtaaagggc tgctacatct ggattacctt tgaagcttat
     2461 cttggcgcca gggttaacaa gtcctgggtt gactaaaagg aaaaagagaa tgataaagat
     2521 cagcaccgca aagagaattg gtatgtatat acgtggccga atgccaaaga tcggctttaa
     2581 ggttacttta gccttttcaa tatcttgatc ggtgattttt tcgctctctt tcattataaa
     2641 agcagcctag ccgatgggca ccgtcttgtc aacgatatat gctcatgata gcttgaaatc
     2701 gggatgaaga atatggacat tctcgataag ctcgttgtgt tttctgaaga cctgctcaca
     2761 agaaggaaaa agagatctct ccgcgctaag atgaaacagc aaaaacgcca tcccattttg
     2821 gactggatat atgccatcct ttgggctgct tgtgttgtgc ttgtcataaa ccaatacttt
     2881 ttccaaaatt accgaatacc atcgggatcg atggaaaaaa cgcttctagt aggagatatg
     2941 atttttgtag acaaactctc ctatggtccc gaacttttgc ctggtgtctt aaaaacccca
     3001 ggagtctcca cacctaaacg aggaaacgtc attgtctttg aaaatcctac atatctctcg
     3061 cgcggcccga tctatacgat ttttcatcaa ttgctttata tggttacatt taccttggtc
     3121 gacatagaca gagagccaac cggcgagccg cgagtgcact atctcataaa gagagccata
     3181 ggggttgggg gagatacgct tcgcgtaagg aatggtgacg tattcataag gcctagggga
     3241 tcatcggtgt tcttttatga gcggatgttg atggatggtc ttggattacc tgcaaagatg
     3301 caacgattag ttgaatcaag cgaatatcca gagatagaca atgtcggcgt agcctcagcc
     3361 tatacagaac ttggcctttc tctcccttct cgagtaggga tgccaaatgt gcagcatgca
     3421 aataaggacg ctttccagta tgacatggtt cgtgtgacca ctctgcgaga tgcgaatccc
     3481 tctgactacc gaaacgcaca acttgcacag cgctatgaaa atggatggta tattgcggat
     3541 tcgcggctct ttcccatggg tgacaataga gacaattcac gcgatgcacg gtattttgga
     3601 ccagtcgctg aaaaaaaagt gctaggccat gctctgttta tttactttcc cctcagtaga
     3661 attggcagta tccattgaga gtgattattt atgttcgcta aacgctccat acgatatgca
     3721 gaaagaatac agcggaaaag gactcgaaac agaatactcc tatatatcgc tctttttatc
     3781 gttctgtgca ttgtcttgag gacctttgtg tttcagacct ggaaagttcg cggagagctt
     3841 atgaagccaa cgctcagaga tggcgacata gtcatagttt taccttgtct gtctttttcg
     3901 gataaaatgc tcgtcagttt ggctggcatc cctcaaaaag gcgatgtcgt tcttgttgaa
     3961 gacggctccg aatcgatcgt tcccactatg acgatgatcg aggattcagc attgagattt
     4021 atgactctgc agaaatattc acttctggca gccgaatttg gcaaaggctt cggtcttccg
     4081 acagtaatga gaattcagag tatccaacac gataaggata tttccaagtc acagcctgta
     4141 tataggcttg ctacggactg tcagagcgat aaaaacttca tgacaggcga ccgaattgtt
     4201 ccaccatcgc taattaaggg cagagtagtc ttgaagatat ggccattagc tcgaattgga
     4261 aaggtgcaat gaaagcatgg acttcacagg tttttgggca tgagagagga atatttccaa
     4321 ttatcgatcc attcaagata cgctcgctat atgtgcatat tccattctgt gcggatcgat
     4381 gttcttattg tgattttttc tctgtgcctc tgatcaaaga tccaactttc tctacaatcg
     4441 aatatgaaat ctcctatgtg caagcaacgc ttatgaatat tcggaattgg tcgaaactgt
     4501 ttggagcagg gcctttttcg acaatatacc tgggtggggg aacgccatcg gttattgata
     4561 gacgagcact caaaatactc atagacgacc ttgaacaatt tgtggataca gattgcgagt
     4621 ggaccatcga agctaatccc gatagtcttg ataaggcctt gctcgacatg ctcgccggaa
     4681 ctggagtgaa tcgtatctcc cttggaattc agactctctc agaagaagaa tggcctattt
     4741 tgcgacgagt aggttctata gatgaggcgg tgcaagccat agaactcctt tgcgagtatc
     4801 catttgagtt atcagtagat ctattgatgg gtattcccaa taagacaaac aaagggcggc
     4861 aaggccacag ttccattatg aactcacttg aatacctcat aggccgagtt tcacaccttt
     4921 ctatatatga cttaacactg gagaaaggga cattgattga aagccaagtc tcttctggaa
     4981 aattaattat gcccgattct gatgaattag ccgaagagcg tgataaggtc gatttatatt
     5041 tagaaggcca gggatttcat cgatatgagg tttcgaatta tgcgaaaccc ggtcatgaat
     5101 gcaggcataa tgccgtatat tggaatatgg agccctatct tggagtagga agtggaggag
     5161 tgagcacgat ccaatacccc atgtgtgaag aaagccagga gttgggcact attatcagaa
     5221 tcacagagac aagagacata agcgcatata tcgaagcccc aatggagatt cctaaagata
     5281 tagagataat cgataaaaaa accgctctgt tcgagtttct aatgatgggt ttccggacat
     5341 cgcgaggaat agatctaaaa agaattaaat ctctctttgg aatagacgtt gaggagtgca
     5401 taccttctgc cctcaagcga tggaatagcg aaatagtaag aaatggagat tttgttgccc
     5461 ttagaccgga caattttgat atactcaatc gatttctcgt tgaatgtcta gaagaactcg
     5521 atttatgagg ttttggtatg gctattttcc agtatgcagt gatttcatcg attttgcatg
     5581 acgagcctca gaaaaagcag ttgcttagtg ttttcgagga taaacttcag agattcggag
     5641 gcgaattaca cacgcttgaa tctaatatga ttcatccaag aaactctgag ctgccgcttt
     5701 tcatgctcgt actgacaggt ggaactgagt ctgaagccat gagcttgatt tctagagaaa
     5761 gaatgctcga tctcagaatg cccatagtgt tattggctca tccatcgcat aactctctgc
     5821 ccgcttgtct cgagattctt gcaaaggtta aacaagaggg tgggaaaggg ttgattcttc
     5881 aagccgattc ttcaggagca ttcgacgctg aagagctaga ggacataatt gccgtctgta
     5941 agagcagaat tacaatgcga agctgccaaa tcggtgtaat aggtagacct tcggactggc
     6001 tcatcgcctc gagtcaggat ttgcatgcag ctatgaactc atggggtgta aggcttgtcg
     6061 agattccttt agaagaactt gtgcggaata tcgaagagat acgctccagc tcctgtggag
     6121 tcccagtaat cgaggatttt caggaaaagg ccgaattcta tgctgaatcg agcaatgatg
     6181 atattctaaa gtcaatacag atattgcagg ccttaaggat tcttgtagat aaatatagac
     6241 tttcctcact tactctgcgc tgtttcgatc tcattctcaa agatcggagc acaggttgtc
     6301 ttgctctgtc ccagcttgct gatgagggga tcgatgcagg ttgcgaaggc gatattccat
     6361 cgatcatagg cttaagctgg atacgttctt tgacaggaca gattggatgg atggccaatc
     6421 catctcatat cattgcaaag aaaggagaag actcagccga aatgctgata gcgcactgca
     6481 cggcgcctcg ctcaattcta tccagatatg cgctgcgcag ccatttcgaa tcagggcttg
     6541 gggttgcaat atcaggaatc ctgcccaagg aagaagtgac acttttcaga atagggggga
     6601 agtatcttga taaactctgg tattctccgg ctcgaatcat agcaagtccg cgcgacgaag
     6661 gcttatgcag aacgcaagct ttgataaatc tccctaatga aaaagctcaa gagctgctta
     6721 taaatccgct ggggaatcac attgtcatgg ttagaggcaa ttggaccaga aggatttcga
     6781 tctctgcaca actttaggct ttcggctatt acctatacaa aaaagtcata gtttggtcga
     6841 atgcgctcgg cgaagccctc ctggaaagat atattagcgc ttttttccgt accaatggtg
     6901 cttggcggac catgaccggg aaaaataagg gtgctttccg gaagacaaaa tagtttttgc
     6961 ttaatgcggg caataagcgc attggtattg aaaacactat ttgtttttcc aataacgccc
     7021 gcatggagca catcgccggt aaaaagaaaa tacccttcca tgagaaatac aattgaatcc
     7081 tgagaatggc cgggcaccgc tattgcggtt atatcaaagc ctgcttcgct aaatgcatct
     7141 ccgtccctca gtttcctgca ctgaaatcca agcaattttg catcagaagc ataaatagtc
     7201 ggactataga tcttcatgat agtagataat ccattcacat gatgaatatg gtcatgggtg
     7261 acgagtatgc cgcagagatc gagctcgttt tgctcgattc tttcgatcat agtcggagtg
     7321 atctctgaag gatctactat tatagccttc tttaaagatt catttacaat tgtgtatata
     7381 ttcgaaaagc catagaccga ataatgatgg taaatcttca tagatagaga tgctccagat
     7441 ctcggacagc ctcagcaata ttcgatcttg agaagctgac attttgctct ttagaaggaa
     7501 tataaaaggc tctcttgaaa tcgcaattgt caatattgac agtattcatg tcgcacatta
     7561 tgaatcgaga gttatacaaa ttcgaacccg caaatgtaca atccactatg acagagccat
     7621 tgaagcatga ttggatgagt tcggaatttt caaagctaca gtccataaaa gaacatttcc
     7681 caaaggaaca aaaatctaca tcggcgccag aaaggtcaca agagaagaag ctagaactat
     7741 caaagaaaca taaatggaat gaagctcctg taaacagtga atgctgaatc cttgcgtttc
     7801 taaaagagca accaacgaaa cgatagcgag aaaaatcgag acccataaga aatgcattat
     7861 cgaagaccct attaagaaga atgtttttgc tttgcagctc ctgcatggcc ttcttcatgc
     7921 gctcaggata gtcctgcaag tgataatagc acaggttttc tagtgagaaa gcaggcaagc
     7981 tacagtcttc tattgagcaa gcagaaacct tgaacatgat tatagcctat atgatcctgc
     8041 gaggattctc aagactaaaa aaccaaagcc caaaagtaga tataagaggc tgcagctatt
     8101 cctattgatt tcaagcgctt gtcagcattt cgcttacaat gtagaatttt tgatatgtgc
     8161 tataaatgcg gttcttattt agcctatgat tataggccag gattcaatga tacctgtgat
     8221 atatgcggaa aagacctgca cgcgtgcctg atgtgtcgtt tttatacacc aggtgctcat
     8281 tgggactgcc gagaaacaat agaggagctt gtgcaagata aagaaaaacg aaatcattgt
     8341 gaatttttca ttatctctga gaaatatctc gaaaaagggg aaaccgcagg ttcaaagcag
     8401 gatgatgacg caaagagaag attcaatgcc ctgtttggtg aatagatatc taaggagaaa
     8461 tgaacggtgc gcatctgcgt atttgattca ggcatagggg gattgccttt cgtccaggct
     8521 gtgaatcggg agtttccctt cttagaaata gattatgtgg ctgatgatgc aggcttcccg
     8581 tatggaacaa agtcacctga gatgatccgc gatatactct ttgaacgatt taggagaatt
     8641 cgggcaagga ttgatcccga agtcctcata ctttcatgta tgacggctgt tcaaatagga
     8701 ctaaaggagc ttcaggcagc tcatagaaca ttacaaataa ttggcgccat acctcctatc
     8761 gcagaggcag cgaatgaatc gcagactcat cgaatagcgt tacttgccac ctctcgtact
     8821 accgaggatt ctttccttga ttatttgata gctagagatg ctcctgattg tgaagttatc
     8881 agaattcctg ctcaagatct gatcgaatat gcggaacaac gtcttccttt tgcatctaag
     8941 ctttccgcac agacaatggt agctccttat atcaattatg cgctcaagga aggaacagat
     9001 cgaattgtat tggccagcag ccaatttatc cttctcgaag attctattac taatcaattg
     9061 aatgaactac aagctttcaa tgtcaaatgt ctggatccga gggggaaagt gatttgctct
     9121 ctccaaaagc tcattcctga taaagagcat agtgaaggtc aattcgtaaa gccaagattc
     9181 tatctcaccg gggaccaagg tgccaaccct tctttaattg catggtcaga aagattcaat
     9241 tgttccaaac cacagcttct atgaatggca atagtaagga atccacggtt ttaggagttg
     9301 ttgtctcgtt tagcaatcga aatgcaaaag tcagatgcag tgatgggaat attttgacat
     9361 gctccctcaa aggcaaaata accgattcat cattccaagg ctacaaaagt cctgttgctg
     9421 gcgaccatgt atcggtaatc cccatatcga aacagaatgg agtcatagaa tccatttttg
     9481 agaggaaaag tgaattaagt cgctataacg aaaaagggga aacaaaacag gttttagcag
     9541 ctaatatcga ccttgttgta tgcatagcca gtacaagagc acctcctttt agacctcgat
     9601 ttatcgatag ggtgggtgtg atggctgaat attgtggtct gccttttcta atagtttgca
     9661 acaagatcga cttaggtctg gatgtttcta tcgaatctcg tctcgctgca ttccagaaac
     9721 tcggctatgg agtggtcaag agcagtgttc gggattctat aggaatcgat gaacttcgga
     9781 cgagcctcct tggaaaaact tcggtcttta tagggcaatc cggtactgga aaatcgagca
     9841 tcataaattg tctaattccg ggagcagatc agcgaatagc cgagatctcg gacaaacata
     9901 agagaggaaa gcacactaca accctctcag ccatggtttc ttcgtcaaac ggcgatattg
     9961 acataatcga tactccgggt attcgcaggc ttgcgatacg aaatatcgaa ccggatgatc
    10021 ttggattcta ttttcctgat atggttccat ttctggggct ctgtgagttt ggtgcttcct
    10081 gtacacatag atatgagttg cactgctttg tgaaacaagc cgtacaagaa gggatcatac
    10141 atcatgaccg ttatgagagc tatctcagaa tgcgtgcgga attagaagaa acaagaaata
    10201 caaagacaaa cgagtccagg aagccactca ggtctgctac atataaattc gaggatgaag
    10261 aatggtgact aatcagcata gccttgcgct cctagacttt tatcgactgc gtgatcgctt
    10321 ggaagaatac tgtttttctc acgaaggacg ggcgctcttg aatagtacct tgccctgttc
    10381 tcaggaaaat tcagtaaaga gacttaagaa ggacttgtcg ctgcttgttg aaggactcaa
    10441 cgaaaaagag attccttcgt ttccctttcc aaacatcgag aatcccataa taaaagcagc
    10501 caaagaaggc acggttctcg aagctgagga gcttttcgct attgggctct gggtaaaatc
    10561 ttatcatcag cttttttctt ttctgcggcg aattcttgac tcttttattg aagaatcaaa
    10621 agaagttagc gcagaaaact ccatggatgg cgagatccgg caagaagatt ccataaatga
    10681 cgaatcttgg gcagagtggg gaattgagaa tattgtgtat tcggcccctg ccttgagcca
    10741 agttacacag atgatatttg cagtcattac cgaagatggt gaaataaagg atttgccccg
    10801 gctaaaggct ctaaatctgg caattcaaag aacccaggcc gaaaatttgc acataatcaa
    10861 tcgatatctc tctgatcctt cgcttcaaga tgctttgcaa tcgaacatag ctaccgaacg
    10921 cgataatcga gtcgtaatag caatcaaagc taatttcaag ggaagaataa agggaatcgt
    10981 acacgaattt tcttcgacag gtcaaacaat ctttatagag cctattgaac ttgttgaacg
    11041 aaataataag cttcttgagc ttgagtctaa actcaatgaa gagataaggg ctattcttag
    11101 agaaacaacc gctgcactca ggcatttttt gcctatccta atgcgagcaa gaggattctt
    11161 ggctgccgcg gatgctcgct ttgcgcgggc tattcaggtc aagagagagg gtcttgctat
    11221 agcaggtcat cttgaacatg gtatacatct ttatcaagcc aaacacccct tacttgggaa
    11281 aaaggtcgtt cccatagatg tggatattcc agatgattca agaattctca taatcactgg
    11341 tcctaacaca ggaggaaaga cagtctctct aaaaacgatt ggccttcttg cgcttttgaa
    11401 tcagtatggt gccggcatac cagcattatc aacttcagca tttgcagttt ttgatacaat
    11461 tctcgcagat ataggcgacg aacaatccat cgaccagtca ttatctacat tttcaggaca
    11521 tatgaaagtt atttctgaga taatatcttc agctaccgat cgtagccttg tattgctgga
    11581 tgaattggga gcgggaacgg atcctgaaga aggttgtgca atagcaatgg ctctgctgga
    11641 ttatttatac cgattgcaat gtttcgcaat agtcagcacc catcatggag tccttaagaa
    11701 ttatggatat actaaacctg gctgcctgaa tgcctctatg gaattcgatg cagagagtct
    11761 tagcccgacg tacaagatga taatgggcgt tccgggagaa agcagggctc tcgaaattgc
    11821 tggccagatt ggcttgccaa tggaaattgt cgatatagcc aagagctatc tcgaagatga
    11881 gcgatcagat tattctatga ttattcattc tttgggagaa aaacagagag agctagagaa
    11941 gcttgaactg gaaaagaaaa agcaactagg gaaagtactt gagttacgcc gcaaggtcga
    12001 ccttgagaat ctcagaattc ggcgaagaga actggaactc agaagacagg gaattgctga
    12061 gctcaaacat tttattaatg aatctagaag gattttcgaa aatattgtgc gagagttgag
    12121 agaaaatggc aagaaagatg gggatacatc cgcaggacga gaattcttga aagaaatctc
    12181 agacgaaatt catcgacaaa gcgagacgct tgagagtttt gcacaagaaa ccaatagaat
    12241 tgaacgctcg ctaaaacctg aaatgcaagg caatatagga atagacatcg aaaaaatgaa
    12301 attcggagat accgtaatat tccacaatag agaagctgtt ttccttcgaa agcttgataa
    12361 aaagaatgca cttgtacaaa taggttctct tcgagtccca attgaagtcg aatctcttag
    12421 tcttcccaaa acgatggata gattcgataa agccgcaaag cctgactatc agattgaact
    12481 cgcttctgat tccatgggaa ggaatgcacc agtagccgaa ctcgatgtac gtggcatgag
    12541 actttcagag gctctagatt tgttagagca ccagatcgat gctgcaagtc tcgctggtgt
    12601 tagcagtttt tccataattc atggaaccgg agaaggtatt ctctctaggg gtattcatga
    12661 gtggttgaag aagcagattt cagttgcaga ttactatttt gccaggccag aagatggagg
    12721 ttttgggaaa acctggatcc atctaaaaga ataagatcaa gttcgctggt ctcaatgaat
    12781 tctcactgct tagtttctgg aaattgacat agcctgtgtt ttgctacctt tttttgtcaa
    12841 ttgacaccaa tttgtgcaac atgatatcgt tatttggttt ctcaaagcca aaggagtttt
    12901 catgtctgga catagcaaat gggcgagtat taagcataag aagggagcac ttgatgctaa
    12961 gagggggcag ctttttacaa agctgatcaa ggagatttcg attgcggctc gcatgggtgg
    13021 aggcgatatc gaaggtaatc cacgccttcg tacagcagta ctaaaagcta gggctgcgaa
    13081 catgcctaaa gacaatatcg atcgagccat aaaaaagggt accggcgaac ttgaaggttc
    13141 tatctatgaa gagctattct acgaagctta tacccagggc ggcggggcaa tgctaatcga
    13201 agttttgacc gataataaga atcgagcagc tgcagaaata cgaaatataa tcacgagatc
    13261 gggtgccaat ttaggaacta ccggatcggt cgcatacctt tttaagcgca agggcatcct
    13321 gacttatgat agcgaaaagt ataccgagga tcagataatg gaagctgccc tcgaggcagg
    13381 agcagatgat gtggtcagcg aggatgggac catttcagta tatactgatc cctcatcttt
    13441 tgaggatgta ttaaatgtta tgaacgacaa aggcttcgaa acactgggag cagaaatatc
    13501 aatgctgccc gactcttata taactgtcga tctggagaca gcccagaaaa tacaaaagct
    13561 catcgacaaa ctggaagaaa atgaagatgt acaaagcgtc tatcacaaca tcgagatgcc
    13621 cgagctcgag gaatgatcgg tgctttggat tctcgaagtt ctctagtagc tattggaatc
    13681 gatcctggca tagcagctgt tgggtatggc cttgtcagaa ctctggaagg aagactctct
    13741 ctggtaactt atggttgtat tgaaacggaa tcggacgaac ctattggtaa gagactgact
    13801 tatatatttg aagagattag gaaacttctt ggacaatacg ctcccgatat tggtggtatc
    13861 gaggagcttt atttttttag aaatgtctcc tcggcttttc ccgtggcaga agctcgaggc
    13921 gttattagac ttgctttcga aaagacaggc atagccctaa aggaatatag tccaaatgct
    13981 atcaaaaaag cggtgacagg ctcttcgaga gctagtaaaa gccaggttca ggagatggtc
    14041 aagatcctct taggtatgtc tcagatacct acccccgatc atgcttcgga tgctcttgcc
    14101 gcggctattt gtacattgca ttccattggt cctgcaggct atactctcta acatgtttaa
    14161 cagaataaag ggtgtattta gcggccacga ggatgaagcc cttcacatat caaccggtgc
    14221 cctggaatgg gaaatattag ttccaatccg ggatccgtcc ttttacaatc acacaggaga
    14281 agaaatagag ctttttacat ggttacatca ctacgaagac gcaatgcgct tatatggttt
    14341 tttttcggca ttggaacgca agctatttct agatctgcaa aaagttgaag ggattggtcc
    14401 tcgccaggcc ttcaagattt tgcagggtat agcgccgcgc tccctagtca ccatgttaga
    14461 tagtggtgat ctagcttctc tgcaaaaaat accgggcatc ggtccaaaga cagcccaaaa
    14521 aatgatgctc gcactaaaag gaaagttgat cactcaagag gaggcttctg ccgttatttc
    14581 cggttcccaa ggacgcttta gggatgtggt tcgcgctctg gttgagatgg gctttgaacg
    14641 aaaagaagtc gagcgcgtaa cagctcagct ggcttcatct ttagaagata gtcctgaagc
    14701 ggaaaaagaa ttgttcaaaa gggcgcttct tgagctgtcc gctggagctg gagcatgagt
    14761 ccttcaacaa tagataacgc atcttcatgc agtccaatga atcccctcta taatccggct
    14821 gaagatgaca aggaagcctc tttgcggccg cgttacctcg ctgaatttca gggtcaacag
    14881 aggattaaag acaatctgct catttttatc aaggctgcac gtgatagagg tgaagccctc
    14941 gatcatttat ttttaatggg acctcccggc cttggcaaga cgactctcgc tcttgtagta
    15001 gcatcggaaa tgggtgttga tttcaaacct acaagcgcgc ctgctattga gaagccaaaa
    15061 gatctggctg gcattttgac tagtcttaga cctggcggtg ttctgttcat cgatgaaatc
    15121 cataggctca agccatcaat tgaggagatg ctctacatag ctatggaaga ttttgagctc
    15181 gactggatta tcggacaagg ccctgcggct cgcacagtca ggattcctat tccacaattc
    15241 actcttgtag gagcgacgac caaggcaggc atggtatcgg ctccactttc aagccgtttt
    15301 ggaatatctc tgcgttttga ctattattct cctcaagaat tggcaagtgt tatccgccgt
    15361 tcttcgaata ttctcgatat aaaaatcaat gaagatgcta ttgaactgct ttcaggaact
    15421 agtagaggga caccgcgcat cgccaacaga ttgctgcggc gcatgcggga ttttgcccag
    15481 gtacgagggg aaggtattat cacgagagat atcactctag atgggcttga aagactcgag
    15541 gtggattatc ttggcctcga acgtcttgat cgagaaattc ttaagacaat aatcttgaag
    15601 tatggaggcg gtcctgtagg tgctgaaact ctcgctgtct ctgttagcga gagcattgag
    15661 agccttgaag attattacga accctattta attcagatag gtatgctagc ccgcacgcct
    15721 agaggaagag ttgcaactca aacagcttac cggcatctcg gaatagaaag acctaatgaa
    15781 aacggacagt ttcgattttg atctgccgga gcatttaatc gcacaatatc ccaccgagca
    15841 gcgcggtaga tcgcgccttt tggttttgga tcgcaaaacc ggaaacacca ttgattccat
    15901 ggtggaagac atagtttcct ttattgaacc aggtactctg atggtgttta acgactcgaa
    15961 agtgcgaaaa gccagactat acggaacatg tgaacagaca gggacaattg tagagtttgt
    16021 ctttctctcc ccatggaaac aggatgaccc aacaagcctt ggcgactcat catccgatca
    16081 tgaaagccaa ggcgaccttg taaaagcttg taagccgata tcaaccgtct cgcgactatg
    16141 gcgagccata tgcaataaat cgaaacagca gaagattggc cgttcgtata tgtttccagg
    16201 gcagctgaga ggttcaatta tcgaggatag gggacatgaa aaaacaattg aattttcttt
    16261 tatcgtgggc gatgaatatt ttgatctcta tggacatgtt cctctgcctc cttacataaa
    16321 acgagaagat acaagcgatg atgaactgcg atatcagact gtctatgcaa aacatactgg
    16381 ttctgccgca agccctacgg ctggattaca ttttacgcct caactacttg acgagctttc
    16441 tcgtaaaggg atcgaaatcg aataccttac cctccatatc ggattgggca cttttcaacc
    16501 cgtaagagct gataatgtcg aagatcacca aatgcatgaa gaatggttcg atatctcgct
    16561 cgaagctgca gataaaataa atctagcttt aagcgaagga agaaaagtac tagcagtagg
    16621 aaccacttct atgcgggctt tggaatcagc ctggtatggt ggaaaagtca agcatgggaa
    16681 tttttctacc aggattttta tctatccagg ttatgaattc catgtggtcg atagcctttt
    16741 taccaatttt catactccaa aatctaccct gctcatgttg gtgtctgctt ttgcaggaag
    16801 agaaaagatc ttggagtgct atcgtctggc catagaaaaa aattaccgct ttttcagcta
    16861 tggtgatgct atgctaattc tataatcctt tttcggaaat aatgaaaata tgccaaatct
    16921 attattcaag gcaattgaga actagattca gcactcaaag ccaagaaagg caaaaacgat
    16981 agattctatt tgccgctgta ccgattcgat atctttacta ccatcgatgc gctctatgcg
    17041 ccaaccttta ttggaaagac gataagccat actctcatac attgtctgca ctcttctttg
    17101 gaattcgagc gtctcataga tttctttctt taatcgagaa cgaatgcgag agatacagat
    17161 ttccggatct acttcaaata ggagcgtcaa gccaggggct ggaaaagcgc tattcaataa
    17221 ccaaggcaat ctatgtccgc atgttgttcc ttgataagcg agactagata gaacatatct
    17281 atctgagata accagtgaac cgctcttgag actttccatg atgccatttt taccatatag
    17341 atgctcattt cgatccgcag cgaacagatg cgcaactgtg gtgggaacaa ccatatcttc
    17401 accagagagg atggtgcgta tcagttggcc aatatgattc atggtgggtt ctgccgtaac
    17461 catacaggaa aatcctctag caatgagggt ttctcccagt aagcgaacct gggtcgttgt
    17521 tccggcgcca tcgattcctt cgatgacaat gaaacgattt ttgagctcgt ctgtgttata
    17581 tgtaggcata ttatgctagt atataggcga ctttagattt tgtcattcag ggttcgcatt
    17641 gaatgaggac gaaaaaatcc gtaatagtaa tcgtcatatt ttctcttgca ctcgccatct
    17701 ggggcagcat cgtgattgcc gatctagtgc agcgcgaagt gcagatgctg gccattaagg
    17761 ctttggatag gctacagtcg gcagttgtcg atgccactgg cctaatcctg aaatatgaca
    17821 atttagacat agagtctcta aaccgatttt ccatgcatgg cattgcgctt attcgttcag
    17881 atgctgcaga acataatggt aacaagcctt catcttattc ggagttctat ccttcctcag
    17941 aaagatcaaa ttatgaagaa caaccgccgc tgcgaatcaa aaaactatca atcaggataa
    18001 gcatttgggc tttattttca ggtcataaca atgaagccgt gagggatatc acggtagatg
    18061 atgtccagat ttctctttat ttgcccaatg atgaatacat cataaaaaag atactggaga
    18121 atttcgtttc aggaaaagca gaaagcgttc cacattttga actctcaatt gggccagtgc
    18181 gcgctgaagt gcgagagatt gcaaagagct ccaacgcttc tatggataat aatgtgatta
    18241 atgaaggaag cctgaccata gagtctctcc agttttcgag cctaactgat tctcctgtaa
    18301 tcatagcgcc ttcagtgatt ttgaagacta gtggtcttct tggtttaccg aaggacttga
    18361 atgcagaatt tggccttaga ggaagtggaa atgccaattt atccgatatc gatatgaaca
    18421 tagatcttga ggcaagttct acgggctggc acattatgcc tcaaaaactt ctgctggaga
    18481 agcgaggtaa gaggatagag tgtaagaagt caggaggggg tatagaatta tcgggtagtt
    18541 atgagggggg ggtatgggga atagagggtg tatttgaggg gtacggtgtt ggggaagaca
    18601 tagagttagg gggggagtgg ggggaaatag ttaaggggag tgaggtaagt ggcgagatag
    18661 gggtaggtgg tgatttagag ggattaagta gatatgaagt agatttaggt attgatatag
    18721 gggtaggcat aagtatagga ggatatgatt taggggcatt aggggtaagt ttaattggag
    18781 taggggatgc ggttagctat ataggtggta ttagggtaga ggtaggtggg aatgagttta
    18841 ggtatgaggg tgaggtaggc tatgagggga taaggctagg aggtaattat gagcttagtg
    18901 gagtggatat agggataggg ggttatataa gaggagggat aggggaatat gaggtaggag
    18961 taggtaaagg agagcttaag ggtgtggaga tagggttagg ggtatgtagg gttagggtag
    19021 gggtagaggg gtatgaggtg aggtatgagg gggcagtagg gggaggggaa gtgaaggtag
    19081 gaggtagtat aggcaaggct gggggttatg agggggtagt ggagttagaa ggggtaggaa
    19141 tatcagaggt gatgggtgcg ctaggggtag gagggataga gggtgaggtg agtggtcgag
    19201 tatatggttt agagagggga ggtaagaggt cttggagtat aagtgggtta gcatatgatg
    19261 gagaggtgat gggtgcagag gttagtttaa gaggggatgg ggtaggggat gaggcagggt
    19321 atgagataaa ggtgttagag gcgagtatag gagattatga tatagctttg aggggtaagg
    19381 gtagctatgt tggggaatta tttagtgggg aattcagtgc tgggggtatg agttatgagg
    19441 tgagtgtaga ggcaagaggg ggtaaggtaa gggtaggcat aggggagggt tttagagggg
    19501 catttgagct agtgggaggg cggttagagg gggaggtagg gatggataaa tttgggttag
    19561 ggttaggtga ggggatgata agggtaggag ggttagcgag tgggtggtat gaggctgggg
    19621 gatggggaat aagtttagag gggatgagtt taagctatga gggggagggt aagtatccgg
    19681 aagtaaaggt tagtgggttg ataggggata gtggtatacg atttgagtgg ctagaggtga
    19741 agggtgatgg gtatgggttt aaggggtcaa tagatctgaa gggtgagggt ggtgagtata
    19801 tattaattgg gagtggcaag ggagaggggg tattagggga aggatctgag tactcagtat
    19861 atggggtaat aagaggtaag ggagtagatt tgagggtagg agtacaagga ttaggggtgg
    19921 gtagttatgt ggtagaaggt tatgctgatt tgagagggga ggtggacata agggaggtat
    19981 tgaagggttc tttggaaggt ataggggaca taagaggtga agtagcggta ggtttgagtg
    20041 gttctgggtt aagggtagat gagcaacgat ttgaggtgga gaagcgaggt aagaggatag
    20101 agtgtaagaa gtcaggaggg ggtatagaat tatcgggtag ttatgagggg ggggtatggg
    20161 gaatagaggg tgtatttgag gggtacggtg ttggggaaga catagagtta gggggggagt
    20221 ggggggaaat agttaagggg agtgaggtaa gtggcgagat aggggtaggt ggtgatttag
    20281 agggattaag tagatatgaa gtagatttag gtattgatat aggggtaggc ataagtatag
    20341 gaggatatga tttaggggca ttaggggtaa gtttaattgg agtaggggat gcggttagct
    20401 atataggtgg tattagggta gaggtaggtg ggaatgagtt taggtatgag ggtgaggtag
    20461 gctatgaggg gataaggcta ggaggtaatt atgagcttag tggagtggat atagggatag
    20521 ggggttatat aagaggaggg ataggggaat atgaggtagg agtaggtaaa ggagagctta
    20581 agggtgtgga gatagggtta ggggtatgta gggttagggt aggggtagag gggtatgagg
    20641 tgaggtatga gggggcagta gggggagggg aagtgaaggt aggaggtagt ataggcaagg
    20701 ctgggggtta tgagggggta gtggagttag aaggggtagg aatatcagag gtgatgggtg
    20761 cgctaggggt aggagggata gagggtgagg tgagtggtcg agtatatggt ttagagaggg
    20821 gaggtaagag gtcttggagt ataagtgggt tagcatatga tggagaggtg atgggtgcag
    20881 aggttagttt aagaggggat ggggtagggg atgaggcagg gtatgagata aaggtgttag
    20941 aggcgagtat aggagattat gatatagctt tgaggggtaa gggtagctat gttggggaat
    21001 tatttagtgg ggaattcagt gctgggggta tgagttatga ggtgagtgta gaggcaagag
    21061 ggggtaaggt aagggtaggc ataggggagg gttttagagg ggcatttgag ctagtgggag
    21121 ggcggttaga gggggaggta gggatggata aatttgggtt agggttaggt gaggggatga
    21181 taagggtagg agggttagcg agtgggtggt atgaggctgg gggatgggga ataagtttag
    21241 aggggatgag tttaagttat gagggggagg gtaagtatcc ggaagtaaag gttagtgggt
    21301 tgatagggga gcaaggagca gatctcgatg tagattattt acaatatgca ggacaatcgc
    21361 taaagggctc aatgattatt caatattcct cgatagctaa tctattaaag gatacaaaga
    21421 tagattattc attcttggac attaagactg atagaacaat tctggaagga gggattgtag
    21481 ccttaggtga taaactagaa gtcgcgatta agacatcatc gattccattg gaagaagtat
    21541 ttccctctac tttcaatatt tctggagata tagaactaaa gggaagttca accatttcaa
    21601 tattgagtaa aaagctagat ctgtctgccc tgtcttctct aaacttgcag ttcaacacga
    21661 gaaaatcaga aatgaaagga atccctttct cagcagcagg tacaataaat ttcaaagatt
    21721 gcactttttc tttagaatct ggaatcttct cttatcagaa ctacagattt gaaaatatca
    21781 atgctgaata caatctagaa tcaaaacaag tcgcttatgc gctaaattcc aaaatagtga
    21841 ttggagaaca attgctgagc gccaacctga agggcttcgg agacaacata gatagctcta
    21901 ttttcaagct cggtgctgcc caagccttcc atattaccgg agagataaat gatgccagct
    21961 taggatctaa gaccatagat ccacttagct attctatttc caatgcagat gataagcttg
    22021 atattcatct aagccaagca aatggggata gtatcgatgc atccataact aatatgacag
    22081 actttgatct tgttttaaat gatctattca aaataaaagg tagagccaat ggcaaaatcc
    22141 aagccaagga agtatgggcc gatattgctt tggagaaaat tgatctaaaa acgttaggag
    22201 ccttgatctc aaaagatgat ctaggagata cttcaggcat tgcttcagcc gaactaaatc
    22261 tctccggaga tatatcagac cccagaattg agggcaagat tctattcagc aatctgatta
    22321 tcgatcaaaa tctttatatc gtagaaaaaa tcgggccatt cgatgcagaa attgctataa
    22381 atgatggggt agcggaatta agtcctacaa ttatcaacat tggaacaggc gatatctctc
    22441 ttgctgcgac tgcgaacttg gctagatggc aactcagcga tatcacggtt ttagtctcga
    22501 ctctcgagaa agcttcaatg cgcttcaaag gtaaaatcgg tggattgaat actgatggca
    22561 tatggattaa aggagatata aaagcggcaa tatcctcgaa ttccatcgaa attggcggcg
    22621 acatgcttct tgatgacggg acactagaag tcgatcctaa tggttttatt tcctccgaaa
    22681 atgcgcaaac tccagtctca cctctctatc tcaaattaat ggttacattt ggaaagaatg
    22741 tccaaatgta tatacctggc aaggatatat cccttatcag aggaacaata tctcctgctt
    22801 cgacttttac ggtccagtac gatcaggcga gaggcgattt tgcggtaaat ggtaggattg
    22861 agctgcgctc cggatacgta tattatcttc tcagagattt ctttataaaa aaatgcacaa
    22921 tagaattagc ggaaagtcag tccaaattta atccagtcat ggatgtcatt gctgagttga
    22981 tggaacctac caaggatgga atgatcaaga taattctttc agctgacaaa tctcccttag
    23041 ataacttcag tcccgtactt tcttcggtgc ctccaaaaag ccaaatcgag cttcttgcgc
    23101 ttcttggtgg tggattggca ttgtcggaat tagcggaaga cactcctctt acattgaggg
    23161 aagccgtaat cgcaagttcc gagtttctta cacataattt gatctttaga tcattcgaac
    23221 aacgcataca gaaggcttta ggcctcgatg ttctctacct gcagtcttcc tttatccagc
    23281 gatggcttct cgatattacg gaccagaccc gtgagaatca gacgaattta tctagatatc
    23341 taattggcac agagcttttt ggaggaaaat atataaccga ttcagctttt gtgcattttt
    23401 ctcttatgat agagcaggac ccgcttgaga aaactggatt tttgcaatta aatccaaaag
    23461 ttgggcttga actgcaatct ccatttggtc ttttgcaatg gagtatgtcc tttggtaagg
    23521 aaggaatgcc gctaaataat caagagctca gcttgagctg gcgcataaat ttttgagtaa
    23581 ctacaggagc agcaatgaga tattcgcaca agcatttcat cgtaatcata atgatatttt
    23641 gttcttttca aataattctc gctcagacct caacacaaga gcttgctggt caagattatt
    23701 ctcaggattg gttttggaat aagaaaatcg ctggatttag atgggatgga ttacactatg
    23761 cgaaccgcaa tgatctagat tcaatgctgc gcgattacat cggcaccctt tttacagaga
    23821 atatttggat agagattcaa gcaaaactct atgcacttga ttggtttgag agcatcgaat
    23881 cgatggcgtt gccggctgga gaggaagaag ataaaataat aatcaaattc atagtaatcg
    23941 aaaagccatc tattaactca ataagggtta ttggaaatag ttctatcagg acctcggaat
    24001 tgctcgacgt tgcttcttcg aaaaccggag cgatttttaa tgaagataaa gcaaaagccg
    24061 attcgcttgc gatgcaaaaa ctctatcttc agaaaggttt tccagatgca agagtggaat
    24121 atgaaaagac tgaggttgaa ggagactcca atttaatcat attgacattt aaggtaaccg
    24181 aaggaacagc ggttgttgta aaaaagatcg cattctcagg tatttccgca ttttcttctc
    24241 agactctcaa gggccaggta acgcttaaag aagctgggct ttttcagaaa ggagcattcg
    24301 aagaatcaaa gctggctgaa agtcggacag ctttagagga ttattatgcg tctaaaggat
    24361 atatagatgc caaagttata gatgttatta agaattattc aaaagatgaa aaaagtcaga
    24421 aaaactattt agatatcact tttgttatag ccgaaggcaa gcaatggaaa ttaggtgata
    24481 tcttctttga aggcaatagc atattttcta catctcgcct agcttcgcta atatctctta
    24541 aacagaacga acccataaat ctaaagcgac ttctcaccga caagcaaaaa atagacgatc
    24601 tatattatga atctggatac attttcaaca ctataacaat gaccgaaatt cgagatgagc
    24661 aggggggaag aatctcattc aagatctcga taacggaaag ggacagggct catattgaga
    24721 gtatttcctt caagggcaat acaaagacaa aggactatgt tattgcaaga gaattaccac
    24781 tcgaagcagg cgaagtattc tcaaaagcca aaatcatcga tggattgcga aatctataca
    24841 gccttcaata cttctcatcg atcgagcctg agattcatca aggaagcgtc gttgatctga
    24901 tggatttagt cataaacgtt gaggagatga gtacggctca aatccagttc ggtcttaccc
    24961 tctcggggct cggtcagcca tctgctttcc ctatctcggg attcataaag tttagcgatt
    25021 tgaatcttgg aggcaccggg aagaaccttc aggtcaatat gactctttct ccagatgaac
    25081 agagtcttga gacttcgttc ggtcagagct ggcttttcaa taagcgcata tctcagagca
    25141 ttagcttcgg tatagaacat tcagtccttc agactgcgca ggattgcatt gctcctattt
    25201 tctctgagga ggatattccg gatcctttta ttggactcgg aagcggatcg gacgaatggg
    25261 atggcctgct ttcgtccgtt ccagatgcat acttgatgga ttacgatgat tattctttta
    25321 gtcttgggta ttccatggga tatgttcata aaataccgca gggcgatgta ggaattgccg
    25381 ggggtgtttc ttcgggcctg gacatgtctt cctacgatac cgccaagtat cgtccatacg
    25441 aaaaagaaat gcgcgactat aatggtcaat gggtattgaa ggattctatt tttggccgtg
    25501 tatatttgaa tcgtctggac tactggtata atcccggaaa gggatacttt ttgagccaac
    25561 gtcttaccta tacgggcatt cttccaatgg agcgtcaatt ctatattaga tccgacacac
    25621 gcgccgagtt ttatgcaaca ctattctcta ttcctttttc tagttcctgg atattctcac
    25681 ctgttatcgg gattcacagt ggagttcagg cgcttttgcc tcaaccatgg aattcgctag
    25741 cagttacgaa agactgggta tatattgacg gcactttcaa tgtgagaggc tggaaatctc
    25801 tatacaaagg gcacggaatc cttgtatggg aaaactggct ggagctccat cttcctattg
    25861 ttccgcaggt tctcagcttt gacggatttc tcgacgcagg cgcaatgatg actgagaatg
    25921 gctggctaga tatgactctt gattcgccgg tttccaaagg gagtaatgcc ttagaatgga
    25981 ataattttgc tttttccatt ggttttggag ctcgatttat gattcctcag tttcctttta
    26041 gattctatct tgcgaagcgt ttcgtatatg atggttccaa agttgaatgg aagaccagag
    26101 aaggttcctt cgattttgtt ttgagcatta cacagcctct atattgattc atctcaaatt
    26161 tgtatagagc caaagacaat aaggctgctt cttgtgctat tagaaccagc cttctataat
    26221 ggtgccatgt caaaaaatcc agactcaatg ctcgatcaat atcgcagaat aaaggcaaag
    26281 catcgcgatg aaatactttt ttttagattg ggcgattttt acgaaatgtt caatgaagac
    26341 gccaaagaag ctagtgccat tttaggtctt actctcacca agcggcaagg ccttcctatg
    26401 tgtggaattc cttatcatgc cgctaaaacc tatatcgctc gactactcaa agccgggaaa
    26461 aagatagcaa tttgcgaaca gaaatcgctt tccaataagc gcggtctttt ggatcgtgaa
    26521 gtagtagaag ttattacgcc aggtactgcc ttagaagacg attatttgga acaggggaca
    26581 aataattatc tggcagatat atgtataatt gatcagaggc tttgttttgc ttacctagat
    26641 gtatccacag gtgaatttag agccttttct ctgacttcaa ataaagaaat ggtaacggag
    26701 actattcgag cagaactata tcggctggca cctcgcgaga tcctggttca acaatccatt
    26761 cttgatcaac ccaagtttta tcaggtcatc cgtgaatgca gcgcgatcat tgaagcccaa
    26821 ccagactggg tgtatgatct tgtccactcg ctcgacctgc ttaagaaaaa attcggtgtt
    26881 gtatcactga agggttttgg ctttgaagat aatgctcctg aaatcgcagc agcaggcaat
    26941 ttacttgaat atgtgcagga aatgatacgc caagattgtc ctcatatccg tgccttgttg
    27001 ccctatgaag aaagagaatt cgtgctcttg gatgaggcta caaagagaaa cctggaattg
    27061 atgaggaatc ttagcgattc tagtaagaga gatactctta tcgaagttat agataaaacc
    27121 aaaacagcgg ggggagcccg tcttctgcgg caatggctct ttcaacctct caggattcgc
    27181 gaaaaaatag aggcgcgctt agaggcgatt tcctttttgt atcataatca aatattgctg
    27241 gcagatatac gaaagctgct ttatggaatc ctcgatatag agagattgat atcgcgtctt
    27301 gctgtcgata aggctcatgc gagagatcta agggctattc gtgattcaat cgatttaggt
    27361 ctggatcttt ttactcttat agaatctgct aaagcgcctt tagagcttgg tccaaggatg
    27421 agcatggagc aaattgacgc atgcaggcaa gttatcgata tcatagaaaa gactatcttg
    27481 gatgatccat caattctgtt gaccgaagga aatctcataa gagatggcta tgacaaggag
    27541 ctagataatc tgaggaatct gcataaaaac actcaggaaa ttcttgaaga ctatcttaaa
    27601 gatgagaaaa ggcaaacagg tattcaaaat ctacggataa gatataacaa aataatcggc
    27661 tattacattg aagtgacaaa aggaaacatt gctaatgttc cttctcactt cgttcgcaga
    27721 caatcgttgg ttgggggaga gcgatatact accgatcgat tagcagaact tgagagcaaa
    27781 atcaatgggg cacaggagag aataatagaa attgagaaga atgtttttct caaaacaaga
    27841 gatagcctga agcaatttat cccagcacta ctagaatgcg cttcagaaat atcaagaata
    27901 gactgcttgg catcacttgc acagactgcg acagagcaag gctttacaaa acccctaatt
    27961 actgaagatg aagatataga gatagtaaat ggccgacatc cggtagtaga aatatgtctg
    28021 ccttatggcg attttgtacc gaattcaatc gccctttctc gaaagaataa ctggtttgta
    28081 ttaataacag gtcctaatat ggctggcaaa tctaccgtgc ttcggcaaac agccctcatt
    28141 gtgcttcttg ctcacatggg cagttttgtg cctgctgaat cagcaaagat agggctggta
    28201 gataagattt tttgtcgtgt cggagcccag gacaatctag ccagagggga gagcactttt
    28261 ctggttgaaa tgcacgaaac agcatttatc ctcaatactg cgaccgagaa aagccttgta
    28321 atcatggatg aagtgggccg aggaacaggg actctggatg gagtttcgat agcttgggct
    28381 gtaagtgagt atcttacaag tagaatcagc tgtagaacac tctttgcaac gcattatcat
    28441 gaacttacaa ccatggcgat tcctggtatc cgaaacatga gaatggtagc aatcgaacag
    28501 gatggtaaca tatcttttcc aaaaaggctc gaggatggtg cttcaagcgg ctcttatggt
    28561 atacatgtcg ccaagcttgc tggattgcca aaagaagttg tcataagagc atcgcaggtt
    28621 gaatcacatt ttaatgctct agagcaagga ctaaaagaag aaaaacccaa agaagactct
    28681 accccttctt tggagctgtt tagtaacgag gatattgtca tcgagacatt gaggaacata
    28741 gatccagaca atattactcc aattaaggct ctccaaatcc tgtccgaatt ggttgaaaga
    28801 ttgaagcaat aatattaaaa aagaacgaat atttgataat gtactcgatc ttcagaaaga
    28861 ctaataatcg gactctcgat gctatatttc cgcagcattg cgtactatgt ggatcaattg
    28921 ttggagcggt accttggttt cctgcaccat tgtgtgcctc ttgtgaaaga atcctagagt
    28981 ttatcagtgg tccgcgctgc aagcactgcg gaagagagct catcagcgag aatgaattct
    29041 gtatggactg ccgcaatgcg cagactttgt tggctggcat aattccagta ttcagttttc
    29101 gtggcaatgc agccagtctt attcataagt ttaaaacagg ccagcgcatt tccttggctc
    29161 actattttgc ttatcgaatt tcgatgctcg atgaattgac agcagatgtt gctagttcta
    29221 catgccttgt acctgtgccg ccaaggcctg aaaagctgag atctggtcaa cttgatcaag
    29281 taggtgcgat tgcagaatca ctcataaatt ttggttttca ttatgaaaaa atgcttttaa
    29341 gactgccagg aagcagtcag caaaaaatgc tcgatcgtgc tggccgcctc aaaaatgctt
    29401 cacaatctta tgcgctctta tcaaacaaaa aaaccatagc aaagatcatt ttgctggatg
    29461 atgtttgcac gacaggcgca actcttgaag cttgtgcaaa actattgatg gaaaacggaa
    29521 acaaagttgt gagtgctata gtccttgctg ctgattaggc ttatcgcgca agagcgaatc
    29581 ttgacgaatg ctaagagaat atttataatg acactataaa aaagtctgag agattctttt
    29641 ttgcggcatg caggaagagt cgtgcaaacg gctctttttt attccctgta ttttggggga
    29701 aatagagcgg ctattgttga acacttaaaa agtgtttaaa gattgctggc ctgcaagtag
    29761 gaaatacgcc aaaagcggtt tctctctgga tatgcgcttc cgcttgagct taacaatcgt
    29821 tgacatagaa gcacatcgcc aaaagaaggc aagataaata ggcctgtctg gctggagtct
    29881 gaacgactat taagcctagc attatgcgga agaaaaaagg cggatggaag gggatgatag
    29941 ataaggaact tcaagaagag atcgatgcaa cactaaaagc aggagggctc gatctattgg
    30001 aattctcatt gactcggcaa aagggtggtc ttcgtgtcaa agcagtgatc tatagacccg
    30061 aaggaacagg caccgatgaa tgcgccagag catatcgcct tatcctgccg cgtattcaga
    30121 tggcgttggg agtgcaagat ccatatatcg aagtctatag cccgggcata gacagggtct
    30181 ttcgcacaga gagggaatgg atagccttta agaattgtta tataaaattc atgacttccg
    30241 atagtcacga ctggaagaaa ggccgcattc ttcattttga taacggaaaa gttcaaatag
    30301 aaatggatga tacaatattg aatattccta tagcttcgat tataaaggca aagcttgatt
    30361 catctcgtaa gggagaaaaa gtaaatggca tctgaaatgg cagaagcaat tcatcagttg
    30421 atctctgaaa aaggaattcc tgaggatctt atagttaaga cgctcgaaga ggcgattctt
    30481 gcagcctaca agaaaaaatt cggaaccatc gataatgcta tagtccgaat gaaggaaaat
    30541 tatgaaggga tcgaaatcta tgcaaaaaag actatagtct cggatgaaga ttttgaggat
    30601 gaagtcttgg agattcctct tgaggaagcc aagaagttcg cagacgaagc cgaaatcgga
    30661 gatattctag aaatctcaat cgatccatct gagttcgatt ttcaatcggt tatgcttgca
    30721 aaacagacag ctcgacagag cctgagggat atttctcgcg acactctata tgcagagttt
    30781 aaatcgaaag taggagaaat agtcattggc tatttccagc gagagcgaaa cgggacgata
    30841 tttgttgatt taggcaagat tgaaggtatt ttcccgcgga agtaccagtc tccaagagaa
    30901 acatatcatg tgggcgatag aattaaagcc ttaattgttg aagtggaaaa gaccaagact
    30961 ggatttcagg ttgtcctttc aaggacccat gcagaatttg tgcgcaagtt gctggaactg
    31021 gagataccgg agatctacga caagaccatt gaaatattta aaattgtccg ggagcccggc
    31081 tatagaacga aagtagccgt atatgcgaaa cgaagcgatg tggatccggt aggtgcttgt
    31141 gtaggtccaa agggcatgcg gagtcaactg atcagccaag agcttgaggg tgaaaaaatc
    31201 gactttattc gatatgatat aaacccaaaa gagtttatta agaatgctct tgcgcccgca
    31261 caggtaaaag aagtatatat actggacgaa gccaaacaca tggcgctagc cgtagtcgat
    31321 gagcatgaac tttccatcgc tattggaaag tcagggcaga atgtcaagct agcaaatcgg
    31381 ctatgtgact ggaatataga tgtcatgaca gaggaacagt atttgcagga ctccagaaat
    31441 atcgagttaa aaaaggccgc agaccgtcta ttttctgtag aagaggaaga aggtggagaa
    31501 ttcttaatta gagatttgcc taatctagag caaacttgga tagatgctct ggaatcagca
    31561 ggcattcgct tgatagagca gttcctggaa ttggatgaag aagagattgc acggaacact
    31621 gctctgagcg ctacagacat cagcgcaatt cgtgacataa tcgaagataa tgtggaaatc
    31681 atacaggaag aggtagaaga agaaggaatc gtgtcttata gttgtccgga atgtggatca
    31741 ccaataacta tagacatgga tcattgccca tcctgtggag tagccctgag ctttgaagca
    31801 gaagaagagc aggatgaata aaggaagaat atgacagaga gcaacgataa agaccttaag
    31861 aaggtgcatt taatcaaaca acctaggcaa gatagtcccg agcagcctca ggaaatgtcg
    31921 gctcagaatc ctctcgatca gggcgcagaa aaaaagaagg ttgttgtggt ggtaaagaaa
    31981 aaaacaatca ccacaaagaa agttcaggca aaagcaatga tcccaggaac tcctaaagat
    32041 caaaaaatcg atgaagaaca atccgcattg tcaacacagc cgcattccga agtaggacaa
    32101 aataaagcgc agatagcaaa agaggatcaa gtcgcatctc cttctccgat aacgccctcg
    32161 acaacgacgc ttccatcatc caaatcacca tcaaatgttc catcaatgaa attgtccgaa
    32221 cacaagcagg aaatagggca agatgagaca attatcagct ctcagcggcg gtactcttcg
    32281 gatgggcgcg caggaaatct tgctcaaggc agacctagtt ctacatttcg gaatagtcat
    32341 gatagaggaa gttcgcgtcc tgctaacgga tcttaccaag gcagagcata tggtcagcca
    32401 tctggaagga gctatccatc atcatccagc cgatccggac aatccggatc tcagcagttt
    32461 tctcgtggaa atcgtccttc tggtggcttt cagagtcaag gtggaacagg ctctggtggt
    32521 aggacagcat atccttatgc aaaaacaggt cagcctggaa gcggccaaag gccgggtgga
    32581 tatattggag caagacaggg ccctgcaggc ggcagatacg gattacgtca aacccccgca
    32641 ccggttcctt ccactgggaa cgaaaggccc gcttcgcgta gagcaccggt caaaaagaaa
    32701 ggagtattca acaagcgcga ggaagaaacg gagctcgaga agcaaattca gctcaaaaaa
    32761 aaggccgaag ccaaactggc agctgttccg aaatcaattg acatcatgga gaatatcagc
    32821 atttcagagc tggcaaagaa aatgaatcta aaacctgctg acctgataag taaattgatg
    32881 tcacttggcg ttatggcgac tatcaatcaa aaaatagacg ccgatacagc cgcaattcta
    32941 gctgctgaat atagctgcga agtcaaagta gtaagcctct atgatgagac agtaatcgaa
    33001 aaagcagtgg atagaccaga agaactcagg ccaagacctc caatcgtgac tgtaatggga
    33061 catgtcgacc atggaaaaac caaactcctg gatgcgattc gcaaaacgga tgtagtgtct
    33121 caagaatttg gtggtattac tcagcacatc ggtgcatata tggctgaata ccctaatgga
    33181 aagatatctt ttcttgatac tccaggccat gctgcattca caaaaatgcg agctcgagga
    33241 gcaaatatca cagatatcgt agtactagta gtttcagcta cagaaggcgt catgatgcag
    33301 accaaggagg cgatagatca tgcaaaggct gcaaatgttc caattattgt tgcgattaac
    33361 aaaatcgatc tgccggaagc caacccagac agagtaaaaa cgcagctctc tgaattgggc
    33421 cttattcctg aagaatgggg aggtcagaca cagttcgtcg aagtctccgc tctccaaaag
    33481 ataggcatta gggaactctt tgatgcaatt ctcctgcaag ctgaaatgct cgacttaaaa
    33541 gcaaattacg acaggctcgc tgaaggcaag atcattgaat cccgtataga ccaaggccgt
    33601 ggaattgtcg ccacacttat ggtacaaaat ggaactttgc atgtcgggga tccttttgtt
    33661 gcgggtattt tcccaggtcg agtcagagcc atgttcgatg ataaaggaaa tcgcatagaa
    33721 caggctacgc cttcgatgcc agtagaggta atcgggttcg agggcatgcc cgaagctggc
    33781 gatccattcg aagtggtgga agatgagaaa tttgcgcgtc aaatctcaac caagcgccaa
    33841 gaattgaaga aatacgaaga aggccgcaat gtcaaaaaag tgactctgga taatctctac
    33901 gagaccataa gccagggaga gatcaaagag ctgaaggtca taataaaggg agatgttcat
    33961 ggtagcgtag aagctctaaa aggcatgttg gagaaactat caacgagcga ggtgcattta
    34021 aatgttatta gagctgcggc aggcgctatt acagaagacg acgtcatgat ggcttcggcg
    34081 tctaatgcta tcattgttgg ctttaatgtc cgcccaacat caagtgcaaa gttgc
//
LOCUS       DAOMZL010000008        33082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_1006_length_33082_cov_15.174433, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000008 DAOMZL010000000
VERSION     DAOMZL010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 33082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33082
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1006_length_33082_cov_15.174433"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..2793)
                     /locus_tag="PLQ30_01430"
     CDS             complement(67..2793)
                     /locus_tag="PLQ30_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HPW01137.1"
                     /translation="MNIEFIDPLRDIGEGLLKVQKPARYTGGECDAFPPIDPLDSRLR
                     IALSFPDLYEIGMSNNALRIIYAMLNERRDALVCERVFAPARDFEHLLIDKHIPLYTL
                     ESGIPLKETDVLGFSIGYELLATNILAIIDRAWIPLENIERGEGDPIIIAGGPAITNP
                     MPFARFLDAVWIGEAEEGFTELMLKLAELKSRGATRIEKLHLLALHPSIWISPFLQEL
                     GLQPKKKVRRAIYQDFYRTEFVPSFPYPVINPVHSHGSVEIMRGCPNGCRFCHAGYFY
                     RPQRCKSPSIIKKEIEDLIIKKGYTEITLSSLSSGDYPDIVGLFQDLTSSWSKHNVSF
                     QLPSLKVDSFTLPLIEKISEVRKSGLTFAIETPLDSWQCGINKRVPFEKVKSILHEAK
                     LKGFRSAKFYFMIGLPVAERGVEEAEEIISYLNNVASIEHIAIHVNIGTFVPKPHTPF
                     EREGQLSENESLKIIKYMRKKLRSTRNIEISYHSPFLSVLEGLISRGDESIGEIILQA
                     YKQGARLDAWEENLDRNIWNNALLNYNSKKGEGAWEKFLAKRAENEDLPWEEISLRIS
                     SRWLKNEKKKAENNQLTLICNENCTNSCGVCGYENSVVSNMILSKPDAESEDSIIIIG
                     KAKAIEDIDNGSEKIDKSRLIGIIDDLFKDNEDDKSRVRSIVAIKLIGVWRKKDIAVL
                     YPMHDVARAFFRAFKVSGFSAAYTQGYNPQPKFELSPPLPLGVEGENEIIAVWINVPE
                     ENIQKACQFICDYQKQLLELININLPKGLHMNALKPSKFLQQRKKTIGSMLKATKWKY
                     RFGNDSSFSMAVDLLKKYSDFFIPINLDSSSISSSNEIELLEKFPGSAGKSLSLFKIL
                     KENSYEFCLQYQDSDIGSSLADSSMKCFKAKRTGCYGYDENKNSYELLQNMI"
     gene            complement(2790..4109)
                     /gene="rodA"
                     /locus_tag="PLQ30_01435"
     CDS             complement(2790..4109)
                     /gene="rodA"
                     /locus_tag="PLQ30_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPW01138.1"
                     /translation="MKFQNFKRLFGDIDYYLLFSCFLLCALGIIAIYSSGIDAEGAII
                     SNEYLKQILWFGIGIFLLIAAALFDFSRYKDIVWVFFAFAILLLIITLLAGRVVNGAR
                     SWLGIGGIGIQPAEFTKVATVLMLARYLESAEFDSSFKKLLITGIIMIIPLALILAQP
                     DFGSALVFVPAALVMLTLADVDFRYILFGVLSIFGVFLFLSLPLYASNHPNPENIIYT
                     AITHENVLIAILIFVLVISIISGIGWLKFKKRYYYWFSYSMAILAISMIGAVAAHSIL
                     KPYQIMRLLVFIDPSIDPKGSGWNILQSITAIGSGGFLGKGFLQGTHSHARYIPQQST
                     DFIFSIIAEEFGFIGCTVLFILYGILFFRCVFLIETCKDRFSQFVIGGLLGIFAFHFM
                     INIGMAMGIMPVTGIPLYFVSYGGSSLWTGLISIGILTGISARRYSA"
     gene            complement(4106..5965)
                     /gene="mrdA"
                     /locus_tag="PLQ30_01440"
     CDS             complement(4106..5965)
                     /gene="mrdA"
                     /locus_tag="PLQ30_01440"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPW01139.1"
                     /translation="MAMLPKKITLRMRILKALLISLLSVYSINLFSMQILRKELFVSE
                     AQRISIQSTRIPAPRGEIYDRSGRDLLAGNREAYSVYITPSELPDSKKSDVIKKLSLL
                     LGIPTEDIESKFPDLSLYSYGRVVVAKNASLEAVTQIAARIDEFPGVSWSSKPLRNYA
                     DLRSLSNVIGYVGAITRDEYKLLYNKGYILEDLTGKAGIELEHEELLKGKDGWISKAV
                     DVYGKDISSNAAEIQEPIPGKRLILSIDGNIQRIAEEALGSREGSIIVLKPSTGEILA
                     MVTYPYYDSRVFMSDNIGKAYIDLLNDPKKPMLNRAYQSSYPVASTFKTVLTAGILEE
                     GSISPDKKIYCSGELIYGGRSWSCHIKRPGHGALDLKNALAQSCDIYFWIAGRDYLGI
                     ENIVSYAQDFGYGKATGIDLPAENAGLLPTPTWKEEKYNDTWTHGDTMNLSIGQGYLL
                     ASPLQLANMMAMIVNDGIIYKPHILKEVRDPTTSALIDEIQPEILHQSSISAKTFETL
                     KSYLRDVIVHGTARVPINTKVVQVAGKTGTAEVGLKDRWHSWFVSFGPYDAPAKDQIV
                     VTTMIEASNPWEWWAPYAANVIYQAIYANQNAHDAAKAVGVRLEGSSLIGRRE"
     gene            complement(5994..6503)
                     /gene="mreD"
                     /locus_tag="PLQ30_01445"
     CDS             complement(5994..6503)
                     /gene="mreD"
                     /locus_tag="PLQ30_01445"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPW01140.1"
                     /translation="MKSIFISGIVATLMLFIQSTWLSRGIIMGIIPDLALGVLLFCSF
                     INREGQGIIASFLVGIAADILSASPLGYYAFLYTSCGYFATLISYVTEKDIFIVPFLL
                     GAGATIIMALLSKVIYLLFSAGIHYYHIFSMEFGIELMLNGLFTILLFFLLSFIRQLF
                     EHNPKKAMP"
     gene            complement(6500..7378)
                     /gene="mreC"
                     /locus_tag="PLQ30_01450"
     CDS             complement(6500..7378)
                     /gene="mreC"
                     /locus_tag="PLQ30_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPW01141.1"
                     /translation="MKTGSQIHGPARAMHKKAGICLVLSIIVLFISTKTIVKVPGFFN
                     GYIIGGIQKGLSAIGSFFTGTIGAVSELRNIERDYEALLEKVQDYELKVRDYTALREE
                     NQRLKELLGLSTLPDAKKIAAHVIARDPTNMYSSLVIDKGYINGIRKYMPVVAYQNGV
                     EGLVGKIIEVKASTSILQPLYDQRFFAAARLAKTRAEGMLNGQGFRDVPLNLLYVPKA
                     EADLLKRGDIVVTSGLDEIFPSEIIIGRVEDWRLNELSSSLVISVQPAIDLSRIEYVF
                     AIDIEPLSSPKKEAIQ"
     gene            complement(7395..8414)
                     /locus_tag="PLQ30_01455"
     CDS             complement(7395..8414)
                     /locus_tag="PLQ30_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPW01142.1"
                     /translation="MFSPDIGIDLGTCNTLIYMNGKGIVLDEPSVVAVERGTNRIVAV
                     GTEAKSMLSRTPTDIIAKRPLRDGVIADPDMTEKMIKYFIQRVFPKNLFFKPRIVVGV
                     PTCITKVEENAVIDSTYKAGARMVKVIAESLAAAIGAGIPITEPAGHMICDIGGGTTE
                     ISVISLKGMVVTNAIRVGGDEFDEAIAKHIRNIHNLIIGEQTAERLKIEIGNANPERT
                     IEKIEIKGTDAITGLPRRFEVDSVEIREALMDSVNLIVEEIKKTLGKTPPELASDIVE
                     RGIVLSGGGALLKGLPKLIAKETGVPVILSEHPLECVAIGAGKYFEIMQKNIGSSSVY
                     ESLNL"
     gene            complement(8446..9387)
                     /locus_tag="PLQ30_01460"
     CDS             complement(8446..9387)
                     /locus_tag="PLQ30_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW01143.1"
                     /translation="MLDRFFRGKAHHTKKKNPWLFIAIALIAVGVFLGFYFAIRGRSA
                     KNSISRSALAKVWNSGNNIEQTLSEAQKAIESYPFDEYYLSICGIAYYYSALNAQEEE
                     IRQQELEEAVIALRKALAVGLPSRMKGQVYYILAKAYYQKGEPWFDLAERYFLLAKES
                     GSKEKDIPQYLAIVYAGRKDYSRAVSWFELALENNTSDALLLSAAISYMNTDQIEKAR
                     DLLLRLESSASDAKIRLKATLLLAQDNFNSGDIQSAQDRYQGVLKEDPVNVDAWYGLG
                     LIYAKLNDQLGARAAFRKVVQIDPNHADARKRLAEKL"
     gene            complement(9404..9816)
                     /gene="rnpB"
                     /locus_tag="PLQ30_01465"
     ncRNA           complement(9404..9816)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLQ30_01465"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(9825..10748)
                     /locus_tag="PLQ30_01470"
     CDS             complement(9825..10748)
                     /locus_tag="PLQ30_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPW01144.1"
                     /translation="MPMRMDDLFETLREYQELLSNRVELEKQLEAMPKTIEIQNQMLS
                     REKKSLVDAKEQFTTTEANLKQLRQALAEAEMRRENAERQMDGIQTQREYDAVNKEIR
                     EASEKEQDLRHRIQEEERRFAEIDDRLHRIEISVSALESEIEQKRAEIDQNSKEIIEE
                     IENLRTKENSLTQELEDDLMFKFERIIRSKSGMGIVPIHGVVCTGCHMILPANFVNEV
                     RQADKIVFCPYCSRILYYEETEIEGSIEEILTGSLVDLGDEDSEDADDAESEDESEDT
                     SDSSKKSSSKKRKNQTEPLIDNDFLIDNMEE"
     gene            complement(10761..12488)
                     /gene="rpoD"
                     /locus_tag="PLQ30_01475"
     CDS             complement(10761..12488)
                     /gene="rpoD"
                     /locus_tag="PLQ30_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HPW01145.1"
                     /translation="MNDMELDPAIAKLLEYAKEKKTISFDELSDMLPEQILNSDKMEA
                     IINLLVNNDVKIEEEVQIEEEALPKLTSIPLKSKMVSSEKEISSDDPVRLYLRDIGRE
                     NLLTAEQEVELSRMMEQGENIIKNTLRRSGMLIPEFHQIVVKATRKEPKDMSLQKKEN
                     TEKIAERRRLNQFYRELIDSSAADLRFYIESKKNIIAKGGNIFEDPHLIRIREPLLII
                     VNETDIHPEEITIFSEKFIVAARKIIRYRKEQDRIQGILQVSSSKDLRTLGRNLTIKE
                     KREEIEQRLGLSAEEIKDKIRQLQVNEKKLQDIENNFETPIDDIISMAREVNRGRKMM
                     KSAKDRLIKANLRLVVSIAKKYTNRGMQFFDLVQEGNIGLIKAVEKFEYRKGYKFSTY
                     ATWWIRQAITRSISDQARTIRVPVHMIEQINKVVRESRQLMQKLGREPTDEEIASQLG
                     WEIERVKAVKNVAREPVSLDTPIGEDEDSLLQDFIEDKEVENPAVQTNYNLLQEQLRM
                     VLSTLPKREQEVLRMRFGLDEGYSLTLEEVGLYFNVTRERIRQIEAKALKKLRQFKRS
                     QKLRDYIDH"
     gene            complement(12481..14271)
                     /gene="dnaG"
                     /locus_tag="PLQ30_01480"
     CDS             complement(12481..14271)
                     /gene="dnaG"
                     /locus_tag="PLQ30_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPW01146.1"
                     /translation="MARISPQIRQEILDKTDFLSVYQEHVRLQKKGASFWGLCPFHSE
                     KTPSFSVSPDTGLFYCFGCHKGGSIIQFIMDIDKLSYTEAMQELAQRCGVQIQFEESE
                     KLGETEKERQTLLELNERLSKSFHWFLTNHDCGRIALEILHRRGLSDQLIERFSIGYA
                     PANRQWLYSFLKAKGYSDDFLKHSGLFSKKDLSYPLFADRIIFPIGDAKGRIIAFGGR
                     AMTEDGPKYINSSETAIFRKQETLFGFSQAIDSIKNTDNAIVCEGYMDALSFHAAGLT
                     NAVAPLGTAFTSQQAVVIRRRSSNIILCFDTDEAGLRAAERSCSIATAAGLETRVLLL
                     EEGKDASEILEKRGAGELTRMAKNTINAGQFLLARANQLFDISTMEGKAKAVSFLFPF
                     LDALDSDVKRQEYIKEIGRSIGVSTHAVAMDYAKAKVSGVRRVRDSYNLSNVPYSSSN
                     SARTSDLLFMAAVILSEDPFQFVDENLNIENLDDPRAKDLFSALQEAHKEGILDVEGI
                     LSLCTDEAAARFVREVDTSGELQGNLEKIIKDGLDQKKKAALERKRRELVARLKSFSA
                     DTDPLQEKLILEEIIKVDGELKTTGGDIDE"
     gene            complement(14278..15294)
                     /gene="mltG"
                     /locus_tag="PLQ30_01485"
     CDS             complement(14278..15294)
                     /gene="mltG"
                     /locus_tag="PLQ30_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPW01147.1"
                     /translation="MKANHIRSIMQNLLILFLILLLAIVILILTPPFRSESVEVKIKS
                     GSSLRDIAKELHNNHVIPSSRVFELYVRARGSERNIKSGTYRFMPLELPIVAANKLIK
                     GNYITQKVVIPEGRTARQIALILESNNVTRAEAFLEAVNDIELARSLGIPASNCEGYL
                     FPDTYFFDPQSDSRELVSQMVRGFFAAIERIEGSKTEILNPQSLHQRVILASIIEREY
                     RLAEDAPLIASVFLNRLRKNMPLQSCATVVYVMTEHLGKEAPSVVHYGDLKIQDPYNT
                     YINRGLPPGPISNPGEISLKAALFPPRTDYLYFRLEDINTGKHRFSHTLEEHNYSEII
                     PKGL"
     gene            complement(15294..15734)
                     /locus_tag="PLQ30_01490"
     CDS             complement(15294..15734)
                     /locus_tag="PLQ30_01490"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPW01148.1"
                     /translation="MRCNACCSGQPGFVWLSKRDLMALSEYFDLSIQRFALTFCRIVD
                     LGIVTTLSLKEKQNNDCIFLENDGCSVYPVRPVQCRTYPFWETILENEQSWIEEGKQC
                     PGIGRGSLISSDRILDAFFERRQNAPLSIDEIAALAALRNNWDI"
     gene            complement(15876..15958)
                     /locus_tag="PLQ30_01495"
     tRNA            complement(15876..15958)
                     /locus_tag="PLQ30_01495"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15922..15924),aa:Tyr,seq:gta)
     gene            complement(15982..16054)
                     /locus_tag="PLQ30_01500"
     tRNA            complement(15982..16054)
                     /locus_tag="PLQ30_01500"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16019..16021),aa:Thr,seq:tgt)
     gene            complement(16148..16909)
                     /gene="surE"
                     /locus_tag="PLQ30_01505"
     CDS             complement(16148..16909)
                     /gene="surE"
                     /locus_tag="PLQ30_01505"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPW01149.1"
                     /translation="MRILLTNDDGIQSDGLFALIDTIAAMKDFNGKSLHQVWVVAPEH
                     ERSGVSHAMTLKKPAKLRRLGDSRYSCSGTPVDCIIIAGLAILHEKPDLVISGINRGP
                     NLGTDIVYSGTCGAARQAALQGIPAIAISCAAHCDNLEYEGLTSFLSANLKELVALWK
                     PDSFININGPSTKSRDLKAVWTVPGKNKYIDNLKCFDGSDGYTYCFLTEGAEERMPDA
                     LSDHHAISQGYIAVGLVEVHPRAFHEPSLNGQFVF"
     gene            complement(16948..18096)
                     /locus_tag="PLQ30_01510"
     CDS             complement(16948..18096)
                     /locus_tag="PLQ30_01510"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HPW01150.1"
                     /translation="MKDIFIMHHAEYDTAPDCTSAAPTIVKILGEHTDFADGLVLSAS
                     LSFEVRVAISFRKDNSLRFYAADFNERKRANVATFKYKREDRWANHVKSVCDYFWRVF
                     NLDPRGLNVTIQSNVPLGLGLGISSAINMATALDLKTLYDLDLKLEELAFHAWKAQSL
                     FFEKELPLTNYLGITAPAGRSFSIIDLHDKKRRGVQFLPERWEMLLTDSKVPRSSVDA
                     ELEQRIADCKACLSVLVPYHNRSIRDIAPQELDELLGIVPERKRRRCLHVLEEVRRVI
                     EAEESLSRQDYIGFGRIVNKSHASLRSLYEISCPEIDWLTKRALELDDVLCSRLVGQG
                     FGGSTLTILNNEKKQAYRNKLEDYERIFGFKPIIHEVSTGAGLRILEH"
     gene            18569..19390
                     /gene="amrB"
                     /locus_tag="PLQ30_01515"
     CDS             18569..19390
                     /gene="amrB"
                     /locus_tag="PLQ30_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPW01151.1"
                     /translation="MSEKKIARSPLLSGIFYPSEPQMLMSEIDGLSLYAKRIPCPAFG
                     IIAPHGSFLYSGKISMESWGAILGSNPSIIVIMGPSHLPYEDGVFLPESTRFDIPGAC
                     LNIDLNLRDYLLNKIPMLKKDDLPHLEEHSIELQLPFASHFFPGVPILPCIISGKNPL
                     TIETATRLFEEISRYIGNYAVFVLSSNLAVSDSPEECDLLSKDFIYSLSRPVAEFTGN
                     VRACYNSFCGEAVIRAFRIAFPEARAELLDYDNSSKHREYCDELVVGYGAIGFKR"
     gene            19394..19972
                     /gene="amrA"
                     /locus_tag="PLQ30_01520"
     CDS             19394..19972
                     /gene="amrA"
                     /locus_tag="PLQ30_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPW01152.1"
                     /translation="MEFAISPEDQKKLLNYARLTIHASLKGEKEIDPPHLSTSLKCGA
                     FVTLKKNGSLRGCIGRIQGDANLPSTIKQMALAAAFEDPRFPPLHEEELPLIEIEITL
                     LSPLYPISPEEIVIGKHGLLIYANGRSGILLPQVPIEFGWDKETFLEQLCWKAGLPSN
                     IWKSPLARLYGFEGFVFSESELKEASSSDTCP"
     gene            complement(19900..21159)
                     /locus_tag="PLQ30_01525"
     CDS             complement(19900..21159)
                     /locus_tag="PLQ30_01525"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HPW01153.1"
                     /translation="MDVLPRIEISPILSAIDCLPECCDQGFLQSKFWGLFKSRTGWQA
                     YSCTYSFYNKDLSGRILVLKRKFAKLFSFLYVPFGAAELSSIPERWITLAALGRALGE
                     SFGGRDVFIRFDLPWECDVDPAKTKEEKKPDIENSLGFHISGLYKGTDVQVPDTVILD
                     LRDEEQRILAKMKPKWRYNIRLSQKKGISVTDEGFSSLPLFMSLYKETARRDRIAIHP
                     ESYYKTLFETSSEISEVAQENSITTTRPRLSLYVARHKDEVLACIIVLHFGNISTYLY
                     GASSSEKRNLMPTYALQWQAILDAKRFGADKYDFFGIPPDDKDASHPMAGLYLFKTGF
                     GGEIIHRYGAYDISLRPFLYFAFRTAEKMRSFWHKRIKKSLNKKTVGLSGKATKESTI
                     RNVHQGQVSEELASLSSDSENTKPSNP"
     gene            complement(21198..23447)
                     /gene="metG"
                     /locus_tag="PLQ30_01530"
     CDS             complement(21198..23447)
                     /gene="metG"
                     /locus_tag="PLQ30_01530"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPW01154.1"
                     /translation="MKRRLITSALPYVNNVPHLGNLIQVLSADVFARFCRLSGYETLY
                     VCGTDEYGTATETKALEEKVSPRELCDRFYAIHRDIYEWFEISFDTFGRTSTPEHTEI
                     TQSIFLDLDNNGYISSQSIDQLYCDTCGRFLADRYVRGTCPHCGYDGARGDQCEMCGK
                     LLDPMELLNPKCISCGGTPRPRSTRHLYLDLPAILPKLEAWMKDASVKGFWANNAIQM
                     TQAWIRDGLHPRAITRDLKWGIPVPKEGFKDKVFYVWFDAPIGYISMTARLAKERGFD
                     WKSWWQKPDEVELFQFIGKDNIPFHTVIFPSSLLGTGKKWTMLHHMSSTEYLNYEAGK
                     FSKSKGIGVFGNDAMNTGIPADIWRFYVFWNRPETSDHTFTWADFQEKVNGELIGNLG
                     NLVNRTLTFISRYYKGIIPDGTPDEAFWKEVTDREQETSKLLELADLRDAFRTIFSIA
                     DLANKRFQYEEPWKARIDNPQKASSLMRDLCYVLKDLAIMTYPYMPRTAEKLASFLGI
                     EIGHGKLGWADLGLRGDLKRIEKHEVLFNRLEDELIASLRAQYSGSQKEREEMAEQNR
                     KSEGQLPVSEEEDHNDRLDQIIDLCVARIIKIERHPKADRLYIETLDDGSGLERVIVS
                     GLVPFYREDELLGKNIIIVNNLKPAKLRGIESKGMLLAASTKTPEGNDIVEVLTAPKA
                     NPGERVLPEDIDMENPAETVPKESIDIETFLRIPMYAKDGYAWIKGQRLMAHGEPIRL
                     EKVMDGKIG"
     gene            23526..23804
                     /locus_tag="PLQ30_01535"
     CDS             23526..23804
                     /locus_tag="PLQ30_01535"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HPW01155.1"
                     /translation="MLRLVLKLNETSTIKDKRRVVSGIKDRLQHRYRLSCAETDLLDS
                     LVFAEIGAALVSNSAQYGEKVLQDAVTFIEDHYPVELFEVQMHYEIYG"
     gene            23818..24462
                     /locus_tag="PLQ30_01540"
     CDS             23818..24462
                     /locus_tag="PLQ30_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01156.1"
                     /translation="MKSLGKILLFMAFTISLTLCFISCIGIEASTKIDSKGAGTFSVV
                     YQISKDFVDFGSEETTPSLPLALSREDIERGLQGSEGISLKSYSKTNNANNTIISFAL
                     NFASPENLINYLDPTRSYTRFSEENGSYSLKFDFGNDIPPLDPDMKKYIFEQFSSYRF
                     ILTIETAKSPPPEVSIKYGDFIRKTSSGKKTILECAMADLLTVSSSPQIEIVWR"
     gene            24635..27421
                     /gene="ppdK"
                     /locus_tag="PLQ30_01545"
     CDS             24635..27421
                     /gene="ppdK"
                     /locus_tag="PLQ30_01545"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPW01157.1"
                     /translation="MPRRKFVYAFGAGLAEGDGSMKELLGGKGAGLHEMTKIGLPVPA
                     GFTITTEVCELYYKNGKKWPEGLEAEIQKHIKNLEKVTKKTLGDPRDPLLVSVRSGAP
                     VSMPGMMETILNLGLTDASVEGLASKTGNRRFALDAYRRFIMMYSSIAMGIDREIFDK
                     AFEEIKETKTRPRIGIPSEQKVNDTDVNEEELGELIKSFKKIYRDHIGTDFPQDSWEQ
                     LKGAINAVFDSWMAEKAVTYRKVEKLVGIKGTAVNIVQMVFGNKGDSSGTGVCFTRDP
                     NTGENIFYGDYLINAQGEDVVAGIRTPIKLAELEKLDPKVYKQLVDIRRKLERHYRDM
                     QDLEFTVEEGKLYMLQCRTGKRSPIAAFRIAVDLVKEKLITKEEAILRIKASDIEGIF
                     YPMIDKSNTTALKRSFLVQGIDAVPGAAVGRVVFNAKVAEEWEEKGEKIILVRKETSP
                     EDVGGMHAAQGILTATGGKTSHAAVVARGWGKCCIVGCEKLEIDYENRHFAVGDKIVK
                     EGDYITLDGNTGNVYLGQLKLVKPEPPKAYKTLMSWVDRTRKLKVRTNADTPYDAEKA
                     RELGAEGIGLCRTEHMFFDSEERILAIREMIIADDTESRKKALAKLLPFQTKDFEGIF
                     RVMDGLPVTIRLIDPPLHEFVPHDEEGQRELADAIGVPIEKVIQRVEQLHELNPMLGH
                     RGCRLTITYPEILDMQVTAIITAACNMTKNGIKVLPEIMIPLVIDDKELKILESRARE
                     IAAAIIAASEVPLKYMIGTMIEVPRAALLADRIAKVAEFFSFGTNDLTQMTLGLSRDD
                     AGRFLPDYIDEKKGAIFKADPFQNIDQDGVGMLIRMGIEKGRSSRPRLKVGICGEHGG
                     DPESVKFCHRVGMNYVSSSPFRVPVSRLAAAQAVIEDAQAAHKSTRKPGRPPKAASSK
                     AKRPRGRPRGSKNK"
     gene            complement(27512..27973)
                     /gene="dtd"
                     /locus_tag="PLQ30_01550"
     CDS             complement(27512..27973)
                     /gene="dtd"
                     /locus_tag="PLQ30_01550"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPW01158.1"
                     /translation="MRAVIQRVSEASVTYRTSREEKFCGSITKGILIYLGIASDDSDD
                     DAEYLADKIAHLRIFMDSNDKMNLSILDLGYEALVISQFTLHADVRKGRRPSYSKAAG
                     SEKALMLFDLFCTKLLENGVHIQTGKFQEIMRIHSINDGPVTILLDSKKAF"
     gene            complement(27988..29052)
                     /locus_tag="PLQ30_01555"
     CDS             complement(27988..29052)
                     /locus_tag="PLQ30_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01159.1"
                     /translation="MKKPVCFFILLFIVIGALFAIDIRNGLVRIATDDLTLQPALYRL
                     VNISGKSKYEPLWFSGDPRTSFIVLNIDNRIYRMGASKEYKTSQRQIKNGIEIEYRSV
                     TNRLTQRLLFFTSPFSRVADGFVIEIEIENYTTREMKVMIKEVCDTVLGEKEGFHFAT
                     SLNARVTDEMILASDSDEKYVVSPGQAASLALLLDEDQKPDMVVIANWKRLSDSKFYY
                     DSQLMKGFTLSSFSINDSALGLYWNERIIPPKGSIKVASTWLTGGSGSEFIDWLSLNY
                     PFPRQEPQQVLESPPVVLEEQSIVNAPILDIKAIQDLISEIDRAIDNIENLTDNELQD
                     IMTKLNLLEQSEGLAAPQSN"
     gene            complement(29049..29903)
                     /locus_tag="PLQ30_01560"
     CDS             complement(29049..29903)
                     /locus_tag="PLQ30_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW01160.1"
                     /translation="MKKLIMKMFAVIMFILASFSYAQANKTSDEFSYAFDIFRNGDFG
                     RAGAAFADICQTESNMEILADSCFMAALSYFQAADFPTSYEFCEKFMQSFPSHPNMPD
                     MQFLQGRILFKLGLYADAISVFDIFINRYPDSPFCSSVLFWKGESYYQLGDFAQALSI
                     FNKIKEAWPNTEATSLTAWRITVMASESREAKYARLAEFESRRNIYRDVQQMQEDVYG
                     EQRHLRDYLWSKAIRKYSARPNIETRELSADETSRNNRLNMLLEAKERALNLLIWKID
                     NYLKDITP"
     gene            complement(29988..30668)
                     /gene="rpe"
                     /locus_tag="PLQ30_01565"
     CDS             complement(29988..30668)
                     /gene="rpe"
                     /locus_tag="PLQ30_01565"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPW01161.1"
                     /translation="MKEPIIAPSILSADFYDIGSAIRHVEESGCPWLHLDIMDGHFVP
                     PITFGHKMIADIRAKTELFLDAHLMIEEPERHINAYIESGADAITFHLEACVHSHRLI
                     QSISRAGIKAGISIVPSTPVSNIVPLLEFLDLVLVMTVNPGYGGQKLIPFCLQKVEEL
                     RRIREQRGLHFLIAIDGGVNAKSISDIVIQQPDVLVIGNAFFNAQDRKQFVADFRNAW
                     TSPIGNID"
     gene            30825..31781
                     /locus_tag="PLQ30_01570"
     CDS             30825..31781
                     /locus_tag="PLQ30_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01162.1"
                     /translation="MAHQPRPKEEATIAEENLYLAYESLKNADIQSADSFLENALKAD
                     FEHPEVLFAMKCLHFWKDLISQMPETGSLLERGDFLCGSWKKFILFLSHIGDSFDTAR
                     YVYRRFIFGLALDQYLAIPDEEKEAFGPLLDLRLGRARKATGDIETALIHLERAAQAR
                     RNDANILAELADVYAMSGEARLSKALFREAFFIDAQAIEIDFLESELIFKIIENIQKL
                     GYSGSDIAEWIPVYAEIWGVFNIKRELSGAEYNRISASARQLEIQLRESHQQRSSLIP
                     RLLKKYFWMADYLKAREDTKELHSVLLKIKIIDQSIYESYIG"
     gene            31795..>33082
                     /locus_tag="PLQ30_01575"
     CDS             31795..>33082
                     /locus_tag="PLQ30_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPW01163.1"
                     /translation="MDQSPLSEKLQLMLNEEKWTRTLMANYSIANLKELDRFIEEITA
                     QNVADDIIKLCIEHLSQTKNSVIALYVAGMLNIMRQPADETYLIQLMDLFAESKKSNI
                     VEYICNRMLDFGENRIALVRLAECYTAENKIEQRYTVWESLVRIDTEEAELAQLIAEW
                     KEKNGLIEEAIDFYKKALIRYINKGLFSNIKELWHKLIQLSPEDYDFFLHAQKKIAKQ
                     VSPEKAATILMDLYDWYKKNEKWDTALNILKLIVEYDERNPALRKEFLECYSKIYSGH
                     SMFDECVRLSTLTQSYRSLHETIPDFEKRIAFDRGNYVFHRTWNIGRISNITNDEVVI
                     DFAKSRNHHMSLKMAFDSLITLSKDHIWVLKATWPRDKLREKVKTDPAWTIKIIIKSF
                     DNRADMKRIKAELVPSILSISEWNSWSTRAKEILKTD"
BASE COUNT         9339 a         7307 c         6650 g         9786 t
ORIGIN      
        1 tatattctgt ctaccaattt gcttcaagga ttattcgatg atctatggga gccagggcac
       61 aactatttag atcatattct gaagaagctc atagctattc ttgttttcgt cataaccata
      121 gcagccggtt ctcttggcct tgaaacattt catagaagaa tcagccaaag atgatccaat
      181 atcggaatct tgatattgca aacaaaattc ataggaattt tctttcagaa ttttaaaaag
      241 gctaagagat ttaccagcac ttcctggaaa tttctctaat aattcaattt cgttgctaga
      301 tgagatgctg gagctatcga gatttattgg tataaagaag tcggagtatt tctttaataa
      361 atcaactgcc attgaaaaag atgaatcatt tccaaagcga tatttccatt tggtagcctt
      421 aagcatggag cctattgttt ttttcctctg ttgaaggaat ttgctaggct ttaacgcatt
      481 catatgaaga ccttttggca aattaatatt gattaattcg agcaattgct tctgataatc
      541 gcaaataaac tggcaggcct tttggatgtt ttcttctggt acatttatcc agacagcaat
      601 gatctcgttt tcgccttcaa caccaagagg taaaggcgga gacagctcaa attttggttg
      661 aggattgtag ccttgagtgt aagcagccga aaaaccagat actttgaatg ctctaaaaaa
      721 agccctagct acatcatgca tgggatataa tactgctata tcttttttcc tccagacacc
      781 tataagcttg attgcgacaa tcgatcttac tctcgacttg tcatcttcgt tatctttgaa
      841 taggtcatcg ataatgccaa tcagcctgct tttatcaatt ttttctgagc cattgtcaat
      901 atcttcaata gccttcgctt ttccaattat aataatcgaa tcttcactct ccgcatcagg
      961 tttagatagt atcatattag atactactga attctcatag ccacagacac cgcaactatt
     1021 tgtacaattt tcattacaaa ttaaagttaa ttgattgttt tctgctttct tcttttcgtt
     1081 ctttagccat cttgaggaga ttcttaacga aatctcttcc catggcagat cttcattttc
     1141 ggcccttttt gctaagaatt tctcccaagc accctcccct ttttttgaat tatagttcaa
     1201 aagcgcatta ttccaaatat tgcgatctaa attctcttcc catgcgtcca gtctagcacc
     1261 ttgtttatat gcttgcagaa taatctcacc tatagattcg tctcctctag aaatcaaacc
     1321 ttcgagaacc gatagaaaag gtgaatgata gcttatttca atgtttctgg tagacctaag
     1381 cttttttcgc atatatttga ttattttaag agactcattt tctgataatt gtccttctct
     1441 ttcaaaaggg gtatgaggtt ttggaacgaa agttccaata ttaacatgta ttgcaatatg
     1501 ctcaatactt gcaacattat tcagataaga aatgatttct tctgcttctt ccacgcctct
     1561 ttcagccact ggaagaccaa tcatgaaata gaattttgcc gatctaaaac ctttgagctt
     1621 ggcttcatga agtatggact tgactttttc aaaagggact cgcttattaa tgccgcattg
     1681 ccatgaatca agaggcgttt ctattgcaaa ggtaagacct gattttctaa cttcggatat
     1741 tttttctatc agtggcaggg tgaaactatc cacctttaga cttggaagct gaaaagatac
     1801 attatgcttg gaccaggaag aggtaaggtc ctggaagagt cctacaatat caggatagtc
     1861 accagaagaa agagaagaaa gtgtaatctc agtatatcct ttttttataa ttaaatcctc
     1921 tatttccttt tttattattg aaggagattt gcatcgctgg gggcggtaga aatatccggc
     1981 atggcaaaaa cgacagccat tggggcagcc tctcattatc tcgacagaac catgagagtg
     2041 aactggattt attactgggt atggaaagct gggtacgaat tcggttctat agaagtcttg
     2101 gtatatggct cgcctcacct ttttcttcgg ttgtaatccc agttcctgaa gaaaaggaga
     2161 tatccatatt gaaggatgta aagccaataa atgcaacttt tcaattcgag tcgctcccct
     2221 gctctttaac tcagctagct taagcatgag ctctgtaaat ccttcttcag cttcaccaat
     2281 ccatactgca tccaaaaatc gtgcaaaagg cattggattt gtaattgccg gtcctccagc
     2341 aattataatg ggatctccct cgcctctctc tatattctcc agaggaatcc aagctctatc
     2401 aataattgca agaatattag tggcaagtag ctcgtatcca atactaaatc ccaatacatc
     2461 tgtttcctta agtgggattc cgctttcaag cgtgtaaaga ggaatgtgtt tatctataag
     2521 caaatgttcg aaatcccgag caggagcaaa gactctttca caaacgagag catcccttcg
     2581 ttcattgagc atcgcataga taattctaag agcgttgttc gacattccaa tctcatatag
     2641 atctggaaaa gaaagggcaa ttctcagtcg tgaatccaaa gggtctattg gtggaaaagc
     2701 atcgcactct cctccagtat agcgagctgg tttttgtact ttaagaagcc cttctccgat
     2761 atctctcaga ggatcgataa actcaatatt catgcggaat acctgcgcgc agaaattccg
     2821 gttaggatgc caatagaaat caagcctgtc cagagagacg aaccaccata agacacgaaa
     2881 tacaagggta ttccagttac tggcattatt cccattgcca ttccaatatt gatcataaaa
     2941 tgaaaggcaa atatgcctaa cagccctccg atgacgaatt gactaaagcg atccttgcat
     3001 gtttctatga gaaaaacaca tcgaaagaat agaattccat atagaataaa gagcacagta
     3061 caaccaataa atccaaattc ttcagctatt atagaaaaaa tgaaatctgt gctctgctgt
     3121 gggatatatc ttgcatgact atgagtgccc tgtaaaaacc ctttccctaa aaagcctcca
     3181 gatccaatag cagtgatgct ctgaagaatg ttccatcctg agccttttgg atcgatacta
     3241 ggatcgataa atacgagtaa cctcattatc tgatatggtt ttaagatgct atgagctgca
     3301 acggctccaa tcatcgaaat ggccaaaatg gccatactat atgagaacca gtaatagtat
     3361 cgcttcttga acttaagcca tccgattccg gaaatgatcg aaatcacaag cacaaagata
     3421 agtatagcaa tcagtacatt ttcatgagtg atcgcagtgt agatgatatt ttcagggttg
     3481 gggtgattag atgcatacaa tggaagactc agaaatagaa aaactccaaa tatcgataaa
     3541 actccaaata gaatatatcg aaaatcaaca tccgccaatg tgagcattac aagagcagca
     3601 ggaacaaata ctagtgcgga accgaaatca ggttgcgcca gaattaaggc caatgggata
     3661 atcataataa tgccggttat caagagcttt ttaaagcttg aatcaaattc cgcggattcc
     3721 aggtagcgtg cgagcatcaa aactgttgct acttttgtga attcggctgg ttgaatgccg
     3781 atgccgccaa ttccaagcca tgatctggct ccattgacca ctcttcctgc cagcagcgta
     3841 attataagca gtaatattgc aaaagcaaag aaaacccata caatatcctt atatctggaa
     3901 aaatcgaaaa gcgctgctgc aattagtaag aagattccga taccaaacca taagatttgc
     3961 tttaggtatt cgttagaaat aatggctcct tctgcatcga tccccgatga atatatggct
     4021 attattccca gtgcgcagag caaaaagcat gagaaaagta gataataatc aatatctcca
     4081 aacagtcttt taaaattctg gaatttcatt ctcttctccc tatcaaggaa gaaccctcca
     4141 gacgcacccc gacggctttc gccgcgtcat gggcattttg attagcatat attgcttgat
     4201 agattacatt agcagcataa ggagcccacc attcccaagg attcgaggct tctatcatgg
     4261 tggtcacgac tatctgatct ttagccggcg catcgtaggg tccaaaagat acaaaccatg
     4321 aatgccatct atccttgaga cccacttcag ctgttccagt ttttccggca acctgaacca
     4381 cttttgtgtt tattggcact ctagccgtgc catggacaat gacatcgcgc agataagatt
     4441 tcagagtttc aaatgtcttg gctgaaattg aagactgatg aaggatttct ggctgtattt
     4501 catcgataag agcgcttgta gtcggatctc taacttcttt tagaatatga ggcttataaa
     4561 tgattccatc attgacgatc atggccatca tatttgcgag ctgaagagga gacgctaaca
     4621 gatatccttg tcctatagag aggttcatgg tatcgccatg tgtccaggta tcgttatact
     4681 tttcttcttt ccatgtcggt gttggtaaaa gaccagcatt ctccgcaggc aaatcgatgc
     4741 cggttgcctt tccatagcca aaatcttgcg cataactgac aatattctct attcctagat
     4801 aatcccgacc agcaatccaa aaatagatat cacatgattg tgcaagggca ttcttaagat
     4861 ccagagctcc atgcccaggc ctcttaatat ggcagctcca tgaacgacca ccatatatca
     4921 gctcaccgct acaatagatt tttttatctg gagaaattga accttcttcg agaataccgg
     4981 ctgttaagac tgttttgaat gtcgaagcca caggatagga tgactgatag gctcgattga
     5041 gcataggttt ttttggatca ttcagaagat ctatgtatgc ctttccgata ttgtccgaca
     5101 tgaaaactct ggaatcataa taaggatagg tgaccattgc taaaatttca cctgtcgatg
     5161 gcttaagtac aattatggat ccctcgcgag agccaagagc ttcctctgca attctttgaa
     5221 tattgccgtc tatgctcaga atcaatcgct taccgggaat cggttcttgt atttctgcag
     5281 cattgctcga aatatctttt ccataaacat cgacggcttt agatatccag ccatccttac
     5341 ctttgagaag ttcttcatgc tctaattcta tgcctgcttt cccggtaaga tcttctaata
     5401 tatagccttt attatatagc agtttatatt catctcgagt gattgctcct acataaccaa
     5461 tgacattaga aagcgatctc agatcagcat aattcctaag gggtttagaa ctccaagaaa
     5521 cgcctggaaa ttcatcaatt ctggctgcaa tctgtgtcac ggcttccagg cttgcatttt
     5581 tggctaccac cactcttcca tatgaatata agctcaaatc aggaaacttg ctctctatat
     5641 cctctgtagg aatgcccaaa agcaggctga gtttcttaat aacatcggat tttttcgaat
     5701 caggtaattc ggaaggcgta atatataccg aataggcttc tctattacct gcaagaagat
     5761 ctcgtcctga tctatcatat atttcgcccc tgggggcagg aattcttgtt gattggatgg
     5821 agatacgctg tgcttcgctt acaaataatt cttttctcaa aatctgcata gagaatagat
     5881 ttattgaata gacagataga agcgatatca gtagagcctt caaaatgcgc atgcgaagtg
     5941 tgattttttt tggaagcata gccattatat ctgccctctt gcgatgataa tgctcacggc
     6001 atagctttct tgggattatg ctcgaacaac tgcctaataa aagaaagtag aaagaacagc
     6061 agtattgtaa ataatccgtt caacatcagc tcaataccga attccatact gaaaatatga
     6121 taataatgaa tgccagccga aaacagaaga tagattactt tagataataa cgccattata
     6181 atcgtcgctc ccgctccaag aaggaaagga acaataaaaa tgtccttctc tgtaacatag
     6241 cttataagcg ttgcaaagta gccacagctt gtatatagaa aggcataata tccgagcgga
     6301 gatgcgctga gaatatccgc cgcaatcccc accaagaatg aagcaatgat accctgccct
     6361 tctctgttga taaaagagca aaagagaagc acaccaagag ccagatcagg aataatgccc
     6421 atgataatcc cgcgcgacaa ccatgttgat tgtatgaaaa gcataagtgt cgcaacaatg
     6481 cctgatataa agatactttt cattgaattg cctctttttt aggcgaggaa agaggttcaa
     6541 tatctattgc gaacacatac tctatcctgc tgaggtcaat tgccggttgc acacttatca
     6601 ctaatgagct ggagagttca ttgagtcgcc aatcctccac tcgtcctata atgatctctg
     6661 atgggaagat ctcatccaag ccgctggtaa caacgatatc tcctcgtttg aggagatccg
     6721 cctcggcctt ggggacataa agcagattaa gtgggacatc ccgaaatccc tggccattta
     6781 gcattccttc ggcacgcgtt tttgcaagtc tggcagctgc gaagaatcgc tgatcataga
     6841 gaggttggag tatacttgtc gaggccttta cctcgattat cttaccaacc aaaccctcaa
     6901 caccattctg ataagccaca actggcatat atttccttat accatttata tatcccttat
     6961 ctatcaccag tgaggaatac atattggtag gatctctagc tatgacatga gcagcaattt
     7021 tctttgcatc tggcaatgtg ctaagcccta agagctcttt gaggcgttga ttttcttctc
     7081 tcagtgctgt gtaatctcgt acctttagct cataatcctg gactttctcc agtagtgctt
     7141 catagtctct ctcaatgttt cgaagctcag atactgcacc tatggttcca gtaaagaagg
     7201 agccaattgc agataagccc ttttgaattc ctccaattat ataaccattg aaaaaaccag
     7261 gcactttgac tattgtcttt gttgatataa atagaacaat aattgacagc accagacaaa
     7321 ttccagcttt cttgtgcatc gctcgagcag gtccatggat ttggctgcca gtcttcatgc
     7381 tcaacctgcc agtatcagag atttagactc tcgtaaacac tgctgcttcc gatattcttc
     7441 tgcataatct caaaatactt tccagcgcca atggcaacgc attcaagagg gtgctctgaa
     7501 agaattacgg gaacaccggt ttcctttgct atcaattttg gcagtccttt aagaagagct
     7561 cctcctcccg aaagcacaat accgcgttcc acaatgtcgg atgccagttc gggtggggtt
     7621 ttccctagcg ttttctttat ttcctcaact ataagattta ctgaatccat tagcgcttcg
     7681 cgtatttcta cggaatcgac ttcgaaacgg cgaggaagcc cagtgattgc atctgtgccc
     7741 tttatttcga ttttttctat tgttctttct ggatttgcat ttccaatttc tattttgagc
     7801 ctctcggcgg tctgctcacc tattatcagg ttatgtatat ttcgaatgtg ttttgcgata
     7861 gcttcgtcga attcatcgcc accaactcga attgcatttg tcactaccat gccttttaat
     7921 gatataaccg aaatttcggt agtgccccca ccgatgtcac atatcatgtg cccagcaggt
     7981 tctgttatgg gaatgcctgc tccaattgcc gcagcaagcg attctgctat taccttcacc
     8041 attctggcac cggccttata agtggagtct ataaccgcat tttcttcgac ctttgtaatg
     8101 catgttggaa cgccaactac tatccttggt ttgaaaaaaa ggttcttggg aaataccctc
     8161 tgaataaaat atttgatcat tttttcagtc atatctggat cggcaattac accatcccgc
     8221 agcggacgct ttgctataat atcagtcggg gttctcgaaa gcatgctctt ggcttcggta
     8281 ccaactgcta caatacggtt tgtccccctc tccaccgcca ctacagatgg ctcgtcgagc
     8341 acaatgcctt tgccattcat atagatcagt gtattgcatg tcccgagatc gatgccaata
     8401 tcagggctga acatgtcaag gatacccact tcctttctcc ttttcctata atttttcggc
     8461 caatcgcttt cttgcatccg catgattggg gtcgatttga acaacctttc tgaaagctgc
     8521 ccgagcacca agctggtcgt tcaactttgc atatatgagg ccgagaccat accatgcgtc
     8581 cacattgacc ggatcttctt ttagtactcc ctgatatcta tcctgtgcgg attgaatatc
     8641 gccggaattg aagttgtcct gagccagcaa gagcgtagct ttgagtctga tttttgcatc
     8701 ggatgccgag ctctccaggc gcaaaagcaa gtctcttgcc ttctcaatct ggtctgtatt
     8761 catgtaactt atggcagctg acaataaaag agcgtcagaa gtgttattct caagagctag
     8821 ttcaaaccag gaaacagctc gactatagtc ttttctgcca gcatatacga ttgctaagta
     8881 ctgtggaatg tctttctctt tgcttccgct ttcttttgca agcaagaaat aacgttcagc
     8941 aagatcgaac catggttcac ctttctgata ataagctttt gccagtatat aatagacttg
     9001 tcccttcatc cttgagggaa gtccaacggc gagggctttt ctgagagcaa taaccgcttc
     9061 ttccaactct tgttgtcgaa tttcctcttc ctgagcgttg agagcggaat aataatatgc
     9121 tataccgcaa atactaagat aatattcgtc gaaaggatag ctttctatag ctttctgtgc
     9181 ttcggacagt gtctgttcta tattatttcc ggaattccag actttagcca aagcggatcg
     9241 ggatatgcta ttcttagcac ttctgcctct gatggcaaaa tagaaaccaa ggaaaacacc
     9301 tacagcaatc aatgctatcg ctatgaaaag ccaagggttc ttcttttttg tgtgatgagc
     9361 ttttcctcta aagaatctat ccagcattaa aaataatata tcaaaaaaag gcgggcctgt
     9421 aagccgggtt ctgtatgtct gagcgatttt cgcgctctca acatggttac catctatctc
     9481 gatgcatcat tactgatgca ttccagcggt ctacccgcga ttttgtcgct tatggcgact
     9541 tggacgggtc atccatcgct gtttgacctt gctcgcgatg aggtttgcca tgccattccc
     9601 gttaccggaa atgcggtgag ctcttacctc accttttcac ccttgctgcg tattagcgca
     9661 gcggtatatt ctctgtggca ctttctgtac aggagagagc ttgcgttccg agcattcgga
     9721 actgaaaacc cttcctgccc cggatgttat ccggcatcga acccttccga gcccggactt
     9781 tcctcaggaa ttacctgcgg caacccagcc cgcctaaaac aaaatcattc ttccatatta
     9841 tcgataagaa aatcattgtc aataagcggc tcagtctgat tctttctttt tttggaagag
     9901 ctttttttag acgaatccga ggtgtcttcg gattcgtctt ccgactcagc atcatctgca
     9961 tcttcgctat cttcgtctcc caagtccaca agagaaccag tgagtatttc ttcgatcgat
    10021 ccttcaatct ccgtctcttc ataatatagg attcgcgaac aatatgggca aaaaactatc
    10081 ttatcagctt gacggacctc gtttacaaaa tttgcgggaa gaatcatgtg gcaaccagtg
    10141 catacaacac catggatcgg cacaatgccc atgcctgatt tacttcgaat gatgcgctcg
    10201 aacttgaaca tcaaatcatc ttcaagttcc tgagtcaggc tattctcctt ggttcgaaga
    10261 ttctcaatct cttcgataat ctctttacta ttctggtcta tctctgctcg tttttgctct
    10321 atttcggatt ccagagcgga aacagagatc tctatacgat gcaacctatc atcgatctcg
    10381 gcaaaacggc gttcttcttc ttggattcga tgcctaagat cctgttcttt ttctgaagct
    10441 tcacggattt ccttgtttac agcatcatat tctcgctgag tctggatgcc gtccatctgc
    10501 ctttcagcat tttcgcgcct catttctgct tcggcgagtg cctgacggag ctgcttaaga
    10561 tttgcttctg tcgtagtaaa ctgttctttt gcatccacca aggatttttt ttctctggaa
    10621 agcatttgat tctggatctc aatagtcttg ggcatggctt ccagctgttt ttctagttct
    10681 acacgattgc tgagcagctc ttggtactcc ctgagagtct cgaataaatc atccattctc
    10741 atgggcatgg gtcacctcta tcaatgatct atatagtcac gaagcttctg acttcgtttg
    10801 aattgtcgca atttcttgag agcttttgcc tcgatctgcc gaatacgctc acgagtaaca
    10861 ttaaaataca aaccaacctc ttccaatgtc aatgaatacc cctcatcgag tccaaaacgc
    10921 attctcaata cttcttgctc gcgttttggc agcgtggaga gcaccattcg cagttgctcc
    10981 tgtagcagat tgtaattggt ttgaacggct ggattttcta cttctttatc ttcgataaaa
    11041 tcttgcagaa gtgaatcttc gtcttctccg attggtgtat cgagcgaaac aggttcacga
    11101 gccacgtttt ttaccgcttt tactctctca atctcccagc caagttgcga tgcgatctcc
    11161 tcatctgtag gctcgcggcc gagcttctgc atgagctgtc tggattctct aacaaccttg
    11221 tttatctgct cgatcatatg aacagggact cttatggtcc tggcttgatc ggaaatcgat
    11281 ctagttattg cctgacgtat ccaccaggtt gcataggtag aaaacttata gcccttacga
    11341 tattcgaatt tctcaacggc ctttataaga ccgatattgc cctcctgaac cagatcaaag
    11401 aactgcatgc ctcgattagt gtattttttc gctatcgaga caacgagtct tagattcgcc
    11461 ttgattagcc tatcttttgc gctcttcatc atttttcggc cacgattcac ttctctagcc
    11521 atagatatta tatcgtcgat aggggtctcg aaattattct cgatatcctg cagttttttc
    11581 tcattgactt gaagctgtcg aattttatct ttgatctctt cagcgctaag gccaaggcgt
    11641 tgctcaattt cctctcgttt ttcttttata gttagatttc ttccaagggt tcttagatcc
    11701 ttggaagatg agacctgcaa aatcccctgt attctatctt gctcttttct atagcggatg
    11761 atctttcttg cagcaacgat gaatttctcc gaaaagattg ttatttcctc aggatgaata
    11821 tcagtctcgt tcacaattat aagcagaggt tccctgatcc ttattaaatg tggatcttca
    11881 aagatgttcc cgccttttgc gattatgttc tttttagact cgatatagaa cctaagatca
    11941 gcggcgcttg aatcgatgag ctctctataa aattggttta atcttcttcg ttctgcaatt
    12001 ttctctgtat tttccttttt ttgcagagac atgtcttttg gctctttgcg agtcgcttta
    12061 actactattt gatggaactc tgggataagc attccggatc ttcgtagtgt gttcttgatt
    12121 atattctcgc cttgctccat cattctcgag agctcgactt cctgctcggc agttaagaga
    12181 ttttctctcc cgatatctct cagataaagc ctaactgggt cgtcgcttga tatctccttt
    12241 tcacttgaga ccatcttgct cttcaaggga atcgaagtga gtttgggaag agcttcctct
    12301 tcaatctgga cttcttcttc tatttttaca tcgttgttta ctagaaggtt gatgatagct
    12361 tccattttat ccgaattgag tatttgttca ggcaacatat cagagagctc atcaaaactt
    12421 atggtctttt tttcttttgc atattcgagt agctttgcaa ttgcgggatc gagttccata
    12481 tcattcatca atatctcctc ccgtagtttt gagttctcca tcgaccttaa ttatttcttc
    12541 gaggatcagc ttttcttgta gcggatctgt atccgccgaa aatgatttga gacgagctac
    12601 aagctctctc cgctttcttt ctaaagcagc tttctttttc tgatccaatc cgtcctttat
    12661 gatcttctct agattgccct gtagttcacc ggaggtatcg acttctctga caaagcgcgc
    12721 ggcagcctca tcagtacaga gagacagaat cccttccaca tccaaaatcc cttctttatg
    12781 cgcttcctga agggctgaaa acagatcttt tgcccttgga tcgtcgagat tttctatatt
    12841 caaattctca tctacaaact ggaatggatc ttcggacaaa atgactgctg ccataaataa
    12901 aagatcggaa gttcgggcag aattcgagct gctatatggc acattagaaa gattgtagga
    12961 atccctaacg cggcgtactc cggatacttt cgctttagcg tagtccatag cgaccgcatg
    13021 agtgctgact ccaatgcttc ttcctatttc cttgatgtat tcttgtctct taacatcgga
    13081 atcgagagca tcaaggaagg gaaacagaaa cgaaaccgct ttcgcctttc cctccattgt
    13141 tgatatatcg aaaagctggt tggcacgtgc aagcagaaat tgtccagcgt ttatagtatt
    13201 tttagccatt ctcgtcaact cccctgcccc cctcttttcc aatatctctg aagcatcttt
    13261 gccttcttcg agaagaagca ctcgagtctc taagccggca gccgtcgcga tcgagcaaga
    13321 tctctcggca gcacgcaacc cggcctcgtc cgtatcaaaa cagagtatta tattcgatga
    13381 tcttcggcgg ataaccacag cttgctgact agtgaatgcc gtccccagag gggcaactgc
    13441 atttgtcagt ccagccgcgt gaaatgaaag cgcatccatg tatccttcgc atacaatggc
    13501 gttatctgta ttcttgatag aatcaatcgc ttgcgagaag ccaaatagtg tctcttgttt
    13561 cctaaagatt gccgtctcgg agctgtttat gtactttgga ccatcttccg tcatggctct
    13621 gccaccaaag gctattattc ttcctttagc atctccaatg ggaaatatta ttcgatcggc
    13681 aaatagagga tatgacagat ccttctttga aaaaaggccg ctatgtttca gaaaatcatc
    13741 cgaatacccc tttgctttga gaaatgaata tagccattgt ctatttgcag gagcataacc
    13801 tatgctgaat ctttcgatta gttgatccga taaacctctt ctatgaagaa tctccaaagc
    13861 tatgcgacca caatcatggt tcgtcaaaaa ccaatgaaaa ctcttggata gcctttcgtt
    13921 tagctcgaga agagtttgac gttccttttc agtttcccct agtttttcgg actcttcgaa
    13981 ttgaatctgc acaccgcatc gttgagccag ttcttgcatt gcctctgtat agctcaactt
    14041 atctatatcc ataataaatt gaataattga accaccctta tgacacccaa aacaataaaa
    14101 gaggcctgtg tcagggctta cagaaaagga cggcgtcttt tccgaatgaa aagggcaaag
    14161 tccccaaaaa gaagctcctt ttttctgaag gcggacatgc tcctgatata ctgataagaa
    14221 atctgtctta tcgagaattt cctgcctaat ctgtggtgat attctcgcca ttcttttcta
    14281 tagccccttg ggaattatct ccgaatagtt gtgttcttcc aaagtatgag agaagcgatg
    14341 ttttccagta ttaatatcct ccaatctaaa atacaaataa tctgtcctag ggggaaatag
    14401 tgcagctttt aagctgattt ctcccggatt ggagattggt ccaggaggaa gccctcggtt
    14461 gatataagta ttgtatggat cctggatctt taggtctcca taatgcacta ccgaaggcgc
    14521 ttcttttccc agatgttcgg tcattacata tactacggtt gcacaagact gaagaggcat
    14581 attcttccga agccggttaa gaaatacaga agcaattaat ggtgcatctt cggccaatcg
    14641 atattctctt tcaataatag aggcaagaat taccctttga tgaagagact gaggattcag
    14701 aatctctgtt ttggaccctt cgattctttc tattgcggca aaaaaacctc tgaccatctg
    14761 tgaaacgagc tctcgtgagt cgctttgtgg atcaaagaaa tatgtatccg gaaaaagata
    14821 accttcacaa tttgaagcgg gaatgcctaa tgatctagca agctctatat cattgaccgc
    14881 ttccaaaaaa gcttctgccc ttgtgacatt gttcgattct aaaatcaagg caatctggcg
    14941 cgcagtcctg ccttccggaa tgactacttt ttgagttatg taatttcctt ttatcaattt
    15001 atttgctgca acaataggca attctaaagg cataaatcga taggtcccag atttgatgtt
    15061 gcgctcagaa cctctggcac gcacatatag ctcgaatacc cgggaggaag gtatgacatg
    15121 attattgtgg agctctttgg cgatgtctct gaggctggat ccacttttta tcttcacttc
    15181 gacactttcg cttctgaaag gaggagtcaa aatcaaaatc actatagcca gcaacaatat
    15241 caagaacaga atcaagagat tctgcataat ggatctgatg tgatttgctt tcattatata
    15301 tcccaattat tccttaaagc tgctagagcg gcgatttcat cgattgacag aggagcattc
    15361 tgtcgccttt caaaaaaagc atctaatatc ctatctgatg atatcagaga gccccttccg
    15421 ataccggggc attgtttgcc ctcttctatc cagctttgtt cattttctaa gattgtttcc
    15481 caaaatggat atgtcctgca ctgaactggc ctaaccggat aaacgctaca tccatcattt
    15541 tctagaaaaa tacagtcgtt gttttgtttt tcctttaggc ttaatgtagt cactatgcct
    15601 agatcgacaa tcctgcagaa agtcagtgca aatctctgta tcgaaagatc aaaatattcg
    15661 cttaaagcca taagatctct cttagaaagc cagacaaaac caggttggcc gctacagcaa
    15721 gcattgcagc gcatgcattg gaaatagatg tcttttattg gcaattcagg attcatatta
    15781 ccaccatcct gcaagtggtc tgaagagata atcaaaaaag gcctttgcgc acaacaaaca
    15841 aaaaggggct gcccatctct caggacaacc ccttgtgggg agagaaggat ttgaaccttc
    15901 gaaggctaca gccaacagat ttacagtctg ccccctttga ccactcggga atctccccat
    15961 tgatggacta tatgtccctc gagccatctc ccggaatcga accggggacc tcgagattac
    16021 aagtcacgcg ctctcccaac tgagctaaga tggcgtgcac aacgaacggc actatatacg
    16081 aaagatgatg gtttggtcaa ggcctttgag gattccgaga ataaagagga acagctgagt
    16141 atagctctca gaatacgaat tgcccattaa gagatggctc gtggaatgcc ctgggatgaa
    16201 cttcaactaa gcctacggca atatagccct gagaaattgc atgatggtct gagagagcat
    16261 cgggcattcg ttcttcggcg ccttcggtga gaaaacaata agtatatcca tccgatccgt
    16321 caaagcactt gaggttgtca atgtacttat tttttcctgg aacagtccat actgccttta
    16381 gatctctcga ttttgtagaa ggaccattta tattgatgaa ggaatcaggc ttccataagg
    16441 ccacaagctc tttcagatta gctgataaaa aagatgttag accttcatat tccaaattat
    16501 cacaatgagc tgcacatgat atggcaattg ccggaattcc ttgcaatgcg gcttgtcgtg
    16561 ctgcaccaca ggttccagaa tatacaatat ccgtgccaag atttggtcct ctattgatac
    16621 cagaaatgac tagatccggt ttttcatgga gaatagcaag tcccgcaatt attatgcaat
    16681 ccaccggtgt gccagagcag ctatagcgcg agtcaccaag cctacgcagc ttagccggct
    16741 ttttcaaagt catggcatga ctcacgccgg atcgctcatg ttcaggcgca accacccata
    16801 cttggtgcaa gctttttcca ttgaaatctt tcattgcggc aattgtatcg attaaagcaa
    16861 aaaggccatc cgattgaata ccatcatcat ttgtcagcag aattctcatg taatctccat
    16921 ttctagaata ggattggtat attagaatca atgctccaga attcgaaggc ctgcacctgt
    16981 tgagacttcg tggataatag gtttaaagcc gaatattctc tcataatctt ccagcttgtt
    17041 gcgataggct tgcttttttt cattattgag gattgtaagc gtgcttccgc caaacccttg
    17101 accaacgagt ctagaacaca gaacatcgtc tagctcaagc gctctctttg taagccagtc
    17161 tatctctgga caggaaattt cataaaggct ccttagagaa gcatgggatt tgtttacgat
    17221 tcgaccaaag cctatataat cctgacgcga gagactctct tccgcttcaa tgactcttct
    17281 tacttcttct agcacatgta gacatcgtcg gcgtttgcgt tcaggcacta ttcctagcaa
    17341 ttcgtcgagt tcttgaggcg ctatatcgcg aatggaacga ttatgatagg ggacaagaac
    17401 cgaaaggcat gctttgcaat ctgcgattcg ctgttctagt tctgcgtcca ccgaagagcg
    17461 aggcactttg gaatcagtga gaagcatttc ccagcgttca ggtaagaatt gcactcctct
    17521 gcgcttttta tcatgcaagt ctatgatcga aaaacttctt cctgcaggcg cagtaatccc
    17581 aagataattt gttagaggta attctttctc aaagaataaa ctctgcgctt tccaagcatg
    17641 aaaggctaat tcttcaagct taaggtctaa atcgtatagg gtttttaagt caagcgcagt
    17701 tgccatattt atcgcactgc tgatacccaa accaagcccc agtggcacat tgctctggat
    17761 ggttacattg aggccacgag gatcaagatt aaatacgcgc caaaaataat cgcacacact
    17821 ctttacatga ttagcccagc gatcttctct tttgtactta aatgtggcaa catttgcgcg
    17881 cttacgttca ttgaaatcag ctgcataaaa acggagagaa ttgtcttttc taaaagatat
    17941 cgcgactcga acttcaaatg aaagagaagc tgaaagcaca agaccatcgg caaaatcagt
    18001 gtgctctcct aatattttta ctatagttgg cgcagctgat gtacagtctg gagctgtatc
    18061 atactccgcg tggtgcatta taaaaatatc tttcattgca gacattgtat cctattcgtg
    18121 ctgcattttc aaccgaattt tctcacttcg tctagaagat gccttgtcgc ctcaagcttc
    18181 tttcttattt cctttgcctc ttccatgtat tttcgccttt ctgaaatctt tgcttggatc
    18241 gatctgcatt gccaggaatc tgctctacca agataccggc tgcatacttt attgccttcc
    18301 ctccattgta aatatgcata tacttggtta tatctgcgtt tcaggacaat actacccttg
    18361 ggtctcgatg cgcattcgct ctcgactctc tgaaggcgat ttctaagcaa tttttcttca
    18421 taaagaagag cttgagtaac tgtttccact tagctaaata ataatatgac gacagtctat
    18481 gcgcaatcaa tttctcgcca aaaaatcata actcacattt cttgcatttt cgagatttgg
    18541 caatatactt atacattaga gcactaaaat gagcgaaaaa aaaatcgcac gaagcccttt
    18601 gctttcggga attttctatc catctgaacc gcagatgcta atgtctgaaa tagacggtct
    18661 tagtctatat gcaaagcgaa taccatgccc tgcatttggc attattgctc cccatggaag
    18721 ctttctctac agcgggaaga tcagcatgga atcgtggggt gcaattctgg gttcgaatcc
    18781 ttcaattatc gtcattatgg ggccttctca tctgccctat gaagatggtg tttttcttcc
    18841 tgaatccaca agattcgata ttccgggtgc ctgcttgaat atagatttga acctcaggga
    18901 ttatctcctt aacaagatac ccatgttgaa aaaagatgat ctgccacatt tggaagaaca
    18961 ctcgatagag cttcaattgc catttgcatc tcattttttc ccaggggtgc ccatattgcc
    19021 ttgcatcata tcgggtaaga atcctctgac cattgagacc gccacgagat tgtttgaaga
    19081 aataagcaga tatatcggaa actatgctgt ctttgtacta tcttcaaatc ttgcggtaag
    19141 cgattctccg gaagaatgtg atttgctttc taaggatttc atctattctc tttccagacc
    19201 agttgccgaa tttactggaa acgtaagagc ttgttataat tccttttgtg gagaggcagt
    19261 tattcgagcc ttcaggattg catttccaga ggcaagagca gaattgctag attatgataa
    19321 ttcttcgaaa cacagagaat actgtgatga gctagtggta ggctatggag caataggttt
    19381 taagaggtaa attatggaat ttgcaatatc tccagaggat caaaagaaac tattgaatta
    19441 tgcgcgcctc acaatacatg cttcgttgaa aggcgaaaag gaaatcgatc ctccgcacct
    19501 ttctacatct ctaaaatgtg gagcctttgt cacattgaaa aaaaatggat cgcttagggg
    19561 ttgtattggt agaatccagg gcgacgctaa tttgcctagc actataaaac agatggcgct
    19621 tgcggctgca ttcgaagacc cacgttttcc gcccttgcac gaggaggagc ttccattgat
    19681 cgagatagaa atcaccctcc tctctccctt atacccgatc tctccagagg aaattgtgat
    19741 agggaaacat ggtcttctca tctatgctaa tggaagatct ggaatactgc ttccacaagt
    19801 gccaatagaa tttggatggg ataaagaaac tttcttagag cagctctgct ggaaggcggg
    19861 tctcccttcc aatatatgga aatctcctct tgcgcgactt tatggattcg agggtttcgt
    19921 gttttctgaa tcggagctta aagaagctag ctcttcggat acctgccctt gatgtacatt
    19981 ccgaattgtt gactcttttg tggccttacc agatagtcca actgtctttt tattcagaga
    20041 ttttttgatc cttttgtgcc agaagcttcg catcttttct gctgttctaa aagcaaaata
    20101 gagaaaaggc cttaaactga tatcataggc tccataacga tgtattattt ctcccccaaa
    20161 gcccgtctta aagagataga gtccggccat aggatgagac gcatctttat catcgggcgg
    20221 tataccaaag aaatcatatt tatctgcccc gaatctcttt gcatcgagaa tagcttgcca
    20281 ttgcaatgca taagtaggca ttagatttcg tttctcgctc gatgatgccc catagagata
    20341 ggtcgagata tttccaaaat gcaagacaat tatgcacgca agaacctcat ctttatgccg
    20401 agcaacatat agagacaaac ggggcctggt ggtagttata gaattttctt gagcaacttc
    20461 tgagatctca gagcttgttt cgaatagagt cttgtagtag ctctccggat gaatagctat
    20521 tctatctcgc ctagctgttt ctttataaag agacataaat aatggaagac tggagaaacc
    20581 ttcgtctgtt acagaaatgc ctttcttttg agatagtctt atattatagc gccatttagg
    20641 cttcatttta gctaatattc tttgttcttc atctcgcagg tctaagatga cggtgtctgg
    20701 cacctgaacg tcggttcctt tatacaagcc cgaaatatgg aatcccaaag aattttctat
    20761 atcaggcttt ttttcttcct tagtctttgc tggatctaca tcgcactccc atggcaaatc
    20821 gaaacgtatg aaaacatctc tgccaccaaa agattcgccc aaagctcgcc caagtgcagc
    20881 cagagtaatc caacgctctg gaatagaact caattccgct gctccaaaag gaacataaag
    20941 aaaagaaaaa agttttgcaa atttcctctt caaaacaagg atcctaccac tcaaatcctt
    21001 attatagaaa gagtatgtgc atgaataggc ttgccagccg gtgcgagatt taaaaagtcc
    21061 ccagaacttg gattgcaaaa agccctgatc acagcattca ggcaaacaat cgattgccga
    21121 tagaatgggg cttatttcta ttcttggtaa aacatccatg gcactctgct ctaatacatc
    21181 ggacattggt tttttactta gccaattttc ccatccataa ctttttcgag ccttataggt
    21241 tcgccatgag ccataagacg ctggcctttt atccaagcat atccatcttt tgcatacatt
    21301 ggtattctta aaaatgtttc gatatcgatg ctctcctttg gtaccgtctc tgccggattt
    21361 tccatgtcta tatcttctgg caatactctt tcaccaggat ttgcctttgg agcggtcaga
    21421 acttcgacga tatcgtttcc ctctggggtt ttggtcgatg ccgcgagaag catgccttta
    21481 ctctcaatcc cacgcaattt tgctggcttt aagttgttaa caattataat attcttgcca
    21541 agaagctcat cttctctata aaagggcaca agtcctgaaa caataactct ttctaagcca
    21601 ctgccatcgt ccagggtctc tatatatagt ctgtcggctt tgggatgtcg ctcgatcttt
    21661 ataatacgag cgacgcatag atctattatc tgatctaatc gatcattgtg atcttcttct
    21721 tcagaaacag ggagctgccc ctcgcttttg cgattctgct ctgccatttc ttctctttct
    21781 ttttgagagc cagaatattg agcacgaagg gaggcaatca gctcatcctc aagtctatta
    21841 aaaagaacct catgtttttc tattctttta aggtctcctc ttagaccaag atcagcccag
    21901 ccaagcttcc catgtcctat ttctatccct aaaaaagatg caagtttctc ggctgtcctt
    21961 ggcatatagg gataggtcat tattgctaga tctttcagca catagcatag gtcgcgcata
    22021 agggagcttg ctttttgggg attgtctatt ctagctttcc atggctcttc atactggaaa
    22081 cgcttgttgg ccaaatctgc tatggaaaaa atggttctga atgcatctcg aagatcagcc
    22141 agttccagaa gctttgaagt ctcttgttcg cgatccgtaa cctccttcca gaaggcttca
    22201 tctggagttc catcggggat aattcctttg taatagcgcg aaataaaagt tagtgtccta
    22261 tttacaaggt ttcccaaatt gcctattagt tctccgttta ccttttcctg gaaatcggcc
    22321 catgtgaatg tatgatctga agtctcaggt ctattccaaa atacataaaa gcgccaaata
    22381 tcagcaggaa tcccagtatt catagcgtcg tttccaaaaa cgccaatacc cttggatttg
    22441 gaaaacttgc cagcttcata attgagatat tcggtactgg acatatgatg cagcatagtc
    22501 catttttttc cggtgcccag aaggctggaa gggaaaatga cagtatggaa cggaatatta
    22561 tctttaccta tgaactggaa aagttctact tcgtcgggtt tctgccacca gcttttccag
    22621 tcgaaccccc tttcctttgc caatcttgct gtcattgaaa tataaccgat aggcgcatca
    22681 aaccatacat agaagacctt gtccttaaat ccctctttgg gtacgggaat cccccacttt
    22741 agatccctgg tgatagcgcg tggatgcaac ccatctcgta tccaagcctg tgtcatctgt
    22801 attgcattgt ttgcccaaaa gccttttaca cttgcgtctt tcatccaggc ttccagctta
    22861 ggaagtattg caggcagatc caaatataga tgtctggttg aacgtggtct tggagtacct
    22921 ccgcaactta tgcatttggg attcaaaagc tccattggat cgagcaattt cccgcacatc
    22981 tcacattggt cgccacgtgc tccatcataa ccacaatgtg gacaagttcc tcttacatat
    23041 cgatcggcaa gaaaacggcc acaagtatcg caatagagtt gatctatgga ttgacttgaa
    23101 atatagccat tgttgtctag atctagaaaa atggattgcg tgatctctgt gtgctctgga
    23161 gtcgatgttc taccgaaagt atcaaaagat atttcaaacc attcatagat atcgcggtga
    23221 attgcataaa acctatcaca gagctcacgc ggactcactt tttcctcgag agcttttgtc
    23281 tcagttgcgg tgccatattc atcggtacca cagacataca aagtctcata tccactaagt
    23341 ctacagaatc tagcaaacac atctgcagat aagacctgaa tcagattgcc aagatgagga
    23401 acattgttta catatggaag ggctgaggta atgagtcttc ttttcatggc ttggacaata
    23461 tccccgtaga aaggggctgt caaggacagg ctcaagtgat aatattatat agaatggttg
    23521 tttcaatgtt gcgattggtg ctcaaattga atgagacatc cactatcaaa gataaaagaa
    23581 gagtagtttc cggaataaag gacagacttc agcacagata ccgactttct tgcgccgaaa
    23641 ctgacttgct tgattcactt gtttttgcag aaataggagc tgcgctagta tctaattccg
    23701 cacagtacgg agaaaaagta ctgcaagatg ctgtaacgtt catagaggac cattatccgg
    23761 tagaactctt tgaagtacag atgcattacg agatatacgg atgagagggc taatccaatg
    23821 aaaagtctcg ggaaaatatt gctctttatg gcttttacta tctctctgac tttatgtttc
    23881 atctcttgta tcgggattga agcttcaacc aaaatcgatt ccaaaggtgc gggaacattc
    23941 tctgttgtat atcaaatatc taaggacttt gtggatttcg gatcagaaga aaccacccct
    24001 tccctacccc ttgccctctc tcgcgaagat atcgagcgag gattacaggg ctcagaaggt
    24061 atttccctga aatcgtactc aaaaacaaat aacgccaaca atacaattat ttcttttgcg
    24121 ttgaatttcg cttctccaga gaatcttata aactacctcg atcctacccg aagttatact
    24181 agattcagcg aagaaaatgg cagctattct ttgaaattcg attttggtaa tgacattccg
    24241 cctcttgatc cagatatgaa gaagtacatt tttgaacagt tttcatctta tcgatttatc
    24301 ctgaccattg agaccgcaaa atctccccct cctgaggtct ctattaaata tggggatttt
    24361 attcgtaaaa caagctctgg caaaaagacc atcctggaat gcgcaatggc tgatctttta
    24421 actgtgagct cttcccccca aatcgaaatc gtttggagat gacgatagat ataacgatat
    24481 aaagcaatgt cttaattcct ttaagcagaa taataagata attaacaacg ttatttatac
    24541 atagcttgac taattttgcc gtttcgatta gttttccacc gcagcgaatt acttattcgt
    24601 cagcccgttt tctatatatc ggaggaaaaa cctcatgcca agaagaaaat tcgtgtatgc
    24661 atttggtgca ggccttgccg aaggagatgg ctcaatgaaa gagctgctcg gtggcaaggg
    24721 agcaggcctt cacgagatga ccaaaattgg acttccagtt cccgccggct tcactatcac
    24781 cacagaagta tgtgagcttt attacaaaaa tgggaaaaaa tggccggaag ggcttgaagc
    24841 agagatccaa aagcatataa agaatctaga aaaggtgaca aaaaagaccc ttggcgatcc
    24901 aagggatccg cttctcgtct ctgttcgatc cggggcacct gtttcgatgc ccggtatgat
    24961 ggagactatc ttgaatctcg gtcttaccga tgcttcggtc gagggtcttg cttctaagac
    25021 cggaaatagg cgctttgctc tggatgcata taggcgcttt attatgatgt atagctctat
    25081 agccatggga atagataggg aaatattcga taaagccttt gaggaaatca aagagacaaa
    25141 gacaaggcca cgcataggaa taccctccga acaaaaagta aacgataccg atgtgaacga
    25201 agaagaactc ggtgagctta tcaaatcctt caagaagata tatcgcgatc atattgggac
    25261 agattttccg caagactcat gggaacagct aaaaggcgct atcaatgcgg tcttcgattc
    25321 ttggatggca gaaaaagccg tgacataccg aaaagtagag aagctggtgg gaattaaagg
    25381 gaccgccgta aatatcgttc agatggtttt tggtaacaaa ggtgacagtt cgggcaccgg
    25441 cgtatgtttt accagggacc caaatactgg cgaaaatatc ttctacggtg attatctcat
    25501 taatgctcag ggcgaggacg ttgttgcggg aattaggacg cctataaagc ttgcagagct
    25561 cgaaaagctt gacccaaaag tatacaaaca gcttgtcgat atccgcagaa agcttgaaag
    25621 acactataga gatatgcaag atctggaatt caccgtggaa gaaggtaagc tctatatgct
    25681 gcagtgccgt acgggaaaac gtagccctat tgcagccttc cgcatcgcag tagatcttgt
    25741 aaaagaaaag ctgattacta aagaagaagc gatacttcgt atcaaagcct ctgatataga
    25801 gggaattttc tatccaatga tcgataaatc gaatactaca gcgcttaagc gatcttttct
    25861 tgtgcaaggt atcgatgctg ttccaggagc cgcagtaggc agggtggtat ttaatgcaaa
    25921 ggtcgctgag gaatgggaag aaaagggcga aaaaatcatc cttgttcgca aggaaaccag
    25981 tcccgaagat gtaggcggca tgcatgcagc tcaaggtatt ctgacagcta ccggtggcaa
    26041 gacaagccat gccgccgttg ttgcgcgagg atggggtaaa tgctgtattg tgggctgcga
    26101 aaagctcgaa atagactatg aaaaccgcca ttttgccgtt ggagataaga tcgtaaaaga
    26161 aggagattat atcactctgg atggcaatac cggaaatgta tatcttgggc agctcaaact
    26221 tgttaagccc gagcctccta aagcgtataa aactcttatg tcctgggtag acaggaccag
    26281 aaagctcaaa gttcgcacca atgccgacac tccttatgat gctgaaaagg ccagggagtt
    26341 gggtgctgaa ggaataggac tatgccgaac tgagcatatg tttttcgatt cagaagagcg
    26401 tattcttgca attcgcgaga tgatcatagc ggatgatacc gaaagccgta aaaaagccct
    26461 ggctaaactc ctcccatttc agaccaaaga tttcgagggc atatttagag tgatggatgg
    26521 tctacctgta actatccgcc taatcgaccc acctcttcat gaatttgttc cacacgatga
    26581 agaaggtcag cgcgagcttg ccgatgcaat tggtgttcct attgaaaagg taattcagcg
    26641 agtagagcaa ctgcatgaac taaatccaat gctaggtcat agaggctgcc gtttgaccat
    26701 aacctatcca gaaatcctgg atatgcaggt tacggcaata atcaccgccg catgcaatat
    26761 gaccaagaat ggtataaaag ttcttccaga gattatgatc cctttggtta tagatgacaa
    26821 agagctaaaa attcttgagt caagagcacg tgaaatagcg gctgccataa tagcggcatc
    26881 agaagtgcca ttgaaatata tgatcggtac gatgatagag gttccgcgag ccgctttgct
    26941 cgcagataga atagcaaaag tcgctgaatt tttctccttt ggtacaaatg atctgactca
    27001 gatgaccctc ggtttgtccc gcgatgatgc cggtaggttt ctgccagact atatcgatga
    27061 aaagaaggga gcgattttca aagcagaccc atttcagaat atcgaccagg atggcgtagg
    27121 tatgcttata cgaatgggaa tagaaaaagg ccgctcatcg aggcctagac tcaaggtagg
    27181 tatctgcggt gaacatggtg gagatccaga gtcggtaaaa ttctgtcatc gagtaggcat
    27241 gaactatgta tcctcttctc ctttcagggt gcccgtctct agactcgctg ctgcacaggc
    27301 ggtgattgaa gatgctcagg ccgcccataa atctacaaga aaacctggta gacctccaaa
    27361 ggccgcatcg tccaaggcaa aacgccctcg gggcagacca cgcggttcta aaaataaata
    27421 accaaatctt gtaatatttt ccatgttcaa aataaagaat aggagcgata ttttagcgct
    27481 cctattcttt tgtttccaac ctcaataaat gtcaaaaggc ttttttcgaa tcaagcaaaa
    27541 ttgtgacagg cccatcatta attgaatgaa tccgcatgat ctcttggaat ttgccagttt
    27601 ggatatggac tccgttctcc agcaattttg tgcagaataa atcgaatagc atcaaggctt
    27661 tttcggaacc agcggcctta gaataggaag gccgtctgcc ttttcggaca tccgcatgca
    27721 aggtgaactg ggaaattaca agggcttcat agccaagatc gagaatcgaa agattcattt
    27781 tatcattact atccataaaa atgcggaggt gagctatttt atcagcgaga tattcagcat
    27841 cgtcatctga gtcatccgat gcaattccga gatagattag tatcccttta gtaatgcttc
    27901 cacagaattt ctcttctctg gatgttctat atgtcacaga agcctcggat actcgctgta
    27961 ttacggctct catctaaagc accaccctta gttcgattga ggggcggcaa gcccttcgct
    28021 ttgttcaagt aaattgagtt ttgtcataat atcctgcagt tcattgtcgg tgaggttttc
    28081 gatattatct atagccctgt ctatttcaga aataagatct tgaatggctt ttatatcgag
    28141 tattggcgca ttgactatag attgctcctc caatacgaca ggaggactct ccaagacttg
    28201 ttggggttcc tggcggggga aaggataatt tagcgatagc caatcgatga attcgctacc
    28261 tgatccaccg gtgagccaag tgcttgccac ttttatgctt ccctttggtg ggataattct
    28321 ctcattccaa taaaggccta gagcggaatc attgatagag aaagaagaga gagtgaagcc
    28381 tttcatgagt tgagaatcat aatagaactt tgaatcgctc agtcgcttcc agtttgcaat
    28441 tacaaccata tctggctttt gatcttcatc gaggagtagc gcaagggaag cagcctgtcc
    28501 tggagaaaca acgtattttt catcggaatc tgatgcaaga atcatttcgt cggtaaccct
    28561 ggcatttaaa cttgtagcaa aatgaaagcc ttctttctcg ccgagaaccg tatcgcatac
    28621 ttcttttatc atcactttca tttctcgcgt tgtatagttt tcgatttcta tttcgatgac
    28681 aaaaccatct gctacccgag aaaaggggct tgtaaaaaaa agcaatctct gagtcaatct
    28741 atttgtaacc gatctatatt cgatttcaat gccattcttt atttgtcgct ggctagtctt
    28801 atactccttt gaagctccca tcctatatat gcggttgtcg atattcagta caataaaact
    28861 cgtccttgga tccccactaa accacaatgg ttcatatttc gattttccag atatatttac
    28921 aaggcgataa agcgctggct gcagtgtcag atcatcagta gcaattctaa caagcccgtt
    28981 gcgtatatca atagcaaata gagcgcctat aactatgaac aataagatga aaaagcatac
    29041 tggttttttc atggtgttat atccttaaga tagttgtcta ttttccatat gagcaggttc
    29101 aaagcccttt ctttggcttc aagaagcata ttgagcctat tattccttga agtctcatcc
    29161 gcgctcaatt ctcgagtttc tatatttggc cttgcagaat actttctgat tgcttttgac
    29221 cataggtaat ctcttagatg tcgttgttct ccatatacat cttcctgcat ctgctgcaca
    29281 tctctataga tatttcttcg agactcaaat tcagcaaggc gagcatattt ggcttctcta
    29341 gattctgaag ccatcacggt aatcctccag gcggtgagag aagtggcttc cgtgttgggc
    29401 catgcctctt tgattttatt aaaaatggat agagcttgag cgaaatcccc aagctggtaa
    29461 tagctctctc ctttccagaa caacacggaa gaacaaaacg gactatcagg atatctatta
    29521 atgaaaatgt cgaaaaccga tatagcatcc gcatagagtc ctagcttaaa caaaattctg
    29581 ccttgcagga attgcatatc aggcatattg ggatgagagg ggaatgattg catgaatttt
    29641 tcacaaaatt cataggaagt gggaaagtca gctgcctgaa aataagataa tgcagccatg
    29701 aagcagctgt cagccaagat ttccatattt gactcggtct ggcatatatc tgcaaaagcg
    29761 gctcctgcac gcccaaaatc tccatttctg aaaatatcga atgcatagga gaattcatct
    29821 gatgttttgt tagcttgagc ataagagaaa gatgcaagaa taaacataat aaccgcaaac
    29881 attttcatta tcaacttttt catacttaga tcttcccttt ataatattta taatatatga
    29941 atcttggaaa gcccatatcc aggtcaatcg atatttccga atccaggtca atcgatattt
    30001 ccgattgggc ttgtccaggc attcctaaaa tcggcaacaa actgctttct atcctgagca
    30061 ttaaaaaatg cgttcccaat aacgaggaca tccggctgct gaatcacgat gtcggagatc
    30121 gatttcgcat ttactccacc atcgatggca atcaagaaat ggaggcccct ttgttctcta
    30181 attcttctga gttcttccac tttttgtagg caaaaaggta taagcttctg acccccataa
    30241 cctggattta cggtcataac aagcacaagg tctaagaatt caagaagcgg tactatatta
    30301 gagacagggg ttgaaggcac tatcgatatt ccagccttaa tgcccgcacg tgatatgctc
    30361 tggattagac ggtgtgagtg cacgcaggct tctaaatgaa aagtaattgc atcagcacca
    30421 ctctctatat aagcatttat gtggcgctcg ggttcttcta tcatgagatg agcatccaag
    30481 aacaattctg tcttcgctcg aatatccgcg atcatcttat gcccgaatgt gataggcggc
    30541 acaaaatgcc catccatgat atccaggtgt agccaaggac acccgctctc ttccacatgc
    30601 cttatagctg aaccaatgtc ataaaaatca gccgacagaa tcgagggcgc aattattggt
    30661 tctttcatat gctgtatatg ataacttgtt tcgtctagtt tttccagact ccttttttag
    30721 gcctaatgtc aatctaattg gaaatagcac atagaatagc cattgacgca aaattcaagg
    30781 ctcgggtact atttgaatag tagttaaagg agaagaacat tctaatggcc catcagcctc
    30841 gtccaaaaga agaagcaacc atagcagaag aaaatcttta tctggcttat gaaagcctaa
    30901 aaaatgcgga tattcaaagc gcagattctt ttctcgagaa tgctttgaaa gcggattttg
    30961 agcacccgga agttctcttt gcgatgaaat gccttcattt ctggaaggat ctcatctctc
    31021 aaatgccaga aacaggaagt cttttagagc gtggagattt tctctgtggt tcctggaaaa
    31081 agttcatatt attcctttct catataggag actcattcga tactgctcgc tacgtttaca
    31141 ggcgattcat atttggactt gctctggatc agtatcttgc cattccggac gaagaaaagg
    31201 aggcctttgg gccattgtta gatctgaggt tgggcagagc cagaaaagcc accggagata
    31261 tagaaactgc tcttattcac cttgagcggg cagcccaagc cagacggaat gatgcaaaca
    31321 ttcttgccga attggctgat gtatatgcta tgtcaggaga ggcgcgcctt tctaaggcct
    31381 tattcagaga agcctttttt attgatgcac aagccataga aattgatttc ttagagtccg
    31441 aattgatctt taagattatt gaaaatatac aaaaactagg gtattctgga agtgatattg
    31501 ctgaatggat accagtttat gcagaaatct ggggcgtatt caatattaag agagaattga
    31561 gcggtgctga atataaccga atttcagctt ctgcacgtca acttgagatt caattaagag
    31621 aatcgcatca gcaaagatcg agccttatac cccgccttct aaagaaatat ttttggatgg
    31681 cggattatct gaaggcccga gaggatacca aagaattgca ttcggtgctg cttaagatca
    31741 agattatcga tcaatccatc tatgaatcat atataggatg aagaggagaa gagaatggat
    31801 cagtctccat taagtgaaaa actacagctc atgttgaatg aagagaaatg gaccagaacc
    31861 ctaatggcga actactctat cgccaacctg aaagaactcg atcgctttat tgaggaaata
    31921 actgcccaga atgtggctga tgatatcatc aagctttgca tagagcactt atctcaaaca
    31981 aaaaatagcg ttattgcgct atatgtcgcc ggaatgctaa atataatgcg ccagccggcc
    32041 gatgaaactt atcttattca gttaatggat ttatttgcag aaagcaaaaa aagcaatatt
    32101 gtcgagtata tatgcaaccg gatgctagac tttggagaaa accgcattgc tctcgttagg
    32161 ttagcagaat gctatactgc agagaacaaa atcgagcagc gatacacggt atgggagagt
    32221 cttgtccgaa tcgacactga agaagccgaa ctagcacagc ttattgcaga atggaaggaa
    32281 aagaatggcc taatagaaga agccatcgat ttctataaga aggcactcat tcgctacatc
    32341 aataagggac tattttcaaa tataaaggaa ttatggcata agctgattca actttcgcca
    32401 gaggattatg acttttttct gcacgcgcag aagaaaatag caaaacaggt ttcgccggaa
    32461 aaagccgcca caattctaat ggacttatat gattggtata agaaaaacga aaagtgggat
    32521 accgcattga acatactgaa gcttattgtg gaatatgatg agcgcaatcc tgccttacgc
    32581 aaagaattcc ttgaatgtta ttcaaagata tatagcggac attcgatgtt cgatgaatgt
    32641 gtaaggcttt caaccctaac acaatcctat cgttcgctgc atgaaaccat ccctgacttt
    32701 gaaaaacgca tcgcttttga tagaggaaat tatgtcttcc atagaacctg gaatattgga
    32761 aggatttcaa atataaccaa cgatgaagtt gtcattgatt tcgcaaaatc gcggaatcat
    32821 cacatgtcgc ttaaaatggc ttttgatagt ctaattactc tttccaagga tcatatatgg
    32881 gttctaaaag caacctggcc tcgcgataag ctcagagaaa aagtaaagac agatccggcc
    32941 tggacaatca aaatcataat caagagcttc gataatcgcg cggatatgaa acgcatcaaa
    33001 gcggagctcg tgccatctat tctctccata tctgagtgga attcatggag cactcgcgcc
    33061 aaagaaatac tcaagacaga tc
//
LOCUS       DAOMZL010000009        32189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_1059_length_32189_cov_14.309423, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000009 DAOMZL010000000
VERSION     DAOMZL010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 32189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32189
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1059_length_32189_cov_14.309423"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1108
                     /locus_tag="PLQ30_01580"
     CDS             83..1108
                     /locus_tag="PLQ30_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPW01164.1"
                     /translation="MISVFSPYITRRDMDLVLSRMVEDAVSEDVFNRRFENAVKEQFN
                     LEYVISMRSPYNALQKALQVFGLERGAKIAISALAPAWQKDALEDSGFTPVILDIDQE
                     TFHPKPESISLANPSAIILFDALGKSPSEALLERIDIPIIEDISQTLGMIKVSDEKHL
                     YGHFLIWSLESDSAIATGGGALLCASSKRDAQILRLLDESMPRELKMTDYNAALGISQ
                     MKSYPKMIERRKTIYRRLVGQLLRTRHSYLQQEDSESCPLYAFPIFPETSVKEIIDYA
                     MKHGVESKHAFASCISQRLYESEPEQEAVFPIARSVALRCILFPMHHKLTNQQVEQIG
                     KVIATLP"
     gene            1118..1972
                     /locus_tag="PLQ30_01585"
     CDS             1118..1972
                     /locus_tag="PLQ30_01585"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPW01165.1"
                     /translation="MSESKSTLIFANLNKDDAGHAAQIVQAELEQNGWHTEILAFRGS
                     ISEYPDFDRYSLIVSLGGDGTLLYAARFAGPRGIPILPINLGTLGFIAANRMETWAVT
                     FEHWQKGALDPSRRLMLQSYAYRGKKILHKGIALNDIVVSSESGARMIRMCLSINREC
                     FGYYRADGLIVSTPTGSTAYNLAAGGPALHPEINALIINPICPFTLASRPLVIPASNC
                     IDIEIDETKKAGALLISDGHIVANLEKGDVIRIRKYEKSAFLIVPSGNAFFSALRTKL
                     GWSGDNDA"
     gene            1965..3662
                     /gene="recN"
                     /locus_tag="PLQ30_01590"
     CDS             1965..3662
                     /gene="recN"
                     /locus_tag="PLQ30_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPW01166.1"
                     /translation="MLEQLSVRNFAIIESIDIDLSAGMTVFSGETGTGKSLIVDALGF
                     LLGARVDNSIIREGATECSVSGLFSASSNAQVGLWLREKGIEWQINEGILLRRILKQN
                     GRTLAWIQDRQVSRNELLEFTQYLVDIHGQHEHQRLIDSSTHLDMLDAYANLEDELQS
                     YQVLYSEWREATRQLQEVLEQKAKRSQEADYLDFVIRDIASIKPKPNEDNELLTEEKV
                     LSEHEKLFGAVSEASVLLDSGEGGDVLHTLKRIRASIETARSVDIRLGSFSERLSAAY
                     YELEDISESLNKYKAGLRFDPDRLEKIERRLSDFQRLKHKYGPKLDDVLQTYDRAKRR
                     LEALEHAEENAEELEKKEKSLEKEVLNRAYQLSEKRMMAAVSMSSSIREIVKDLGMPE
                     AQVNLRLKRIVDERGGNRATLRGIDEAEFFIAPNPGEPELPLSRIASGGELSRFALAI
                     KAVLASHDEVETLVFDEIDSGIGGQVGVAVGEYLRQLSQYRQVLCVTHLATIAVRADH
                     QGKVDKFVSKGRTGASIRFLSGEDRETEIARMLSGFSESEASRIHAAELLRKAISHN"
     gene            complement(3729..4139)
                     /gene="cdd"
                     /locus_tag="PLQ30_01595"
     CDS             complement(3729..4139)
                     /gene="cdd"
                     /locus_tag="PLQ30_01595"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPW01167.1"
                     /translation="MTTEYTYLFKHALKACEQSYAPYSKFRVGAALLCDDDSIITGVN
                     VENRSYGLTICAERNAIAAAIASGKKRFKALVIATPDADYPVSPCGACRQVISEFMHS
                     SAPVIFGNSEDSYISSTVGELLPHDALHELACRG"
     gene            4243..5334
                     /locus_tag="PLQ30_01600"
     CDS             4243..5334
                     /locus_tag="PLQ30_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HPW01168.1"
                     /translation="MRRRFYGLIIGIAAIIFTLFSIRSTLPEREITSSVFLTFAEALD
                     VSRIPKARSDQIVPFIAESFLGYADPLEELLYVHPFKGRAAIDRLGWITYDRLATDLK
                     LYDLNGGGQQFQAYAYPWFKASWRVLLRADQMGIAVMDKTGLIRWEKEFPTPITAIDA
                     SSLFLAVGTLDGSISVFDPNGWAILSMQNSLQEFQTIYGLALSADSNYLIIVRGISPK
                     KIETYRFTDNSFAKVSESSLMVNTTFQATIAIARDNSHAIMSFGDMIIYYNIKNKYYR
                     ILEAGMAGVELSETQVDFRYFVLGSLKDSLVAILRSDLEGKAKSDVIILNHGLPVHIA
                     PGAFGAIGDEEVIGFVYKDGLELLKGWRP"
     gene            5331..6326
                     /locus_tag="PLQ30_01605"
     CDS             5331..6326
                     /locus_tag="PLQ30_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01169.1"
                     /translation="MKGKYPFLISYLWLATLLGALDFKIPEVIDLSGFGTVLGDQAKQ
                     GVSLEYDVRERCYGKIPRSDVQLISLDTTFEAPSSGAIFYSQVEADLPTVFSFPLGGA
                     LAVIHPDRFVSITSGLDPIQVIKSTSRSSKEVQNSFAIKKGDKLTGGDGSGVYPSKNF
                     GVRLFDAKNELWMNTIFLAPWIRDNTSPIMRSAKLIMTGTDKKVFFDIMETNKKEKLI
                     TCPQGNYSLFVDARDIIITSSRYYSAPYCIKVTLDGETVIDASFIAARASGEGISFLG
                     NPPPSRGSIGEGYSGEGYSYHVGQFLILRGKHDLMLQVNDFAGNQTTMRTSITAY"
     gene            6369..7103
                     /locus_tag="PLQ30_01610"
     CDS             6369..7103
                     /locus_tag="PLQ30_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPW01170.1"
                     /translation="MSNNRATIADNVTIIKERIWRAAEKAGRSSDKIDLMAVTKFQPI
                     DVIRQAYLAGIRIFGENRVQEASAKFPSLEPELEGASLHMIGTVQKNKINKALTIFDG
                     IQGIDDIETLEAILIRIKAPKRPIKLYLELHTGEISKSGFPNIDELLRGCEVYARYRQ
                     SDPQWMTKALLCGLMTMAPFTQDISLIRRSFKMLSAAKDKIKSSFDFPEFSELSMGMS
                     NDFEIAIEEGSTLVRIGTAIFGERNA"
     gene            7105..7590
                     /locus_tag="PLQ30_01615"
     CDS             7105..7590
                     /locus_tag="PLQ30_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01171.1"
                     /translation="MNRRCLLLLIVFLCGDLLVYCFPAQAQEENPGSEIVAKEEENSQ
                     EASEASVAPEAPAAPEISEASEAPKVTLPTFSYSDTKEGMFRRSLVISIGLFPFSYFY
                     TNFAFDLARYISHGFDTAYAPWPFNTQYGVALTNSEVWTRIGIASGASILLGFLSVII
                     E"
     gene            7678..8238
                     /locus_tag="PLQ30_01620"
     CDS             7678..8238
                     /locus_tag="PLQ30_01620"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HPW01172.1"
                     /translation="MANGSDFRGDYQEFNLDDAIRKIPDFPKPGILFYDITSILANPR
                     AFKYCIDSMFKLYGDTEFDVAAAIESRGFVFAAPFCYERNIPLLLVRKKGKLPGRTIS
                     CAYELEYGTAVLEMHIEDIKPKARFLIIDDLIATGGTINATCEMIKRGGAVPVRAFSV
                     IGLPSLKYQEKVTDVGIDTLIEYSGE"
     gene            8317..9987
                     /gene="gpmI"
                     /locus_tag="PLQ30_01625"
     CDS             8317..9987
                     /gene="gpmI"
                     /locus_tag="PLQ30_01625"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPW01173.1"
                     /translation="MLERLKKNPNWKNREGPLVLVIMDGVGYGTYKEGDAVLAARMDH
                     FRELEANCPKTRLKAHGTAVGLPSDDDMGNSEVGHNAIGCGRVFAQGARLVNISIESG
                     IIFEGKVWRELIANIKETGGTLHFIGLFSDGNVHSHIDHLKAMLVQAQREGIKRARIH
                     ILLDGRDVGSQSALEYVLPFETFLEEFRNQGSDYRIASGGGRQYITMDRYGANWQMVK
                     RGWDCHVRGIGRQFASAEEAISTYRKEIPGIIDQDIPEFVIAENGRPIGTIEDGDSVI
                     FFNFRGDRALEITAAFEQDNFDKFDRVRRPKVYYAGMMQYDGDLKVPSNFLVSPPAID
                     RTLGEYLAYTGLKTLAISETQKYGHVTYFFNGNRSGKFSEELEDYVEIPSDRVPFEQR
                     PWMKCAEIADYVINAIENGPYRFIRLNFPNGDMVGHTGVFQAVVCGLEGMDIQLGRIY
                     EAVRKKGGILLITADHGNSDDMYEHDKQTGRVILGPDGKPKAKTSHSLNSVPCVIYDP
                     QYQGEYKKELRQGMGISSLAATCAELLGYIPPDHYDPSVLDMPIENEA"
     gene            9995..10555
                     /gene="cyaB"
                     /locus_tag="PLQ30_01630"
     CDS             9995..10555
                     /gene="cyaB"
                     /locus_tag="PLQ30_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HPW01174.1"
                     /translation="MQNPEYSHNLEIELKARVQQREEVESRLSSFMHYIGPINKLDEY
                     WEVSNARLGDFGGFRFRVRHELASTTITFKEKTFDGDVEINHEFEFSVNGEPAFKAFI
                     EKMHAHLIYQKQKRGTRWEDGTGLVAEVVEVSSLGLFLEVECIGDSRVTLQQEEAKSR
                     LYRVIDQCGISRSALEPRPYSQLLGY"
     gene            complement(10826..11410)
                     /locus_tag="PLQ30_01635"
     CDS             complement(10826..11410)
                     /locus_tag="PLQ30_01635"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HPW01175.1"
                     /translation="MKKASVILISILVMTSCATVSDVTTLKRCEFRMQGIKDVVAAGV
                     NISGKKSISELSLLDAGRITLAIKKGSLPVTMTLAVEIRNPNTQTVAVDRVDYAVALD
                     GEPLFSGFTTDSVKVPGGNRVAVIPLKVTFDLFSLKEDNTQDAILNLLFNLAGASEIP
                     STLTLQLKPSISIGKAMIPYPGYMEISREFGGRQ"
     gene            complement(11526..11684)
                     /locus_tag="PLQ30_01640"
     CDS             complement(11526..11684)
                     /locus_tag="PLQ30_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01176.1"
                     /translation="MRIIYKMNGKGFVGSLVNLPAAYFRGRIIKETSSKIEREIRAYK
                     SWLENKAV"
     gene            complement(11950..12420)
                     /locus_tag="PLQ30_01645"
     CDS             complement(11950..12420)
                     /locus_tag="PLQ30_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPW01177.1"
                     /translation="MSLERAKEHLKKWNKDEQVRELDESSATVELAAKALGIEQARIA
                     KTLAFMVDDSAILIVAAGDAKIDNRKYRQLFGTKAKMIDKDLVERYIGHNIGGVCPFG
                     INENVRVYLDVSLKRFTTVYPACGSSNSAIEMTCQELEEIASAEKWIDVCKTEV"
     gene            complement(12521..12694)
                     /locus_tag="PLQ30_01650"
     CDS             complement(12521..12694)
                     /locus_tag="PLQ30_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01178.1"
                     /translation="MKTGTQNGSNNGLFVVQKPRLNLIMMPFFFESRHDLTKVTHSEI
                     GKMIKKDLEKYLI"
     gene            complement(12826..13710)
                     /locus_tag="PLQ30_01655"
     CDS             complement(12826..13710)
                     /locus_tag="PLQ30_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HPW01179.1"
                     /translation="MNNQSDEKLLNDVLRFQGEEITGSLLYEKLAKECKDEHNAAVLF
                     DMAEAEKRHYAFWKSISGQEVAPSRGRIMFFTLLAKVLGLTFTLKLLEKGEEVTAADY
                     ARVASIHPGAKKMGEEEEQHEDALLAMIDEERLSYVGSIVLGLNDALVELTGTLAGLT
                     FAFQKGKLVAVSGIITGIAAALSMAASNYLASKADNDPKAKKAALYTGGAYLITVILL
                     VLPYLIVPQTGFWIYASLGITLLIAVGIIAGFNFYVSVARSEAFKPRFLEMAGISLGV
                     AFLSFLVGLLVRSVVGAE"
     gene            complement(14245..14601)
                     /locus_tag="PLQ30_01660"
     CDS             complement(14245..14601)
                     /locus_tag="PLQ30_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPW01180.1"
                     /translation="MRYCWTTIIVKDLERSLAFYCDIVGLPIVRQLSGAHGNIVFLGD
                     GDTNIELIENPHAAVVTGNRISLGFAVDSLDAMLEFVASKKIDVESGPFRPNEHIRFF
                     YVLDPDGVRVQFSESM"
     gene            complement(15495..15568)
                     /locus_tag="PLQ30_01665"
     tRNA            complement(15495..15568)
                     /locus_tag="PLQ30_01665"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15532..15534),aa:Val,seq:tac)
     gene            15763..18111
                     /gene="lon"
                     /locus_tag="PLQ30_01670"
     CDS             15763..18111
                     /gene="lon"
                     /locus_tag="PLQ30_01670"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPW01181.1"
                     /translation="MSASELIPIDQILPGKLPVITLIGKPIFPGIFTPIMVGKEPDIQ
                     LVEQAISADGMIGLVLQKDDSDEPSSDNLFHVGTAAKIIKKINLPDGGINIFISTFKR
                     FKIKKFISKEPPIITAVSYLDDENYDNDEVKALTRALISEMKQLSENNPLFSEEMRLN
                     MINIDNPGKIADFITSILNIEKKDQQDILETLDVRERMEKVLIHINREKELFRIQKKV
                     QAEINEKIEKSQREYFLREELKAIKQELGMAPDARSSDYQRFREKIDSFQFEGEIKEI
                     VEQELEKFNLMEPSSSEYMVTRNWLDLVCSLPWNTSITADFDIKHAKKVLEEDHYGLQ
                     DVKDRIIEYLAVRKLKKDTRGTILCLVGPPGVGKTSIGRSVARALGKQFFRFSVGGMR
                     DEAEIKGHRRTYVGALPGKIIQGLKIAKSRDPVFMIDEIDKMGISYQGDPSSALLEVL
                     DPEQNFSFRDHYLDLPFDISNIFFIVTANTLDTIPRPLLDRMEVIQLPGYVDVEKIEI
                     ARHFLIPRSLERNGLGKNQVKYTRGALLAIIDGYAREAGVRNLEKSLDKIHRKIAKSI
                     VIENRQEEEFKIDKHAVEKYLGKPIFRDEELKRASKPGMAIGLAWTSLGGDTLLIEAV
                     ANPGKEGFKLTGQMGPVMQESAGIAYTYVRNIAAQKYGIQAEFFESRQIHLHIPEGAT
                     PKDGPSAGITMATALLSLVINKRIRDRLAMTGELSLTGQVLPVGGLREKTVAAKRNKI
                     REIIIPAANEKDLDEIPEHVKKGLVFHPVSRMEEVIEIVFGS"
     gene            18126..20324
                     /locus_tag="PLQ30_01675"
     CDS             18126..20324
                     /locus_tag="PLQ30_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPW01182.1"
                     /translation="MALLPFFCGKDCGGDACPLLAEVEDGHIRGMHLNPLAGRYIKPC
                     GKGFRAHKNHASPRRLKTPLLRTGRRGSGQFREIGWDEALSYIVQRLIDIRAKDGPGS
                     VLCIASSGSTGALHNSETLARRFFNSIGGCVNVKGNYSCNAATYALSRLLGASRMNSG
                     FDAETIRYSNLIVLWGSNPFEARLGAELPAYLIEAKKRGARIIAIDPRRSISARILNA
                     EWIGIIPGTDAVLGYALLFEMLQDARFDAEFISKYSIGFESLTDYVLGKIDGIPKTPL
                     WASSICGVDANVIIRLASFFWDEKPAMIIPGYSIQRVAYGEEAFRLTMAIQLATRNSG
                     LLGASTGSINNCLPGIKVGTLKELPSSLTKGAFIKEGFTKEAHLWEDNHKSQGIEAEL
                     FTKTVPILRWADAVLSPSSYGLGSIKALYSAGGNFLNQGANVTKNIKAFESVEFAVCH
                     ELFLTPSARYCDIVLPVSDGFEKEDIGIPWAGNYVLYKPEICRAFPGTHSDYWIFSQL
                     SERFGLGSVFTEGRTESQWIEQFLAESEIADIEAFRIEGIFLKKGLLRGGLDDFIADP
                     VHCPIGTNSGKIEFASPLWNQNMTLHWQGLMKKGSTEESFREFRLLTPKVSNFIHSQR
                     IGQEDRSECAQIHINPKDMSYIGAQDGEILKVWNKNGTLMASALADDWVQPGTVWIEE
                     GIWTMPIDEVDLCGNPNMLTSDEGTLESISNIMHGIPICIGRIPEATIGH"
     gene            complement(20281..21894)
                     /locus_tag="PLQ30_01680"
     CDS             complement(20281..21894)
                     /locus_tag="PLQ30_01680"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HPW01183.1"
                     /translation="MKEQDLHIELLADMKKKNIDITLTLDILNRYNAGQFDHYEKVIP
                     SGVPPLDGIHVIDIRNRSPNNILFKYPKQESILNLEHNDIFLHSAFPAGNKNIFEIRE
                     MGREMLCFTENALEEIGKHLLPKTAYGVLNGGSATSYIDIKKNGSIDESLFEAVRLTF
                     EDLAKEIENKPKGLTPAYINPDGSAGASFLELKMRSRLLIAKELGHGRLSRKPGALAS
                     SAYQKQPDFLPLFQMTSVGNHEELLRAYEEMRVSPYLSDLAHVVGLEPCEWVSGIQPL
                     IAAYTHSAEGLPKRVFDRAYGKHNSSLAIPGGHGQSFMILADIYRTLLKQGMRYAMLS
                     NVDNTGAYLDPIELAILAISGKPAGFDFAFRTPVDVKGGILILAPNGSRNVVDIGPAI
                     GLEEVECLEKSGSAILFNCATGIFDLEWLVPHIEEISRALPIRFSDQSKDAGNYSQAE
                     QVTWEVVSLLPDFIAFAVQKDRRFLAAKMLIEMLLASGFGIDKPSIDPSLKQLGMTMH
                     NGQSWLLENVYGLTKVNGRWLPQESALCI"
     gene            complement(21906..23000)
                     /locus_tag="PLQ30_01685"
     CDS             complement(21906..23000)
                     /locus_tag="PLQ30_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HPW01184.1"
                     /translation="MNKLFLVGDVGGTNTNLALARHDESGIELIEKAQFSTQNEKSLL
                     DPIKRFLESRRGGGANHKIDFCCISGAGPVQANNSIQLTNAPWGIKASELYEFLRVPV
                     KLINDFTALSYAVTILDHKDKDKVTQLPHPDGSIYEPQDGIMLIIGAGTGLGVGFVDR
                     RGDSVMVFPSEGGHSELSCFDRLSDSFYAWLRKKYDYVPGAELAISGQGIANIFEFIC
                     TEDLAMSIVEGDYEISQKALEKGLSDTARNILDLPRFEWPALISQNARSDAFCSLTME
                     LFVRLYALKAANLVSIFLPAGGVWLAGGISSKNEDWLIEKSRFMRWFERNYATHIRDI
                     LCRTPVLIVKNYDISLMGAAIAALQFSTHH"
     gene            complement(23022..23825)
                     /locus_tag="PLQ30_01690"
     CDS             complement(23022..23825)
                     /locus_tag="PLQ30_01690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPW01185.1"
                     /translation="MDLDNRAMASLFLPSSGHVLAMLIDDQDSEEYQNLDGISLPARL
                     SYDRIAALHPEMNALRPRYEAFIDVFYRRLLESGISDFPAKWFEQNAQTWNGNVTLPG
                     LSSLPRLDELDYSHTFALDIFLQDVEYLSWNIQRGPVIHSLSNGIVVAAESGWLGFPR
                     KSAALSYIKGGISPKSGNGVIVYSPEEHRYYLYFHLYDLFVKKGDILLKGQAIGHGGN
                     SGINARKKGGGDHLHLEIFDSKSGRFLRNTQIIALLKDSSKNKTAVSPK"
     gene            complement(24167..25915)
                     /locus_tag="PLQ30_01695"
     CDS             complement(24167..25915)
                     /locus_tag="PLQ30_01695"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPW01186.1"
                     /translation="MNHEEIILRAQEYIANETDPSFSEEIKELLQKGDWKELEDRFYR
                     DLEFGTGGLRGIIGGGFNRMNTLVVTRATQGLCDYIKEQFPQKQLSACIAYDSRRKSK
                     EFSLATALVFAANDIKAYIFPELKPTPVLSYAIRKLGADTGVVITASHNPPNYNGYKA
                     YWNDGSQVVPPHDSGIIEKVLKAKSAKQMSKTEARSKGLLVEISQEIDDDYVAMVKSH
                     LLRSYLFSEMGKSVNIVYSPLHGTGARLLERIMKELGLNVLTVPEQREPDGEFPTVSY
                     PNPEESAALAMAIELGKKTHADVVMATDPDADRLGIAVPDKAGEFVLVTGNQLGSLHL
                     DYIALTLKEINSMPPRPAAIRTIVTTELQKAIAEKYGIESFECLTGFKWIADLMRRFE
                     TENIDFIYATEESYGHLIEKEVRDKDGISAAALTAEMTLYWRSQGKSLLDRLEDLYKE
                     HGYYEEKGLSFYFEGEQGMRIMNSIMEDYRKQQPDQFGELAVICTRDVKAGTEWDRDG
                     RIRKIDLPQSDVIQWRLEDGTLLTVRPSGTEPKIKYYILCHDQSSELSLSKEITQKKI
                     ALIAEAITAMVDSHRK"
     gene            complement(26056..26727)
                     /locus_tag="PLQ30_01700"
     CDS             complement(26056..26727)
                     /locus_tag="PLQ30_01700"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HPW01187.1"
                     /translation="MPKLQPELIIFDLDGTLVDTIVDIASSVNYVLSQFGLPNHEISD
                     YKQMVGNGFRVLVQRVLSPEKSNDDIFFDKILRLSMQRYEEHALDRTRPFPGTVETLE
                     SLYRNEIKLAVLSNKPDHLSKKIIESLFASVKFVAVWGDNKDRARKPDPSATLELCRN
                     ARVDPQKSLFVGDSAVDIQTAKAARIAAVGAVYGYRSREELELAGADYLITSPPELLS
                     IVEFK"
     gene            complement(26766..27116)
                     /locus_tag="PLQ30_01705"
     CDS             complement(26766..27116)
                     /locus_tag="PLQ30_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01188.1"
                     /translation="MDFSEKLRKFMENSAEASREFLEKAADQAQVWGEMGRLKIEIIQ
                     LRTKAQSLTAKLGTEVYNLLVEKNEPMIGSSTPEIEPIIRELQNLDRLIDEKENLYRS
                     KGGKDSDLNIKSKE"
     gene            27324..28238
                     /locus_tag="PLQ30_01710"
     CDS             27324..28238
                     /locus_tag="PLQ30_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HPW01189.1"
                     /translation="MLWIYTIFAVLSAWWVVFIIIAAIRKQKPVFLFLLILFLVWGIS
                     EIILMMSSPPIAAILFIVPFLILIQMMRVVQEYQRGVLFRFGRLQKVMEPGFNLILPF
                     GIDSLRKVDMRTFTIDVPKQEVITRDNVPVIVDAVVYFNVFDSMLAVVKIADYTKGTS
                     LLAQTILRSVLGQHELDEMLTQRAQLGQVLQGLLDDATNPWGIRVSAVEIKAIELAET
                     MKRAMARQAEAERERRAKIIAAEGELQASEKLAQAADVLAKSPASLQLRYLQTLSEIA
                     VEKNSTIVFPLPMDLLNVISKVAEKSSE"
     gene            28235..28702
                     /gene="nrdR"
                     /locus_tag="PLQ30_01715"
     CDS             28235..28702
                     /gene="nrdR"
                     /locus_tag="PLQ30_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPW01190.1"
                     /translation="MKCPYCGSPEDKVIDSRTLANGEAIRRRRECISCGLRFTSYERI
                     EEKPLLVIKRDGRREPFERQKIERGLVRALEKRPISQMSIENLVNEIEDNAAEHAKAS
                     NEISSEELGRMVLQKLYSLDKVAYIRFASVYRKYDTLDEFIREIERLENGQTG"
     gene            complement(28727..30823)
                     /gene="fusA"
                     /locus_tag="PLQ30_01720"
     CDS             complement(28727..30823)
                     /gene="fusA"
                     /locus_tag="PLQ30_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPW01191.1"
                     /translation="MSYTTEQIRNIAIIGHGSTGKTTLLEHILFQGGMISRPETVDSG
                     KTVSDYSEDEIARKISVRSSLTHVNTDGYKINFIDAPGSGDFVGEAILAIRSTEAALL
                     VIDGKSGVQIETVKLWRILERHKRPRMMFITRLDEDRADFSNALADIKEKFRVTPVPV
                     TIPMGEGFSFKGIIDVLNNKAYMRPVSHDQKEVPSEVPPEYSEAVESARALLSEAAAE
                     GNDDLMEKYLLEGELTQEETLLGLKEALAEGKIIPAFAGAGFVNSGTAALMDFIFQSA
                     PSPFARAPEKAKDQDGNDVEISIDPLKPASAYVFKTQIDQFSGRLSYIKAITGTILSD
                     IEMVVSRDLHRERIGKLYTMQGKKLEEIPSLPAGDIGVLAKISSLKTNDTISQFDKPI
                     SYEPLQLPSPVHMLAISAVNKKEEDKLNELLNRAAEEDLTFRVSYNPETRETVIAGMG
                     EQQINMILDKIKSQSKIAAETRIPRVAYRETITKKATAEYTHKKQTGGHGQYARVVFE
                     IEPLERGKDYEFENRIFGGAVSKGFMPGIEKGIHQAMEAGVLAGYPVVDTKTAIIDGK
                     EHTVDSSEMAFKLAARGAFREAMKLASPVLLEPIMNLSVFVEDKYLGDVMSDLSGRRG
                     KISGQNPIGGGIVQIDAQVPQAELLRYAIDLRSMTSGTGSFEVEFSHYAPISGKIAED
                     VIKAAQAFKIQEAEEE"
     gene            complement(30941..31735)
                     /locus_tag="PLQ30_01725"
     CDS             complement(30941..31735)
                     /locus_tag="PLQ30_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPW01192.1"
                     /translation="MDSILDALQEGRLFELPENDKDHALQFLAHIIEAFPQIPTGTDI
                     VGNVMAREKSMNTALGRRWACPHARVEFEEDLMCVVGWSPTGIDYGAPDGRPVSIIVM
                     YLVPSNQRNHYLREISTLAKVLKSSPEIDKLSTIRELNDVRNYLLDLISVSKDTVGPD
                     ARARMIRLQAKAAMATQPVAELSNLIIEPVSIIAGMGLKPLALTQNLELMNFIETTAG
                     LTERIETEGKYQNGLWRIIRRSATAYQGGRTVFDCIALMPASTGQR"
     gene            complement(31738..>32189)
                     /locus_tag="PLQ30_01730"
     CDS             complement(31738..>32189)
                     /locus_tag="PLQ30_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HPW01193.1"
                     /translation="DIDNIKRFMMCIGPISSIFDYATFALMWFFFNAKDYLNPALSLA
                     QRDAAASLFQTGWFVESLLTQTLIVHIIRTKRIPFLQSRASAPMILASLLVMGIGIWL
                     PYSPIAGILGLVPLPYIYWLWIAAFLLTYSIITHKVKCWFLKHYEGA"
BASE COUNT         8959 a         6947 c         7325 g         8958 t
ORIGIN      
        1 accaccctct agtttcccta gccctatgcg tcaactcatt cctgacttgt acccatagtt
       61 atgaaatgat agtatcacgc cgatgatatc cgttttttct ccttatatta cccgtagaga
      121 catggatctc gttcttagca gaatggtgga ggatgctgta agcgaagatg tatttaacag
      181 gcgctttgaa aatgccgtaa aagagcagtt taatcttgaa tatgtgatat caatgagaag
      241 cccttataac gctctccaaa aggcacttca agtttttggt ctggaaagag gtgcaaagat
      301 agctatttcc gcgcttgcac cagcatggca aaaggatgcc ttagaggaca gtggattcac
      361 accggtaatt ctggatatcg atcaagaaac atttcatcca aaaccggaaa gcatttccct
      421 ggccaaccca tcggcgatta ttctgttcga tgcccttgga aagtcccctt ctgaagctct
      481 gttagaaaga atcgatatac ctattataga ggacatttcg cagactttgg gtatgatcaa
      541 agtgtcagat gaaaaacatc tatatgggca ctttctgatc tggagtctag agtcagattc
      601 tgcaattgca actggaggag gggctcttct ttgtgcgagc agcaagagag acgctcaaat
      661 acttcgattg ctggatgaat ctatgccaag agagctaaag atgaccgact ataatgcggc
      721 acttggcata tctcagatga aaagctatcc taagatgata gagcgaagaa agacaatcta
      781 ccggagattg gtagggcaac ttttgcggac tcggcattca tatttgcagc aagaggactc
      841 tgagtcctgt cccttatatg cctttccgat atttcctgaa acaagcgtta aagagattat
      901 agactatgcg atgaagcatg gtgtagaaag caagcatgca tttgcttctt gcatatccca
      961 gagattatat gagagcgagc ccgaacaaga agcggtattc ccaatcgcac ggtcggtagc
     1021 attgcgctgt attttatttc ctatgcatca taaactcaca aaccagcaag tagaacagat
     1081 aggcaaggta attgccacac taccatagga gattcttatg tcggagagca aatccacact
     1141 aattttcgca aatttgaaca aagatgatgc aggtcatgca gcacaaattg tgcaagctga
     1201 gctggaacag aatggctggc atacagaaat cctggcattt cgaggctcaa tatcggaata
     1261 ccctgatttt gatcgctata gccttatcgt tagtcttggt ggcgatggca ctcttctata
     1321 tgctgctaga tttgccggtc ctcgcggcat acccatactt ccaataaact taggaacttt
     1381 aggatttata gccgctaaca ggatggaaac ctgggctgtt actttcgaac attggcagaa
     1441 aggagctcta gatccatcaa ggcgcttgat gctgcaatct tatgcgtaca gaggaaagaa
     1501 gattttgcac aaaggaatcg cgctcaatga tatagtcgta tcctctgaga gtggcgctag
     1561 aatgatacgc atgtgtctgt caataaatag agaatgcttt ggttattaca gagccgatgg
     1621 tctcatcgtc tccacaccta ccgggtccac tgcctataat ttggcagcgg gcgggccagc
     1681 cttacatccg gagatcaatg cccttataat aaacccaatc tgcccattta cgttggcatc
     1741 caggccttta gttatacccg cttctaattg catagatatt gaaatcgatg agaccaagaa
     1801 agcaggagct cttctgatct cggatggtca tatagtggca aatctcgaga agggcgatgt
     1861 gatccggatt cgaaaatatg agaagagtgc attcttaatt gttccttcag gcaatgcctt
     1921 tttctctgcc ctaaggacaa aactgggttg gtcgggagac aacgatgctt gagcagcttt
     1981 cggtgcgtaa tttcgccatt attgagagca tagatataga tttatctgcc ggaatgacgg
     2041 tgttctcagg agaaaccgga acaggcaaat cattgatcgt cgatgctcta ggatttttgc
     2101 ttggcgcacg agtcgacaat tcaataatcc gcgaaggagc cacagaatgc tccgtatcag
     2161 ggcttttctc tgcttcgagc aatgctcagg tcgggctctg gcttagagaa aaaggcatcg
     2221 aatggcagat caatgaagga attttgctgc gtcgaattct caaacagaat ggcagaacac
     2281 tggcatggat tcaagatcga caggtttcca ggaatgagct cctggaattc actcagtacc
     2341 ttgtagatat acacggtcag catgaacatc aaagacttat tgattcgtct actcatctgg
     2401 acatgttgga tgcatatgct aacctagagg atgaattgca atcgtatcaa gtgctgtatt
     2461 cggaatggag agaagcgact cgtcaactgc aggaagtact ggaacaaaaa gcaaagcgct
     2521 cgcaagaagc tgattatctc gattttgtaa ttagagacat tgcatcgata aagcccaaac
     2581 ccaatgaaga caatgagctt ttgactgagg aaaaggtact ctccgagcac gagaagctat
     2641 ttggagcagt atctgaggca tcagtgcttt tggatagcgg ggaaggtggc gatgtgctcc
     2701 ataccctaaa aaggattcga gcaagtatcg aaactgcgcg ctcagtcgac attcggttag
     2761 gatctttctc tgaaaggctt tctgcagcct actatgagct ggaagacata tcagaatctt
     2821 tgaataagta caaggccggc cttcgatttg atcccgatcg tctggaaaaa atcgaaagga
     2881 gactatccga ttttcaacgc ttaaagcaca aatatgggcc aaagctcgat gatgttctgc
     2941 agacatacga cagggcaaag agacgactcg aagcgcttga acatgctgag gagaatgctg
     3001 aagaactgga aaagaaagaa aaatcgctcg agaaagaagt gttgaataga gcgtatcagc
     3061 tgtctgaaaa gcgaatgatg gcagcggttt caatgtcatc ttcgataaga gagatagtta
     3121 aagatctagg aatgccagaa gcgcaggtaa accttcgtct gaagagaatt gtagacgaga
     3181 gaggaggaaa tagggcaacg cttcgcggca tagatgaagc ggaattcttc attgctccaa
     3241 accctggaga accggaactt cccttgtcta gaatcgcttc aggaggagag ttgtcgagat
     3301 tcgctcttgc aatcaaggct gtattagctt ctcatgatga agtagagacc ctagtgttcg
     3361 atgaaatcga ttcaggtata ggaggccaag ttggtgttgc cgttggcgaa tacctgaggc
     3421 aattgagcca atatcgccag gttctatgcg ttacacatct tgcgaccatc gcggtgcgag
     3481 ctgatcacca aggcaaagtc gataaatttg tatcaaaagg cagaacaggt gcctccatac
     3541 gctttctttc tggagaagac cgggaaaccg agattgcacg gatgctatcc ggattttcag
     3601 aaagcgaagc ctccagaatt catgctgctg aacttttgag aaaagccatt tcacataatt
     3661 gaatagttta aaaagagaac atgcgagcct attttcatag attataggtt tcaggaactt
     3721 ttcaattcct agcctcgaca ggcaagctca tgaagagcat catgaggcaa aagctcaccg
     3781 acagtcgaac ttatatatga atcttcgctg ttgccgaaga ttaccggagc tgaggagtgc
     3841 atgaactctg atatcacctg tcggcacgct ccacaagggc tgacagggta atctgcatcc
     3901 ggcgttgcta tgactagtgc tttaaatcgc ttcttgccag aagcgatagc cgcggcgatc
     3961 gcattgcgct cggcgcaaat tgtcaatcca tagctgcggt tttcaacatt gacgccggta
     4021 atgatgctat catcgtcgca gagcaatgcc gcaccaaccc taaacttaga ataaggggca
     4081 taactctgtt cacaggcttt gagagcatgc ttgaatagat aagtatattc cgttgtcatg
     4141 tcagttaaga tccttttcca ataatatatc cgaaaatgaa cagaattgaa atgcgccatg
     4201 cggtattgac tcaaagactt cagtcatgca ccataaaata caatgagaag gcgattttat
     4261 ggccttatta ttggtatagc tgcgattatc tttacgctct tttcaataag aagcactttg
     4321 ccggagagag agatcacatc aagcgttttt ttgacatttg ctgaggctct ggatgtgtca
     4381 agaattccta aagcaagaag cgatcagatc gtgcccttta ttgcagaatc cttcttaggt
     4441 tatgccgatc cattagaaga gctgctctat gtccatcctt tcaagggaag agcagcaata
     4501 gatagactcg gttggatcac atatgatcgg cttgctaccg atttgaaatt atatgacctg
     4561 aatggtggtg ggcagcaatt tcaagcatat gcttatccat ggtttaaggc ctcatggaga
     4621 gtattgcttc gagccgatca gatgggcata gcggtaatgg acaagacagg attgattagg
     4681 tgggagaagg aatttccgac gccgataacg gcgatcgatg catcttctct gttcctcgcc
     4741 gttggaacac ttgacggatc gatcagcgta ttcgatccaa atggatgggc tatcctttca
     4801 atgcaaaata gtcttcaaga atttcagaca atctatggtc tagccctatc tgcggacagc
     4861 aactatctca ttatcgtaag aggtatctca cccaagaaaa ttgaaaccta caggtttaca
     4921 gacaattcct ttgcaaaggt atccgagtct tcgttgatgg taaatacgac tttccaggca
     4981 accatagcaa tagcccggga caattcacac gcaataatga gctttggtga tatgataata
     5041 tattataata taaagaataa atattataga attcttgaag caggaatggc aggtgtagag
     5101 ttatcagaga ctcaggttga ttttaggtat tttgtacttg gatccttaaa agactcgctc
     5161 gtggcgatcc tgagaagcga tctggaagga aaagcaaaat ctgatgttat cattcttaat
     5221 catggccttc cagtacatat tgctcccgga gcttttggcg caattggcga tgaagaagta
     5281 atcggatttg tatataaaga tggcctggaa ctattaaaag gatggcggcc atgaagggga
     5341 aatatccttt tttaataagt tatttatggc tcgctacctt gcttggtgca ttggacttca
     5401 agatacctga agttattgat ttatcagggt ttggaacagt ccttggcgat caagctaagc
     5461 aaggggtttc gttagaatat gatgtaagag aaagatgcta tggcaagatt ccaagaagcg
     5521 atgttcagct gatatcgtta gatacaacgt ttgaagcacc ttcaagcggt gctatttttt
     5581 attcgcaagt tgaagcagat ttgccaacag tcttctcttt tccattaggt ggagccctgg
     5641 ctgttattca tcccgataga tttgtgagca taacttcagg gctcgatcct attcaggtca
     5701 taaaatcaac ttcccgatca tcgaaagaag tccagaatag ctttgcaata aagaaaggag
     5761 acaagcttac tggaggagat ggctcgggcg tatacccatc caaaaatttc ggtgttagac
     5821 ttttcgacgc aaagaacgaa ttatggatga atacgatttt tcttgctccc tggatcaggg
     5881 ataatacatc tccaatcatg cgcagcgcaa aactgatcat gactggcaca gataagaagg
     5941 tattctttga tataatggag acgaataaaa aagaaaaact tattacctgt cctcagggaa
     6001 attattcatt gtttgtcgat gcgcgagata tcatcatcac aagttcgcgt tactattctg
     6061 ctccctattg catcaaggtt acgcttgatg gggaaaccgt aatcgatgct tcattcatag
     6121 ccgcgcgcgc aagcggagaa gggatatcat ttctgggcaa tccacccccc tcgcgaggat
     6181 ccataggaga aggttattca ggagagggtt attcatacca tgttggtcaa ttcttgattc
     6241 tcagaggcaa acacgatctt atgcttcaag tgaatgattt tgcggggaat cagactacta
     6301 tgcgaacttc cataaccgca tactagaaaa gcttgccccg atttcggttc gctgatagga
     6361 ttctagatat gtcgaataat agagcgacca ttgctgataa tgtgactatc ataaaagaac
     6421 ggatttggcg cgcggcagaa aaggccggga ggtcttccga taaaatcgat ctcatggctg
     6481 tgaccaaatt ccagcctatc gatgttattc gacaggccta tttggcaggc attagaatct
     6541 ttggagaaaa cagagtacag gaagcatctg cgaaatttcc ttctctggaa ccagagctag
     6601 aaggtgctag cttgcatatg attggcacag ttcagaagaa taagatcaat aaagctttga
     6661 caatcttcga tgggattcag gggatagacg atatagaaac tctagaggct atccttataa
     6721 gaatcaaggc cccaaaaaga ccaataaagc tatatctgga gctgcatacg ggagagattt
     6781 cgaaatccgg cttccctaat atagatgagc tcctgcgagg gtgcgaggtc tatgcaagat
     6841 atcgtcaatc agatcctcag tggatgacaa aagccttgct ctgcggtctt atgaccatgg
     6901 ccccttttac gcaggatatt tcattgatcc gtagatcctt caagatgctc tccgctgcaa
     6961 aggacaaaat aaagagcagc tttgattttc cagaatttag cgagctttct atgggcatga
     7021 gcaatgattt tgagattgcg atagaggaag gttcgacact cgtgaggata ggaactgcta
     7081 tctttggtga gaggaacgca taaaatgaat aggcgctgcc ttttattgct cattgttttt
     7141 ctatgcggag acctcttggt ttattgtttt ccagcacaag ctcaagaaga aaatcctggc
     7201 tcagaaattg tcgctaaaga agaagagaat tctcaagagg cgtcggaagc gtctgtagct
     7261 ccagaggcgc ctgcagctcc agaaatttca gaggcgtcgg aagcgccaaa agtgactcta
     7321 ccaacttttt cttatagtga cactaaagaa ggcatgttta ggcgcagcct tgtgatctct
     7381 attgggcttt ttcctttcag ctatttctat actaattttg ctttcgattt ggctcgctat
     7441 atatcgcatg gcttcgatac ggcttacgcg ccttggccat tcaataccca atatggtgtt
     7501 gccctgacaa atagcgaagt atggacaaga atcggtatag cttccggagc aagcatattg
     7561 cttggtttct tgagcgttat catagagtga tatacaaaaa gcactgtatt ctgtatactg
     7621 cagcgtaacc tttctataag gtagcgccat aatttccatt tttgagagag gctaaatatg
     7681 gcgaatggca gcgattttag aggcgattat caagaattca acctcgatga tgcaatccgc
     7741 aaaattccgg attttcccaa accgggcata ttgttctatg atattacaag catccttgct
     7801 aacccaagag cattcaaata ttgtatcgat tcaatgttta agctatatgg agatactgaa
     7861 ttcgatgtgg cagctgctat tgaatcccga ggatttgtgt ttgccgcacc cttctgttat
     7921 gagcggaata tccctctgct tttagttagg aaaaagggca aattgcctgg aaggaccatt
     7981 tcctgcgcct atgagttgga atatggaact gccgtgttgg agatgcatat tgaagatatc
     8041 aagccaaagg ctcgttttct gattatcgac gacctcatcg ctacaggagg cactattaac
     8101 gctacctgtg aaatgattaa acgcggtggg gcggtgcctg ttcgcgcgtt ctcagtgata
     8161 ggcttaccat ctttgaaata ccaagagaaa gtgacagatg ttggtataga tacactcatc
     8221 gagtattctg gagaataggt gagtaaaaga agccctttgg ggaaaagagc tccgaatttg
     8281 gtttgagcca ggtaacaaca gaaaaaggag caatctatgc ttgaacgatt aaaaaaaaat
     8341 cctaattgga aaaatagaga aggtccatta gtccttgtga taatggatgg agtaggttac
     8401 ggaacttaca aagaaggtga tgcagtcctt gcagcgagaa tggatcattt cagggagctt
     8461 gaagccaact gccccaaaac gcggcttaag gctcatggaa ctgccgtagg ccttccctcc
     8521 gatgatgaca tgggaaactc ggaagtcggt cacaatgcga ttggttgtgg tcgagttttt
     8581 gcccaaggtg cgcgcctcgt caatatttct attgaatcag ggataatatt cgaagggaaa
     8641 gtctggagag agcttattgc caatataaag gagaccggag gaacactcca ctttattggc
     8701 cttttctcgg atggtaatgt tcactcgcat atcgatcatt taaaagccat gctcgttcaa
     8761 gcacagagag aaggtatcaa acgagcgcgg atacatatct tgctcgatgg gcgagatgtt
     8821 ggaagccaaa gtgctcttga atatgtcctt ccttttgaga cttttcttga ggaatttaga
     8881 aatcaagggt cggattatcg catcgcctct ggaggtggaa ggcaatacat aacgatggat
     8941 cgttatggtg ccaattggca gatggtcaag cgcggttggg actgccatgt tagaggcata
     9001 ggaaggcaat tcgccagtgc ggaagaggca atctcgactt atagaaagga gattccaggc
     9061 attattgatc aagatattcc cgagttcgtc attgccgaaa atggcagacc catcggtaca
     9121 atcgaggatg gagactcggt tatattcttc aatttccggg gtgacagggc gcttgagatt
     9181 acagcagcct tcgagcagga taatttcgac aaattcgata gagtcaggcg accaaaggta
     9241 tattatgcag gtatgatgca gtacgatggc gacctcaagg ttccttccaa ttttctggtt
     9301 tcaccacccg ctatcgacag aacgctcggc gaataccttg cttatacggg cttaaagaca
     9361 ctcgctattt cagagacaca gaaatatggt catgttacct attttttcaa tggcaacagg
     9421 tcagggaaat tttcggaaga actggaagac tatgtcgaaa tacctagtga tcgtgtgcca
     9481 ttcgagcaaa ggccatggat gaaatgcgca gagatagccg attatgtcat aaatgcaatc
     9541 gaaaatggcc catatagatt tattcgcctc aatttcccaa atggcgacat ggtagggcat
     9601 acaggggtct ttcaagctgt ggtgtgtggt ctggaaggaa tggatatcca gctaggcaga
     9661 atatatgagg cagtgcgaaa aaaaggcgga atcctcttga taacggccga ccatggaaat
     9721 tccgatgata tgtatgagca cgacaagcag actggaagag ttattttagg tcccgatggc
     9781 aaaccaaagg caaagacaag ccattcgctc aactctgtcc cttgcgtaat atacgacccg
     9841 caatatcaag gcgagtacaa aaaggagctt cggcaaggaa tgggaatatc aagcttggcc
     9901 gcaacctgtg ctgaacttct gggttacatc ccgcctgatc attatgatcc atcggtgttg
     9961 gacatgccta tagagaatga ggcatagcag aataatgcag aatccagaat atagccataa
    10021 cctggagata gaacttaagg cacgagttca gcagagagag gaagtggaat ctcgcctttc
    10081 cagttttatg cactatatag gacccatcaa taagctagac gaatattggg aagtatcaaa
    10141 tgcaagatta ggggattttg gtggttttag atttcgcgtt cgccatgaat tggctagtac
    10201 tactataaca ttcaaagaaa agaccttcga tggagacgta gaaatcaacc atgaattcga
    10261 gtttagtgtc aatggtgaac cagcttttaa ggccttcata gaaaaaatgc acgcacatct
    10321 gatctaccaa aagcaaaaga gaggtacccg ctgggaggat ggtacaggct tggttgcgga
    10381 ggtagtcgaa gtatcttcgc tcggtctttt tcttgaagtt gaatgcattg gcgatagtcg
    10441 cgtcacattg cagcaagaag aggccaaatc ccgcttatat agggttatcg atcaatgtgg
    10501 catttcaaga agcgctcttg agccaagacc gtattcgcag ttgctagggt attgaaagtt
    10561 gaataaacaa gaattatcgc gctgacggac ttgaatcccc gacctaccgg atgaaaatca
    10621 caagttgcac aaggtatata taaaatattg cattatatgc tcaaaactag cttcttacta
    10681 attaataact aatgattgtg tccagtcctt gttgatagtg tccagtcttg ttcgcgattt
    10741 actgctaaaa agatcattag gaatcgtctc cattacgcta ctgtatataa taacgcctga
    10801 acgagtcctc aagaccgtat tagatttatt gtcttccgcc aaactcacgg cttatttcca
    10861 tatagccggg gtatgggatc atcgctttac caatagaaat agaaggcttt aactggaggg
    10921 ttagtgtaga gggtatttcg gatgcaccag ctaaattaaa caacaaattc agaatagcat
    10981 cttgtgtatt gtcttctttt aaagaaaaaa gatcgaatgt cacttttaat ggaatgactg
    11041 caacacgatt gcctccagga acctttacag aatcggtagt gaaaccacta aataatggtt
    11101 ctccatcaag ggctacagca taatctacac ggtctactgc tacagtctgg gtattcggat
    11161 ttcggatttc tacagcaaga gtcatggtta ctggcaaaga acctttctta atggctagag
    11221 taattctacc agcatccaaa aggctcaact cagaaattga tttcttacca ctaatattca
    11281 ctcctgcagc aacaacatcc ttaatcccct gcattcgaaa ttcacatctc ttcagtgtag
    11341 taacatcgct tacggtagca caacttgtca tcacaagaat actgataagt atgacactag
    11401 cctttttcat acaacaaacc tccagatata tatatataat atttttcgcg ctcaatttta
    11461 ggcattgtca atcgttgaga atgtaaaata ccggattcag gcgcttttta ttatagcctt
    11521 ttctttcata ctgccttgtt ttccagccaa ctcttataag cccttatctc ccgctcaatc
    11581 ttcgagctag tttctttgat gattcttccg cggaaatagg cagcaggcag gttgacaagt
    11641 gagccaacga agccttttcc gttcatctta tagatgattc tcatgatata acatcttctt
    11701 attcgatccg aatcttattc gagtaattat tatcgatgtt catttcgtcc ggtatgttgc
    11761 ttatctgcct ccaatgatac gatctgattg ttgaagaagt atgcaagcgc tttccaggtt
    11821 tgcttttaca tcttctcgcc ttgcttgttc aacctcacga ccaacttagt gaagaaatca
    11881 tccttcattt atacatgtat tctattatct atcaaggtgt cgagtccctc cctcgttgtc
    11941 ttatagtctt cagacttctg tcttacagac atcgatccat ttctctgcgc ttgcgatctc
    12001 ttccagctct tggcatgtca tctctattgc cgaattgctg ctcccgcagg caggatacac
    12061 agtggtaaat ctcttgagag agacatccaa atacactcgt acattctcat tgatcccgaa
    12121 tggacatact ccgccgatgt tgtggccgat ataccgttcg accagatctt tatcgatcat
    12181 ctttgccttt gtaccgaaaa gttgtcggta ttttctattg tcgatttttg cgtctccggc
    12241 agcgacaatc aatatggcag agtcatcgac cataaaggcc aatgttttgg cgattctcgc
    12301 ttgctcaata ccaagcgcct tagctgccag ttccaccgtt gcgctcgatt catccagctc
    12361 tcttacctgt tcgtctttgt tccacttttt taggtgttct tttgcccttt ctagactcat
    12421 agatcagtat ctctcctgat tgcgattttt acgccgtcgt caatgggcag tatcgcgctc
    12481 atgactcggt cttcatgtat caaaaggttg ttgagctcct ttatataagg tatttctcaa
    12541 ggtctttctt tatcatcttt ccgatttcgc tatgcgtcac cttagtgaga tcatgccggc
    12601 tctcgaaaaa gaagggcatc ataatcaagt ttagcctggg tttctggaca acaaagagcc
    12661 cgttgttgga gccattttgc gtacccgtct tcatctattc gataatctcg gcttccatgc
    12721 ccagccggga tgcggaataa atctcgatga taaggcaatt gcaggatttt atctcgatat
    12781 agtccttgaa cacaagcgag gtcttttgca tggtaacggt taagttcact cggcgccgac
    12841 gaccgacctt acgagaagcc cgacaaggaa ggataagaat gctacgccaa ggctaatgcc
    12901 ggccatctcg aggaaacgtg gcttgaaggc ctcgctcctg gccaccgata cgtaaaagtt
    12961 gaagcctgca atgatgccga cggcgataag gagggttatc ccgaggctag cgtagatcca
    13021 gaagccggtc tgtggcacga tgagataggg cagcaccaga agtatgaccg ttatgaggta
    13081 ggctcctccc gtgtagaggg ctgccttttt cgccttagga tcgttgtcgg ccttggaggc
    13141 aagatagttc gaggccgcca tggacagggc ggccgctatg cccgttatga taccgctgac
    13201 cgcgacgagc ttccccttct ggaatgcaaa ggtcaggccc gccagagtgc ccgtcagctc
    13261 caccagagca tcgttcaagc caaggacgat agatccgaca tacgagagtc gctcctcgtc
    13321 gatcatagca agcagagcat cctcgtgctg ttcctcttct tcgcccattt ttttggcgcc
    13381 aggatgaatg gaagccacgc gggcatagtc ggccgctgtt acctcttcgc ccttttcgag
    13441 gagtttgagt gtaaaggtca aaccgaggac cttggccagc agggtgaaaa acatgattcg
    13501 ccctctgctg ggagcgactt cctgtcccga gatgctcttc cagaatgcgt aatgtcgttt
    13561 ttcagcctcg gccatgtcga agaggacagc agcattgtgc tcatccttgc attcttttgc
    13621 cagtttttcg tagagaagag agccggtgat ttcctcgccc tgaaagcgaa gaacgtcgtt
    13681 cagtagtttt tcatcagatt gattgttcat gacatacact cctcacaatg ttgcaattaa
    13741 tcatacttca tcagccctta agactccaga gtcggaaggg tggcgaaact ccgatcgatt
    13801 gaaaaacctg gtgagagatc acggcctctt atacaattta ctttgattcc gagccattac
    13861 tttgtgtcac atctcgacca tatgatgggt tctgactcag acctgaatca taaggataag
    13921 ttctaagtcg tcagactctt gctatgcatg cactcttgcg tccgttagct gtttctaaag
    13981 gcctatatgt attgatggct ttgtcagttt tgatggctct cagtgcttta ctgataatat
    14041 taccatgcgg agatagcacc aattccaata caggatcatc gcaattcgta tattctccga
    14101 aaatcggcgc ccgtagctag gttgttgtgg aagtgaggaa gccgttttcc ggggatacgg
    14161 agatagcctt gcgccctagc atgaagtcgg gctcaggaac tataagtcag gggccaattt
    14221 gatgtttcta tatgcttttc ctggctacat ggattcggag aactgaacac gtaccccgtc
    14281 tgggtccaac acatagaaaa atcttatatg ttcatttggt cgaaaaggac cgctctcgac
    14341 atctatcttt ttcgaagcga cgaattcgag catggcatcc agtgaatcaa ctgcgaatcc
    14401 aagggagatt ctgtttcctg tcacgacagc cgcatgagga ttttcaatga gttcaatgtt
    14461 tgtatcgcca tctccaagaa acacaatgtt accatgggct ccagagagtt gcctaacgat
    14521 aggcaatcct actatatcac aatagaatgc aagggaacgt tccagatctt ttacgataat
    14581 tgtagtccaa caatatctca ttcttttagt cctcccattc caaggatact cggtgctccc
    14641 acagtagggc aaccatgata catttgaaag aggaaagaat ccggctagat tgcatcgcaa
    14701 gaatctaggc taggccgtac tctcgacact ctacctcaag atgggaatca ggatttttct
    14761 caacaaccgc aggctctctg caaattttgg tgcgaacctc aaggctatct ttaagagact
    14821 catccttggt agagtacctt tttcgattct gggcatggct agtgggagaa taggggtata
    14881 ttctctgaga atactgacta ttcgaatgaa aatgtgcacc gcgcactcga ttatttcctc
    14941 gaatagaatc cagcgctcct tcagcatcta cacgaagctc tgacagctca ttataagaga
    15001 gatacggcct actatagtat gatttgacga actactgctt tgagattcga gtatctcttt
    15061 ttgcttcatg aagtgcgaat cgatgcatct tgataagctc aatggagact attcccaatg
    15121 atctttttag cagcaaattg cgaaaaagtc tggacaaaat ccagcctgta ctcctctcta
    15181 acgaaaatca catcaaaact tatttcctca tctgctttat atctttaatg ctcatgtgcc
    15241 ttaaggtgac tttcgttatt ccgatttctg gaatgatctc aacgccaata gttcttgttt
    15301 ttcctattaa aagtttatca tctcattcag catttggtgg tactcaatag cgcaaaaatg
    15361 aatcagaggc caatgccaat ccataatcca tatatgatcc ccatgttatg aaaaactgtg
    15421 atttagcaat tctctttgtc tttcattccc attgacagtc aaattcggat tatgcgttat
    15481 cttatgattc cttatgggcg ctgacggatt tgaaccgccg acctactggg tgtaaaccag
    15541 ccgctctgac caactgagcc aagcgcccta aaatccattc atatagcggc tgaacactag
    15601 catgagccag ccagatgagt caagcaaaat ggccttaaag gcaatggatt cacagaatcc
    15661 ttgccgaata gccttatttt tccgtacatt tgtaatgcgg tttcttattc cgcaatgcaa
    15721 acattaatat attttcatta taattctatt aggtggtaca atatgtcggc aagcgagctt
    15781 ataccaatag atcaaattct gcctggtaaa ctgcccgtaa taacgctcat aggaaaacca
    15841 atattcccgg gcatctttac gccaataatg gttggcaagg agccggacat tcagcttgtg
    15901 gaacaagcca tatcggctga tgggatgatt ggtcttgtgc ttcaaaaaga cgattctgac
    15961 gagccgtcct cggataattt atttcatgtt ggtactgctg caaagatcat aaaaaaaatc
    16021 aatcttccag acggtggcat caatatcttc atatcgactt tcaagcgttt caagattaag
    16081 aaattcatct ctaaggagcc gccgataatt acggcagtca gttatttgga cgacgagaat
    16141 tatgacaacg atgaagtaaa ggctttaact cgagcactca taagtgagat gaagcagctt
    16201 agcgaaaata accctctctt ctctgaagag atgcgcctca acatgatcaa tatcgacaat
    16261 ccaggtaaga ttgccgattt tatcaccagc atactcaata tcgagaaaaa agaccagcaa
    16321 gacatcctcg aaactctaga tgtccgcgag cgcatggaaa aagttcttat tcatatcaac
    16381 agagagaaag agctatttag gattcaaaaa aaagtccagg ctgagattaa tgaaaaaatc
    16441 gaaaaaagcc agcgagaata ctttcttcgg gaagagctca aggctatcaa acaagaactt
    16501 ggaatggccc cggatgcgag aagttctgac tatcagcgct tcagagaaaa gatcgattca
    16561 tttcagtttg aaggcgagat caaggaaata gtcgagcagg aactggaaaa attcaacctc
    16621 atggagcctt cttccagtga atacatggtg actcggaatt ggctagatct tgtctgttca
    16681 ttgccctgga atacatcaat aactgctgat ttcgatatca agcatgcaaa gaaagtactc
    16741 gaagaggacc actatggtct acaggatgtc aaggatcgga taatagaata tctagccgtg
    16801 cgcaagctca aaaaagatac gcgtgggact attctatgtc ttgttgggcc gccaggtgtt
    16861 ggtaagacaa gcattggtag gtctgtagca cgtgctctag gcaagcaatt ctttagattc
    16921 tctgtgggag gaatgcgcga cgaagctgag atcaaaggac accgcagaac ctatgtgggg
    16981 gctttgccgg gtaagatcat ccaaggtctc aagatcgcta aaagccgaga tcccgtattt
    17041 atgatagatg agatagacaa aatgggcatc tcctaccaag gagatccatc cagcgccctg
    17101 ctagaagttc tcgatccgga gcaaaatttc agtttcaggg atcattatct cgatctgccg
    17161 ttcgatattt cgaacatttt ctttattgtt acagcaaata ctctggacac tattcccagg
    17221 cccctgctcg atagaatgga agtgattcag cttcccggct atgtagacgt tgagaagata
    17281 gagattgcac ggcactttct catccctagg agtcttgaaa gaaatggact aggaaagaat
    17341 caagtgaagt acacaagagg tgctctgctc gcaatcatag atggctatgc tcgcgaggct
    17401 ggtgtccgga atctcgaaaa gtctctcgac aaaatacata gaaaaatcgc aaagagcata
    17461 gtcatcgaga acagacaaga agaagaattt aaaatagata agcatgcggt agaaaaatac
    17521 ctgggcaaac ctatattccg tgacgaagag ctaaagcgcg ccagcaagcc gggcatggct
    17581 attggattgg cctggaccag cctgggaggg gatactttgc tcattgaagc cgtagctaac
    17641 cctggtaagg aaggattcaa actgaccggt cagatggggc ctgttatgca ggaatcggcg
    17701 ggaatagcct atacttatgt aaggaatatt gccgctcaga agtatggaat ccaggctgaa
    17761 ttcttcgaat caaggcagat acacctccat atcccagaag gagccactcc aaaggatgga
    17821 ccttctgcgg gaattaccat ggccacagct cttctttcgc ttgtgatcaa caagcgaata
    17881 agagatagat tagctatgac tggggagctg agcctcaccg ggcaagttct acctgttgga
    17941 ggattgcggg aaaagacagt tgcggcaaag cgcaacaaaa taagggaaat aataataccg
    18001 gctgcaaacg agaaggacct agatgaaatc cctgaacatg tcaagaaagg acttgtattt
    18061 catccggtat ctcgaatgga agaagtgatc gaaatagtgt ttggctcatg atagaataac
    18121 aaaacatggc actcttgcct tttttctgtg gaaaagattg tggaggagat gcatgtcccc
    18181 ttttggccga agttgaagat ggccatataa ggggtatgca tctcaatccg cttgcgggtc
    18241 gatatattaa gccctgcggc aagggctttc gcgctcacaa aaaccatgcg tcgccaagaa
    18301 gattgaagac tcctcttctg cgaacagggc gaagaggctc aggccaattt agagaaattg
    18361 gctgggatga agctttatcg tatattgtgc aaagacttat tgatatcaga gccaaagacg
    18421 ggcctggttc tgtactctgc atagccagca gtggttcgac tggagccctg cacaatagcg
    18481 agactttggc tcgtcgattt ttcaactcca ttggcggatg cgtaaatgtt aaaggaaatt
    18541 attcatgtaa tgcggcaaca tatgcgcttt cgaggctttt gggcgcatca aggatgaata
    18601 gcggatttga tgcagagaca attagatatt ccaatcttat agttctgtgg ggctcaaatc
    18661 cattcgaggc aagacttggc gccgaacttc ctgcttattt gattgaagca aaaaagagag
    18721 gagcgcgcat catcgctatc gatccacggc ggagtatttc ggcaagaatt ctaaacgccg
    18781 aatggattgg cataattccg ggtacagatg ccgtcctggg ctatgctctg ctattcgaaa
    18841 tgctccaaga tgcgcgattc gatgcagaat ttatctctaa atattcgatt ggtttcgaat
    18901 cgttaacaga ttatgtgctt gggaagatcg atggaatccc aaagactcct ctatgggcaa
    18961 gctctatctg tggtgtcgat gctaacgtga tcattagact agcctccttc ttctgggatg
    19021 agaagccagc catgattatt ccgggttatt ccattcaaag ggtagcatat ggcgaagaag
    19081 cttttcggct gactatggct atacagcttg ctactcgcaa tagtggcctg cttggtgcat
    19141 cgacaggatc gataaacaac tgccttccag gaatcaaagt tgggactcta aaggaattgc
    19201 cttcctcttt gactaaggga gcttttatca aagagggctt tactaaggag gcccacctat
    19261 gggaggacaa ccataaatct caagggatag aagcggagtt gttcacaaag acagtgccta
    19321 tacttcgatg ggccgatgct gtgctaagtc catcttctta tgggcttggc tctattaagg
    19381 ctttatattc tgctgggggg aacttcctta atcaaggcgc aaatgtaaca aagaatatca
    19441 aggcctttga aagtgtagaa tttgccgtgt gtcatgaact atttcttacc ccttcagctc
    19501 ggtattgcga tattgttctg cctgtttcgg atggattcga aaaggaagat attggcattc
    19561 cttgggcagg caattatgtg ctctataagc cggaaatatg tagggcattt ccaggcacgc
    19621 attcggatta ttggatcttt tcacagttat ctgagcgttt tggtctggga tctgtcttta
    19681 cagaagggag aactgaatcc caatggatag aacaatttct tgctgaatcg gaaattgcag
    19741 atatagaagc ctttaggatc gaggggatct ttcttaagaa gggcctattg cgaggtgggc
    19801 tggacgattt tattgccgat cctgtgcatt gtccgatcgg aacgaacagt ggcaaaatag
    19861 aatttgcaag tcctttatgg aaccagaata tgaccttgca ttggcaaggt ctaatgaaga
    19921 aaggctcgac agaagaatct ttcagagaat tccggctgtt gacgccaaaa gtatcgaact
    19981 tcatccattc tcagcgcatt ggccaagaag accgatccga atgcgctcaa attcatatca
    20041 accccaagga tatgtcctat attggtgctc aagatggcga aatcctcaag gtttggaata
    20101 aaaacgggac gcttatggct tcagcactag ccgatgactg ggtccagccc ggtactgtat
    20161 ggatcgaaga aggcatctgg acaatgccta tcgatgaagt tgatctttgc ggaaacccca
    20221 atatgctcac ctcggatgaa ggaacacttg agagcatatc gaatatcatg catgggatac
    20281 ctatatgcat agggcggatt cctgaggcaa ccatcggcca ttgacttttg ttagtccata
    20341 tacattttct aatagccagg attgtccatt atgcatagtc attcctagct gttttaggct
    20401 tgggtcgata gaaggtttgt ctataccaaa gccacttgcg agaagcatct ctataagcat
    20461 ctttgcggca agaaagcgcc tatctttttg aactgcaaaa gcgataaaat ctggcaaaag
    20521 ggatactact tcccaggtaa cctgttctgc ttgtgaatag tttcctgcgt ctttgctctg
    20581 gtcagaaaag cgaataggga gggctctgga gatctcttct atatgtggga ctagccattc
    20641 gagatcaaaa atacctgtag cgcagttgaa aaggatggcg gaacccgatt tttctaggca
    20701 ttctacttcc tcaagtccta ttgcaggccc gatatctact acgtttcttg aaccgttggg
    20761 ggcaaggatt agtataccgc ccttaacatc cacaggtgtt ctgaatgcaa aatcaaaacc
    20821 cgcgggtttt cctgagatag ccaggatagc aagctctatt ggatctagat aagcccctgt
    20881 attgtccaca ttgctgagca ttgcatagcg cattccttgc tttagaagag ttcgatatat
    20941 gtcggcaagt atcatgaagc tctggccatg accacctgga atcgcaagag aagaattatg
    21001 ctttccataa gctcgatcaa aaacgcgctt tgggagcccc tcggccgaat gggtatatgc
    21061 agctattaga ggttgtattc cgcttaccca ttcacagggc tctagaccaa ctacgtgtgc
    21121 aaggtcgctt agatatggcg agactcgcat ttcttcataa gccctgagaa gttcttcatg
    21181 gttgcccaca cttgtcatct gaaaaagagg caagaaatca ggttgttttt gatatgcaga
    21241 ggatgcaagc gctccaggtt ttcttgacaa tctaccatga ccaagttctt ttgcaataag
    21301 aagacgagag cgcatcttta gctctagaaa acttgctcca gcactgccat cagggttgat
    21361 ataagcagga gtcaatcctt ttggtttgtt ttctatctct tttgccagat cttcaaaagt
    21421 cagccttact gcttcgaata gagactcatc tatagaacca ttctttttga tgtcgatata
    21481 acttgtagca gaaccaccat tcaaaacacc ataggcggtt tttggcaaaa gatgtttgcc
    21541 tatctcttcg agggcattct cggtaaagca aagcatttcc cttcccattt ctcttatttc
    21601 gaagatattt ttattaccag caggaaatgc ggaatggagg aatatgtcat tatgctctag
    21661 attcaaaatg gattcttgct tgggatattt gaaaagaata ttgttagggc ttctgttcct
    21721 tatatctatg acatggatac catccagagg cggcactccc gaaggtataa ccttttcata
    21781 gtgatcgaat tggccagcat tatagcgatt gagaatatcc agcgtgaggg taatgtcgat
    21841 gttctttttc ttcatgtcag cgagtagctc gatgtgcaaa tcctgttctt tcatctagag
    21901 tttccttagt gatgtgtgga gaattgaagc gctgctatgg cagcacccat cagagagatg
    21961 tcataattct tcacgatcaa aaccggagtt ctacagagta tatcgcgaat atgggtggca
    22021 taattcctct cgaaccatcg catgaatctt gacttttcga tgagccaatc ctcatttttg
    22081 gatgatatac cgcctgcaag ccagactcct ccagcaggca aaaagatgga cacgaggttg
    22141 gcagccttga gcgcatatag ccgaacgaac agttccattg tcaagctgca aaatgcatca
    22201 gacctggcat tttgtgaaat aagagccggc cactcaaatc gtggtaaatc aaggatattt
    22261 cgagcagtat cggagaggcc cttttccagc gctttttgcg aaatctcata gtcgccttcc
    22321 acgatactca ttgctagatc ttctgtgcat ataaattcaa atatatttgc aatgccctgg
    22381 cctgagatag ctagttcagc acctggcaca taatcatatt tcttgcgaag ccacgcgtaa
    22441 aaggaatcgc tcagcctatc gaaacaggac aattcggaat gtccaccttc ggaagggaaa
    22501 accatcacac tgtcgcctct cctatccaca aagccgacac caagacctgt tccagcccca
    22561 atgataagca taataccgtc ttggggctca tagatagatc catccggatg tggaagctgt
    22621 gtaaccttat ccttatcttt atgatcaaga atagtcacgg catatgataa ggccgtgaaa
    22681 tcatttatca atttgactgg cactctaagg aattcataaa gctccgaggc tttgataccc
    22741 caaggtgcat tggtgagttg aatgctatta ttggcctgaa caggaccggc ccccgaaata
    22801 cagcaaaagt cgattttatg gttagctccg cctccgcgcc ttgattcaag aaaacgcttg
    22861 atcggatcca agagggactt ttcattctga gttgaaaact gcgccttttc gatcaattcg
    22921 atgcccgact catcatgtct ggctaaggct agattagtgt ttgtccctcc aacatcgcca
    22981 acgaggaata atttgttcat tgctatacta tagcgaagaa attattttgg tgaaacagct
    23041 gtcttgttct ttgaagagtc tttcaaaagc gcgataattt gtgtatttct tagaaaacgt
    23101 ccgctctttg aatcgaagat ttcgagatga agatgatctc cccctccttt tttgcgtgca
    23161 ttgatgcccg aatttccccc atgacctatg gcttggcctt tcaagagaat atcccctttt
    23221 tttacaaaga gatcatagag atggaaatat aaataatacc tatgttcttc aggtgaatat
    23281 actattacgc cattacccga ttttggagat attcctcctt ttatatagga aagagcagca
    23341 ctctttctag gaaacccaag ccatccgctt tcagcagcca ctacgatacc attgctcaat
    23401 gaatggataa cagggccacg ctgaatattc caggagagat actccacatc ttgcagaaaa
    23461 atatccagcg cgaatgtatg cgaatagtcg agctcatcaa gccgaggcag tgaggaaagg
    23521 ccaggaaggg tcacattgcc attccatgtt tgtgcatttt gctcgaacca ttttgcaggg
    23581 aagtcagata taccgctttc taataatctt cgataaaaaa catcgataaa tgcttcatat
    23641 cgaggtcgaa gggcattcat ctctggatgc aatgctgcaa tacgatcata tgagagtctt
    23701 gcaggcaagg atattccatc tagattttga tattcttcgg agtcttgatc gtcgatgagc
    23761 atggcaagaa catgaccaga agatggaagg aacaaagaag ccattgccct attgtctaaa
    23821 tccatagtca tgccttgaat gcaaagacta gacgctagtt gttcaatttg tttcatcgaa
    23881 agcaatgaga aattactgct attcaacata gagaggttat gatagaaatc gttctccata
    23941 aaagatcgct cattattatt gggataagca gcaatttctg aaagcgatat aggcctcgtg
    24001 tcagaataag gattaagcga aagcatcgcg caacgaaaga ccaataagca aacaattgat
    24061 ataaccgaaa tcgttacatg tttgattatt cgaaaatcca cgggcttgcc tttaaggtca
    24121 atctctcaag atcatcattg cttttagagg gtcgtcgaat tattgatcat ttgcgatgcg
    24181 aatcgaccat tgcagtaatt gcttctgcta taagagctat tttcttttgt gtgatttctt
    24241 tgcttaagct aagttcgctg ctctgatcgt ggcagagaat gtagtacttt atcttgggtt
    24301 cagttccaga tggccttaca gtgagtagcg tgccgtcttc taggcgccat tggatcacat
    24361 cgctttgagg cagatcgatt ttgcgaattc tgccatctct atcccactcg gtaccggctt
    24421 tgacatctcg agtgcagatt actgcaagct ctccaaattg atctggctgt tgctttctat
    24481 agtcttccat gatgctattc attatcctca tgccttgttc accttcaaaa tagaagctta
    24541 gtcctttttc ctcgtaataa ccgtgttcct tgtatagatc ctcaaggcgg tccaggaggc
    24601 ttttgccctg tgatctccaa tagagggtca tttcagcagt aagggccgct gcggaaatac
    24661 catccttgtc tcgcacttcc ttttcgatga gatgaccata ggattcttcc gttgcataga
    24721 tgaaatcgat attctcggtc tcgaagcgtc gcattagatc tgcaatccat ttgaatccag
    24781 taaggcactc aaagctctct atgccatatt tctcagctat agccttttgt aattcggtgg
    24841 taacgatggt gcggatagcc gccggtctag gtggcatgct attgatttcc ttaagagtaa
    24901 gcgcaatgta atcgagatgc agtgacccga gctgatttcc tgttaccaat acgaattctc
    24961 ctgctttgtc cggaacggca atgccaagcc tatctgcatc tgggtcggta gccattacca
    25021 catcggcatg agttttcttg cctaactcaa ttgccatagc aagcgctgct gattcttcag
    25081 gatttgggta agacacggtt gggaattcgc catccggttc gcgttgctct ggcacggtaa
    25141 ggacattcaa acccagctct ttcattatac gctctaatag tcttgcccca gtgccgtgaa
    25201 gaggagagta aacgatattg accgatttac ccatttcgga gaaaagatat gaacgcaaaa
    25261 gatgcgattt caccatggca acataatcat cgtctatctc ttggctaatc tctaccaaga
    25321 gcccttttga gcgagcctct gttttagaca tctgctttgc agactttgct tttaatacct
    25381 tttcgataat accactatca tggggaggca cgacctgaga accatcattc caataggctt
    25441 tatagccgtt ataattggga ggattatggc tggctgttat tactacacct gtgtctgccc
    25501 caagctttcg gatagcataa gatagaacgg gagtaggctt gagctccggg aatatatatg
    25561 ccttgatatc attggctgcg aaaaccaagg ccgttgccaa cgaaaactct ttggattttc
    25621 tacgagaatc ataggcaatg caagcagaaa gctgcttctg agggaattgt tccttaatat
    25681 agtcgcatag tccctgcgtt gctcgtgtta ccactagtgt attcattcta ttgaagccac
    25741 cgccaataat gccgcgaagc cctcctgtac caaattccaa atctctatag aagcggtctt
    25801 caagttcctt ccaatcgcct ttctgaagga gctcttttat ctcctcagaa aaagacggat
    25861 cggtttcatt agcaatgtat tcctgcgctc tcagtattat ttcctcatga ttcataatga
    25921 ctcctcgata agaccaaatc ctgcaatgca taaagagcaa ttccaagcct tcttatttta
    25981 tttctaatta tagctattgt gctctaataa gaaaagtcag cctcccaaaa gagagatcga
    26041 tctttgggct ctcagttatt tgaactcgac aatggagagt agttctggtg gcgaggttat
    26101 aagatagtct gctccggcaa gctcgagttc ttctctgctt cgataaccat atacggcacc
    26161 cacagctgct attcgtgctg cttttgctgt ctgtatatcg acagccgagt cgcccacaaa
    26221 caggcttttc tgagggtcaa cccttgcatt gcgacagagt tcaagtgtag cgcttggatc
    26281 tggtttacga gctcggtctt tattgtctcc ccagacagcg acaaatttga cagaggcaaa
    26341 aagagattcg atgatctttt ttgagagatg gtctggcttg ttcgagagta cagcgagttt
    26401 tatctcattc ctgtagaggg attcaagagt ctctaccgtc ccagggaaag gccttgtcct
    26461 atcaagcgca tgttcttcat atctctgcat agaaagtctc agtatcttgt cgaagaatat
    26521 atcatcattg gatttttctg gcgaaaggac tctttgaaca agaacacgaa agccattgcc
    26581 taccatttgt ttgtaatctg aaatctcgtg attaggaagg ccaaactgag ataacacata
    26641 atttacggat gaggcaatat cgacaatggt atcgacaagt gtcccatcga gatcgaatat
    26701 gataagttca ggctgcaatt ttggcactat gaggactcca agttctatag attatggttt
    26761 ttcgcttatt cttttgattt tatattgagg tcgctatctt taccgccttt ggatctatat
    26821 agattttcct tctcatcgat gagccgatct aagttctgga gctctcttat tataggctca
    26881 atttcgggcg tcgaagagcc tatcatgggt tcatttttct ctaccagaag gttatagact
    26941 tctgtgccga gcttcgcggt gagggattgt gcctttgtcc ttaactgtat aatttcgatt
    27001 ttcagcctac ccatctctcc ccagacttgg gcttgatccg cggctttttc caagaattct
    27061 ctagatgctt cggcgctgtt ttccatgaat tttcttagct tttcggaaaa gtccattttg
    27121 acctcctttt ttacagtatg ataggcaatt catcatcgat acaataatcc cagagaaaaa
    27181 aattagcaat ataatggaac gtacgaacag aaaagaactg ggcatgtcga tagcgcagta
    27241 gattcatttg gccagagaat tgttcagctt ttgcgaaata gggctaaata cactatactt
    27301 aattgaaata gggggtatat gaggtgttgt ggatttatac gatttttgcg gtcctttccg
    27361 catggtgggt agtttttatt ataatcgctg caattcgaaa acaaaaacct gtctttctat
    27421 ttcttctaat cctatttttg gtatggggga tttcggagat tatattgatg atgagttctc
    27481 ctccgatagc ggctattttg ttcattgtgc ctttcttaat tctgattcag atgatgcgcg
    27541 tcgttcagga ataccagcga ggggttttat ttcgatttgg tcgcttacaa aaagtgatgg
    27601 aaccaggctt caacctcatt ctaccatttg gtatcgattc acttcgcaag gttgatatgc
    27661 gcacgttcac catagacgtt ccaaagcagg aagttataac ccgagacaat gtgcctgtca
    27721 ttgtggatgc tgttgtgtat ttcaatgtgt tcgattccat gcttgccgtt gtaaaaatag
    27781 cagattatac caagggcacc agtctccttg cccagacgat tctccgatcg gttcttggtc
    27841 agcatgagct cgatgaaatg ctcactcaga gagctcaact gggacaagtt cttcaaggac
    27901 tcttggatga tgctactaat ccttggggta ttagagtctc ggcagtcgag ataaaagcga
    27961 tcgagcttgc agagactatg aaacgagcca tggctaggca agcggaagca gagcgagaga
    28021 gaagagcaaa gatcatcgcg gcagaaggag agcttcaggc ttccgaaaag ctcgcccaag
    28081 ctgccgatgt gttagcgaaa tctccggcaa gcttgcagct gcgatacctt cagactctat
    28141 cggaaattgc ggttgaaaaa aactcaacta tagtatttcc tctgcccatg gatctattga
    28201 atgttatctc caaggtcgcc gaaaagagtt cagaatgaag tgcccatatt gcggaagccc
    28261 cgaagacaag gtgatcgact ctagaacact ggctaacgga gaagccataa ggaggcgcag
    28321 agaatgcatt tcctgtgggc ttcgttttac cagttatgaa cgcatagaag aaaagcctct
    28381 ccttgttatt aaaagagatg gcaggcgtga gcccttcgaa agacagaaga tcgaacgagg
    28441 tcttgtccgt gccctggaaa aaaggcctat ctcacagatg tccatagaga atctcgtcaa
    28501 tgaaatagaa gacaatgctg ccgaacatgc aaaagcttct aatgaaatat cgagcgaaga
    28561 attgggtcgg atggttttgc aaaagctata ctccctggac aaagttgcct atatccgttt
    28621 tgcctcggtg tatagaaaat atgacaccct cgatgaattc attcgcgaga tcgaacgcct
    28681 ggaaaacggg cagaccggct aacggagatc gtattattgc tagttatcac tcttcttcag
    28741 cttcctgaat cttgaaggct tgcgctgcct ttatgacatc ttctgcaatc tttccagaga
    28801 taggcgcata atgcgagaat tctacttcga aggaaccagt accggaggtc atggaacgca
    28861 gatcgatcgc atagcgcaaa agctctgcct gtggaacttg tgcatctatc tgaacaatgc
    28921 caccgcctat ggggttctgg ccggatattt tcccgcggcg ccccgaaaga tcggacatga
    28981 catcgccaag gtatttatcc tctacaaaga cagagagatt catgattggc tcaagtaaga
    29041 ccgggcttgc tagcttcatg gcttcacgaa aggctcctct agcggctagt ttgaatgcca
    29101 tttcagatga atcgactgtg tgctcttttc catcgatgat cgctgtttta gtatcgacta
    29161 caggatagcc agccaacacg ccggcttcca ttgcttgatg aatgcccttt tctatgcccg
    29221 gcatgaaacc tttagatacc gcgccaccaa aaattctatt ctcgaattca tagtcttttc
    29281 cgcgttccag tggttcgatt tcgaatacaa ctcgggcata ttggccatgc cctcctgtct
    29341 gtttcttgtg agtatattcc gcagttgcct tcttggttat ggtctctcta taagccacgc
    29401 gaggaatcct ggtttctgcc gctatctttg attgagattt gattttgtcc agtatcatgt
    29461 tgatctgctg ttcgcccatc cctgcgatta ctgtttctct tgtctccgga ttatagctca
    29521 ctctaaaagt gaggtcctct tccgcggctc tatttagcaa ttcgttgagt ttatcttctt
    29581 cctttttatt cactgcggaa attgccaaca tatggacagg agagggcaac tgcagcggtt
    29641 cataggagat tggcttatcg aattggctga ttgtatcatt tgtcttgagt gaggaaatct
    29701 tagcaaggac tccgatatct cctgctggta aagaaggtat ttcttcgagc ttttttcctt
    29761 gcatagtata gagttttcct atgcgctctc tgtgtaaatc acggctgact accatttcta
    29821 tatcggaaag gatggttcca gttatagctt taatataaga aagtcgacca gagaattgat
    29881 cgatttgagt tttgaatacg taggccgatg caggcttcaa aggatcgata gatatttcca
    29941 catcattgcc atcttgatcc ttagcctttt ccggagcacg agcaaatggg gaaggagcac
    30001 tctgaaaaat aaaatccata agcgcggcgg tcccgctatt tacaaaccct gctccggcaa
    30061 aggctggaat aatcttgcct tcggcaagag cttccttaag accaagcaga gtctcttctt
    30121 gagtaagctc gccttctaga aggtattttt ccataagatc atcgtttcct tcggcagctg
    30181 cctcagagag aagtgcgcgg gcggattcca cagcttcgga atactcaggc ggaacttcac
    30241 tgggtacttc cttttgatca tgagagacag gtcgcatata tgccttgtta ttgagtacat
    30301 cgattattcc cttgaaagag aacccttctc ccataggaat tgtcaccgga acaggagtga
    30361 ctcggaattt ttctttgata tcagcaagag cattcgagaa gtcggctcta tcttcatcta
    30421 gccgcgtgat aaacatcatt cttggtctct tgtggcgctc cagaattctc cagagtttta
    30481 ccgtctcaat ttgtacaccg gatttgccat cgataacgag cagagcagcc tcggtcgaac
    30541 gaatagctag aattgcttct ccaacaaaat caccggagcc tggtgcatct atgaagttga
    30601 tcttgtatcc atcggtgtta acatgcgtaa ggctggagcg aactgatatc ttgcgtgcaa
    30661 tctcgtcttc gctataatcg ctgacggttt tgcccgaatc taccgtttca ggtctagata
    30721 tcattccgcc ctgaaagaga atgtgctcaa gcagagtcgt ttttcccgtg ctaccatgtc
    30781 caatgatagc aatattacga atctgttccg tggtatagga cataatgact cctcacaggg
    30841 tgggatggtt tagaatttcc tctcataatt taaggaagac gagctattat aattcaaaag
    30901 gcgtcggtgt caacagcagt ctgacaaata tagttgggat tcatctttgt ccggtgcttg
    30961 caggcatgag cgcaatgcag tcgaatacag tccgtcctcc ctgataagca gtagcacttc
    31021 gcctgataat tctccaaagc ccattctgat atttgccctc agtttcaatc ctttcggtca
    31081 agccagcggt tgtctctatg aaattcatta gttcgaggtt ttgtgttaga gcaagtggct
    31141 ttaagcccat acctgcaatt atcgagacag gttcgattat cagattggac agctctgcta
    31201 caggttgagt tgccatggct gcctttgctt gtagacgaat catgcgcgct cttgcatctg
    31261 gtccaaccgt atccttgctc actgatatta gatccagcaa ataattgcga acatcattca
    31321 attctcttat tgttgataat ttgtctattt ccggcgaaga cttgagcacc ttggccaaag
    31381 ttgatatctc tcgaagatag tggtttcgct gattagaagg aaccagatac atgactatta
    31441 tcgagacagg tctaccatcc ggagctccat aatcgatacc tgtcggactc catcccacta
    31501 cgcacatcaa atcttcttcg aattccacgc gcgcatgagg acaagcccac cttctgccaa
    31561 gagccgtatt catagatttt tctcttgcca taacattacc cacaatatct gttccggtgg
    31621 gaatttgagg gaaggcttcg attatatgcg ctagaaactg gagagcatga tccttatcat
    31681 tttcaggaag ctcaaaaagt ctgccttcct ggagggcatc tagtatcgag tccatggtta
    31741 tgctccttca taatgtttta aaaaccagca ttttactttg tgcgtgataa tagaataggt
    31801 caaaagaaat gccgctatcc aaagccaata gatataagga agaggcacaa ggccaagaat
    31861 acctgctatg ggagagtagg gtagccagat tccgattccc atgacaagca atgatgcaag
    31921 aatcattgga gccgatgccc tgctctgaag gaaaggaata cgtttagtgc gaattatgtg
    31981 aacgatgaga gtctgggtta gtagtgactc gacaaaccaa ccggtctgaa aaagactggc
    32041 tgcagcatcc cgttgtgcta aagaaagggc agggttaagg taatcttttg cattgaagaa
    32101 aaaccacata agtgcgaaag tagcataatc gaaaattgag ctaattggtc caatacacat
    32161 cataaatcgc tttatattgt cgatatccc
//
LOCUS       DAOMZL010000010        31852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_1073_length_31852_cov_15.014561, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000010 DAOMZL010000000
VERSION     DAOMZL010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 31852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31852
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1073_length_31852_cov_15.014561"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..156
                     /locus_tag="PLQ30_01735"
     CDS             1..156
                     /locus_tag="PLQ30_01735"
                     /inference="COORDINATES: protein motif:HMM:NF039902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01194.1"
                     /translation="MGKKLGDILMPLRVAITYSRVSPPLFESMRILGKEECIVRIEAA
                     IAYLRGA"
     gene            225..1952
                     /locus_tag="PLQ30_01740"
     CDS             225..1952
                     /locus_tag="PLQ30_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPW01195.1"
                     /translation="MSESIEKPEYLDFIREAVAEDLRIGRCKEVRTRFPPEPNGWLHI
                     GHAKALFVDFGVAQDFGGKCNLRMDDTNPEKEDMEYVEAIKRDIKWLGFDWEDRLFFA
                     SDYYERLFELACKLINKGLAYVDDLDEEQIKEYRGTNVPDKNNITFTPPGRNSPWRDR
                     SPKENLDLFERMRAGEFPDGSKTLRAKIDMAHPNLLMRDPVMYRIRRIPHYRTGTRWC
                     IYPMYDFAHPLSDAIEGITHSLCSLEYEIHRPLYEWFIQNCEVFPSRQIEFARLNLSH
                     TVLSKRWLLQLVRGKKVKGWDDPRMPTIAGMRRRGYPAAGIRDFLGRIGIAKTDSLVD
                     IQLLEHCIREELNAHANRYMAVMNPVKLVIENWPKGKIEWLEAINNPEDPSAGIRKIP
                     FSGTLYIDREDFKETPPPKYYRLYPGNQVRLRYGYVVTCTGFEKDPISGEIERIRCVY
                     DPNTRGGDAPDNRKVKGTIQWVCAMHAVPIEARLYDHLFDAERPMEVPEGKTFFDNLS
                     DHSLIIESRAVAEPAIAEIGKGETVQFERIGYFCKDLESTSDLAIFNRTVTLKDTWAK
                     IEKKLQRNP"
     gene            2010..3386
                     /locus_tag="PLQ30_01745"
     CDS             2010..3386
                     /locus_tag="PLQ30_01745"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPW01196.1"
                     /translation="MSETVVTMEKIVSLAKRRGFVFQSSEIYGGLSGAWDYGPQGIEL
                     KNRIARFWWKEMTQMHDNIVGLDAAILMHPRVWEASGHVENFTDPLVDCKKCKSRFRA
                     DQIPPENLAAKKCPDCGGELTDTRKFNLMFKTTLGPVEDESGLVYLRPETAQGIYVNF
                     KNVQQSNRMKIPFGIAQIGKAFRNEIVTKNFIFRTCEFEQMEMQFFVKPGTDEDWFEY
                     WRSERWSYYQKMGVNIDKLRWHKHGPDELAHYAKDAYDIEYEFPMGFKELEGVHNRTN
                     YDLSRHQQYSGKDLEYIDQDSGNERYIPYIIETSAGLTRQLLMLLCDSYEEQKISDKG
                     NEDDWRTVLHFHPVLAPTTVAVLPLMKKDGLAELAQDIRIELKDDFVTDYDQSGAIGR
                     RYRRQDEIGTPFCVTIDYQTKNDNTVTVRDRDSMLQERISRANLTSYIQHKIKNYHAP
                     NRSEVACS"
     gene            complement(3536..3835)
                     /locus_tag="PLQ30_01750"
     CDS             complement(3536..3835)
                     /locus_tag="PLQ30_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPW01197.1"
                     /translation="MPSTGKPLTKVEIIDSLAKSTGFTKKDVNLLLDEFAKLAYSEAK
                     KNKKFTIPGIAIMKVVHRKAKVGRNPATGEQITIPAREVIKITVAKPCKDAVLGE"
     gene            4081..5310
                     /gene="tyrS"
                     /locus_tag="PLQ30_01755"
     CDS             4081..5310
                     /gene="tyrS"
                     /locus_tag="PLQ30_01755"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPW01198.1"
                     /translation="MNRALEILKERGFVQQCSDLEGLSKLMDERPITFYIGTDPTGPS
                     LHIGHMVPFFAMRHLIGAGHRGIALLGGGTARIGDPSGKAEMRKLISYEEIDANTERF
                     KAQLDNFIHFDGDHALTANNKDWLSNLNYIEFLRDIGRHFSVNRMLSFESYRMRLETG
                     LSFIEFNYQLLQSYDYLQLHKLHDCRLQIGGDDQWGNIVAGIELIRRVEGAECFGLTF
                     PLVTTSDGRKMGKTERGALFLDPDITSPYEFFQYFRNVPDGDVERFLLMFTFLPIEEC
                     RYLGSLKDKALNEAKERLAWEVTSLIHGQEEADKALSAAGAAFKGEGDASQLPTIEVE
                     ISRLDSGIGVLDLFVEAGLAPSKSEARRLVKQSGASVNGRKIENEMQTLTSSDLIDGA
                     LMLKAGKKRVARIVPKL"
     gene            5318..6043
                     /locus_tag="PLQ30_01760"
     CDS             5318..6043
                     /locus_tag="PLQ30_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPW01199.1"
                     /translation="MQHLFLALADIHGNINALEQILKANSNIEGIFVAGDLTNFGKEK
                     EAQRVLLAFSSIAPKAKLYFLAGNCDTAEARKCFDMQPGYIEQRCCALLPEKEIWIIG
                     CGGGLYHTGLTPFEIREEGLESGIKQALKCAIRGYRDISSMDGSEPTPGLIVLTHTPP
                     KDTFSDMRHSRHIGSAAFQTFLYEQEPLLWICGHIHEGKSIHIENKTLVVNPGPAAHG
                     LYSLILLKKSPKGWKAAAELRSI"
     gene            complement(6059..7042)
                     /gene="hflC"
                     /locus_tag="PLQ30_01765"
     CDS             complement(6059..7042)
                     /gene="hflC"
                     /locus_tag="PLQ30_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HPW01200.1"
                     /translation="MKKASIIITVVVALAILFIVIFGPLFVLNEGEQAVVVRFGKIVN
                     SHSDAGLKWRSPIIDTVIKYPKRLMSWDGEPQRIPTKENQFIYVDTTARWRIADPIKF
                     YESVNDLNAAYSKLSDVIDSAVRTVISSNYLREAVRSSDEILSENQVESFQTGDVENS
                     ATLQQLAITPTQYEKIEKGRLELTEEMRALVSDIVPSFGIEVVDILPRQIKYSDELSE
                     SVYQRMIKERNQIAQAFRAYGEGKKAEWLGKLENEKRTILSSAYAKGESIKGKADAEA
                     SKIYADSFGRDPSFFEFWKAIESYRKTMPAFNKTLSTGMDYFKYLYSPTGR"
     gene            complement(7039..8028)
                     /gene="hflK"
                     /locus_tag="PLQ30_01770"
     CDS             complement(7039..8028)
                     /gene="hflK"
                     /locus_tag="PLQ30_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HPW01201.1"
                     /translation="MADIINPKKKLNVKLKWPIVIIIIVALAVLVFLLTSFVVVDQTE
                     KAVITTFGKYTKTLGAGLHYKLPFGIQKAHLVKTQVVQTEAFGFRTVKPGIITQYSNE
                     KFPDESTMLTGDLNIVDVEWIIQYRITDPKAWLFNVMDKEKTIRDTSQMVLNMLVGDR
                     TILGVIGPERQSIQETAVTLMNEFFSIYGLGITVTQVQLQNIVPPEGVQAAFEDVNKA
                     IQDMNRLINEGREAYNAEIPKVQGQADQVLEVAQGYASERVNVAKGDVSRFNSVYEEY
                     RKAPEVTRKRLYYEMMEEIFKDQEHVDLIDKSLQNLLPIKNLAQTPSSQEASR"
     gene            complement(8006..9070)
                     /locus_tag="PLQ30_01775"
     CDS             complement(8006..9070)
                     /locus_tag="PLQ30_01775"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPW01202.1"
                     /translation="MGKLLIITLSRDDDGRRADRIVRKVLKDTALSTIYRLFRQGDIR
                     ISNMPIAPNDRLKEGDVIEIKFSERSLENGQNQTIIKSTLPKKEYSQVAFSEIPRILW
                     QNAQLIAFDKPRGILVHDGSSSLNAYVQRILYDQLEPSASFSPGPLHRLDRNTSGIIF
                     FSKTRKGAELFSVAMKNRRIRKFYAAIVQGKIDEPISLKDFILRDKKRKLSLLDDRKG
                     KEASLYLSPLMHNKSYSFLLIELITGLTHQIRAQLASHGYPLVGDSKYGGSKTAKISS
                     YFLHSCCLGFPEPIFEDMPLTIFDPLPGDFNLAVSDLFQIDHEEVDALISHRIEQSLE
                     ICDNNLFPEKGAKHGRYYQS"
     gene            9218..11134
                     /locus_tag="PLQ30_01780"
     CDS             9218..11134
                     /locus_tag="PLQ30_01780"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1925 domain-containing protein"
                     /protein_id="HPW01203.1"
                     /translation="MQNFKVVIGMYNNLPYNASGALYEQTYQGAWRPFLSGLYKFLSI
                     KSAIYFSGAIFPWLEENHPEYMYLLVEMVRRGQVELLGGGLYNPLSPLISAQDMTGQV
                     ESLSAFIRKTTGKRPSGAWLYEYSWSSSLPSILQNSKIQYTFLPAEYFPNVEGGMEFG
                     LPVTSEDHRKVITIFPTFESKDSENSFMPYENTLTDLQESYPECKTFIIMADGNEISM
                     SWEKSGLESPDVLFERTFAWFQKNCLEYDTLTPIQLHKTTKPSRIVYFSQCYSRRFKE
                     YCKKTINRLPIQNQEYIQISKQSVLDHPLVHVLYQKLNYVSTMIALFRGDKSRKKASI
                     DDIWKAQCGDLFWIGPCGGILIPEARIAAFSSLIEAEKTIRQSRFRPYLSFDDLNFDG
                     LKEAIFQSSVYNCYLQSGSASVSEFDSIKTGTNYACGWNDDQFSTGCFKDYIKTEGNF
                     ERDLIAVERWNMVENPREESSVLFRREFSARFEDRLLMLLCRKTYRFRNDFFSIDYEL
                     SNKNTEACLFRFCTNSEIIAAPIFEDHRFELLHHRESKALDLRSQAFFETVDGIGLSN
                     VRKAERVLIRSDLPFSLLAKPNFLKKPSVSPQVMDVPSDSLMFEGFSIGIGWDLSIPA
                     EGTAFFSLSVHLEH"
     gene            11213..12748
                     /gene="murC"
                     /locus_tag="PLQ30_01785"
     CDS             11213..12748
                     /gene="murC"
                     /locus_tag="PLQ30_01785"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPW01204.1"
                     /translation="MEMIQFPDKLEGLHIHLVGAKGTGMTALAEILQKRGAILTGSDV
                     ADVFYTDRILHSLGIELFENFDKKNLPISTDIVIHSAAYSREGNPELKAATHRGIPIF
                     SYPEVLGALSLHSSSAGIAGVHGKTTTAALAGILIKALGLPATVLAGSAISNFDDRCT
                     LVQGESFFIAETCEYRKHFLNFKPTWIVLTSVESDHQDYFPTYESIRDAFVEYVLSLP
                     PNGVLIFCADQKGAVEVADIVLQRRRDIKYVEYGFLAHGKYGIRDFQTKAGASSFILG
                     ESSFPIRIHLPGRHLVQDSVAAIALVEQIFETVKSRPFEESEWISVGEALAAFRGSKR
                     RSEIIGEYKGILIMDDYGHHPTAIKTTIEGIKKFWPHRRLIVDFMSHTYTRTIALQDD
                     FISSLDGADSVLLHKIYASARERPVSGFNGRTLYQKLCEHRPELFPMDLERDTRQKEI
                     AYYKNKASQDQTGFVAYSDEPLKALDALSNLLQPGDVFLTLGAGDNWKLGKALAEKIE
                     KNG"
     gene            12795..13661
                     /locus_tag="PLQ30_01790"
     CDS             12795..13661
                     /locus_tag="PLQ30_01790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPW01205.1"
                     /translation="MKSMTGFAHSPILEKEFQGTLTLKSFNNRFLDISIFLPSYASRI
                     EPLIRDFLGSRILRGKVECTFKLRKIKFSLEDIELQFQAATLLSEQLRKLAEACGIEK
                     DLSLDLITKFPGIVDMNTEIEEDYLWPVLMPILEETWLKFEESRIREGTATEENIRGQ
                     LQRLKSKLIEIELQADSLERMVYDQLVARFKELLSESYDEGRVLQEVAVQLVRLTINE
                     EIGRLRAHIDAFELMAKEPACGKKLDFLCQEMNREINTIGSKSMLVSVSHAVVEMKDA
                     LENIREQLRNVE"
     gene            13651..14214
                     /locus_tag="PLQ30_01795"
     CDS             13651..14214
                     /locus_tag="PLQ30_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW01206.1"
                     /translation="MWSRIGMGIIAISGKSGCGNSTVLRLVAEKLGYEPVNYTFRSMA
                     ADMGISFQELHKKAQENLEFDRRLDEHQVLLARKGNTVIGSRLAIWMVPEADLKVYLK
                     ASHAVRVRRIHAREDGDIEAIDQFTKERDAKDRGRYLALYNIDIDDVSNAHLVIDTER
                     WSADQIADIIVDAYHLIPLGDPYGSSL"
     gene            14198..15019
                     /locus_tag="PLQ30_01800"
     CDS             14198..15019
                     /locus_tag="PLQ30_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW01207.1"
                     /translation="MEAASENKRVAFVTGATSGLGLAISRSLIDIGFIVYGAGRRKPP
                     LDLPDNYVYVQTDVALDESVNKSRDFVLSQQSHIDLLVCAAGFGISGSIEDTPIDSAI
                     DQFNVNFFGVARVVCAFLPGMREQGRGRIIIIGSIAGKTGMPFQAYYSASKFALEGFV
                     ESLRYEVRRFNIEACIVEPGDFKTGFTGSRQKIIAPESPYYNQFERVISVQEHDEIHG
                     LDPAKLGERIARLAVSKRLPARLTIGPLFERLAVWIRRILPDAWFEAFYRMYYHL"
     gene            15099..16151
                     /locus_tag="PLQ30_01805"
     CDS             15099..16151
                     /locus_tag="PLQ30_01805"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HPW01208.1"
                     /translation="MHVATSSIEKKHFGTLSSGEEVSLFILKAGSFQASFTDWGATWV
                     SLIMPDKEGQKDDILLGFSTLSGYVGKHPYFGSTIGRFANRIGGARFVLNGIGYKLWT
                     NDNCNHLHGGRKGFDKFLWNYELSAFDGEPAIRFSRTSPDKEEGYPGTLYAEVIASLS
                     SLGELHLHYYAETDAMTPVNLTNHAYFNLAGEGKGTILDHELEMQAGMYLPVDEYLIP
                     NGEICSVEGTALDFRCPKRIGRDIQEVGNGYDFCYIIDKDRLPLKTFAIVTEPSGGRS
                     LSISTTLPAVQFYTGNNLAGTFGKSGSIYDKYAGFCLETEMYPDSPNKQNFPSCILLP
                     GMPWEHETVYKFSLQC"
     gene            16166..17297
                     /gene="prfB"
                     /locus_tag="PLQ30_01810"
     CDS             join(16166..16234,16236..17297)
                     /gene="prfB"
                     /locus_tag="PLQ30_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702248.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPW01209.1"
                     /translation="MLEDISKPIEDIKEDILEIWGRLDAPAIEDKIHDLEGLASAPDF
                     WSEQTQAKKILSEIKILRDRLETWQTMHNDILTLEEMYELARQEDQQDMEADLRVSLE
                     EIRTRFDRAVVIEMLSGEADRNDAYLTIHAGSGGTEACDWASMLYRMYVRWAERHDFK
                     VETLDFLEAEGGIKSVTIQISGEFVYGYLKGETGVHRLVRISPFDANARRHTSFASVS
                     VMPVLDESIEIDIRPEDIRIDTYRAGGAGGQKVNKTDSAVRITHLATGIVVTCQNERS
                     QYKNKDVAMNILKSRLYEYYKAERDKETAKFAGEKKEIAWGSQIRSYVFQPYTMVKDH
                     RNKFSVGNVQAVMDGDLDPFLEAYLHWQWKGGTSVEEEDEDI"
     gene            17284..18204
                     /locus_tag="PLQ30_01815"
     CDS             17284..18204
                     /locus_tag="PLQ30_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01210.1"
                     /translation="MKTYKDLFLFPLFLFLLIDTSLFAQITPSQNKGLGSIALVFPIQ
                     VSNLPDFDPLGQENLDSIMILEFIQDRLSDPQYYENKPSPSEANSLSRLMEETLRLRT
                     TSEELIAIDIETLKNWNWNRIIQEKSALFQEGSLKKSDAVLFMMYTIEDSNMQLASFL
                     LKNTGESYAFLSNRFSVFDLLDAARKTVDSFIEKGALLAKKSTPVQSELSIEEIELPS
                     IDPWKNRDNYKKAQSAFETSMGGAAISLSISFVAVGLWNIYREAAYRNSAFDVAATIS
                     AIAAGTSAAVTATFLTISIWNAGKMLGASH"
     gene            18230..19096
                     /gene="ftsY"
                     /locus_tag="PLQ30_01820"
     CDS             18230..19096
                     /gene="ftsY"
                     /locus_tag="PLQ30_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPW01211.1"
                     /translation="MNFKDKIKALFHRSQLSEEVFEELEDLLIEGDIGASIAFEVVDA
                     LKDTCRKDNISTPEEARKGLKKILRPFIRVEDFNLDDKRLNIVLILGVNGVGKTTSCA
                     KLAKWAENKGINQPIILAACDTFRAAAIEQLRIHGQRLGARVIAQQHGSDPGAVLWDA
                     IDAAKKNNANLVIADTAGRMHTRADLLRELEKLDRVVSQKAGDVNYRRLLVLDATTGQ
                     NAMRQAEIFNGSVKIDGLIMAKYDSASKGGMIIAVSKQFGLPTVFLGTGEKYNDFEPF
                     DIDKYLDDFVGD"
     gene            19111..20442
                     /locus_tag="PLQ30_01825"
     CDS             19111..20442
                     /locus_tag="PLQ30_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW01212.1"
                     /translation="MIKTSDEKKQRWMLSGMRFRLPLYIAIRWSITGKKSAHSSGLKF
                     ASLGIAVGVIALIVVLSVMNGLQLQYIESLLETTSFHIRARIEESNLDIFTDTLRKNR
                     LVKSVTPFIETNLLVQSSEDRQNVLRVMWIDPEDLALDSGYCKALNILPSAAAKSLEE
                     GIILGSEAAQILGVSEGSILTLRGAFVDPEEGIQGYSERVKVSSIFRSGYYELDAAFA
                     IMPRLKAKDLAGFPASTILGIKLINAEKSISFVSMLEQEPGIIDIESWQNYNRSFFSA
                     LHTEKILMFLLVGIIFLVVAINIHYSMRRSIARKSRDLAILAAMGMNQHSISSIFMLE
                     GILVGLAGALFGIAIGIPIAQHVDGIINGAIGLIESFISFLYRLGFVKGVPDLRIFSP
                     SIFYIEGIPSRIFISDIAVIAAFALIFPVIAVYLAYRRFRNVSPLEVLRSE"
     gene            20435..21118
                     /locus_tag="PLQ30_01830"
     CDS             20435..21118
                     /locus_tag="PLQ30_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW01213.1"
                     /translation="MSNPIIECRDLTKTFSSSAEVLCILDRANFAMKRGQICTILGPS
                     GSGKSTFLAILGSLERFDSGDVHVDGYDLSSLSEKSLSMFRRNIVGFVFQFHFLLNDF
                     TALENVALPSYVAGTPKNEAWDRARRLLDMVGLGKRLDHFPSQLSGGERQRVAVARAL
                     INSPSIVLADEPTGNLDASSAKELRSLLQNLPESAGTTLLIVTHDPDLAAIGGSSYLL
                     KGGRFECIK"
     gene            21106..22428
                     /locus_tag="PLQ30_01835"
     CDS             21106..22428
                     /locus_tag="PLQ30_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPW01214.1"
                     /translation="MHKIRLSGLKPFSYLGFRMLLGRPAAAREKSAHSARHGVFLGAI
                     LGIGISIVPLYIVIFVSNGMIKGITDRYLETKTGHIQMSIPLDLSEENRVQRIETIEA
                     LPRVVSASYEIDGLGLAASTKCSTSAQIRGLDESITSDRGYREYIEIDAGDLFPQKEN
                     EVVLGRYLAKTLGVEIGETVSLMTLRETEGEEILPKLSIFRVAGIVSSGYRELDANWF
                     IIHSRVAQRILSSDMAYSYIGIKTKDPYGPDLDDIMNNVKKLYPEALVRPWRDIEQSL
                     FKSFSSTRSMLVFIMAIAGIVAAVNLSSVLTTFVMEHRKEIAVLRSFGVSRKQTMLIF
                     LLGGTVTGIIGCISGSAVGVAISLCINHLLSAIQTLIDFFTKLFSPSSQAITLLNPDY
                     YLERIPISMNLRYIVFILCSGILVSMIVSLIPAFRSSSIAPAELIRHD"
     gene            22609..23754
                     /gene="recA"
                     /locus_tag="PLQ30_01840"
     CDS             22609..23754
                     /gene="recA"
                     /locus_tag="PLQ30_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPW01215.1"
                     /translation="MEIQKEREDKLRALEAARLQIEKQFGQGSLMKLGAHTIAQGIEV
                     ISSGSILLDEALGIGGYPRGRIIEIYGPESSGKTTLALHAIAEAQKKGGIAAFIDAEH
                     ALDPIYARNLGVDINELYISQPDNGEQALDIAESLIRSGAVDIIVIDSVAALTPQAEI
                     EGEMGDSHVGLQARLMSQALRKLAGTLSKSKTILVFINQIRMKIGVMFGNPETTTGGN
                     ALKFYSSVRIEVRRIDTIEGGQNEEAIGNRVRVKVVKNKVAPPFRKAELEIIFGKGIS
                     WSASLLDAGVKCNVIEKRGAWYTWSGEKIGQGRENAKAYLEQNAEVAKEIETRVREIL
                     FNTDKSKTTEANTSQTNHSGTIDTFETATILENYEDNDTKIRTEDLF"
     gene            23791..24303
                     /gene="folK"
                     /locus_tag="PLQ30_01845"
     CDS             23791..24303
                     /gene="folK"
                     /locus_tag="PLQ30_01845"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPW01216.1"
                     /translation="MEKVWLGLGSNKGDSPSILDSAIQELAGFIIELRRSGLWRSRAR
                     YYTDQQDFLNLAICGFTDLTPRELLIQIHRIEANHGRDRSKEIIKGPRTLDIDILIYD
                     QKIVAEPDLIIPHPGLAERKFALLPLLELDYELIHPGLGVSLQELAACLPPQGIYPVN
                     DNRYDWPYSE"
     gene            24300..25097
                     /locus_tag="PLQ30_01850"
     CDS             24300..25097
                     /locus_tag="PLQ30_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HPW01217.1"
                     /translation="MTKELDDDSIQEKNSLISNGRTFCLSDFEGPLDLLLYLIKKNEM
                     NINDIRISSITEQFLQCLDSDPSADLDEISEFYQMAATLLYIKSRGLIPHIEDEAEEL
                     DDPRRNLIDQLIEYHRFKKLSELMEQREMEVEWFVERKVAQRTLPFADNPDIDPWIPA
                     DSWDLLRIFASMIRNFSSERIIDLYEEVSINEKIALIYELLNKKGNFRFDDLISRNTS
                     AMDLACAFLAVLDSTKNKLISVRQHKLFGDILIIPYSTTEPQNERTA"
     gene            25081..25638
                     /gene="scpB"
                     /locus_tag="PLQ30_01855"
     CDS             25081..25638
                     /gene="scpB"
                     /locus_tag="PLQ30_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPW01218.1"
                     /translation="MNEQLDLDAALISAILFLENEPISEEKLAKISGLTLEQTSLALQ
                     RICSEFDQPIHGFAPIRREERWIFAPKLAFWDKLKDHYGKRNEMRLSRAAMETLAIIA
                     YSQPITRAEIEAIRGVNVDAMVRLLLEKNLIAEISKRDVPGKPMQYGTTKEFLRCFGL
                     NSIEDLPKLDEVENSRFSRLETPEG"
     gene            25635..26393
                     /locus_tag="PLQ30_01860"
     CDS             25635..26393
                     /locus_tag="PLQ30_01860"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPW01219.1"
                     /translation="MSESIEKPMRLHAYLASLGIASRRSCEEMIREGRVTVDGVKASI
                     GQKIICNQRIEIDGQPIIAAYPKKFRYILLNKPKGYLSSLSDPYGRPCAIDLISGEVN
                     ERIYNIGRLDQWSSGLLLFTNDGDLASKLMHPSGQMEKEYRIQTNVPIPDNFASSFMR
                     GIIIDGVRYRARSVHITGPNSALIVLIEGKNREIRRVLEHFELHTRLLERIRYGPICD
                     PGLAPGNWRELTVDEVQALKTLCNPNQKAKGKGQ"
     gene            26390..28799
                     /gene="rpsA"
                     /locus_tag="PLQ30_01865"
                     /pseudo
     CDS             26390..28799
                     /gene="rpsA"
                     /locus_tag="PLQ30_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707864.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
     gene            28832..30346
                     /locus_tag="PLQ30_01870"
     CDS             28832..30346
                     /locus_tag="PLQ30_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPW01220.1"
                     /translation="MASEIDSDRFRTLIDINAQLNSSYTNYRSLLKTIVESAARLVGA
                     EAASLALFDASTESLRFEIALGPRGTELQGKSVPAAEGIVGWVFKNRRSIIVNDAAND
                     PRFSPKVSREISYSTNSILAVPLKIKDKTEGVIELINKASHGIFSSEDLKWVELFAVQ
                     AGIAFDNARQYEKTEQELIYLQHKVQQGEGWHPLIFSSGVMRDRLELVKRIAPSDASV
                     LILGESGVGKELIAEQLHLLSKRSDGPFMRVNCAALPESLLESELFGHVKGAFTDAIE
                     NRTGRFEAANGGTIFLDEIAELPLKLQSKLLRVIQQKTFERIGSNETIKVDVRIIAAT
                     NRDIEKLVKNQEFRSDLYYRLNVLPIQIPPLRERLDDVPTLAEYFLKKFTRETNKGIM
                     RFSESAMQSMLSYGWPGNIRELENAVERAVLLAKGQVITADDLMIGSKSSIEDEQFGG
                     KDLREAITIFKKNYIESALERNRWNQTETARNLRIQRTYLSRLIKELNILQNKE"
     gene            30350..31066
                     /locus_tag="PLQ30_01875"
     CDS             30350..31066
                     /locus_tag="PLQ30_01875"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW01221.1"
                     /translation="MNQEEMQQEQHNKEEKKSSSERFVDFISKNRRIIIGLGIAIVLI
                     IVAIGIYTAVSGNIASASSRAMELADQKALQWSQETDEEKRAEIESSLIAELDSIAQK
                     WPRTLAAQRALLRKAALLSQKKEYAEAEKTALEAYRRNKKSYVAPIALELAAVAAEEA
                     GNTDAALEHYTLITKDYKKDNPAAAHAFFNLGRIKEAKGDYKGAIEAYNQLIASFGDS
                     EWAMLAKNRVIYLNAQGLAG"
     gene            31158..>31852
                     /locus_tag="PLQ30_01880"
     CDS             31158..>31852
                     /locus_tag="PLQ30_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01222.1"
                     /translation="MKGEGKEKGIRFLLQPIAALLIINIYIIMTSCGLPTTDYLYTPK
                     DFSSEGGALVLEHDTKNIDELSSIFLGYEIYYRIFDDEVAAGDSRNSISSMKSSNIIQ
                     TANNYKYFPLKHRKKSESGQFSLDISPLIKVSSNTYTKYYLNMKPQETWTLTYEDSEI
                     VIDSIVRNRSDTASSAEIADADFCLSSGYQVNDRDYAGSSSSPDIVYIVFFAFAYGFD
                     TSTMRNVYSDPFIL"
BASE COUNT         9387 a         6419 c         7310 g         8736 t
ORIGIN      
        1 attggaaaga aacttggaga cattctgatg cctctaaggg ttgcgatcac ttattctcgt
       61 gtcagcccgc cactatttga atcgatgcgg attcttggaa aagaggaatg tattgtaaga
      121 atcgaggctg caatagcata tctgcgggga gcttgaccta actattcaat ccaagcgata
      181 ttttgtttac tgtgcctctg aagcgcagca aggagacttg tatcatgtct gaatcaattg
      241 aaaagcctga atatctggat tttatccgcg aggcagttgc tgaagatttg cggatcgggc
      301 gatgcaaaga agtccgtacc aggtttccac cagaaccaaa tggatggctt cacataggtc
      361 atgccaaagc actttttgtg gattttggtg tggctcagga ctttggtggt aaatgcaatc
      421 tgcgaatgga tgacacaaat ccagaaaaag aagatatgga gtatgtggag gcaatcaaaa
      481 gagatataaa atggctaggc ttcgactggg aagacaggct tttttttgct tctgattact
      541 atgagagact atttgagttg gcctgtaaat tgatcaataa aggccttgcc tatgtcgatg
      601 atctggatga agaacagatt aaagaatatc ggggcacgaa tgtgccggat aagaataata
      661 ttacctttac tcctcctggt cgaaacagcc catggagaga tcgttccccc aaagagaatc
      721 ttgatctatt cgaaagaatg cgagccggcg aattccctga tggttcaaag actctacgcg
      781 caaagatcga tatggctcat cctaatctcc tcatgcgaga ccctgttatg tacagaatca
      841 gaagaattcc tcactatagg acaggaacaa ggtggtgcat atatccaatg tacgattttg
      901 cacacccact ctccgacgcc atcgagggaa taacccattc tttatgttct ctagagtacg
      961 agattcatag acccttgtat gaatggttta ttcaaaactg cgaggtattc cctagtcgcc
     1021 aaattgaatt tgctcgtctt aacctatctc atacagttct ctcgaagcgc tggctgcttc
     1081 aacttgtccg agggaaaaag gtcaaaggtt gggatgatcc tcgcatgccc actattgccg
     1141 gcatgcgtag gcgcggatat cctgcggcag ggatcaggga ttttttgggg agaattggta
     1201 ttgcaaagac tgattctcta gtggatattc agctgttaga gcattgtata cgcgaagaat
     1261 taaatgcaca tgcaaatcgt tatatggctg tcatgaatcc cgtaaagctt gttatagaaa
     1321 actggcctaa aggaaaaatc gaatggcttg aagccatcaa caatccggag gatccttccg
     1381 caggcataag aaagattcct tttagtggca ctctctacat agatcgagaa gattttaaag
     1441 aaactcctcc gcccaaatat tatagactct atcctggcaa tcaggttcga ttgcgctatg
     1501 gttatgttgt tacttgtacg ggttttgaaa aagatccgat cagtggagaa attgagagaa
     1561 taagatgtgt ctatgatcca aatacaagag gcggagacgc acccgacaat cgaaaagtaa
     1621 aaggaactat ccaatgggtc tgtgccatgc atgcagttcc aattgaagca agattatacg
     1681 atcatctctt cgatgcggaa cgccccatgg aagtgcctga aggtaaaact ttttttgata
     1741 atctctctga ccactctctt ataatcgaat ctagagcggt ggccgagcca gccatagccg
     1801 aaattggaaa aggcgagaca gtacaatttg agaggatagg atatttttgc aaggatctcg
     1861 aatcaacaag cgaccttgcc attttcaatc gtacggtcac tttgaaggat acttgggcaa
     1921 aaatagaaaa gaaacttcag aggaatcctt aataatttct tgatcctcaa tgattaagca
     1981 atcatattcc attaaaggag taaaaatcta tgtcagagac cgtggtaact atggaaaaga
     2041 ttgtttccct cgcaaaacgt cgaggttttg tatttcaatc ctcggagata tatggaggtc
     2101 tttccggggc ttgggactat ggccctcaag gaattgagct aaagaatagg atcgctcgat
     2161 tctggtggaa ggaaatgact cagatgcatg ataacatagt aggtcttgat gcggccattt
     2221 taatgcaccc cagggtatgg gaagcttccg gacatgttga aaacttcacc gacccccttg
     2281 tcgattgcaa aaaatgcaag agtcgcttta gagcggatca aatcccgccc gaaaatcttg
     2341 ctgccaaaaa atgcccggat tgtggcggcg agctgaccga tactcgcaaa ttcaacctta
     2401 tgttcaagac cactctaggc ccagtggaag atgaatccgg ccttgtatac ctgcgacctg
     2461 aaacggcaca gggaatttat gtgaacttca agaatgttca gcaatccaac cgcatgaaga
     2521 ttccgttcgg catcgcgcag attggaaaag cttttcgcaa cgaaatagtc acgaaaaatt
     2581 ttatattcag aacctgtgag tttgaacaga tggagatgca attctttgtt aagcctggta
     2641 cggacgaaga ttggttcgaa tattggcgtt cagagcgatg gagctattac caaaaaatgg
     2701 gcgtgaatat tgataaattg cgctggcata agcatggacc cgatgaatta gctcactatg
     2761 ccaaagatgc ttatgatatc gaatacgagt ttcccatggg cttcaaagaa ctcgaaggag
     2821 tccataatcg taccaattat gatttgagcc gccatcagca gtattcaggc aaggatctgg
     2881 aatatattga tcaagatagt ggaaacgaac gctatattcc atacataata gagacgagtg
     2941 ctggtttgac cagacagctt ctaatgctgc tatgcgattc ttacgaagaa caaaaaatat
     3001 ctgataaagg taacgaagat gattggcgca cggtacttca ttttcatccg gttttggctc
     3061 caactacggt cgcggttctg cctctcatga aaaaggatgg cttagctgaa ctagcccagg
     3121 atattcgcat cgaactaaaa gatgattttg taactgacta tgatcagtct ggtgccatcg
     3181 gcaggcgtta tcggcggcag gatgaaattg gcacaccttt ttgcgtgact atcgactatc
     3241 agacaaaaaa cgataatact gtgacagttc gggacaggga cagcatgctc caggaaagga
     3301 tatctcgggc taatctaacc tcatacatac aacataaaat aaaaaattat catgctccca
     3361 atagatcgga agtagcttgt agctaatagc taaagccaaa gatcataaaa tttccctcta
     3421 ataatgatat ttcagggagg ttctatgtat tctaatgatt cactaattca aatctaaatc
     3481 agcaaatcac cacttcatat ttgaagtggt gatttgccgt aactctaagg atagttcact
     3541 cgcctagaac ggcatccttg cagggtttgg ccacagtaat cttgataact tcccgagctg
     3601 gaatagtaat ctgttctcct gttgccggat tgcgtccaac cttggctttg cgatggacaa
     3661 ctttcataat cgctatgccc gggatggtaa acttcttgtt cttcttagct tctgaataag
     3721 cgagcttagc aaactcatcc agcaaaagat tgacatcttt cttagtaaag cctgtggact
     3781 ttgctaatga atcaataatc tcgaccttgg tcagtggttt tcctgtcgat ggcatattct
     3841 tccccttttt ttcgagaatg gcgactcagc ataataagga gccgtcgcaa atatgcatga
     3901 agcatattcc tgtttttatt ttaccacaat tatgtccctt gtctagattt ggcattgaaa
     3961 acttgtagta tttcttactt tttggaagga ataatcgctt tttggcgagt acaacaatag
     4021 aataacttga gcaagttggc aatttcatcc tatactgcaa aacttaaagg agcatttttc
     4081 atgaacagag cgctcgagat cttgaaagaa cgcggttttg tgcagcaatg cagtgattta
     4141 gaggggcttt ctaagctaat ggacgaaagg ccgattactt tctatatagg cactgatcca
     4201 acgggtcctt ctctgcacat agggcatatg gttccatttt ttgcaatgcg ccatctaata
     4261 ggtgctggcc acagaggcat agcgctctta ggaggtggaa cggcgcgcat tggagaccca
     4321 tcgggtaagg ctgagatgag aaagctcatt tcctatgaag aaatagatgc taatactgag
     4381 aggtttaagg cgcagctcga taatttcatc catttcgacg gcgaccatgc gctgaccgct
     4441 aataataagg actggttatc caacctcaat tacatcgaat ttttgcgtga tataggtcgc
     4501 catttttctg tgaataggat gctttccttt gaaagctata gaatgcggct ggagacaggg
     4561 ctttccttta tcgagtttaa ctatcaactt ttgcagagct atgattatct tcagctccat
     4621 aagcttcatg attgtcgatt gcagataggg ggcgacgatc agtggggcaa tattgtggcc
     4681 ggaattgaat tgataagaag agtagagggt gccgagtgtt ttggtcttac ttttccactg
     4741 gtaactacca gcgatggcag aaaaatggga aagaccgaaa gaggcgcact atttcttgat
     4801 ccagatatca caagtcccta tgagtttttc cagtatttta gaaatgtgcc agatggtgat
     4861 gtagagagat tcttgctaat gtttactttt ctgcctatag aagaatgcag atatctaggt
     4921 tcactcaagg acaaagcgct taacgaagct aaagagcgac ttgcatggga agtaacatct
     4981 cttatccatg gccaagaaga agcggataaa gcgctaagtg cagccggggc agcttttaag
     5041 ggagaaggag acgcttcaca acttccaact atcgaggtcg aaatatctcg gctggattca
     5101 ggcattggag tgctggatct atttgtagaa gcaggacttg cgccttctaa gagcgaagcg
     5161 cgcagactag tcaagcaaag cggcgcctcg gttaatggca gaaaaataga aaacgaaatg
     5221 cagaccttga catcgagcga tttaatagat ggcgcactca tgctcaaagc tgggaaaaag
     5281 cgtgttgcgc ggatagtgcc aaagttataa gaacagcatg cagcacctat ttcttgcact
     5341 tgcggatatt catggcaata tcaatgctct ggaacagata ctcaaggcaa attccaacat
     5401 agaaggaatt tttgtagcag gagaccttac gaatttcgga aaagaaaaag aagcccaaag
     5461 agtgctctta gctttttcga gcattgcacc caaggccaaa ctctattttt tggccggaaa
     5521 ttgcgatacc gcagaagcaa ggaagtgctt tgatatgcaa ccaggctata ttgaacagcg
     5581 atgctgcgcc cttctacctg agaaggagat atggatcatt ggttgtggtg gaggtctata
     5641 tcatactggc ctgacacctt tcgaaataag agaagaaggg ctggaaagcg gaataaagca
     5701 agcccttaaa tgcgctattc gaggttatag agatatttca agtatggatg gttcagagcc
     5761 cactccaggt ctcattgttt tgactcacac tccgcccaaa gacacctttt ccgacatgcg
     5821 tcattcgcgt catataggaa gtgcagcttt ccagacattc ctgtatgagc aggagcctct
     5881 tctatggatt tgtggccata tccatgaggg taaaagcatc catattgaaa ataagacctt
     5941 agtggttaat ccagggccgg ctgcccatgg tttgtattcc ctaatcctcc taaaaaaatc
     6001 tcctaagggc tggaaagccg cagcagaact aagatcgata tgatattctc gccatttatc
     6061 accgaccggt aggactataa agatatttga agtagtccat ccccgtagac agcgtcttat
     6121 taaatgcagg cattgtcttg cgatatgact cgattgcttt ccagaattcg aagaatgatg
     6181 gatctctgcc aaaggaatcg gcatagatct tcgaagcttc ggcatcggcc tttcctttta
     6241 tagattcacc ttttgcatac gcagaagaca aaatggtgcg cttttcattt tccaatttgc
     6301 cgagccattc ggctttcttc ccctcaccat aagctctgaa cgcttgtgct atttgatttc
     6361 tttcctttat cattcgctga tatacacttt cgctaagttc atcggagtac ttgatttgcc
     6421 gtggaaggat atccacaact tcaataccaa aagaaggaac tatgtctgag acaagcgctc
     6481 tcatttcttc tgtcagttcc aggcgcccct tttcgatttt ttcgtattgc gttggtgtta
     6541 ttgcgagctg ctgaagagtt gcactgtttt ccacgtctcc tgtttgaaaa ctctctacct
     6601 ggttttccga aagaatttca tcgctgctac gaaccgcctc tcgaagataa ttcgaagaga
     6661 taacagttct caccgccgaa tcgatgacat cgctaagctt gctatatgct gcgttcaggt
     6721 cattcacaga ttcatagaat tttattggat ccgcaattct ccagcgagcg gtagtatcta
     6781 catagataaa ttgattttcc ttagtgggaa ttctctgggg ttctccatcc caggacataa
     6841 gtctcttagg atattttatg acagtatcga taatagggct gcgccatttt aatccggcat
     6901 cgctatgaga gttgactatt ttaccaaaac gtactactac ggcttgttca ccctcattta
     6961 gcacaaaaag tggaccaaag atcacgatga ataaaatagc taatgccaca acaaccgtta
     7021 ttattattga tgcttttttc atcttgaagc ctcctgagaa gaaggagtct gggctagatt
     7081 ctttattggc aagagattct gaagcgattt atcgattagg tccacatgct cttggtcctt
     7141 aaatatctcc tccatcattt cgtaatatag acgctttcgt gttacttcgg gcgccttgcg
     7201 atattcttca tagacggaat tgaaacgaga gacatcaccc tttgccacat tgacacgctc
     7261 tgaagcatag ccttgcgcaa cttctagtac ttggtccgct tgaccttgta cctttgggat
     7321 ttctgcgtta tatgcttctc ttccttcatt gataagcctg ttcatatcct ggatcgcctt
     7381 gttcacatct tcaaaggccg cttgaacgcc ttccggaggt acaatgtttt gaagctgaac
     7441 ttgtgtcacc gtaattccaa gaccatatat cgaaaagaat tcgttcataa gggtaaccgc
     7501 ggtttcctga atactctggc gctcaggccc tatgactccc agaatggtcc tatcaccgac
     7561 aagcatgttt agcaccatct gagaagtatc tcttattgtt ttttctttat ccatcacatt
     7621 gaacagccag gcctttggat ctgtaatacg atattgtatg atccactcga catctacaat
     7681 attgaggtct ccagtcaaca tcgttgattc atctgggaat ttttcgttcg agtattgggt
     7741 aatgatgcct ggttttacgg ttctaaagcc gaaagcttcg gtttgaacca cctgagtttt
     7801 taccagatgg gctttttgaa ttccaaaggg cagcttgtaa tgaagtcctg ctccaagggt
     7861 ctttgtatac tttccaaagg ttgtaatgac agctttttcg gtttggtcta ctacgacaaa
     7921 gcttgtcagc aaaaagacta agactgctaa agctacaatt atgattatta caatcggcca
     7981 ttttagtttt acattcagtt ttttcttagg attgataata tctgccatgt tttgccccct
     8041 tctcaggaaa tagattattg tcacatattt ctaatgattg ttctatccta tgagaaatca
     8101 gagcatcaac ttcctcatga tctatttgga acaaatcgga aacagcaagg ttaaaatcac
     8161 caggaagagg gtcaaaaata gtcaaaggca tgtcttcaaa aataggttct ggaaagccta
     8221 aacaacatga atgcaaaaaa taactgctga ttttggctgt cttagatcct ccatattttg
     8281 aatcgccgac aagcggatag ccatgagatg ctagttgtgc tcttatttga tgcgtaagtc
     8341 ctgttatgag ttcgataagc aaaaaactat atgacttatt atgcatcaat ggagatagat
     8401 aaaggctcgc ttctttgcct tttctatcat ctaataggct caattttctc tttttatcgc
     8461 gcagaataaa atcctttaac gagataggtt catctatctt tccttgaact attgcagcat
     8521 agaacttgcg aattcttctg ttcttcattg caacactgaa aagttccgcg cctttcctgg
     8581 tttttgagaa aaaaatgatt ccagaggtgt tcctatccag tcgatgcagt ggtcctggag
     8641 aaaatgacgc agatggctcc aattgatcat agaggattct ctgcacataa gcattgagac
     8701 tagagcttcc atcgtgtacc aatattcctc gcggcttatc aaaagcaata agttgggcat
     8761 tctgccacaa gatgcgggga atttcggaaa aagccacctg tgaatattct tttttaggaa
     8821 gagtactttt tattatggtt tggttttgac cattctccag acttctttcg gagaacttta
     8881 tttcgatgac atctccttct tttagtctat cattcggcgc aataggcata ttcgaaatcc
     8941 ttatatctcc ctggcggaaa aggcgataga ttgtcgaaag agccgtatct ttgagcacct
     9001 ttctgactat tctgtcagcg cgccgtccat cgtcatccct gctaagggtg atgatcaaca
     9061 atttgcccac gaattgacca taatgttttg gagctggttg gtcaacgact gcctatgctt
     9121 caatcatgct tgacagagcc ctaagcatct tacacactct acataatacg taatcaggct
     9181 atatagttct taaatgccag aggagtatat ttcttcgatg cagaacttca aagtggtaat
     9241 aggtatgtat aataatctgc cctataatgc gtcgggagca ttgtatgaac agacatatca
     9301 aggcgcctgg cgcccttttc tttccggact atacaaattt ctctctatta aatcggcaat
     9361 ctacttttct ggtgctattt tcccatggct tgaagagaac catcccgaat atatgtacct
     9421 tctcgtcgag atggttagaa gaggtcaggt cgaactttta ggaggcggat tgtacaaccc
     9481 gctttcaccc cttataagcg cgcaagatat gaccggacag gtcgaaagtc taagcgcttt
     9541 tattcgcaag acaacaggca aaaggccttc tggcgcatgg ctctacgaat attcatggag
     9601 ctcttcactg ccatccattc tacaaaactc aaaaatacaa tatacttttt tgcctgcgga
     9661 gtactttccc aacgtagagg gaggcatgga atttggcttg ccggttactt cggaagatca
     9721 tcgaaaggta ataaccatat ttcccacttt cgaatccaag gactcagaaa acagctttat
     9781 gccttatgag aatactctga cggatcttca agagtcgtat cctgaatgca agactttcat
     9841 cattatggcg gatggcaacg aaataagcat gagttgggaa aagagcggcc ttgaatctcc
     9901 tgatgttctt ttcgagcgta cctttgcatg gtttcagaag aattgtctag aatatgatac
     9961 ccttactccc attcaactcc ataaaaccac aaaaccctct agaatagtat atttctctca
    10021 atgttattct cggcggttta aagaatattg caaaaagacg atcaatcgct tgcctattca
    10081 aaatcaagaa tatatacaga tttctaaaca atctgtatta gatcatccac ttgtgcatgt
    10141 tctttatcaa aaattgaact atgtatcaac tatgatagct ctttttcgag gtgataaatc
    10201 gagaaagaaa gcctctatag atgatatctg gaaagctcaa tgcggtgatc tgttttggat
    10261 cggaccctgc ggtggaatcc ttattccgga agctagaatt gcggcttttt cctctcttat
    10321 agaggctgaa aagacaataa ggcagagtag atttcgccct tatctatcat tcgacgacct
    10381 caatttcgat ggcttaaagg aagcaatatt tcaatcttct gtttacaact gctatctgca
    10441 atcggggtcc gcctcggtaa gcgaattcga ctccataaag acaggaacaa actatgcttg
    10501 cggttggaat gacgatcaat tctcgacagg ctgcttcaaa gattacataa aaaccgaggg
    10561 gaacttcgaa agagatctta tagcagtaga gcgttggaat atggtagaaa atccaagaga
    10621 agagtcgagc gttctttttc gaagagaatt ttcggctaga tttgaagacc gattattgat
    10681 gcttctttgt agaaagactt atcgcttcag aaatgacttt ttcagtatcg actacgaatt
    10741 gagcaataag aatacggaag cttgcctatt tcgtttttgt accaattcag agattatagc
    10801 tgctccgatt tttgaggatc atcgatttga attgctgcat catcgcgaga gcaaggcctt
    10861 agatttgaga tctcaagctt ttttcgaaac ggttgatggt atcgggctta gcaatgttag
    10921 aaaagcagaa agagtgttga ttcgttctga tttgccattc tccctattgg caaaacctaa
    10981 tttccttaag aagccttcgg tctctccgca agttatggat gttccatcag attctctcat
    11041 gttcgaaggc ttttcgatcg gtatcggatg ggacctgtct attcctgctg agggaacagc
    11101 ctttttttct ctctctgtcc atctagaaca ttgaggaatt agccgatata tgtatcttcg
    11161 atttagcgag gtcttgaggt tttaggatga atttctttaa aataggagaa taatggagat
    11221 gattcaattc cccgataagc tggaaggcct tcatattcac cttgtgggag caaaaggcac
    11281 cgggatgacc gcacttgcgg agatacttca aaagcgaggg gcaatcctta cgggtagcga
    11341 tgtggcagat gtgttctata ccgatagaat tctacactcg cttggtattg agctttttga
    11401 gaacttcgac aagaagaatc tacctatcag cactgatata gtcattcact ccgcggcata
    11461 ttcacgcgaa ggaaacccag agctcaaggc agccacgcat cgtggtatac ccattttcag
    11521 ttatcctgaa gtacttggag ccctttctct gcatagtagc tctgcaggca tagccggggt
    11581 tcatggaaaa accacgacag ctgctttggc tgggatactc ataaaagcat taggactgcc
    11641 cgcaacagtg ctggcaggtt ctgcgatttc taatttcgac gatcgatgta ctctggtcca
    11701 aggagagagc ttttttatag ctgaaacttg cgagtaccgc aaacattttc tcaacttcaa
    11761 gcctacttgg atagtactca catcggtgga aagcgatcat caagattatt ttccaacata
    11821 cgaaagcata cgcgatgcat ttgtggaata tgtcctcagt cttcctccca atggtgttct
    11881 aatcttttgt gccgatcaga agggtgctgt agaggttgct gacattgtcc tacaaagacg
    11941 aagagatatc aaatatgtag agtatggttt tttggctcat ggaaaatatg gaattcgcga
    12001 ttttcagacc aaagctggag ccagttcatt tatccttgga gagagttctt ttccaataag
    12061 gattcatttg ccaggaaggc acctggtcca ggattctgtt gcagctattg cacttgtcga
    12121 acagatattt gaaactgtga aatcgagacc gtttgaggaa tcagagtgga tctcagtagg
    12181 tgaagcattg gctgcattta ggggttcaaa acgacgcagc gagataatag gggagtacaa
    12241 aggaatactc atcatggatg attatgggca ccatccaacg gcaattaaaa ctacaattga
    12301 aggaataaaa aaattctggc ctcatagaag acttattgtg gatttcatgt cccataccta
    12361 caccaggaca attgccctgc aggatgactt catttcctct ctcgatggtg cagattctgt
    12421 tttattgcac aaaatctatg cttcagcgcg tgaacggcct gtctcggggt tcaatggtag
    12481 aactctctat cagaagcttt gcgagcatcg cccagaactg tttccaatgg acttagaacg
    12541 agatactagg caaaaggaaa ttgcctatta taagaataag gctagccaag atcagacagg
    12601 ctttgttgcc tattccgatg agccgcttaa agcgctagat gctctttcta atctgttgca
    12661 gccaggagat gtttttttga ctctgggcgc tggtgataat tggaaactag gtaaggctct
    12721 tgctgaaaaa atagaaaaga atggataaga agccttaaca tggaagcaat tgaggaatca
    12781 ggagagcaga atcaatgaag agtatgaccg gatttgccca tagtccaatc ttggaaaaag
    12841 aatttcaagg tactcttacg ctaaaatcct tcaataatcg ttttttagac atatcgattt
    12901 ttcttccatc atatgcatct cgaattgagc ccctcattcg agatttttta ggatcacgca
    12961 tacttcgagg caaggtcgaa tgcactttca agctaagaaa aataaagttc tcacttgaag
    13021 atattgagct tcaatttcag gcagccactc ttctttctga acaattgcgc aagcttgccg
    13081 aagcatgcgg tatagaaaag gatttatcgc tcgaccttat tacaaaattt ccaggaattg
    13141 tcgatatgaa cacagagatt gaagaggatt acctttggcc tgtattgatg ccaatccttg
    13201 aagaaacctg gctgaaattt gaggagagtc gaattcgaga aggtacagcg acagaagaga
    13261 atataagagg ccagcttcag agactgaaat cgaagcttat tgaaatcgaa ctccaagcgg
    13321 attcgctcga gagaatggtg tatgaccagt tagtagccag attcaaagag ctattatccg
    13381 aaagctatga tgaagggcgg gtacttcagg aagttgcggt gcaacttgtt cgccttacta
    13441 tcaatgaaga aattggtcgg ctcagagccc acattgatgc ttttgaatta atggcaaaag
    13501 aaccagcctg tggcaagaaa cttgattttc tatgccagga gatgaataga gaaatcaata
    13561 cgataggttc aaagagcatg cttgtctctg tatctcatgc ggtggtcgaa atgaaagacg
    13621 ctctcgaaaa tattagagag cagctcagaa atgtggagta gaataggcat gggtatcatt
    13681 gcgatatcgg ggaaatcagg atgtggtaac tcaaccgtgt tacgtctggt tgctgagaaa
    13741 ctcggttacg aaccagtgaa ttacactttt cgcagcatgg cagccgacat gggaataagc
    13801 ttccaagaat tgcataagaa agcgcaagaa aatcttgaat ttgatcgcag gctagacgaa
    13861 catcaggttc tattggccag aaaggggaat accgtgatcg gctctagact ggcaatttgg
    13921 atggtgcctg aggcagatct aaaagtctat cttaaggcca gccatgccgt gcgtgttagg
    13981 agaatccatg ccagagagga tggcgatatt gaagccatag atcaatttac aaaagagaga
    14041 gatgccaagg atagaggcag atatcttgcg ctctacaata tagatataga cgatgtctct
    14101 aatgcccatc ttgtaatcga cactgagcga tggtctgcgg atcagattgc cgatatcata
    14161 gtggacgcat accatctgat tcctttaggt gacccatatg gaagcagcct ctgaaaataa
    14221 gcgagttgcc tttgtaactg gtgcaacgag tggccttggt ttagcgatct caagatcact
    14281 gattgatatt ggatttattg tctatggagc aggacgccga aaaccccctc tcgatctacc
    14341 tgataattat gtctatgtgc aaaccgatgt ggcactggat gaatcggtta acaagagcag
    14401 agattttgtg ctttctcagc aaagccacat cgatctatta gtttgcgcag cgggttttgg
    14461 cataagcgga tcgattgagg atactcctat agattcagct atagatcagt tcaatgtgaa
    14521 ttttttcggc gttgcgagag tggtatgtgc ttttttgcct ggtatgcgcg agcagggtag
    14581 gggtcgaatc atcattatcg gctctatagc aggaaagacc ggaatgccat tccaagccta
    14641 ctattccgca agcaaatttg ctcttgaagg ttttgtagaa tcgctgagat acgaggtgcg
    14701 cagattcaac atcgaagcct gcattgtgga gccaggcgat ttcaaaactg ggttcactgg
    14761 ctcaagacaa aagatcattg cgcccgaaag tccttactat aatcaatttg agagagtaat
    14821 aagtgtacag gaacatgatg agatacatgg attggatccg gcaaaactgg gagaaaggat
    14881 tgcccgattg gccgtatcga agcgtcttcc tgcacgcctt accatagggc ctttatttga
    14941 acgtttggcc gtatggattc gaaggatcct tccagatgct tggttcgagg ccttttatag
    15001 aatgtactat catctttagc atagagatct cgagaattct tatcttatgc gctatgatag
    15061 agctctctct atggacgcat tatatatagg ggaaatatat gcatgttgcg acatcatcta
    15121 tagaaaaaaa gcattttggc acactctcta gcggagagga ggtctctctt ttcattctca
    15181 aggccggatc ttttcaagca agctttactg attggggagc aacatgggtc agtttaataa
    15241 tgcctgacaa ggaagggcaa aaggacgaca tattgcttgg tttttcaact ctatctggtt
    15301 atgtgggaaa gcatccatat tttggttcaa caattggtcg ttttgcaaac aggataggcg
    15361 gcgctcggtt tgtacttaat ggaattggct acaaactctg gacaaacgac aattgcaatc
    15421 atctgcacgg gggtcgcaag ggctttgaca aatttctatg gaattatgaa ttatcagcat
    15481 ttgatggaga gcctgctatt agattttcga gaactagccc tgataaggag gaaggatatc
    15541 ctggcacact ctatgccgaa gttattgctt ctctatcttc tttaggagaa ttgcatttgc
    15601 attattatgc tgagacggac gcaatgaccc cggtcaatct aacaaatcat gcatatttca
    15661 atctagcggg cgaggggaaa gggacaattc tagaccatga gcttgaaatg caggcaggta
    15721 tgtatttacc agtagatgaa tacctaattc caaacggaga gatttgttct gtagagggta
    15781 cggcccttga tttcagatgc ccaaagcgaa ttggcagaga tatacaagaa gtaggcaatg
    15841 gctatgattt ttgctatatc atcgataagg atcgcttgcc acttaaaacc tttgctattg
    15901 taactgagcc atctggtggt cgaagcctta gtatatcgac cacattgcca gccgttcagt
    15961 tttatacagg caacaacctg gcaggaactt ttggcaaaag tggctctatc tatgacaaat
    16021 atgccggttt ctgtcttgaa acagagatgt atcctgattc accaaacaaa caaaattttc
    16081 cttcttgtat tctgcttccg ggtatgccct gggaacatga aacagtatat aaattcagct
    16141 tacaatgtta attaaggagt tgcatatgct agaagatatt tcaaaaccaa tagaagacat
    16201 aaaagaggac attcttgaaa tctgggggcg tctttgacgc gcctgcaata gaagataaga
    16261 ttcatgattt agagggtcta gccagcgctc cggatttctg gtctgagcag acacaagcca
    16321 agaaaattct cagcgagata aagatattga gggatcgctt ggaaacctgg caaaccatgc
    16381 acaatgatat tctcacactt gaagagatgt acgaattagc acgtcaggag gatcagcaag
    16441 atatggaggc cgatcttcga gtcagtcttg aggagataag aactcgcttc gacagggcag
    16501 tggtgatcga aatgctctct ggagaagcag atcgaaacga tgcttatctc accattcatg
    16561 ctggttcggg tggtaccgaa gcatgtgatt gggcaagtat gctctatcga atgtatgtcc
    16621 gatgggctga aaggcatgat tttaaggtgg agactctcga cttcttggaa gcagagggcg
    16681 gaataaagtc tgtgacaatc caaatatcag gtgaattcgt atacggttat ctaaagggag
    16741 agacaggggt tcatcgtctg gtaaggattt cccctttcga cgcgaatgcc aggcgacaca
    16801 catccttcgc ctctgtatcg gtaatgccag ttctcgatga gtcgatagaa atcgatataa
    16861 gaccagaaga tatacggata gatacatatc gagcaggagg agcaggcggg caaaaagtca
    16921 acaagacaga ctccgcggta agaattacac atctggctac gggtattgta gtaacatgtc
    16981 agaatgagcg cagccagtat aagaataaag atgtagccat gaacattctc aagtcaagac
    17041 tgtatgagta ttacaaagca gagcgcgaca aagaaaccgc taaatttgct ggcgaaaaaa
    17101 aggagatcgc ctggggttct caaattcgat cttatgtctt ccagccttat accatggtaa
    17161 aggatcatcg gaataaattc tcggtaggaa atgttcaagc cgtaatggat ggtgacctgg
    17221 atccattctt ggaagcctat cttcattggc aatggaaagg gggtacatct gttgaagagg
    17281 aagatgaaga catataagga tcttttccta tttccgctat ttctttttct cctcatagac
    17341 actagcctat ttgcccagat tacgccatca cagaataagg gcctgggctc tattgcactt
    17401 gtttttccta tacaagtctc gaatttgcct gatttcgatc cattaggcca agaaaacctg
    17461 gattcgataa tgatattaga atttatacag gataggctta gtgatcccca gtattatgaa
    17521 aataagcctt ctccctcgga ggcaaacagc ctatcaaggc tgatggagga aaccctgagg
    17581 ttgcgcacaa cttctgaaga attaatagca atagatatcg aaactctcaa gaactggaat
    17641 tggaacagga ttattcagga gaaatcggct ctctttcagg aaggttctct taagaaatca
    17701 gatgcagttc tgtttatgat gtatacgatt gaagacagta atatgcaatt agcatccttt
    17761 ctgctaaaaa atacagggga aagctatgct tttttatcaa acaggttttc ggtctttgat
    17821 ttgttagatg cagccagaaa gactgttgac tcttttatag aaaaaggtgc acttcttgcc
    17881 aagaagagta cacccgtcca atctgaatta tcgatcgaag aaatcgaatt gccttctatt
    17941 gatccatgga agaatagaga taattacaag aaagcccaat cggcctttga gacttccatg
    18001 ggaggggctg caatcagtct gtcgatttcc tttgttgccg tgggcttgtg gaatatatat
    18061 cgcgaggctg cttacagaaa tagtgcgttt gatgttgcag ctacgatctc tgctatagct
    18121 gctgggacga gtgccgcagt caccgccaca tttttaacta tatcaatatg gaatgcaggt
    18181 aaaatgctcg gagcatctca ctgaattact tgcacagcta ggagaaataa tgaattttaa
    18241 ggacaaaatc aaagccctat ttcacagaag tcaattgagc gaggaagtat ttgaagagtt
    18301 ggaggatctt ttgattgagg gtgacattgg tgcctcaatt gcttttgaag ttgtggatgc
    18361 gcttaaagac acctgcagga aagataatat ttcaacccca gaagaggcca gaaagggatt
    18421 aaaaaaaata ctgcgcccct ttatcagagt cgaagatttc aacttagatg ataagcgtct
    18481 caatattgtt ttaatacttg gtgtcaatgg tgtcggaaag acaacaagtt gcgctaagct
    18541 ggcaaaatgg gcggaaaaca aaggaataaa tcaacccata atacttgccg cctgtgatac
    18601 atttcgtgca gcggctattg aacagcttag gattcatgga caaaggctag gagctcgcgt
    18661 aatagctcaa cagcatggat cggatccagg agcggttttg tgggatgcaa tagatgccgc
    18721 caagaaaaac aatgcgaatc tcgtcattgc cgatacagca ggacgaatgc atacgagagc
    18781 cgatcttcta cgtgaacttg aaaaactcga tcgtgtagta agccagaaag caggagatgt
    18841 caactaccgc aggcttttgg tgctggatgc tacgacaggg caaaatgcca tgcgacaggc
    18901 agagatattc aatggctcgg tcaagataga tggcctaatc atggcaaaat acgattccgc
    18961 ttcaaaggga ggcatgataa ttgccgtaag caaacaattc ggactaccta ccgtattcct
    19021 tggaacaggc gagaagtata atgatttcga gccttttgat atcgataaat acttagatga
    19081 ttttgtcgga gactgaggta tatagcttcg atgatcaaga cttcggatga aaaaaaacag
    19141 cgatggatgc tgtcaggaat gcgttttagg ctgccactgt acattgccat ccgatggtcg
    19201 ataacgggga aaaaatccgc ccacagctca ggattgaaat ttgcttctct ggggattgct
    19261 gttggagtta ttgcattaat agtggtattg agcgttatga atggtctcca actgcaatac
    19321 atagaatcac ttcttgagac aacgagcttt catattagag caagaattga agaaagcaat
    19381 ctcgatatat tcacagacac cctgcgaaaa aatcgactgg taaaatcggt gactcccttt
    19441 atagagacga atctcctcgt acaaagctcg gaggatcgac agaacgtgct tcgggtgatg
    19501 tggatcgatc ctgaggattt agctctagat tccggttatt gcaaagcctt gaatattctt
    19561 ccttcggctg cggccaagag cctggaggag ggaataatac tgggttccga agcagcacag
    19621 atcttaggag tctctgaagg atctatactc actctaagag gagcctttgt agatcccgaa
    19681 gaggggattc aagggtattc cgaaagagtc aaagtctcga gcattttccg ctccgggtac
    19741 tacgagctcg atgctgcttt cgctattatg cccagactaa aagctaagga cctggcaggc
    19801 tttcctgcct ccaccattct tggaatcaaa ctgataaatg cggaaaaatc tatttccttt
    19861 gtctctatgc tcgagcagga acctggaata atcgatatcg aatcatggca gaactataat
    19921 cgcagtttct tcagcgctct tcatacagag aagatattga tgttcttatt ggttggcatt
    19981 atttttttgg ttgtagctat caacattcat tactcgatgc gtcgtagcat agccaggaaa
    20041 tcgagggatc tggcgatatt ggcagccatg ggaatgaatc agcattcaat atcgagcatt
    20101 tttatgttag aggggattct ggtaggtctg gcaggtgctc tatttggtat tgctatcggt
    20161 attccaattg ctcagcatgt cgatggtatt ataaacggcg caattgggtt gatcgaatca
    20221 ttcatctctt ttctttatcg cctcgggttt gttaagggag tcccagatct caggattttc
    20281 tctccctcga tattctatat tgaaggtatt ccatctcgga tattcatctc cgatatagct
    20341 gttattgcag cttttgcact gattttccct gtcatcgcag tttatctagc ttaccgtcgc
    20401 tttagaaatg tatccccgct ggaggttctg agaagtgagt aatccaataa tagaatgcag
    20461 ggatctaacc aaaacctttt cttcgagtgc tgaagtattg tgtattctcg atcgggcgaa
    20521 ttttgcgatg aagagaggcc aaatatgtac aattcttggt cctagtggat caggcaagag
    20581 cacttttcta gctatcctag gcagccttga gcgcttcgat tctggagatg tgcatgtcga
    20641 tggctatgat ctatcctcgc tctcggaaaa atccctttcc atgtttaggc gaaacatagt
    20701 cggatttgtt tttcaatttc acttcctgct caatgatttt actgcgctag aaaatgttgc
    20761 tctgccaagc tatgtagcgg gcaccccaaa aaacgaggca tgggataggg ctcggagact
    20821 tttggatatg gttggacttg gcaagcgtct ggatcatttt ccatcacagc tctccggtgg
    20881 agagcggcaa agagtcgccg ttgcaagagc attgatcaat tcaccatcga ttgtccttgc
    20941 ggacgaaccc actggaaatc tcgatgcttc aagcgctaaa gagttgaggt ctctgcttca
    21001 aaatcttcca gaatcggcag ggacaactct tttgatagtc acccatgatc ccgatttggc
    21061 agccattggc ggatcatcct acctcctgaa aggaggtcga ttcgaatgca taaaataaga
    21121 ctgagtgggc ttaagccatt ctcttatctt ggatttcgta tgctgttggg aagacctgct
    21181 gcagccagag aaaaaagtgc gcatagcgct cgccatggtg tttttcttgg cgctatcttg
    21241 ggcattggca ttagtattgt gcccctttat atcgttattt ttgtttctaa tggaatgatc
    21301 aaaggcatca ccgaccgcta tctcgaaacc aagacaggtc acatacagat gagtattccc
    21361 ctcgatttga gcgaagaaaa tagggtacag agaatagaga caattgaggc acttccccgt
    21421 gttgtatctg catcgtacga aatagatggc cttggacttg ccgcttcgac caaatgctcg
    21481 acttcagcgc agattcgggg cttagacgag tctatcacat cagatagggg atacagagaa
    21541 tacattgaga tagatgccgg ggatctattt cctcaaaaag agaacgaggt agttctagga
    21601 cgctaccttg caaaaactct tggtgtagag atcggagaaa ctgtctcgct gatgaccttg
    21661 cgggaaacag aaggggaaga aatccttcca aaattgagca ttttcagagt agctggaata
    21721 gtgtccagtg gttatcgcga acttgatgcc aattggttca taatccattc ccgagttgct
    21781 cagcgcattt tgagttccga tatggcatat tcgtatattg gaataaaaac aaaagatcct
    21841 tacggacctg atcttgatga tataatgaac aatgtcaaga aactatatcc agaagctctt
    21901 gtgcggccct ggcgagatat agaacaaagc ctatttaaat ctttttctag cactagatct
    21961 atgctagtat tcataatggc tattgcaggc attgtagcgg cagtcaattt atcttctgta
    22021 ctgactactt ttgtcatgga acatagaaaa gagatagcgg ttttgcgttc ttttggtgta
    22081 tctcggaagc aaaccatgct catttttctc ttgggaggga cggttacagg cattataggt
    22141 tgcatatcag gaagcgctgt aggtgttgca atatcccttt gtatcaacca tctactctcg
    22201 gcaattcaaa ccttgatcga tttttttacg aaattattta gtccttcctc tcaagcgata
    22261 accttactta acccggatta ttatcttgaa cgaatcccga tatcaatgaa tttgagatat
    22321 atagttttta tcttgtgctc aggaatacta gtcagtatga ttgtttccct tattcccgca
    22381 tttagatcgt cctccatcgc acccgcagaa ttgatccgcc atgattgaat atagtgatga
    22441 gtggctgcag aattttcaat cgtgtgatac aatttgtgcg tcatagagga tgaaggggta
    22501 tttgcaatgg cgaagagaca aaaccgctca gcggcagaat tgatgacaga acaggaacct
    22561 aaatttggac aaaaaacggg tgcggtagga gaaaaaaagg atccagatat ggaaatccag
    22621 aaggaaaggg aagacaaact gagagcattg gaagcagcca gattacagat tgaaaagcaa
    22681 tttggccaag gttccttgat gaaacttgga gcccatacga ttgctcaggg aatagaggtc
    22741 atctcatcag ggtcgatcct tctggatgaa gccctcggta taggcggtta tcctcgcggc
    22801 cgaatcatcg agatctacgg tcccgagtca tctggcaaaa caacgctagc tctgcatgca
    22861 attgccgaag ctcagaagaa aggcgggata gcagcattca tcgatgctga gcacgctctg
    22921 gatccgatat atgcacgaaa tctaggtgtc gatataaatg aactctatat ttctcaacca
    22981 gacaatggcg aacaagcgct ggacatagca gaatccctta tccgttcagg tgccgtggac
    23041 ataattgtaa tagattctgt ggctgctctc acacctcaag ccgaaattga aggtgaaatg
    23101 ggcgattccc acgtagggct tcaggctaga cttatgagtc aagctctgcg aaaacttgcc
    23161 ggtactctct caaagagcaa gacaattctc gtttttatca atcaaatacg catgaaaata
    23221 ggtgtgatgt ttggtaatcc agaaaccacc acaggcggca atgccctcaa gttttattct
    23281 tcagttcgca tcgaagttcg aaggatcgat acaatcgaag gaggacagaa cgaagaagca
    23341 atcggtaata gggtgcgcgt caaggtggtc aagaacaagg ttgcgccacc attccgcaag
    23401 gctgaactcg agatcatatt cggtaaaggc atctcttgga gtgcttctct tctagatgca
    23461 ggagtaaaat gtaacgtaat cgaaaagaga ggcgcttggt atacctggtc tggcgagaag
    23521 atagggcagg gcagagaaaa tgcaaaggca tatctggagc aaaatgcgga agttgcaaaa
    23581 gaaatcgaaa cacgggtccg cgaaatatta ttcaacacag ataaatctaa aacaacggaa
    23641 gccaatacct cacagacaaa tcattccgga acaattgata cttttgagac ggccaccatt
    23701 ttggaaaatt acgaagacaa tgacactaaa atcagaacag aggatttgtt ctgaagccat
    23761 aatctaaaag gagctttagc gcttgctatc atggaaaaag tatggctagg actaggctca
    23821 aataaaggag attctccttc tattttagat tcagccatcc aagagcttgc aggctttata
    23881 atagagcttc ggcgctctgg actttggcgt tcccgcgccc gctattacac agatcagcaa
    23941 gattttctta accttgcaat ttgtggtttt accgacctta cgccgcgcga attacttatc
    24001 cagatccatc gtattgaagc aaatcatggt agagatagaa gtaaagagat cataaaaggc
    24061 cctcgaaccc ttgatataga tattcttata tacgatcaaa aaatagtggc agaaccagat
    24121 ttgataattc ctcatcctgg tctcgcagag aggaaattcg ctctattgcc tctcctagag
    24181 cttgattacg aactgattca tccaggtctc ggtgtttctc tgcaggagtt agcagcttgc
    24241 ctcccacctc aaggtattta ccccgtcaat gacaatcgat atgattggcc ttactcagaa
    24301 tgactaaaga attagacgat gattcgattc aggaaaaaaa ttctctcata tctaatggca
    24361 gaacattctg cctgagcgat ttcgagggtc ctctcgatct tctgttgtat cttataaaaa
    24421 agaatgagat gaacatcaat gacatccgta tttcgagcat cactgaacaa ttcctacagt
    24481 gcttagacag cgatccatcc gcggatcttg acgagatatc ggaattctat caaatggcag
    24541 ctacgcttct ttatatcaaa tcgcgtggac tcatccctca tattgaagat gaagctgaag
    24601 agctcgatga tccaagaaga aaccttatcg atcaattgat tgaatatcat cgcttcaaga
    24661 aactctctga gctcatggaa cagcgcgaaa tggaggtgga gtggtttgtc gaaagaaagg
    24721 tcgcacagcg cacactccct tttgcagaca atccagatat cgatccttgg atacctgccg
    24781 attcatggga tttgctaaga atcttcgctt ctatgattcg aaactttagt tcagagcgca
    24841 tcatcgatct ttatgaagag gtaagtatca atgagaaaat cgcgcttata tacgaactat
    24901 tgaataaaaa aggcaatttt cgtttcgatg atctaatttc tcgaaatact tctgccatgg
    24961 atctcgcatg tgcgtttctc gctgtgctgg attcgacaaa aaataaattg ataagtgttc
    25021 gtcagcataa gctctttggc gacatactga taattccata ttcgacgacg gagcctcaaa
    25081 atgaacgaac agcttgatct cgatgcagca ttgattagcg caattttatt tctagagaat
    25141 gagcctattt cagaagaaaa acttgcaaaa atctctggct tgacattgga acagacatca
    25201 ctggctcttc agaggatttg ttcggaattc gatcagccga tccatggttt tgccccaatc
    25261 aggagagaag agagatggat ttttgctcct aagctggctt tttgggataa gctaaaagat
    25321 cactatggca aaagaaacga aatgagactc tcgcgtgccg ccatggaaac ccttgcaatt
    25381 atcgcctatt ctcagccaat tactcgagcg gagatagagg caattcgtgg ggttaatgtc
    25441 gacgctatgg ttaggcttct tcttgaaaaa aaccttatag cggaaattag caagcgtgat
    25501 gtgcctggaa agccgatgca atatggtact accaaggaat tcctaagatg ttttggcttg
    25561 aattcgatcg aggatcttcc gaagctcgat gaagtagaaa attctagatt ctcaagactg
    25621 gagacaccag aaggatgagt gagtccatag agaaaccaat gcgcctacat gcttaccttg
    25681 cttctcttgg catagcttct cgacgctctt gcgaggaaat gattagagaa ggaagggtga
    25741 ccgttgacgg tgtaaaggca tccattgggc aaaagatcat ctgcaatcaa agaatagaaa
    25801 tagatggaca gccaatcatt gcagcctatc ccaaaaaatt ccgctacatt cttctaaata
    25861 agccaaaagg ctatctctct tcgctcagcg atccatacgg gcgcccttgt gctatcgatc
    25921 taatatcagg cgaagtcaat gagcgaattt acaacattgg cagactagac caatggtcaa
    25981 gtggtctcct tctatttaca aatgatggag acctggcatc taagctcatg catccctcag
    26041 gccaaatgga aaaagagtat aggatccaga ctaatgtgcc gattccggat aattttgcat
    26101 cgagctttat gcgaggaatt attattgatg gtgtacgata tcgcgcccgt tctgtccata
    26161 tcacaggacc taatagcgct ctgattgtcc taatagaggg aaaaaatcgc gaaattcgcc
    26221 gcgtgttaga gcattttgag cttcatacaa ggctgctgga gcgcattcga tatggtccca
    26281 tctgcgatcc aggcctagca ccaggaaact ggagagagct cacagtagat gaagtacaag
    26341 cactcaagac tctttgtaat ccgaatcaaa aagccaaagg gaagggacaa tgagagtggc
    26401 aatagatggc ccggcaggtt caggcaaatc cacaattgcg aaaatgatcg cagaaagact
    26461 tggtttttta tatataaatt caggcagcct ttatcgcgca attgcatttc ttgcccttcg
    26521 tgaaggtgtt gcatggcaag atgtagaaaa aatgataaac ctcgctaaaa agcatcgctt
    26581 tgattgtctg tctgatgggt cggtcattgt agatgaagag tgtcttaagg aagagcttcg
    26641 gacaccagag gtagatgata tcgtctctca agtctctgca ataccagaaa ttcgcgctga
    26701 agtgaataac atcattagaa gaatatccga aggtaaggat gttgttagcg agggtaggga
    26761 cattacaaca gtcgtctttc ccgatgccga tgtaaagcta tatctggatg cctctcctaa
    26821 aattcgggca gaaagacgat atcgcgaaag acttagcaag aaagcttcga ccaaggagga
    26881 aggagccgaa gccgattcga tttcaattga agaagtacgc aagaatatcg aaatgcgaga
    26941 taagctcgat gctcaaaaac ccatcggtgc tcttaaaata gcggaagatg ctatatattt
    27001 ggatacctct gacttgacca tagagcaagt ttatgacaaa gtatacaaaa ttctaagggc
    27061 aaggattgca catggcagat aatatggaag tgggaatgga cgtccgagat ccctccaagg
    27121 acgatatcca aacacaattg caggagcagt acctcaaatc atttgagggc ctggaggagg
    27181 gagatttagt cgacggaaag gtaatccaaa ttgctggcga cacggttttt atagatgttg
    27241 ggtataaatc agaaggaaaa ctaaatatat tggagtttgg cgataccccg cccaaacccg
    27301 gagatgttgt caaggtaatt ttgattcgca aagagacaca ttcaggcgat ataataattt
    27361 ctaagaagaa agccgatgaa aaaattttct ggaaatcgat agccaatgca ttcaaagagc
    27421 atcagcctgt ggaaggcacg atcacgaaag aaataaaggg cggattcgaa gtcgatcttg
    27481 gtcatggatt atcaggtttt ctgcctttaa gtaaggcaga tgttcaacgt gtagaaaaaa
    27541 gtgaacagct tattggaatg cgatgcaggt tctatcttga acgcctgtat tcagataaga
    27601 agataaacat cattctaaac agacgaaaac tgatggagga agagatagag cgcaaacgtg
    27661 agtccttctt ccaggaaacg cagattggcg actctgtcag aggtgtggta aagagtttta
    27721 caagctttgg tgccttcata gacctgggcg gattcgacgg ccttctgcat atcaatgaca
    27781 tgagctgggg tcatgtgact cgccccaaag attttgtacg aaaaggccag gaaatcgaac
    27841 ttaaggtgat tcgtctagat ccggaagaaa agcgcataaa tctttcgcta aaacatttta
    27901 ctccggatcc atggaacagc tttgaagata aatatcatgt aggtgatatc gtaaagggaa
    27961 aagtcactaa gctcactgat tatggtgcct ttatcgagct tgaagagggt atagagggtc
    28021 ttgctcatat ctccgaattt tcatggttga aaaaagtgcg aagacctgag gatgtgctaa
    28081 aagctggcga tgttgtcgac tgcatgattc ttggatatga catcccggca ggcaaagtct
    28141 cgctaggtct caaacaggta caaccgaatc catgggacac aatcgatcaa aggtatcccg
    28201 taggtacacg gctcaagaga aaagtggtaa aggttacaaa tgccggagcg tttatagaat
    28261 tagaagaagg gatcgatggt tttcttcata tagatgatta ttcctggacg caaaaagtaa
    28321 agaatccatc ttctgtgctg catgagggtg atgaaataga agtcattgta cttgagagca
    28381 gtcctgagga aagaaatatc cgcttaggtg ctaaacagct tacggaagac ccctggcgtt
    28441 ctttcgccaa gacatataag ccaggctcaa ttgtagaggg aacggtatct tctgtaactg
    28501 acttcggtgt atttgtaaag gtgccgggca atatcgaagg tttgattaag aagcaggatt
    28561 tggtatccga cagaaatgaa tcttatgatg aagcccttaa gaaatatcat cctgaaatgc
    28621 ccatcaaggc agttgtaatt gatctcaatc cggaccgcca gaaactgggg ctttccataa
    28681 gggacttggt ccaaaaacag gagcgcgagg agatttcccg ctttattcag gaagatgaag
    28741 gagaggcagg ctatacgctg ggtgacctct tgaaggcaaa ggataacgag aacggctgag
    28801 cacttttgtt cagtcttctg caatggtaga aatggcttct gaaatagatt cggatagatt
    28861 ccgtacgttg atcgatatca acgcgcaact taattccagc tacacaaatt atcgctcttt
    28921 gctgaagact atcgtggaat cggcagcgag acttgtcgga gccgaagcgg caagccttgc
    28981 tctgttcgat gcctccacag aaagcctgag attcgaaatc gcgcttggtc caagaggtac
    29041 cgagctccag gggaagtcgg ttcccgcagc agaggggatt gtgggctggg tattcaaaaa
    29101 tcgccgctct atcattgtaa atgatgcagc taatgaccct cgtttttcac ccaaggtatc
    29161 aagagaaata tcctatagca ctaattctat tctcgcagtt cccctcaaga taaaggataa
    29221 aacagaaggc gtaatagagc tgatcaacaa ggcctctcat ggcattttct ccagcgaaga
    29281 tcttaagtgg gtagagcttt ttgcggtaca agctggaatt gcctttgata atgcaagaca
    29341 gtatgagaaa acagaacaag agcttatcta ccttcagcat aaggttcagc aaggagaggg
    29401 atggcatcct cttatttttt cttctggggt catgcgtgat cgacttgagc tggtaaagcg
    29461 aattgctccg agcgacgcct cggttttgat tcttggagag agcggagttg gaaaagaact
    29521 tatcgccgag cagttgcatc ttctttcaaa acgaagcgat ggacctttta tgcgtgtcaa
    29581 ttgcgcagca ttgcccgaaa gtcttttaga gagtgagctt tttggccatg taaaaggagc
    29641 ctttactgac gctatagaaa atagaactgg aagatttgaa gctgctaatg gcggaacaat
    29701 tttcctcgat gagattgcag aactccctct gaagcttcaa tcaaagcttc tccgagtcat
    29761 tcaacaaaag acatttgaga gaataggctc aaatgagact ataaaagtgg atgtgcggat
    29821 aattgctgca acgaatagag atatagaaaa actagtaaag aatcaggaat ttcgaagcga
    29881 tctttattat cgcttgaatg tactgcctat acaaatacca cctctgagag agcgtctgga
    29941 tgatgtgcca actctagcgg agtatttcct taaaaagttc acaagggaaa caaacaaggg
    30001 aatcatgcgt ttttctgaat cggcaatgca aagcatgctc agctacggtt ggcccgggaa
    30061 tattcgggag ctggaaaatg cggtagaacg cgctgtcctt ctcgcaaaag gacaggtgat
    30121 cacggccgat gacctgatga taggctctaa gtcgagcatt gaggatgaac aatttggcgg
    30181 aaaagatcta agagaggcaa ttaccatctt taagaagaat tatatagaat ccgctctaga
    30241 gaggaatcgc tggaatcaga cagagactgc tcgaaattta agaattcaga ggacctactt
    30301 gtcacgattg atcaaagagc tcaatattct gcaaaacaag gagtaatttg tgaatcaaga
    30361 agaaatgcaa caagaacagc acaataagga agagaagaaa agctcttcag agagattcgt
    30421 cgactttata agcaagaata gaagaattat aatcggcctc ggcattgcaa tcgtactaat
    30481 catcgttgct ataggaattt atactgcagt ctctggaaat atcgcttctg catccagcag
    30541 agcgatggaa ttggccgacc aaaaagcgct acagtggtcg caagagaccg atgaagaaaa
    30601 aagagccgaa attgagagtt ccctgatagc cgaactggac tccatagccc aaaaatggcc
    30661 aaggacactt gcggctcagc gagccctgct tagaaaagcc gcgctcctaa gtcaaaaaaa
    30721 agaatatgca gaagccgaaa agactgctct tgaggcttat aggagaaata aaaaatccta
    30781 tgtagcgccg attgctctcg agctcgccgc tgttgcagca gaagaagcgg gtaacaccga
    30841 tgctgccctc gaacactaca cattgattac aaaggattac aagaaggata atcctgcagc
    30901 ggctcacgca ttcttcaatt taggccgcat aaaggaagcc aaaggcgatt ataagggcgc
    30961 tatcgaagcc tataatcaac tcatagcatc atttggagat tccgaatggg caatgttggc
    31021 caaaaatcgc gtgatatact tgaatgcgca gggactagct ggttgagtga attctcaatt
    31081 atggcctgat tttggtaaag gcttgggttt gattttgtga atggcttgat ttttggtaaa
    31141 gaaggaagct agaagagatg aaaggggagg ggaaagagaa aggaataaga ttcttgcttc
    31201 agcccatagc cgcacttctt attataaata tatatattat aatgacatcg tgtggccttc
    31261 ctacaacaga ttatttgtat actccgaaag atttttcttc agaaggtgga gcactggttc
    31321 ttgaacatga tacaaagaat atcgatgagc tatcttccat atttctagga tatgaaatat
    31381 attatcgcat ttttgatgat gaagtagcag caggagatag ccgcaattca atcagcagca
    31441 tgaagagctc caatataatt cagacagcca ataattataa atatttccca ttgaagcata
    31501 gaaaaaaatc tgaatctggt caattttctc ttgatattag tccattaatt aaggtgtcta
    31561 gcaatactta tacaaaatat taccttaata tgaaaccgca agaaacatgg acgcttacgt
    31621 atgaagactc agaaattgtg attgattcta ttgttcgaaa cagaagtgat actgcgagta
    31681 gtgctgaaat tgcagatgct gatttttgtt tgtcttcagg atatcaggtc aatgatcgag
    31741 attatgctgg ttcatcttct tcaccggaca tagtctatat agtgttcttt gccttcgctt
    31801 atggctttga cacttcgact atgagaaatg tctatagcga tccttttatt ct
//
LOCUS       DAOMZL010000011        31057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_1127_length_31057_cov_17.263789, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000011 DAOMZL010000000
VERSION     DAOMZL010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 31057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31057
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1127_length_31057_cov_17.263789"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLQ30_01885"
     CDS             <1..359
                     /locus_tag="PLQ30_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPW01223.1"
                     /translation="GYVSRGRGIIVHREDCRSIASIADFEERRIDVKWEDEAINTTAR
                     YRVTTKKSYDIFSEIESTVRRCSGRLKEGKLGERGDGTLSGFFILEVDSREGLKRVAK
                     ALRTLPSILSIEEEPI"
     gene            complement(369..1718)
                     /locus_tag="PLQ30_01890"
     CDS             complement(369..1718)
                     /locus_tag="PLQ30_01890"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPW01224.1"
                     /translation="MTVCLNPIEYEEDISIILAYRAGANSQTASTAGLFKLLEYVLFN
                     GPAANPGIPEPAAAIDVLEAEDIRGGLGIDRFEFGISFKKENLVQAFDTLLYLFSQER
                     REFIFAQKDGIELARRSILNLIQNETSDNDLVSSMAINKKLFAKAPYRIDILGSENVL
                     EKADAAQLKNIASSWLVPNNACLSIAGAFDPNQVLSLIEARFGLLQKAKNPWPANLAP
                     FPRPGVTKPIFLVFPDSDISADHMKIEMRYRGPDPKDTTSYFAALMLQQLANEPTSRF
                     QMAVRKSMPKGSEPENIRIDYVPNTDSSWISVLSIVPAGKKPAETVFAFKEAVRGTEL
                     YMVKANPSYFSAADYDRACQALLEEIMAIQGDAMSSACHMATLWSWGIPSLIFQESDF
                     IMKTRQKNISQFVDTYIQKNLEVVMVRLNPDLYEVNKKSFSSYGFETVSTKNAFWWR"
     gene            complement(1855..2214)
                     /locus_tag="PLQ30_01895"
     CDS             complement(1855..2214)
                     /locus_tag="PLQ30_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW01225.1"
                     /translation="MDYVRYCFASNLRLRRAILQMSQEELAHLANLSPGFIANIETGR
                     NFPSSKGILKISSALKVEPWKLFLDPQKQDMFFTRDEVSHWLEDSRKRLFGDFSQGEY
                     HTEDDSEIDSNEITPQD"
     gene            2368..3786
                     /locus_tag="PLQ30_01900"
     CDS             2368..3786
                     /locus_tag="PLQ30_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HPW01226.1"
                     /translation="MFIREIYIKRRRAFLDAIRDRGITSGKVLLLANENCPRNYPENY
                     YPFRQDSSWLYLIGLNIPSLACTLDIESGENCLYADEPGLEEIIWTGVLPSPSELAYS
                     AGIDKVLPTSVLSNTVGRALKQREPVFILPPYRDRQIARFLDLFAVSVSNLEELIYRH
                     IDPRIILALVSVREIKEPEELSELERAVSITKIIHEELMHELKAGWTEKDAAGFVSSR
                     ASAQGCELSFATIATCRASVLHNSPTEHVTTEHDTFLLDAGVETTLGYAGDLTTTFPV
                     GPSFSHQSREIYQVLYETLQTATNALAPGVRFIDVHKIACLTITKGLLSLGLMKGDPV
                     EAVESGAHALFFPHGLGHQIGLDVHDMEGLGEDYVGYGDELKRSEQFGLSSLRLAKTL
                     KPGIVHSVEPGIYFIPQLIQKWRAERICESFLNYDMIEKWMPIGGMRVEEDWCITDKG
                     ARRLGPAFDRSIEAIQKVRANR"
     gene            3783..4010
                     /locus_tag="PLQ30_01905"
     CDS             3783..4010
                     /locus_tag="PLQ30_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01227.1"
                     /translation="MKTVFVSAFLEDSIVVKTMLESAGIPAEVLSDQMLDINPFYSIE
                     PKGAKVIVPDSSEEDAKAIVADYREHKQSNK"
     gene            4050..5264
                     /locus_tag="PLQ30_01910"
     CDS             4050..5264
                     /locus_tag="PLQ30_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPW01228.1"
                     /translation="MKRKLFVPITITLASLLLIGLLIYSIQRHIAGRNFESVNDALSS
                     IIEIQAYAFYNGCLSNNQTYNLVIQGEFAEANALVQKATDSFSLIDLAEIVEGKPPSD
                     SFDIIMDGELLRIECPIRNDDKTAIVPNMIGVAIIQAQRLLNTVSAKDFRIDLAHGRK
                     TTYGIPISANFTGNRLWDSIIIALLTGFVAIFIFQHFYRNANTFLDTRGLESIIYLFE
                     QTERISASHSRNVAMLALFVGKNLGFRGKRLKDLYIAALLHDIGKIGIPNNILTKNGK
                     LDPCEFVQMRQHPAISARILRSFKEFERLSPIVLYHHEREDGSGYPEGLTGRDIPFES
                     KIIAVVDVFEALIGERPYRDPICPLSAFQKMRSMPLDQKIVSLLMDHYGELDGFRPPR
                     WALSYSPWMLTS"
     gene            complement(5259..6356)
                     /locus_tag="PLQ30_01915"
     CDS             complement(5259..6356)
                     /locus_tag="PLQ30_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPW01229.1"
                     /translation="MTEIYSRLEEAHLLFFEYGITALDAYLDEKPRPLKFLAVQCSLV
                     ELARVFDVLEYPNLPYADASLCDEVRFICTEDALEENLGSPWMNFKRSPSTGVYYDKG
                     DIYNSLKARNIQPSSSEYSEIERSLFDFTLLATLSELPPKTIGSIALPPSPSVLFQRD
                     LLIGILSGPYPVSGFEVLKQSGFIAREWPEIALLDNVDHSKDFHPEGNGWNHTMQTFS
                     YRKTRDITLSLALLLHDIGKSESKSQKGHRFDRHAELGAKRAEKFLSRLGFSEEIQED
                     VKFLIRYHMMPAALPILPVQKTEALIRDPRFSVLLELFRCDEFSSFKNAERYYDACNM
                     YQSIRRNLRNPYRKADGSKIHASKRSYSHFS"
     gene            complement(6349..6804)
                     /locus_tag="PLQ30_01920"
     CDS             complement(6349..6804)
                     /locus_tag="PLQ30_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01230.1"
                     /translation="MMQTDVDAFWANFEQEIGDKIISKTMGQHFSSKKSQGEWGLLVL
                     TASSIRFRPTPGENWFQSLFRMAAPKVPKETASDIVLPLTLITSIELPKRHFFDFLFS
                     PPFTVFALTYRLGENEEHKILLGADPKSELFMKLISTIPVMQGKCKEND"
     gene            6956..7894
                     /locus_tag="PLQ30_01925"
     CDS             6956..7894
                     /locus_tag="PLQ30_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPW01231.1"
                     /translation="MRRCYFSIMNRMGGILAGLGYSTIFGFSFLMTKEALEVLNPMEL
                     LAMRFIIAALLMSILVIAGIIQVDFKGKPIGLLALMCLFQPIAYFTFETYGLANAATN
                     VAGIILGALPAGVAIMEALLLKERLSALQSAGLSMSIVGVILVAVFGQGHNTETKPIG
                     IIFLTGSMLSAVFFNIASKKAAESFSPVERTFAMMWSAAICFFIPALFENIKGKGNIS
                     HLFGEWNRIAGVWGGILYLGILSSVGAFFLINYSLSQLKASQSAVFTNLVTVITVLAG
                     VLIRKEQFSWSQGLSVILIILGIAVVNSSFKEKAKS"
     gene            7920..9185
                     /locus_tag="PLQ30_01930"
     CDS             7920..9185
                     /locus_tag="PLQ30_01930"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPW01232.1"
                     /translation="MQDAAFYDIHCHLLNLSHPAFVSIIESMRHRPREVIYSQIASFD
                     YLASSLLKRGGENLRNLLAVMDNDCADILFLLEDDLSGQFKEPKKTPGFRQAPPLRNG
                     KLYIADQQFSSFVLTPLIMDFLAPSSFSPETYYNRPPQKWIEAQIIDVLFAICTYREK
                     RPSGFLKIYPFLGINTGHYTAEELSAFLEQWLGNYTKSENDIERRFLSYSFDAIADFQ
                     SYSIFSGIKLYPPMGFDPWPEDPAEREKVELLYSFAETKGVPITTHCDDQGYRIISLE
                     ESLKFTAPSRYRPVLQKYPRLILNFAHMGKRHIRTIRGNEQADWRKDIFDLIIEYPNV
                     YSDFSFTAGEPSFYEELASTLNHLKEPEQEKIIQRILFGSDFMVNLFKVRSYRDYLEY
                     FSESVLSVEKKRLFCSDNPRRFLFGDQKA"
     assembly_gap    9214..9313
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9384..10136)
                     /locus_tag="PLQ30_01935"
     CDS             complement(9384..10136)
                     /locus_tag="PLQ30_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HPW01233.1"
                     /translation="MTEPFEETSPFKKHEGSFLDRTKIIAGDDGLARIADSKVAVYGL
                     GGVGAACALDLVRAGVGYLHVVDYDIVEYSNLNRLAFGYNRYVGLAKTEAFSQAAKEI
                     NPAIQIMSVRSFFSAESASQIIARECDFHADCIDSIMPKVILIASLYRERSAFISSMG
                     TAGRIRPESIKLGKMQEVKGCALARAVRLRLGKMEIPLDFPVVWSDEPPTKPIVQETK
                     RIIQGSMPFVPQTAGHMMASWIIRRILQKDAP"
     gene            complement(10247..11026)
                     /locus_tag="PLQ30_01940"
     CDS             complement(10247..11026)
                     /locus_tag="PLQ30_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPW01234.1"
                     /translation="MHNIVMYCDAHLHLVGLEQYEPDFALRALPFDWRGAVVSHNPEE
                     FLQSEALRANLPSTIAGFGIHPQEPDMRHADFLSMLCEQRKVDFIGEAGFDFFGDGPL
                     RSRSSEAMHLQLEAFLFQVHLSAYTQLPLVIHIRKATDVIMAYGRELRRLPSIIFHCW
                     PGRLEEAQMILKKGINAYFSFGTPLLRDSRHAIESLVGLPTSRILSETDAPWQPPHGA
                     EYTSLEHITNVVEKMSSVLNMSSEELRHELSVNFSAAYLHC"
     gene            complement(11145..12848)
                     /locus_tag="PLQ30_01945"
     CDS             complement(11145..12848)
                     /locus_tag="PLQ30_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPW01235.1"
                     /translation="MRETEPWKFLDAYRGRIFTSKWPTIPQLFDITTDRFADRACFTI
                     YSPDRVSLNYRETREIILRVASYLTSIGLGPGSHIAVTGKNSPEWGIAYIAVLYTGAV
                     VVPIDYQLSTQDIITLMHAGDVTAAFVDEEKYEHIKKDFAGIPCFGLARGVESFIYDL
                     EPPAGFVFTPPQVPVQSTAAILFTSGTTGDPKGVMLSHENLVSDCFLAQSNLNISEQD
                     IFYALLPIHHSYTMLAVFIESFSVGAETVFGKRMVVKQILSDLKEAKITMFLGVPMLF
                     NKLLTGILKGVKEKGALAYGLIRTLMAISGFIKKTFGVNPGRAFFGSILEKASLSSIR
                     ICISGGGPLDPKVFRQYNQLGIDFVQGYGLTETSPILTLNPVEHYKETSVGKVIPEVE
                     MIIDNPNEDGIGEIVVRGPMVMQGYYKMPEETAEVLSPDGWLRTGDVGKLDDENYLYL
                     AGRIKNVIVTEGGKNVYPEEIENEFQLFDEIDQILVRGYIPEGKAVAEEIESLIYPNQ
                     DWVSQNNLSQEQVETRIHDIVNQVNQRLRSYEKITRIHILKAPLEMTTTKKLKRHSAK
                     E"
     gene            12999..15656
                     /locus_tag="PLQ30_01950"
     CDS             12999..15656
                     /locus_tag="PLQ30_01950"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPW01236.1"
                     /translation="MKSVELAKAYDPKSFEERIYEMWKREGLFKPKIDKNKRPFTIVI
                     PPPNVTGALHLGHALDNCLQDIQVRYRRMKGIPTLWLPGTDHAGIATQNVVERKLRKE
                     GRDRESMGREAFIEETWKVAREHRAFIDNQLARIGSSLDWSRERFTLDEGLSKAVREV
                     FVALYERNLIYRGEYLVNWCPSCGTALSDDEVEHEDEPGSLWYIWYELVDGPCRECPS
                     GRIEIATSRPETLLGDAAVAAHPDDNRYRAIIGKKVHLPLTDRIIPIIEDSYVDPEFG
                     TGLVKITPAHDPNDFLVGQRHGLENINILNPDATLSDAVPEKYRGLSVLDARKAVLAD
                     LKAQGLLKNEEKIVHAIGHCYRCHSAIEPYLSKQWFVRMQPLADKALKAWQDGDVVFF
                     PKKWEHTYRHWLENIRDWCISRQLWWGHRIPVFYCIDCGEMMVCREDPSVCIHCGSSN
                     IYQDEDVLDTWFSSWLWPFSTLGWPEDTEDLRYFYPTSALVTGYDIIFFWVARMIMAG
                     MEFTGQSPFKEVYIHGLIRDKQGRKMSKSLGNGIDPLDIVEEYGADALKFTLAYNCAA
                     GQDILLDKESFKLGSKFANKVWNASRYILMNLENRAFLEDDALTYSDIDWWILHKLNE
                     AAKAAIQAFESWRFNDAAQAVYSYFWNDFCDWYIEASKLSTKSGSESEKDRSTTMLLR
                     VLEESLRLLHPFLPFVTEEIYGMLPNAKGRLIIASYPVWTKDRESPEIESQFKALQEI
                     VTLVRTLRSEFQIAPEISIPLSLEFDNHFSHGQFIQSHIELISLLAGASSVSISKATE
                     KGSVSLVGNGFTVHVGVGGLLDIARLINRLSKEAERERAYIEKLRAKLGNRTFHSSAP
                     SDIIEKEKEKLRLSMAKAAKLEQYIEELS"
     gene            15734..16288
                     /locus_tag="PLQ30_01955"
     CDS             15734..16288
                     /locus_tag="PLQ30_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01237.1"
                     /translation="MNKTAMLRLKRMHLAPYIQLATSLIGKPRRSGGNMFRHQLDTMA
                     ILIDYGYIDSVLLKASLIHDLLEDAPETNPDSILSIDDESIDVYKLVLEVTRRPVENK
                     AEFLIRIRDYGTPHARVLKSADRISNMISLGYVTDDVFVRRYTDETEQYIYPIAERAD
                     ERMLMELRELVATRREYLRNRIEI"
     gene            16325..17026
                     /gene="deoC"
                     /locus_tag="PLQ30_01960"
     CDS             16325..17026
                     /gene="deoC"
                     /locus_tag="PLQ30_01960"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPW01238.1"
                     /translation="MKKKWTSLMLAKVIDHTLLKATATEDQIKALCVEAKEFGFKAVC
                     VNPYWVPLCVRELADSDVLVATVVGFPLGANASLIKAEEARLAVAQGADEIDMVINIG
                     KAKAGDWAAVRDDIAGVVQASRPAIVKAIIETCYLTQEEKIAACEAAISAGATFVKTS
                     TGFGTGGATVEDVSLMKRTIGEVAHVKASGGIKTCEDVVSMLEAGASRIGASAGVAIM
                     RESEALDNKSPEQLS"
     gene            17148..18152
                     /locus_tag="PLQ30_01965"
     CDS             17148..18152
                     /locus_tag="PLQ30_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPW01239.1"
                     /translation="MKKALLVLVVTALIAVPAFAQFKVGLVTDVGGIDDRSFNQGTWE
                     GIVRFAQDYKLPKSNYKYLQSSAEADYVPNLSTFADEGLNLIIAPGFLFEQAMTQVAK
                     NYPTRKFLIIDTVVSLPNVVSATFAEHEGSFLVGVAAALKAKADGKNILGFLGGMQFP
                     LIEKFQAGFEQGVKAVYPDCKILVDYAGDFAAPDKGQALAQKQYNAGAYIIYHAAGGT
                     GNGLIKEAKERQQKGDLRWAIGVDKDQYADGIYSGNKSAVLTSMMKRVDVAAYEVSKM
                     TMEGKFPGGQVLVFSLKNKGVGIPDNNPNLSADIVAKVKEYEALIASGKLVVSEIPAK
                     "
     gene            18431..19987
                     /locus_tag="PLQ30_01970"
     CDS             18431..19987
                     /locus_tag="PLQ30_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW01240.1"
                     /translation="MPNIVEMLHITKDFPGIRANDDITLQVEERSIHALLGENGAGKS
                     TLMSILFGLYQPDAGTIRIRGKEVKVSDPNVATALGIGMVHQHFKLVHNFTVTENIIL
                     GMEPRKGILVDMHKAEQKVEEISKLYGLAVDPKAKIDEISVSMQQRVEILKMLYRDAE
                     ILIFDEPTAVLTPQEIRELMQIMRRLVEEGKTILLITHKLREIKEVADVCSILRRGKL
                     VGTVPVADTDEERMAEMMVGRQVSFHVQKTRAKPGDVLLRVEGLNVKNQKGVLGVRNL
                     SLSVRAGEILGLCGIDGNGQTELVHAITGLTKVESGHIFLRDIDITHLSINERSDLGL
                     GHIPEDRQKHGLILDFTLGENFVIHNYYKEPFAHWGILNKKAILDNAQRLIREFDVRS
                     GQGPLTPARAMSGGNQQKAIVAREIDRSPDVFIVVQPTRGLDVGAIEYIHKRIIGERD
                     KNKAILLVSLELDEIMDLSDRIAVIYNGEIVGIVDAATTNENELGLMMSGALHKAHEA
                     TQTGREPAHV"
     gene            20006..21100
                     /locus_tag="PLQ30_01975"
     CDS             20006..21100
                     /locus_tag="PLQ30_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW01241.1"
                     /translation="MPARKEKIVLPLIAVLLGFLLGSLIVILTGRSPVSMYAAIIKGF
                     SGIDIINGQPINTRYIGEFIIQAMPIILTGLSFAFASRTGLFSIGAEGQLMMGSISAT
                     AVAILVQAPKVIHLPMVLLAAILGGALWGAIPGFLKARYNVHEVVVTIMMNYIALHFN
                     NYMILNIFGSVDKVKTVPFPDSALLKSPFLESITRNSRLNWGFIPVLLAVLIFSFIIN
                     KTTFGFSLRAVGHNKEAARYAGMKVNRNIIASMAIAGAFAGLAGGIITVGTFNFGRVI
                     QAAEGYGFDGIAVALVGGNEALGILLAGLLFGGLKAAQPLMQSQGIPREIAGIIQASI
                     VLFVAMKFGIKMVLDKFERKQVPTTEGERV"
     gene            21097..22050
                     /locus_tag="PLQ30_01980"
     CDS             21097..22050
                     /locus_tag="PLQ30_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW01242.1"
                     /translation="MTFEVFLQMFPIALMFSAPIILTALGGLFSERSGIVNIALEGIM
                     MVGAFTGATITVLLENSTPAAAWIGLLGGLLSGVAFSLLHAVASINLKADQVISGTAL
                     NILAGGLTVYLCQIIFHQQRTRAFSYGIKKLSVPGLSKIPVLGRMFFDQVYPTFYIAI
                     AIVLLTWFVVYKTPLGLRMRACGEHPQAAASMGINVAKMRYFGVLISGALGGLGGAVM
                     VLTADIQYTLVSIHGTGFISLASLVFGRWNPFGVLGAGFFFGFSTALSFYAKDIPLLS
                     GLPGEFFYILPYLLTIIALLFFAGKSVGPKAAGQIYESGER"
     gene            22082..22519
                     /locus_tag="PLQ30_01985"
     CDS             22082..22519
                     /locus_tag="PLQ30_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPW01243.1"
                     /translation="MIEVDQLQKYSLFGGISPEQIERIKPLLGTEHFEAGECPQVEGQ
                     PNDKIHFILSGEVEIIKKGLVIARLREGQTFGEMELLDIMPSIATVRAATALETVTIS
                     NRALYDIFKLDLRAFSMMIMNLARDLSRRLRRMDDIASSQGKC"
     gene            complement(22667..22752)
                     /locus_tag="PLQ30_01990"
     tRNA            complement(22667..22752)
                     /locus_tag="PLQ30_01990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22716..22718),aa:Leu,seq:taa)
     gene            complement(22888..23706)
                     /locus_tag="PLQ30_01995"
     CDS             complement(22888..23706)
                     /locus_tag="PLQ30_01995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPW01244.1"
                     /translation="MTASKNLDPSKLRFIAMDMDGTILDEGYQLSPHVADTLKKLQNQ
                     GKKLIIATGRIYKAAKIVLADSFEPDAFVCTNGADIYNRDGTILASHHIPTEALPILT
                     KVSRDHDVLFCCYVGERWIYDRYSPGVDFYEKRTGMKGVRKNFDSLVGEDILKFLAVG
                     PNEKLLEMRETILNSAPDLLDAFFSHETMLEIVASGVSKRQGLEECLRYLGGSLEETI
                     AFGDAENDLEMLRSVKVGVAMGNAHSYIQTAVPYVTASVDDDGVARFLEELFGI"
     gene            23927..25201
                     /locus_tag="PLQ30_02000"
     CDS             23927..25201
                     /locus_tag="PLQ30_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPW01245.1"
                     /translation="MTYNHYDDTYILEGADLVLPDSILPKATLAVRGGKIAAIIPSGQ
                     FQRPEKLNRSSQSQIDPAFENAPVIKAEDAFVLPSLAEMHIHGCASWGFERISEPGDL
                     FQIAALLEAKGVGCYVPTILWEERTLIELTETILASGISAPLLPGIYLEGPFVNHSKR
                     GGIMPDNIRAPDIALAEHILDISKGLLKICTMAPELDGIDQIYPVFQNKAVLVSLGHS
                     DAKLSSIHPPSRPFSITHLFNAMTGIDHREGGLANFAFSQSPDYIELNGDGIHVNESC
                     LKLCSRLIPENSLILISDAVIGAGLPYGEFSYYGRRVVSSERGVRYADSGVLMGSNML
                     GIDIVKNYYLKAEVPLWRAVRAMSLTPRKALGMDGEYGSIEVGKIADIFIWDKGLETA
                     VRPKALLASENDYKPNDLLSSKFKVHRVQKKD"
     gene            25205..26329
                     /gene="alr"
                     /locus_tag="PLQ30_02005"
     CDS             25205..26329
                     /gene="alr"
                     /locus_tag="PLQ30_02005"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPW01246.1"
                     /translation="MDHRATTAWIDLAAIRANFRHLRDIAQGSIVCPVIKADAYGHGA
                     LAIAHELEREGAQFCSVALAKEALDLRESGIQMPLLIFGAPEHSSSSLAIRLECAQTI
                     YTEEHVRILASAAKSVGKKAKAHIKIDTGMNRQGLPWTKARSFAGLLADYPEIFVEGI
                     YSHFADADSPDPSFTDLQMKRFSIALESFERVGITPKYRHIANSAGMVLYPKTRLDMV
                     RPGILLYGLRPAPNIELPEGFEPALALTTGIVHIREVPAEESISYGRTFTTKRFSRIG
                     LLPIGYADGYPRALSNRASVLIRGVRAPVVGRVCMDMTMIDLTGIAGARVGDEVLLFG
                     GRTLPVSEISNLLSTIDYEVVCMISKRVPRVHIDSKPQEE"
     gene            26332..27255
                     /gene="dapF"
                     /locus_tag="PLQ30_02010"
     CDS             26332..27255
                     /gene="dapF"
                     /locus_tag="PLQ30_02010"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPW01247.1"
                     /translation="MKILQQGFHAHKYQALGNDYLVIDPAEFQMSFSASAISALCDRN
                     RGVGSDGILYGPILSQTSLVICSPFGLRIFNPDGSEAEKSGNGLRIFSLYLYERGFVG
                     KEPFEVQTKGGMVECRILATNSPCSGEDGYRKDDHREEGYEEEHYVEVSMGEARFLPG
                     SSTLAIDGIEFRTIRVSMGNPHCVLLGQYPTEELARRIGPLVENHPDFPNRTNVQFLE
                     VIDRQTIRIEIWERGAGYTLASGSSSCAAAAAARRLGLVENEVRVRMAGGTLYIDMRE
                     PNIKMTGPAEHSFDLVFSPSLMRLAMKDKGD"
     gene            27257..28480
                     /locus_tag="PLQ30_02015"
     CDS             27257..28480
                     /locus_tag="PLQ30_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPW01248.1"
                     /translation="MAQWKNLAFLQKEVFFESRAMEEWLIEMRRSIHRIPEPGNKEQK
                     TSSFICKMLEEMDIPHRSYGTAVVGLIEGKEPGRCVALRADMDALPLEEPSDRPYASL
                     HHGYMHACGHDAHMTVALGVARLLAAHRRDFSGSVKFLFQPAEETTGGAKAMIEAGCL
                     ENPHVDLVLGLHVVPELMAGMVEVKIGPFYGTSDSLEIIVKGRSSHAAYPEQGVDAIV
                     VSASIIQALQTIVSRSISALDNAVLTIGKIQGGVRSNILANEVIMIGTIRALSEDVQC
                     FMRKRVKEICTEIAAAFGASCEVHIRPSYPVMINDQSATELVRDTAISILGESNVIMR
                     QQPNLGVEDFAYFLRERPGTFWHLGCGKPGMEKIHPLHSAEFDIDESCLPIGVAIQAA
                     SALTFLHLRNQESCG"
     assembly_gap    28498..28597
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(28633..29370)
                     /locus_tag="PLQ30_02020"
     CDS             complement(28633..29370)
                     /locus_tag="PLQ30_02020"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPW01249.1"
                     /translation="MKKPLIGITSSVDRSRQPVAYFSLKESYVKAIEMAGGTPLILAV
                     TSSAVMKALTSILDGLIFSGGGDIAPWLFDQEPILGLGSFDTLRDEWEIELCNQAWKA
                     RIPMLGICRGCQLMNVARGGSLLQDIERSNPAALLHNPQIAYDELFHHIAIEKESVLF
                     ELFKTERLLVNSFHHQAVDKTASDFRITARSSDGIIEALEPIDPERFALALQFHPEGL
                     FLRYGAFLAPFSALIEATKGSLSATRR"
     gene            29468..30865
                     /gene="lysA"
                     /locus_tag="PLQ30_02025"
     CDS             29468..30865
                     /gene="lysA"
                     /locus_tag="PLQ30_02025"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPW01250.1"
                     /translation="MKDTPFSPAILIDGSKCYFDGVDLRYLAHEFGTPLYVMSETIIR
                     ERCEELRSAFLCRYPGTRAYYASKALQTLDVLKIIKEEGLGVDVVSGGELHAALNAGV
                     PSSDIIFHGNAKTDEELHYAISSKVGLIAVDNIEEIFRIEEEADRLSRDQRVILRVSP
                     GVDSHTHRFISTGSLDSKFGLPIEHSEVERYVKALALCPHVEFSGLHFHVGSQLLENT
                     SHLAALEIVLNFAAKLSKEFGLQVKEINFGGGFGIRYLPSDRAPALLDFIDPMMSMVK
                     EWSRKENLPMPVCAIEPGRWIVGEAGITLYKVIAVKEIPGVRTYVSVDGGMGDNIRPA
                     LYDARYHVETVSQACAMVPCEKEKNCSKVEVLRERDAEKALGMDSNREPILRKVTIAG
                     RYCESGDIIAEDVFLPDPKPLDILAVYSTGAYCFSMSSNYNRVPRPAMVITKSGDYRL
                     SVRRETYDDLLRREI"
BASE COUNT         8358 a         6755 c         7438 g         8306 t
ORIGIN      
        1 taggctatgt ttcgcgtggt cgtggaatta tcgtacatcg cgaagactgc agatccatag
       61 cttcgattgc ggatttcgaa gagcggcgca tcgatgtcaa atgggaagac gaagcgatca
      121 atactacggc acgctaccgt gtaactacta aaaagagcta cgatatcttc tcagagatag
      181 aatccaccgt ccgcagatgt tcaggacggc ttaaggaagg caagctggga gagcgtggcg
      241 atgggacact ctcaggtttt tttattctgg aagtcgattc acgcgaaggc ctaaagcgcg
      301 ttgccaaggc tttacgcaca ttaccgagca ttctctctat cgaagaagag ccaatatagg
      361 cctcttattc agcgccacca gaaggcattc ttggtgctta cggtttcgaa tccatagctt
      421 gaaaaactct tcttgttgac ctcatataga tctggattga ggcgcaccat cactacctct
      481 agatttttct gaatgtaagt atcgacaaat tgggagatat tcttctgtct ggttttcatt
      541 ataaaatcgc tttcctgaaa aatcagtgat ggtattcccc agctccatag tgtggccata
      601 tggcaagcgg aactcatcgc gtctccttga atcgccatta tttcttctaa taacgcttga
      661 caagctcgat cataatcggc cgcggaaaaa taggatggat tggctttaac catataaagc
      721 tcggttccac gcacggcttc tttgaaagca aatacagttt cggcgggctt cttgccggct
      781 ggcacaatcg ataagacact gatccaggat gagtctgtgt taggtacata gtcgatcctg
      841 atattctcgg gttccgatcc cttaggcatg cttttgcgaa ctgccatctg aaatcggctt
      901 gtaggttcgt ttgcaagttg ctgcagcatc agcgctgcga aataggaagt agtgtccttt
      961 ggatccggac ccctgtatcg catctctatc ttcatgtgat ctgctgaaat gtcactgtca
     1021 ggaaaaacaa gaaatattgg cttcgtgacg cccggtctag gaaatggagc gagatttgct
     1081 ggccatggat tcttggcttt ttgtagcagg ccaaagcgtg cctcaatcaa ggacaaaacc
     1141 tgattggggt cgaaggcacc ggcaattgaa aggcaagcat tgttgggaac aagccaggaa
     1201 gaggcaatgt tctttagctg tgcagcatcc gctttttcca ggacattctc ggatcccaat
     1261 atgtcgattc tataaggcgc cttagcaaaa agcttcttat ttatggccat cgagcttaca
     1321 agatcattat cagaggtttc attctgaatg agattcaaga tagaccgccg agctaactct
     1381 attccgtctt tttgagcaaa gatgaattct cttctttcct gcgaaaagag atagagaaga
     1441 gtgtcaaatg cctggaccaa gttctctttt ttgaacgaaa taccgaattc aaatctatcg
     1501 atccccaatc cacctcttat atcctctgct tcaagcacat caatcgcggc tgcgggctcc
     1561 ggaataccag gattcgccgc gggaccatta aatagaacat actccaaaag cttgaataga
     1621 ccagcagttg aagctgtctg agaatttgct cctgcgcgat aggcaaggat tatggatata
     1681 tcttcttcat attcgatggg attgagacat actgtcattc catttgcgag tgtcttgatt
     1741 tgatattgtg cggaagcaga ggagcctttt gagagtatta aaaataggat cactatccat
     1801 agaaatctgc ggctatgttc cacctgctta tagtaaaaac agagcaactt gttgtcaatc
     1861 ttgaggcgta atttcgttac tatctatttc cgaatcgtct tctgtatggt attccccttg
     1921 agagaaatct ccaaaaagac gtttgcgaga atcttcgagc caatgagaaa cctcatcgcg
     1981 ggtaaaaaac atgtcctgct tttgaggatc gagaaatagt ttccatggct cgacctttag
     2041 tgcagatgaa atcttcaaga ttcctttact ggaaggaaaa ttcctgccgg tctctatatt
     2101 tgctataaaa ccaggagaga ggttagccag atgggcaagc tcttcctggg acatctgaag
     2161 aatagccctt ctcaagcgaa gattagaagc gaaacaatat cgcacgtaat ccatttaaca
     2221 tacactacct caatatgaga tatgtctccg caccgagagc atgcaaatca ccttctatga
     2281 cgaaggatcg tgccaataaa tgtaattcca tcctatatta ttctaagagc gattgaccgc
     2341 tccattggaa gtatttacaa ttaatgcatg ttcatcagag aaatatatat aaaaaggagg
     2401 agagcgttct tagacgctat ccgcgaccga ggaataacaa gtgggaaagt tctattgctc
     2461 gcaaatgaaa actgtccgcg caattatccc gaaaactact acccctttag acaggactct
     2521 tcctggttat acttgatcgg cctgaatatc cctagtctcg cttgcactct ggatattgag
     2581 agtggggaaa actgccttta tgctgatgag ccaggccttg aagagatcat ctggactgga
     2641 gtattacctt ctccatcaga gctggcctat agtgcaggca tagataaagt attgccaact
     2701 tcggttcttt ccaatactgt ggggcgagcg cttaagcaaa gagagcctgt ctttattctt
     2761 ccgccttatc gagatagaca aattgcaagg tttttggatt tatttgctgt ctctgtatcg
     2821 aacctagagg agcttatcta tcgccatatc gatccgcgca taattctggc tcttgtaagt
     2881 gtgcgtgaaa taaaggagcc tgaagaacta tccgaactcg agcgagcggt atctatcacc
     2941 aagatcatcc atgaagagct tatgcatgaa ctaaaggctg gatggacaga gaaagatgca
     3001 gctggttttg tgtcatctcg cgccagcgcg caaggctgtg aactcagctt tgccactatc
     3061 gcaacctgtc gcgcttcggt tctgcacaat tcccccaccg agcatgtaac cacagagcat
     3121 gatacatttc tgttagatgc tggtgtggag acgactttgg gctatgcggg ggatcttacg
     3181 acgacctttc ctgtaggacc gagctttagc catcaatcga gagaaatata tcaagtgcta
     3241 tatgagaccc tccagacagc cacaaatgcg cttgctcccg gggtgaggtt tatcgatgtg
     3301 cacaagattg cttgtctgac cataacaaaa ggcctcttat ctctaggcct tatgaagggg
     3361 gatccggtcg aggcagttga atctggtgct cacgcactat tctttcctca tggtttgggt
     3421 catcagatag gcttggatgt acatgacatg gaaggtctag gcgaagatta tgtaggatat
     3481 ggagatgaac taaagcgaag cgagcagttt ggattgagct cgctcaggct tgcaaagacc
     3541 cttaagccag ggattgtcca ctcggtcgag ccggggatat attttatacc gcagctcata
     3601 caaaaatggc gagctgagcg gatttgtgag agttttctaa actacgatat gatcgaaaaa
     3661 tggatgccga ttggtgggat gcgcgtcgag gaagattggt gcataaccga caaaggagcc
     3721 cggagattgg gtccggcatt cgacaggagc attgaagcta tacagaaagt cagggcaaat
     3781 agatgaagac agtattcgtc tccgcatttc tcgaggattc aattgttgta aagactatgc
     3841 tcgaatctgc tggcattcca gctgaagtac tctctgatca gatgctggat ataaacccct
     3901 tctattcgat agaaccaaaa ggagcaaagg taattgtccc ggatagttcg gaagaagatg
     3961 ccaaagcgat agtcgcagac tatagggaac acaaacagtc gaataagtaa ttctcaatca
     4021 ttttgcccat tattattagg aaatgtttta tgaaaagaaa gttgtttgtt ccgatcacga
     4081 ttacgctggc aagtttgctt ttgatcggac tattgatcta ttctattcag aggcatattg
     4141 caggtcggaa cttcgagtct gtcaatgatg cgctctcaag tatcattgag atacaagcgt
     4201 atgcattcta taacggttgt ctttcaaaca accagaccta taacttagtt atacaagggg
     4261 aatttgccga ggccaatgcc cttgtacaaa aagcaaccga ttcattttct ctcatagatc
     4321 tggcagaaat tgtggaaggc aaaccaccat cggatagttt tgatatcatt atggatggag
     4381 aactgttgcg catagagtgt ccaatccgca atgatgacaa gacagccatt gtcccaaata
     4441 tgattggcgt ggcgattatc caagctcaga gactattgaa tactgtctcg gctaaggatt
     4501 tcagaatcga tctagctcat ggccgcaaga ctacttatgg aattccaata tcggctaatt
     4561 ttaccgggaa tcgcttatgg gattccataa ttatagcgct attgacaggc tttgtagcga
     4621 ttttcatttt tcagcatttt tataggaatg caaacacttt tctggacact cgcggtctcg
     4681 aatctataat ctatctattt gagcagaccg agagaatctc agcgagccat tcaaggaatg
     4741 ttgccatgct tgctctcttt gttggtaaga atcttggctt tagaggcaaa agactcaagg
     4801 atctctatat tgccgcattg ctccatgata tcggcaaaat aggcatacca aacaacattc
     4861 ttactaaaaa cggcaagctc gatccatgcg agtttgtcca gatgagacag catcctgcca
     4921 tttccgcacg tattcttaga agcttcaagg aatttgagcg acttagccca attgtgctct
     4981 atcatcacga gagagaagat ggttcgggat atccggaagg tttgacaggc agggatattc
     5041 cattcgagtc taagatcatc gctgtagtcg atgtattcga agcacttata ggagagcgtc
     5101 cttatcgcga tcctatatgc ccgttaagcg ctttccagaa gatgagatcg atgccactcg
     5161 atcaaaaaat agtaagtctg cttatggacc actatggaga attggatggt tttaggccac
     5221 cgcgttgggc cctgtcttat agtccatgga tgctcacttc atgagaagtg cgaatatgag
     5281 cgctttgaag catgaatttt ggatccatcg gcttttctat atggatttct aagattacga
     5341 cgaatagact gatacatatt gcaagcatcg tagtatcgtt cggcattctt gaaactcgag
     5401 aattcatcac agcgaaacag ctccaacaaa acggaaaagc ggggatctct tatcagcgct
     5461 tctgtttttt gaacaggaag tattggtaat gcggctggca tcatgtgata tctgattagg
     5521 aatttgacat cttcttggat ttcttctgag aatcctagac gagaaagaaa tttctccgca
     5581 cgtttggctc ccagttcggc atgtctgtcg aagcgatggc ctttttggct tttagattct
     5641 gatttgccaa tatcatggag aagtagagca agcgagagag taatatcgcg ggtttttcga
     5701 tacgaaaatg tctgcatagt atggttccat ccattgccct cgggatggaa gtcttttgaa
     5761 tgatcgacat tgtcgagaag cgcgatttca ggccattccc gagcaataaa gccgctttgc
     5821 ttgagcacct cgaatcctga gactggatag ggcccagaga gaatgcctat tagaagatct
     5881 ctttgaaaaa gtacagatgg cgaaggcgga agagcgatgg atccaattgt tttcggaggt
     5941 aactcggata gggtagctag aagtgtgaaa tcgaacaatg atctttcaat ctcagaatat
     6001 tctgagctgg atggttggat atttctggct tttagagaat tgtatatatc gcctttatca
     6061 taataaacgc cagtagaagg gcttctttta aaattcatcc aagggctgcc cagattttct
     6121 tcgagcgcat cctcggtaca gataaaccgt acttcgtcgc atagagacgc atcggcgtaa
     6181 ggcagattag ggtattctag aacatcgaat acgcgggcta gctctaccag cgaacattgt
     6241 accgcaagga actttaatgg ccgaggtttc tcgtccaagt atgcatcaag cgcagtaatg
     6301 ccatattcga agaaaagcaa gtgtgcttct tcgagccgtg aataaatctc agtcattttc
     6361 cttgcatttt ccttgcatga caggaattgt cgaaatgagc ttcataaaga gctctgattt
     6421 aggatctgct cccaataaaa tcttgtgctc ttcattctct cccaagcgat atgtcaaagc
     6481 aaaaactgta aaaggagggc taaagagaaa gtcaaagaaa tgtcttttgg gcagttcaat
     6541 cgatgttatc aaggttaagg gcaagactat gtccgatgcc gtttccttag ggaccttagg
     6601 tgcggccatt ctaaagagcg attggaacca attctcgccc ggggtaggcc tgaagcggat
     6661 agacgaagcg gtcaatacga gaaggcccca ttcgccctgt gatttctttg agctaaaatg
     6721 ttgacccatc gtcttagaga taatcttgtc tccaatttcc tgttcgaaat tagcccagaa
     6781 ggcatcgaca tcggtttgca tcatgatttt ttcctttatc tgacacagtc atagtctact
     6841 ataaatacaa tttcatttca tctctatgca aacgactcat catgctatgg tttagtcatg
     6901 aaaacagctc attttgcgga cgatctatcg accggcccac atcctggcat tatcaatgag
     6961 aagatgctat ttttctatca tgaatagaat gggcggtatt ctggcaggtc taggttattc
     7021 cacaatattc ggcttttctt ttcttatgac aaaagaagcc ctagaagtgc tgaatccgat
     7081 ggaattgctc gctatgcgat ttatcattgc tgcgctgttg atgagcatcc ttgtcattgc
     7141 agggataata caggtggatt ttaaagggaa acccatcggg cttctggcgc tgatgtgctt
     7201 attccagcct attgcctact tcacctttga gacctatgga cttgctaatg cggcgacgaa
     7261 tgttgcaggc ataattcttg gagcattgcc tgcaggtgtg gcaataatgg aagctttgct
     7321 gttgaaagag cgtctcagcg ctcttcaatc agctggtctt tcgatgtcga tagttggggt
     7381 tattcttgtg gcggtatttg gacaaggtca caataccgag acaaagccta tcgggattat
     7441 attcttgacc ggatctatgc ttagcgcagt cttcttcaat attgccagta agaaagcggc
     7501 agaaagcttt tcgccggtag aacgaacctt tgcgatgatg tggtccgcag ccatttgttt
     7561 ttttatacct gcgctctttg agaatatcaa gggaaagggc aatataagcc atttgtttgg
     7621 tgaatggaat agaatagcgg gagtctgggg cggaatactt tatctaggca tactctcctc
     7681 tgtgggagca tttttcctca taaattattc tctctctcaa ctcaaagcct cacaatctgc
     7741 ggtgttcacc aaccttgtaa cggtcataac ggtgctggct ggagtcttga tccgaaaaga
     7801 acaatttagt tggagccaag gattgagtgt aattctgata atattgggca tagcagtcgt
     7861 gaactcgtcc ttcaaagaga aagcgaaatc ctaaggcgca atttagctga ggaggcacaa
     7921 tgcaagatgc tgcgttctat gacattcatt gtcatcttct taatctatct catccagcct
     7981 ttgtatcgat tatcgaatca atgcgccata ggcctagaga ggttatttat tcgcagattg
     8041 catcattcga ctatctcgct tcttccctgc tcaaaagggg cggtgagaat ttaaggaacc
     8101 ttctggctgt aatggataat gattgcgctg acatactctt tttgctagag gatgatctct
     8161 ctggccaatt caaggagcca aagaagacac caggctttag gcaagcaccg cctttgcgca
     8221 atgggaagct ttatatagca gatcagcagt tttcttcttt cgtgttaact cctcttatca
     8281 tggattttct cgccccttca tccttttctc cggaaaccta ctataatcgt ccaccacaaa
     8341 aatggatcga agcccagatc atagatgtat tatttgcgat ttgcacatac cgggagaaac
     8401 gaccgtcggg ctttctgaaa atctatccct ttctagggat aaatacggga cattatacgg
     8461 cagaggagct ttctgctttt ctcgaacagt ggttgggcaa ttatacaaag agcgaaaatg
     8521 atatcgaaag gcgattcctt tcttattctt tcgatgcgat tgctgatttt cagagttata
     8581 gtattttttc cggaataaaa ctgtatcccc ctatgggctt tgatccatgg ccagaggatc
     8641 cggcggagag ggaaaaagtc gaattgctat atagctttgc cgaaaccaaa ggagttccta
     8701 ttacaactca ctgcgatgat cagggttatc gcatcatttc cttggaggaa tcattgaaat
     8761 ttactgctcc atccagatat agacccgtcc tgcaaaaata tccgaggcta attttgaatt
     8821 ttgctcatat gggcaagcgc catattcgaa ccatacgtgg caacgaacag gcagattggc
     8881 gcaaggatat cttcgactta attatcgaat acccaaatgt atattcggat ttttcattta
     8941 cggccggaga accttcattc tatgaggagc tggcttctac gttaaatcac ctaaaagagc
     9001 ctgagcagga aaaaataata cagcgaattc tatttggttc agatttcatg gtgaatcttt
     9061 tcaaggttcg ttcctataga gattatctgg agtatttttc tgagagtgtt ctaagcgtag
     9121 agaaaaagcg ccttttctgt tcggacaacc cgcgaagatt tttgttcggc gaccaaaaag
     9181 cataatagat cagcgaccaa taagcataat aggnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnntttttgt tcggcgacca aaaagcataa tagatcagcg accaataagc
     9361 ataataggca atttcaagcc taatcaagga gcatctttct gcagaattct tcttataatc
     9421 caggaggcca tcatatggcc tgctgtttgg ggaacaaaag gcatcgaacc ttgaatgatt
     9481 cttttggttt cttgaacgat aggttttgta ggaggctcgt cagaccatac tacaggaaaa
     9541 tcgagaggaa tctccatttt gccaagtcgc aggcggactg cgcgggcaag agcgcagcct
     9601 ttcacctctt gcattttgcc aagcttgatc gactcgggcc ttattcggcc tgcggtcccc
     9661 atgctagata tgaaagctga gcgctcgcgg tataaagagg cgataagaat taccttaggc
     9721 atgatagaat ctatgcagtc tgcgtgaaaa tcgcactcgc gagcaattat ttgtgaagca
     9781 gattcagctg agaaaaatga tcttacactc atgatttgga ttgcagggtt tatttctttg
     9841 gctgcctggc tgaatgcctc tgttttagct aatcctacat atctattata gccaaaggcg
     9901 agcctattga gattcgagta ttctacaatg tcataatcca caacatggag atatccgact
     9961 ccagcgcgaa caagatccaa tgcgcaagca gctccgacac cgccaagtcc gtagactgca
    10021 actttggaat ccgcaattct ggcaagtcca tcatcaccag ctattatttt tgtcctatcg
    10081 agaaatgatc cttcgtgttt tttgaaagga ctggtttctt caaagggttc tgtcatgtat
    10141 ttcgctccgt tcttcatata ttctatactg ctacgaacct tgtttataag gattgagaat
    10201 ggcattagga agccttctca tgcaattttt atacacaagg ttcgtatcag cagtgaagat
    10261 atgcggccga gaaatttaca gagagctcat gtctcaattc ctcagagctc atattgagaa
    10321 cggaggacat cttttcgaca acattggtaa tgtgttctag gcttgtatat tcggctccat
    10381 gaggcggttg ccagggtgca tctgtctcgg aaagaattcg tgaagtaggc aggcccacca
    10441 aagactcgat cgcgtgcctc gaatctctca ataatggtgt gccaaaagaa aaataagcgt
    10501 ttattccttt tttcaaaatc atctgagctt cttcgagtct ccctggccag caatggaaga
    10561 tgatcgaagg caatcgccta agctcgcgtc cgtaagccat tatgacatca gtcgcttttc
    10621 ttatatgaat tacgagcgga agttgggtat aagccgacag atgaacttga aaaagaaaag
    10681 cctcgagctg caagtgcatg gcttcggagc ttcttgaccg caatggtcca tcaccgaaga
    10741 aatcgaagcc agcctctcct atgaaatcta cttttctttg ctcacataac atgctgagaa
    10801 aatctgcgtg ccgcatgtct ggttcttgag gatgaattcc aaatccggca atagtagaag
    10861 gcaagttggc tcgcaaggcc tcgctttgca gaaattcttc agggttatga gatacaaccg
    10921 cgcctcgcca atcaaatggc agagctctta gagcaaagtc aggttcatat tgctcgaggc
    10981 ctaccaagtg taaatgcgca tcgcagtaca tgacaatatt gtgcaactat aaagcatgct
    11041 ggtcaacgtt tgtatcatct tctaatagac cgagttcaat aacgaacaga tcgaaatggg
    11101 taaattcccg agaccgtaaa atcgtagctg ccaagtcaag cttactattc ttttgcgcta
    11161 tgtcgtttga gcttctttgt agtagtcatc tcgagcgggg ctttgagaat atgaatccgg
    11221 gtgatcttct cataagagcg cagcctttgg tttacttgat ttacgatgtc atgaatccgc
    11281 gtttcgacct gctcttggga aaggttattc tgcgaaaccc agtcttggtt gggataaata
    11341 aggctttcga tttcctcagc cactgccttc ccttcaggta tatagcctcg aacaaggatc
    11401 tggtctattt catcaaagag ctggaactcg ttttcgattt cttcaggata gacattcttg
    11461 ccaccttcgg tgacgatgac attctttatt ctgcccgcaa ggtatagata gttctcatcg
    11521 tctaattttc cgacatcgcc agttcgcagc caaccgtcag gagaaaggac ttccgctgtc
    11581 tcctctggca ttttgtaata cccctgcatt accattggac cgcggaccac gatctcgccg
    11641 ataccatcct cgttgggatt atcgattatc atttctactt ctggaatcac cttcccaact
    11701 gacgtctctt tataatgctc taccggattg agagttagga ttggcgaggt ctctgtaaga
    11761 ccatagccct ggacaaagtc tatgccgagc tggttgtact gcctgaatac ctttggatct
    11821 agtgggccgc ctcccgaaat acagatgcga atcgaagata ggcttgcttt ttcgaggatt
    11881 gaaccaaaga aagctcttcc aggatttaca ccgaaagtct ttttgataaa gcccgaaatt
    11941 gccatcaaag tcctaattaa gccataggca agtgcacctt tttccttgac tcccttcaga
    12001 atacctgtta ggagcttatt gaagagcatt ggcactccga ggaacatggt gatcttggct
    12061 tctttgagat ccgagagaat ttgtttgaca accatgcgct taccaaagac agtctccgct
    12121 ccgaccgaga aagactcgat aaagactgcc agcatcgtgt acgaatggtg gattggtaaa
    12181 agtgcgtaga atatatcttg ttcggagata ttcaggtttg attgagccaa gaaacagtca
    12241 gagacaaggt tctcgtggct aagcatgact cccttgggat cgcctgttgt gcctgaggtg
    12301 aaaagtatgg ctgcggtgct ttgtaccgga acttgaggag gggtaaaaac gaaacccgcc
    12361 ggtggctcca aatcatagat aaagctttcc acacctctgg caaggccaaa acaggggatt
    12421 cctgcaaaat ctttcttgat atgctcgtat ttctcttcgt cgacgaaggc tgccgttaca
    12481 tcgccagcat gcatcagtgt tattatatct tgtgtcgaca gctgatagtc tatgggaaca
    12541 acgactgcac ctgtgtagag aactgcgata taagctatgc cccattctgg agagttcttg
    12601 cctgtcaccg ctatatgact acctggtcct aggccaatcg aagtgagata cgaggcaacc
    12661 ctcaggataa tctcacgagt ttcgcgataa ttgaggctta ctctgtctgg gctatatatc
    12721 gtaaagcatg ctcgatcggc aaagcgatcc gtagtgatgt cgaatagttg tggaatagta
    12781 ggccatttgc tagtaaaaat acgacctcga tatgcatcta ggaatttcca aggttctgtt
    12841 tctctcatag cagacattat gatacagaaa tgcggcgttg aaaagaggtt gaaacatgat
    12901 tttgccccct tccaagtggg ctcagttttg cggtattctt ttgtgcactt tcaatcattc
    12961 gatcatattg atggaatgag tgcaatgcgg aggagagcat gaaatcagtc gagcttgcaa
    13021 aagcttacga ccccaaatca ttcgaagagc gcatctacga aatgtggaag cgcgaaggcc
    13081 tttttaagcc aaagatcgac aagaacaaaa gaccctttac cattgtaatt cctcctccca
    13141 atgtaacagg ggctctccat ctggggcatg cactagataa ctgtctccaa gatatccagg
    13201 ttagatatcg tcgcatgaaa ggaattccga cactatggct gcctggtaca gaccatgcgg
    13261 gtatagctac tcagaatgtg gtggaacgca agcttcgtaa agaaggacga gaccgtgagt
    13321 ctatggggcg cgaagctttc atcgaagaga catggaaagt ggcgagagaa catcgagcct
    13381 ttatcgataa tcagcttgcc agaattggat ctagtcttga ttggagcagg gaaagattca
    13441 ctctggatga aggattatcg aaagctgtgc gcgaagtctt tgtagcgctc tacgaacgaa
    13501 atctcatata tcgcggcgaa tacctggtga actggtgccc atcctgtggc accgctcttt
    13561 cggatgacga ggtggagcat gaggatgaac caggttctct atggtacatc tggtacgagc
    13621 tcgtcgatgg gccttgtcgg gaatgtccga gtggcaggat cgagatcgca acatctcgtc
    13681 cagagacgct gcttggcgat gccgctgtag cagcccatcc cgatgataat cgctatcgtg
    13741 ctataatcgg caagaaagtt catttgcctc ttacagatcg tataatcccc ataatcgagg
    13801 acagctatgt cgatccagaa ttcggcacag gtcttgtcaa aataacacca gctcatgatc
    13861 cgaacgattt cctcgttggg cagcgccatg gactcgagaa tatcaatatt ctcaatccag
    13921 atgcgacgct atctgatgct gtgcccgaga aatatcgcgg tttaagtgta cttgacgccc
    13981 gcaaagcagt gcttgctgat ctaaaagcac aaggccttct aaaaaatgaa gaaaaaatcg
    14041 tgcatgcgat cggtcattgc tacagatgcc atagcgccat cgaaccttat ctctcgaagc
    14101 agtggtttgt tagaatgcaa ccccttgctg ataaggctct aaaggcttgg caggatggag
    14161 atgttgtctt cttcccgaaa aagtgggaac acacctatcg ccattggctg gaaaatatca
    14221 gggattggtg tatctcacgc cagctttggt ggggacaccg cattccggtt ttctattgca
    14281 tagattgcgg tgaaatgatg gtttgccgtg aagacccctc tgtctgcata cactgtggct
    14341 ccagcaacat atatcaagat gaagatgtcc tcgatacatg gttttcaagt tggctttggc
    14401 ctttcagtac acttggctgg cctgaggata cggaagacct gagatatttt tatcctacaa
    14461 gcgctttggt gacaggatat gatatcattt ttttctgggt agccaggatg atcatggccg
    14521 gtatggagtt tacaggtcag agtcccttca aggaagtcta tattcatggc ctaattcgcg
    14581 ataaacaagg ccgaaagatg tccaagagtc tgggaaacgg catagatccg ctcgatatag
    14641 tggaagaata tggcgcagat gcactgaaat tcaccctggc ctataattgc gccgctggcc
    14701 aagatattct cctcgataaa gaaagtttca aattaggcag taaattcgct aataaggttt
    14761 ggaatgcctc gcgctatatt ctgatgaatc tcgagaaccg tgcttttctt gaggatgatg
    14821 ccctcactta tagcgacata gattggtgga ttttgcataa gctaaacgaa gccgcgaaag
    14881 cagccattca ggctttcgaa tcctggcgtt tcaatgacgc tgctcaagca gtctattcct
    14941 atttttggaa cgacttctgc gattggtaca tcgaagcatc aaaattatcg actaaatcgg
    15001 gaagcgaatc agaaaaagat cgctctacta caatgctcct tagagtactg gaagagtcac
    15061 tcaggctttt gcatcccttt cttccgtttg taaccgaaga aatctatggc atgctaccaa
    15121 atgcaaaggg acggctgatt atagcatcct atcccgtgtg gacaaaggat cgcgaatcgc
    15181 ctgaaatcga atcacaattt aaagcacttc aagagattgt tactttggtg cgtacactta
    15241 gaagtgagtt tcagattgcg cccgagattt caattcctct atctcttgag tttgataatc
    15301 atttttcgca tggtcaattc atacagagtc atattgagct cataagccta ttggcaggtg
    15361 cttcttcggt atcgataagc aaggccacag aaaaggggag tgtttctttg gtaggtaatg
    15421 gctttactgt ccatgtgggg gtgggaggtc ttctcgatat cgcaagactg attaatcgcc
    15481 tttctaagga ggcggaaaga gagagagcat acatcgaaaa gctcagagca aaacttggca
    15541 atcggacatt ccattcctct gctccctcag atatcatcga aaaagaaaag gaaaagctca
    15601 ggctatctat ggcaaaggct gcaaagctcg agcagtatat cgaggagctc tcatgagttc
    15661 agaagagggt atccctcaag tagacattcc catcataggg cggaattcaa cacttcgtgc
    15721 cgagcgcctg ataatgaata agacagcgat gctgcgccta aagcgtatgc atcttgcgcc
    15781 atatattcag ctagcgacat ccttaatcgg aaagcccagg agaagcggag gcaacatgtt
    15841 caggcatcaa ctggacacga tggccatcct aatcgattat ggatatatcg attctgtgct
    15901 tctaaaggct tctttaatcc atgatctact cgaagacgca ccggaaacga atcctgattc
    15961 cattctttcg attgatgatg agtccataga tgtatataaa ttggtcctcg aggttacaag
    16021 acggccagtc gaaaacaagg ccgaattctt aattcgaatt cgagattatg gaacaccgca
    16081 cgcaagagtg ctgaagagcg ctgatcgcat ttccaacatg atcagtcttg gttatgtgac
    16141 agacgatgta ttcgttaggc gatataccga tgagacagaa caatatatct atcctattgc
    16201 cgaacgggca gatgagcgaa tgctgatgga attgagagag ctcgtcgcta ctcgtcgaga
    16261 gtatttgcgg aacagaattg agatataata aagaaggcgc cgatgagggc agaaggaaag
    16321 taacatgaaa aagaagtgga caagcctaat gcttgctaaa gtaatcgatc atacgcttct
    16381 caaggctacc gcaaccgaag atcagataaa agcgctctgt gtagaagcaa aggagtttgg
    16441 attcaaagca gtctgtgtca atccttactg ggttccgctt tgtgtcagag agcttgcaga
    16501 ttccgatgtt ctcgttgcga cagtcgttgg ttttccgctt ggcgctaacg ccagtctgat
    16561 aaaagccgaa gaagcaaggt tggccgtagc tcaaggagcc gacgagatcg acatggtcat
    16621 caatattggc aaggctaaag ccggagactg ggctgcggtt agggatgata tagccggagt
    16681 ggtacaggca tctagaccag ccattgtaaa ggctataata gagacctgtt acctaaccca
    16741 ggaggaaaaa atcgctgcat gcgaagcagc catttccgca ggagcaactt ttgtcaaaac
    16801 cagcaccggt tttgggaccg gtggtgctac cgtagaagat gtcagcctta tgaagagaac
    16861 cataggcgaa gtcgctcacg taaaagcatc aggcggaatc aagacttgcg aggatgttgt
    16921 atcgatgcta gaggctgggg cctcgcgcat aggagcctcc gcgggagttg caataatgag
    16981 ggagtctgaa gctttagata ataaatcgcc agaacagctg tcataacgca aagattgtta
    17041 ttgacaatct gctgcctcgg ctttaccatt tatttgattt tatgcggtag attgatagag
    17101 aatctacgca tttccagcat cgaacctata atgaaggagg atgaagcatg aaaaaagcgc
    17161 ttttggtgct tgtcgtaacc gctttgatcg cggtgcccgc atttgcacaa ttcaaagtgg
    17221 gcctcgttac cgacgttggt ggtatagacg acaggtcctt taaccagggt acatgggaag
    17281 gtattgttcg ttttgctcaa gattacaaac ttccaaagag caactacaag tatctgcagt
    17341 caagcgcgga agcggattat gttcccaacc tatccacttt tgccgatgaa ggtctcaatc
    17401 tcatcatagc ccctggattc ctgttcgagc aagcgatgac ccaggtcgca aaaaattacc
    17461 ctacccgcaa gttcttaata atcgatacgg tcgtatctct gccaaacgta gtatccgcta
    17521 cctttgcgga gcatgaagga tctttcctgg taggtgtggc ggcagcccta aaggctaagg
    17581 ccgatggtaa gaacatcctc ggattcctgg gtggcatgca gttcccgctt atcgaaaagt
    17641 tccaggcagg attcgagcag ggtgtcaagg cagtctatcc ggactgcaag atcttagtag
    17701 actatgcagg ggattttgct gcccccgata aaggtcaagc tttagctcaa aagcagtata
    17761 acgccggagc ctacataatc tatcatgcag caggcggtac cggtaacgga ctcatcaagg
    17821 aagcaaagga acgccagcag aaaggcgatc tccgctgggc aatcggcgtg gacaaggatc
    17881 aatatgccga tggcatctat agtggcaaca aatccgcagt ccttacatcg atgatgaagc
    17941 gtgtcgatgt ggcagcctat gaagtgagca agatgaccat ggaagggaaa ttcccgggcg
    18001 ggcaagtcct tgttttctca ttgaagaata agggtgttgg cattcctgat aataacccaa
    18061 acctctccgc tgacatcgta gcaaaagtca aggaatacga agcacttata gcatcaggca
    18121 agcttgtcgt ctcggagatt cctgcaaaat aagcagtcta agcaaaaagg gggaagcgcc
    18181 cgaatcggcg atctgctaaa gctcaaaatt gccgaaaaag cagctctgtg tggacggctt
    18241 cctctatagt tgtttcaaaa gcccccaaaa gagggggctt ttttgatgcg tttcatcttg
    18301 aattggcgat tccataaaga aatcatttca aaatcggtct ttgaccgatg tataatgtgt
    18361 gctggttata tagcgagaat agtcgcgctg tgatataatt ttgcttcatc ctaatccagg
    18421 ggggttatag gtgcctaaca tcgtagagat gcttcatatc accaaggatt ttcccggaat
    18481 tagagcaaat gacgacatca cacttcaggt cgaagagcgc tcgattcatg cacttctggg
    18541 agagaatggt gctgggaaat ctacgctgat gagcatactt tttgggctat atcagcctga
    18601 tgcaggaaca attcgcattc gcggcaaaga ggtaaaggta agtgacccca atgtggcaac
    18661 cgctctcggt attggtatgg ttcaccagca tttcaagctg gttcataact ttacagttac
    18721 cgaaaacata atactcggca tggagcctcg aaagggtatt cttgtcgata tgcataaggc
    18781 agaacaaaaa gtagaagaaa tctccaagct ctatgggctt gcggtcgatc ctaaggcgaa
    18841 gatcgatgag atttcggtga gcatgcagca gcgagtcgaa atactcaaaa tgctctatcg
    18901 agatgccgag attctcatct tcgatgaacc tacagctgtt cttacaccac aggaaattcg
    18961 cgaactcatg cagataatga ggcgactggt cgaagaaggc aagaccattc tcctcatcac
    19021 tcacaagcta agagaaataa aagaagtcgc cgatgtatgc tctattctga gaagaggtaa
    19081 gctcgtcggc accgttcctg tcgcagatac ggatgaagag agaatggccg aaatgatggt
    19141 cggccgccag gtcagttttc atgtccaaaa aactcgagca aagcctggcg atgtacttct
    19201 gcgcgtcgag gggctcaatg tgaagaatca aaaaggagta cttggcgtaa gaaatctatc
    19261 actctcggtt cgcgctggcg aaatcctcgg gctctgtggt atagatggca acggccagac
    19321 agagctcgta catgcaataa ccggattgac taaggttgaa tctggccata tcttccttcg
    19381 agatatcgat ataactcacc tttctataaa tgaaaggtct gatcttggtc ttggacatat
    19441 accagaagat cgccagaagc atgggctcat cctcgacttc acattgggcg agaatttcgt
    19501 aattcacaac tattataaag aacctttcgc gcattggggg atactgaaca agaaggcaat
    19561 tctggataac gctcagaggc ttatccggga attcgatgtt cgttctgggc agggacccct
    19621 tacaccggct cgcgcgatgt ctggaggaaa ccagcagaag gccattgtgg ctcgcgaaat
    19681 cgatcgttcg cccgatgtct ttattgtggt tcagccgacg agaggtctcg atgtgggagc
    19741 gatcgaatat atacacaaac gtatcattgg agaaagggat aaaaacaagg ctattttgtt
    19801 agtctccctt gagctcgatg agatcatgga tctctctgat cgcattgctg tcatctacaa
    19861 cggagagatc gtcggcatcg tcgatgcagc aacgaccaat gaaaatgaac tcggtcttat
    19921 gatgtcagga gctcttcata aagcacatga agcgactcag actggccgag aaccagcgca
    19981 tgtctgaggg aagggaggaa ggcaaatgcc tgctcgaaaa gaaaagatag tgcttccact
    20041 tattgctgtg cttcttggat tcttgctggg atcattaatc gtcatactaa ccggtcgttc
    20101 tcctgtctct atgtatgccg ctataataaa aggattttca ggcatagata tcataaatgg
    20161 acagccgatc aacacgcgtt atataggtga attcataatc caggcaatgc cgataattct
    20221 aacgggtcta tcttttgcct ttgcgtcgcg caccggcctt ttcagcattg gtgccgaagg
    20281 tcagttgatg atgggttcca tctcggctac ggcagttgcg attcttgtgc aagcacccaa
    20341 ggtgattcac ctgccgatgg tgttgcttgc ggctattctg ggaggagcgc tttggggtgc
    20401 cattcccggc tttttgaaag cgcgatacaa cgtacacgaa gtagtagtta ccataatgat
    20461 gaattacatt gctctgcatt tcaataatta tatgattctg aacatttttg ggagcgtgga
    20521 taaggtaaag acagtgcctt tccccgattc cgctctgctc aagtcgccat ttctggagag
    20581 cataactcgg aactcacgcc tcaattgggg attcattcca gttctattag cggttctgat
    20641 attctccttt atcataaata agactacctt tggcttctct ttgcgcgccg taggtcacaa
    20701 caaggaggcc gctcgctatg ctggcatgaa ggtgaaccga aacatcatcg catctatggc
    20761 aatagctggt gcttttgctg gtcttgcggg tggaataatc actgttggca ctttcaattt
    20821 tggtcgtgtg attcaagcag cagaaggcta tggtttcgat ggaattgccg tggctctagt
    20881 gggaggaaat gaggcattgg ggatcttgct tgctggtctt ctgtttggag ggttgaaggc
    20941 cgcacagcct ctcatgcagt ctcagggcat acccagagag atagcaggga tcattcaggc
    21001 ctcgattgtg ttattcgttg caatgaaatt tggcataaaa atggtgctcg ataaattcga
    21061 gcgaaaacag gtgcccacaa cggaagggga acgcgtatga ccttcgaggt attccttcag
    21121 atgtttccta ttgcgctcat gttttcagcg cccattatat tgactgccct agggggtctc
    21181 ttcagcgaaa gatccggcat cgtcaatatt gctctggaag gaataatgat ggtaggtgcc
    21241 tttactggcg ctaccataac cgtccttttg gagaattcga ctcccgcggc ggcgtggatt
    21301 ggattgctcg gaggccttct atcaggggtg gccttcagct tgctacacgc cgttgcgagc
    21361 atcaatctca aggccgatca agtcatttca ggtacagccc tgaatattct ggccggaggg
    21421 cttacggtct acctttgcca gatcattttt catcaacaga ggactcgtgc attttcttat
    21481 ggcataaaga agttgagtgt ccccggattg agcaagattc ctgtgcttgg aaggatgttt
    21541 ttcgaccagg tgtaccctac gttctacatt gccatcgcca ttgtactttt gacatggttt
    21601 gtggtatata agacgccgct tggtcttcgt atgagggctt gtggtgaaca tcctcaggca
    21661 gccgcgagca tgggtattaa cgttgcaaaa atgcgctatt ttggcgttct gatttcgggt
    21721 gctttgggcg gacttggcgg agccgtaatg gtactgacgg cggatatcca atataccttg
    21781 gtgtctattc atggcacagg ttttatttct ctcgcttcgc tggtcttcgg tagatggaat
    21841 cccttcggag tgctcggagc tggattcttt tttgggtttt ctactgccct ctcattctat
    21901 gcgaaagata ttccgctgct ctctgggctt cctggagaat ttttctatat tctgccttat
    21961 ctgcttacca tcatcgctct tttattcttt gcaggcaaat cggtgggacc caaggctgct
    22021 ggacagatct acgaatcagg ggaacgttga aacaagcata atattctgaa ggaagcaaga
    22081 aatgatcgag gtcgaccagc ttcaaaaata ctcgctcttc ggaggcatca gtccggaaca
    22141 gatagaaagg atcaagcctt tgcttggtac agagcatttc gaagccggag aatgtccaca
    22201 ggtcgaaggg cagcccaacg ataagattca ttttattcta agcggggaag ttgaaataat
    22261 aaaaaagggg ctagtcatag cccgcctaag ggaagggcaa accttcggcg agatggagct
    22321 tctagatatc atgccgagta tcgcaacagt tcgcgcggcc actgctcttg aaaccgttac
    22381 aatctctaat cgtgccctct acgatatctt caaactcgat ctgagagctt tctcgatgat
    22441 gataatgaat ctcgcgcgcg atctttcgcg gcgcttgcga cggatggatg atattgcatc
    22501 cagccaagga aaatgttgag attcgatgca aagaatccca aagccaataa ttcttttctt
    22561 aaccgacaaa tgagcacctt cttaaaatag ggaagaaggg agtatcttgt aggcaataaa
    22621 tagtacaatt ttataaatta ttataaaata attaaataat atcttttgcc ggagacgggc
    22681 atcgaacccg cacgccgctt gcgcagcaag ggattttaag tccctggtgt ctaccaattc
    22741 caccaccccg gcaattcgaa tctagatttt agccagcaca taaaaagcag tcaagaagac
    22801 aatcaaagta tcgaagtcac tcaaggccat gttgtggata atgccttctt tatggcttgt
    22861 atagccgatc ttgtataaca gatcaaatca tattccgaaa agctcttcca gaaaacgcgc
    22921 cactccatca tcatccacag aggcggttac ataaggcact gctgtttgaa tatagctatg
    22981 ggcatttccc atagcaacac caaccttaac ggatcttagc atttccagat cattttcagc
    23041 atcgccaaat gcgattgtct cttcaaggct accgcccaaa taacgtagac attcctcaag
    23101 accttgccgt tttgaaacgc cgcttgcaac gatttccagc attgtctcat gagaaaaaaa
    23161 cgcatcaaga aggtcgggtg ctgagtttaa tatcgtttcc cgcatctcga gaagtttctc
    23221 atttgggcca actgcaagaa atttcaaaat atcttcgcca acgagagagt cgaaattctt
    23281 ccgaacacct ttcatgccag ttcgcttttc gtaaaaatcc acacctggcg aatagcgatc
    23341 gtagatccat cgttctccaa catagcaaca gaaaagcaca tcatggtctc tgcttacttt
    23401 tgtgagtatc ggcagagcct cggtcggaat atgatgtgaa gccaagatcg taccgtcgcg
    23461 gttatagata tcggctccat tcgtgcatac gaaggcatct ggttcaaagg aatccgcaag
    23521 gacaatcttt gcggccttat agatgcgccc ggttgcaatg atcaattttt tcccctggtt
    23581 ttggagcttt ttgagtgtat cagccacatg gggagaaagc tgatatccct catcgagaat
    23641 ggttccatcc atatccattg caatgaatct gagtttagat gggtctaggt ttttcgaggc
    23701 tgtcatgtgg tgataatata tgaaagttgg taattggtca ttactgctac gaaccttgtg
    23761 tataagaagt tgaagtaaga tgtaagaaat atcccgaata agggaagagc aaggggctga
    23821 ctatgcagac tttctctttc tgccataatc ccaaatgtct ccatatacac aaggttcgta
    23881 gcagtattat ttacatgccg acatagaagc ggcacaatgt tctttcatga catacaacca
    23941 ttatgatgac acctatattc tcgaaggcgc agaccttgta cttccggatt cgatattgcc
    24001 aaaggcgacg ctggcagtac gaggaggaaa aatagctgca ataatccctt ctgggcaatt
    24061 ccagcggccg gaaaagctaa atcgatcttc tcaatctcag attgacccag catttgaaaa
    24121 tgctcccgta atcaaggccg aagatgcttt tgtgcttcct tccctagcgg agatgcacat
    24181 ccatggctgt gcatcttggg ggttcgagag aatttcagag ccaggggatc tttttcaaat
    24241 agcagctctt cttgaggcaa aaggagtagg atgctatgta ccgacgattc tctgggagga
    24301 aagaaccctc attgagctta cagagacaat cttggcgagc gggatttctg cgcctctgct
    24361 tcctgggata tatctcgaag gtccatttgt aaatcattcg aagcgcggtg gtattatgcc
    24421 agataatatt cgagcacctg acatcgcgct cgcagagcat attctcgaca tatccaaagg
    24481 attgctgaag atatgtacaa tggctcccga gctagacggg atcgaccaga tatacccagt
    24541 tttccagaat aaagccgttc ttgtttcact tggtcattca gatgcgaaat tatcttcgat
    24601 ccatcctccg tctcgacctt tttccatcac tcatctattt aatgcaatga caggaattga
    24661 tcacagagaa gggggactcg ctaatttcgc tttttcacaa tctccagact atattgaact
    24721 aaatggcgat ggaatccatg tgaacgagag ttgtctaaag ctctgctctc gcttgattcc
    24781 tgagaattcg ctaatactga tttcggatgc cgtcatcgga gctgggcttc cgtatgggga
    24841 attcagctat tatgggagga gagttgtctc ttctgagcga ggtgtgaggt atgcagattc
    24901 gggtgtcctc atgggctcca atatgctggg aattgatatc gtaaagaact attatttaaa
    24961 ggcagaagtt ccactctggc gcgcagtccg agcgatgtcg ctaaccccgc gcaaggcgct
    25021 ggggatggat ggagaatatg gttcgataga agttgggaaa atagccgata tttttatctg
    25081 ggacaagggt ttggagactg ccgtaagacc caaagcgctt ctggcttcag aaaatgacta
    25141 taagccaaac gatttgctca gtagtaaatt taaggtacat agggtacaaa agaaggattg
    25201 agtcatggat catcgtgcaa cgacggcatg gatcgatctt gccgccatcc gagcaaactt
    25261 tcgccacctt agagatatag ctcagggtag cattgtctgc cccgtcatca aagccgatgc
    25321 ttatggacac ggggctctgg caatagcgca tgagctggaa cgcgaaggag cacagttttg
    25381 ttctgttgcc ctggcaaaag aagctctcga tctgagagag tctggcattc aaatgcctct
    25441 ccttatcttt ggtgcgccag agcattcctc atcctccctt gcaatacgcc ttgaatgtgc
    25501 tcagacaatc tataccgaag agcacgtgcg tatactggct tccgccgcta aaagcgtggg
    25561 gaaaaaagca aaagcgcata tcaagataga tactggcatg aatcgtcaag gcctcccatg
    25621 gacaaaagcc agatcatttg cggggctgct tgctgactat ccggaaatct tcgtggaagg
    25681 catatactcg cacttcgcag atgcggacag tcccgatcct tcgttcaccg atcttcagat
    25741 gaagcgtttt tccatagcgc tcgaaagttt tgaacgggtt ggaattaccc cgaagtatcg
    25801 ccatatagcg aacagtgctg gaatggttct gtatccaaaa acaagactcg atatggtgcg
    25861 gcctggtatt ctgctctatg ggctaagacc tgcgccgaat atagagcttc cagaaggttt
    25921 cgagcctgcc ctggctctta cgacaggcat cgtacatatt agagaagtac ccgcagaaga
    25981 atcgattagt tacggtcgaa ctttcactac aaagcgcttc agcagaattg gcctattgcc
    26041 aatcggttat gcagatggtt atccgagggc actttcgaat cgtgcctcgg tattgattcg
    26101 gggagttcgc gcgcctgtgg ttggtcgtgt atgtatggac atgacaatga tagatctgac
    26161 tggcatagcg ggagcgcgag ttggcgatga ggttttgctt tttgggggac ggactcttcc
    26221 agtttcggag atttcgaatc tgctttccac gatcgattac gaggtagtgt gtatgatctc
    26281 gaagcgagtg ccccgcgtac acatagattc aaaaccgcaa gaggaataat tatgaaaatc
    26341 ttacaacagg gttttcatgc ccataaatat caggccttgg gcaacgatta tcttgtcatc
    26401 gatccagcgg aatttcagat gtcgttttca gcttctgcaa tctctgccct atgcgataga
    26461 aaccgcggag tgggctctga cggaattcta tatgggccta ttctgtcgca aacttccttg
    26521 gtgatctgta gtccattcgg gttgcgtata ttcaatccag atggctcgga ggcagaaaaa
    26581 tcgggcaatg gcctaaggat cttttcctta tatctctatg agcgcggatt tgtaggaaaa
    26641 gaaccttttg aagtgcagac caagggtggc atggttgagt gccgaattct agctacaaat
    26701 agcccatgtt ctggagaaga tggctatagg aaagatgacc atagagaaga aggctatgaa
    26761 gaagagcatt atgtcgaagt atcgatggga gaagctagat tcttaccggg tagttccacg
    26821 cttgctatcg atggcattga gtttcgcacg ataagggtat cgatgggtaa tccacattgt
    26881 gtgcttttag gccagtatcc cacagaagag cttgcgcgcc ggatcgggcc tttagttgaa
    26941 aatcatcctg attttcctaa ccgaacgaat gtgcaattct tggaagtaat cgatagacag
    27001 accatcagga tagaaatctg ggaacgtggc gcgggttata ccctggcaag cggatcgtct
    27061 tcctgtgcgg ctgcggcagc ggctagaaga ctcggtcttg tcgagaatga ggtaagagta
    27121 agaatggctg gtggaacttt atatatcgat atgcgcgagc caaatataaa aatgaccggt
    27181 ccagccgagc actctttcga tctggtattt tcgccttccc taatgcgcct tgccatgaaa
    27241 gataaagggg attgatatgg ctcaatggaa aaatctggca tttctgcaaa aagaagtgtt
    27301 tttcgaatct agagccatgg aagaatggct catagaaatg cgcaggtcta tacaccggat
    27361 tccagaaccg gggaataagg aacaaaagac atcgagtttc atctgtaaga tgctcgaaga
    27421 aatggatata cctcatagaa gctatggaac agccgttgtt ggccttatcg aagggaaaga
    27481 gccaggacgc tgcgttgcct tgcgtgcgga tatggatgct ctgcccctag aggaaccttc
    27541 tgaccggccc tatgcctcgc tccaccatgg ttatatgcat gcctgcggcc atgatgccca
    27601 tatgaccgtg gcgctcggtg tagcaagatt acttgcggct cacaggcggg atttctcagg
    27661 ctcggtgaaa tttctttttc aacccgcaga agagactacc ggtggtgcga aggccatgat
    27721 tgaggctggc tgtctcgaga atccgcatgt cgatctggtg ctgggcctcc acgttgtgcc
    27781 tgaacttatg gctggcatgg tagaagtcaa gattggtccc ttctatggaa catccgacag
    27841 cctggagatc attgtcaaag gcagaagttc ccatgctgct tatccggaac aaggcgtaga
    27901 tgctattgta gtttcagcat ctatcattca agccctccaa accatagtct ctcgctctat
    27961 ttcagccctc gataatgcag tccttacaat tggcaagatt cagggaggag tgcgcagcaa
    28021 cattctcgca aacgaagtca tcatgattgg tacaattagg gctctttcag aagatgtcca
    28081 atgctttatg cgtaaaagag tcaaagaaat ctgcacagaa atcgccgcag cctttggagc
    28141 ttcctgcgag gtccatattc gtccgagcta tcctgttatg ataaatgacc agagcgcgac
    28201 agagctcgtg cgcgatactg ccatatccat tctgggagag tccaatgtga ttatgcgcca
    28261 acagcctaat cttggcgttg aagactttgc atattttctt agagagaggc cggggacttt
    28321 ttggcatctg ggctgtggaa agccgggaat ggagaaaatc cacccgcttc attccgctga
    28381 attcgatata gatgaatcgt gtctgcccat aggtgtcgca attcaagcgg cttcagcgct
    28441 gacttttctg catttgcgta atcaggaatc atgcggataa gtgtataact aggaattnnn
    28501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnact caagaataat tcaggaatag
    28621 ctcaaggatg actcaacgac gagtcgccga taaagatccc tttgtagcct ctatcaaagc
    28681 ggaaaaaggc gcaaggaatg caccatagcg aagaaaaagc ccttcaggat gaaactgcag
    28741 tgctagagcg aatcgttcag gatctattgg ttccaatgct tcgattatgc catctgagct
    28801 gcgcgccgtg attctaaaat cgctcgcggt tttgtccact gcctggtgat gaaaactatt
    28861 gaccaggagc ctttcggttt tgaaaagctc gaacagaacg ctttcttttt cgatggctat
    28921 gtgatggaac aactcatcgt aagctatctg cggattgtgt aaaagcgccg cgggattgct
    28981 gcgctcgata tcctgcaaga ggcttccgcc acgggctaca ttcataagct gacaacccct
    29041 acaaatcccc agcatgggta ttcttgcttt ccatgcttga ttgcagagct cgatctccca
    29101 ttcgtctcgc agggtatcga aagaaccaag gcctaaaatt ggttcttggt caaaaagcca
    29161 aggtgcaata tcgcctccgc ctgaaaagat cagaccatcc agaatagaag tgagcgcctt
    29221 cataacagct gaactcgtaa cagctagaat caaaggcgtg ccgccagcca tctctattgc
    29281 tttgacataa gattctttga gagaaaaata ggccactggt tgccgagacc gatccacaga
    29341 gctcgtaata cctataagag gctttttcat acagttattg tagtcaatcc ggtttgccag
    29401 gcatagtgga ggctcaaagt gctagcataa tcagaaaagg gtcagtaaag tggaaatgga
    29461 ggaagcaatg aaagatacgc ctttttcccc cgctattctt atcgatggct ctaagtgcta
    29521 tttcgacggt gtcgatctta gatatctggc tcatgagttt ggcactccgc tatatgtgat
    29581 gtccgaaacc attatccgag aacgctgtga agagcttcgt agtgcttttc tttgccgcta
    29641 cccaggaact cgagcctatt acgcaagcaa ggcgttgcag actctcgatg tgctcaagat
    29701 aataaaagaa gaaggtcttg gagtcgatgt cgtatcggga ggagagctcc atgcggcact
    29761 gaatgctgga gttccttcct ccgatatcat atttcatggg aatgcaaaaa cagacgagga
    29821 gctacattat gccattagct ctaaggtggg actgatcgcc gtcgataaca tcgaggagat
    29881 ttttaggatc gaggaggagg cagatagact cagtagagat cagcgggtaa ttcttcgcgt
    29941 ttcgcctgga gtagacagtc acacccatcg attcatttcc accggaagcc tcgattctaa
    30001 gtttggcctt ccaattgagc attcggaagt tgagcgatat gtaaaggctc tcgctctgtg
    30061 ccctcatgtc gaattctcgg gcttacattt ccatgtcggc tcgcaactcc tagaaaatac
    30121 ttcgcatctg gcagcgcttg aaatagtatt gaattttgcc gcgaaacttt cgaaggaatt
    30181 cggacttcag gtgaaggaga taaattttgg aggaggcttt ggtattcgct atctcccttc
    30241 cgatagggcg cccgctcttc ttgattttat tgatcccatg atgtcgatgg taaaagagtg
    30301 gagtcggaaa gaaaatctac ccatgccggt ctgcgcaatc gaaccaggac gatggattgt
    30361 aggagaagcc ggaataacgc tctataaagt tattgctgtc aaagaaatac ctggtgtgcg
    30421 cacctatgtc tccgtagatg gtggaatggg cgataatatc aggcctgcac tctatgatgc
    30481 tcgataccat gtagaaacgg tgagccaggc ttgtgcaatg gttccttgcg agaaggagaa
    30541 gaattgctcc aaggtcgagg ttttaaggga gagagatgct gaaaaagcct taggtatgga
    30601 ctcgaataga gagccaatct tgcgaaaggt gacgattgcg ggtcgctact gtgagtcagg
    30661 tgacataatt gcagaagatg tattcttgcc tgatcccaag ccgctcgata tccttgctgt
    30721 ctattcgaca ggcgcctatt gtttttcaat gtcgagcaat tacaacagag tgccgcgtcc
    30781 cgccatggta attacaaaaa gtggggatta tcggctgtcg gtaaggcgcg aaacatacga
    30841 tgatctattg agaagagaaa tctgaaaggc ataactaatg ttgtggcaga aaaagttgcg
    30901 caattatatc tgcttggctt agagataatg gaaatattac ggcattcctg tacaacgctg
    30961 tttataatga gcccatctgt ccagacagat gggctcatta tatcgagttg taagggtact
    31021 ttttcgcatc gagctcaagc tcttgagcga tagagaa
//
LOCUS       DAOMZL010000012        30885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_1143_length_30885_cov_15.249108, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000012 DAOMZL010000000
VERSION     DAOMZL010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 30885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30885
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1143_length_30885_cov_15.249108"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1339
                     /locus_tag="PLQ30_02030"
     CDS             188..1339
                     /locus_tag="PLQ30_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HPW01251.1"
                     /translation="MPPRGDQWSRTAARIFRTIWRNPSISRIDLAHKLDLDKSTVTKQ
                     VAHLIENGLIVEQEEGEASARGGRKPICLSANKEYGHFIGIELQLGFYDAVIVDLSGN
                     ILATLRGDTHVTSDNFVQVVLQIIEETRKQRCNSSLLLGVGVGTAGLIDSKKGRIRYS
                     VPLGIDRTLDFAKAIEGKLDVPCFIENDANCCAWGELAFNRVEELKDFLFALVEYRKD
                     ETSLLKYGGIGVGFGIVLGGKVHTGAHGYTGEFRSAFCNGPGDLQFSLTRNELMLLRF
                     DRGLLNRAVDELARNMAMLINTMDFQQVFIGGNIEALDVDLPTVLRRRLKENWMYPSP
                     KDVKIRYSSFGDKAVAFGAAGMVFERLLSEQRLPGLTSSFSSDNMGGIS"
     gene            1422..2726
                     /locus_tag="PLQ30_02035"
     CDS             1422..2726
                     /locus_tag="PLQ30_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPW01252.1"
                     /translation="MKKALFALLTIFVVLGAVVAQEKVTLNVLNYQQADQAGYQEDVA
                     IWEKFQKDNPDIKLNIEVLFSEPYHQKLQVYAAAGTLPDVFYVWPTSRSAIIHEKKLA
                     KDLSKLLGPDFLKDFSGAATDPNNQLGKYLAMLPQAFTYTSVMFVNKKLLADNGFDLP
                     KTYDDLKRMVWKLKAKGINVIMLPNKDGWPMQSCLFSTVLGRMAGDDFVDAILAGKAK
                     FTDKPFVDSLKVIDNLYKDGIMAREDNQVGYGEGPGLFVSGKAAIYIDGDWRAGAYIT
                     DKASGKALIPPASQEKDFVLLPFPSIPGEVNPGVVSGIAGTGWAISAAIPSGSAREKA
                     AVKLFKYLYSEEVQSIRYSTGSYVPTRKNVKANVEPLVAKVPQFYADNPKVCYVFDGL
                     FDPAVYNVLNDGLIAIGLGTATPEKVAADVQKAFETWKASSK"
     gene            2822..3946
                     /locus_tag="PLQ30_02040"
     CDS             2822..3946
                     /locus_tag="PLQ30_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPW01253.1"
                     /translation="MAFSSKSYIEEKRAYWAMVMPAFGIYLLVMAFPIVLSIVLSVSN
                     YSGGKMFGGEKWGFAGFDAYLRVFRDPWFWNALKNNIYIVLISVFGQLPLGFIFAYFI
                     YAKIVKAPEFWQGVLYVPNIISVIVVGLLWQTIFSPHGPIAEVINSIRVREFQSQLRG
                     IFDAAGGFSLSDDVVKRILDLVGPSGQAMFSDPLVDLRDFLSSFHGEGIKEVYNALSN
                     LFVQKWSPAFLTKPNIAMLPILFVILWMYTGMYLILFLANMQKIDVQVVESARIDGAN
                     EGQVMRYIILPALSGTIVNAAILAISGSLSSFALIFAMTGGGPSRVTEILSIYMYNNA
                     FLGRPNFPLANAISLIMVLISFGLIALTKAVEKRYGGKEE"
     gene            3959..4810
                     /locus_tag="PLQ30_02045"
     CDS             3959..4810
                     /locus_tag="PLQ30_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPW01254.1"
                     /translation="MIKESKCKNILSIIGKAAVYLIMIIFTFMTLYPIFWLIMNSFKT
                     TREFQISQLALPKSPTLQNYKGAWEIGDFSLLFPNSILYTLGATLGIIFLSLLAGFAF
                     AKLKSKATKPIYNSFIMGILLTTQTLMIPLFLEVNLLGIYNTRFAVLLVYIGAGLPIG
                     IYLSTEYIRAIPSAVVESARIDGAGFFRIFLSIIMPMSVPVATTLAILNITNTWNEFA
                     LINILVSKTELKSLPLGIYKFSGSLSSDYGKQFAALTIGMIPMLVFYMIFRKQITKGV
                     AAGAIKG"
     gene            4836..6176
                     /locus_tag="PLQ30_02050"
     CDS             4836..6176
                     /locus_tag="PLQ30_02050"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HPW01255.1"
                     /translation="MNREDGFPQDFIWGCSTSCYQIEGAAKEDGKGISIWDTFAHTPD
                     KVLSNHTGDIACDSYHRWKEDIALLRELNAGAYRFSVSWTRIQPEGKGAPNKKGLDYY
                     SRLVDGLCEAGVEPWLELFHWDLPQALEEKGGWRNRDTAMRFEEYAGIMYDHIGDRVG
                     HWTSMNEPWCAAFLGHLTGIHAPGLKDRSATNRAVHHLLLAHGLALRAFRRSGIKGEY
                     GLVINPSKPRPATLRPEDAEASERASIERTSLWLDPIFGRSYPRVFMDIFGSDMPIEA
                     GDMDIISEPLDFIGVNYYDEEAVRAAPCSAQNPYGFEFVPTYQKKTDMGWEIEPSGLR
                     RILSYIANVWPCKKIYVTENGAAFPDKQDADGIVRDYDRIDYIKGHANACGDAIKQGV
                     PLKGYFLWSLIDNFEWSFGYNRAFGIVAVDRNTLERRPKLSFYYYRDLIAGFGL"
     gene            6187..7794
                     /locus_tag="PLQ30_02055"
     CDS             6187..7794
                     /locus_tag="PLQ30_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HPW01256.1"
                     /translation="MSDRYSIIPIPVAYESREGNFRFDKETWLIAKPGLERETEVLAA
                     WLSRVPGIEKVKIVSANSNISESLFMPSGHERNKPEGSMILLSLEPELFFNTPEGYIL
                     DIAPSCIQIKAAESAGIIRGAASLWQLALGNGRKEEQGVSIQAGKIVDEPRFGWRGLM
                     LDCARNFFRVEFIEKLLDLAAFHKLNVFHWHLTDDQAWRLEIPSKPELVQYGAFRRDL
                     RYQEEYKKGGYYSQSDVKRVIDYAQARHILVVPEIELPGHSIALLASHPEYSCKGRAD
                     PTVHFEPEDRYGIFEDIVCAGNDDVFVYFDDVLQEVCKMFPGPYVHMGGDEAPKTRWL
                     ECSDCRERMNTLGFVSEDSGYLPEKLQAWFMGKISNLLFKYGRRMIGWDEVIDGDIRR
                     DTIVMSWRGIEPGIHAAKRGYDVIMCPQTRACYLDHKHNDEPEEPGHLGVCTLSDSYL
                     FEPVSDKLSDEESDHILGGQANLWSELIYFGRQAEYMLFPRLCALSEVFWSPREKRSF
                     EDFSQRLRVHEHRLDALNVCYYKGRFF"
     gene            8055..10253
                     /locus_tag="PLQ30_02060"
     CDS             8055..10253
                     /locus_tag="PLQ30_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW01257.1"
                     /translation="MRTILHFLKPYWLNIVLIILFLFIQANADLSLPDYLSRIVNVGI
                     QQSGIEADLPAVMRKSTFEALQKLLQMEARPEDFTALQEVYIEVLPDSPKAQSLSKKW
                     PIAKKESIMMLSSEDKAELDAARKVFLSEYPKLAAFLQFSSMAASKASPQMSSSDLSG
                     LSSMTPQELSAQIEALDPIIKSQIIVLGLQGEFTAMGANIGAMQRSYILRVGIQMLLI
                     TLITVGSAVSVGFLGSRLSAGTSRDLRNALFSKVEEFSLAEFDSFSTASLITRSTNDI
                     QQIQMLIMMSTRMLFYAPIIGIGGIIRALGKASGMWWIIAMAVGALLAIIGLVFALVV
                     PKFRIVQKLMDRLNLVVRENLSGMMVIRSFNMQDQEAKRFDKANRDLTDMMLYVTRAM
                     VVLMPLMTIIMNVVSISIIWVGSHEVAVGSIRIGDMMAFMQYSMQIFFAFLMMSAMFI
                     LFPRASVSAERVAEVLDKEVSIRDPEKPVHLSKPVKGEIEFHQVDFKYPGAQEQVLED
                     ISFIAKPGTMTAIIGTTGSGKSTLVSLIPRLYDVTGGQILLDGIDIRNLPLSELRAQI
                     GFVPQKSILFSGDIEENIRYGKENALENEIMEALEVSQVLSLVEESPEGLKRPISQGG
                     MNVSGGQRQRLAIARALVRKAPIYIFDDSFSAIDYKTDRAIRSALKRYAADSTIFLVT
                     QRVAPIRHAEQIIVLHEGQIVGIGMHEQLMKTCTVYRDIALSQLKQEELV"
     gene            10250..12196
                     /locus_tag="PLQ30_02065"
     CDS             10250..12196
                     /locus_tag="PLQ30_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW01258.1"
                     /translation="MNTNDKQENFDNSSDFPDTQMKRRQRTKPVPPPGRRGLMAGEKS
                     RDFKGTMKKLIAYLAPYRWPILFAIIFAIVSTILAILGPKVLGNATTTLFEGVVKKTT
                     RTGDIDFAKIKTILLTTLALYLSSAILQYFQGWVLSGIAVKITYSFRKDILAKINRMP
                     FRAFDSTNHGEILSRITNDVDVVNQTLTQTISQMIHSIMAIIGVFVMMLTISWQMTII
                     ALVTIPLSLIVVRVIVKRSQKYFRIQQEYLGHVNSHIEEIFSSHIVVKAYGGEQKAVQ
                     TFNNLNSTLYRSAWKSHFLSSIMMPLMNFVGNIGYVAVAVSGGWLAAKKMVTVGDIQA
                     FLQYVRQFTQPIVQIANISNVLQQTAAAAERIFDFLASEEENVSVESPQNGALVEIPK
                     SIKGKVEFRHVRFGYLPGQPIIKDFNAMVLPGQKVAIVGPTGAGKTTLVKLLMRFYDP
                     DSGEILIDDIPIGNLPRSVVRSWFGMVLQDTWLFSGTVRNNIAYGKRDATDAEIIEAA
                     KAAHVDHFVRALPEGYDFEINEETTNISQGQMQLLTIARAMLAQPPMLILDEATSNVD
                     THTEVLIQRAMGVLMQGRTSFVIAHRLSTIRDADLILVMDHGDIVEQGTHQELLSKGG
                     FYAELYNSQFDQNGEDQLANASGL"
     gene            complement(12288..13925)
                     /gene="groL"
                     /locus_tag="PLQ30_02070"
     CDS             complement(12288..13925)
                     /gene="groL"
                     /locus_tag="PLQ30_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710555.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPW01259.1"
                     /translation="MAKQLLFSEDARRKLLVGVETISEAVKVTLGPKGRNVLLDKKFG
                     APTVTKDGVSVAKEIELEDPYENMGAQLLKEVATKTNDIAGDGTTTATVLAYSIVKEG
                     LKSVAAGIDPMALKRGIDQAVTFSVEEIRKISKEIKEKEEIAHVASVSANNDIDIGNQ
                     IADAMEKVGKDGVITVEESKTMETTIEYVEGMQFDRGYTSPYFVTNRDSMTTVFENPL
                     ILIHDKKISNMKDLLPILEKIAQTGKPLLIIAEEVEGEALATLIVNHLRGTLNVCAVK
                     APGFGDRRKAMLEDIAILTGGEVISEELGLKLENTSISQLGTAKTVKVDKDNTTIING
                     GGKQKDIQDRIAQIKMQIEETTSDYDREKLQERLAKLAGGVAVINVGAATEIEMKEKK
                     HRVEDALSATRAAIEEGIVPGGGIALIQAALALDKADTSKMADDERVGFKIVKRALEE
                     PIRQIAENAGIDGSIIADKAKQEKKGIGFDAAKLEWTDMTKAGIIDPAKVTRYALQNA
                     ASVAALLLTTECAITDLPEKNPPAMPSPNAGMGGMEY"
     gene            14189..14920
                     /locus_tag="PLQ30_02075"
     CDS             14189..14920
                     /locus_tag="PLQ30_02075"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPW01260.1"
                     /translation="MKESCAILYADQDLLAIDKPAGILAIPDRWDPNIPVAQQLLQSQ
                     FGTLLPVHRIDKDTTGVLLYARNEVAHRIISSDFSSRKIEKIYFAIVHGQPESQAWEV
                     GLPLRADGDRLHRTIIDASKGKEAKTRFEVIERFRGFALVLAIPETGRTHQIRVHLAA
                     SGLAIVADPLYGNGDPLLLSMIKPGWKGDAYKERPLIARTALHSAKVSFIHPKTKKEL
                     LIEAPFPRDFKATLAQLRKVRPSWV"
     gene            complement(14935..16305)
                     /locus_tag="PLQ30_02080"
     CDS             complement(14935..16305)
                     /locus_tag="PLQ30_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW01261.1"
                     /translation="MPLQKSDLSDAVINTYYAVRGPIVARAQQLEQAGKEIIYCNIGN
                     PQALKQKPLTYVRNTLAACEQPGLLNISDNFFMEDIKEKARHILEQSKHGLGAYSESK
                     GIRFVRNAISDFIKSRDSFDGVEQTADPEHIYLTDGASKGVQAALTLLIAEKNDGILI
                     PIPQYPLYSATITLYNGQQIGYYLDEETGWSLDERMLEDAIQEALAKGIRARAIVVIN
                     PGNPTGGVLTEDNIRMVIRFAKRHNITILADEVYQENIYKPGARFISFAHVMTSMREQ
                     EVSLFSFHSVSKGFFGECGHRGGYMEVRNLPCEVIDQITKLQSISLCANLPGQILTYL
                     MVSPPKPGDPSFQQFANERNAILTELAKRAKMLQEGLNSIPGYRCQPIDGAMYAFPSI
                     TLPPGKSDDDYCMALLEQTGVCVVAGSGFGQKPGTAHFRTTILPPTEQIERVVDALGK
                     FHRTWK"
     gene            16425..17732
                     /gene="hflX"
                     /locus_tag="PLQ30_02085"
     CDS             16425..17732
                     /gene="hflX"
                     /locus_tag="PLQ30_02085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPW01262.1"
                     /translation="MTGVKRGEQETSRVFLIGVASSEIPRSEAQELLDELHRLTDTLG
                     NEVAGEMLVLLRESTPSLLIGTGKAQEIVDAADQAGADTIIFDHVLSPVQQRNWEKLS
                     SKNVYDRAELIIKIFASRAFTKEASLQVELAQLEYALPRLAHSYNDLMQQRGGRYGTK
                     GSGEQKLELDRREIERRIHEIKGELETVRKERAVQRKRRERSAVPRAAIVGYTNTGKS
                     SLLNELTLASVHAEDKLFATLDPTTRRIQLVRGQTLLLTDTVGFIRNLPPGLVEAFHS
                     TLEEVSLADVLIHVADASDKSIDKHIETTIKVLFDIGAGEIPHILVLNKIDLVDPVVV
                     QAFLSRYPGSIAVSAKTQEGLGELVKEIQEALTKQMETLMLRIPHADNHVVSLILREA
                     TVLDQYYDEEYTWIRCRIPRKLIRAVQKYAYKGALPLMDSIKP"
     gene            17764..18702
                     /locus_tag="PLQ30_02090"
     CDS             17764..18702
                     /locus_tag="PLQ30_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HPW01263.1"
                     /translation="MKNIVSVVVVGAGAIGASIAVRLMEAGKQVVISANGERKARYLK
                     QGFVVNGRQYFLPVKDKYEADPADLVIIAVKNYSLEEAIEEMRPYVSVNTIILSLLNG
                     IDAVPRLRREFGEDSVPYAMILGIDALREENRVQYLAKGRIFCGFEKEKVEKNASTLA
                     VLEEFFRTSDIAFVVPEDIVKEIWFKFMINVGLNQWSALIRAPYRLFQDSPHGQELLS
                     KTMIEVISLSKRYGGNLEETDIDRAIAVLKTLAPQGKTSMLQDVEARRQTEVDAFAGA
                     MKRLAKAADLDVPINAILYDAIRAIEDSFEDSYKAE"
     gene            complement(18685..19503)
                     /locus_tag="PLQ30_02095"
     CDS             complement(18685..19503)
                     /locus_tag="PLQ30_02095"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPW01264.1"
                     /translation="MRILSYKSIDSTMDEARRILQEGLVKHITIVRADTQTAGRGRIE
                     GRAWIDMPEHCLLMTIILPQSYKDISAIPLRAGLGVVRALETSLKDPALETQPFPELM
                     DNYGQHEHKLAPYFLLKWPNDIVTHIPGDSNCFGKLCGILCEQTADTVLIGIGLNLLK
                     SRYSGLNTDLSPLSLEEALGFIPRAFANLDDAACSITEHVIQALHDPTWHSEYEHRLW
                     GRGALMRFIIGHPRLAQEVEGICVGTSEDGSLILSNNGKKRKFVSGEISLLRFV"
     gene            19670..21502
                     /gene="glmS"
                     /locus_tag="PLQ30_02100"
     CDS             19670..21502
                     /gene="glmS"
                     /locus_tag="PLQ30_02100"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPW01265.1"
                     /translation="MCGIIGYSGPRKASKVLLEGLRRLEYRGYDSAGIAVGRTDPKPN
                     LQIIKAVGKIGELAKKIPEDLDGQWGIGHTRWATHGGVTEANAHPHTDISGKIAVVHN
                     GIIENHKTLRTVLEKKGYVFKSETDTEVIPHLIASYYEGDLLTAVLAALQHLEGTYGI
                     ACIHADEPGRIVGARNGSPLIVGVGKDEMFLASDFTAMVAYTNRVIYLNDGEVVDITQ
                     ENYSITDRHSNSIAKKVDEITWELGAMEKSGFMHYMEKEIFEQPDSIARAMSGRIDEE
                     NATAKLGGLNLTRRQLAEVHRVRIIAAGTSWHAGIAGSYLLEQVARIPAQAELASELR
                     YRNPVVEQGSLWFVVSQSGETADSLFAMREIQRKGATVLGICNVVGSTIPRESDGGVY
                     VHSGPEIAVASTKAFTSQLTVFFLFTLLMARMRDMSREEGKIFIRALTGVPDMVRDVL
                     SKRDRIQAIAKKYCRTKDFLYLGRGILYPIALEGALKLKEISYIHAEGYGAGEMKHGP
                     IALISPEVPSVFLVSDDYLHDKTISNMREIKARGGPVIAICSDEDKEAMSFADDFISV
                     KKADPRFYPFSMIIPLQLFAYFCALELGCNVDQPRNLAKSVTVE"
     gene            21499..22518
                     /locus_tag="PLQ30_02105"
     CDS             21499..22518
                     /locus_tag="PLQ30_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPW01266.1"
                     /translation="MKHGAWRVLPIVVFAIALVGALAFNVALVSRSRNALASTILRNP
                     STIHSARFHVIAILPDTVDPFFMHLKEGLSEEAEAQDAALQIFYFSPTGGADSGGISG
                     EVLRWFEIALRSKTDGIILFKTAGLDVDRLMEAAESAQIPFVPLAMDVSQEWINIGVT
                     GDSASQGREASALALGMLGAAARIGIILSSDTSLGYAFYEEPFYRGVEETLKSKQGAA
                     IVAAEREEESILGGEDACARMLAEHPDINAILCIDAKATIGAAQVVIDRGMVGQIVII
                     GADENSEVNRLIEKGVVQASIVRDAVAMGKAGVALAIGQRIGIRAPERISVGYRVIPA
                     LGGGL"
     gene            22515..24026
                     /locus_tag="PLQ30_02110"
     CDS             22515..24026
                     /locus_tag="PLQ30_02110"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPW01267.1"
                     /translation="MIDFTWLSRPLRARRSRTGLRDRIIANSAVILLVLVIATSYTAS
                     TSFNLARSVELLFHNSVSMQELRTVLRATQENLMGYLSAKNSESLREFIRNSTLLSNM
                     AHKLNKENKNDDVFLLEKDLAYLVESYLASAERAVQAKRGRDVTEYVSYYEEARRGAS
                     YIEFLSTRMDTIYLGQSLTGFSSYRANISLAIMSNGVLLLAAFLLSIALIARYSYTIT
                     EPLARLSVAAGAVGRGEYNHPLPPYERKDEIRTLHDAFVNMQDSVQKAFIELTRKAEL
                     ERSLMEERMRVLTYQHRLKDAELLALQTQINPHFLYNTLAAGWQIALSEGNEKTAEFL
                     EKLAAFIRYSLKPSTRFVLVSEEIECVRQYIWLLRLRFGERFEFCLDIQDEALGCETP
                     ALVLQPLVENAIVHGLKDIEEGGRVLISAKLEGDTIHFSVSDTGCGMSNECIALALAA
                     ASPEDTRPQNGIGLHNVVRRVALATGGQGRVEIESELGNGTCVTILLPAGKIS"
     gene            24023..25663
                     /locus_tag="PLQ30_02115"
     CDS             24023..25663
                     /locus_tag="PLQ30_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW01268.1"
                     /translation="MKRVLIADDEIPIVNGLQLLFKRYFQDGYLVVGTAQSGREVIEK
                     SKTLAPDIVLMDVHMPGINGLDAIRELSQQGGARAFILVTAYERFDIAREALSMGVCD
                     YLLKPVSKERLEIALQAASSYLDRLHILEEKELEFKERQQTLLPFIKTAFFVYLRQQR
                     EAKDKEPRDPKRTKALKEELNLYKEVLQISGETGVMGIASFSPADGLANSLYEHFCST
                     LQYKTLALAGPLEDNRYCAWFLPLKSGIDQGTVAEIEITAFLELLGSSFSSQLATGEL
                     RFACGQPELLENIGRSWDNAVRNFGLGLESINRYRPRLGEDIAENREEPASVFDTQFY
                     DAIMEGQYAMAGQLLEKLILTVEETQDKPRDLYFRIIAALSFWSSKLAGGGVLSDNVY
                     RDFMDFGDIEKLWEERFWHLFASAVRGRFQSLQQHAAEVGTHSPFVVKALQYIENHYQ
                     EPITLESASAAIGISAGHLTRLMSDELKRGFARTLIGYRMQKAKELLKMPNISIREVS
                     QRCGYSDANYFARLFRRLTGLTPREYAARVTKGEDSDA"
     gene            25656..26711
                     /locus_tag="PLQ30_02120"
     CDS             25656..26711
                     /locus_tag="PLQ30_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPW01269.1"
                     /translation="MPEAMRKASGPIFFLIAMILATTALGLLSCSKSEKTSETKSPLI
                     GFSLDSLVVERWRRDVDSFTKAAHDLGAEVVLRVANQNAETQIAQVRELLSQGIDVLV
                     IIPNDAERLSEVCREAKRKGVQVLSYDRLVHRANVDLYISFDNEKVGSLQAQAVVEVV
                     PQGNYVIINGATTDNNAFMINKGFHSVLDPFIESGKIQLMEEIWPSDWMSDEVRTQFE
                     ALVKPGQKIDAVLCGNDMLAETVISVLSENRMVGKTKVTGQDAELSACQRIAEGSQFA
                     TVYKPIDSLALKAAGFAVMMAKGEKIGYENKIEDGTYKVPYIALEPVLVTAKTLESTV
                     IEDGFHSRDDVYRNVKH"
     gene            26952..28034
                     /gene="chvE"
                     /locus_tag="PLQ30_02125"
     CDS             26952..28034
                     /gene="chvE"
                     /locus_tag="PLQ30_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPW01270.1"
                     /translation="MKRTLLIAMLFAVVAGFVFAETQVGIVLPTKDEPRWIQDQTQFL
                     DALKTAGFSAKVLFSQGDSAKEKANVEALISEGIKVLIICPHDGTAAASAADLAHSAG
                     VKVISYDRLIRDTTSVDYYVTFDSFEVGKAWGDYLNSKVPAGTKRNNLYLYAGAASDN
                     NSFIFFEGAWSALQPKIADGTYIVRNSDKAAALAKKAKLTRDEAAQIIGQVTTNWNFS
                     DAKNKAEANLTAAPKEAKGTVYICAPNDGTARAIADAFAADKDVKTYYITGQDAEIAS
                     IQYIIDGKQSMTVLKDVRTLVKDAISAATAYMKGQTPPVTAYYNNGKKDVPAKPTAIV
                     TVTKENVKKEIIDSGYWPADKFTGLK"
     gene            28136..29659
                     /gene="gguA"
                     /locus_tag="PLQ30_02130"
     CDS             28136..29659
                     /gene="gguA"
                     /locus_tag="PLQ30_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPW01271.1"
                     /translation="MNDNILEMRHITKTFPGVRALNDVSFAVRRGEIHCLVGENGAGK
                     STLMKVLSGLYPHNEFEGEIVLNGKKVEFNGIHDSEEAGVGIIYQELALVPEMTVYEN
                     LMLGHEIRRGMTIDVHEMIRKAEDLLKRVRLSINPTAKIKDLGIGKKQLVVIAKALSR
                     NVSLLILDEPTATLNEDDCENLLDIIRMLKMQGVTSIMISHKLKEVLSIAETITVLRD
                     GQTICTLDRRMDDVNEQVLIRHMVGRSIENIYPPRISKPEETISIEVKNWNAYDPKLG
                     RKVLNNVNFYVRKGEVVGLAGLVGSGRTELARSLFGNPDKYEISGDLILKGEKRRFSH
                     PSQAIKAGLAYATEDRKANGLILIKSIQENISLANLQEVSKNGLINEELDKQTALSFV
                     ESLSIKTPGIHQLVMNLSGGNQQKVSVAKWLFVGPDVLIFDEPTRGIDVGAKYEIYNI
                     INDLVAKGMSILMISSELPEILGMSDRIYVVANGTITGELDAKSATQEAIMRLATQY"
     gene            29671..30849
                     /gene="gguB"
                     /locus_tag="PLQ30_02135"
     CDS             29671..30849
                     /gene="gguB"
                     /locus_tag="PLQ30_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPW01272.1"
                     /translation="MSEFMTVVKKNVREYGMYIALAAIMAVFTILSKGIFISSRNIAN
                     LLNQTGYIAVLAVGMTLVIVIRHIDLSVGFLSGFLGAIAAIALTQWNWSLLPTILFVL
                     ALGVVAGLFTALPVASLGIPAFVASLAGWLIYRGALMLVTAGTGTIIIPNEAFNAIGN
                     GYIPDILASETFLPGVHKTTLLLGAIAIVFAIFSQIADRKRKIQYKFEVSSSGLFAAQ
                     LIFVSVILGFITWILAGYNGLSWTIVIMLAVVLVYDFITRRTVLGRHIYAVGGNPEAA
                     ELSGIRVKKITFVVFASMGFLAALSGILFTARLKSATPQAGTLFEMDAIASAYIGGVS
                     AAGGVGSISGSLIGALVYMSLMNGMNLLGTDISLQYIIRGLVLLLAVIFDVMTRKQKA
                     "
BASE COUNT         8300 a         6666 c         7568 g         8351 t
ORIGIN      
        1 cactttttgc ctcccaaaag aattctcctc acgccaaatt ctgaaagatt tttgttgaca
       61 attcaagata ccggtgttat atgttaattg gttggataaa tcaaccaaca attatgaaat
      121 tatgtcgaat ttacattttg ctggaagaaa aagcagaact tttaggctga cgttgaggag
      181 atagggcatg ccaccgcgtg gagatcaatg gagccgcaca gcggcaagga tttttcgcac
      241 catatggcgc aatccatcca taagtcgcat tgatcttgct cataagctgg atctcgataa
      301 atccacggta acaaagcaag tagcacatct tatcgaaaat ggcttgatag tggaacaaga
      361 agaaggagaa gcttcggctc gaggaggacg taaacctatc tgtctttcgg caaataaaga
      421 atatgggcat tttataggta tcgagcttca gctcggattc tatgatgcgg taatagtgga
      481 tctatcgggc aatattctgg ctactctacg gggcgatact catgtgacct ccgataattt
      541 tgttcaggta gtgctgcaaa taatcgaaga gacacgtaag caacgctgta atagttccct
      601 tcttctaggc gtcggagtag gtaccgcagg tctgatcgat tcgaagaagg gtcgcattag
      661 atactcggtt ccgctaggga tcgatagaac actggatttt gccaaagcca tcgaaggcaa
      721 attggatgtc ccttgcttta tcgagaatga tgcaaactgt tgtgcttggg gtgagctcgc
      781 tttcaataga gttgaagagc tcaaggattt tctctttgcg cttgttgaat atagaaagga
      841 tgagacttct cttttgaaat atggaggaat cggtgttgga tttgggatcg ttcttggagg
      901 gaaggtacat actggagccc atggttacac cggagaattc cgtagtgctt tctgcaatgg
      961 accgggagat ctgcaatttt ccttgactcg caacgagctc atgctgctga gattcgacag
     1021 gggtcttctc aatagagcag tggatgaact tgcacggaac atggccatgc tcataaacac
     1081 catggatttt cagcaagttt tcataggagg aaatatagaa gcgctcgatg tggatttgcc
     1141 tacggttttg cgccgaaggc tcaaagaaaa ctggatgtat ccttcaccaa aggatgtgaa
     1201 aattcgctat tcgagctttg gtgacaaggc ggtcgctttt ggcgctgcgg ggatggtttt
     1261 tgagcgctta ttgtctgaac agcgtctacc ggggctcact tcatcctttt cttcagacaa
     1321 tatgggcggt atttcttaat catatctaac aagggtaaaa aggcagatat gccttttcat
     1381 atcggaacac gggcagaatt gcccgacgta aggaggttct tatgaaaaaa gccttattcg
     1441 cgttactaac aattttcgtg gtgttaggcg cggttgtggc tcaggaaaag gttacactaa
     1501 atgtactcaa ctaccaacag gcagaccagg caggatatca ggaagatgtc gcgatctggg
     1561 agaaatttca gaaggataat ccggatatca aattgaatat cgaggttctt ttcagcgagc
     1621 catatcacca gaaactgcaa gtctatgcgg ctgctggcac tcttccggat gtattctatg
     1681 tatggcctac atctagaagt gcaattattc acgaaaaaaa gctcgctaaa gacctctcca
     1741 aactacttgg tccggatttc ctcaaggatt tttccggtgc ggctaccgat cccaataatc
     1801 agctaggaaa atatctggcc atgttgccac aggctttcac ctatacatct gtcatgtttg
     1861 tcaataagaa attgcttgcc gacaatggct tcgatttacc aaagacctac gacgatctga
     1921 agagaatggt atggaagcta aaggccaagg gtatcaatgt gataatgctg cctaataagg
     1981 atggctggcc gatgcaatct tgcctcttct ctactgtgct tgggcgcatg gctggcgatg
     2041 acttcgtcga tgccattctt gctggcaagg ccaagttcac ggacaagccc ttcgtggatt
     2101 cgctaaaggt catcgacaat ctatataaag atgggatcat ggcgcgcgaa gacaaccagg
     2161 tcggatatgg cgaaggaccc ggtctctttg tatccggaaa agcggcgatc tatatcgatg
     2221 gcgattggag agcaggcgct tacattacgg acaaggcttc aggcaaggct ctcatcccgc
     2281 cggcttcaca ggagaaagat tttgtacttc ttccgttccc aagtatcccg ggtgaagtga
     2341 accccggcgt tgtctctgga attgcaggta ctggctgggc aatatctgcg gctattcctt
     2401 caggatccgc aagggagaaa gcagcggtaa aactattcaa atatctctat tctgaggaag
     2461 tgcaatcaat tcgctatagc acgggatcat acgtgcctac gcgaaagaac gtaaaggcca
     2521 atgtcgaacc acttgttgca aaagtaccgc aattctatgc agataatccc aaggtatgct
     2581 atgtcttcga tggcttgttc gatccggccg tctataatgt gctgaatgat gggctaattg
     2641 ccatcggtct tggaacggca acgccggaga aagtagcagc cgatgttcag aaagccttcg
     2701 aaacctggaa agcttcatca aagtaagact attgaaaaag taggtgtcgg aaagcgtaga
     2761 cgccttagca tggaggagcc attgtgtctt tacgctttcc gctccttttt ggggagtcca
     2821 tatggccttt tctagcaaat cctatataga agaaaaacgg gcttactggg ctatggttat
     2881 gcccgccttt ggcatctatc tcttggtcat ggcattccct attgttcttt ctattgtgct
     2941 atcggtatcc aattattctg gcggcaagat gtttggaggc gaaaaatggg gattcgcagg
     3001 attcgatgcc tatctgcgtg tattccgtga tccctggttc tggaatgctc tcaagaataa
     3061 tatctatata gtgctcattt ctgtatttgg tcaacttccg ctgggattca tttttgctta
     3121 tttcatctat gccaaaatcg tcaaggcgcc tgaattctgg caaggtgtgc tttatgtgcc
     3181 caatattatt tctgtaatcg tggttggcct tttgtggcaa acgatttttt caccacatgg
     3241 gccaatagct gaggttataa attcgataag ggtcagagaa tttcaaagcc aacttagagg
     3301 aatatttgat gccgcgggtg ggttttccct ctcggatgat gtagtcaaga gaattctgga
     3361 tctcgtaggg ccttctgggc aagcgatgtt ttcggatccg cttgtggatc tccgcgattt
     3421 tctgtcttca ttccatggtg aaggaatcaa ggaggtctat aatgcgctca gcaatctttt
     3481 tgttcaaaaa tggagtcctg ccttcttgac aaagccaaat atcgcgatgc ttccgattct
     3541 ctttgttatt ctgtggatgt acacaggaat gtatctcatc ctttttttgg caaacatgca
     3601 aaagatcgat gtgcaggtcg tagagtcggc tcgcatcgat ggggcaaacg aagggcaggt
     3661 tatgcgctac atcattttgc ccgcattgtc ggggacaata gtcaatgccg cgattttagc
     3721 catttcaggc tcgctgtcca gttttgctct tatctttgcc atgactggcg gagggccttc
     3781 gcgtgtcacg gagatccttt ccatctatat gtataacaat gcgtttttgg gtaggccaaa
     3841 tttcccacta gccaacgcta tttcgctcat aatggtattg atcagttttg ggctgatcgc
     3901 tcttacaaaa gcagtagaaa agcgctatgg cggaaaggag gaatgagcaa tgcccgagat
     3961 gatcaaggaa tcgaaatgca agaatatcct atccataatt ggcaaagccg ctgtctatct
     4021 gattatgatc atttttacct ttatgacgct ctatccgatc ttctggctca ttatgaattc
     4081 gttcaagacg accagagaat ttcagataag ccagcttgca ctacccaaat ctcctaccct
     4141 ccagaattat aaaggagcat gggaaatagg agattttagc ctactatttc ccaattctat
     4201 cttgtatacg ctaggggcga ctcttggcat aatttttcta tcacttttgg caggttttgc
     4261 atttgccaaa ctcaagtcca aagcaacaaa gcctatctac aatagcttta tcatggggat
     4321 tcttctgact acccagacat tgatgatccc tctttttctt gaagtgaatc tgttgggtat
     4381 atataacact cgttttgcgg tacttcttgt atatattgga gccgggttgc caatcgggat
     4441 atatctgtcc acggagtata ttagagcaat tccaagcgcg gtagtcgaat cggctcgcat
     4501 agatggagct gggtttttca gaatattctt gagtattatt atgccaatga gtgtgcctgt
     4561 tgctacgact cttgcaattt tgaacattac aaatacatgg aatgaatttg cgctgattaa
     4621 tattttggtg tccaagactg agctcaaatc gcttcccctg ggtatctata aattttcggg
     4681 ttctctttct tccgattatg gaaagcaatt tgcggccctt acaatcggaa tgataccgat
     4741 gctcgtgttc tatatgattt tccgaaagca aattacaaag ggtgtggccg caggtgccat
     4801 aaaaggataa gtccctaatc aaaagaggag caagcatgaa tcgagaagat ggtttccctc
     4861 aggattttat atggggttgt tcgacctctt gctatcaaat tgaaggagcc gcaaaagaag
     4921 atggaaaagg gatctctata tgggatactt ttgcccatac gccagataaa gtgttaagca
     4981 atcatacagg agatatagcc tgcgattcct atcatagatg gaaagaagac atcgcacttc
     5041 tgagggagct caatgctgga gcttatcgct tttcggtttc ctggactcgc atccaaccgg
     5101 aagggaaggg tgcaccaaac aagaaaggcc tggattatta ttcgcgactt gtcgatggcc
     5161 tatgtgaggc aggtgtagag ccttggctcg agctgtttca ttgggatctg ccgcaagcat
     5221 tagaagagaa aggtggttgg cggaatcgcg atacagcgat gcgcttcgaa gaatatgcag
     5281 gcattatgta tgatcatatt ggagatagag ttggccattg gacaagcatg aatgagccat
     5341 ggtgcgctgc attcttaggc cacctaacag ggattcatgc gccaggctta aaagatcgta
     5401 gtgcgacaaa ccgagcagtg catcatctgc ttctagcgca tggacttgca ttaagagcat
     5461 ttagacggag cggaatcaag ggcgagtatg gtcttgtcat caatccttca aagccaaggc
     5521 cggcaactct ccggcctgaa gatgccgagg cttccgagcg agcttcgatt gagcgaactt
     5581 cgctttggct cgatcctatc tttggcaggt cttatcccag ggtttttatg gacatattcg
     5641 gctccgatat gccaattgag gcaggcgaca tggatataat atcagagcct ctcgatttta
     5701 ttggtgtaaa ttattatgat gaagaagctg ttcgcgcagc tccctgtagc gcccagaacc
     5761 cctatggatt tgaatttgtt cctacctatc agaagaagac cgatatggga tgggagatag
     5821 agccatcagg attgcgcaga atattatcat atatcgcaaa tgtatggccc tgcaaaaaga
     5881 tatatgttac cgagaatggc gcagcattcc cggataagca agatgctgat ggaattgtcc
     5941 gggattatga ccgaatagac tacataaaag gccacgccaa tgcctgtggg gatgctataa
     6001 agcaaggtgt tcctcttaaa ggctattttc tatggagttt gatcgacaat tttgaatggt
     6061 cctttggcta taatcgtgcc tttggcattg tagccgtaga tcgtaatacg cttgagagac
     6121 gccccaaact gagtttttat tattatcgcg atctcattgc aggctttggt ctctaaaggg
     6181 ggagagatgt ctgaccgtta cagtatcatt cctattcctg ttgcctatga gtccagagaa
     6241 ggcaatttcc gctttgataa agaaacatgg ttgattgcaa aacctgggct ggaaagagaa
     6301 acagaggtcc ttgcggcctg gctttccaga gttccaggca tagaaaaagt taaaatcgta
     6361 tctgcaaatt cgaatatttc tgagtctttg ttcatgccaa gtggacatga aagaaataag
     6421 cctgaaggtt cgatgatact gctctcacta gaaccggaat tatttttcaa tacgccggaa
     6481 ggctatattt tggatattgc gccatcctgc attcagatta aagcggcaga aagtgctggg
     6541 attatccgag gtgcggcaag tctatggcaa cttgccttag gaaatggccg caaagaagaa
     6601 caaggagtgt cgattcaagc cgggaaaatt gtagatgagc caagatttgg ctggcgaggc
     6661 ttgatgctcg attgtgcgcg caattttttt cgggtggagt tcatcgagaa acttctcgac
     6721 cttgcagcct tccacaagct caatgtattt cattggcacc ttacggacga tcaggcatgg
     6781 aggctcgaga ttccttcaaa acccgaactt gtgcagtatg gggcttttag gagagatctg
     6841 cgctaccaag aagaatataa aaaaggtgga tactatagcc aatccgatgt aaaacgcgtt
     6901 atagattatg cacaggccag gcacatcctt gtggtgccag agatagaatt gccaggtcat
     6961 agcatcgctc tcctcgcatc tcatccagaa tattcatgta aaggaagagc tgacccaact
     7021 gttcactttg aacctgaaga ccgctatgga attttcgagg atattgtttg tgccggaaat
     7081 gatgatgtgt ttgtctattt tgacgatgtg ctccaagagg tatgcaagat gttccctggt
     7141 ccatatgtac acatgggcgg agacgaggcg ccaaaaacca ggtggctgga atgttcagac
     7201 tgcagggagc gaatgaatac actgggcttc gtgagcgaag atagcgggta tttgcccgag
     7261 aaattgcagg catggttcat gggaaaaata tctaatctgc tcttcaagta cggcagaagg
     7321 atgattggct gggacgaggt catcgatggc gatatcagaa gagataccat cgtaatgtct
     7381 tggagaggaa tcgaaccggg tatccatgcg gctaagcgtg gttatgatgt gattatgtgt
     7441 cctcagacaa gagcttgcta tcttgatcat aagcataacg atgaaccaga agaacccggg
     7501 catcttggag tctgcacgct gagcgattcg tatctattcg agcccgtgtc ggacaaattg
     7561 agcgatgagg aatcagatca cattttgggt gggcaagcca atctatggag tgaactaata
     7621 tattttggca gacaggcgga gtatatgctg tttcctcgcc tctgcgccct ctcggaagta
     7681 ttttggagtc ctcgggaaaa acgcagtttt gaagacttta gccagcgctt gagggtgcat
     7741 gagcatagac tagatgcgct aaatgtctgt tattataaag gaagattctt ttaaaactcg
     7801 aactgtcggg ttaaatcaag ccttggtaat agaaaaaacc ctatcataca attcttcatt
     7861 tctccttgcc taaaacagga gcttttctta ttatttacac atcttttctt ttgaggcttt
     7921 gagaatatgt taaaaatctt ggataccaga tatgcttgtc tttttggtgg gttcaccaat
     7981 cccgaaaaag catcgattct ttcctttcct gcaataattt gaaactgatc atccgctttc
     8041 aataggagca caagatgcgg actattcttc attttctaaa accttattgg cttaatattg
     8101 ttttgattat cctctttcta tttattcaag cgaatgccga tctatccttg ccggattacc
     8161 tatcaagaat agtaaatgtg ggcattcaac aaagtggtat tgaggctgat ttacctgctg
     8221 tcatgcgaaa gagcactttt gaagcactgc aaaagcttct acagatggaa gccaggccag
     8281 aggattttac ggctctgcaa gaggtctata tcgaagtatt gccagattca cccaaagccc
     8341 aatcgctatc gaagaaatgg cctatcgcta agaaagagtc aatcatgatg ctttcaagcg
     8401 aggacaaagc tgagctcgat gcagctcgga aggtttttct gtcagaatat ccaaaacttg
     8461 ctgcgttttt gcagtttagt tcaatggcag cgagtaaggc atcgccacag atgtcgagtt
     8521 cggatctctc cggtctttct tcgatgactc ctcaagagct ttcggcacag atcgaagccc
     8581 tggatcctat tataaaatct cagattatag tccttgggct ccagggagaa ttcaccgcaa
     8641 tgggagccaa tatcggtgca atgcagcgtt cctatattct gcgtgttggt attcaaatgt
     8701 tgctcataac gcttatcact gtaggttctg cggtatctgt tggtttcctg ggttcgagat
     8761 tatctgcggg cacatcgaga gatctcagaa atgcgttatt ctcaaaagtc gaagaattct
     8821 cgcttgcaga attcgattca ttttccacag cctcgcttat cacgcgatct accaacgaca
     8881 ttcaacaaat ccaaatgctc ataatgatga gtacgcgtat gcttttttac gccccgatta
     8941 tcggaattgg aggcatcatt cgggctttgg ggaaggcctc tggaatgtgg tggatcatcg
     9001 cgatggctgt tggggctctt cttgccataa tcgggcttgt ttttgctctt gttgtcccca
     9061 aattccgaat agttcagaag cttatggata gactaaacct tgttgtgcgc gaaaacctat
     9121 caggtatgat ggtcattaga agcttcaata tgcaagatca ggaagcaaag cgctttgaca
     9181 aggctaatag ggacctgaca gatatgatgc tctatgtcac tagagctatg gtagtactca
     9241 tgcccctcat gaccatcatt atgaatgtgg tttcaatatc cattatttgg gtcggttccc
     9301 atgaggtagc cgtaggcagt attcgaatag gtgacatgat ggcttttatg caatactcaa
     9361 tgcagatttt ctttgcattc ctcatgatgt cagccatgtt tatccttttc ccaagggcga
     9421 gtgtctctgc ggaacgcgtt gcagaggttc tagataagga agtcagcatc cgtgatccgg
     9481 aaaaaccagt tcatctatcc aaaccagtca aaggggagat cgaattccat caagtcgatt
     9541 tcaaatatcc aggtgcgcaa gagcaggtgc tggaagatat cagctttatt gcaaagccag
     9601 gaacaatgac ggctattatt ggtacaacag ggtccggcaa gtccactctt gtcagcctaa
     9661 ttcctcgcct ctatgacgta actggaggac aaatactttt ggatgggata gacataagaa
     9721 atcttcccct ttcagagcta cgagctcaga taggttttgt tcctcagaag agtatacttt
     9781 tttctggcga cattgaagag aatattcgat atggaaaaga aaacgcgttg gaaaacgaaa
     9841 tcatggaggc tctcgaggtt tctcaggtcc tgagtcttgt ggaagaaagc ccggaagggc
     9901 taaagcggcc aatctctcaa ggtggtatga atgtttcagg aggacagcgc cagaggctcg
     9961 caatagcccg tgcgttggtc agaaaagcgc caatttacat cttcgatgat agtttttcgg
    10021 caatcgatta caagactgat cgtgctattc ggtcagcgct gaagagatat gcggcagaca
    10081 gcacgatttt tcttgtaacc cagcgcgttg cgcctattcg tcatgctgag cagataattg
    10141 tgcttcatga aggccagata gtaggaattg gtatgcatga acagcttatg aagacatgca
    10201 ctgtatatag agacatagcg ctttctcagc tcaaacaaga ggaactcgta tgaataccaa
    10261 tgataagcaa gaaaattttg ataattcatc tgattttcct gatacacaaa tgaagagaag
    10321 acagcgaaca aagccagttc caccacctgg tcgtagaggg ttgatggctg gcgaaaaatc
    10381 aagagatttc aagggcacaa tgaagaagct tattgcttat ctggctcctt atcgctggcc
    10441 aattctcttt gcgattattt ttgcgattgt atctacgatt cttgcaatac tcgggccaaa
    10501 ggttcttgga aatgccacaa cgacgctatt tgaaggagtt gtcaagaaga caacaaggac
    10561 tggggatatc gactttgcaa aaatcaagac cattctcttg acgactttag ccctatatct
    10621 atcgtctgct attcttcaat actttcaagg ttgggtttta tctggaatcg cggtcaagat
    10681 tacctattca tttcgcaagg atatcttagc taagataaac agaatgcctt ttagggcttt
    10741 tgacagtaca aatcacggag agatcctttc gcgaattacg aacgatgtag atgtggtgaa
    10801 tcaaacgctg actcagacta tttcccagat gatccattcg atcatggcca ttattggtgt
    10861 tttcgtaatg atgctcacca ttagttggca aatgactatt atagcattgg tgacgattcc
    10921 tctttcgctg atcgtggttc gagtcattgt aaagcgttcc caaaaatact ttagaatcca
    10981 gcaagaatac ctcgggcatg tgaatagtca tatcgaagag atttttagtt ctcatatagt
    11041 tgtcaaagcc tatggaggag aacaaaaggc tgtacagact ttcaataatc taaattccac
    11101 cttatatcgg tcagcctgga aaagtcactt tctatcgagc atcatgatgc cgcttatgaa
    11161 tttcgtggga aatataggtt atgtcgcggt tgcggtgagc ggaggctggc ttgcagcaaa
    11221 aaagatggtg acagttgggg acattcaagc ttttctgcag tatgtgcggc agtttacaca
    11281 accaatcgtc cagatcgcga atatctcgaa tgttctgcag caaactgcag cggcagcgga
    11341 gcgtatcttt gattttcttg cttcggaaga agagaatgta tcggtagagt ctccacaaaa
    11401 tggagctctt gtcgaaatac caaaaagtat taaaggtaag gtagaattcc gccacgttcg
    11461 ctttggctat ctgccagggc aacctatcat caaagatttc aacgcaatgg tattgccagg
    11521 tcaaaaagta gctattgtgg gtccgacagg ggctggcaag acgacattgg taaaacttct
    11581 catgcgattc tatgatccgg atagtggaga aatacttata gacgatatac caatcggcaa
    11641 tttgccacga tccgttgtgc gttcctggtt tggtatggtg ctgcaggata cctggctatt
    11701 ttcagggacg gttagaaata atatcgctta tggtaagcgc gatgcgaccg atgccgaaat
    11761 catcgaagct gccaaggctg cccatgtcga tcatttcgtg cgagccttgc ccgaaggtta
    11821 tgatttcgag ataaatgagg aaacaaccaa tatatcgcaa gggcaaatgc agttattgac
    11881 tattgcacgt gctatgttag ctcagcctcc tatgcttatt ctagacgagg cgaccagcaa
    11941 tgtagacaca cacaccgagg tgttgattca gagagccatg ggagtgctga tgcagggtag
    12001 aactagcttt gtgattgccc accggctctc tacgatcaga gatgcggatc taattcttgt
    12061 tatggatcac ggagatatag tggaacaagg gacacatcag gagctgctca gtaaaggcgg
    12121 cttctacgcc gagctctaca acagccagtt cgatcagaat ggcgaagacc aacttgctaa
    12181 cgcttcaggc ctttagtatt tatgatttaa ttggatataa gttaaaaagg cgcccttgaa
    12241 aagggcgcct ttcttttatt ccaatgtatt actgtctggg tattctctca atattccatt
    12301 ccacccattc cggcatttgg agatggcatt gcaggtggat tcttttcggg caggtcggtg
    12361 atggcgcatt ctgttgttaa gaggagcgct gctaccgaag ccgcattctg aagcgcatag
    12421 cgtgtgactt tggctgggtc aattattcct gcctttgtca tatccgtcca ttctagcttt
    12481 gccgcatcga atccgatgcc tttcttttcc tgttttgctt tatctgcgat tatggaacca
    12541 tcgataccag cgttttcggc aatctggcgg attggctcct ccagtgctcg cttgacgatc
    12601 ttgaaaccaa cacgctcatc atcggccatc tttgaggtat cagccttgtc gagagcaaga
    12661 gctgcttgga taagggctat accgccgcct ggcacgatgc cttcttcgat agcggcgcgg
    12721 gttgcggaga gagcatcttc gactctgtgc ttcttttcct tcatttcgat ctcagttgca
    12781 gccccaacat tgattacggc aacgccacca gcgagtttcg caaggcgttc ctggagtttt
    12841 tcgcggtcat aatcgctagt cgtctcttcg atctgcattt tgatctgagc aattcgatct
    12901 tgaatatcct tctgtttgcc gccaccattg ataattgtgg tattatcttt gtcgaccttg
    12961 acggtcttgg ctgtgccaag ctggctaatg cttgtattct cgagctttag gccaagctct
    13021 tcggagataa cctcaccgcc ggtgagtatg gcgatatcct cgagcattgc cttgcggcga
    13081 tcaccaaagc caggggcttt gaccgcacat acattgagtg ttccacgtag atggttcaca
    13141 atgagagtag caagggcttc gccttccact tcttcggcaa tgatgaggag tggcttgccg
    13201 gtttgagcga tcttctcgag tattgggagt agatctttca tgttggatat tttcttgtca
    13261 tggatgagga tgagagggtt ctcaaagact gtggtcatcg aatcgcggtt ggttacgaaa
    13321 tatggcgatg tatagccgcg gtcgaattgc atcccttcga catactcgat ggtggtctcc
    13381 attgtcttag attcttcgac tgtaattacc ccatctttgc cgactttttc catggcatca
    13441 gctatctggt ttccaatgtc gatgtcattg ttggcggaga ctgaggcaac gtgggcaatt
    13501 tcctcttttt ccttgatttc ttttgaaatc ttccgtatct cctcgacaga gaaagttacc
    13561 gcctgatcga ttccgcgttt caatgccatt ggatcgattc cggccgccac ggatttgagg
    13621 ccttccttga caatggaata agcgagtact gtagcggttg ttgtaccatc gccagcaatg
    13681 tcatttgtct tggttgcaac ttccttgaga agctgagcac ccatgttctc atacgggtcc
    13741 tcgagctcga tttccttggc tacagatacg ccatccttgg tcactgtagg agcgccaaat
    13801 ttcttgtcca gaagtacatt tctgcctttg gggccaagag tcactttgac tgcttcagag
    13861 attgtctcta cacctacaag aagctttcgg cgcgcatctt cgctgaacaa caattgtttt
    13921 gccatacctt gttctcctga attgacagtg tttttgccgg ccacatacac atacatcaat
    13981 tataagatgc tgtgaagccg gatattttct ttggatcggc tttgataagg cctgtcataa
    14041 gatataaaaa atgatagaaa acggcaagag gataccacta ttctggacct ttggaatatt
    14101 ctcttaacaa aagaactgaa cctctctttg cttatctcca tataagaggt tcgagacact
    14161 ctagcggatc tatggtacat tagcagtcgt gaaagaatct tgcgcaatcc tttatgccga
    14221 tcaagacctt cttgctattg ataagcccgc aggaatactc gcgatacctg atcgctggga
    14281 cccgaatatt cctgtcgctc agcaacttct tcagagtcaa tttggcacac ttttgcctgt
    14341 tcatcgaatc gataaagaca cgacgggtgt cttgctctat gctagaaatg aagtagcaca
    14401 tcgcataatc agcagcgatt tttcgagtcg taagatagaa aagatctact ttgcgatagt
    14461 acatgggcaa ccggaatctc aggcctggga agtaggactt ccgcttcgcg ccgatggcga
    14521 cagacttcat cgaactatta tcgatgcttc aaaagggaaa gaagctaaga ctcgtttcga
    14581 agtaatagag agatttcggg gctttgctct tgtgcttgct attcctgaaa ccggcagaac
    14641 acatcagata cgcgtgcatc ttgcggcttc tgggcttgcc attgttgcgg accctttata
    14701 tggcaatgga gatcctcttt tgctttctat gataaagcct ggctggaaag gagatgcata
    14761 caaggaaaga cctcttattg cccgaacggc tttgcattcc gcaaaagtga gctttattca
    14821 tccaaaaact aaaaaagaac tgttaataga ggcaccattc ccgcgcgatt tcaaggcaac
    14881 tctagcccag ctacgaaaag tccgcccaag ctgggtatga cacttagcaa gaagtcattt
    14941 ccacgttcta tggaatttcc caagagcatc aacgactctc tcaatctgtt ctgttggtgg
    15001 aagaatagtg gttcggaaat gagcggtacc tggcttctgc ccaaatccgc tgccagctac
    15061 gacacagact ccagtctgct caagaagcgc catgcaatag tcatcatcgc ttttcccagg
    15121 tggtaaggtg atcgaaggga aggcatacat ggctccatct atcggttggc agcggtagcc
    15181 aggaatgcta ttgagccctt cttggagcat ctttgctcgc tttgccaatt cggttaagat
    15241 tgcatttcgc tcattggcaa attgctggaa gctcggatct ccaggttttg ggggacttac
    15301 cataaggtag gtaaggatct gccccggtaa attggcgcag agcgaaatcg attgaagctt
    15361 agttatttga tcaataacct cgcaaggtaa attccgcacc tccatgtacc caccacgatg
    15421 cccgcactca ccaaagaacc cttttgatac cgaatggaag ctaaaaaggc tcacttcttg
    15481 ctcccgcata gaggtcatga catgtgcaaa ggaaataaaa cgtgctcctg gcttatagat
    15541 gttttcttga tatacctcat cagcgagaat cgtgatattg tgcctctttg caaaacgtat
    15601 aaccatgcga atattatctt cagttagcac tcctccggta ggattgccag gatttattac
    15661 gacaattgcg cgagcacgga tgcctttagc tagagcttct tgaattgcat cttctagcat
    15721 ccgctcatcc aggctccaac cggtttcttc atcgagatag tagcctatct gctgtccatt
    15781 atagagagtg attgttgcag aatagagagg atactgcggt ataggtataa gaatgccatc
    15841 atttttctct gcaataagaa gagtgagcgc cgcctggacg cctttggatg cgccatcggt
    15901 aagatagata tgctcggggt ctgcagtctg ttccactcca tcaaagctat cgcgcgattt
    15961 tataaagtca gaaatagcat tgcgcacaaa tctaattcct ttagactcgg agtacgctcc
    16021 aaggccatgt ttggattgct cgaggatatg gcgtgctttt tccttgatat cttccataaa
    16081 aaaattatct gaaatattca gaagtccagg ctgctcacaa gcagcaagcg tgtttcgaac
    16141 ataggtgagc ggcttttgct tcagggcttg tggatttcca atattacaat agatgatttc
    16201 cttgccagct tgctctagct gttgagcacg agccacaatt gggcctcgca ccgcatagta
    16261 agtattgatt acagcatcgc tgagatcgga tttttgaaga ggcataagat ctcctccttt
    16321 gatatcgctt ggatattatc atattctacc acccaaaacg ctggggcgtc gagactgatg
    16381 tcaaatctgc atgaattgtg taatataaaa tgatgaataa aagcatgaca ggagtcaaaa
    16441 ggggcgagca agaaacctcg cgcgtatttc tcatcggtgt cgcatcgagt gaaataccta
    16501 gatcagaggc acaggagctg ctcgacgaac ttcatcggct taccgacaca cttggtaatg
    16561 aggtggcagg tgagatgttg gtactcctcc gcgaatccac accttctctg ttaataggaa
    16621 ccggaaaggc gcaagagata gtagatgctg ccgaccaagc aggtgcggat accattatct
    16681 ttgatcatgt attgagcccg gtgcagcagc gaaactggga aaagctatct tctaagaatg
    16741 tctacgatcg agcggaactt ataatcaaaa tattcgcatc gagggcattt accaaggaag
    16801 ccagcctaca ggtcgagctt gctcagctcg aatatgcatt gccgcgatta gcccattcct
    16861 acaatgacct gatgcagcag cgcggagggc gctatggcac aaaaggttct ggcgagcaga
    16921 aattggagct cgacagaaga gagatagaac gaagaattca tgaaataaaa ggcgaattgg
    16981 agaccgttcg caaagaaaga gccgtccagc gcaagcgcag ggagaggagc gctgtgccaa
    17041 gggctgccat tgtaggttac accaatacgg gaaaatcgag cctcctaaat gagcttactc
    17101 ttgcaagcgt gcatgcagaa gacaaactat ttgctacgct cgatccaacc acaagaagaa
    17161 tacagcttgt tagaggacag accttgctcc tgacggacac tgtcggcttt atccgcaatt
    17221 tgccgcccgg ccttgtggaa gcattccaca gcaccctgga agaagtcagc cttgcagatg
    17281 ttctcattca cgtagccgat gcctcggaca aaagcatcga taaacatatc gaaaccacga
    17341 taaaggtact cttcgatatt ggagccggcg aaataccgca tatactcgtg ttgaacaaga
    17401 tcgatcttgt agatccggta gtggtacagg cttttctttc gcgctaccct ggaagcattg
    17461 ccgtttcggc aaaaacacaa gagggacttg gagagttggt aaaggaaatc caggaagcct
    17521 tgacaaagca gatggagaca ttgatgcttc gcattccaca cgccgacaac catgttgtca
    17581 gcctcatctt acgcgaagcc actgtactcg atcagtatta tgatgaagag tatacctgga
    17641 ttagatgtcg cattccgcgt aaacttatta gagcagtcca gaaatatgca tataaagggg
    17701 ccttgccttt gatggattca attaagccat aattacagac tctttagaaa ggatatttgc
    17761 aaaatgaaga acatcgtttc tgtcgtggtt gttggagctg gcgcgattgg cgcttctata
    17821 gccgtgcgcc ttatggaggc ggggaaacag gtggtaattt ccgctaatgg agagagaaaa
    17881 gctcgttacc taaagcaggg atttgtcgta aatgggcggc agtatttttt gccagtaaaa
    17941 gacaagtatg aggcagatcc agcagacctt gttattattg cagtcaagaa ttacagtctc
    18001 gaagaggcca tagaggaaat gaggccgtat gtcagcgtaa ataccattat tctatcgctc
    18061 ctaaatggta tagatgcagt gcccagactc cgcagagagt tcggtgagga cagtgtgcca
    18121 tatgctatga ttcttggcat cgatgctctt cgtgaagaga atagggttca gtatctggcc
    18181 aagggtagga tcttttgtgg ttttgaaaaa gagaaagtgg aaaagaatgc ttctacgcta
    18241 gccgtcctgg aagagttttt ccgtacgagc gatatcgcat tcgtggtgcc agaggatatt
    18301 gtaaaagaga tatggttcaa attcatgata aatgttggcc ttaaccaatg gtctgctttg
    18361 ataagagctc catatcgtct tttccaagac tctccccatg gccaggagct tcttagtaag
    18421 acaatgatag aagttatttc actttcgaag cgctacggcg gtaatctcga agagacagat
    18481 atcgatcgtg ccattgccgt cttaaagaca cttgcccctc aaggcaagac atcgatgctt
    18541 caagatgtgg aagcaagaag gcagaccgag gtcgatgctt ttgctggtgc aatgaagcgc
    18601 cttgccaagg ccgctgatct cgatgtaccc ataaatgcaa tcttatacga tgccattcgg
    18661 gctatagagg attcattcga ggattcatac aaagcggagt agagatattt ctccagagac
    18721 gaatttcctt ttcttgccat tattgctcag aatgaggctt ccatcttcac tggtacctac
    18781 acatatgcct tccacttcct gcgcaagccg tgggtgtcca ataataaagc gcataagagc
    18841 gcctcgcccc cagagtctgt gctcgtattc agaatgccaa gtggggtcat gcagggcttg
    18901 aatgacatgc tcggttatcg agcaagcggc atcgtcgaga ttggcaaagg ccctcgggat
    18961 aaatcccaga gcttcttcta ggctcagggg cgagagatcg gtgttgagtc cactatagcg
    19021 cgattttaga agattgagcc caatacctat caagacagtg tctgcagtct gctcgcagag
    19081 tatcccacag agctttccaa agcaattaga atcccctgga atgtgggtaa ctatatcatt
    19141 tggccacttc aagaggaaat atggcgctag tttgtgttca tgttgaccat aattgtccat
    19201 gagctctgga aatggttgag tctctaaagc tggatctttc aagctggttt ccaatgctcg
    19261 cactacacca agccctgctc gcagcggtat tgcgctaata tccttataac tttgcggaag
    19321 aattatagtc ataagaagac aatgctctgg catgtcgatc catgctcggc cttcgattcg
    19381 accgcggcct gcagtctgag tatcagcccg gactatcgta atgtgcttta ccagaccttc
    19441 ttgaagaatc ctgcgtgctt catccatagt cgagtcaatg cttttataag ataaaattct
    19501 catagaagat ggctcgatcg atgaggatga gcgcgaatta ttgtccatat gttcaaggct
    19561 atcgaaatcg acatctaagg acaagagcca tggtgagatt gaaaatatac tgtttcagat
    19621 ccttgatttc tctattgcaa aaaatcaaag gttcgataca ctgattttca tgtgtggaat
    19681 aatcggctat tcaggcccaa gaaaagcatc aaaagtcctc cttgaaggac tcagaagact
    19741 tgaatatcga gggtatgatt cggctggaat cgcagtcgga agaactgatc cgaaaccgaa
    19801 cctccagatc atcaaagcag taggaaaaat cggggaactc gcaaagaaaa ttccagagga
    19861 tctggatggc caatggggta taggccacac ccgatgggcg acccatggtg gtgtaacaga
    19921 ggccaatgcc catcctcata cggatatatc cggcaaaatt gcggtggtgc acaatggcat
    19981 catcgaaaac cataagaccc ttcgcacagt gcttgaaaaa aaaggttatg tctttaagag
    20041 cgaaactgat accgaggtca ttcctcatct tatcgcttct tattatgaag gagacctcct
    20101 tacagcagtg ctagcagcac ttcaacatct agaagggact tatggaatag cctgtatcca
    20161 tgcggatgaa ccaggtcgca ttgtgggtgc tcgcaatgga agtcctctca tagttggagt
    20221 gggaaaagac gagatgtttc ttgcgagcga ttttaccgct atggttgctt atacgaatcg
    20281 tgtcatttat ctcaacgatg gtgaggtcgt cgatataacc caggagaatt attccatcac
    20341 ggaccgccat tcaaactcca ttgctaaaaa agtggatgag atcacctggg agcttggtgc
    20401 aatggaaaaa agtggcttca tgcattacat ggaaaaggaa atctttgaac agcctgattc
    20461 gatcgcgcgc gccatgagcg gacgcatcga tgaagagaat gcaacagcca agcttggagg
    20521 cctcaacctt acacgtcgcc agcttgccga agtccatagg gtgcgcataa tcgctgcggg
    20581 cacgagctgg catgcgggta ttgctggttc ttatcttttg gagcaagtag cacgaattcc
    20641 tgcgcaggct gagcttgcaa gtgagctacg atatagaaat ccagttgtag aacagggatc
    20701 gctttggttt gtggtgagtc aatctggaga aacagcagat tctctctttg ctatgcgcga
    20761 aattcagcga aaaggggcca ctgtccttgg aatctgcaat gtcgttggat ctacgatccc
    20821 acgtgagtcc gatggtggcg tctatgttca ctctggtcca gaaatagcgg tcgcaagcac
    20881 taaagccttt acgagtcagc ttactgtctt ttttctcttt accctcctaa tggcccgtat
    20941 gcgagacatg tcgcgagaag aaggcaagat atttatccga gctcttacag gtgttccgga
    21001 tatggtgcgc gatgtgttat ctaagcgcga tcgcatacag gcaatagcca agaaatactg
    21061 tcgcaccaaa gatttcctct atcttgggcg gggcattctg taccctatag ctcttgaggg
    21121 cgctcttaag cttaaagaga tttcatatat ccatgccgaa ggctatggtg cgggcgagat
    21181 gaaacatggc ccgatcgcgc ttatcagccc ggaagtgcca agtgtcttcc ttgtttccga
    21241 cgattatctg cacgataaga ctatatcgaa catgcgagag ataaaggcaa gaggggggcc
    21301 agtcattgct atttgttccg acgaagacaa agaagccatg tcttttgcgg acgactttat
    21361 ttctgtcaag aaagcagatc cgcggttcta tccgttcagc atgattattc cacttcaact
    21421 gtttgcatat ttctgtgcgc tggaactcgg ctgcaatgtt gaccaacccc gaaatcttgc
    21481 caagagcgtg acagtagaat gaagcatggt gcatggcgtg tcttgccaat tgtcgtattc
    21541 gccattgctc ttgtaggggc acttgctttc aacgttgctc tcgtcagcag aagccgcaat
    21601 gctctagcat ctaccattct gcgcaatcca tcgaccattc atagtgctcg ttttcacgtc
    21661 attgctattc ttccggatac tgtggaccca ttttttatgc acctgaaaga gggcttatcc
    21721 gaagaagcag aagcacagga tgcggcactg cagatatttt atttttcgcc tactggcgga
    21781 gccgatagtg gtggtatttc cggagaagtg ttgcgctggt tcgagatagc attgcgcagc
    21841 aaaactgatg gtataattct ttttaagact gccggtctcg atgttgatag gcttatggaa
    21901 gcagccgaaa gtgcacaaat accgtttgtc cctcttgcaa tggatgtttc tcaggaatgg
    21961 ataaacatcg gggtaactgg cgattctgct agtcagggga gagaggcaag cgcgcttgct
    22021 ttgggtatgc tgggcgctgc ggctcgtatc ggcataattc tctcttcaga cacatctttg
    22081 ggctatgctt tctatgagga accattctat agaggagtag aagaaactct caaaagcaaa
    22141 caaggagccg caattgtcgc tgcagagcgc gaagaagaga gcattctcgg cggagaagat
    22201 gcttgcgcgc gcatgttagc tgaacaccca gacatcaatg cgatcctctg catagacgcc
    22261 aaggccacaa ttggtgccgc tcaggttgta atagaccgcg gcatggtagg acaaattgtc
    22321 atcataggag ccgatgaaaa ctcagaggtc aataggctaa tcgaaaaagg cgtggtccag
    22381 gcaagtatcg ttagagatgc agtggctatg ggtaaagctg gcgtggctct agcgattggc
    22441 cagcgaattg gtattcgggc tcctgaaaga atctcggtag gataccgagt gatccccgca
    22501 ctaggagggg gtttatgatc gactttacat ggctctctcg acctctgaga gccaggcgca
    22561 gcagaactgg tcttcgggat cgaataatcg ctaattccgc agtaatacta ctcgttttag
    22621 tcatagcaac atcctacaca gcatctactt cttttaattt ggctcgaagt gtagagcttc
    22681 tatttcacaa ttctgtatcg atgcaagaac tcagaacggt tttgagggca acacaagaaa
    22741 atctgatggg ttatctcagt gctaagaatt cagaatcgct tagagaattt atacgcaatt
    22801 cgacactcct ttccaatatg gctcataagc tcaataaaga aaataagaat gacgatgtct
    22861 ttttgctgga gaaggatctg gcttatcttg tagagagcta tcttgcgagc gctgaaaggg
    22921 ctgtccaggc taagcgcggt cgcgatgtca cagagtatgt tagctactac gaggaagcaa
    22981 ggcgtggagc ttcttacata gagtttttga gtacaagaat ggatacgatc tatttgggcc
    23041 aatcattgac tggtttttct tcatatcgag caaatataag ccttgcaatt atgtccaatg
    23101 gggtgctttt gcttgcggct ttcttgctct cgatcgcact aattgcaaga tattcttata
    23161 caataaccga accattggcg cggctttcag tggctgcagg tgcagtaggc aggggagagt
    23221 ataatcatcc tctgccgcca tatgagcgaa aggatgagat aagaacgctt catgatgcat
    23281 ttgtcaacat gcaggatagt gtccaaaagg cttttataga attgacaaga aaggcggagc
    23341 ttgagcgttc gcttatggaa gagcgcatgc gagtgctaac ttatcagcac aggctaaagg
    23401 atgcggagct cctggctctt caaacacaga tcaacccaca ctttctatat aatactctgg
    23461 cagccggttg gcagatcgct ctatctgaag gtaatgagaa aaccgctgaa ttcctcgaaa
    23521 agcttgcagc attcatacgt tattcattga agccttctac tagattcgtt cttgtttcgg
    23581 aggagataga atgcgttcga caatatatat ggcttttgag gctgagattc ggtgagcgct
    23641 ttgaattctg ccttgacata caggatgaag cccttggctg tgagacgccc gcactcgtgc
    23701 ttcaacctct tgtcgagaat gcaattgtac atggattaaa ggatatcgaa gaaggaggac
    23761 gagtattgat atcagctaag ctggaaggag atacaatcca tttctcggtt agtgatacag
    23821 gctgtggaat gagtaatgaa tgcatcgccc ttgctcttgc agcagcaagc ccagaagata
    23881 caaggcctca aaatggtatc ggattacaca atgtcgtaag gcgagtcgct ctcgcgacag
    23941 gaggtcaggg tagggtcgag atcgagagcg agcttggcaa tggtacttgt gttacgatcc
    24001 tcttgcctgc aggaaaaata tcatgaagag agtgctcata gccgacgacg aaattccgat
    24061 cgtaaatggt cttcagctgc ttttcaaacg ctatttccag gatggctatc tggtggtcgg
    24121 gactgctcag tctggccgcg aagtaatcga aaaaagcaaa actcttgcac cagacatagt
    24181 gcttatggat gttcacatgc ctggaatcaa tgggctggac gcaatacgag aactctccca
    24241 gcaaggtgga gccagggcat ttattctcgt gactgcatat gagcgattcg atattgcgcg
    24301 ggaagccctt tctatgggag tctgcgatta tcttctaaaa cccgtttcca aggaaaggct
    24361 cgagattgcc ttgcaggcgg catcttctta tctcgatcgt cttcatatac tagaggaaaa
    24421 agagcttgaa ttcaaagagc gccagcaaac actattgccc tttattaaaa ctgctttttt
    24481 tgtatatttg cggcagcagc gcgaggctaa agacaaagaa ccaagggacc ccaaacgcac
    24541 aaaggccttg aaagaagagc tcaatcttta caaggaagtg ctacaaattt caggagaaac
    24601 cggtgttatg ggtattgcaa gtttttctcc ggcagatggc ttggcaaatt cgctctatga
    24661 gcatttttgc tctaccttgc aatacaaaac cctggctcta gccggaccgc tcgaagataa
    24721 ccgctattgt gcatggttct tgccattgaa atctgggatt gatcaaggta ctgtcgctga
    24781 gatcgagatc acggcttttc ttgaattgct aggttcatcc ttttcatcac agctcgcaac
    24841 gggcgaattg cgatttgctt gtggtcagcc agaattgctt gagaacattg ggcgctcttg
    24901 ggataatgcc gtacgtaact ttgggcttgg cttagaatcg ataaatcgct acaggcctcg
    24961 cctcggagaa gatattgccg aaaatcgaga agaaccagct tctgtattcg atacacagtt
    25021 ctatgatgcg atcatggaag gtcaatatgc catggctggg cagcttcttg aaaagctcat
    25081 cttgaccgtg gaagagacgc aagacaaacc gcgcgatcta tatttccgaa taatagcagc
    25141 actttcattc tggtcttcta agcttgcggg cggaggagtg ctctccgata atgtctatag
    25201 agatttcatg gattttgggg atatcgaaaa actgtgggag gagcgctttt ggcatttatt
    25261 tgcatcagcg gtaaggggca gatttcagag tctgcagcaa cacgccgcgg aggtgggcac
    25321 tcattcgcct tttgttgtca aggctttgca gtatatagag aaccattacc aggaaccgat
    25381 aacattagag agtgcatcag cggcaatagg catttccgcc ggacatctta cgcgtcttat
    25441 gtcagacgag ctgaagagag gcttcgcgag gactctgata gggtacagaa tgcagaaagc
    25501 caaagaattg cttaaaatgc caaatatctc aatacgagaa gtgagccagc gctgcggtta
    25561 ttccgatgct aattattttg ctcgtctgtt tcgccgactt accggactta cgccgcgcga
    25621 atatgctgca cgggttacaa agggagaaga tagcgatgcc tgaagcgatg cgtaaggcca
    25681 gtggtcctat ttttttcttg atagccatga tcttagcaac taccgctctt ggtttattat
    25741 catgcagcaa atcggaaaag acatctgaga ccaagtcgcc tttgattgga ttttctttgg
    25801 attctcttgt ggtcgagcga tggagacgag atgtggatag cttcaccaaa gcagcacatg
    25861 atctaggggc agaggtggtg cttcgagtgg cgaatcagaa cgcagagacc caaatagcgc
    25921 aagtccgaga gctgttgagt caaggcatag atgtgcttgt aattattcca aatgatgcgg
    25981 aaaggctttc ggaggtatgc cgcgaggcaa aaagaaaagg tgtgcaggta ttgagctacg
    26041 atcgattagt ccatagagct aatgttgatc tctacatttc tttcgataat gaaaaagtag
    26101 gaagcctcca ggctcaagct gttgtggaag ttgttccgca ggggaattat gtcatcataa
    26161 atggagcaac tacagataac aatgctttca tgataaacaa gggttttcat tcggtgctcg
    26221 atccattcat tgaatctggc aagatccaac ttatggaaga gatttggcca agcgattgga
    26281 tgagcgatga ggtgaggaca caatttgaag ctcttgtaaa accgggtcaa aagatcgatg
    26341 cagttctatg tggtaatgat atgcttgcag agacagtgat atccgtgctc tcggagaata
    26401 gaatggttgg aaagaccaag gtgacaggac aagatgccga gctttcagcc tgtcaacgca
    26461 ttgccgaagg aagtcagttt gcaaccgtct ataagcctat cgacagcttg gcgctcaagg
    26521 cggctggctt tgcggtaatg atggccaaag gcgaaaaaat aggatatgag aacaaaatag
    26581 aagatggtac ctataaggtt ccttacattg cgctcgagcc agttctcgta accgcaaaga
    26641 cattggaatc aacagtgata gaggatggct ttcatagtag ggatgatgta tatcgcaatg
    26701 taaaacactg aataaatata tagactatcg agattctaat tctggacatt tttgaagctg
    26761 tcttgtccta aaagtcctaa tactgcgttg ctctggcaat atatgcgcaa ataccggttt
    26821 ttatcacata attcctaatg cgcgattaaa aaatatagcg gaaaatgata gttggcgcgt
    26881 accgaattta attgaagcgc cagactagtc gtattgcgac tagagtctac ggttttcaag
    26941 gaggagtcct catgaaacga acattattga ttgcaatgtt gtttgcagta gttgcgggct
    27001 ttgtatttgc cgaaacacaa gtaggtatcg tgcttcccac caaggatgag ccccgctgga
    27061 ttcaagatca gactcaattt ttggatgctc taaagactgc tggattcagt gcaaaggttc
    27121 tcttcagcca aggcgattcc gcaaaggaaa aagctaacgt cgaagccctg ataagcgaag
    27181 gcataaaagt tctcatcatt tgcccgcatg atggaaccgc tgctgcgtct gcagccgatc
    27241 tcgcccatag cgcaggcgtc aaggttattt cctatgaccg attaattcgc gatacaacca
    27301 gcgtcgacta ctacgtaacc tttgacagct ttgaggttgg aaaagcatgg ggcgattatc
    27361 tcaactccaa ggttccggct ggcacaaaaa gaaacaatct atatctgtat gcaggagcag
    27421 caagtgacaa taactctttc atattctttg aaggagcatg gagcgccctc cagccaaaaa
    27481 tagccgatgg cacatatatc gtgaggaatt cggacaaggc tgcggctctt gcaaagaaag
    27541 ccaagctcac tcgcgatgaa gcagcccaga tcattggtca agtcacgacc aactggaact
    27601 tcagcgatgc caagaacaag gccgaagcaa atctgacagc ggctcctaaa gaagccaaag
    27661 ggaccgtgta tatctgcgct cctaacgatg gcaccgcccg tgcaatagcg gatgcctttg
    27721 ccgcagataa ggatgtcaag acctattaca tcactggcca ggatgccgag atagcttcca
    27781 ttcagtacat catcgacggc aaacaatcca tgactgtcct caaggatgtg cgcacgctag
    27841 taaaggatgc gatcagtgca gctaccgcat acatgaaggg acagaccccg ccggttactg
    27901 cctattacaa caatggcaaa aaagatgtac cggctaagcc gactgccatt gttacggtga
    27961 ccaaagagaa cgtaaagaaa gagatcatcg attctggcta ttggcctgca gacaagttca
    28021 ctggtctcaa gtgatcaatg gggtatgtga gcatgcccat ttgagtacga tcaggagagc
    28081 cgctcattac gagcggctct cgtttgtagt aagatcaatc caggcggtac agattatgaa
    28141 tgacaatatt ctcgaaatgc ggcatataac aaagaccttt ccaggtgtta gagcattgaa
    28201 tgatgtcagc tttgcagttc gccgcggaga gatccactgt ttggtagggg aaaatggggc
    28261 tggaaaatcc acacttatga aggttctgag cggtttatat ccgcacaatg aattcgaagg
    28321 cgagatagtc ctcaatggaa agaaagtaga gttcaatggt atccacgata gcgaggaggc
    28381 aggcgtaggc attatctatc aagagctagc tctggtgccc gagatgaccg tctatgagaa
    28441 cctcatgcta gggcatgaaa tcagacgtgg tatgaccatc gatgtgcacg aaatgataag
    28501 gaaagcagaa gatcttctca agagggttag actttccatc aacccgaccg ctaagattaa
    28561 ggacctaggt atcggcaaaa aacagcttgt cgtgattgca aaagcgctaa gccgaaatgt
    28621 atcgcttctt atcctcgatg aaccgacagc cacattaaac gaagatgact gtgaaaattt
    28681 gctagacata ataagaatgc tgaagatgca aggcgtcact tccatcatga tttctcataa
    28741 gttaaaggag gtgctctcca ttgctgaaac gataacagtt ctccgcgatg gtcagaccat
    28801 atgcaccctg gatcgccgca tggatgacgt caacgagcaa gttctcatta ggcacatggt
    28861 tgggcgttcc atagagaaca tctatcctcc tcgcatatcc aaacctgaag agacaatttc
    28921 cattgaagta aagaattgga atgcttacga tccgaagctt ggacgaaaag tgctgaacaa
    28981 tgtcaatttc tatgttcgca aaggggaggt tgtcgggcta gctggccttg taggctccgg
    29041 aagaaccgaa ttggcgagaa gtctcttcgg caatcctgat aaatatgaga tttcaggtga
    29101 cctcattcta aagggagaga agcggcgctt ctctcatccc agccaagcca ttaaggcggg
    29161 attggcctat gctacggaag accggaaagc caacggcctt atacttatca aatcgatcca
    29221 ggaaaatatt tctcttgcta atttacaaga ggtgtcgaaa aatggcctga tcaacgaaga
    29281 actcgataag cagaccgcct tgagctttgt ggaatccctc tcgataaaaa cgccgggaat
    29341 tcaccagctt gttatgaatc tctctggcgg aaatcagcag aaagtatccg ttgcaaaatg
    29401 gctatttgtt ggacccgatg tgctcatatt cgatgagcca accaggggta tcgatgttgg
    29461 tgcaaaatac gaaatatata acattatcaa cgatctggtg gcaaagggta tgagtatttt
    29521 gatgatatcg tccgaattgc ccgaaatact gggcatgagc gatcgtatct atgtcgtagc
    29581 gaatgggacg attactggcg agctcgatgc aaaatcggca acgcaagaag ctatcatgcg
    29641 ccttgcgaca cagtactgag gaggaagcag atgagtgagt ttatgactgt ggtaaaaaag
    29701 aatgttcgcg aatacggcat gtatatcgcc cttgcagcaa ttatggcggt attcaccatt
    29761 ttatctaagg gtatattcat atcatcgcga aatatcgcta acctcctcaa tcaaacagga
    29821 tatatagccg tgttggcagt cggaatgact ttggttattg taattcggca tatagacctt
    29881 tctgttggct ttctatcagg gtttttaggg gcaattgctg ccatagcttt gactcaatgg
    29941 aattggtcgc tattgccaac aattctattc gtgcttgcgt tgggtgtggt agcaggactt
    30001 tttacagcac tccccgtggc gagtcttggt atccccgcct tcgttgcatc tcttgcaggg
    30061 tggcttatct accgaggcgc attgatgttg gtcaccgcag gtacaggaac tatcatcata
    30121 ccaaacgagg ccttcaatgc tatcggcaac ggctatatcc cggacattct ggctagtgag
    30181 acttttctgc ctggggttca caagacaacc cttctcctgg gggccatcgc catcgtgttt
    30241 gcgattttta gccagatcgc cgatcgaaag cgcaagattc agtacaaatt cgaggtctcc
    30301 tcttccggct tattcgcagc ccagctcatc tttgtttcgg tgattcttgg attcataaca
    30361 tggattttgg caggatataa cggcttatcc tggactattg tgataatgtt ggcggttgtg
    30421 ctggtatacg atttcatcac gcggcgtacg gtgcttggtc gtcatattta tgccgttggc
    30481 ggaaatccgg aggcggccga gttatcgggt atccgcgtca aaaaaataac attcgtcgta
    30541 tttgcatcga tgggcttctt ggctgctctt tccggcattc tattcaccgc caggctcaaa
    30601 tcagcgactc cgcaggcagg cactctcttc gaaatggatg caattgcttc tgcatatatc
    30661 ggtggcgtat ctgcagccgg aggagttggt tctataagtg gctctctaat cggtgctctt
    30721 gtctatatgt cactcatgaa cggcatgaat ctgctgggaa cggatatttc tctacagtat
    30781 ataatacgtg gcttggttct attattggcc gttatcttcg atgttatgac gcgaaaacag
    30841 aaagcctaga tccagaaccc tgattatatt ttgcgttttc atact
//
LOCUS       DAOMZL010000013        29134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_1268_length_29134_cov_14.523299, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000013 DAOMZL010000000
VERSION     DAOMZL010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 29134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29134
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1268_length_29134_cov_14.523299"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1611
                     /locus_tag="PLQ30_02140"
     CDS             169..1611
                     /locus_tag="PLQ30_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HPW01273.1"
                     /translation="MNKKDFPHIHFYDQDFVDIYDRTWAWIADCWTNGGSQTKISKIR
                     FFYYPARKTLDFLEQILSSFFLVYSNRIYPASNGLDALYTLQEPDGAIRSAYDIETGA
                     PIFPEDNPLGVTLPLLSWAEFNLYHKTANKKRVKEIMPILSNYYRWLEANFKMPNGLF
                     STPLSSTGMINSPRSEARYFIDFNATMAMNALYLSALGDILNDKDASFQYKRDYFSIK
                     TRINSKMWNPEDGFYYDLDENEIQVRTKTLGTYWLLLAEIPNEERAEKLIAKLKDPSC
                     FGTDNPFPSLSVDHPAFDKKGQGFRGSVFPWLTFMVIKGLEKYYFYELARNSAIRHLY
                     YILDSMHNSDQEANKRSNHPTVWSAYQPMNEGKAIWEGHPDWPLPNHFPSNGLSTVTL
                     IIENIIGLYISLPRKTVDWIIPNLEVMGIENLSLKRNMITILSSKSGRGWEIHMESEK
                     LYYFTINVLGKKKKTLPIPSGKCSMLIDKI"
     gene            complement(1765..2496)
                     /locus_tag="PLQ30_02145"
     CDS             complement(1765..2496)
                     /locus_tag="PLQ30_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPW01274.1"
                     /translation="MTIVISLGGSIVAPQEGPSGIFLYDFRNVLLSWINGGDHKAIIV
                     VGGGYAARQWQKAYKEMLEMDKAISRDGGSSISRNIDVRQSLDRIGIAATRLNAQLIR
                     EVFSDYCRDPIVTDPSADITMNGKILIAAGWKPGFSTDYDAVILAERFNAEKILNLSN
                     VPQIYSADPKVDPNAKPLFHISFDSLLKMIGTEWDPGANVPFDPIAADKARELGLTVI
                     FASGTNLMNFADILNDRAFIGTIID"
     gene            complement(2573..4225)
                     /locus_tag="PLQ30_02150"
     CDS             complement(2573..4225)
                     /locus_tag="PLQ30_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPW01275.1"
                     /translation="MIHRNSNIDTESLAIRLARFSSITRTSGELSYPDFLISVLREIS
                     FFSNRPNLVQEFEISEDKWKRRIVIAFAPGEGQACITLTGHYDVVDTSNYGALEPLAF
                     DPEALLKKMLQVLSAKEGLSSSEISLKKDLESGYFIPGRGILDMKSGLAAAITVISRF
                     LEDPDRIGNIIFLAVPDEEGASVGMKAAKKILSDFIISHQLQMRAIINLDAAVDQGDG
                     GEAKAVFLGSVSKLLPFVLFVGKAAHAGAPFDGFNPALAASLFIREIECNSDCFSPRA
                     ISPGEEPPPPTILYCRDMRTHYDVTMPFDIFCAVNILTFEKKPEKIFESFRLLVEEML
                     NNAISLFHERKSVFSRKKNEHVAMQRFSSRVIDFSELVDRAEYVAPGILDRLRKAAEK
                     YHPEDPVLQTARIVHELLSFAQIEGPCAIIGFAPPFYAKAELDIYRDQEFLVLLKDEI
                     AQFNREYQETIKLRPFFPGISDMSFLAPSIESHAIEYIRKQSAVIQKDLDEYMDDLIE
                     APVINIGPWGREYHQWGERVNRHYAFKVLPDLVFRISQRILR"
     gene            6298..7626
                     /gene="prs"
                     /locus_tag="PLQ30_02155"
     CDS             6298..7626
                     /gene="prs"
                     /locus_tag="PLQ30_02155"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HPW01276.1"
                     /translation="MSYSDPTHLGIIACPGAEAFANQVIRKLSGIYKHRFMRKAQAIA
                     GRYHITPDLVIRKINFANDCNTGMLFLPGDKERYRPPKFKINVRHTYFANGEIKTEIL
                     ESIRGRSIYIFQDVENHQLVLFNDGTIQKVLSVNDHIFNLFVAIDAAMQAGAAEISLV
                     LPIYPYSRQHKKKGREGLTAARIGKMLESLGVDRIITLDIHSKEIENAFDRLRMENLH
                     ASFQIIEKLTSIIDIAKEDIVVLAPDTGAVDRNKFYANALQKPLALLYKERDYSKITK
                     SATETNISEMRLLGNVSNKVVFMADDMIGTGGTLIKAMRYLKELGASKVICAVSLPIF
                     SANSIDDFDAAYKEGLFYRVIGTNAIYHNELLKKEWYIQSDVTGLFAQVISLLHHDRS
                     LSSLLDNRDVVARLIKRKLESTSSSRTSPNQPKDENANEDVVPYGAANKP"
     gene            complement(7681..8706)
                     /locus_tag="PLQ30_02160"
     CDS             complement(7681..8706)
                     /locus_tag="PLQ30_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HPW01277.1"
                     /translation="MSITTFGEIMLRLKSPGQERLLQSPFFEATFGGSEANVAVALSR
                     LGITARFISILPDNPIGRGCMRELRSHGVDVSCLKFKKGRLGIYFFENGANQRPSNVI
                     YDRENSAISRIAEEDIDWEASFKDMQWFHVSGITPALSQNCADETIKAACIAKDRGLT
                     VSFDLNYRAKLWNYGVDAYSVMCKIAQYADILIGNEEDYQKSLGIKGPSAIGAQQLDI
                     DAYQEMCETALKQYPNAKIAAVTLRESYSADNNDWSAMIVSEGRRFISRKYHISDIVD
                     RVGAGDSFVAGLIYGLITKKDYQAALEYATALSCLKHSISGDFALLEKGEVEKLLGGD
                     ASGRVQR"
     gene            complement(8721..9599)
                     /gene="eda"
                     /locus_tag="PLQ30_02165"
     CDS             complement(8721..9599)
                     /gene="eda"
                     /locus_tag="PLQ30_02165"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HPW01278.1"
                     /translation="MVKKEKIGELFYKTGLIPVIKIDSEEMTDGLAKALRGGGLSVAE
                     ITFRTKAAPKVIERFASCHEDIIVGAGTVTKKDEVDAALLAGAQFIVSPGFNPSICEY
                     CLGKDVAVFPGVNNPSLIEQAMAMGLSILKFFPAEVSGGVKALNAFKSVYQQISFIPT
                     GGIQENNIHEYLSLKNVLACGGSWIVSADLLEARKFDEIERLVKSARRAMMGFLPIVQ
                     DGIIDEENQIRSMRDGGCIGGCIEVKTPSIKRTLAMLSYLKAYVVPGSEVEKGQGLVE
                     VSISIPGLHTQIHLVE"
     gene            complement(9893..10339)
                     /locus_tag="PLQ30_02170"
     CDS             complement(9893..10339)
                     /locus_tag="PLQ30_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HPW01279.1"
                     /translation="MLSDLSRYRGLSKNLDVAFDWLHKGLWRQLSIGKHSVDGDAVFV
                     LIQEYTAKEHSACRFESHREYIDIQMMISGKEIIEVLPANELNVIEPYKPDIEFYASP
                     TDRNANVLAMSAEQLAIFFPEDAHRPCMKAGTLAEPVKKVVVKVAI"
     gene            complement(10482..11243)
                     /gene="kduD"
                     /locus_tag="PLQ30_02175"
     CDS             complement(10482..11243)
                     /gene="kduD"
                     /locus_tag="PLQ30_02175"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HPW01280.1"
                     /translation="MILDEFRLDGRVAIVTGSSTGLGQGMSIALAEAGADVVGVDYVP
                     SKETASIIEAQGCKYLEIVANLLTIEPIQSILDKTISAFGRVDILINNAGTIRREDAI
                     KFNEKDWDEVMDLNVKTVFFFSQAVARKYIEQGTGGKIINIASMLSFQGGIRVPSYTA
                     SKSAVMGLTRLLANEWAKYHINVNAIAPGYMATNNTAELRADRERSAEILSRIPAGRW
                     GTPEDVKGAAVFLASPASNYVNGYTIAVDGGWLAR"
     gene            complement(11272..12102)
                     /gene="kduI"
                     /locus_tag="PLQ30_02180"
     CDS             complement(11272..12102)
                     /gene="kduI"
                     /locus_tag="PLQ30_02180"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HPW01281.1"
                     /translation="MEIREAINSEWAKKMGTEDLRNAFLVEKVFIVGEMTAIYSHVDR
                     MIIGGIVPTSVPLALPVSKELGTEFFLERREIGIINIGGEGFVEVDGTIHDIGQRDAI
                     YVSMGSKNVAFSSKNPKEPAKFYFNSAPAHRACKTRLITLDQANHVALGSDEECNKRV
                     INQYIHPTVLETCQLVMGMTTFSEGSVWNTMPVHTHERRMEVYLYFDMPDDKVVFHLM
                     GKPQETRHIVVRNEQAVISPSWSIHAGVGTGKYTFIWGMAGENQTFDDMDNVEMADLR
                     "
     gene            12292..13062
                     /locus_tag="PLQ30_02185"
     CDS             12292..13062
                     /locus_tag="PLQ30_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPW01282.1"
                     /translation="MADMKVQSLDRAFDILEILAREPTGLTLVEIAAESDLPRSTTFR
                     LLSVLLQRDYVRKGPYDNFYRLGPGFIELSSNYLNSLELKTESAPFMRELASSLGTIV
                     FLARRQGNMMVYIDKEDQFTSLRKYAIIGEQRPIYCTSLGKALILDMDDDEIRNLIPD
                     TFLTKFGPNTHIDVESLIKDIHICKARGWTHDNEEAEPGVNCVAAPIRDYRGQIIASI
                     STSWLLEIRPELEAEKVALFVVRAANEISRTMGFYENL"
     gene            complement(13161..13382)
                     /locus_tag="PLQ30_02190"
     CDS             complement(13161..13382)
                     /locus_tag="PLQ30_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPW01283.1"
                     /translation="MFEQVKSAIENVRPSLKADGGDIELVSVSDDGVVKVRLTGACGA
                     CPYSMMTLKQGIEAYLKKAVPQVKEVKQA"
     gene            13647..14402
                     /locus_tag="PLQ30_02195"
     CDS             13647..14402
                     /locus_tag="PLQ30_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPW01284.1"
                     /translation="MARIIVFVNQKGGVGKTTSAINIGTSLALQYKKTLLIDFDPQGN
                     LTSGIGGKLSHTNIYHVISGLAEIQQIIQSVPVPRLFLAPSSVDLSGATIELVDRPDR
                     NDYLRRAIAPVVNDYEYILIDCPPSLGVLTLNGLAAATEVIIPLQCEYFALEGLSLII
                     QTIGLVQKSINPSLRINGILLTMFDARTRLSQEVVRQVTEYFKDKVFKTIIPRNVRLS
                     EAPSHGLPAIMYDPTCIGAKSYEAVTKEIINRG"
     gene            14395..15306
                     /locus_tag="PLQ30_02200"
     CDS             14395..15306
                     /locus_tag="PLQ30_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPW01285.1"
                     /translation="MAKFGLGKGLGALIPEHQQFFEQSLEETSASIVKLVSIEDLIPN
                     PDQPRKSFTQESLDELAESIRRHGLLQPLLVHQEKSGKYIIIAGERRYRAAQIAGLEQ
                     LPVIVRSQDDENHRIELSLVENIQREDLDPIEEAQAYAKLMEITGVTQERVAEMVGKS
                     RVAVANTLRLLKLPEDMQLLLKQGIISAGHARALLSIEEEKERRKLFEHIQQNNLSVR
                     QAEQEAQKIQNALRGQRTKSKKQLESLKTIEEIPLDPLLKELMEKLIERLGTKVEISG
                     SIDSGTIKIHYFSQEDLERIYEVLKIE"
     gene            15284..16384
                     /locus_tag="PLQ30_02205"
     CDS             15284..16384
                     /locus_tag="PLQ30_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01286.1"
                     /translation="MKFLRLNRAFLDNQYFPHQLKTNRDNGVSEGRRLKAEILTEGRR
                     LRASQAVIRFFVLITIFLLILLANLPAQQPPSSSSFQGIKDISRINSPLPPSFNAVLP
                     KCISFQPGSPILRTSEHIGGELLLAPSLDGVSFIIEHIKAAAKESNLIIEALIFLPNS
                     SVEGPSSISAQLDSLGLIFNQFSSIQGIQYWSASRKIMRTLYVESYRIDNLKSRNRIS
                     DPSTASELKALLSKPMYLHQKDQTFDALILEAHCSITNSYFLMTNKNVTPVRMIGIPV
                     LPINGLRTGFFAAPSPNGTNGILLYFVTSVQSPSIARDRVFESASNKALALLHWFIEK
                     AFSQKLIDPVTLPWNFDDMPADVRVSAIPGKI"
     gene            16528..17829
                     /gene="eno"
                     /locus_tag="PLQ30_02210"
     CDS             16528..17829
                     /gene="eno"
                     /locus_tag="PLQ30_02210"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPW01287.1"
                     /translation="MSIIEYIEAREILDSRGNPTVEVDVVLEDGTLGRAAVPSGASTG
                     EYEAVELRDGDKKRYQGKGVLAAIENVNTIIAGEICGLDSLEQVDIDRTLIELDGTEN
                     KGRLGANAILGVSMATARAAAEYLGLPLYKYLGGVHTNLLPVPMSNIINGGKHADNEI
                     DFQEFMIMPLGAESFREAVRMNAEVFHALKSILKADGHNTAVGDEGGFAPNIGNEDAL
                     DYIIRAIEKAGYKPGEQIAIALDPAASELFEEGGGKGYRFWKSSPDRLFSSDEMIELY
                     TNWINKYPIVSIEDGLDQNDWEGYVKFTKALGNKIQIVGDDFFVTNTKRLAKGIEMGA
                     CNSILIKLNQIGTVTETIETINMAKRAGYSAIVSHRSGETEDTFIADLVVGLETGQIK
                     TGSMSRTDRVAKYNQLMRIEDEMEGIAAYWGHKVLNAVYRP"
     gene            18171..18953
                     /locus_tag="PLQ30_02215"
     CDS             18171..18953
                     /locus_tag="PLQ30_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HPW01288.1"
                     /translation="MLTRDLDRIREAYKRRHPEATRRAYNPSHPHQAEEKHTTTQDKH
                     IKSIRYSGFDGTITTFTAASGVACASLSSGIVLMIRLADLLADGLSMSISYYLSSKSE
                     TEYEADRRARDEWEVENYPEGEKFELKEIYMSRGVSQKDAKPAIEITAKGKIALVDTM
                     MIDELEIILSDESRLANAIATFISLALSGLNPFSTYVFVLFVPSLDSIRFLFACILIL
                     ARIIMFILGSLKIILIERTYSRAALKCCWLDVSQLLLPISFV"
     gene            19008..19556
                     /locus_tag="PLQ30_02220"
     CDS             19008..19556
                     /locus_tag="PLQ30_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPW01289.1"
                     /translation="MKTACMLLAEGFEEIEAVTPIDYLRRAGIYVNVVGIAGKRVRGS
                     HGIIIETDSGPEALEKDYDAIVIPGGMPGASNLANSALVRSLIIRHYEKGKLIAAICA
                     SPAIVLHGACNLLQGREFTCYPGTEDQIRGGHFRAERVVKDGNIITSQGPGTAGEFAI
                     SIISVLEGPQKANEVAQHALLK"
     gene            19745..20125
                     /locus_tag="PLQ30_02225"
     CDS             19745..20125
                     /locus_tag="PLQ30_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPW01290.1"
                     /translation="MEKECIQAKTAPAAIGPYSHAIRAGALVFCSGQLGIDPSTGDLV
                     EGIEAQTNQALRNLVSVLEACSCTVANLVKTTIFLKDMNNFPKVNEIYAGYFDGDFPA
                     RSAIQVAALPKNALVEIEAIAYKQ"
     gene            20137..21309
                     /locus_tag="PLQ30_02230"
     CDS             20137..21309
                     /locus_tag="PLQ30_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HPW01291.1"
                     /translation="MYNFDTIVDRSNTYSIKWDRDQLPEGCRDAIPMWIADMDFACSP
                     DIVKAIQARAEHPIYGYTKCSEEYYKSFIDWMKHRNGWSIEKDWILFSPGVVPAINLA
                     VLAYTQPGDKIIIQPPVYNPFSAAVLNNSRQLVENPLVIRDGRYTMDFEDLDRKIDTQ
                     TKLLILCSPHNPVGRVWTIDELSKLIDICSSHNILIISDEIHSDIILGTEVHHCTASL
                     SEKAAAITITLTAPNKTFNLAGLQIANIVIPNRELSKAFGEQATKIGINLTNTFGMVA
                     QEAAYTKGEPWLEELLSYLKENYATLKSFIDRRIPSLKVFPLEGTYLPWLDCRVLGLS
                     DAELHDLFLKKARLWLDDGTMFGTGGSGFMRINIACPRNVLIKALEQLENAIKTNA"
     gene            21348..21983
                     /locus_tag="PLQ30_02235"
     CDS             21348..21983
                     /locus_tag="PLQ30_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPW01292.1"
                     /translation="MLTYVLVGFALAADAFAVSVSAAACATALPLLIGIRAAFMFGFF
                     QFLMPLVGWSLGSAFSALIQNYDHWIAFGLLIIVGSKMLYEAIHMRRESACDDPEKVV
                     AAKSKTIAATNKGTNSASKGIMKLNTLLVLSFATSIDAMAVGLSYNLLGMPIMLPSFI
                     IGTITFIVCIIGIEFGKRLRKLIQEWAEIGGGSILIIIGFRILIDHIIKGT"
     gene            22081..22755
                     /locus_tag="PLQ30_02240"
     CDS             22081..22755
                     /locus_tag="PLQ30_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HPW01293.1"
                     /translation="MKASSIHRSHKSIQSYDEIQPNEVYCLQNPGGVIFLCTRGSVSD
                     SETSVPHTSLPQDSASLGFSPLPHTSVPRDYRDLPQRYDFAPLSWCIPYEYEPVSKLL
                     VVCDTSHKTFHDIEENRVFAIAFPSFEMRALIQRAGSVSGFDVDKFDHFNVSCFCAKT
                     IDVLIPEGVVGWMECSLERIIIEGTSGILLGAVIKGFAMPESWKRRIHYLNDSIWYKP
                     GDLISD"
     gene            22844..23989
                     /locus_tag="PLQ30_02245"
     CDS             22844..23989
                     /locus_tag="PLQ30_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01294.1"
                     /translation="MKKRLSILSIVLLFASLTTAFAKPGINFSVNSIKTNSDIIAGIP
                     IPSGADIGLEIPIGSGLYSVTFRGAGGYESRMILRNNATSVPISAPSSIDSLNRFFWT
                     NAMAELGVRRYLVRKDDGGKSIWLFGLARGRYEDNATSFPTTVFADAQGMQSISAMLG
                     IAHDSTFWQEPNRKVGWYAEFSMEYSPTFANFTTAQMDFGRFNLTTEGFIPLTPETGT
                     LRNPSMMAPYLVLYGVGDYAVGSHIPHQILTTFGGIKGTKGLGDMVRGAQPWGYEAPI
                     KGYASVELRFPDQSILESFALYPIGYVFGDIGAYGGLYNNPLGTTFIDNSGVIASAGA
                     GVSLSVQNYLWLGAYAGWRFPIIDPLASTYYSKPSGFFWGITFAAHY"
     gene            24073..25029
                     /locus_tag="PLQ30_02250"
     CDS             24073..25029
                     /locus_tag="PLQ30_02250"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPW01295.1"
                     /translation="MNMNKLHLSKSKTRYIIVVFLAILSLSIHPLCAQNQCSVPGLPT
                     IRIATWNVKDCSATNQKTKEAFLLHPFIAEAIKEARIDIIAFQEIQIEGRKGADIALL
                     QDALMSIDWAMPHVAWARSPLNDDLAILSRFPITESQTILAPSEQPWPRPVLEACIDL
                     GDMLLYLYTAHFKAYADQESLSARLAQAQALASHIRQYFGQSIKETAIILAGDFNTVS
                     SYDMQSKIGTVDLLQLRDNEDSTDDFLSLNLEWLNFKPTYKSKSYASITDHIIVSPLL
                     SAYADRDSIEIVDPLLNENGLSLSDHKMLVLNLDRRWLVIQK"
     gene            complement(26133..27497)
                     /locus_tag="PLQ30_02255"
     CDS             complement(26133..27497)
                     /locus_tag="PLQ30_02255"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HPW01296.1"
                     /translation="MSESSQKATVSVSERRYKNGDLARYEIVNFESWEHLYRVFGRTD
                     ADIVKEITRLFQANILPGKTTIGPVILFAVPEGTIDPFQTETEIGFIVNQERRIKYWL
                     TKALARGEFRIEKGNYIFDNSKYESFWESLTHAGMTGIAKINRGYPVLLPLYRDLGTL
                     IDHSKNSIVFNSHFFQMETTDCDTPWDIMGEPFGLLILDGKILLPPLFEREAFFIGKT
                     GNSWIGRPSLRDVEVYIGSTGYIDGYNCRFFSRPAIDRTSKSEGTDLIIVGKHIVGLH
                     DGGCTEIPEGGFIIQTYKRCYPDSFDVDYLGFTNVAFAVQVGPALVREGKAAVGFGPS
                     PFWNGLGPTFPPSVYPLDWERGRAARLGLGSKDGRLLLIWIEGSNKLSYRKGVDSCGA
                     SLAEFAQICIESGVKEFVSLDGGGSAQICIDGGRLLRLSDRYPHSGKDAERPLPFGLA
                     FDLV"
     gene            complement(27494..28327)
                     /locus_tag="PLQ30_02260"
     CDS             complement(27494..28327)
                     /locus_tag="PLQ30_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPW01297.1"
                     /translation="MKYVSHIILYILLIFFALLTAFPFFWMIVNSFSSEAGIFRVPPQ
                     LIPDLIGTNQLWSNYAVIFQQFHFGRYFLNSLFISGASAIGQVIVCSLAGYVFALMNF
                     RGKRLIFSALVFTLMVPAQVTIIPEYFLMMKLNWLDSYLPLIIPSFLVGSFGTFMFKE
                     FYETFPSSISDAGIIDGAGAFRMFTRIYLPQSGPQAATLFIIAFMNSWNDLLRPILYI
                     SDDSMQTVTMALTQFQSQYTARWNLLLAGGVISVMPLLLIFVFLQRYIIENSINSGIK
                     G"
     gene            complement(28337..>29134)
                     /locus_tag="PLQ30_02265"
     CDS             complement(28337..>29134)
                     /locus_tag="PLQ30_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPW01298.1"
                     /translation="PIIGSFYLGFTDWDLLRTPKFIGLSNFSEALSDPGLIRIVVNTV
                     LFSIIYVIGVMVIGLFLALMVNQKLPGTNFFRAAYYLPVITSAVAIGIIWSWILSPQV
                     GILNILVKSVGLKPPNWLGDTHWALLTVSVIQVWKMAGYYMVLFLAGLQSIPDSVKEA
                     AVVDGANSLQLFFHVTLPMLSPTTFFVLTVAMIDSFKNFELIYTMTRGGPQSSTTTLV
                     YDVYVYGFVHYRMGYASAVAYVLMVLVGAITIANFYIKKRWVTNSYY"
BASE COUNT         8635 a         6608 c         5708 g         8183 t
ORIGIN      
        1 caatcaatga tgaatcaacg gcatttaata atcgataaat ttgacaaata gatatggaat
       61 gagtatagta agttgcgata tgtgtgaata taatgcaatt ttctgtaaag ttttccagtt
      121 atctataaat tcagcaaatt gcagacatac aataagaggg taagtttctt gaataaaaag
      181 gattttcctc atattcactt ttatgaccaa gactttgtcg atatttatga tcgaacatgg
      241 gcatggattg ctgattgctg gacaaatgga ggtagccaga ccaaaatcag caagatcaga
      301 ttcttctatt atcctgcaag gaagactctt gattttctgg agcagatctt gtccagtttc
      361 tttctagtat atagcaatag gatttatccg gcatcaaatg gactggatgc cctttatacg
      421 cttcaagagc cagatggcgc aattcgcagc gcctatgata tagagacagg cgctcctatt
      481 ttcccggaag ataaccctct tggcgtaaca cttccccttc tctcatgggc agagttcaat
      541 ctctatcata agacagccaa taaaaaaaga gtaaaagaaa taatgccaat actgagcaat
      601 tactacaggt ggcttgaggc aaatttcaaa atgccaaacg ggcttttttc tacacctctt
      661 tcttctacag gcatgataaa ttctccacgt tcagaggctc gatattttat tgatttcaac
      721 gcaaccatgg ctatgaatgc cctatatttg tctgctctgg gggacatact taatgataag
      781 gacgcttctt ttcaatataa aagagactat ttttccataa agacaagaat aaattcgaaa
      841 atgtggaacc cggaagacgg cttctattat gatctggatg aaaacgagat ccaagtacgt
      901 acaaaaacgc tagggacata ttggctgcta ttagccgaaa ttcctaatga agagcgtgcg
      961 gaaaaactta tagccaaatt aaaagatccc tcctgttttg gtactgacaa ccccttccct
     1021 tcgctttcag tcgatcatcc agcctttgat aaaaaggggc agggattccg aggaagcgtt
     1081 tttccttggc ttacatttat ggtcatcaag gggttggaaa aatactattt ttatgaactt
     1141 gccagaaact cggcaattag gcatctctat tatattctag attccatgca caacagcgat
     1201 caagaagcta acaagcgcag caaccatcct actgtttgga gcgcctatca gcctatgaat
     1261 gaaggaaaag ccatttggga aggtcatccg gattggcctc tgccaaatca cttcccctcc
     1321 aatggcctca gcaccgttac cttgataatt gagaatatta ttgggctata tatatcgctg
     1381 ccccgaaaaa cggtcgactg gataatccca aatcttgaag ttatgggtat tgagaacctt
     1441 tctcttaagc gcaacatgat aaccattctt tctagcaaat caggaagagg atgggaaatt
     1501 catatggaga gcgagaagct atattatttc accatcaatg tattaggcaa aaagaagaag
     1561 acgctcccca taccttccgg aaaatgttct atgctcatcg acaagatata gaaaaatcaa
     1621 gtcttgcgcc aacttcagat cttggagtca agaacaagaa caatctgaaa tgttaaaaca
     1681 catgtgagcc atacaggaaa ttgccaagtt cgaatgtccc ttcgatctgg cgccttaagc
     1741 cgatcaattt agaacagaat aaatttaatc gattattgtg cctataaatg ctctatcatt
     1801 taaaatatca gcgaaattca tcaagttggt ccccgatgca aatatcaccg taagaccgag
     1861 ttcgcgagct ttgtcagccg caattggatc gaagggcaca tttgctccag gatcccattc
     1921 ggttccaatc atcttcaaaa gagaatcaaa agatatatga aaaagcggct ttgcattagg
     1981 gtcgaccttt ggatcggcac tataaatctg agggacatta gagagattca atatcttttc
     2041 tgcattaaaa cgctcagcaa gaataactgc gtcatagtcc gtagaaaacc ccggtttcca
     2101 gccagcggca atcaatattt tgccgttcat tgtaatatca gcggaaggat ctgtgactat
     2161 tggatctcta caataatcag aaaaaacctc cctaatcaac tgtgcattga gccttgttgc
     2221 tgcaatgcct attctatcaa gagattggcg aacatcgata tttctgctta tgcttgatcc
     2281 tccgtccctg ctaatcgctt tgtccatttc aagcatttcc ttataagcct tttgccactg
     2341 acgagctgca taacctccac caaccacaat gatcgcctta tgatcccccc catttatcca
     2401 ggaaagtaat acatttcgaa aatcatataa gaatatcccg ctaggtccct cttgtggagc
     2461 aactatggaa cctcctaaag atatcacaat agtcatcatt attccccctt ttgtttatat
     2521 gatgtcttct ctatattcat ataaggtata attaaataat gaattatttg agctatctca
     2581 atatcctctg gctgattctg aatactaaat ccggcaagac cttgaaagca tagtggcgat
     2641 ttaccctttc tccccattga tgatattctc taccccatgg accaatatta attacaggtg
     2701 cttctatcaa atcatccata tactcgtcca aatctttctg aattaccgct gattgctttc
     2761 gtatgtattc tatcgcatga ctctctattg atggtgctag aaaactcata tccgaaattc
     2821 caggaaaaaa gggacggagc tttattgttt cttgatattc tctattgaac tgagcaatct
     2881 cgtctttgag cagaaccaaa aactcttggt ctctatatat atctaattcg gcttttgcat
     2941 aaaaaggagg agcaaagcca ataattgcgc agggtccttc gatttgggca aatgaaagca
     3001 attcatgcac tattctagct gtttgtaaca cgggatcctc gggatgatac ttttcggctg
     3061 cttttcttag tcgatccaga atacccggtg caacatattc agcacgatca accaactccg
     3121 aaaaatcaat aaccctcgaa gaaaatctct gcattgcaac atgctcattc ttttttctgg
     3181 aaaaaacaga cttccgttca tggaagaggg aaatagcatt attcagcatc tcctccacaa
     3241 gtagcctaaa actttcgaat attttttcag gtttcttttc aaaggtcaga atattaacag
     3301 cacaaaatat atcaaaaggc atagttacat catagtgtgt ccgcatgtct cggcagtaga
     3361 gaatggttgg cggaggcggt tcctctccag gagatatagc acgagggcta aagcaatcgc
     3421 tattgcattc tatctctctt ataaaaagag aagcagctaa cgccgggtta aagccatcaa
     3481 aaggtgcacc tgcatgagct gctttcccta caaacaaaac aaatggaaga agcttgctaa
     3541 ctgatccaag aaatacggct ttggcttcac ctccgtcccc ttgatcgaca gcagcgtcca
     3601 aattaattat tgctctcatc tgaagctgat gagaaataat aaaatctgag agaatcttct
     3661 tagctgcttt cataccaaca gaagccccct cttcatctgg cactgctaag aaaatgatat
     3721 tcccaatcct atcaggatct tccaagaacc tagaaatcac agtaattgca gcagctagtc
     3781 cacttttcat atcgaggatg cctctgcccg gaatgaaata gccgctttcc aaatcctttt
     3841 tgagagatat ttctgaagag gaaaggccct ctttagcaga aagcacttgg agcatttttt
     3901 tcaaaagcgc ctctggatca aaagcaagag gctctaaggc tccataatta cttgtatcaa
     3961 ccacatcata gtggcctgta agcgttatac atgcttgccc ttctccggga gcaaaagcaa
     4021 ttacaattct ccgcttccat ttatcctcag aaatctcaaa ttcttgaaca aggtttggcc
     4081 tattactaaa gaaggaaatt tctcgaagaa cagaaataag aaaatcagga taagataatt
     4141 ctcctgaggt tctagttata ctagaaaatc tggccaatcg aatcgctaaa gattcagtat
     4201 ctatgttact gtttctgtga atcatataga tgctacttta tcataaattt gaaagcttgg
     4261 ttcagtattt tcacttgaat tgatgagaca tatgatgaca tccctcgaca taatacaaat
     4321 ttaggaatat caggataatt gagcaataga gggacggagg tttatcttat attttatcct
     4381 atatttggca tgcagcacta gcccgtacct aatttattaa tctgcaatga tttaatagta
     4441 gctagatatt actaaatcgt actcaaatct actgctctct ctgtgctact tacaagttgg
     4501 aaacgggtta ttgagaattt tcaagtgata gatcatcaat tttgaattat tccttcttct
     4561 ttctgcgatg tttttaatat gagacattca aggatattat cagaaggatc ttggtaccat
     4621 gtatatgcca agatcaatag agcagaatgt atatttgaca ataaagggat aaaggacctt
     4681 tttctcaagg tagttctgcg tgcaaaaact aaagcaagac atccttataa atttgaaatt
     4741 gcaaattttt gtataatggg aaaccacatt cattttttaa ttagaccagc aagaggagaa
     4801 tctctatcca aaataatgca atggatatta tccgtatttg ctatacatta taataaaata
     4861 atacaatcta aaggtcatgt atggtatgac cgtttcaaga gcgtgctaat taacaccata
     4921 aggcaattta ttttggtata tgaatatata agcaataacc ctataaaagc gggcctttgc
     4981 acattatcct cagaatatcg atattgcggt gcatacttca tttcaaaaaa tgattttcgg
     5041 attatagaga aagatttcga gttataatca aaataacata gtaagccctc aagactaaaa
     5101 caaatcagca ggataataaa gtaattcaat attttttgta taaatagcga caagcaggca
     5161 tgaagtctat tatgctcaag tagtacaaaa aggaatcaac gcaaaatcgg cggttgaagt
     5221 aaccaaggca aaaagactat tcaggaaatc ggcatgattt tgcaggtaca tccaaaccca
     5281 aatgcatacc aatctagtcg ccttttggaa aaaaaacagc tttcggggaa aagggctccg
     5341 ctcttctaga aagacacaaa gaacaaagaa acatagaaaa ccataatcaa aacagaactg
     5401 tccaagcaaa acggacaatt acaagctgag aacgaattct tcaaagaata ctaacaactg
     5461 gaccgcacca aaccaaaatg gtatagcaag atctgaggct ttgcttgcgt cgccaatttg
     5521 accttttaag gatatcacga atgtcctatt gctggggagc tagtctcata cgaaaaaaca
     5581 atgacgacct ctaaatgctc atggaaatcc ttgtgattcc acgcgataaa tctatcttcc
     5641 gatatctcaa tattgcgtat gaacttacaa atctttatat gacacggaag catgcgcaaa
     5701 ggattatgca aagagcgatt ttgcgagcca tttatacagg ggaacagttt tcaaaaccgg
     5761 cgacaagcat aaagaaatgc cactatctct tgcgatataa ggcaaaaggc ggcctaccga
     5821 atcgtattaa ggtcgcacat acgaccactg cgaaaatcga tcatggttgt gcgcatttag
     5881 cgatggctgt tgacctgtct caagccagat tcctgcgcgg agcgtttcaa aatccaaata
     5941 ttctgtgggg cactcaagta caaaggcatc cgaataaaga aacacatggc atgaggtgca
     6001 taagattcac attaaaaagc agaacaaaag gctttataca aagggacgca gtatagcgct
     6061 tcaagaattg gcacaaatcc acagattctc aaatgccagc atcgacgctt ttcgtgatct
     6121 tggatcctgc atagtgctcc gcatacagag caaatgaatt tgctcccttg ggatgaccca
     6181 ctacaattct catctaagag attctattca tttggaagat tatggtctat aatagcaacg
     6241 atctctcttg ttactcatcc gcgtcagttt attgaattca aatacggaag gccttgcatg
     6301 agttattcag atccaaccca tttaggcatt attgcttgcc caggagcaga agcgttcgca
     6361 aatcaggtca tccgaaagct ctcaggaatc tataagcatc gattcatgag aaaagctcag
     6421 gctatagctg gaagatatca tatcacgccc gacttggtta ttagaaagat caactttgca
     6481 aatgactgta acacaggcat gcttttcttg ccaggagata aagaacgtta tcgacctcca
     6541 aaattcaaga ttaatgtaag acatacttac tttgcaaatg gtgaaataaa aacagaaatc
     6601 cttgaatcta tccggggaag aagcatctat atttttcagg atgtggaaaa tcatcaactc
     6661 gtcttattta atgatggtac tatccaaaaa gtcctttctg taaatgatca catttttaac
     6721 ctttttgtag ctattgatgc tgccatgcaa gctggagctg cagaaataag tcttgtgcta
     6781 ccaatatatc cctattcgcg acaacataag aaaaagggaa gagagggatt aactgcagct
     6841 cgcataggca agatgctgga aagccttggt gttgatagaa tcatcactct tgatattcac
     6901 tcaaaggaaa ttgagaatgc atttgaccgg ctcagaatgg aaaacctaca tgcctctttt
     6961 cagataattg aaaaacttac atcgattatc gatatagcaa aagaagacat agtggtgctc
     7021 gcacccgata ccggtgcggt cgatcgaaat aaattctatg caaatgcttt gcaaaagccc
     7081 ttggctttac tttacaaaga aagagactat tcaaaaatca ctaaaagcgc aacagagaca
     7141 aatatcagcg aaatgcgatt gctcggcaat gtctcgaata aagttgtctt tatggcagat
     7201 gatatgattg gaactggtgg aactcttatt aaggcaatga gatatctgaa agaacttggc
     7261 gctagcaagg ttatatgtgc tgttagtctt ccaatttttt cagcaaattc tattgatgac
     7321 tttgatgctg cttataaaga agggttattc tatagagtaa taggcacaaa tgccatatat
     7381 cataatgagt tgctaaagaa agaatggtat atccagagcg atgtcactgg cctttttgca
     7441 caggtgatta gcttactgca tcacgataga tccttaagct ctctgttgga taatcgtgat
     7501 gtggtagcac gcctcattaa gagaaagttg gagagtacga gcagcagtag aacctctcca
     7561 aatcagccaa aggatgagaa tgcgaatgaa gatgttgttc cttatggcgc agctaataag
     7621 ccatgagact taccatcatc cttttattaa tcaaagccag agcatatttg agaaagcgaa
     7681 ttaacgttgc actctaccgg aagcatctcc tcctaagagt ttctcaacct ctcccttttc
     7741 aagaagggca aaatcgcctg aaatagaatg ctttaggcac gaaagggcag ttgcatattc
     7801 aagggctgct tgataatcct tcttagtgat taatccgtat attaagccgg cgacaaaact
     7861 atctccagcc ccaactctat ccacaatatc cgatatatgg tatttgcgag agataaacct
     7921 ccgtccctca cttacaatca tcgctgacca atcattatta tcggctgagt aactttctcg
     7981 aagagtcact gctgctatct ttgcattcgg atactgtttt aaagcagtct cgcacatctc
     8041 ttgatacgca tcaatatcaa gttgttgagc tccaattgca gaaggacctt taatccctaa
     8101 tgatttttga taatcttctt cattccctat aaggatatcc gcatattggg caattttaca
     8161 catcacacta tatgcatcta ctccataatt ccataatttg gctctataat tcaaatcaaa
     8221 agatacggtt aatcccctgt ctttagcaat gcaagcagct tttatcgttt catcagcgca
     8281 attctgagaa agagcaggtg taattcccga tacatgaaac cattgcatat ctttaaaaga
     8341 agcttcccag tcgatatcct cttcagcaat tctggaaatc gctgagtttt ctctatcata
     8401 aatgacatta cttggacgct gattggctcc attctcaaag aaatatattc ccagccttcc
     8461 ctttttgaat tttaggcatg aaacatcaac accatgagac cgaagctctc tcatgcatcc
     8521 tctgccaatc ggattgtctg gcaaaatgct aataaatcgc gccgtaatgc ccaatcttga
     8581 cagagcaaca gccacatttg cttcgcttcc accaaaagtc gcttcaaaaa aaggcgattg
     8641 aagcaatctt tcctgtccgg gagattttaa ccgaagcata atctccccaa atgtcgttat
     8701 gctcattcta attctcctcc ctattctaca agatgaatct gggtatgcaa tccaggaata
     8761 cttatactca cctccaccaa accttgtcct ttttcgacct cagaaccagg cacaacatag
     8821 gctttcaaat aggaaagcat agctaatgtt cgtttgatcg atggggtttt gacttcaata
     8881 cagcctccaa tacaacctcc gtccctcatt gatctaatct ggttttcttc atctattatg
     8941 ccgtcctgca cgattggcaa gaatcccatc atcgctcgtc gtgcagattt tacaagtctt
     9001 tcaatttcat caaatttacg agcttcaaga agatcggctg aaacaatcca tgaaccacca
     9061 catgccaata cattcttcaa agaaaggtat tcatgaatgt tgttttcctg aattccccct
     9121 gttggaatga atgatatttg ctgatatact gatttgaatg cattaagagc cttaactcca
     9181 cccgaaacct ctgcaggaaa gaatttcaag atactaagac ccatggccat agcttgttct
     9241 ataagcgaag ggttgtttac gccggggaaa acagcaacat cctttcccaa gcagtattca
     9301 catatagatg gattaaaacc tggcgaaaca ataaactgcg ctcctgccaa aagagccgca
     9361 tctacttcat ctttcttcgt cactgttcca gcacctacaa taatatcctc atggcacgat
     9421 gcaaatctct cgattacttt tggtgcagcc tttgttctaa aagtgatctc ggcaacagac
     9481 aatcctcctc cgcgaagtgc tttagctaga ccatctgtca tctcctcaga atcaatcttt
     9541 atcacaggga tgaggccagt tttataaaac agttctccta ttttctcttt ttttaccatt
     9601 gcataatcct caaaaatgtt acgtactagt ccacatagcc aaagtcaagt ctcgtcttta
     9661 gctagagccg acaaaatatc aaatcgacag aatatttcag tcctaattgt tctgtgacgg
     9721 aatcctccgt ccccgatttc ctccccgatt tcatgcattc ccaattttcc agactttttc
     9781 agtacgcaat tcgcaatcga aattccagct agataagatt tgccctctta cctctcttct
     9841 ctttctgatc aaaatcagta aagcgaacct tcttctagcc ttaaggctta tattatatag
     9901 ccactttaac cacaactttt ttcacaggtt ccgccaatgt tccagctttc atgcatggcc
     9961 gatgagcatc ttcaggaaaa aaaattgcaa gttgctcagc actcatcgcc agaacattag
    10021 catttctatc tgttggtgag gcataaaact ctatatcagg cttgtaaggc tcgatgacat
    10081 tgagttcatt tgctggcagc acctcaataa tttcttttcc tgaaatcatc atttgaatgt
    10141 ctatatattc tcgatgagat tcaaatctgc acgctgaatg ctccttagct gtatattctt
    10201 gaatgagcac aaagacagca tcgccatcaa cagaatgctt tcctattgaa agctgacgcc
    10261 aaagcccctt atggagccaa tcaaacgcga catctagatt cttactgagt cctcgatatc
    10321 ttgatagatc tgaaagcatg caaacaatca tctttttact ccttatatag gcatttccga
    10381 attaataaat aatcgctctt atcaataatc aacaaacctc cgtccctcct gccactaaag
    10441 ccataaattc tacacaagga accatatctt ttttatgaca gttatctagc tagccagcca
    10501 ccatcgacag caatagtata cccattcaca tagttgcttg ctggtgatgc taagaagact
    10561 gcggcgcctt tgacatcctc aggcgttccc catcgacccg ctggtatacg acttaggatc
    10621 tccgctgatc gctctctatc cgctctcagt tctgccgtat tgttagttgc catatagcct
    10681 ggagcaattg cgtttacatt gatatgatat ttagcccatt cgttggccaa taaccttgta
    10741 agacccatca cagcactttt cgaagctgta taagacggaa ctcttatacc tccttgaaat
    10801 gaaagcatgg atgcgatgtt gatgatcttt cctcccgtcc cttgttcgat atatttccgc
    10861 gcaactgcct ggctgaagaa aaatactgtc tttacattca ggtccatgac ctcgtcccaa
    10921 tccttctcat taaacttaat tgcatcctct cgcctaatag tcccagcatt attgataaga
    10981 atatccactc gtccaaaagc acttatagtc ttatctaaga tgctctgaat tggttctatt
    11041 gtcagaaggt tagctacgat ctccaagtac ttacaccctt gcgcctctat gatgctagcc
    11101 gtttcttttg atggcacata atctactccc acaacatcgg ctccagcttc tgctagcgcg
    11161 atgctcattc cctgtccgag accagtcgaa ctacctgtga caatcgccac tctgccatcg
    11221 agcctaaatt catccaaaat catgtctttt ctccttttaa aacagatctg tttaacgtag
    11281 atcagccatt tccacattat ccatatcatc aaatgtctgg ttttctccag ccatgcccca
    11341 aataaacgta tatttgccag ttcctactcc tgcatgaatg gaccagctcg gacttattac
    11401 ggcttgctcg tttcgtacca caatatgacg cgtttcctga ggcttgccca tcaaatggaa
    11461 tactacttta tcatctggca tatcaaaata cagatatacc tccatccgcc tctcgtgtgt
    11521 atgcactggc attgtgttcc agacactccc ttctgaaaaa gtcgtcatgc ccattacaag
    11581 ctgacatgtt tcaaggactg ttggatgaat atattgattg atcactcttt tgttgcactc
    11641 ctcatcacta cccaacgcca catggttggc ttgatcaaga gtgataagcc tagttttgca
    11701 tgctctatga gcaggtgcac tattgaaata gaatttcgcc ggctccttag gattcttgct
    11761 tgaaaatgct acattttttg aacccatcga gacatatatt gcatcccgtt gtccaatatc
    11821 atggatagta ccatctacct cgacaaaccc ctctcccccg atatttataa ttcctatctc
    11881 tcgtcgctct aaaaaaaact ctgtccccaa ttccttgctc actggcaacg caagagggac
    11941 ggaggttggg acaatgcctc caattatcat cctatctaca tggctataga tggctgtcat
    12001 ctctccaaca ataaaaacct tctccaccag aaacgcattg cgcaaatcct ctgtccccat
    12061 tttttttgcc cactcagaat taatcgcttc tcgaatttcc atctttgctc cttgcatcat
    12121 aaataatgga acataatttt taggtctaat gcctatgttt agcattgcgc ccaaaacgtc
    12181 ctgtaaattg attccacaat atggaatcca attccatata atagcttgct tttctatctt
    12241 gtcaatacta aaatatatct ttatggttag attaatgaat aaggaggcct tatggctgat
    12301 atgaaagtac agtctctcga tcgcgccttc gatatactcg aaatccttgc acgggagcca
    12361 actggtctta cccttgtaga gatcgctgca gagtcagatc ttcctcgcag tacgactttt
    12421 cgtttgcttt ctgttcttct ccagcgcgat tatgttcgaa aaggcccata cgataacttt
    12481 tatcgcctgg gaccaggttt tatcgagctt tctagcaact atctgaatag tctagaactc
    12541 aagacagaat ctgctccctt catgcgggaa ctagcatcgt cgttaggaac gatcgtattc
    12601 cttgcacgtc gtcaggggaa tatgatggta tatattgata aagaagatca gttcactagc
    12661 ctgagaaaat atgcaatcat aggcgaacaa aggcctatct attgcacatc tctgggtaag
    12721 gctctcatat tagatatgga tgatgatgaa attcgtaatc ttatccccga tactttttta
    12781 acgaaatttg gtccaaacac gcatattgat gtggaatctc tcataaaaga tattcatatt
    12841 tgtaaggcca gagggtggac tcacgacaat gaggaggcag agcctggagt aaattgcgtg
    12901 gcggctccaa ttcgagacta tcgtggtcaa atcatcgctt ctatcagcac atcatggctt
    12961 ttggaaattc gaccagaatt ggaggcggaa aaagtcgcct tatttgtagt cagggcagct
    13021 aatgaaatat caagaacaat gggattttat gaaaatctct gaaaattagc aaaagaccag
    13081 gctttgcgaa aatagtaaat ccaatctaga acaaatagaa attaagccgg agtgcaattc
    13141 tttccagcaa ctagaatttt ctaggcttgt ttgacttctt ttacctgtgg cactgctttc
    13201 ttgaggtacg cttcaatgcc ttgcttgaga gtcatcatgg aataagggca tgcaccgcat
    13261 gctccggtga gtctcacttt gaccacacca tcatcactta cagatacaag ttctatatct
    13321 cctccatctg ctttgagaga tggtcgtaca ttttctatcg cggatttaac ctgttcgaac
    13381 agcattcata tctcctttat taagattaaa ttatcaaatt tgaccaagat aggtcaagaa
    13441 ggctggctag actcttacaa gcttagactt ttcagtcttt tatagacact caagatagca
    13501 acataattga ataccaatag acaaaactat tttggcatta ttttcgatat gtatgtaatt
    13561 gtattaactg ctttttatgc gctaaacctg tatagctgaa gctcgatcaa tatgttagaa
    13621 tcattttagc atatcgggaa ggcagaatgg ctcgtattat cgtatttgtc aaccaaaagg
    13681 gaggtgtagg gaaaaccacg agtgctatta atattggtac atccctcgcc ctgcaatata
    13741 aaaaaaccct cctcatagac ttcgatcccc agggaaacct cacatcagga attggaggta
    13801 agttatccca caccaatata tatcatgtga tttcaggtct tgcagagata caacaaatca
    13861 tccagtcagt ccctgttccg cgccttttct tagctccctc ctcggttgac ctcagtggtg
    13921 caactatcga gctcgtcgat cgtcccgacc ggaatgacta tctaagacgc gcaattgccc
    13981 ctgtggtgaa tgattatgaa tatatcctta tagactgtcc tccgtccctt ggagtgctta
    14041 cgttgaatgg actggcagcc gctacagaag tcataatacc cttgcagtgc gaatatttcg
    14101 cattagaggg actctcgctg attatccaga cgatcgggct tgtacaaaag agcatcaatc
    14161 cttctttaag aatcaatggc atcctgctca ctatgtttga tgctcgcacg cgactttctc
    14221 aagaagttgt caggcaagta accgaatatt tcaaggacaa agtcttcaag acaatcatcc
    14281 cgagaaatgt ccgtctctca gaagctccat cgcatgggct ccctgccatt atgtatgatc
    14341 ccacctgcat tggagccaag agctatgaag ctgtcacaaa ggagatcata aaccgtggct
    14401 aaattcggtc ttggaaaggg acttggcgcc ttaattcccg aacatcagca atttttcgaa
    14461 caatctcttg aagaaacatc cgcatctatt gtgaagcttg tatcgataga agacctcatt
    14521 cccaatccag atcaaccaag aaaatccttc actcaggaat ccttagatga gcttgctgaa
    14581 tctattcggc gtcatggact cttgcagccg cttctcgttc atcaagaaaa atcaggaaaa
    14641 tacatcatta ttgctggaga acgaagatat cgcgctgcgc agattgcagg gctggagcaa
    14701 cttcccgtca tagtacggag tcaggatgac gaaaaccatc gcatagagct gtctctggta
    14761 gaaaacattc agcgtgaaga ccttgatccc atcgaagaag ctcaagctta tgcaaagctg
    14821 atggaaataa caggagttac tcaggaacgc gtcgccgaaa tggtgggcaa aagccgcgtc
    14881 gctgtcgcaa atactcttcg cctgcttaag cttccagaag acatgcaact tctgctcaaa
    14941 caaggtatta tcagcgctgg acacgccaga gctcttcttt ccatagaaga agaaaaagaa
    15001 cgtagaaagc tctttgaaca catccaacag aacaatttat cggttcgcca agcggaacaa
    15061 gaagctcaaa aaatccaaaa tgcgctccga ggccagcgga caaaatctaa aaagcagctt
    15121 gaatctctta agacaattga ggaaattcct ctcgaccctc ttttaaaaga gctcatggaa
    15181 aaactcatcg aaagattggg tacaaaagtc gagatatcag gtagcattga ttccggtacc
    15241 ataaaaattc actacttctc ccaagaggat cttgagcgaa tctatgaagt tcttaagatt
    15301 gaatagagct ttcttggata atcaatattt tccccaccaa cttaaaacca atagagacaa
    15361 tggagtctct gagggacgga ggttgaaagc tgagatacta actgagggac ggaggttgag
    15421 agcttctcaa gctgttatcc gcttttttgt tttgatcacc atttttctgc taatccttct
    15481 tgcgaatctt ccagctcaac aaccgccatc gtcgtcaagc tttcaaggta taaaggacat
    15541 ctctagaata aactcccctc taccaccatc ttttaatgca gttttgccca agtgcattag
    15601 ttttcaacca ggctctccca tccttcgcac gtctgaacat attggaggcg agcttttgtt
    15661 agctccgtcc ctcgatggtg tctcgtttat aattgagcac attaaagctg ctgcaaaaga
    15721 gagcaatctt atcattgaag ctctgatctt cctgcccaac agctctgttg aaggtccatc
    15781 ttcaatctct gctcaacttg attcgcttgg gctaatattc aaccagtttt cttctataca
    15841 aggcattcag tattggtcgg caagcagaaa aataatgcga actctctatg tagaatcgta
    15901 ccgcatcgat aaccttaaga gcagaaacag aatcagcgat ccatcaacag catccgaatt
    15961 gaaagcgctt ctttcaaaac ctatgtatct ccaccagaaa gatcagacat tcgacgcatt
    16021 aatattagaa gcgcattgtt ctattacgaa ttcttatttt ctaatgacta acaagaatgt
    16081 gactcctgtg cgcatgatcg gcattcccgt cctgccaata aatggactgc gcacaggatt
    16141 ttttgcagct ccgtccccaa atggtacaaa tggtattctt ctctattttg tcacttcagt
    16201 tcaatcgccg tcgattgcac gagatcgagt ctttgaatcg gcctcaaata aagcacttgc
    16261 gcttctgcat tggttcattg agaaagcgtt ttctcaaaaa ctcatcgatc cggttaccct
    16321 cccgtggaat ttcgatgata tgcctgcaga tgttcgcgta tcagcaattc ctggcaagat
    16381 atgatctacc atttcagttg actcaatttg tctttgcaat agaaaccaaa gagccgtact
    16441 cgaaatcttc cgaaaccttg caagtcttga cgaatcaaac taatatcgat atcgttcaga
    16501 acatatatat tcaaaaggag aatctttatg agcattatag aatatatcga agctcgtgag
    16561 atacttgact cacgtggaaa cccgactgtt gaagtggatg tcgtgctcga ggacggtact
    16621 ctaggtcgcg ccgctgtacc atctggggct tcgaccgggg aatatgaagc tgtggaactc
    16681 cgcgatggcg acaagaaacg ctaccagggc aaaggtgttc ttgctgccat tgaaaacgtc
    16741 aacactatca tagcaggcga aatttgcggc ctcgattctc ttgaacaggt cgatatagac
    16801 cgcactctca tagagctcga tggtaccgaa aacaaaggca ggctcggcgc caacgctatc
    16861 cttggcgtct ctatggcaac tgcccgcgcc gctgctgaat acctaggtct tccactatat
    16921 aaatacctcg gtggagtcca cactaacctt ttacccgttc caatgtctaa tatcataaac
    16981 ggtggcaagc atgcggataa cgagatcgat tttcaggaat ttatgatcat gcctcttggt
    17041 gcagaatcat tccgcgaagc tgtccgcatg aatgctgaag tcttccatgc gctaaaatcc
    17101 atattgaaag ccgatggtca taacactgct gttggtgatg agggtggatt tgctccaaac
    17161 atcggcaatg aagacgctct tgattacatc attcgtgcta ttgaaaaagc tggctataag
    17221 cctggtgagc agatagccat cgccctcgat cctgctgcta gcgaactctt cgaggaaggt
    17281 ggcggcaaag gctaccgctt ttggaaatcc agtcccgatc gactcttctc ctctgatgag
    17341 atgattgagc tttacacaaa ctggatcaat aaatacccca tcgtatccat cgaagatggt
    17401 ctcgatcaga acgactggga aggctatgtc aaattcacca aagcactggg caataaaatc
    17461 cagattgttg gcgacgactt cttcgtcaca aataccaagc gcctcgccaa aggcatcgaa
    17521 atgggtgcct gcaactctat tctcatcaaa ctcaatcaga ttggcacagt caccgagaca
    17581 atagagacca tcaacatggc caagcgcgca ggctactctg ctatcgtctc ccatcgttcc
    17641 ggcgagactg aagacacctt tattgcagat ctcgtcgttg gcctagaaac cggccagata
    17701 aaaaccggct caatgtcgcg caccgaccgc gtcgcaaagt acaaccaact aatgcgcatt
    17761 gaagacgaaa tggaaggaat cgcagcgtat tggggtcata aggtcttaaa tgccgtctac
    17821 cgcccataga tcataaatga cttaagcgct ccataaggac tctatctgag agctctaccg
    17881 aagatctcta atttcaagtt cttgtgctcg tggaatcaaa ggaaaggcga aacctccgtc
    17941 cctcgtgcct aatatgtagt aaaggacatc tatcccgaag atgcacccta gtccgagatt
    18001 atcaaaatct ccgtctttcg ctattttgaa ggattatcga aacctccgtc cctcgtggtt
    18061 actcgtggtt acctaatgct tgcatttgga ctagaataaa aaatcatcag atccgccaaa
    18121 gttcatcatt gcgtttgtat ccatatgcaa tgacataggg aggagattct atgctcacac
    18181 gagaccttga ccggataaga gaagcttaca agcgcaggca cccggaagct actcgtcgtg
    18241 catataatcc ctctcatccg caccaagccg aagaaaagca cacaacgact caagataaac
    18301 atatcaaaag cataagatat agcggtttcg atggtactat cacaaccttc actgctgcat
    18361 caggtgttgc atgtgcttct ctgtcttctg gaatagtgct catgataaga cttgcagatc
    18421 tgctcgcaga tggcctatca atgtcgatca gctattacct ctcatctaaa agtgaaacgg
    18481 aatacgaagc tgatagacga gcacgcgatg aatgggaagt tgagaattat cctgaaggag
    18541 aaaaattcga gcttaaagaa atctatatga gcagaggcgt gtcgcaaaaa gatgcaaaac
    18601 ccgcgataga gataaccgca aaaggtaaaa tagcattggt tgatactatg atgattgatg
    18661 agcttgaaat catcctttca gatgaatcac gccttgcaaa cgccatcgcg actttcattt
    18721 ctcttgcctt gtctggatta aatccttttt cgacctatgt ttttgtgctt tttgtgcctt
    18781 ctctcgattc gatccgtttt ttgtttgctt gtatattgat attggcgaga ataattatgt
    18841 tcattctcgg atctttgaag ataattttaa tagaaagaac ttattcaaga gcggctttga
    18901 aatgctgctg gttggatgtg tcgcagcttc tgctacctat ctcgtttgtt tgatcttggc
    18961 ctgactagct taatatgttg ggatcaatta tttaggagga aataaagatg aaaacggcct
    19021 gcatgctact tgctgaagga ttcgaagaaa tcgaagccgt aacgccgatc gattatttgc
    19081 gccgcgcagg catctatgtc aatgtcgtcg gaattgctgg aaagagggtc agaggttcac
    19141 acggcataat catagaaaca gactcaggcc ctgaagcgct ggaaaaggac tatgatgcaa
    19201 ttgtgattcc cggtggcatg cccggcgcaa gcaatctagc aaatagtgct ttggttcgaa
    19261 gcctgataat taggcattat gaaaagggca agctaattgc tgctatctgt gcttcccctg
    19321 ctattgtgct ccatggggcc tgcaatctat tgcaggggag ggaatttacc tgttacccgg
    19381 ggacagagga tcaaattaga ggaggtcact ttcgtgcaga acgagtagtt aaagatggca
    19441 acattatcac ttcacaaggt ccaggcaccg ctggggaatt tgcaatttcc attatttcgg
    19501 tactagaagg accccaaaaa gcgaatgaag tcgcccagca tgcgcttcta aaatgattct
    19561 atattgttac aagaacaaat gaattcgcca tatttcgcgt tgataaacaa aacagagaga
    19621 caaacaaatg ggaatattgt cttctttcgg aattactcca ttttgaaaat gtcctatttg
    19681 gaaaagcaat ttcaaattat aggagctctt atgagttctt ctaccctatt ctaaaggagc
    19741 ctttatggaa aaagaatgca ttcaagcaaa gacagccccc gctgccatcg gtccttatag
    19801 ccatgccatt cgtgcaggtg cccttgtttt ctgttctggc cagcttggca tagatccaag
    19861 tactggcgat cttgtcgaag gcatcgaagc acagacaaat caagcgctta gaaaccttgt
    19921 ctccgtactc gaggcttgca gttgtacggt agccaacctt gtaaaaacca ccatattcct
    19981 caaagacatg aacaatttcc caaaggtcaa tgaaatatac gcaggttatt ttgatggaga
    20041 tttcccagca cgcagtgcaa tacaagtcgc cgctcttcct aaaaatgctc tagttgaaat
    20101 tgaggcgata gcttacaagc aataaggagc cccagaatgt ataatttcga tacaatagta
    20161 gatcgcagca atacctatag cattaagtgg gatagagacc agctgccgga aggctgcaga
    20221 gacgcaatac ctatgtggat cgcggacatg gattttgcct gttctcctga tatagtaaaa
    20281 gcaatccaag cgcgcgcgga acatcccata tatggttaca ccaagtgttc tgaagagtac
    20341 tataagtcct ttattgattg gatgaagcat cgcaatggct ggagcatcga aaaagactgg
    20401 atactatttt caccaggtgt tgtgccagct ataaacctgg ctgttctcgc ttatactcag
    20461 cctggcgata agatcatcat acaaccgcct gtatataatc ctttttctgc tgctgtgctt
    20521 aataattccc gtcaactggt agaaaaccca ctggtcattc gggatggtcg ctataccatg
    20581 gatttcgaag atctcgatcg caagatagat actcagacaa aacttctcat tctatgcagt
    20641 ccacacaacc ccgtaggccg agtatggacc atagatgaac tttctaagct gatcgatatc
    20701 tgctcctctc ataatattct catcatctct gacgaaatac attcggacat tattttgggg
    20761 acagaggttc atcattgcac agccagtctg tcagaaaagg ccgcggccat taccattacc
    20821 ctcaccgcgc ccaataagac cttcaatctt gcaggtctgc agatagccaa tatcgtgatt
    20881 ccgaatcgcg agcttagcaa agcttttgga gagcaagcca cgaagattgg cattaaccta
    20941 accaatacct tcggcatggt ggctcaggaa gctgcgtaca caaagggaga accctggctt
    21001 gaagaactgc tttcatatct caaagagaac tacgccaccc taaagagctt tatcgacagg
    21061 cgcatcccgt ctctcaaggt cttcccgctt gaaggcactt atctgccatg gctagactgt
    21121 cgggtattag gactcagcga cgcagagctc cacgatctat tcctaaaaaa agcaaggctt
    21181 tggcttgatg atgggaccat gtttggcaca ggtggctcgg gatttatgcg catcaatata
    21241 gcctgtccaa gaaacgtgct cataaaggcc cttgaacagc tagaaaatgc aataaagaca
    21301 aacgcatagc ataaagcacc tctttgctat agaatagata tctctttatg ctcacatatg
    21361 tgttggttgg ttttgctcta gcggccgatg catttgcggt ttccgtctct gcagcggctt
    21421 gcgcaactgc tctgcctctt ttgattggca ttcgcgcagc attcatgttc ggcttcttcc
    21481 aattcctgat gccacttgtc ggctggtctc taggttcagc attcagtgct cttatccaga
    21541 attacgatca ctggatagcc tttggactgc tgatcatagt aggcagcaag atgctctatg
    21601 aggctattca tatgcggaga gaatcagctt gtgatgatcc agaaaaagtc gtcgctgcaa
    21661 aaagcaaaac aattgccgct acaaataagg gcacaaactc tgcatcaaaa ggaattatga
    21721 agctaaatac attgctcgta ctttcttttg ccacgagcat cgatgcaatg gctgtggggc
    21781 tctcttataa tcttcttggc atgccgatca tgcttcccag tttcataatc ggtacgatta
    21841 cctttatcgt ttgtataatc ggcatcgaat ttggcaagcg gcttaggaag ttgatccaag
    21901 aatgggcaga aataggagga ggtagcattc taatcattat cggattcaga atcctaattg
    21961 accacatcat aaaagggact taatgtaata ggaaggctta agtctatgga agactattct
    22021 aaagcagtct tctaagagaa acctttagca tcgttcggac ccattctgga ggagatttct
    22081 atgaaagctt cttcaattca tcggtctcat aaatcaattc agtcttacga tgagattcag
    22141 ccaaatgaag tatactgtct tcaaaatcct ggcggcgtga tcttcctttg cacacggggc
    22201 tctgtctctg actctgaaac ctccgtccct catacctccc tccctcaaga ttccgcctct
    22261 cttggcttta gcccacttcc tcatacctcc gtccctcgag attaccgtga tctgcctcag
    22321 cgctatgatt ttgcgccact ctcctggtgc atcccctacg aatatgagcc cgtgagcaaa
    22381 ctgcttgttg tctgcgacac ttcacataag actttccacg acatcgaaga gaatagggtg
    22441 tttgcgattg ccttcccctc attcgagatg cgtgctctta tccagcgtgc aggctcagtg
    22501 agcggattcg atgtcgataa attcgatcat tttaatgtat cttgtttttg tgcaaagacg
    22561 atcgatgtcc taattccaga aggagttgta ggctggatgg aatgctctct cgagcggatc
    22621 attatagaag gaacatcggg tattttgctg ggagccgtca tcaaagggtt tgctatgcct
    22681 gaaagctgga agcgtcggat acattatttg aacgattcaa tctggtacaa accgggcgat
    22741 ttgatctctg attaataggt caaaagtcga atatagaaat atggactaag gatgaaaagc
    22801 aaagaccact aatatggtca aagaacaagg aggttatcga cctatgaaaa aaagactatc
    22861 cattttatcg attgtgttat tgtttgcctc tctgaccacg gctttcgcaa aaccaggcat
    22921 taacttttcc gtgaacagca tcaagaccaa tagcgacata attgcaggaa taccgatacc
    22981 ttccggtgca gatatcggcc ttgaaatccc cattggttct ggcttgtatt cggtgacctt
    23041 ccgaggagca ggtggttacg aatcgagaat gatcttgcgc aacaatgcaa cctctgtccc
    23101 catttctgcc ccatcgtcca tcgacagcct taaccgattc ttttggacca atgctatggc
    23161 agaactaggt gtccggcgct atctggttcg gaaggatgac ggtggcaaaa gcatatggct
    23221 ttttggactc gctcgaggtc gatacgaaga caatgccaca agctttccaa ccacagtatt
    23281 tgctgatgct caaggcatgc aatcgatctc cgcaatgttg ggcattgctc atgattcgac
    23341 tttttggcaa gaacccaatc gaaaggttgg ctggtatgca gaattttcca tggaatacag
    23401 cccaacattt gccaatttca ccacagcgca gatggatttt ggccgcttca atctgaccac
    23461 ggaaggcttt atcccactca cgccagaaac gggaacgctg cgaaatcctt ctatgatggc
    23521 tccctatctt gtgctctatg gcgtaggaga ttatgcagtt ggttcacata ttcctcacca
    23581 aatactgacc actttcggag ggatcaaggg aacgaaaggg cttggtgata tggttagagg
    23641 agctcagcct tggggctatg aagcccctat aaagggctat gcaagtgtgg agttgcgttt
    23701 cccagaccaa tcgatcctag agagttttgc gctatatcct attggatatg tatttggcga
    23761 cataggcgct tatggcggac tatataataa ccctctaggt actactttta tagataattc
    23821 gggagtcatt gcatcagcag gtgcgggtgt ctctttatcg gttcagaatt atctttggct
    23881 tggtgcttat gctggctggc gtttcccgat tatcgatcca ctagcctcga cctattactc
    23941 aaaacccagt ggcttctttt ggggaattac ctttgcagcc cattattgag gcaaacctaa
    24001 gcaccagaaa atcatagacc agaaagggca gagtatcgct gttttcggca gccaaatcag
    24061 ggagagtatg taatgaatat gaataagctc cacctatcga aatctaaaac tcgttacatc
    24121 atagtagttt tccttgcgat actctctctt tccattcatc ctctttgtgc acaaaatcag
    24181 tgctctgtcc ctggtctccc aaccatccgc atcgcaacat ggaatgttaa agactgcagc
    24241 gctaccaatc aaaagacaaa agaagcattt cttttgcacc cttttattgc tgaagccata
    24301 aaggaagcaa gaattgatat cattgccttt caggaaatcc agatcgaggg acgcaaagga
    24361 gcagatatcg cattattgca agatgcattg atgagcatcg actgggctat gccacatgtt
    24421 gcttgggcta gaagcccgct gaatgatgat cttgctatcc tctctcgatt tcctattaca
    24481 gaaagccaaa cgattcttgc gccatccgaa cagccttggc ctcggcctgt cctagaagca
    24541 tgcatcgatt tgggagatat gctcctttac ctatatactg ctcatttcaa ggcttatgct
    24601 gatcaagaat cgctttctgc gcgcctggcc caagctcaag cgctggcttc gcatatccga
    24661 cagtactttg gacaatcgat aaaagaaaca gcgatcattc tagctggcga tttcaacacc
    24721 gtttcgtcct acgacatgca gtccaaaata ggtactgtcg atctgcttca gctgcgagat
    24781 aatgaagatt cgacagacga ttttttgagc ctcaaccttg agtggctcaa tttcaagcca
    24841 acctacaaat cgaaatctta cgcaagcatc acagaccata taattgtctc gccgcttctt
    24901 tcggcctatg ctgatagaga ttcgatcgag attgtcgatc ctcttctgaa cgaaaatggc
    24961 ttatcattat cagaccacaa aatgcttgtg ttaaacctcg accgccgctg gctggtcata
    25021 cagaaatagg ccgaattccc aatatttatg ttgcgctgtc gctatggtct agcatatttg
    25081 ttagaaatca ctagtgcaaa tttaacctag agggcatgaa aatatagcaa agcatatatt
    25141 cttctgttat cgataattgt atcagtaata aggtaattgg catagcaatt gctgagaata
    25201 gaataaactc tgcgactttt agtctacgat tattttaagc cgctgtaatt cgactcaaca
    25261 aataggactt atctatttat gaaaaggtat tctggtaaga gtgaaagcgg atcgtctcga
    25321 aaagagggtc tagtaaaagg tgcatttaga agggctggat aatctcaggc atggcaatta
    25381 atttcttaca taatactatc aagagcaaac atcattggta cacctggtgt tttaaaaatc
    25441 tatccaaaat agaaggaata atgctggcaa gggattcgga cccacgatat gacgcaagga
    25501 taagcctgac ctcctgcctc gcatggcagt atctgcacta ttcctaagtc aattttaatt
    25561 atctaagagc ttttgcatgt caataaacag ctgttttcaa gtatttttaa tcaaattcta
    25621 ctattgttct aattttcact attaaaggat ctattgtgat taattactta tcgttttaac
    25681 acttaaactc gatctgacgg gctcaccaag gattattatt ccccatcatt ctctacagac
    25741 gcaaatgcta aaaagccggt attactccaa aaatgtgaag gttaaagaac tgcttcatta
    25801 actgagcaaa tgttttcccc cctattgtgc aaggatgttt ttacctagta tttcaaggtg
    25861 ttttatcatt cttgccataa agcatacccc tatctcaatg attcagcagt accagtatat
    25921 gttaagtcgg ttatcgggtt tatcgctgtc ctttcgcatt cagtctgctc cgattgaagc
    25981 atttgcagcg agactgctcg ttcccatatc tcccttgcca ataaaatcca gaagacctat
    26041 gtgctatgtt gatgattata tttcctccag attttctgtg ccaagcgaat ctggtacggt
    26101 tcaaaccgag attgccgagc tttgcttccc aattaaacca aatcgaaggc tagtccaaac
    26161 ggtaggggcc tttcagcatc tttgccagaa tggggataac gatccgaaag cctgagcaat
    26221 cttcctccat cgatgcatat ctgtgcggat ccaccaccat cgaggctcac aaactccttg
    26281 actccgcttt ctatgcagat ttgcgcgaac tccgctagcg aagctccaca gctatcaaca
    26341 cctttcctat atgagagctt atttgaacct tcgatccaga ttagtaggag tctaccatct
    26401 tttgatccga gacctaaacg ggctgctcgc cccctttccc aatctagtgg atagacagaa
    26461 ggtggaaaag ttggtcctaa accattccag aagggagaag gcccaaatcc aactgctgcc
    26521 tttccttctc ttacaagcgc tggtccgacc tgtactgcaa acgccacatt agtgaacccc
    26581 aaataatcca catcaaagga atctggataa catctcttat aagtttgaat aatgaatcca
    26641 ccttcgggta tctccgtaca acctccatca tgcaagccta caatatgttt tcctactatt
    26701 atcaaatcgg ttccttcact cttggatgtt ctatcaatag caggccgtga aaaaaatctg
    26761 caattgtaac catcaatata tccggtagat ccgatataca cttccacatc tcttaacgaa
    26821 ggtcggccaa tccagctatt tccagtcttc ccaataaaaa acgcttctcg ctcgaaaaga
    26881 ggtggcaata aaatctttcc atccaagatg agaagcccaa atggctcacc cattatatcc
    26941 caaggagtat cgcagtctgt tgtctccatc tggaagaaat gcgaattgaa cacgatacta
    27001 tttttgctat ggtcaataag ggttcctaaa tctctataaa gcggtaataa gaccggataa
    27061 cccctattaa tcttagctat tccagtcatt cctgcatgcg taagagattc ccaaaaagac
    27121 tcatacttcg agttatcaaa aatgtaattc cctttttcta tcctaaactc acctcgagca
    27181 agtgctttgg taagccaata ttttatgcgc ctctcttgat ttactatgaa accgatctcg
    27241 gtttcagttt gaaatggatc aatagttccc tccggaactg caaacaatat cactggacct
    27301 atcgttgtct tcccaggaag tatattcgct tgaaatagcc tcgttatttc ttttactata
    27361 tcagcatcag tccttccgaa aacccgataa agatgctccc atgattcgaa attgacgatt
    27421 tcatagcgtg caagatctcc attcttatag cgtctttccg atacactaac agtagccttc
    27481 tggctggatt cgctcatcct tttatccccg aatttattga attttcaatt atgtatcgct
    27541 gcaagaatac gaatattagg agaagtggca ttaccgatat aacgccccct gccagaagta
    27601 gattccatct tgcggtatat tgtgactgga actgcgtaag agccatcgtg acggtctgca
    27661 tcgaatcgtc agatatataa aggatgggac gcagcagatc gttccaacta ttcatgaatg
    27721 cgataatgaa cagagtagca gcttgaggtc cactctgtgg aagataaatc cttgtgaaca
    27781 tcctaaaagc gccagcgccg tcgatgattc ctgcgtcaga gatagaagat gggaaagtct
    27841 catagaattc cttaaacata aaggttccga acgaacctac aagaaaagaa ggaataataa
    27901 gcggcaagta actatcaagc caattcagtt tcatcatcaa aaaatattct gggattatcg
    27961 tgacctgggc aggtaccata agggtaaaaa caagcgcaga gaatataagt cttttgcctc
    28021 tgaaattcat cagcgcaaag acatatcccg cgagcgaaca tacgataacc tgaccgatcg
    28081 cagaagcccc ggagataaaa aggctattca gaaaataacg accgaaatgg aactgttgga
    28141 aaattacagc atagttgctc cataattgat ttgtaccgat aagatctggt attaactgcg
    28201 gaggcacgcg aaaaatgcca gcttcgctgc tgaaagagtt tactatcatc caaaagaacg
    28261 ggaacgcagt tagaagggca aaaaagatga gaagtatgta aaggattata tgcgaaacat
    28321 atttcatata tttctctcaa tagtaggaat ttgtaaccca gcgcttctta atatagaaat
    28381 ttgcaatggt aatagctcct acaagcacca tcaaaacata agcgactgct gaagcgtatc
    28441 ccattctgta gtgaacgaat ccatatacat atacatcgta gacgagcgtg gttgttgaac
    28501 tttgtggccc accgcgcgtc attgtgtaga tgagttcgaa gttcttgaat gaatcgatca
    28561 tagctaccgt taaaacgaag aacgtagttg gcgagagcat tggcaaggtg acatgaaaaa
    28621 ataattgcag tgagtttgcc ccatcaacca ctgctgcttc cttcacggaa tctgggatag
    28681 attgcaggcc agcaaggaaa agtaccatat aatatcctgc cattttccaa acttgaatta
    28741 ctgatacggt gagtaaagcc cagtgagtat ctcccagcca attaggaggc ttcagcccca
    28801 cactcttgac aagaatattg agaattccga cttgtgggct taagatccat gaccaaatga
    28861 tacctatagc tactgctgag gttatgactg gtaaatagta agctgcccta aaaaagtttg
    28921 ttcctgggag tttctggttt accattaacg cgagaaaaag gccaattacc ataactccaa
    28981 taacgtatat gatcgagaaa agcacagtgt tcacaacaat cctaataagt ccaggatcag
    29041 acaacgcttc gctaaaatta cttaaaccaa tgaactttgg tgttctgaga agatcccagt
    29101 cggtaaatcc taagtagaaa gaaccgatta tggg
//
LOCUS       DAOMZL010000014        28281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_1329_length_28281_cov_13.250974, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000014 DAOMZL010000000
VERSION     DAOMZL010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 28281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28281
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1329_length_28281_cov_13.250974"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            308..1852
                     /locus_tag="PLQ30_02270"
     CDS             308..1852
                     /locus_tag="PLQ30_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01299.1"
                     /translation="MKRNCKLCLLLLVMFYLCPLIFSQELLEELGQETTIPEKIEEAS
                     SSEVTEFRIRSVKFLIKGHTKERILIEKAQVRIGTTFDSEEDLESFIANIKQRLLNER
                     VLQSAEVECWIEASSENVQEVDLIISVEDSWNIIGLPYFKYDTNEGLLLSIRGRDYNF
                     LGSMQALVLNIDYAMDAEGKQSYGGYTSFGVPFQLLRNDAGLNASQTLKIHADEMPIT
                     SISNLSFWYRYKIDGLYFIFEAAQGLQFNPDEILDDVDPFLLRSSLSLSTSIATGITI
                     PRLGDISYFPSLSFYQLWRPDAVVRSDRQGPRMVFSHGLSFGQIDWIANMRRGLSATI
                     SNTNTYNMQFNDFILDIDGTLCYSATLGGKIGMNGRLSGFYSFTSSAREDLGSSMRGI
                     RNSRLYGISALFLNLEMPIKLFDFPTHIFIKKNWFDFELQFSPFVDLGYVEGVAGNVA
                     ATYPLWYSGGIEFFVYPLRMRTFIVRASLGFDLDAVIQNGSFTHPSPRDSASPYELFF
                     GLGLFL"
     gene            1886..2950
                     /locus_tag="PLQ30_02275"
     CDS             1886..2950
                     /locus_tag="PLQ30_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPW01300.1"
                     /translation="MKQKLFTVGPVEMYPTTLKVGGEQLPYFRTQEFSDLVLGCERSI
                     LSLADAPQDSRAIFLAASGTGAMEAAVINSLTSSDRALIVVGGSFGERFCEICDDAEI
                     PYDAIRLDPGTSLNPAILKSYDLGSYKALLVNAHETSTGVLYDIPRLGEVCRKYGLLF
                     IVDAISAFLCDALSMKSMGIDMLITSSQKALALAPGLSIIVLSARAINIIEQAHVHSH
                     YFGLKKYLLDGTRGQTPFTPAVGVLLQLSDRLRSLEKLGTYALVEQSAARANYFRNLV
                     KDLPYKFFADHPSNALTALSPQNGHSAYDIYEILKDKYGFVVTPNGGKLKDIVFRVGH
                     MGNITYEDLNALAVAIKEIV"
     gene            2950..3654
                     /locus_tag="PLQ30_02280"
     CDS             2950..3654
                     /locus_tag="PLQ30_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HPW01301.1"
                     /translation="MKAILMAAGKGTRISRHIDGKPKCTVDLGDGKTLIQYSVELLGK
                     RGIKDITIVLGYQGEVIREIVGDSVHYVENPFFDITNSIVSLWFAQDYIVGDDRYLIM
                     NGDVFLSEEALDRIVVEKLSPVLFYDSTRISEADYRFYCKDDRLMKFGKDLSNEETSG
                     EYIGCAIIGDGFATTFIQRMNELIHTQHHSQWWEDVLYSMAEEQALAVKDIGGAFWAE
                     VDYVEDYLRIQDFYRK"
     gene            3656..4426
                     /locus_tag="PLQ30_02285"
     CDS             3656..4426
                     /locus_tag="PLQ30_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01302.1"
                     /translation="MNWLVKIFAIVLVLCSILPFFAAASELVPEIMFQKLTQEETAML
                     KNGEPIIRSIRSSSDMLLVPFDKDSQMLIERAKKLNGNYITEFILALPLTRKGQGTMI
                     LDRLLKVIANVKGYVSIPYYSVRQQTTYDLFDKMDIISQTKFSEGESIVVLQHMEPFD
                     DFRAEYRYAKIGNNLSFSGENLDSIIYSYRNLSVVAPHKMRWELYAFEKDGCLYAYGY
                     GAVRAFDLFGLFRDRLEPSFMGRVSAFFKYIAEKMSLS"
     gene            4459..5544
                     /gene="gmd"
                     /locus_tag="PLQ30_02290"
     CDS             4459..5544
                     /gene="gmd"
                     /locus_tag="PLQ30_02290"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPW01303.1"
                     /translation="MKTALITGITGQDGKYLAELLLEKGYMVHGIKRRSSSFNTQRID
                     HIYTDPHAPECKFRLHYGDLTDGTNLIRLIEELQPDEIYNLAAQSHVKVSFEVPEYTA
                     NVDAIGTLRLLEAIRLLGFEKKTRFYQASTSELFGKAQETPQRETTPFYPRSPYAVAK
                     LYAYWITINYREAYGLHASNGILFNHESPVRGETFVTRKITRAAARIKYGLDERLYLG
                     NLDAQRDWGYAKEYVEGMWLMLQQEMPGDYVLATGRTSTIRAFVEKAFKRAGFSIAWK
                     GKGLEEKGIDTVTDRILVEVDPQYFRPSEVDSLLGDASKAREKLKWQPHCTLEELIDI
                     MMEHDLAEANRESHLKEHGFPVANPHE"
     gene            5554..6618
                     /locus_tag="PLQ30_02295"
     CDS             5554..6618
                     /locus_tag="PLQ30_02295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPW01304.1"
                     /translation="MKLLFVIQDSHGWAGTERVTNLIANGLSDRYEIEVLSLSERPSC
                     EKGYLYKASIVRSYIPIHQGVLGLFSINTKTARHISRGAYDVVVISGVGEIKFLLVAI
                     LFCRTKFVAWEHFNAAYTHRRVNRKVAARFCDAIVVLTRADADDWQLYLHPRGHIVRI
                     PNPLATIPRQPSLLTTTRVLALGRLEEQKRFDLLIDAFVLMHAKYPDWTLRIRGTGSK
                     EAELKNRVKELGLENAIEILPAVEDVASEYQVASIYALSSKFEGFPMTLIEAMAFGIP
                     CVSFDCPNGPSEIIAQGEDGFLVPYGDVTALAGALAKLAQEPTLRMQMGRKAREHIKR
                     FDQEHVSALWEDFLAKLCQE"
     gene            6623..7399
                     /locus_tag="PLQ30_02300"
     CDS             6623..7399
                     /locus_tag="PLQ30_02300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPW01305.1"
                     /translation="MPTVSVVIPVYNAMATLGRAIESVLSQNSIGNTIEILIVDDGST
                     DQTGTIATQYVQAYPDRIRLIHQHNQGPAATRNTGIAAAVGELIAFLDADDWWLPGKL
                     ASQLRVLEEKPEVALVCTTMNGHSLWGKPAVIILRFRDLLWSNKVYTSSVLARKTILL
                     AAGCFNPQRRLCEDYELWLSIGATHPIALLNTPYLMYSKGIGTSSRLWAMERAELKTY
                     AILKQRKLVSGPIYTILRYWSLAKYMIRVVQKFALGKHFI"
     gene            7517..8461
                     /locus_tag="PLQ30_02305"
     CDS             7517..8461
                     /locus_tag="PLQ30_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HPW01306.1"
                     /translation="MEKHSTIYIAGHRGLVGSAIVRCLTKNGYDNLIVRTRQELDLAR
                     QADVEAFFHTVRPKYVFLAAAKVGGIGANSTWPAEFIYQNLAIASNVINAAYRSGVKK
                     LLNLGSSCIYPKLAPQPLKEEYLLTGPLEPTNEAYAIAKIAAIKLCKFYNEQYGTNFI
                     SAMPTNLYGEGDTYDLFSSHVVPAMIRKFHEAKKSRDPVVLWGDGSPYREFLYADDCA
                     DALVFLMEQCDYSNIGEIINVGSGFEETIRELATLIASVVGYKGKIEWDISKPNGTPR
                     KLLDSSKLFSYGWRPSVGLREGIERTYSDFLNRKKEII"
     gene            8464..9318
                     /locus_tag="PLQ30_02310"
     CDS             8464..9318
                     /locus_tag="PLQ30_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW01307.1"
                     /translation="MANGNDETWDVIIEAKKNLLSFDIKELLRYKDLIKLLIRRDFVS
                     VYKQTIFGPLWFLFQPLMSTLIYMFVFGNIAQLGTDGIPQPLFYFSGTMLWTFFATNL
                     QKSSDTFVNNTGLFGKVYFPRFAMPISYMVNALFTLGIQFAFMLIFYIYYIATGYHFV
                     PTGWILATPLYIIQLAMLGMGFGLLTSALTTKYHDLRNLVNFGLSILMYATPVVYPIS
                     NVPVKWKLLFQINPVTPVIEMFRYSFLGNGNHDLRMWLYSLVASLVIFWIGLVTFTYN
                     EKTFIDVI"
     gene            9325..10053
                     /locus_tag="PLQ30_02315"
                     /pseudo
     CDS             9325..10053
                     /locus_tag="PLQ30_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            10109..11041
                     /locus_tag="PLQ30_02320"
     CDS             10109..11041
                     /locus_tag="PLQ30_02320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW01308.1"
                     /translation="MDETEYTKIILSIIVPVYNSAKFIRATLSSIIKIIDRRSDIEVI
                     VQDSCSTDGTSNIISSFSEKYPLIHHFIEKDNGQTDGINKGALKAKGLWITWLCGDDL
                     LMNTFRKSIEILPQVEADVVYADCVFLLENGNAIPAIGTEAYNNGILAKRRLIMQQPG
                     TCIRKRLWDIIGGVNNNLNWVMDYDLFLRLETKGARFLRIEEFVAISRIHSEAKTSSR
                     SIERLFEHWRILFNAHIHKIIYFSLRPYLLYFVEYIIKNRESQSKFKGITYLHKLFWL
                     VGYPAEKELIESRFEKKKTEIEAEIRVLDKGIAI"
     gene            11038..11919
                     /locus_tag="PLQ30_02325"
     CDS             11038..11919
                     /locus_tag="PLQ30_02325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPW01309.1"
                     /translation="MSQGSLYVIIPTTRGEKAFPSIYSALSQRGFENVFVLICGKSAN
                     NQEFANQCLNKFNSRVIVLHCPAKEHILPGEARNIGIEYVVRYANPSDYILFLDDDIC
                     IPPNYAIDLKNFISCHGLAAAMGNMTSFPINIWSKIIDYSNFWWLQCNRDCLNRGWLG
                     AGATILKISDISSIRYLSDIAINEDIDFFDRISTLTTKKLGICSSIRSRHFHNRSSLI
                     GLIKYQFLNGYRGPIYHLTRKRVILQSLKRWVSFSKVAIKENINYLKYHIVELIGVIF
                     SFLIVECGAVLSTLRRR"
     gene            11923..13065
                     /locus_tag="PLQ30_02330"
     CDS             11923..13065
                     /locus_tag="PLQ30_02330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW01310.1"
                     /translation="MKGKIVIIGHSYHLKTKSNEFFLDLLNKHYDVEFIADESWEKNV
                     SPTLEYIDETYRSVIFWQMISLNCLKRIRCKNIIFVPMFDQSGGAPKSFWYHLRNVKI
                     ISFCKTLGDILTGLGFNVLNVTYFPEPEEIPINVEPGTCFFWQRTNMIDWPKVKRLLE
                     KSDVKKVHIHTAVDPGHRFVKPSKADEKSYHITYSSWFKTRNEYLKLVDKSQIYIAPR
                     YQEGIGLSFLEAMARGKVIIAANQPTMNEYINNGFNGYLFDPNDNKPIIFDNIDQVKQ
                     NSIQTIKVGYEKWIDDQDRIIDFIEAPSSRNFYFRKHPFSRYDYEAMGNAIKIVIKYI
                     LPFGIVKVIWYFKKDKRWKKILKEAIKIFIPYGIIKLVSYFRAKKN"
     gene            13088..13831
                     /locus_tag="PLQ30_02335"
     CDS             13088..13831
                     /locus_tag="PLQ30_02335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW01311.1"
                     /translation="MDKVLFSIITATYNVKGQDIEATKESLLTQGTNNFEWIIIDGST
                     NEQSLSQICHSIQGIPIRTTLLAERDEGIFDAFNKGIKLAKGEWIAFLGCGDIYYENI
                     LMRIEDKLRTIYDYDILYGITDIIRENGAHYWFSRSEEYLIEGEMMHHSACFVRRSAY
                     EQIGGFDCSYISASDLDALIRLYQRGKKFYFLNMIITAFHVGGISTKGRLPYEEKMKI
                     MKKYGYISKKEQVKYVIKAIINKAKNVFC"
     gene            13843..15084
                     /locus_tag="PLQ30_02340"
     CDS             13843..15084
                     /locus_tag="PLQ30_02340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPW01312.1"
                     /translation="MYTIKIYCPNIISGYGGAEGYVLTLASYLSNTYKNIDLSMVIYA
                     TTECKECANHENDRPMKNQMREELSKKYNLAFSDELDFIILPESKNNSVISKYCAHTR
                     LRGTSTGTDLYINGFHNVHYFHGRKNIHIIHFPAPPRVQASPFFTKHRFFKPVAAALD
                     SRYRNCYDLFLCNSAFTKKWLEHYWHVNEDLIQILYPPVLILDEKRDSQNRDSINQKE
                     KNILMVSRFDPRKKILELVDFFIENNAHYPEWRLLVAGSTDRRYQSYLDVIEKRAQNN
                     NIYIYKDIAPIELACLYQKSSIFWHAMGLDVDEEKDPLDLEHFGITTVEAMSAGVVPI
                     VINKGGQKEIVDHGINGYRWNNLKELRMYTRAVMLDETLRQSLARAAIEKSKIFSRES
                     FYQHADEIFQRFSLIPEEYRK"
     gene            15084..15983
                     /locus_tag="PLQ30_02345"
     CDS             15084..15983
                     /locus_tag="PLQ30_02345"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HPW01313.1"
                     /translation="MLAYQRDFDEKFPDVRPAKTSNIRQGQMVMLRLLKIFHAACQEL
                     GLTYWLCGGSLIGAIRHGGFIPWDDDVDVFMPREHYQEFKQQASEVLPYDVYLQTDTS
                     WMRLVDRFGSKEIEEGNDFIFIDIFPAKRFPLGRKMLRKVRMVIPPYPLPGIPKDASF
                     ARQLKRRAIHYGAIFLRVTGLSYVIRFLCTLGPRRYWSYDLEVTWRFYYRDDWIFPLH
                     LHTFEDTAFFVPCDPHKVLQHQFGDYMTPPPENQRINHGHKAFHVTQSNGHPESLEWL
                     QFAEKKKQAAEKAGWLDSCTPSS"
     gene            15965..16744
                     /locus_tag="PLQ30_02350"
     CDS             15965..16744
                     /locus_tag="PLQ30_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HPW01314.1"
                     /translation="MHTFVISLPEDIARRASIETQLEQLGIEFEFFDAVRGSEKISDP
                     RWYDDAAARKLEGRSLKPGEVGCALSHAAVYTEIAQRGLPWALILEDDAALAPDLPQV
                     LSLLERKYLEQGDLVVLSRCDFYLPWTRKKVTDRYQMVTPIFIKEGSIAQTVGYIITQ
                     EAAVQIRNINIPVKFPADSWGYYRHHVRFKGIIPSLSLVTQHVELGSTITGNGQRTSF
                     QRYSLQGILWNSFKTYNPLGRKLKYYAKQWQNRHGKSPTGI"
     gene            16719..17705
                     /locus_tag="PLQ30_02355"
     CDS             16719..17705
                     /locus_tag="PLQ30_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01315.1"
                     /translation="MGSPQQAFELLLLAIERDLPVLSVGLPFIIHFLMPQKITIIGSC
                     HCLEKARSIGLDESFDKSSDKSSGAIIEYLDEDAVVQGLSLAAVKACIQAHGGKGERA
                     GWYFKQLLLLAYATRQSPYNYYLVWDTDTIPVRPMTFFDAAGRILMTTKTEVHPAYFR
                     TMEKLIGIGKVVSGSFIAEHMMFERPYVSRLLVCITKNKARDESFDAQGFIESVIAAI
                     EPEDLDGSGFSEYETYGSFMYRTAGERIALRDLPSMRLGSSFFDRPPTTKQLFALSRH
                     YYWASFETWHGTTLKTRIAYQVRKAIGALWTTGSVLLAPKAFDRFQKEIAHE"
     gene            17698..18573
                     /locus_tag="PLQ30_02360"
     CDS             17698..18573
                     /locus_tag="PLQ30_02360"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HPW01316.1"
                     /translation="MNNSLRYLSFPPDNRLSAPDPLRGCQLVMLRLLRIFDAICKELG
                     LCYWLDSGTLLGAVRHRGFIPWDDDLDVAMVDHDYQEFLHKAPFYLPADVFLQHRESD
                     PHYDLPFAKLVDRYSLLDSPDGKKDGALSGIFIDIQPMSFFTYRQKAARKVLYVLHGD
                     YGKPDASRGVQRNVKRAILATFRTIALITSLDHALDSFCAYGAPKWLAYDLSFQRWWP
                     IFHSIDSVFPLQTIQFEGYTFSVPHEIDVYLRAQFGDYMTLPPLEERRPVHSGGIHLT
                     GPNPHPLGLNWKDHP"
     gene            18570..19475
                     /locus_tag="PLQ30_02365"
     CDS             18570..19475
                     /locus_tag="PLQ30_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-fucosyltransferase"
                     /protein_id="HPW01317.1"
                     /translation="MKIVKVIGGLGNQLFQYAFSRALSLKTGDEVLLDISSYTDTQRQ
                     IHYGFELPQLFPIRYKVADELDIKQLSTQPRSPLSQLRRRYFRKRTHYIDKYFCFNPE
                     VFELKGDWYLEGWWQDERYFAWCSDILRKELSFIADPGARNRDLLAEIQASHHGSIPV
                     SLHVRRGDALKNPDTRVSTTAYYNHAIEAVRSLVAHPYFLIFSDDLLWCTGNLALDPS
                     EAIYIDWNRGCDSWRDMWLMTKCRCHIIANSTFSWWAAWLDERPDKIVYAPERWSLAR
                     ARCFEYYHYTFDQVVPPSWIRIPIE"
     gene            19507..20424
                     /locus_tag="PLQ30_02370"
     CDS             19507..20424
                     /locus_tag="PLQ30_02370"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HPW01318.1"
                     /translation="MFPDFDTLIPDLRPEGDTPLRSCQLVMLRLLKVFDYICRKEGLR
                     YWLDGGTLLGAVRHGGFIPWDDDVDVKMPIEDYRRFLNLAPQALPYDIFLQTKSTDPA
                     HTAPWAKLRDRFSFMNDPGGPFAYSQGIPIDIFPAVYMTMRQHKWRKFFCLMPPYSMP
                     PQWPVRTWSLPHKLYSGTFAILQTIAKPILSIKALARALQHWSDKGEKVWTNDYPIEW
                     MREAFPDEVVFPLSQIRFEDAMFLCPADPDRYLRIFYGDDYMTPPPENKRGAHRVDGF
                     LITGPNPHFSGLCWEDYEEKKRRVAAKLP"
     gene            20421..21530
                     /locus_tag="PLQ30_02375"
     CDS             20421..21530
                     /locus_tag="PLQ30_02375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW01319.1"
                     /translation="MRILHIVTNSDLGGAPRVVTELSARAAKDGHTCAVAAAPDGPMW
                     RYLDVHVQRFPVYSIRREIDPLEDLKTFCSLQKLYGSFRPDIIHLHSSKAGVLGRLSA
                     LSWGIKYTRRCVYTIHGFDTILKAHNRYLPLERMLARATGAVVPVSHYDERNLRAAGI
                     CKRVVMIPNGVTNRRGMPSPDLRAASCIAEAKAQGKPIVLTIARLESPKRPDLFVDIA
                     RKITKASFFWVGNVQNPTSMLPAGTKIPENLFFLGESPEAGTLANLCDIFMLLSDYEG
                     APMSILEAMSCAKPVVASNVGGIPEILGEGSDAAGILVPNKPNIISNAVLSIIEKPSI
                     ANRLGAMARQKFESSYSADHMWESYLDLYKKLSKL"
     gene            21584..22951
                     /locus_tag="PLQ30_02380"
     CDS             21584..22951
                     /locus_tag="PLQ30_02380"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPW01320.1"
                     /translation="MHKSYRHLQLSIFFVDVLLMYCALILALLLRNKSFPAPWSLRIH
                     LFHFSIIFVGWVIVFYTLGMYRLERPFDDLVFLQRVLLGSLISGLASAGYFYLAPSVP
                     IEPKTVLFLFVVLYTVMFWCWRYGLAKLRRLQSLRVGVGFIELQPTSFEIIYETLARS
                     PLGYDIRFVYNPPPDIQLPESIRRVLHEEDLRVVIEETDTDLIILGENRGPSGEMLRA
                     LFALLDRRVRFMRLSAFYELVVRRVPVGLIDESWFLENVDLKAKKPYEIVKRGIDIIM
                     AVFLLVVTSPFWLLIALAIKATSKGPVFFRQTRLGRFELPFTILKFRTMRTDGNGHEP
                     TQVDDPRITPVGSFLRSTRLDELPQLLNILKGDMSFIGPRPERPDIAYTLGKEIPYYR
                     QRHLVKPGVTGWDQVSGQYHSCSVEDTYKKLQYDLYYLKNMSLYLDVSIFFKTIITVL
                     KREGR"
     gene            23032..24390
                     /locus_tag="PLQ30_02385"
     CDS             23032..24390
                     /locus_tag="PLQ30_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01321.1"
                     /translation="MSALDFTVGASGAYSTESRKNFFVDGSIALHHSEGPFTLSWELT
                     ADSKGTYPESFLADFFGPFSLDIKNAGFTYMARPLSFFLGKLPLKDEINSPYSLFISG
                     ASPSVMTGGFRFDSGIFSFSDQWIGLDHNVKNNLYKTTEAGIYADRGIVLKTYSLDVG
                     ALKLGYQDVVLFTGSYFDVDIFAIPAPSALLQDILTASGRPSSRSGDMNSIMGFFGEY
                     RNGGLKLCAQILIDDFNMNRFIAPSAYQNPDKIAWSLGSEIDLSLGKLSLYTAGATKY
                     TFESVREEFYSYTLHAGSAIYSDSQLIAVPLENQMLGYINGENNLAFMANWSAPIAQL
                     DLETGLECVISGSKAPTNPWHNGEDWASLGTQLLNDPALEVKLLLNLRISRKWGDFTL
                     FMQTTGGYVFNQLNPVYPGESGYPILTYSDGDMLEPVFVPKVGDSAPIAEIILGGIWS
                     LY"
     gene            24416..25594
                     /locus_tag="PLQ30_02390"
     CDS             24416..25594
                     /locus_tag="PLQ30_02390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW01322.1"
                     /translation="MRIAFFADAYRPRVSGQVASLDEFCRSLTERGHEVCIVCPEYPP
                     ERMKGQSEPFKTIRVPSGWAIVSADDRLGLPWKGQRAMRELDEFDPEAVHIQTEFSIG
                     AMGRRYCRKRGLPIMSTCHTFYEMYVKWYLPFLPDFVARPVVKTWMRSVYAHDELIVI
                     PSRRIRDIMLSYGIDKEYAVIPTGVDERIFYSQTEKGKAYRQKLDELWPGVAEGSLLV
                     YVGRISLEKNLEMLADAIRRVFAEDPNAYLAMVGDGPRKAEIQQMFLCKGLKDRVIWT
                     GFVPREELPIVYSAADLFVFPSVTETQGLVTIEAMLCGTPVVGVNKMGTAEIMEGDTG
                     GLLAEDDARDFARQILRLLGDPSLRAEKASQALKHGQNWTIGHLCDEMEKLYLRIFGS
                     "
     gene            25554..27572
                     /locus_tag="PLQ30_02395"
     CDS             25554..27572
                     /locus_tag="PLQ30_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01323.1"
                     /translation="MKWKSSTCAFLDPNGDKRTLERGGKILAAMRFLLMRLFIMRFLF
                     KKTFEVQLHFAMVFCAVLILCAMQNKAYAQEVTLWNPTTVETSSQGSGEAQKSIHHPA
                     DLFFPGDEAYDTAVLEAIRAGHVPESNVFPLFGSEFDVVSAPKKAPGGNSEINSSSNS
                     ESSLGSTCAAPVWRSGFSLSLSTPTLYYDSPATQDDLASNLLGEPSLTVNAFPEIQHF
                     WNTLPDVLALDIRLYPSRSIAFKIDASLFYRRDKIIDQNSTFQWNLDEWLGNLLGVAE
                     FPRKSYVAWVGKGYGVALGRFPSGMGWGKLSGSILNPRASWYDQIRFHLDSGPFRFTS
                     MIATSSAQLSKAEEEIQFRKKEDGSSFWDSQNDHDYAGHDQAIKLANWHQIEWKPAPW
                     LEIALAEMSIMGGRLPSLSFVLPSAIWHNVYAAGYSNVGVSLSAAAVPLPGLLISGEL
                     FVDDVLSYDEAGTTKPQALAWLGTARWSNALTDKLFLDSGLEYQHVDQWTYTRWNPYL
                     AMYQRQALTGGRRSLDQSLGAPWGPDYDSIGAYANLRFRSGASLQLSYEFIRKGPIYQ
                     GMASQVNVTADFDGNGKIDQMPQTIWVPVYYDYDKYAGDGALEAILSRPDEYRHLVTL
                     TGALPLGEAVDFKLSATFGFYQNFGHISGATDTAFLLYAGIVVRLASR"
     gene            complement(27678..28244)
                     /locus_tag="PLQ30_02400"
                     /pseudo
     CDS             complement(27678..28244)
                     /locus_tag="PLQ30_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454862.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         7780 a         6082 c         6332 g         8087 t
ORIGIN      
        1 gctcatcaca attctgtcca gaaaagatgc agcaaaaagg gcagagcaat gattcctata
       61 caagaaatat tatgttactc tcgaattctt gaacgcattg ctgcataatt aaagagacta
      121 tgatatattc atttatttgg caggtcggta caaaagactg gctaaaatcc gcatctgtaa
      181 aggttgggct tttttagcta tcaaatctta ttttgcttga ttgcatggat tttgcgtttt
      241 ttgcccgcaa agatggctta gctgcgcaat aatatgacta gagcgatagg aaaatgagga
      301 gaagataatg aagcgcaatt gcaagctatg cttattgctc ttggttatgt tttatttatg
      361 tccactaatt ttttctcaag agcttttaga agaattaggg caggaaacta cgattcctga
      421 aaaaattgaa gaagcatcat cctcagaagt cactgaattt cgcattcgat cggtaaaatt
      481 tcttatcaaa ggtcatacta aagagagaat actaatagag aaagcgcaag taagaatagg
      541 tactacattt gatagcgagg aagatcttga gagttttatc gctaacataa agcagcgatt
      601 gcttaatgaa agagtgcttc aatcagcaga agtcgaatgt tggatagaag cttctagcga
      661 aaacgttcaa gaagttgatc ttattatttc tgtcgaagac tcttggaaca tcattggtct
      721 tccttatttc aaatacgata ctaatgaagg tctgcttttg agcattcgtg ggcgagacta
      781 taattttctt ggctccatgc aagctcttgt cctcaatatt gactatgcaa tggatgctga
      841 agggaagcaa tcgtatggtg gatatacctc ttttggtgtt ccatttcagc ttcttaggaa
      901 tgatgctggc ctaaatgctt cccaaacgct gaaaatccat gcagatgaaa tgccgatcac
      961 ttctattagc aaccttagtt tttggtaccg ctacaaaatt gatggattat attttatttt
     1021 tgaagctgct caaggccttc aattcaatcc agacgaaatc ctcgatgatg tagatccatt
     1081 tttgcttcgt tcatctctct ccctttcaac atccatcgca actggcatca caattccaag
     1141 gcttggggat ataagctatt ttccttctct gtctttctac cagctctggc ggccagatgc
     1201 agtagttaga tcagaccgcc aaggtccgcg aatggtcttt tcacatggtc tctcttttgg
     1261 tcagatagat tggattgcta atatgaggcg aggattatca gcaactattt caaatacaaa
     1321 tacatataat atgcaattca atgattttat tttagatatc gatgggacac tatgctatag
     1381 tgcaactctt gggggcaaaa taggcatgaa tggacgcctt tctggtttct atagctttac
     1441 ttcatcagcg agagaagatc ttggctcatc aatgcgtggc ataagaaact cacgacttta
     1501 tggcatcagt gctctatttc ttaatctcga gatgcccatc aaactattcg attttccaac
     1561 gcatatattc ataaaaaaga attggtttga tttcgagctt cagttctctc cctttgtgga
     1621 tcttggatat gttgagggag ttgcaggaaa cgtagcagca acctatcctt tatggtattc
     1681 aggaggcatt gaattctttg tatatcctct tcgtatgcga accttcatag ttcgtgcaag
     1741 ccttggcttc gatctcgatg cggtaataca aaatggctca tttacacatc ctagtccaag
     1801 agacagcgct tccccctatg agctattctt tggacttgga ttatttttat aagatccaat
     1861 tatgtgggca ataaaggata tgcttatgaa gcaaaagctt tttacagtag ggcctgtgga
     1921 aatgtatccc actaccctga aagtaggtgg tgagcaatta ccatatttcc gcacacagga
     1981 attctccgat cttgtgctcg gatgtgagcg atcaatatta tctcttgctg atgctcctca
     2041 agacagcaga gccatctttc tcgcggcatc aggaacaggc gctatggaag cagcagtcat
     2101 caattcactt acttcctcag atcgcgcttt gattgtggtg ggcggatctt tcggagagcg
     2161 attttgcgaa atctgtgatg atgctgaaat cccttatgat gcaatcaggt tagatccagg
     2221 cacttctttg aacccagcta ttctgaaatc ctatgattta ggtagctata aagctttgct
     2281 cgtcaatgct cacgagactt ctacgggagt gctctatgac atcccgagat taggcgaagt
     2341 atgccgaaaa tacggtttgc tttttatagt tgatgcgata agcgcattcc tttgcgatgc
     2401 tctctctatg aaatctatgg gaatagatat gcttattaca agctctcaga aagctcttgc
     2461 gctcgcacct ggtttatcga ttattgtttt atctgcgcgc gcaataaata ttatagaaca
     2521 agcccatgtg cattctcatt attttggtct gaaaaaatat ttgctcgatg gaacaagggg
     2581 gcaaacgccc ttcacacctg ctgttggagt tttgcttcag ctttcagatc gattacgatc
     2641 tcttgaaaag ctaggtactt atgccctggt ggaacaaagc gcagcacgtg caaactattt
     2701 tagaaatcta gtaaaagatc ttccctataa attttttgca gaccatcctt ccaatgcgct
     2761 cacagccttg agtcctcaga atgggcattc tgcctatgat atctatgaaa tcttaaaaga
     2821 caaatatggc tttgtagtca cgccaaatgg aggaaaattg aaagatattg tatttagagt
     2881 aggacatatg gggaatatta cttatgaaga tctcaatgct cttgcagtag cgatcaagga
     2941 gatcgtctaa tgaaagcaat tctcatggct gctggcaaag gcactagaat aagccgccat
     3001 attgatggga aaccaaaatg cacagtcgat cttggcgatg gaaagacgct catccaatat
     3061 agtgttgagc ttctagggaa gagggggata aaagacatca ccattgtgtt aggatatcag
     3121 ggagaagtga tcagggagat tgtcggggac agcgtccatt atgtagagaa tcctttcttt
     3181 gatattacca acagcatagt ttctctttgg ttcgcgcagg actatattgt tggagatgat
     3241 cgatacctga ttatgaatgg tgatgtcttt ctttcagaag aagctctcga tcgaattgta
     3301 gtcgaaaagt tgagccctgt tctcttttac gattcgacaa ggatatcaga agctgattat
     3361 agattctatt gtaaagatga tagattgatg aaatttggta aagatcttag caatgaggaa
     3421 acatcaggag aatatatagg atgcgccatt attggtgatg gatttgcgac cacttttatc
     3481 cagagaatga atgagctcat ccacactcag caccacagcc aatggtggga agatgtactg
     3541 tattcgatgg ctgaagaaca agctcttgct gtcaaggata taggtggcgc cttttgggca
     3601 gaagtggatt acgttgagga ctatcttagg atacaagatt tttataggaa atgatgtgaa
     3661 ttggctagtg aagatttttg ccatagtact tgttttatgt tctatattgc ctttttttgc
     3721 tgctgcttca gaattagttc ctgaaataat gtttcaaaaa cttactcaag aagaaactgc
     3781 gatgttgaag aatggagagc ccataattag gtctattcgc tcttcttcag atatgctgct
     3841 agtgcctttt gataaggatt cccagatgct gattgaacgc gcgaagaagc tgaacgggaa
     3901 ttatattact gaatttatcc ttgctcttcc tttgaccaga aaaggtcaag gcacaatgat
     3961 ccttgataga ttgttgaaag taatcgcaaa tgtaaaagga tatgtgagta ttccttatta
     4021 ttcagtcaga cagcaaacta cttatgattt gttcgataag atggatatca tttcgcagac
     4081 gaaattttct gaaggcgaaa gtattgtcgt gcttcagcat atggaaccat ttgacgattt
     4141 tcgtgcggaa tatcgctatg caaaaatcgg aaataatctc tccttttctg gagaaaattt
     4201 ggattccata atttattcat ataggaatct ttctgtggta gctcctcata agatgcgctg
     4261 ggagctttat gcttttgaaa aggatggctg cctctatgcc tatggctatg gcgcagtgcg
     4321 ggcattcgat ttatttggtc tattccgcga caggcttgag ccttcattta tggggcgtgt
     4381 aagtgcattt ttcaagtata tagccgaaaa aatgtcacta tcctaaagat ctataggaat
     4441 cgctgaagga gttgacttat gaagacagcg cttattaccg gtattaccgg ccaagatggt
     4501 aaatacctcg ctgaattgct tcttgaaaaa ggctatatgg ttcatggaat caagcgaagg
     4561 agttcgagct tcaatacgca acgcattgac catatctata ctgatcctca tgctccagag
     4621 tgcaaattca gacttcatta cggtgatctc actgatggca ccaacctcat tcgcttaatc
     4681 gaagaactac agcctgatga aatatacaac cttgcagccc agagccatgt aaaggtctct
     4741 ttcgaggtcc ccgaatatac agccaatgta gatgcaatcg gtaccttgag gcttcttgag
     4801 gccattcgcc ttttgggatt tgagaaaaaa acacgatttt atcaagcttc tacctcggaa
     4861 ctctttggca aagcccagga gactcctcag cgcgaaacaa ctcccttcta tccaagaagc
     4921 ccttatgcgg tcgccaagct ctacgcctat tggatcacca taaactaccg agaagcctac
     4981 ggtcttcatg ccagtaacgg tattctcttt aaccatgagt cgccagttcg aggtgagacc
     5041 tttgtgactc gtaagatcac tagggcagca gcacgcataa aatacggact cgatgaaagg
     5101 ctttatcttg gaaatctcga tgctcagcgc gactgggggt atgccaagga atatgttgaa
     5161 ggaatgtggc taatgctcca gcaggaaatg ccaggtgatt atgttctggc gacaggccga
     5221 acctcaacaa tacgagcatt tgtagaaaag gcattcaaac gagcaggctt tagtatagca
     5281 tggaaaggca aaggccttga agaaaaaggg atagacacag taacagatcg cattcttgtt
     5341 gaggtcgatc ctcaatactt taggcctagc gaagtagact cgcttcttgg tgatgcatcc
     5401 aaagcgcgag aaaagctgaa atggcagcct cattgtacct tggaagaact tatcgatatc
     5461 atgatggaac atgatctcgc tgaagctaat agagaaagcc atctcaaaga gcatggtttc
     5521 ccagtggcaa accctcatga ataaggcttt tctgtgaagc tgctctttgt cattcaagat
     5581 tcccatggtt gggctggaac ggagcgagtc acaaacctta tcgccaatgg cctctcagat
     5641 cgatatgaga tagaagtgct ctccctcagc gagaggcctt cgtgcgaaaa agggtatctg
     5701 tataaagcct ctatagtgcg ttcctatatc cctattcatc aaggagtttt aggtcttttt
     5761 tctatcaata caaaaaccgc tcggcatatt agccggggcg cgtacgatgt ggtcgtgatt
     5821 tcgggggtag gagagatcaa gtttcttctc gttgctatcc tcttctgcag aacgaaattc
     5881 gttgcttggg agcacttcaa tgcggcatat acacatagac gagtaaaccg caaggttgca
     5941 gcacgcttct gcgatgcaat agtcgtgctc accagagccg atgctgacga ctggcagctc
     6001 tatctacacc ctcgcggtca tattgtcagg attcctaatc cgctagcgac aatacctcgg
     6061 caaccttctc tgttaaccac aacaagagtg ctcgcgcttg gaaggcttga agagcaaaag
     6121 cgcttcgatc tgctcatcga tgcatttgtc cttatgcatg ccaagtatcc cgattggacc
     6181 ttgcgcatcc gcggtacagg ttccaaggag gctgagctca aaaatcgcgt caaagaactc
     6241 gggctcgaaa acgccatcga gatacttccc gccgtggaag atgttgcttc ggaatatcag
     6301 gttgcatcga tctatgctct tagctcgaaa tttgagggtt tccccatgac cctgatcgaa
     6361 gcaatggcat ttggtattcc ctgtgtaagc ttcgattgtc caaatggccc ttctgagatt
     6421 attgcgcaag gggaagatgg ctttctcgtt ccctatggcg atgtcacggc acttgcaggc
     6481 gcgctagcaa agcttgccca agaacccacg cttcgcatgc aaatgggccg caaggcaaga
     6541 gagcatatta agcgctttga ccaggaacat gtgagcgcac tatgggaaga ctttttagcg
     6601 aaactatgtc aagaataggt ctatgcccac cgtcagtgtt gtgattcctg tttacaatgc
     6661 catggctact ctcggcagag ctatcgaatc ggtgctttcc cagaatagta taggcaacac
     6721 gatagaaata cttattgtcg atgatggatc gaccgatcag accggaacaa ttgcaacaca
     6781 gtatgttcag gcatatcccg atcgcatacg acttattcat caacacaatc aagggcctgc
     6841 agccacaaga aataccggaa ttgcagcagc agtcggagag ctcattgcct ttcttgatgc
     6901 tgatgattgg tggcttcccg gcaagcttgc ttcacagctt cgtgtgttag aggagaagcc
     6961 ggaagtagcg ctcgtctgca ccaccatgaa tggacatagc ctctggggca aacctgctgt
     7021 catcatctta agatttaggg atctactctg gtctaataag gtctatacat cgagtgtgct
     7081 tgctaggaag acaattctcc tcgcagcggg ttgctttaat ccacaaagga gactttgcga
     7141 agattatgag ctctggcttt cgattggggc aacgcaccct atcgccttgc tcaatactcc
     7201 ttaccttatg tacagcaaag gcataggaac ctcatcacgc ctctgggcta tggaacgagc
     7261 tgaactcaag acctatgcta ttctaaagca aaggaagctt gtctctgggc caatctatac
     7321 aattttgcga tactggtccc tggcaaaata tatgattcga gttgtgcaaa agttcgcctt
     7381 aggaaagcac tttatatgaa agcaccttac ataaaaaaag cttctcttca gcgcatcaac
     7441 cttggtactg atccttgtaa ccctattgtc ctattgcacg ctgatgacta tgcttagtca
     7501 aaaaggctcg tgaaagatgg aaaagcacag cactatttat attgcagggc atcgagggct
     7561 tgtaggttcc gcgatcgtcc gatgccttac caaaaatggg tatgataacc tcatagtacg
     7621 cacccgccag gaactcgatc ttgctcgtca agctgatgtc gaagcatttt tccacacagt
     7681 aagaccaaaa tatgttttcc ttgctgcagc aaaagtaggc ggcataggtg caaattcaac
     7741 ctggcctgca gaattcatct accagaacct cgcaatcgcg agcaatgtca tcaatgctgc
     7801 ctatcgttca ggtgtaaaaa aacttctcaa tctaggatcc tcctgtatat accccaaact
     7861 agcaccacag cctctcaaag aagagtatct tcttactggg ccgctagagc ccacgaatga
     7921 ggcttatgct attgccaaaa tagcagcgat caagctctgc aaattctaca acgagcaata
     7981 tggcacaaac ttcatctcgg ccatgcccac caacctctat ggcgaaggcg atacctacga
     8041 cctcttttct tcccacgtgg tacccgctat gattcggaag tttcatgaag caaaaaagag
     8101 ccgtgaccct gtggtgctat ggggcgatgg gagtccctat cgggagtttc tctatgccga
     8161 tgattgtgcg gatgcgcttg tctttcttat ggagcagtgc gactatagca atataggaga
     8221 aatcatcaat gtcggatcag gtttcgaaga gacgattagg gaattggcta ccctcatagc
     8281 ttcggtagtg ggctacaaag gcaagatcga atgggatatc agtaagccta atggaacacc
     8341 ccgaaaactg ctcgactcgt ctaaattgtt ttcctatggc tggcgaccat ccgtggggct
     8401 tcgcgaagga atcgagcgta cctattcaga tttcttgaat agaaagaagg agatcattta
     8461 ggaatggcaa atggaaatga tgagacatgg gatgtcatca tcgaagcaaa aaagaacctt
     8521 ttgagttttg atataaaaga attattgaga tacaaagatc tcatcaagct ccttatccgg
     8581 cgggattttg tatcggtata caaacagact atctttggtc ccctgtggtt tctgtttcaa
     8641 cctctgatgt cgacgcttat ttacatgttc gtgtttggga acattgccca actaggtact
     8701 gatggtatac cccagccact tttctatttt tctggtacta tgctgtggac tttttttgcg
     8761 acaaacctgc agaaatcatc agataccttt gtgaacaata ccgggctttt tggaaaagtc
     8821 tactttccac gctttgcaat gcccatctct tatatggtca atgcgctttt cactttaggg
     8881 attcagtttg cgttcatgct gatcttttat atctattata tcgcgacagg ctatcacttc
     8941 gtgccgacag ggtggatttt ggcgaccccc ttgtacatca tccagctcgc tatgcttggc
     9001 atgggatttg gcctcttgac ttcagcgctc accactaagt accacgactt gcgcaatctc
     9061 gtgaactttg ggctctcgat cctcatgtat gcaacccctg tcgtctatcc tatctcgaac
     9121 gtacccgtaa aatggaagct gctatttcag ataaatcctg tcacaccagt tatagaaatg
     9181 ttccgttatt cctttcttgg caatggtaat cacgatcttc gtatgtggct ctatagcctc
     9241 gttgcaagcc tagttatctt ttggatagga ttagtcacct ttacttacaa cgaaaaaacc
     9301 tttatcgatg tgatttgaga aaccatgagc gtagcggtta gagttgagca tttatccaag
     9361 gaatatcgtc tcggtgtgat cggcaatggc agcctctaca aggatattca aagctgggtc
     9421 gcaaaaaagc gaggaaagcc cgatccttgg gtccccatcg atgagcgttc taaaatagga
     9481 cgtcaacatt tttgggcgct caaagacgtg agttttgaag ttgaacaagg agagcggctt
     9541 gggattatcg gtagaaacgg cgctggaaaa tcgacgctcc ttaaaattct ctcacgcatc
     9601 actgccccga cctcaggcgt tgtcaagatg aaaggccgcg tgggtagcct cctcgaagtg
     9661 ggaactggct ttcaccccga gctcacaggc cgcgaaaaca tctaccttaa tggcgctatc
     9721 cttggcatga aaaaagccga tatcgatcgc aatttcgatg cgatcgtcgc tttctcagag
     9781 atatcaggcg aattcatcga tacaccagtg aaacgttact cgagcggcat gttcgtgcgt
     9841 cttgcctttg ccgtcgctgc gtttctaaat tcagaaatac tcatcgctga cgaagtgctc
     9901 gccgtaggtg accaaaaatt ccaagagaaa tgcatcggaa aaatgcgcga cgtcagcgtc
     9961 aacgagggga gaaccgttct ctttgtgagc cacaacctcg gtgctatccg cgcactctgc
    10021 cagagcggga tcctgctgga gcatggcatt cttaccatgg ccgggccggt ggaagaagtg
    10081 acgaaaagat atgaggagga gttggcgaat ggatgaaacg gaatatacca agatcatcct
    10141 ttcaatcata gttcctgtat ataattctgc caagtttatt cgagcgactc tatcaagcat
    10201 cataaaaatt attgatagac gatctgacat agaggtgatc gtacaagatt catgcagcac
    10261 agatggaaca agcaatataa tatctagttt ttctgagaaa tacccattaa ttcatcattt
    10321 tattgaaaag gataatgggc agactgatgg aatcaataag ggtgcattga aagcaaaggg
    10381 attatggata acctggttgt gtggtgatga cttattgatg aacaccttta ggaaatcaat
    10441 agaaattctt cctcaagttg aagcagatgt tgtatatgca gattgtgttt ttttgttaga
    10501 aaatggtaat gctattccag caatcggtac agaagcctat aacaatggaa ttctagctaa
    10561 gagacgattg ataatgcaac aacctggaac ctgcatccga aaaagattat gggatattat
    10621 tggaggagta aataataatc tgaattgggt aatggattat gatctttttc taagattaga
    10681 aactaaaggt gccagatttc tgagaattga agaatttgtc gctatttcgc gtattcattc
    10741 tgaagcaaag acaagtagta gatcaataga gcgattattt gaacattgga gaatattgtt
    10801 taatgcacat atccataaaa taatctattt ttctttgaga ccttatcttt tatattttgt
    10861 ggaatatata ataaaaaata gagaatctca gtcaaaattt aaaggtataa cttatttgca
    10921 taaattattt tggttagttg gttatccagc tgaaaaagaa ttaattgaat ctagatttga
    10981 aaagaaaaag acagaaatcg aagcagaaat aagagttctt gacaaaggaa tcgctatatg
    11041 agtcaaggaa gcctctatgt gatcattcca actacccgcg gtgagaaagc gtttccatca
    11101 atctattctg cattgagtca aagaggattt gaaaatgtat ttgtcctaat ctgtgggaag
    11161 agcgcaaaca atcaagaatt tgcaaatcaa tgtttgaata agtttaattc aagagttata
    11221 gttcttcatt gtcctgctaa agagcatatc ttgcctggtg aagcccgtaa tattggaatt
    11281 gaatatgttg ttagatatgc aaacccatct gattatatat tatttttaga tgatgatatt
    11341 tgcattcctc ctaattacgc aatagactta aaaaatttta tcagttgtca tggattagca
    11401 gctgcaatgg gaaatatgac atcttttccc ataaatatat ggtcaaaaat aattgattat
    11461 tctaattttt ggtggctaca atgtaataga gactgtctta acagaggatg gctcggtgca
    11521 ggagctacta tccttaagat ttcagatatt tcgtcaattc gctatctatc ggatattgct
    11581 ataaatgaag acattgattt ttttgataga atttctacac taacaaccaa aaaattggga
    11641 atttgttctt ctattagaag cagacacttc cacaatagaa gctctttaat agggttgatt
    11701 aaatatcaat ttctcaatgg ctatagaggt cctatttacc atttaacccg caaacgagta
    11761 atactacaaa gtcttaagag gtgggttagc ttttctaaag ttgcaataaa agaaaacatt
    11821 aattatttga aatatcatat tgttgagttg attggcgtaa tatttagttt tcttatcgtt
    11881 gaatgtggag ctgtattgtc tactttgagg agaagataaa taatgaaggg caaaatagtc
    11941 attattgggc attcatatca tcttaaaacg aagtcaaatg aattttttct agatttactc
    12001 aataaacatt atgacgttga atttattgca gatgagtctt gggagaaaaa tgttagtcct
    12061 acgttggaat atatcgatga aacataccgc tctgttatat tttggcaaat gatttcccta
    12121 aattgtctaa aaaggataag gtgcaaaaat ataatatttg ttcctatgtt tgatcagagt
    12181 ggtggtgcac cgaaatcatt ttggtatcat ttaagaaatg taaagataat aagcttttgc
    12241 aaaactttgg gagacatcct tacaggcctc ggttttaatg ttctgaatgt aacctatttc
    12301 cctgagccag aggaaattcc gataaatgtt gaaccaggaa catgtttttt ttggcaaaga
    12361 acaaatatga ttgattggcc aaaggtcaaa agattactcg aaaaatcaga tgtcaagaag
    12421 gtccatatcc atactgctgt agatccaggt catagatttg ttaagccatc aaaagctgat
    12481 gagaaaagct accatattac ctatagttca tggtttaaga ctcgaaatga gtatttaaaa
    12541 ctagtggata aaagccaaat atacattgca ccaagatatc aagagggaat tggtctttca
    12601 tttttagagg caatggcaag aggaaaagta attattgcag cgaatcaacc gacaatgaat
    12661 gagtatatta ataatggctt taatggttat ttgttcgatc caaatgataa taagccaata
    12721 atttttgata atatagatca agtgaagcag aattctatcc aaactataaa agtagggtat
    12781 gaaaaatgga tcgatgatca agatagaata atagatttta ttgaggcacc aagcagtaga
    12841 aatttctatt ttagaaagca tcctttttct agatatgatt acgaggcaat gggcaacgca
    12901 atcaaaattg tgattaaata tatccttcct tttggaatcg tcaaagtaat atggtatttt
    12961 aagaaggata aaagatggaa aaagattctt aaggaagcca taaaaatatt cattccttat
    13021 ggaataatta agttagttag ctattttaga gctaaaaaga attgatacaa aataagagta
    13081 gaaatggatg gataaggttt tattttcgat tatcacagca acctataatg tcaaagggca
    13141 agatattgaa gcgaccaaag aatccttgtt aactcaagga acgaataatt ttgagtggat
    13201 tattattgac ggttcaacaa atgagcagag tttgagtcaa atttgtcatt ctatacaagg
    13261 tattcctatc aggaccactt tgcttgcgga gcgagatgaa ggaatatttg atgcatttaa
    13321 taagggaatt aaacttgcaa aaggggaatg gattgcattt ctaggttgcg gtgatattta
    13381 ttatgaaaat atattgatgc gcatagaaga taaacttaga actatatatg attatgacat
    13441 cctttatggc attactgata tcattagaga aaacggtgca cattattggt tttcaaggag
    13501 cgaggaatat cttatagaag gagaaatgat gcatcattcg gcttgttttg tgagaagatc
    13561 cgcctatgaa cagataggcg gatttgattg tagttatata tcagcttctg atttagatgc
    13621 attgatacga ttgtatcaaa gggggaaaaa attctatttc cttaacatga taatcacagc
    13681 gttccatgtt ggaggaatat caacaaaagg aagattgccc tatgaagaaa aaatgaagat
    13741 aatgaaaaaa tatggctata ttagcaagaa ggaacaagta aaatatgtta tcaaagcaat
    13801 aataaataag gccaaaaatg tgttctgcta ataggattat cgatgtatac aatcaagata
    13861 tattgcccta atataatttc tggttatggt ggtgccgagg gctatgtgct cacccttgcc
    13921 tcatacctca gcaataccta taagaatatc gatcttagca tggttatcta tgcaactaca
    13981 gaatgcaagg aatgcgcaaa tcatgaaaat gatcggccga tgaaaaatca gatgcgagaa
    14041 gagctcagta aaaaatataa tcttgcgttt tctgatgaat tagattttat tattctacct
    14101 gaaagcaaga ataatagcgt aatcagcaaa tattgtgcac atactaggct gcgggggaca
    14161 tctactggca ctgacctcta tataaatgga tttcataatg tacactattt tcatgggaga
    14221 aaaaatattc atattatcca tttccctgcg ccaccgcgtg ttcaggcatc accatttttt
    14281 acaaaacata ggtttttcaa gcccgttgct gcagcattag acagccggta tcgaaactgc
    14341 tatgaccttt ttctatgcaa ttctgcattc accaaaaaat ggctagaaca ttattggcat
    14401 gtcaatgaag atctcataca aatcctttat cctcctgttc tgattttgga tgaaaagcgt
    14461 gatagccaaa accgagatag cataaaccaa aaagaaaaaa atatactaat ggtatctaga
    14521 tttgatcctc gaaaaaaaat attagaacta gtcgatttct tcatcgaaaa caatgcgcac
    14581 tatccagaat ggagattact agtcgctggt tctacagatc ggcggtatca atcatatctt
    14641 gatgtgatag agaagagagc ccaaaataac aatatttata tctataaaga tatagcacct
    14701 attgagcttg catgcttata tcaaaaatcc tcaattttct ggcatgcaat gggcctcgat
    14761 gtggatgaag aaaaggaccc ccttgaccta gagcattttg gcattacaac ggtggaagca
    14821 atgtctgcag gagtggttcc tattgtcata aataagggcg gacaaaaaga aatcgtcgat
    14881 catggtatta atgggtatcg atggaacaat ttgaaagaac ttagaatgta tacgcgcgca
    14941 gtgatgctgg atgaaacgct tcgacaatcg ctcgcgcgtg cagctattga aaagagcaag
    15001 atcttttcaa gagaaagttt ttatcagcat gctgatgaga tatttcaacg cttttctctc
    15061 attccggagg agtaccgaaa ataatgttag cataccagag agattttgat gaaaaatttc
    15121 ctgacgtgag gcctgcaaaa acttctaata ttcgtcaagg acaaatggtg atgttgagac
    15181 tcctcaaaat attccacgca gcctgccaag aattggggct tacctattgg ctctgtgggg
    15241 gctcgctcat aggagctata cgacatggag gatttatacc ttgggatgat gatgtcgatg
    15301 tttttatgcc aagagagcac tatcaagagt tcaaacagca ggcaagcgaa gtgctgccgt
    15361 atgatgttta tttgcagaca gatacttcat ggatgcgtct tgtcgatcgc tttggttcaa
    15421 aagagataga agaaggaaat gatttcatct tcatcgatat tttccctgca aaacgctttc
    15481 ctcttggaag aaagatgctg cgaaaagtga ggatggtgat acctccctat cctttgccag
    15541 gaattcccaa agatgcatcc tttgctcgcc aattgaaaag gagagctatt cattatggag
    15601 caattttttt gagagtaacg ggcctctcgt atgtgataag gttcctctgt acccttggcc
    15661 cacggcgcta ttggtcctac gatctcgagg tgacctggcg tttttattat agagatgatt
    15721 ggatttttcc tttacactta catacatttg aagataccgc cttttttgta ccttgcgatc
    15781 cccataaagt actccagcat caatttgggg actatatgac gccgcctcca gagaatcaac
    15841 gcatcaatca tggtcataaa gcatttcatg tgacacaatc aaatggtcat cctgaatctt
    15901 tagagtggct tcagtttgca gagaagaaga aacaagctgc tgaaaaagct ggatggttag
    15961 actcatgcac accttcgtca taagcctgcc tgaggacatc gcccgcagag cctcaatcga
    16021 aacgcagctt gagcagcttg gtatagagtt cgaattcttc gatgcagtcc gcggttcaga
    16081 aaaaataagc gatccacgat ggtacgacga tgcggctgct cgaaagctcg aaggccgaag
    16141 tctaaagcca ggggaagtcg gctgcgcgct cagccatgct gcggtctaca cagaaattgc
    16201 gcagaggggc ctaccctggg cactcatcct cgaggatgat gcggcccttg ctcccgattt
    16261 accgcaggtt ttgagccttc ttgagcggaa ataccttgag caaggagacc ttgttgtcct
    16321 cagccgctgc gacttctact tgccgtggac aagaaaaaaa gtcaccgatc gctaccaaat
    16381 ggtcacgccg atatttatca aagaagggtc catcgctcaa actgtgggtt acatcatcac
    16441 ccaagaggct gctgtccaga tccgaaacat caacattcct gtaaaattcc ccgctgatag
    16501 ctggggatac tatcgccatc atgtacgttt caaaggcatt atcccgtcgc tctccctagt
    16561 tacacagcat gtcgaacttg ggagcactat aacaggcaat ggccagcgca catcttttca
    16621 gcgctattca ttgcagggta ttctttggaa tagttttaag acgtataatc cactcggcag
    16681 aaaactcaag tattatgcaa agcaatggca aaatagacat gggaagtccc caacaggcat
    16741 ttgaactttt gcttttagct atcgaacgcg acctccctgt actcagtgtt ggattgccct
    16801 ttatcataca ttttctaatg ccacaaaaaa taaccatcat tggctcatgt cactgccttg
    16861 aaaaagcacg gagcattggc cttgatgaat cctttgataa atcctcggat aaatcttccg
    16921 gagccattat cgaatacctc gacgaagatg ccgtagtgca aggcctgagc cttgcggcgg
    16981 taaaagcctg tattcaagct catgggggca aaggcgagcg agcagggtgg tattttaagc
    17041 agctattgct ccttgcatat gccacgcggc agagcccgta taactattac ctagtctggg
    17101 ataccgacac cattcctgtg cgtccaatga ccttttttga tgcagcaggc cgcattctta
    17161 tgacaacaaa aacagaggta catcctgcat atttcaggac tatggaaaaa ctgatcggaa
    17221 tcgggaaagt cgtgagcggt tcctttatcg cggaacacat gatgttcgaa cggccttatg
    17281 tttctcgatt attagtgtgc atcacgaaga ataaggcaag ggatgaatct ttcgatgccc
    17341 aaggatttat cgaatcggtc attgccgcca tcgagcccga agatcttgat ggttctggct
    17401 tttccgaata cgaaacctat ggttcattca tgtatcgcac tgcaggggag cgcatcgctt
    17461 tgagagatct tccttcaatg cgcctcggat catctttttt tgaccgacct ccaaccacta
    17521 agcagctttt tgcgctcagt aggcactatt attgggcgag cttcgagacc tggcatggta
    17581 ctacgttaaa aacgcgcata gcataccaag tgcgcaaagc aataggcgca ctgtggacca
    17641 ctggttcggt actcctcgca ccaaaggcct ttgatcgctt tcaaaaggag atagcgcatg
    17701 aataattcgt tgcgctatct ttcatttccg ccagacaatc ggcttagcgc acctgacccg
    17761 cttcgaggtt gccagcttgt catgttgcga cttttgagga ttttcgatgc gatctgtaaa
    17821 gagcttggtc tttgctattg gctcgattca ggcaccttgc ttggtgcagt gcgacataga
    17881 ggatttattc cttgggatga tgatctcgat gtcgcgatgg ttgatcatga ttatcaggag
    17941 tttctccata aggcaccatt ctaccttcct gcggatgtct ttctccagca tagagaaagc
    18001 gatcctcact acgatctacc ttttgcaaaa cttgttgatc gctatagcct gctcgattcc
    18061 cccgatggta aaaaggatgg agcactgagt ggcattttca ttgatattca gcccatgagc
    18121 tttttcactt atcgacaaaa ggcagcacga aaggtgctct atgttctgca tggcgactat
    18181 ggcaagccag acgcctcgcg aggagtacag agaaatgtga agcgcgctat tctcgcaacc
    18241 tttcgaacca ttgcactgat cacatcgctc gatcatgcgc tcgatagctt ttgcgcatat
    18301 ggagcaccaa aatggcttgc ctacgacttg tcctttcagc gctggtggcc tatttttcat
    18361 tcaatagatt cagtatttcc tttacagaca atacaattcg agggatatac cttctctgta
    18421 cctcacgaga tcgacgtgta cctccgcgct cagtttggcg actatatgac cttacctccg
    18481 cttgaggagc gcaggccggt acactcaggc ggcatccatc ttacaggtcc aaatccccat
    18541 cctctaggac tcaactggaa ggaccaccca tgaaaatcgt caaagtcata ggtggcctcg
    18601 gaaaccagct ctttcagtat gccttttccc gcgctctttc ccttaaaacg ggcgacgaag
    18661 tcttgctcga tatatcaagc tatacagaca cacagcgcca gatccattac ggttttgaac
    18721 ttcctcagct ttttcctatt cgctacaagg ttgcagatga gcttgacata aaacagctca
    18781 gcacgcaacc tcgctctccg ctttcgcagc tgcgtcgcag atatttcaga aagcgtactc
    18841 attacattga caaatacttc tgcttcaatc cagaagtctt cgagctcaaa ggcgattggt
    18901 acctcgaagg ctggtggcaa gatgaacgct actttgcatg gtgcagcgac attctccgta
    18961 aggagctcag ctttatcgct gatccaggtg ctcgcaatcg agatcttttg gctgaaatcc
    19021 aggctagtca tcatggttct atccctgtct cgctccacgt gcggcgtggc gatgcgctta
    19081 aaaaccctga tactcgggtc agtaccaccg cctactataa ccatgctatc gaagcggtgc
    19141 ggagccttgt tgctcatccg tactttctca tattctccga cgatcttcta tggtgcacag
    19201 gcaatctcgc gctcgatcca agcgaagcta tctatatcga ctggaaccga ggatgtgaca
    19261 gttggcgtga tatgtggcta atgacaaagt gtcgttgcca tatcattgct aacagcacct
    19321 tctcttggtg ggcagcatgg ctcgatgagc gtcccgataa aattgtctat gcccctgaac
    19381 gctggtcgct tgcacgtgca aggtgtttcg aatattacca ttataccttt gaccaagtgg
    19441 ttcctccttc ttggataaga ataccaattg aatgaatagg actttcgccg ctataatttt
    19501 acttaaatgt ttcccgactt tgatactctg attcctgatc tgagaccaga gggcgatacc
    19561 cccttgcgca gctgccagct cgtcatgctt cgccttctta aagtattcga ttatatctgt
    19621 aggaaagaag gccttagata ttggctggac gggggaacac ttcttggtgc tgtgcgccat
    19681 ggtggcttta tcccatggga tgacgatgtc gatgtcaaaa tgcctattga ggattatcgg
    19741 cgctttctga atcttgcgcc ccaagctctt ccctatgaca tatttcttca gacaaaaagt
    19801 accgatcctg ctcacactgc gccatgggca aaactgcgcg atcgatttag cttcatgaat
    19861 gaccctggag gtccctttgc ctattcgcaa ggcatcccca tcgatatctt cccggcagtc
    19921 tatatgacaa tgcgccagca caaatggcgc aaattcttct gcctcatgcc tccttatagc
    19981 atgccacccc aatggccagt gcgaacatgg tcacttcccc ataaactcta ttcaggcact
    20041 tttgcaatct tgcagactat tgcgaaacca atcctttcaa taaaggcttt ggctcgtgcc
    20101 ctccagcatt ggagtgataa aggagaaaag gtctggacca acgattatcc tatcgaatgg
    20161 atgcgcgaag catttcccga tgaagtggtc ttcccccttt ctcagattcg atttgaagat
    20221 gccatgtttc tctgccctgc tgatccagat cgttatctca ggattttcta tggagacgac
    20281 tacatgaccc cgccacctga aaataagcgc ggcgctcata gagtcgatgg ctttttgata
    20341 actggtccca atccacactt ctcaggtcta tgctgggaag actacgaaga aaagaagcgg
    20401 cgcgtcgccg caaaactacc atgagaatcc tccatatcgt caccaactcc gaccttggtg
    20461 gtgcgccgcg cgtggtcacc gaactctcag cacgggcagc taaagacggc catacctgtg
    20521 cagttgctgc ggcgccggat ggccctatgt ggcggtatct cgatgtacat gtacagcgct
    20581 ttcctgtgta ttcaatacgt agggaaattg accccctgga agatctcaag actttttgct
    20641 ctcttcagaa gctgtatggg agtttcagac ccgatattat tcaccttcac tcttcaaaag
    20701 caggtgtgct tggaaggcta tctgcgcttt catggggtat aaaatatact cggcgctgtg
    20761 tctataccat ccatggtttc gatacaattc ttaaggccca taataggtac ctcccactcg
    20821 agcgcatgct cgcgcgagcg acaggtgctg ttgtccccgt ttctcactat gatgagcgaa
    20881 atttgcgcgc tgccggaata tgtaagagag tcgtaatgat ccccaatggt gtcaccaatc
    20941 gaaggggaat gccaagcccc gacctacgcg ctgcttcatg cattgctgaa gcaaaagcac
    21001 agggaaagcc gatcgtgcta accatagcgc gcttagaaag ccctaagcgc cctgatcttt
    21061 ttgtcgacat tgcgagaaag atcacaaagg cttcgttttt ttgggtaggt aatgtgcaaa
    21121 atccaacttc tatgttacct gctggaacca aaattcctga aaatcttttt tttcttggtg
    21181 aatcgccaga agccggcaca ctcgcgaatt tatgcgatat ctttatgctc ctttcggact
    21241 atgaaggagc gccaatgtcg attctcgaag ctatgtcgtg cgcaaagcct gtagtagcgt
    21301 ctaatgtcgg cggcattcct gagattcttg gtgaaggctc agatgccgca ggtatacttg
    21361 taccaaacaa gccaaatatt atttcgaacg cagttttgag tattattgaa aaaccgtcga
    21421 tagctaatag acttggagct atggcccgtc agaagttcga atcatcatat tctgccgatc
    21481 atatgtggga gtcttatcta gatctttaca agaaacttag caaactatag aatgattttt
    21541 tggttttcgt aagtatatag tagcccctta ttaattgaag gccatgcaca aaagctatag
    21601 acatctgcag ttgagcatat ttttcgttga cgtattgcta atgtattgtg ctctcattct
    21661 cgctttgttg ctgcgtaata agagctttcc tgcaccttgg tctttgagga ttcacctttt
    21721 tcatttttca atcatatttg tcggatgggt gattgttttc tatacattgg gaatgtatcg
    21781 tctggagcgg cctttcgatg atctagtatt tcttcaacgc gtccttttag gctcccttat
    21841 atctggctta gcttctgcag gctatttcta ccttgcgccg agcgtgccta ttgagcctaa
    21901 gactgtactc ttcctctttg ttgtactcta cactgtcatg ttttggtgtt ggcggtacgg
    21961 cctcgcaaag cttagaagac ttcagagtct acgtgtgggt gtaggcttta tcgagcttca
    22021 gcctacttct tttgagatta tctatgagac tctagctcga agccctttgg gatacgatat
    22081 tcgttttgtt tataatccgc caccagatat tcagttgcct gagtcgattc gacgtgtatt
    22141 gcacgaggaa gatcttagag tggtgataga ggaaactgat actgatctca taattcttgg
    22201 tgagaataga ggaccttccg gtgagatgct cagggccctt tttgctcttc ttgatcgccg
    22261 ggttcgtttt atgcgcttat cagctttcta tgagctcgtg gtgaggcgtg tgcccgttgg
    22321 tctcatcgat gaaagctggt ttcttgagaa tgtagatctt aaagcaaaga agccatacga
    22381 aatagtgaag cgaggtatcg atatcatcat ggcagttttc ttgctcgttg tgacttctcc
    22441 cttttggcta cttattgctc ttgccataaa agctactagc aaaggcccag ttttctttcg
    22501 gcagacacgc cttgggcgat ttgaactacc ttttacaatc ctcaagtttc gcaccatgcg
    22561 gactgatggt aatggacatg aacctacgca ggttgatgat cctaggataa ctcctgtggg
    22621 ttcttttctt cgatcaaccc gcctagatga gcttcctcaa ctcctaaata tacttaaagg
    22681 agatatgagc tttattggtc ctcgcccaga gcgccccgat atagcataca cattaggaaa
    22741 agaaattcct tattaccgtc agcgccatct tgtaaagcca ggggtaactg gctgggatca
    22801 agtctcaggt caataccact catgttccgt cgaagatacc tataaaaagc tccaatatga
    22861 cctttattat ttgaagaata tgtcactata tcttgatgtt tcgattttct tcaaaaccat
    22921 catcacagtg ctcaagcgcg aagggagata aagttgcgaa tatatcgata tagatatttg
    22981 cttgataggg tggtgctggt aagcattttc tttgcttgtt tagtaggtcc tatgtcagct
    23041 ctggatttta ccgttggagc atctggagct tattccactg aaagtaggaa aaatttcttt
    23101 gttgatggat ccatcgccct gcatcatagc gaaggtccct ttacactttc atgggagctc
    23161 accgcagaca gcaaaggaac atatccggaa tcttttcttg ctgatttttt cggaccattt
    23221 tcgctcgata tcaagaatgc gggatttacc tatatggcca gacctttatc ctttttccta
    23281 ggtaaactcc cactaaagga tgaaatcaat agcccatatt ctttatttat ttctggcgct
    23341 tctccttcag tgatgactgg gggctttaga tttgattctg ggattttttc attttctgat
    23401 cagtggattg gacttgatca taatgtaaag aacaacctgt ataaaacgac agaagcaggt
    23461 atctatgctg atcggggtat agtgctcaag acatattcat tagatgttgg tgcactgaag
    23521 cttggttatc aagatgttgt actctttaca ggatcctact ttgatgtaga tatatttgct
    23581 attccagcgc cttctgcttt gctccaagat attctgacag cttcagggcg cccttcctcg
    23641 cgctcaggcg atatgaacag tattatgggt ttttttggcg aataccgcaa tgggggccta
    23701 aaactctgtg ctcagatact gatcgacgac ttcaatatga atagattcat tgctccttca
    23761 gcttatcaga atcctgacaa gatcgcatgg tcattaggat cagaaatcga tctttcgctt
    23821 ggtaaactaa gtctttatac agctggagca actaaataca cgtttgaatc ggtaagagaa
    23881 gaattttatt catatacgct tcatgcaggt tctgcaatat actcggatag ccagctcatc
    23941 gcggttcctc ttgaaaacca gatgcttggt tatatcaatg gtgaaaacaa tttggctttc
    24001 atggcgaatt ggagtgctcc tattgcgcag ctagacctgg aaacaggact tgaatgtgtg
    24061 atttcaggtt caaaggctcc gaccaatcca tggcataatg gtgaagactg ggcttcgctt
    24121 ggaacacagc tcctcaacga tcctgcgctc gaggtaaaat tgctgctcaa tctacgcatc
    24181 tcacgaaaat ggggggattt tactctcttt atgcaaacaa cgggtggcta tgtctttaat
    24241 caactcaatc cagtctatcc cggagaatca ggatacccga tcctgactta tagcgacggt
    24301 gatatgttgg agcctgtctt tgtaccaaaa gtgggtgatt ccgcgcctat tgcggagatc
    24361 atcctgggag gcatctggag tctttactaa tgattatttg tagggggaac ccttcatgcg
    24421 catagctttc tttgccgatg catatcgccc ccgcgtaagt ggccaggtag cgtctttaga
    24481 cgagttttgt agaagcctca ctgagcgagg gcatgaagtc tgtattgtct gtcctgagta
    24541 tccgcctgag cgcatgaaag gtcaatctga gcctttcaag accattcgcg ttccttcggg
    24601 ttgggcaatc gtatctgcgg acgatcggct tggtcttccc tggaaagggc agagagccat
    24661 gcgcgaattg gacgaatttg accctgaggc tgtgcatata cagacggagt tctccattgg
    24721 agccatgggg cgacgttatt gcagaaagcg cggtcttccc atcatgtcca cctgtcacac
    24781 tttctatgag atgtatgtaa aatggtacct gccttttctt ccggattttg tcgctaggcc
    24841 tgttgttaag acctggatga ggtcggtata tgcgcatgac gagcttatcg ttatcccttc
    24901 gcgtcgtatt cgcgatatca tgttgagcta tggcatcgac aaagaatacg cggtcattcc
    24961 gacaggcgtc gatgaacgaa tattctattc gcaaacagaa aaaggcaaag cttaccgtca
    25021 aaagctagat gaactttggc ctggtgtcgc tgagggctcg cttcttgtct atgtgggaag
    25081 gataagcctg gaaaagaatc tggagatgct cgcggatgca attaggcgcg ttttcgcaga
    25141 agatcctaat gcctaccttg ccatggtagg agatgggcct cgaaaagcag agattcaaca
    25201 gatgtttctt tgcaagggtc taaaagacag ggtcatctgg accggtttcg tgccgcgcga
    25261 agaattgcct attgtctatt ctgctgcgga tctctttgtc tttccttctg taacggaaac
    25321 tcaggggctt gtcactatcg aagcgatgct ctgcgggact ccggtcgtag gggtcaataa
    25381 gatgggtacc gctgaaataa tggaaggtga cacaggcggc cttttagctg aggatgatgc
    25441 tcgcgatttt gcgcggcaaa ttcttaggct tcttggtgat ccctctcttc gcgctgaaaa
    25501 agcctcccag gcgcttaagc atggccagaa ctggacaata ggccatctat gtgatgaaat
    25561 ggaaaagctc tacttgcgca tttttggatc ctaatgggga taagcgcact ttggagaggg
    25621 gtggcaagat tcttgcggcg atgaggtttt tattgatgag gcttttcatc atgaggttcc
    25681 ttttcaagaa aacatttgag gttcaattgc atttcgctat ggttttctgc gctgtgctca
    25741 ttctctgcgc tatgcagaac aaggcatatg cgcaggaggt aaccttatgg aatcctacaa
    25801 cagtagaaac gagctcgcag gggagtggag aagcgcagaa gtccattcat catccagctg
    25861 atcttttctt tcctggcgac gaagcctacg ataccgcagt ccttgaggca attcgtgcgg
    25921 gccatgtgcc cgagagcaat gtttttcccc tttttggcag tgagtttgac gtggtctcag
    25981 ctcccaagaa agctcccgga ggtaattctg aaatcaattc ttcaagcaat tctgagagta
    26041 gcctcgggag cacatgcgca gctccagttt ggcgttcggg ctttagcctc agcctaagca
    26101 cgcccaccct ctattatgat tcgcccgcca cgcaggacga tcttgcgtcc aatcttctgg
    26161 gcgagccaag cctcacagtc aatgcttttc cggagattca gcatttttgg aataccctgc
    26221 ccgatgtgct cgcacttgat atccgtctct atccttctcg atctatcgcg ttcaagatcg
    26281 atgcatcact cttttatcga cgcgataaaa ttatagacca aaactccacc ttccaatgga
    26341 acctcgatga atggctcggc aatctcttgg gagttgctga atttccccgc aaatcctatg
    26401 tggcctgggt aggcaaaggc tatggtgttg cgctaggcag atttccatct ggtatgggct
    26461 ggggaaagct ctctggttcc attctgaatc cgagagcctc gtggtacgat cagattcgct
    26521 ttcatctcga ttcaggtcca tttcgcttta ctagcatgat cgcgacgagc tcagcccaac
    26581 tctctaaagc tgaagaagaa atccagttca gaaaaaaaga ggatgggagc agtttttggg
    26641 actctcagaa cgaccacgac tatgcgggcc acgaccaggc catcaagctg gccaattggc
    26701 atcagatcga atggaaaccc gcgccctggc ttgaaatagc gctcgccgaa atgagtatta
    26761 tgggcggcag gttgccttcg ctcagcttcg tccttccatc ggcgatatgg cacaatgtct
    26821 atgcagcagg atactctaac gtgggagtat cgctaagcgc agctgctgta cctcttccgg
    26881 gcctgttgat atcgggagag ctctttgtcg acgatgtgtt gagctacgat gaagctggca
    26941 ccacgaagcc ccaagctttg gcctggctcg gtactgctcg atggtccaat gccctgactg
    27001 acaaactctt cctggatagc gggctcgaat accagcacgt tgatcaatgg acctacaccc
    27061 gctggaatcc ctatctcgct atgtaccagc gccaagctct taccggcggt aggcgcagtc
    27121 tcgaccaatc ccttggcgcc ccttggggac cggattacga tagcattggg gcttatgcta
    27181 atttgaggtt taggagcgga gcctccctgc aattgagcta tgagtttatc cgaaaagggc
    27241 ccatttatca gggaatggcg agccaggtaa acgttaccgc ggacttcgat ggcaacggaa
    27301 aaatagacca gatgccacag acaatctggg tgcctgtcta ctacgactac gacaaatatg
    27361 caggcgatgg cgctctagaa gctattcttt caagacccga cgaatatcga catctggtaa
    27421 cccttacggg cgcgctgccc ctaggagagg cggtggactt taagctttcc gcgaccttcg
    27481 gattttacca gaattttggc cacatctcgg gtgctaccga cactgctttt ctcttgtatg
    27541 cggggattgt cgtgcgttta gcttctcgat aaacacagaa ccagataaag tacaaaattg
    27601 tcgtataagg ttgaggggat tgtgtcaagt gatgttcgca atttggtttc aaagcagtca
    27661 ttggagcttc ccttccctta tgctgcctga tataacaacg cttgctgctt tgcacgcgaa
    27721 gcctctgaaa tataacaccg tcctgtctgc caatcctctt cgtattccat gaggtacgaa
    27781 cataagagtc gcatatatga atcgacgctt ggaaatatcc acaccacctt tgagcgcctc
    27841 cgaatctctt tgttcagccg ttctagggta ttcgtccatg atatcttcct cgcatcaatt
    27901 tttggaaatg catagaattg caatgaatcc tctaagcctt cctccagtac actaattgct
    27961 tcaggataat gtggtacata ctcttcgata atctcatgcg cataccgaat ggcagagtct
    28021 ctatctggct gtaaccaaat tgctttaagc ttttggccga atgctgcctt atccttctgc
    28081 gatactcggg ctaggatatt gcgcataaag tggaccttac accgttgcta tcttgccaca
    28141 agaaattctt tctggatcgc agccctaagc ccatggtgcg catctgagac acacaaccaa
    28201 accttcttaa gccctcgcgc ctttagtgat gatatgagag cgctgcacct gaagcgctag
    28261 gtgaagtatg attacttcaa t
//
LOCUS       DAOMZL010000015        27962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_1359_length_27962_cov_15.481456, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000015 DAOMZL010000000
VERSION     DAOMZL010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 27962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27962
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1359_length_27962_cov_15.481456"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PLQ30_02405"
     CDS             <1..533
                     /locus_tag="PLQ30_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01324.1"
                     /translation="SYSIHVQGYYNGMGYADSSIMQSAAARKAITEAIEEDYSTAYRY
                     SDSATEAGMWYLAGSASISGRFGEGDNITRITGSVYALTNFSDRSLRFNPQLKVQVGD
                     EGGKVSFTISDLTSIGDVGSEYAGKGNMTTPALTVSLLNSEVSLQASVPISLNADYSV
                     KKVQTKFSVIWNAIEF"
     gene            603..1310
                     /locus_tag="PLQ30_02410"
     CDS             603..1310
                     /locus_tag="PLQ30_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW01325.1"
                     /translation="MHGRILIVEDTKELAELYKLYLQNEGFECKLAFDAESALPIAQR
                     EDWDLIVLDLNLPGMDGFQFLEQLRNTKSMPVMILTAREADEDVIFGLGVGADDFVTK
                     PCPPKVLAARIRAHMRRTAKLQMRQNGSIVRFGPYELDLEGMYLTKNSEPISLSAREF
                     NILKELVSNPGRPFTPEDLYARVWGQEYGDISAIGVYIQRLRRKLEDDPTNPYYIQTQ
                     YGMGYRFNPEAFVEKAK"
     gene            1307..2746
                     /locus_tag="PLQ30_02415"
     CDS             1307..2746
                     /locus_tag="PLQ30_02415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPW01326.1"
                     /translation="MKLRLRTQFIILTMAVTLFPLVFGLLFFTGQQTKRDPRVTTRFF
                     IDEIAKEWSLDNNISLEDIRSIGARVGLPIMDAALIEPDGSVRVSSFKDLPAGSKIDV
                     IDLARPPPYGKPRPELRFLPIDASLEKSPLLIFDIQPFLSREDIRNRNFMLLTSFTLG
                     AFIVAGFMSFLILRSTEKAINNLIEDTAIVAGGKLDHEVKGSGALELHSLADSINHMR
                     ITLRDMISRRMNMLIGVSHDLKTPIALIQGYTDALSDNMASDEATRIHYLEIIREKSA
                     QLEDLVSELIEFMKIDDLNLPQEVVDLWPLLMSLGKRFEEDAHLLGLELQYGFGHFGS
                     ALSKEEPNDLPGVRMNRPLFERAVENLISNAFRFTGAGGHVELALDMKNGQPVLIVAD
                     SGPGISKDDLPYIFDAFYRGTHSRKEAGHGFGLTIVKSVVDLHGWSIEARNKKSPAQS
                     QGENHAQEGGKDQGLVIEIVMKPAPAAHD"
     gene            complement(2782..4146)
                     /locus_tag="PLQ30_02420"
     CDS             complement(2782..4146)
                     /locus_tag="PLQ30_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HPW01327.1"
                     /translation="MSLERKERKLFLKDGTIIMNMMNIAEKRRESGIGRLELEQLVDR
                     TIKESGALDASKILIIPPDITRFHSRAGLITDLLVEQLGSRVAAVLPAIGTHAPMTEN
                     EIAQMYPLCPRTLFRVHDWRKDVVELGTIPEERVAEISGGKVRYYYPIQTNKILAFGD
                     IDLVFSIGQVVPHEVAGMANHAKNIFVGTGGKEAIDKSHYLGATCGMESIMGRIDTPV
                     RNLFDDALDLSKTRLPAIVWILTVIGTAGDGSLMQQGYFAGYDRKCFEEACALSAEIN
                     IALFDKPLRKAVVWLDPSEYRSTWLGNKSIYRLRMAMADDSELIILAPGLRSFGEDAQ
                     IDAVIRSFGYRPSAEIQGLVAREPALSQNLSAAAHLIHGSSEGRFTISYAPGPGMTKE
                     EIESVGYHYIDLEKAKSRYLPEGRLAGAGFYNTLDGEEYFFVPNPALGLWSTAERMAG
                     VKKP"
     gene            4175..5410
                     /locus_tag="PLQ30_02425"
     CDS             4175..5410
                     /locus_tag="PLQ30_02425"
                     /inference="COORDINATES: protein motif:HMM:NF027582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HPW01328.1"
                     /translation="MLQLSRFSIGTGDRFGREGEAQIGAFKDMRAKGGEADIVWNKSN
                     REHVLIGSTPADQEKAASESIKKTGWDGRWFVDADHITMKTVDWFLPYCNFFTIDVAE
                     SIGQNASLESRSCFLDRAAFLLEDSSIPVRVEHADLESVADKFLAAVEEAGKTYRHIA
                     EHKPEGSFVVELSMDETDKPQTAAQIAAILVAVDAEKIPISTLAPRFPGKFLKGVDYI
                     GDATEFFHAFEDEAKVLLWASEYLNLPKGLKLSVHSGSDKFKLYKGIHDITARLEVGV
                     HLKTAGTTWLEEIVGLVEAGGKGLSVAKSVYEQAYLRIEELTAPYANVVEIDAQMLPS
                     PKMVAGWDSDTFVAALRHEPNSSTMQPSMRQLMHVGFKIAAEMGREYLDALEECRESV
                     SRNVRYNLYARHLAPIIFG"
     gene            5376..6992
                     /locus_tag="PLQ30_02430"
     CDS             5376..6992
                     /locus_tag="PLQ30_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPW01329.1"
                     /translation="MHVISRRSSSADFPSKGRLGFHRPGIMGKILICFGIIIIFFLVL
                     ALVMQNESMNLSREYSKNLSNYLLVHRFRLNLNSFHSQADRYLREPASVEINSIYDGI
                     LLLNSQYVELLPIADISIYVEFEVRATGYGLDAYFPLVSRAVGLRAGNSPDYYQVFVN
                     ASRIHGYIDIYLNRMLSELMKNGEETYVSLSHKAEILNRTIFFSMIFASAIAIGVILF
                     VTQAITAPLRRLAKEAEKLAKGDLDAGIVEVHTGDEVETLTRSFATMAMSIREYVEGL
                     KEKSELERLLHEEELALVSMGKALREAQFLNLQEQMKPHFLFNALNTIARSALLENAP
                     RTESLAICLATLLRATIKDSGALSTLDEELVIAQAYLEFQQVRFGDRLQWNIDVPRSL
                     RKTRVPRLMIQPIVENSVRHALEPKVSGGFISIKAQKRKEGLVLWFLDNGIGMSKERL
                     ADVRAKIAASLQVQSFDQINLPQVAEIPFSEENDVNEEELLEGRSIGLANIATRFGIL
                     YGDSCRFEINSRKEKGTLVRITLPLGEKVG"
     gene            6989..8365
                     /locus_tag="PLQ30_02435"
     CDS             6989..8365
                     /locus_tag="PLQ30_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW01330.1"
                     /translation="MIRILVADDEELERRAMIRILNSIPSNEIPDVMEASNGIEALDI
                     LQSQKVDIVFLDIHMPGVDGLKVAEKLSFLSNPPVIIMVTAYDYFSYARQALRYGVIE
                     YLLKPASTAEVHNALFLALREIKARKAEEERQAEAKRVTAGLKDFLQSKVCASLSENI
                     MDTEIIRQLVKLETGSETWLCIALVAGMKKFSSDDVLSLASCEFQRTFPVLAEKFLLS
                     DIGLAKANPMLFISPRGLRMEAMADEQMPDPIINVLIIIPHEIQKEEGNKIFQELFLY
                     RYSEHFEDQSIGRFLCRCRDAGAKTLRIGISAADYRSASIALRMATTAFNLSSVERPI
                     IVLNPIQTDEPKAKDTHKIASIALLWIEDHFMENIGLKDLAKAINISPSHLSRLLMKE
                     TGISFGEILSRIRIARAKSLLLNGISVKVTSFLVGFRDQSYFTKVFTKIEGISPSRYI
                     EQFQSRSN"
     gene            8504..9481
                     /locus_tag="PLQ30_02440"
     CDS             8504..9481
                     /locus_tag="PLQ30_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPW01331.1"
                     /translation="MKKFGIIALALLLILLPSMLFAQAKPIVLRLAETHPKGYPTEMG
                     DEEFARLVKERSNGRIIVEVYPGSQLGEEKAVIEQVQFGAIDMTRVSISPVAAFVPKL
                     NAFQMPYLYRDSDHMWKVLKGNIGMELLASLEPFGFIGLGWFEAGARSFYNSKKPVYA
                     PSDLKGMKIRVQESELMLGLVRSFGAIPTPMPYGEVYSGLQTGVVDGAENNPPSYYSA
                     SHYEVAKYYTLDEHTMVPEIIIGSKISLGRLSKADQDLIKQAAMDAIDYQRAQWDAYV
                     KVSMDKVKAAGCTIIPIPDKTEWMKAVEPMYKQQSKEIQDLVARIRAVK"
     gene            9608..10147
                     /locus_tag="PLQ30_02445"
     CDS             9608..10147
                     /locus_tag="PLQ30_02445"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HPW01332.1"
                     /translation="MKKFIVSAVTLIHILLINLSMIMIVAMLLLVFVNVVLRYVFNSG
                     IYWSEEVALVLEVWFIFLSLGLGVKYRLHFSINLANREKISASLNRALDILTDIIYIF
                     IGAVMIIYGSKLVRFTMSSVMPATMLPAGILYLVLPLSGFVAIVEAVFHVFGFNMFDE
                     KLEAYFSGQGIRIRDVLRS"
     gene            10152..11453
                     /locus_tag="PLQ30_02450"
     CDS             10152..11453
                     /locus_tag="PLQ30_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPW01333.1"
                     /translation="MDVTIPTIILMGGFVLLLLLRVPITFSLAAASIATAIYLHIPLM
                     AIVQQMVQGVKSFSLLAIPFFIIAGEMMARGGIAEKIINLANVLVGRVRGGLGMVNVV
                     DSMLFGGISGSAVADVASTGVIIIPMMTKNGYDNDFSVAITVTSATQGIIIPPSHNMI
                     IYSVAAGGVSVGRLFLGGYIPGILLGISLAVIVYSIAVRRNYPKGRKYSFKEGIKIAI
                     DAFWGLLVAVIIIVGVVAGVFTATESAAVAAVYAFIITFFVYKQIPLREFRSILYSTL
                     KTLTMVMTLIATASSFSWLMAYLRIPAMATEILLSITDNKYLLLFLVNVLLLFLGMIM
                     DMAPLILIVTPILYPVLIGKFGMSPIHFGIMLMINLAIGLCTPPVGSALFVGCAIGKI
                     SIEKATKAMIPFYIAMIATLFLVTYVPEITMFLPNLIMPGQ"
     gene            11507..12334
                     /locus_tag="PLQ30_02455"
     CDS             11507..12334
                     /locus_tag="PLQ30_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW01334.1"
                     /translation="MSFIESMFSLEGKIAVVTGGGGMLPSYIAVAFAKAKAKVVLWGR
                     GTHHPMEDAARMVRKQADLPIDCSDVIGITVDTGSTDACEQALEQTIHSFGLPDILLN
                     GVGGNIGRSKFVELDEKIFEEVLKLNLMAGLVIPSKVFAKRWIAAGIKGCMINMTSMT
                     SYKPLSDTWAYDAAKAGVLNLTQGLAKEFAPYGIRVNAIAPGFFIGNQNRALLIDEET
                     GDLTPRGKAIIARTPFGRFGEYEELWGTALYLASSKASGFVTGVSIPIDGGFLIDNI"
     gene            12406..13845
                     /gene="uxaC"
                     /locus_tag="PLQ30_02460"
     CDS             12406..13845
                     /gene="uxaC"
                     /locus_tag="PLQ30_02460"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF002794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HPW01335.1"
                     /translation="MADFLDEDFLLKNETAKRLFFEIARDLPIFDYHCHLSPKAIAED
                     KPFDDLAKIWLEGDHYKWRIMRANGAPESLCAGAGAWDEKFLAYAGALTKAAGNPLVQ
                     WSHLELKRIFEIDEVLTPQSALSIREKANRILSEKSISPRSLLEHFKVSVLCTTDDPV
                     DTLAWHRAIAEEQLSGSSFKTKILPAFRPDKAMKTEDIETWNVYIDSLGEAAGIEIDS
                     FASLADALTSRHAYFHSMGSRLSDHALLVPPFVQAIDTELDSIVKKGRSRIALSQIER
                     AKLETALLLHFGRLNAKKGWTMQLHIGALRNINPGLNRVFGPDVGGDCTSDAPIIAPL
                     ASLLETLEREGNLPKTILYSLDPSKHNPLAVLAGSFAGTAPEISSPQVAGKVQLGAAW
                     WFNDQKDGMERHMKEYAGVGLLGAWVGMLTDSRSFMSFPRHEYFRRLLCNIIGEWVEA
                     GELPDNPLYTEQLVRGICWENAVNYFGML"
     gene            complement(13976..14851)
                     /gene="ugpE"
                     /locus_tag="PLQ30_02465"
     CDS             complement(13976..14851)
                     /gene="ugpE"
                     /locus_tag="PLQ30_02465"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HPW01336.1"
                     /translation="MNEQQLSNKAAHRAKVLRSLYPHIILWIGIVIIIFPIYFIFSAS
                     SHTNADIQAAPMPLGIGPHLIENYRQALLHGTQNMGTPVLTMLKNSLIMALGISIGKI
                     VISLMAAFAIVFFDFPLRGFCFWSIFITLMLPVEVRIMPTYKVVADLGLLNSYAGLIL
                     PMIVSATAVFLFRQFFMSVPREIAEASQIDGATPMQFFSHILAPMTRTPIAAMFVIQF
                     IYGWNQYLWPLLITTKPEFYTLLIGINRMMSGADVQIEWQIVMATTMLAMLPPVIVVV
                     LMQKQFIAGMTETEK"
     gene            complement(14868..15752)
                     /locus_tag="PLQ30_02470"
     CDS             complement(14868..15752)
                     /locus_tag="PLQ30_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPW01337.1"
                     /translation="MSKQYEFRASILPYLLVLPQMLIVFIFFFWPSAQAILQSFYLQD
                     PFGGKMIFVGFENYSNLLIDPDYWQSFWVSTVFALCITISSMAISLFLAIQANKKIRL
                     ASFYKTMLIWPYAVSTLVAGVIWLFMFNPNVGVIAYTLKHTFHIDWQYLLNFNQAFLL
                     LTMAASWKQLAYNFVFFLAGLQSVPATLVEAAEIDGAGPTKRFWKIIFPLLSPTTFYL
                     LVMDLVYGFFETFPIVHSITSGAPGKSTAILVYKVWRDGVINLDLGSSSAQSVILMIM
                     VIVLTVFQFRFIERRVTY"
     gene            complement(15927..17228)
                     /gene="ugpB"
                     /locus_tag="PLQ30_02475"
     CDS             complement(15927..17228)
                     /gene="ugpB"
                     /locus_tag="PLQ30_02475"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HPW01338.1"
                     /translation="MKRVVLLIAVLLMAVGAYAQTTVELWHAMAGHNGEMVQIISDQF
                     NASQKQFKVVPVYKGSYVDTMNAGIAAFRAGQAPAIIQVYEVGTATMMAAKNAIKPVY
                     QLMAENKINWSSDIYIPAIKSYYSTSDGRMISMPFNSSTAVMYYNKDAFRKAGLDPEK
                     PPVTWPEFFEVAKKLKASGMEGGFTTNWISWIQLENFCAWHNIPFGTRSNGFDGLDTQ
                     LVFNSPIVVRHFQNIYDLSKTGVFIYGGRENKANPLFTSGRVGLHFESIGGYGNMKAT
                     CKFDFGVARLPYYPDVPGVPQNSCIGGASLWVFNGRPAAEYKGVAEFFNFISKPEIQA
                     LWHKETGYLPVTQAAYELTKSQGYYVNNPGLEVAIKQLTNKTPTPNSMGIRFGNMPII
                     REIEDQVWEEILAGKISVKDGLDKMVREGNKQLRSFEKLNK"
     gene            complement(17351..18361)
                     /locus_tag="PLQ30_02480"
     CDS             complement(17351..18361)
                     /locus_tag="PLQ30_02480"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPW01339.1"
                     /translation="MGKLSMKLCNFPLSKFREIFIINKMNIKPCNRLRNKALRVFFLM
                     GAGLALFGQNIPKSLSMEKLIMQSYRPIVIAHRGFRGIAPENTLLAAQKGFDVGADMW
                     ELDVAASSDGILVVLHDNTLTRTTDAKTRFPSRSPWIIYDFTFDELKSLDAGSWYSKT
                     DPFMQIAYGRVGSSELESFKDLRIPTLREALELTKRLGWRVNIEIKDATGFACDAWIV
                     EKTAELIKELDMVPSVLISSFNHDYLKRMKMAIPEISVAALIDKPISDPVQRLKDIGA
                     VALNPNEKYLDEATVRALRSAGLGVLPWTVNDPSRMRMLLNWGATGLITDFPDRALEL
                     VK"
     gene            18399..18977
                     /locus_tag="PLQ30_02485"
     CDS             18399..18977
                     /locus_tag="PLQ30_02485"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPW01340.1"
                     /translation="MVAGDGNGGRKWMEISTRKVYECNVFSVLERESKGPDGRTGHFS
                     LMRAQDWAVIVPYVRTDAGTSFLMVRQYRHGAEELSLEFPGGVVENGEEPSHAAAREL
                     AEETGWVSDKILHAGTVFPNPAIQDNHFHVFVALDPKPAVDRHLDQNEIIDAYLVDAD
                     EVRRRIGEGELRHALMVTALYLADRLVGSLKA"
     gene            complement(18998..20602)
                     /locus_tag="PLQ30_02490"
     CDS             complement(18998..20602)
                     /locus_tag="PLQ30_02490"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPW01341.1"
                     /translation="MAHVSLDGLIRFGNLSDLETMDLIAPMEKNGYLVLQADALRECS
                     RIAFTKIAYLMPETQYDAFAKIIEDPEASEYDRFVASCLLENALISGKRRYPICQDTG
                     TALVYGWRGDRIEIEKNIDLEELLVQGAAQAWTQEKLRNSQMGPLNMMEECNTKDNLP
                     AGIQIRSIKGKELRLTFVAKGGGSASRSSISIESPAILRADKLENVLRLRINALGPSG
                     CPPYAIAMVLGGTNPAQSLYAAELAALGLLDNLPTSANGDGMPMRDILWEKKMLTIAR
                     DSGFGAQWGGSHFAVSTRAIRLSRHAANLPLAIAVSCAANRHVSVFIDNSGWYIEKLS
                     PPSQCLENKAQEIILQTSAKAEIIQITQSDDSWLSKLRSLQAGTMALISGPVLLARDM
                     AHARLLRMLESDKKLPDWFIGLPVFYAGPTEAKQGAKTGAFGPTSASRMDSYLEAFMK
                     VGASIVTIAKGSRSNEARRAIATYKGVYCAAIGGVAALNAERFLQKLDTIQFEDLGME
                     AVRIAVIDRLPVIIAIDSHGKDIYDS"
     gene            complement(20651..21817)
                     /locus_tag="PLQ30_02495"
     CDS             complement(20651..21817)
                     /locus_tag="PLQ30_02495"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPW01342.1"
                     /translation="MKPERADVALIRCESYEAEKLDKTIETCAEIASLPDLQGKTILI
                     KPNVLNASPASKAVTTNPAFVEAVIRFAKSRGAKEILVGDSPGWQPVMLAAKTSGIYD
                     AVQQSGGTWADFREVSPHAVKEGKKLKNIPLTSLVEEVDLVINLPKLKNHGLMTYTGA
                     IKNLFGLIPGTAKSAMHLQYPAVIDFGEMLVDLALSIPNCFTFMDAIIAMDGQGPGSG
                     TPYSLGIVLASRSVAKLDWIASQCVGYDPRNISYILDAMERTSGSANITEPSTYPLRV
                     SEIEHSGFRRLPYTSKLGKRLSAIPRGLRSLAGKLIHLRPIFHKNKCIGCSACIEICP
                     AKVLLLDRKNGSNIIRINDKLCITCFCCHEVCPAKAITVGRAPVRIVHRRLPGL"
     gene            22049..22888
                     /locus_tag="PLQ30_02500"
     CDS             22049..22888
                     /locus_tag="PLQ30_02500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPW01343.1"
                     /translation="MLLGSSFRPAPGPMTARGGSDVSDFAKSNEIAMPEPEDKLVKYS
                     VYEVKKGDTLSEIAEYFDLTLDTLISINNFKSAKSLKPGQLLKIPSESGILYIASKPT
                     TIQEIADSYNINIDRLIETNGLLSETIPAGKPLFLPDVKLPAATLREIAGTLFQWPVK
                     GRLTSWFGWRKDPFTGRRSFHNAIDIAAPYGSSITAPMDGRVIETGYSPILGKYAIIS
                     HSGGWKTLYGHMSEILVQEGQYVSQGRTIGRIGTTGYSTGPHVHFEVIKNGVLVNPLN
                     YLP"
     gene            22977..24698
                     /gene="ptsP"
                     /locus_tag="PLQ30_02505"
     CDS             22977..24698
                     /gene="ptsP"
                     /locus_tag="PLQ30_02505"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPW01344.1"
                     /translation="MMIIKGIGVSPGVAIAPAFVHIDDSGIIPQYHIAIDDVQSEMAR
                     FHEASLLAKKEIEALRELAKNEAGEEQAAIFDAHIMMLDDPEFLEQIERSLKEKQFNV
                     EVAILSFESEMVNRLSSSLDPLKQEKVSDIHDVVRRLLGHLLKKERISLSDIQTEIIL
                     VAKDLLPSEMVMMSRSMVRGIVTETGSRTSHAAILARAFEIPAVLGVGPGFILQVRQG
                     QSVYIDGDNGTVVIEPDDSIIQSEKSARAARLNQEKENRELRDVPAHTKDGTSIMLLA
                     NIGMPEEVVNAIEHGAEGIGLFRSEFFFLGGHIPGEEEQYNAYSQVARSMHCRPVVIR
                     TLDIGGDKVLPELGVIGEKNPLLGWRAIRFCLEKTDLFKTQLRAILRASGQGKVNIMF
                     PMISNIDELIRAQAVLAEAKEECKSKGYKIDEDIETGIMIEVPSAAICSDVLSRSCDF
                     FSIGTNDLSQYTLAVDRGNQKVAYLNNPFDIAVLRLISMTIKSSKKANIGVSLCGEMA
                     ADPASAVLLVGLGLRSLSMSASAIPAVKRALMSITMEEASSLAQQALSMNTASQVTAL
                     LNSRLNL"
     gene            24707..26152
                     /gene="der"
                     /locus_tag="PLQ30_02510"
     CDS             24707..26152
                     /gene="der"
                     /locus_tag="PLQ30_02510"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPW01345.1"
                     /translation="MSKKKQTVEPLEIDLQQKGALSQSKEKEESSPNIRYENLPLVVL
                     AGRPNVGKSTLFNRLIGKRKAITDAKPGVTRDPIEEECELLGTNKRICLVDTGGFKLE
                     REGLDELVVARALAYIQRADLIIFIVDITQITPEDEEFAAFLRPYAKKLLLVVNKADS
                     PERDALVWEHARWGFGPMLFISAEHNRNIDKLEEAITARLDFSSVRQAEVEAADIRIA
                     IMGKPNTGKSTLLNALVGYERSIVSPEPGTTRDIVKDHFQYEGRGFEVLDTAGIRRKK
                     KVTDNIEYYSVTRSIRIVSQADVVVLLIDAEEGLSDQDKKIAAFAVEAGRPVVFALSK
                     WDKMPNMKNAFEATRDRLRFFFGQMAYAPIVPISAKERKGLDKLMSVVISLYAKANKV
                     IETSHLNQAVAEWVKETPPPASPSNRFKLRYAVQSSVNPQRFIFFVTRPEAVPESYKS
                     FLKNKIRLDFGLDGVPIQLELRASRRNRQRS"
     gene            26156..26581
                     /locus_tag="PLQ30_02515"
     CDS             26156..26581
                     /locus_tag="PLQ30_02515"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPW01346.1"
                     /translation="MIYLSIGQLEKMLSVPASTLRFWEQEVPFLMPSRTKSGRRCYSL
                     SEVVLIARLKHLALDRGLGLSYTQRILEYELLYQDQELRANIKQLREELFRLLLECDR
                     WKVSFKGLLSVSALENSTKENSEKELLNGRSDKESDGFR"
     gene            26544..27869
                     /locus_tag="PLQ30_02520"
     CDS             26544..27869
                     /locus_tag="PLQ30_02520"
                     /inference="COORDINATES: protein motif:HMM:NF002759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HPW01347.1"
                     /translation="MEEAIKNLMDFVDASPTPFQAIDNLIGILEAHGAIKLDERELWQ
                     LEPGVSYYIVRGGSSLVTFRMGLKPPSECGFLLAGAHTDSPGLKIRPEKELQSRGYLR
                     LALESYGSPILSGWLDRPLGLAGALAIQENQEIHQRLYISHEPIAVIPNLAIHLNRDI
                     NKGFEYNLHQQLPALFGLTSHKQKEPEAASEFGKTKEISGAFDSEILVRISQELDVDP
                     KAIISSDLRLYDPEPTRIFDNSLINAPRLDDLAGCAALIEAFISLHAQASTQVACFFD
                     AEEIGSMTTGGAQSSYLRDILARITLASRSLGQDFYRALACSACVSIDGAQAWHPGYP
                     DKFDDFYTPLLGRGIALKSNANMNYATDFTSQQIGIRVASKADIKPQIYMARADIQPG
                     KTIGPITASRTGIPTVDVGHPLLAMHAIRETIAASDHLDMIAFLRAFYS"
BASE COUNT         7622 a         6154 c         6718 g         7468 t
ORIGIN      
        1 tatcgtattc gatacatgtt caaggttact ataatgggat gggttatgca gattcgagca
       61 taatgcagag tgcagcggcc agaaaagcaa taacagaagc aatcgaagaa gattattcta
      121 ccgcttatag gtatagcgat agtgcaacag aagcgggtat gtggtatctc gctggatccg
      181 cgtcgatatc gggacgcttc ggggagggtg acaatatcac ccgtataact ggctctgtct
      241 atgctcttac caatttttcc gatcgctcac tgcgctttaa tccccagctc aaggtacaag
      301 tgggagatga aggaggaaag gtgagcttta cgatttctga tttgacttct atcggcgatg
      361 ttggttctga gtatgcggga aaaggcaata tgacaacacc agctcttacc gtgagcctgc
      421 tcaacagcga ggtatcgctt caggcctctg tgcccataag cctaaatgcc gattattcgg
      481 tcaagaaggt tcagacgaag ttcagtgtta tctggaatgc aattgaattt tgatgctatg
      541 aaatggagaa taaatgcaag tagcctggca ctgttgccaa gcacgtttgc agaggagggg
      601 gcatgcatgg aagaattcta atcgtcgaag acacaaaaga gctcgctgag ctctataagc
      661 tctatcttca gaatgaaggc tttgagtgca agcttgcatt cgacgcagag agcgctctcc
      721 ccatcgcaca gagagaagat tgggatctca tagtgctcga ccttaatctt ccgggaatgg
      781 atggctttca attcctcgaa caactcagga atacaaagtc catgcctgta atgattctca
      841 ccgcaagaga ggctgatgaa gatgtaatct ttgggctagg tgtgggagcg gatgacttcg
      901 tgactaaacc ctgtcctccg aaggtccttg ccgctcgtat ccgagctcac atgaggcgca
      961 cggccaaact tcaaatgcgg caaaatggaa gcatcgtaag attcggacct tatgagctag
     1021 atctcgaggg gatgtattta accaagaatt ccgagcccat cagtctttcc gcgcgcgaat
     1081 tcaatattct aaaagaactt gtaagcaatc caggcaggcc atttactcca gaagacctat
     1141 atgcacgcgt atggggtcag gaatatggag atataagcgc tattggagtc tatattcaga
     1201 gattgcgcag gaagcttgag gacgatccga caaatccata ttatatccaa acccaatatg
     1261 gaatgggcta cagattcaat cccgaagcct ttgtggagaa agcaaaatga agcttaggct
     1321 ccgaactcag tttattattc ttaccatggc tgtaacgctt tttcctctag tattcggatt
     1381 actcttcttt acaggccaac agaccaagcg tgatcctcga gtaacgaccc gtttctttat
     1441 cgatgagatc gcaaaagagt ggagcttgga taacaatatc tcactagagg atattcgaag
     1501 cataggagcg cgagtaggat tacccataat ggatgccgcc ttgatagagc cagatggctc
     1561 tgttagggtt tcttctttta aggacttgcc cgcaggaagc aaaatcgatg tgatagatct
     1621 tgcgcgacca cccccttatg ggaagccgag acctgagctg cgctttttac ctatcgatgc
     1681 aagcctagaa aaaagccctc tcttgatttt tgacattcag ccttttttat cgcgagaaga
     1741 tattcggaat cgaaatttca tgctcttgac gagctttacg cttggagctt ttattgttgc
     1801 gggttttatg tccttcttga tactccgatc gacagaaaag gcaatcaaca atctaatcga
     1861 agatacggcc atagttgcag ggggtaagct cgatcatgag gtaaagggaa gcggcgcgct
     1921 agaattgcac tctcttgcag acagcatcaa ccacatgcgg atcacacttc gcgatatgat
     1981 atcgcggcgg atgaacatgc tcatcggcgt gagtcatgat ttaaagacgc ctattgctct
     2041 tatacaaggc tatactgacg cattgagcga taatatggcc tccgatgaag ccacaaggat
     2101 tcattatctg gagatcattc gagaaaagag tgcacagctt gaagatcttg tttcggaatt
     2161 gatcgaattc atgaagattg atgatcttaa tttgccacaa gaggttgtgg acttgtggcc
     2221 tcttcttatg tctctaggca agcgttttga agaagatgca catcttctgg ggctcgaact
     2281 gcaatatggt tttgggcatt ttggttccgc tttatcgaaa gaagagccca atgaccttcc
     2341 aggcgtaaga atgaatcgtc cgctattcga gcgcgctgtc gaaaacctca tatccaatgc
     2401 atttcgcttt acgggagccg gtggccatgt ggaacttgct ttggatatga agaatggcca
     2461 accagtattg atagtagctg atagcggacc tggaatttcg aaagatgatc tgccatacat
     2521 cttcgacgca ttctatcgag gaacacattc tagaaaagag gcgggtcatg gttttggtct
     2581 tacgattgta aaatccgttg tcgatcttca tggttggagc atagaggctc gcaataagaa
     2641 gagcccggct cagagccagg gcgaaaacca cgcgcaagaa gggggcaagg accagggctt
     2701 ggttatcgag attgtcatga agccagcacc ggcggcgcac gattagctct tgaagcttct
     2761 gatattcctt gagcaagagt atcaaggctt tttgacaccg gccatacgct ctgcggtaga
     2821 ccaaagacca agggctggat ttggaacaaa aaagtattcc tcgccatcca aagtgttgta
     2881 gaagcctgcg cccgcgagcc tgccctcggg taagtatctc gatttggctt tttcaagatc
     2941 gatgtaatga tagccgacag actctatttc ttctttagtc atgcctggcc caggggcgta
     3001 gcttatagtg aagcggcctt cgctggatcc atgaattaga tgggcagcgg ccgaaaggtt
     3061 ttgagagagg gcaggctcgc gggctacaag accttgaatc tcggctgaag gcctatagcc
     3121 aaagcttcgt attacggcat cgatttgggc atcttctccg aagctgcgaa gtccgggcgc
     3181 aagaattatg agctcgctgt catcggccat ggccatcctt aggcgataga tcgatttgtt
     3241 tccaagccat gtactacgat attcgctggg gtctagccag accaccgctt ttctaagcgg
     3301 tttatcgaaa agcgctatgt taatttccgc ggatagggca caggcttctt cgaagcattt
     3361 tctatcgtat ccagcaaaat atccttgctg cataagtgag ccatctcctg cggttcctat
     3421 aacagtgaga atccagacaa ttgcaggcaa tctagtctta gaaagatcta aagcgtcatc
     3481 gaagagattt ctaaccggtg tatctatacg ccccattatg ctctccattc cacaggtagc
     3541 gcctagatag tggctcttat cgattgcttc ctttcctccg gtacctacaa aaatgttctt
     3601 cgcatgattg gccattccag cgacctcatg aggtacaacc tgtcctatcg agaaaacgag
     3661 gtctatatcc ccaaaagcaa gaattttatt tgtctgaatg gggtagtaat atctgacctt
     3721 gccgccagaa atctctgcaa cgcgctcttc tggtatcgta ccgagctcga ctacatcctt
     3781 tcgccaatca tggactcgga atagcgttct tgggcaaaga gggtacatct gagcaatctc
     3841 attttctgtc atgggtgcgt gagtacctat ggctggcaag acagcagcca cccgagaacc
     3901 aagctgttcg accaataggt cggttatcaa gcccgcccga gagtgaaatc tcgtgatatc
     3961 agggggaatt atcaagattt tgcttgcatc gagcgcacct gattctttta tagttctatc
     4021 gactaattgt tcgagttcta atcggccaat gccagattct cgcctttttt cggctatatt
     4081 catcatgttc ataatgattg ttccgtcctt caaaaagagc ttgcgttcct ttctttcaag
     4141 cgacattata tagaaaacat ctcgggaggg acatatgctg caactttctc gcttttcgat
     4201 aggcacaggc gaccggtttg gtcgcgaagg agaggctcag ataggcgctt tcaaggacat
     4261 gcgagccaaa ggaggagaag ccgacattgt ttggaacaag agtaatcgcg aacatgtact
     4321 gataggctcg acgcctgccg atcaagagaa ggctgcatcg gaatcgatca aaaaaacagg
     4381 ttgggacgga aggtggttcg tagatgcgga tcatataaca atgaagactg tcgattggtt
     4441 tctgccatat tgcaattttt ttaccatcga tgtggcagag agcataggac agaatgcttc
     4501 attggaatcg cgctcatgtt ttttggatcg agcggctttt ctgctggaag atagttcaat
     4561 tcctgtccga gttgagcatg ctgatctaga gagcgtagcg gataaattct tagcggccgt
     4621 cgaagaagca ggaaaaactt accgacatat cgccgaacat aaacctgaag gttcttttgt
     4681 tgtggaacta tctatggacg aaactgataa accgcagact gcagcacaaa ttgccgcaat
     4741 tcttgttgca gtggatgccg aaaaaatacc gatctcgact cttgctcctc gctttccggg
     4801 caaattccta aagggcgtcg attatatcgg agatgcgaca gaattcttcc atgctttcga
     4861 agacgaagct aaagtccttt tatgggcttc cgaatatctt aatcttccga aaggcctaaa
     4921 gctgtcagtt cattctggca gtgataagtt caaactttac aagggaatcc atgatataac
     4981 tgcaaggctt gaagtgggtg ttcatctcaa gaccgcaggc actacctggc tcgaagagat
     5041 tgttggcctt gtcgaagcag gaggcaaagg cctttcggtt gcaaagagcg tatatgagca
     5101 ggcctatctt cgaattgaag aacttacggc tccctatgcc aatgtcgtcg aaatcgatgc
     5161 ccagatgcta cccagcccca aaatggtggc cggttgggat tcggatactt ttgttgctgc
     5221 tttgcgtcat gagccaaatt catcgacgat gcagcctagc atgagacaac ttatgcatgt
     5281 gggattcaaa atcgcagccg aaatgggtag agaatatctg gatgctctcg aagaatgtcg
     5341 cgaatctgtt tctcgcaacg taaggtataa tctatatgca cgtcatctcg cgccgatcat
     5401 cttcggctga ttttccttcg aagggcaggc ttggctttca tcgccctggc attatgggaa
     5461 agatcctcat atgctttggg atcatcatta tcttttttct ggtgcttgcg cttgtcatgc
     5521 agaatgaatc gatgaatcta tcgcgcgaat atagcaagaa tctttcaaat tatctgctcg
     5581 tgcatcgatt caggctcaat ctaaactcat ttcattctca ggcggatcga tatcttcgtg
     5641 aaccagcttc ggtagaaatc aactctatct atgatggcat attgctcttg aattcacagt
     5701 atgtcgagct tcttccaatt gcagatattt cgatatatgt tgagttcgag gtgcgggcaa
     5761 ccggctatgg tctagatgct tatttccctt tagtctcacg ggcagttggg ctgagagccg
     5821 ggaattcgcc agactattat caggtctttg tcaatgcaag ccggattcat ggttatatag
     5881 atatatatct caacagaatg ctctccgagc ttatgaaaaa tggcgaggag acctatgtca
     5941 gcctctcaca taaggcggag atcctgaatc ggactatttt cttctccatg atttttgcaa
     6001 gcgccattgc cataggggtc attctttttg taactcaagc aattaccgca cctctgcgaa
     6061 ggctggcaaa agaagctgaa aagcttgcca agggcgatct ggatgcgggt attgtagaag
     6121 tgcatacggg cgatgaagta gagactctga cacgttcttt tgcgacaatg gcaatgagca
     6181 tcagggagta tgtggaggga ctcaaggaaa agtcagaact cgaaaggttg ctgcacgaag
     6241 aggagcttgc tcttgtgagc atgggcaaag cacttcgtga ggctcagttt ttaaaccttc
     6301 aggaacagat gaaaccacac ttcttgttca atgccttgaa taccattgct cgtagcgcgc
     6361 ttttagaaaa tgctcccaga accgaatctc ttgcaatttg cctggcgaca ttattgcgcg
     6421 caaccataaa ggatagcggg gcgctttcta cattagatga ggagcttgta atagcgcaag
     6481 cttatctcga attccagcag gtgcgttttg gagataggct tcaatggaat atagatgtac
     6541 cgcggtcttt gcgcaaaact agggttccac gtctgatgat ccaaccgatc gtagaaaatt
     6601 cagttagaca tgcgctagag ccaaaggtca gcggtggctt catatcgatt aaggcacaga
     6661 aacgcaagga agggcttgta ttgtggtttt tggataatgg cattggcatg tcgaaagaac
     6721 gccttgctga tgttcgagca aagattgcgg cttctcttca agtgcagagc tttgaccaaa
     6781 tcaaccttcc tcaggtcgca gaaattcctt tcagtgagga aaatgatgtc aatgaagagg
     6841 aactgctgga aggaaggagc ataggccttg caaatattgc gacacgcttt ggaattctct
     6901 atggagatag ctgccgcttc gagataaact cgagaaaaga gaagggcact cttgtgcgaa
     6961 ttactctgcc cttgggggaa aaggtgggat gattcgaatt ctggttgctg atgatgaaga
     7021 actcgaaaga agagccatga tccgtatttt gaattccata ccatcaaatg agattccaga
     7081 tgtaatggaa gcttcaaacg ggatcgaagc cttggatatc ctacaatcgc agaaggtcga
     7141 catcgtattt ctcgatatac atatgccagg cgttgatggg cttaaggttg cggaaaagct
     7201 atcatttctt tccaacccgc cagtaatcat aatggtgaca gcctatgact atttttccta
     7261 tgctcgccag gccttgagat atggtgttat agaatatctt cttaagccgg catcgacggc
     7321 tgaagtccat aatgctttat ttctagcgct tcgcgagata aaggctcgga aggcggaaga
     7381 ggagaggcag gcggaagcaa agcgcgtaac ggctggattg aaggactttc tccaatcaaa
     7441 agtctgtgca tctctgagcg aaaacataat ggataccgag atcatccgac agctagtaaa
     7501 gctggagacg ggaagcgaga cttggctctg catagctcta gtcgcaggga tgaaaaaatt
     7561 ctcgtctgac gatgtattat ctttggcgtc atgtgaattc caaagaacct tccctgttct
     7621 tgctgagaaa tttctactgt ccgatatcgg gcttgcaaaa gccaatccaa tgttattcat
     7681 atcacccaga gggttgagaa tggaagcaat ggcagatgag caaatgccgg atcccataat
     7741 taatgtattg atcatcatcc cccatgagat acaaaaagaa gagggcaata agattttcca
     7801 agagctcttt ttgtatagat acagtgagca ttttgaagac caaagcattg ggcgcttcct
     7861 ttgccgctgt cgagatgcag gagctaaaac gcttaggata ggtatcagtg cagcggacta
     7921 tcgttcggca tcgatagccc tgaggatggc tacaaccgct ttcaatctta gctctgtaga
     7981 acgacccatc atcgtattga atccgataca aaccgatgag ccaaaagcca aggacactca
     8041 taaaatagca agtattgcat tgttgtggat cgaggatcat ttcatggaga acatcggtct
     8101 caaggatttg gctaaagcca tcaatataag tccttcccat ctttctcgcc tacttatgaa
     8161 ggagacgggg attagttttg gggagatcct ctccagaatc cgaatagcac gagcgaaaag
     8221 cctgctcttg aatggcattt ccgtaaaagt gacctcattt ctcgttggct tccgcgatca
     8281 aagttatttc acaaaagtat ttaccaaaat cgaaggtatc tcgccatctc gctatataga
     8341 acaattccaa tctagatcta actaggttct tctcaagcac catattgcta ataatcagca
     8401 cggaattacc agacaaaaag ctaaaaagtg cttcttataa taatgagttg caggtatatc
     8461 tctaatatct gcaaaagatt gaattctcaa ggaggttgtg cgtatgaaaa aattcggtat
     8521 tattgcgctt gccttgttgc tcattctctt gccatcaatg ttgtttgctc aagccaagcc
     8581 gattgtattg aggcttgccg aaactcaccc caaaggctac cccacagaaa tgggcgatga
     8641 ggaatttgct cgccttgtaa aggaacgctc aaatggtcgg attatagttg aagtctatcc
     8701 cggttctcag ctcggcgagg aaaaggctgt gatcgagcag gtgcagtttg gagccataga
     8761 catgactcgc gtatctataa gccctgtcgc tgcatttgtt ccaaaactca atgcgttcca
     8821 gatgccttac ctttacagag actctgatca tatgtggaaa gtattgaagg gcaatattgg
     8881 catggagctt cttgcaagcc tcgaaccttt tggcttcatt ggtcttggat ggttcgaagc
     8941 aggagcgagg agtttttaca atagcaaaaa gccggtatat gcgccttcgg atctgaaagg
     9001 catgaagatc agggtgcaag agagcgagct aatgctgggg ctagtgcgtt cgttcggcgc
     9061 aataccaaca ccaatgcctt atggcgaggt atattctggt ctgcagacag gagttgtcga
     9121 tggagcagag aacaacccgc catcatatta ttctgcaagt cattatgagg tagcaaaata
     9181 ctatactctg gatgagcata ccatggtccc ggagatcatt attggttcga agatttcttt
     9241 agggagatta tcgaaagccg atcaggattt aattaagcag gccgctatgg atgctatcga
     9301 ttatcagcgc gctcaatggg atgcttatgt caaggtttcg atggataagg tcaaggctgc
     9361 aggttgcacc atcatcccta tacccgacaa gacagaatgg atgaaggcgg tggagcctat
     9421 gtacaagcag caatcaaaag agattcaaga tcttgttgct agaattcgcg cggtaaagta
     9481 aacatcgaat tccgctggtg aaacagtcga gatgcgcgca aggttagaca tcagggtttg
     9541 tcgagcatag atgtagcttt gcgcgcattt cgatatagtt ttctatgcct ataagctgag
     9601 gtttatcatg aaaaaattta ttgtctccgc agttacattg attcacattc tacttataaa
     9661 tctatcaatg atcatgattg tggcgatgct cttattggta tttgtaaacg tggttctgcg
     9721 ctatgtgttc aacagcggta tctattggtc ggaagaggtt gcgcttgtgc tggaagtctg
     9781 gtttattttc ctttctttgg ggcttggagt aaagtaccgc ctgcattttt ccataaatct
     9841 cgccaaccgc gaaaaaatct ccgcctctct aaatcgcgct ctggatattt taacggacat
     9901 catctacata tttatcggcg cggttatgat tatatatgga agcaagttag taagatttac
     9961 tatgagttcc gttatgcccg caactatgct gccggcagga atcctctacc ttgttttgcc
    10021 gctttcaggc tttgtggcaa ttgttgaagc tgtttttcat gttttcgggt tcaacatgtt
    10081 cgatgagaaa ttagaagcat acttttctgg tcaaggcata agaatcaggg acgtattgag
    10141 gagctagggc tatggatgtg actattccaa cgataatact catgggaggg tttgtccttc
    10201 tgcttctgtt gagagtaccc attacttttt cgcttgccgc agcctctatc gcaaccgcca
    10261 tctacctgca tataccgctc atggccatcg tgcaacagat ggtacaaggt gtaaagtctt
    10321 tctctttgct cgccattccg ttctttatca tcgctggcga gatgatggca aggggaggca
    10381 ttgcggaaaa aatcataaac ctcgcgaatg tcctggtagg aagagttcgc ggaggtcttg
    10441 gcatggtcaa tgtagtagat agcatgttgt ttggaggtat ctcgggctca gcggtagccg
    10501 atgttgcctc aaccggagtc attatcattc ccatgatgac taagaatggc tacgacaacg
    10561 acttttcggt tgctatcact gttacgagcg caactcaggg tataattatc cctccgagcc
    10621 acaatatgat catctattcg gtggccgcag gaggtgtgtc tgtagggagg cttttccttg
    10681 gaggctatat tcccggtatt ttactcggta tttcgcttgc agttattgtg tattccattg
    10741 cggtaagaag aaactacccc aaggggagaa agtattcctt taaggaaggc atcaagattg
    10801 ctatcgatgc tttttgggga ttgttagtgg ccgtgatcat aattgtcggc gttgttgcgg
    10861 gtgtttttac cgcaacagag tctgctgcgg ttgccgcagt ctatgcattt attattacat
    10921 tctttgtata taagcagatt ccactcagag aattcagaag cattctctac agtactctca
    10981 agacgctcac aatggtcatg actctcattg ctacggctag ttctttttct tggctcatgg
    11041 cttacttgcg aattcctgcc atggccactg agattttgct ttctatcacg gacaacaagt
    11101 atctattgct ctttcttgtc aatgtgcttc ttctcttcct cggaatgatt atggatatgg
    11161 cgccactaat tctcattgta acgccaatac tgtatcctgt attgattggt aaatttggaa
    11221 tgtcgcctat ccatttcgga ataatgttga tgatcaatct ggccataggc ttatgtacgc
    11281 cgcccgttgg ctctgccctt tttgtgggat gtgcaattgg caagatatca atagaaaaag
    11341 ctacgaaggc gatgattcca ttctatattg caatgattgc aacactgttc ttagtaacct
    11401 atgttcctga gattacgatg tttctgccta atctcataat gcctggccag taaagtggaa
    11461 tacttcggta aagctttagt ttatattgaa atataaggag ttgtcgatgt cgtttattga
    11521 atcgatgttc agtctggaag gcaaaattgc tgtagtaacg ggtggaggag gcatgctacc
    11581 ctcttatatc gctgttgcct ttgccaaggc taaagcaaaa gtagttcttt ggggccgtgg
    11641 aacgcatcat cccatggaag atgccgctag gatggtcaga aagcaagctg atttgcctat
    11701 agattgctct gatgtcattg gtatcacggt cgatactggc tctacagacg cttgcgaaca
    11761 ggccttggaa caaacaatcc atagttttgg cttacctgat attcttctca acggcgttgg
    11821 tggaaatatt gggagatcga aatttgttga attagatgaa aaaatcttcg aggaagtgct
    11881 aaagctcaac cttatggcag gtttggtcat accatcgaag gtctttgcaa agcgatggat
    11941 cgctgcaggc ataaagggct gtatgatcaa catgacttcg atgacaagct acaagcctct
    12001 gtccgacacc tgggcatacg atgcagcaaa ggcgggcgtg ctgaatctaa cacaaggact
    12061 ggcaaaggag tttgctcctt atggcattcg agtcaatgca attgctcctg ggttctttat
    12121 tggtaatcag aaccgtgccc ttctaataga tgaagagaca ggcgatctaa cgccgagagg
    12181 taaagcgata attgcacgca cgccatttgg ccgttttgga gaatacgaag agctttgggg
    12241 aactgcgtta tacctcgcaa gttcgaaggc atctggtttc gtaacgggag tgagcattcc
    12301 cattgatgga ggattcctca tcgacaacat ttagatctac aatacttcaa tctgtaatat
    12361 ctcaatcgac ggtaatttaa acggcaaaat aaaaggggga gtcatatggc tgattttctc
    12421 gatgaggatt ttcttttgaa gaatgagact gcaaagcggc tctttttcga gattgcacga
    12481 gatttaccaa tcttcgatta tcattgccac ttgtcgccta aagccattgc agaagacaag
    12541 cccttcgatg atttagccaa gatttggttg gaaggagacc actacaaatg gcgcataatg
    12601 agagcaaatg gtgcgcctga atcgctttgt gcaggtgccg gagcttggga tgaaaaattc
    12661 cttgcttatg ctggcgctct cactaaggca gctggcaatc ctcttgtcca gtggtctcat
    12721 ctagagctca aaagaatatt cgaaatcgat gaagtgctca caccgcaaag tgcattgagt
    12781 ataagagaaa aggcaaatcg aattctttcc gaaaagagca ttagcccgcg ttctctcctg
    12841 gaacacttta aggtgagcgt attgtgtaca acagatgatc ctgtcgatac attggcttgg
    12901 catagagcga ttgcagagga gcaactatcg ggatcttcgt tcaagaccaa gatattgccc
    12961 gcttttcgtc ccgacaaggc catgaaaacc gaggatatcg agacttggaa tgtctatatc
    13021 gattcgcttg gcgaagcggc tggcatagaa attgattctt ttgcaagtct tgcggatgct
    13081 ttgaccagtc ggcatgctta ctttcactct atgggaagta gactctccga tcatgctctt
    13141 cttgttccgc cttttgtgca agctatcgat accgagcttg attccattgt aaagaaaggc
    13201 cgaagtcgaa tagcgctatc gcagatagag cgcgcaaagc ttgaaactgc actgcttttg
    13261 cattttggca ggctaaatgc aaaaaaggga tggacaatgc agctacatat aggagctctc
    13321 aggaatataa atccaggatt gaatagggtg tttggtccgg atgtgggtgg agattgcact
    13381 tccgatgcgc ctattattgc tcctcttgct tctttgcttg agacattaga gagagaagga
    13441 aatcttccga agaccattct ttattcgcta gacccttcaa aacataatcc tctggctgta
    13501 cttgcgggta gctttgcagg caccgctcct gagattagtt cgccacaggt cgccggaaag
    13561 gttcagctag gcgcagcctg gtggttcaat gaccagaagg atggtatgga acgccatatg
    13621 aaggagtatg ccggagtcgg attactgggt gcgtgggttg gaatgctcac agattctcgc
    13681 tcctttatgt cctttccaag gcatgaatac tttcgtaggc ttctatgcaa tataatcgga
    13741 gaatgggtcg aagctggaga gctgccggat aatccactct atacagaaca gcttgtgcgt
    13801 ggcatttgtt gggagaatgc tgttaattat tttggaatgc tttgaaatcg gcgtcagggc
    13861 attcggagga taaaaaatca aaagggctgt ccagatcaat ctagggcagc cctttgttat
    13921 tcttaggaat atatctggtt tgccatatcg tgttgactaa gtaattcctc tgagattact
    13981 tctctgtttc ggtcatgccc gctatgaact gtttctgcat gagcactacg actatgaccg
    14041 gaggaagcat ggctagcata gtggtcgcca tcactatctg ccattcgatt tggacgtctg
    14101 caccagacat cattcggttt atgccaatta gcagggtata gaattccggt ttagtggtta
    14161 ttagcaatgg ccacaggtat tggttccatc cataaataaa ttggatcaca aacattgcag
    14221 cgattggcgt acgtgtcatc ggggcaagga tatggctaaa gaactgcatg ggagtcgcgc
    14281 cgtcaatctg cgatgcttct gctatctctc tgggtactga catgaagaac tgcctaaata
    14341 agaatacagc agttgctgag acgatcattg gcagaattaa gcccgcatag ctattcagca
    14401 atcccagatc tgcaactact ttatatgtag gcataatacg cacttctacc ggcaacatca
    14461 gagtgataaa gatactccag aaacaaaagc ccctcaatgg aaagtcgaaa aatacaattg
    14521 cgaaggctgc cataagagaa attactatct tgccaataga tataccaagc gccattataa
    14581 gcgaattctt caacatggta aggaccggtg tgcccatgtt ctgcgttcca tgcaataacg
    14641 cctggcggta attctcgatt agatgaggtc ctatgccgag aggcatgggt gctgcttgta
    14701 tatctgcatt tgtgtgagat gaagccgaga aaataaaata aatcgggaat atgataataa
    14761 ctatgccaat ccagaggatg atatgtggat aaaggctgcg aaggaccttg gctcggtgag
    14821 cggctttatt agatagctgt tgttcattca ttgttgtttc ctccgcttta gtacgtgact
    14881 cgccgctcta tgaagcggaa ctggaatacc gtcagaacaa tgaccattat catcaggata
    14941 accgattgcg cagaagagct tccaaggtca aggtttataa cgccatctcg ccaaaccttg
    15001 tagacgagta tagcagtgga cttccccgga gctcccgatg ttatggaatg cacaataggg
    15061 aaggtttcga agaatccata taccagatcc ataaccaaaa gatagaaagt agttggcgaa
    15121 agaaggggga atatgatttt ccagaatctc ttggtcggcc cagccccgtc aatctccgca
    15181 gcttcgacca gagttgcggg gacgctctgt agtcctgcaa ggaagaatac aaaattgtag
    15241 gccagttgct tccagctcgc ggccatggtg agcaacagaa aggcttggtt aaaattcaaa
    15301 agatactgcc aatctatatg gaaggtatgc ttaagcgtgt aagcgatgac acccacgtta
    15361 ggattgaaca tgaaaagcca aatgactcca gcgacgagtg ttgatacagc atatggccag
    15421 atgagcattg ttttatagaa agaagccaat ctgattttct tgtttgcctg gattgcaaga
    15481 aaaagagaaa tagccattga gcttatggtt atgcatagcg caaatactgt cgatacccag
    15541 aaagattgcc aataatcggg atcaattaga agattggaat aattctcgaa acctacaaag
    15601 atcatcttgc caccgaaagg atcttgcagg tagaaggatt gcagaatggc ttgtgcagaa
    15661 ggccagaaga aaaagataaa gacgattaac atctgtggta gcacaagaag atacggcaaa
    15721 attgaagctc tgaactcata ttgtttggac ataacagttc ctgaagaatt tagattatta
    15781 tactaaacca caaagaagtt gcatagcgcg cagtcctttt ctatatctgc gcaaaaaagg
    15841 ccgccgaccg gcggcctttt attacaaaaa catctccttc ttgtccactc tgagattctt
    15901 aaagttcaca gtgcggaatc aaatatctat ttattaagtt tctcaaaact gcgcaattgt
    15961 ttgtttcctt cacgtaccat cttgtcgagg ccatctttta ccgagatttt tccggcaagg
    16021 atttcttccc aaacctgatc ttcgatttcg cgaattatgg gcatatttcc aaagcggata
    16081 cccatcgagt taggagtagg tgtcttgttg gtcagctgct tgattgctac ttcgagtcca
    16141 gggttgttga catagtaacc ttgagatttt gtaagctcat aggctgcttg ggtgacaggc
    16201 agatagccgg tttctttatg ccaaagagct tgaatctcgg gcttggagat gaaattgaag
    16261 aactctgcaa ctcctttgta ttctgctgcg ggtctgccat tgaataccca gagagaagca
    16321 ccgccaatgc aagaattctg gggaacaccg ggtacatcgg gataataggg caagcgcgca
    16381 acaccaaagt cgaacttgca ggttgccttc atgttcccat agccaccgat cgactcgaag
    16441 tgcaggccaa cgcggccaga ggtgaagagg ggattggctt tattttcgcg gccgccataa
    16501 atgaatacac cggtcttgga aaggtcgtaa atgttctgga aatggcgcac cacgatagga
    16561 ctattgaata caagctgggt gtccaggcca tcgaatccat tgcttctggt tccaaatgga
    16621 atattatgcc atgcacagaa attctcaagc tgaatccagc ttatccagtt ggtcgtaaaa
    16681 ccaccttcca tgccggaagc ctttagtttc tttgcaacct cgaagaattc aggccaagta
    16741 acgggtggtt tttcaggatc cagccctgcc ttcctgaatg catccttatt gtaatacata
    16801 acggcggtgg agctgttgaa tggcatggaa atcattcggc catcggaagt ggaataatag
    16861 gatttgatgg ccggaatata gatatcggag ctccaattaa ttttattctc agccataagc
    16921 tggtagaccg gttttatggc attcttggca gccatcatgg tagccgtacc gacttcgtat
    16981 acctgaataa ttgcgggcgc ttgcccagct ctgaaagcgg caataccagc attcattgtg
    17041 tctacatatg atcccttata gacggggact actttgaatt gcttttgact tgcattgaat
    17101 tgatcactga tgatctgtac catttcgcca ttatggccgg ccatggcatg ccataattcc
    17161 accgtcgtct gggcataagc tccgacagcc attagtagaa cggctatcag caaaacgact
    17221 cttttcatta ctctgcctcc aggattatta tggattgccg gatctgcttc cggattctta
    17281 acggaaaata ttatattata gtttttttga gcacgcaata gaaaacaaat ctaggaatcc
    17341 tctaacaatc tcacttgacc aattccaagg ctctatcggg gaaatcggtt ataagtccgg
    17401 tcgcgcccca attcagaagc attctcattc ttgaaggatc gttcacggtc catggaagca
    17461 caccaagccc tgcggatctt aatgctctga cagttgcttc atctaggtat ttttcgttag
    17521 gattgagagc caccgccccg atatccttca atctctgcac agggtctgag attggcttat
    17581 cgattagggc agcaacactt atctctggga tcgccatctt cattcgcttt aagtaatcat
    17641 gattgaagga ggaaatcaga actgaaggaa ccatatcgag ctcttttatc agttcggctg
    17701 tcttctcgac aatccaagca tcgcaggcaa atccggttgc atctttgatt tcgatattca
    17761 ctcgccaccc cagacgcttc gtcaattcaa gcgcttcgcg cagcgtggga atccttagat
    17821 ccttgaagga ttcaagctca gaagacccaa ctcttccata ggcaatttgc ataaaaggat
    17881 ctgtcttgga gtaccatgag cctgcgtcga gggatttgag ttcatcaaac gtaaaatcgt
    17941 aaataatcca tggcgaacga gatggaaagc gagtcttagc atctgtcgtt cgtgtaagag
    18001 tgttgtcgtg caaaacgacc aatataccat cgcttgatgc cgcaacatcg agttcccaca
    18061 tatcggcgcc tacgtcaaaa cccttctgag ctgcgagaag tgtattttcg ggagcaatac
    18121 ctctaaaacc gcgatgagca atgacgatag gacgataaga ttgcatgata agtttctcca
    18181 tagataaact cttagggatg ttctgaccaa atagcgcaag gcctgcgccc ataagaaaaa
    18241 agaccctcag agccttattg cgcaatcggt tgcagggttt gatattcatt ttattgatta
    18301 taaagatctc gcggaatttc gacaagggaa agttgcacaa tttcatagag agcttgccca
    18361 tctaagccat ccagaatagg atgctagcgg agaataatat ggttgcaggc gatggaaatg
    18421 gtggacgcaa atggatggaa atatccactc gcaaagtcta tgaatgcaat gtgttcagcg
    18481 tattggagag agaaagcaaa ggccccgatg gacggacagg ccatttttcc ttgatgagag
    18541 cccaagattg ggcggtgatc gttccctatg tgcgaacaga tgccggcact tcgtttttaa
    18601 tggtgcggca gtatcggcat ggagcggagg agcttagctt ggaattcccg ggcggcgtgg
    18661 tggagaatgg agaagagcct tctcatgcgg ctgccagaga gctagctgaa gaaactggtt
    18721 gggtttcgga taagatcctt catgcgggta ccgtatttcc aaaccctgcg attcaggata
    18781 atcattttca tgtttttgtt gcgctggatc caaaacctgc agtcgatcgg cacctcgatc
    18841 aaaatgaaat catcgacgct taccttgtag atgcagatga agtacgacgt cgcatcggag
    18901 aaggggagtt aaggcacgct cttatggtga cagccctata ccttgcagac agattagttg
    18961 gctctcttaa agcctagcga ttgaagccta gcgagaccta ggaatcatag atgtcttttc
    19021 catgtgagtc gatagctata atgacgggca gtctatcaat cactgcgata cggacagcct
    19081 ccattccaag gtcttcgaac tgaattgtat ccagtttttg taagaagcgc tctgcattga
    19141 gcgcggctac tcctccgatt gcagcgcagt atacgccttt ataagtggct atagctctac
    19201 gcgcctcatt gctacggctt ccttttgcga ttgtaactat cgaagcaccg actttcataa
    19261 aagcttcaag atagctatcc atcctcgaag cagaagtagg cccaaaagcc ccagtcttgg
    19321 ctccctgctt agcctctgtt ggtccggcat agaataccgg taatccgatg aaccaatcgg
    19381 gaagtttttt gtcagactcg agcatgcgca aaaggcgcgc atgagccata tcccgcgcta
    19441 aaagaacagg tcctgaaata agggccatcg tacccgcttg caatgacctt aattttgaaa
    19501 gccagctatc atccgattgc gttatttgta taatctcggc cttggcgctt gtctgcagaa
    19561 tgatctcttg tgccttattt tctaagcatt gtgaaggagg tgatagtttc tcgatatacc
    19621 atcccgaatt atctataaat acagagacat gccgatttgc tgcacaggac actgcgatcg
    19681 ccaaaggtag atttgccgca tgccgtgata gtcttattgc tcttgtggag acggcaaaat
    19741 gcgaacctcc ccactgagcc ccaaatccac tatcccgagc tattgtcagc attttctttt
    19801 cccagagaat atctctcatg ggcataccat cgccattagc agaggtagga agattatcca
    19861 gtaggcctaa ggccgcgagt tccgcagcat acaaagactg tgcaggattt gtaccgccga
    19921 gcaccatcgc aatcgcgtaa ggagggcagc cggaaggacc caatgcattg atgcgcaaac
    19981 gcagaacatt ctccagttta tctgctctca gaatagcggg gctctctatg ctgatcgaag
    20041 agcgagaggc ggagcctcct ccctttgcga caaaggtgag cctaagttcc tttcctttta
    20101 tagaacgaat ttggatacct gcgggcagat tgtcctttgt attacactct tccatcatat
    20161 ttagaggacc catctgagag ttgcgcaatt tctcctgcgt ccaggcttgt gctgcgccct
    20221 gaactagtaa ttcttccaag tcaatgtttt tctcgatttc tatgcggtca ccgcgccatc
    20281 catagacaag cgcggtcccg gtgtcttggc atatcggata tcttctcttt ccagaaatca
    20341 gtgcattttc taaaagacaa gatgcgacaa atcgatcata ttcgctagcc tcaggatcct
    20401 cgatgatctt agcaaaagca tcatattgcg tttcaggcat aagataagct attttcgtaa
    20461 acgcaattcg ggagcactcg cgaagcgcat ctgcttgaag taccaaatag ccattctttt
    20521 ccataggagc gatcaaatcc atagtctcca aatcggataa gttcccaaag cgaattaaac
    20581 catcaagact gacatgagcc actctttttc ctcctttgcc ggctttgcta gccatgaata
    20641 gcttttcata tcaaaggcca ggcaagcgtc gatgcacaat acggaccggc gcgcgtccta
    20701 cagtaattgc cttggccggg catacctcat ggcagcaaaa gcaagtgata cagagcttgt
    20761 cattgattct aatgatgttc gagccatttt tcctatcgag caataatacc tttgctggac
    20821 atatctcgat gcacgcactg caaccgatgc acttattttt atgaaatata ggcctaaggt
    20881 gtatcagctt cccggctaag gaacgaaggc ctctcggtat tgcgctgagt cttttgccaa
    20941 gcttgcttgt ataaggaagc cgcctaaaac cactatgctc tatttccgag actcttagag
    21001 ggtaagtcga aggttccgta atatttgcgc ttccagacgt tctttccatt gcatcaagaa
    21061 tataggatat atttctaggg tcatagccta cacactgaga cgcaatccag tctagctttg
    21121 caacactcct agatgccagg acaatgccaa gcgagtaggg tgttccagat ccagggcctt
    21181 gcccatccat tgcgatgatc gcatccataa aagtaaagca attaggaata gatagagcca
    21241 gatccacaag catctcgcca aaatcgataa ccgcggggta ttgaagatgc atcgcactct
    21301 ttgctgtccc agggataagt ccaaaaaggt ttttgattgc gcccgtatat gtcataaggc
    21361 catgattctt cagttttgga agatttatga ctaagtcaac ctcctctacc agtgatgtca
    21421 gagggatgtt cttgagcttc ttgccctctt tgacagcatg cggagaaacc tctcggaaat
    21481 ccgcccaggt accaccactt tgctggaccg catcgtagat accagaagtc tttgcagcta
    21541 acatgactgg ctgccatcct ggagaatcgc ccaccaggat ttcttttgcg cccctggact
    21601 ttgcaaatcg aatgaccgct tcgacaaagg caggattggt tgtaaccgcc tttgaagctg
    21661 gcgaagcatt caaaacattt ggctttatga ggattgtctt gccttgtaaa tcaggaagac
    21721 tcgcaatctc tgcgcatgtt tcaatcgttt tatccagctt ttcggcttcg taactctcac
    21781 atctgatcag tgcgacatcg gctctttccg gcttcatgta atattcctga gaccagagta
    21841 tagagaaacc acaggcttcc atcaaggcaa atcaagatga ataaattgca cttgcctaat
    21901 tcaattggtt gcacttataa tatttgatca tcagattgag aggttccgga taagaatgcg
    21961 aatcaagctg gatcaaagca actgggtata ctatataggg cggggatcga acctaggaaa
    22021 gctggtgttt tctgttggat gtatgcttat gttgcttgga tcgagcttca ggccagctcc
    22081 aggtcccatg accgcgcgtg gaggttccga cgtcagtgac tttgcaaaaa gcaatgagat
    22141 tgctatgcca gaaccagaag acaaattggt aaaatattct gtttatgaag taaagaaagg
    22201 cgatacgctg tcagagatag cagagtattt tgatctgacg ctggatacac ttatcagtat
    22261 aaataatttt aaatcagcca aatcactaaa gccaggtcag ctcctcaaga tacccagcga
    22321 atctggcatt ttgtatatcg cttcaaagcc aacaacaatt caggaaattg ctgattcata
    22381 taatatcaat atcgaccgtc tcatagaaac aaatggtctg ctttcagaga cgattccagc
    22441 aggcaaacct ctgtttcttc cggatgtcaa attacctgca gccacattgc gagaaatcgc
    22501 cggcacacta ttccaatggc cggtcaaagg tcgtcttacc tcctggtttg gctggcgtaa
    22561 agaccccttt acaggaagaa gatcgttcca caacgctatc gatatagcgg cgccctatgg
    22621 ctcgtcgata accgccccga tggacggcag ggtaatagag acgggttatt cgccgatcct
    22681 tggcaaatat gcgatcattt cccattcggg cggatggaag acattatatg gccatatgtc
    22741 cgaaattctg gttcaggaag gacagtatgt ctctcaaggt cgaaccatag gtagaatcgg
    22801 cacaaccggg tatagcacag gtccccatgt gcactttgaa gttataaaaa atggggttct
    22861 ggtcaatccg ctgaattacc taccttgatc tttacatgct ctcgaaaaga gacatatcga
    22921 aaatctggga acgtcttatt tgacgcatat acaaatatct gatacattgt ttcatcatga
    22981 tgatcataaa gggaataggc gtttctcccg gtgtagctat agctcctgcc tttgtgcata
    23041 tagatgattc tggcataata cctcagtacc atatagccat cgatgatgtg caaagcgaaa
    23101 tggctcgttt tcatgaagca tcgttgctag ccaaaaagga aattgaagcg ctgagagaac
    23161 tggcaaagaa tgaagcaggc gaagaacaag cagccatttt cgatgcgcat atcatgatgc
    23221 tggacgatcc agagtttctt gaacaaatag agcgttcgct caaagaaaag cagttcaacg
    23281 tggaagtggc gattctctct tttgagagcg agatggtcaa taggctttct tcatcgctgg
    23341 atcctcttaa gcaagaaaaa gtatccgata ttcatgatgt ggtaaggcgt ttgctaggtc
    23401 atcttttgaa aaaagagagg atttcgcttt ccgacatcca aaccgagata atcctcgttg
    23461 ccaaggacct cttgccatcc gaaatggtga tgatgtcccg gagcatggta cggggcatag
    23521 taacagagac aggaagccgg acgagccatg ccgctatcct ggcaagagcc ttcgaaatac
    23581 ccgctgttct cggtgtaggc ccaggcttta tcctgcaagt gagacaaggt caatcggtat
    23641 atatcgatgg cgataatgga actgttgtta tcgagcccga tgattcgatt atccagagtg
    23701 agaaatcggc gcgtgccgct cgcctcaatc aggaaaaaga gaacagagaa ctaagagatg
    23761 ttccggctca tacgaaagat ggcacaagca tcatgctcct agctaacatc ggtatgcctg
    23821 aagaagttgt aaatgccata gagcatggcg ccgaggggat tggactgttt cgctctgaat
    23881 tcttctttct tggtgggcat ataccgggag aagaagagca gtacaatgcc tattcgcaag
    23941 tggcaagatc catgcattgc aggcctgttg ttattcgtac gctcgacatc ggcggcgaca
    24001 aagtattgcc ggaacttgga gtcataggag aaaaaaatcc cctgctgggc tggagagcta
    24061 tacgtttttg tcttgaaaag acagatctct ttaaaacaca gcttagagca attcttcgcg
    24121 cctcaggtca ggggaaagtc aatatcatgt ttcctatgat tagcaacatc gatgaactga
    24181 tccgtgctca agctgttctg gccgaagcga aagaagaatg caaatcaaag ggctacaaga
    24241 tagacgaaga catagagact ggaatcatga tcgaagtacc aagcgcggcc atctgttctg
    24301 acgtactctc aagatcttgt gatttcttct cgataggaac aaacgatcta agtcaataca
    24361 cgctggctgt tgataggggt aatcaaaaag tagcttattt gaataatccg ttcgatatag
    24421 cagtgttgcg ccttatcagt atgacaataa aatctagtaa gaaggcaaat atcggcgtga
    24481 gtctctgtgg agagatggca gcagatccag ctagcgcggt gctcctagta ggattgggct
    24541 taagaagtct ctctatgtca gcctcagcta tcccagcggt taagagagcg ctgatgtcca
    24601 taacgatgga agaagcttcc tccctggctc agcaagcgct atccatgaat acagcatcgc
    24661 aggttactgc cttgctgaat tctaggctca atctataatt attcttatga gcaaaaaaaa
    24721 acagactgta gagcctcttg agatagactt gcagcaaaaa ggcgctttat cacaatcaaa
    24781 agagaaagaa gagtcttcac caaacattcg ctatgaaaac ttgcctcttg tggttcttgc
    24841 agggaggcct aatgtaggca aatctacgct tttcaatcga ttgatcggca agcggaaggc
    24901 aataaccgat gcgaagcctg gagtaacacg agatcctatc gaagaagaat gcgagctatt
    24961 ggggaccaac aaaaggatat gcctggtcga cactggaggc ttcaagcttg agcgtgaagg
    25021 cctggatgag cttgtagtgg ctagagctct agcctatatt cagagagcgg atctaataat
    25081 attcattgta gatattacgc agatcacacc ggaagatgaa gaattcgctg catttcttag
    25141 gccttatgcc aaaaagctct tgcttgtcgt aaacaaagcc gatagtcccg agcgcgatgc
    25201 cctggtatgg gagcatgccc gctggggttt cgggccgatg ttgtttatct cggcagaaca
    25261 caatagaaac atagacaaac ttgaagaagc cataacggcg cgtctggatt tctcgagcgt
    25321 tcggcaagcg gaagtagaag cagccgacat acgcatagca ataatgggta agccaaatac
    25381 cggaaaatcg acattgctca atgcgctggt cggctatgag cggagcatag tctctcctga
    25441 gccaggcaca acccgtgaca ttgtgaaaga ccactttcaa tatgagggca ggggctttga
    25501 ggtgctcgat accgcaggaa ttcgaagaaa gaagaaagtc acagacaaca tagaatatta
    25561 ttcggttact cgctccatac gtatcgtgag ccaagcagat gtcgtcgtgc tactcataga
    25621 tgcggaagaa gggctttccg accaggataa aaagattgca gcctttgctg tagaagcagg
    25681 ccgaccggtg gtgtttgcgc tgagcaaatg ggataagatg ccgaacatga agaatgcgtt
    25741 cgaagctacc cgcgaccgct tgcgattttt tttcggacaa atggcatatg cgcccatcgt
    25801 gccgatttcc gctaaagaaa ggaaggggct cgacaaactc atgtcggtgg tgatctcctt
    25861 atatgctaag gcaaacaagg tgatcgagac ttcgcatctc aaccaagcgg tcgcggaatg
    25921 ggtcaaggaa acaccacctc cagcaagccc aagcaatcgt ttcaaattgc gctacgcggt
    25981 gcagagttcc gtaaatccgc agcgttttat cttttttgta actcggccag aagcggtacc
    26041 ggaatcctat aagagttttc tcaaaaacaa gataaggctc gattttggtc ttgatggtgt
    26101 tccaattcag ctggagcttc gtgcttcacg caggaacaga cagcggagct gatcgatgat
    26161 atacctgagc atcggacagt tggaaaagat gttgtcggta ccagcatcca ctttgcgatt
    26221 ctgggagcaa gaagtgcctt ttctgatgcc cagtagaaca aaatccggcc gcagatgcta
    26281 ctcgctctcc gaagtagtgc tcattgcaag gctcaagcat ctggcgctgg ataggggact
    26341 tgggcttagt tacacacaga gaatactaga gtacgagctt ctctatcagg atcaagagct
    26401 gagagcaaac atcaagcagt taagagaaga gttgtttcgc cttttgttgg aatgcgatcg
    26461 atggaaagtc tcattcaaag ggctgctgtc tgtttctgct ttagagaatt cgacaaagga
    26521 gaattcggaa aaggagttat tgaatggaag aagcgataaa gaatctgatg gatttcgttg
    26581 atgcatcgcc cacgcctttt caagccatag ataaccttat aggtattctt gaagcgcatg
    26641 gagctatcaa actggatgag agggaactat ggcagctaga gccaggtgtt tcctactata
    26701 tagttcgagg aggtagctct ctagtcacct tcagaatggg tctgaaacca ccttctgaat
    26761 gcggctttct tctcgctgga gctcataccg acagccctgg cctcaagatt cgtccggaaa
    26821 aggaactcca atctcgcggc tatctaaggc ttgcgctcga atcctatgga tcaccaattc
    26881 tgtcaggttg gctcgataga ccgcttggac tagctggtgc tctagcaata caagagaatc
    26941 aagagataca ccagcgttta tatatcagtc atgagcctat agcagtcatc ccaaatcttg
    27001 ctatccattt gaatcgcgat atcaacaagg gttttgaata caacctgcat cagcagttac
    27061 ctgctctctt tgggcttacg agccacaagc aaaaagagcc cgaagcagcc agtgaattcg
    27121 gaaaaacaaa agaaatctct ggagcttttg attccgaaat tttggtgcgc atttctcaag
    27181 agctcgatgt agaccctaag gcaataatct catccgatct gaggctctat gaccctgaac
    27241 ctactcgcat tttcgataat tcgctcatca atgcgccaag attggatgat cttgctggct
    27301 gtgctgcatt gatagaagca tttatcagtt tgcatgctca ggcttcgaca caagtcgcat
    27361 gtttttttga cgctgaagaa ataggatcaa tgaccaccgg aggagctcaa tcctcatatt
    27421 tgcgcgatat tcttgccaga attacgcttg cctcgcgtag tcttggccag gatttttata
    27481 gagcgctagc ttgttcagct tgcgtatcta tagatggagc gcaagcatgg catcctgggt
    27541 atccagataa attcgacgac ttttatacgc cgcttttagg caggggtatt gcattaaaat
    27601 ccaatgcaaa catgaattat gcaaccgatt ttacttctca gcaaattggt attagagtag
    27661 ctagcaaagc agatataaaa ccccaaatct atatggcccg tgccgatatt caacccggaa
    27721 agacgatagg tcccatcacg gcaagcagga caggcatccc tacagtggac gtaggccatc
    27781 ctctattggc catgcatgcc ataagagaga ccattgctgc ctcggaccac ttagacatga
    27841 ttgcttttct tcgggcattc tattcatagc tcgcggctcc atagattcca tagattccat
    27901 ggatttttaa gcataaaaag gccctcccaa aagatgaaaa aaggccgccc tttcgggcgg
    27961 cc
//
LOCUS       DAOMZL010000016        27339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_1408_length_27339_cov_14.318978, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000016 DAOMZL010000000
VERSION     DAOMZL010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 27339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27339
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1408_length_27339_cov_14.318978"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PLQ30_02525"
     CDS             <1..851
                     /locus_tag="PLQ30_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPW01348.1"
                     /translation="GLSIGSVYALMAVGYSLVYSIMNFSNFAHGGVIMLGAYFGFFCL
                     TLFKLPFALAFLLASLGTLVVAIIIERIAYKPLRERKAPFLYFIISAMGTSIMLENLV
                     IATIGPTFRTYPEVFPKVPIEIGNLAIGRLDLIIFVISAVSLTLLMIFIDKTKLGKAI
                     QAASYNIKACALMGINTDIVILTVFGLGGFLAGIAGVFFGMKYTVYPQIGNITTKSFI
                     AAVFGGLGSLPGAVLGSVILGVIETFVAGYISSQFRDLIAFVILIGVLVLRPRGIMGK
                     VTEEKA"
     gene            861..1793
                     /locus_tag="PLQ30_02530"
     CDS             861..1793
                     /locus_tag="PLQ30_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPW01349.1"
                     /translation="MDLTYIQGILMLAGINMITVLGLSLLTGFTGLFSFGHAGFMAIG
                     AYIGTWISGSPASTPAGLGLPFIFSILGGGIAAGLVSYLIGRITLKLKGDYFCIATLG
                     FGEAIRLIFNNVNRFGGARGWPGIPGRSTFLAILIADVIAVLVLANIVNSKHGRNMIA
                     VREEELAAQTAGIDVFRYKMISLVISAIYAGVAGVFLAHYMTFIQPKMFSLTKSTELT
                     IIVIFGGLGSISGSVLGALVLTALPEILRAFEIWRLVFYGAAVILIMVSRPKGLMGGM
                     ELTPSGIRKASAERNALREAKAKLLVEAQDGEEQ"
     gene            1790..2578
                     /locus_tag="PLQ30_02535"
     CDS             1790..2578
                     /locus_tag="PLQ30_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW01350.1"
                     /translation="MTSSPILHIKELTKNFEGLIAVDNVSFDVPEGGIIGLIGPNGAG
                     KTTIFNLITGIYKPTSGSIEFKGNSLVGLEPFRIAEKGITRTFQNIRLFKNLSVFDNV
                     LTACHRSANYNFAEAVFRLPRFRYQEKELKEKAEQLLDIMGLQCYKDLIANNLPYGLQ
                     RRLEIVRALALDPTLLLLDEPAAGMNPDETEQLMSLIGKIRDDFKLSVLVIEHHMDLV
                     MGVCENIVVLNFGEKIAEGNADEISKNPLVIEAYLGTPEEAANA"
     gene            2571..3293
                     /locus_tag="PLQ30_02540"
     CDS             2571..3293
                     /locus_tag="PLQ30_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW01351.1"
                     /translation="MLEIKNLHVAYGGIRALKGVSLSVQEGEIVTIIGANGAGKSTLL
                     NTISGFLRPAKGTILYKGKTLPRRPDLIVKAGICQVPEGRLVFANLSVLDNLMLGAYR
                     RNDKKAVRNDLKKVFDIFPRLEERKSQRAGTLSGGEQQMLAMGRALMTNGELILMDEP
                     SLGLAPLLVQTVFEIIEQFRGLGKTILLVEQNAFKALQVADRAYVLEQGRVVKQGLSR
                     NLAQDAAVKEAYLGSRKRNGGG"
     gene            complement(3296..3910)
                     /locus_tag="PLQ30_02545"
     CDS             complement(3296..3910)
                     /locus_tag="PLQ30_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPW01352.1"
                     /translation="MPDFRTNEPIAIILAAGCSSRMGATKPLVEIEGVPLLLHAARCF
                     QEAGIEEIIVVTGFDSDNVGKLASLHGIRPIFNKDYQKGMFSSICAGLRAVSSNCDSA
                     FILPVDIPFVLPSTVVKLKNAIKDKPIVIPRYEKVSGHPPIIHSALFSLVERWNGKNG
                     LRGFFGHRQADIEYLEVDDYNILSDIDTEADLRELLLKRAELRS"
     gene            complement(3950..5077)
                     /locus_tag="PLQ30_02550"
     CDS             complement(3950..5077)
                     /locus_tag="PLQ30_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HPW01353.1"
                     /translation="MRELRSWIAERLNSDQTVVISAITHASGSTARGTDALMAVDMNG
                     KMEGTVGGGYIENQAIIAIRNMLEAGYDHRDLFFDLSPEAQQNQITCGGKVSLHLERI
                     DQQSKARSALKSCLEQVESGNPCAFFVARTPGEKYVSAIDERGNVLYPHQQNSCEELI
                     AKSIIESSKKIQTCLDGARTLSGASCFELELPEKGISNIFQVFLLGFRPDPVVHIFGA
                     GHVGKATSEVASLVGFRVIVSDDRPELLTRERFPKANMLRIIDDFADPFTASKGAPAI
                     EIGPQDCAMILTRKPDIDEKMLSCLLRTRAGYIGLIGSKTKRDGIFAALRDEGFSETD
                     LSRVHSPIGLEIGAQTPEEIAISIIAEVIADRSGVLPKLKY"
     gene            complement(5082..7475)
                     /locus_tag="PLQ30_02555"
     CDS             complement(5082..7475)
                     /locus_tag="PLQ30_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPW01354.1"
                     /translation="MIDNNDPIKPMDSETLKATQKFNVVAHDIAKIDGEGLVLGRPAF
                     ADDLAPANALYVKLVRSPHAFARITSIDPSEALALPGVACVLTWKDCPRVPITRAGQG
                     NPEPSPHDRFILDEYVRYVGDEVAVVAAEDEVTAEKAAMLVKVQYEVLQPILDFEKAL
                     DNPIIIHPEPEIHEMFPIGFEPKRNIAAAYNMEIGNVEQELANSPVYVETTVHTQAQQ
                     HVALESHTAFSYIDIHGRLVIVTSTQNPWHTRRLLALALQMPIRQIRIIKPRIGGGFG
                     GKQHIHIEPYVAMVTMKTGRSARLALTRREVFEATFTRHEMRVKVRLGANSDGKLNAI
                     DMQVLSNTGAYGEHALTVFMVAGSKTLPLYNKVNAVRFGGHVVYTNKVSAGAFRGYGA
                     IQGLTGLESAMDELAHKIGIDPLELRRKNMIHEGETSDVFRIMGEGTEGVAMTIESCK
                     LEECIARGKELIGWDPNRLVWDVAPGKVRAMGMAIAMQGSGLPLIDMGSARIEFQDGG
                     FFKLHIGATDLGTGSDTILAQIAAEELGVDVENIIVYSSDTDNTPFDVGAYASSTTYV
                     SGSAVIKAARSLKKKLIEAVAQKFEVDPAFVLFDNKTFKTLNGGKSLSLYDFSFDTLY
                     HDGERMKTLEATESFTGAKSPPPYLAGFVEIELDKETGKVEVVHYVSVADVGTPINPR
                     LATIQIEGAALQGIGLALYEDVRYSERGHMLSHNLLTYAIPSREDVGKITVELVDSYE
                     PSGPFGAKSAGEIGIDAPPAAIGNAIRNAIGVRLTEYPFTPERVLMAIDRAKAEGAN"
     gene            complement(7468..7923)
                     /locus_tag="PLQ30_02560"
     CDS             complement(7468..7923)
                     /locus_tag="PLQ30_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPW01355.1"
                     /translation="MTITLYINGNEKILDCQPGDMLTEVLRKAGYVEVKKGCDTANCG
                     ACTVLIEDKPILSCSYLAVRADGKRITTIQGVAEEAKEFARFLTAEGADQCGFCAPGF
                     ALTVLSMKRELRNPSEAEIRHYLSGNLCRCSGYEGQIRAIKKYLEAVHD"
     gene            complement(7920..8708)
                     /locus_tag="PLQ30_02565"
     CDS             complement(7920..8708)
                     /locus_tag="PLQ30_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPW01356.1"
                     /translation="MEIREYARPKSMDQAMHLICEEHGTAIGGSAWLRTSTKTVMLGV
                     DLCDLGLEYIREFQDHIEIGAMTTYRELETSPLLDSRFGKIFRTTVSHIVGVQLRNII
                     TVGGTVAGRYGFSDLNTTLCAIGARVVFYPNVEKDLSSFIEKGYASPFLIEKIILPSS
                     ARAAYQQMRISESDFPIINTAVAWTGTVWRIAVGSRPAATRICRNAMKILGDSANPSD
                     EQIEAAAAETSKETQFGSDIRASANYRQKILPVLVKRALLEVRG"
     gene            8832..12248
                     /gene="dnaE"
                     /locus_tag="PLQ30_02570"
     CDS             8832..12248
                     /gene="dnaE"
                     /locus_tag="PLQ30_02570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPW01357.1"
                     /translation="MAEFVHLHNHSDYSLLDGAASIQKLIRKAKALGMPGIAITDHGN
                     MFGAMSFEKECRDFGLNPIIGCEFYVASGSRLEKTGTENGNRYWHLILIAENSTGYRN
                     LLKLTSASYTEGFYYKPRIDDELIAANSDGLIASTACLAGEIPSLILSGKLDQAIKRI
                     GFYKELFGKDHFYLELQDHGLEEQKIVNRELIGLSKKLSLPLLATNDIHYVDGEDAIA
                     HDILLCIGTNRKRDDPSRMHFPNDQFYLKSAEEMASIFAEVPEAIANTLVINEMAKLK
                     MEFPGPLLPNYQVPEEFSSPEEYLRHIARQGFQSRYPNPNAEAIHRLEYELDIIIKMG
                     FTGYFLIVWDFISWAKEHGIPVGPGRGSGAGSIVAYAMRITDIDPLKYDLLFERFLNP
                     ERVSMPDFDVDFDFERRGEVIDYVTHKYGSDRVAQIITFGTLKAKAVIKDVARALDIP
                     FDEANQIAKLVPEDPKMNLQKAIKKEPKLATLAQNPSYFELFSIAAKLENLHRHSSIH
                     AAGIVIGKTDLIDYVPLYKDPKTGLIATQYTMEYLEACGLVKMDFLGLKTLTLIRNTM
                     ELLRKRGIYLKEEDIPEDDPRTFKMLGEGKSTSIFQFESQGMQSILKRAKPSSIEDLI
                     ALNALYRPGPMEFIDQYIDSKNGKIAISYPHPSLEKYLKGTYGVIVYQEQVMQVAREV
                     AGYSLGRADLLRRAMGKKKPDILQREEAPFIEGALTRGYSREEAKRIFDILTPFAGYG
                     FNKSHAAAYSVIAYRTAYLKANYSVEFMAANLTNEISNTDKLTVYISEARQMGLSVLP
                     PDINRSEAKFTVDNGQIIYGFLGIKGVGEGIAMEIQKERESHGRFIDFLDFLTRMNNA
                     GINRKTMESLIMAGCFDSLKKGRHELMGNFERAMEYVHEKSSFEASRSGMLFQEDDLG
                     IYPDFVFQPFEEFSNQEMLQAEKELLGFFFSAHPMDEYRMIWERSSTLDLGHLDQAST
                     NREYIVVALLKEFRIYTTQNGRRMAFGKLEDYNGSIEMVVFPDLFEKNEASFVKDRVL
                     FLKGVLDDSRRDFSLKVQAILDAASLKNNSWRELHIRFKRAAIEEADLYPLRDALYSI
                     HGQCRVIFHVPMKGGAETLIEAGAHTKCSARDEDMAFIERQAAVAEVWRK"
     gene            12245..13081
                     /locus_tag="PLQ30_02575"
     CDS             12245..13081
                     /locus_tag="PLQ30_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW01358.1"
                     /translation="MKIKILGNGGFYNEGLAYNALAIDGHVLVETPPDILQSLHSQGM
                     KPSQIDTVYISHNHGDHCFGFPFFFFNWLYAGGSDSHYEKQTELVIIGPEGLKEHLKA
                     LMRLAILPEHQYLFDFDQRVKFVEIDERSIVSTSGGLWFGFLHTKHFVPTFSLVVGKT
                     GPDKGIPSCNDYLENAIFIYSSDTSMFDGIYKLLSSSARLILCDTNGEEDNGVHMSPR
                     ELMAAENQVRTRSSERADLSEYRLSGENDKDRVLGIHLSKKMDRAQGLKFAQPGEEYC
                     IE"
     gene            complement(13109..13795)
                     /locus_tag="PLQ30_02580"
     CDS             complement(13109..13795)
                     /locus_tag="PLQ30_02580"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPW01359.1"
                     /translation="MEGRYRLRAVAFDVDGTLYPEYSIALRCIDLFLSHPRIFMAFSE
                     VRKEIRFLQAQPNYIPRNMEELHSFQISLVAERLRKDELQIGQSIERIFYHLVPERFS
                     LIRPYKGTRQAIEAIRQRGITITALSDMPPQNKLKSLGLDDLFDFKLCTEDWGVLKPH
                     QRAFDGIIQTIRIQPSEILYVGNNVEYDILGAKKAGMRAALRGRQNSDADLSFSNWEE
                     LTEWVVKHID"
     gene            complement(13906..15675)
                     /locus_tag="PLQ30_02585"
     CDS             complement(13906..15675)
                     /locus_tag="PLQ30_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPW01360.1"
                     /translation="MENLIDITIDGQTIKVDSNENIVEACARAGVKIPTLCFLKDISE
                     NASCGVCVVEVEGAKSLLRSCVQKPTPGMKIRTSSPRVIQARKAAVELLLANHPDECL
                     SCLRADTCELHTMANLLGVHADRFPGYKKYPPPDNSSEGIVRDDRKCILCGRCVSVCE
                     DTQGVSAIAFTGRGTRTRVATFMDRGLAQSACVQCGQCSLVCPTGAITEKDESQDVYG
                     ALGDSRLSVVVQTAPAIRASLGEALGLPAGSLVTGQMVAALRRLGFSRVFDTQFAADL
                     TIIEEGNELLERLSHGGTLPMITSCSPGWINFIEGFYPDLLNHVSTCKSPQQMFGAVA
                     KTYYAQNANIAPKNMRVVSIMPCTAKKYEARRKEMDSAWDWWKEKDPETTGLRPFFDV
                     DWALTTRELARMIRLAGIDITALPQEDFDDPLGKSTGAATIFGTTGGVMEAALRTVYE
                     IVEKKPLQNMDFVQVRGFDSIKSAEVVLGGSPVRVAVAHGLSNARILLDEIRAGKSPY
                     HFIEIMSCPGGCVGGGGQPVLADIEKKRARSLSLYEEDRRLPIRKSHENPAIVTLYKE
                     FLGKPLGHLSHELLHTSYKARVF"
     gene            complement(15689..18883)
                     /locus_tag="PLQ30_02590"
     CDS             complement(15689..18883)
                     /locus_tag="PLQ30_02590"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW01361.1"
                     /translation="MNKNTDLGFLAQEGFSHFHGDTPWISVGMGTCGIGSGADKVWET
                     LSQKTENTNIRLRRVGCFGFCAAEPVVMTWRPGKPVLFFTEVNTSKASRIFAGLSSDL
                     GYEKIAKMAEAKIEEWDFRTSKVDFGEGYDFLPTWKDINFFRDQEKIILRDAGMIDPE
                     CIEEYIAVGGYGALVRALTSMPPEAIIEEVKKSGLRGRGGAGFPTWKKWSLMRKSLID
                     NPGEAYLICNADEGDPGAYMNRNEIESDPHMLLEGMLLGAYAMNVSKGIVYVRAEYPL
                     AVYRLNLAIETARQHGLLGKNILGSKFSFDIDLVTGAGAFVCGEETALIASIEGNAGR
                     PRPRPPFPVEKGLYGRPTSINNVETWCNIPVITARGGEWFAGFGTPMSTGTKVFSFVG
                     KVRNTGLVELPLGSTLESVIYGICGGMGPKRKIKAVQCGGPSGGCVPASLFKTPIDYE
                     HLNELGAIMGSGGMVVMDQDNCMVDVARYFTGFVSGESCGKCTSCREGTSQMLHILEK
                     VSDGEASEEDLDTLEELAIIVKDSSLCGLGQTSANPVLTTLRYFRDEYIEHIKGKRCP
                     AGVCENLYIALCESSCPLHMNIPGYLQLLKENRIEDAFELTLRENPLPGTIGRICHFH
                     CRMRCRRDMLDEPVSQGEIHRYLADTMYKMGREKQVYTKLLKEKLPPSGKKIAIIGAG
                     PAGLTAAFYLVRLGHEVTIYESRQEAGGVLRWGIPSYRLPKDILKKEISLIEKLGTRF
                     VFNTRITEPEQWKKLYEENDAIIVAAGASSELTMNIPGEDAIGVYKACDFLNALAKKE
                     KVHTGGDVVVIGGGNSAIDAARSALRLGATVRIVYRRSKADMPANLDELKGALEEGIE
                     LICMASPLEIMTQEKQGKHVARAVRIQRMKAGPIDSSGRPTPIPTDKIEDIPCSMVIM
                     AIGEKVEIPGIEVLGVERLKNGRIKADPFSHITSNPKVYAIGDAMLGPATAAEAMGQA
                     KVVAEIIDQALSGKKRFDSLFRCFEYRMEIPANTSKQKMTRPTMLPINARKGNFMEIN
                     LGYTGEQARIEADRCLRCDVREHRREPRGSLVGD"
     gene            complement(18880..19395)
                     /locus_tag="PLQ30_02595"
     CDS             complement(18880..19395)
                     /locus_tag="PLQ30_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPW01362.1"
                     /translation="MTKTISNSESLNVESIIDSYKGKNGGLLGALEQAQQNDPKKYLS
                     ETTLREISKRLDIPLSRVYSVATFYSFFSLKPQGEHVIIVCRGTACHTRGSLVLLNEA
                     LSRLRIAEFNEKEENSVTSSDNLITIRTVACFGQCALAPVIMVDGVVLSRMTVSKLVS
                     LLEKIRRGGGR"
     gene            19580..20515
                     /locus_tag="PLQ30_02600"
     CDS             19580..20515
                     /locus_tag="PLQ30_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01363.1"
                     /translation="MALNNREDEGISIVRPKWPAKFANQSVDCLGRKLKRIFDQNASS
                     DYFTSDEIRDYSYYERLLIQELEGLDQKISKGRLKPSYIAEYPRVIHADEGKIRACIY
                     IGSFDPFQLTHLTVALRFLASDLSNSDFVVIVPEGSPDTAKPLKTDYAFRYSIAKMQI
                     EGIFDSFIKALDLGMQADTIEIIRRFIAMHSGYTLELTHLIGSDALPIAASLISEDMR
                     IWRKEAQQSKVDYQHSIYVVQRGDIPEMHSYLKALKEEDIQIIIDPFSVEAPSSSDFR
                     TKQAYTIVLPTASIRERMEIIFRYHLHKSWSSDRQ"
     gene            20605..21261
                     /locus_tag="PLQ30_02605"
     CDS             20605..21261
                     /locus_tag="PLQ30_02605"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPW01364.1"
                     /translation="MNIQSMKLIFLGPPGAGKGTIARMASERLGIPQISTGDIFRTAV
                     KEKTELGLKVQSILASGALVPDELTIAIVKERLSWPDVRQSYILDGFPRTIPQAEALA
                     SFSKPDSVVDFEVDDGLILFRLTGRRVCRTCGAIYHIVTRPSATEGICDLCGGELYTR
                     NDDREETVKIRLEAYHKESEALIEYYSSQGILMVIDGSPDPETVYDTFISKIGRFLKK
                     "
     gene            21318..22724
                     /locus_tag="PLQ30_02610"
     CDS             21318..22724
                     /locus_tag="PLQ30_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW01365.1"
                     /translation="MFFILTIFSILPVKSAGTEEIVPGVWKGALVLREVAQEQSSPKL
                     PSYPKDQQSMSLSFEFSLKILSQDRGLLVDIPEQGLYSYPIDEYSFDTQQISFIFDAI
                     EGDEELLFVGTYSSSFLPPLSNHRGAIVGSVRGRSWNGSFYVQRQDSSVDPGSIFLDI
                     PIEDGALPATLVFPLSETPSLDPNSSSDFPLILLVAGAGRADRDGNNVDVPGKNDSLK
                     QLSNMLRMRNVGTLRYDKRGAGEAYKLEKPGRMTSFSEHVRDLSSVIVAAAAIPGHGR
                     LIVAGMNEGAWIAMAALNDERLASLVDGLAVIDASGQSPIETLKQSLAGLDEDLRKKA
                     LDAAQSLVQNGVLIDVPAELADFFAPSRKDWLATWLAFDPVETIAKVRIPILLIYGEN
                     DLQVSWQEFSMLVEARPEAAIRIVPRMNYMLKEVKSEDENYSAFTDPSFRVPSLLADL
                     LASFAKAQPAPQGFILWH"
     gene            complement(22721..23722)
                     /locus_tag="PLQ30_02615"
     CDS             complement(22721..23722)
                     /locus_tag="PLQ30_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01366.1"
                     /translation="MRFLPNNRKESRIKNTSIADIILLAAICISVVSCSSVPKEIAIA
                     GIFDRFEKTPHVILRSGDIFLRDIAACLDDSTIQAIETIASDKSNESEKKKEPSKPID
                     HANIDKLLTRAQVVGIGVSLTETGQPALEAVFAGNFPDLLTSLSFSLDSNWKKINGGY
                     ALKSGSLYVRNPNQGQVHLATWVPPASPITNPGASALAARSGVLANAMDFAIYIDAKS
                     TIVSQLPIMEGVTLPFEGILMSAIREKTSPPKGDPSAMYRTTFQIRMKDEQSARTYKP
                     IVRFLWVMLSSKLSSYGIAISPEIAIEQQGSIFISQPVMISARQLVDVGLSISTFDN"
     gene            23969..24418
                     /gene="ndk"
                     /locus_tag="PLQ30_02620"
     CDS             23969..24418
                     /gene="ndk"
                     /locus_tag="PLQ30_02620"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPW01367.1"
                     /translation="MAAERTFAMLKPGVLSRRVVGEIISKIERKGFKIIGLKMMNVTI
                     ELAERQYAEHKGKSFYLDLLEYIVSGPVVVMVLEADQAIKLFRLLCGFTSPEQALPGT
                     IRGDYAMHTSINIIHASDSKESAVREIDLFFRPEELIDWEDGNQKWI"
     gene            24500..26305
                     /locus_tag="PLQ30_02625"
     CDS             24500..26305
                     /locus_tag="PLQ30_02625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HPW01368.1"
                     /translation="MTSTVEVPCWNLDDVFRGFESEEYQKAKNELKSLLETLKRDLTD
                     AKPPSDAKGTANWLFELLSRIDRLETLADTLSAYVQARFTTDTRDSLVIQELGKVEEL
                     LVPATTILVLFRNMLARYEKRIKKAISHDARFVDYAFVLEEALMFQKHQMLPQLEDLA
                     ADLGRSGADAWSRLQDSILANASAVWNEATGERKTMVELRNLAYDPDRNIREKAYRLE
                     LSIWKTYEIPVAAALNGVKGTVLTLNKRRAWESAIDASLAQARISMATLEALIATMEE
                     SLPMWRRYLCAKARLLGLDRLSFFDLFAPLQNQGAVIQSYTWSDAKEFIVRQFGTFDK
                     EMASFAQRAFENRWIDALPREGKVGGAYCTHFPAAKQARVLCNFDGSYSSLITIAHEL
                     GHAWHYENIKDLPSLLSQYPMTLAETASIFSETLVSNAAMATMGEDARVPLIELHLQD
                     ACQVIVDILSRYYFEHDVFKERAKGEITPDRLSDLMLQSQAKTYGEPLREDERHPYMW
                     AVKGHYYIPGLSFYNFPYAFGQLFGMGLYSIYKEEGQSFAAKYRELLRFTGSLPAAEV
                     ARRAGFDIERPEFWQGAMKTFEHEVEFLEAQSNTR"
     gene            complement(26321..>27339)
                     /gene="lysS"
                     /locus_tag="PLQ30_02630"
     CDS             complement(26321..>27339)
                     /gene="lysS"
                     /locus_tag="PLQ30_02630"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPW01369.1"
                     /translation="EIDDWDGEWGLSYHCETCGFLEKGDLRTLKGAKLLWRVDWPMRW
                     AYEKVDFEPAGKDHHSQGGSFDTSKHVVEEVYGRKPPVTFRYDFIGIKGSPGKMSSSK
                     GKVVDLQDLLRVYQPEIVRYLFAGTRPNTEFVISFDLDVIKIYEDYDRTERIYWGVEK
                     AKNEEVEALERRIYELSQVDRVPEEMPYQIPFRHLCNLLQIHLGNIDAVLSLLPGLKP
                     SQKAHVERRAQCAWYWITECAPEDFRFFLRKPGELASLPEAEAAALRRLRDEVVEKLD
                     TYATENDIAEAIYAAAQSSNIEPKRLFIAAYQALIGKDQGPRLAGFLKTLGKEKVLSI
                     LKPY"
BASE COUNT         7168 a         6152 c         6417 g         7602 t
ORIGIN      
        1 atggcttatc cataggctct gtctacgctc tcatggcggt tggatattcg cttgtctatt
       61 cgataatgaa tttttcaaac tttgctcatg gtggagtaat aatgctagga gcctattttg
      121 gattcttttg tcttacgcta ttcaagcttc cttttgcgct ggcattcttg ctcgcttcgt
      181 tgggaaccct tgtagttgcc ataattatag aacggatagc ctataagccg ctgcgtgagc
      241 gaaaagctcc ctttctttac tttattattt cggctatggg cacatccatc atgcttgaaa
      301 accttgtaat tgcgacgatt gggccaactt ttcgcaccta tcccgaagta ttccctaagg
      361 ttccaatcga aattggtaat ctagccattg gaaggctgga tctcataatc tttgtaattt
      421 ccgctgtgag cctcactctc ctaatgattt ttatagacaa gacaaagctg ggcaaggcaa
      481 tacaagccgc ctcatataat atcaaggcct gcgccctaat gggaataaat acagatattg
      541 ttatccttac cgtgtttggc cttggggggt ttcttgcagg catagcgggt gtattttttg
      601 gcatgaaata tacggtttat ccacagattg gcaatattac taccaaatcg ttcatcgcgg
      661 ctgtgtttgg cggtttagga agcctgccgg gcgcggtgct tggctctgta attcttggtg
      721 tgattgagac ttttgttgcc ggatatatct catcgcaatt tcgagaccta atagccttcg
      781 ttattctgat aggcgttctg gttctgagac cgaggggcat aatgggtaag gttactgagg
      841 aaaaggctta gggggtgagc atggatttga catatattca gggaattctg atgctcgcag
      901 gcataaatat gattacggtt ctaggcctaa gtcttttgac gggttttaca gggctattct
      961 cgttcgggca tgctggattc atggccattg gagcctatat tggaacttgg atcagtggct
     1021 ctccagcatc tacgccagcg ggccttgggt tgccttttat cttttccata ttgggtgggg
     1081 gaattgccgc ggggctggtg agctatctga taggcagaat tacccttaag ctcaaaggcg
     1141 attatttctg cattgcgacg cttgggtttg gcgaagcaat ccgccttatt ttcaataatg
     1201 tgaatcgttt tggaggagcc agggggtggc ccgggattcc tggcaggagc acttttcttg
     1261 ccatccttat cgcggacgtc attgcggtgc ttgtcctcgc aaacattgta aattcgaagc
     1321 atggtcggaa tatgattgct gtgcgagaag aggaattggc cgcgcaaacc gcgggcatag
     1381 atgtatttcg ctacaagatg atctcacttg taataagtgc aatatatgct ggagtagcgg
     1441 gagttttttt ggctcattat atgacgttca ttcaacccaa gatgttctct ctcaccaaat
     1501 ctaccgaact cacaatcatt gtcatattcg gaggacttgg ctccatttca ggctcggtct
     1561 taggagcttt ggtgctaacg gcacttcctg agattctgag ggctttcgag atatggcggc
     1621 tcgtattcta tggagcagca gtcattctta ttatggtgag caggccaaag ggtctaatgg
     1681 gtggtatgga gcttacgcca tcagggattc gcaaggcgag tgcagaaaga aacgccctgc
     1741 gcgaggcaaa agcaaagctc cttgttgaag cccaagatgg agaagagcaa tgacaagttc
     1801 tccaattctc catatcaaag aactaacgaa gaactttgag gggctaatcg cagtggataa
     1861 cgttagcttc gatgttcccg aaggaggcat tatagggcta ataggtccca atggcgctgg
     1921 aaagacgact attttcaact taataacggg aatatataag ccaacatctg gctcaatcga
     1981 gtttaagggc aattccttag tgggccttga gccattccga attgcagaaa aaggcataac
     2041 tcggacattt cagaatataa ggcttttcaa gaacttaagt gtattcgaca atgtactgac
     2101 agcctgtcat agaagtgcga attacaattt cgcggaagca gttttccgcc ttcctcgttt
     2161 ccgatatcag gaaaaggagc taaaggaaaa agcagagcag cttttagata ttatggggct
     2221 ccaatgctat aaggacctta ttgcaaacaa tttgccttat gggctgcaac gaaggttgga
     2281 gatcgtaaga gcgcttgcgc ttgacccgac gcttttatta ttggatgaac cagctgcagg
     2341 tatgaacccc gatgaaaccg agcagcttat gagtcttatc ggcaaaatac gagacgattt
     2401 caaactctcg gttcttgtaa tagagcatca catggacctg gttatgggtg tatgtgagaa
     2461 tattgttgtt ctgaatttcg gcgagaaaat cgccgaaggc aatgcggacg aaatatcgaa
     2521 gaatcctctg gtcatcgaag cctatctcgg aaccccggag gaggcagcca atgcttgaga
     2581 taaagaatct gcacgttgcc tatggtggta ttcgagcgct aaaaggcgtt tctctttctg
     2641 tgcaggaagg tgagattgtc acaattattg gagctaatgg cgcggggaag tcgaccttgc
     2701 tgaatacgat atccggattt ttgcgtccgg caaaggggac aattctgtat aagggtaaga
     2761 ctctccctcg gcgcccagac ttgattgtca aggccggtat ctgtcaagtg cctgaagggc
     2821 gcttggtatt tgctaatctg agtgtactcg ataatctcat gcttggagcc tatcgacgga
     2881 acgacaagaa ggctgtccgc aacgatctca aaaaggtctt tgacattttt cccaggctgg
     2941 aagagcgtaa atctcagaga gcagggacac tctctggagg agaacagcag atgcttgcca
     3001 tggggcgtgc cctcatgaca aatggagagt tgattcttat ggatgagcca tccttaggcc
     3061 tggctcctct tctggtccag actgtattcg aaataatcga gcaattccga gggcttggca
     3121 aaactatact tcttgtggaa cagaatgcat tcaaagcact tcaagtcgca gaccgagctt
     3181 atgtgcttga acagggaaga gttgtaaaac aagggctatc ccgcaacctg gctcaggatg
     3241 ctgcagttaa agaagcttac cttggttcgc gtaaacgaaa tggaggaggc taaactcaag
     3301 agcgcaattc ggctcgcttc aggagtagct ctcttaggtc agcttctgta tcgatatcgc
     3361 taagtatatt atagtcatca acctccaggt attcgatatc ggcctgtcta tggccaaaga
     3421 atcctcttag cccattcttt ccattccacc tttctaccag ggagaacaaa gcactatgaa
     3481 ttattggcgg atggcctgag accttttcat atcgaggaat cactattggt ttatccttaa
     3541 tggcgttttt gagcttgacc accgtgctcg gcaatacgaa gggaatatct accggcagaa
     3601 taaaggcgga atcacaattg gaagaaacgg cgcgcaaacc agcgcaaata gaagagaaca
     3661 ttcctttttg gtaatcctta ttgaatatag gccgtattcc gtgcaaacta gcgagtttcc
     3721 ccacattatc cgagtcaaaa cctgtcacta caattatttc ctcaatccct gcttcctgaa
     3781 agcaacgagc ggcatgcaaa agcaagggaa ctccctcgat ttccacaaga ggtttggttg
     3841 ctcccatacg agaagagcat cccgctgcca gaatgatagc aataggttcg tttgtcctaa
     3901 agtcaggcat acagataaaa tcctctagta ccgagcgaat acccaagggt tagtatttga
     3961 gcttgggcag cacacctgat cgatccgcaa ttacttccgc tatgatgctt attgcaattt
     4021 cttccggcgt ctgagctcct atttcaagtc cgattggaga atggactctc gaaagatcag
     4081 tctctgagaa gccttcatct cgaagcgcag caaatatgcc atctcgcttg gttttggaac
     4141 caataaggcc aatgtagcct gctcttgttc gcaaaagaca tgataacatc ttttcatcga
     4201 tatcgggttt tcgagtgagt atcattgcgc aatcctgagg accaatttcg atagccggcg
     4261 cacctttaga tgctgtaaag gggtctgcaa aatcatctat tatcctgagc atattagctt
     4321 ttggaaatcg ttctcttgtg aggagttccg gtctatcatc gcttacaatt actcggaaac
     4381 caaccaggct tgcaacctcg ctcgttgcct tgccaacatg gccggcgccg aagatatgga
     4441 cgaccggatc tggtctaaac ccaagtagga agacctgaaa gatattagat atgccttttt
     4501 caggcaattc taattcgaaa caagaggctc ctgagagagt gcgggcgccg tccaagcaag
     4561 tctgtatttt tttactgctc tcaatgatac tcttggctat cagctcttcg catgagtttt
     4621 gttgatgcgg atataagaca tttcctctct catctattgc agagacatat ttttctccag
     4681 gagtcctcgc aacaaaaaaa gcgcatggat tgccagattc cacttgctca aggcatgatt
     4741 taagggcact ccttgcctta ctctgttgat cgattctttc gagatgcaga gaaactttgc
     4801 ctccgcaggt gatctgattt tgttgcgcct caggcgagag atcaaaaaag agatctctgt
     4861 gatcatagcc agcttctagc atgtttcgta tcgcaataat cgcttggttt tctatatatc
     4921 ctccgccgac cgtaccctcc attttgccat tcatatccac tgccataaga gcatcagtac
     4981 ccctcgccgt cgagccgctt gcgtgagtaa tcgccgaaat aacgactgtc tgatcgctat
     5041 tcaatcgttc cgcaatccag gatcttagtt cgcgcattgt tttaattggc accttcagct
     5101 tttgctctat cgattgccat cagcacgcgc tcaggggtaa aggggtattc tgtcaagcga
     5161 acaccgattg catttcgtat agcattgccg atggcagcag gaggagcatc tattcctatt
     5221 tctccagcgg attttgcgcc gaaaggcccc gatggctcgt agctgtcgac cagttcgact
     5281 gtaatttttc ctacatcttc cctggaagga attgcataag tcaaaaggtt atggctaagc
     5341 atgtggcctc tctccgaata gcgcacatct tcataaaggg caagaccgat gccctgaagt
     5401 gccgcacctt ctatttggat ggtagcaagc ctgggattga ttggagttcc gacatcggct
     5461 acagatacgt agtgtacgac ctcgactttg cctgtttcct tgtccagctc tatctcgaca
     5521 aaacctgcca ggtagggagg aggggatttt gcacctgtaa aggattcggt tgcctctagg
     5581 gttttcattc tttctccatc gtgatagagg gtatcgaagc taaaatcgta gagcgagagc
     5641 gatttgccac cattgagagt cttaaatgtc ttgttatcga ataggacgaa cgcaggatct
     5701 acttcgaatt tctgagcaac cgcttctatg agttttttct tgaggctacg agcagctttg
     5761 attacggctg aacctgagac ataggttgta ctagatgcat aagcacctac atcaaagggt
     5821 gtattatcgg tgtcggaaga gtaaacgatt atgttttcta cgtcgactcc aagttcttca
     5881 gctgcgatct gagcaagaat cgtatcagat ccggtaccaa gatcggtagc gcctatgtgg
     5941 agcttaaaga atcctccatc ctggaattcg attctcgctg agcccatatc gatgagtggc
     6001 aagcctgaac cctgcatggc tattgccatg cccatcgctc gcacttttcc tggagcaaca
     6061 tcccagacca aacgattcgg atcccagcct atgagttcct ttccgcgagc aatgcattcc
     6121 tcgagcttgc aggattcgat cgtcatggcg actccttcgg ttccttcccc catgatgcga
     6181 aatacatcgc tggtttcacc ttcgtgaatc atgtttttgc ggcgaagttc cagagggtcg
     6241 atgcctatct tatgtgcgag ctcatccatg gcggactcga gaccggtgag cccttgaatt
     6301 gctccatagc ctctaaatgc gcctgcggac accttgtttg tatatacgac atggccacca
     6361 aagcgaacgg catttacttt gttgtaaagc ggcaaggtct ttgagccggc aaccatgaag
     6421 actgtcagcg catgctcgcc ataggcaccg gtattagaga gcacttgcat gtcgatagca
     6481 ttgagctttc catcggaatt ggcgccgagc cgcactttga cacgcatctc gtggcgggtg
     6541 aaggttgctt caaagacttc gcggcgcgtg agggcaaggc gagcggatct accggtcttc
     6601 atagtgacca ttgcgacata aggttcgata tgaatgtgct gttttccgcc aaatcctccg
     6661 cctatcctgg gttttataat acgaatctgt cttattggca tctggagggc tagtgcgagc
     6721 aacctgcgcg tatgccaggg gttttgagta gaggtgacga ttacgagcct gccatggata
     6781 tcgatgtaac taaaggccgt atgagattca agtgctacat gttgttgagc ttgagtatgt
     6841 actgtggtct cgacgtagac aggtgagttg gccaattcct gctctacatt tcctatctcc
     6901 atgttatatg cggcagcgat attcctcttg ggttcgaatc ctatcggaaa catctcatga
     6961 atttcgggtt caggatgtat gatgatcgga ttatccagag ctttttcgaa atccaggata
     7021 ggttgtagaa cttcgtattg cactttgact agcattgcgg ctttttctgc cgtaacttcg
     7081 tcctctgccg caaccacggc aacctcatct cccacatagc gcacatattc atcgagaatg
     7141 aatcgatcat gagggctggg ctctgggttg ccctgtccag ctcttgtgat aggtacccga
     7201 gggcaatctt tccatgttaa tacacaggca acgccaggca atgcaagcgc ttccgatggg
     7261 tctatagaag tgattctggc aaaagcatga gggcttctga caagcttgac atagagggca
     7321 ttggctggag caagatcatc tgcaaaggca ggtcgaccaa gcaccaagcc ttccccatca
     7381 atctttgcta tatcgtgagc aacaacattg aatttttgag ttgctttgag cgtttcacta
     7441 tccataggct taatcggatc attattatca atcatggact gcctccagat atttctttat
     7501 cgcacgaatc tgtccttcgt agcctgaaca gcggcataga ttacccgata ggtaatgacg
     7561 gatttctgct tcacttggat ttctgagctc tctcttcatt gataatacgg taagagcgaa
     7621 accaggtgcg cagaatccac attgatctgc cccctccgca gttaaaaatc gagcgaattc
     7681 ctttgcttct tcggcaacgc cctgtattgt ggttatccgc ttgccatcag cgcgcacggc
     7741 tagataggag caagatagta taggcttgtc ctcgatcaga acagtgcaag cgccacaatt
     7801 tgccgtatca cagcctttct ttacttctac atatcctgcc ttacggagta cttccgtcag
     7861 catgtcgcca ggctggcagt ccaggatctt ctcattgcca tttatgtata gggttatggt
     7921 catcctcgca cctccaaaag cgcacgtttg acgagcacag gaaggatttt ttgtcgatag
     7981 ttagcgctgg ctctgatatc gctgccgaac tgtgtctctt tactcgtctc cgcggcagct
     8041 gcttctatct gttcgtcaga gggatttgcg gaatctccca atattttcat tgcatttctg
     8101 caaatgcgag tagctgctgg tcgagaaccg actgcaatgc gccatactgt tcctgtccaa
     8161 gcaacggcgg tattgattat aggaaaatca ctctcggata tcctcatctg ttgataggcg
     8221 gctcttgcgg aagatggcag aattatcttt tctatcaaga aggggctggc ataccctttc
     8281 tcgatgaagc tagaaagatc cttttctaca tttgggtaaa agacaacacg agctccaatt
     8341 gcgcatagtg tggtgttgag atcggagaac ccatagcgcc cagccactgt tcctccgacc
     8401 gttataatgt ttcgtaactg aacgcctaca atatggctta cggtcgttct aaagatcttt
     8461 ccaaatctcg aatcaagcaa ggggcttgtt tctaattccc tataggttgt cattgcgcca
     8521 atttcgatgt ggtcttggaa ttcgcggata tattccaggc ctagatcgca tagatcgaca
     8581 ccgagcatga cagtctttgt actggttcga agccacgcgg aacccccaat cgcagtacca
     8641 tgttcttcgc aaatgagatg catggcttga tccatgctct tggggcgagc atattctcgt
     8701 atttccatct gaagctcctt gataaaaagg atggttcaac tatctcctaa tttttttata
     8761 atagcatagg tttagagaat cagcaaattg ggagtattta tcaacaacct aagcttggct
     8821 atactaaaaa catggcagaa tttgtacact tacacaatca cagcgattat tcccttctcg
     8881 atggtgctgc ttcaatccag aaactcataa ggaaagctaa ggctcttgga atgccaggta
     8941 ttgccataac cgatcatggc aacatgtttg gcgcaatgtc ctttgaaaag gaatgcagag
     9001 attttggcct aaatcctatc atcggctgcg aattctatgt agcaagtggt tctagactcg
     9061 aaaagacggg cacggagaat ggtaataggt attggcatct catcttgata gcagaaaaca
     9121 gtaccggata cagaaatctt ctaaaattga cttcggcttc ttacaccgaa ggcttctatt
     9181 ataaaccgcg gatagatgat gaactaatag ctgccaattc agatggactc attgcttcga
     9241 ccgcgtgtct tgcaggcgag attccgtcgc ttattctttc gggaaagctc gatcaggcta
     9301 taaaaagaat tggcttctat aaagaattat ttgggaaaga tcatttctac ctcgagctcc
     9361 aggatcatgg tctagaagag caaaagatag tcaacagaga gctgataggg ctttcgaaga
     9421 aactcagcct gcctctgctt gctaccaatg atatccatta tgttgatgga gaggatgcta
     9481 tagctcatga tattcttctt tgtattggta caaatcgaaa acgcgacgat ccatctagaa
     9541 tgcattttcc aaacgatcag ttttatttaa aaagtgcgga ggaaatggca tcgatttttg
     9601 ccgaggttcc cgaagccatt gccaatacgc ttgtaataaa cgagatggca aagctcaaga
     9661 tggagtttcc agggcctctt ttgcctaatt atcaagtgcc cgaagaattc tcatctccag
     9721 aagaatatct gcgccatata gctcgacaag gctttcagag tcgttatccc aatccaaatg
     9781 ccgaggccat tcataggctc gaatatgaac tagacataat tatcaaaatg gggtttacgg
     9841 gatatttcct gatagtatgg gattttatca gttgggctaa agagcatggt attccggtag
     9901 gcccaggtcg cggttcagga gctggatcta tcgtagctta tgccatgcgt atcaccgata
     9961 tcgatccatt aaagtatgat ctgctttttg agcgtttcct caatcctgag agagtatcca
    10021 tgcccgattt tgacgtggat tttgacttcg aacggcgcgg cgaggtgatt gactatgtta
    10081 ctcataaata tggcagcgat agagtcgctc agatcattac attcggtaca ctcaaagcca
    10141 aggctgtaat caaggatgta gcccgtgccc tggatattcc ctttgacgag gcgaatcaga
    10201 tagctaagct tgtgcccgaa gatcccaaga tgaatttaca gaaggctatt aagaaggagc
    10261 cgaagcttgc cactcttgca caaaatcctt cttacttcga gctcttctcg atagccgcaa
    10321 agctagaaaa ccttcatagg catagctcga tccatgcagc gggtatcgtg atcggcaaga
    10381 cagatttgat cgactatgtg cctctatata aggatccaaa gactgggctc attgcgaccc
    10441 aatatactat ggaatacctg gaagcttgcg gtcttgtaaa gatggacttt cttggcctta
    10501 agactctgac cttgattcgc aataccatgg aactattgag aaagagaggt atatacctca
    10561 aagaggaaga tataccagaa gatgatccca ggaccttcaa gatgcttggc gaaggcaaaa
    10621 gcacttcgat ttttcaattt gaatcacagg gaatgcagtc tattctcaag cgagccaagc
    10681 cttcgagtat tgaagatctc atagctctca acgcactcta tagaccgggt cctatggaat
    10741 ttatagatca gtatatagat tcaaagaatg gtaaaattgc tattagttat ccccatcctt
    10801 cgcttgaaaa ataccttaaa ggcacatatg gagtaatcgt atatcaagag caggtgatgc
    10861 aagttgcacg agaagtggcg ggatattcgt tgggacgcgc agaccttctt agaagagcca
    10921 tgggcaagaa aaagcccgac atactgcaaa gagaagaagc tccattcatc gaaggtgctc
    10981 ttactcgcgg ctattcaaga gaagaagcaa agcggatttt cgatattcta acgccttttg
    11041 ctggctatgg tttcaataaa tcgcatgctg ctgcttactc agtaattgca tatcgtacgg
    11101 cctacctaaa agcaaattat tctgttgagt ttatggccgc gaacttgaca aacgagatca
    11161 gtaacaccga caagttgacc gtgtacataa gtgaagctcg acagatgggt ttgtctgtgc
    11221 tgccccctga tatcaatcgt tcagaagcca agtttacggt cgataatgga cagatcatat
    11281 atggatttct tggtatcaag ggcgttggcg aggggatcgc aatggagatt caaaaagaaa
    11341 gagagtctca tggcagattt atagattttc ttgattttct tacgcggatg aataatgcgg
    11401 gcataaatcg caaaacgatg gagtcgctca ttatggcagg ctgcttcgat tccttgaaga
    11461 aaggccgcca tgagcttatg ggcaactttg agcgtgctat ggaatatgtg catgagaaat
    11521 cgagttttga agcttcgcgt tcagggatgc tgttccagga ggatgatctg ggaatttatc
    11581 cggattttgt ttttcaacct tttgaagaat tctcgaatca agaaatgttg caggcagaaa
    11641 aggagttgtt gggttttttc ttttctgcgc acccaatgga tgagtaccgg atgatttggg
    11701 agagatcctc gacacttgat cttgggcatc tggatcaggc aagcacaaac cgtgaatata
    11761 tagtggtcgc gcttctcaag gaatttcgca tttatacaac ccagaatgga cggcgaatgg
    11821 cttttggcaa gcttgaggat tacaatggtt ccatagaaat ggtcgtgttt ccagatctgt
    11881 ttgagaaaaa tgaagcgtct tttgtgaagg atcgtgtcct tttccttaaa ggagtactgg
    11941 atgactctcg aagggatttt tcgttaaagg tgcaagctat cctggatgcg gcttctctta
    12001 agaataattc ctggagagaa ttacacatca ggtttaagcg agcagccatc gaagaagcag
    12061 atctctatcc gctaagagat gcgctatatt ctatccatgg gcagtgtcgg gtcatttttc
    12121 atgtgcccat gaaaggagga gcagagactc tgatcgaagc tggagctcat acaaagtgct
    12181 ccgcgagaga tgaggatatg gcgtttatag aaagacaggc tgcagtggcg gaggtatggc
    12241 gcaaatgaag attaagatac ttggcaatgg cggcttctac aatgaaggat tggcctataa
    12301 tgcgctcgct atagatggcc atgtgctcgt ggaaactccg ccagacattc tccaatccct
    12361 tcatagtcag ggaatgaagc cttctcagat agatacggtc tatataagcc ataatcatgg
    12421 agatcactgt ttcggctttc cgttcttctt ttttaattgg ctatatgcag gaggttcgga
    12481 ttctcattat gaaaaacaga cagagctagt tataatcgga cccgaaggac taaaagagca
    12541 tctcaaagct ctcatgcgtc tagctattct gcccgagcat caatatctat tcgattttga
    12601 ccaaagggtc aagtttgtcg agatagacga aagatcgatt gtatccacta gcggtgggct
    12661 atggtttggc tttttgcaca caaagcattt tgtgcccaca ttttcgcttg tggttggtaa
    12721 aactggaccg gataaaggga ttccttcttg taacgactat ctcgagaatg caatatttat
    12781 atatagctca gatacttcca tgttcgatgg catatataag ttattgagct caagcgcaag
    12841 gctcatcctt tgtgatacga atggcgaaga agataatggc gtccatatga gcccaagaga
    12901 acttatggca gccgagaatc aagttaggac aaggagctct gagcgtgcag acctttcaga
    12961 gtataggcta agcggggaaa acgacaagga tcgcgtattg ggaattcatc taagcaagaa
    13021 gatggatagg gcgcaaggac tcaagttcgc ccagcctggc gaagaatact gcatcgagta
    13081 gatgctttta taaagaagct tcaaggattt aatcaatatg cttaaccacc cactccgtca
    13141 gctcttccca attcgaaaag gaaagatctg catctgagtt ttgtcttcca cgcaaggctg
    13201 ctcgcattcc agctttttta gcgcctagga tatcgtactc gacattattc ccaacataga
    13261 ggatctcgga aggctggatg cgtatagtct ggattatacc gtcgaatgca cgctgatggg
    13321 gttttagaac accccaatct tctgtgcaga gcttaaaatc gaataaatca tcaaggccga
    13381 gggatttcaa tttattttgt ggaggcatat cggaaagggc agtgattgtt attcctctct
    13441 gtcgaatcgc ttcgatcgct tgccttgtcc ccttataagg acggatcaat gagaaacgct
    13501 caggcacaag atgatagaag attctttcta tggattgacc aatttgaagc tcgtcttttc
    13561 tgagacgctc cgcaacaagc gatatctgaa aactgtgaag ctcttccatg tttcgaggaa
    13621 tatagttggg ttgtgcttga agaaatctga tttcttttct cacctctgag aatgccatga
    13681 atatgcgggg atggctaaga aacaggtcga tgcatctcag cgcgattgaa tattcgggat
    13741 aaagagtacc atcgacatca aatgccactg cgcgtaggcg ataccgtcct tccataagca
    13801 actttatata tgaagcagag gagggctgtc tattacctat aaagccaata gatcttcgca
    13861 gtggcggcgg ccccgaatat gaaataaaag aatgccttga aaatcttaga ataccctcgc
    13921 cttatagctg gtatgcaata gttcatggga caggtggcca agcggcttgc ctaaaaattc
    13981 cttatatagt gtgactattg cgggattttc gtgagatttt ctaattggaa gtctgcgatc
    14041 ctcttcgtag agcgataggc tacgggctct tttcttttct atatctgcaa gtacaggctg
    14101 tccgcctccg ccaacgcagc ctccggggca tgacatgatt tcaatgaaat gataggggct
    14161 ctttcctgct cggatctcgt ccagcaaaat gcgggcattt gaaaggccat gggcaacagc
    14221 tacgcgaaca ggagagcctc caagcacaac ttcggcactt tttatcgaat cgaagccacg
    14281 cacttggaca aaatccatgt tctgcaaagg tttcttttcg acaatctcat atacagtgcg
    14341 caaggcagct tccattacac cgccagtagt tccaaaaatg gtagccgcac cggtcgattt
    14401 gcccagagga tcatcgaaat cctcttgtgg caaggctgtg atgtctatac ccgcaagcct
    14461 tatcatacgg gcaagctctc tagtggtgag agcccaatcg acatcgaaga atgggcgcaa
    14521 tccggtggtt tccgggtctt tttccttcca ccagtcccat gcggagtcca tttccttgcg
    14581 tctagcttca tatttcttgg ctgtacaggg cattatagag actaccctca tatttttggg
    14641 tgctatattg gcattctgtg cataatatgt cttagcgact gcgccaaaca tttgctgagg
    14701 gcttttgcag gtagagacat gattgagcag atcaggatag aagccttcga taaaatttat
    14761 ccatcctggt gagcaggaag tgatcatggg cagggttccg ccatgagaaa gccgctcaag
    14821 caactcgttt ccttcttcga ttatggtgag atcagccgca aactgtgtat cgaagactct
    14881 tgagaatcca agtctacgaa gagccgcaac catctggccg gttacaaggc ttccagcggg
    14941 aaggccaaga gcttcaccaa gcgatgcgcg aattgccggg gcggtttgga ctaccactga
    15001 aagcctcgaa tctccaagcg cgccatagac atcctgcgat tcgtccttct cggttatggc
    15061 gccggtcggg cataccaagg agcattggcc acattggaca caggcacttt gagctaatcc
    15121 tctatccata aaagttgcga ctctggttcg agtaccgcgc cccgtaaagg caatcgcgct
    15181 tacgccctgg gtatcctcac agaccgagac acatcgtcca cagaggatgc attttctatc
    15241 gtcgcgtacg atgccttcgg aagaattatc gggaggcggg tattttttgt agccaggaaa
    15301 cctatctgca tggactccga gtaaattggc catggtgtga agctcgcaag tgtcggccct
    15361 taaacaggaa aggcattcat ctgggtggtt tgcaagcagt agttcgaccg ctgcttttcg
    15421 tgcttgaata actcgagggc tagatgtccg aatcttcatt ccgggagtag gtttctgcac
    15481 gcaagagcgc aaaagggatt ttgctccttc tacttcgaca acgcatacac cgcaagaggc
    15541 gttttccgaa atatccttga gaaagcagag agtcgggatc ttgacgccgg ctcgagcaca
    15601 ggcttctacg atattctcgt ttgaatcgac ctttatggtc tgtccatcga tcgttatatc
    15661 tatcagattt tccattggtg tacctccttt agtcgccaac caggctgccg cgaggttcac
    15721 gtctatgttc cctcacatcg cagcgaagac agcgatcagc ctcgattcgc gcttgctcgc
    15781 cggtatagcc aagattaatc tccataaaat tgcccttgcg tgcattgatt gggagcattg
    15841 taggtcgagt catcttctgc ttgctagtat tagccggaat ctccattctg tactcaaagc
    15901 atcgaaatag gctgtcgaag cgctttttcc cgcttaatgc ttggtcaatt atctcagcaa
    15961 cgacttttgc ctgtcccatt gcctctgctg ccgttgcagg accgagcatt gcatctccaa
    16021 tggcatatac ttttgggttg gatgttatat gagagaaggg gtcagccttg atgcgcccat
    16081 tcttgagacg ttctacgccc aaaacttcta tgccaggtat ctctactttt tcacctatag
    16141 ccataataac catagagcaa ggaatgtctt cgattttatc tgtaggaata ggtgttggac
    16201 gtcctgaaga atcgataggc cctgccttca tacgctgaat tcggacggct cttgcaacat
    16261 gtttcccttg cttttcctgt gtcatgattt caagcggcga agccatacag ataagctcta
    16321 taccctcttc aagggcaccc ttgagttcat ccaggttagc tggcatatct gccttcgatc
    16381 ttcgatagac gattctaacc gttgccccga ggcgtagagc agagcgagca gcatctattg
    16441 ccgaatttcc tccgcctatt acaactacat cgcctcccgt atgaaccttt tcttttttcg
    16501 caagcgcatt gagaaaatca caggctttgt agacaccgat agcgtcttct cccggtatat
    16561 tcatggtaag ttcggatgaa gcaccagcag ctactattat ggcatcgttc tcttcataga
    16621 gtttcttcca ctgttccggt tcggttatgc gtgtattgaa tacaaagcga gttcccaact
    16681 tttctatgag actgatctct ttttttagta tatcctttgg caatctatat gatggaatac
    16741 cccagcgaag cacacctcct gcctcttgtc tagattcata gatggtcact tcatgaccaa
    16801 ggcgaaccaa gtagaaagcg gccgtcaagc cagcgggtcc tgctcctatt atggcaattt
    16861 tcttgccgct agggggcaat ttttctttga gaagcttcgt atatacttgt ttttctctgc
    16921 ccatcttata catggtgtcg gcaagatagc ggtgaatctc tccctgtgat acaggttcgt
    16981 ctagcatgtc acggcgacag cgcatccggc aatgaaagtg gcagatacgt ccaattgtgc
    17041 cgggcaaagg attttcccgc agagtgagct caaaagcatc ttctatacgg ttttccttta
    17101 ggagctgcaa gtatcctgga atattcatgt gcaaggggca ggaggattcg caaagagcaa
    17161 tgtataggtt ttcgcaaacg ccagcagggc agcgtttccc ttttatgtgt tctatatatt
    17221 catctctaaa atagcgcaag gttgtaagaa cagggtttgc cgaggtttgc ccaagaccgc
    17281 agagagaaga gtccttgact attattgcga gttcctcgag cgtgtcgaga tcttcctcac
    17341 tcgcttcacc atcggatacc ttttcgagaa tatgcagcat ttgggaagta ccttctctgc
    17401 atgatgtgca ttttccgcaa gactctcccg atacaaagcc tgtaaaatat ctggctacat
    17461 cgaccataca attatcttga tccatcacca ccatgccacc cgatcccata attgccccga
    17521 gctcattaag gtgttcgtaa tcgataggag tcttgaatag gctcgctggc acacaacctc
    17581 cggatggtcc accgcattgt acagccttga tttttctctt cgggcccatg cctccacaaa
    17641 taccatagat tacgctttca agtgtgctgc caagaggaag ttcgacaagg cctgtattcc
    17701 gaacttttcc gacaaaggaa aacacctttg ttcccgtact cataggagta ccaaaacccg
    17761 caaaccactc tcctcctctg gctgttatca ctggtatgtt gcaccaagtt tcaacattgt
    17821 ttatcgaggt tggacgacca taaagacctt tctccactgg gaatggaggt cttggtcttg
    17881 gtcttccagc attgccttca atagaggcta tgagtgcggt ctcttcacca catacaaaag
    17941 ctccagctcc ggtcactaaa tctatatcga acgagaattt ggatccaagt atgttctttc
    18001 caagaagtcc atgctgcctt gccgtttcta tggccaaatt gagcctatag acagctaatg
    18061 gatattccgc acggacatag acaattcctt tgcttacatt cattgcatag gcgccaagta
    18121 gcatcccttc caacaacata tgaggatcgg attcgatctc atttctgttc atgtaagcac
    18181 caggatctcc ttcatctgca ttacagatca aataggcttc cccaggatta tcgataagcg
    18241 atttacgcat gagcgaccat tttttccagg ttggaaagcc tgcaccgcct cgtccgcgca
    18301 gcccggattt tttcacttct tcgatgatag cctctggtgg catggaggtc agagctcgaa
    18361 ccagggctcc gtatcctcct acggcgatgt attcttcgat gcattccggg tcgatcattc
    18421 cggcatcgcg tagaatgatc ttttcttgat ccctgaagaa gttgatatct ttccatgttg
    18481 ggaggaaatc ataaccttcg ccaaaatcca ccttagatgt cctgaagtcc cattcttcga
    18541 tcttagcttc tgccattttt gcaatctttt catatccgag atcggatgaa agaccagcaa
    18601 aaatacgcga tgctttggat gtattgactt ctgtaaagaa aagaaccggc tttcccggtc
    18661 tccatgtcat tacaacgggt tcagctgcac aaaaaccaaa gcatccaaca cgacgcagac
    18721 gaatattggt attctctgtc ttctgagaaa gagtctccca gaccttatcg gctccagagc
    18781 ctatgccaca ggttcccatt cccacagaaa tccaaggagt atcgccatga aaatggctaa
    18841 agccttcctg tgctagaaat cccaaatcgg tgttcttgtt catcgcccac ccccccttct
    18901 gatcttctcc agaagagaga cgagcttact gacagtcatt cgcgatagca caacaccatc
    18961 gaccattatg acgggtgcaa gagcgcattg gccaaagcac gctaccgtgc gaatcgtgat
    19021 tagattatcg ctgctcgtca ccgaattttc ttctttttca ttgaattctg ctattctaag
    19081 tctggagaga gcttcgttta ggagcacaag ggagcctctt gtatggcatg ctgtaccgcg
    19141 gcagacaata atgacatgct cgccttgcgg tttcaaactg aagaaagaat aaaatgttgc
    19201 cactgaatat actctagata gaggtatatc gagtcttttg gagatttccc ttagtgtcgt
    19261 ctcggagaga tattttttgg ggtcattttg ttgtgcctgt tccaaggctc caagcaggcc
    19321 tccatttttg cctttgtatg aatcgatgat gctttcaaca ttcaaggatt ccgaattgga
    19381 gattgttttt gtcatgctac ctcctttgac atcttttatt attctatatc gattaggata
    19441 tttgtcaaga aaaatttatc tataaaatca taattataga acaggcaaat tcgaatcata
    19501 atcattgtat ctcaataaat agtaaaatat cgataaaaag agtattgcat ccagtctggt
    19561 tttatacaat agtatagata tggctctgaa taaccgagaa gatgaaggga tttctattgt
    19621 tcgtcccaaa tggcccgcta aattcgccaa ccagagtgta gattgtttgg gtcgaaaatt
    19681 gaaacgcatc ttcgatcaga atgccagttc ggactacttt accagcgatg aaatcagaga
    19741 ctattcctac tacgaaaggc tgctcataca agagcttgaa ggcctagacc aaaaaatatc
    19801 aaaaggaagg ctaaagccaa gctatattgc ggaatatcca agggttattc acgccgatga
    19861 aggcaagatt cgcgcttgta tttatattgg ttcatttgat ccttttcagc ttacccatct
    19921 caccgttgct ctgcgttttc ttgcctctga tctaagcaac tcagatttcg tagttatagt
    19981 acccgaaggt tcgcctgata cagcaaagcc tcttaaaacc gattatgctt ttcgctattc
    20041 gatagcgaag atgcagattg agggtatttt cgactccttt attaaggcat tagatctagg
    20101 catgcaggct gatacaatcg aaataataag aagatttata gcaatgcaca gtggatacac
    20161 tctcgaattg acacatttaa taggttcaga tgcgcttccg atagctgcaa gtctgatttc
    20221 ggaagatatg cggatatgga gaaaagaagc tcagcaatca aaagtagact atcagcattc
    20281 tatatatgtt gtgcaacgag gagatatacc ggaaatgcac tcctacttaa aggccttgaa
    20341 agaggaagac atccagatca tcatcgatcc tttcagtgta gaggctccct cgagcagcga
    20401 ttttcgaacc aaacaagcct atacgattgt attgccgact gcttccatta gagaaaggat
    20461 ggagataatc tttcgttatc atttgcacaa aagttggtct tcggatcgac aataatatcg
    20521 aagaaattgc ctgatttgga tgcagatgaa aaaaaggcac gacaagctct tttttcgagc
    20581 tttgaagtgt tgtataagta ttttatgaat attcagtcaa tgaaattgat ttttctcgga
    20641 cctcctggag caggcaaagg gacaattgcc agaatggcct cagagaggct gggtattcct
    20701 cagataagca caggtgatat atttcggaca gcggtaaaag aaaagactga acttgggctg
    20761 aaggtccaat cgatacttgc ctctggcgct ctggtacccg atgagttgac aatcgccata
    20821 gtaaaagaaa ggctatcttg gccggatgta aggcaaagct atatcttgga tggcttccca
    20881 aggacgattc ctcaagcaga agcgctagcc tccttttcaa aaccggattc agtagtggat
    20941 tttgaagtag atgacggttt gatcctcttt cggcttaccg gaagacgagt atgtaggacc
    21001 tgtggagcca tctatcatat agttacaaga ccttcggcaa ctgaagggat ttgcgatctg
    21061 tgcggaggtg aactatatac gaggaacgat gatagggaag aaactgtcaa aattcgactc
    21121 gaggcatatc acaaggaatc cgaagccttg atagaatatt attctagcca gggaattcta
    21181 atggttattg atggcagccc tgatcccgag actgtctatg atacttttat ttcaaagatt
    21241 gggcgattct tgaaaaaatg attatttcgg gatctcggtt ccggaagaca gcccgatttt
    21301 ttccgagcct ttggtttttg ttttttatct tgactatttt ctcaatcttg cctgtcaaaa
    21361 gtgcaggaac agaggagatt gtgccagggg tatggaaagg tgcgctagta ttgcgagaag
    21421 ttgcacaaga gcagtcttcg ccaaaacttc cttcttatcc gaaggatcaa caatcgatgt
    21481 ctttaagttt tgagttttcc ttgaagattc tctctcagga tagaggatta ttggtcgata
    21541 ttcctgaaca aggcttgtat tcttacccta tagatgaata tagcttcgat actcaacaga
    21601 tcagctttat ttttgacgcg atagaaggag atgaagagct actctttgtt ggcacttact
    21661 cctcatcttt tttgcctcct ctgagtaatc atagaggagc catagttggc tcggtcagag
    21721 ggcgatcttg gaatgggtca ttctatgtac aaaggcaaga ttcgtcagtg gatccgggtt
    21781 cgattttttt ggatatcccc atagaggatg gagcacttcc cgccactcta gtctttcctt
    21841 tgagcgagac cccttctctc gatcctaatt cttcatctga ttttcctctc atattgctag
    21901 tagctggtgc tggcagggca gatagggatg ggaataatgt ggatgtacct gggaaaaacg
    21961 attccctgaa acaattgtca aatatgttga gaatgcgcaa tgttggaact cttcgctatg
    22021 ataagcgagg tgcaggcgaa gcatacaagc tcgaaaagcc aggtcgcatg accagctttt
    22081 ctgagcacgt cagagatctt agctctgtaa tcgtcgctgc cgcagctata cccggacatg
    22141 gaaggctgat agtagctggc atgaacgagg gagcatggat tgccatggca gctttgaacg
    22201 acgaaaggct tgcctcactt gtcgatggtc ttgccgtgat cgatgccagc ggtcagagtc
    22261 cgatagagac cttgaaacag tctcttgcag gcttagatga agatcttcga aaaaaagcat
    22321 tggatgcagc gcagagcctg gtccaaaatg gagttttgat cgatgtccca gcagagcttg
    22381 cagatttttt cgcaccgagc agaaaagact ggctggcaac ttggcttgcc tttgatcctg
    22441 tagagacaat cgcaaaagtc cgaattccaa ttctgctcat ttatggagaa aacgatcttc
    22501 aagtcagttg gcaggagttt tccatgcttg tcgaagcaag accggaggct gccattcgaa
    22561 ttgtgccgcg gatgaactat atgctaaaag aagtcaagag cgaggacgaa aactactctg
    22621 ctttcactga tccatctttt agggtacctt cccttcttgc agacctgctt gcttcttttg
    22681 caaaagctca gcccgccccg cagggtttta tactctggca ctaattatcg aaagtcgaga
    22741 tagaaagacc aacatctacg agttgccgtg cgctgatcat gacaggctgg ctaatgaaga
    22801 tcgatccttg ctgttcaatc gcaatctctg gagaaatggc aataccataa gagcttagtt
    22861 tagaagacag cataacccaa agaaaacgaa cgataggctt atatgtgcgt gcactttgtt
    22921 catccttcat ccgtatctgg aaagtggtgc gatacatagc agaagggtcg cctttaggag
    22981 gacttgtttt ttcccggatt gcactcatca aaatgccctc gaaaggcaaa gtgacccctt
    23041 ccataatagg taactgactg acaattgtag atttagcatc gatatatatc gcaaaatcca
    23101 tagcatttgc gagaactccg ctcctggcag ccagtgcgct tgctccggga ttagtgatag
    23161 gggatgcagg gggcacccat gttgcaagat gaacttggcc ttggtttgga ttgcggacat
    23221 atagacttcc tgattttaga gcatagccac cattgatctt cttccagttg gaatcaaggc
    23281 taaaagacag ggaagttaag agatcaggaa aatttcctgc aaataccgct tctaaagctg
    23341 gttggcctgt ttcggtgagc gaaactccta ttcctacaac ttgcgctcta gtcaatagct
    23401 tgtcgatgtt agcatgatcg attggttttg aaggttcttt ctttttttct gattcatttg
    23461 atttatcaga agcaatagtt tcaatagctt ggattgttga atcatctaag catgcagcta
    23521 tatcgcgaag gaaaatatct cctgaacgta aaataacatg cggcgttttt tcaaaacgat
    23581 cgaatatccc tgcaatggct atctccttag gcacggagga gcaggagaca acagaaatac
    23641 agattgctgc taatagaata atgtcagcaa tcgaggtatt ttttattctg ctttcttttc
    23701 tattatttgg tagaaatcgc atcaaggcca aattcctttc tttttctttg agtaagctaa
    23761 gcagaatata tcacagaatt ctcaaacata tcgagtaagg agattgatgg gagattgatg
    23821 gaaaactata tcgcgctctt tatgatagga aaccagaatc aattgagcta tatgcgtagc
    23881 cctgtgatga cattatgatt caaagaatgc tattttgaat gttgaatcta tatcttatct
    23941 ggcaaaatag catatggagg tatgcgaaat ggctgctgag cgaacatttg cgatgctcaa
    24001 gcccggtgtc ttatcccggc gtgttgttgg agagataatc tcgaagatcg aacgaaaggg
    24061 cttcaaaatc ataggtttga aaatgatgaa tgtcacaatc gagcttgccg aaagacaata
    24121 tgccgaacat aaagggaaat ctttttattt agaccttctg gagtatattg tgtcaggacc
    24181 tgttgtggta atggttcttg aggcggatca ggcaattaag cttttcaggc ttttgtgtgg
    24241 ctttacaagt ccagaacaag ctctccctgg taccatcaga ggggattatg ccatgcacac
    24301 tagtatcaat ataatccatg cttccgattc gaaggaatcc gctgttcggg aaattgatct
    24361 tttctttaga cctgaagagc tcatagattg ggaagacggg aatcaaaaat ggatctaggg
    24421 tgagagcctc atttatgagg aggattaatt cttgaatatg ttattaccag aaaatgcaca
    24481 tatcaaagga ggaggtctta tgacaagcac agttgaagta ccgtgctgga atcttgacga
    24541 tgttttccgt ggctttgaat ccgaagaata ccaaaaggca aaaaacgagc tcaagagcct
    24601 tttggaaacg ctgaaaagag atcttaccga tgctaagccg ccttccgatg caaaagggac
    24661 ggctaactgg ctattcgagc ttctaagcag gatagatcgg ctcgagacgc ttgcagatac
    24721 gctctctgcc tatgtccagg cgcgttttac caccgacaca agggattcgc ttgtgattca
    24781 agagctcgga aaagtggagg agcttctagt tcctgccaca acgatcctcg tgctcttccg
    24841 caatatgcta gctcgttatg aaaagagaat taagaaagcc atatcacatg atgccagatt
    24901 cgtagactac gcttttgtac ttgaagaggc actgatgttc caaaagcatc agatgttacc
    24961 tcaacttgaa gacctggctg cggatcttgg tcgctcagga gccgatgcat ggtctcggct
    25021 tcaggattcg atccttgcaa atgccagtgc cgtgtggaat gaagcaacag gagaacgcaa
    25081 gacaatggta gagctcagaa atctcgccta tgatccggat cgcaacattc gcgagaaagc
    25141 ctatcggctc gagctttcca tatggaaaac atatgaaata ccggtagcag ccgctctcaa
    25201 tggtgttaag ggcactgtgt tgactttgaa taagaggaga gcctgggaga gcgctatcga
    25261 tgcttctctt gctcaagcca ggatatcgat ggcgacactt gaggccttga tagctaccat
    25321 ggaggagtct cttcctatgt ggcggcgtta tttgtgcgcc aaggcgcggc ttttgggtct
    25381 tgatcgactt tcatttttcg atctctttgc acccctacag aaccaaggag ccgtgattca
    25441 gagctatacc tggtcagatg caaaagagtt catcgtcaga caatttggca cttttgataa
    25501 agaaatggcc agctttgccc aaagagcctt cgaaaacaga tggatcgatg cgcttccccg
    25561 tgaggggaag gtgggcggtg catattgcac acacttccct gctgccaaac aagccagagt
    25621 cctctgcaat ttcgatggct cttactcttc tcttataacc atagcacatg aactaggcca
    25681 tgcatggcat tacgagaata taaaggattt gccaagcttg ctttctcagt atcccatgac
    25741 gctagcagag actgcttcca tattttctga gaccctggtt tccaatgccg ccatggctac
    25801 tatgggagag gatgccagag tgcctctgat cgagcttcat ctacaggatg cttgccaggt
    25861 gattgtagat atcctttctc gttattattt tgagcatgat gtatttaaag agcgagcaaa
    25921 aggcgagata acgccagatc gcttaagcga tctaatgctt caatctcaag cgaaaactta
    25981 tggagagcct ctgcgagaag atgaacgtca tccttatatg tgggcggtca aaggccatta
    26041 ttatattccg gggctttctt tctataactt tccatacgct tttggtcaac tcttcggaat
    26101 ggggctctat agtatctata aagaagaggg ccaatccttc gcggccaaat accgtgagct
    26161 tttgcggttt acgggctctc tacctgccgc agaagtggca aggcgcgcag gtttcgatat
    26221 cgaaagaccc gaattctggc agggcgccat gaagacattt gaacacgagg tggaatttct
    26281 tgaggctcag agcaacacta gatagcctgt agatccctta tcaatagggc tttagaatcg
    26341 ataggacttt ctcttttccc agtgttttaa ggaagcctgc cagcctcggc ccttgatcct
    26401 tacctatgag agcctgataa gctgcaatga atagtctttt aggttctata ttgctggatt
    26461 gagccgcagc atagattgcc tcggcaatat cgttctcggt agcataggta tcgagctttt
    26521 ctaccacctc gtcacgcagg cgtcgcaatg cggctgcctc ggcttcgggg agagaggcga
    26581 gttcacctgg cttacgcagg aagaacctga agtcctctgg tgcgcattcg gttatccaat
    26641 accaggcaca ttgagctctt cgctctacgt gcgctttttg cgaaggcttt aatcccggaa
    26701 gaagcgataa cacggcatcg atgtttccca ggtgaatctg caatagattg catagatgcc
    26761 gaaatggtat ttgatagggc atctcctcag gaaccctatc tacctgagac agttcataga
    26821 tgcggcgttc gagagcttcg acttcttcgt tctttgcttt ttcgacgccc caatagatgc
    26881 gctcagttct atcgtaatcc tcgtagattt taatcacatc gagatcaaaa ctgatcacaa
    26941 actctgtatt cgggcgagtg ccagcaaaga gatagcgaac tatttctggt tggtagactc
    27001 tcaacaagtc ctgaagatcg acaaccttgc cctttgatga ggacattttg ccgggcgagc
    27061 ctttgatacc tataaaatca tagcggaagg tcactggtgg cttcctgcca taaacctctt
    27121 cgaccacatg cttcgaggta tcgaaagaac ctccttgaga atgatggtcc ttgcctgcag
    27181 gctcaaagtc gaccttttca taagcccaac gcataggcca atcgactcgc cataggagct
    27241 ttgctccctt gagcgtacgt aggtcaccct tttctagaaa accgcaggtt tcgcaatggt
    27301 aactcaagcc ccattcgcca tcccaatcat cgatctcgg
//
LOCUS       DAOMZL010000017        27008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_1450_length_27008_cov_20.759210, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000017 DAOMZL010000000
VERSION     DAOMZL010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 27008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27008
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1450_length_27008_cov_20.759210"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLQ30_02635"
     CDS             <1..282
                     /locus_tag="PLQ30_02635"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPW01370.1"
                     /translation="VAQVISNVIDFGFPIQEAILAPRLFQAASGSLNLEGRYPVSALD
                     GIKKLGHDVTIRGDWDSYFGGVHAVLYDYNEGLLYGGADPRRDGQAAAF"
     gene            483..1064
                     /locus_tag="PLQ30_02640"
     CDS             483..1064
                     /locus_tag="PLQ30_02640"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HPW01371.1"
                     /translation="MKNARRIIPILLVPLLFFGGGAILPLAAYNLKTAFAPQPALLTS
                     IGQSADVEMAKTIMARAKLNFTMDPTIKAQALASTNAKTLVIVIGGSSKGLGAAGISA
                     EAELERTKALLAEAKKRGMKIIGLHIGGEARRGELSDRFINEAVPSCDYFIVVEEGNK
                     DGLFTKLCGNKIPLDIVQKISQIGEPLAAAFLK"
     gene            1075..2346
                     /locus_tag="PLQ30_02645"
     CDS             1075..2346
                     /locus_tag="PLQ30_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPW01372.1"
                     /translation="MTQELWLFILMVGAFASGCFLFKWPVSVAMLVASALAAIVGGVQ
                     DPIRHLVEGTFGYLDTILTIATAMMFMTGFRDSGALQALTAAVIKKFNKQPALLMILL
                     TLVIMVPGMITGSSTASVLTAGAVVAPMFIAMGMSTIDAGALISIAAILGMIAPPVNI
                     PVMIIGGGVDMPYVGFEIPLILLTFPLAIVFALWFGWRNVRKVDRSHVLASLDFTTLN
                     KYGALRLFSPLIFIFVLMILDKTVPHIFGFGMPLIFMLGTVLTWVTGTRFNILKSAKK
                     SIGDVLPVLGILVGVGMLIQVMTATGVRGFIVVNALSVPSSLLYLMIAISIPLFGAIS
                     AFGSASVLGVPFMLALLGHDQITTAAALSLIAAIGDFMPPTALAAIFAAKVVGVEKYG
                     SILKKLVVPALIIIAWALFFILFSKQISALY"
     gene            2369..2512
                     /locus_tag="PLQ30_02650"
     CDS             2369..2512
                     /locus_tag="PLQ30_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01373.1"
                     /translation="MFYIYLGILAIVLALTVWELFDTKKLSMQITAAMVIMPLLLRLL
                     MIK"
     gene            2526..3653
                     /locus_tag="PLQ30_02655"
     CDS             2526..3653
                     /locus_tag="PLQ30_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HPW01374.1"
                     /translation="MQKHTITAIICLAGAIVASAIAGSSFLAMRKPDKIVRGPGVTEI
                     KMLSEWFPDLKGSPGDTEVYIMEGADNGVSMLVLGGTHPNEPASHLAAILLIENFLPR
                     TGTLYVIPRANASGFTANDPQEGAPQHFTIETPNGLRVFSYGSRATNPIHQWPDPNIY
                     IHASSGQTLSGSETRNLNRGYPGRADGTLTEKVCYGIVQLIKEQKVTITVDLHEASPE
                     YPVINAIVAHPKAMQLASDTVLGLEMQDIKISLEPSPVNLRGLTHRELGDFTDTLPLL
                     METANASQGRLRGRTDEALVLTGKDKWYVRAAKLGRLYVPYDENGHPIEERVGRHVTA
                     IAEIINSWNWEHPETPLEFDNIPSYEDLLSKGLGEYLLPVQ"
     gene            complement(3935..4915)
                     /locus_tag="PLQ30_02660"
     CDS             complement(3935..4915)
                     /locus_tag="PLQ30_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPW01375.1"
                     /translation="MIYRHLGSAGIKVSVLSLGSWVTYGSQIDIDSASQMIKYAYDHG
                     VNYFDNAESYAGGQSEVIMGQAFRRLGLRRGSYLVSTKLYWGLHEGPNEKNTLNRKYL
                     LEAIEGSLERLGMKYVDLLFCHRPDPETPIEETVWAMHDIISSGKALYWGTSEWSAEQ
                     IRQAWETADKHNLRKPQMEQPQYNMLVRTKVEKEFARLYQGIGLGLTTFSPLASGLLS
                     GKYKEGIPKDSRFALYGYEWLQERWYQPEIISSIERLRPIAESIGSTMSQLAIAWAAR
                     NPNVSTVITGASRLSQLEENLGAIEILPRLNAEILEKIDEALEPILVMSN"
     gene            5145..6419
                     /locus_tag="PLQ30_02665"
     CDS             5145..6419
                     /locus_tag="PLQ30_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HPW01376.1"
                     /translation="MSNSDRIFKIVHISDLHLGKTLRSQDLAQDQSSILKQIVEKVKM
                     LKPDLVIIAGDVFDRSIPSESAQTMFGWFMAELRRALPKDGRILVIPGNHDSPKRIAF
                     AAELFESVGIHIVSEILSNPTLVLKKDGKRAAVWALPFVTHGAYHEFKRNLTKTGYAE
                     MASAEANSSMAGRMESIIANLRPSKSASESEYDLNILVAHCYVKEAVLSESDIAFIGG
                     TEAVPASLFESFDYVALGHLHKMQALSPKVWYSGAPMSMSFGDGDIEKGFLYVELEDG
                     KPARVQRMELKPLHVFKRVRGSFDELAAESALIDDDYVEVILTDEEPVYDAFNRLAIR
                     FPNLMTVRQEAFEKRIGTEVEEVGIPVPHIVSDGETENTDALEDFSGKSLHATVLKDF
                     VGFTQRILDEPPSEELLNAFEVLLRNAEEDLL"
     gene            6416..9964
                     /locus_tag="PLQ30_02670"
     CDS             6416..9964
                     /locus_tag="PLQ30_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW01377.1"
                     /translation="MKPLYLSLENFGPYRQRQDIDFCKLDDFFLIYGKTGSGKTTIFD
                     AITYALYGKAIGGRSNLERELGSRFSPIGSKPWVEFEFLASSAQWKVYRSLPYKKRNK
                     RGVESEATSEVELYRMNEIGVYEIIADRSTTANQILLDLLRLSADEFSKIVLLPQGEF
                     QKFLEMKTTERVEILEKLFDVGIYDAVTEAARKKVMELEAYLKAKQEEIERISNELGI
                     EPGSRIKESGKAISEFDKRIEESTKDASQLETQIAQNRERLSFLQSMLAAWKNLHEKE
                     ELKPVFDKRETRLEAAKSLGELASLAKQVSADYEELIELLSESLQTGTELSKLEAEGI
                     EIKKIQKGLPIVRKKYDNLQRSVALYQNAVNAWNQKEQCESERRQVMQRLDATLQDWE
                     YRQNEIGQLKSSITEIENAIQSEPLAQESFHKIVIADEAFKRIETWIEQKEKIEAQRV
                     ETLRKIREAEDSILLTNQELTIAERNRDRINEEIRKAQAGMLAASLIPGEPCPVCGAR
                     DHPNLAKLEQNAPDDKTIKTAQKAVEETKSSLSAIIQDKQNLENRIEEFSQDLQVIVQ
                     KISEEWKGIACEVIGDKSTMQLLDISPSEFVSISSRLQSERRELEERLKSYQHGRKSL
                     QELRKTLDQAGRDLDRIQEEKTRLEMKQTELDAHIDSLGEQIGKEDPRICLKEAEENL
                     VTLHNEIEQIEEQAQKWEIAWSSISIKMDMQLEKIGQKGPALATMVEEFLNEVDKMKV
                     RSLLRLLESPVTSSEKEAPEHEQQAIRPLPDLLTSSGNNAGNLSGFSESIYEPSPRLL
                     QELFALLANSLPHIDRFVPVSGQIMYLSETLEKILSAHRINEPALKALMQEILCSVWT
                     SNRIKEEAREISAFRQSYNEASISYQVLAARAQALSPFNSLSPNSFNSTTLPPSNQSL
                     DRLPQHNQENGDQAKHQEMFDGIHLEQQEKNLREECLSLLERKKSIETEISDLRDERA
                     RFIAELDQLRRLLSRRDELTSEYERAIKEFGKHQELSKLLGGSLNPQRKMPFKNYVLG
                     LQFREIAMRASSRLYRMSNGRFLVEADILGGSGNQKIGLELYVLDAWNGGRRPVGTLS
                     GGEKFMLSISLALGLADSIQERARANRIESLFIDEGFGSLDEESLSLAISVLEDLRGD
                     KSIAIISHVDELYSRIPSRIVVKKSVGGSHLELEQD"
     gene            complement(9977..11149)
                     /locus_tag="PLQ30_02675"
     CDS             complement(9977..11149)
                     /locus_tag="PLQ30_02675"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPW01378.1"
                     /translation="MYEFYAPTKVLFGDGCLAEASPRLKEMRATNVLIVSGRSKTASL
                     QGFRELCIGLDASGIRYVHFGEVSADPDTSLVDKGTEVYRAQHCDAIIGFGGGSPIDC
                     AKGIAASVAEGRSIRDFVGTGLAFTKPVPPLIAIPTTAGTGTEVTNAAVFTVVDESGR
                     RSKKGTSSQFYFPKLAIVDPVLHLSMPPSLTAATGMDALTHAIEAYVSRFNTPISDMY
                     CLESIRLIGRSLRSACHISEGAKAMQSRRDMALSATLAGVALSEAGLGMVHGFAHAVG
                     ALAGIPHGLANAIMLPFVMQSLIPDAKGRLSDIGQALTAKDNISPEESVRAICQLGHD
                     IGIPENLEAAGVSPSYFEDILSDALSYRRRKASPRAFTDEELRELLERAFHGAPQQ"
     gene            11394..12944
                     /locus_tag="PLQ30_02680"
     CDS             11394..12944
                     /locus_tag="PLQ30_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HPW01379.1"
                     /translation="MKKILFALIALVAVLGLSAQDFTIVNGTEPTSLDPHAVEGVPEH
                     RIYVALFEGLTINDPRTNRALPAVAESWSFSKDYKTCTFKIRKGVTWSDGVEITADTV
                     VQSWVRKMDPQNAFQYADLPAMYIEGGMDYLEGKAGPESLKIRAVDKYTFEVKLVGPI
                     PFFADMVSHYAFAIVPMHAIEKYGKDWVKPGKMVSNGPYVLSEWKPQEKVVVVKNNKY
                     WDAKNVSLKKVTFIANDDINVGYNLYKSGAADWTEAVPLELMDEVKLRKDFHVAPQYG
                     TYYFIYNTTRKPLDDARVRKALAMAINKDDLVNKVTRGGQIPANSLVPPSTGYTPAKG
                     YNFDPAAARKLLAEAGYPDGKNFPAFTILYNTSSSHKSIAEFIQAQWKVNLGIDVSLM
                     NQEWGTYLDTRSQSHNFDIARAGWIGDYLDPSTFLEMFITGGTQNDGLYTNPKYDELL
                     DKARVASGTERFQYLMDAEKILIDEDMAVLPLYFYVTQNLIDLDKWDGWYANPLNIHP
                     WKFIRPKK"
     gene            13171..14097
                     /locus_tag="PLQ30_02685"
     CDS             13171..14097
                     /locus_tag="PLQ30_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW01380.1"
                     /translation="MPRYFIRRILSIIPTLFIVITLSFFLIRLAPGGPFAREREVPEA
                     ILQNLMKRYHMDEPLIKQYLRYMGDVIRWDFGPSYRYRDLSVNEIIDEGLPISMSLGV
                     ISLVLATVGGIAIGIISALKQNRWQDYVVTSIAVIGISVPLFVMGPVLQLVFGMKLKI
                     LPIGQWISTHGIKAVILPAITLSFPYFAYIARLSRASILEVLRSDYIRTARAKGLKES
                     VVVWKHVLKGAMLPVVTYLGPAFSGIVVGSIVVESVFLVPGIGRPFVQSALNRDYTLI
                     MAEVVVYSIILIIANLVVDILYGFLDPRISYK"
     gene            14108..15346
                     /locus_tag="PLQ30_02690"
     CDS             14108..15346
                     /locus_tag="PLQ30_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW01381.1"
                     /translation="MAKKQLETAVNEFNQVMKPVSLWADAWKRLRKNRMALISLVIVC
                     IYLVISIFAPLLTSAGILIDYRKQVITNASLPPSFKPAGELVINKIEKRIADLEQRVE
                     EQKQQQSAFDSFFDFGSVYAQDENGEAGDDFAPSQEEFSFTYEGEGFFGNTTDPVVRE
                     LISQKETLERVKAELDTNPDYKIVYILGTDNLGRDMFARIIYGGRISIAIGVVGTLTA
                     ALVGILVGAISGYVGGWLDNFLMRFVDIMYGLPYMLIVIIIMAMIGEKARGSFVVLFV
                     AIALVSWLTIARVVRGQIISLKNSEFVEAARSMGASTQRIIFRHLLPNTLGVIIVFST
                     LMMPSFIMNESFLSFLGLGVSAPDASWGTLVSEGVRAMESYAWQLLGPGITMTIFLFC
                     MNFLGDGLRDALDPQSKNRT"
     gene            15359..16351
                     /locus_tag="PLQ30_02695"
     CDS             15359..16351
                     /locus_tag="PLQ30_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW01382.1"
                     /translation="MSDEVILQVRDLKTYFTVDEGIVKAVDGVSFELHRSETLGIVGE
                     SGSGKSVTNLSVINLIPTPPGKIVGGEVLFHGTDLLKLPPGEIRHIRGNKISMIFQDP
                     MTSLNPFLRISTQMIETIQLHQGLDKKAAKEKAIEMLRLAGIPAPEKRIDQYPHQFSG
                     GMRQRVMIAMALSCNPEILIADEPTSALDVTIQAQILELMKELTRKLGTAVILITHSL
                     GVVAGMCDTICVMYAGRIVERGRTEDIFENPKHPYTIGLIRSVPRLDQETKMRLYSIP
                     GQPPNVIDLPDCCPFYPRCEQSMDICRGKYPSIVSFENNQSVACWLYNKETQNA"
     gene            16344..17351
                     /locus_tag="PLQ30_02700"
     CDS             16344..17351
                     /locus_tag="PLQ30_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW01383.1"
                     /translation="MPETDEVLLDVRGLKMHFPVKTSMFSKTKNYIYAVDGIDFKVRK
                     GETLGLVGESGCGKSTTARAIDQLYKPTAGEVILKGKDLTKLPPSEMMEARKNIQMVF
                     QDPYASLNPRMTAGDIIAEPIRIFQKQGLIDMSAQDRNNRVEELMEKVGLSRYFKNRY
                     PHEFSGGQRQRIGIARALALNPELILCDEPVSALDVSIQSQILNLFKDLQGEFGLTYL
                     FIAHDLSVIKYISNRVAVMYLGLIVEIADAADLYKKPLHPYTEALLSAAPIPDPKIEA
                     KRKRIILTGDVPSPDKQRPGCNFYDRCPKRMDICKERRPSLKDSEVGHAVSCFIYHKP
                     E"
     gene            17643..17879
                     /locus_tag="PLQ30_02705"
     CDS             17643..17879
                     /locus_tag="PLQ30_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01384.1"
                     /translation="MFHAKRLNEAALRDPRLLSSYPVNSSLYQKSNLCLYPGKFPSQS
                     YRSCQPQLNVSNLSVSLRDAVWRLIMIFYPGGFL"
     gene            17876..18673
                     /locus_tag="PLQ30_02710"
     CDS             17876..18673
                     /locus_tag="PLQ30_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HPW01385.1"
                     /translation="MKRKIVTFIFLASLVSTMLVFAGPFSSGTLRVGATSVPHAEILN
                     FIIPDLAAQGIKLEVIEFSDYVTPNIALAEKQLDANFFQHLPYLETFAAERKLSLESV
                     IAVHVEPLGLYSKKYKKVSDFPSGSIIAIPNDPTNEGRALLFLQSKGIIKLKPDAGLQ
                     ATIRDIVENPKKFVFKEIEAPQLPRTLDDVAGSIINGNYALQSGFNPIKDSLLLEGGE
                     SPYANILVVRKGDSNDPRIQALAKALTSQRVKDFILNTYKGGVIPAF"
     gene            complement(18759..19703)
                     /locus_tag="PLQ30_02715"
     CDS             complement(18759..19703)
                     /locus_tag="PLQ30_02715"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF013991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HPW01386.1"
                     /translation="MYWDDLIAQVGNLALTATLYEVSAAPKPGLVDPFSRGAHSDMDY
                     FTFLASAAALAPFFVDFARIGAEFAGESPGLLAELRKLGIEAEKAMYAATQGINTHKG
                     LIFSMGLACAAAGKLLVSDRRAEISDRSAVISGCIQEVADIVSGITVRELVPLVEAEM
                     LLASDTIRANNAVVPSIDLSAGEQSFIKYGIRGVRGEAEDGFPSVINHALPRLHFDLL
                     AGMSWNDAMVDALLILYLKVDDTTVLKRAGSDGLAYVRAQAAAALSRGGMRLMENRDF
                     VQKLDADFSQRGISPGGCADLLAVTAFLEMLSSKWIAQ"
     gene            complement(19748..20296)
                     /locus_tag="PLQ30_02720"
     CDS             complement(19748..20296)
                     /locus_tag="PLQ30_02720"
                     /inference="COORDINATES: protein motif:HMM:NF015742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HPW01387.1"
                     /translation="MLSLQTVLAARELRWKEEELLAKSLAQEFSGHRLAFAFCTLRMP
                     ASLRISGPYKEIVVQLRDALAEALMEKAFPIIASGQKQYDDGPSAWLASCCDEEILKR
                     MAISIEEGHVLGALGDIDVTDAEARLLSRRDMGFEPRKCLVCDNEAMVCSAGQCHSLL
                     DIAAHIEAIISDSMALRSSKSL"
     gene            complement(20290..20559)
                     /gene="citD"
                     /locus_tag="PLQ30_02725"
     CDS             complement(20290..20559)
                     /gene="citD"
                     /locus_tag="PLQ30_02725"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HPW01388.1"
                     /translation="MRVLKSVTAGSLESSDVLVTITPVSEDHIEYDIESIVIKQYGKR
                     IRQITEEMAESAGITGARIHVQDRGALECTLRARLETAIERSIVC"
     gene            complement(20603..21760)
                     /gene="citC"
                     /locus_tag="PLQ30_02730"
     CDS             complement(20603..21760)
                     /gene="citC"
                     /locus_tag="PLQ30_02730"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR00124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HPW01389.1"
                     /translation="MPRVEGYRTGRLENIIGKHMSELNFGLEERILNSKDELAQREAL
                     LTSRGLMVPEGEELVLGLFVGEQLVATGALVRNVLQGIAVDIDYEGEGISAAIVSSLI
                     RRAVDRGITQVFLYTISEDISRFESLGFRKVAAVPRGAALLEWGTAGIESYLASIRAL
                     AKDKPDHAGAVVINCNPFTLGHRALIEYASSHVPWLYVLVVEEDRSLFPFKVRYKLVQ
                     EGVADLRNVTVMKGGPYIISSATFPTYFIRPDVNKASWKAVRMHASLDVSIFRTHIAP
                     ALKVTDRFVGTEPYCPTTSIYNDIMKIILQAKETEAFGLDAKTPPVALHEIPRFELWG
                     TPVSASKVRDMIREGNLSEVQKFVPGTTWDWLKSEEAAPVIERIRQSDSRH"
     gene            complement(21729..23669)
                     /locus_tag="PLQ30_02735"
     CDS             complement(21729..23669)
                     /locus_tag="PLQ30_02735"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HPW01390.1"
                     /translation="MGKNIVQKLIDSHRIEGVQKSEFEDSEVLIKIDQTLTQDTTGTM
                     TYLQFEALGIDKVRTELSVSYVDHNTLQVGFENADDHNFLQDIASRYGIVFSRPGNGI
                     CHQVHLERFGVPGKSLLGSDSHTPTAGALGMLAIGAGGLDVALAMAGLPFSIPRPRVL
                     KVELRGRLSPMVSAKDIVLEVLRRLTVKGGVGKILEYAGEGVSTLSIPQRGTIANMGA
                     ELGATTSVFPSDEVTKRFLEAQGRAEDFIPLFADSDASYDEEMIIDLNTLEPMIALPH
                     MPDNVVPVHEIAGLPVDQCFIGSCTNSSYYDLAVSASILRGHHVAPQTSLVVAPGSRQ
                     VLLMTARDGILTSFVEAGARILECACGPCIGIGQAPATNAVSLRTSNRNFEGRSGTKS
                     ARLYLASAETAAASAIAGVITDPRTVKSRVTISIPESFINDDSMFIFPSTEGEKAGIR
                     RGPNIAPLPQFEAIGDELRAEVLMHMGNNITTDHILPAGSRIMSLRSNLPAISEYCFS
                     VLDADFPARARAAGSGILVAGQNYGQGSSREHAALSPRFLGIRAVIAVSYARIHRQNL
                     VNAGILPLIFDNSEEIQNISQGDMLYIKNIHEGLRLDRFEVKNESTGTTFMAHHGLSQ
                     RQIEIILAGGALNAARRRLSNR"
     gene            23807..24517
                     /locus_tag="PLQ30_02740"
     CDS             23807..24517
                     /locus_tag="PLQ30_02740"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPW01391.1"
                     /translation="MSLLQEVPDLHIQPMREVIYERLRKAIVLGELQPDSYFTDADIA
                     HEFGVSRTPAREALQKLESNGYIERVPKKGNRILGISPYELAHSYAIRKALETLAVRY
                     SAIRITEAELVEMKEVLDKIYEIRQSMTGDELLENLLPSIKRFNEIAFEACKSSRLTE
                     AVWSEREIFDRYRVLRMVFSNRIDKSIHRRNELYKAFLAHDPDLACRIWTDHLNESFL
                     IWREKSGNSEVLKDFQFF"
     gene            24587..25567
                     /locus_tag="PLQ30_02745"
     CDS             24587..25567
                     /locus_tag="PLQ30_02745"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HPW01392.1"
                     /translation="MKKLSLLVLTVVLLGLVATVGAAPAWKPTKTIELVAPANPGGGW
                     DMVCRVIQKALTEEKLVDKPVIITNKPGGGGSVGWTYLKGKKGQGEYLAATSSLLILN
                     NLLGTSDLTYKDFTPIACMQAEWEAIVVREDSPWKTVNDLMEAIKANPGAVPIAVGPS
                     LGNDDHLVFLQLARTYGVNPAKVKFIVYPGAGGEEVPALLGGHVKAMVIGLAEVLEQH
                     RAKKLRVIGVSSPERLELLPDVATIKEQGIDIVFPHWRGIIGPPGLTPEQVAYWDEVF
                     TKMVQTKTWKEQLENLLWDDYYQNSSDHAKFLEEQTKVFDELLTAVGLKK"
     gene            25656..26132
                     /locus_tag="PLQ30_02750"
     CDS             25656..26132
                     /locus_tag="PLQ30_02750"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HPW01393.1"
                     /translation="MTTNILTGIISIVLGLVYSITALRLPRMAMGDRLGPKIFPYFVG
                     IVTIITGIALILQDRDPEKRSKKAEFGFKEHKNLWLNIVILTIAGILFGFFIDELGYM
                     IATALFIFSVSMLINKGKLVQNIVIAVIFPVVTYFVFGVALKLSLPRGIIETMLPF"
     gene            26144..>27008
                     /locus_tag="PLQ30_02755"
     CDS             26144..>27008
                     /locus_tag="PLQ30_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HPW01394.1"
                     /translation="MQSILTNLSIGLQTAFTFNNILWVFIGGTLGTIIGMLPGLGPAT
                     GVAILIPITFGMNPATALITMAAVYYGAMFGGSRAAIMLNIPGDASAIVSCFDGYPMT
                     KKGKAGTALAISAIASFIGGTIGMIFLIFLSQPVASAALKFGPAEMFSLMAFALVATI
                     TLSEGNLLKGLFSIVVGLMLSTIGIDPLAGVVRFTYGVSSLNEGIDFLVIIIGVYAVT
                     EVYKNLKSIDRVYEFDSKSIGKVKVSKEEFKQCIVPMLRSTPLGFIIGVLPGMGGTIA
                     TFMSYAMEKNLS"
BASE COUNT         7253 a         6471 c         6280 g         7004 t
ORIGIN      
        1 gttgctcagg tcatctcgaa tgtcatcgat tttggattcc cgatccagga agcgattctt
       61 gcgccaaggc ttttccaggc tgcttccgga agcttgaatc tcgaagggcg gtatccagtc
      121 agcgcactcg atggaatcaa gaagctaggg catgatgtta ctattcgcgg cgactgggat
      181 tcctactttg gcggagtgca cgcagtgctg tatgattata acgaaggatt gctctatgga
      241 ggtgcggacc ctcgtagaga tgggcaggca gcggccttct gagctaataa tcacggcaaa
      301 actgcatcgc attagactgt attagacaat agagccggcc gctgagatag caggccggct
      361 ttatttagag ctttgttcca gtctatattt ctctggagct tcattggttc tgtatcgcat
      421 aaagagatct tatttttgtt ttatttggaa agactatatt ctaaggaaga aagggaatta
      481 aaatgaaaaa tgcaagaagg attattccaa ttctcttagt gcctctgttg ttttttggag
      541 gtggagctat actgccgctc gccgcatata atctcaagac ggcttttgcg ccgcagccag
      601 ctttgctcac ttctataggt cagagcgctg atgtggaaat ggcaaagact atcatggcaa
      661 gggcaaaatt aaatttcacc atggatccga caatcaaggc tcaagccctt gcgagcacga
      721 atgccaagac gttggtaata gttattggag gctcatccaa gggactgggc gcagcgggta
      781 tctcggcaga agcagaactc gaacggacaa aagccctgct cgccgaggct aaaaaacgcg
      841 gtatgaagat aatcggcctg catatcggtg gcgaagctcg gcgtggcgag ctttcagatc
      901 gcttcataaa tgaggcagtc ccttcctgtg actatttcat tgttgtcgag gaaggcaata
      961 aagatggcct ttttaccaag ctgtgcggaa ataagattcc tctcgatatt gtgcaaaaaa
     1021 tctcacagat cggcgagcct ttggctgcgg ctttcctaaa atagcgaggt ctctgtgaca
     1081 caggaattat ggcttttcat tctgatggtc ggcgcatttg cttctggatg ttttttattc
     1141 aaatggccgg tgtcagttgc aatgctagtc gcctcagcac ttgcagcaat tgtcggtggc
     1201 gtacaagatc cgattcggca cttggtagaa ggtacatttg gctatctcga tacaatcctc
     1261 acgattgcga cagccatgat gttcatgact ggcttccgcg attcgggagc cctgcaagcg
     1321 ttgacggcgg cagtgatcaa gaagttcaat aagcagcctg cgcttctgat gattcttctg
     1381 accctggtga tcatggtacc agggatgatc acgggtagtt ctacggcatc ggtgctgaca
     1441 gccggtgctg tagttgctcc gatgtttatt gcaatgggta tgtctaccat agatgcggga
     1501 gcgcttattt cgattgcggc aattttgggg atgattgcgc cgcccgtgaa cattcccgta
     1561 atgatcatcg gtgggggcgt cgacatgcct tatgtcggct tcgaaatacc tcttattctc
     1621 ctaacgttcc cgcttgcaat agtatttgcc ctctggttcg gttggagaaa tgtgcgtaag
     1681 gttgaccgtt cgcatgtgct tgcatcgctg gattttacta cactgaacaa atatggcgct
     1741 cttaggctgt tttctccgct catttttatc tttgtattga tgattctaga taagactgtg
     1801 ccacatatat tcggttttgg catgccgctt attttcatgt tgggaacagt tctcacctgg
     1861 gtaacgggaa cccgcttcaa catcttgaaa tctgcaaaga aatcgatcgg agatgtgctc
     1921 cctgtactgg gaattctggt aggcgtaggc atgcttattc aagtaatgac ggccacgggc
     1981 gtgcgcgggt ttatagttgt aaatgcattg tctgtgccct cttcgcttct atacttgatg
     2041 atcgctatct ctattccact ttttggcgcg atcagtgcgt ttggctccgc cagtgtgctc
     2101 ggagtgcctt ttatgctcgc attgcttggt catgaccaaa ttactacagc ggctgcgctt
     2161 tcgctgatag ctgctattgg cgactttatg cctccaacgg cgcttgcggc catttttgcg
     2221 gctaaagttg ttggcgttga gaagtatggg agcattttga aaaagctggt tgtccccgcg
     2281 ctgataataa tagcctgggc attgtttttt atcttgttct ccaagcagat cagcgctctc
     2341 tattagaaag agtcgaagga aggtgtccat gttttatatc tatcttggga ttctggcaat
     2401 tgtgctggct ctgacggtat gggagctctt cgatacgaag aagctttcta tgcagattac
     2461 ggcagcgatg gtgattatgc cgcttctatt aaggcttctg atgatcaagt aagcgaggga
     2521 tttccatgca gaaacataca ataacagcga ttatatgcct tgcgggagct attgtggctt
     2581 ctgcaatcgc aggctcttct tttctggcaa tgcgaaagcc ggataaaata gtaaggggac
     2641 ccggtgttac cgaaataaaa atgctttctg agtggtttcc ggatctgaaa gggagccctg
     2701 gcgatacaga agtctatatt atggaaggag ccgataatgg agtctccatg ctggtgcttg
     2761 gaggtactca ccccaacgaa ccagcgagcc atcttgccgc cattttgctt atcgagaact
     2821 tcctacctag aacaggtacg ctctatgtga tccctcgagc aaatgcctcc ggttttacgg
     2881 caaatgatcc acaggaaggc gctccacagc attttaccat cgaaacacca aatggtttac
     2941 gagtctttag ctatgggtcg agagcaacca accccataca tcaatggcca gacccaaaca
     3001 tctatattca tgcttcgtca ggtcagacac tctcggggag tgagacccgc aacctaaacc
     3061 gcggttatcc gggcagagcc gatggcacac tcaccgaaaa agtctgctat ggaatagttc
     3121 aacttatcaa ggagcagaag gtcactataa cagtggatct gcacgaagca tctcctgaat
     3181 acccggttat caacgcaatc gtagcacatc caaaagccat gcaactggca tcggatactg
     3241 tgcttggcct cgaaatgcag gatataaaga tcagtctcga gccctcgcca gtcaatcttc
     3301 gcggacttac acaccgcgaa ctaggagact tcacggacac tctgcctctc ttgatggaga
     3361 ccgcaaacgc ttcacaagga cgattgcgtg ggcgtacaga cgaagctctt gtgttgaccg
     3421 gcaaggataa atggtatgtg cgcgctgcaa agctcgggcg cctctatgtt ccatacgatg
     3481 agaatggcca tcctattgaa gaacgggtgg gaagacatgt tacggcaata gccgagatta
     3541 tcaattcctg gaattgggag catccggaaa cacccttgga attcgacaat attcccagct
     3601 atgaggattt gcttagtaag gggcttggag aatatctgct gcctgtgcaa taagacccaa
     3661 aaatggagct aaactaagcg ccctacttct gagttatgag tttctcgatg tgtctgcggt
     3721 gtttttaggg cggtgcgcat tttagctgtg gacatagaaa tttgtcgcat tatgtagctc
     3781 gcggcaccaa aaaacagaaa actctgagca ccgaacaaaa tgcgcaccaa attcccctat
     3841 ctcccaaact cccggcggca atgaagctca tcaacgagcg ccgaacaaga tgcacgctaa
     3901 atcttcctat ctcgctggat cctcgaattc ttggctaatt actcattaca agaataggtt
     3961 ccagcgcttc atcgatcttt tctaggattt cagcgttgag tctgggcaga atttcgatag
     4021 ctccgaggtt ttcctcgagc tgggaaagcc ggctagctcc tgttatcact gtacttacat
     4081 tggggttgcg tgcggcccag gctatggcaa gctggctcat ggttgaaccg atggattcgg
     4141 cgatagggcg aaggcgttca atgcttgata ttatttctgg ctgataccaa cgctcttgaa
     4201 gccactcata gccatacagc gcaaaacgcg aatccttggg aataccttcc ttgtacttgc
     4261 cggacagaag gcctgaagca agagggctaa aagtagtgag ccctaggcct atcccctggt
     4321 aaaggcgcgc aaactccttt tcgaccttgg tacgcaccaa catgttgtat tggggctgtt
     4381 ccatctgggg ttttcggagg ttgtgcttgt ccgcagtctc ccatgcttgg cgaatctgtt
     4441 cggcagacca ttcgcttgtt ccccaataga gagcttttcc cgaggatatg atatcgtgca
     4501 tagcccagac cgtttcctct attggcgttt ctgggtccgg acgatggcag aacaggaggt
     4561 cgacatattt catccccaga cgctcgaggc tgccttcgat tgcctcgaga aggtacttgc
     4621 gattgagagt gttcttttca tttgggcctt catgaagccc ccaataaagc ttggttgaaa
     4681 caagatagga gcctcgtctc aagccaagcc tgcgaaaggc ttgacccatg atgacttcgg
     4741 attgcccgcc tgcataggat tctgcattat cgaaatagtt tacgccatgg tcgtaagcat
     4801 atttgatcat ctgagatgcg ctgtcgatgt caatctgcga gccataggtc acccatgaac
     4861 ccaaagaaag cactgatacc ttgatacctg cggaacctaa atgtcgataa atcatagaat
     4921 agctcctttg cggaagaatc ttctgtggca atgtccggag caagaatccg cgtcaatact
     4981 gagccatgct ttgagccatg ctttatgcaa aaatagtaac ccaataaatc ataagctcac
     5041 tgccgaaaaa tgcataacaa tcggactttg ctctatttcc cttgctccga attgagccca
     5101 cattaaaaac atactaaact gagttcagaa agggaagatt ctgaatgtca aattctgata
     5161 gaatattcaa gattgtccat atttccgatc tccatcttgg aaagactctt agatctcaag
     5221 atctagctca agatcagagc agtatactca aacaaattgt tgaaaaggtc aaaatgctca
     5281 agccagatct tgtcatcatt gctggcgatg tgttcgatcg gagcattcct tcagagagtg
     5341 ctcagactat gttcggctgg tttatggcag agcttagacg cgcgttaccg aaagacggac
     5401 gcattctagt tattcctgga aatcatgatt cacccaaacg aatcgctttt gccgcagaat
     5461 tgttcgaatc tgtaggcata cacatagtca gcgaaatcct atcaaacccg acactcgtgc
     5521 taaaaaaaga tggaaaaaga gccgcagtct gggcgctgcc ctttgtcacc catggagcct
     5581 accacgagtt taagaggaac ttgaccaaaa cgggctatgc agaaatggct tctgcggagg
     5641 cgaatagctc aatggcaggt cgcatggaga gcattatagc aaacctccgt ccctccaaat
     5701 cagcctccga atccgaatac gacctgaata tcctcgtggc gcactgctat gttaaggagg
     5761 ctgttctatc cgaatcggat atcgccttta ttggcggaac agaagccgtg ccggcctctc
     5821 ttttcgagag cttcgattat gtagcacttg ggcatctgca taaaatgcaa gctctgtctc
     5881 cgaaagtctg gtattctgga gcacctatgt ctatgtcctt tggagatgga gatatcgaga
     5941 aaggatttct atatgtcgag ctcgaggatg gtaagccggc aagagtacag cgaatggaac
     6001 tcaagccctt gcatgtcttc aagcgcgtga gaggcagttt tgacgaactt gctgccgaat
     6061 ctgctcttat agatgatgat tatgtcgaag tgatccttac cgacgaagaa ccagtatacg
     6121 atgcttttaa tcgtcttgct attcgttttc ctaatttgat gacggtacgc caggaagcct
     6181 ttgaaaaaag aatagggaca gaggttgaag aagtcggaat tccggttcct catatagttt
     6241 cggatggaga gacagaaaat actgatgctt tggaagattt ttccggcaaa tcgcttcatg
     6301 ctacggttct caaggatttt gttggcttta cgcagcgcat tctcgatgag ccgccttctg
     6361 aggaattgct gaatgctttc gaggtactcc ttagaaatgc ggaagaggac ctcctatgaa
     6421 gccactttat ctttctttgg agaactttgg tccatatcgc cagcgtcagg acatcgattt
     6481 ttgtaagtta gatgattttt tccttatcta tggcaagact ggttctggaa aaaccacaat
     6541 cttcgatgca attacctatg cactctatgg caaggccatt gggggacgga gcaatctcga
     6601 gcgggaactc ggatcccgct tttctccaat cggaagcaag ccctgggttg aattcgaatt
     6661 cttggcttca tctgcccaat ggaaggtgta tcgaagcttg ccctacaaaa aacgtaataa
     6721 aagaggggtt gaatccgagg ctaccagcga agttgagctt tatcggatga atgaaatcgg
     6781 ggtctatgaa ataatagccg atcgctctac tacggctaat caaattctgc tcgatctttt
     6841 gcgtctgagt gccgatgaat tttcgaaaat cgtactcctg ccccagggag agtttcaaaa
     6901 atttctcgaa atgaagacca cagagcgagt ggagattctc gaaaagctct tcgatgtggg
     6961 tatctatgat gcggtaaccg aagcagcgcg aaaaaaagtc atggagctag aggcttatct
     7021 taaagcaaag caggaggaaa tcgagcgcat ctcgaatgaa ctcggcatcg agcctggatc
     7081 tcgcataaaa gaatcgggaa aagccatctc agaattcgat aaaagaatcg aagaatccac
     7141 gaaagacgct tcgcagttgg aaactcaaat cgctcaaaat agagagcgtc tttcattcct
     7201 gcaatcaatg cttgcggcct ggaaaaatct tcatgaaaaa gaagagctaa agcctgtctt
     7261 cgataaacgc gaaacaagac ttgaagctgc caaatcgctt ggagaactgg caagcctggc
     7321 taagcaagtt tcggctgatt acgaggaatt aatcgagctt ctttcagaat ccttacaaac
     7381 agggacagag ctttccaagc tcgaagcaga agggatcgag atcaaaaaga tacagaaggg
     7441 actgcccatt gttcgaaaga aatacgacaa tctccagcgc agcgtggcat tatatcagaa
     7501 tgcagtgaat gcatggaatc aaaaagagca atgcgaaagc gagcgcagac aagttatgca
     7561 gagactcgat gccactttgc aggattggga atatcgacaa aacgagatcg gtcagctcaa
     7621 atccagcata acagagatcg aaaacgccat acaaagcgag cccttggcac aagagtcatt
     7681 tcataagatt gtcatcgcgg atgaagcatt caaacgaata gagacctgga tagaacaaaa
     7741 agaaaaaata gaagctcaac gcgtggaaac cttacgtaaa attcgagaag cggaagattc
     7801 tatccttctg acaaaccagg agctcacaat tgccgaacgg aatcgagatc gcatcaatga
     7861 ggaaatccga aaggctcaag ccggtatgct tgcggcctcg cttattcccg gagaaccctg
     7921 ccccgtatgc ggtgctcggg atcatcccaa tcttgccaaa cttgaacaga atgctccgga
     7981 tgacaagact atcaagaccg cccaaaaagc ggtggaagag accaaatcat cgctctctgc
     8041 cataatccaa gacaagcaaa atctcgaaaa ccgcatcgaa gaattttcgc aggatcttca
     8101 agttatagtc caaaaaatct ctgaggaatg gaaaggcatt gcttgcgaag tgattggtga
     8161 taaaagcaca atgcagcttc tggatatctc tccctcagag ttcgtatcca taagctcacg
     8221 cttgcaatct gagcgtcgcg agctagaaga acgactcaag agttatcaac atggacgcaa
     8281 atcattacaa gaacttagaa agactcttga tcaggcaggg cgagatcttg atcgaattca
     8341 agaggaaaag acaagactcg agatgaagca gactgaatta gatgcacata ttgattctct
     8401 tggagagcag atcggaaaag aggatcctcg gatttgccta aaagaagcag aagaaaatct
     8461 tgtcactctc cataacgaaa ttgagcagat cgaagagcaa gcacaaaaat gggaaattgc
     8521 ctggagctcc ataagtatca agatggacat gcagttggag aaaattggcc aaaaaggccc
     8581 cgctcttgcc acaatggtcg aggaattcct caatgaggtg gacaaaatga aagtgaggag
     8641 cctattgcgg cttctcgaat ctccggtgac ttcttcggaa aaagaagcac cagaacatga
     8701 gcaacaggca atccggccat tgccagatct cttaacttca tcgggtaata atgcgggtaa
     8761 tttatcgggt ttttcagagt ctatctatga gccttcccca agactgctgc aggagctctt
     8821 tgccttgcta gctaattcct taccacatat cgatagattc gtaccggtct ccgggcaaat
     8881 tatgtacctt tctgagaccc tggagaaaat cttgtctgct catcgaataa atgagccggc
     8941 attaaaagca ctgatgcaag aaattctctg ttcggtgtgg acaagcaatc gcatcaaaga
     9001 agaagccagg gagatttccg ctttcagaca atcctataat gaagctagta tttcctatca
     9061 agtgctcgcc gcgcgcgcac aagctctgtc cccattcaac tcattgtccc ccaactcatt
     9121 caattcaact acgctccccc catcaaacca atcgctagat cgattgcccc aacataatca
     9181 agaaaatggc gaccaagcaa aacatcagga aatgttcgat ggcattcatc tcgagcaaca
     9241 agaaaaaaac cttagagagg aatgtctttc ccttctcgaa aggaagaaga gtattgaaac
     9301 ggagatatcg gatttgcgag acgagcgtgc gcgttttata gcggagctcg atcagcttcg
     9361 tcgccttctt tcaaggcgag atgaattgac tagcgaatat gaaagggcta tcaaggagtt
     9421 cggaaaacac caagagcttt caaaactgct tggagggagc ttaaaccctc aaagaaaaat
     9481 gccattcaaa aactatgtgt tgggccttca attccgagaa atagccatga gggcttccag
     9541 ccgactctat cggatgagca atggccgttt cctggtggaa gccgatattt tgggcggcag
     9601 cggcaaccaa aaaatcggcc tggagctcta tgtgctggat gcctggaatg ggggtcgacg
     9661 cccagtgggg acgctttccg gaggagaaaa attcatgctc tccataagcc ttgccctggg
     9721 gttagccgat tcgatacagg aacgagcaag agcaaatcgt atcgaatcgc tttttatcga
     9781 tgagggcttt ggaagtctcg acgaggagtc tctctcgctg gctatttctg tcttagagga
     9841 ccttagaggc gacaagagca ttgcgatcat atcccatgtg gatgagctct atagccgtat
     9901 accatcgaga atcgtggtca aaaaatctgt agggggctcc catctagagt tagaacaaga
     9961 ttaaaggctc ttctgttcat tgctgaggcg ccccatggaa ggcgcgctcg agcagttctc
    10021 tgagttcttc atcagtaaaa gcacgtgggc tggccttgcg cctacgataa gaaagagcat
    10081 cgctaagtat atcttcgaaa taggacggag atacgccggc agcttcaagg ttctcaggga
    10141 tacctatatc atggcccagt tggcagattg cgcgcaccga ttcttcgggg ctgatattgt
    10201 ccttagccgt tagcgcttgc ccaatgtccg ataggcgtcc ttttgcgtct ggaatcaagg
    10261 attgcatcac aaaaggaagc atgatcgcat tggcaagccc atgtggtatc ccagctaatg
    10321 cccccacggc atgggcaaaa ccatgaacca ttcccaatcc tgcctcggag agagctactc
    10381 ccgcaagggt cgccgacagc gccatgtcgc gtctcgattg catggctttt gctccctcgg
    10441 atatatggca tgccgacctc aggctacgac ctataagacg aatggattca aggcagtaca
    10501 tgtcgctgat gggcgtgtta aaacggctca cataggcttc tatagcatgg gtaagagcat
    10561 ccatacctgt agctgcggtg agagaaggcg gcatgcttaa atgcaaaaca ggatccacaa
    10621 ttgcaagctt ggggaaataa aactgactcg aggtgccctt tttgcttcga cgaccgctct
    10681 cgtccactac ggtgaatacc gcggcatttg taacctctgt ccccgtaccg gcggttgtcg
    10741 gaattgcaat gagcggtgga acgggctttg taaatgccaa ccccgttccc acaaaatcgc
    10801 ggatagaacg accttcggca acgctggctg cgattccctt tgcacaatct atagggctgc
    10861 ctccgccaaa accgataatg gcgtcacaat gctgcgctct ataaacctcc gtccctttat
    10921 ctacaagaga ggtatcagga tccgcgctga cttctccaaa atgaacatac cgtatgccac
    10981 ttgcatctaa gccgatgcac agctctctga aaccctgcaa agaagcggtt ttcgatctgc
    11041 cgctcacaat cagcacattg gtcgctctca tttctttgag tcgcggggaa gcttcggcaa
    11101 ggcagccatc cccaaacaat accttggtcg gtgcatagaa ttcgtacata gcatatctcc
    11161 tatagaatta gctttccgaa attgcctatc ctatcaagta taaacaagtt tccaaaggag
    11221 tcttcagcaa agagcgctgc aataaaggaa gctcaaatca gaactatatt tctcaatcat
    11281 gtcataaatc cccaaaaatc gttgagcttt atacctaaaa tcttaaaaga ttcttgcatt
    11341 gctcataaat aaagtatata gtatttgaga atgtaactca aagaggaggt aatatgaaaa
    11401 agatcctttt cgcattgatc gcactggttg cggtcttagg tcttagtgcc caggacttta
    11461 caatcgtaaa tggtactgag cctacctcgc tagatccaca tgcggttgaa ggtgtgcctg
    11521 agcaccgcat ctatgtggcg cttttcgagg gtctgaccat caatgatccg cgtaccaacc
    11581 gagcgcttcc tgcagttgca gagagctggt cttttagcaa ggattacaag acctgcacct
    11641 tcaagattcg caaaggtgtt acatggtcgg atggcgtcga aataaccgcc gatacggttg
    11701 ttcagtcctg ggtgcgcaag atggatccac agaatgcctt ccagtatgcc gatctacccg
    11761 caatgtatat cgaaggtggt atggattatc tcgaaggcaa ggccggtcca gagagcctta
    11821 agatccgtgc tgtggataaa tacacattcg aggtcaaact ggttggccct attccattct
    11881 ttgcagatat ggtaagccac tatgccttcg ccatcgttcc tatgcacgct atcgaaaaat
    11941 atggcaagga ttgggtaaaa ccaggcaaga tggtatcgaa tgggccttat gtcctatccg
    12001 aatggaagcc ccaggaaaaa gttgttgttg taaagaacaa caagtactgg gatgcaaaga
    12061 acgtctctct caagaaggtt accttcattg ctaacgacga catcaatgtt ggctataacc
    12121 tctacaagag tggagccgct gactggacag aagccgtgcc tctagaactc atggatgagg
    12181 tcaagctacg caaagacttc cacgttgctc ctcaatatgg aacctattat tttatctaca
    12241 acacaacccg aaagccgctc gacgatgcac gcgtgcgcaa ggcgctcgct atggctatca
    12301 acaaagacga ccttgtcaac aaagtcaccc gtggtggcca gatacctgca aatagcttag
    12361 tgcctcctag caccggctat actccagcaa agggttacaa cttcgatcct gcggcagcac
    12421 gcaagcttct cgcggaagcg ggctatccgg atggcaaaaa tttcccggca tttacgatac
    12481 tctataacac ttcttcgtct cacaagagca ttgcggaatt catccaagcc cagtggaaag
    12541 ttaaccttgg catagatgtc agccttatga accaagagtg gggtacctat ctcgacaccc
    12601 gatcccagtc tcataacttc gatatcgctc gagctggatg gattggcgat tatctcgatc
    12661 catcgacttt cttggaaatg ttcataactg gcggaaccca gaatgatggt ctctatacca
    12721 acccgaagta tgatgaactg ctcgacaaag cgcgtgttgc gagcggcact gagcgcttcc
    12781 agtatcttat ggatgcagaa aagatcctca tcgatgaaga tatggcagtt cttccgctat
    12841 atttctatgt gacccagaac ctcatcgatc tggacaaatg ggatggctgg tatgcgaacc
    12901 cgctcaatat ccatccatgg aaatttatcc gacccaaaaa ataaaaatta cctaaagtaa
    12961 gtaagcaggg ctgtccgata cgggcagccc tttttttctt gggcaaattc ttgcagtatc
    13021 aattctaagg aaatattggc tagataaaat ttcatgattg gagtataatg ttttcgcttg
    13081 agaaaagcct tctcatgttt cgagattcca attgcgcaaa tatattcttt tagaataatc
    13141 aatttgcgac tcggaaagga gtaattccga atgccacgat atttcattcg gagaatattg
    13201 agcataattc ctacgctctt tatcgtgatc acactgagtt tttttctaat caggcttgca
    13261 cctgggggcc cttttgcccg cgaacgtgag gtgccagagg cgattcttca aaatctcatg
    13321 aagcgctatc acatggatga accgctcatt aagcaatatt tgcgctatat gggcgacgta
    13381 attcgttggg atttcggccc ttcctaccga tatagggatc taagcgtaaa tgagattatc
    13441 gacgaaggtc ttcccatatc gatgtccttg ggtgttatct cgttggtact ggcgacggtc
    13501 ggaggaattg ccatcggaat tatttcagcg ttgaagcaga acagatggca ggattatgtt
    13561 gtcacttcta tcgctgtcat aggcatttcg gtaccgttat ttgtgatggg gcctgtttta
    13621 cagctcgttt tcggaatgaa gcttaagatt ttgcctatag gtcaatggat ctcgactcat
    13681 ggtataaaag cggtaatcct tccggccata actctatcgt ttccatattt tgcctatatt
    13741 gcgaggctat cacgcgcaag tattctcgaa gtgctgcgat ctgattatat ccgcacagca
    13801 cgagcaaagg ggcttaaaga gtcagtggta gtttggaagc acgtgctcaa gggagctatg
    13861 ctccccgtag tcacatatct cggcccagcc ttctcaggta ttgtagtagg ctcaatagtc
    13921 gtagaatcgg tcttccttgt acccggaatt gggcgcccct ttgttcaatc tgccctaaac
    13981 agggattata cacttataat ggctgaagtg gttgtatatt ccattattct catcattgca
    14041 aatcttgtgg tcgacattct ctatggcttc ctcgatccaa ggatttctta caagtaaggg
    14101 gtgggagatg gcaaaaaaac aacttgaaac tgcagtcaac gagttcaacc aggtaatgaa
    14161 gcccgtctcc ttatgggctg acgcatggaa gcgccttcga aaaaatagga tggctttgat
    14221 aagtctcgta atagtatgta tatatttggt tatcagtatt tttgcgcctc ttcttacgag
    14281 tgctggaatt ctcatcgatt accgcaaaca agtcattacc aatgcgagcc tgcctccttc
    14341 tttcaaacca gccggagagc tggttatcaa taagatcgag aagcgcatcg cggatctcga
    14401 acaaagagtc gaggaacaaa aacagcagca gtctgccttc gattctttct tcgattttgg
    14461 atccgtgtat gctcaggatg aaaacggcga agccggcgat gatttcgcac caagccagga
    14521 ggaattctct tttacttatg aaggtgaagg attctttggc aataccaccg atccggtagt
    14581 ccgcgaactc atcagccaaa aagaaacctt agaacgcgtc aaagccgagc tagatacaaa
    14641 ccccgactac aagatcgtat acattcttgg aaccgataat ctaggccgag acatgtttgc
    14701 acgcataatc tatggtggtc gcatttcgat tgccatagga gtcgtaggca ctctgacagc
    14761 tgctttggtg ggtattctgg tcggagcaat ttccggctat gtcggtggct ggctcgataa
    14821 ttttcttatg cgttttgtag atatcatgta tgggctgcct tatatgctca tcgtaataat
    14881 catcatggct atgatcggcg agaaagctcg tgggagcttt gtggtgctct ttgtcgcaat
    14941 agcattagtc tcttggctta ctattgcacg ggtggtacga gggcagatta taagcctgaa
    15001 aaattcggaa tttgtcgaag cagcacggtc tatgggtgca agtactcaac gcattatctt
    15061 ccggcatctt ctgccaaaca ctctaggagt catcatcgta ttctccacgc tgatgatgcc
    15121 aagctttatc atgaatgagt cctttctgag ctttttggga cttggcgtgt cggcaccaga
    15181 tgcatcttgg ggcactctcg tgtcggaagg agttcgtgct atggaatcct atgcctggca
    15241 actgcttggt ccgggtatta caatgacgat ctttcttttc tgcatgaact tcctgggcga
    15301 tggccttcga gatgctctcg atccgcagag taagaaccgc acttgataag gagcctaaat
    15361 gtctgatgaa gtcatccttc aagtaagaga cttgaaaaca tactttaccg tagatgaggg
    15421 aatcgtgaag gcagtcgatg gcgtaagctt cgaacttcac cgcagtgaaa ccctcggaat
    15481 tgttggtgaa tccggatcgg ggaaaagtgt caccaatctc tcggtcatca atctgattcc
    15541 gacacctcca ggcaagattg tcgggggaga ggtgcttttt catggcactg atctgctcaa
    15601 attgccgccc ggcgaaatcc gccatatacg aggcaacaag atttcgatga tcttccagga
    15661 tcccatgacc tcgctcaacc ctttcttgcg aatttctaca cagatgatcg agacaataca
    15721 gctgcaccag gggctagata agaaggcggc aaaggaaaaa gccatcgaaa tgctcagact
    15781 tgctggcatc cctgctccgg aaaagcgcat cgatcaatat ccgcatcaat tttcgggggg
    15841 aatgcgacaa cgagtcatga tagccatggc cttatcatgt aatcccgaga tactgattgc
    15901 cgatgagccg acaagcgcac tcgatgttac aattcaggct cagatcctcg agctcatgaa
    15961 ggaattaacc cgaaagcttg gcaccgcagt tattctaatt acgcattcgc taggcgttgt
    16021 tgcgggtatg tgcgacacga tctgtgtcat gtatgcggga cgaatcgtcg aacgcggacg
    16081 caccgaagat atctttgaaa atccgaagca tccttatact atcgggctaa tccgctccgt
    16141 accacgcctc gatcaagaga ccaagatgcg tctatattca atacccggtc agccgcccaa
    16201 tgtaatagat ttgccagatt gctgcccatt ctacccacgc tgcgaacaat caatggatat
    16261 ctgcagaggg aaatacccat ctattgtatc cttcgagaac aatcagagcg tagcttgctg
    16321 gctctacaac aaggagactc agaatgcctg aaaccgacga agtacttcta gatgtgcgcg
    16381 gcctcaagat gcatttccct gtcaaaacaa gcatgttctc taagacgaag aattatatat
    16441 atgctgtaga cggaattgat tttaaggtaa gaaaaggtga gactctgggt cttgtaggag
    16501 aatcgggctg cggaaaatcg accacggctc gcgccataga tcagctgtac aagccaactg
    16561 ctggtgaggt cattctaaaa ggcaaagatc tcactaagct accacccagc gagatgatgg
    16621 aagcgcgaaa gaacatacag atggtttttc aagacccata cgcttcgctc aatccacgta
    16681 tgaccgcagg cgatataatc gcggagccca ttcgcatctt ccaaaagcaa gggcttatag
    16741 atatgagtgc gcaagatcgc aataatcggg tagaagagct catggaaaaa gtggggctgt
    16801 cgcgatattt caaaaaccgt tatccgcatg aattttcagg tggccaacgc cagcgtatcg
    16861 gaattgcgcg cgcattggcg ctcaatccgg agctcattct atgcgatgaa cctgtatccg
    16921 cgctcgatgt ctcgattcag tctcaaattc tcaatctttt caaagacctg caaggggagt
    16981 ttggcctaac ttatcttttt attgcgcacg atctttctgt cataaaatat atctccaaca
    17041 gagtggctgt aatgtacctt ggactcattg tggaaatcgc ggacgcagcg gatctatata
    17101 agaaacccct acatccttat actgaggcac ttttgtcagc ggcaccgatt cctgatccaa
    17161 agatcgaggc caagcgcaag cgcataatcc tcactggaga tgtgccctcg ccagacaaac
    17221 agcgaccagg ctgcaatttc tacgatcgat gtcctaagcg catggatata tgcaaagaaa
    17281 gacgaccatc actaaaagat tcggaagtcg ggcacgctgt gtcgtgtttc atatatcaca
    17341 agccggagtg atataggcaa gagagattcc tagatagagc ctgattagtt tcttgctggc
    17401 ctccctcaac agggctgagt caggtcaaga tgggggcaac acatacaaaa gacgaggccg
    17461 ccccgcccat tcgggcgggg cggccttttc ccataatcct tcaaaaggtc cctaaaaacc
    17521 actgcatcat aatatgataa tgatttccga tgactaattc ctatatcccg cttgacaaaa
    17581 aagaataaat aatgcatttt gcttcgaaag actaacgcca tgaaagctaa tagcaattat
    17641 atatgtttca tgcgaagcgg ctcaacgaag ccgctctccg cgacccgcgc cttctttcta
    17701 gctatcctgt aaactcaagc ctataccaaa aatccaatct ctgtctctat ccaggcaaat
    17761 ttccgagcca atcttataga tcttgtcagc cacaactcaa tgtctcgaat ctatctgttt
    17821 ctcttagaga tgccgtatgg cgtcttataa tgatttttta tcctggaggt ttcttatgaa
    17881 gcgcaaaatt gtcaccttta tctttctagc ctctttagtc tcaaccatgc ttgtctttgc
    17941 tggccccttt tcctcgggta cgctaagggt tggcgctact tccgtgcccc acgctgaaat
    18001 acttaatttc attatacccg acttggcagc gcagggaatt aagctagagg ttatcgagtt
    18061 cagcgactat gttacgccca acatagcact agccgaaaaa cagctggatg ctaacttttt
    18121 tcagcacctg ccctacctag aaaccttcgc tgccgagcga aaactctctc ttgaaagcgt
    18181 aatagccgtc catgtggaac ctcttggtct ctattcgaag aagtacaaga aagtttcaga
    18241 ttttccatcc ggttcgataa ttgccatacc aaacgatccg acaaatgagg gaagagctct
    18301 gctattcctt caatctaagg gaatcataaa gctcaagcca gacgctggac ttcaggcaac
    18361 gattcgggat attgtggaaa atccaaagaa atttgtattt aaggaaatag aagcgcccca
    18421 gttgccgcgt acactcgacg atgtggcagg ctcgatcatc aatggaaact acgctctcca
    18481 gtcgggcttt aacccaataa aagactctct tcttctagaa ggtggagaat caccgtatgc
    18541 caatatcctt gttgtcagaa aaggcgattc taatgacccc cggattcaag cactcgcgaa
    18601 agcgctcaca tctcaaagag ttaaggattt tatcctaaat acttacaaag gcggtgtaat
    18661 tccagccttt taatcggcct ctctgttcag gcttagtgat ccgacttatc gatcccgcct
    18721 tttgctcaaa aggagccttg cgctgatcca ttagcctttt actgtgcaat ccatttgctg
    18781 gacagcattt ccaaaaatgc cgtcactgct agaagatcgg cgcatcctcc agggctaatg
    18841 ccacgctgac tgaagtcagc atcgagcttc tggacaaaat ctcgattctc cattagcctc
    18901 attcctccgc gcgaaagagc agcagcagcc tgagctctga cataggcaag accatccgac
    18961 cccgctcttt ttaataccgt agtatcgtct acctttaggt agaggattag aagcgcatcg
    19021 accatcgcat cgttccatga catgcccgcc aagagatcga aatgtagcct tggcaaagca
    19081 tgatttatta cagaaggaaa accatcttcc gcttcgccac ggactcctct tattccatat
    19141 ttgatgaacg attgctctcc ggcgctgaga tcaatagaag gaaccacagc attattcgct
    19201 cttatcgtat cggaagcgag aagcatctct gcttccacaa gcggaacaag ttcgcgaacg
    19261 gtgattccgc taacgatatc ggcaacctct tgtatacatc ccgatatgac agcgcttcta
    19321 tcacttatct cggctcgcct atccgaaacc aaaagcttcc cggccgctgc gcatgctaag
    19381 cccatggaaa aaatgagccc tttatgggta ttgattccct gtgtagccgc atacatggct
    19441 ttttcagctt cgattccgag ctttcgcagt tctgccagaa gtcctgggct ttcgcctgca
    19501 aattctgcac caatgcgggc aaaatctaca aaaaatggtg ctagggctgc cgcgctcgca
    19561 aggaaggtaa aataatccat gtctgagtgt gcgcctctag agaaaggatc gaccagcccg
    19621 ggttttggtg ctgccgagac ttcataaagc gttgcggtca acgcgagatt ccccacttgc
    19681 gcaatcaaat catcccaata catcgatttg tctcaataat caatctcaat cgcgtgtttt
    19741 atggctatca taaggatttt gagcttctta aggccatcga atctgatatg atcgcctcga
    19801 tatgcgctgc aatatccaaa agagaatgac actgaccggc ggaacatacc atagcctcat
    19861 tatcacagac aaggcatttt ctgggctcaa aacccatatc ccttcgcgaa agaagtctag
    19921 cctccgcatc cgtcacatcg atatccccta gcgcacccag cacatgtcct tcctctatgg
    19981 atattgccat ccgcttcaag atctcttcat cgcaacaaga ggcaagccaa gcacttggcc
    20041 catcatcgta ctgtttctga cctgaagcta tgattgggaa agccttctcc attagggctt
    20101 ccgcgagagc atcccgtaat tgcaccacaa tctctttata aggaccagaa attcttagag
    20161 aagccggcat tcttagagtg cagaaagcaa aggctaatct atgccccgag aattcttgag
    20221 ccaatgattt tgcaagcagc tcttcttcct tccaacgaag ctcgcgagcc gcaagcacgg
    20281 tttgcaaact taacacacaa tacttctttc gattgcagtc tctaagcgag cacgaagagt
    20341 acattcaaga gctcctctat cctgaacatg gatacgcgct cctgtaattc cagcactctc
    20401 tgccatttcc tcggtgattt gtcggatcct tttcccatac tgtttaatta ctatgctttc
    20461 aatgtcgtac tctatgtggt cttctgacac aggcgttatt gttacgagca cgtcgctgga
    20521 ttccagcgag ccagcagtaa ctgatttgag cactctcatt gtcttctcca aatttttacc
    20581 tccgaaatta gatcaaaact tatcagtgcc tcgaatcgct ttggcgaatt ctctctatta
    20641 ccggcgctgc ctcctcactt tttagccaat cccatgtggt ccctggaaca aatttttgta
    20701 cctcggataa attaccttcg cgaatcatat ctcgaacttt agatgcactt acgggagtcc
    20761 cccacagctc gaaacgggga atttcgtgta acgctaccgg tggagtcttt gcatctaatc
    20821 caaaagcctc ggtctcttta gcttgaagaa tgatcttcat tatatcgtta taaatcgagg
    20881 tggtgggaca gtatggctcc gttccaacaa atcgatcggt gactttgagt gcaggcgcga
    20941 tgtgcgtcct aaaaatgctg acatcgagcg aagcgtgcat tcttaccgct ttccacgagg
    21001 ctttattcac atccggacga ataaaataag ttggaaatgt agcagaagag ataatatagg
    21061 gtccgccctt cattaccgtc acatttcgta aatctgcgac tccctcttgt acaagtttgt
    21121 atcgaacttt gaaaggaaat aaagatctat cctcctctac gaccagcaca tagagccagg
    21181 gtacatgcga ggatgcatac tctataagcg ctctatggcc aagcgtaaat ggattgcaat
    21241 ttattactac tgctccggca tgatcgggct tatctttggc caaggcccgt atggaggcta
    21301 aataggattc gataccggcc gttccccact ctagcaacgc ggctcctctt ggaaccgcag
    21361 ctaccttcct aaatcccagg ctctcgaacc tggatatatc ttcgcttatg gtgtatagaa
    21421 aaacttgagt tatccctcgg tcgacggctc ttcttattag ggaagagact atagccgccg
    21481 agattccttc gccttcataa tctatatcga ctgctatacc ctgcagaaca ttgcggacaa
    21541 gagcgcctgt agcaacgagc tgctcgccca cgaagagacc caagaccagc tcttcccctt
    21601 ccggaaccat aagtccgcgg gaggtcaaaa gcgcctctcg ttgtgcgagc tcgtcctttg
    21661 aattaagaat tctctcttca agcccaaaat tcaactcgct catatgttta ccgattatat
    21721 tttctaatct accggttcga tagccttcga cgcgcggcat ttagcgctcc gccggctagt
    21781 ataatttcaa tctgtcgctg tgaaagccca tgatgagcca taaaagtagt acctgtactt
    21841 tcgttcttta cctcgaagcg atcaagtcgc aacccttcgt gtatattttt gatatagagc
    21901 atatcgccct ggctgatatt ttggatttct tcggaattat cgaatattaa aggaagaatg
    21961 ccggcattta ccaggttttg cctgtggatc cgcgcgtatg agaccgcaat cactgcccta
    22021 atccccaaaa aacgaggcga aagcgccgca tgttcacggc ttgagccctg cccataattt
    22081 tgcccagcaa caagaatgcc gcttcctgca gcccttgctc ttgccggaaa gtctgcatct
    22141 agtaccgaaa aacagtactc agaaatagcg ggcagattcg atcgcagaga catgattctg
    22201 cttccagcgg gcaaaatatg atcggttgtt atattattgc ccatatgcat cagtacttcg
    22261 gctctgagct catctccgat cgcctcgaat tgcggcaagg gcgctatatt gggtccacgt
    22321 cgaattcctg ctttttctcc ttcggtcgat ggaaaaataa acatagaatc atcattgata
    22381 aagctctctg ggatcgatat agtcacacgc gatttgactg tgcgaggatc tgtaatgacg
    22441 ccggctattg cggaagcagc ggctgtctct gcgcttgcaa ggtatagcct ggctgatttt
    22501 gtcccggatc gcccttcgaa atttcggttg ctcgtgcgaa gagatacagc gttcgttgct
    22561 ggcgcttgtc ctattccaat gcaagggccg catgcgcatt caagaatcct tgctccggct
    22621 tcgacaaagc tcgtcaatat tccatccctg gctgtcatta gcaaaacctg gcgagagcca
    22681 ggagccacga caagactcgt ttggggcgca acatgatggc ctctcaatat cgatgcgctg
    22741 accgcaagat cataatagga tgaatttgtg cagctgccga taaaacactg atccacgggc
    22801 aagccagcaa tttcgtggac tggcaccaca ttgtccggca tatggggcag cgctatcatc
    22861 ggttcgagag tatttagatc gatgatcatc tcctcatcat aactggcatc tgaatcggca
    22921 aatagcggaa tgaaatcttc tgctcttccc tgtgcctcca aaaagcgttt agtaacctca
    22981 tcgctcggga ataccgaagt cgtcgcgccc agctccgcgc ccatattggc aatagtgcct
    23041 cgctgtggaa tgctcagtgt cgatactccc tctccggcat attccagtat cttgccaaca
    23101 ccgcccttta ccgtaagcct tctcaatact tcgagcacga tatccttagc gctcaccatt
    23161 ggagacaatc tgcctctcaa ttccactttg agaaccctgg gtctgggaat ggagaaaggc
    23221 aagccggcca ttgcaagcgc tacatctaac ccaccagctc ctatggcaag cattccaagc
    23281 gctcctgcgg taggagtatg gctatccgat cctaatagcg atttgcctgg aactccaaaa
    23341 cgttccaggt gcacctggtg gcatattccg ttccccggcc tagaaaaaac gattccatat
    23401 ctcgatgcta tatcctgcag gaaattatga tcatctgcat tttcgaaacc tacttgtagc
    23461 gtattatgat ccacatagct aaccgagagt tccgttcgca ccttatctat acccagtgcc
    23521 tcgaactgca gataagtcat tgtaccagta gtatcctgag taagagtttg atctatcttg
    23581 ataagcactt cgctatcttc gaactcagat ttctgcactc cttcgatgcg atgactgtcg
    23641 ataagctttt ggacgatatt ctttcccaca gaaaaatcct cccttcgatt tctgaaagat
    23701 tttgctttac aaatttcaga acctcgctta ttataccatt atacatcatg tatacggtat
    23761 acggaatacc gaatacaata aatttttagt gaggatataa gttattatga gccttctaca
    23821 agaagtccct gaccttcaca tccaaccgat gcgcgaagtc atctatgaac gccttagaaa
    23881 agcaattgtt ttgggggaat tgcagcctga tagctacttc accgatgcgg atattgcaca
    23941 cgagttcggt gtcagcagaa ctccagctag agaagcgctc cagaaactcg aaagcaacgg
    24001 ctatatcgaa cgtgtaccca agaaaggaaa tcgtatcctt ggtatttcgc cttacgagct
    24061 tgcacactcc tatgcaattc gcaaggcgct agagactctt gccgtaagat attcagccat
    24121 ccgtatcaca gaagccgagc tagtcgaaat gaaagaggtc ctagacaaga tatatgaaat
    24181 aaggcagagc atgacgggcg atgagcttct cgaaaacctc ttgcctagta tcaaacgctt
    24241 caatgaaata gccttcgaag cttgcaagtc atctagactt accgaagctg tatggtctga
    24301 gagagagatt ttcgaccgct atcgcgtctt gcgcatggtc ttctccaacc gcatagacaa
    24361 aagtatccac cgcagaaatg agctttataa ggccttcctt gcgcatgatc ctgaccttgc
    24421 ttgtcggata tggacagatc atcttaacga gtccttcctg atctggcgcg aaaaatcagg
    24481 caattcggaa gtgttgaagg attttcaatt cttttgaaga tttaaccgac caggccattg
    24541 gccgacggta gatcataaat ttcaagtttt taaggaggag accgaaatga aaaaattgtc
    24601 tctattagtg ctgactgttg ttcttctggg acttgtcgca acagttggcg ctgcacctgc
    24661 ttggaagccg actaagacta tcgagctcgt tgctccggcc aacccgggtg gcggatggga
    24721 tatggtctgt cgcgtaattc agaaagcgct caccgaggaa aaactcgtcg ataagccggt
    24781 aatcattacg aataagccgg gcggtggtgg gtccgttggt tggacttatc taaaaggcaa
    24841 aaaaggccag ggcgaatatc tagccgcgac ctcgagtttg ctcattttaa acaatcttct
    24901 gggcacaagc gatctaacat ataaggactt cactccaatc gcctgcatgc aagcggaatg
    24961 ggaggccatc gtagtgcggg aggactcccc ctggaaaacg gtaaatgatc tcatggaagc
    25021 gataaaagcc aatcctggcg cggttcctat agccgtcggt ccttcgctgg gcaacgacga
    25081 tcaccttgtt ttcctgcagc ttgcaagaac ctatggtgtc aaccccgcaa aagtaaaatt
    25141 catcgtatat ccgggtgctg gcggcgagga agtcccagcc ctacttgggg gccatgtaaa
    25201 agccatggtc attggccttg cggaagtgct cgaacagcat agagccaaaa aactccgcgt
    25261 aatcggagtg agttcgcccg aaagattgga acttctgccc gatgtggcaa cgataaaaga
    25321 acagggaatc gacattgtat ttccacactg gagaggcatt ataggccctc caggattgac
    25381 tcctgaacaa gttgcctatt gggatgaagt gttcacaaag atggtacaga caaaaacctg
    25441 gaaagagcaa ctcgagaact tgctttggga tgattactac cagaattcct cagaccatgc
    25501 caaattccta gaagaacaaa caaaggtttt cgatgagcta ttgaccgcag taggtttgaa
    25561 gaaataatcc aacaattatg aagagccggt gctatgccaa gactcgcatg cgccggcctt
    25621 tttattagac ataatttcta gggcggaggt cttgaatgac aacaaatatt cttacgggaa
    25681 tcatcagcat tgttctaggc ttggtgtatt ccataacggc cttgcgcctg cctcgaatgg
    25741 ctatgggaga caggctaggc cctaaaatat tcccttattt cgtcggtata gttacgataa
    25801 taaccggcat tgccttgatt ttgcaggata gagatccaga aaagcgctcg aagaaagccg
    25861 aatttggttt taaagaacat aaaaatctat ggttaaacat agtcattttg acaattgctg
    25921 gcatcctctt tggatttttc atagatgaat tgggctatat gatagctact gcgttgttca
    25981 tcttctcagt ttcaatgctg ataaacaaag gaaagttggt acaaaacata gtgattgccg
    26041 tcatttttcc cgtggtgact tactttgtat ttggggttgc acttaaactg agccttcctc
    26101 gcggtatcat tgaaacgatg cttccatttt aggagataca cccatgcaat ccattttgac
    26161 aaatctatct atcgggcttc agaccgcatt tacatttaat aatattcttt gggttttcat
    26221 cggaggcaca ttgggcacca taataggcat gctacccggc ttaggacctg caacaggtgt
    26281 agctattcta atacccatta cctttggcat gaacccagcc acggctctca taaccatggc
    26341 agcggtatat tatggagcta tgttcggagg ctccagggca gcgattatgt tgaacatacc
    26401 tggagatgct tccgctattg tctcttgctt cgatggttat ccaatgacca aaaaaggcaa
    26461 agccggtaca gcgcttgcca tctccgcaat cgcttcgttc atcggtggaa cgattggcat
    26521 gatatttctc atttttctta gccaaccagt tgcttccgcg gcgctaaagt ttggaccagc
    26581 ggagatgttc tcgctcatgg cctttgccct ggtggcaacc atcaccttat ccgaagggaa
    26641 ccttttgaaa gggctctttt cgatagtggt agggcttatg ctctcgacca taggaataga
    26701 cccacttgct ggagtcgtgc gttttaccta tggagtttcg agtcttaatg aaggtatcga
    26761 ttttcttgta atcatcatcg gagtctacgc cgtcaccgaa gtatataaaa acttgaaatc
    26821 aatcgataga gtatatgagt ttgattccaa aagcattggc aaggtaaaag tgagcaagga
    26881 agagttcaaa caatgcattg tgcctatgct ccgaagtaca ccactaggat ttataatcgg
    26941 agttcttcct ggcatgggcg gtacaatcgc cacattcatg tcctatgcca tggaaaagaa
    27001 tcttagca
//
LOCUS       DAOMZL010000018        26013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_1567_length_26013_cov_13.232645, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000018 DAOMZL010000000
VERSION     DAOMZL010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 26013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26013
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1567_length_26013_cov_13.232645"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLQ30_02760"
     CDS             <1..299
                     /locus_tag="PLQ30_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW01395.1"
                     /translation="QLISFARVLAHEPSVIILDEATSSIDTETEKLLQRGIEGLLKGH
                     TSIVIAHRLSTIRDANRIIVLGQGRVAEIGTHDELIALHGLYWNLYRLQNKEME"
     gene            300..1112
                     /locus_tag="PLQ30_02765"
     CDS             300..1112
                     /locus_tag="PLQ30_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HPW01396.1"
                     /translation="MMPFSEEKEPFIIGQDDRVTAVYKPCGWHSVCQTKSDHSIVSWL
                     YDQGIPRLRESQMDQELGLIYRLDQMTSGILLFAANPQSLEELRQAQKELAIVKRYIL
                     ISTFSECELLGSLPGTLKEKQSCLIEELFSKREAYIESYFRPYGPKGASVSCIAAEFA
                     SKSKKPITKEIYVSKFIAAYRMIEESNARLRLPNEAVCLEVEIRKGFRHQIRAHCAWI
                     GLPILGDMLYGGQGSNRLWLEAFSVCLPQADRCRNEWKLYNGIQDIAQVSSE"
     gene            1271..1441
                     /locus_tag="PLQ30_02770"
     CDS             1271..1441
                     /locus_tag="PLQ30_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPW01397.1"
                     /translation="MAYRVTEACTNCGSCESECPVSAISEKDNARFIDPETCIDCGAC
                     AAACPTEAIIAG"
     gene            complement(1544..2875)
                     /locus_tag="PLQ30_02775"
     CDS             complement(1544..2875)
                     /locus_tag="PLQ30_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HPW01398.1"
                     /translation="MKKRKIIIIGAAGRDFHNFNARYREDESTEVVAFTAAQIPNIDG
                     RRYPAALAGRLYPEGIPIYSESELSDLIRKYDVDECVFSYSDVPYSHVMKMSAIVNSA
                     GASFALLGPKETQLKSSKPVISVCAVRTGSGKSQTSRKIVQMLMKRGLKVVAIRHPMP
                     YGDLVQQKVQRFATIEDLAHYRCTIEEMEEYEPHISRGNVIYAGVDYEAILRQAEKEA
                     DVILWDGGNNDFSFYIPDLQITVADPLRAGNEVSFYPGEVCLRTAHVIIINKIDSSSP
                     EQIQIVRENIARTNPSATVIEAASPIIVDRPELIRGKRVLCVEDGPTLTHGMMKFGAG
                     VVAARKFQASQLIDPRPFLSGGLTQTFNTYPDIGTLLPAMGYSDMQKRDLEDTINRTD
                     CDTVIIATPIDLNRIIHIKKPTVRVDYELQEIGLPNLEEVLEDFCKRKGLS"
     gene            complement(3156..6662)
                     /locus_tag="PLQ30_02780"
     CDS             complement(3156..6662)
                     /locus_tag="PLQ30_02780"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HPW01399.1"
                     /translation="MKIKRLFTRAGYGPYEKIKWEKRKSEIKNPDGSIVFSTDSVIVP
                     SFWSSIASDILAQKYYRKAGVPKDKALEWTKFVPVSQTILSEEDMVDDAEHDARQVFH
                     RLAYTWCDWGQKAGYFDSEEDRRAFYDEICYMLAHQIAAPNSPQWFNTGLFSVYGIEG
                     PAQGHYFIDPDSNEMAKSESAYERPQPHACFILDVKDDLVNEGGIMDLVTKEARLFKY
                     GSGTGSNFSRLRAANEKLSGGGVSSGLLSFLKIADRSASAIKSGGTTRRAAKMVILDA
                     DHPDVEDFVHWKAEEEYKVACLAAGSGLLNHYAQAMISAIEDMGVQDENSYLPDRNLL
                     LGKAIRQALDAGIPSSWIHQVLTYYAQTGRTPRMPQYNTSWESEAYNTVSGQSSNNSV
                     RVDNDFINAVINDLDWKLIARTTRKPFRVTKARDLWNEIAESAWFCADPGLQFHTTIN
                     EWHTCSADGEIRASNPCSEYMFLDDTACNLASLNLLSFYNFETCKFDEAAYRHTIRIW
                     TLVLEISVVMAQFPSKEIARNSYDYRTLGLGYANLGSLLMVMGLPYDSDNGRALAAAL
                     SAILTGEAYTQSAIMAREFGPFARYEANRQSMLRVIRNHRHAAYDAAKEQYEALSVFP
                     KGLDSRSCPTNLLNAAHECWDEALKLGEKHGFRNAQVTAIAPTGTIGLLMDCDTTGIE
                     PDFALVKFKKLAGGGYFKIINTSVPPALHALGYSDSQIKEIIQYALGKGTFEGAPGIS
                     RQSLIDKGIPNDSIDRIEKSLQNAISLDSCFSPSIVGYDVLEKLGISKEEANAPGFSL
                     LSRLGYDEKQKKEAEIFACGTMGMEGAPHLKSEHLSVFDTATPSGKKGSRSISWQAHI
                     AMMAAVQPFITGAISKTINMPNNVSIDDIKGAYLLAWRSMLKSIAIYRDGSKLSQPLA
                     ALTPGSNLIADSIIALQSDELTSEDISSLEMVPTGLAHKSIEPELPGLPVAPRGVRRA
                     LPNRRKGYTQKAKIGGHSIFLRTGEYEDGSLGEIFLDMHKEGAAFRSILNSFAIAISL
                     GLQYGVPLEEYIDAFTFTKFEPNGIVSGHDYLKMTTSVLDFVFRDLAISYLHRYDLGQ
                     IKPEDLIATSTQSLSAKVPNAFESGKKHIPIKKETDIMLIREAKQKGYEGDACPVCGN
                     LTLIRNGTCLKCETCGSTTGCS"
     gene            7036..7968
                     /locus_tag="PLQ30_02785"
     CDS             7036..7968
                     /locus_tag="PLQ30_02785"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPW01400.1"
                     /translation="MSISDLACSLKATLEYDPLVHAGTITKEGRSVRFAMDVPYVLLD
                     WTIVKEVPAPYESATSLQIKEEFARTVQEFFEIKSQISSRYLVKAIVIDPGHGGKDPG
                     AIGEFEDFKLQEKDVNLSIALRLADLLRLRYPEKTIVLTRSSDIYPTLEERVEKANKI
                     ELEETEAIIYVSIHANASFNKNTKGFEVWYLNPNYRRTVVDERTAKEKGEDIAPIINI
                     MLEEEFTTESIILAQKVYQRMDRIIGDRSPARGIRAEEWFVVRNAKMPSILIEVGFVT
                     NKAEALLLSQAGYLRQIADAIYNGVCDFIEHFEQ"
     gene            7970..8422
                     /locus_tag="PLQ30_02790"
     CDS             7970..8422
                     /locus_tag="PLQ30_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01401.1"
                     /translation="MNPSKEQDHKQGYIIALVIFLAIVFIVWKGPTLQYNSMRLYFPD
                     KKKGELRLEKRPIAPIGTLKEKAKATVEELLLGPLSRDLKILTDTDISLLRIVEGKNA
                     LFMDFSTQDIASFSSEYMTFKSALEKSLKETIPGTFHIYFFINGVLAR"
     gene            8583..9881
                     /locus_tag="PLQ30_02795"
     CDS             8583..9881
                     /locus_tag="PLQ30_02795"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW01402.1"
                     /translation="MNDLENIPNKTLLEKLYNQHRTGYEEALAKTVNDISNLLQDKGI
                     HPTIKSRVKDFESYFSKKIRFLKKAWDEHKDPLPINDVLAMRIICPFLRDLEETVHLL
                     DQRYEVVEIERKGHDRSVREFGYESIHVLIRIPDELVPLCEGLERYVMEIQLRTILQE
                     AWAEVEHEIVYKAEFTPLDEPLKRKLAALNATLTLSDIIFQEILDYQKRLSDALKKRR
                     QGFYGKIEQKTAEIFGISEEITEPIPSFSGQMMEQRETAYPNENQVSASNRSNGLDMD
                     MLLLSALNAHNASDFNSAISIYTKILDKKPEASLAAVVYKHRGMAYFAQSEYEQALAD
                     FTSCLILDPNCYKALYYRGVVKSIQKELPQAIEDFSKALEIHPYHFFSRYRRALCWWQ
                     LGDYAQAHADCELALRIMPENKLAQELQKKIMEHLAKEDI"
     gene            10014..10085
                     /locus_tag="PLQ30_02800"
     tRNA            10014..10085
                     /locus_tag="PLQ30_02800"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10047..10049,aa:Glu,seq:ttc)
     gene            complement(10123..11310)
                     /gene="thiI"
                     /locus_tag="PLQ30_02805"
     CDS             complement(10123..11310)
                     /gene="thiI"
                     /locus_tag="PLQ30_02805"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HPW01403.1"
                     /translation="MHDLFLIKPGEIELKLGNRREFTRRLKEQITKRLKGIPFIFEEY
                     PGRFFLTVEEKDSELALFVLQHCPGINGVAKAIRIKKDIDEICQATVECAKTEISAGS
                     HSFKIEARRSDKSFPLDSYKICVVAGNSLLSEYPKLSVDVHNPDFIIRIEIREQAYVY
                     SRTVAGPRGLPVGSQGKGVLLLSGGIDSPVAGYMMACRGLALESIYFHSYPYTSPEAQ
                     QKVERLAARISIWTGGMNLWIVPFTDIQLAIAKASYEEAKTLMLRMAMMQVADIIAHR
                     INANSVVTGESLGQVASQTAENMRLSQSKTSLPVLRPLIGIDKEGIIAIARQIGTYDI
                     SILPYEDCCVLFSPKHPILKPDFGELRSHFESLALGNLIESAVDNSERKCFDFADALK
                     SGI"
     gene            complement(11324..12505)
                     /locus_tag="PLQ30_02810"
     CDS             complement(11324..12505)
                     /locus_tag="PLQ30_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPW01404.1"
                     /translation="MKDIYLDWAATAPPDPDIMRIALEFSLKAYGNPSSKYWLGKEAR
                     QALEESRLRVMRSMGVTKGSLIFTSGGTEADHIPIFAILEKLKRASSAEKPMHAIISS
                     IEHSAIEEQMRILERLGLQVSRIDPDSKGHIEAEQIESRIRKETALICIMNVNNETGA
                     IQNIKAIAESIKETVLQKKIKKPWLHVDAVQALGKLSIRDLEDYADSFAISAHKIRGP
                     KGVGGLWIARPLEAFIRGGGQESGMRPGTENLFGALAFSLAAEKAALSMQENQEKAIA
                     LEKRLLDGISSIPNTLTLPERIPQDKGFVPSIISMAFPGLGGETMVRALSDCHIAVST
                     GSACMSNIHLAERRVLKAMLVPDDIAFSSIRVSTGPLTTPQDIDSFLEQAEVLYRRLK
                     T"
     gene            complement(12513..12815)
                     /locus_tag="PLQ30_02815"
     CDS             complement(12513..12815)
                     /locus_tag="PLQ30_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HPW01405.1"
                     /translation="MNLENISIMKKEGMRKDHEIMVYALSTCIFCKRALTFLDVNHFA
                     YKYLYIDLVPLESKNAIKNELTQRFKENVIFPFMVIDSREHLIGFIETEWKMALGA"
     gene            12943..14757
                     /gene="lepA"
                     /locus_tag="PLQ30_02820"
     CDS             12943..14757
                     /gene="lepA"
                     /locus_tag="PLQ30_02820"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPW01406.1"
                     /translation="MIEPAFIRNFCIIAHIDHGKSTLADRFIEKAHLIDSRKFQDQML
                     DNMDIERERGITIKSQAISLPYVAKNGKTYMLNLVDTPGHVDFSYEVSRAIAACEGAV
                     LVIDATQGVEAQTLSNMYMALEHDLEIIPVINKIDMASADIEGVRGQITKDLGLEGES
                     ALAVSAKLGIGIDELCEAIVERIPSPKGGAENPLQALIFDSHYDPYRGAIVHLRLKEG
                     SIKKGMHIRLMSTGAVHEVEEVGHFRMALSPAMELSAGEVGYCIAGIKTVKEVRVGDT
                     LTDHENPCNTALPGFREVKPVVFSSIYPMDSSDYEELRDSLEKLVLNDASLIFEKDHS
                     LALGFGFRCGFLGLLHLEIVQERLEREYDQSIIMTAPSVRYKVILKDGSTLYIDNPSD
                     YPDPSRIENVEEPYIKAEIITPVDYIGNIISLCVSKRGSQQSFLHLDERRVELIFEMP
                     LAEVLFDFYDKLKSISRGYASFDYVVTGYRPTDLVKLDFLINGEPVDALSMLVYRPNA
                     YDRARVVCERLRNNIPRQQFKIPIQGSIGSQIIARETVNPVRKDVLAKCYGGDITRKR
                     KLLEKQKEGKKKLKIIGNVELGKEVFLSVLRSSEEEDR"
     gene            complement(14821..16425)
                     /locus_tag="PLQ30_02825"
     CDS             complement(14821..16425)
                     /locus_tag="PLQ30_02825"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPW01407.1"
                     /translation="MKGQRMASEENEKQAKTGIKKSGDKKGDNKQTKTTLKKTIKNPF
                     VYIGTIIILVLTIIAFVLIPSISSGTGAGAKLPSFGSWNGNPIQYTSGSYFAQQVSQI
                     NDYLRQQGLDETQSQLYAYQVWRLAFQNTVIRTAILDSAKRSGMKVSEETIDEEISKN
                     SQFQVDGKFSLEKYNSTPAATRLSIRNSIREDMLIQNYYNDLMSASPSTAEIEFVASM
                     AKPQRAIQYVNISYADFPVEETLEWAKSHESLFRTIGLSRITISTSEKDAKKVLSQIK
                     ENSLSFEDAAKSHSKDVYADKGGDMGTKTFYDLKGEFLDEKDAENLMSLKKGEISPVY
                     KQTGNTWAFYRINSEAKAPDFSNQSVLDEVKSYIFDREKSVLESWALAKANEFKAELM
                     NESFESAAKKVGLQVKPAGPFILNYGNPGFYFYGQQVSLFQEPYRSLDTDLVGAVENE
                     TFLTTLFSTPKDAVSEPVLLDASVVVMKVTEDREASDQETSYTKFSYPYFFQTAMENH
                     IRNSILTSDKFKDNFNATFAKLFVSN"
     gene            complement(16466..17392)
                     /locus_tag="PLQ30_02830"
     CDS             complement(16466..17392)
                     /locus_tag="PLQ30_02830"
                     /inference="COORDINATES: protein motif:HMM:NF016277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01408.1"
                     /translation="MTSENRYVLYFQVKGTCPYLSNNHSTMMNLLIFQDEVDSRLYSA
                     LISNGWRRHGKEIYRMECPGCKLCIPLRIKAESIQLTLSLNRILKKNNDIIIISNPPE
                     FDEEHFRLWQRYSIWKHSIPLKDLKEDSYKNLLEPWSLVFEYRDNSKGRKLLAVSHVD
                     PLDDGLSSVYFSFEPDARGRSLGFFSILAESYLSSFAKKAKHLLLNKEIKGESVSHFY
                     ENRIQHKLGRDDAKSQAADNDSEDKRYYYLGFWVPGASKMDYKARISPFEIAIADENI
                     DDPNPWKRFLSEKEAIAYLSKQKWPGLSLTIP"
     gene            17498..18019
                     /locus_tag="PLQ30_02835"
     CDS             17498..18019
                     /locus_tag="PLQ30_02835"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPW01409.1"
                     /translation="MSIITKTGDEGTTGLWSGERIGKDSPRVEAYGTIDELSSALGMA
                     RHLCMQDNVLCAIEYIQRLLFRVAGELASMSMPFDNPINEDDEKTIEDKTNELEMRIP
                     IRSFVMPGMTGGSAALDLARTIARRAERRVVALSRIEHVSPVLLRILNRLSDYIYMLA
                     REEESKQGKLTFI"
     gene            18096..19367
                     /locus_tag="PLQ30_02840"
     CDS             18096..19367
                     /locus_tag="PLQ30_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPW01410.1"
                     /translation="MRLYSKAQIGLVIVVSVTLTALSIAGIVFVLDRSDQNQSTMAVA
                     SKIAQDGDEAVYIPEITSLGIQDDKTASLSLQVANSTLSMSSYSADEIENITIYEKYN
                     ESVVNINTEVLGINWFLEPVPQSGGSGSGSIIDEQGYILTNNHVVEGAYKLYVSLADG
                     SRYEAKLIGTDPESDLAVIKISPEPGKKLKPIPFGTSKDLKVGQKVLAIGNPFGLERT
                     LTAGIISGLGRPIQESETTILQNMIQTDASINPGNSGGPLFNMKGEMIGINTMIYSPS
                     GGSVGIGFAIPVDTAVRIAPQLIKNGQVKRGWIEMQVIQLFPDLVNYLRQSGKAAPVE
                     TGLLVSTVTSGGNAEKAGLKGGTTAVRYRSTVFYIGGDIIVSIDGKPVTKIAELYAAL
                     EDTSPGQRVNVEFYRGSKRMSTVVILAERRR"
     gene            19364..21343
                     /gene="uvrB"
                     /locus_tag="PLQ30_02845"
     CDS             19364..21343
                     /gene="uvrB"
                     /locus_tag="PLQ30_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPW01411.1"
                     /translation="MKPFKVVSPFEPAGDQAQAIETISSAIKRGEKYLTLKGVTGSGK
                     TYTMAKVIEAIQKPALVISHNKTLSAQLYREFKEFFPENAVEYFVSYYDYYQPEAYVP
                     SKDLYIEKDSDINEEIDRMRLSATASLMERRDVVIVATVSCIYGLGSPDLYREMRLYL
                     DKGQIVDIDEIKRNLISLQYERNDMVLERGRFRIRGDVLEIFPAYLEEAYRIELDFDT
                     IVRIRRLDPLTGEASNELEEAIIYPAKHFVMPEDLVQKAVDSIRNELEERYQFFNAHG
                     KYLEAERIKSRTEYDIEMLSEMGYCSGIENYSAPLSNRKPGERPGVLLDYFPKDFICF
                     IDESHVTLPQLGAMYNGDHSRKLSLVDYGFRLPCALDNRPLRIDEFESIVAQTIYVSA
                     TPGETELRRSKTLAEQVIRPTGLVDPEILVHPTEGQMEDIYARVRERARQGERSLIIT
                     LTKKMAEELTDYLSELGLRVRYIHSEVETIERVEILTQLRAGEFDVLVGINLLREGID
                     LPEVSFIAILDADKIGFLRSETSLIQIIGRAARNAAGLVVMYADVMSPAMKNAIMETE
                     RRRKMQQAYNLEHGITPRTISKKVQDILERHKQERIEASHGEIEMLRRTHNLLVPEQR
                     RSLIKELEKLMFEHAKNLEFEQAALIRDEISKLKE"
     gene            21358..22359
                     /locus_tag="PLQ30_02850"
     CDS             21358..22359
                     /locus_tag="PLQ30_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPW01412.1"
                     /translation="MSNNHNQEDFYVIDAHCDTLMAIVGRSMNKEERDPRNFFADNNS
                     VHLDLPKLSRGHVACQVMAIFLEDSQLSEATREAMAMIDSLESLLRFDPREFFLARNS
                     ADIYRALETKSVCALLSIEGAEALGDSLESLEIFYQRGVRAIGLTWNRRNAFGRGLKA
                     EGSDGLTNLGKELVEKMEEIHVIIDVAHLGEEGFWDVVDISTGPFIASHANARDILDI
                     PRNLSDSQIRAIADHGGAIGCVFVPGFVTKDPGDCSLEAFLLHIDHIVRVGGIESCAI
                     GSDFDGFMPQGGKVIQDSGEFPLLYEALRSHGYDHAAAQKILGGNWLRIFDDVLGLV"
     gene            22356..22898
                     /locus_tag="PLQ30_02855"
     CDS             22356..22898
                     /locus_tag="PLQ30_02855"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPW01413.1"
                     /translation="MKKIALIGMMGSGKSEIGSLLASNYGIDFIDMDRAIEEHTKTTI
                     PTIFRDFGEEHFRDLEQNQLALMAVSFSSLVLSCGGGIVLRDSNRLILRDSFLTIWLD
                     VSITELKRRLQNQRHRRPLLLSDEWESQIEEIYRQRYELYRSTARIQYTWKDNHSMEE
                     SAIDIKRLIDAAYNGISGRT"
     gene            complement(22871..23965)
                     /locus_tag="PLQ30_02860"
     CDS             complement(22871..23965)
                     /locus_tag="PLQ30_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW01414.1"
                     /translation="MSLEVNNLLIERDSFALFADFSLEEGKILALLGPSGCGKTTLLR
                     SIAGLEMIKEGTITLNGIRIDRLPTEKRHIGFVFQDLALFEQLRVRDNIGYSLFIRSE
                     EKRKREERVISLARNFKIEHLLGRYPFELSGGERQRVAFARAFASDPLLMLLDEPLSS
                     LDAPLRREMRRFIREQLSKQCLTTIHVTHDVEEAIDLGDEIIVMRDGKMISRGIINDF
                     EKKPGSGWLARFMNLGLVLPITEISASKESQLLNIMTSEGNTFQVSRNSVSNIDKALE
                     KAMCLYIPFSAIEIFGKNNNRKTMKVKIVRLISATPLASKLVLTIPEMQDYFFELPTP
                     ISDFSMSSQGKEIEISIDQGMCQVLPEIPL"
     gene            complement(23955..25616)
                     /locus_tag="PLQ30_02865"
     CDS             complement(23955..25616)
                     /locus_tag="PLQ30_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPW01415.1"
                     /translation="MRAKIFLYLVLCIILIFSLAPLIAFLHAAIFGLSEIKGNIGGIE
                     IILDFLKQIPESTIAKSLWFTIRQSFASTALAISLGLPGAWLAARFRFPGRKTLLSLA
                     AIPFCFPPILIILAFICYYGKEGLLPTALSLVFGSRGEYRGILYSFWGLVIVHAFYNF
                     PIVVHQISALLLRIPNTSFEAARTLGARKSKAFRTGILPWILPGIGQSAGLIFLYCFF
                     SFTTVLVFGGRAGTTLEVEIYQALRYRSNLALAAVLSIIESACALLGIWMLTRIQSKT
                     RRTVRDFGAAHKLAKPKGKQWFLIVLYEILIIVFFIGPLFSIVIEAFTVPASLNSPER
                     FGVDNFKRLFTGFQAPLTGAILSTLKLSGIAALLATSIGILASFSTFLAQRIGKKSML
                     IDLMMDLLQWLPMAVSPAVLAYGWLFISANHIATYALIAAQALIAWPFVSRTVGASLR
                     AIDFRIHEAAKTLGASPLRAFMTIELRTILPSVAAAAAFAFSITAGDVNVPLMLGMSE
                     IETLPLLLYRLTAAYRFNEACAAGLVLGLMTGIVFMLKEKAVDVA"
     gene            complement(25613..>26013)
                     /locus_tag="PLQ30_02870"
     CDS             complement(25613..>26013)
                     /locus_tag="PLQ30_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HPW01416.1"
                     /translation="TPSWDTGYGLFTKGEAPLVLSYSTSPAYHKAFENSERYQALQFV
                     EGHPTQIEFAGVLISSKKRELAQEFIDYLLSAKCQALLPETQWMYPANIKTELPPSFS
                     IVQKYPALSATIRDLDHDPSEAADLLIGNP"
BASE COUNT         7316 a         5510 c         5898 g         7289 t
ORIGIN      
        1 gacaactaat ctcttttgca agagtccttg cccatgagcc atctgtgatt attctggatg
       61 aggccacaag ttctatcgat accgagacag aaaagctact tcagagaggc attgaaggtc
      121 ttctaaaagg ccatacaagt atcgttatcg cacataggct ttcaaccatt cgcgatgcta
      181 ataggatcat cgtacttgga caaggtcgtg tggcagagat aggcacacat gatgagttga
      241 ttgccctgca tggtttgtat tggaatctct ataggcttca aaacaaggaa atggaataaa
      301 tgatgccatt ttcagaagag aaggagcctt ttataatcgg ccaagatgat cgggttacgg
      361 ctgtttacaa gccatgcggc tggcattccg tatgtcaaac gaaaagcgat cactctatag
      421 tatcttggct atatgatcag ggaataccta ggcttagaga gtctcaaatg gatcaagagc
      481 tcggtcttat ctataggcta gatcagatga catctggaat tctgctattt gcagcaaatc
      541 cgcagagtct cgaggagctt cggcaagcac agaaagaact tgccattgtg aaacgctata
      601 ttctaatctc tacattctca gaatgtgagc ttttaggatc tttgcctgga acattgaaag
      661 aaaagcaatc atgtttgatt gaagagcttt tttctaaaag agaagcatat atcgaaagct
      721 attttcgtcc atatggtcca aaaggcgctt cggtgtcctg tattgcggca gaatttgctt
      781 ccaaatccaa aaagccaatt acaaaagaga tatatgttag taaatttatt gcagcctata
      841 ggatgataga agaaagcaat gcaagactgc gactaccaaa tgaggcagtg tgcctcgagg
      901 ttgaaattcg gaagggtttt cgccatcaaa ttcgggctca ttgcgcatgg ataggcttgc
      961 ctattcttgg agatatgcta tatggcggac aaggctccaa tagattgtgg ctcgaggctt
     1021 tttcggtatg tcttcctcag gctgatcgat gccgcaatga atggaagcta tataatggaa
     1081 tccaagatat tgcccaggtt tcatctgaat gaagattcct gttctatata ttattccaca
     1141 atgatttttc taacagcaaa ttgcgaacaa gtctggacac aatccaaaac ccattcccat
     1201 attcgatgct tgatgataaa ataataaaac atccttcaag gaatgatatt actttttaga
     1261 gaggtttgaa atggcgtaca gagtgacgga agcttgcacg aattgtgggt catgcgaaag
     1321 cgaatgcccg gtcagcgcaa ttagcgaaaa ggacaatgct cgatttattg acccagagac
     1381 atgcattgat tgtggagcat gtgccgcggc ctgtccgaca gaagccatta ttgctggcta
     1441 aggtccaaga ccataagagg ggctgcccaa cgagttgggc agcccctctt gttttcatgt
     1501 aggatcaact cattaccttg aggagattct tattatattt atattaagat aagccttttc
     1561 tcttgcaaaa atcctctaac acttcctcga gattaggcaa tcctatctct tgaagttcgt
     1621 aatcgactcg cacggttggc ttttttatgt gaataattcg gttcaaatca ataggagttg
     1681 ctatgatgac agtgtcgcag tcggttctat ttatggtatc ttcaagatct cgcttctgca
     1741 tatcgctata tcccattgct ggaaggagag tccctatatc gggataagta ttgaatgtct
     1801 gtgttaatcc cccactgagg aatggccggg gatctatgag ctgggaggct tggaattttc
     1861 gagctgctac cacaccagct ccaaatttca tcatgccatg tgtaagagtg ggcccatctt
     1921 ccacacacaa tactcgcttg cctcggatga gctcggggcg atccacaatg atagggctag
     1981 ccgcttcgat tacagtggcc gaaggatttg tccgtgctat attttctctc actatctgga
     2041 tctgctcggg tgagcttgaa tcgattttat tgattattat gacgtgagct gttcttagac
     2101 agacttcgcc agggtaaaag gatacttcat tgccagcgcg cagcggatct gcgaccgtta
     2161 tttgaagatc tggaatatag aacgaaaagt cattatttcc gccatcccag agaattacgt
     2221 ctgcttcttt ctccgcctgg cgaagaatgg cctcgtagtc cactcctgca tagatgacat
     2281 tgccacgaga tatgtgcggc tcatattcct ccatctcttc aatagtacac ctataatggg
     2341 cgagatcttc tatagttgca aaacgctgga ctttctgttg tacaagatcc ccataaggca
     2401 tgggatggcg aatcgcaacc actttaagcc cgcgcttcat aagcatttgg acgatttttc
     2461 gcgaagtctg acttttgcct gagccggtgc gtactgcaca aaccgatatg acgggcttgc
     2521 tcgattttag ttgggtttcc ttagggccaa gcagcgcaaa ggaagcaccg gccgaattca
     2581 caatcgccga cattttcatg acatgagagt agggcacatc ggaataagag aatacgcatt
     2641 catctacatc atattttcta attagatctg aaagctcgct ttcggaataa atgggtatcc
     2701 cttctgggta aagtcgtcct gcaagagcag ctggatatct tcttccgtcg atatttggaa
     2761 tctgcgcagc cgtaaaagct acaacctcgg ttgattcatc ttctcgatat cgagcattaa
     2821 agttgtgaaa atcccttcct gctgccccga tgattattat cttgcgcttt ttcatcaaag
     2881 ctccttcctt ttgttttgcc ttatgaatga caaagacatg cattcatttt tgcctatttt
     2941 cgtatcagag tcaataaggt cacattataa taacttttta taatataagc attaaaatga
     3001 ataatgatgc gatcttgaag ctattttatt gagaattttg caagatttgc caaataataa
     3061 ctaatacata tacagtctaa ggagggttag tgacggtcat ggcaatcttt atcaggccgc
     3121 tgtcataatc cataatatcg acttatactt aagagtcagc tgcatccggt ggtggatcca
     3181 catgtctcac atttgaggca ggtaccattt ctgatgagcg taaggttacc acaaacagga
     3241 caggcatctc cttcataccc tttttgcttg gcttccctga tgagcataat atccgtctct
     3301 ttttttattg gaatgtgttt tttaccactc tcaaaggcgt taggaacctt agcggaaagc
     3361 gattgcgtgg atgtagcaat gaggtcttcg ggcttgatct gtccgagatc ataacgatga
     3421 agataggaga ttgcgagatc gcggaaaaca aaatccagga cacttgtcgt catttttaga
     3481 taatcatgcc cgctgactat tccgtttggc tcgaatttcg taaatgtaaa ggcgtctatg
     3541 tattcttcaa gaggtacccc atattgcaaa cccaaggata tggcaattgc aaaagaattc
     3601 aaaatgctcc tgaaagcagc accttcctta tgcatatcga ggaaaatttc cccaaggcta
     3661 ccatcttcgt actcgccggt tctaaggaag atggaatgac cccctatctt tgctttctgg
     3721 gtatagccct ttcttctatt tggaagagct ctcctgacac cgcgaggtgc aacaggaagg
     3781 ccaggaagtt caggctcgat actcttatga gctaagccgg ttggaaccat ttccagagaa
     3841 gaaatatctt ctgaagtgag ttcatcagac tgtagagcta tgatcgaatc ggcaatgaga
     3901 tttgatcctg gtgttaaagc ggcaagaggt tgagaaagtt ttgatccgtc tctatatatg
     3961 gcgatggatt tcagcataga tctccaagcg agcaaatatg ccccttttat gtcatctatg
     4021 ctcacattat tgggcatgtt gatggtcttg gagatggctc ccgtgatgaa tggctgcact
     4081 gctgccatca tggctatatg cgcttgccac gatatgctgc ggctgccttt ctttccagag
     4141 ggcgttgccg tgtcgaagac agataggtgt tctgatttaa gatgaggggc gccttccata
     4201 cccattgttc cacacgcaaa gatctcagct tctttttttt gcttttcatc atagcctaag
     4261 cgagatagaa ggctgaatcc aggagcatta gcttcctctt tcgatattcc cagtttttct
     4321 agaacatcat agccaacaat agatggagaa aagcagctat ccagactgat tgcgttttga
     4381 agagactttt cgattctatc gattgagtca tttggaatgc ccttatcgat tagtgactga
     4441 cgggagattc ccggtgctcc ttcgaatgtt ccctttccca gagcatattg tatgatctcc
     4501 ttaatttggc tatcagaata gccaagcgca tggagagcag gagggaccga ggtgttgatg
     4561 atcttgaaat accctccgcc agcaagtttt ttaaatttga caagcgcaaa atcgggttcg
     4621 atacctgtag tatcgcaatc cattaggagt ccgatagtgc cggttggtgc tatggcggtc
     4681 acttgagcat tcctaaaccc atgcttttct cctagcttca atgcttcatc ccaacattcg
     4741 tgcgctgcgt ttagaagatt agtagggcat gaccgtgaat ctaggccctt agggaaaaca
     4801 ctcaatgcct cgtattgctc ctttgcggca tcatatgccg catgcctatg atttctgatc
     4861 actctaagca tagattgtct attagcttca taacgagcaa agggcccaaa ttctctcgcc
     4921 ataatagctg attgggtata agcttcacca gtaagaattg ccgatagagc agctgccaga
     4981 gctcttccat tatcggaatc ataaggaaga cccataacca tgagcaggct tcctagattg
     5041 gcgtatccca atccaagggt ccgatagtca taactatttc tagcgatctc cttagaggga
     5101 aattgcgcca ttacaaccga aatttcgagt acaagtgtcc aaatccgaat cgtatggcga
     5161 taggctgctt catcaaattt gcatgtttcg aaattataaa aagatagcaa attcaaagaa
     5221 gccagattgc aggctgtatc atcgaggaac atatactcag agcatggatt agacgcgcga
     5281 atctcgccat cggcggagca agtatgccat tcattgattg tcgtatggaa ttgcagacca
     5341 ggatccgcac aaaaccacgc tgattctgca atttcattcc agagatcgcg cgctttggtt
     5401 acacggaagg gcttccgcgt agtacgggct atcagtttcc aatctagatc gttgataact
     5461 gcattgataa aatcgttatc aactcttacg gaattattag aagattggcc ggaaacagtg
     5521 ttataagctt ctgattccca ggatgtattg tattgtggca ttcgaggagt tctaccggtc
     5581 tgtgcataat aggtcagcac ctgatgaatc catgatgaag ggattcctgc atcgagcgct
     5641 tgtcttattg ccttacccaa gagcagattt cgatcgggca gatagctgtt ttcgtcctga
     5701 actcccatgt cttcaatcgc agagatcatt gcctgcgcat aatgattcaa aaggccactg
     5761 cctgccgcaa ggcaggcgac cttatattcc tcttcggcct tccagtgaac aaaatcttca
     5821 acatcggggt ggtctgcatc gaggataacc attttagcgg cgcgtctagt tgtaccaccg
     5881 gatttaatgg ctgaggcaga tctatcggct atttttagaa acgagagaag acccgatgaa
     5941 acaccccctc cagaaagctt ctcatttgca gctcttaagc gtgagaaatt tgatcctgta
     6001 ccagaaccat atttgaataa acgagcctct tttgttacga ggtccataat tccgccttca
     6061 ttcaccaagt catcctttac atcgagaatg aaacaagcat gaggctgagg tcgctcatag
     6121 gctgattcgg attttgccat ctcattggaa tcgggatcta taaagtaatg cccctgggcg
     6181 ggtccttcga tgccataaac agaaaataac cccgtattga accattgggg actatttggt
     6241 gccgcaatct gatgtgctag catatagcag atctcatcat aaaaggctcg tcgatcttct
     6301 tcggaatcaa agtatcccgc tttttgtccc cagtcgcacc atgtataggc aagcctatgg
     6361 aatacctgcc gagcatcgtg ttcggcatca tcgaccatat cctcttcaga gaggattgtc
     6421 tgagagacag ggacaaattt cgtccattcg agtgccttat ctttggggac tccggctttt
     6481 cgatagtatt tttgggctag gatatcgctt gctatagagc tccagaatga aggcactatg
     6541 acagaatccg ttgaaaacac aatagaacca tcgggattct tgatctcact tttcctcttt
     6601 tcccatttga tcttctcata agggccatag cctgctctgg taaacaagcg ctttattttc
     6661 atattctctc tccttgagct gcgctatact atctatacta tcatagcagt atgatactac
     6721 aatatatagt ggtgctcttt cttatcttat ttcttatcat ggagacttcc gatgcaataa
     6781 tccctgtagt gattttctgt gtttctctat gtttggtttg ctccagcgca tcggaaaaca
     6841 ctgctttggg gtgacagaca agcctattcc tgctattgtt atgctgaaat gcataacctt
     6901 ccggatatga tcttagtctt aatctccagg aaaagatttt gcttaaagat tttgtacaag
     6961 actctggcca tgctagccat gattgcccta tgcattctac cattatttgg tgcaaatgat
     7021 acaaaacaag aagatatgag catttctgat ctagcctgtt cgctaaaggc taccctggaa
     7081 tatgatcctt tagttcatgc cggaaccatt acaaaagaag gtcgcagtgt gcgctttgcc
     7141 atggatgttc catacgtgct tctcgactgg acaatagtaa aagaagtgcc cgcgccttac
     7201 gaatcggcta catctctgca aatcaaggag gaatttgcac ggacagttca agaattcttc
     7261 gaaataaaat cgcaaatatc atcaagatat ttggtaaaag ccattgtcat agatccaggg
     7321 catggtggca aagatccagg ggctatagga gaattcgagg acttcaagct tcaagaaaaa
     7381 gatgtaaatc tcagtatcgc gcttagactc gccgatctat tgcgccttcg atatcctgaa
     7441 aagacgattg ttcttacccg ctcgagcgac atttatccaa ctcttgagga acgggtcgaa
     7501 aaagcaaata agatagaatt ggaagaaact gaggctataa tctatgtttc catccatgcc
     7561 aatgcctctt ttaacaagaa tacgaaaggt tttgaggtct ggtacttaaa tcccaattac
     7621 cgacgtaccg tggtcgatga aagaaccgct aaagagaaag gagaggacat cgctcccatc
     7681 ataaatatca tgcttgaaga agaatttact accgagagca taatcctagc tcagaaagta
     7741 tatcaaagga tggacaggat aatcggtgat agaagccccg cgcgagggat ccgcgctgaa
     7801 gagtggtttg tagtgcgaaa tgcaaaaatg ccttcgattt tgattgaagt gggttttgtg
     7861 accaataagg ctgaggctct tcttttgtcc caagctggct acttgagaca aattgcggat
     7921 gccatataca atggtgtttg tgactttatc gaacatttcg agcaatgata tgaatccatc
     7981 taaagaacaa gaccataagc aaggctatat tatcgccctg gtgatttttc ttgccattgt
     8041 attcatcgta tggaaagggc caacgcttca atataatagc atgcgtctct atttcccaga
     8101 taagaaaaaa ggagaattga ggctggagaa gcgtcctata gcgccaattg ggactctaaa
     8161 agaaaaagcc aaggctactg ttgaagagct tctgcttgga cctctttcca gggatcttaa
     8221 gatcctgaca gatacggata tttccctgct gcgaatagtt gaagggaaga acgctctgtt
     8281 tatggatttc tcaacacaag atattgcaag tttctcttca gagtatatga catttaagag
     8341 cgctctggaa aagagtctta aagagacaat accaggtact tttcatatct atttcttcat
     8401 aaatggtgtt cttgcgcgat gaattaattt ttgccgatct ctgaataaat cgatgtggct
     8461 gtgcacaatg ctattctctg cggctccatg cacagcacag cacaggcaag ccaggaattt
     8521 aaacaacaat gatgttgatc cctcctttgc cgaaagaaat atttcatgat aactttattc
     8581 tcatgaatga tcttgagaat attccaaaca agactctttt ggaaaagctt tacaatcaac
     8641 atcggactgg ctatgaggaa gctctagcca agacagtcaa tgacatctcc aatcttttgc
     8701 aggacaaagg aatacaccca actataaaga gcagggtcaa agatttcgaa tcctattttt
     8761 cgaaaaaaat aagattcctt aagaaagctt gggatgagca taaagacccc ttgcccataa
     8821 atgatgttct tgctatgcga attatttgtc catttttgcg agatttggaa gagaccgtac
     8881 atcttcttga ccagcgctat gaggttgtcg aaatcgagcg caaaggacat gaccgttcgg
     8941 ttcgcgaatt cgggtatgaa tcaatccatg tgttgattcg cattcccgat gagcttgttc
     9001 cgctctgtga aggcttggaa agatatgtaa tggagattca gttgagaacc attctacagg
     9061 aggcgtgggc ggaggtagag catgaaattg tctacaaggc agaattcacc cctctcgatg
     9121 agcctttgaa aaggaaacta gccgctctaa acgctacact taccctgtcc gacataattt
     9181 ttcaggaaat actggattat caaaaaagac tgagcgatgc gcttaaaaag cgtcgccagg
     9241 gattctatgg aaaaatagaa cagaaaacag ccgaaatctt tggtatatct gaagaaataa
     9301 cagaacctat tccctcattt tctggccaga tgatggaaca aagagagaca gcttatccaa
     9361 atgaaaacca ggtctcagct tcgaatcgat cgaatggcct tgacatggat atgctgcttc
     9421 tctctgccct caacgcacat aatgcctcag atttcaatag tgccatatca atatatacca
     9481 agattctcga taaaaagccc gaggcatcgc ttgcagcagt ggtgtacaaa catcgaggca
     9541 tggcctattt tgcacaatcc gaatatgaac aagctctagc cgattttacc agctgtctca
     9601 ttctcgatcc caactgctat aaggctctat actaccgcgg agttgtaaag agcattcaaa
     9661 aggagctgcc acaagcaata gaagattttt ctaaggctct tgagatacac ccgtatcatt
     9721 tcttttcccg atatagaaga gctctatgtt ggtggcagct tggcgattat gcgcaagctc
     9781 atgccgattg cgaactggca ttgcgcataa tgcccgaaaa caagctagct caggaattac
     9841 agaagaaaat catggagcat cttgctaaag aagacatata aactctcatt gaatgcaagt
     9901 ttgccctcta atagcatctc aattatagcc ttgcttattg acatgtattg gaaattctga
     9961 taggttcact gccgttcggc actctcgccc cgagagaggc cctatgtcgg aatgtcccca
    10021 tcgtctagag gttaggacag cgggttttca acccgccaac aggggttcga ttccccttgg
    10081 ggatatagat ggagccatca tagattcatg gtggcttttt tattatatac cagattttag
    10141 cgcatctgcg aaatcaaagc attttctttc agaattatcg acagccgatt caattagatt
    10201 acctaaagca agcgattcaa aatggctcct cagctcgcca aaatcaggct taagaattgg
    10261 gtgcttaggg ctaaatagga cacagcagtc ttcataaggc aagattgaga tatcatatgt
    10321 gcctatttgt cgagcgatag caatgatccc ttccttatcg attccgatca gaggtctcaa
    10381 aacaggcagg gaagttttgc tctgagataa gcgcatgttt tccgctgttt ggcttgcaac
    10441 ttgcccaagg ctttcaccgg ttacgacaga gtttgcgttt atgcgatggg caattatatc
    10501 ggctacttgc atcattgcca tccgaagcat caatgttttt gcctcctcat aagatgcttt
    10561 agcgatagca agctgaatat ccgtaaatgg tacaatccat agattcatgc ctcctgtcca
    10621 aattgagatg cgcgcggcaa ggcgttcgac tttttgctgg gcttcgggag aggtataagg
    10681 atagctatgg aaataaatgc tctcgagtgc aagaccccga caagccatca tgtatcctgc
    10741 taccggtgaa tcaattcctc cggagagaag cagaacaccc ttgccttggc tccctacagg
    10801 taatcctcta ggtcctgcaa cggtcctgga atagacatag gcttgctctc ggatttcaat
    10861 cctgattata aaatctgggt tatgaacatc tacagagagc ttcggatatt cggagagcag
    10921 gctattaccg gcgacaacgc atatcttgta ggaatccaaa gggaaactct tgtctgaacg
    10981 tctggcttct attttgaaac tatgcgaacc agccgaaatc tctgtctttg cacattcgac
    11041 tgtggcctgg cagatctcat ctatatcctt ttttatccta atggcttttg caacaccatt
    11101 tatccccggg caatgctgaa gcacgaatag agccaattcc gaatctttct cttccacagt
    11161 caggaaaaac ctgcctggat attcctcgaa aataaatgga attcccttga gcctcttggt
    11221 tatctgttct ttcaagcggc gcgtaaattc gcgtctgttg cccaatttta gttcgatttc
    11281 gcccggttta atcaggaaaa gatcatgcat agcccctgta ttatcaggtt ttgagccttc
    11341 gatacaagac ctcagcctgt tccagaaaag aatcaatgtc ttgtggggtt gtcaatggtc
    11401 cggtagagac acgaattgat gaaaaagcta tgtcatcagg cactagcatg gccttcaaga
    11461 ctctccgttc tgcaagatgg atgttcgaca tacatgctga gccagtagag acagcaatgt
    11521 ggcaatccga caatgctcgc accatagttt ctccacctaa accagggaaa gccattgata
    11581 ttatcgaagg gacaaagccc ttgtcctggg gaattcgttc gggaagtgtg agtgtattgg
    11641 gaatgctgga tatgccatct aagagtcgct tctcaagtgc aatagctttc tcttggtttt
    11701 cctgcatcga tagtgccgct ttttcagcgg caagagaaaa ggcaagtgcg ccaaacagat
    11761 tctcggtacc tggcctcatg ccagactctt gtcctccgcc gcggataaaa gcttccagag
    11821 gtcgagcaat ccaaagtccg ccgactcctt ttggtcctcg aattttatgt gctgatattg
    11881 caaagctatc cgcatagtcc tcaagatctc taattgataa ttttccaagc gcttgaacag
    11941 catctacatg gagccagggc ttttttatct tcttttggag aacagtctcc ttgatactct
    12001 ccgctattgc cttgatattt tgaatagctc ctgtttcatt gttgacattc atgatgcaga
    12061 taagggctgt ctctttgcga attctagatt cgatttgttc ggcttcaata tgtcctttcg
    12121 aatcaggatc gatccttgaa acctgaagtc ctagtctctc caaaatgcgc atttgctctt
    12181 caattgcaga gtgttcgatc gaagatatga tagcgtgcat gggtttttct gcagaactag
    12241 ctcgtttcag cttttcaagg attgcaaaga taggaatgtg atctgcctca gttccaccgc
    12301 ttgtaaaaat aaggctccct tttgtaacac ccatggagcg catgactctc aaacgcgatt
    12361 cttcgagagc ttgccgagcc tcttttccaa gccaatactt gcttgaaggg tttccatatg
    12421 ctttaagact gaattctagt gcaatgcgca ttatgtcagg atcggggggt gctgttgcag
    12481 cccaatcaag atagatgtct ttcatccaga acttatgctc ctaatgccat tttccattct
    12541 gtctcaatga atccgatgag atgttctctg gaatctatta ccataaaagg gaagatgacg
    12601 ttttctttaa atctctgggt aagctcattc ttaatcgcgt tttttgattc aagtggaacc
    12661 agatcgatat aaagatattt ataggcaaaa tgattcacat cgaggaaggt taatgctctc
    12721 ttacaaaaga tacaggtgga tagcgcatat accatgattt catgatcttt tcgcatgcct
    12781 tcttttttca taatagaaat attttctaga ttcattaagg ttctccagat ttcaagtatt
    12841 atatatccga aatggtattt tttgatatag agacagtagg taatccttgg aaggaaatca
    12901 gaatctgttg ataaaagcct ttgtttcgta tacagttggc ctatgattga gcccgccttc
    12961 attcgcaatt tctgtattat tgcgcatata gatcatggga aaagtactct tgccgataga
    13021 tttatcgaaa aggctcacct tatcgattca aggaaattcc aggatcagat gctcgataat
    13081 atggatatcg aacgagagag aggcataacg attaagagcc aggccatcag tcttccttat
    13141 gtagctaaaa atggaaagac ttatatgctt aatcttgtcg atacccctgg acatgtcgat
    13201 ttctcttatg aagtctctag ggcgatcgcc gcatgtgagg gagctgtcct ggtcatcgat
    13261 gctacacaag gcgtcgaggc gcagacgctt tcgaatatgt acatggcttt agaacatgat
    13321 ttggagataa ttccggtcat aaacaagatt gatatggcta gtgccgatat cgaaggcgta
    13381 agaggtcaga tcacaaaaga tctaggtttg gaaggagaaa gcgctcttgc cgtatccgcc
    13441 aaattgggaa tcggcattga tgagctctgc gaggcgattg tagagaggat tccatcgccc
    13501 aagggtggag cagaaaatcc tcttcaggct ctcatatttg attcgcatta cgatccatat
    13561 agaggggcta tcgtacacct taggctaaaa gaaggttcta taaagaaagg tatgcatatc
    13621 aggctcatgt ctacaggggc ggtccatgaa gtagaggagg tggggcattt cagaatggcg
    13681 ctttctccag caatggagct ctctgcaggc gaggtgggtt actgcattgc aggtataaaa
    13741 accgtaaaag aagtacgcgt tggtgatact ttgacagatc atgaaaatcc ttgtaatact
    13801 gcattgcctg gatttagaga agtaaagcca gttgtattca gctcaattta tcctatggat
    13861 tcttcagatt atgaggaatt gagagatagt ctcgaaaagc tcgtactaaa cgatgcatcg
    13921 cttatatttg aaaaagacca ttcacttgcc ctgggatttg gctttcgatg tggatttcta
    13981 ggactgttac atcttgagat tgttcaagag agacttgagc gcgaatatga tcaatcaatc
    14041 attatgactg cgccgtcagt tcgatataag gtgattctca aagatggctc aaccctatat
    14101 atcgacaatc caagcgacta tcctgatccc tcgcgcattg agaacgtaga ggagccctat
    14161 ataaaagcgg agataattac acccgtagac tatataggca atataatttc tctctgcgta
    14221 agcaaacgtg gaagtcagca atcttttctg catttggatg aaaggcgcgt agaactcata
    14281 tttgaaatgc ctcttgcgga ggtcctcttt gatttctacg ataaactcaa gtctatatcg
    14341 cgcggatatg ccagtttcga ttatgtagtg acaggttata ggcctacaga ccttgtaaaa
    14401 ttggacttct taataaatgg agagcccgtt gatgcacttt cgatgcttgt ttataggcca
    14461 aatgcatacg atcgggctag ggttgtctgc gagcgcttgc gcaacaatat tccacgccaa
    14521 caattcaaga ttcctattca gggatccatc ggaagtcaga taatagctag agagacagtc
    14581 aatcctgttc gaaaagatgt tcttgctaaa tgctatggag gggatataac acgcaaaaga
    14641 aaactgctag agaagcaaaa agagggcaag aagaaactaa agatcatcgg aaatgtcgag
    14701 ctggggaaag aagtattttt gtctgtgttg cgctctagtg aggaagagga tagatgaaag
    14761 ggggctctca gtaaaaatgg acagccccct ttattattcc ttgtgctgaa atacctttag
    14821 ttaattagac acaaagagct tcgcaaaagt agcattgaag ttatctttaa atttatccga
    14881 tgttagaatg gaatttcgaa tatggttctc catggcggtc tggaaaaaat agggataaga
    14941 gaattttgta taagaggttt cctgatcgct tgcctctcgg tcttccgtga ccttcatgac
    15001 cactaccgaa gcatctaaga gcacgggttc agatactgca tctttgggtg ttgagaaaag
    15061 cgtggtaagg aaagtctcgt tctctacagc accaacaaga tcggtatcca gcgagcgata
    15121 aggctcttga aaaaggctaa cttgctgacc atagaaatag aaacctggat tgccgtaatt
    15181 gagtataaaa ggacctgctg gctttacctg gagtccgact tttttcgctg cagattcgaa
    15241 ggattcgttc atgagttcag ctttgaattc atttgccttt gccagagccc aagattcaag
    15301 cacactcttt tctctatcaa aaatatagct tttgacttcg tctaggacgg attgatttga
    15361 gaaatcagga gccttcgctt cgctgtttat tcggtaaaaa gcccaggtgt tgccggtttg
    15421 tttgtagacc ggcgaaattt cacccttctt gaggctcatg agattctctg cgtctttttc
    15481 atcaaggaat tcgcctttca aatcgtagaa agtctttgta cccatatcgc cacccttatc
    15541 cgcatagaca tcctttgagt ggctttttgc ggcgtcttca aaagaaaggc tgttttcctt
    15601 gatttgggat aggactttct ttgcgtcttt ttccgatgtg ctaatggtaa tcctggacag
    15661 accaatcgtc ctgaaaagag actcgtgaga cttagcccat tcgagcgttt cctccactgg
    15721 gaagtcggca taggagatgt tgacatactg aattgcgcgt tgtggctttg ccatcgatgc
    15781 tacaaattcg atttccgcag tggaagggga agcagacata aggtcgttgt agtagttttg
    15841 gattagcata tcttcccgga tactatttct aatagaaagg cgggtcgcag caggagtgct
    15901 gttgtatttt tccaatgaga acttgccgtc tacctggaat tgggaattct tagaaatttc
    15961 ttcatcaatt gtctcttcgc ttaccttcat gccagatcgc tttgccgaat ctaggatagc
    16021 cgtgcggatc actgtgtttt gaaacgcgag acgccacact tgataggcgt agagctgcga
    16081 ttgcgtttca tcaagtccct gctgtcgaag gtagtcgttt atctgcgaca cctgctgggc
    16141 aaaataagat cctgaggtat attgtattgg attgccattc cacgatccaa atgaaggaag
    16201 cttagcgcca gcacccgtac ctgagctaat gcttggaata agcacgaatg cgataatagt
    16261 aagcacaaga atgattatcg tgccaatata cacaaagggg ttctttatgg ttttctttag
    16321 agtggtcttg gtttgtttat tatcgccctt tttatcgccg gatttcttaa tgcctgtttt
    16381 tgcttgtttt tcattctctt cggatgccat tcgttgtcct ttcatccata atcctttaaa
    16441 aaagctgcaa taaattatca tgaggctatg gaatagtcaa cgagagtcct ggccatttct
    16501 gcttggagag ataagcaatg gcctctttct cagatagaaa tctcttccat ggattcggat
    16561 catcgatatt ttcatcggca atagctattt caaagggaga tattcgcgcc ttataatcca
    16621 ttttggaggc gccgggaacc caaaagccta ggtaataata gcgcttatct tcggaatcat
    16681 tgtcagctgc ttggcttttt gcatcatcac gtccaagttt gtgctgtatt ctattctcat
    16741 agaaatgaga aaccgattct ccctttattt ccttattcaa caaaaggtgt tttgcctttt
    16801 ttgcgaaact gctcaggtag gattcagcaa ggattgagaa aaagcctaag ctccgtcctc
    16861 ttgcatcagg ttcaaaggag aaataaacag aagacaatcc atcatccaga ggatctacat
    16921 ggcttactgc aagcagtttc ctgcctttac tattatctcg atactcaaat accagactcc
    16981 atggttccaa gaggttttta taagagtctt ctttgaggtc tttgagaggt atggagtgtt
    17041 tccaaataga atagcgctgc catagtctga aatgctcctc atcgaattcc ggtggatttg
    17101 aaataatgat gatatcatta ttttttttta gaatcctatt caaagaaagc gtaagctgaa
    17161 ttgactcagc cttgattctt aggggaatac agagcttgca gccagggcac tccattcgat
    17221 agatttcttt gccatgtctt ctccatccat tcgatatcaa agcactatat aatcgagagt
    17281 caacttcatc ctgaaaaatc agcaagttca tcatagtgga atgattattg gataggtaag
    17341 ggcatgttcc tttgacttga aaataaagga cgtatctatt ctccgaagtc atgtctattt
    17401 ccttaaatcg cgctacatta atcttaaggc attaaatctg cataaaaaag ctctctagta
    17461 gcttgattgt tggtcttagg ctgtataatt taactatatg agcattatta ccaagacagg
    17521 cgatgagggc acaacaggtc tctggtctgg tgagagaata ggcaaggatt cgccaagagt
    17581 agaagcatat ggtacaatcg acgaactctc atcagccctc gggatggcac gtcatctctg
    17641 catgcaggac aatgttcttt gcgcaatcga gtatattcag cgccttctat tcagagtagc
    17701 cggggaattg gcatctatga gcatgccttt tgataatcct atcaacgaag atgacgaaaa
    17761 gacaattgaa gacaagacca atgaacttga aatgcgcatt cccataagga gcttcgtcat
    17821 gccaggaatg accgggggtt cggcggcact agatctggcg aggacaattg cacgcagagc
    17881 ggagcgtcgc gtagtcgctc tttctagaat agagcatgtt tcgcctgttt tgctgcgcat
    17941 actcaatagg ttgtctgatt atatatacat gctcgctcgt gaggaagaat cgaaacaagg
    18001 aaagttgacc tttatctaag atttcttgga cagagcgctt tgcttgctga ttccataact
    18061 caagcggaga agcgagaatt aaggaggtat tccaaatgag gctctattcc aaggcgcaga
    18121 taggtttggt tattgttgtc tccgtgactt tgacagctct ttcaattgct ggcatagtat
    18181 ttgttcttga tagatctgat caaaaccaat caactatggc tgtcgcttct aaaatagctc
    18241 aagacggcga tgaagcggtg tacatcccag aaataactag cctaggtatt caggatgata
    18301 agaccgcaag tctttctctg caagtggcga attcaaccct gagcatgagc agttatagtg
    18361 cggatgagat cgagaatatc acaatctatg aaaagtacaa cgaatctgtg gtcaacatca
    18421 acaccgaagt gcttggtata aactggtttc tggaacctgt gcctcagagt ggcggatctg
    18481 gttccgggag catcatcgat gagcagggtt acattctcac caataatcat gtggtagaag
    18541 gagcatataa gctctatgtg agccttgcag atggcagtcg ttatgaagca aagctaattg
    18601 gtacagaccc cgagagcgac ttggccgtga taaagatttc tccggaacca ggaaaaaagc
    18661 tgaagcctat tccttttgga acatcgaaag atcttaaggt aggtcagaag gtattagcaa
    18721 taggcaaccc attcggtctc gaaaggacat tgacagctgg aattatctct ggcctagggc
    18781 gtcctatcca ggaaagcgag acaactattc ttcagaatat gatccaaacc gatgcatcta
    18841 tcaaccctgg caactccgga ggtccactat tcaatatgaa aggagagatg atcggaataa
    18901 ataccatgat ctattcgcca tccggaggat cggtaggtat aggttttgca attccagtag
    18961 atacagcagt tcgcattgct cctcagctca taaagaacgg ccaagtcaag agaggttgga
    19021 tcgagatgca ggtaatccaa cttttcccgg atcttgtcaa ttatctacgc cagagtggaa
    19081 aagccgcacc tgtagagact gggcttctcg tgtcgacagt gacttctggt ggcaatgcag
    19141 aaaaagctgg cctcaaagga ggcacaaccg cagtgcgcta tagatcgaca gtattctaca
    19201 taggcggaga tattatcgtg agtatagacg gcaaacctgt caccaaaatt gcagaactat
    19261 atgcagcgct ggaagataca agtcctgggc agcgcgttaa tgtcgaattc tatcgtggtt
    19321 cgaaaaggat gagcactgta gttattcttg cggagcgcag gaggtgaagc cctttaaagt
    19381 tgtgtccccc tttgagccag caggcgatca agctcaagca atcgagacta tttcgagcgc
    19441 cattaagagg ggtgagaagt atttgacact caagggggta acaggttctg gaaagacata
    19501 tactatggca aaagtcatag aagctattca gaaacctgcc cttgtgattt cacacaacaa
    19561 aaccttgtct gcccaactat accgcgagtt caaggaattt tttccagaga atgccgttga
    19621 gtattttgtc tcctattatg actattatca gccagaagcc tatgtacctt ctaaggacct
    19681 ctatatcgaa aaagactctg atatcaatga agagattgat cgaatgagac tttctgcaac
    19741 cgcctcgctt atggagcgca gagatgtagt gattgtcgcg accgtctctt gcatatatgg
    19801 attgggttcg ccagatcttt atcgggagat gagattgtat ctagacaaag gccaaatagt
    19861 cgatatagat gaaatcaagc ggaatctcat tagcctccaa tatgaacgaa acgatatggt
    19921 attagagcgc ggtcgattca ggattcgtgg ggatgtcctg gagatttttc ccgcctatct
    19981 ggaggaggct tatcgaatcg aactggattt tgatactatt gtgcggatac gtcgcctaga
    20041 tccgcttact ggtgaggcct cgaatgagtt agaggaagcc ataatctatc cggcaaagca
    20101 tttcgttatg ccagaagatt tggtacagaa ggcggtagat agcataagaa atgaattaga
    20161 ggagcgatac caattcttca atgctcatgg caagtatctc gaggcagaac gcataaaatc
    20221 gcgaacagaa tacgatatag agatgcttag cgaaatggga tattgcagtg gtatagagaa
    20281 ttattctgcg ccgctgtcaa accgcaagcc aggagaaaga cctggagtat tgctcgatta
    20341 ctttccgaag gattttattt gttttatcga tgagtcacat gtgacgctgc ctcagcttgg
    20401 agctatgtat aatggcgatc atagcagaaa attatcgctt gtcgattatg gctttcgatt
    20461 accctgtgca cttgataata gacccctaag aatcgatgaa tttgagtcga tagtagcgca
    20521 gacaatctat gtctccgcca caccaggtga gaccgaactt cgacgatcca agactctggc
    20581 agagcaggtg attcgtccta cagggcttgt ggatccggag attcttgttc atcctactga
    20641 aggccagatg gaagacatct atgcgcgagt gcgtgaaaga gccaggcaag gcgaaagaag
    20701 tctcataata acactgacaa agaaaatggc cgaagagttg acagattatc tgagtgaact
    20761 tggtcttcgt gttcgctata tacactcaga ggtagagact atcgagcgtg ttgagattct
    20821 aacacagttg cgtgcgggag aattcgatgt actggtagga ataaaccttt taagagaagg
    20881 catagatctt ccagaagtat catttattgc aattctcgac gccgataaaa ttggtttctt
    20941 gcgttcagag acaagtctta ttcagatcat aggaagagca gcgcgcaacg cggcaggcct
    21001 tgtagtaatg tatgccgatg tcatgagccc agctatgaaa aatgccatta tggagaccga
    21061 acgcagaaga aaaatgcagc aagcttataa ccttgaacat ggaataactc ctaggacaat
    21121 tagcaaaaag gtccaggata tactcgagcg ccataagcag gagagaatag aggcttctca
    21181 tggcgaaatc gagatgctta ggcgcaccca taacttgctg gttccagagc aaagaagatc
    21241 cttaataaaa gagcttgaga agcttatgtt cgaacatgca aagaatttgg aattcgaaca
    21301 agccgcttta atacgcgatg agatttcaaa attaaaagaa taacaaaagg attagttatg
    21361 tcgaataacc ataatcaaga ggatttctat gtgatcgatg cacactgcga tacgctcatg
    21421 gctatcgtag gtcgcagtat gaataaggaa gagagagatc cacggaattt ttttgcagat
    21481 aataattcgg tgcatttgga cttgccaaag ctttccagag gtcatgtggc atgccaggta
    21541 atggctatct ttcttgagga ttcgcagctt tctgaggcta caagggaagc catggccatg
    21601 atagattcgc tcgagtctct cttaagattc gaccccagag aattctttct tgcgaggaat
    21661 agcgcggaca tatatagggc tctggagaca aaaagtgtat gtgctttgct ctcgatagag
    21721 ggggcagaag cattgggcga ttcattggag agcttagaaa tattctatca gcgcggagtt
    21781 agggctattg gtttgacctg gaataggcgc aatgcttttg gtcgaggcct aaaagccgaa
    21841 ggaagcgatg gtcttaccaa tctcggcaag gagcttgtcg agaagatgga ggaaatccat
    21901 gttatcatcg atgtggcaca tctaggcgaa gaaggcttct gggatgttgt tgatatatcc
    21961 actggtccat ttattgcttc tcatgcgaat gctcgggata ttctcgatat tccgaggaat
    22021 ttgagcgatt cccaaattag agcaattgct gatcatggtg gggctattgg ctgtgtgttt
    22081 gttcccggtt ttgtcacaaa agatcccgga gactgtagcc tagaggcatt cttgcttcat
    22141 atcgatcata ttgtgcgcgt tgggggtatc gaaagctgtg ctataggttc tgatttcgac
    22201 ggatttatgc cgcaaggcgg caaggtgatt caagattcag gggagtttcc gcttctttat
    22261 gaggcgctga gaagccatgg ttatgatcat gcagcagctc agaaaatact cggcgggaat
    22321 tggctaagaa tctttgatga cgtgcttggc ttagtatgaa gaagatcgcg ttgattggca
    22381 tgatgggctc cgggaagagc gaaattggtt ctctccttgc tagtaattat ggaattgact
    22441 tcattgatat ggatagagct atcgaagagc acacaaagac cacaatacca acaatcttcc
    22501 gcgattttgg agaagagcat tttagggatt tggagcaaaa ccagctcgcc ttgatggctg
    22561 tgagtttcag ttccctagtg ctttcctgtg gcgggggcat agtcttgcgc gactctaacc
    22621 gcttgatcct aagagattcc tttcttacta tctggttgga tgtatcgata accgaactga
    22681 agcgaagact ccaaaaccaa agacatcgaa ggccgcttct tctttcggat gagtgggaat
    22741 cacaaataga agagatctat cgtcagcgct atgagctcta taggagtaca gcaagaatac
    22801 aatacacctg gaaggataat cattcgatgg aagagagtgc aatcgatatc aaaaggctca
    22861 tcgatgctgc ttataatggt atttcaggaa gaacttgaca cataccctga tctattgaaa
    22921 tctcaatttc ctttccttga gaagacattg aaaagtcgga tataggcgta ggtaactcga
    22981 aaaagtagtc ctgcatttcg ggaatcgtca agaccagttt ggatgccaga ggagttgcac
    23041 tgatgagccg gactattttg actttcattg tctttctgtt gttgtttttt ccaaagatct
    23101 ctatagcgct aaacgggata tataggcaca ttgctttttc caatgcctta tcgatattgc
    23161 ttaccgaatt gcgacttact tgaaacgtgt ttccttcgga agtcattata ttgaggagtt
    23221 ggctctcttt tgaggccgag atttctgtta ttggaagcac aaggccaagg ttcataaatc
    23281 tggcaagcca gccagagccc ggttttttct caaagtcatt gataattcct ctgctgatca
    23341 ttttgccatc gcgcataacg atgatttcat cgcccaaatc gatagcctct tccacatcgt
    23401 gtgtaacatg gatagttgtc aagcactgtt ttgagagctg ctcccgaata aaacgtcgca
    23461 tctctcttct caggggtgca tctagcgaag acagtggctc atccagcaac atgagcaaag
    23521 gatcggatgc aaaagccctg gcaaatgcaa cgcgctgtcg ctcgcctccc gataactcaa
    23581 aggggtaacg gcctaaaagg tgctctattt tgaagttgcg agccaaagaa attacccttt
    23641 cttctctctt tcttttttct tcgcttcgaa taaacaggct atatccaata ttatcccgga
    23701 ctctaagttg ttcgaatagt gctaaatcct ggaagacaaa tcctatatgt cgtttttctg
    23761 tgggcaatct gtcaattcta atgccattca acgtaatagt gccctccttt atcatctcga
    23821 gccctgcaat cgagcgaagg agagttgttt ttccgcatcc ggaaggacct aacaacgcaa
    23881 gaatctttcc ttcctccaaa gaaaagtcag caaaaagcgc aaaactatcg cgttcgataa
    23941 gcaaattatt tacttcaagc gacatcgact gctttctcct ttagcatgaa tacgataccc
    24001 gtcatgagcc caagtacaag accggcggca caagcttcat tgaatcgata cgccgcagtc
    24061 aggcgataga gaagaagagg gagtgtttca atctcgctca ttcctagcat aagtggtaca
    24121 ttgacatctc ctgcggttat ggagaaagcg aaggctgcag ccgccgccac cgaaggtaag
    24181 atcgtccgca gttcaattgt cataaaagct ctcagaggtg aagctccaag tgttttggcg
    24241 gcttcatgaa ttcgaaaatc tatggcacgc agcgacgcgc ccaccgttct actcacaaat
    24301 ggccatgcaa taagagcttg agcagcaatg agagcataag tcgctatatg gtttgcagat
    24361 ataaaaagcc atccatatgc aagaactgcg ggcgatacag ccattggaag ccattgcagc
    24421 agatccatca taagatcaat gagcatgctt ttcttcccta ttctctgagc aaggaatgtg
    24481 ctaaagctcg caagaatccc aatagaagtt gccaacaacg cagcaattcc actcaatttc
    24541 aatgtagaaa gaattgctcc ggttaatggc gcttgaaatc ctgtaaagag tcgcttgaag
    24601 ttatcaacgc caaatctctc agggctattc aatgacgctg gtactgtaaa tgcttcgatc
    24661 acgatcgaaa aaagcggtcc gataaagaaa actatgatta agatctcata aagaacgatc
    24721 aaaaaccatt gttttccctt gggttttgcc aatttgtgcg ctgctccaaa gtctctcacc
    24781 gtcctacgcg tcttactctg aattctcgtc aacatccata ttcctaaaag cgcgcaagcg
    24841 ctctctataa tagaaagaac tgctgcaagg gccagatttg aacgatatcg caaagcctgg
    24901 tatatttcga cttcgagtgt cgttcccgcg cgaccaccaa atacgagcac tgtagtaaag
    24961 ctaaaaaagc aatataagaa gattaagcct gccgattggc caattccagg cagtatccag
    25021 ggcaaaatac ctgttcgaaa ggcttttgat ttccttgctc caagagttct ggcagcttca
    25081 aaagaagtat tgggaatgcg caacaataat gctgaaatct ggtgcacaac aataggaaag
    25141 ttatagaagg catgtacgat aaccagtccc caaaaactgt aaagaattcc tctgtattcc
    25201 cctctcgagc caaagactag agaaagagcc gttggaagta gtccctcttt tccgtaatag
    25261 catataaagg cgagaataat taggatcgga ggaaaacaga atggtatagc agccaaggac
    25321 agaagagtct tccttccagg aaatctgaat cgggccgcca gccatgcccc aggtagccca
    25381 aggctaattg ccagagcagt gctggcaaag gattggcgta ttgtaaacca aagacttttt
    25441 gctatcgtcg attctggtat ctgcttcaag aagtctagaa tgatctcaat gcccccaata
    25501 ttgcctttta tctcacttag accaaatatg gcagcatgca agaatgcaat gagaggggcc
    25561 aaagagaaga tcagaataat gcaaagcacc agatatagaa aaatcttggc tctcatggat
    25621 ttccaatgag aagatcggct gcttctgaag gatcatggtc gagatctcta attgttgcac
    25681 tcaaagccgg atatttttgc acgatagaga acgaaggagg cagttcagtt ttgatgttag
    25741 cgggatacat ccattgtgtt tcaggcagca aagcttggca ttttgcggat aatagatagt
    25801 ctataaattc ttgtgcaagt tctctcttct tactggaaat cagtacaccc gcaaattcga
    25861 tctgtgttgg atgcccttcc acaaattgaa gggcttgata gcgctctgag ttttcaaaag
    25921 ccttatggta tgcggggcta gtggaatagc tcaaaactaa aggggcttcg ccttttgtaa
    25981 agaggccata tccagtatcc cagctcggtg tca
//
LOCUS       DAOMZL010000019        25901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_1577_length_25901_cov_16.548596, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000019 DAOMZL010000000
VERSION     DAOMZL010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 25901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25901
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1577_length_25901_cov_16.548596"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2031
                     /locus_tag="PLQ30_02875"
     CDS             <1..2031
                     /locus_tag="PLQ30_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPW01417.1"
                     /translation="RLIFSPNGDGRADTVELIQSSVYGDRWNGQIISETGKIVRTWEW
                     YPMLADVLWDGKDQSGKLVSDGVYYYELRSIDAAGNFFILPRQQIIVDAAQKNVSFKV
                     EPTAFSPNDDGVKDLIYLNVNAPKPDTLLSYSLSIFAGSKAAAGQAPVRSWKGKIDIK
                     SQYVWDGMTDAGLKVPDGYYSTYLELEYANGDLFNFGPIQILVDTVPPRISISADPLL
                     FSPNGDGIKDTITIAQESVPGDDWSGRIRSASGATVRSYTWKGQAKNFTWDGKDSNGK
                     LVPNGAYSYEVVSVDAAGNSASASIKGIMVDATKPRVFVTASDNGISPNSDGIRDEVS
                     FSLTVENREGVDSWRFSLLDSKGVERSFFGGSGSDVPTRLVWDGRDLNGQVIEDTFTG
                     KLIVRYMKGDVAEATSSKIVVKIAPPQVEIKVKPEYFSPDGDGVDDMLTFGIVADKNA
                     GITEWKLEVLETAIVESAALQESKPTRSFMLWSGKGMPPSQITWNGKSPKGELVESAT
                     DYPFEFTCWDALGNQTKISGIIAVDVLVIRDGDRLKIRVPSIVFRANHADFIGLDSEI
                     VARNEKVVTRIAQILGKFPDYRIRVEGHGNNVGKMLGYSASRIQQEEINELIPLSTER
                     AEVVRKMLVENGVDARRLTVVGLGSSEPVVPFTDVQNRWKNRRVEFVLIKNQ"
     gene            complement(2183..3061)
                     /gene="murB"
                     /locus_tag="PLQ30_02880"
     CDS             complement(2183..3061)
                     /gene="murB"
                     /locus_tag="PLQ30_02880"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPW01418.1"
                     /translation="MKEHTTFKIGGPADLFIRPRSISALEEVVELLKIENVPFFILGS
                     GANILVGDRGVRGAVIDTSLLSSIMHLKREERIDPVLLYAECGVRLMTLCEEAQICGL
                     SGLENFYGMPGSLGGSVYMNARCYEDDIATHIQDLTIIDNQGRLKIISACAQSWSYKR
                     SPFMRGGELESLIICAASFRLIPDDPEKIAERMRLCMRDRMQKRHFNFPSAGSMFKNN
                     HDFGKPTGKILDELGLRNYRIGDAAISPWHANIFVNIGKASARDMRKLIEYAQKVVYD
                     ATGFCIEPEVLFIGEF"
     gene            3197..4624
                     /gene="cysS"
                     /locus_tag="PLQ30_02885"
     CDS             3197..4624
                     /gene="cysS"
                     /locus_tag="PLQ30_02885"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPW01419.1"
                     /translation="MHDVHIFNTMGRKIQLFTPLHEGNVGFYGCGPTVYNYAHIGNLR
                     TYIFEDTLVRMLRRFGYRVTHVMNITDVGHLSGDDDTGEDKMLKSAREKGQSVLEIAQ
                     FYTNAFFKDTERLNIRQPDIVCKATDHIQDMIELIKRIEANGYSYFSGGNLYFDVSKF
                     DHYGELAGLQLDQLKAGARVELDPNKRNPFDFVLWFTKSKFDKQALIWDSPWGIGYPG
                     WHIECSAMSMKYLGEQFDIHAGGVDHIAVHHTNEIAQSEAATGHRWVNYWMHAEFLVL
                     DKGKMSKSSGSFLTLESLIDKGYDPLDYRYFCLGGHYRSQLSFSWEGLDQARSARLSL
                     LDRVLALKEKAVKPASISELGQKALRYLTAFDDALADDLAMPRALAQVWSLLKDNDVP
                     AQDALALILNIDEVLGLDIAKAQRREVSVDTDTVQWINSQIQRRAAAKKARDFATADA
                     IRSELVARGIQLEDRPEGTVWRLIE"
     gene            4661..5257
                     /locus_tag="PLQ30_02890"
     CDS             4661..5257
                     /locus_tag="PLQ30_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPW01420.1"
                     /translation="MFQNKDDGPISDYNLIFAQNVDLLLRIAVHITNDWESAEDIVQD
                     AFGKLIEKQMQFPSEEDARFWLIRVVKNGAINWSRRKVREFKAYEHWWNAETTQAKIS
                     ADALDSRDMQNQHAGTDQSADHELMMKECAQEVRDALMRIPEKLRMVLILKEYEGMNY
                     KEIAKVLGITEGNVKVRAFRAREALLSILNEGGSYVSR"
     gene            5244..5864
                     /locus_tag="PLQ30_02895"
     CDS             5244..5864
                     /locus_tag="PLQ30_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01421.1"
                     /translation="MCPDESLLTAYIDNEVPSPWKERIEMHLCQCERCANRVAQYRAL
                     KVALKAADVLEASKRQEAAKRIQASLKSITDESSRPLRTEGIIERYPVFSTFMSKRIS
                     VPLPILAASLLIMVFFAGLAFGIFGAHRTTGQALALSTRLPEESASNIESIVSLLSQT
                     DPNQFVTIHAPGNISQPLVNTTPVYVIYNSSGRKPTVMAFPVEGER"
     gene            5866..6429
                     /locus_tag="PLQ30_02900"
     CDS             5866..6429
                     /locus_tag="PLQ30_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01422.1"
                     /translation="MKRSTLTLAAISILNVAWLFAQTPSIPTAPSSIAIPDSPSSNIP
                     VFQFQIRTAFTLTPGMVSAIQSASLGSQGSKAAQGESIEKANSPKWEDAIVREVSLAS
                     PLGIKIQNDRFIAIIVLMPIELIKKDITMLVQNHIYMRSQDNSIQLNTSVHTVRIPVD
                     SVFYYYPLGGDSKQGAPMVIEILVNSK"
     gene            6434..8068
                     /locus_tag="PLQ30_02905"
     CDS             6434..8068
                     /locus_tag="PLQ30_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPW01423.1"
                     /translation="MKQRRIITLCILIFLVAAGIIIYRNMQPRLLAIDPVIVMSGDLV
                     NIAGRNLGKKEGELLLDNVPLPSQAILFWSPFLINFRMPSNYDSAVVRVKTGLGVSNP
                     LMVALASKVPSKAENNLIQQHNPSITSIKPSTNAQIGKLIELRGSHFGMPEEESAVLF
                     SKVPIISTLEAIDADNFARVESISLLVDKWNDSYIAAYVPDGTENGYVLVRTRNGLSN
                     AFPISISRNPGRLSRGDSVTYTLKDTLVVHIAASLPDGRVSFILSKPPVTQYQSTMAT
                     IEAGADHLRAETAVWQEFRLGAADARLGRIELQRRLIIDVCEITADITIGTLQPLGTK
                     PPAFLSPYLAEDALVPSTNPSIISAALSMKKKEKNPFRQIMLATQWTFSNIKLKQEKQ
                     SFGDDPIDALKSKAGGIRSLVLINCALLRALNIPAIPVAGFLVTEDMRLIPHYWSEYY
                     LIGIGWIPFDPALAIGMIPSGFRPVFSNRSNYYQGIDNKHIAMARGYQLLPSIPAEAQ
                     KKSKIPWSLFEYDEVAHGLSYTSTWNPPILLSTTIQ"
     gene            8172..9437
                     /gene="glyA"
                     /locus_tag="PLQ30_02910"
     CDS             8172..9437
                     /gene="glyA"
                     /locus_tag="PLQ30_02910"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPW01424.1"
                     /translation="MDPELYAAMEAEASRQREKIELIASENYTSKAVMEAVGSVLTNK
                     YAEGYPGKRYYGGCEFVDIAENLARDRARELFGADHANVQPHSGSQANMAVLFAALNP
                     GDTIMGMSLAHGGHLSHGAPVSFSGKFYKVVNYGVNPDTELIDFDQVAFLARQHKPKL
                     IIAGASAYSRIIDFDAFRRIADEVGAYLMVDMAHIAGIVAAGRHPSPIANAHFITTTT
                     HKTLRGPRGGMILVGRDTENDRGIMNAKGDALRRWSELIDSMVMPGIQGGPLMHVIAG
                     KAVAFKEAQRPEFVEYIDHVLSNAKVMAQALTLGGARIVSGGTDNHLMLVDLTPLGIN
                     GRDAERWLDEASITVNKNSIPFDKKSAFVTSGIRIGTPAITTRGMGPSEMEQIASFVM
                     EVLRSKGDPQVIARIKQEVIGLTSRFPLP"
     gene            9445..10686
                     /locus_tag="PLQ30_02915"
     CDS             9445..10686
                     /locus_tag="PLQ30_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HPW01425.1"
                     /translation="MQKIEHSELYEQAGRQAAYRLLSNAEEQGFFSYGILTRQAWEQI
                     VEEQGTVQADCREGKPLSLNSLRTSSPWTEQLKAESSPVMALKERYHLSDVGAMLVVA
                     LRYAPDVEAEEAAARALRGLPPLPLARDGRPAARVARFARGNWYREILDRLRNCATAT
                     FGAIIKEGSAERLGLEAKPPQRWPRFVNSIFPEKALALAAGLGTIGRNGLLVAKKTNN
                     SDIFDALDFQEHLIASDSSKPTTTNPPTWSSAVLIGLMLLPFDFDMLKLEKKDIFGGV
                     APSFFAVCGNCRLCIESCPSRALSFSSLPGFARHLCIQNYSSIDGNLPDFIEKSWADQ
                     LYGCDLCLEACTYFEPDQKAHTNRGRIGGFFDAESLADLSEEQIQALFKASALDQKWI
                     APGALKRNAAIVIQKFHKLRN"
     gene            10865..11236
                     /locus_tag="PLQ30_02920"
     CDS             10865..11236
                     /locus_tag="PLQ30_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01426.1"
                     /translation="MKKIIAVFAIISIAFFACAPKKTNIDNQDSKSVSNMAPEEIKDV
                     ATWKAAYDDLISAYSSAVAQLNGGNLGAGAKIEELGKRAEALNSVAETIRSGLSGQEQ
                     ADFAQVIEAYREKFSQAVNSN"
     gene            11372..12334
                     /locus_tag="PLQ30_02925"
     CDS             11372..12334
                     /locus_tag="PLQ30_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01427.1"
                     /translation="MNIDLINLRSRMISIVREFFISKDYLELDTPALAPYLIPESCLE
                     VFATEYIHPIYGHRPLYLIPSPEIWMKKVIANTNRSVFQICKAWRNAESVSPIHNPEF
                     TMLEYYGVGLSSKDNIEITEELFEALAGTETPSRVRPPFRVMSMKEAWAEFAHADLDA
                     LGDDEAMREACRRRGLLVPQTAAWEDAFHILFLSIVEPSLPKDKPLILDLYPSRIECL
                     AADIPGTRYKERWELYVEGIEIANCFTEMADPEAVDRYMQSQSKKKRESLVPHIIDEA
                     YSSIFKDFPPCSGVALGFERLMMVLCGKKSIEDVMIFPFSSFFG"
     gene            12495..13058
                     /gene="efp"
                     /locus_tag="PLQ30_02930"
     CDS             12495..13058
                     /gene="efp"
                     /locus_tag="PLQ30_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPW01428.1"
                     /translation="MIRGGDIGIGTCLIINGVPHIVVEREFVNPGKGAAFARVRAKNL
                     KNGYVVTQTIKTADSVEDAQVDLVDCQYQYSDDESFHFMNNETFEQFEVPITAHEDRA
                     PYLKEGETYTIVMWEGEPIDIKIPYKMVFTVAESENYVRGDTVSGATKPVRTETGLVV
                     RVPLFIKQGEKILINTETNEYVERVNE"
     gene            13039..14460
                     /locus_tag="PLQ30_02935"
     CDS             13039..14460
                     /locus_tag="PLQ30_02935"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPW01429.1"
                     /translation="MSASTNKADDSFDSFCQLDPLVNKRVDFAYRGTKLKFELSYSLF
                     SSFDIDEGTRFLLKEISNEQGIIHAHRLLDAGCGVGVIGISLAASCPEMDVVLRDRDF
                     RAVALSARNAERNGLGVKLFGLNGQQHVLCQKKKFAKIKIKQRKADDLIAEPGLLCEP
                     DERGPYDAIVSNLPAKAGVNVLENYFHFACTELLQPGGTFAFVIIAPLADMACIWCAN
                     AGLALIRTNRTKNHMVAIAQSPREVQVGKLSSNGWISAYLRSHTERQLARANLEWDGF
                     QGLPEFDEPSFVTLCTLSLAQRVFAGLLVRRALVVEPGVGIIPIWIKEVLGTEEIVLI
                     SHDVLALTASIHNLEKKGFLKAISILDPILPIISPSAIENSLPHEALLQEKEGQVSVE
                     KKLSPASIDVAVFFLEDIPRYNIASASWLLTQKSLKRGGVVIFSGTSTHCERVCREKP
                     MGFSRLPHSDQKKGWHAVAFRRD"
     gene            14582..15100
                     /locus_tag="PLQ30_02940"
     CDS             14582..15100
                     /locus_tag="PLQ30_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01430.1"
                     /translation="MKKAERMILESRTPDEYVENSLKSRLSPAEKAKLARLWMQGTGF
                     NREDIIHARNRSEYWRKRKMAGTRERTKRRMAQHDYSKGKTIVWTRENIAEFLSLNRK
                     DAYGRYLYRDWELAALFSTSIPSIQYLRRKFNKVSKMLGPGARRDKVIDYMTCSELVL
                     QRGGPKALKSKR"
     gene            complement(15107..17155)
                     /gene="recG"
                     /locus_tag="PLQ30_02945"
     CDS             complement(15107..17155)
                     /gene="recG"
                     /locus_tag="PLQ30_02945"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPW01431.1"
                     /translation="MFLRELTQDISILRGTGPATLRSLANLNIHTIADLLLHLPRDYE
                     DRTKILPLSRFSEGKVYSKAKVIRHEWFGFGRMRTLKIIIADESSEAALLCFNRPFLE
                     QMAPVGSTIQVYGKFQFKYGEIQSSAFELKTLQDNFVPERTLSPIYPLTGRLNQTTLR
                     RMIALALQKFALHLEDELPGTLRELYNFPSKSEAIRSIHFPSSWDSLKSAHDRLAYEE
                     LFYFQLGIALRIQSRQTKTINRIAVPGVLARQLRERLPYTLTEDQELVLKEIIQDMHK
                     PFPMARLLQGDVGSGKTMVALLAALYAVERGGQVAIMAPTELLARQHATIAAHLVEPL
                     GVRLAFVTGSISSAVRPQLLEALKSGDIDIAIGTHALFSDDVSFKNLELIIIDEQQRF
                     GVLQRIALYKKGHVPDFLMMTATPIPRSLALTFFGDMEVSTIRHLPPGRKPVTTHLAN
                     ESRRDSVYEFTRKKLKEGQQAYFVYPVISESDRLGLRDAESMAEHLSKKIFPEFKVGI
                     LHSRLNDDAKMDVMSRFSEGEISVLVATTVVEVGVDVPNATVMVVEHAERFGLSALHQ
                     LRGRIGRGDLQGYCFFVYSKDITEEGRMRLKALYETNDSFAIAEEDLKIRGPGNMFGA
                     EQSGDLQLRIADMRSDFDLLKKARNDAFRIIEDDPSLNSPDNRVIRQVLDRANPYSDK
                     "
     gene            17229..18728
                     /locus_tag="PLQ30_02950"
     CDS             17229..18728
                     /locus_tag="PLQ30_02950"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HPW01432.1"
                     /translation="MQKETARLIELDREHTLMSHIGAVLGWDQETYMPSKAIEERASQ
                     IALLEGLAHQKAVNPEIDELLATLEEKHDLSLDERAYVRKMRRDYDRETKLPEAFVTE
                     YAKTTSIAQAKWAEAKKNNDFRAFEPHLEKIVDLSRSLAGYLNPNAKPYDVLLDLYEP
                     GNTQQSVASIFSTMKAYLVDILDKIRGRPQIEDRCVGRYVDSETQERISLYFMNVLHY
                     EMDRGRLDVSAHPFTTSLGADDIRITTRYIEDYFPSSLFSTIHESGHALYDMGIDPDP
                     EFRGTKLAEAVSMAVHESQSRLWENIVGRSRVFWERNFLALSALLGKAGEDLDFKSFI
                     ESINRVSPSLIRTEADEVTYGLHIIARFELESALLDGNLRVTDVPGAWREKYKELLGL
                     EVSNDREGCLQDVHWSIGYFGYFPSYALGNLYAAQFWAKLKEEVPDIEARIASGDVLS
                     VLTWLRANVHCHGSRYMPGELVEKITGSALDPAWFERYLREKYSSIYGF"
     gene            complement(18732..>19789)
                     /gene="hisD"
                     /locus_tag="PLQ30_02955"
     CDS             complement(18732..>19789)
                     /gene="hisD"
                     /locus_tag="PLQ30_02955"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPW01433.1"
                     /translation="DSNDSNRPTTLNIATGEMAAKRNTGRGEKGALVGRHGHDLVPVA
                     RVGCYVPGGAFPLPSSAIMTVVPAKVAGVEEVWCAGPRPSNETLAAAWVAGADGFLQV
                     GGAHAIAALAFGICIPRCDIIVGPGGKYVMAAKRQLFGIVGTEAPAGPSELLIIADKT
                     ADPDMIAADLLAQAEHSPDAMPAAIVSEETIALEIEKSLMTRLEDLPEPNQSIAYRAL
                     SNGFLCVEQDLLRIVEGANRCAPEHLEIATSDPVGLMKQIKNAGSIFLGIGSAEVFGD
                     YGAGPNHTLPTGGASRFAAGLSVLCFLRARTWLAIENPELLAEDTAAFARLEGLEAHA
                     QAALARVHKHKNKGPDS"
     assembly_gap    19790..19889
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19890..20273)
                     /locus_tag="PLQ30_02960"
     CDS             complement(<19890..20273)
                     /locus_tag="PLQ30_02960"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPW01434.1"
                     /translation="MEKHPLKRIAIDDIAEPSVRLFDPEIEAVAKQAIDAIRAGKEPE
                     LRRWAERLDGLPVGAPLVISRDELKAAYDKLPKKTTELMERAKGRVAAFAAAQRACLT
                     PLELPIRIEPIKMKSQMYSPEARRPP"
     gene            complement(20255..21568)
                     /gene="hisE"
                     /locus_tag="PLQ30_02965"
     CDS             complement(20255..21568)
                     /gene="hisE"
                     /locus_tag="PLQ30_02965"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HPW01435.1"
                     /translation="MIVPSIDITNGRAVQLRGGKQPPLDAGNPLELAERYGRVGEFAV
                     VDLDAAKGVGSNRELILSLCRIYRARVGGGIRTIDLAMDYLNGGARSIIIGTSATPEF
                     LSSLPRERVIAALDSKEGNIMVEGWSKQREGSIEESIIALAPYVSGFLITFIETEGDQ
                     CGIDIERARKLIALAPDSKFTFAGGAAGGERGIADIATLDRLGADLQAGTSLVLGDLS
                     LAEAFAAPLITDRADGLWPTVVCDESGKALGLVHSSKESLKLALNCGKGVYHSRSRGL
                     WVKGESSGNDQILLRAELDCDRDTIRFTVRQNGEGFCHLGRRTCFDNGYGLEKLDRTI
                     KRRLKEAPQGSYTRRLFADEALLASKLREEVQELIQSKSLQETISEAADVLYFALVKT
                     ISMGGGLSDIEEELERRSLRVTRRGGNAKPEFEGLEGKSEWKSIH"
     gene            complement(21565..22332)
                     /gene="hisF"
                     /locus_tag="PLQ30_02970"
     CDS             complement(21565..22332)
                     /gene="hisF"
                     /locus_tag="PLQ30_02970"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPW01436.1"
                     /translation="MNGLAVRIIPCLDFKDGRVVKGTRFLNLVDSGDPLERALLYERQ
                     GADELAFLDISATLEGRKTMAQEVARIRQVLSIPIMVGGGIGSIEDACRLLEAGADRI
                     SVNSAAVRNPLIIDELASRFGVQCVVVAIDVAENSTMPSGYEVKIDAGSRKTGFDAVA
                     WAQEASGRGAGEILLTSIDRDGTSLGYECRLIGLVARAVLVPVVASGGASKPEHFLEG
                     YLSGASALLAAGIFHRGEISIREIKEFLKAHDVEVRL"
     gene            complement(22329..23027)
                     /gene="hisH"
                     /locus_tag="PLQ30_02975"
     CDS             complement(22329..23027)
                     /gene="hisH"
                     /locus_tag="PLQ30_02975"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPW01437.1"
                     /translation="MKERKKLIIIKTGAANLASVEAFAKRLGFEPVVSDMMIDVLEGQ
                     VVILPGVGAFGPAMEKLSQDGLDEAICRRVEQGLPTAGICLGMQLFFEASEEAPGSKG
                     LCIFNGTVKRLAGGLPLPQLGWNKIESESSAHLLESGWVYFANSYRVGEVAYTYKTTI
                     KGSKGAPFAQNVFDQVAIASARYGEPFVAALEAWQDAKPTLLLCQFHPELSGSFGTRL
                     FSHWIDLNRIGRNV"
     gene            complement(23030..23632)
                     /gene="hisB"
                     /locus_tag="PLQ30_02980"
     CDS             complement(23030..23632)
                     /gene="hisB"
                     /locus_tag="PLQ30_02980"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HPW01438.1"
                     /translation="MSDRLMATIKRTTAETDVSVSLQLSPGSIEIATGIGFFDHMLTE
                     LAFHAGWSLQISCTGDLEIDDHHTVEDVGIALGMAFSKIVPSAQGFARFGYSYAPMDE
                     ALARAVLDISNRSYCVFSGTFSMPSIGLLSTCNIAHFFQSFAANARCTLHLDILRGEN
                     DHHKAEALFKAVALAFRAALAPSPVLGSTKGEARIDSELT"
     gene            complement(23655..24680)
                     /locus_tag="PLQ30_02985"
     CDS             complement(23655..24680)
                     /locus_tag="PLQ30_02985"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPW01439.1"
                     /translation="MLRLDANEGQCLLSEEDLRAILSPEIARRYPMRELLEIPLAEKL
                     GIPSSCLLATAGADDAIDRAIRSLAGPGGLVMSTTPGFVEFLEAAQRSSARFEEIAKN
                     PFGPFPIDDICRCVKDFAPKLLILSSPDNPSGVVLSPLDVTQIAKACLEAGTIFVFDA
                     TYQEFSIHGATPIQALANPNVLVTGSFSKSKGLAGFRIGYAAATPESARIISKLSDAG
                     PPYSLSSSAIEAGRRALSLDQGVAQGFIDEVRREVHVLKSLCRKLGFLVSESEANFVL
                     LKIKNPIDLSDRLRERGILVRTWPNKPGYEELVRITVPGEPEEFKELCVSLEAAIKEY
                     NENYRMK"
     gene            complement(24698..25639)
                     /gene="hisG"
                     /locus_tag="PLQ30_02990"
     CDS             complement(24698..25639)
                     /gene="hisG"
                     /locus_tag="PLQ30_02990"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPW01440.1"
                     /translation="MAENANQKLELRLGLPKGRMQAKVIELFKDTGIPISIDDREYRP
                     RIGNGKALVFGSREIMYEAKLLKPQNIVEMLEAGSRDIGFAGEDWVRELGADLVELLD
                     TGLDPVKIVAAAPENVATAFRAYRGDLTKMISVCKRTPIIASEYEGLARAWIDKNLPG
                     AGFVHSYGATEVFPPEDADIIIDNCATGSTLRANRLEILDTLMMSSTRFYASKAAMAD
                     ATKREAAEALILLFGSVLEARRRVMLEVNVSAELLDELVAILPCLRAPTVSPLGGGDA
                     FAVKVAAPRDTLASLIPEIKRRGGTDIVATQISQLVP"
BASE COUNT         6872 a         5916 c         6236 g         6777 t
ORIGIN      
        1 cgtttgatat tttctccgaa tggagatgga cgagccgata ctgttgaatt gatacagagt
       61 agcgtatatg gggatcgatg gaatgggcaa ataatctcag agacaggaaa gattgttcgc
      121 acctgggagt ggtatccgat gctcgccgat gttttatggg atggcaaaga ccagagtggt
      181 aagcttgtat ccgatggtgt ttattattat gagctgagat cgatcgatgc ggccggcaat
      241 ttcttcatcc ttcctcgtca gcagatcata gtcgatgcag cacagaaaaa tgtcagtttc
      301 aaagttgaac ccacggcctt cagcccaaat gacgatggcg tcaaggatct aatttactta
      361 aatgtcaatg cgccaaaacc ggataccctg ctttcttact cactttccat atttgctggt
      421 tctaaagccg cggcaggtca agcgcccgta cggagctgga aaggaaaaat cgatattaag
      481 tcgcaatatg tctgggatgg tatgacggat gcgggattga aagtgccaga tgggtattat
      541 agtacgtatc ttgagcttga gtatgcaaac ggcgatctat tcaatttcgg ccctattcag
      601 atacttgtgg atacagttcc gccaaggata agcatatccg cggatccctt gcttttctct
      661 ccgaatggcg atggcatcaa ggataccata accattgcgc aggaatcagt gccaggtgat
      721 gattggtcag gcagaattcg gagtgcatcc ggggcaacag tacgaagcta tacctggaaa
      781 ggacaagcaa agaactttac ctgggatggc aaagactcga acgggaaact tgtgccaaat
      841 ggcgcttatt cctacgaagt agtcagtgta gatgctgctg gtaatagtgc ttcagcttca
      901 atcaagggta ttatggtaga tgctacaaag ccgagagtct ttgtaactgc ttcagacaat
      961 ggtatttcgc ctaatagcga tggaattcgg gatgaagtat ccttctcgct tacggtagaa
     1021 aaccgagagg gtgtcgattc gtggcgattc tcccttcttg attctaaagg tgttgaacgc
     1081 tccttctttg gtggaagtgg ttcagatgtg cctacgcggc ttgtatggga tggccgagac
     1141 ttgaatggcc aggtgataga ggatactttc acaggcaagc ttatcgttag atacatgaag
     1201 ggtgacgtag cagaggcgac aagcagcaaa atagtggtca agattgcacc tccgcaggta
     1261 gagattaagg taaagccaga atatttcagc cctgatggtg atggtgtgga tgatatgcta
     1321 accttcggta tcgtagcaga caagaatgcg ggcatcacag agtggaagct cgaagtgctc
     1381 gaaaccgcca ttgtcgaatc tgccgccttg caggaaagca agccaacgcg atctttcatg
     1441 ctctggagcg gcaagggaat gcctccttct cagatcacct ggaacggaaa atcccccaag
     1501 ggagaattag tcgaatctgc aaccgattat ccattcgaat tcacctgctg ggacgccctc
     1561 ggcaaccaga ctaagataag cggcataatt gcggtggatg ttcttgtcat acgcgatgga
     1621 gacaggctca agattagagt gccctctata gtctttaggg cgaaccatgc ggacttcatt
     1681 ggcctggata gtgagattgt agctcgaaac gaaaaagtag taactcgaat tgctcagatc
     1741 ctaggcaaat tcccggatta taggataagg gtcgaaggtc atggaaacaa tgtcggcaag
     1801 atgcttggat acagcgcatc gcgtattcag caagaagaga ttaatgagct gataccatta
     1861 tctaccgagc gagcagaggt agttcgtaag atgctggtgg aaaatggagt agatgcgcgt
     1921 cgcctgactg tggttggact tggttcgagc gaaccagttg ttcccttcac cgatgttcaa
     1981 aaccgttgga agaatagacg tgtggagttt gtgctgatca aaaatcagtg agcttttatt
     2041 gctaatcgat ataattgagg ggctgctcat ttagttgggc agcccttttt ttctgcttat
     2101 atatgccact ttcgcgccag gaaaccttgc tgcattttag cgagaacatt ttagcgagaa
     2161 taagaatata ccaaagtgct aatcagaact ctcctatgaa taggacttct ggctcaatgc
     2221 aaaaccctgt agcatcataa acaaccttct gagcgtattc tattagtttt ctcatgtcgc
     2281 gagcgctggc ttttcctata ttgacaaata tgtttgcatg ccatggagag atagctgcat
     2341 cgccgattct atagtttcgc aagccaagtt catccagtat ttttccggtt ggcttgccaa
     2401 aatcatgatt atttttgaac atagatcctg cgcttggaaa gttaaaatgc cgcttttgca
     2461 tgcgatctcg catacataag cgcatacgct cggcgatttt ttcgggatca tctggaatga
     2521 gcctaaacga tgctgcacag attattagag attctaattc gccgccgcgc atgaagggtg
     2581 agcgcttata ggaccaggac tgagcgcagg cggaaatgat tttgagtctt ccttggttat
     2641 cgataatggt aagatcttga atatgcgtgg ctatatcatc ttcataacag cgcgcattca
     2701 tatagaccga acctccaagg cttccaggca ttccatagaa attctcgagc ccagaaagac
     2761 cacaaatctg agcctcttcg cacagagtca tcaaacgaac tccacactcg gcatatagga
     2821 gcacagggtc gattctctct tcccgtttca aatgcatgat cgaagagagc agactggtat
     2881 cgatcaccgc tcccctaaca cccctgtctc ccacaagtat attagctccg cttccaagta
     2941 tgaaaaaagg tacattctca atctttagca gctccactac ctcttcaaga gccgatatgg
     3001 agcgcggcct tataaagaga tcggcaggac caccgatctt aaaagttgta tgctccttca
     3061 taggctcgtc aaaaagaatc aagcaatgat cagtattgat tttttgagcg atttgccata
     3121 ggtttttcat gtaattccag tatcttagct taaagaggca aaagcaagaa ggtccgactg
     3181 acgaaaagga gcacatatgc atgatgtcca catttttaat acgatgggtc ggaaaattca
     3241 gctttttacg cccctgcacg aggggaatgt gggattctat ggctgcggac ctaccgtcta
     3301 caattatgct cacataggaa atctcagaac atatatcttc gaagatactc ttgtccggat
     3361 gctaaggcga tttggatatc gggtgacgca tgtaatgaat atcactgacg tgggtcatct
     3421 ctccggtgat gatgatacag gcgaagacaa aatgcttaaa tcagccagag aaaaaggcca
     3481 atcagtgctt gaaattgccc aattctatac caacgctttt tttaaggata ctgagcgctt
     3541 gaatattcgg caacccgata tcgtctgcaa agcaacagac catattcagg atatgatcga
     3601 actcatcaag cgcattgagg caaatggtta cagctatttc tccggaggga atctctattt
     3661 cgatgtctcc aaattcgacc attatggcga acttgccggg cttcagctag atcagctcaa
     3721 agcaggcgca cgcgtcgagc tggatcctaa taaacgcaat cctttcgact ttgtgctttg
     3781 gtttacaaaa tcgaaattcg ataagcaagc gttaatctgg gatagcccat ggggaatagg
     3841 atatccgggc tggcatatcg aatgctcggc catgagcatg aaatatctgg gagagcaatt
     3901 cgacatacac gcgggagggg tcgatcatat agccgtgcat catacaaatg aaattgctca
     3961 atccgaagcc gcaacaggcc acagatgggt caactattgg atgcatgcgg aattcctcgt
     4021 tcttgataaa ggcaagatgt ccaaatcttc gggcagcttt cttacgcttg agtctctcat
     4081 cgataaaggc tacgaccctc tggattatcg gtatttctgt ctgggtgggc attatcgaag
     4141 ccagctctca ttttcctggg aaggactgga tcaagcgaga tcagcaaggc tctcgctact
     4201 ggatagggtt cttgcactca aagaaaaggc agtgaagcca gcatccatat cggagctagg
     4261 tcaaaaagca ctccgatatc tcactgcctt cgacgatgcc ttggccgatg acctagcaat
     4321 gccgcgtgcg ctagctcagg tatggagctt attgaaggac aatgatgttc cggctcaaga
     4381 tgctcttgcg ctcattttga atatagatga agttctgggt ctggatattg ccaaggctca
     4441 gagaagagaa gtctcggttg atacagatac cgttcaatgg atcaatagtc agatacagag
     4501 aagagcggca gccaaaaagg cacgagattt tgcgacagcc gatgcaattc gcagcgaact
     4561 tgtggcaaga ggcattcagc ttgaagatag acctgaggga acagtgtgga gattgatcga
     4621 atgattatga aaggctttct gtaacaaaag gattgatgga ttgttccaaa ataaggatga
     4681 tgggccaata tcagactaca acctcatatt tgcgcagaat gtggacctac ttctgcgcat
     4741 agccgtgcat atcaccaatg attgggaatc tgcagaagac atagtccagg atgcctttgg
     4801 taagttgatc gaaaagcaaa tgcaattccc ttcggaagag gatgctcgat tctggctcat
     4861 tcgagtggta aaaaatgggg ctataaactg gtcaaggcga aaagtgagag agttcaaggc
     4921 ctacgagcac tggtggaacg ccgaaacaac tcaggcaaag atttctgcag atgctcttga
     4981 ttcgagggat atgcaaaatc aacatgcagg aacagatcaa tccgcagatc atgaacttat
     5041 gatgaaggaa tgcgctcaag aagtgcgaga tgctctgatg aggattcccg aaaagctgcg
     5101 aatggtactc attctcaaag aatatgaagg catgaattat aaagagattg ccaaggttct
     5161 aggcattacc gaaggaaatg tcaaagttag ggctttccga gctagagaag ctcttctttc
     5221 gatattgaat gagggagggt cttatgtgtc ccgatgaaag tctgttgacg gcttatatcg
     5281 ataatgaagt accatcacca tggaaagagc ggatcgagat gcatttgtgc caatgtgagc
     5341 gatgtgcaaa tcgtgttgct caatatcgcg ctctaaaggt agcattaaag gccgccgatg
     5401 ttttggaagc ctcaaaacgg caagaagctg ccaaacggat acaggcttcg ttgaagagca
     5461 ttacagatga gtcttcgcgc ccacttcgca cagaaggtat aattgagaga tatccagtat
     5521 tttctacgtt catgtccaag cgaatatctg tgccgcttcc tattctagct gcatcgcttc
     5581 tcattatggt attctttgcg ggcttggctt ttggtatttt tggagcccat agaaccacag
     5641 gccaagcatt ggctctgagc acaaggctcc ccgaagagtc cgcatcgaac atagaaagta
     5701 tcgtgtcctt gctttcccag acagatccga accagtttgt gacaatacat gcgccgggta
     5761 atatatctca acctctggtc aatacgacac ctgtgtatgt gatctacaat tcctccggcc
     5821 gcaagccaac agtcatggcc tttccggtgg aaggggagag ataagatgaa gcgttcgact
     5881 ctcacccttg ctgcaatttc gattctgaat gttgcatggc tttttgctca gacgccctca
     5941 ataccaacag caccttcttc gattgcaata cccgattcac catcttcaaa tattcccgtt
     6001 ttccaatttc agattagaac tgctttcacc ttgacacctg ggatggtatc tgcaatccag
     6061 tcagcttcgc ttggatctca agggtcaaaa gcagctcaag gagaatccat cgagaaagca
     6121 aattctccca agtgggaaga tgcgatagtt cgtgaagtaa gcctcgcttc tccgctaggt
     6181 atcaaaattc agaacgatag attcatcgca ataatagtat tgatgcctat cgaactcata
     6241 aaaaaggaca tcaccatgct tgtccagaat cacatctata tgcgaagcca agataattcg
     6301 attcaattga atacctcggt tcatacggtg agaataccgg ttgattcggt attttattac
     6361 tatcctttgg gtggagattc caaacagggt gcacccatgg tgatcgagat tcttgtcaat
     6421 tcaaagtaag tccatgaaac agcgtaggat tattacgctt tgtattttga ttttcttagt
     6481 cgctgcagga ataatcatct atcgaaacat gcagccgagg cttttggcaa ttgatcctgt
     6541 aattgtcatg tccggcgatc ttgtcaatat tgcgggacgc aatcttggca aaaaggaagg
     6601 cgagctcctt ttggataatg ttcccctgcc ttcacaagct atactcttct ggagcccttt
     6661 tcttataaat ttccggatgc ctagcaatta tgattcggcc gttgtgcggg taaagacggg
     6721 gctgggggta tcgaatccac ttatggtggc gcttgcttcg aaagtccctt caaaggcaga
     6781 aaataatcta attcagcagc acaatccaag cattacaagc ataaaacctt cgacaaacgc
     6841 tcagatagga aaacttatcg agctcagagg cagtcatttc gggatgcctg aggaagaatc
     6901 tgcggtgctg ttttcgaaag tgcctataat ttcaacgctt gaagcgatag atgcggacaa
     6961 ttttgcaaga gtcgaatcga ttagtcttct tgtagataaa tggaatgact cttatatagc
     7021 ggcttatgtg ccggatggta ccgaaaatgg gtatgtctta gtccgaacaa gaaatggtct
     7081 ctcgaacgcc tttcctattt ctatttcgcg taatcctggc agattgtcca gaggagattc
     7141 cgtaacatat acgctcaaag atacgctcgt ggtccatatt gccgcatctc tgccagatgg
     7201 cagggtatcc ttcatcctct ccaaaccgcc tgtaacgcag taccagtcga caatggcgac
     7261 gatcgaggca ggagccgatc atctgagagc cgaaacggcg gtatggcagg aattcagact
     7321 gggtgcggcg gatgcaagac tggggagaat agaattacag aggcgattga ttatagatgt
     7381 atgcgagatc acagcagata ttaccatagg cacgctacag ccactcggaa caaagccccc
     7441 tgcttttctt agcccttatc ttgcagagga tgctctggtt ccttctacta atccttcgat
     7501 aatctcggcc gccctgtcga tgaagaaaaa ggaaaaaaat ccttttcgac aaattatgct
     7561 ggctacccag tggacattct cgaacataaa gctaaagcaa gaaaaacaga gctttggaga
     7621 tgatcctatc gatgcactca aaagcaaagc cggtggtata cgttctcttg ttttaatcaa
     7681 ttgtgctctt cttagagcat tgaatattcc agcaatcccg gtagcgggct ttcttgtgac
     7741 cgaagatatg cgcttaattc ctcattattg gtcagaatat tatcttatcg gaataggatg
     7801 gataccattc gacccagccc ttgcaatagg aatgattcct tcgggttttc gtccggtttt
     7861 ttcgaataga tccaactatt accaaggaat tgataacaag catattgcaa tggctagggg
     7921 gtatcaactt ctaccttcta ttccagcaga ggcacagaaa aaaagcaaaa tcccctggtc
     7981 acttttcgaa tatgatgaag tagctcatgg attaagttat acttcgacct ggaatccgcc
     8041 gattcttctc tcgaccacga ttcagtaatt cttacaggaa gatgggcaca cgttagtcgg
     8101 ttttttgtag tatactaata aatgccatga tacaataatt cgcgggagga gatatgtcct
     8161 acattaaatc gatggacccg gaactatatg ctgcgatgga agccgaggca agccgtcagc
     8221 gtgaaaaaat cgagctaata gcaagcgaaa actatacctc caaagctgtt atggaggctg
     8281 tcggttcggt ccttacaaac aaatacgccg aaggataccc tggaaagcgc tactatggtg
     8341 gctgcgaatt tgtcgatatt gctgaaaacc ttgcgcgcga cagagcgaga gaactgtttg
     8401 gtgcagatca tgctaatgtg cagccgcact ccggaagtca ggcaaacatg gctgttctct
     8461 ttgccgcatt aaaccctggc gacacaatta tggggatgag cttagctcac ggaggacacc
     8521 tgtcgcatgg agcgccagtc tcattctcgg gtaagttcta caaggtggtg aactatggcg
     8581 taaatcctga tactgagctt atagacttcg atcaggtggc ctttcttgct cgtcagcata
     8641 agccgaagtt gataattgcc ggcgctagtg cctattcgcg catcatcgat tttgacgcct
     8701 ttcggagaat tgcagatgag gttggggctt accttatggt cgatatggct catattgccg
     8761 gcatagtggc cgctggcaga caccccagcc ccattgcgaa tgctcatttt attacgacta
     8821 cgactcataa gaccttgcga ggtccgcgag gcggtatgat cctggtgggc agagatacgg
     8881 aaaatgatcg tggcattatg aatgctaaag gagatgcact gcgcagatgg tcggagctta
     8941 tcgacagcat ggtcatgcca ggaattcaag gtgggccatt gatgcatgtc attgctggta
     9001 aagcggttgc atttaaggaa gctcagaggc ctgagtttgt agagtatatc gatcatgtgc
     9061 tttccaacgc aaaggtgatg gcacaggctc taaccttggg aggagctaga attgtatcgg
     9121 gaggaaccga taatcacctc atgcttgtcg atctgacacc tcttggaata aatggtcgag
     9181 atgcggagag atggctcgat gaagcgagca taactgtgaa taagaattca attccttttg
     9241 ataagaaaag cgcgtttgtg acgagcggaa tacgtattgg tacgccagct attacaactc
     9301 ggggcatggg accatcagag atggagcaga tcgcttcatt tgttatggaa gtgcttagat
     9361 caaaaggcga tcctcaggtt attgcgcgaa tcaagcagga ggtcattggt ctcacctcac
     9421 gcttccctct gccataaagc tgcaatgcag aaaatagagc actcagaact ctatgagcaa
     9481 gctggtaggc aagcagcgta tcggcttctt tcgaatgcag aggaacaagg ttttttttct
     9541 tatgggatac ttacacgaca ggcatgggag cagattgtag aagagcaagg tacggttcaa
     9601 gcagactgcc gcgaagggaa gcctctaagt ctaaattcct taaggacttc gtcgccatgg
     9661 accgagcagt taaaggcaga gagcagtcca gtcatggcat taaaggagcg ctatcatctt
     9721 tcggatgtgg gagcgatgtt ggtggtagcg cttcgttatg caccggatgt cgaggccgag
     9781 gaggcggcag cccgcgcgct gcgcgggctg ccgcctctgc cacttgctcg cgacggccgg
     9841 ccagctgcga gggtggcaag gtttgcgcgg ggcaactggt atagagagat cttggatcgt
     9901 ctccgcaatt gtgctaccgc gacctttggc gcaattataa aagagggctc tgcggaaagg
     9961 cttggtctgg aagcaaagcc acctcagcga tggccgagat ttgtgaattc gatatttccg
    10021 gaaaaggcct tagccctcgc agctggcctt gggacaattg gacgaaacgg ccttcttgtc
    10081 gctaagaaga caaacaactc ggatatcttt gatgctcttg attttcaaga gcacttgata
    10141 gcaagcgact ccagtaagcc aacaacaacg aatccgccaa cttggtcttc cgctgttctg
    10201 atcggtttga tgcttcttcc attcgacttt gacatgttga aactcgaaaa aaaagatatt
    10261 tttggaggtg tggctccatc attttttgcc gtctgtggca attgcaggct ttgtatcgaa
    10321 tcctgcccaa gcagagcctt aagcttttca tcccttcctg gctttgcgcg acatctctgt
    10381 atccagaact attcatcgat cgatgggaat ctgcctgatt ttatagaaaa gtcctgggcg
    10441 gatcagcttt atggatgcga tctttgcctt gaagcctgta catactttga gccagatcaa
    10501 aaggcacata caaatcgtgg tcgaataggc ggtttttttg atgctgagtc gttggcagat
    10561 ctcagcgagg agcaaataca agctttgttc aaggcttcag ctctagatca aaaatggatt
    10621 gcacctggag cgctcaagag aaatgcggcg atagtaatcc agaagttcca taaacttagg
    10681 aattgaagcg agctgcatga caattttatg caaagatagc cggcagcata tcttcgagcc
    10741 caataattct tatcgcggtg actgagtcta ttgcagcgca gcgcaataat ggcattggcg
    10801 tcttttattg acatattatg ttaaataata tataatgccg ctgttttgtg caggagtgtg
    10861 taaaatgaag aaaatcattg cagtgttcgc aatcatatct attgcattct ttgcatgtgc
    10921 tcccaaaaag accaacattg acaatcagga tagcaaaagt gtctccaata tggctcctga
    10981 ggagatcaag gatgtggcca catggaaggc tgcctacgac gaccttatca gtgcatattc
    11041 aagcgcggtc gctcaactca acggcggcaa tctgggggca ggcgcaaaga tcgaagagct
    11101 agggaagcga gctgaggcac ttaactcagt agcagagacc attagatctg gcctctccgg
    11161 tcaggaacaa gccgattttg ctcaggtcat cgaagcttat agagaaaaat tcagccaagc
    11221 agtaaattct aattgaaatg gcccataaga tgaaaggctg agaaatagtc tgccatggta
    11281 gtccatggta gtagcaagag gtcgaatcct aaacctgggt ctgccctatg ctcatgtctc
    11341 attgtgataa gggcgggata gagtagctaa tatgaatata gatctgataa acctgcgctc
    11401 tcgtatgata agcattgtcc gcgagttttt catttcgaag gattatctcg agctagacac
    11461 gccagcgctt gcgccttatc tgatacccga atcttgcctg gaagtttttg ctacagagta
    11521 cattcatccc atttatggcc ataggcctct atatctcatt ccttcgccgg aaatatggat
    11581 gaagaaagtc atcgctaata caaataggag tgtctttcaa atctgtaagg cctggcgcaa
    11641 tgcggaatcg gtttccccta ttcataatcc tgagtttact atgcttgaat actacggcgt
    11701 gggcctttcc agcaaagaca atatcgaaat tacggaggag ctttttgaag cccttgctgg
    11761 cacagaaact ccttctcgag ttcgtccgcc ctttcgagtg atgagcatga aagaagcatg
    11821 ggctgagttt gctcatgcgg atctggatgc gcttggcgat gatgaggcca tgcgcgaagc
    11881 ttgtcgacgc cgtggattat tggtacctca aactgccgcc tgggaagacg ccttccatat
    11941 cctttttctc agcatagtcg aaccttctct tcctaaggat aagccactca ttctagacct
    12001 ttatccttcg cgtatcgaat gtcttgcagc ggatattcct ggcaccagat acaaagagcg
    12061 atgggagcta tatgtagaag gcatcgaaat cgcgaattgc tttaccgaaa tggctgatcc
    12121 tgaggctgtc gatcgatata tgcagtcgca gagcaaaaag aagagagaat cgctagtgcc
    12181 tcatattatc gatgaggcat atagctcgat ttttaaagac tttccaccat gttccggagt
    12241 tgctcttggc tttgagcgct tgatgatggt tttatgtggc aagaaatcga tcgaagacgt
    12301 gatgattttt cctttctcgt ccttttttgg ctgagacaaa tagaaaaagt tatccatgaa
    12361 atgatgtaga taaagatata agctataata aagtcctatg atgaaaacag tcgcaggagg
    12421 ctttagtctt tacatagagc aagattccaa gctagtatag tttgatttaa tgcttttgta
    12481 tcgaggaaaa cattatgatt cgtggtggtg atatcggaat cggaacttgt ctcattatca
    12541 atggcgtgcc acatattgtc gtagaacgag agtttgtcaa tccggggaag ggagccgctt
    12601 ttgctcgcgt gcgcgcgaag aatctcaaaa atggatatgt ggtgacccag acaatcaaga
    12661 ccgccgactc cgtagaagat gcccaagtcg atcttgtcga ttgtcaatat cagtatagcg
    12721 atgatgaatc ttttcatttt atgaacaacg agacattcga gcaatttgaa gtgccaatca
    12781 ccgctcatga agaccgtgct ccttatctaa aagaaggtga aacctacaca atcgtcatgt
    12841 gggaaggcga gcccatagat atcaagatcc catataaaat ggtttttacg gtagcagaga
    12901 gcgagaatta tgtgcgcggc gatacagtat ctggggcaac aaaacctgta aggaccgaga
    12961 cagggctcgt agtgcgggtg cccctcttca taaagcaagg cgagaaaatc cttatcaata
    13021 cggagacaaa cgagtacgtt gagcgcgtca acgaataagg ctgatgattc attcgattca
    13081 ttctgtcagc tcgatccgct agtcaataag agagtagact tcgcttaccg tggtactaag
    13141 ctcaaattcg aactctctta ctcgcttttt tcttcttttg atatcgatga aggaacccgc
    13201 tttcttctaa aagaaatatc taatgagcaa ggaattattc atgctcatcg ccttctcgat
    13261 gcgggctgcg gggtgggtgt aattggcata agcctagcag cctcatgccc tgagatggat
    13321 gtagtattgc gtgaccgaga tttccgagca gtcgcattat ctgcgcgaaa tgcagaacgg
    13381 aatggtcttg gtgtaaagct cttcggactg aatggacagc agcatgtcct atgccagaag
    13441 aaaaagtttg caaagataaa aataaagcag agaaaagcgg atgatctcat tgccgaaccg
    13501 ggtttgctat gcgagccgga tgagcgaggt ccctatgatg caattgtatc aaatctgccg
    13561 gcaaaagctg gagttaatgt actcgagaat tattttcatt ttgcatgcac cgagctcttg
    13621 cagcccggag gcacttttgc ctttgtcatc atagcacctt tggcagatat ggcttgtatt
    13681 tggtgcgcca atgcgggtct cgctctaata agaaccaaca gaacaaagaa tcatatggtt
    13741 gcaattgcgc aaagcccaag agaagttcaa gtaggtaagc tctcatcgaa tggctggatt
    13801 tcagcctatt tgcgttcgca tacagaaaga cagcttgcta gagcaaatct agagtgggat
    13861 ggttttcaag gcttgcccga attcgatgag ccatcttttg ttactctatg tactctctca
    13921 ttagctcaga gagtcttcgc aggtcttctc gtgcgtcggg cgcttgtagt ggagcctggc
    13981 gtaggtatca tacctatatg gataaaagaa gtcttgggaa cagaggagat tgtactgata
    14041 tcgcatgatg tgctcgcgct taccgcatcg atccataacc tagaaaagaa gggttttctt
    14101 aaagctattt ccatactcga tccaatcctt ccgattattt cgccttcagc catcgagaat
    14161 tcattgccac atgaggctct attacaagaa aaagaaggac aagtttcggt agaaaaaaag
    14221 ctcagcccag ccagcattga tgtagctgtt ttcttcctcg aagatattcc aaggtataat
    14281 atcgccagtg cttcctggtt acttacccaa aaatcactaa agcgaggggg agtagttata
    14341 ttttctggaa ctagcacaca ttgtgagcga gtctgccgag aaaaacctat gggcttttcc
    14401 aggcttccac attctgatca gaaaaaaggt tggcatgccg tagcctttag gcgagattag
    14461 gtagataagg cttgcaaact ccatcaggtt tttagaccat gagatatcta gagatgggga
    14521 tatatctttc tgcgaactta ggatatttga cttttagcaa ttctttatta gattttacaa
    14581 tatgaaaaaa gctgagcgca tgattctaga atctagaaca cccgatgaat atgtggagaa
    14641 ttctctcaaa tcgcgacttt ctcctgcaga gaaagctaaa ctagctcggt tatggatgca
    14701 gggaacaggc tttaatagag aggatattat acatgctcgc aatagaagcg aatactggcg
    14761 aaagcggaag atggcaggta caagagagcg aacaaaaagg cgaatggctc aacatgatta
    14821 ctcaaagggc aagaccatcg tatggaccag agaaaatatt gcagaatttc tatcgcttaa
    14881 tcgcaaggat gcgtatggac gctatctata tcgcgattgg gaactagctg ctctttttag
    14941 cactagcata ccctcgattc aatacctaag acgcaaattc aacaaagtca gcaaaatgct
    15001 cggtcctggt gcaaggcgag ataaagtcat cgattatatg acctgttccg agctagtgct
    15061 tcaacgcgga ggcccaaaag ccctcaaaag caagcgctaa taacctctac ttgtcagaat
    15121 aagggtttgc gcgatctaga acttggcgaa ttactctatt atcggggctg ttgagcgatg
    15181 gatcatcctc gatgattcta aaagcatcat ttcttgcttt cttgagaaga tcaaaatcgc
    15241 tgcgcatatc agcaatgcgc aattgcagat ctcctgattg ctctgcgccg aacatatttc
    15301 ccggtcctct tatcttcaag tcctcttccg caatagcaaa gctatcgttg gtctcataga
    15361 gagctttcaa cctcattcgc ccttcttccg taatatcttt tgaatagaca aaaaaacaat
    15421 agccctgaag atcacctctt ccaatacgtc ccctcaactg atgcagagcc gacagtccga
    15481 aacgctccgc atgctcgaca accattaccg tcgcattagg cacatcgact ccgacttcga
    15541 cgacagtggt tgcgaccaag acagatattt caccttcaga gaaccttgac atcacatcca
    15601 tctttgcatc gtcgttcaag cgagaatgca aaattccaac cttgaactca ggaaagatct
    15661 tcttggaaag gtgttctgcc atcgattccg catctctaag gccaagtcta tccgattcac
    15721 tgataaccgg atatacgaaa taggcttgtt ggccttcttt gagttttttg cgtgtaaact
    15781 cgtatacgga atctcttcgg gattcgttcg ccagatgggt ggtgactggc ttgcggccag
    15841 gtggtaggtg ccgaatcgta gatacttcca tgtcaccaaa aaaggtaaga gcaagactcc
    15901 ttggtatggg ggtggcggtc atcatgagaa aatctggcac atggcctttc ttgtagagag
    15961 ctattctttg gagcactcca aagcgctgtt gttcatctat gatgatcagc tcgagattct
    16021 tgaaagaaac atcatcggag aagagagcat gtgtgcctat agcgatatct atatcaccac
    16081 ttttcagagc ttctagtagt tgaggccgaa ctgcgctcga tatcgagcca gttacaaatg
    16141 caaggcgtac acccaaaggc tcgacaagat gcgcggctat ggttgcatgt tggcgtgcga
    16201 ggagttcagt aggagccatg attgccacct gtcctcctcg ctcgactgca tatagtgctg
    16261 caagcagagc caccattgtc tttcctgatc cgacatctcc ctgcagaagt ctggccatag
    16321 gaaaaggttt gtgcatgtcc tggattattt ctttgaggac caattcttga tcctcagtaa
    16381 gcgtatacgg aagacgttcg cgaagctgtc ttgcaagtac gccgggaaca gcgatgcgat
    16441 ttatggtctt tgtctgtctg gattgaatcc taagagcaat tccaagctga aagtaaaaga
    16501 gctcttcgta ggcgagccta tcatgagcag acttgagcga atcccatgat gaaggaaaat
    16561 gaattgagcg gatggcttcg gacttggatg gaaagttgta gagttcacgc agagttcctg
    16621 gaagttcatc ttcaagatga agcgcgaatt tttgaagtgc tagggctatc atccttctaa
    16681 gagttgtctg atttagcctt ccagtgagcg gataaatagg cgatagagtc cgttctggga
    16741 caaagttatc ctgtagggtt tttagctcaa aggcagaaga ctggatttcc ccatacttaa
    16801 actggaattt gccatagact tggattgtag aaccaacagg ggccatctgc tcgagaaaag
    16861 gcctgttgaa gcagagaagg gcggcctcgg aagattcatc cgcaattatg atcttcagtg
    16921 tgcgcattct tccaaaacca aaccattcat gacgaatgac cttagcctta gagtagacct
    16981 tgccttcaga aaaacgcgat aaaggaagga tttttgttct gtcttcgtaa tctctcggca
    17041 aatggagcaa aagatccgca atagtatgaa tattcagatt agcaagtgaa cgcaaggttg
    17101 caggtcctgt acctctgaga atgctgatat cttgtgtcaa ttctcttaaa aacactggtt
    17161 ttagtataca aagagtctta ttccagtgct actatatgca tgatgaaaag tgataaagga
    17221 ggaaatcgat gcaaaaagag acagctcgcc ttatagagct tgatcgagaa cacacgctta
    17281 tgtctcatat cggcgctgtc cttggatggg atcaggagac ctatatgcct tccaaagcga
    17341 tcgaggaacg tgcctctcag attgcacttc tcgaagggct tgctcaccaa aaagcggtaa
    17401 atccagagat agatgagctg cttgcgacgc tagaagagaa acatgatctt tctctagacg
    17461 agagagcata tgtccgcaag atgcggcgag attatgacag agaaacgaag ctgcctgagg
    17521 catttgtcac cgaatacgct aaaaccacta gcattgcaca agcaaaatgg gctgaagcga
    17581 agaagaacaa cgattttagg gcattcgagc ctcaccttga gaaaattgtg gatctttcgc
    17641 ggagtttagc aggatatcta aatccgaatg caaagcccta tgatgtgctc ctcgacctat
    17701 atgagcccgg aaatacgcag caatcggttg catctatttt ttctacaatg aaggcttacc
    17761 tggtcgatat tctcgacaag attcgcggca gacctcaaat agaagacagg tgtgtggggc
    17821 ggtatgtaga ttctgagact caagagcgaa taagcttata tttcatgaat gttctgcatt
    17881 acgagatgga tcgcggaagg ctggatgtct ctgcccatcc ttttaccacg agcctcggtg
    17941 cagatgatat acgcattacg acgcgctata ttgaagacta ttttccttca agtcttttta
    18001 gcacgatcca tgaatcaggt catgcgcttt acgatatggg tattgatcct gatcctgagt
    18061 ttagggggac aaaactggct gaagctgtat cgatggcagt tcatgagtcg caatccaggc
    18121 tgtgggaaaa tatcgttggt cgatccaggg tattttggga gaggaatttc ttagctttga
    18181 gtgctcttct gggtaaggct ggtgaggatc ttgactttaa gagctttatt gaatcgatca
    18241 atagagtgag tccctcgctt atcagaaccg aagcagatga agtcacatat gggttgcaca
    18301 taattgcgcg ttttgagcta gagtcggcgc tattagatgg gaatctgcgt gttacggatg
    18361 tgcctggagc atggagagag aagtacaagg aactgctggg tcttgaggta tcaaatgatc
    18421 gagagggctg tcttcaggat gtgcattggt caataggata tttcggatat ttcccgagct
    18481 atgcacttgg gaatctctat gccgcacagt tctgggcaaa actcaaggaa gaagtacctg
    18541 atatagaagc tcgaatagca agtggtgatg ttctctccgt gctaacatgg cttcgggcta
    18601 atgtgcattg tcatggttca cgctatatgc caggtgaact cgtcgaaaaa atcactggat
    18661 cggctctcga tccagcgtgg tttgagagat atttgcgaga aaaatactcg agcatatatg
    18721 ggttctgagt tttaggagtc gggaccttta tttttgtgtt tgtgcacgcg cgcaagtgcg
    18781 gcttgtgcgt gtgcctcgag gccttccagg cgggcaaaag cagcggtatc ctctgcgaga
    18841 agctccggat tttcgatggc aagccatgtc ctggctcgca agaagcaaag gaccgacaga
    18901 ccagctgcaa atctcgatgc tcctcctgtt ggcagagtgt ggttgggtcc tgcgccatag
    18961 tctccgaata cttcggctga tcctatgccc agaaagatag aacccgcgtt ctttatctgt
    19021 ttcataaggc ccacaggatc cgaagttgcg atctcaaggt gttcgggcgc acagcgattt
    19081 gctccttcga caattcgcaa gagatcttgt tccacacaaa gaaagccatt cgaaagggct
    19141 cgataagcta tgctttgatt tggttcagga agatcttcga gtctagtcat gagagatttt
    19201 tcaatttcaa gggctatagt ctcttcgctt acaatggctg cgggcattgc atctggggag
    19261 tgctctgcct gggctagaag atctgcggcg atcatgtcgg ggtctgcggt cttatcggct
    19321 ataatgagga gttcggaggg gcctgccggt gcttcagtgc ccacaatgcc aaagagttgg
    19381 cgttttgctg ccataacata tttcccgcca ggccccacga tgatgtcgca ccttggaata
    19441 catatgccaa aagccagggc agcaatagca tgggcgcctc ctacctgaag gaatccatca
    19501 gcacccgcca cccaggcggc ggctaaggtt tcattgctag gtcttggtcc agcgcaccag
    19561 acttcttcga cgcctgcaac ttttgccgga acaacagtca tgattgcgct cgagggcagt
    19621 ggaaaggctc cgcccggaac atagcagcct acgcgcgcca cgggcacgag atcgtgcccg
    19681 tggcggccga ccagcgcgcc tttttcgcct ctacctgtat ttctttttgc tgccatttcc
    19741 cctgttgcaa tatttagcgt tgtgggccgg tttgaatcgt ttgaatcttn nnnnnnnnnn
    19801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gggggccttc tggcctctgg cgaatacatc
    19921 tgagatttca tctttatcgg ctctattcgt atcggcagct ctagtggtgt aagacaggcg
    19981 cgctgggcgg ccgcaaaggc cgctacgcgg cctttggcgc gctccattag ctcggtggtt
    20041 ttcttaggca acttatcata agcagccttc aactcatccc gagagattac aagaggcgcc
    20101 ccgactggaa gcccatctaa tctctctgcc catcgcctga gttctggctc cttgccagct
    20161 cggatagcat cgatagcttg cttggcaacg gcttcaattt caggatcgaa aagcctcacc
    20221 gaaggttctg caatatcatc aatcgcaatt ctttttaatg gatgcttttc cattcgcttt
    20281 ttccttcaag cccttcgaat tctggctttg catttccccc acgccgcgta acacgcaagg
    20341 aacgacgctc gagttcttcc tcgatatccg aaagaccgcc gcccatgcta attgtcttta
    20401 ccaaggcaaa atagagtaca tcagcagcct cagatatcgt ctcttgtaga gatttagact
    20461 gtataagctc ttgcacttcc tcgcgaagct tggatgcgag aagggcttca tcggcaaata
    20521 gcctgcgcgt ataagagccc tggggtgctt ctttcaagcg tctcttgata gtacgatcga
    20581 gtttttccag accataaccg ttatcaaagc aggttcgtct gccaagatgg cagaagcctt
    20641 cgccattctg acgcaccgta aagcggattg tatctctatc gcaatcgagc tctgcgcgca
    20701 gaaggatttg gtcgtttccg gatgattctc cttttaccca gaggcccctc gatctagaat
    20761 gatatactcc cttgccgcaa ttcaaagcaa gttttagact ctccttgctc gaatgcacaa
    20821 gcccaagtgc ttttcccgac tcgtcgcata caacagtagg ccatagtcca tcagctctgt
    20881 cagtaataag gggtgcagca aatgcctcag caagagaaag gtctcctaaa acaagcgaag
    20941 tacccgcctg gaggtctgca cccagcctat ccagagtcgc aatatcagca atgcctcgtt
    21001 cgccaccagc ggcccctccg gcaaatgtga atttgctatc aggggcaaga gcgattagct
    21061 tccttgctct ttcgatatcg ataccacact ggtcgccctc ggtttctata aaggtaatga
    21121 gaaagccgga cacatagggt gcaagagcta tgatcgattc ctcgatagaa ccttcccttt
    21181 gctttgacca tccttccacc atgatattcc cttctttact atccagggct gctataaccc
    21241 gctcccgagg caagctggaa aggaattcag gtgtggccga agttccaata ataatcgagc
    21301 gggctcctcc attgagataa tccatagcaa gatcaatcgt gcgaatgcct ccacctacgc
    21361 gcgctcgata tattctgcac aaagaaagaa tgagttctcg gttgcttcca actccttttg
    21421 ccgcgtcgag gtctactacg gcgaactctc caacccgacc atagcgttcg gcgagctcca
    21481 aagggtttcc agcatccaaa ggtggctgct ttcctcctct aagctgtact gctcggccat
    21541 tcgttatgtc gatactgggc acgatcataa tcttacctcc acatcatgtg ctttcaagaa
    21601 ttcctttatc tctcgaattg agatttctcc gcgatgaaaa atgcccgcag ccaggagcgc
    21661 tgatgctcca cttaaatatc cctccagaaa atgctccggc tttgaagccc ctcccgaggc
    21721 gacaactggc acgagcactg cccgcgcgac aagtcctatg agcctgcact catatccaag
    21781 gcttgtacca tctcgatcta tcgaggttag aagaatctca ccggcacctc gaccgctagc
    21841 ctcctgagcc caggcgactg catcgaaacc ggttttgcga gagcctgcat cgattttcac
    21901 ttcatatcct gaaggcattg tagaattctc tgccacatcg atagccacaa ccacacactg
    21961 tacaccaaag cgcgaagcaa gttcatcgat aatcaaggga ttgcgcaccg ctgcgctgtt
    22021 cacagagata cgatccgctc ctgcttcaag caggcggcat gcatcctcga tgcttccgat
    22081 tccacctccc accatgattg gaatggagag cacttgccgt atacgtgcca cttcttgagc
    22141 catggtcttt ctgccctcca gagtggcgct aatatcgaga aaggcgagct cgtccgctcc
    22201 ttgcctttcg tacaaaagag cgcgttcaag cgggtcccct gaatcgacaa ggtttagaaa
    22261 tcttgttcct ttgactacgc gtccatcctt gaagtccagg caagggatta ttctcactgc
    22321 aagtccgttc atacatttct cccaattctg tttagatcga tccagtgcga aaaaagtctt
    22381 gtaccaaagg acccggaaag ttccggatga aactgacaga gaaggagtgt gggttttgca
    22441 tcttgccagg cttccagcgc cgcaacaaat ggctctccat atcttgcact cgcaatggct
    22501 acttgatcaa aaacattttg cgcaaagggg gcacctttag agccttttat cgttgtttta
    22561 taagtgtagg ccacttctcc cacccggtaa ctattagcaa aatagaccca gcctgattcg
    22621 agaagatgcg cagagctctc agattcaatt ttgttccacc ctagctgagg caagggcaga
    22681 cctcctgcca gcctttttac ggtaccattg aaaatacaaa gccccttcga gcccggagct
    22741 tcttcgctcg cttcgaaaaa gagttgcata ccaagacata tacccgccgt tggaagacct
    22801 tgttctacac gtctacatat cgcctcgtcg agaccatcct gggatagttt ctccatagct
    22861 ggtccgaaag ctcctactcc cggaaggata acaacttgtc cctcaagtac atcgatcatc
    22921 atatcgctca ctacaggctc gaaacctagc cgttttgcaa atgcttctac ggaggcaaga
    22981 tttgctgctc ctgtcttgat aattataagt ttttttcttt ccttcatgct catgttagct
    23041 ccgaatctat gcgtgcttct ccttttgtgc ttcctaatac tgggcttggt gctagagcag
    23101 ctctgaacgc cagagcaacg gccttaaaaa gcgcttcagc cttatggtgg tcgttttctc
    23161 cgcgaagaat gtcgaggtga agtgtacatc gtgcattcgc cgcaaaactc tgaaagaagt
    23221 gggcaatatt acaggtcgaa agcaaaccta tagatggcat agaaaacgtt ccggagaaga
    23281 cacagtagct tctgttagaa atatccaata cagcacgcgc cagagcctca tccattggag
    23341 cataggaata tccaaagcgt gcaaagccct gtgccgaagg aactattttc gaaaacgcca
    23401 tgcccagagc tatgcctaca tcctctaccg tgtgatggtc atcgatttcc aggtctcctg
    23461 tgcatgaaat ctgcaagctc cagccagcat ggaatgcaag ctcagtaagc atatggtcaa
    23521 aaaatcctat gcctgttgcg atttcgatgg atcctggcga taattgcaga ctcacgctga
    23581 catctgtctc ggccgttgtt ctttttatag tggccattag tcgatcgctc atagacgctc
    23641 cttaatccaa ggtattattt catgcggtaa ttttcattat attcttttat agctgcttcg
    23701 agagaaacac acagctcctt gaattcttca ggttctcccg gaacggtgat tctaacaagc
    23761 tcttcataac ctggcttatt aggccatgta cgtacgagaa ttcctcgttc gcgtaaccta
    23821 tctgagagat caataggatt tttgatcttc aacagaacga aattagcttc gctctccgat
    23881 accaaaaagc ctagttttct gcaaagcgat ttcaaaacat gtacctcccg ccgaacttca
    23941 tcgataaagc cttgagcaac tccttgatca agagaaagcg ctcttctgcc tgcttcgatc
    24001 gctgagctcg aaagcgaata aggaggacca gcatccgaaa gtttcgagat aatccttgct
    24061 gattcgggag ttgccgcagc ataacctatg cgaaagcctg caaggccctt ggatttcgaa
    24121 aagctaccag ttacgagcac atttgggttg gctaaagcct gaataggcgt tgctccatgt
    24181 atcgaaaact cctgataggt cgcatcgaaa acgaagattg ttcctgcctc gagacaggcc
    24241 ttggctattt gtgtcacatc taggggagac agcacaactc ctgaagggtt atcggggctc
    24301 gagagtatga gcagttttgg agcgaagtct tttacacacc tgcatatatc gtcgatcgga
    24361 aaaggtccga atggattttt tgcaatttcc tcgaatctag cggatgatcg ctgggcagct
    24421 tccaggaatt cgacgaaacc gggagtggtg ctcattacaa ggcctcctgg ccctgcaagg
    24481 ctccgtattg ctcgatcgat tgcatcgtcg gctccggctg tggcgagcag gcaggatgaa
    24541 ggtataccga gtttctcggc taagggtatt tccagaagtt ccctcatagg gtagcgtcgg
    24601 gctatctctg ggctcagaat cgctctgaga tcttcttctg agagaaggca ttgaccttcg
    24661 ttagcatcta atctaagcat tttcttctcc ttttgcctta gggcacaagc tgcgatatct
    24721 gagtggctac aatatcggtg cctccacgtc gctttatctc tgggataagg cttgcgagtg
    24781 tatcgcgagg agccgcaact ttaaccgcaa aggcatctcc ccctccgaga ggggacacag
    24841 tgggtgctcg tagacaagga agaatggcga caagttcatc gagaagttct gctgatacat
    24901 tcacttcgag cataacacga cgcctcgctt cgagcacaga accgaagaga agaataagcg
    24961 cttccgctgc ttcgcgcttg gttgcatctg ccattgctgc ctttgaggca tagaatcttg
    25021 ttgaactcat catcagggta tccagtattt cgagtctgtt agcccgcagg gtagagcctg
    25081 ttgcacagtt gtcgattatg atatctgcat cctctggagg gaaaacttct gttgctccat
    25141 aagaatgcac aaatcccgcg ccaggtaagt ttttgtcgat ccaagccctt gctagtcctt
    25201 cgtactcgga agcgatgata ggagtccttt tgcagacgga tatcattttt gtaagatctc
    25261 ctctgtaagc gcgaaaggct gttgccacat tttcgggagc cgcggcaact atctttactg
    25321 gatcgagacc ggtatccaaa agctcgacca aatcggctcc aagttcgcgt acccaatctt
    25381 cacctgcaaa gccgatatcg cggcttccag cctctagcat ctcgacgatg ttctggggtt
    25441 ttagaagctt tgcttcgtac attatctctc tagatccaaa gaccagggct tttccgtttc
    25501 cgattcgtgg acgatattct cgatcatcga tggatatagg tatgcctgta tccttgaaaa
    25561 gctcgatgac ttttgcctgc atcctgcctt ttggaaggcc gagcctcaat tccagctttt
    25621 gatttgcatt ttctgccatt ttttctcctc ctccttgcaa tcttttatca ggcaaggaaa
    25681 aaataagccg gccccgttgg gagccggctc tggcaaaatc gacaaaaaag ccggctccct
    25741 ggagccggct tcgattcaat attaagctgc gtttattccc gcgcctaacc acggctccat
    25801 gtgaaaaagc cgtgatgatg atgaagatta agcgctttct taacagatgt ccctttcata
    25861 ttttcgtaat attgcagttt cttccaaaag agtcaagagg g
//
LOCUS       DAOMZL010000020        24827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_1677_length_24827_cov_15.953900, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000020 DAOMZL010000000
VERSION     DAOMZL010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 24827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24827
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1677_length_24827_cov_15.953900"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            397..1020
                     /locus_tag="PLQ30_02995"
     CDS             397..1020
                     /locus_tag="PLQ30_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPW01441.1"
                     /translation="MDANTNRSSKLLSLCVFCGSSMGKRLSYAEAAEKLGRTLAEQGM
                     VLVYGGGDVGLMGIVAEAVMKNGGKAIGIIPRRLYELVDQQEVSELLVVQDMHERKAL
                     MEKKADAFLCMPGGIGTMEELFEVWSWRYIGYHQKPVALLNIEHYYDHLLEMLKHMVE
                     EGFLHKEIYEDLIVSDDIGYLLDALRNKATTSSAAPFLKKPERRNHN"
     gene            complement(1027..2466)
                     /locus_tag="PLQ30_03000"
     CDS             complement(1027..2466)
                     /locus_tag="PLQ30_03000"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HPW01442.1"
                     /translation="MYKNALFTDFYELTMAQGYWKREMTMPVVFDYFFRKHPFNGGYS
                     VFAGLATLIEALQEFSFSQDDLEYLSTLGLFEDEFLHFLSSFRFKGTVYAAREGEIIF
                     PQEPLARIEADLISAQIVEGLVLNSLNFQSLIATKTTRIWNASHRGSIMEFGLRRAQG
                     ADGALSASRAAFIGGAMGTSNTLAGKEYGISVLGTMAHSWVMSYPSELDAFNAYAEIY
                     PQNTTFLIDTYNSLESGIINAIEVGKKLKEKGYSFGVRLDSGDIDYLSRAIRARLDEA
                     GFEDAKIVVSNELDEEIIESLVDDGAPIDIWGVGTNLVTGGNEAAFSGVYKLAGIDPE
                     DRLRPVMKFSDNPEKSTNPGVKNLWRLYDEKGAARLDLISCQDEQIQEGIEYIVHHPS
                     ADWRRLKITPARVEPLLFKVMEKGNRTATLPDIRQIQAFMKERIQAFDSTYLRLLNPH
                     IYKVSITDRLYDLKVFLINSFLKHKLSRN"
     gene            complement(2472..3131)
                     /gene="pncA"
                     /locus_tag="PLQ30_03005"
     CDS             complement(2472..3131)
                     /gene="pncA"
                     /locus_tag="PLQ30_03005"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HPW01443.1"
                     /translation="MDDALIVVDIQNDFCPGGVLAVPEGDSIIPKVNELLSAYSLSIL
                     TQDWHPAMHCSFASSKGLPPFSLDMGTRPPSVLWPDHCVAGSQGADFHPALQSWRARY
                     IFRKGTRKELDSYSAFLENDGVTSTGLAGLLSSLGVDKVIVCGLATDYCVKATALDAR
                     RLGFEVVVVEDAIRGIGASPDDIENAQVQMREVGCVFAQSGDLMASYTISSISPSKGI
                     I"
     gene            complement(3230..4756)
                     /locus_tag="PLQ30_03010"
     CDS             complement(3230..4756)
                     /locus_tag="PLQ30_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01444.1"
                     /translation="MKRVRAVLALILHFLSCSMLAWTAETSENPLSGNLSFSASSKKT
                     KLTLFLRHGSDALLNLSIPLSIDKNWLENIAFTGKARLAGYDMTAGKLAVKDSASFLI
                     APVLASAQGRSALSISDSGKSSDPIGISIAASRISLLAGKGDPYAFASMQYLCAQEGL
                     LFSIGFGTLLDIESSIDILDRLDPWVSMGIGYTKPSVSIMVRSHLYPDFEKRSENLLD
                     IPWLKKIGMRLDMNIFAGSQKLQGFLYIESGHFLSALGKVASYDAMAQIGYDTDISFL
                     RILKSLNVSLCAFSNQGLVEPVDAKTPSFGTLPDLWTMQYWPDGGKIGLTIESQKAAF
                     SLFRTALNLKTSISGALAINTKSWSNNIALDLMLLRAASSEMFKINGTLQGSHSIGRK
                     LIEIGEEESSESIGNEELGSIEETEDQAASPDHGSSLSPAFDKLLLSGRLKINPFSFL
                     MSVNLPLHNRNSRATSGRIGMSLAKRQLLMELSVSMQVEPESRQLQFKSFSCYAKIPL
                     "
     gene            4926..5156
                     /locus_tag="PLQ30_03015"
     CDS             4926..5156
                     /locus_tag="PLQ30_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HPW01445.1"
                     /translation="MSVTIYTTPSCGYCKMAKEWLQQKGISFTEYNVAADMRRAEEMV
                     RKSGQMGVPVIDIDGKIIVGFNKPEIEKYLHH"
     gene            5233..6156
                     /locus_tag="PLQ30_03020"
     CDS             5233..6156
                     /locus_tag="PLQ30_03020"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01446.1"
                     /translation="MKQFKSAVILNRSIAPNLMHLAFEWSEDLLPPLPGQFFTFLPAM
                     VESSAGIILRRPLAFAGFESKKHAAKSSGYQSAIAHSIYQIRGPCTRALALQRPGSHI
                     DIIASLGNSFPYPDGDEKAIIAGGGIGIGPMLFLAASLAENGTIAQSYIENNSKSLET
                     SISGKEIQLVFGFRNTLFVPKFKEPTYDIPILNTWEKLLSGAFMATDDGSEGFHGTVL
                     DALECLKKDNKSLEIWHIYGCGPGAMIAELAAFAERNNFPAHLSAEQWMACGIGACQG
                     CVLPARKGGYLRVCADGPVFEADAIDWEACL"
     gene            6153..7133
                     /locus_tag="PLQ30_03025"
     CDS             6153..7133
                     /locus_tag="PLQ30_03025"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPW01447.1"
                     /translation="MINSIKDLNDFSASFEPDLSVKIGSLTMTNPVGVASGILGYGEE
                     YKELVQIDELGALYTKTVTLEPREGNPAPRLVETPMGLINSIGLANPGLECFLREKLP
                     LLRSHCCAIIVNIAGSTEDEYAMLVDRIEEELTLSGPMSKTGVDAYEINISCPNVLKG
                     GMTFGIDPIIVERLTGSLRRRTARPLIAKLSPNVTDITEIARAAEEGGADAISCINTV
                     VGMVIDTDRMKPAIAKGLGGLSGPAIRPIGVAATYRVSHAVSIPVIGVGGITRAADAI
                     QYLLAGAQAVQIGTALFSNPLAPQEILNGITEWMKKHGVLRVHDINSMLK"
     gene            complement(7182..7718)
                     /locus_tag="PLQ30_03030"
     CDS             complement(7182..7718)
                     /locus_tag="PLQ30_03030"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HPW01448.1"
                     /translation="MGIEKEFIPERLIVGVLSSDAEAEIAALDAMIGLHGPLCFQSDK
                     EPFLWTRYYCPEMGEDILRSYWAFGELIDPSRLSIIKRATNSIEQRLARNGCRLVNLD
                     PGMLGTARFCLATTKDRSHRIPLSDGIYAELTLIFEHGEFKALPWTYPDWASVPVRNM
                     MTALRAGLLGDLRHLHFL"
     gene            7760..9364
                     /locus_tag="PLQ30_03035"
     CDS             7760..9364
                     /locus_tag="PLQ30_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HPW01449.1"
                     /translation="MNSRFFLVVSLLSIAFSPLAAQMQEREFVTVYGSQIPELDPQKA
                     IFSNEAQIHSAMYEGLFTYDPQTLEPLRAMAQSWERSVDKKLYRFTIREDAIWSDGSP
                     ITAQDFVRSWFRMLDINAEYATFFDIIEGAKQYRMGLDKNPEHVGIKAVSANVLEIAL
                     VRPVAYLTRLLCHHSFSVIHPSMIDKEDWRSAIPYPVNGPYKPVSITETELVLEKNEA
                     YWDSENVSIPMLRMMFTDDDELATSLFNTGYVHWLDGPGNYEKVLLQTAIQVFPIYST
                     HYWFFNCRSEPWNDKRVRRALALLLPWDSIRSSEFYMLPAKTLVLPLPGYSKVKGIEK
                     SDSTEAMKLLEEAGYPKGQGLPEIIIAFADYERPNLIANTLKTEWEKALDVEVTLQPM
                     QASSYYDSIGNRSDAWSFTVAHETWIGDFADPEAFLQMWASDAPLNIAGYNDSAYMDF
                     LIQSYDASDNDRMNILAKAETELLQGAACLPIYHNFAASVIDIDFIQGWYQNALDIHP
                     YKYLRFGTPSISPNVAKASSSYISLR"
     gene            9386..10501
                     /locus_tag="PLQ30_03040"
     CDS             9386..10501
                     /locus_tag="PLQ30_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPW01450.1"
                     /translation="MTNIDETGEFLKSLGFPRLNIHATARHHDFISSSKRILVIGPMG
                     SGKTEFAARIWRDSRVALKKKEVVKRLTSTANVDRRNVFVVRSALDKSRFPDYPEDAL
                     AYRGGYEQCGNRIGAIKDSFGLEVLIAENPEVGTWIIDEAAFFDERLAYLTKDEAESR
                     GLCFIFPTLLLNFRREIFNQTARLLLDTATDVVPLTAYCEHEECLRDSLYTYRYYLVD
                     NQECPALYFDPLIIIGGDRTKHDGREPDYCTRCDDHHYLPAKEYTFFTLKPFGELAAR
                     GNISPLFAELAAFQGKIEESRLYSSILGRCSEGIEREMLMNSLKVPLIAERALIYLYA
                     EQNLLSEEQMRFFIQKLDLDRDYLSKRLADNRRPLAL"
     gene            complement(10565..11809)
                     /locus_tag="PLQ30_03045"
     CDS             complement(10565..11809)
                     /locus_tag="PLQ30_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HPW01451.1"
                     /translation="MNTEMLDSRLFYLDPWLKEADVVVKTIEPVTDRRSFVFGVMLSG
                     TIFYPEGGGQPADFGFIEDKRVIDVQEIDGEIWHYIELQDENDRDSLKPGSTVHLRLD
                     WPRRLYHMQQHTGQHLLSAVLEEEYGIHTLSFHLGTEYCTIDVSAKDPIELPLMQIET
                     QVEAWIGRDAAVLVHYCPPEDISTFKLRKNPPADEAVIRIVEIQGYDCSPCGGTHLDR
                     TGQVRALKVLSLERYKGHVRMYFASGAHAIELMSKAYDDNRAAATMLGSSVGDISTRL
                     SEVLEKSSSTEKKAKRILVQYAEAEARLASIHAGPTGILQFTLEKEDVDWGTMLCKAA
                     VSLGRPAIASCLNDSTVIIQVPAKARIPGLSKIFSEIMANLGGKGGGGEAFYRASFAS
                     ADIAKQFAIKAREVLERYQTQC"
     gene            complement(11806..14685)
                     /locus_tag="PLQ30_03050"
     CDS             complement(11806..14685)
                     /locus_tag="PLQ30_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW01452.1"
                     /translation="MYLKSLEIFGFKSFADRIRIEFSKGISALVGPNGCGKSNIVDSI
                     KWVVGEQSAKSLRAETMEDVIFNGTETRRPLSVAEVAITISNDEGVLPLDVPEIEVKR
                     RIYRSGENEYFINSKPARLKEIRELFWDTGIGKSAYSVMEQGRIDQVLSSKPEDRRYL
                     FEEAAGITKHRVRAREAELKLAKTEEHLRQIEAIIGEVKQSYENLRVQSDKTIKYRQL
                     KDKIFETELDLYLVRLRQYIREQDRVCNLFDQKKKEREALVQRIESMSQSMSDALDML
                     NKLEARLIEMQKLIYGLAVEKNGKERQKALLTERLREIKSIIEQLEARDRAIASKIES
                     LQEEEGDKEAELAGYRIRIREIEANIHTFDENISAAALLLKANEESIRHNVEESKEIA
                     QKTRLLHEKLDTITERIAELVDERLRKSESQIEQKQNLEAQIRQKIKEISISTSSWAN
                     KLQDYTRLAPSLTAQERFRELEGLVNEMRALSSLSSDLEKDFLHYVEIEPGFLNELIA
                     PEGIMTQKRRIDAEILNQRERIGRIEAENQELTLKNTDLAKKIETYRKTLEEARLEKT
                     KIQAQMDAAKEALSVLKREIAGQEAYHREVAGELLDQHRRHEELKEQIELFQEELAEM
                     EQKGRHLTEEMSELDKSISRATEDLGDKRKESSELEQNLQAIQRELEDLHTAKAQRET
                     EIRNLKENFVDLYSRDLLAFESRMYEIRSPVSEIKEKLVSLKASLSSLGSVNLMAPEE
                     FEPVRQRFEFLSGQYDDLVKARADLVRITNEIRTESAKLFVETYNKIKKNFHNIFRRL
                     FGGGRAELRLSDTDHVLESGIEIFAQPPGKRLENISLLSGGERTLTAIALLFATYMVK
                     PSPFCFLDEIDAALDEANIVQLVNLLREFGTMSQFIVITHNKKTVASADTLLGITMEE
                     SGVSKAIAIRIQNENGVVHPVYVLDQAFEEEEVDYEEGRQLEVNR"
     gene            complement(14771..15424)
                     /locus_tag="PLQ30_03055"
     CDS             complement(14771..15424)
                     /locus_tag="PLQ30_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPW01453.1"
                     /translation="MDDDILTIEEVAKYLRVSERTVYDWAQKGEIPSGKIGTVWRFKK
                     SEIERWVNERLSSNRPVSLINSVKIQNIISLDRIIFLDYPSKRDALIKLAENLSTAPQ
                     IKNKEELISEIIRREELMSTAIGKGIAIPHVRLNSVTDLVVSIGISRCDIVDFQTLDD
                     VPVRLLFMIAAAFNQHAYYLQTLSFFSARLKVVELRDGLLNCKTTQEAYDLLIQQDR"
     gene            complement(15508..16368)
                     /locus_tag="PLQ30_03060"
     CDS             complement(15508..16368)
                     /locus_tag="PLQ30_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HPW01454.1"
                     /translation="MAKRIDSVRKKASGSLEVAVSGGFVFCFDEVDAQLLDYSLKTHP
                     SDLPFSGKYFLDVHSPAEHSVDALARHSSAVHLIAADSIEFSESAIAGHYGPKSILLF
                     QDESIDEDSLLLLGRLNDLHRARSDALLIVSHNEQASRQLYLKLLKRGYEPEIANTCV
                     RWTCAEHYVDDRRYVRMLFKLHCVKKGQGPTRMQALAWPRIGLFESPQTILADGMAAI
                     PEDDLMEAIKKNIESYQRKPSTFSRLDLNSNSSEKRTFLRSFLRRQGFPYQSIERFLE
                     TLVEKEHKND"
     gene            complement(16375..16773)
                     /gene="rpsI"
                     /locus_tag="PLQ30_03065"
     CDS             complement(16375..16773)
                     /gene="rpsI"
                     /locus_tag="PLQ30_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPW01455.1"
                     /translation="MVKNINLGIGTGRRKCAVARVFLREGSGKIFINEKDLKDYFAVD
                     GLIAQVRKPFLVTDSENKFDAYITVVGGGLSGQAGACSHGLARALAQIDPANHISLKA
                     NGLLTRDPRMVERKKYGQRGARRRFQFSKR"
     gene            complement(16791..17219)
                     /gene="rplM"
                     /locus_tag="PLQ30_03070"
     CDS             complement(16791..17219)
                     /gene="rplM"
                     /locus_tag="PLQ30_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPW01456.1"
                     /translation="MKTAFFTAATAERKWYVIDAAGKPLGRVAAKVASILRGKNKPTF
                     TPSQETGDYVIIINADKVAITGRKRQNKTYYHHTGYPGGIKDFSFDQLIARNPVSPME
                     IAIKGMLPKGPLGRKLFKNAKVYAGPDHPHLAQNPIAIDI"
     gene            complement(17349..17597)
                     /locus_tag="PLQ30_03075"
     CDS             complement(17349..17597)
                     /locus_tag="PLQ30_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01457.1"
                     /translation="MTALLVGIGLIVLAVLMVIPGILGWWSDVLAFLRGALPVIAFLI
                     GALAVFIGIADIKDKIEEKKEEEKEKAEEKNKSENSEN"
     gene            17828..19684
                     /locus_tag="PLQ30_03080"
     CDS             17828..19684
                     /locus_tag="PLQ30_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HPW01458.1"
                     /translation="MIKTIDGDERKVRNKDLAMVHAGPIHSFPIAQHSKEVNEALDLL
                     KEENPEGVPIVSWNEFAELAWSKVEAKHIVIAWHMLLENPAIEMLDNGFRIRSKEEQQ
                     KLIEKERKKKEITETRAAFVEAFGLAWKKNDPSFVDKDQNFKPFVDELSRFARGFAVD
                     SPIARDLGIRLTPQAVHEALLATGFWEPFINPWPDRNGCILSMPSEPKNIRERSELTL
                     DRLDLRYLSSFAIDNPWSKDPDDAISIEGNRVWIHVSDPVSAIAPISTEDEDACARAS
                     TLYLPEKTIPMLHHSLIEAFGLGILPESKALSFGITLQKNGKIDQISIVPSIIKVARL
                     TYEEADVLLETNEVLQNLNEIVEARKKLRQDQGSVDIVFPEVSIRAIDGNVEFLPVPA
                     TRSARLVQELMILAGEAAARWAYAAGLAFPYVSQEPPISAESAGCGAGLSESLAENYA
                     RRKVMRTAIVSSTCGAHAGLGLSFYSQVTSPLRRYQDLLAHYQIHAELARQKSSNILE
                     EGIPREAQIGLSREMLDQRLYLYGLQAAKNRQAERDSRLHWAMVYLSKHRDWKGQAIV
                     LEVDRGHAHIYIPSFGIEMLIHISRNVKPDTNITIALRRVSIPELTATFDIV"
     gene            complement(19711..20577)
                     /locus_tag="PLQ30_03085"
     CDS             complement(19711..20577)
                     /locus_tag="PLQ30_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPW01459.1"
                     /translation="MKSSKIRRPSSFLAEILTIILFVLFLFPFFLVLINSAKTSFEVT
                     QYPLAWPKKWETIVDNIIKIWTSESVCYPSSLLSSAIITAVSLILINVFSAMAGWALV
                     RTKTKVSSFIFFIFVASMVIPFQIVMFPLLSWFRTVTVATGIRLLRTYQGIILSYVGF
                     GAPLSIFMFHGFIKSIPLELEEAATIDGCHRHQIFFRIIFPILKPIQATVLVLNGIWI
                     WNDYLLPLLVLGKGNDVMTVPLAVSNFAGAFVKQWDLILTAILMAMIPVIVFFLFAQK
                     YIVKGMVAGAIK"
     gene            complement(20574..21494)
                     /locus_tag="PLQ30_03090"
     CDS             complement(20574..21494)
                     /locus_tag="PLQ30_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPW01460.1"
                     /translation="MTKKMESALVFWVFLLPVLVAFIMVMVIPFILGSYYAFTNWTSS
                     ARVDSVLRFVGFENFINSLKDPSFLYSFGITFLYTILNMIVINIVAFVLALLVTGDLK
                     FKNVYRVGFFLPNLIGGIILGYIWQFIFNNALPSLGSIISAFDFLSDPANLMLSKPNT
                     AIAAMIIVGTWQYAGYIMMIYVAAIENIPQELIEASRMDGATPWIRLKSITIPLCAQA
                     FTVTMFLTLVNSFKQFDVNVSLTSGGPSTMLMGQPIFGTELLALNIYNTAFISNKLSE
                     AQARAFIFFLILAIISIIQVNVNKKKEIEL"
     gene            complement(21621..22853)
                     /locus_tag="PLQ30_03095"
     CDS             complement(21621..22853)
                     /locus_tag="PLQ30_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPW01461.1"
                     /translation="MKRFLLAFMALLTVTAAFGAGKLTLMQNKPEIDAQLKAYAAAWG
                     QKNGATVVIKSIGGTSGGMGPQLKADYAAGDMPDIFAFDGLEAYKEWEGIILDLSNEA
                     WVSKTSVPFKYNGKVYGFPVAIEGWGLAYNSDLLAKAGIDPKTLNNFDAYKKAFEKLD
                     SMKKDLGINSVVSMAASIEMGWVTAHHNFNSLLSNGLPYGDLSVTNDLLAGKVDAKRL
                     QEYADWVELLFKYSDKAILLTGNYDAQVGAFATGKAVFLHQGNWVDPNLKDANATFKM
                     AFAPHGSMKKVTDGIFVSAPSYYAINKESKNIALAKKFLNDLVVTPEGNNYMVKEAGM
                     IPAFSNITLGPEGQLSKSVQQWSAAGKVYSWNQYYFSGDFRDKVLTPIYNQFAAGSIS
                     KAQFIDLMTKAFKDNAKK"
     gene            complement(23298..24440)
                     /locus_tag="PLQ30_03100"
     CDS             complement(23298..24440)
                     /locus_tag="PLQ30_03100"
                     /inference="COORDINATES: protein motif:HMM:NF015754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCCL domain-containing protein"
                     /protein_id="HPW01462.1"
                     /translation="MQRKENLRQLFVLFICIIGVFALVPVFGQSSPNLRTIAGLQPGS
                     VLFLSIKGDVSGALSLWGTDIYTLDSNVAAAAVHAGVIANGQTGIVAVVICDGLSSYS
                     GSLRNGVQSNSWGSYSLSFRFVGLAQASGSLAQATPSAPPATQTPPTQLKPPTPSTPP
                     LPQPPVASRNLYAFDDPYVLKRNGNFKPGTTFYVQVTGKVSGADVWGTDIYTLDSDLC
                     AAAVHAGILSDGQTGIVKVTILPGQQSYVGSTRHNITSMNYGNYGMSYSLEPVTQSYD
                     QVVWMILDPGTADYIPNVRVGKSYVVWVTGMADQRKVWGTDIYTSDSYIANAAVHAGI
                     LKPGESAPVIVHVLEGQPNYEGSTRNGITTKAYGSYERSYTLEPAR"
BASE COUNT         6853 a         5761 c         5369 g         6844 t
ORIGIN      
        1 ggtcttaagg tgggctcatt tgtttaatgt tttaaagcgg agtatttcct ccatctagca
       61 ttatcaaatt cccaatggag agcttactcg ttaccatgga atatttggcg cgctaaaata
      121 cattgaaatt ccatgcgcgg cgtgtagaat atatgaagaa caggcaggtt ccaattcagg
      181 ggaaatactg atattcaagg ctattctatg ataaagacgt ggggttcgat tccccacctc
      241 cagtgtaatg aaagatgttc ttccgtatcc gttcgattca aggccttatc tcacagtgtg
      301 cctgttcttt ataagagttt caatatattg acttattttc agaaagccct tatttgtaca
      361 aatctgtatg gatgatggtg gggagataaa gacataatgg atgcaaatac caatagatct
      421 tcaaaacttt tgagtctctg tgttttctgc ggctcttcca tggggaaacg cctatcgtac
      481 gccgaggccg cagaaaaact cggacgcaca ttggcggagc aagggatggt cctcgtctat
      541 ggcggaggag atgttgggct tatgggcatt gtggctgaag cagtcatgaa aaatggagga
      601 aaagcaattg gtatcattcc tagacggctg tatgaactcg tagaccaaca ggaggtctca
      661 gaactgctcg tcgtacagga tatgcacgag cgcaaagctc tcatggaaaa aaaagccgat
      721 gcttttctat gcatgccggg cggtatagga acaatggagg aacttttcga ggtgtggtcc
      781 tggcgatata tcggttatca tcaaaagcct gtagccttac taaatataga gcattattac
      841 gatcatctat tggaaatgct gaagcacatg gtcgaagaag gctttctaca taaagaaatc
      901 tatgaagatc tcatagttag cgatgacata gggtatttac tcgacgccct tcgaaataaa
      961 gccaccacat ccagcgcagc accattctta aaaaaaccag agcgcagaaa tcacaattga
     1021 ggaaattcaa tttcgcgata gcttatgttt tagaaaggaa ttgataagaa ataccttcag
     1081 atcataaagt ctatcggtaa tagaaacctt gtaaatgtgt gggttgagaa ggcgcaaata
     1141 tgtcgaatca aaggcctgta tcctctcttt catgaatgcc tggatctgcc ttatatcggg
     1201 aagcgttgct gttcgattcc ctttttccat cactttgaaa agcagaggtt ccactctagc
     1261 cggagtgatt ttcaaacggc gccagtcggc gctgggatgg tgcacaatat actcgattcc
     1321 ttcctgaatt tgctcatcct gacaagaaat aagatcgagt cgagcagcgc ctttttcgtc
     1381 gtataagcgc caaagattct tgacaccggg attggtcgat ttttctggat tatccgagaa
     1441 tttcataaca ggccgcagcc tatcctctgg atctatgccg gcgagtttat agacaccgga
     1501 aaacgcagct tcatttccac ctgtcacaag attcgtacct acaccccaga tatcgatcgg
     1561 agcgccatcg tctacgaggc tttctattat ctcttcgtca agctcattcg atacgacgat
     1621 ttttgcatct tcaaagccag cttcatcgag tctagctcga atagccctgg agaggtaatc
     1681 gatgtctccc gaatcgagac gcactccgaa gctataaccc ttttctttca gttttttccc
     1741 aacttcgatc gcgtttataa tgccagattc aagagaatta taggtgtcta taagaaaggt
     1801 tgtgttctgt ggatagattt ctgcgtaagc attgaatgca tcgagttcgc tcggataaga
     1861 catcacccag gaatgcgcca ttgttccaag caccgagatt ccgtactctt ttcccgcaag
     1921 tgtgtttgag gtccccattg caccaccgat aaaagctgcc ctgcttgcag agagcgctcc
     1981 atcagctccc tgagctcttc tcaaaccgaa ttccataatc gaaccgcggt gtgaagcatt
     2041 ccagattctc gtcgtctttg tagcgattaa gctctggaaa ttcaacgaat tgagtaccag
     2101 accctcgacg atctgcgcag agattaggtc agcctcaata cgggcaagtg gctcttgggg
     2161 gaagattatt tcaccttcgc gagccgcata tacagtgccc ttaaatctaa aagaagataa
     2221 aaagtgaaga aattcatcct cgaagagacc caaggtcgag aggtattcca gatcatcctg
     2281 agaaaaagaa aattcctgga gggcttcgat aagggtggcc aaaccggcaa atacggaata
     2341 gccaccattg aatggatgtt tacgaaagaa ataatcgaag accacaggca ttgtcatctc
     2401 acgcttccag tagccttggg ccattgtcaa ttcatagaaa tccgtaaaga gggcgttctt
     2461 ataaatacaa ttcatataat tcctttcgat ggagaaatac tagatatagt ataggatgcc
     2521 atcaggtcgc cggattgcgc aaagacgcat cccacttcac gcatctgtac ttgggcgttt
     2581 tctatatcgt ccggagacgc tccaattcct ctaatagcat cttctaccac tacaacctca
     2641 aatcctaatc ttcttgcatc cagagccgtt gcttttacac aataatcggt tgccagtccg
     2701 caaacaataa ctttatctac tccaagcgaa gacaaaagcc ccgcgagccc cgtagatgta
     2761 acgccatcat tctcgaggaa ggccgaatag ctatctagtt cttttctcgt tccttttcta
     2821 aagatatagc gagctctcca ggattgaagc gctggatgaa aatcggctcc ctggcttcct
     2881 gctacgcaat gatctggcca gagcaccgaa ggaggccttg ttcccatatc gagtgaaaat
     2941 ggaggaagtc cttttgagga agcaaaagaa cagtgcatag ccggatgcca atcctgagtt
     3001 agaatgctaa gagaatatgc agagagcagt tcatttacct ttggaattat ggaatctcct
     3061 tccggaacag caaggactcc accggggcaa aagtcattct gaatgtccac gacaatcaga
     3121 gcatcatcca tctcaattcc tcccttaaaa gatgtaaaaa ggcttgtatt atacaaaatg
     3181 gtatcgcaca gagagaaaat tgaaaactac cctttgcatt aaaataatct tatagcggaa
     3241 tcttggcata acaactaaag gatttaaact gcagctgtct cgattctggc tcgacctgca
     3301 tagataccga gagctccata agcaactgac gctttgctaa ggacattccg attctcccag
     3361 atgtagctct tgaattccta ttgtggagag gcagattcac ggacattaag aatgagaatg
     3421 ggtttatctt taatcgtccg gatagcagaa gtttgtcgaa tgcagggctt aagcttgaac
     3481 catgatctgg gctcgcagcc tggtcttcgg tttcttcgat tgaccccaat tcttcattgc
     3541 caatagattc cgagctttct tcttctccta tttctatcaa tttacgcccg atcgaatggc
     3601 tgccctgaag agttccattg attttgaaca tttcagagga agcggctctc aaaagcataa
     3661 gatctaaagc tatattattg gaccaagatt tggtatttat tgccaaagcc ccagaaatgc
     3721 tggtcttcaa gttcaaagcc gtccgaaata acgaaaaggc tgctttctga ctttcgatgg
     3781 tcaatccgat tttcccaccg tcaggccaat attgcatggt ccatagatct ggcaaagtgc
     3841 cgaatgatgg ggtttttgca tccacaggct cgaccagacc ttggttggaa aatgcgcaaa
     3901 ggctaacatt aaggctcttc aagattcgca aaaaggatat gtctgtgtca tagccgatct
     3961 gagccatagc atcgtagctc gctacctttc caagcgcaga tagaaaatgt ccagactcta
     4021 tataaagaaa accctgcaat ttttgactgc cagcaaatat attcatgtct aacctcatgc
     4081 cgattttctt caaccaggga atatccaaca gattttcgct tctcttttca aaatctggat
     4141 ataaatgaga tctgaccatg atagatactg aaggttttgt atagccaatt cccatcgata
     4201 cccatggatc aagacgatcc agtatatcta tcgagctctc gatatcgaga agcgttccaa
     4261 acccgattga gaaaagcaag ccttcttgtg cacatagata ttgcattgaa gcaaaagcat
     4321 aggggtctcc ttttcctgcc aaaagtgata ttcttgaagc tgcgatcgaa atgccaattg
     4381 gatcgctcga ttttccgcta tcgctgatcg atagcgctga tcttccttga gcagatgcca
     4441 ggacaggtgc aatcaaaaaa ctagccgaat cttttaccgc aagctttcct gcggtcatat
     4501 cataccctgc aagccttgcc ttacccgtaa atgctatatt ctcgagccaa ttcttatcga
     4561 tcgatagagg aatcgataaa ttcagaagag catcagaacc atgtcttaag aatagagtta
     4621 gtttggtttt tttggatgaa gcactaaatg agagatttcc agacagagga ttttcagatg
     4681 tctcggctgt ccaggctagc atcgagcagc taagaaaatg cagaatcaaa gctaaaactg
     4741 ctcttactct tttcatatga taaagacgct agttgaagac gagtatgctt taaaaacatt
     4801 ctggcatttt tttcgtttgg gacaattgac acaaatgtca atatatatta tttttactat
     4861 gaaatgagat tactatatct attgctatga atcaaaactg ccatatgcaa tagggagaaa
     4921 aatatatgtc tgttactatt tatacgacgc caagctgcgg ctattgcaaa atggccaaag
     4981 aatggctaca gcaaaagggc atctctttta ccgaatacaa tgtcgctgcc gacatgcgac
     5041 gcgcggagga gatggtcaga aaatcgggcc agatgggcgt accggttatc gatatagacg
     5101 gcaaaatcat agttggcttc aacaagcccg aaattgagaa atatctacat cactgagttg
     5161 aactcctgca ttatttggaa catggtatca aatccagaat tcttctgtta gaattccgct
     5221 caggtgaaga ctttgaagca attcaaatcg gcggtaatcc tgaatagatc catcgctcca
     5281 aatttgatgc atttggcctt tgaatggtca gaagatctac tgccgccatt accaggccag
     5341 ttctttactt ttttgcctgc aatggtggaa tcgagtgcgg gaatcattct gagaaggcct
     5401 ttggcctttg caggtttcga atccaaaaaa catgccgcaa aatcatccgg ctatcagagc
     5461 gctatcgctc actcgattta tcagatcaga gggccttgta ctagagctct tgcccttcaa
     5521 cggcctggca gccatatcga tatcattgca tcgctaggaa atagctttcc ttatcccgat
     5581 ggagatgaaa aggcaataat tgcgggtggc ggaataggaa tcgggccaat gctatttctg
     5641 gccgcatcgc tggcagaaaa tggaaccatt gcacaaagct atattgaaaa taattcgaaa
     5701 tcactcgaaa cttccataag cggaaaagaa atacaactcg tcttcggatt tcgcaatacg
     5761 ctttttgttc ctaagttcaa agaaccaaca tacgatattc caatattaaa tacttgggag
     5821 aaactcttat ccggggcttt tatggcaacg gacgatggat ctgaaggctt ccatggaaca
     5881 gtgctagatg ccctggaatg tcttaaaaag gacaataaat cgctcgagat ttggcatata
     5941 tatggttgcg gtcctggggc gatgattgca gaattggcgg cttttgcgga acgaaataac
     6001 tttccagccc atctttctgc tgaacaatgg atggcttgcg gcataggagc ttgtcagggt
     6061 tgtgtgcttc ctgctcgaaa gggaggctat cttcgtgtct gcgcagatgg ccctgttttc
     6121 gaagccgatg ccatcgattg ggaggcttgc ctttgataaa ctcgataaag gacctaaatg
     6181 atttctctgc ctccttcgag ccagatttat ccgtaaaaat cggttctctt actatgacaa
     6241 accccgtggg agtcgcttct ggtatacttg gatatggcga agaatataaa gagcttgtcc
     6301 agatcgatga attgggtgct ctatatacaa aaaccgtaac tcttgaaccg agagaaggca
     6361 atcccgcgcc gagacttgtt gaaacaccga tgggactaat caattctatc ggtctagcta
     6421 atcctggcct cgagtgtttt ctcagagaaa aactgccact cttgaggagt cactgctgtg
     6481 cgatcatagt caacattgca ggctcgaccg aagatgaata cgccatgctg gtcgaccgta
     6541 ttgaggaaga actgacacta tctggaccca tgtcaaaaac aggtgtcgat gcctatgaaa
     6601 taaatatctc ttgtcccaat gtactcaagg gaggtatgac ttttggaatc gaccctatta
     6661 tagtagaacg actgactggc tcgttgagaa ggcgcactgc cagaccgcta attgcaaaac
     6721 tgagccctaa tgttacggat ataacggaaa tagctcgcgc agcagaagaa ggtggggctg
     6781 acgcaatatc ttgtataaac acggttgttg gcatggtcat agatacagat cgcatgaagc
     6841 cagctattgc caagggctta ggcggtctct cgggtcctgc gattcgtccc attggagtag
     6901 ctgcgaccta tcgcgtcagc catgcggtat ccattcccgt gataggtgtt ggcggcatca
     6961 ctcgcgctgc cgatgcgatt cagtatttgc tcgcaggtgc tcaagcggtg cagattggaa
     7021 ccgcactttt ttcaaatccc ctggccccgc aagaaatact caatggaatc accgaatgga
     7081 tgaaaaagca cggtgtgttg agagtgcacg acatcaattc tatgctgaag tgagattatg
     7141 ttttggagaa ttgctttttc tcggaacata tcggaacata ttcaaagaaa atgcagatgc
     7201 cttaaatcgc caagaagccc ggctcgaagc gctgtcatca tgtttctcac tggcaccgat
     7261 gcccaatcag gataggtcca tggaagcgcc ttgaattctc cgtgctcaaa gatcaaagtc
     7321 aactccgcat agattccatc cgaaagcgga atacggtgcg atcggtcttt ggttgtggcc
     7381 aagcagaatc gagcggttcc aagcatgcct ggatcgagat tgaccaatct acaaccattc
     7441 cttgcaagcc tctgctctat ggaatttgta gcgcgcttta ttatcgagag tctggatgga
     7501 tctatcaact ctccgaaagc ccagtaagaa cgcaagatat cctctcccat ttccggacag
     7561 taatatcttg tccacaaaaa aggctctttg tctgattgga agcaaagcgg tccgtgcaac
     7621 cctatcattg cgtcgagagc ggctatttcc gcttctgcat cagaagaaag cactcccaca
     7681 atgagcctct ccggaataaa ttctttttcg attcccatgt ttattgccat ccgaactatt
     7741 atatcgcata atcaagtgta tgaattcaag gttttttcta gttgtatctt tattgtccat
     7801 agctttttcc cctcttgctg cccagatgca ggaaagggaa tttgtgacag tatatggctc
     7861 tcaaatacct gagctggatc cccagaaggc tatcttctcg aatgaagctc agattcacag
     7921 cgctatgtat gaggggctct ttacctatga tccacagact ctcgaaccat tgcgtgccat
     7981 ggcccagagc tgggagcgtt cggtggataa gaagctatat cgctttacca ttcgagagga
     8041 tgctatctgg tccgatggct cacccattac cgctcaagac ttcgttcgat cgtggtttag
     8101 aatgctcgac ataaatgctg aatatgcaac tttcttcgat ataatcgaag gtgcaaaaca
     8161 atatcgcatg ggcttggata aaaatccgga gcatgtcgga attaaggcag tttcggctaa
     8221 tgtgctcgaa atcgcactgg ttcgccctgt cgcctacctt acaaggctac tctgccatca
     8281 ttccttctct gtaatccatc cttcgatgat agataaagaa gattggagaa gtgcaattcc
     8341 ctatcctgtt aatggtcctt ataagccggt cagcattaca gaaacagagc tagtccttga
     8401 aaagaacgaa gcttattggg actccgaaaa tgtcagtatt ccaatgcttc gaatgatgtt
     8461 tacagacgat gatgaactcg ctacatcgct tttcaatacg gggtatgttc attggctcga
     8521 cggacccgga aattatgaaa aagtgctcct tcaaacagcg attcaggttt tccctatata
     8581 ttcgactcat tactggtttt tcaactgccg ttcagagccc tggaatgaca agagggtaag
     8641 gcgagctctt gctcttcttc taccttggga tagtatccgc tcaagcgaat tctatatgct
     8701 gcctgccaag actctggtgc ttcctctgcc tggttactcg aaggtcaaag gcatagaaaa
     8761 atccgatagc acagaagcca tgaagcttct cgaagaagcc ggatacccaa agggtcaagg
     8821 ccttcccgaa ataatcatag catttgcaga ttatgaaaga ccgaatctta ttgccaatac
     8881 tcttaagacc gaatgggaaa aagcgctcga tgtggaagtc acactgcagc caatgcaagc
     8941 ttcgtcctat tatgatagca tcggtaatag gtcggatgct tggtccttta cggtagcgca
     9001 tgaaacgtgg atcggcgact tcgcagatcc ggaagctttc cttcagatgt gggctagcga
     9061 tgctcctcta aatatagcag gttataatga ttctgcttac atggactttc tgatccagtc
     9121 ctacgatgct agcgataacg atcgcatgaa tattcttgcc aaagccgaaa cggaattgct
     9181 ccaaggcgct gcttgtttgc cgatatatca taactttgcg gcatctgtca tcgatatcga
     9241 ctttatccag ggatggtatc aaaacgccct cgatatacac ccttataaat acctgcgctt
     9301 tggtactccc agcataagcc cgaatgtagc aaaggcatct agctcataca tttccttgag
     9361 atgaattgaa acaccaggaa aaactatgac caacatagat gaaacaggcg aatttcttaa
     9421 gagccttggt tttccgcggc taaacatcca tgcaacggct cggcaccacg atttcatttc
     9481 atcgagcaag cgaattcttg ttattggccc aatgggctca ggcaaaaccg aatttgccgc
     9541 gcgcatctgg cgagacagcc gtgtggcgct aaaaaaaaag gaagtggtaa aaagactaac
     9601 aagtacagcc aatgtcgatc gaagaaatgt atttgtggtt cgctctgcct tagataagag
     9661 tcgtttcccc gattatccag aggatgcatt ggcatatagg ggtggttacg aacaatgtgg
     9721 aaataggatt ggcgccataa aagactcctt tgggctcgaa gtgcttatag cagagaaccc
     9781 tgaagtgggc acctggatca tcgacgaagc agcattcttc gacgaacgtt tagcatatct
     9841 cactaaggat gaagctgaat cgagagggct ttgttttatc ttccctaccc tgttactaaa
     9901 ttttcgtcgc gagattttca atcaaaccgc tcgactattg ctcgataccg cgacagatgt
     9961 ggtgccactt acagcttact gcgaacacga agaatgcctg agggattctc tctacaccta
    10021 tcgatattat ctggtggata atcaggagtg ccctgcccta tacttcgatc ctctcatcat
    10081 aataggagga gaccgaacca agcacgatgg tcgtgaacca gattattgca ctagatgcga
    10141 cgatcatcat tatttgcctg caaaagagta tacttttttc actctcaagc cttttggcga
    10201 acttgccgcc cgcggaaaca tctcgcccct tttcgccgag ttggctgctt ttcagggaaa
    10261 aatcgaagaa tcgagactct attcatctat tcttggtcga tgctctgaag ggatagaacg
    10321 tgaaatgctg atgaattctc ttaaggtacc tctaatcgcc gaacgggccc ttatctatct
    10381 ctatgccgag caaaacctat tatccgaaga gcagatgcgc ttttttatcc agaagctaga
    10441 tctcgatagg gattatctca gtaagagact ggccgacaat agaaggcctt tagcactctg
    10501 aaggcatagg taggtgcccg agaggttgat ggcttaaggc ttcaaaaagc aataaagact
    10561 agccctaaca ttgagtctga tatctttcca gtacttctct tgcctttatg gcgaattgtt
    10621 ttgctatatc tgctgaagca aagcttgccc gataaaaagc ctctccccct cctccctttc
    10681 cacccaaatt cgccataatt tcggaaaaaa tcttcgagag cccagggatt cgagcctttg
    10741 ccgggacctg tatgataaca gtggaatcat taaggcaaga agctattgct ggtcttccaa
    10801 gcgatactgc cgctttgcaa agcatagtac cccaatctac atcttctttt tccagcgtaa
    10861 actgaagtat acctgtgggt cctgcatgga tcgatgcaag cctggcctcg gcttcggcat
    10921 attgtactaa gattcttttt gcctttttct cagttgagga agatttttcc aagacttcac
    10981 tcagtcgagt cgaaatatcg ccaacgcttg atccaagcat agtggcagca gctctattgt
    11041 catcataagc cttggacatg agctctatgg catgtgcgcc cgatgcaaaa tacatgcgta
    11101 catggccctt atagcgctct aacgagagca ctttgagagc gcgaacttgc cctgtccgat
    11161 caagatgtgt tcctccgcat ggagagcaat cataaccttg aatctcgacg atacgaataa
    11221 cggcttcgtc tgctggagga ttcttcctga gcttaaaagt tgaaatgtct tccggaggac
    11281 aataatgtac cagaactgcc gcatctcgcc ctatccaggc ctctacctgg gtttcgatct
    11341 gcataagggg aagctcaatc ggatcttttg ccgaaacatc gatcgtgcaa tactccgttc
    11401 caagatgaaa agaaagcgta tgaatgccat attcctcttc gagcaccgca gagagcaaat
    11461 gctgtcctgt gtgctgttgc atgtggtaaa gacgccttgg ccaatcgagt ctcaaatgaa
    11521 cggtcgaacc aggctttaga ctgtctctat cattctcatc ttgcagctca atataatgcc
    11581 aaatctctcc atctatttcc tgaacatcga taactctctt atcttcgata aaaccgaaat
    11641 ccgctggctg ccctccgccc tccgggtaga atattgtccc gcttagcata acgccaaaaa
    11701 caaaagacct cctatcggtt acgggttcta tagtctttac aacaacatcc gcttccttca
    11761 gccatggatc gagatagaac aatcttgaat ccagcatttc ggtattcatc tatttacctc
    11821 tagctgccta ccttcttcat aatctacttc ctcttcttcg aaagcctggt cgagaacata
    11881 gactggatgc acaacaccat tctcattttg gatacggatt gcgattgcct tgctgactcc
    11941 ggattcttcc atcgttatac cgagcaaggt gtcggcgctg gccactgtct tcttattatg
    12001 ggttatcaca atgaactgac tcattgtgcc gaattctctc agaaggttaa caagctgtac
    12061 aatattggct tcatcgaggg cggcatctat ctcgtccaaa aaacagaagg gcgagggctt
    12121 gaccatatat gtagcaaaaa gaagcgctat ggctgttaat gtcctctctc ctccggaaag
    12181 cagggagatg ttttcgagtc tcttccccgg aggttgagca aagatttcta tgccgctttc
    12241 caggacatgg tccgtatctg aaaggcgcaa ctctgctctt cctccaccaa acagccttcg
    12301 aaatatattg tgaaaattct tctttatttt attataggtt tccacaaaga gctttgcgct
    12361 ttctgttctt atctcattgg ttatccttac aaggtctgcc ctcgctttta cgaggtcgtc
    12421 atattgtcca gaaagaaatt caaatcgctg tcgtactggc tcgaattcct ctggagccat
    12481 aagattcacg gaacctagcg acgacagaga agccttcaag gatacaagtt tttcctttat
    12541 ttcggaaaca ggagagcgaa tttcatacat gcggctttcg aaggcgagca aatctctcga
    12601 atagagatct acaaaattct ccttgaggtt gcgaatctct gtttccctct gcgcttttgc
    12661 tgtatgcagg tcttcgagct cccgttggat ggcttgcaaa ttctgctcga gttcgcttga
    12721 ctccttgcgc ttgtctccaa gatcttcagt cgctcgacta atggatttgt cgagctccga
    12781 catctcctct gttagatgtc tgcccttttg ctccatctcc gcaagctctt cctggaacaa
    12841 ttctatttgc tctttgagct cttcgtgtcg tctatgctga tctaagagct ccccggcaac
    12901 ttcgcgatgg taagcttcct ggcctgcaat ttctctcttt agtaccgaaa gagcttcttt
    12961 tgcagcgtcc atctgtgctt gaatcttcgt cttctccagt ctggcctctt ccagtgtctt
    13021 gcgataggtc tcgatttttt ttgccaggtc tgtatttttt agcgtcagct cttgattctc
    13081 ggcttcgatt cttccaatgc gctctctttg attcaaaatt tccgcatcga tgcggcgctt
    13141 ttgagtcata atgccttcgg gagctataag ctcatttaag aatccaggtt ctatttccac
    13201 ataatgaaga aagtcctttt cgagatcgga ggaaagagaa gacaaggctc gcatttcatt
    13261 gaccaggcct tcgagctcgc gaaaacgctc ttgggcagtc aaagaagggg ctaatctggt
    13321 ataatcctgt aacttgtttg cccaagaaga tgtggagatc gatatttctt ttatcttttg
    13381 ccgtatttgc gcctcgaggt tctgtttctg ctctatctgc gactcggatt tgcgaagtcg
    13441 ctcatccaca agctctgcaa ttctctcggt gatagtatcc agtttctcat gtagaagcct
    13501 tgtcttttgg gcaatttcct tggattcctc gacattgtgc cttatgcttt cctcatttgc
    13561 ctttagcaat agagcggccg cagagatatt ctcatcgaaa gtatgaatat tagcttcaat
    13621 ttctcgaatt ctaattctat agccagcaag ttctgcttct ttatcgccct cttcttcctg
    13681 gagcgattct attttagagg ctatggctcg atctctggcc tcgagttgtt caattattga
    13741 cttgatttct ctaagcctct ctgtcaagag ggctttttgc ctctctttgc catttttttc
    13801 gacagcaaga ccatagatca gtttctgcat ctcgatcagc cttgcttcga gcttgttcaa
    13861 catatctaga gcatcggaca tcgattgact catagactca atgcgctgga caagtgcttc
    13921 gcgctctttc ttcttttgat caaaaaggtt gcagactcgg tcctgctcgc gtatatattg
    13981 gcgcaaacgg accaaataga gatctaattc ggtctcgaat atcttatcct taagttgcct
    14041 atacttgatt gtcttgtcgg attggactct gaggttttcg taagattgtt tgacttctcc
    14101 gattatggct tcgatttggc gaagatgctc ctctgttttg gcaagcttga gttcagcttc
    14161 gcgggcgcgg actctgtgct tcgttatgcc cgcagcctct tcaaacagat atcgccggtc
    14221 ttcaggtttc gaggatagga cctgatctat gcgcccttgc tccatcacag aataggctga
    14281 ctttccaatg ccagtatccc agaaaagctc cctaatctcc ttcagtcttg ctggcttgct
    14341 gtttatgaag tattcatttt cccctgagcg atatatcctt cgttttacct ctatttcggg
    14401 cacatctaga ggtagcacac cttcatcatt gcttattgta atcgctacct ctgcgacaga
    14461 aagaggcctt cttgtttcgg tgccgttgaa tataacatct tccatcgtct ccgctcggag
    14521 gctcttcgca gactgttcgc ctaccaccca ttttatcgag tctacaatat tggatttgcc
    14581 gcaaccatta ggacctacca gagccgagat tccttttgaa aactcgatgc gtattcggtc
    14641 cgcaaaggat ttgaatccaa atatttcaag gctttttaag tacacagaac gatcccttcg
    14701 tagccgaata aaaagcaccc acagaaacgc atatgcgcac tgcaggtgct ctcacagaag
    14761 cagcaaagcg ctatcgatct tgctgaataa gcaaatcata tgcttcttgc gtggtcttgc
    14821 agtttagaag cccatctcgc agctcaacta ccttgagtct tgcagagaaa aacgacagcg
    14881 tttgcaaata atatgcatgc tggttaaatg cggcggcaat cataaacaat agccgcactg
    14941 gcacatcatc gagcgtctga aaatcgacta tatcgcaacg agaaatgcct atagaaacca
    15001 cgaggtccgt aaccgaattc agccgcacgt ggggaatggc gatgcctttt ccgatcgctg
    15061 tactcatgag ttcttcgcgt cggataattt ccgagatcaa ttcttcttta ttctttattt
    15121 ggggtgcagt cgatagattt tctgctaatt tgataagtgc atctcgcttg gagggataat
    15181 ccagaaaaat gatccgatca agggaaatga tattctggat ttttacgctg ttgatgagcg
    15241 aaacaggcct gttcgaagaa aggcgctcgt tcacccatcg ctctatttca ctttttttaa
    15301 aacgccagac agtgccaatt tttccggacg gtatttcgcc tttctgagcc cagtcgtaaa
    15361 ctgttcgttc ggaaacccgc aagtacttcg ctacttcttc aatagtaagg atgtcgtcat
    15421 ccattcgata tgcgcctccg acttgaattc tctgacatat tattgaatct atttgtaagg
    15481 tctgtcaagt attggaaaat agtattttta atcatttttg tgttccttct ctaccaaggt
    15541 ttcaagaaac ctctcgattg actgatatgg aaaaccctgt cttcttaaaa atgaacgcaa
    15601 aaaagttcgc ttttcagaag aattggaatt tagatctagg cgcgaaaatg ttgacggctt
    15661 cctttgatag gactcaatat tcttctttat ggcttccatg agatcgtctt caggaatggc
    15721 agccattccg tcagccaaga ttgtctgagg gctttcgaaa agcccgattc gaggccatgc
    15781 aagcgcctgc atcctggtgg gcccctgccc tttcttaaca cagtgcaatt taaagagcat
    15841 tctcacatag cggcggtcat caacataatg ctccgcgcat gtccatcgaa cacaggtatt
    15901 ggcaatctcc ggctcatatc ctctttttag aagcttcagg tataattgtc ttgaagcctg
    15961 ttcgttatga gaaacaatta aaagagcatc agagcgagcc ctatgcagat cattaagacg
    16021 accgaggagc aataacgagt cttcatcgat tgattcatct tggaatagca aaatggattt
    16081 tggtccataa tgtccagcaa ttgcggattc tgagaattcg attgaatccg cggcaattaa
    16141 gtgcactgct gatgaatgtc ttgccaatgc atccactgag tgttctgccg gagaatgaac
    16201 atctaagaaa tatttgcccg aaaaaggcaa atccgacgga tgggtcttca atgaataatc
    16261 cagaagctgt gcatctactt catcaaaaca aaacacaaag cccccggaaa ccgccacttc
    16321 gagcgatccg gaggcctttt tgcgaacgga atcgatccgc ttcgccatag gtcgtcagcg
    16381 tttagaaaac tggaatcttc ggcgggcgcc gcgctggccg tatttttttc tttccaccat
    16441 ccgcggatca cgagtcaata ggccattggc cttgagcgaa atgtggtttg ccggatcgat
    16501 ctgagctaag gcgcgggcaa gcccatgaga acatgcgcct gcctgtccgg atagaccacc
    16561 acctactacc gtaatatatg cgtcgaattt attttcgcta tcggttacaa ggaacggttt
    16621 tctgacctgg gcaatcagac catcaaccgc aaaatagtcc tttagatcct tctcgttaat
    16681 gaatattttg cctgaacctt ctcgcagaaa gacccttgcg actgcgcatt ttctgcgacc
    16741 ggtaccaata ccaagattga tattctttac cattttcttt ccttttccgc tcaaatatct
    16801 attgcgatag gattctgtgc taggtgcgga tgatcgggac cggcatagac ctttgcattc
    16861 ttgaacagct ttcggccaag aggccccttt ggcaacatcc ctttgattgc aatctccatt
    16921 ggagataccg gattgcgggc aataagttgg tcaaaactaa aatccttaat accaccagga
    16981 tacccggtat ggtgatagta tgttttattc tggcgtttgc gtcctgttat ggcgactttg
    17041 tcagcattga taataatgac ataatcgcct gtctcttgac ttggagtaaa tgtcggcttg
    17101 tttttgcctc tcaaaatcga agcaacttta gctgctacgc ggccaagtgg cttgcccgct
    17161 gcatcgatta cataccactt gcgctcggcg gttgcagcgg taaaaaatgc ggtcttcatt
    17221 tatatacgcc ttcaccttat tgagaaatat caaaaaatgc gggtgcatat tcccacagtt
    17281 tcaaaaatct gtcaagcatc caagaccaaa agcagctacc agtctccttt gttctcggtt
    17341 gccttctctt agttttcgga attctcagac ttgttcttct cttctgcttt ttctttttct
    17401 tcttcttttt tctcttctat tttatcctta atatccgcga tcccaataaa aaccgcgagg
    17461 gcgccgatca ggaaagctat tacaggcaga gcaccccgaa gaaaagcaag aacatcgctc
    17521 caccagccca aaattcctgg aattaccatg agcacagcta atactattag tccaattcca
    17581 actaacaatg ctgtcatttc tattctcctt aattccgtaa attctcccac attcatgttt
    17641 agaggatggg catgtcacca attgttctca aatataacag aagcgctcag cattcgcaat
    17701 agatcattga tacacacttg tatatccaca ttatcgagat tataaacaac acatgatatc
    17761 ccctcattct ttggtgatgt ataaaacaag acctgccatt gttctttcaa acgatttaga
    17821 taggattttg atcaagacaa tagatggaga tgagcgcaag gtacgcaata aagacctggc
    17881 catggtccat gctggcccga ttcactcctt tccaattgct caacatagca aagaagtaaa
    17941 tgaagccctg gatctgctta aagaagaaaa cccggaaggt gttccaatag tctcctggaa
    18001 tgagtttgcc gaacttgcat ggagcaaagt cgaggccaag catatcgtta tagcttggca
    18061 tatgctgctg gaaaaccctg ccatagaaat gctcgataat ggctttagaa ttcgaagcaa
    18121 agaagaacaa cagaaattaa ttgaaaagga aaggaaaaaa aaggaaatca ccgaaaccag
    18181 agcagctttc gttgaggcgt tcgggcttgc gtggaaaaag aacgatccat cctttgttga
    18241 taaagaccag aatttcaagc cttttgtcga tgaattgagt aggtttgcac gcggcttcgc
    18301 agttgatagc ccaatagcgc gcgatcttgg aatcagattg actccacaag cggtccatga
    18361 agcattgctt gcaaccggat tttgggaacc ctttattaat ccttggccag atcgaaatgg
    18421 atgcatatta tcaatgcctt ccgaaccaaa gaatatccga gaacgatctg aattgacctt
    18481 ggaccgattg gacttgagat atctttcttc ctttgccatc gataatccat ggtccaaaga
    18541 tcccgacgat gccatctcta tagaaggcaa tagagtctgg atacatgttt cggatcctgt
    18601 ttccgcgatc gctcctattt ccaccgagga cgaggatgca tgcgcaagag cttccacttt
    18661 atatttaccg gaaaaaacaa tacccatgct tcaccactcc ttgatcgagg cctttggact
    18721 gggcatttta ccagaatcca aagctctctc attcggaatc accttgcaga aaaatggcaa
    18781 aattgatcaa atatctatcg taccaagtat tataaaggta gcaaggctta cctacgaaga
    18841 ggctgatgtc ttgctagaaa ctaatgaagt actacaaaac ttaaatgaaa ttgtggaagc
    18901 aagaaaaaag ctcaggcaag atcagggctc cgtggatatt gtattccctg aagtgtcgat
    18961 tagagccata gacggcaatg tcgagttttt gcctgtacct gcaactagat cggcaaggct
    19021 tgtacaagaa ctcatgattt tggcagggga ggcagcagca cgatgggctt atgcggctgg
    19081 ccttgctttt ccgtatgttt cgcaggagcc tcccatctcc gctgaatctg ctggatgcgg
    19141 agcgggactt tcggaaagcc ttgctgaaaa ctatgcgcga cgcaaggtta tgcggacggc
    19201 tattgtaagc tcaacctgcg gcgctcatgc aggccttggt ctatcatttt actctcaagt
    19261 gactagccca cttcgaagat atcaagatct gttagcgcat tatcagatac acgcagagct
    19321 tgcccgacag aaatccagca atatattaga ggaaggaatt cctagagaag cgcaaatagg
    19381 actcagccga gaaatgcttg accaaaggct ctacctctat ggtctgcaag cggcaaagaa
    19441 tcgccaagct gaaagagatt cccgccttca ttgggcaatg gtttacctca gcaaacatag
    19501 agattggaaa ggtcaagcga ttgtcctaga ggtcgacaga ggccatgctc atatctatat
    19561 accatctttc ggaatcgaaa tgcttattca tatatctcgt aatgtaaaac cagatacgaa
    19621 cataactatt gcgcttcgac gggtttcgat tcctgagctt accgcaactt tcgatattgt
    19681 ttaggattag ctaaattcat caaaaaatta ttattttatt gcacctgcaa ccattccttt
    19741 gactatgtat ttttgagcaa aaaggaagaa tacgattacg ggaatcattg ccataagtat
    19801 agctgtaaga atgagatccc attgtttgac aaaggctcca gcaaaattgg atacagctaa
    19861 aggcacagtc attacatcat tgcctttgcc aagcacaaga agaggcagaa gataatcatt
    19921 ccaaatccat ataccattaa gaaccagcac ggtagcctga attggcttga gaattgggaa
    19981 aatgatccta aaaaagattt ggtgccgatg acatccatcg atagtggccg cctcctctaa
    20041 ctccaaagga atacttttga tgaaaccatg aaacataaat atagaaagag gtgcaccaaa
    20101 gccgacataa cttagaataa tcccctgata agtgcgcagc aaccttatgc ctgtagcaac
    20161 ggtaacggtg cgaaaccatg ataaaagagg aaacatgaca atctggaaag gaatcaccat
    20221 cgaagccaca aaaatgaaga atatgaatga agagacctta gtttttgtcc tcactagagc
    20281 ccatccagcc atcgcagaaa agacattgat caaaatcaga gaaacggccg taattatggc
    20341 acttgaaagg agggagctag gataacaaac cgactcactg gtccaaattt tgatgatatt
    20401 atcgacaatc gtctcccatt ttttaggcca ggccagtgga tactgtgtca cttcgaaaga
    20461 tgtcttagcc gaattgatga gtacgaggaa aaaggggaat aagaaaagca caaaaagaat
    20521 gatggtgagt atttcggcaa ggaaagatga tggtcttcta atcttactgc ttttcataac
    20581 tcgatctcct ttttcttgtt cacgttcacc tgaatgatcg aaatgatcgc tagtatcaag
    20641 aaaaagataa acgcccttgc ctgtgcttcg gacagcttgt tcgagataaa agctgtattg
    20701 tatatgttga gagcaagaag ctccgttcca aaaataggtt gacccatcaa catagtagaa
    20761 ggtccaccgc tcgtcagcga aacattgaca tcgaactgtt tgaatgaatt gacaagcgta
    20821 agaaacattg tgactgtaaa agcctgagca cacagaggaa ttgttataga tttgagacgg
    20881 atccatggag tagccccatc cattcttgaa gcctcgatga gttcctgggg aatattttct
    20941 atcgctgcaa catagatcat cattatgtaa ccagcgtatt gccatgtccc cactatgatc
    21001 attgcggcga tagcagtgtt gggcttgctt agcattagat tggctggatc agataagaaa
    21061 tcaaaggcag atatgataga accaagcgaa ggaagagcat tattaaagat aaactgccaa
    21121 atatatccca gaatgatccc tccgatgaga ttgggcagga aaaagccgac tcgatataca
    21181 ttcttgaact taagatcgcc cgttaccaac agagccagca caaaggccac aatatttatg
    21241 acgatcatgt ttaaaatcgt atataaaaag gtaattccga aagagtataa aaatgatgga
    21301 tcttttaggc tattgataaa attctcgaaa cctacaaacc tcaagacgct atctaccctt
    21361 gccgatgaag tccaattagt aaacgcataa taggagccca atataaatgg aatcaccatt
    21421 accatgataa acgcaactag cacaggcaaa agaaataccc aaaataccaa tgcagattcc
    21481 attttttttg tcatgaatgc tccccttctt tatcgaggat tgaaaaacaa aaccgcctgc
    21541 atccacatta gcaatgcagg cggaccaaat caagcaattg cgcggacagc ttcgctggaa
    21601 aagtattcta ctatgataga ttacttcttg gcattatcct tgaatgcttt ggtcatcaga
    21661 tcgatgaatt gagccttgct gatgctgccg gctgcgaact ggttatagat cggagtaagc
    21721 accttgtctc taaaatcgcc ggagaaataa tattgattcc agctatagac cttgccggct
    21781 gccgaccact gctgaacaga tttggatagc tgaccttctg gcccgagagt aatattcgag
    21841 aaggctggaa tcatgcctgc ttccttgacc atgtagttat ttccttccgg agttacgaca
    21901 agatcgttaa gaaatttctt agcaagcgct atgttcttag attccttgtt gattgcataa
    21961 tacgatggag ctgatacgaa gataccatcg gtaactttct tcatcgatcc atgaggagca
    22021 aaagccatct tgaatgtggc atttgcatcc tttaggttag gatctaccca gttcccctgg
    22081 tgcaagaaga cagccttgcc ggtggcaaag gctccaactt gagcgtcata gttgccggta
    22141 agaagaattg ctttatctga atatttgaat agaagctcta cccagtctgc atactcctga
    22201 agcctctttg catcgacctt gccagccaac agatcgttcg taacagatag gtcgccataa
    22261 ggcaagccat tggatagcaa ggagttgaaa ttatgatgag cggtaaccca tcccatctca
    22321 atggaagcgg ccatagatac aaccgaattg atacccagat ccttcttcat agaatctagc
    22381 ttttcgaagg ccttcttata tgcatcgaag ttgttcaaag tcttgggatc tataccagcc
    22441 ttagccaaaa ggtcagaatt gtaggccaat ccccatcctt cgattgccac aggaaatccg
    22501 tagacctttc cattatactt gaagggaaca gaggtcttgg aaacccaggc ttcatttgag
    22561 aggtctagta taattccttc ccactcctta taagcctcaa ggccatcgaa ggcgaagata
    22621 tccggcatat ctcctgccgc atagtcagct ttgagttgag gacccatgcc gcccgaggtt
    22681 ccaccaatag atttgatcac aacagttgcg ccatttttct ggccccatgc ggctgcatag
    22741 gctttaagct gagcatcgat ctcgggttta ttctgcatca aggtaagttt accagcgcca
    22801 aatgcagcag taactgtcaa cagcgccatg aatgccaaca aaaacctttt catactacat
    22861 cctccttcat gctattcgat taagttgggt tgcttaaccg gtttaataat agtagcataa
    22921 aaaactatat tgtcaagact cgattaaaat gtccccatat atagttcgat taaaatttcc
    22981 cccttcactc aggctgagcg agaggctagc tccttcttgt atggatgaga cagtggtgca
    23041 ggcgctgcat aagagaaatt caactgtgca atcgaagcac ttcatgtgcg tgacacaagg
    23101 attttttgat ggccatgagc cctatcatta cctgtgccac ctattcaacg agctaccaaa
    23161 ggcaaagagt cttgagtaga agcttgcatt cttgccatac tgactggcac cgactcatta
    23221 ctaattttaa gcaacggtgt ctatttgacg cttaggtaga tagtcccaag agctctgcat
    23281 tcacattgct tctcatctca tcgcgcaggt tcgagcgtgt aggaacgctc atacgagcca
    23341 taagcctttg tggtgatgcc gtttcgtgtt gagccttcat agttaggctg tccttcaagg
    23401 acatggacaa tgacgggtgc gctctctcct ggtttaagga ttcctgcatg cactgctgcg
    23461 ttcgcaatat aagaatctga agtgtagatg tcggtgcccc aaaccttgcg ctggtctgcc
    23521 atgccggtaa cccaaacaac atagctctta cccaccctca cattggggat atagtctgcc
    23581 gtaccggggt ctagaatcat ccacacaact tggtcgtagg attgcgtcac aggctcgagc
    23641 gaataactca ttccatagtt tccataattc atcgatgtga tattatggcg cgtactaccg
    23701 acgtagctct gttggccagg gagtatcgta actttaacaa tgccagtctg cccgtccgaa
    23761 agaataccag catgaacagc cgcagcgcaa agatcggaat ctagggtata gatatcggtt
    23821 ccccagacat ccgctcccga gacttttcca gttacttgta catagaaagt agtacctggt
    23881 ttaaagttcc catttctttt gagcacatag ggatcatcaa aggcgtagag attgcgactc
    23941 gctactgggg gctgtggcaa tggtggagtg gatggcgtgg gtggcttgag ctgagtaggc
    24001 ggtgtttgtg ttgctggtgg cgcgctggga gtcgcttgcg cgaggcttcc agaggcttga
    24061 gcaagcccaa cgaacctgaa ggacaggcta taacttcccc atgagttgct ctgtacgcca
    24121 tttctaaggg agccagaata tgaagaaagc ccatcacaaa taacgactgc cacaatccct
    24181 gtttgaccgt ttgctataac ccctgcatgc accgcagcag cggcaacatt ggaatcgagg
    24241 gtatagatgt cggttcccca aagcgataag gctccactca catcgccctt aatcgataga
    24301 aataacaccg atccaggctg aaggcctgca atagtgcgaa gattcggtga agactggcca
    24361 aataccggta caagagcaaa aacgccaata atgcaaataa aaagaacaaa caattgtcta
    24421 aggttttcct ttctttgcat aatatcgcct cctttcagac tcctttcaga gttgcttcgg
    24481 cacctaccga agaacaaaat gtgcatatca atggtgcgaa aacaatttaa tcatctgttt
    24541 taacctacac cgtctttccc aaaaaagcaa atcaatcgga gcgaagattt aaaaatcaat
    24601 ttaaaaaaat caatggttcg tgacactatt aatttattcc tttcgcgaaa agggaatatc
    24661 tgaaagtact tgctctagca taccacgctt gatctcccct ctgatcgttt tggcgctctt
    24721 cctaagagat tttgcaaagc atgattagac ttcgcagctt ctgatagcca ttactgcttg
    24781 cgctaacaca ttttcctttt aggtgggcat tttatgttac tgctcac
//
LOCUS       DAOMZL010000021        23591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_1823_length_23591_cov_16.893270, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000021 DAOMZL010000000
VERSION     DAOMZL010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 23591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23591
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1823_length_23591_cov_16.893270"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /gene="dnaA"
                     /locus_tag="PLQ30_03105"
     CDS             complement(<1..980)
                     /gene="dnaA"
                     /locus_tag="PLQ30_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPW01463.1"
                     /translation="MSDDNGTIWQKALEIAKSQVPESEFIMWFRLSYIGYENGTILLQ
                     ANNTFLRDQFVRKYSQFMREIISSLLGMPVELSVEAQSKTIDHHIAPQVSPRKAKNNI
                     GAEKVIEAQPGISRSARPSNLQSRYTFESFVVGENNNFAFNAAIAIADKPGSSYNPLL
                     IYGGVGLGKTHLMHAIGNRIVERHADFRIICVTAEEFTNEFIKTIHDRTTNDFKNKYR
                     NVDVLLIDDIHFFQSKHGVQEELFHTFNALYDTNKQLVFTCDRPAAELKDFSERLKSR
                     FMMGLKTDLTTPSFETRAAIVRKKLELYKRHFGEDVINLIAKSIETNVRDL"
     gene            1407..3323
                     /gene="gyrB"
                     /locus_tag="PLQ30_03110"
     CDS             1407..3323
                     /gene="gyrB"
                     /locus_tag="PLQ30_03110"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPW01464.1"
                     /translation="MESKTSSYSANTIQVLKGLEAVRKRPGMYIGTTGSEGLHHLVYE
                     VVDNSIDEALAGFCKKIVVALERQDIIRVEDDGRGIPVDIHPTEGISALELVMTRLHA
                     GGKFDKKNYKVSGGLHGVGVSVVNALSAWLEVYVHNDGKVFYQRYVRGIAEKPVAFIG
                     DTDRTGTIVRFQADPDIFEETVYSFDVLSNRLRELAFLNKGIKISLIDERLSQPKRIE
                     FHFEGGLQEFVEYLNKNKTVIHKDVIYFLGTRDEVEIEIGMQYNDGYNETMFTYVNGI
                     NTKEGGTHLIGFRNALTKVVNDFFKKSKYSKKIEETLSGDDVREGLTAVLSIKVLEPQ
                     FEGQTKSKLGNTEVKGIVEGFFSEQLDLYFQKNPEVIDIILGKCATAARARIAARQAR
                     ELTRRKSLLEASSLPGKLADCQEKDPSKCELFIVEGDSAGGSAKMGRDRLFQAILPLW
                     GKMLNVEKARLDKVIGNDKLQPIIASLGTGIGEDFDISKLRYHKVIIMADADVDGSHI
                     RTLLLTFFYRYMTELIERGHVYLAMPPLYRINYDKEIHYAYSDEDKDKLLLQSNKDPS
                     KISVQRYKGLGEMNPEQLWETTMDPSRRIIKRITMEDAILAEEMFVTLMGEQVEPRRR
                     FIEENALEVSNLDV"
     gene            3352..5781
                     /gene="gyrA"
                     /locus_tag="PLQ30_03115"
     CDS             3352..5781
                     /gene="gyrA"
                     /locus_tag="PLQ30_03115"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015713162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HPW01465.1"
                     /translation="MAESSNIIPVPIEEEVKSSYLNYAMSVIVSRALPDVRDGLKPVH
                     RRLLYAMDELGLRSNATTKKSARITGDAMGKYHPHGDQSLYDALVRMAQPFSLRYPLV
                     QGQGNFGSIDGDPPAASRYTEAKLSKIGEEMLADLDKETVDFVPNYDESLKEPSVLPS
                     AIPNLLINGSSGIAVGMATNMPPHNLREIALAIEAFIDDPDITIDVLMKNIKGPDFPT
                     GGIIYGLQGIKDAYETGRGRLTVRGRFIIETMKSGREQIVFTEIPYALNKTMLVTRIA
                     ELVREKQIDGISDLRDESDREGIRIVLELRKGAITKIVLNQLFMRTPLQSTFGVINLA
                     LVDGVPKCLNLKELIYYFVQHRFEVVTRRSKFELKKAEERAHILRGLVIALGNIDEVV
                     AIIKSSKNIDTAKTRLSERFELTEAQAQAIVDMRLGRLTSLETEKILAELKEIEARIE
                     YLKGLLSDPNSIRQVIKQEIHDLAEKYGDDRRTEIVPNQVEQINIEDLIKPEEMVILI
                     SNKGFIKRISVNQYKSQGRGGKGSNSAALIEEDFVEQLFIANTHDYILFISSWGKAYW
                     LKVLEIPETSKQARGPHIRSLLAISEDEEISAIVDFSDFSEKEYILMGTLKGVVKKVA
                     TSEFANAKTRGIIGITLDKGDRLISAVLTQGSDEVVLISRRGQALRIHESQVRKMGRT
                     ARGVRGISLHESDELAAMLRVEPNESMLIITQNGFGKRVSYQLFNVHSRGTKGQTIYS
                     PDEFSGEVVKGVSVQEEDEVMVITSTGKTIKLSVSSVRQMGKGARGVRIVNIEPPDFV
                     IGIDKIVQEQE"
     gene            complement(5942..7039)
                     /locus_tag="PLQ30_03120"
     CDS             complement(5942..7039)
                     /locus_tag="PLQ30_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPW01466.1"
                     /translation="MAEIFLKNEKLFVNLPELGQWLSNEMQDFACSGKMLRGMLAVVG
                     SRLLHDERILVADYEDHKAISAAAGLELLHAGLLVHDDIMDHDHKRRGKPTFHLRIKD
                     ILEKIHANSAAAHAFSIAEAQGICVGDLFFFLGWQEISGLDLSGLDLDISSLFARELA
                     LVTIAQIRDVELGYDKSIPDIDEVIEVYRHKTARYTIVLPLVAGVLVAQSSLARASAS
                     QTAQIMDKSIIPLLEDFGESIGIVFQLQDDRIGLFGNEKETGKPVGSDLKEGKKTPYI
                     ILLANRLEKGERERFDSIFASPTQDLKDIEWIRNTVMAKGVEADIQKMIDSYLSKAQA
                     DLNELSALPSIESKTISLLEGLIQYSGSRSS"
     gene            complement(7197..8075)
                     /locus_tag="PLQ30_03125"
     CDS             complement(7197..8075)
                     /locus_tag="PLQ30_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPW01467.1"
                     /translation="MVQNKKLKETRSSAHYDDENVLSMYLREINRIPLLSREEENEYA
                     TRAAAGDEKAKEILAKSNLRFVVNVAKKYQNQGLPLADLIAEGNIGLLSAIDHYDVTK
                     GYHFISYAVWWIRQAILKAICEKSRMIRLPLNRANELVQIEKARSLFEGEISEEDEIR
                     EVARFLNMEPEHIKDIISISRDLISLDAPVYDERNSTTVGELIENEKHESPEQNAIQS
                     SLRDDINEVLSTLTAKEREIVEYRFGLNGKRPMSLKEIGDRMHLTKERIRQIEKAALK
                     KVAVPETMEMLQDYVA"
     gene            complement(8290..8958)
                     /locus_tag="PLQ30_03130"
     CDS             complement(8290..8958)
                     /locus_tag="PLQ30_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HPW01468.1"
                     /translation="MRTSSDNEKANSYLRRGLHELSRHKPAKALGLFRKSIELTPPSC
                     EKKLSRAFYWLSIALLQLNKRDLAVRSLANAQRMNRKGYIRRFYVRHVNGYGMIKQPT
                     KELDDLYAFLSIQLSFYLVKRPNYRFSSEAEHSIILSFLLNAWKSIKDSQEFESLDCS
                     EKLMLFNKLKIEFPAFAPDSMVQRKKERQFLQSSMAYIQPCSCGSGLPFMQCCGRTRG
                     ISEL"
     gene            complement(8983..11256)
                     /gene="glgX"
                     /locus_tag="PLQ30_03135"
     CDS             complement(8983..11256)
                     /gene="glgX"
                     /locus_tag="PLQ30_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HPW01469.1"
                     /translation="MNDALKSLPRKLTLMPGNPGLLGAIPKAEGVYFSIFSRNARGMI
                     LCLYEKPHDDIPAASLQLDPMRNRTGDVWHCFIPGLESEVLYLWRAEGPFAPHEGHRF
                     NPYKVLIDPYAKAITDGSLELCSALAYDEESPDKDLSLSTIPNDGSMPKCVVVDDEYN
                     WENDTPLNYPLKNCIIYETHVRGLTKSLTAKVKHHGTYRGVVEMIPYFKSLGITSLEL
                     LPVQEFDSSERFRRNPITGERLSNYWGYSPIAFFAPESSYAFDEGIDHDSYCPSHPVK
                     EFKYMVKELHKAGLEVILDVVFNHTAEGNELGPTLSFRGLDNSIYYMLAEDKRYYRNY
                     SGCGNTLNCNHPVLRTFIMECLHYWVVTMHVDGFRFDLGSILGRDSQGNLLANPPVIE
                     SIAEDPILRDTKIIAEAWDAGGAYQVGSFPGGRWAEWNDRYRDDVRRFWRGDEGFAPL
                     LATRITGSSDLYLNNGRRPFHSINFVTSHDGFTMNDLVSYNYKHNEANGENNRDGIAE
                     NFSFNYGTEGPSNAPFIEETRNRQIKNFFATLLLSIGTPMILGGDEFRRTQNGNNNAY
                     CHDSELNWYDWSLLKKHKDIYRFVKSLIALRKNHDAFRREDFFRGVGKRSIGTFSADG
                     QSRQNKAKSDRLSSTETPPDILWYDENGQAPKWNKIGKVLVARINEHFSSSELPCSYL
                     LAFNASTEAIQVCLPHDNEHDIWKRVIDTSLPPPHEIEEKEQPVLLDQQKYLMAPRSF
                     ILLISICEPVSDRNLPL"
     gene            complement(11253..12491)
                     /gene="fabF"
                     /locus_tag="PLQ30_03140"
     CDS             complement(11253..12491)
                     /gene="fabF"
                     /locus_tag="PLQ30_03140"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPW01470.1"
                     /translation="MAKRKVVITGLGLVSPFGNDVPTFWQNVKANRSGVGPITLIDSS
                     DLAVKIAGEVKNFDPSKKMDPKDAKKMDRFAQFAVYAALEALESSNIPREYLDPIKTG
                     VCLGTGQGGTNTIEESIMRLAEKGPNRVPPMTVAKGLANFGAAQIALSLGVHGPNMTV
                     VTACAAATDAIGQAMHLIRDGHADIMFTGGSEASIARMCMASFINIQALSVRNDEPEK
                     ASRPFDRDRDGFVMSEGAGILVLEEYEHAKARGAKIYAEIAGFGSTCDAHHLTAPDPE
                     GLWVSKAIEIALNDAQMKPEDIDYISAHGTSTPLNDPIETKAIKNAFGAHAYKLKISS
                     LKSMIGHCIGAAGALETIAAVLGMNENYVHSTINLDNPDPECDLDYVPHKGINMPVRA
                     FIKNSMGFGGQNAVLVITKP"
     gene            complement(12494..13204)
                     /gene="fabG"
                     /locus_tag="PLQ30_03145"
     CDS             complement(12494..13204)
                     /gene="fabG"
                     /locus_tag="PLQ30_03145"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPW01471.1"
                     /translation="MEGTLSGQVCLVTGASRGIGLAIATRFKQEGAIVYGLSRTKAEA
                     DIDWISCDVTDELSVESAIDQIFKIENRIDVLVNNAGMTKDGLIMRMKTEDWEAVLNA
                     NLRSAFFLSRAVSRIMLKQRSGCILNMSSVVGLHGNGGQANYAASKAGLIGFTKSLAK
                     ELGSRNIRVNAIAPGYVETSMTQVLPETAKENLKSSIPLGRPGVPEDIAEAALFLCSS
                     HAAYITGVVLNVDGGMGM"
     gene            complement(13274..14194)
                     /gene="fabD"
                     /locus_tag="PLQ30_03150"
     CDS             complement(13274..14194)
                     /gene="fabD"
                     /locus_tag="PLQ30_03150"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPW01472.1"
                     /translation="MKNCFLFPGQGAQYPGMAKDFYDASKAVRELFNEASEAAAMDLK
                     KLIFEGDEETLRQTRNTQISMVVAEAASALMAREHGIMPGGAAGFSVGEWPALAVAKV
                     ISSFDMFRLVSERGRLMEEAGNRAGSTTMSAVLYLSPEKIEQVIHEAGLRQCWIANYN
                     SPVQCVISGTEADIAVAEERLRQAGAKRVIRLKVSGAFHSPLMQDAYESFKELVEATQ
                     FSDPKINLYSNVTGKKMLSKEDAKKYASLQIISPVRWIDEEKSIAADGYDYCFETGPG
                     TVLAGLWKASGSSIPCIAAGTLQDLAAFSL"
     gene            complement(14326..15243)
                     /locus_tag="PLQ30_03155"
     CDS             complement(14326..15243)
                     /locus_tag="PLQ30_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01473.1"
                     /translation="MFQLTDAILERIIFAMEDQSKKFLIDLETGDLIDKTALENDDTS
                     KPYLEIGQRFVEPPSWDSHRGFSILEAFAATVTSPASLKIELNAALRRGKGVFRQFRQ
                     VLSKDQELFMRYQEFKLAAMRPIVERWLETLQEGEMLMALKEEPDDLRDIIASDIGFM
                     ISGPDEIAFELESYIDCCIEESFGSLPIAIKRRSLSDLFKEVRTDKNSLFVAFGTIDG
                     TSPLIAGLFLLQTYRGSLMAVVHGVFATKESAMLDLEWPLIECISDYCARIGATQILL
                     EGPFFSSLLCDNALEHGFFRSGTIFYRVL"
     gene            15312..16232
                     /locus_tag="PLQ30_03160"
     CDS             15312..16232
                     /locus_tag="PLQ30_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPW01474.1"
                     /translation="MLSNLHLHSRFSDGMSWPEDIALEAARAGLDIVALTDHDTMGGT
                     ERFIQACEARSLKAIQACEIDICEPQIEYKSELLAYFPKADLDSSILYTKRLLQKSLE
                     QRRRRLEFFIESAQELYPDKELSFQDLFRDKTGLDFDEELARKISWSKVDLFLYLKAH
                     RCIKPEMNYKEFKKRFFASGLLKKFKLDKPDVATVVKAVHGDGGFVVIPHLGHFWDDE
                     LPLFQKEAEKLRLLLAWFRRAGVDGVELYWYNSEFKSASFNEQLREIARPQGFFFTYG
                     SDCHGPGSGKHTIMKFKGPFDGFPQKKSIS"
     gene            16229..16801
                     /gene="pth"
                     /locus_tag="PLQ30_03165"
     CDS             16229..16801
                     /gene="pth"
                     /locus_tag="PLQ30_03165"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPW01475.1"
                     /translation="MIRLCAFLGNQGQEYRYHRHNVAWLFLDSMRIASELRWSSKFKG
                     AFTQIELGSTIIYFLKPHTFVNLSGESVGEAVHFYKLGLDEILIVHDELEMPFGAFGY
                     KFSGGLGGHNGLRSLEKHLGTRDFWRLRFGIGRPEHDDVAGYVLSPFNPEQLEVLNKK
                     VFPLAERTFYELFYEGIEGFDVRYKKVLPP"
     gene            complement(16859..17734)
                     /gene="era"
                     /locus_tag="PLQ30_03170"
     CDS             complement(16859..17734)
                     /gene="era"
                     /locus_tag="PLQ30_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPW01476.1"
                     /translation="MPKSAFVAIIGRPSVGKSTLLNALCGAKVSIVSSVPQTTRNAIR
                     GIITRPEGQLIFVDTPGYHISDKKLNLRLREIVLRSLSEVDLILYLIDATREPGKEEE
                     AIAELLSKDIHRIVVAINKIDDPEARPLLIEEFIIPRLRGAKRVSISAVEKTNLESLI
                     EVLFSLAPEGPFWYPEDIYTDQEPVFRIGELVREKAILNTYQELPHAIAVEYRESSKR
                     ADGVLFARFDILVERDSQKAIVIGRQGSIIKRIREEAEADLKELFDYPIKLQLQVRVD
                     PDWRKSDSALSRIIF"
     gene            complement(17806..19332)
                     /locus_tag="PLQ30_03175"
     CDS             complement(17806..19332)
                     /locus_tag="PLQ30_03175"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HPW01477.1"
                     /translation="MEKNDRPKICLSMTGKTIEEDLRVLEQYRSVVDCVELRADCLDP
                     SERFFIRDFPEKAGLPCILTIRRLKDGGKFEGGEGTRLVLIARALSFPKPDARANYAY
                     VDLEDDFRAPVVEESCRTFGTRIIRSHHCLGGMPDSLDAAYEELTSEEDEIPRLTVNP
                     QNSADFIRFLEWCLQKPRCDQILIASGDYGIASRILSWRLGSLWTYASPLRGGMDAAA
                     PGHIDPFDLVNIYNFDLVDNDTMIYTLMGQNSIAKSLSPYLHNSAFRKMGKNALLIPT
                     PVDNCEDGLKMLEILGGTGAAITVPFKQDILPYLSFHSTDVQLIGACNTLVLRESGWA
                     GYNTDADGFERSLLEFLGKQDISGCKATIIGAGGAAKSVALSLFRMGARGLVLNRTYS
                     IGRDLARKYNFLYGHLDDRAIDLLLEYSDLIVQATSVGMENEGDPISFYEFKGSEAVF
                     DLVYYPIKTLFLKRAEAAGCRIMNGFKMLCYQAAGQYKLWMGEPPPAIYSQLEQPEQI
                     "
     gene            19492..20184
                     /locus_tag="PLQ30_03180"
     CDS             19492..20184
                     /locus_tag="PLQ30_03180"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HPW01478.1"
                     /translation="MPDNSDAITSRFNEKALKAFLDGLLDELRHPQDPKLLEDVRRAF
                     RKKIPLHLRSYAAALMILRAAGLSRAKPQQKMPQQKASPQKSPTPKVPPQPQKKSARN
                     ANWQQGKSSFTAVPSTSASIRSTPSSTSSSDSPSSTQELPSKETVSLFVSMGKRHHLK
                     PQELRNEIAEHTGLSLDEIGRIHLLEKYSFIEVPEKEAQKVIAAMNGAELNGHVLEIK
                     PAKKRAKTSFRG"
     gene            20191..20883
                     /locus_tag="PLQ30_03185"
     CDS             20191..20883
                     /locus_tag="PLQ30_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW01479.1"
                     /translation="MASTIWRLKVGLIGENCYIVESEGKILLIDPGAEAEKIDDFMRY
                     KGIKPSLIILTHGHLDHTAAIPDLFEIWGTELPIAIHPQDAAYLGKDSEQTNRLLFEA
                     IKAPSYFHASWKTMPPPGIFLTDGMAVPGTSLQVLHTPGHSRGSICLYEPELEAGSYG
                     YEEKSMVQGANPSCIISGDTLFRDGVGRTDAPDSDAYALEASLRRLARFSPDTLVFPG
                     HGPRTTIGRELS"
     gene            20997..22040
                     /locus_tag="PLQ30_03190"
     CDS             20997..22040
                     /locus_tag="PLQ30_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPW01480.1"
                     /translation="MESSKFISSHFLVISFVLACTIASRPSFLYAKELSKTDFALGTL
                     CTIRLVEGGNSATLDQAFLRLRTIEDRMSVNKEGTEVSKINEMAGKASVEVSSDTFYV
                     ISKAIDYARLTGGAFDPSIGPLTKLWNIGAGGEKVPPKENILSAKALVDWRNVVLDAS
                     ANKVFLKEEGMSLDLGAIAKGYAADEIEKILRLAKVKAAVIDLGGNIFVYGTKKDKTL
                     WRVGIQNPESERGDYLGIVEGPQMTIVTSGIYERCFIENGKRYHHILDTKTGFPVDNG
                     LVSVSIIGQSSIDADALSTSLIALGIEKGLELLKRFPNTYAIFIDKDKMVYLSPGAEK
                     VFTLLDKNYTLVR"
     gene            complement(22022..22993)
                     /locus_tag="PLQ30_03195"
     CDS             complement(22022..22993)
                     /locus_tag="PLQ30_03195"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPW01481.1"
                     /translation="MGIPDAFLSFIFSLAAIIVIVMMSVESMHAAEAQIILQIQTFLG
                     LGLRIPMTIVSLFASEAFVIAIVPIVYWCVNRKKGAELGLLVLVSTFINLWLKQIFGW
                     PRPFDIIPSVGLASETSYGMPSGHSQLSVVFAAYIIQFLPKKWRPLMLIGFPLLIGFS
                     RIYLGVHFLTDVLGGYLAGSLFFILYKLGGPSIEKILQKADVRGRVILAAALSFVMNL
                     ILPKETMISGAFFGAAIGFTFAARSLAFNEKDRLKIKISRYLVGLITTGIVYVLLKSA
                     SGPINNLLGNNQENLLRFLRYALVGGWVSFGVPFLFLKVGLAHRTSV"
     gene            complement(23056..>23591)
                     /locus_tag="PLQ30_03200"
     CDS             complement(23056..>23591)
                     /locus_tag="PLQ30_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01482.1"
                     /translation="LEIGDGRAFVEQCPDNSFDVVIMDMTDPFGPSERLYTKEFFMQV
                     QRILKGPEGIFAMHGESSIARPIGWACIYRTLKDVFHIVRTAFAFVPMYGTLWSFQFS
                     SDAIDLLTFSNPIIKSMDELEARIAEKRSEEPRFAKAAMWPSLFAKDPTAKAALSFPQ
                     SCVITDSEPHFPDAFEP"
BASE COUNT         6541 a         5320 c         5425 g         6305 t
ORIGIN      
        1 agatcgcgca cattggtttc tatgctcttt gctatgagat tgatgacatc ttcgccaaaa
       61 tgtcgtttat agagttcgag cttttttctt acaatagcgg ctcgcgtttc gaaactgggg
      121 gtagttaggt cggtctttag tcccatcatg aatctgctct ttaggcgttc agaaaagtcc
      181 tttaattctg cggctgggcg atcgcacgtg aaaaccagct gcttattcgt atcatagagg
      241 gcattgaagg tatgaaagag ttcttcttgg actccgtgtt tgctttggaa gaagtggata
      301 tcatcgatta gcaagacatc gacattgcga tacttattct tgaaatcgtt ggttgtgcga
      361 tcatgtatgg tctttataaa ctcgttggtg aactcctcgg ctgtaacgca aatgatccgg
      421 aaatcggcat gtctttcgac gatccggttt ccaatggcat gcatcaggtg tgttttacca
      481 aggccaactc ccccataaat gagaagaggg ttgtaagaag accctggctt atctgcaatt
      541 gcgattgctg cattgaaagc gaaattgttg ttctctccaa caacgaaact ctcaaaggta
      601 tatcgagact gaagattgct ggggcgagcg ctccgagaga ttccaggctg tgcctcaatt
      661 actttttctg cacctatatt gttttttgct ttccgagggg agacctgagg ggcaatgtga
      721 tgatcgatag ttttagactg cgcctctacg gacaattcta caggcattcc cagaagggat
      781 gaaataatct ccctcatgaa ttggctgtat tttcggacaa attgatctct aaggaaggta
      841 ttgtttgctt gaagcaggat ggttccattt tcgtatccga tatatgatag tctaaaccac
      901 atgatgaatt cggactccgg aacctgcgac ttggcgattt caagagcttt ttgccagatt
      961 gtaccattat catcgctcat gtgtcgaaat ctccaagaca tcattgcaca tttccacaag
     1021 ttttccacaa gaagccatag attttttgta taccaaagca ccggagagtg caagataccg
     1081 agagggcaaa gttcgatatt ttttgactat ttgtttagga aacacgggaa atgcggccta
     1141 ttatagggct atttatttat tgaatttttt aggaaccggt tattttgaag gattctcttg
     1201 taagctaaag aaattatatc acaaatacac aggctaacca caaagagata acaagttatg
     1261 cacaggtttt ccacaaatgc agaagagcta gcttttgacg accttttgct gttgagcttt
     1321 actcaattct ctctatttgg tatgataaga aatgagattt atcgcaaatc ttacccctat
     1381 tttctgcaat tcaattcagg ataaccatgg aatctaagac tagcagttat tccgcaaata
     1441 ccattcaagt gctcaaaggg ttggaagcgg tccgcaagag gccgggtatg tatataggta
     1501 ctacgggctc tgaaggtctc catcatctgg tatatgaggt cgtcgataat tcgattgacg
     1561 aagccctggc aggcttctgc aagaagatcg tcgtggcgct agagagacag gatattatcc
     1621 gggtagaaga tgatggccgg ggcattccgg tagatattca tcctaccgaa ggcatttccg
     1681 ccctcgagct tgttatgact cgtctacatg ctggcggaaa attcgataag aagaattaca
     1741 aggtttcagg tgggcttcat ggcgtaggag tctctgtggt caatgcactg tcagcctggc
     1801 tcgaggtata cgtgcacaac gatggaaagg tattctatca gagatatgtg cgcggcatag
     1861 cggaaaaacc ggttgcattt attggcgata cagacagaac aggcacaata gtgcggtttc
     1921 aggctgatcc ggatatattc gaagagacag tctactcatt tgatgtgcta agcaatcgct
     1981 tacgagaact tgccttcctg aataagggca taaagatatc tcttatagat gaaaggcttt
     2041 ctcagcccaa acgaatcgaa ttccacttcg aaggaggcct tcaagagttc gtagaatatc
     2101 tcaataaaaa caagaccgta atacataaag atgtgattta tttcctgggt actcgcgatg
     2161 aagtcgagat agaaattggc atgcagtata acgatggcta caacgagacg atgttcactt
     2221 atgtcaacgg cataaacaca aaggaaggcg gtacccatct tattggcttt aggaacgctt
     2281 taacaaaagt agtcaatgat ttctttaaga agtctaagta tagtaagaaa atagaggaaa
     2341 ctctttctgg cgatgatgtg cgagaaggcc ttaccgctgt tctatcgata aaagtgttag
     2401 agccccaatt cgaaggccaa accaaaagta agctaggcaa taccgaagtc aagggaattg
     2461 tggaaggctt tttctctgaa cagctcgatc tttactttca gaagaatcca gaggtcatag
     2521 acattatcct tggaaagtgc gctacagcag caagggctcg catagcagcg agacaagccc
     2581 gggaactaac caggcggaaa agccttttag aagcatccag cctgccaggc aaactcgcgg
     2641 attgtcagga aaaggatcca tcgaaatgcg aattattcat tgtagagggc gattctgccg
     2701 gtggatctgc aaagatgggc agggacaggt tatttcaagc cattctgcct ctatggggca
     2761 agatgcttaa tgtggaaaaa gcccgcctgg acaaggttat tggcaacgac aaactacaac
     2821 caataattgc gagtcttggc actggcatag gagaagattt cgatatttcg aaacttcgtt
     2881 accataaagt gataataatg gcagatgcag atgtagacgg ctctcatata aggaccttgc
     2941 ttctcacttt cttctatcgc tacatgacag agcttataga gaggggccat gtctaccttg
     3001 caatgcctcc tttataccgc ataaattacg acaaggaaat acattatgct tatagcgatg
     3061 aggacaagga taagctttta ttgcagtcga ataaagaccc ttcaaaaatt tcggtgcagc
     3121 gatataaggg cttgggtgaa atgaatcccg aacagctttg ggagaccacc atggacccca
     3181 gcagaagaat catcaagcgg attaccatgg aagatgcaat ccttgcagaa gagatgtttg
     3241 taacccttat gggcgaacaa gtggaaccaa ggcgccgttt catcgaggaa aacgcgctcg
     3301 aggtatcgaa tctagatgtg taaaggcaaa ttagaataaa ggcaaattag aatggcggaa
     3361 tcaagcaata tcattcctgt tcccatagaa gaagaagtca agagttctta tctcaactat
     3421 gcgatgtctg taatcgtctc gcgcgcgttg ccggatgttc gagatggact caagcctgtg
     3481 catcgtcggc ttttgtatgc tatggatgaa cttggacttc gcagcaacgc gacaaccaag
     3541 aaaagtgctc gcattacagg cgatgcaatg ggtaaatacc atccgcatgg agatcagtcg
     3601 ctctacgatg cgcttgttag aatggctcaa cctttctcat tgcgataccc acttgtacag
     3661 ggtcagggga actttggttc tatagacggg gatcctccgg cggccagccg ctacacagag
     3721 gccaagctct cgaaaatcgg agaagagatg ctcgctgacc tcgacaagga gaccgtggat
     3781 tttgttccca actatgatga aagcctgaaa gagccaagcg ttttgccgtc tgctattcca
     3841 aatctgctta taaacggctc gagcgggata gccgtaggta tggcaacgaa tatgcctccc
     3901 cacaacttac gcgaaattgc gctcgcaata gaggccttta tcgatgatcc agacataact
     3961 attgatgttt taatgaaaaa cattaagggc cccgattttc ctacaggggg tattatctat
     4021 ggcttgcaag gcattaagga cgcctacgaa acgggtcgcg gcaggttgac ggtgcgagga
     4081 cggtttatca tagagacgat gaaaagtggc cgagagcaga ttgtttttac agaaattcct
     4141 tatgctctca acaagacaat gctcgtaacc cgcattgcgg agcttgtgcg cgaaaagcag
     4201 atcgatggaa tctcagacct tcgtgatgag agcgacagag aaggaatccg catcgttctg
     4261 gagctccgaa aaggagccat aacaaagatt gtgctaaatc agctattcat gcgaacgcct
     4321 ttacagagca cattcggagt gatcaacctg gcgctagtgg atggcgtacc aaaatgtctc
     4381 aacttaaaag agctaattta ctattttgtg cagcataggt ttgaggtagt tactcgccgt
     4441 tccaaattcg aactcaaaaa ggccgaagag cgtgcacaca ttcttcgtgg cctggtaatt
     4501 gctctaggaa atatcgatga ggtagtagca ataatcaaat cgagcaagaa tatcgatacg
     4561 gcaaagactc gcttgtccga acgcttcgaa ttgactgaag ctcaggctca ggccattgta
     4621 gatatgcgac tgggaagact gacaagtctt gagacagaga aaattctggc ggaattaaag
     4681 gaaatcgaag ctcgaatcga gtatctgaag ggattacttt cagacccaaa ttccatccgg
     4741 caggtgatca aacaagaaat ccacgatctg gcagaaaaat acggcgatga ccgacgcacc
     4801 gaaattgtgc ctaaccaagt agagcagata aacattgaag atctaatcaa accagaagag
     4861 atggttatac tcatctctaa caagggcttt attaagcgca tctcggtgaa tcaatacaaa
     4921 tctcagggaa gaggagggaa gggctctaat tccgctgctt taatcgaaga agactttgtc
     4981 gagcagctat tcattgccaa cacccacgat tatatcctgt ttatctcaag ctggggtaag
     5041 gcctactggt taaaagtgct tgagattcca gagaccagca agcaagccag gggacctcat
     5101 attcgatcgc ttcttgcgat ttctgaagac gaggagataa gcgcaatcgt cgattttagc
     5161 gatttctccg aaaaggaata tatattgatg ggcacgctca aaggagttgt caagaaggtc
     5221 gcaacaagcg aatttgcaaa tgcaaaaaca cgcggcataa tcggtattac gctcgacaag
     5281 ggagacaggc ttatatccgc cgttttgaca caaggctccg acgaagtggt gctgatatcg
     5341 agaagaggac aggcgctgcg aattcatgaa tctcaggtca gaaagatggg tcgtaccgcc
     5401 cgtggtgtga ggggaatatc gcttcatgaa tcagatgagc tagccgctat gttgcgagta
     5461 gagcccaatg aatccatgct gatcatcact cagaatggtt ttggaaagcg tgtcagttac
     5521 cagcttttca atgtgcattc gcgtggcacc aaagggcaga cgatctattc gccagatgaa
     5581 ttctccggtg aagtggtaaa gggcgtgtct gtgcaggaag aagatgaggt catggtcata
     5641 acctcaacag gtaaaacaat aaagctcagc gtttcctcgg tgcgccagat ggggaagggt
     5701 gctcgtggcg tacggattgt aaatattgag ccgcccgatt tcgtcatagg tatagacaag
     5761 atcgttcaag aacaggaata ggaaatcggt tattgccttc tttactatgc agaatggcta
     5821 tattgtttca cgtgaaacaa tatgaatcgg agtgaatttt acttcactat gtttatgcat
     5881 gagaaggctc tcttatgcca ttcccaatcc tcataaacaa ggttcgtagc agtatgattt
     5941 tttaactaga tcggctgccg gaatattgaa taaggccttc taatagagaa atggtctttg
     6001 attctatcga tggtaaagcg gacaactcat tcaggtcagc ttgagccttc gatagatatg
     6061 aatcgatcat tttctgaata tctgcttcta ctcccttggc catgacagta ttgcgtatcc
     6121 attcaatgtc cttcaaatct tgcgttggag atgcaaagat agaatcaaaa cgctctcttt
     6181 ctcctttttc cagcctgttt gccaaaagga tgatatatgg agtcttcttg ccttccttaa
     6241 ggtcggaacc aacgggcttt cctgtctctt tctcattgcc gaataacccg atcctgtcgt
     6301 cttggagttg aaatacaatg cctatggatt ctccaaagtc ttcaagcaaa ggaatgatcg
     6361 atttatccat gatttgcgct gtctgagatg ccgaagcccg agccaatgaa gattgggcta
     6421 ccaaaactcc cgcgactaga ggcaaaacaa tagtatagcg cgcggtttta tggcgatata
     6481 cttctataac ttcgtcgata tccggaatgc ttttgtcata tcccaattcc acatctcgaa
     6541 tctgtgctat agtcaccagc gcgagttctc ttgcgaatag agaagatata tctagatcga
     6601 gtccagataa atcgagacca gaaatttctt gccaaccaag aaagaaaaac agatctccca
     6661 cgcagatacc ttgagcctcg gctatggaaa aggcatgagc tgcagcggaa ttggcgtgta
     6721 ttttttcgag aatatctttt atccgtaggt gaaaagttgg cttccctcgg cgtttgtggt
     6781 cgtgatccat aatatcatca tggacaagca gccccgcatg caaaagctct aagcccgcgg
     6841 cagcggatat agccttatgg tcttcataat cggctacaag tatacgttcg tcatgtaaaa
     6901 gtcgagatcc cacaacagcg agcatgcctc ttagcatctt tcctgaacat gcgaaatcct
     6961 gcatctcatt ggatagccac tggcccaatt caggcaaatt gacgaacaat ttctcgtttt
     7021 ttagaaaaat ctccgccatt ctctcagaga tatgatcttt gcatttttta gcgaaatcat
     7081 gaaagcaaat ctcatcagat gtttgcataa aaagcagtat agcgcgatga gatgcactaa
     7141 aaaagccctg gctgaatacc aaccagggct taagacatca aactgctagc aaggctttaa
     7201 gccacatagt cttggagcat ttccatggtc tcgggaaccg ccactttctt taaggctgct
     7261 ttttcaatct gtcgaattct ctcttttgta agatgcatgc gatctccgat ctccttaagg
     7321 ctcataggac gcttaccgtt cagcccaaag cgatattcca cgatctcacg ctctttagcg
     7381 gtcagggttg aaagaacctc gttaatatca tctcgcaggc ttgattgaat agcattttgt
     7441 tcggggcttt catgtttttc attctcaatt aactcgccta cagttgtgga attcctttcg
     7501 tcatatactg gagcatcaag cgatatcagg tcccgcgaaa tagatatgat atcttttata
     7561 tgctcgggtt ccatatttag gaatctggct acttctcgta tctcatcttc ttcggaaatc
     7621 tcgccttcga acaacgaacg agccttttca atctgcacaa gttcattggc tctatttaga
     7681 ggaaggcgaa tcatgcggct tttctcgcag atcgctttga gaattgcttg gcgtatccac
     7741 cacaccgcat aagatataaa atgatagcct tttgtaacat cataatgatc gatcgcgctc
     7801 agaagtccta tgttaccctc agctataaga tcagcaagag gcaatccctg gttttggtat
     7861 tttttcgcga catttacgac aaatctgaga ttgcttttgg ccaatatctc ttttgccttt
     7921 tcgtcgcctg cggctgcacg agtcgcgtat tcattttctt cttcgcgaga aagaagcgga
     7981 attctgttta tctcgcggag atacatggac aacacattct cgtcgtcata atgcgcactt
     8041 gaacgtgttt cctttagttt cttgttttgt accatcaaga tctccttgtc tatttaataa
     8101 gcaaaaagcg tgccaattca taactaagtc cgcaatttgt tcagatataa gtacatatta
     8161 tgaagcaatt catggtggcc attctaaaag aggagcttgt ttatcgatgc atagagcgaa
     8221 tatgtatcgt tttgatatac tagtctctaa agcaataagt attgtatcaa attgaaacac
     8281 tttctttttt cagagctcgg aaatcccgcg ggtcctccca cagcactgca tgaacggcaa
     8341 gccggatccg caagagcaag gctggatata agccatgctg gattgcagaa actggcgttc
     8401 tttctttctc tgtaccattg aatcaggagc aaaagctgga aattcgatct taagcttatt
     8461 gaaaagcatt aatttttcgc tgcaatcgag agactcgaac tcttggctgt ccttgatact
     8521 cttccaagca ttgagcaaaa aactgagaat tatcgagtgc tcagcctcag agctaaaacg
     8581 atagttcggg cgcttgacca aataaaagga gagctggata cttagaaaag catatagatc
     8641 atcgagttcc tttgttggtt gcttgatcat gccatatcca ttgacatgtc ggacatagaa
     8701 cctgcgtata tagccctttc tattcattct ttgagcattt gcaaggcttc ttactgcaag
     8761 gtcgcgctta ttcagctgca gaagagcaat agacaaccag taaaaggccc tagaaagctt
     8821 tttttcgcag gaaggaggcg taagttcgat cgactttcgg aataatccta gtgcttttgc
     8881 tggcttgtgt ctggaaagct cgtgaagacc tcttcttaga tatgagttag ctttttcatt
     8941 atctgatgat gttctcacgt gttataagtt attaatgaaa tttcataaag gcaagttacg
     9001 atccgagaca ggctcgcata tcgaaatcag caagataaat gatcgtggcg ccataagata
     9061 tttctgctgg tctaatagaa cgggctgctc tttctcttcg atttcatgtg gcggtggcaa
     9121 agaggtgtct attaccctct tccaaatatc atgttcatta tcatgaggaa ggcaaacctg
     9181 aatggcctca gtcgaagcgt tgaaagcaag aagataagaa caaggcagct ctgacgacga
     9241 gaaatgttcg ttaatacgcg cgacaagcac cttgcctatc ttgttccact tcggagcctg
     9301 gccgttttca tcataccaaa gtatatcggg aggagtttct gtactagaga gcctgtctga
     9361 tttggcttta ttttgtctag actgcccatc ggcgctaaat gtaccgatgc ttctttttcc
     9421 cacacctcta aaaaaatcct ctcttctgaa tgcatcgtgg tttttgcgaa gagcgatgag
     9481 actcttgacg aaacgataaa tgtccttgtg cttttttaga agactccagt cgtaccaatt
     9541 gagctcactg tcgtggcagt aggcattgtt gttgccattc tgtgttcgac gaaactcatc
     9601 gccaccgaga atcatgggcg tgcctatgga aagcaagagt gttgcaaaga aattcttgat
     9661 ctgccgattt cgtgtttctt cgataaaagg ggcgtttgaa ggtccttcgg tgccatagtt
     9721 aaaggagaag ttttccgcta tcccgtcgcg gttattctct ccatttgcct cattatgctt
     9781 ataattataa gatacaagat cattcatagt aaaaccatca tgcgacgtaa caaagttaat
     9841 cgaatggaat ggtcgtctgc cattattgag atagaggtcg ctcgaaccgg taattcttgt
     9901 tgctagaagc ggagcaaatc cttcatctcc tctccagaat cgccttacat catcacgata
     9961 tcgatcattc cattctgccc atctcccacc agggaaagat ccgacctggt aagctccacc
    10021 tgcatcccag gcttcggcaa tgattttcgt gtctcggaga attgggtctt cggcgataga
    10081 ttcgatgaca gggggattgg ctagcagatt accctgtgaa tcccggccca aaatcgaacc
    10141 tagatcgaat cggaacccgt ctacatgcat ggtaacgacc caataatgca ggcattccat
    10201 tatgaaggtg cgtagtacag gatgattgca attcagggta ttcccacaac ccgagtaatt
    10261 gcgataatag cgcttatcct ccgccaacat ataatagatc gagttatcca gtcctcggaa
    10321 gctcaaagtt ggccctagct cattcccttc ggcagtatga ttgaacacca cgtccagaat
    10381 tacctcgagg cctgccttgt ggagctcttt taccatgtac ttgaactcct ttacaggatg
    10441 agaggggcaa tagctatcgt ggtcaatacc ctcatcgaag gcataagaac tctccggagc
    10501 aaagaaagcg atcggagaat acccccaata gttcgaaagt ctctctccag taataggatt
    10561 tcgcctaaat cgctcggaag agtcgaattc ctgtaccgga agcagctcga ggctcgtaat
    10621 accaaggctc ttgaaatatg ggatcatttc gaccacgcct cgataagtac catgatgctt
    10681 tacttttgcg gtaagagact ttgtcaatcc tcgtacatga gtctcatata tgatgcaatt
    10741 cttaagtgga tagtttaagg gagtgtcgtt ttcccaattg tattcatcat ccacaacgac
    10801 gcactttggc atagaaccat cgtttggaat tgtcgaaagg gaaaggtctt tatccggcga
    10861 ttcttcatcg taggcaagag cggaacagag ctccaaagaa ccatcggtaa tggctttagc
    10921 ataagggtct ataaggactt tatagggatt gaagcgatgg ccttcgtgtg gagcaaatgg
    10981 gccttccgcg cgccatagat aaagaacctc tgactcaaga ccaggaataa agcagtgcca
    11041 gacatctcct gtgcgattgc gcattgggtc tagctgcaga gaagctgcag gaatatcgtc
    11101 atggggcttt tcatataagc agagaatcat acccctggcg tttctggaaa atatcgagaa
    11161 gtacactccc tctgccttgg gaatagcacc caagagccca ggattgcctg gcatcagggt
    11221 tagttttcga ggtagtgact tcaatgcatc attcatggtt ttgttatgac tagaactgcg
    11281 ttttgtccgc caaaacccat cgaattcttg ataaaggctc taaccggcat gtttatgccc
    11341 ttgtggggca cataatccag atcgcattcg gggtctggat tatcgagatt tatggttgaa
    11401 tgcacataat tctcgttcat accaagaaca gccgcaatag tctcgagtgc accggcagcc
    11461 ccaatgcagt ggcctatcat ggattttagg ctggaaatct ttaacttata tgcatgggcg
    11521 ccgaacgcat tctttatggc ttttgtttcg atagggtcgt taagaggtgt tgaagttcca
    11581 tgggcactaa tataatcgat atcctcaggc ttcatctgtg catcgttaag ggcaatttcg
    11641 attgccttgg aaacccaaag accctcggga tccggggctg tgagatgatg ggcatcgcag
    11701 gtagaaccaa agcccgctat ctccgcatat attttggctc cacgagcttt ggcatgttca
    11761 tattcctcga gcaccaggat gccagcacct tcggacatca caaagccatc gcgatctcta
    11821 tcgaatgggc gcgacgcctt ttcaggctca tcgtttcgta ccgaaagagc ttgaatattt
    11881 ataaagctag ccatgcacat acgagctatg ctagcctccg agcctcctgt aaacattata
    11941 tctgcatggc catctcggat aagatgcata gcctgcccga ttgcatcagt tgctgcggcg
    12001 caagccgtga ctacggtcat gttgggccca tgtacaccca gagatagagc tatctgtgca
    12061 gcaccaaagt ttgcaaggcc ttttgcaaca gtcattggag gaaccctatt gggccctttc
    12121 tctgcgagac gcataataga ttcttctatg gtgttggttc caccctggcc ggtgcctaaa
    12181 cagactccag tctttattgg atccaaatat tctctaggaa tattggatga ctccaatgct
    12241 tcaagggctg catagacggc aaattgagcg aatcgatcca tttttttggc atcttttggg
    12301 tccatttttt ttgacggatc gaaattcttg acttcgcctg ctatcttaac tgcaaggtct
    12361 gatgaatcta tcagcgtaat aggccctacg cctgatctgt tagctttgac attctgccaa
    12421 aacgtaggaa catcatttcc aaatgggcta accaggccta ggcccgtgat aaccactttt
    12481 ctttttgcca taattacatc cccatgccac catcaacatt tagcacaaca cctgtaatat
    12541 acgctgcatg gctgctgcag agaaagaggg cagcctctgc aatatcttcg ggaacgcccg
    12601 gacggccgag aggaatggaa gactttagat tttcctttgc tgtttccggt aatacttgag
    12661 tcatagatgt ctccacatag ccgggcgcga ttgcatttac acgaatattg cgtgatccaa
    12721 gttcttttgc gaggcttttt gtaaaaccta taagaccagc tttagatgcg gcatagttgg
    12781 cttggcctcc gttaccatgc aggcctacca cgcttgacat gttgaggata cagcctgaac
    12841 gctgctttag catgatgcgc gagacggcgc ggctaagaaa aaaagcgctg cgaagattgg
    12901 cgttcaatac agcctcccag tcttcggttt tcattctcat gattagacca tctttggtca
    12961 tgccggcatt gtttactaaa acatctatgc gattttctat tttgaaaatt tgatcgatag
    13021 cggattccac agataactca tctgtgacat cgcatgaaat ccaatctata tcggcctctg
    13081 cttttgtgcg ggataaacca tagactatgg ctccttcttg tttgaatcgg gtagctatgg
    13141 caaggcctat gcctctggaa gcacctgtaa cgagacagac ctgccctgat agagtgcctt
    13201 ccataatatt tactccttat atttcactat attatcgaga caactaaagt atcgaacctt
    13261 ttggtcagat aatttataga gaaaaggctg caaggtcttg cagagtacca gctgcgatac
    13321 atggaataga tgagcctgaa gctttccaaa gacctgcaag aaccgttccc ggtccggtct
    13381 cgaaacaata gtcatagcca tccgcagcta tactcttctc ttcatctatc cagcgtactg
    13441 gcgaaatgat ttgaagcgac gcatattttt ttgcatcttc tttgctcagc atcttcttgc
    13501 ctgtaacatt ggagtagagg ttgatcttcg ggtctgaaaa ttgagtagcc tctacaagct
    13561 ccttaaacga ctcgtatgca tcctgcataa gtggtgaatg aaatgcgccc gaaaccttta
    13621 gtcttatgac acgcttggct cccgcctggc gcagcctctc ttccgctact gcaatatctg
    13681 cttcggttcc ggaaataacg cactggactg gactattgta atttgctatc cagcattgtc
    13741 taaggccagc ctcatgtata acctgttcta ttttttcggg ggagaggtat aacaccgcgc
    13801 tcatcgtagt gctgccagcg cgattgcctg cctcttccat cagtcttcct cgctctgata
    13861 caaggcgaaa catatcaaag ctgctaatga cctttgccac agcaagggct ggccattcgc
    13921 caacagaaaa accagccgca cctcctggca ttatgccatg ctctctggcc atgagagcag
    13981 aagctgcctc ggctacgacc atagaaatct gggtgtttct cgtctgcctg agcgtttctt
    14041 catctccttc gaagataagc ttcttaagat ccatggcagc agcttctgaa gcttcgttga
    14101 acagctcgcg gacagctttg cttgcatcat agaaatcttt ggccattccg gggtattgcg
    14161 ctccctggcc tgggaaaaga aagcaattct tcataagtcc tcctattagc ggcatcttcg
    14221 atagacatta atcattgtgg ttcttagtcc cttttcatgc ttatatttct cggccatctc
    14281 ttcataagca catattttac attcttacca gatttgtcat tgttttcaaa gaaccctgta
    14341 gaagatggtg ccagatcgga agaatccatg ttcaagggcg ttatcgcaca ataatgaaga
    14401 aaagaaaggc ccttcgagca aaatctgtgt tgctccgatt ctcgcacagt aatcagaaat
    14461 gcattcgata agaggccatt ctaaatccag cattgcactt tcttttgttg caaatacacc
    14521 gtgtaccaca gccatgagtg agcccctata ggtttgaagc aaaaacagcc ctgctattaa
    14581 gggtgaggta ccatcgattg taccaaatgc gacaaacagc gagtttttat cggttcttac
    14641 ttccttaaat aaatccgaaa gcgatcgcct tttgatagct atgggcaagg atccgaagga
    14701 ttcctctata cagcaatcga tataggattc caattcgaaa gcaatttcat cgggaccgga
    14761 tatcatgaag ccgatatcgg aagcgattat atctcgcaga tcatctggct cttctttgag
    14821 agccattagc atttctccct cctgaagcgt ttctagccac ctttccacaa tgggacgcat
    14881 agccgcgagc ttgaattcct ggtatcgcat gaagagttcc tggtctttgg atagcacctg
    14941 acggaattgt ctgaaaactc ccttacctct tctaagtgcc gcatttagct ctatcttgag
    15001 actagccggc gaggtaaccg ttgcagcaaa ggcttcgaga atggaaaagc cgcgatgaga
    15061 atcccatgaa ggcggttcca caaatcgctg gccaatctct agatatggct tggatgtatc
    15121 atcattttca agtgcggttt tgtctatgag atcccctgtt tcaagatcta tgaggaattt
    15181 tttcgactga tcttccatgg cgaagatgat tcgctccagt atggcatcgg tcagctgaaa
    15241 catagtttca ttctaatcga tattccttcg tctatggaag ggcaaatttc aatattgtat
    15301 actagcgtca aatgctttcg aacttgcatc tgcactcgcg cttttcggat ggaatgtcct
    15361 ggccggaaga tatcgccttg gaagcagcca gggctggcct ggatattgta gctctaaccg
    15421 accatgatac gatgggcggt actgaaaggt tcatccaagc ctgcgaggcc agatcgctga
    15481 aggccattca ggcttgcgaa atagatatat gtgagccaca gatcgagtac aagagcgagc
    15541 ttttggccta tttcccgaag gctgatctcg attcttcgat tctctatacc aaacgcctcc
    15601 ttcaaaaatc actcgagcag cgcagaagac ggttagaatt cttcattgaa tcggctcaag
    15661 aattatatcc agacaaggag ctttcttttc aggacctttt tagggacaag accggccttg
    15721 atttcgatga agagctagct cgtaagattt cgtggagcaa ggtagatttg ttcttgtatc
    15781 tgaaggcgca tcgctgcatc aaacccgaga tgaattacaa agaattcaag aagagatttt
    15841 tcgcaagcgg gcttcttaaa aagttcaagc ttgacaaacc cgatgttgca actgtggtca
    15901 aggcggtgca tggggacgga ggttttgttg tgattcctca tcttggtcat ttctgggatg
    15961 atgaacttcc tctgtttcaa aaagaagccg aaaaattacg acttcttctt gcatggttta
    16021 gacgcgcagg agtggatggc gtcgagttgt actggtacaa tagcgaattc aagagcgcta
    16081 gtttcaacga acagctaaga gaaatagcaa gaccacaggg atttttcttt acctatggtt
    16141 ccgactgcca tgggccgggt tcgggcaagc atacgatcat gaaattcaag gggccatttg
    16201 atggtttccc acaaaaaaag agcatatcat gataagatta tgtgcattcc ttgggaatca
    16261 gggacaggaa tatcgctatc ataggcataa tgtggcttgg ctttttcttg attcgatgcg
    16321 tatagcctcg gaactgcgct ggagttcaaa gttcaagggc gcgtttactc aaatagaatt
    16381 aggttcaacc attatttatt ttttgaagcc acacaccttt gtgaatttat ctggcgagag
    16441 cgtaggagaa gcagtccatt tctataaatt aggattggat gaaatactca ttgtccacga
    16501 tgagctcgaa atgccgtttg gcgcctttgg ctacaaattc tctggcggtc ttggcgggca
    16561 taatggattg cgctctttgg agaagcatct gggaaccagg gacttttgga ggcttagatt
    16621 cggcattggt agacccgaac acgatgatgt ggcgggatat gtactatcgc ccttcaatcc
    16681 agagcagctt gaggttctta ataagaaggt atttcctctt gcagaaagaa ctttttatga
    16741 gctcttttat gaggggatag aaggattcga tgtccgatat aagaaggtct tgccgccatg
    16801 aagcgataga gatacgctat cgtaatggcc agaaccaaaa catcaaaatc tcgaaatatc
    16861 aaaagatgat tctcgaaagc gcggaatcgc tctttctcca atcggggtcg accctgactt
    16921 gaagctgcaa ctttatcgga taatcgaaaa gctctttgag atcagcctcc gcttcttcac
    16981 gaatgcgttt tattatcgag ccttggcgtc ctatgactat ggccttttga gaatctctct
    17041 ctacaagaat gtcgaagcga gcaaagagaa ctccatcggc acgttttgat gattcgcgat
    17101 actctaccgc gatagcatgg ggtaattcct ggtatgtatt gagaatagcc ttttctctga
    17161 cgagctctcc tatgcgaaaa acaggctcct ggtcggtgta aatatcttct ggataccaaa
    17221 acggtccttc gggagcgagg gaaaacagta cctcaatcaa ggattcgaga ttcgtctttt
    17281 cgacagcgga aatggaaacg cgctttgctc cgcggaggcg aggtataatg aactcctcga
    17341 tcaatagggg cctggcttca ggatcatcga ttttattaat agcgacaact attcggtgga
    17401 tatctttcga aagaagctct gcaatagcct cttcctcctt gccaggttcg cgcgtcgcat
    17461 ctatgagata aagaatcagg tcgacttccg aaagagatct tagaacaatc tctcgaagcc
    17521 tcagattgag cttcttgtcc gatatatgat agccaggagt atccacaaaa ataagttgcc
    17581 cttcaggtcg ggttattatg ccacggatcg catttcgtgt cgtttggggg acggagctga
    17641 ctatagaaac ctttgctccg catagtgcat tcagcagcgt agatttgccg acagaaggcc
    17701 tgcctatgat cgcgacaaat gcagacttgg gcatttttgc ctcctttgca ttgttgaatc
    17761 atcttaaacc cttactcgcc tcgatagctg tatatactta tcgatctata tttgctcagg
    17821 ctgttcgagt tgagagtaga ttgcgggtgg aggttctccc atccagagct tatattgacc
    17881 agctgcctgg taacagagca ttttgaatcc attcatgatt cggcagcctg cagcttcagc
    17941 ccgctttaga aataaggtct tgatcggata atagactaaa tcgaaaacag cttcggaccc
    18001 tttgaactca taaaaactta taggatcgcc ctcgttttcc atgcctacac tcgtggcctg
    18061 tacaatcaag tcggaatatt ccaagaggag atcgatagcc ctgtcatcga gatgtccata
    18121 taggaagttg tattttcgcg ccagatcgcg tcctatacta tatgttctat tgagaacgag
    18181 gcccctggcg cccattctaa acagagaaag agcaaccgat ttggccgcac cccctgcccc
    18241 tataattgtc gccttacatc cggaaatatc ctgtttgccg aggaattcca ggagactgcg
    18301 ctcgaagcca tcagcgtcgg tgttatagcc cgcccatcct gattcgcgta agacaagagt
    18361 attgcaggct cctatgagtt gcacatcggt ggagtggaaa gaaagatatg gcagaatatc
    18421 ttgcttgaac ggtacagtaa ttgccgctcc tgtgcctccg aggatttcga gcatcttaag
    18481 cccatcttcg cagttgtcta cgggcgtggg aataagaaga gcattcttac ccatttttct
    18541 gaatgcgctg ttgtgcaaat atggcgaaag cgatttagcg atcgagtttt gacccatgag
    18601 cgtgtaaatc atcgtatcgt tatcaacaag gtcgaaattg tagatattga cgagatcgaa
    18661 ggggtcgata tgacctggcg cagcagcgtc cattccccct cgcaaaggac tcgcataagt
    18721 ccataatgag cctaaacgcc aagatagaat gcgcgaggcg ataccataat ctccgctagc
    18781 gatgaggatt tgatcacacc ttggtttttg cagacaccac tctagaaaac gaatgaaatc
    18841 tgcgctgttt tgaggattta cggtgagcct gggtatttcg tcttcttcag aagtcagttc
    18901 ttcataagcg gcatcgagac tgtccggcat tccaccgagg cagtggtgag atcgaataat
    18961 acgagtacca aatgtgcgac aggattcttc caccactggc gccctaaaat catcttcgag
    19021 atcaacatag gcataattag ctcgagcatc gggctttgga aaggagagtg cccttgctat
    19081 gagaacaagc cgagtacctt ctcctccttc aaacttgcca ccatctttca gacgccttat
    19141 tgttaagatg caagggagac cggctttctc agggaagtca cgaataaaga accgctcact
    19201 tggatcaagg caatccgcac gtagctctac acaatctaca actgaacgat attgctcaag
    19261 gacgcgcaga tcctcttcga ttgtcttgcc cgtcatactg aggcagatct ttggcctgtc
    19321 gtttttctcc atatttcctc atcggttgga agcttgtata acacttcctt attgttatgc
    19381 agcgaggctt tcttgtctat agtttctaat actatagttt acatagtggc aaagactgaa
    19441 gtatattagc gcatataagc cattattcca tcaaatagca ggagttgatt cgtgccggac
    19501 aatagtgatg caataacctc cagattcaac gaaaaagctc taaaagcctt tctcgatggc
    19561 ctgctcgatg aactcagaca tccgcaggac ccgaaacttc tcgaagacgt tcgcagagca
    19621 ttcagaaaaa agattcccct ccatctccga tcttatgccg cagcactcat gatccttcgt
    19681 gccgcaggcc tatccagggc aaagccacag cagaagatgc cgcaacaaaa agcgtctccc
    19741 caaaaaagtc caactccaaa agtaccgcct cagccccaaa aaaaatcagc ccgaaacgca
    19801 aattggcaac aggggaagag ctcatttaca gcagttcctt caacttcggc gtccattagg
    19861 agcactcctt ccagcacaag ctcctccgat tcaccatcct caacgcagga actgccatcg
    19921 aaggagacag tatctctttt tgtctccatg gggaaacgac accacttaaa accccaggag
    19981 ctgagaaatg agatagcaga gcacacgggc ctatctctcg atgaaatcgg gcgaatccac
    20041 ctgctcgaaa aatactcctt catagaagtg ccggaaaaag aagcgcaaaa ggttattgcg
    20101 gcaatgaatg gtgcagagct aaatggccat gttctcgaaa taaagccggc aaagaagcgc
    20161 gcaaagacat ctttcagagg ataataggac gtggcttcga caatctggcg tcttaaagtg
    20221 gggctaatcg gagaaaattg ttatatcgtc gaatccgagg gcaaaatact cctcatagat
    20281 cctggtgcag aggccgaaaa aatagatgat tttatgagat ataaagggat taagccttct
    20341 cttatcatat tgacacacgg ccatctcgat catacggcag ctattcccga tcttttcgaa
    20401 atatggggga cggagcttcc aatagctatt catccgcaag acgccgctta tcttggaaaa
    20461 gatagcgagc aaaccaaccg cctgcttttc gaagcaatta aagctccatc ctattttcat
    20521 gcctcctgga aaaccatgcc gcctccgggg attttcttga ccgatggcat ggcggtgcct
    20581 ggtaccagtc tacaggttct tcatacacca ggtcacagca ggggttctat ttgtctatat
    20641 gaacctgaac ttgaggctgg ttcgtatggc tatgaagaaa agagcatggt gcaaggggct
    20701 aatccttcct gcatcatctc tggggacact ctatttcgcg atggagtagg acgcacagat
    20761 gcgccggatt cagatgcata tgccctagaa gctagcctta ggagacttgc gcgtttctcg
    20821 ccggatacgc ttgtctttcc tggacatggc cctcgaacaa ccataggccg agaactcagc
    20881 tgagcattat gtggactgag cattatatag actgagcatt atatggcgcg aacaaattgc
    20941 atttgctgcc ctgggaatag tttcaaatga aattctccat gataaagcct ttaaagatgg
    21001 aatcttctaa attcatctcg tcgcattttt tggtgattag ctttgtactc gcatgcacta
    21061 ttgcgtcaag accttctttt ctctatgcaa aagagttgag caaaactgat tttgccctcg
    21121 gcacgctctg cacgatccgt cttgtcgaag gaggaaacag cgctacatta gaccaggcct
    21181 ttctgcgctt gagaacaata gaagatcgca tgagtgtgaa caaagagggg acagaggttt
    21241 cgaaaatcaa tgaaatggca gggaaggctt cggtcgaggt ttcgagcgat accttttatg
    21301 tgatttccaa agccatcgac tatgcaaggc tcaccggtgg cgcattcgac cctagcattg
    21361 gccctctaac aaaactttgg aatatcggcg cgggcggaga aaaagtccct cccaaagaaa
    21421 acatcctgtc tgccaaagct ctggtcgact ggcgcaatgt cgtcctcgat gcatccgcca
    21481 ataaggtttt cttgaaagag gagggaatga gcctggacct gggcgccatt gcaaaaggat
    21541 atgcagctga tgaaatcgaa aagattctgc ggctggcaaa ggtcaaagca gcagtaatcg
    21601 atcttggcgg caatatcttt gtatacggaa ctaagaaaga caaaaccctc tggcgcgtcg
    21661 gaatccagaa tccggaatca gagcgaggag attaccttgg cattgtggaa ggcccccaga
    21721 tgaccatcgt aacatccggc atctatgagc gttgcttcat tgaaaatggc aagagatacc
    21781 atcacattct agatacaaag acaggattcc ccgtggataa tggcttagta tctgtttcga
    21841 tcattggcca gagcagcatc gatgcggatg cgctttcgac ttcgctaatt gctttaggca
    21901 tcgaaaaagg cttggaatta ttgaaacgat tccccaatac ctatgcgata ttcatcgata
    21961 aagacaaaat ggtatatctt agtccgggcg cggagaaagt gtttacgctg ctggataaga
    22021 attacacgct tgtacgatga gccagcccca cttttaagaa taagaaaggg actccaaagc
    22081 taacccagcc accaacaagc gcatagcgca gaaagcgaag gaggttttcc tgattattgc
    22141 ctaagaggtt attgatagga ccgcttgccg attttagcaa gacgtacaca attccagtcg
    22201 tgatcaaccc tacgagatat cgggaaatct tgatcttgag tctatccttc tcgttgaaag
    22261 cgagactgcg cgctgcaaat gtaaaaccga tagcggcgcc aaagaaagct cctgagatca
    22321 tcgtctcttt aggcagaatg agattcatca caaaagaaag tgccgcggcc aatataacac
    22381 gcccgcgcac gtctgccttt tggagaattt tttcgattga aggtccgccc agcttataca
    22441 ggataaaaaa gagacttcca gctagatagc ctcctagaac atcggttaaa aaatgtacac
    22501 cgaggtatat cctcgaaaat ccgataagaa gggggaagcc tatgagcatt agaggtcgcc
    22561 attttttggg taagaactgt ataatataag cggcaaaaac tacactcagc tgggaatgac
    22621 ccgaaggcat accatagcta gtttcgcttg caagtcctac gcttgggatg atatcgaaag
    22681 gccttggcca tccgaaaatc tgcttgagcc agagattgat aaaagtagaa accagcacca
    22741 aaaggccaag ctcagccccc ttcttgcgat ttacacacca gtacacaata ggaactatcg
    22801 cgattacaaa ggcttctgat gcgaataatg atacaatcgt catagggatt cgaagaccaa
    22861 gacccagaaa agtctgaatc tgaaggataa tctgcgcttc ggcagcatgc atcgattcta
    22921 cagacatcat aacaataact ataatcgcag caagcgaaaa aataaaggat aaaaacgcat
    22981 cagggatccc caattaagag aaaaacttaa gaaaagcgtg caaagacctg gcgagccaag
    23041 gcaaaaagcc tagaatcagg gctcaaaagc atcaggaaaa tgcggctcgc tatctgttat
    23101 tacgcatgat tgaggaaaac taagagctgc ctttgctgta gggtcttttg caaagagaga
    23161 aggccacatg gcagctttag caaaacgagg ctcttcagaa cgcttttctg caatgcgggc
    23221 ctctagctca tccattgatt ttattatcgg attcgagaag gtaagaagat ctatcgcatc
    23281 agaggaaaac tgaaaggacc acagagtccc atacatgggt acgaaagcaa aggcagtgcg
    23341 gactatatgg aagacatctt taagagtcct atagatacat gcccagccta taggtcttgc
    23401 gatggatgat tcaccgtgca ttgcaaatat gccttccgga cccttcaata ttcgttgaac
    23461 ttgcatgaag aattctttgg tatagagccg ttcggaaggg ccgaaaggat cggtcatatc
    23521 cataatcaca acatcgaaag aattatcagg acattgctct acaaaagccc tgccatcgcc
    23581 gatttcgagc a
//
LOCUS       DAOMZL010000022        22853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_1931_length_22853_cov_14.586323, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000022 DAOMZL010000000
VERSION     DAOMZL010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 22853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22853
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1931_length_22853_cov_14.586323"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PLQ30_03205"
     CDS             <1..411
                     /locus_tag="PLQ30_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HPW01483.1"
                     /translation="YIRPSPIDADIGAQLELLRKAGVESIFEEKPLSKEADRPELRRA
                     LDSLCNGDTLFVASLDRLARTITELFYILEEIRRRGANLESLKEQLKIEDDFIRHLGI
                     IIDFERISLLEWQRGKIEAKKSWRERGAGSKRQR"
     gene            complement(447..1091)
                     /locus_tag="PLQ30_03210"
     CDS             complement(447..1091)
                     /locus_tag="PLQ30_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HPW01484.1"
                     /translation="MKVGQRMTRNPVTITPDTTVPEAQAIMRREKIRRLPVLDKNGKL
                     VGIVSALDLTHASPSPATTLDVWEMHYLLSKLTVDKVMTRDVITITEDLPIEEAARIM
                     DDNQISGIPVMRGEILVGIITESDIFRLFIELFGARHKGIRLTLLLPERKGELAKVSN
                     AITKCGGNIVSFATFEGEDPTNAYCTIKVTGVEEKTLIEAITPVVQKVVDVRET"
     gene            complement(1165..1878)
                     /locus_tag="PLQ30_03215"
     CDS             complement(1165..1878)
                     /locus_tag="PLQ30_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW01485.1"
                     /translation="MALLELKDVRTFYGNIQALKGVSISVEEGEIVTLIGANGAGKTT
                     TLMSICGIVPIRSGEILLDCQDISRMSPHKIVQRGVSQVPEGRRIFPQLTVSENLDMG
                     AFLRNDREGIKRNMEEVFTIFPRLAERRNQLGGTLSGGEQQMLAISRALMANPRLLLL
                     DEPSLGLAPLIVQNIFEVIQKINKERNTTILLVEQNANMALKIANKGYVMQNGLIKIT
                     DTAADLLKNQEVRRAYLGL"
     gene            complement(1880..2680)
                     /locus_tag="PLQ30_03220"
     CDS             complement(1880..2680)
                     /locus_tag="PLQ30_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW01486.1"
                     /translation="MSTEKLQYYNEILEIDNLVMTFGGLRAIDGVSMHIDEGEIAALI
                     GPNGAGKTTIFNCITGVHKPTEGKVKIRNRDGRVEEIQGLKPNVINCKGLARTFQNIR
                     LFNNMTVLENVMIGRHNSLKAGVFAAIIRNAITREEEQRVIEESYTILKKLKLDTYVN
                     ETAKNLPYGEQRRLEIARALATDPFMLLLDEPVAGMNAQETKELEETINIIRDQEHIT
                     ILLIEHDMSLVMDVSERIYVLDYGRLIAEGNPHEIKKNPDVIKAYLGE"
     gene            complement(2677..3948)
                     /locus_tag="PLQ30_03225"
     CDS             complement(2677..3948)
                     /locus_tag="PLQ30_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPW01487.1"
                     /translation="MKARSILKDIGKSLIIAVWFMILFFPIMVMKVNVTRGVAHINFR
                     WNVLPLIGIGAFVLSFVWTKALEWNERRTSKKKIENGLINNRALNGRYLKNPALRPLI
                     LLLVFGFTVAFPFLFGMYHTNVMITAFIYVILGLGLNIIVGLGGLLNLGYAAFFGIGA
                     YTYGLIWKYIGPSFIAAGFNPGWLFWISLPLAGIAATIFGVILSLPVLRLRGDYLAIV
                     TLAFGEIFHMVMQNAGDLTGGATGISLIPRPWFLGQKLQPSEAATYIYFIALVFVMIT
                     IFVVRRIEDSKVGRALEAMREDEVACEAMGIDMVKNKLITFAVGTFWAGIAGVLMASQ
                     TTYINPDSFTLWESIMILMAVVIGGTGSIPGVIAGAILLKLLPEYFRPLAQYRMLIYG
                     IAMILVIIFKSDGLIPRKRKQYTFTEKELAK"
     gene            complement(3952..4854)
                     /locus_tag="PLQ30_03230"
     CDS             complement(3952..4854)
                     /locus_tag="PLQ30_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967890.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HPW01488.1"
                     /translation="MEYFIKLFLSGTAKGSIYALMALGYTMVYGIIQLINFAHGEIYM
                     IGGFTALILGGFFFSKGMPVSLVLVFSILLSIIYAAAFGFTIEKIAYKPLREKPRLSA
                     LISAIGVSIILQNFVLLSQTEKFLPFPNYLPEFSWLLPYRSYINSTQLIILITTAIIM
                     VLLTLLIKFTRMGKAMRATAQDMHMAQLVGVDINQVISLTFVIGSSLAAIGGVLICSY
                     MGQINYYIGFIAGIKAFVAAVLGGIGSIPGAVLGSFVLGWTESFGTGYVSSDYEDAFA
                     FLILIIILLIKPDGILGRSRRQKV"
     gene            complement(4944..6062)
                     /locus_tag="PLQ30_03235"
     CDS             complement(4944..6062)
                     /locus_tag="PLQ30_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HPW01489.1"
                     /translation="MKQRKMLFAAIVSLVLFLGAQAVFAAPTIKIGVAGAHSGDLASY
                     GLPTVNAAKLVVKEYNDKGGINGQKVELIIEDDQCKPEIATNVAAKLVSTKVAAVIGH
                     ICSGATKAALGIYKDSRLVCISPSATTPFLTQSGEYPNFFRTIAPDDAQARLATDFLV
                     KDLKVKKIAVLHDKGDYGKGFAESVREFAEEMGVKVALFEGINPGAPDYSAVINKVGN
                     AKVDAVVWGGYHPEASKLVQQMKEKGINVAFISEDGIKDNTFIEVAGKYAEGVYATGP
                     TDTSANPMANKAIEAHKKEFGSEPGAFFLNAYASAQALLNAIEKAKSTDYDKIVAALQ
                     SEYVDTVLGRITFDKKGDIVGYGNSLFQVKNGKFIVLK"
     gene            6450..7442
                     /locus_tag="PLQ30_03240"
     CDS             6450..7442
                     /locus_tag="PLQ30_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW01490.1"
                     /translation="MSNRFAKLLYAIGCAGILLLLSCAPKGAKVVGVAKFVSHPALDA
                     LEKGILDELSASKADYKVDLQNANADMNTAAQIAQHFKQEKVALAVGIATPMAQALAN
                     QIKDRPVIYSAVTDPVAADLVESMDKGGPNVTGTSDMTPVREQLDLLCSLKPSIKRIG
                     NIYASGEANSVALAEIVREYCQEHGLEYVESTITNSSEARQALLSIAGRIDGLYLGND
                     NTVFSALSGIAEVGIDKKIPIVTADPSSAETIPVLAALGYDYYRMGLATGKIVVRILN
                     GEKTSDIPAFLSKENEDMTFVLNLDTARKIGISVDQSIIDRAKVVISDDQLVRK"
     gene            7446..8354
                     /locus_tag="PLQ30_03245"
     CDS             7446..8354
                     /locus_tag="PLQ30_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW01491.1"
                     /translation="MVEGILVEGLIYGILAFGVFVSFRILDFPDLTVEGSFPAGAAAG
                     AVVASAFNLSSNAVLHGLAVPAGMLAGFVVGGIAGFATAAVYHKLRIPPLLAGIVTMT
                     GFYSINLRILGGKPNYPLITNNPMLKLARSFLGGALPSEWSLFASCVLVCLVLFGLLD
                     LFFHTEIGIAMGALGDNENAVIQAGIQPNRLRTWGIMLANALPGLSGAMAAAYQGFAD
                     VNLGQGVVASGLATVMLGELIIRSQFISVQLLRVLIGSILFRALMYAARSWGYVAGIT
                     PNDLRLLTALLIILSVALSRIKRKRS"
     gene            8382..9173
                     /locus_tag="PLQ30_03250"
     CDS             8382..9173
                     /locus_tag="PLQ30_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW01492.1"
                     /translation="MVRIEDIVVSFDLGGGQEHTVLNKLSLEVKQGQTVSIIGSNGAG
                     KSTLLNSIAGSIPLHAGHIHLNGEDVTSWAEWERARYLGRVRQDPMAGTAGEMTVIDN
                     LALAARKGPRRFRIATPPRFAREMERKLAELGMGLESRLRENVSRLSGGQRQALTLLM
                     AVISHPTILLLDEHTAALDPANAEIVDALTRRFINEYDLTALIITHDMKRALEQASRV
                     IMMHEGEIIADLSGPEKEKMNVAALVRLFKKVRGSEYAEDRDLLI"
     gene            complement(9239..10630)
                     /locus_tag="PLQ30_03255"
     CDS             complement(9239..10630)
                     /locus_tag="PLQ30_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPW01493.1"
                     /translation="MKLAFLHQSTELRRDYILNGPLSSTLMLLAAPTLMLGVVQALMP
                     LMDGLFINNIAGTLVASSVTFSEPVINMTTALAQGLSVAAMAIIGQLNGRGDLKESKR
                     TSTQILVMGSMLGCISAPFLVLAAKIISSRVNPGISHNVFLYLSLYALVLPFSFMESI
                     YNGIKNANGMPEAPFIRMVLMLILKIAFNFLYIYEFRMGIVGCVLSSLSANIIITLWM
                     FWELFLKKGPDRLELKGFKFDPVAAKQLFRIGAPAMANTFILNLGFFLINTEVEKYGP
                     VVLNGQGIANNITQVAFNVPSAFGSAVTTMVSMNIGAENPRKARESMVHGTTLSALTA
                     AILIAIIVPLSSRLTVLFTRRQDVLEIANRALHIYTYSVIGFGITTTIQGAFIGLGRT
                     RVPLVLGILRIWLLRYAFILVTEKYLQYYAVFWGNLFSNYASAIIAIILISRTKWESA
                     IGKSMQLEVASAK"
     gene            10932..11636
                     /locus_tag="PLQ30_03260"
     CDS             10932..11636
                     /locus_tag="PLQ30_03260"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPW01494.1"
                     /translation="MEESDNIIIEKRSLAEQVYLYLCNSISNGKMKYGETINTRRLAE
                     ELNISMMPVREALKRLETDGIVEIKPRSMCLLRTPTRETILDAIAAREMLEIYSVKSI
                     YASIENSRLDVLRKIIEEMKMAISEDPIDLRKYIKHDWQFHKELCNLVDNQFVNKFYK
                     ELNIHLNMSYVYDIGIEPNVSQTFRDHIDLVNALAAHSSEALKIIERHLRTNRQNILS
                     GRFFSSEKEGGSKRAV"
     gene            complement(11648..12388)
                     /locus_tag="PLQ30_03265"
     CDS             complement(11648..12388)
                     /locus_tag="PLQ30_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW01495.1"
                     /translation="MELQGKTAIVTGSARGIGFEIAMVLAREGAFVVLVDQTGTQDAA
                     ISLQEKHYGAMAISLDITDLDAVNNAVKTIVSAKGRIDILVNNAGIIARGSIIDLSRE
                     QWLRVMDVNVNGNFYFCKAILPYMIRQHSGNIVNITSIAGKMGDITAAPVYGASKGAI
                     NAFTKSLARQLAEYGIRVNAVAPHAIETSMSSEWTIEKREKVIDAIPLKRMGKPEEVA
                     EAVLFLASERSSFITGEILNVNGGMLMD"
     gene            complement(12402..13676)
                     /locus_tag="PLQ30_03270"
     CDS             complement(12402..13676)
                     /locus_tag="PLQ30_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPW01496.1"
                     /translation="MLIVGILLLAFLAIGMPIAYAIGISGFAFFLQHPELPMTIIAQL
                     PISQTQNFTLLAVPLFILAGNLMNASGITNRLIRLSTVLTGHMRGGLAQVNVMLSTLM
                     GGVSGSAIADAAMEARILCPAMIERGYSPGFTANCTAWTGLITATIPPGVGIILYGVT
                     GEVSIGRLFAAGIFVGIFLTAVYMFTVWIISGRKGYKAERARASFQECMQAIVENIWA
                     LLFPILLLVGLRIGVFTPSEVGSFACVYAVFVGIFAYKELSLKKLIQTLGESVMDVGG
                     IMFIIALSGIFGYGIPFDRIPDLISNAILGFSTNRYIVLFIILFILIILGMFIDGSVV
                     ILLFTPIFLPLAAQLGWDPVHFGILFCTIITMGNMTPPVGLAMSAVCTVLNVPISEYI
                     KEMWPWFWATVLAVSVLVFFPGLVLALPRLLF"
     gene            complement(13678..14148)
                     /locus_tag="PLQ30_03275"
     CDS             complement(13678..14148)
                     /locus_tag="PLQ30_03275"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HPW01497.1"
                     /translation="MRKIYHFFGKAEVLVSAVCFTVSLLLIFSGAIARTLRHPLNWSQ
                     DLSLFLFAWGVFLSADAAMRADKLVNINLLVARLSENMQKILATVVYVIIFIFLIFLS
                     YNGIKLSIFSHRRVFQGIPGFSYSWVMVSIPVGSVLQMITIILKIRDLYKREEH"
     gene            complement(14226..15224)
                     /locus_tag="PLQ30_03280"
     CDS             complement(14226..15224)
                     /locus_tag="PLQ30_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HPW01498.1"
                     /translation="MKKKSMVLVVFFLIASIFLVSAQTEKKYVLKFGHVLTTQDPYHE
                     AFLKMAEAVSARTNGNLKVEVYPSAQLGVEEDILEQIRQGANIGWNTDAARLGMYVPA
                     IAVINAPYFVDSLEEVKKLNAIPSVSAWKAELERTQGIKILSFMWVQGFRQMITNKPI
                     RKPADLSGLRIRTPPAPIWQESVRAIGAVPVAMAYGEMYSALQTRAIDGLELSFTATA
                     SGKFQEVAKYACETKHILLINFEVVSSKWFNSLPVEYQKILQEECDKAGLEVSRRYLE
                     EIDPKNIQVLKAGGMTIIPASEIDMAAFKAAGEKAYQKLNLVAVRDQVYKELGKTK"
     gene            15298..16218
                     /locus_tag="PLQ30_03285"
     CDS             15298..16218
                     /locus_tag="PLQ30_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HPW01499.1"
                     /translation="MRDVRHFENCALPKSVEVIQGKGGFPLLHILNRYAECSLYLYGA
                     HVVSFKPQDQTELLWLSPYSRFSKGTPIRGGIPICFPWFSKHRTMDYLPLHGFVRTEF
                     WNIESAEDLTDGRTKIELSTESEEPLPDSWPYHFKLKIIITVGEKLEIALIIQNLENL
                     PIICEDGFHTYFKVNNPHGCQVRGLDGIEYIDRTKGDIRERQNGLACFEDEIVHAFMH
                     APQRLELVDLVDNRRICIEQENMDSIVLWNPGKDVGAVNSEIFSAWDQFVCVESTNCL
                     DCQLSIPPLGSHCSILRLSSISFDKEEKRR"
     gene            16235..17326
                     /locus_tag="PLQ30_03290"
     CDS             16235..17326
                     /locus_tag="PLQ30_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPW01500.1"
                     /translation="MSQKIRIDRVRLEQFCTDVFIKLGLPQDEAQDSAEILVAADARG
                     IESHGVGRLWRYKNGLQKGIMKGGILPTTLQETPISLVLDANGAMGLSLSKRTMAKII
                     EKARVAGAAFSSVRNSNHFGIAGFYSEMAAREDMIGIGMTNTAALGVPTFGRKAMFGT
                     NPIAFSIPAHDGRIFTLDMATTVVTRGKIEVYDRENKPLPSGWAVDTRGKGTSDAHRL
                     LEDMLYQRGGGLLPLGGEGELLSGYKGYGLAEVVDILTAILSGGVFGQAVIDSQATSA
                     RVCHFFGALRLDLFRDAEAIKSDMDRLLTDLEMAEPAEGCERVYYAGLKEHEAEAESA
                     HFGVPISEKVALQLRQIAQELGVEPPIIS"
     gene            17506..18162
                     /locus_tag="PLQ30_03295"
     CDS             17506..18162
                     /locus_tag="PLQ30_03295"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPW01501.1"
                     /translation="MIQKDQIDATNKKNMAYEIIKQRIINNEYKPLDTLTEQLICSEL
                     GISKTPVREAFKELEKDGFVKIIPSIGCLVSSIGLDYIHEIFEIREILECAAARIVAE
                     RGDKTIFEEILKNHESFELAEKKNIKGSVVSGFEIHTAIFETVGNSRLLDFYKNIQNH
                     IIRIRLFFIYQFDMEKTERMNAEHKMILKTIIAGNSEAAEKAMREHLRNSQERLKSLL
                     "
     gene            18176..18850
                     /locus_tag="PLQ30_03300"
     CDS             18176..18850
                     /locus_tag="PLQ30_03300"
                     /inference="COORDINATES: protein motif:HMM:NF006731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HPW01502.1"
                     /translation="MIHVLKIAERPDQNIIESFRKLAVATIYEASGKKGFIDCAIKPL
                     AKGMRICGPAFTVQCVPGDNLMLHKALQTAKAGDVLVASMGDGYFYGYLGSLMGTAAI
                     TAKLGGLAIDGCVRDTEELIEMDFPVFCRGACIRGTAKNGLGTINYPLNFGGATIYPG
                     DLIMGDDDGMVVVRRQDLADVLQKSLDRVANEKKKSEQLQRGISSMELNKLEDVCIAL
                     GMKEEE"
     gene            18855..19913
                     /locus_tag="PLQ30_03305"
     CDS             18855..19913
                     /locus_tag="PLQ30_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPW01503.1"
                     /translation="MKIFYRDLQNAACEILRLVGESKENSQIASEIMVINDLRGISTH
                     GTNLLRMIAKRCEAKMLSLPTRVVVVSEDMATLVLDGGDGLGQVAGYKMMNAAIEKAH
                     LYGLGMALLRDTNNVGSLGYYVHKAAMQNMAAIMMTNGNPSMAPYGGAEPFLGSNPIA
                     FAVPTSSEMPVMLDMSSSVVARGKIRMAALKNENIPLDWALDSEGNPTSDPQKALKGC
                     LMPVGGPKGSGLAIIVDIFAGILSGSSYGRNLKSFHELEGKTGVGACFIAIDISRFMA
                     VDKFRNLVGEYARALKSCELREGFEEILLPGEIELRKEQESRENGIDMPVEVINDLNE
                     LLRKAGANFLLRPMDQCR"
     gene            19948..20757
                     /locus_tag="PLQ30_03310"
     CDS             19948..20757
                     /locus_tag="PLQ30_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPW01504.1"
                     /translation="MKMRLGCFGYIKDLDTIAAAGFDCAELHIREIMAMSEGEFKKAK
                     KALRDCGIPAEVFDNPIPLDCCISSPSFYLDYFRDFLEKSADRIAEMGARYIVFGNGK
                     ARSLPSEGDIPIAASKFDDFFISLLEIAAKRNITVLIEPLAKSLSNIVNSLPEAIEFI
                     KKYEKHNLKTLLDYRWMLEDGWPLTDIEDYELYIKHVHLDNPLSPFPTRVVPRMNDGF
                     DYAPFLNTLKRIAYKEIISIEACVFQDFAKEIREGLELFKANNIEPYHSIS"
     gene            20778..21812
                     /locus_tag="PLQ30_03315"
     CDS             20778..21812
                     /locus_tag="PLQ30_03315"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HPW01505.1"
                     /translation="MKALVYYGPHDIRYQDVPTPRPKEGEVLVHVRAVSICGSDLAAY
                     KAVSSMRSPGLIMGHEFSGEIVQLGEGVDNLKVGNRVGVITNIYCGKCTDCLEGRTNV
                     CDHRYIIGTTMKAGSYNGAMSEFVVAPAEKIMLLPDYVSFDEAALVEPLSISLRATKH
                     VAHEVPSRAVAVLGVGPIGLMAIMCMKNLGSAKIVAIDLLQNRLELARKCGATDTILS
                     GQDVPALIRQMTDNVGIDVVFDAAGVPSTVNTAIEIVRNGGSILMVGMASPTLEIAYK
                     HAVSKELKLQASYMYTTEMKEGLQMIADAKLNVAQMITSRHPLSEGPKVFEELASGNT
                     KEVKVILFPE"
     gene            21852..22598
                     /locus_tag="PLQ30_03320"
     CDS             21852..22598
                     /locus_tag="PLQ30_03320"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW01506.1"
                     /translation="MELENTIAIITGSGNGLGMGIAKKMFAEGASLALWDIDFESVKR
                     LAFELDHSGQRVLPLKVDITDEAGVQSAAATTVDKFGHIDVLVNNAGISRHKPLEETT
                     LEIWDAVIKVNLTGTFICCKAVAPVMKKQEFGKIVNIASLGGRTGRPGVGVNYAASKG
                     GVIGLTKLLAKELGPSNIYVNAICPGPILTDQTKQYPREVFDTWNAGRAVPKDGLPED
                     IGDAVVFLASRRSNWITGVSLDVNGGIFIA"
     gene            22637..>22853
                     /locus_tag="PLQ30_03325"
     CDS             22637..>22853
                     /locus_tag="PLQ30_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01507.1"
                     /translation="MSELKVIDINKVEPEKKTPPRSSWILISEKTVGAQNLALGVNET
                     EPGSMVPEHTHETEEEVMFFLEGEGKFI"
BASE COUNT         6469 a         4862 c         5460 g         6062 t
ORIGIN      
        1 tatattagac cttcacctat cgatgcggac atcggcgcac aattggagtt attaagaaaa
       61 gctggcgttg aatcaatatt cgaggaaaaa cctctttcga aagaagcaga caggcccgaa
      121 ttgcgacgtg cgctcgatag tctttgcaat ggcgatacgc tctttgtggc ttctctggat
      181 agacttgctc gcacaattac agagcttttc tatatcctag aagaaataag aagaagggga
      241 gccaatctgg agagcttgaa ggaacaactc aagattgagg atgattttat aaggcatttg
      301 ggcatcatta ttgatttcga gcgcatttct cttttggaat ggcagcgggg aaagatcgaa
      361 gcaaagaaat catggcgaga aagaggggct ggctctaaac gccagcgctg attcagtgtt
      421 aaagatagcc ctattggcgc cgtaccttaa gtttctctga catcaacgac tttttgaaca
      481 acaggagtaa tcgcttcgat caaggttttt tcttcgacgc ctgtcacctt gattgtgcag
      541 taggcattgg ttggatcttc tccttcgaag gtggcaaaag agactatatt accgccgcat
      601 ttcgtaatcg cattggaaac ttttgccagt tctccctttc tctcaggtag aaggagggtt
      661 agcctgatgc ccttgtgtct agcgccaaaa agctcgataa aaagcctgaa tatatccgat
      721 tccgtgataa tgccaacaag tatttcgcca cgcattactg gaataccgga tatctgatta
      781 tcatccatta tgcgtgccgc ttcctcgata ggaaggtctt cggtaatcgt tatgacatct
      841 ctggtcatta ctttatcgac tgtaagtttc gatagcagat agtgcatttc ccatacatcg
      901 agagttgtgg caggcgaagg agatgcatgt gtaaggtcca gagcactgac aataccaaca
      961 agcttgccgt ttttgtcgag aacaggcaat cgtctaatct tctcgcgtcg cataatagct
     1021 tgcgcttcag gaacagtcgt atccggtgta atggtaacgg gatttcgtgt catgcgctga
     1081 cctactttca tattcgcctc cttcgatatt tctcctatta ttatactaca gcgattatac
     1141 atccataaat atattgatag taagtcaaag ccccaaatat gccctgcgaa cttcttgatt
     1201 cttgagaaga tctgctgcgg tatctgtgat tttgataagg ccgttctgca tgacatatcc
     1261 tttatttgcg atcttgagcg ccatatttgc gttctgttct acgagtagga ttgtggtatt
     1321 gcgctctttg ttgatctttt ggataacctc aaatatgttc tggacaataa gcggtgccag
     1381 ccccagcgat ggttcatcga gcaaaagaag gcgcgggttt gccatcaagg cacgcgatat
     1441 ggcgagcatc tgctgttcgc cacccgaaag tgttccaccg agctggtttc gacgctctgc
     1501 aagtcttggg aatattgtaa agacctcttc catgtttcgc tttatgcctt ctctatcgtt
     1561 tcgaagaaag gctcccatat cgaggttctc tgagaccgta agctgcggga aaatgcgacg
     1621 gccttcaggc acctgtgaga ctcccctctg gacgatcttg tggggactca ttctcgaaat
     1681 atcttgacaa tcgagcaaaa tctcgccgga gcgtatggga acgataccgc agatactcat
     1741 cagagtggtt gtcttgcctg ctccattggc tcctataagt gtgactatct cgccttcttc
     1801 tactgagatc gaaacgcctt ttaaggcctg aatatttcca tagaaagtgc ggacatcttt
     1861 aagttcaaga agcgccattt tattctccta gatatgcctt gatgacatcg ggattcttct
     1921 tgatctcgtg cggatttccc tcagcaatga gcctaccata gtccaataca tagatacgct
     1981 cgctaacatc cataacaagg ctcatgtcgt gctcaataag aagaatagtt atatgttcct
     2041 ggtctcggat tatatttatg gtctcctcaa gttcttttgt ctcttgggcg ttcatcccag
     2101 caactggttc gtcaagcaaa agcataaaag gatctgttgc aagagcgcga gcaatctcta
     2161 gtcgccgttg ttcgccataa ggcagattct ttgccgtttc attgacatac gtatcgagtt
     2221 tgagtttctt gagaatggtg tagctctcct cgatgactct ttgctcttct tctcttgtta
     2281 tggcattgcg gattattgct gcaaagactc ctgccttgag cgaattgtgg cggccaatca
     2341 tgacattttc gagaacggtc atattgttga aaaggcggat attttgaaag gttcgggcta
     2401 agcctttgca gttgatgaca ttgggcttta agccttgtat ttcctcgacg cgtccatctc
     2461 gatttcgaat cttgaccttt ccttctgtag gcttgtgtac acctgttatg cagttgaaaa
     2521 tcgtggtctt tcccgcgcca ttggggccta ttagagccgc aatttctcct tcgtcaatgt
     2581 gcatcgatac tccatctatt gcacgcagtc ctccaaaagt catgacgaga ttgtctatct
     2641 caaggatttc attatagtac tgcaacttct ccgtactcat tttgcaagct ccttttctgt
     2701 gaaggtgtat tgcttgcgct tgcgaggaat taggccatca gacttaaaga ttatgacgag
     2761 gatcattgca atgccatata taagcatgcg gtattgcgca aggggcctga aatactcggg
     2821 caggagtttt agaagtattg ccccggcgat tactcccgga atggagccgg ttccgcctat
     2881 gacaacagcc atcaggatca ttatcgattc ccacagagta aaactatctg gattgatata
     2941 ggtggtctgg ctagccatca gcactccggc aattccagcc cagaatgtgc caaccgcaaa
     3001 ggttatcaat ttgttcttga ccatatcgat gcccatcgct tcgcaggcta cctcatcttc
     3061 gcgcatcgct tcgagggccc ttccgacctt tgaatcttcg attcgtctca caacgaatat
     3121 ggtaatcatt acgaagacaa gagctataaa gtatatatac gtggcagcct ctgagggctg
     3181 cagtttttga ccgagaaacc agggacgagg aataaggctt atgccggtag cacctcctgt
     3241 tagatcgccg gcattctgca tcaccatgtg aaagatctca ccaaacgcaa gggtgacaat
     3301 tgcaagataa tcgcccctaa gtctcaatac cggcagactc aatattacac caaagatcgt
     3361 cgctgcaatg cctgcaaggg gaagagatat ccaaaagagc cagcctggat taaagcccgc
     3421 cgcgatgaaa gaaggcccga tatatttcca tataaggcca taagtataag ctccaatgcc
     3481 gaagaacgct gcatagccta gattgagaag ccctccgagc cctacgatga tgttgaggcc
     3541 tagacctaag atcacatata tgaatgcggt gatcatgaca ttggtgtggt acataccaaa
     3601 aagaaaggga aaagccacgg taaatccaaa gactaacaga aggattagcg ggcgaagcgc
     3661 agggttttta aggtatcttc catttagggc acggttattg ataaggccat tctcgatctt
     3721 ttttttgctt gtccgtcttt cattccactc gagagccttg gtccagacga atgagagcac
     3781 gaatgctcca attccaatga gaggaaggac attccagcgg aaattgatat gagccactcc
     3841 ccttgtcaca ttgactttca taaccatgat ggggaaaaat aaaatcatga accaaacggc
     3901 gataatgagt gattttccaa tatctttaag aatagagcgt gctttcatag tctagacctt
     3961 ctgtcgtcga gaacgaccaa gaattccatc gggctttatg agaagtatga ttatgagaat
     4021 aaggaaagca aaggcatctt cataatcgct ggacacatag cccgtaccga agctctctgt
     4081 ccaccccagg acaaaacttc cgagaaccgc tccagggata cttccgatac ctccaagaac
     4141 tgcggcaacg aaagccttta tgccagcaat gaagccgatg tagtagttta tctgtcccat
     4201 ataggagcag atgagtaccc ctccaatggc tgcaagcgac gagccaatga caaaagtgag
     4261 cgaaataact tgatttatat cgacacccac taattgagcc atgtgcatat cctgtgcggt
     4321 ggcgcgcatc gcttttccca tacgggtgaa ttttatcagc aaagtcagca gaaccattat
     4381 tatggctgtg gttatgagta ttatgagttg agtggagttt atgtagcttc tatagggcaa
     4441 tagccagcta aattcaggaa gataattggg aaatggaaga aatttttcgg tctgtgaaag
     4501 aagcacaaag ttttgaagta ttatcgagac gccgatggca ctgatcaaag ccgacaagcg
     4561 cggtttttcc ctgagcggct tgtatgcgat tttctcgatc gtaaagccga atgccgcggc
     4621 atatattatc gaaagcagaa ttgaaaatac aaggacaagg ctgacgggca tgcccttgga
     4681 aaagaagaat ccccctaaaa taagggcggt gaaaccgccg atcatataga tctcaccatg
     4741 ggcaaaattg atgagctgaa taatgccata taccatggtg tagcctaatg ccatgagagc
     4801 ataaatgcta ccttttgctg taccacttaa aaaaagctta ataaaatatt ccatgtaccg
     4861 atcctcgaca gcaggattgg aaaaagaagc cgttccgact cttatgccag aacggcttct
     4921 gaagattcag aagaactcgc cccttacttt aatactataa acttaccgtt cttgacttgg
     4981 aaaagggagt taccatagcc tactatatct cccttcttat cgaatgttat ccggccaagc
     5041 accgtgtcta cgtattctga ctgcaaagct gccacgatct tatcgtaatc cgtggatttt
     5101 gccttttcga tggcattgag cagcgcttgg gctgaagcat aagcatttaa gaagaaggct
     5161 cctggctcgg aaccgaattc tttcttatga gcttcgatcg ccttgttggc cattgggttg
     5221 gcagaggtat cggttgggcc agtagcatag acgccttccg cgtattttcc agccacttcg
     5281 ataaaggtgt tgtcctttat gccgtcttcg gaaataaaag ctacatttat tcccttctct
     5341 ttcatctgct gaacgagttt ggaagcctca gggtgatatc cgccccatac cacagcatcg
     5401 actttggcgt tgccaacttt attgattaca gcggaatagt ccggagcacc ggggttgatg
     5461 ccttcgaaaa gtgctacctt gacgcccatt tcctcagcaa actcccttac agactccgcg
     5521 aatcctttgc catagtcgcc cttatcgtgg agaactgcta ttttctttac tttgagatcc
     5581 tttacaagga agtccgttgc aagccttgct tgtgcatcat ccggggcgat tgtgcggaag
     5641 aagttcggat attcaccact ctgggtaagg aaaggtgttg ttgcggatgg cgagatgcac
     5701 actaatctgg aatccttata aattcccagt gctgctttgg tagctccaga acaaatatgg
     5761 ccgatgaccg cagcgacctt tgtgcttacc agtttggcgg ctacgttggt tgcaatctct
     5821 ggtttgcatt gatcgtcctc gataatgagc tcaacttttt ggccattaat gccacccttg
     5881 tcattgtatt ctttgacaac cagcttggca gcattgacag tgggtagacc ataggaagcc
     5941 aggtccccgg agtgggcgcc ggcgacacca atctttatgg ttggagccgc aaatacagct
     6001 tgagcaccca agaagagcac aaggctcaca atggctgcaa aaagcatttt tctttgcttc
     6061 attttgaatt cctccttccg catatacgaa acatgcgcat aaataatgga atgaagccaa
     6121 tttctgcgaa gaatatatat cccaatactc cgtgtcaagg tgtttctatc aaaagtaaag
     6181 cattttattt ccaaaaggca taatatttta atctttgcat atttcgtttt aattttcgaa
     6241 atacttttcg gcaaaggctt gactaaaagc agcctcagga taatttctca aaaattatcg
     6301 aattttttca ttttatatat tgacgaagtg ccatacagaa gatacaatgc cgaaaatgtt
     6361 actatgcata gtaacataga tttgttgaca tgcaaatgcc gatggctatg gcatggacag
     6421 atcattttgc cttataaggg agaggttgta tgagtaatcg ttttgcaaaa ttgctctatg
     6481 cgattggatg tgcagggatc ttgctattgt tatcttgtgc gccaaagggc gcaaaagttg
     6541 tcggtgtggc taaattcgtg tcgcatccgg ctctcgatgc attagagaaa ggcattttgg
     6601 atgagctctc tgcatcaaag gctgattata aggtcgatct gcagaatgcg aatgcagaca
     6661 tgaatacggc tgcccagatc gcacagcact ttaaacagga gaaggttgca ctcgccgtgg
     6721 gaattgcgac gccaatggct caagcactcg caaatcagat caaggatcgc ccggtcattt
     6781 attctgctgt aacggatcct gttgccgccg atctagtaga aagcatggat aagggcggac
     6841 ccaacgttac cggcacatcc gatatgactc ctgttcggga acagctggat cttctatgtt
     6901 cattgaaacc aagcataaag cgcatcggaa atatctatgc ttcaggagaa gcaaactcag
     6961 ttgctctagc tgagatcgtc agagaatatt gccaagagca tggtttagaa tatgtagagt
     7021 cgactattac gaattcttcc gaggctaggc aggctcttct ctctattgca gggcgaatcg
     7081 atggactata cctgggcaat gacaacacgg tcttctcggc gcttagcgga attgcggaag
     7141 tgggaatcga taaaaagatt ccaatcgtga ctgctgatcc ctcttctgcg gagacaatac
     7201 cggtgctggc ggctctcgga tacgactact atcgcatggg tttggctact ggcaaaattg
     7261 tggtgcgcat attgaatgga gaaaaaacat cggatatccc tgcatttctg tccaaagaga
     7321 atgaagacat gactttcgta ctcaatctcg atactgcaag gaaaatcggc atatcagtcg
     7381 atcagtctat aatcgacagg gcgaaagtag ttatctcaga tgatcaactt gtgcggaagt
     7441 aatcgatggt agaaggaata ctcgtcgaag gattaatcta tggcattctt gcgttcggag
     7501 tttttgtaag cttcagaatt ctggattttc ctgacttgac cgtggaggga tcttttcctg
     7561 ctggagctgc tgcaggcgca gtagtagctt cggctttcaa tctatcctca aatgctgttc
     7621 tgcatgggtt ggcggtaccg gctggtatgc tggcgggttt tgttgtagga gggatagcag
     7681 gttttgccac tgcggctgtt tatcacaagt taagaatccc gcccctgctt gcaggcatag
     7741 ttactatgac gggattttat tcgataaatc tgcgtatcct cggtggaaag ccaaactatc
     7801 cactcataac taacaacccc atgctcaagc ttgcgcgttc atttctcgga ggtgcgcttc
     7861 cctcggagtg gtcgctcttt gcatcatgcg tgctggtatg ccttgtgctc tttgggcttc
     7921 tagatctttt ctttcatacc gagattggca ttgcgatggg tgcgcttgga gacaatgaga
     7981 atgccgtcat tcaggctggc attcagccaa atagattgag aacctgggga ataatgctcg
     8041 ccaatgctct tccaggtctt tccggcgcaa tggcagccgc ttatcagggt tttgccgacg
     8101 tcaatctcgg acagggagtg gtggcttcgg gccttgccac agttatgctt ggcgagctca
     8161 ttatccgttc gcaattcata agcgttcagc ttctcagggt tctcattgga tcgatcttgt
     8221 ttagagcatt gatgtacgcg gctcgctcat ggggctatgt tgccggtatt acccccaatg
     8281 atctaaggct tttgaccgcg cttcttataa tactatccgt ggctctttct cgaatcaaaa
     8341 ggaagcgctc atgaaaaaaa gttgggaaga aggatgatct tatggtacgc atagaagata
     8401 tcgtcgtaag ctttgatttg ggaggtgggc aagaacacac tgttctaaat aagctttcac
     8461 ttgaggtaaa gcaaggtcag acagtgtcga tcatcggttc gaatggggct ggcaagagca
     8521 cgctcctcaa ttctatcgcg gggagtattc cattgcatgc tggacatatt catctaaatg
     8581 gcgaggatgt aacctcctgg gcagaatggg agcgcgccag gtatttgggt cgagtgcgcc
     8641 aagatcctat ggcagggact gctggcgaaa tgacggtaat cgacaatcta gctctcgcag
     8701 ctcgcaaagg gcctcgccga tttcgcattg cgacacctcc gcgatttgcc cgagaaatgg
     8761 agagaaagct cgcagagcta ggcatgggtc tcgaaagccg ccttcgggaa aatgtttcgc
     8821 gactgtcggg cggtcagcga caagcgctaa cgcttctaat ggccgttatc tctcatccga
     8881 cgatcttgtt gctggatgag catacggctg cactcgaccc ggcgaatgcg gagattgtgg
     8941 atgcccttac gcgccgattt atcaatgaat atgacttaac tgctctcata atcactcacg
     9001 atatgaagcg cgcattggag caagctagcc gagtgataat gatgcatgaa ggtgagatta
     9061 tagccgatct aagcggtcca gaaaaggaaa aaatgaatgt tgccgcgctt gttcgcttat
     9121 ttaaaaaagt gcgtggatcg gagtatgcgg aggataggga tctgctgatc taggtctctt
     9181 ttgggattca tgccggacag ccgaaacact gtacattgtc gctggcagtt cgcaccagct
     9241 attttgcact ggcgacctcc aattgcattg attttccgat tgccgattcc catttagttc
     9301 tggaaattaa gataatagcg ataatcgcgc ttgcatagtt cgagaacaag tttccccaga
     9361 agaccgcata atactggaga tacttttctg tcacgagaat gaaagcatag cgcaataacc
     9421 agattcgcag aattcccaaa accagcggga ctctggttcg gccaagccct atgaatgccc
     9481 cctggatcgt agtggttatt ccaaagccta ttacggaata agtgtagata tgcagagccc
     9541 tgttcgcaat ttcgaggaca tcttgtcttc ttgtaaatag tacagtcagc ctcgatgaga
     9601 gagggactat tatggcgata agtatggcgg ccgttaaggc gctgagagta gtgccatgga
     9661 ccatagattc cctggccttt cttggattct ccgcgccgat attcatgctg accatggtcg
     9721 taactgccga accaaatgcg ctgggaacat tgaaggcaac ttgcgttatg ttgttggcta
     9781 tgccttgtcc attgagaacc acaggtccat atttttccac ttcggtgttg attaagaaaa
     9841 aaccgagatt gagaataaaa gtatttgcca tggccggtgc gcctatgcga aaaagctgtt
     9901 ttgcggcgac cggatcgaat ttgaatcctt tgagctccag tctatcgggc ccctttttta
     9961 gaaatagctc ccagaacatc catagagtga taatgatatt cgcagaaagc gaagagagaa
    10021 cgcaacccac aatacccata cgaaattcat agatatagag gaagttgaag gcaatcttga
    10081 gtatgagcat gagtaccatt cttatgaatg gcgcttctgg catgccattt gcgttcttga
    10141 taccgttgta aatagactcc ataaaggaaa atggaagcac aagagcatag agcgaaagat
    10201 atagaaaaac gttgtgagaa atacctggat tgacgcgcga cgaaataatc ttagccgcca
    10261 ggactagaaa aggtgcagaa atacaaccaa gcatcgatcc cataaccaga atctgcgtcg
    10321 aagtacgctt tgattccttg aggtcaccac gtccattgag ctgcccaatg atggccattg
    10381 cggcaacact caatccctgt gccaaagccg tcgtcatatt aataactggc tcgctgaagg
    10441 tgacagaact cgctacaaga gtacccgcga tattgttgat aaacagtcca tccatgagag
    10501 gcatgagtgc ctgaacgaca ccaagcatga gcgtaggagc tgcaagaagc atcaaagtag
    10561 aagaaagtgg gccatttaga atatagtcgc gccgcaactc agtcgactga tgcaagaagg
    10621 ccagtttcat agattatgaa atatttctat tctctttttg aaatctaatc aagatattct
    10681 acgatgggtc gacgataatg cagcctagca tgtgggatat atcaatcaaa tgaataaagt
    10741 cagcaacaca aatgataatt ttgatatcag tgggctaata caatagcggc atgtccaagt
    10801 cgatgcaagt gccggcgcga taaggcataa ttaaaaagat aatagatatc agattctgat
    10861 attattgatt tcaagcgatt atgggtgtat tctagattgg ccaagagaga tcaccgagaa
    10921 aggaatcgaa aatggaagaa tccgacaata tcatcatcga aaagcggagc ctggctgagc
    10981 aagtctatct ttacctatgc aattcgatca gcaatggaaa gatgaagtat ggcgaaacta
    11041 tcaatactag acgcttagcc gaagagctga atatcagtat gatgcccgtt agggaagcgc
    11101 tcaaaaggct ggaaactgat ggcattgtgg agataaaacc tcgctctatg tgccttctta
    11161 gaacacccac aagagagacc atactggatg caattgcggc tcgagagatg ctcgagatct
    11221 atagtgtaaa gtcaatatat gcctctatcg aaaattctcg tcttgatgtg ctgagaaaaa
    11281 tcatagaaga gatgaaaatg gctatatccg aagatccgat cgaccttaga aaatacataa
    11341 agcacgattg gcaatttcat aaagagttat gcaatcttgt tgataatcag tttgtgaata
    11401 agttctataa ggaactgaat atacatctca atatgagtta tgtatacgat ataggtatag
    11461 aaccaaatgt ctctcagact tttagggacc atatagatct ggtgaatgcc ttggctgctc
    11521 actcttcgga agcccttaag atcatcgaaa gacacttgcg cacaaacagg cagaatattc
    11581 taagcggccg attcttttcc tcggaaaagg aagggggtag caagcgagca gtatagattt
    11641 ggaaatctta atccatcagc attccaccat ttacattcag tatttcaccg gtgataaaag
    11701 aagatctttc tgaagcaagg aagagaacag cctccgctac ctcttcgggt ttgcccattc
    11761 tctttaaggg aattgcatct atgacttttt cccgcttttc tattgtccat tctgagctca
    11821 tgctggtctc tatagcatgg ggagccacgg cattgaccct gatgccatat tccgcgagct
    11881 ggcgggcaag cgattttgta aaggcattga ttgctccctt cgaagctcca tagacagggg
    11941 cagctgttat atcgcccatt tttcccgcaa ttgaagttat attgacaatg ttccctgagt
    12001 gttggcgaat catatatgga aggattgcct tgcaaaaata gaagttgcca ttgacattca
    12061 catccataac tcgcagccac tgttctcggc tcagatcgat gatactacct cgcgctatta
    12121 taccggcatt attgacaaga atatcgatcc gaccttttgc gcttactatt gtctttacag
    12181 cattgttgac tgcgtctaaa tccgttatgt cgagcgagat agccattgcg ccatagtgct
    12241 tttcttgcag agatattgcc gcatcttgtg tgccggtctg atcgactaac actacaaagg
    12301 ctccttctcg ggcaagaacc atggcgatct caaatccaat tcctcttgct gaacctgtaa
    12361 ctatagcagt ttttccttgt aattccatat tattctctca ctcagaaaag cagtcttggc
    12421 aaagcaagta cgaggccagg gaagaacacc aaaaccgaga cagcaagcac ggttgcccaa
    12481 aaccatggcc acatttcttt tatatattcc gatataggca catttagcac agtacatact
    12541 gcactcatgg ccaaaccaac tggtggagtc atattcccca tggtaatgat ggtacagaag
    12601 agaattccaa aatgaactgg atcccaccct agctgagccg ccagaggaag gaagattggt
    12661 gtaaagagca aaataactac tgacccatcg atgaacattc caagaataat gagtatgaag
    12721 agtattatga acaatacgat atagcgattg gtcgaaaaac cgagtattgc attggaaata
    12781 aggtcgggga ttctatcaaa aggaattccg taaccaaaga tgccagagag cgcgatgata
    12841 aacatgatgc ccccaacatc cattacactt tcccccaatg tttgtattag cttctttagg
    12901 ctgagttctt tataggcaaa gatgcctacg aagactgcat atacgcaggc aaatgaaccc
    12961 acttctgagg gggtaaatac cccgattctc aaacctacaa ggagaagaat agggaatagc
    13021 aatgcccaga tattctctac aatggcttgc atgcattctt gaaatgacgc ccgagcgcgt
    13081 tcagccttgt aacctttgcg cccagaaatg atccatacgg tgaacatata taccgccgtg
    13141 agaaagattc ccacaaatat ccctgcagca aaaagtcttc caatagatac ttctcctgtt
    13201 acaccatata gaataatacc tactccaggt ggaattgttg cggttataag cccagtccaa
    13261 gcagtacagt ttgccgtgaa gccaggcgaa taacctcttt ctatcatagc ggggcagaga
    13321 attcgcgctt ccattgccgc atccgctatg gctgaacctg acactcctcc catgagagtc
    13381 gaaagcatta cattgacctg agcaaggcca ccccgcatat gacccgtcaa gaccgtagag
    13441 agccttatta gcctattcgt tataccagaa gcattcataa ggttgccagc aaggataaat
    13501 aggggcactg ccagcaatgt aaagttttgt gtctgagata tcggtaactg cgcaataatg
    13561 gtcatgggca gctccggatg ttgaagaaaa aatgcgaaac cagagatacc aattgcataa
    13621 gcgatgggca tcccaatagc aagaaaggct aatagcaaga ttccaacgat taacatatca
    13681 gtgttcttcc ctcttataga gatcccttat cttgagaatg atggtgatca tctgcaacac
    13741 agatcctaca ggaatgctga ccatgaccca cgaataacta aagcccggaa taccctggaa
    13801 tactcttcga tgagaaaaaa tactaagctt tatgccgttg taagataaaa aaatcaaaaa
    13861 aatgaagatt atgacataga cgactgtggc gagaatcttt tgcatatttt cggataagcg
    13921 agctacgagc aaattaatgt ttacaagctt atcagccctc attgcggcat ctgcactaag
    13981 aaagacgccc catgcgaata gaaagagcga tagatcctgc gaccagttta gagggtgtct
    14041 gagggtcctg gcgatagcgc cagagaatat gagcaataga gaaacagtga agcagacggc
    14101 ggaaaccagg acctctgcct ttccgaaaaa atgatagatt tttctcatgc aaacggctcc
    14161 tcttatggat atggaagaga gaagtaaagg cggcgctacc gaggaggtac gccgcccgaa
    14221 agatattact ttgtttttcc gagttcctta tagacctgat ctcgcaccgc gaccaagttg
    14281 agtttttgat aagctttctc gcctgcggct ttgaaggcag ccatgtcaat ttcgcttgca
    14341 ggaataatgg tcatgccacc tgctttaagc acttggatat tcttcggatc gatttcttcg
    14401 agatagcgtc gagagacttc caaccccgcc ttatcgcact cctcttggag gattttctga
    14461 tattctacag gtagggagtt gaaccacttg ctggatacca cctcgaaatt gatgaggaga
    14521 atgtgcttgg tttcgcatgc atacttggct acttcctgga atttgccgct tgctgtcgca
    14581 gtaaaggata attcaaggcc atcgattgct cttgtctgga gcgctgaata catctcccca
    14641 taagccattg caaccggaac ggctccaata gctcgaacgg attcctgcca gatcggcgcg
    14701 ggaggagtcc ttatccgtaa accagaaaga tctgccggtt tgcgaatagg cttattggtt
    14761 atcatctgtc taaaaccctg gacccacatg aacgagagga tcttaattcc ttgagtacgc
    14821 tcgagttcag ccttccatgc agatactgaa gggattgcat tgagtttctt gacttcttcg
    14881 agactatcga caaagtaggg agcgttgata accgcgattg caggcacata catgccgagt
    14941 cttgccgcgt cggtgttcca tcctatattc gcaccttgtc tgatctgctc cagaatatcc
    15001 tcctccacac ccagctgtgc gcttggatat acctcgacct tcagattgcc atttgtcctt
    15061 gctgatactg cttcagccat tttcaagaat gcctcatggt aaggatcctg tgtcgttaga
    15121 acgtgcccaa atttaagaac gtattttttc tctgtttggg ctgagaccag aaaaatagaa
    15181 gcgattagga aaaagacaac taagaccatc gacttctttt tcacttcaag cctccttgaa
    15241 ccttgctaat atctaagata gatgatatca tcattcaaga ggttgtcaag ataaaaaatg
    15301 agagatgtcc ggcattttga aaactgtgct ctaccaaaaa gcgtagaggt cattcaaggg
    15361 aaaggagggt ttcctcttct ccatattctt aatagatatg cggaatgttc tctttatttg
    15421 tatggagctc atgttgtgag tttcaagcct caagaccaga cagaattatt atggctgagc
    15481 ccttatagcc gctttagcaa aggaacacct atccgtggag ggattccaat ctgttttcct
    15541 tggttcagta agcacagaac aatggattat cttcctctac atggttttgt tagaacagag
    15601 ttctggaata tcgaatcagc agaggatctg accgatggac ggacgaagat tgagcttagt
    15661 acagaaagcg aagaaccgct tccggattca tggccttatc attttaaact taagatcata
    15721 attactgttg gagaaaagct cgagatagcg cttataatac aaaacctcga aaatctacca
    15781 atcatctgcg aagatggatt tcatacatat tttaaggtaa ataatcctca tggctgtcag
    15841 gtcagaggat tggatgggat agaatacata gacaggacaa aaggagatat ccgagaaaga
    15901 cagaatggcc tggcctgctt tgaagacgag attgttcatg ctttcatgca tgctccgcag
    15961 cgcttggaac ttgtagatct tgttgataat cgaagaatat gcattgaaca agaaaatatg
    16021 gattcaatag tgctatggaa tccaggtaaa gatgtaggcg cggtaaattc cgagatcttt
    16081 agcgcatggg accagtttgt atgtgtcgag tccacaaact gcctggattg tcagttgagc
    16141 attcctccat taggatcaca ttgcagtata ctgagattga gttccatctc atttgataaa
    16201 gaagagaaaa ggcgctagat aaaggaggcg cttgatgagc cagaaaataa ggatagatcg
    16261 agtcaggctc gaacagtttt gtactgatgt atttattaag cttggacttc cgcaggatga
    16321 ggctcaagat tcagccgaga ttctggttgc cgccgatgcg cgtggtatag agagccatgg
    16381 tgtagggcgc ttatggagat ataagaatgg cctacaaaag ggcattatga aaggcgggat
    16441 tctgccgacg actctccagg agacgcccat ttcgctagtt ctcgatgcaa atggggctat
    16501 gggtctttct ctaagcaaga ggacgatggc taagatcatc gaaaaagcaa gggtggccgg
    16561 ggctgccttt tcttcggtgc gcaactccaa tcactttgga attgcaggct tctacagcga
    16621 gatggccgcc cgagaagata tgataggcat tggcatgact aatacggcag cactcggtgt
    16681 acctacattt ggtcgcaagg ccatgttcgg cacgaatcca attgcatttt caatacctgc
    16741 tcatgatggg cgaatattca cactcgatat ggcaacgacc gttgttacaa gaggaaaaat
    16801 cgaagtctac gatagagaga ataagccttt gcccagcggt tgggctgtgg acacgagagg
    16861 aaaaggcact tcggatgcac accgtcttct ggaagacatg ctttatcagc ggggaggagg
    16921 gcttcttccg cttggaggag aaggcgagct attgagcggg tacaagggtt atggactagc
    16981 agaggtagtg gatattctga ccgcgatatt gagcggcggt gtttttggtc aagctgtcat
    17041 cgatagtcag gctacgtcag cgagggtttg tcacttcttt ggtgcgctgc gactcgatct
    17101 tttccgcgat gcagaagcta tcaaatccga tatggatcgc ttactgaccg atctcgaaat
    17161 ggccgagcct gccgaaggct gcgagcgagt ctactatgcg gggcttaagg aacatgaggc
    17221 agaagcagaa agcgcccatt ttggtgttcc gatcagcgaa aaggttgctt tgcagctcag
    17281 gcagattgcg caggaactgg gagtagagcc accgataatc tcttaaaata aactgctagt
    17341 tcaatgtacc gcttatactg agatttctct agaaaagctc attttatcag attctcgaaa
    17401 tatcaacaaa tttaatattg acagaacgaa aaaccggact tatgctaaaa gtatcttacc
    17461 gatatatcgc tggcatacct ttagaaaagg ggaaagttcg tgcaaatgat acagaaagat
    17521 caaatagatg caacaaacaa aaaaaacatg gcttatgaga ttatcaagca acgcataatc
    17581 aataatgaat ataagccact ggataccctg actgagcaat tgatctgcag cgagcttgga
    17641 ataagcaaga ctccggtgcg cgaggccttc aaggaactgg aaaaagatgg atttgtcaaa
    17701 ataataccaa gcataggatg tctggtttct agcataggac tggattatat acatgaaatc
    17761 tttgaaattc gagaaatcct agaatgtgct gcggctcgca ttgttgccga acgaggagac
    17821 aagacgatat ttgaggagat actcaagaat catgaatcat tcgaacttgc ggaaaagaag
    17881 aatattaaag gctccgtagt ctctggattc gaaattcata ctgccatttt tgagactgtc
    17941 ggaaattctc gacttctaga tttctataag aatatccaga atcacataat acgtatacgc
    18001 ctctttttca tctatcaatt cgacatggaa aaaacagaga gaatgaatgc tgagcataag
    18061 atgatcttga aaacgattat tgcaggcaat tccgaagctg ctgaaaaggc gatgagagaa
    18121 cacttaagaa atagtcagga acgccttaaa agtctgctat aggaaggagg acaacatgat
    18181 tcatgttcta aagattgccg aaagaccaga ccaaaatatc atagaatcat ttagaaaatt
    18241 agcagttgca acaatctatg aagcttctgg caagaaggga ttcatagatt gtgctataaa
    18301 accgcttgct aaaggaatga gaatctgtgg ccctgctttt acggtacagt gtgttcctgg
    18361 agataatctt atgctgcata aggcgttaca gacagcaaaa gctggtgatg tgttggttgc
    18421 aagcatggga gatgggtatt tttatggtta tcttggttcg ctgatgggga cagcagccat
    18481 aacggcaaaa ctagggggcc tcgccataga tggttgtgtc cgcgatacag aagagcttat
    18541 tgagatggac tttcccgtct tctgtcgcgg agcatgcatt agaggaacgg ctaaaaatgg
    18601 cctgggaaca atcaattatc ctctcaattt tggtggtgca acaatttatc ctggcgatct
    18661 aattatgggc gatgatgatg gaatggtggt ggttcgacgt caggatctag ctgatgtcct
    18721 tcagaagtcg cttgatagag tcgcaaatga aaagaagaaa tcagagcagc ttcagcgcgg
    18781 tatttcgagc atggaactaa ataagctcga agatgtctgt atcgcactcg gcatgaaaga
    18841 ggaagagtga ggatatgaag atattctatc gggatctaca gaacgcggcg tgcgaaattc
    18901 taaggcttgt cggagaaagt aaagagaatt ctcaaatagc ctctgagatt atggttatca
    18961 atgatctgcg gggaataagt acacacggta caaatctcct tcgaatgata gcaaaaagat
    19021 gcgaagcaaa aatgctcagt cttcccacaa gagttgtcgt tgtttccgaa gatatggcga
    19081 ctctagtatt ggatggagga gatgggcttg gccaggttgc aggctacaaa atgatgaatg
    19141 ccgctataga aaaagcacat ctgtatggtc ttggtatggc gctcttgaga gataccaata
    19201 atgtaggctc acttggttat tatgtgcaca aggctgccat gcaaaatatg gcggccatca
    19261 tgatgacaaa tggaaatcct tccatggcgc cgtatggagg ggctgagcct tttcttgggt
    19321 ccaatccaat tgcttttgcg gtacctactt cttccgaaat gcccgtgatg ctggatatgt
    19381 cgtcgtcagt tgtggcaagg ggcaagatca gaatggctgc tcttaaaaat gagaatattc
    19441 ctctagattg ggctctggat agcgaaggta atccaaccag tgatccacaa aaagcgctta
    19501 aaggatgtct tatgcctgtg ggtggcccaa aaggttctgg attggcaata attgtggata
    19561 tttttgctgg catactctcg ggctcatctt atggacgaaa tctgaagagc ttccatgaac
    19621 tggaaggcaa aacaggagtc ggagcttgct tcatagctat cgatatttcg cgctttatgg
    19681 ccgtggataa attcaggaat ttagtcggag agtatgccag agccttgaaa tcttgtgaac
    19741 ttcgtgaggg tttcgaagaa atactcttac caggagaaat cgagctcaga aaagaacaag
    19801 aatcaagaga aaacggtatc gatatgccgg tagaagtgat aaatgaccta aatgagctat
    19861 tacgaaaagc aggagctaat tttctgctga gaccgatgga ccaatgtcga tgaaagatgg
    19921 aaaggggcat ttgataagga atcgaagatg aagatgcggc ttggctgttt tggatatatc
    19981 aaagatctgg acacgattgc ggcggctggt tttgattgcg cagagctgca cattagagag
    20041 atcatggcca tgagcgaagg cgaattcaaa aaagctaaga aagcgctgag agattgtgga
    20101 attcctgctg aagtattcga taatcctatt cctctcgatt gctgcatatc atcaccttcg
    20161 ttttatttgg attattttcg agattttctt gaaaaaagcg cggatcgaat tgctgaaatg
    20221 ggagcgcgct atattgtctt tggaaatggc aaagctcgaa gccttccatc agaaggagat
    20281 attccgatag cggccagtaa attcgatgac ttttttataa gtcttctcga aatagcggca
    20341 aagcggaata tcacggtgct tatagagccg ctggctaaga gtctatcgaa tattgtgaat
    20401 tcgctccctg aggcaattga attcataaag aaatatgaaa agcacaatct caagactctt
    20461 ttagactatc ggtggatgtt agaagatggt tggcctctca cagatatcga agactatgag
    20521 ctctatatca aacatgtgca tctagacaat cctctgtcgc catttcccac aagggttgtg
    20581 ccgcggatga atgatggttt tgattatgct ccttttctaa acacactcaa aagaattgct
    20641 tataaagaaa ttatttccat cgaggcatgt gtatttcagg actttgctaa ggagatcagg
    20701 gaaggcttag aattatttaa ggctaataat atcgagccat atcacagcat ttcttgattc
    20761 caataaggag gcattctatg aaggcattgg tatattatgg acctcatgat attcgatatc
    20821 aagatgttcc aacaccaagg ccaaaagaag gtgaagtcct ggtacatgta agagctgtct
    20881 ctatttgtgg ctcagatctt gcggcctata aggctgtttc ctctatgagg tcaccgggtc
    20941 tgataatggg gcatgagttc tccggtgaga tagttcaact gggagaagga gtagataatc
    21001 tcaaggttgg gaatcgtgtc ggtgttatca cgaacatcta ttgcggaaag tgcacggatt
    21061 gcttagaagg cagaacaaat gtgtgtgatc accgttatat catcggtaca acaatgaaag
    21121 ccggaagtta caacggtgcg atgtccgaat ttgtcgtagc tccagcagag aaaataatgc
    21181 tcttgccaga ttatgtctca ttcgacgaag ccgctcttgt tgagcctttg tccatatcgc
    21241 ttcgagcgac caagcacgtg gcccatgaag taccgagcag ggcagtagcg gtattgggcg
    21301 tgggacccat tggtctaatg gctattatgt gcatgaagaa tcttggaagc gcaaaaatag
    21361 tcgcaatcga tcttcttcaa aatcggcttg aacttgcccg aaaatgtggt gctaccgata
    21421 caatactctc gggccaagat gtaccagccc tcattcggca gatgacagac aatgtgggga
    21481 tcgatgttgt ctttgatgcc gcaggggttc cttcgacagt aaatactgcc atcgaaatcg
    21541 taagaaatgg agggagcatc cttatggttg gcatggcatc gcccaccctg gaaattgcgt
    21601 ataagcatgc ggtaagcaag gagctaaaac tgcaggccag ctatatgtat acaaccgaaa
    21661 tgaaagaagg cttgcagatg attgcagatg ccaaactcaa cgtagctcag atgatcactt
    21721 cgcggcaccc gctttctgaa gggccaaagg tgttcgagga gctcgcttcg ggcaatacca
    21781 aagaagtaaa agtcattttg tttcccgaat aaaagtaaga tatatgcaga tagactgaga
    21841 ggctagagaa aatggaactt gaaaatacta tagcaatcat tacaggctca ggtaatggtc
    21901 ttggcatggg catagcaaag aagatgtttg cggaaggcgc aagtctggcc ttgtgggaca
    21961 tcgactttga atctgtaaag agattagctt ttgaactgga tcatagcgga cagagagtac
    22021 ttcccctaaa agtggatatc accgacgaag caggggtgca aagtgcggcc gctactacag
    22081 ttgataaatt cggccatatc gatgtattgg tcaacaatgc tggtatttcc cgtcataagc
    22141 ctcttgaaga gaccacattg gaaatttggg atgcggttat caaagtgaac ctcacgggaa
    22201 catttatatg ctgcaaggct gtggctcctg tcatgaaaaa gcaagaattc ggaaaaatcg
    22261 tcaatattgc ttcgctggga ggcagaacag gtcgaccagg cgtcggtgtc aactatgctg
    22321 cttctaaagg aggagtgatt gggcttacaa agctgcttgc aaaagaactc ggaccgtcga
    22381 atatctatgt caatgctatc tgtcctggtc caatattaac agatcagaca aagcaatatc
    22441 caagagaggt attcgacacc tggaatgcgg ggagggcagt ccctaaagat ggattgccag
    22501 aagatattgg cgatgcggtg gtattcctgg catccaggcg ttcgaattgg attacaggtg
    22561 tgtcactgga tgtcaatgga ggcatcttta ttgcgtaaca aataggctta gaagaataaa
    22621 gcagaaggag gaaatgatga gtgaacttaa ggtaattgat atcaataaag tggagccaga
    22681 aaaaaaaacc cctccgcgct catcttggat acttatttca gagaagactg ttggggctca
    22741 aaatcttgcc cttggtgtca atgaaactga gcctggctcg atggtccccg aacatacaca
    22801 tgagactgag gaagaggtta tgttttttct ggaaggcgaa ggcaaattca taa
//
LOCUS       DAOMZL010000023        22559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_1971_length_22559_cov_13.910949, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000023 DAOMZL010000000
VERSION     DAOMZL010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 22559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22559
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1971_length_22559_cov_13.910949"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1569
                     /locus_tag="PLQ30_03330"
     CDS             <1..1569
                     /locus_tag="PLQ30_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HPW01508.1"
                     /translation="IDLKREIDRVASWGEVSMTMLERVVEGAGFSLRFFDFPAKELIA
                     LPKETEYIRISAPKGRCRISLIVREGDQIPDLPPSFLEFLPPEIPLSQMRQEIALIEN
                     QKQEIIAKLRKHSANLSLIKKYLHMLDYNIILERLRSGMPQHESFAYMKGYVPVRDCE
                     KFKKTAAKYGWAIAFDDPVEEDQPPTLIENSPAVRIIQPVFEFLGTVPNYWEYDISSW
                     FLIFFSIFFAMIFGDASYGAIIVLATIASIYAGKRKGKPIVDAQKLFLLLGSATIIWG
                     ALTATWFGINFDNLPKLLQNISLPLINGQSPDSENNVKVICFLIGLIQLSIAHIKNFK
                     RDFPDLKCMSQAGSLLLLLGMFNAALNLVIDATRYPIENWAIGCIAIGFFLVFIFGNW
                     NGKLGSSLIESLKNIIPTFLGTVSVFADIVSYIRLWAVGLAGLAFSQTVNGMASGMLG
                     GPSGFLIGFVLKLIIAAVILVAAHALNFMLTVLSVIVHGIRLNMLEFSGHLGMEWSGY
                     KYDPLKEDENLSAS"
     gene            1596..2018
                     /locus_tag="PLQ30_03335"
     CDS             1596..2018
                     /locus_tag="PLQ30_03335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525271.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HPW01509.1"
                     /translation="MNIALIGAACVLGFGAIGSGIGAGIAGMAAIGSWKRSYLNNKAA
                     SFLLVAFAGAPLTQTIYSFILMSRIINSTKDPLLLLASGVTAGIAEAVSAIAQGKAAA
                     AGCDAFGETGKGFANYIIVVGLCETVALFVLAFTFSAI"
     gene            2061..3146
                     /gene="ispG"
                     /locus_tag="PLQ30_03340"
                     /gene_synonym="gcpE"
     CDS             2061..3146
                     /gene="ispG"
                     /locus_tag="PLQ30_03340"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPW01510.1"
                     /translation="MKETLTVKVGDVLLGGEWPVRIQTMWKNPLPIFSSAEEAQLLPV
                     LEQLSCLKNLGCDIIRFAVPEISTAESLGILAGLSPMPIVADIHFDWRIALRCMDFPI
                     AKIRINPGNIGADWKVREVATKAKDKAIPIRIGINAGSLPRDLAREKDVIQAAIKAAD
                     REIELLESMSFHDIVVSLKMNNPDEVVRANEEFSLHYPYPLHLGVTEAGPLVAGIVRN
                     TAALVPLLKKGIGATIRVSLSDAMEQEVIAGKEILACAGKGKHGVRIISCPRCGRASF
                     DTHAFVSRWMNRLYSINADISIAVMGCIVNGPGEARQADLGITGAGNSVLIFKHGEIL
                     RRVEKEQADITFEEELEALLKDEIEFN"
     gene            complement(3217..3546)
                     /locus_tag="PLQ30_03345"
     CDS             complement(3217..3546)
                     /locus_tag="PLQ30_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPW01511.1"
                     /translation="MNQTGKPCHEEKLRPYAQKFKAIGHPIRLKILCLIAGQEVPCVG
                     DIWRCLDQPQPVVSQHLAILKHYGIVTSEVKKTRRIYSICDPFIRNIIDAMICQINTE
                     PNSTSEK"
     assembly_gap    3635..3734
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3793..4194)
                     /locus_tag="PLQ30_03350"
     CDS             complement(3793..4194)
                     /locus_tag="PLQ30_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01512.1"
                     /translation="MLVLVLIEQTSIADLVLRQLRQKSIASIAYRDPLRLLDHLAELN
                     PEAVIIRGKDFPLHQQLLAALIRYYVPLNHCKMIVLGRGIPAFPPCACIAEEQFLKDP
                     SLLTNDLSKTLRFDSRTGSRLVAKAQRTMKL"
     gene            4275..7670
                     /gene="mfd"
                     /locus_tag="PLQ30_03355"
     CDS             4275..7670
                     /gene="mfd"
                     /locus_tag="PLQ30_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPW01513.1"
                     /translation="MKKDNLQALFEKISRNPALGEIQKLIEQNSFPLHIQNSENSLLA
                     LSFATLVKRTKRRFLVVLPSDHETEEFSQDLALTGVDLLSFPSWFGAPYRAIPLYSRM
                     FTERASALARLAEGDFEIATVSARTLAIPVPPPEYIKNHILPLKIGADFEPTLISSKL
                     AELGYLRVPSVSLPGEFAVRGEVLDIYMPGYEYAVRIHFDYDRIERIIRFDPETQTGR
                     EKLTQIHVRPLKELVWDKTRIYQLQNNATDFMRNDPRFKEIIEILDSEKQMQGEELWF
                     PLAFDKMYNLVDYLDNRAMLVLVGSERIYAISETLRKEYASMYRIALREVLVPPPNKI
                     HISPDGLFAAFSSVLFCHALKGVDGSAERISLEAEPPHSYFGNIRLFKEELSGFKKEN
                     YKTWIIASTELQAGRISSLIQDDSIVIAKGPISAGFTIPSLKLRVLAEHELFGRRKHV
                     PRSIAKTKSAVIESFIDLTPGDYVVHNNYGIGKFAGIERLIVLGLERDYICIEYAGNE
                     KIFVPIEQANLVQRYIGNEGQAPHLDSVGSKVWENRKKRVSKSVEELAERLIRIYARR
                     KNARGFSFPSDNEWQIQFEAAFPYEETEDQLKCIEEVKRDMESSKPMDRLVCGDVGFG
                     KTEIAMRACFKAILAGKQVAFLAPTTILAEQHFENFLERAGDFPIKVALLSRLVDRKT
                     KNAVIKGLADGSIDMVIGTHRILQKDVRFKDLGLLVVDEEQRFGVKDKERLKEMKVGI
                     DCLTLTATPIPRTLHMSLLKIRDMSVLQTAPLERQPIQTFVEEFSPELVARAIRNEIA
                     RGGQIFYLHNRIETLPEVEYFIRQLVPEAIVESAHGKLNGQDLESIMHRFIHGAFHVL
                     VSTTIIENGIDIPNVNTIIIDRSDNYGISQLYQLKGRVGRSDRQAYAYLLYPDKRALS
                     EIAMKRLQIISDFTELGSGFKIAMKDLEVRGAGNLLGREQSGNIYAVGFDLYLKLLDD
                     AVSRLSGIQNEEEEPYLELEYSGYIPDSYISAPMIKMEIYKRIASVWTQGEIDSLHEE
                     LEERFGPLPDEALSLLSLAEMRVLCRKLSIGRLQERAGIVTVEFSKVSKISAEKILRL
                     IRESAGTIRLSTEKPNAIRIQTKAIGLKEKSAYLKERLSLLAD"
     gene            complement(7634..8440)
                     /locus_tag="PLQ30_03360"
     CDS             complement(7634..8440)
                     /locus_tag="PLQ30_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPW01514.1"
                     /translation="MSENDKNRGTLYLLPVPLLPYSPEDWKPCQLMNMIAPNVIAIMK
                     SVPYFVVESEKTANRLLSRLLSPEQFSEVGFFILNEHSKEEDLKAPLSALKSGYDCAI
                     LSEAGLPCIADPGAALVSLAHEEQLRIVPIGGDSSLLLALAASGLNGQRFCFLGYMPI
                     RSEELQKELVLEGQAAMHDKAARIFIETPYRNERTLRACIEALPENLYLCVACNLGTN
                     QPLIRSMPIASWRNKAIIIPEKPAVFCFGIPAKLNKKQLQSARRERRSLR"
     gene            complement(8464..9300)
                     /locus_tag="PLQ30_03365"
     CDS             complement(8464..9300)
                     /locus_tag="PLQ30_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPW01515.1"
                     /translation="MNLSEIYYPRSIQELIAIMARQKNLQIVAGATSFGYIQDTRYLF
                     FDPSIASIRNIPELKTIHKTERMISFGAACALNDLESVYPFEKKPAREVLRATANSTV
                     RNIATIGGHLMYQKRFLALWPLLACLDAELEFKGPSGTSIKNIWYLTNEEGLPSLESR
                     RVLTRIRIPLQSIDRLFIRRTGAEIFPNGDGAYLVAASTIERNSISYFRLVIAGQRAF
                     RDYDAEQRITSSPYPLTERVFHSVLRMYSESLHRTKFWNMELFLPLISQALEDLQRSS
                     VL"
     gene            complement(9297..9776)
                     /locus_tag="PLQ30_03370"
     CDS             complement(9297..9776)
                     /locus_tag="PLQ30_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01516.1"
                     /translation="MNIEFFLNGNLQRIAITSVQRAFDVLVDQCGIKSLSEICPDSEC
                     SNCLILYDGRLVQSYILPAFLLRERHIETIENFTKSKEYGILSAEFDKEGLLSCAYCN
                     NSLMLVAIHLIQRSLSPSWHEISDILASLHCSHIDPRAFADALSKVIQQLPIARKTP"
     gene            complement(9766..11646)
                     /locus_tag="PLQ30_03375"
     CDS             complement(9766..11646)
                     /locus_tag="PLQ30_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPW01517.1"
                     /translation="MTRDEAVPFEQLLAQDMLYAFAIRAFSNIGNIQNINVPPLPEGY
                     TLITADRLPFKNELRIEGMSLPLLAAKKVSYIGEVVGLIVGPDPLLTEELALNTIVDC
                     EAQESRLDWQVFSSSDISARIDYSRGKEKLSSESLKASEAEDSLELASYLRLEPHEFS
                     FHSGLGALAEWENGRLKLSCPALAPELIHSCLAELTGLSTEDIEILQSKMYDNSGIYF
                     WYPSLLAARSAAAARVLGRPIKLLISIQREKRFLPGIHGISLHMKTRWSTQKHCLMDV
                     ACRFAIPVGAYSVLDELILEKTVRLVADAMPNIPLTIIGFTVKTNMIPMGALDCISSS
                     AIFSMLETHIARAARKMDVELLELLWMTFSGIDSSSIMNKMPPKEESSTEKILRPLLE
                     HTDFHRKYAAYEQIHKRSHGAKETRIRAISLSIAEQSHIDSSCISTMADLAKIRNNWI
                     SYKRYGEGLPIKIQIFSSMKQSDVSDSQSKSIRHLSQGAAILEVDYDSIRSIFHSLRL
                     DISIYVGKILCQSAAKGAIRSASIEALLACLSRSIYQDRNIDDIYKWIFSEGRINIEL
                     MKDEKANFERSIGDLAYTLVLSCFLEVMQQLNSSERLILPLKPTPTAMYSSDLLEKDD
                     EH"
     gene            complement(11633..12187)
                     /locus_tag="PLQ30_03380"
     CDS             complement(11633..12187)
                     /locus_tag="PLQ30_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HPW01518.1"
                     /translation="MRFYAIQVITCKEEEWLERIQHQVPDIKFHKIMKKMYIRKKGKA
                     RLDNSPVFPGYVFFEYDGDSLPLEIVYRLRHSKSFIRFLPNNEGPQPLNNRDSEIIRH
                     FISFGSLIPPSLVRFDENQRIKVIEGPLQGIEGFIIKVNRRKRRAKVRLDIADSIILL
                     DLAFEVMEAETAKAAPLGVYHDKR"
     gene            12294..14228
                     /gene="ligA"
                     /locus_tag="PLQ30_03385"
     CDS             12294..14228
                     /gene="ligA"
                     /locus_tag="PLQ30_03385"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPW01519.1"
                     /translation="MPDDLRISEDPDIRQKRIRELEYLIRHHQELYYNGEPEISDEEF
                     DALWDELASIDPANPIIASIGADRSDRWPKYRHRMIMGSLSKASDPESFLAWAAKVRY
                     PLYLVQYKLDGASIELQYDEGGFARAVTRGDGVIGDDITPNAVRMNGVPNRLPEPFTG
                     SVRGEVLMSKRVHASKYSDKANCRNAANGLMKRKDGQGVEDLDIVCYDAVGRLPTGEA
                     FSTEREKIAWLKRMGFNVVPTIECKSPEEIIVYRAKIMELRPTLEYDIDGLVVKGDRI
                     DFEDMNKMRPELQIAFKFSPEEAVTTLIAVEWSESGTTVTPIGIVEPVRLAGTTVQRA
                     NLANPGIIRSMDLRIGSKVVITKRGEIIPKIESLVENPKDAIPIPIPSHCSLCGAGLI
                     DEDTRLYCPNPECPGKAYHRLEKWLSVIDIKDIGSTLLKRLFEAKRVVRIPDLYSLTI
                     DDLISFERIGRKSAQKILRNIRERNEVSLSQFIAGFDIEGIGLLMAEKLIEAGYDTLD
                     KILAASPADFEKIAGFAEISAKTLYEGLRLVEEDMRKLVDSGYVRIRPPLPKTQRTAN
                     PVSGNSFCFTGELRSMKRSEAEKLVREKGGIIKSSVTKDLDYLVTNDPSSGSEKNRKA
                     KELSIKIIDEEQFLEMAGLK"
     gene            14239..14550
                     /locus_tag="PLQ30_03390"
     CDS             14239..14550
                     /locus_tag="PLQ30_03390"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HPW01520.1"
                     /translation="MRTPSARLLEHLDILKPALHEEKEGIVLDVYVIPRAPYDSVEGL
                     RDGRILLRVKAAPEKGKANEAVLDLIADYFDIASSNLRILRGHSSKRKSILLQSFREN
                     R"
     gene            14564..16336
                     /gene="argS"
                     /locus_tag="PLQ30_03395"
     CDS             14564..16336
                     /gene="argS"
                     /locus_tag="PLQ30_03395"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPW01521.1"
                     /translation="MEYFVDQWKSIIANCLNELALQKELKDVSFSSQDIIAEKPPRPK
                     LGDIGFPMFSYAKQFRMSPDRIAAEIASLCNMTIDIEKLILPGTIKATGSYLNIYLDK
                     EYVTNYVLENAEKEGWGSSPLFNGQRIMIEFSCPNTNKPLHLGHLRNNVLGESLSRIM
                     KAAGAEVKKVNLINDRGIHICKSMLAYLAYGQGRTPEDEGLKSDHFVGKYYVLFNRLK
                     EEDPFAEEKAQELLQKWEAGDPDVIALWEKMNKWAVEGIMDTYRRQRVSFDEFHFEHE
                     TYKLGKQEVLEGLARGIFYRAEDGAIQVDLEDVGLGKKVLLRKDGTSIYITQDIGTAI
                     MRYNRWPFHELVYVVASEQQYHFKVLFEVLRRLGYSWAENLYHLSYGLVNLPSGRMKT
                     REGTVVDADDLIDELTELAANEIADKGREEAVGDIEDVAEQVALGALHYFLLQVSPGK
                     DMLYNPEQSLSFTGDTGPYIQYMGARASSILRKHKNGEGNAGQGKIDVSLIVSDSDWS
                     LMRLLMDFPYTIEQSAKNKDPSILAAYAHQLASEFSAWYRDNPVLKNDNPDISASRLA
                     LVTAIKEILRRASNLLCIPFLDAM"
     gene            16456..16767
                     /gene="rplU"
                     /locus_tag="PLQ30_03400"
     CDS             16456..16767
                     /gene="rplU"
                     /locus_tag="PLQ30_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPW01522.1"
                     /translation="MYAIVEINGKQYHAEQGKDLVVDRFDAMPGQSIVFEKVLLLSGD
                     ETKVGTPYVNDAAIKATVREEIKGDKIIVFKYKPKKGYRKTRGHRQPYTVLTVNEIVG
                     A"
     gene            16797..17042
                     /gene="rpmA"
                     /locus_tag="PLQ30_03405"
     CDS             16797..17042
                     /gene="rpmA"
                     /locus_tag="PLQ30_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPW01523.1"
                     /translation="MPHKGVNGRDSNPQCLGVKAYASERISAGSIIVRQRGSKINPGK
                     NVGKGKDDTLFALIDGTVRFHEYKGKKFVDVEPAIQA"
     gene            17126..18232
                     /gene="obgE"
                     /locus_tag="PLQ30_03410"
     CDS             17126..18232
                     /gene="obgE"
                     /locus_tag="PLQ30_03410"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPW01524.1"
                     /translation="MIKFADEAIISVASGKGGDGCVAFRRERFIPLGGPAGGDGGRGG
                     DVVFVVKRNLRTLAYLRYRQVFKAENGQPGMGKNMHGRDGKDVIIEVPPGTILRDAQT
                     NEIIRDFVYRARGKKLGALEDEEPWIFLKGGKGGWGNTHFKSSVNQAPRFAQPGQPGQ
                     ERKLKVELSLIADIGLVGFPNAGKSSLLEYFTNARPKIAPYPFTTKIPNLGVLTVNER
                     DIILADIPGIIEGAHEGAGLGLRFLKHIARTACLAFLVDLSDEHWKAAFPTLLRELEL
                     FSPTLVKKPRILLGTKMDLSESQANFIDFKASFPEESIYGISVFTGQGLDEVKQQLFE
                     KVLAYEAAENYESQKENAKERIFDEIDFSDEGFI"
     gene            18232..18810
                     /gene="nadD"
                     /locus_tag="PLQ30_03415"
     CDS             18232..18810
                     /gene="nadD"
                     /locus_tag="PLQ30_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HPW01525.1"
                     /translation="MRIAIIGGSFNPPHIGHMILIEEILATGRYQRVLLIPAKTAPHK
                     VPQMDPGPEMRLALLEASIEGWPKVAIEDCELKRTGLSYTSDTLEELWDRRLFDERPG
                     LVIGDDLAPDFLATWKRAEKILELADIIVAHRLYVENIPLPYPHDYLDNLVIPISSTL
                     VRGRIHGGGAWRSLVMPSVRTIIENYGLYRNT"
     gene            18788..19402
                     /gene="yqeK"
                     /locus_tag="PLQ30_03420"
     CDS             18788..19402
                     /gene="yqeK"
                     /locus_tag="PLQ30_03420"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HPW01526.1"
                     /translation="MDYIEIPEIASIIEKEIACNLSPKRYAHSRSTSETAVKLAERFG
                     EDRWAAHLAGLSHDMCRGLSFEEQNNLVNKHRACIAFLSERPSLAAILSDREYREKMI
                     HGPAAACSLCYRFQVREESILEAVALHSIADDAMSPLAKIVYIADKLEPLRNRSADAD
                     EKMQTLDLDSLFAYTLASVVRWFSESGKPLCPYTAEIYSRMTKS"
     gene            19399..20589
                     /locus_tag="PLQ30_03425"
     CDS             19399..20589
                     /locus_tag="PLQ30_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HPW01527.1"
                     /translation="MSDSKSKILKRSTLPLLVILIVAAVGIFFVVKLTEKGTVESIIK
                     KDQPMAMLFIFESNKKPVSNQLLIWYPSRRKAAIMDIPSTMGIILKSANKMSSIDTVY
                     DSRNPSKFVKEISDYLKYPIDGWFVYDEASLCQTIDLLEGIQMFIPETVMESDSLKGV
                     SLPGGAVVLDGDKTNQYLLYALATDSYSEEISRKQRLLINMLGRLADESASLNKKSNL
                     RLIASKPKSNFSLATRLSIFEHLAQIDHDMIITQFISGSFRSFEGRRLLFPYYDGELA
                     RDVVSQTAKALGIEEDGASQKTIVTIDILNGCGEKGLANTASILFESYGYKVVSVGNA
                     PSFDYAKTVMYDNDGDKAALQQVANIINCKNFGDASVLPGLQKANITIILGKDFNGRY
                     CIGQ"
     gene            20579..20941
                     /gene="rsfS"
                     /locus_tag="PLQ30_03430"
     CDS             20579..20941
                     /gene="rsfS"
                     /locus_tag="PLQ30_03430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPW01528.1"
                     /translation="MDSKAFAYQSAEILAQHKALNVLVMDVHEIAGWADYFVLATCTS
                     STHMRGLQKHIEDFLKVEKIDLLNKPEVTDDQCWLLMDAQDVIIHIMSAEARDFYDLE
                     SLWFNAPRFAVAFRGNGI"
     gene            complement(21036..21371)
                     /locus_tag="PLQ30_03435"
     CDS             complement(21036..21371)
                     /locus_tag="PLQ30_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01529.1"
                     /translation="MNHKIKMTADTQEEFELITLTNGDSADTIYSLRPTVRFDGAYDD
                     EFDYDSDDDEFDDEEDLEDEDDFEEDEEFEDEEDIDEDFDEDEDIGEDEEFEDEDFED
                     EDFDYDTDE"
     gene            21564..>22559
                     /gene="ispE"
                     /locus_tag="PLQ30_03440"
     CDS             21564..>22559
                     /gene="ispE"
                     /locus_tag="PLQ30_03440"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPW01530.1"
                     /translation="MPTLEIKVFAKINIGLKVFQSRTSGYHDIESIFQNVSLADTIWI
                     STSNKSNKARVTIEGDFGCPIESSSVYKAAIEFDRATDGIIRRNGVHVQVGKEIPAQA
                     GLGAASADAAATLCGLNELFGNRFSKKELARISIRIGSDVPFFLFGEGAAIVRGRGER
                     ISLIKPRTDFGIVILLPAWASSTKDAYRLLDVMRRNGLSRIHSSTEVCEGESTYRVCH
                     SDGDLKKWYDGPIAYWHLGNDFQQVMEKQHREYEVWSQLLIDNGAVCTSLTGSGSAMY
                     GVFESMREADAAAEECRNLIKRAEKSELCNIVSIFSVVPLARSMIVSYIQACNVDT"
BASE COUNT         6322 a         4585 c         5378 g         6174 t
ORIGIN      
        1 attgatctaa aaagagaaat agatagagta gcgagttggg gagaagtctc gatgacaatg
       61 ctcgagcgag tcgtcgaagg agcaggtttc tctcttcgat tcttcgattt tccggctaag
      121 gagctgattg cactgccaaa agagaccgaa tatattagga tttctgctcc aaaaggtagg
      181 tgcaggataa gcctcattgt ccgcgaaggc gatcagatac cagatctgcc cccatcattc
      241 ttagaatttt tgccgcccga aattccgctt tcgcagatgc gacaagaaat tgccctgatc
      301 gaaaaccaaa agcaggagat catagcaaaa cttcgaaagc actcagcaaa tctatctctt
      361 attaagaaat atctgcatat gcttgattac aacataatac tagaacgatt gcgatccggc
      421 atgccgcaac acgagagttt tgcttatatg aagggatatg ttcctgtgcg ggattgcgag
      481 aaattcaaga agacagccgc taaatatggc tgggctattg ccttcgatga tccggtagag
      541 gaagaccagc cccccaccct tatagaaaat tcaccggcag tgagaataat ccagcctgta
      601 ttcgagtttc ttgggacagt gcctaattat tgggagtatg acatatcgag ctggtttttg
      661 atatttttct cgatattctt tgcgatgata tttggagatg ctagctatgg agcgatcatc
      721 gttctagcaa cgattgcttc gatttatgct ggaaaaagaa aaggaaagcc aattgtcgat
      781 gcacagaagt tatttcttct tttgggaagt gcaactatta tctggggcgc gctgactgct
      841 acctggttcg gtataaactt cgacaatctt cctaaattac ttcaaaatat atctcttcct
      901 ctcataaatg gtcagtcgcc ggattctgaa aacaatgtca aggttatttg ctttctgatt
      961 ggccttattc agctttcaat tgctcacata aagaatttta agcgagattt cccagatctg
     1021 aagtgtatgt cgcaagcggg ctcgcttctg ttgcttcttg gcatgttcaa tgcagcgttg
     1081 aatctggtga ttgacgcaac tcgatatcca atagagaatt gggcgattgg ttgtatcgcc
     1141 ataggtttct ttcttgtatt catattcggc aattggaatg gtaagttagg ttctagtctt
     1201 atcgagagct tgaaaaacat aattccgact tttttgggca ccgtaagcgt ctttgccgat
     1261 atcgtttcct atataaggct ttgggctgta ggacttgcgg ggctcgcttt ttctcagacg
     1321 gtcaatggaa tggcctcggg aatgcttggt gggccttcgg gttttcttat tggtttcgta
     1381 ttgaagttga tcattgcagc tgtaatttta gtcgctgccc atgcgctaaa tttcatgctt
     1441 acagttcttt cggttatagt gcacggcata cgactcaata tgttggagtt ttcgggacat
     1501 ctcggcatgg agtggtccgg ttacaaatat gatccgctga aagaggatga gaatctttcc
     1561 gcgtcttagc aatctaccct tgaggagagt ggtgtatgaa tattgcgttg attggtgctg
     1621 cgtgtgtgct tggatttggt gccattggct ccggtattgg ggcaggaatt gctggcatgg
     1681 cagcaattgg atcctggaaa cgctcttatt tgaataataa ggctgcatcg tttttgctcg
     1741 ttgcctttgc aggagctccc ttgacccaga caatatatag cttcatcttg atgagcagaa
     1801 taataaactc cacaaaagat ccgctgctat tgcttgcttc aggagtcact gcaggaatcg
     1861 ctgaagctgt ttcagcaatt gcacagggaa aggcggcggc cgcgggttgt gatgcatttg
     1921 gagagacagg caaaggtttt gctaactaca taattgtggt aggactgtgc gaaaccgtgg
     1981 ctcttttcgt attggctttc acattcagcg caatctaatt cttggatagc attcaaggtg
     2041 tcgagtatca gcttttttat atgaaggaaa cgctgacagt aaaagtaggg gatgtgctct
     2101 tgggcgggga atggccggtg cgtatccaga ccatgtggaa gaatccgctt cccatatttt
     2161 catcggccga agaagcgcag cttttgcccg tccttgagca gctttcttgc cttaagaatc
     2221 tgggatgtga cattatccgt tttgcagtac cggaaatatc cacggcggaa tcgcttggta
     2281 tacttgcagg cctttctccg atgccgatcg tggccgatat tcatttcgat tggcgcatag
     2341 cgctcagatg catggacttc ccaatagcga agatccgcat aaatccaggc aatataggtg
     2401 ccgattggaa agtccgcgag gtcgcaacaa aggccaaaga caaagcaatc cctattcgta
     2461 taggtataaa tgccggttct ttgcctcgag atcttgctcg ggagaaggat gtcattcaag
     2521 cggcaataaa agcggctgat cgcgagattg agttgctcga atccatgagc ttccatgata
     2581 tcgttgtatc cttaaagatg aataacccgg atgaggtcgt gcgagccaat gaagagtttt
     2641 ctttgcacta tccctatcct ctgcatttag gtgtcactga ggccgggcct ctggtagctg
     2701 gaattgtccg caacacagca gcgcttgtac cacttttaaa gaagggcatt ggtgctacta
     2761 tccgcgtctc tctctccgat gccatggaac aggaagtaat tgcaggcaag gaaattcttg
     2821 cctgcgcagg aaaaggcaag catggagtga ggattatttc ctgtccacgc tgcggaaggg
     2881 catctttcga cactcatgcc tttgtttcaa gatggatgaa ccgcctatat tcaataaacg
     2941 cagatatatc gatcgccgtt atgggctgca tagtaaatgg cccgggcgaa gctcgccagg
     3001 cggatctagg tattacggga gcaggtaatt cggtacttat tttcaagcac ggagaaattt
     3061 tgaggcgagt tgaaaaagag caggctgata ttacattcga agaagaactc gaagcactat
     3121 taaaagacga gatcgaattc aactgagctt taatgctatt gcaggctaaa ggcctgttgg
     3181 gcgtactgtt ctcttcgagt tatgcgctaa ttctcgtcat ttttcggagg tagaattcgg
     3241 ttccgtattg atctgacaga tcatcgcatc aattatattc ctgataaatg gatcgcagat
     3301 agagtagatg cgacgcgttt tctttacttc ggaagttacg attccatagt gctttagaat
     3361 ggcaagatgt tgagacacta ctggttgagg ctgatcgaga cagcgccaga tatcaccaac
     3421 acaaggcact tcttgaccgg caatgaggca aagaattttc aaacgaattg gatgtccaat
     3481 tgccttgaac ttctgtgcat aaggccttag tttttcttca tggcacggtt ttcctgtctg
     3541 gttcataata tttgtattat atagcaatta tcataaaaac tcaagttaaa aagctgataa
     3601 tgcgatatgc atgccgaaca gccgaacagc cgaannnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnngccgaa cagccgaaca gccgaataga tcaggccgac tttctggctt
     3781 ctcagattat attcaaagtt tcatagtacg ctgtgctttt gcaactagcc tgcttccagt
     3841 cctggaatcg aaacggaggg tttttgatag atcatttgta agtaaagaag gatcctttag
     3901 aaattgttct tcagcgatac aggcgcatgg gggaaaagcg ggaatgcccc taccaaggac
     3961 aatcatcttg caatgattca acggtacata ataacggatt aaggccgcaa gcagctgctg
     4021 atgcaaggga aaatcttttc ctcgaattat gaccgcctca ggatttaact ctgcgagatg
     4081 atccaatagc cgcagtggat cgcggtaagc tatcgaggca atgctcttct gacgcaattg
     4141 cctaaggaca aggtcggcaa tcgatgtttg ttcgataagg acaagtacta gcatggtatc
     4201 ttagttttgg cacccatgga taaaacccgc aaaagggact atacttcaat ccaccttcaa
     4261 tgacacagca tacgatgaaa aaagacaatc tgcaagcgtt attcgaaaag atatccagaa
     4321 atccagcatt gggagaaatt cagaagctga tagagcagaa ctcctttcct ctccatattc
     4381 agaattcaga gaattctttg ctcgctctgt cttttgctac tctggtaaag cgaaccaaaa
     4441 gaagattcct tgtcgttctt ccatcagacc atgaaacaga agagttttct caggatctag
     4501 cgcttacggg cgttgacctt ttgagttttc cttcatggtt tggagcgcct tatagggcta
     4561 ttcccctgta ttctcgtatg tttacggaaa gggcttctgc tcttgctagg ctcgcggaag
     4621 gagattttga aattgcaact gtttcggcgc gcactcttgc aattccggtt ccgccaccag
     4681 aatatatcaa aaatcatatc ttgccgctga aaatcggggc agatttcgaa ccaaccctta
     4741 tatccagcaa actagcggaa cttggatatc tgagggtacc ttctgtgagc ctgccgggcg
     4801 aatttgccgt gcgaggtgaa gtgctcgata tctatatgcc tggttatgaa tatgcagttc
     4861 gtatccattt tgactatgat cgtatcgaaa gaataatccg tttcgatcct gagacacaaa
     4921 ccgggcgcga gaagcttact caaatacacg tgagacctct caaagaactc gtgtgggaca
     4981 aaaccaggat ctatcagctg cagaacaatg ctacggattt catgcgaaat gatcctcgtt
     5041 ttaaggagat tatcgagatt cttgacagtg aaaagcagat gcagggcgaa gaactatggt
     5101 ttccgcttgc atttgataaa atgtacaacc tagtggatta tctcgataat agggcgatgc
     5161 ttgtgctagt aggcagcgag cgcatttatg caatttccga gacattaagg aaagaatatg
     5221 cctcaatgta tcggattgca cttcgggaag tcttagtgcc accgccaaat aagatccata
     5281 taagtccaga tgggctattt gctgctttct cctctgtgct gttctgtcat gcattgaagg
     5341 gagtcgatgg ctcagcagag cgcatatctt tggaagcaga accaccacac tcctatttcg
     5401 ggaatataag gctcttcaaa gaagagttgt cgggttttaa aaaagaaaac tataaaacat
     5461 ggataattgc ctccactgag ctacaagcag ggcggattag ttcgctcatc caggatgatt
     5521 ctattgttat cgctaaaggg cccatttctg ctggctttac cattccttct ctcaaattgc
     5581 gagttctcgc cgagcacgag ctctttggca ggcggaaaca tgtccctcgt tctattgcca
     5641 agacgaagag tgccgtaatc gagagcttta tagatctgac tccaggtgat tatgtggttc
     5701 ataacaatta tggcattggt aagtttgcgg ggatagagcg tctgatagtt ctcggtctgg
     5761 agcgagatta tatctgtatc gaatatgcag gcaatgaaaa gatctttgtg cctatcgagc
     5821 aggctaacct agtacaaaga tatataggca atgaaggcca ggctccccat ctcgactcgg
     5881 ttggatcaaa agtctgggaa aacagaaaga aaagagtcag taaatcggtg gaggagcttg
     5941 ctgagcgact tatccggatt tatgctcgtc ggaaaaacgc ccgtggtttt tcatttccat
     6001 ctgataacga atggcagatc caatttgagg cagcatttcc atatgaagaa accgaagatc
     6061 agctaaaatg catcgaagag gtcaaacgcg atatggagag ctcgaagccc atggatcggt
     6121 tagtgtgcgg tgatgtgggc tttgggaaga cagagattgc catgagagca tgtttcaagg
     6181 cgatcctcgc aggcaagcaa gttgcatttc ttgctcctac cactattcta gccgaacagc
     6241 atttcgagaa ctttctcgag cgggctggag attttccaat caaagtggcg ctgctctcac
     6301 gactggtcga tagaaaaact aagaatgctg ttataaaggg attagcggat ggaagcatcg
     6361 acatggtaat tggcactcat aggattcttc aaaaggatgt acgtttcaaa gacttagggc
     6421 tactcgttgt cgatgaagag cagcgttttg gggtgaaaga caaggagcgc ttaaaggaaa
     6481 tgaaggtcgg tatagactgc ctaaccttaa cggcgactcc cattcctcgt acccttcata
     6541 tgtcgctact caagattaga gatatgtctg ttctgcagac ggctccgttg gaaagacaac
     6601 caattcagac ttttgtagag gaattctctc ctgaactcgt cgcccgcgca ataagaaatg
     6661 agatagcgag gggcggtcag atattttatc tccataatcg cattgaaaca ctgcccgaag
     6721 ttgagtattt tatccggcag cttgttccag aagccatagt agagagtgcc catggcaagc
     6781 tcaatggcca agatctagag tcgataatgc atcgctttat ccatggagcc ttccatgtcc
     6841 ttgtttccac tactatcata gaaaatggta tcgatattcc aaacgtcaac actataatta
     6901 ttgatagatc agataattat ggtatttctc agctttatca acttaagggc agggtaggac
     6961 gatccgatag acaagcctat gcctatcttc tatacccaga taagagggct ctcagcgaaa
     7021 ttgcgatgaa acgacttcaa ataatatccg attttactga gcttggctct ggctttaaga
     7081 ttgcaatgaa agacctcgag gttcgcggcg cggggaatct tctaggacgc gagcaatccg
     7141 gtaatatata tgctgttggt tttgatctgt atctaaaatt gctcgacgat gccgtatcta
     7201 gactttcagg catccagaat gaggaagaag agccctatct ggagctcgag tactctggtt
     7261 atataccaga tagctatatc tctgctccga tgattaaaat ggaaatctat aagcgcatcg
     7321 cttctgtttg gacacagggg gagattgatt ctctacatga ggaacttgaa gagcgttttg
     7381 gtccccttcc ggatgaggca ctctctctgc tttcgcttgc agaaatgaga gtcttatgta
     7441 gaaagctctc catagggcgc ctgcaggaac gagcaggaat cgttaccgtg gaattttcga
     7501 aggtctcgaa gatttccgcg gagaaaattc tgagacttat acgtgagtct gcaggcacaa
     7561 tccgcctatc gacagaaaag ccaaatgcca ttcggataca gacaaaagca attggcttaa
     7621 aggagaaaag cgcttatctc aaggagcgtc tttctctcct agctgattga agctgcttct
     7681 tattgagctt tgcgggtatt ccaaagcaaa acacggctgg cttctctggg attataatgg
     7741 ctttatttcg ccaagatgca atgggcatcg accttattag tggctgatta gttccaagat
     7801 tgcatgcaac gcaaagatag agattttcag gtagtgcttc aatacaagct cttaatgtcc
     7861 tttcattacg gtatggagtc tcgatgaaaa ttcttgcagc tttatcgtgc atggcagctt
     7921 ggccttcaag gaccagttct ttttgaagct cttcgcttcg tattggcatg tagccgagaa
     7981 aacagaagcg ttgaccattg agaccagaag cagcgagcgc gaggagtaag gagctatcgc
     8041 ctccgattgg aacaattcgc agctgctctt catgtgctag agatacaagc gccgcaccag
     8101 gatccgctat gcaaggaagc cccgcctcgg aaagtatggc acagtcataa ccgcttttca
     8161 gtgcagaaag cggagccttg agatcctctt ctttcgagtg ctcattgagg ataaaaaacc
     8221 cgacctcaga gaattgctct ggcgaaagca atcgggagag cagtcggttg gctgttttct
     8281 ctgattctac cacaaaatat ggaacgcttt tcattatggc aataacattg ggggctatca
     8341 tattcatgag ttgacaaggc ttccaatctt caggtgaata aggcaataaa ggaaccggta
     8401 aaagataaag ggtaccgcgg tttttgtcat tctctgacat gatacctctc atttagtcct
     8461 tagctataat acacttgatc gctgcaggtc ttcgagtgcc tgcgatatca gcggaaggaa
     8521 aagctccata ttccagaatt tggtacgatg gaggctctcg ctatacatcc ttaagacgga
     8581 atgaaaaaca cgttccgtca ggggataggg cgaagaggta attctttgct cagcatcata
     8641 atctcggaat gcacgctgtc cggctataac cagcctaaaa taagaaatcg agttgcgttc
     8701 gatggtagag gccgcaacta gatatgcacc atctccgttg gggaaaatct ccgcacctgt
     8761 ccttctaata aaaaggcgat cgatagattg aagcggaatt ctaattcttg tgagaaccct
     8821 tctggattcc agagaaggca agccttcttc attggtcagg taccatatat tcttgatgct
     8881 tgttcctgag ggtcccttga actcaagttc tgcatctaag catgccaaca gaggccaaag
     8941 agcaaggaat ctcttttgat acataagatg tccacctata gttgcaatgt ttcgcactgt
     9001 ggagtttgcg gtggctctca atacttctct ggcaggtttt ttttcgaatg gatatacaga
     9061 ttcaaggtcg tttagagcac atgcagcccc gaaggatatc atccgctcag tcttatgaat
     9121 ggtttttagt tcgggtatat ttctgatgct tgctatagaa gggtcaaaaa aaagataacg
     9181 tgtgtcttga atatatccga agcttgttgc gccagcaact atctgcagat ttttttgtcg
     9241 ggccattatg gctatgagtt cttggatact acgcggataa tagatttcgc taaggttcat
     9301 ggcgtttttc tcgctatggg caattgctgt attactttgg agagcgcatc agcaaaggct
     9361 ctgggatcaa tatggctgca atgcaagctg gcaaggatat ccgatatctc atgccatgag
     9421 ggagataaag atctttgaat caaatgaatt gctactagca tcagcgaatt attgcagtaa
     9481 gcacaggaca ataggccctc cttatcgaac tctgcggata gaatgccata ttccttgctc
     9541 ttggtaaaat tttcgattgt ctctatatgc ctttcccgca aaagaaaggc aggcaggata
     9601 tagctctgta caagcctacc atcatataga ataaggcaat tactgcattc gctatcagga
     9661 cagatctcgg agaggctctt tattccgcat tgatcaacaa gcacgtcgaa ggctctttgt
     9721 acgctagtaa ttgcgattct ctgcagattg ccattcaaga aaaactcaat gttcatcatc
     9781 tttctccaaa agatcgctag agtacattgc ggtaggggta ggcttgagcg gcaatataag
     9841 cctttccgag ctgttcaatt gttgcatcac ctcgagaaag catgagagta cgagggtata
     9901 tgcgagatct ccgatagagc gctcaaaatt ggccttttcg tctttcataa gttctatatt
     9961 gattctgccc tcagaaaaga tccatttata tatatcatct atgtttctat cttgataaat
    10021 gcttcgggaa aggcatgcaa ggagagcttc gatactcgca gagcgaattg caccctttgc
    10081 ggcggattgg cagagaattt ttccgacata aatcgatata tcaagacgaa ggctatggaa
    10141 gatggaccta atgctatcat aatcgacctc aaggattgcc gctccttgag atagatgtct
    10201 tatacttttg gattgggaat cggagacgtc actttgcttc atcgatgaaa atatctggat
    10261 tttaatggga aggccttctc catatcgttt atagctaatc caattatttc ttatttttgc
    10321 cagatctgcc attgttgaaa tgcaagacga gtcgatatgg ctttgttcag caatagacaa
    10381 agagatggct cgtattctag tctcttttgc tccatggctt ctcttatgaa tttgctcata
    10441 ggctgcgtat ttccgatgaa aatccgtatg ttccaataaa ggtcttaaga ttttttcggt
    10501 agaagactct tcctttggtg gcatcttatt cataattgag gaagaatcta ttccgctaaa
    10561 ggtcatccat agtaattcta ggagctcgac atccattttt cttgcggcgc gagcaatatg
    10621 agtctccagc atgctaaata tcgcagatga tgaaatacag tcgagagctc ccataggaat
    10681 catgttggtt tttaccgtaa agccgattat agtcaaagga atgttgggca tagcatccgc
    10741 aacgaggcgc acagtctttt ctaatatcag ctcatctaag acagaataag ctccaacagg
    10801 aatcgcaaag cgacatgcaa catccataag acaatgtttc tgcgttgacc accttgtttt
    10861 catatgaaga gatataccat gtataccagg taggaatcgt ttttctcgtt gaatagatat
    10921 aaggagcttt ataggccttc ctagaactct agcagctgct gcgcttctgg ctgcaagcaa
    10981 tgaaggatac cagaaatata tcccggaatt gtcatacatt ttagattgaa ggatttcgat
    11041 atcctcagtc gagaggccgg ttaactctgc aagacatgag tgtataagct caggcgccag
    11101 agcggggcaa ctgagtttca atctaccgtt ttcccattct gcgagagcac ccaggccact
    11161 atgaaagcta aattcatggg gctccagacg caaataggaa gccaattcaa gactatcttc
    11221 ggcttctgag gcttttaggg attcggaaga caatttctct tttccccgcg aataatcaat
    11281 tcgggctgaa atatctgaag atgaaaatac ttgccagtcc agtcttgatt cttgtgcttc
    11341 gcagtcgact atcgtattca atgccagctc ttccgtaagc aatggatccg gaccaacgat
    11401 tagcccaacg acttctccta tgtaggagac ctttttggct gctaacaatg gcagactcat
    11461 gccttctatt ctaagttcgt ttttaaatgg caaccgatca gcagtgataa gagtatagcc
    11521 ttccggcaaa ggaggaacat tgatgttctg gatatttcca atatttgaga acgcccttat
    11581 ggcaaaggca tatagcatat cttgcgccaa gagctgttca aatgggacag cttcatctct
    11641 tgtcatgata tacacctagc ggagctgctt tggctgtttc cgcttccatg acttcaaagg
    11701 ccaggtcgag taatataata gagtctgcta tatcgagcct cacttttgcg cgacgttttc
    11761 ttctattcac cttaatgata aagccctcaa taccttgtaa aggtccttct attaccttta
    11821 ttctctgatt ttcgtcgaat cttacaagcg atggcggaat caagcttcca aaactgatga
    11881 aatggcgaat aatttcgcta tcgcgattgt tgagtggttg cggaccttcg ttgttcggta
    11941 ggaatcgtat gaaagacttg gaatgcctca gccgatatac tatctccaaa ggcagagaat
    12001 caccatcata ctcgaaaaat acgtagcctg gaaaaaccgg tgaattgtca agtctggctt
    12061 tccccttttt tcttatgtac atcttcttca ttattttatg gaattttata tctggtactt
    12121 gatgttgaat tcgctcaagc cattcctctt ctttgcatgt tattacctgg atagcataaa
    12181 aacgcatgtc gatagaatag catatcttga atatccagtc agtaagaaat gtggaatagg
    12241 atcttatctc aaagtaattt gacaggcttc ctttggatga ttagtataag atcatgccag
    12301 atgatttgag aataagtgaa gatcctgata ttcggcagaa gagaatcaga gaattggagt
    12361 atctgatcag gcaccaccaa gaactgtatt ataacggcga acccgagatt tcggatgaag
    12421 aattcgatgc attatgggat gaactcgctt cgatagatcc agcaaatcct attattgctt
    12481 ctataggagc agacagatca gatagatggc ctaagtatag gcataggatg ataatgggga
    12541 gtctctcaaa ggcaagcgat ccagagtcat ttttagcctg ggcagctaaa gtcagatatc
    12601 cgctttacct cgtgcaatat aagctcgatg gcgctagcat agagcttcaa tacgatgaag
    12661 gtggctttgc aagagccgtc actcgtggag atggggtaat aggcgacgat atcactccca
    12721 acgcggtgcg tatgaatgga gttccgaatc ggcttccaga gccatttaca ggatcagttc
    12781 gcggtgaagt gctcatgtcg aagagagtgc atgcatccaa atattctgac aaggccaatt
    12841 gtcgcaatgc cgcaaatggt ctcatgaaac gcaaagatgg tcagggagtc gaggatcttg
    12901 atatcgtatg ctatgatgcc gtgggcagat tgcccactgg cgaagccttc tccaccgaac
    12961 gagaaaagat tgcctggctg aaaagaatgg gtttcaatgt tgttccaacc atcgaatgta
    13021 agagtcctga ggagatcatt gtatatcgtg ccaagatcat ggagttacgg cctactctcg
    13081 agtatgacat agacggactt gtagtaaagg gtgatcgaat cgatttcgaa gatatgaaca
    13141 agatgcgtcc cgagctacag atagccttca aattcagtcc agaagaggct gttacgacac
    13201 tcatagcagt tgaatggagt gagtctggga ccaccgttac tcctattggt atagtcgaac
    13261 ctgtgaggct tgcaggaacc actgttcagc gtgccaatct ggctaatcca ggcataattc
    13321 gctctatgga cttaagaatc ggttctaagg ttgtaataac caagcgcgga gagatcattc
    13381 ctaagatcga atctctggtc gaaaacccca aagatgctat tccgattcct attccttcac
    13441 attgctcgtt atgtggcgca gggctaatcg atgaggatac gcgtctttat tgtccgaatc
    13501 cagaatgtcc agggaaagcc tatcatcgcc tcgaaaaatg gctttctgta attgatataa
    13561 aagatatagg atcgacgttg ttgaagagac ttttcgaagc taagagagtt gtgcgcatac
    13621 cggatctata ttcacttaca atagatgacc ttatctcctt tgagagaata ggccggaaga
    13681 gcgctcagaa gatattgcgc aatataagag aacgaaatga agtcagtctt tctcagttta
    13741 tcgcaggttt tgatatagaa ggaattggct tgctcatggc agagaagctt atagaagctg
    13801 gatatgatac tctagataaa attctagctg cttcaccggc ggatttcgag aagatcgcgg
    13861 gttttgctga aatcagcgcc aagactcttt acgaagggct gcgattggtc gaggaagata
    13921 tgcgcaagct cgtcgatagt ggatatgtgc gaatcagacc tccgctccct aaaacccaga
    13981 ggaccgcaaa tccagtctcc ggcaatagtt tttgttttac tggcgagctg agatcgatga
    14041 agcgctcgga agctgaaaaa ctggtccggg aaaagggcgg tataataaaa tccagcgtaa
    14101 ccaaggatct tgattatctg gtcacgaatg atccaagctc aggatcggaa aaaaaccgca
    14161 aggctaaaga attatcgatt aagatcatcg atgaagagca attccttgag atggccggcc
    14221 ttaaatgatg gattttgaat gagaacacct tcagcaaggc ttctagagca tctcgatatt
    14281 ttaaagccag ccctccatga agagaaggag gggatagtac tcgatgtcta tgttattcct
    14341 cgtgctcctt acgatagtgt agagggactg cgcgatgggc gcatattgct gagagtaaag
    14401 gcagcgccgg aaaagggaaa agcaaatgag gccgttttag acctgattgc ggattatttt
    14461 gatatagcat cctcgaatct tcgtatattg cgaggtcata gttcaaaacg caagtccatc
    14521 ttattgcaaa gcttcagaga aaatcgctaa aatggagctc ctcatggaat attttgtgga
    14581 tcaatggaaa tcgataattg caaactgcct gaatgagctc gctctccaaa aagagctcaa
    14641 ggatgtctct ttttcatctc aggatatcat tgccgagaag ccacctaggc cgaagcttgg
    14701 cgacataggc tttcccatgt tctcatatgc aaagcaattt cgaatgtcac cggatagaat
    14761 tgcagcggaa atagcgagtc tctgcaacat gacaatcgat attgagaaac tcattctgcc
    14821 tgggaccatt aaagcaacag gttcatattt aaacatatat ctagacaaag aatatgtgac
    14881 gaattatgtc cttgaaaatg ctgagaaaga aggttgggga tctagcccac tttttaatgg
    14941 tcaaaggata atgattgagt tttcctgccc taacaccaac aaacctcttc accttggcca
    15001 ccttcgaaac aatgtgcttg gagagtctct ttcgagaatc atgaaagcag caggggcaga
    15061 agttaaaaag gtcaatctga taaacgacag gggaattcat atctgcaagt cgatgctcgc
    15121 atatcttgcc tatggacaag gacgtacacc ggaagacgaa ggattgaaaa gcgatcactt
    15181 tgtaggcaaa tactatgtct tattcaatag attaaaagaa gaagatcctt ttgcagaaga
    15241 aaaagctcaa gaacttctgc agaaatggga agctggggac ccagatgtaa ttgcactatg
    15301 ggaaaagatg aacaaatggg ctgtcgaagg cattatggat acctatagac gccagagggt
    15361 tagtttcgat gaattccatt tcgaacacga gacctataaa ctgggcaaac aggaagtgct
    15421 ggaaggcctt gctagaggaa tattctatcg tgcagaggat ggtgcaattc aggtagatct
    15481 ggaagatgtc ggtctaggaa aaaaagtact cctccgaaaa gatggcacga gtatctacat
    15541 cacgcaagat atcggaacag cgattatgcg ttacaacaga tggcctttcc atgagctcgt
    15601 gtatgttgtg gcctctgagc agcaatatca tttcaaagta ctttttgaag ttctaaggag
    15661 gcttggttat agctgggcag agaatctcta ccatctctca tatgggcttg tgaatttgcc
    15721 ttccggcaga atgaaaacca gggaaggaac ggttgtagat gccgatgacc tgatagacga
    15781 acttacagaa cttgcagcaa atgaaatagc ggataaggga agggaggaag cggtgggcga
    15841 tatcgaagat gttgccgagc aggttgccct tggagcctta cattattttc ttctgcaagt
    15901 ttcgccgggc aaagacatgc tctacaatcc tgagcaatct ctatctttta ctggagatac
    15961 ggggccctac attcagtata tgggagctcg agccagctct attcttagaa aacacaaaaa
    16021 tggtgagggt aatgcgggcc aagggaaaat cgatgtttct cttatagtgt cggattccga
    16081 ttggtctctc atgaggctat tgatggactt tccctatact atcgagcagt cggcaaaaaa
    16141 caaggatcct tccatacttg ctgcttatgc gcatcagctt gcgagcgaat tttccgcctg
    16201 gtatcgagac aatccagtgc tgaagaacga caatcctgat atctcggcca gccgcttggc
    16261 gttagtgaca gcgattaaag aaatactccg aagagcaagc aatttgttat gcataccatt
    16321 tctcgacgcc atgtaagaag acccggatac tgattcgctt tcttttggtt ctgagagctg
    16381 ttgacaagat gcggtcataa agtgtataaa ttgcagcctg atatgaatat ttgaggattt
    16441 atgaggataa gctaaatgta cgcaatcgta gaaatcaacg gaaaacaata tcatgcagag
    16501 caggggaagg atctggtagt agatcgcttt gatgcaatgc caggtcagtc gattgtcttc
    16561 gaaaaagtgt tattgcttag tggggatgaa acaaaagtcg gaactccata tgtcaatgat
    16621 gccgctatca aagctacagt aagagaagag atcaagggtg acaagataat tgtatttaag
    16681 tataagccaa aaaaaggata tcgcaaaacc cgtgggcatc gtcagcctta tacagtgcta
    16741 acggtcaatg aaattgtggg cgcttgaggc gtccatctta accggaggaa tataccatgc
    16801 cacataaagg tgttaatggc cgagattcaa atccgcagtg tcttggtgtc aaggcctatg
    16861 ctagcgagag gataagcgca ggttcgatta ttgtacgaca gaggggttcg aaaataaatc
    16921 caggcaagaa tgtaggtaag ggtaaggatg ataccctatt tgcgcttatc gacggtacag
    16981 tgcgcttcca tgaatataaa ggcaagaaat ttgtagatgt ggagccagcc attcaagcat
    17041 gaaggtaaaa ctagagcata attctaaccg ctcctgtagc acagagggcg gttttttatt
    17101 atgctcacaa taatggagaa tttctgtgat aaaattcgct gatgaagcga ttataagcgt
    17161 cgcaagcggt aaggggggcg atggttgcgt tgcctttagg cgagagagat ttattccctt
    17221 gggtggccca gcaggcggag atggggggcg cggtggagat gtggtgtttg ttgtaaagag
    17281 gaatcttcgg acgctcgcat atcttcgata tagacaggtg ttcaaagccg aaaacggtca
    17341 gcctggcatg ggaaagaaca tgcatggaag agatggaaag gatgttataa tagaagttcc
    17401 gcctgggact attcttcgcg acgctcagac aaatgagatt attagggatt ttgtatatcg
    17461 ggcaagaggc aaaaagcttg gggctctgga ggatgaagag ccatggattt ttttaaaggg
    17521 cggcaaagga ggctggggaa atactcattt taagagctca gtaaatcaag cgccgcgttt
    17581 tgctcaaccc ggacagccag gacaagagag aaagctcaaa gtcgaactga gcctcattgc
    17641 ggatataggg cttgttgggt ttccaaatgc tggaaaatca agcttactag agtattttac
    17701 aaacgcacgt ccaaagatcg ctccatatcc tttcacgaca aagataccaa atttgggcgt
    17761 tcttacggtt aatgagaggg atattatctt ggctgatatc ccaggcataa tagagggagc
    17821 acatgaggga gctggattgg ggcttcgctt tctaaagcat atcgcaagga cagcctgcct
    17881 tgcttttctg gtggatcttt ccgatgagca ctggaaagcg gcttttccta ctttattgcg
    17941 cgaattggaa ctattttcgc caaccctggt gaagaaaccg cgtatcttgc taggaacaaa
    18001 gatggatcta tcggaatccc aagcaaattt tattgatttc aaagcgtctt tcccagaaga
    18061 gagcatttat ggaatatctg tttttaccgg tcaaggtctg gatgaggtca aacagcaact
    18121 ctttgaaaag gttttggcct atgaggctgc tgagaattat gaatctcaaa aagaaaatgc
    18181 caaggagcgc atattcgatg aaatagattt ctcggacgag ggctttattt aatgagaatt
    18241 gcaattatag gagggtcttt taatccgcct catatcgggc atatgattct gattgaagag
    18301 attcttgcca ccggtcgtta ccagagagta ttgcttattc cagccaagac tgcaccccat
    18361 aaagttcctc agatggaccc aggtccagaa atgcggcttg ctctcttgga agcaagcatt
    18421 gaaggatggc ctaaggtggc aatcgaagat tgcgaattga agcgaactgg tctatcctat
    18481 acttcagaca ccctggaaga actatgggac agaaggctgt ttgatgaaag gcccgggctt
    18541 gtgataggcg atgatctagc tcctgatttt ctagcgacat ggaaacgagc cgaaaagatt
    18601 ctggaattag cggatattat cgttgcccat cgtctctatg tcgagaatat cccattgccc
    18661 tatcctcatg attatctcga taatcttgtt attcccattt cttccacgct tgtaagaggg
    18721 aggattcatg gtggcggtgc atggcgttcg ctcgttatgc cttccgtccg cacaatcata
    18781 gagaattatg gactatatcg aaatacctga aattgcgtca ataattgaaa aagagattgc
    18841 ctgcaatttg tctcccaaga gatatgctca ctctcgatcg acttcagaga ccgcggtaaa
    18901 attggccgaa cgttttggtg aagatcgatg ggctgctcat ctggcgggat tgtcgcatga
    18961 tatgtgcaga ggattgagtt ttgaggaaca aaacaatctg gtaaacaaac atagagcttg
    19021 catcgctttt ctaagtgagc gcccatctct agcagcaatc ttatcggata gagaatatag
    19081 ggaaaagatg attcatggcc cggcggcagc ttgttcgctc tgttataggt tccaggtcag
    19141 ggaagaatcg atattggaag ctgtggcatt gcatagcatt gcagatgatg ctatgtctcc
    19201 tcttgctaag attgtataca tagcggataa gcttgagccc cttcgcaata gatcagcgga
    19261 tgcagatgaa aaaatgcaga ctttggacct ggattccctt tttgcatata cgcttgcttc
    19321 agtcgtaaga tggttttctg aatctgggaa gccgctttgc ccatacactg ccgagatata
    19381 tagtaggatg acaaaatcat gagcgactct aaatcgaaaa ttctaaagcg gagcaccttg
    19441 ccacttttag tgattttgat cgttgcggct gtcgggattt tctttgtcgt aaaactcacc
    19501 gagaagggaa ctgtcgagag cattatcaag aaagaccagc ccatggctat gctctttatt
    19561 tttgagagta ataagaagcc tgtttctaat caacttttaa tctggtatcc ttcgaggcgc
    19621 aaggctgcga ttatggatat tcctagcact atgggcatca tcttgaaaag cgcaaataag
    19681 atgagcagca tcgacactgt ctatgactct cgaaatccca gcaaatttgt caaagaaata
    19741 agtgattatc ttaaataccc gatagatggc tggtttgtat acgatgaggc tagcctttgt
    19801 caaacaatag atctgctaga aggaattcag atgtttatcc ccgagactgt gatggagagc
    19861 gactcattga aaggggtgtc tctgccagga ggcgctgtag tgctagatgg ggataagaca
    19921 aaccaatatc ttctttatgc cttggctacg gattcatatt ccgaggagat cagccgtaaa
    19981 caaaggttgc ttattaacat gttggggagg cttgccgatg aatcagcaag tctcaacaaa
    20041 aagagtaatc ttcgtttgat tgcctcaaag ccaaagagca atttcagcct tgcaacccgt
    20101 ttgagcattt ttgagcactt agctcagatt gatcatgata tgataattac gcagttcatt
    20161 tccggatctt ttagaagctt cgaggggcgt cgccttctct ttccatatta tgatggagag
    20221 cttgctcggg atgtggtgag tcaaacagca aaggcgctgg gtatagagga agatggagct
    20281 tctcagaaga cgattgtgac aatagacata ctgaatggct gtggagaaaa agggcttgca
    20341 aatacagcat cgattctctt cgagagctat ggctacaaag ttgtatctgt aggcaatgca
    20401 ccaagcttcg attatgcaaa aacagtcatg tacgacaacg atggagataa ggcagcgctt
    20461 cagcaggttg caaatattat aaattgcaag aattttggcg atgcttcagt tctgccaggg
    20521 cttcaaaagg ccaatatcac tataatattg ggaaaggatt tcaacgggag gtattgtatt
    20581 ggacagtaag gcgtttgcat atcagagtgc tgagattctg gcgcaacata aagcgctgaa
    20641 tgttctcgtt atggatgttc atgagatagc tgggtgggct gactattttg tactggccac
    20701 atgcacaagt tccactcata tgcgagggct tcaaaagcac attgaggatt ttctaaaagt
    20761 ggaaaaaata gatcttctca ataagcctga agttacagac gatcagtgct ggctcttaat
    20821 ggatgcccag gatgtgatta ttcatatcat gagcgctgag gccagggatt tttatgatct
    20881 ggaatcactc tggttcaatg caccccgttt tgcagtcgct tttcgtggaa atggaatctg
    20941 aatccggaat ggaatagtgt cattaatctt ggtatgagca agatatttaa aaggcgcggt
    21001 tttcaccgcg ccttttgatc tataagacca cgctcttatt catccgtgtc gtaatcaaaa
    21061 tcttcgtctt cgaagtcctc gtcctcgaat tcctcatcct cacctatgtc ctcatcttcg
    21121 tcgaaatctt cgtcgatatc ttcctcgtcc tcgaattcct catcctcttc gaagtcatct
    21181 tcatcttcca aatcttcttc gtcatcaaac tcgtcatcat cgctgtcata gtcgaattca
    21241 tcatcgtagg caccatcgaa tcgcacagtt ggtcgaagcg aatatatggt atcggcagaa
    21301 tcgccattgg taagggtgat caattcgaat tcttcctgcg tatcggctgt cattttaatt
    21361 ttatggttca tattgcgcac ctcccataaa tattctcttt gtattgaagc agatgtacta
    21421 ttacgagaaa cagtcagtgc gtcaatacag cggcaaggca aaatttatag gtcaaatgaa
    21481 aatatgtcaa ggaaaaatcg caaaaataat gctttagaaa tggcttttaa cagaaaaaat
    21541 tgtcatatat aataccatga aagatgccta cactcgaaat taaggtcttt gcaaagatca
    21601 atatcggact taaggtattt cagagcagaa cctctggata ccatgacata gaaagcatat
    21661 ttcagaatgt cagtctagcc gatactattt ggatatcaac cagcaataag agcaataaag
    21721 caagagtaac catagaaggt gactttggat gccctataga atcatcaagt gtatataaag
    21781 cagcgattga attcgataga gctaccgatg gaatcataag acgaaacggt gtacatgtcc
    21841 aggttggaaa agaaattcct gcacaggcag gcttgggtgc agccagcgct gatgccgcag
    21901 ccacgctttg cggcttaaac gaactctttg gcaatcgatt ctcaaaaaaa gaactcgctc
    21961 gcatttcaat aagaatagga agcgatgttc ccttcttttt gtttggggag ggtgcagcca
    22021 tcgttagggg gcgaggcgag cggataagtc tcataaaacc aaggactgat tttggaatag
    22081 tcattttgct gcctgcatgg gcttcttcta ctaaggatgc ctataggctt ttggatgtta
    22141 tgcgccgaaa tggtctttct cggattcatt cctcgaccga ggtttgtgag ggcgaatcaa
    22201 cttatcgtgt ttgccattcg gacggcgatt tgaagaaatg gtatgatggt cccattgcct
    22261 attggcatct gggaaacgat tttcagcagg taatggaaaa acagcacaga gaatatgaag
    22321 tgtggtcgca gttacttatc gataatggtg cggtctgtac aagccttact ggctctggct
    22381 cagctatgta tggagttttc gaatccatga gagaggccga tgcggctgcc gaagaatgca
    22441 gaaatctgat caagagagcc gagaaatcgg aattgtgcaa tattgttagc attttctcag
    22501 tagtaccgct tgcgcgaagc atgattgtaa gttatattca agcttgcaac gtagataca
//
LOCUS       DAOMZL010000024        22365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_1995_length_22365_cov_17.390184, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000024 DAOMZL010000000
VERSION     DAOMZL010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 22365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22365
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1995_length_22365_cov_17.390184"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..701)
                     /locus_tag="PLQ30_03445"
     CDS             complement(90..701)
                     /locus_tag="PLQ30_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPW01531.1"
                     /translation="MYKAVIFDFGNVLCGVERENFVLKAAIHAKAQNAQDLLRTLWGT
                     PIEYEFETGIIDSHEYYRRVQRLVELEPGYSYEEFVRDYQSIIVPNPDGEQALKTAAS
                     MGARTFVLSNTSFLHASLIFDNEVLGTYPEIYIFSYKIGVMKPDPRIWMKLLEYIRLD
                     PGDCLYIDDIADYCKAAQDLGMDAFCYDFRIHNLSLELQNMLQ"
     gene            complement(779..1486)
                     /locus_tag="PLQ30_03450"
     CDS             complement(779..1486)
                     /locus_tag="PLQ30_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01532.1"
                     /translation="MSQGRRHPLLAGYYWILLFLGWITITILPLGAQDSYSAALSLFW
                     DGRRLEMAETRKAESQLAFQRSLAMTERLLSKEPSNPDLQTLKTWNLFRLHRYVDTVV
                     YAQSVLNTREDHRVLETMAEALFFLNKNEEALSAFGRYFTIAPENDDRRSSAFYYVGE
                     IYFRIKKYEHADIAFSTAVSMEKNMYYWWYRLGLVKEILGQYRRAYEAFNTSLSLKSN
                     FKPALEARERVKAKSSL"
     gene            complement(1506..2960)
                     /gene="gltA"
                     /locus_tag="PLQ30_03455"
     CDS             complement(1506..2960)
                     /gene="gltA"
                     /locus_tag="PLQ30_03455"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPW01533.1"
                     /translation="MEEAELELSRLRSGPITPKMRMQIPPQAMPAQDPIERRSNMNEV
                     ALGYSSAQAMLEAERCLQCKNAPCIAGCPVRVDIPGFIGKIKEGDFLGAARIIKRTNL
                     LPSICGRVCPQETQCQAPCTVGKALHGVEKAVQIGRLERFAADYERSRGAVEIPHVAQ
                     KTARKVAIVGSGPAGLTCAVDVRREGHSVAIFEAFHKPGGVMVYGIPEFRLPKVLVQA
                     EAEVLESMGVDFELNFLVGRTRPLLSLIKEDGFDAIFIGTGAGLPKFMDIPGENLVGV
                     FSANEYLTRTNLLKAYLKGRAGTPIYYSRRVAVLGGGNVAMDSARMALRLGAEEVRVL
                     YRRTRSEMPARAEEVEHAMEEGIIFDFLKSPTRVLGDDRGRVAALEVLSYELGEPDES
                     GRRKPVPIHGSEEIIPFDTVIVAIGNESNPLISKTTPELQVDRHGHIIVDDRQRTSID
                     RVYAGGDIVLGSATVILAMGEGRRAAQSINEIIQ"
     gene            complement(2981..3868)
                     /locus_tag="PLQ30_03460"
     CDS             complement(2981..3868)
                     /locus_tag="PLQ30_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPW01534.1"
                     /translation="MHNVVSKVQLSEEVFRIEVEAPLVARERKPGQFVIVMCDDEYSE
                     RIPLTIADADPDKGTVTLIFQTVGASTHKLALKNPADKIILLGPLGNPTHIECYGWTV
                     CVGGGIGSAPLYPIAKALRQAGNRVTVISGARTKDLLIMQDELRAIADEYIVVTDDGS
                     FGRKALVTEPLDELCRRAPPPDLVVAIGPPIMMKFCAETTRPYGIKTLASLNTIMLDG
                     TGMCGGCRVSIGGKTKFVCVDGPEFDAHLVDWDGMMTRLGTYRDIEKEAHDRCHLELQ
                     LEKLDAAEISASRPKESAQ"
     gene            4071..4886
                     /locus_tag="PLQ30_03465"
     CDS             4071..4886
                     /locus_tag="PLQ30_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HPW01535.1"
                     /translation="MPLPIVSQKLKDEALFKPLDFLAYLDKAGIALSQRPAPQTIVLS
                     YQKSLCDFVRATFPCERAKGYFGRFVHYVDVSDMDDSRDKHSPIGNKTIAIASDFGVG
                     APAAAVMLEELIAWGVKEFISIGFAGSLREDVPPGTLVVCDGAFRDEGTSYHYLDSNM
                     SVHPEPRLTEALENSLKRAGLAYAKGPTWTTDAIYRETKSEVQYFRDQGALVADMEAA
                     ALFAVAQYRNVSIASCFSVSDTLAYLEWRPEFHAEPSMQGLEMLFSCAIKALQ"
     gene            complement(4935..6578)
                     /locus_tag="PLQ30_03470"
     CDS             complement(4935..6578)
                     /locus_tag="PLQ30_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPW01536.1"
                     /translation="MKLLVLAAGIGSRFGGAKQIIEVGPNGETLLEYSIYDARKAGFD
                     EIIFLIRPDIEADFRSNILSRLPSDLRYSLAYQTLDFLLDKALAEINGEKQLDPRELG
                     QREGGLAESKRVKPWGTAHALLCAGELFKQGEAFAVINADDYYGRRAFEAVGSNLFNN
                     PGELCFAGYRLEDVVPASGTVSRAICTLSADGYLEKIIEHKKVWREGKRFFSEQGESI
                     VELSPDAAVSMNFWGLDYSIFEYAWQGWKEFLSRYIESPTQEFLLPDLIQSIVEKKRA
                     RVRMLPASERSFGLTNPEDLQEVRRRIFGFVAEGVYPSPLFGSWKIKSFPRLWHFADN
                     EYGECAEFEDLFMQAAMPWDVLKLLDSYLRSRLDAVSGEKIRSSIPKGVHIEGDVFID
                     EGCIIEEGAFIRGPAWIGKGCEIRSHCYIRGGLLAGSGVVLGHASEFKHCILLERAQA
                     PHFNYVGDSVLGHHAHIGAGVILSNFRLDGKPIRAKCLDSREKFETELTKFGALIGDN
                     CEIGCNSVLNPGTILGERSVVMPMSLVSGTWPLESRIDI"
     gene            complement(6669..7586)
                     /locus_tag="PLQ30_03475"
     CDS             complement(6669..7586)
                     /locus_tag="PLQ30_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein U domain-containing protein"
                     /protein_id="HPW01537.1"
                     /translation="MRRRFWLFLIICFAIVISMSAEKLTLDPISAPSPVTYDPFSGND
                     TRGQAFTFTVRMDKSTGTTSNFSVVINGESSPSSRSLKSSSGSIEIGFYKDSSHTTEI
                     RSTADYSPSYYISGSFDPKPDVLTQTFTIYPRLVKGQSAPYGTYTGTFTLLLYKKSSP
                     GQGNPEDTISFTYTAAVDKAVDVRVGSRNANYDTGATSYNIDLGEISNGASADFGIFI
                     RGTTGYTLTMHTASGGFLTSSNSEDKIQYTIIVDGKEYYLNSSIILDQETANCLYSKA
                     LLGTIKIPGDHGAKAGIYSDTVGFSMAVN"
     gene            complement(7613..10063)
                     /locus_tag="PLQ30_03480"
     CDS             complement(7613..10063)
                     /locus_tag="PLQ30_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HPW01538.1"
                     /translation="MLKKKNVYVLVWILFLWLPPIDAQIPQTPIVHDSIVPELEIQFS
                     ISINGKPIGDIETGIWHNEPILNKEIASRLLINYLRPDIYSVIFDTLFKELDWLTAND
                     FSLAGISFLFNELQLSLSIVIPPEYSPVVDIDFVPEQILNYKPILRPEPFAGYIQNES
                     DLHVSDISEGKVLFSTRLNSMIDVKGVHLFSDNFFSYAFGPSQVFSWNMNSVYLLWDN
                     TAIHSRTKIGKISIPAVSNQSQPSLYAMSLESFEDRQYMVRTGYIDNQTEFTIHKTAR
                     VSVEANGRLIRQVILEPGNYRILDLPFTTGLNEFVLRIEEIDGNVQVFRRIIPREASI
                     MQPGTSKYALSAGTSRTDFSEFIASGYYLYGFLSTLSGGINFQVDKRSAMGGLTFSTA
                     LPIGTLNGSGSIVGRWDGWGPVISPAATLSYLFSMPSKIYLPIFGISATFRGEGFLSP
                     NISSPSGTVPSAFLSFSTNFYTKIFANIGIGLSYSLYYTYSSPAKMTHEVFASISTAI
                     AAGGNLGLSGRLSFAESSAPTFSLNLSFTIMPKDDTTRVLNFMQSSGGQTNISINDKL
                     YMLGRVFDTNFGASDLLPGASKDGSMSLGIRNSSEYFDVSANGTFNYSPSSESYSLGG
                     YTQFRSSMAFAGTHVAFLRQIPDSFLLITASPELKDKDIVYRVSNGSQYQAAGGKNIV
                     VPIQSYETTILSIDLRATELNVAPRYSFITLSPAFRSGILFESDVIKRYFINGRLINQ
                     EGKPISYLPGDVFDFGGSLVTSTFTDELGDFEIYDILPGSYQIQWPEGFGTTQFELPD
                     TDEDRIDLGDIKLHIESK"
     gene            complement(10140..10877)
                     /locus_tag="PLQ30_03485"
     CDS             complement(10140..10877)
                     /locus_tag="PLQ30_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HPW01539.1"
                     /translation="MYKRQSCRKLILVAVFLISIAATATAFTFMPMSITISPSGANSI
                     ASFRITNDSSQQIAITIKAMMREIDENGNETNKPADNLFAIFPTRVVVQPNSFQNIKV
                     QYKGNPSLSREVAYRIIAEQVPIDFSQQQTSGVKVMFRYIAALYVAPPNVSHKVSVTK
                     VEYGEQESKKGFFVTITNSGTRHAIINDPVMKISGSSFTITLKDESVSAINGQNLLAG
                     NVRKFFIPCEDAVPGKNYTGTLTATIE"
     gene            11002..11075
                     /locus_tag="PLQ30_03490"
     tRNA            11002..11075
                     /locus_tag="PLQ30_03490"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11036..11038,aa:Val,seq:gac)
     gene            11093..11165
                     /locus_tag="PLQ30_03495"
     tRNA            11093..11165
                     /locus_tag="PLQ30_03495"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11126..11128,aa:Phe,seq:gaa)
     gene            complement(11302..12162)
                     /gene="nadE"
                     /locus_tag="PLQ30_03500"
     CDS             complement(11302..12162)
                     /gene="nadE"
                     /locus_tag="PLQ30_03500"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPW01540.1"
                     /translation="MPAEINPSQEKERIVEFIRTQIAKYEFEGAVIGISGGIDSAVVG
                     KLLTEALGRERVFALILPERDSSKRTVSDSLLVCRYLGIRYSVKSISPLVRRLGIYRT
                     KPPAFIFPRKIQEDYVRAQWQKTSSPYINDLKSAGGQSNRRNLAYYRTKNRLRMTILY
                     YEAEQRGYAVAGTINRTEFLIGFYVKWGDDSSDIEPIMHLYKTQVFELAREIGLPQRI
                     IEKEPSPDLAPGITDEFAIGLSYTDLDRILKKNELTLPLSDEDPELVEKVQKILAAVP
                     YRKIRNLSLL"
     gene            12292..13266
                     /gene="galE"
                     /locus_tag="PLQ30_03505"
     CDS             12292..13266
                     /gene="galE"
                     /locus_tag="PLQ30_03505"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPW01541.1"
                     /translation="MKILVIGGAGYIGSHVARMLADNGNKVSIADNLSTGTEENLFSD
                     YDFYKGDILDYPWLVSIMKHGFDAIVHLAAAKAAGESMIKPEKYATQNITGTINILNA
                     ACQAGISKIVFSSSAAIYGEPKYLPIDEAHSKEPTNFYGFTKLEIERLLEWYDKLRGI
                     KFAALRYFNAAGYDIEGRVRGLEKNPANLVPVIMEVAAGMRSKLEIFGTDYPTPDGTG
                     VRDYIHVTDLAQAHCKALNHLADARQSFAVNLGSEKGLSVLEILKAARRITGRPIPAS
                     IVERRPGDPAKLIASSCAAKQLLGWEAKYSDVDTIIATSWRVYEKHLA"
     gene            13313..14542
                     /locus_tag="PLQ30_03510"
     CDS             13313..14542
                     /locus_tag="PLQ30_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HPW01542.1"
                     /translation="MTELARAFEFLEAQERDIIQLESLLTAYPALAPESEGIGEIEKA
                     SALEAWLKAQGISNIEHFDAPDPRVPSKLRPNLVATIPGRRVEGPLWIMSHLDVVPEG
                     ELSSWESDPWKVVVKDGKLYGRGVEDNQQGIVSSVFAALAFVKQGIIPERTIKLLFIA
                     DEEVGSKFGIQYLLENHSLFGKDDIIVVPDGGSADGTEIEVAEKNICWLKIRTKGKQT
                     HAAMPDKGANALLAACDLALRVHRLEKSTFTARDPLFFPDRTTINPTKKEANVPNINT
                     IPGEDVFYFDMRILPVYPVSRMLEEVMKQAHAIEAEYNVKIELEVIQSNESKATPADA
                     PVVGMLAEAVKQVYGVEARPIGIGGGTVGAYLRNAGLDCVVWSKMNETAHQPNENADI
                     ANIIGDAKVFAALAMAP"
     gene            complement(14547..15962)
                     /locus_tag="PLQ30_03515"
     CDS             complement(14547..15962)
                     /locus_tag="PLQ30_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPW01543.1"
                     /translation="MRKIKSIGILCMLYAALSFASAKEIEGANSFQEADEYIVGVSSI
                     LEPPEELESTPIEETQESLDFSQFLPEEPSIEELSIEERAKSLQYDIPMPWGHELFEK
                     YRQYYLSNQGRKILLSTMEKAVPFMDYIQSKVTEYGVPQELAFLPVIESAFSPFAVSK
                     SGATGIWQFMKNSINGYGLTITEWVDERRDFMKSTDAAMKKLMDNYKELKDWPLAIAA
                     YNAGLGSLRRAVNTAGPGCDFWTIYNKGLVSRQALDYVPKFLAVASILRYPDLYGIEL
                     PSSGIKWETISLEQQVDIKLLATKAEIPLDTLKLGNAELHYTITPPENNHLLKIPADK
                     SDQVKTILADSNAPLVRYEIYKVKQGDTLTAISKRYGISISMITKVNPGINPDKIRIG
                     QTLMIPLSIQPLQSSGSSSNIANSGANIQTSIHTVQKGDTLYALARRYGTTAKDLANL
                     NGISLNSILRIGQRLKIPVRL"
     gene            complement(15959..17059)
                     /locus_tag="PLQ30_03520"
     CDS             complement(15959..17059)
                     /locus_tag="PLQ30_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW01544.1"
                     /translation="MKSARIGAFAAPVLISILIVFCLGLWGLQISGIGFSNLGEFPIS
                     NRSLRRELRCALEIFYSPQLKNSGYYSIQRMLAAKRIGTILIKDGQKASAARFLSSLA
                     DEHDDYEAWYLFAAGAVYESMDSDPIARILYERILKNLPDMMIDGQSIHRISLISLLE
                     GDSPPYKKAHWYGEFIRRFPEADEVGPYHFLLAKEYEKLGLWPQAIEEYRQFLPFFGV
                     SVPGYPDAHDYARKMVEFNDSPKDWTYADLNDLVSRIRKAIAKRSASELKKYMSKAGF
                     FAMSWYKEGGEESNSNVLFNFSNFMTRGPIYVSPAFDPSFNDSEALLRTSGWTGYIPV
                     WYLCFRKVNFPADPNVHGNWEWAGIYFGEKMQ"
     gene            17268..19262
                     /locus_tag="PLQ30_03525"
     CDS             17268..19262
                     /locus_tag="PLQ30_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW01545.1"
                     /translation="MKRNSIFLLAIAVFSVLSFLAPSWSYAQQTLPKTDVEQLKKIAS
                     EATDIETLIVLATPRSLSQARSRTKASPYLSEPNKAALDAIAKSIGLILYGPKNAPFE
                     IPVLAKGADQKYITCLAGLSDASAGKSPKIFENGSCSLMGELISALPFFRDSRNEIRA
                     GALAAISRLEDLGGISTVPPLVRGQIALEAKQYTEAASQFQKALELDALSEKPACGLA
                     RAWIALGEPGKAKQVLDPIISIALAESAKEISAKDGYNEDPSSNKVASSGGGYIETTE
                     AEQNILSADIKSLYGLALYHLNNILDAEPWLKAALKEDASRTDLLVPLAHAALQRRDY
                     ASAWKNLEGASKIASKDRNWLILKSQYALENSRQVDAERFARTAVSQYPQDPVALAQL
                     VRAIQESTDAQRHKEATDLAKKVLDITKNEPPNLTPLEQAWRKQAEDKAFQFLVMENY
                     NNQDWIMAAKYLQEAGGVPLDKEMVATILRKSGNVQEALQFALSWYIQEPTSEKAVEA
                     YLRSLAMATGATLASAAPTRDVPTAILPGMSGLEMRQEEGANLALLDIVLKFIAAPFS
                     KELKSFLYYMSATLQSDENKAIDQLKDALAERADNVEALVSLASIYLNRYNRQSDKSD
                     TINRDKAVLYLEQAKSLRPNDDEIKARINQLEAELGYSSR"
     gene            19310..20323
                     /locus_tag="PLQ30_03530"
     CDS             19310..20323
                     /locus_tag="PLQ30_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HPW01546.1"
                     /translation="MKNLSLQKSEKSSPQKDNERIASRQAHSPQKVSWQKPSYDPAIS
                     CFLEEIYAECNRSERLELDPLAIVRRYEKPEDMEVAGLLCSTLAFGSVELIMQACEKA
                     LKPLGEHPAKTLREMRHNEIEQAWAFFQYRFCFPKDMIALMSAIHEALDSYGSLEALF
                     LKCDTLSRQNTGKDPGDSAANEQKTHKGKTETDWQENILAASSTFVLKLHELALKAQP
                     EGLRKNLLPDPCKGSAAKRLFLFLRWMIRHDEIDPGCWNSISPARLIVPMDTHMIRTC
                     TERLRFLPRRKSAGTMASLRDALSVTEAFRLYAPEDPVKYDFALTRPGIDPRPGDERF
                     SCL"
     gene            20521..20594
                     /locus_tag="PLQ30_03535"
     tRNA            20521..20594
                     /locus_tag="PLQ30_03535"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20555..20557,aa:Pro,seq:ggg)
     gene            20710..22284
                     /gene="malQ"
                     /locus_tag="PLQ30_03540"
     CDS             20710..22284
                     /gene="malQ"
                     /locus_tag="PLQ30_03540"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPW01547.1"
                     /translation="MKIPRSGGVLLHPISLGSPWPCGTMGEEARFFVDFCVKSGLGWW
                     QVLPLGPTAFGDSPYQSSSAFAGNPYLIDPQELWAKGLLSSKEIESYRWKKGNKSRPS
                     FASPEAESSILSSLPEDLQYVDYGRLFTSNARLMDAAWLRYQAKARDEEAAAFDEFCK
                     ANMFWLDDYALFAAIKEKEQHRPWYEWPQEFKSRDPNALARFEKGNKELISRLKLTQF
                     LFFEQWIALKRYANRKGLKLIGDVPIFAAWDSADVWAHRELFQLDDMGRPIRVAGVPP
                     DYFTATGQLWGNPLYDWQAHERDAYAWWIERVRHLLKLVDALRIDHFRGFEAYWAVPF
                     EEPTAKHGRWERGPGHRLFDALKSALGELPIIAEDLGFITPEVVELRDSLGFPGMRIL
                     QFAFELEEDNPDYPHNYPQHCIAYTGTHDNDTARGWLTHAPVAAKKRALSYMHAKPST
                     FHQDMVRTVWASPAMLAIAPMQDFLGLGTEARMNFPGTTSGWWRWRMNREDLSPALAR
                     QIQRLSEIYFRTDASD"
BASE COUNT         6059 a         5226 c         5197 g         5883 t
ORIGIN      
        1 cacaaatcgt ttcatagaac ctcctcttgt acctgcggct gccattctag acacccgtcg
       61 cgccgcaatg tgcattttgt tcgctttatc tattgtaaca tattttgcag ttcaagtgag
      121 agattatgta ttcgaaaatc ataacaaaaa gcatccatcc cgagatcttg cgcggctttg
      181 cagtaatccg cgatatcgtc gatatataga caatcccctg gatcaaggcg aatgtattcc
      241 agcagcttca tccagatacg aggatctggc ttcatgactc ctattttata ggaaaaaata
      301 tatatttcgg ggtaagtacc cagcacttca ttatcgaaaa tcagcgaagc atgaagaaaa
      361 gaggtattgg ataaaacaaa agttctggca cccatgcttg ctgcggtctt aagagcttgt
      421 tcgccatctg gattaggcac gataatggat tgatagtctc taacaaattc ctcgtagcta
      481 tagccgggct cgagctctac aagcctctgc acgcgtctat agtattcgtg ggaatcgata
      541 atacccgtct cgaactcata ttcgatgggt gtaccccaga gggtacgcag aagatcctga
      601 gcattctgtg cttttgcatg gatagcggcc ttcagcacga agttttctcg ctcaacaccg
      661 catagcacat tgccgaagtc aaaaataaca gctttataca tggcaagtcc ttaaaattaa
      721 taattccgta gaaaacactt cataaggtcc gttcacattg taattttgaa aactgaactt
      781 aaagtgaaga ttttgctttt actcgctctc tagcctcgag cgcaggcttg aaattagatt
      841 ttagagaaag agaagtattg aaggcctcgt aagcgcgtcg atactggccg aggatttcct
      901 ttacaagtcc gaggcggtac caccagtaat acatattctt ctccattgaa acagctgtcg
      961 aaaatgctat atccgcatgc tcatatttct ttatccggaa ataaatttca ccaacataat
     1021 aaaatgcgct ggagcgtcgg tcgtcgtttt ctggagcaat tgtaaaatat ctaccgaagg
     1081 cagatagagc ttcctcattc ttattgagga aaaagagcgc ctcggccatc gtctcgagga
     1141 cacgatggtc ttctcttgta ttcaacacag attgggcata tacgaccgta tcaacatatc
     1201 gatgcaaacg aaacagattc caggttttga gggtctgcag atcaggattg gaaggctctt
     1261 tagataataa gcgttctgtc atagcaagag aacgctgaaa agcgagctga ctttccgctt
     1321 tgcgagtctc cgccatttcg agtcggcgac catcccaaaa aagtgatagg gctgcgctgt
     1381 aactatcctg tgctcctaaa ggcagaattg tgatggttat ccatccaaga aaaagaagaa
     1441 tccaataata tcctgctagt aaaggatgcc ttctgccctg tgacactcca cctgcctcct
     1501 ctcctttatt gaataatctc atttatagat tgagcggcac gcctaccctc gcccatagcg
     1561 agtatgacgg tcgcagaacc cagcactata tctccgccgg catagacgcg atcgatactg
     1621 gtcctttgtc gatcatcgac tatgatatga ccatgacgat ctacctggag ctcgggagta
     1681 gtcttagaaa tcagaggatt ggattcattg ccaatggcta ctatcaccgt gtcaaatgga
     1741 attatctctt cgctcccatg aatgggaacc ggctttcttc tgccggattc atctggttct
     1801 cccaattcat aagacagcac ttcgagggcg gcgactcttc ctctatcatc tccaaggaca
     1861 cgcgtcgggc tctttagaaa atcaaaaatt attccctctt ccatggcatg ttcaacttcc
     1921 tcagcccgtg cgggcatttc agaccgagtg cgtcgatata gaacacgtac ttcctctgct
     1981 cctaacctta gcgccatgcg agctgagtcc atggccacat tgcctccgcc caaaacagcg
     2041 acacgccttg aataatagat gggggtgcca gccctacctt tcaagtaagc cttaagaaga
     2101 ttagttcgcg taaggtattc atttgcgctg aaaactccta caaggttttc tcccgggata
     2161 tccataaatt tgggtaagcc cgcccctgtg cctatgaata tagcatcaaa accatcttct
     2221 ttaattaggg aaagaagcgg acgagttctt ccgaccagga aattgagctc aaaatcgacc
     2281 cccatcgatt ctaggacctc cgcttcagcc tgcacgagga ctttgggaag cctgaactcc
     2341 ggaattccat agaccataac gcctcctggt ttatggaagg cttcgaaaat cgcgacactg
     2401 tggccttctc ttcgaacatc gactgcgcag gtaaggcctg cagggcctga cccgacaatt
     2461 gctacctttc tggctgtctt ttgagcaaca tgaggtattt ccacggcgcc tctgctgcgt
     2521 tcataatctg cggcaaagcg ctcgagcctg cctatctgaa ccgctttttc aacaccatga
     2581 agagccttgc caaccgtgca tggcgcctga cattgcgtct cctgggggca aacgcgccca
     2641 catatcgaag gaagcaaatt ggttctcttt attattctgg cggcgccaag gaaatctcct
     2701 tccttgatct tgccgataaa ccctggaata tcgactctca ctgggcaacc cgcgatacat
     2761 ggagcattct tacactgcag acagcgctct gcttctaaca tagcttgagc agaactgtac
     2821 cccagagcta cttcattcat gttgctgcgg cgctcgatag gatcctgagc gggcattgcc
     2881 tgaggaggta tctgcattct catctttggg gtaattggtc cggagcgcag ccgggaaagt
     2941 tcgagttcag cttcttccat tgtagattct tctttatggc tcattgtgca gactccttgg
     3001 gacgagacgc cgatatctct gctgcatcga gcttctcgag ctgcagctct agatgacagc
     3061 gatcgtgagc ttctttttct atgtctcgat atgttccaag acgggtcatc attccatccc
     3121 aatcgacaag atgggcatcg aattcagggc catccacaca tacgaatttt gtctttccgc
     3181 caatggatac gcggcaacct ccgcacatac ctgtgccatc gagcattatc gtgttaagag
     3241 aggccagcgt tttgataccg taaggcctgg ttgtttcagc acagaatttc atcataatag
     3301 gcgggcctat agcgaccacg agatcaggcg gaggtgctct cctacagagc tcgtcgagcg
     3361 gttctgtgac cagagctttt cgtccgaagg agccatcgtc tgtaaccaca atatattcat
     3421 ccgcaattgc tctgagctcg tcctgcatta tgagaagatc ctttgttcga gcccccgaaa
     3481 tgaccgtaac cctatttccc gcttgcctta gagctttagc aatggggtat aatggagctg
     3541 atcctatgcc gccgcccaca catactgtcc agccataaca ttcgatatgg gttggatttc
     3601 ccagaggacc gagcaggata atcttgtctg ccggattctt gagggcaagc ttgtgcgtgc
     3661 tggcaccaac ggtctggaag atcagagtca ctgttccttt atctggatct gcgtctgcga
     3721 tagtaagtgg aattcgttcc gaatactcat catcgcacat aacaatgaca aactgccccg
     3781 gcttccgttc tcgtgcaaca agaggtgcct ctacttcgat tcggaagacc tcttccgaaa
     3841 gctgtacttt tgaaactaca ttatgcatgc ttctcattct caactccgca tgtattttag
     3901 cggggattag tgatttttgt ccataaacca gattattctc tctgtgctta ttccttctat
     3961 aatctccgct ttttgcagct ataccttgaa tatacctata caatactcca tcttaagaag
     4021 agaatagtac aatataagac cgattataag aagaagaaga aggagtaccg atgcccctgc
     4081 caatcgtatc ccaaaagctt aaagacgaag ctctattcaa acctctcgat tttctggctt
     4141 acctggataa ggccggtatt gccctatccc aaaggccggc gccccaaacc atcgtcctaa
     4201 gttaccagaa aagtctctgc gattttgtcc gcgccacatt tccttgtgag cgagccaaag
     4261 gctattttgg ccgcttcgtg cattatgtag atgtatcaga tatggatgat tcacgagata
     4321 agcactcgcc gatcggcaat aagaccatcg ctatcgcatc agacttcggt gtcggagcac
     4381 cggctgccgc agtcatgtta gaagaactca tagcatgggg agtaaaggaa ttcatctcta
     4441 ttggatttgc cggttctttg cgcgaggatg ttccgcctgg aacactagtg gtctgcgatg
     4501 gcgcattccg cgacgaggga acttcctacc actatctgga cagcaatatg tcggtacacc
     4561 ccgagccgag actcacagag gcacttgaaa actcgctcaa gagagctgga ctagcatatg
     4621 ccaaaggccc aacatggacc actgacgcca tttaccgcga gactaagtca gaagtccagt
     4681 acttccgcga ccaaggagcc cttgtggcgg atatggaagc ggcggcactc tttgcggtag
     4741 cgcagtatcg caatgtgtca atagcttctt gtttttctgt atcagataca ttggcttatc
     4801 tcgaatggag gcctgagttt cacgctgaac caagcatgca agggctcgaa atgctgttct
     4861 cttgtgccat aaaggcgtta caataatgtt gttcagctaa tgcaataaca ccgggcaata
     4921 tggaaaattc atatttagat gtctatccga ctctctagcg gccatgtgcc ggatacaaga
     4981 gacatgggca taacgacact tctctctccc aggatggtgc ctggattcaa taccgaattg
     5041 cagccaattt cgcaattatc acctataagt gcgccaaatt tggtaagctc tgtttcaaac
     5101 ttttctcgag aatctagaca ttttgcccta atgggctttc catcgagtct aaagttcgac
     5161 agaatgactc ctgctcctat gtgcgcatgg tgccccagca cagaatctcc tacatagttg
     5221 aagtgtggtg cctgcgctct ttccaggaga atgcaatgct tgaattcgct cgcatgcccc
     5281 aacacaacgc ctgaaccagc gagcaatccg cctcgaatgt agcagtggct tcggatttca
     5341 caaccctttc caatccaggc tggtccgcgg ataaaagccc cctcttctat aatgcaaccc
     5401 tcgtctatga aaacatcacc ttcaatgtgt acgccttttg gaatagagga tcggattttt
     5461 tctccagata cggcatcgag tctagatcga agataagaat caagtaactt gagcacatcc
     5521 cacggcatgg cggcttgcat gaacagatct tcaaattcgg cacattctcc gtattcatta
     5581 tctgcaaagt gccatagtct gggaaatgac ttgattttcc aactaccaaa aagaggcgat
     5641 ggataaaccc cttctgctac gaacccgaag atccgccgcc ttacttcttg caaatcttcc
     5701 gggtttgtaa ggccaaaact ccgctcgctc gcaggaagca tgcgtactcg tgctctcttt
     5761 ttctccacta tactctggat cagatcaggc aacaggaatt cctgcgtagg agattcgata
     5821 tatcgactca gaaattcctt ccagccttgc catgcatatt cgaaaatcga ataatccaag
     5881 ccccaaaagt tcatggagac agcagcatcg ggactcagct caactatgct ctccccctgt
     5941 tcggaaaaaa agcgctttcc ttcacgccat accttcttgt gttctattat cttttcaaga
     6001 taaccatcgg ctgataaggt acatattgct cgcgataccg ttccagaagc tggaacgaca
     6061 tcttctaatc gatacccagc gaagcataat tctcctggat tattaaatag atttgagcct
     6121 actgcctcga atgcacgccg gccatagtaa tcgtcagcat tgataaccgc aaaagcctca
     6181 ccttgtttga aaagctctcc agcacagaga agcgcgtgag ccgttcccca gggtttgaca
     6241 cgcttgctct ctgcaaggcc accttccctt tggcctaatt cccttgggtc tagctgcttc
     6301 tctccattga tctcggcaag agccttatcc aataggaagt cgagtgtctg ataggcgagc
     6361 gaatagcgta gatccgaggg taagcgcgaa aggatattcg atctgaaatc ggcctcgatg
     6421 tctggccgaa tcagaaatat aatttcgtcg aagcctgctt ttcgagcatc atatatggag
     6481 tattcgagta aggtctctcc attaggacca acctctataa tctgctttgc tccaccgaat
     6541 ctggagccga taccggccgc aagtaccaat agtttcatgg ctgaatgatg cgcattctga
     6601 tggccctggt caagccgcaa atactgctac gaaccttgtt tatggcagca aggattagtt
     6661 gacaagaatc agttgactgc catagaaaaa ccgacagtgt ccgagtatat tcccgcttta
     6721 gcaccatgat caccaggtat cttgatagtg cccaataagg ccttgctata aagacaatta
     6781 gcggtctctt gatcgagaat tatgcttgaa ttaagataat actctttgcc atcgactatt
     6841 atcgtgtatt gtatcttgtc ttcagaattg ctggaggtaa gaaatccacc ggaggccgta
     6901 tgcattgtta gggtatatcc agttgttccc ctgatgaaaa tcccaaaatc ggcgcttgcg
     6961 ccatttgata tctcgccaag atcaatattg tatgaagtcg ctccggtatc ataattggca
     7021 ttccgcgaac caacgcgcac atctactgct ttgtcgactg ctgctgtgta agtgaaactt
     7081 atagtatctt ccggattgcc ttgtcctggg gaactctttt tataaagtaa tagagtaaaa
     7141 gtcccggtat aggtaccata tggtgcgctt tggcctttga caaggcgagg atatattgta
     7201 aaggtttgag taagaacatc tggctttgga tcgaaggatc ctgagatata ataagaaggc
     7261 gaataatctg cagtgcttct gatctctgtt gtatgagagg aatctttgta gaagcctatt
     7321 tctatagacc ccgaagatga tttaagactt cttgaagagg gagacgattc tccatttata
     7381 acaactgaaa aattgcttgt agtgcctgtg cttttatcca ttcgcaccgt aaaggtaaac
     7441 gcctggccac gagtgtcgtt cccagaaaaa ggatcataag taacaggact cggagcagat
     7501 atcggatcta aggtcaattt ttccgcactc atagaaatga cgatagcgaa acaaattatc
     7561 aaaaataacc aaaatcttct tctcatatgc tattccttta ttcattatag tcctatttgc
     7621 tctcgatgtg tagctttata tcacctaagt ctattctatc ctcgtcggtg tcgggcaatt
     7681 cgaactgagt ggttccaaag ccttctggcc attgtatttg gtaacttccg ggaagaatat
     7741 catagatctc aaaatccccc agttcatcag taaaggtaga agtcacaagc gagcctccaa
     7801 aatcgaagac atcacccggc agatatgaaa ttggtttgcc ttcttgattg ataagtctac
     7861 cgttaatgaa atatcgtttg ataacatcgc tttcgaatag aataccactc ctgaaagcag
     7921 gggagagcgt aataaaggag tagcgcggag caacattcag ctcagtggct ctaagatcga
     7981 tcgatagtat tgttgtttcg tagctttgaa taggaacgac gatgtttttt ccgcctgctg
     8041 cctgatactg agatccattg ctgactctat agacaatatc cttgtctttc aattctggcg
     8101 aagccgtaat caggaggaaa gaatccggta tctggcgcaa aaacgcaaca tgagtcccag
     8161 caaaagccat cgagcttcta aattgggtgt atccacccag agaatagctc tccgagctag
     8221 gggaatagtt gaaggttcca tttgcgctta catcgaaata ttctgatgaa tttcgtattc
     8281 ccagactcat cgagccatct ttcgatgcac caggaagtaa gtcgcttgcg ccaaaattcg
     8341 tatcaaaaac tctgccaagc atgtagagct tatcgttgat gctgatattc gtctgtcctc
     8401 cggaactctg catgaaatta agaactctcg ttgtgtcgtc tttgggcatt atcgtgaaag
     8461 acaaatttag tgaaaacgtt ggtgcactag attctgcgaa agaaaggcgt cctgaaagac
     8521 caagatttcc gccagccgca attgcggtgc tgattgaagc aaagacttca tgagtcattt
     8581 tagcaggcga tgagtatgta taataaaggg aatagctcaa tcctatgcca atatttgcaa
     8641 agatcttcgt gtagaaattg gtcgagaaag agagaaaagc actgggaacc gttccgctgg
     8701 gagaagatat attaggagat aagaatcctt ctcctcgaaa tgtggcagag attccaaaga
     8761 tgggtaggta tatctttgaa ggcatggaaa agagatagct cagcgttgcc gctggtgaaa
     8821 taacaggtcc ccagccatcc catcgcccta caatagagcc ggaaccattc agtgtgccga
     8881 ttggcagtgc tgtagaaaaa gtcagtccac ccatcgcgga gcgcttgtcg acctgaaagt
     8941 ttatgccgcc cgaaagagtg gacaaaaagc catatagata ataacctgag gcgataaatt
     9001 cgctgaagtc ggtacgcgat gttccagcgg aaagagcata cttcgatgtc ccgggttgca
     9061 ttatactggc ttctctcgga attatgcgac gaaatacctg gacatttcca tctatctctt
     9121 ctatgcgcag aacaaattca ttcaaccctg ttgtaaaggg cagatcgaga atccgataat
     9181 tgccaggttc gagaataact tgccgaataa gccttccatt agcttctaca gacacccttg
     9241 cagtcttatg gatcgtaaac tccgtttgat tgtctatgta tccagtgcgt accatatatt
     9301 gtctatcttc gaagctttcg agagacattg cataaagcga tggttgagat tgattgctca
     9361 ctgccgggat gctgatcttt ccaattttcg tccttgaatg tattgctgtg ttatcccaaa
     9421 gcaaataaac cgagttcatg ttccaggaaa aaacctgaga aggcccgaac gcatatgaaa
     9481 aaaaattgtc tgagaagaga tgaaccccct ttacgtcgat catcgagttg agacgtgtag
     9541 aaaataggac ttttccttcc gatatatcag agacatgcaa gtccgattca ttctggatat
     9601 agcctgcaaa aggttcaggc cggagaatag gcttgtaatt caaaatttgt tcaggcacaa
     9661 agtcgatatc gaccacaggg ctgtattccg gcgggatcac aatcgaaaga ctaagttgca
     9721 attcattaaa taaaaaggaa ataccagcaa gagaaaaatc attggccgtg agccaatcga
     9781 gctctttgaa tagcgtgtcg aatataaccg aatatatatc gggacgaagg taatttatga
     9841 gaagccgcga ggctatttct ttattgagaa taggttcatt gtgccatatt cctgtttcta
     9901 tatctcctat cggtttgcca tttatactga tggagaattg tatctccagt tctggcacaa
     9961 tgctatcgtg aacgataggt gtttggggga tttgcgcatc tatagggggg agccataaaa
    10021 acaaaatcca aacaagaacg tatacatttt ttttcttcaa catttcttgc tcactctggc
    10081 cttacaaaaa gccttgaggc tcagcgcttc caaagatcat gtagaactgc ataagagatt
    10141 cattcgattg ttgcggtcag cgtgcctgta tagtttttgc caggaaccgc gtcctcgcag
    10201 ggaataaaga attttcgcac attgccagcc agcaggtttt gaccattgat tgcactgaca
    10261 gattcatctt tcagcgtgat tgtaaaagag gagccagaga tcttcattac aggatcgttg
    10321 ataatagcat gacgcgtacc actattggtt attgtaacaa agaaaccctt tttgctttct
    10381 tgctctccat actcaacctt tgtgacactt accttatggc taacatttgg cggggctaca
    10441 taaagagccg caatgtatcg aaacatcacc ttgacgccgg aagtctgctg ctggctaaag
    10501 tcgatgggta cctgctccgc aataatgcga taagcgactt ctcttgaaag cgatgggtta
    10561 cccttgtatt gtaccttgat attctgaaag gagttaggct gtacgacaac gcgagtcggg
    10621 aaaatagcga atagattatc tgcgggctta ttggtttcgt tgccattctc gtcgatttct
    10681 cgcatcatag ctttaatggt tatcgcgatc tgctgcgaac tgtcatttgt aattcgaaaa
    10741 ctcgcgatac tgtttgcacc gctgggcgat atggttatcg acattggcat gaaagtaaat
    10801 gctgttgccg ttgccgcgat tgagattaga aagaccgcga ccagaatgag cttacgacaa
    10861 gactgtctct tatacatgca ttcctcccac tatttttatt attctatatg taaaagagga
    10921 taagtgcaat tgcattccga gatcgcttga caatctctct atctgctggt ataagagctt
    10981 ctgtcgttcg cggcatgcag cgggcggtta gctcagttgg ttagagcacc agtatgacac
    11041 gctgggggtc gcaagttcga ctcttgcacc gcccaatgcc tttatatgat aggccgctgt
    11101 agctcagttg gtagagcaat ggactgaaaa tccatgtgtc atcagttcga ttctgatcgg
    11161 cggcatatag ccacctcaaa atgaggtggc tttttttatg cattcgaacc aggaggctca
    11221 aacaggatgc taacgacagg acgcatcgag attggcttgg tcatccattc tattgccaga
    11281 ctcccatgat aatccatccc atcacaatag gctcaggttt ctgattttcc gatagggtac
    11341 tgcggcaaga atcttctgta ctttttccac aagttccgga tcttcgtcgg agagaggcag
    11401 ggtgagttcg tttttcttta gaattctgtc taaatcggta taggataggc ctatggcaaa
    11461 ttcatcagtt atgccagggg ctagatcagg gctgggttct ttttcgatga ttctctgtgg
    11521 gaggccgatc tctcttgcca gctcgaatac ctgtgtctta tagagatgca ttattggttc
    11581 tatatcgctc gaatcatctc cccacttgac atagaatcca ataagaaatt ctgttctgtt
    11641 gatggttccg gcaaccgcat aacctctctg ttctgcttca taatagagaa ttgtcattct
    11701 caatctgttt ttggttctat agtatgccag atttcttctg ttggattgtc cgcctgcgct
    11761 tttaagatcg ttgatataag gagagctggt cttttgccat tgggctctaa catagtcctc
    11821 ttgaattttc cttggaaata taaaagctgg gggctttgtc ctatagattc cgagacgtct
    11881 aaccagggga gaaatgctct ttacagaata tctaattccg agatatctac agaccaacag
    11941 ggaatccgag actgttctct ttgatgaatc tcgttccggt aaaatgagag caaagactct
    12001 ttcccgacca agggcttccg tcaagagttt accgacaaca gcggaatcga tccctccgct
    12061 tatgcctatc acggctcctt cgaattcata cttggcgatc tgcgtcctga tgaattcaac
    12121 gattctttcc ttttcttgag atggattgat ctctgcgggc atgaagggcc tctttgatat
    12181 cagcgaaaat acacttattg atacatcgaa gaatatgttg ccatattgac gccgccgata
    12241 tattcaaata tagcataaat gctgtagaga ttcgccaagg agagaaatcg catgaagata
    12301 ctagtgatcg gtggagcagg ttacattggg agtcatgttg caagaatgct tgctgacaat
    12361 ggcaataagg tcagcatcgc agataacctt tctaccggca cagaagagaa tctcttttct
    12421 gattatgatt tctataaggg cgatattctg gattatccct ggcttgtttc gataatgaag
    12481 catggattcg acgcaatagt acatcttgcc gccgcaaagg ctgcaggcga atccatgatc
    12541 aagccagaaa aatacgcgac acaaaacatt accggaacga taaatatcct caatgctgca
    12601 tgtcaagcgg gtatttccaa aatagtattt tcttcctcgg cagccatata tggtgagccg
    12661 aaatacctgc cgatagacga agcgcattcc aaagaaccaa ctaatttcta tggttttact
    12721 aagctcgaaa tcgagcgtct tctcgaatgg tatgataagc taagagggat caaattcgca
    12781 gcgctgcgct atttcaatgc ggctggatac gatattgaag gcagagtgcg aggattagag
    12841 aaaaatccag ctaatctcgt gcccgtcatt atggaagtgg ctgctggaat gcggtcaaag
    12901 ctcgaaatct tcggcaccga ttatcctacc ccggatggta ctggtgtgcg agattatatc
    12961 catgtgacgg atcttgccca agcccattgt aaggctttga atcatctggc ggatgcaagg
    13021 caaagctttg ctgtaaacct gggatcggaa aaaggcctgt ccgtcctgga aattcttaaa
    13081 gccgcaaggc gaatcacggg tcggccaatt ccggcttcga tagtggagcg gaggccagga
    13141 gatcctgcta aactgatcgc atcgagctgt gcggcaaaac agctcttggg atgggaggcg
    13201 aaatattccg atgtagatac aattatagcc acgtcgtggc gcgtctacga aaagcatttg
    13261 gcataggcaa ttcgatagaa gcatatctgc ctggaaagga aaggggaaaa tcatgacaga
    13321 acttgctcga gcatttgagt ttctggaggc tcaagagagg gatattatcc aattagagag
    13381 tcttctcact gcttatccag cgctcgctcc cgaatcagag ggaattggag aaattgagaa
    13441 agcttctgct ctggaagcgt ggctcaaggc acaaggaatc tcgaatatcg agcatttcga
    13501 tgcgcctgat ccgcgcgttc cttcgaagtt acgtccaaat ctggtggcga ctatccctgg
    13561 aaggcgtgta gaaggtccac tctggatcat gtctcacctg gatgtcgtac cggagggtga
    13621 actaagctca tgggagagcg acccatggaa ggtagttgtc aaagatggca agctctatgg
    13681 caggggtgtc gaggacaatc agcagggcat tgtatcatca gtatttgcgg cgctggcatt
    13741 cgtaaagcag ggtattatcc cggagagaac gataaagctc ttattcatag ccgatgaaga
    13801 ggtcggttcc aaatttggca ttcaatacct tcttgagaac catagcctct ttggaaagga
    13861 tgatattata gtcgttccgg acggcggctc ggccgatgga acagaaatcg aagtagccga
    13921 gaagaatatt tgctggctca agataaggac caaggggaaa cagacccatg ccgcaatgcc
    13981 ggacaaaggc gcgaatgcac ttctcgccgc ttgtgatcta gctctacgcg tgcacaggct
    14041 ggagaaatcc acttttaccg ccagagatcc gcttttcttc ccggatcgta ccaccataaa
    14101 tcccaccaaa aaagaagcaa atgtgcccaa tatcaacaca attccgggcg aggacgtttt
    14161 ctatttcgat atgcgtattt tgcccgtcta tccggtgtcc aggatgctcg aagaggttat
    14221 gaagcaggcg catgctatag aagccgaata caatgtaaaa atcgaactcg aagtgattca
    14281 atcgaatgaa tcgaaggcta cgccagcaga tgctcccgta gtaggtatgc tggctgaggc
    14341 tgtaaagcag gtatatggag tcgaagcaag accaattggc ataggaggtg gaacggtagg
    14401 cgcttatctg cgtaatgctg gtttagattg cgtagtatgg tccaagatga acgaaactgc
    14461 gcatcaaccc aacgaaaatg cggatattgc gaatatcata ggcgatgcaa aggtctttgc
    14521 ggcgctagca atggctcctt agagaatcac aggcgaaccg gtatctttag tctctggccg
    14581 attctcaaaa tcgaattcaa tgaaatgcca ttgagattag ctaaatcctt ggccgtagtt
    14641 ccgtatcgac gcgctagagc gtagagagta tcaccttttt gtacagtgtg aattgaagtc
    14701 tgtatatttg cgcccgaatt agcaatgttg cttgagctgc cagaagactg aaggggctgt
    14761 atggataaag gaatcattaa ggtttgccct attcttatct tatctggatt gatgcctgga
    14821 ttgacctttg ttatcattga aatggagata ccatagcgct ttgaaatggc tgtaagggtg
    14881 tcgccttgct taaccttgta tatttcgtat ctcacaagag gtgcgtttga gtccgctaga
    14941 atggtcttta cttgatctga cttatcggct ggtatcttga gaagatgatt gttctctggc
    15001 ggcgtgattg tatagtgcaa ttcagcattt ccaagcttaa gagtatcgag cggaatttcg
    15061 gcctttgtag caagaagctt tatatccacc tgttgctcca gagaaatggt ttcccactta
    15121 attccggacg atggtagctc aattccatag agatcgggat agcgcagaat cgaagcgacg
    15181 gccagaaatt taggcacata atccagagcc tgacgtgaaa ccaatccctt attgtagatt
    15241 gtccagaaat cacagcccgg tcctgcggta ttaacagcgc gcctcaacga tcctaagcct
    15301 gcgttatatg cagctatagc aagcggccag tccttgagtt ccttgtaatt atccatgagc
    15361 tttttcattg ctgcatccgt gctcttcata aaatcgcggc gctcatctac ccactcggtt
    15421 attgtgagtc catagccatt tatagaattc ttcatgaact gccagatacc cgttgcaccg
    15481 cttttcgata ccgcaaaggg cgagaatgcg gattctataa caggcagaaa ggccagctct
    15541 tgaggaacgc catattctgt taccttggat tgaatataat ccataaatgg cacggccttt
    15601 tccatggtag aaagcagaat tttgcgaccc tgattactca agtaatactg acgatatttc
    15661 tcgaaaagct cgtgacccca aggcatggga atatcatatt gaaggctttt ggctctctct
    15721 tctatgctga gctcttcgat gctcggctcc tctggcagaa attggctaaa atcgagagac
    15781 tcttgtgttt cttcgatggg ggtgctctct aattcttccg gcggctcaag tatagatgag
    15841 acgcctacaa tatattcatc ggcttcttga aaagaattag cgccttcgat ttcttttgca
    15901 gatgcaaagg acagtgcggc gtataacata cacaatattc cgatagactt gattttcctc
    15961 attgcatttt ctctccgaag tagataccag cccattccca gttgccatgc acattaggat
    16021 ctgctggaaa gttgactttt cggaagcaca gataccacac gggaatgtag cctgtccatc
    16081 ctgatgtgcg gagaagagct tccgagtcgt tgaaagatgg atcgaaggca ggagaaacat
    16141 atatggggcc cctggtcata aagttgctaa aattaaaaag gacattagaa ttggattctt
    16201 ctcctccttc cttataccag gacatagcga agaatcccgc cttggacatg tattttttga
    16261 gctctgaggc tgagcgcttt gcaatcgcct tgcgaatacg gcttacaaga tcgtttagat
    16321 cggcgtatgt ccaatccttt gggctatcgt tgaattcgac cattttgcgc gcataatcat
    16381 gggcgtccgg atatccagga acagatacac caaaaaaagg taagaattgc cgatattctt
    16441 cgatagcttg cggccaaagc cctaattttt cgtactcctt tgcaagaaga aaatggtaag
    16501 ggcccacttc atcggcctcg ggaaagcgtc ttatgaattc tccgtaccaa tgcgcttttt
    16561 tatacggcgg actatcgccc tcgagcaagc ttatgagaga tatccgatgt atcgactggc
    16621 catctatcat catatcgggc agattcttga gaattctctc atatagaatg cgggcaatag
    16681 gatcgctatc catggactcg tagactgcac ctgccgcaaa gagataccaa gcttcataat
    16741 catcatgctc atctgccaga gaagaaagaa atcttgcagc acttgctttc tgcccatctt
    16801 tgatcagaat ggtgccgatt cgtttggctg caagcatgcg ctggatcgag taatagccac
    16861 tattcttcaa ctgaggagaa taaaagattt caagagcgca gcgaagctct cttcgaagag
    16921 atctattcga tataggaaat tcgcccaaat tggaaaaacc gatccccgaa atctgaagac
    16981 cccataaccc caagcaaaag acgatcaaaa tcgaaatgag gaccggagcc gcgaatgcac
    17041 ctatcctggc acttttcatt cttgatacat taccatgcat cattttttct gggcaagcgt
    17101 tggtcagagg aatcataggc acaaaatgga tcaagccatt ctggataccg cgcggataaa
    17161 gcggataaat tcaccaaatg atcgatcaac gtcatttttt ttcttgcagt gcgagatcat
    17221 ttaggctaga ataataattc tccatgaagt gccgatatag atattggatg aaacgcaact
    17281 ctatatttct ccttgcgata gcagtgtttt cagtattgtc ctttttagcg ccttcttgga
    17341 gctatgcaca gcaaactctg ccaaagaccg atgtagaaca gcttaagaaa atcgcaagtg
    17401 aggccacaga tatcgagaca ctcattgtgc ttgcgactcc tcgatcctta tctcaggcgc
    17461 gctctaggac caaggcttcc ccttatctat cggaaccaaa caaagcagca ttggatgcaa
    17521 ttgcaaagag catcggcctc attttgtatg ggccaaaaaa tgcgcctttc gagattcctg
    17581 tgttagcaaa aggagccgat caaaagtata tcacatgttt ggccggacta tccgacgctt
    17641 cagcaggaaa gagtccaaag atttttgaga atggaagttg ttcgctgatg ggcgagctca
    17701 tctctgcttt accattcttt cgggattctc gcaacgaaat tagagcggga gcgcttgcgg
    17761 ccatttccag actcgaggat ctgggtggta tttcgaccgt tccgccactt gtgagaggtc
    17821 agatagctct cgaagcaaag caatacaccg aagctgccag tcaattccaa aaagcgctag
    17881 aactcgatgc gctctcagag aaaccagcct gtggccttgc gcgtgcatgg atcgcgctgg
    17941 gagagcctgg aaaagcaaag caggttctcg atcccataat atcgattgct ctggctgaat
    18001 ccgctaaaga aatatcggcc aaagatggat ataacgaaga tccttcttca aacaaagtgg
    18061 cctccagcgg aggaggatat atagaaacaa cagaagcaga gcaaaatatc ctgagcgccg
    18121 atattaaatc attatatgga cttgcattat atcatctgaa taacatactc gatgccgaac
    18181 cctggctcaa agccgcactc aaggaagatg catccaggac ggatcttctt gttcccctag
    18241 cccatgccgc attgcaaagg cgagactatg cctcggcttg gaagaatctg gaaggtgctt
    18301 ccaagattgc ttcaaaggac agaaactggc tcatcctcaa aagccaatat gctttggaga
    18361 attcgcgtca agtcgatgcc gagcgctttg ccaggaccgc agtgagtcag tacccccagg
    18421 atcctgttgc gcttgctcag cttgttagag ccattcagga gtcgaccgat gcgcagcgac
    18481 ataaggaagc caccgatttg gcaaaaaaag tgctggatat tacaaagaat gaacctccta
    18541 atctaacacc gcttgagcaa gcatggagga aacaagcgga ggacaaggcc ttccagtttc
    18601 tcgtaatgga gaactataat aatcaggatt ggattatggc agcgaaatat ctccaggaag
    18661 cggggggtgt tcccctcgat aaggagatgg tagccactat tctgagaaag tcaggaaatg
    18721 tccaggaagc gttacagttc gctttgagct ggtatataca agaacctact tccgaaaaag
    18781 ccgttgaggc ctatttgcgc agccttgcta tggcgacagg ggcgactcta gcctcggccg
    18841 caccgaccag agacgttcct acggctattt tgccggggat gtccgggctt gagatgagac
    18901 aagaagaagg cgcgaatttg gctctgctcg atatagtttt gaaattcata gccgcgcctt
    18961 tttcaaaaga gctaaaatct ttcctttatt atatgtctgc cactcttcag agcgatgaaa
    19021 acaaagctat agatcagcta aaagatgcgc tcgctgaaag agctgacaat gtggaagcac
    19081 ttgtctcgct cgcttcaata tacttgaatc gctataatcg acaatccgac aagtccgata
    19141 caataaatcg agataaggct gttctatacc tcgaacaagc gaaatctctg cgtccgaacg
    19201 atgatgagat taaagcccgc ataaatcagc tagaagcaga gctcggttat tcgtctagat
    19261 agagacagag ctagagcatc gatatggcga caatgtcgaa aacaaaatca tgaaaaacct
    19321 ctctctacaa aaatccgaaa agtccagccc acaaaaggac aacgaaagaa tagctagccg
    19381 gcaagcacat agcccccaaa aagtcagctg gcaaaagccc tcctatgacc cagcaatttc
    19441 atgttttctc gaagagatct atgcggaatg caatagatcc gagcgccttg agctcgatcc
    19501 gcttgccatt gtgagacgct atgagaagcc ggaagatatg gaagtggccg gactcttatg
    19561 ttccacgcta gcatttggat cggtagagct tatcatgcaa gcctgcgaaa aagcgctcaa
    19621 gccactggga gagcatccag caaagactct gcgcgagatg agacacaatg agatagagca
    19681 agcgtgggcc ttttttcagt atcgtttctg ttttccaaag gatatgatcg ccctcatgtc
    19741 ggccattcat gaggcattgg atagctatgg aagcctggaa gcgctctttc taaaatgcga
    19801 tacactatcg cggcaaaaca cagggaaaga ccctggcgat tcggctgcaa atgagcaaaa
    19861 aacccacaaa ggcaagaccg agacagattg gcaggaaaac attttggccg cctcatcaac
    19921 ttttgttcta aaattgcatg agctggcgct caaagctcag cccgaaggct tgcgcaagaa
    19981 tcttctgccc gatccatgca aagggtcagc ggcaaagagg ctttttttgt ttctgcgctg
    20041 gatgataagg cacgatgaaa tcgatccagg atgctggaat agcattagtc ccgcacgcct
    20101 catagttcca atggatacgc acatgatccg cacttgcaca gagcggctta gattcttgcc
    20161 gcggagaaaa agcgctggga ccatggcgag ccttcgagac gcgctaagcg ttaccgaggc
    20221 ttttcgactt tatgcacccg aagatccggt caaatatgac tttgccttga cacgtccggg
    20281 tatagatccg cgccccggcg atgagcgctt ctcttgtcta tgagcccaat cgatgagcca
    20341 agctacaaaa caaaaacata tcaaaactgc gccaattgca gccttcagtg ccaattggag
    20401 cctcctgtac caacaacggc ctcctgaaag gctcggattg cgcaccttat ggaggaacag
    20461 ttttcacatt gacaacgcta ggcggcttgt gttagtttat ttgagccccg acaggcaaat
    20521 cgggcaatag cgcaggtggt tagcgtacaa gtctggggga cttggggtcg ccagttcgag
    20581 tctggcttgc ccgaagagga gcaagttgtt gcaatgcaat gacttgctct tttttttctt
    20641 atacacaagg ttcgtagcag tattgacaga ataccctttg tagcatacat catgtaaatc
    20701 ggtttagtta tgaaaatacc aagaagcggc ggcgtgctcc tccatcctat ttctctgggc
    20761 agcccatggc cttgcgggac catgggtgag gaggcgcgtt tctttgtgga cttttgtgta
    20821 aaatcgggtc tcggatggtg gcaggttctt ccattaggcc ctacggcatt cggagattcg
    20881 ccctaccaaa gttcatcggc atttgcggga aatccttact tgatcgatcc acaagagctc
    20941 tgggctaagg gattgttatc tagcaaggaa attgaatcct atcgctggaa aaaagggaac
    21001 aaaagccggc cttcttttgc tagccctgaa gcagagagca gtatcttgtc gagcttgcct
    21061 gaagatctac aatatgtgga ttatgggcgg ctttttacat cgaatgcaag gctcatggat
    21121 gcagcctggt tacgctatca agccaaagcc agggatgaag aagcggcagc cttcgatgaa
    21181 ttctgtaaag caaatatgtt ctggctcgat gattatgcac tttttgcagc aatcaaagaa
    21241 aaagaacaac accgaccatg gtatgaatgg ccacaagaat tcaagtctag agatcctaat
    21301 gctcttgctc gattcgaaaa aggcaataaa gagcttattt cccgcttaaa actcacccag
    21361 ttcctattct ttgagcagtg gatagctctg aagcgatatg ccaacagaaa gggtttgaaa
    21421 ctgataggcg atgttcccat ctttgccgct tgggattcag cggatgtttg ggcgcatcgc
    21481 gagctcttcc aactcgatga tatgggtaga ccaatcaggg tagcgggtgt tcctcccgat
    21541 tattttacgg caactggtca gctatgggga aatccgcttt atgattggca agcgcacgag
    21601 cgagatgctt atgcatggtg gatagaacgc gtgcggcatt tacttaagct cgtggatgcc
    21661 cttagaatag atcactttcg aggattcgag gcatattggg ctgttccatt tgaagaaccc
    21721 actgcgaagc atgggcgatg ggaaagaggc ccagggcata gactcttcga tgcgctcaaa
    21781 agcgcactag gagaattgcc cataattgcg gaggatttgg ggttcatcac tcctgaggtc
    21841 gtcgaactgc gcgatagtct gggttttccg ggcatgagaa ttttgcagtt tgcctttgag
    21901 ttggaagaag acaatccgga ttatccccat aattatcctc aacactgcat tgcctacacc
    21961 ggcactcacg acaacgacac cgccagaggc tggctaactc atgcacctgt ggccgcaaaa
    22021 aaacgcgcgc tttcgtatat gcacgcaaaa cccagcacat tccaccagga catggtacgt
    22081 actgtgtggg caagcccggc tatgctcgct atcgctccaa tgcaggattt tcttggcctt
    22141 ggtacagaag cgcgcatgaa ttttccagga accacgagcg gctggtggcg ttggcgcatg
    22201 aacagggaag atctaagccc agcgctcgcc cggcagatac aaaggctttc cgaaatctat
    22261 ttcagaacgg acgcctcgga ctgaaaaagc agagacagcg cagtgtacgg agactcaatg
    22321 aaaggcattc agcttttcga tggagatatt gtcgtcttta tacca
//
LOCUS       DAOMZL010000025        22169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_2019_length_22169_cov_16.142625, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000025 DAOMZL010000000
VERSION     DAOMZL010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 22169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22169
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2019_length_22169_cov_16.142625"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..829
                     /locus_tag="PLQ30_03545"
     CDS             266..829
                     /locus_tag="PLQ30_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01548.1"
                     /translation="MQNPFYVPLSRKSAFLFGIVVFCLIAPFAFAQENQTSQPDKNDL
                     LRVSLSASLVVTNTDEKGVVRETLQPLPDKVYPGDVIQYSITAINTSTGCPCTDMLKN
                     IALIGTINKGTVYIEESVTLPPLAMFSIDGGVSYHPWPISYIVETADGSETVKTATAE
                     QYTNIQWIIPPLGPQERLTVSYRVRVE"
     gene            907..1317
                     /locus_tag="PLQ30_03550"
     CDS             907..1317
                     /locus_tag="PLQ30_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPW01549.1"
                     /translation="MKRKASFLICLSLLLAVMTSWSENGEAQSIYPESVYELLASKQD
                     IILIDVRTHDEYVAGHIPGAILFPVADINSESAAKIIGPDVTRMVIAYCASGNRSRTA
                     TSLLLDLGYMNVWNMGSLSAWPFEIEHGEPLLVP"
     gene            1424..>2283
                     /locus_tag="PLQ30_03555"
     CDS             1424..>2283
                     /locus_tag="PLQ30_03555"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HPW01550.1"
                     /translation="MLLRKHDRARYGERVMINVPALAKILVIFALIVIAASKHVHLGL
                     AAAVGGILIALWQGMNTEGIVNAALMELTNPDLILLVLLLAAIMTFSEAMKISGAMNV
                     FSNTVAKAMPSRRIALGVVPLLIGTLPMPGGAIISAPLVGALNDDDARGPEALSAVNY
                     WFRHILELAWPLFPAFILTSGLTKLPTLTLIGLNIYAPIVLLILGFLFVLPKSRNNSE
                     GAHGSADAIPSLPNAIPLSMATPSSCGRASADSLSGNSISIGEKPSIAEVLHGIAPLG
                     LVLGTYIVIDV"
     assembly_gap    2284..2383
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2384..2713
                     /locus_tag="PLQ30_03560"
     CDS             <2384..2713
                     /locus_tag="PLQ30_03560"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HPW01551.1"
                     /translation="GIPALVVIAVLPFISGIVTGVGMGYVGLSMPIVLALIASSGIPL
                     KAGVMIANAFGYTGMMLSPLHVCMVVTAQHFKSSLIKVILKCALPLAIFLIIVIAYGA
                     LLMALMR"
     gene            3154..4170
                     /locus_tag="PLQ30_03565"
     CDS             3154..4170
                     /locus_tag="PLQ30_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPW01552.1"
                     /translation="MNQSPLNEEKSAPSLSGMAMLILDVFLMIVTVAGFVFGTSSGKT
                     DAGTIVLMVVCGLYAFLIGPILFAGLKVLKPNEAMVLTLFGNYHGTLRGPGFFFVNPF
                     VASVCPSNVQLSSGSIPLPIGETGSSSAAHKTHGASSKQASKKISLKAMTLNNEKQKV
                     NDALGNPVIISIVVIWRVVNTAKAVFNVDNYFEFLSIQCDSAARNIVRLYPYDASEEE
                     GEKSLRGSCQEIAESLKEEVQKKVDMAGLEILEARITHLSYAPEIAAAMLQRQQASAI
                     IAARQMIVEGAVGMVEMALAKLSDRNIVNLDEERKATMVSNLLVVLCANKDPQPIVNT
                     GSIY"
     gene            4281..4454
                     /locus_tag="PLQ30_03570"
     CDS             4281..4454
                     /locus_tag="PLQ30_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HPW01553.1"
                     /translation="MEGLEKDNEKKQILLRLSPTLWKELAAWAADDFRSINGQIEYLL
                     TECVRQRKKKKIE"
     gene            4524..5381
                     /locus_tag="PLQ30_03575"
     CDS             4524..5381
                     /locus_tag="PLQ30_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW01554.1"
                     /translation="MEGLSFEAVPGEIFGLIGPNGAGKTTTIRMIMNIIVPDSGIIRF
                     NGAPLSEADKDHIGYLPEERGLYRKAKLGEVLEYLAAIKGAKANEYRPSINAWLERFG
                     LSEWKNRKISDLSKGMAQKAQFIATVAHNPSVVLFDEPFSGLDPMAQDDLLSVMVELK
                     HKGAIVLFSTHIMEHAEKICEKILLMNKGKAIVYGSMADIKAQYGRNAVQLEFDGDAS
                     FVQSLDFVDSVVSYPRWIEVELKEGTDSDLLFKALAGRLKVRRFETVAPSLHKIFIHL
                     VGGEEIENG"
     gene            5374..6615
                     /locus_tag="PLQ30_03580"
     CDS             5374..6615
                     /locus_tag="PLQ30_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW01555.1"
                     /translation="MGKLKHIIRHEFRLTTANKAFVILTIIGPFLIFAITVLPTLLAQ
                     SSSPVASDKPLALFGAPPRVEQAISAAFSGMKLQVEHVEDIEAAKASVIDGSRVALLE
                     LEAGWPDTGKAIWYSKSSAEIGLYSTASAVLEAVAREERIRESGIDPALAAKLLSKPT
                     FEVIKLEKGGEEKAQGQGDQAFLETLMTVLVFIMLLYMTILLYGQMIGRSVVTEKTSK
                     TVEIMLSSVTSRDLMIGKILGLGLAGMLQYAVWISMAFVLNHFLGPLFGLALPVAVSM
                     QNMMWLFVFFILGFFLYSSAYAALGAASEDEQHLGQIAWPLLMFLIIPMVMLNTLVTS
                     PDSTAVVIMSFFPLTSPIVMLSRILVASPPVWHLVLCIGILVASIVGMMVFAGKVFRV
                     GILMTGKRPKLKEVLRWVKVK"
     gene            complement(6624..9062)
                     /locus_tag="PLQ30_03585"
     CDS             complement(6624..9062)
                     /locus_tag="PLQ30_03585"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPW01556.1"
                     /translation="MTKFSVTGMTCAACSAHVEKAVAAIPGVKSVSVSLLTNSMSVEF
                     ASPASAESICAAVDKVGYGAAPEDVDSGLSAADAARKRASERDALDDHETPRLVKRLI
                     ASLILLVPLLYVSMGHLMWKWPLPAVFSENPAAIGLYELLLTLAIMMINQRFFTSGFK
                     SFVHGAPNMDTLVALGSGASVVYSVVVLFLITGAMLSGMLHDAHEYLHDLYFESAAMI
                     LTLITVGKTLEAYSKGKTTNAIKSLIDLAPDTAHVIRDGKELTIPAEQVRTGDTFIVR
                     PGERIPVDGKVISGTSAVNESALTGESLPVDKSAGSDVSAATLNQNGFLTCTATKVGS
                     DTTLSQIITMVENAAATKAPVARLADKVSGIFVPIVIVLSVITGIIWAVAGGEFGFVL
                     ARAVSVLVISCPCALGLATPVAIMVGSGLGAKHGILFKTAAALEAAGKTDIVVLDKTG
                     TVTEGKPTVTDVIPADGVTERELLALAGALEEKSEHPLARAVLEKVRAENIAYSPAED
                     FTALPGYGVKGVVPPSGNDSGAVAPVAGVPASGGNAALMKDQRLLGNEMKQRGMELAG
                     EGKTPLYFAAGGKMLGIIAVADVAKKDSADAVQKLNALGVQVVMLTGDNRRTAEAIAK
                     QVGITHVVSDLLPGGKDAVVAKLSAFGKVMMVGDGINDAPALTRADTGVAIGAGTDVA
                     LDAADVVLMKSTLVDVPAAIKLSRQVLTNIRENLFWAFIYNIIGIPVAAGALIPAFGI
                     VLNPMLGAAAMSLSSFCVVTNALRLNLFNMYKSGRNGKRKTIQLPDDIFDGRNISHNI
                     QNLPDNFVTQSPHV"
     gene            complement(9097..9438)
                     /locus_tag="PLQ30_03590"
     CDS             complement(9097..9438)
                     /locus_tag="PLQ30_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HPW01557.1"
                     /translation="MNEDAGGNVPQVADEGGCVSCRYKNTPREEQTLKQLQNRLNRII
                     GQLNGIKTMLDDNRYCGDVLTQVAAVESALQSLGYIILRNHLETCVVEEIKKDNMQIL
                     DEALELIRKLK"
     gene            10274..11377
                     /gene="glf"
                     /locus_tag="PLQ30_03595"
     CDS             10274..11377
                     /gene="glf"
                     /locus_tag="PLQ30_03595"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HPW01558.1"
                     /translation="MKYFEALVVGAGLAGSTAARILAEQGRKVLVVEKHRHIAGHCHD
                     ARNEAGITIHTYGPHIFHTNNKTVWDFVHRFTEFRYFQHRVLSYVDGRFIPFPIQADT
                     INELFGTSIATQEVSDFLAEEVRQSKFQTLPATFRDVVVSQVGERFYSLFFENYTRKQ
                     WGLDPTLLSAEVASRIPVRTNRDGRYFSDRYQGIPAHGYTAMVDEMLNHPNISVLLGL
                     DWFNIKDEIAADLTVFTGELDQFFDYKHGKLEYRSLDLVYQTFDQERYQPAAVVNYPN
                     DYDWTRITEYKYFLEEKSPKTTVCFEYPKASGEPYYIVMTKDNMEKRALYMQEVETLE
                     SQGHWRFIGRLAEYKYYNMDQVIAAALQKIAGV"
     gene            11374..12597
                     /locus_tag="PLQ30_03600"
     CDS             11374..12597
                     /locus_tag="PLQ30_03600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW01559.1"
                     /translation="MNKLKDKLITGIFNDSFAPITDGVAMVTKQYALWLNRKAGPSCV
                     VTVDSSDYYDNEEFPVYRIPSVALAARPPYRAGITILGELAEKRLRGGILKEKLTINV
                     FDIPFDLVHSHCPFASGMLAQNIARRRDIPLVTTFHTKYRPEFEQALKVKPLVAIAMQ
                     YVREYYKRADFVWVPNKETIPILRDYGYSGDLEVMPNGTDLFADVSEIPLLRQEGGVL
                     LNVPDDRFVFIYVGQHIPEKNVDLIIEALNVLDQRGIPFKMVFIGDGKYRGAMEKRVG
                     ELGLSQKVEFRGIVRDREVIKRAYARADLMLFPSLYDTSSLTIKEAAALNLPAVVIRN
                     SPTAEGIVDGQNGFLGDNDQRDYAETIARAIQNESLRKAAGEQARSTLYSSWESVIDI
                     VQNRYAEIVRSWHRR"
     gene            complement(12793..13482)
                     /locus_tag="PLQ30_03605"
     CDS             complement(12793..13482)
                     /locus_tag="PLQ30_03605"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HPW01560.1"
                     /translation="MKTIGLIGGMSWESTVTYYSIINSRINAELGKNHSAQCIVYSFD
                     FQNIEELQYAGKWAELEVELIKAAKALKAAGADFLVICTNTVHKVADNIEKEVGLPLL
                     HIADVVGEAVQTDGRQRIGLLGTIFTMEEDFYVRRLESKFGLHVLVPEKNDRQVINNI
                     IFSELVKGIVKERSKNEYLRIMDILKNQEAEGIVLGCTEIGLLIKEYDLPLYDSAVLH
                     ANKAATMSLIP"
     gene            14138..15526
                     /locus_tag="PLQ30_03610"
     CDS             14138..15526
                     /locus_tag="PLQ30_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPW01561.1"
                     /translation="METKTEVARTPSFLNAQTPAEMRRVILKLAMPAMIEFGLMMLVQ
                     IIDMIQVGVLGPWALAATGLVAQPTMLIFSVFSALVVGTTALVARAAGAGDSEYASRV
                     TRQSFVIVITMSLITTAVCIPASGSIVRLMGADIDTIEPATIYMKIVMAGTIFTVGNM
                     VLAAALRGAGDMVTPMISNLIANAFNLFGNWVLINGKLGFPRLEVAGAAIATTFSRVI
                     AFSITLHAVYRRDRFIHISIHDSYKVEPEILKKVLTIGLPAAGEQLIMRGGALLFARL
                     IAGFGTIMFAAHQIVINIDGLCLVPVMAFQMATTTLVGQGLGAKNPEFSEKATRQSTI
                     VSVSIMMLIGVLLFFFGNYAVFVFTRDKQVIEFGANAIKILAISQPIAAVFSVLGGAL
                     RGAGDTKYTMYISAIGVWVVRLGLGYLLADALGLALIGAWIAVVADMLVRALLAQRRF
                     SSGNWHKDIKVG"
     gene            16011..>16449
                     /locus_tag="PLQ30_03615"
     CDS             16011..>16449
                     /locus_tag="PLQ30_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01562.1"
                     /translation="MRYLYSTMLDNEQEKDNEGGTQDSKSNLSHLFRIALGFAVVSAL
                     ILIYLLVPRSGPAPRILAGGLASGPSMIVQELSVLRSAYPGIKIENTFDPIKSDWKII
                     ISKDDKTMELYWAEGRYLPANLLGSKEKYRKLIFYIPKKLADPA"
     assembly_gap    16450..16549
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16972..18207
                     /locus_tag="PLQ30_03620"
     CDS             16972..18207
                     /locus_tag="PLQ30_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW01563.1"
                     /translation="MQRKLLFAVLATMLIFSSAAQEKIIKIGTLFPMTGSVATAGQRC
                     TAAVETVVDIINGNYPNLDLPIAKKAGLLDGYKIVLVKADHQGKPDVAKSEAERLYEQ
                     EKVFAIIGCYNSSASKPASAVAERLQKIFMCGSSSSAELTERGYNYFFRLAPTDRIES
                     YEFVEWFQELNKSRNANIKTASVIYENTEFGKHAADEAKAACAKAGIKIIGDVPFSPG
                     ATNLNSEIQKLKAANADALFGAVLGADYSLMVRTMKQINWKPKIAINYCSGYQDPNIM
                     QQLGKDGWYFMGSAGYTPEFGLRFMKAVAPVEEIYKKKSNVPFDSNSIQEAVAMYVLA
                     LAIEKAGAVDTEKVVQVLRTTTFDSPLSMGGKVQFESPGGQNIKAQSVISQIQNGSYV
                     TVYPYEYKDGDPIYPMPAW"
     gene            18435..19292
                     /locus_tag="PLQ30_03625"
     CDS             18435..19292
                     /locus_tag="PLQ30_03625"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPW01564.1"
                     /translation="MSLFFQILLDGLWSGLLYALISSGLSLIWGVLDVINFAHGEFLM
                     VGMYVSFFLGFLFKMDPLISWVASGAFLFILGVLTYKLVIKRTLKMAMAPLLASFGLS
                     MVLKNIAMNRFTPNFRILSGTIMEGKVISLGSVIIPVPNLVTAIFSLVILGILYWMLT
                     KTRFGWAVQATAMDPEAAQLMGINTEHIFVLVFGLGGACVGIAGGLLPSYLATHPDVG
                     TLFGLIAFIVVALGGFGSIPGALVAAILVGLIESFAGFYIAPVFKYIAVFGVYLVVIL
                     IRPKGIKGW"
     gene            19296..20360
                     /locus_tag="PLQ30_03630"
     CDS             19296..20360
                     /locus_tag="PLQ30_03630"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPW01565.1"
                     /translation="MQKSEYQDCRQKKPRFIPWLALVLALALLPIIGIIKGNFLSHVL
                     VMILLNASMAQAWNIIGGYAGQISFGHSVFFGIGAYGAAYAVTTLKITPYPGMIVGAL
                     IACVVALIIGYPTFRLKGHYFAIATFAIVEVFNRLFMIWDTVGGAIGLDYPILKDGWR
                     NFTWSRTKVGYYYGALILFVLIFSIVRTMEHAKFGYYLKAIREGQETAESLGVNSRRY
                     KLLAMALSAAMAAFCGAFFAQYNYRVDPSMVLSLDMSMKFVLITILGGIGTLWGPLLG
                     AAILIPLQEYTRAYLARLGPGIDLIIFGIIIILMMEFQPRGLMGILENLRKPSRALVK
                     RSNTNSVQNNRPKGHLGGKQ"
     gene            20357..21145
                     /locus_tag="PLQ30_03635"
     CDS             20357..21145
                     /locus_tag="PLQ30_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW01566.1"
                     /translation="MSALLEIRNVTMKFGELVADRDVSFNVEAGTICGLIGPNGAGKT
                     TLFNCIAGYYKPSKGEIFFKGNRISGLPPHAITKLGIARTFQVVKPLKEMSVLDNIMV
                     GAFLHHSRASEARKIAQYCLSLCFLEDFSDKPAGELPIGSKKRLEIARALATEPSLLL
                     LDESISGLTSTEIHEMLEMIRRLRDKGITILMVEHIMEAIMPLANTIVVLSHGEKIAE
                     GQPQSIVHNQEVITAYLGEKFVKKMNMQNQIMLTDAGDKKGDAP"
     gene            21142..21858
                     /locus_tag="PLQ30_03640"
     CDS             21142..21858
                     /locus_tag="PLQ30_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW01567.1"
                     /translation="MSEIILEVKDIHCEYDNVPVLHGVSLRVCKGEIVAMVGSNGAGK
                     TTTLRTIAGLLHPVSGSIRFMGKDITRTEAYALVRNGISYIPEGRRLFGKLTVRQNLE
                     LGAYTTKERSIIQKRLHEVFQLFPILDERQNQTAETLSGGEQQMCAIARGLMSDPALL
                     LLDEISLGLAPSLVEAVFDTIIKIRDQGITILLVEQLVQESLEIADRGYVIQTGRIVH
                     DGPSHELLEMPEIRAAYMGM"
     gene            complement(21960..>22169)
                     /locus_tag="PLQ30_03645"
     CDS             complement(21960..>22169)
                     /locus_tag="PLQ30_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01568.1"
                     /translation="CWNPSPLLEYIGQYIQSIQIRLTYKAEKEHYLTKSEIVIFEFLI
                     VFQHWEIGPKKSHELIRLNPYSRTN"
BASE COUNT         5732 a         5003 c         5280 g         5954 t
ORIGIN      
        1 tcatgaaaca tcaacaacag aatcctgcca cgcaattaag tggacatttc tctttacagg
       61 agacctactg atcatctcaa ttctatttat tctcataaca acgttttgaa gcttttctac
      121 gaccagtcta gattacaacg ttgcgaacgc gattctgctc tattcaatct cttttgcttt
      181 atagcatata ttgacaagag taagattgta ttctataggc tctttcatat agcgagcaaa
      241 attataaaag accgaggaaa gaaaattgca aaatccattc tatgtgcctt tgtctagaaa
      301 gagtgctttc ctttttggca ttgtagtatt ctgccttatt gcaccttttg ccttcgctca
      361 ggaaaatcag acaagtcaac cggataagaa cgatttattg agagtctctc tcagcgcgtc
      421 tttggtggtc actaacaccg acgaaaaagg agtcgtgcgc gaaaccctgc aaccattgcc
      481 cgacaaggtc tatcctggcg atgtcattca atactcgata accgcaatca atacttctac
      541 cggctgccct tgtactgaca tgctcaaaaa tatcgcgctt atcggcacaa ttaacaaagg
      601 caccgtctat attgaagaat cagtgactct tccccctttg gcgatgttct cgatcgatgg
      661 gggtgtgagt tatcatcctt ggcctatcag ctatatcgtc gagactgcag atggctctga
      721 gacagtcaag acggctaccg cggagcaata taccaacatc caatggatta tccctccgct
      781 tgggccacag gagcgtctta cagtctccta cagagttagg gttgagtgag agacaagagt
      841 ataactgtgt tttgaattgg gaactttgaa ggcaatgatc atctcttttg cgccagagaa
      901 taagctatga aaagaaaagc aagctttcta atttgtttgt ctttacttct tgctgtaatg
      961 acatcctggt ctgagaatgg agaagcacaa tcaatctatc ctgagagcgt ctatgaactt
     1021 ttagcgagca agcaagatat tattctcatc gatgtacgta ctcatgatga gtatgtagca
     1081 ggtcatattc caggggccat tctattccca gtagcagata tcaatagcga aagtgcggca
     1141 aagataatcg gtcctgacgt tacaagaatg gtgatcgcat attgcgcatc aggaaataga
     1201 agcagaacag ccacctcgct ccttcttgac cttggatata tgaatgtatg gaacatgggg
     1261 agcctttctg catggccttt tgagatagaa catggagagc ccctgctcgt cccttgactc
     1321 gactgaaacc tgccataata caagcgctct ttattggtcg ttaggaaaat gcctctctag
     1381 ctgacttttg aaagagcctg ataaaagcta tgattgctcc tttttgctct tgcgtaagca
     1441 cgatagggcg cgctatggtg aaagagtcat gataaatgtg cctgcgcttg ccaaaattct
     1501 cgtcattttt gcgcttattg tgattgcagc ctctaagcat gtgcacctag gccttgctgc
     1561 tgcagtcggt gggattctca tcgcgctgtg gcaaggaatg aatactgaag ggattgtcaa
     1621 tgccgcattg atggagctta ccaatcctga tttgatattg ctggtgctcc ttttggcagc
     1681 tattatgact ttttctgaag cgatgaagat ctcgggggcg atgaatgtct tttccaacac
     1741 agtcgctaag gcaatgcctt cgcgcaggat agcgctcggg gtggtgccat tgctcatagg
     1801 aaccctgcct atgccaggcg gggcaattat ttcggctccg ctggttggtg cattgaatga
     1861 tgatgatgcg cgagggccgg aggcgctttc tgcggtgaac tactggttta gacatatcct
     1921 cgaactcgcg tggccgcttt tccctgcgtt tattttaaca tcggggctga caaaacttcc
     1981 tacgctcacg ctgattggtc tcaatatcta tgcgccaatt gtgctcttga tattgggttt
     2041 tctgtttgtg ctaccaaagt cgagaaataa ttctgagggt gcccatggct cggcagatgc
     2101 gataccttct ctcccgaacg cgattccttt atctatggca acaccttcca gctgcgggag
     2161 ggcttcagcg gacagccttt ctgggaactc gatctctata ggggaaaaac catctattgc
     2221 agaggtgcta catggcatcg cgccacttgg gctcgtgctc ggtacctata tagtaatcga
     2281 tgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngggattc cggctcttgt
     2401 cgtcatcgca gttctgccat ttatttcggg tatcgtaacg ggcgtgggga tggggtatgt
     2461 cgggttatca atgcctatcg tactcgcgct cattgcatcg tcgggaattc cattaaaggc
     2521 tggggtgatg attgcgaatg cctttggtta cacaggaatg atgctttcgc cacttcatgt
     2581 ctgcatggtg gtgaccgctc agcactttaa gtcgtcactg ataaaggtga ttctgaaatg
     2641 tgctttgccg ctcgcaattt ttctcattat tgtaattgca tatggcgccc tattgatggc
     2701 actgatgaga tgaggttttg aagaacacta atcgccgaac gaagtattca gcgatgcctt
     2761 ttccacattt cgctcttgta actaaagctc gaacaggaat ggatcgatat atatcgagaa
     2821 atcgaatgat actttgtctt tgggctccat cctgaagtgc ttggccctag caagaacact
     2881 atagcacgct atctcgattt actggaaaaa ggctttcatc gtcagaaaat ctgtgcattt
     2941 tgcgcaatct cagaggtgaa attagcaaga gtgcgaaata ctatttttga gatgtcggag
     3001 tgagaaatgt ggtcaccaat ttggagtgaa aattttcatc gaagcaccta ttctttcctc
     3061 cagcgcatat aagcccttga caaaatccaa gactaaatga gatattatat cgatatcaat
     3121 atgatatcat taattcgaaa aagggaggcc attatgaacc aaagtcccct aaatgaagaa
     3181 aagtcagcac caagcttaag tggcatggcg atgcttattc tcgatgtctt tcttatgatc
     3241 gtgaccgttg ctggttttgt ttttggaaca tcttcgggaa aaacggatgc gggtacgatc
     3301 gtgctcatgg ttgtctgtgg cctatacgca ttcttgatcg ggcctattct ctttgctggc
     3361 ctcaaggtcc ttaagcctaa cgaagcaatg gttctgacgc tctttggcaa ctatcatgga
     3421 acgctaagag ggcctggctt cttctttgta aatccctttg ttgcatcggt ttgtccttct
     3481 aatgtacagc tttcatcggg atccatcccc cttccaatag gtgagacggg ttctagcagt
     3541 gctgctcaca aaacccatgg tgcctcttcc aaacaagctt ccaaaaaaat atcactcaag
     3601 gccatgactc tcaacaatga gaagcagaag gtcaacgatg ccttaggcaa tcctgtgatc
     3661 atcagtatcg tagtgatatg gagagtcgtc aacaccgcaa aagccgtatt caatgtggac
     3721 aattatttcg aatttctctc tattcagtgc gactccgctg cgcgcaatat cgtcaggctc
     3781 tatccctacg atgcttccga agaagaaggg gaaaagtccc ttcgcggaag ctgccaggag
     3841 atagcagaga gccttaaaga agaggtccag aagaaagtgg acatggcagg gttggaaata
     3901 ttggaagcgc ggatcactca tctttcttat gctcccgaga ttgctgccgc tatgcttcag
     3961 cgtcaacaag cttcagctat cattgcggct cgccagatga tcgtagaggg tgccgtaggc
     4021 atggtcgaga tggctcttgc taaattgagc gacaggaata tcgtaaactt ggacgaggaa
     4081 cgaaaagcta ctatggtcag caatttgctt gtcgtattgt gcgctaataa ggatccgcag
     4141 ccaatagtca atactggttc tatatattag ggttaatgcg ttagagttag gagattagaa
     4201 ccaaggggtt aaggtcaaga ggcatgattt aagaggcgcg gctttgagag gactttggga
     4261 atctaagtcg agggaaaagt atggaaggat tggaaaaaga taacgagaag aaacagatcc
     4321 tccttagact ctctccgacc ctttggaaag aactggctgc atgggctgca gatgattttc
     4381 gctccataaa tggccagatt gagtatctct taacggaatg tgtgcgtcaa cgtaagaaga
     4441 agaaaattga ataggatact gtaaggaggt cagctacgat aaccgtcgag aaaatatgca
     4501 agacctacga aagcattaag gctgtggaag gtttatcctt cgaagctgtg cccggtgaga
     4561 tctttggtct tattggccct aacggagcgg gcaaaacgac aacgatccgt atgataatga
     4621 acataatcgt gcccgattcg gggatcattc gcttcaatgg agcacctttg tccgaagcag
     4681 acaaagatca cataggctat ttacccgaag agcgaggcct gtatcgcaaa gccaagttgg
     4741 gcgaagttct ggaatacctc gctgccatca aaggagcaaa ggcgaatgaa taccgtcctt
     4801 ccataaatgc atggctcgag cgattcgggc tctccgaatg gaagaatcgc aagatttcgg
     4861 atctatccaa gggcatggcg caaaaagcgc aattcattgc gacagtagct cataatcctt
     4921 cggttgtatt attcgatgag cccttctccg gccttgaccc tatggcacaa gacgatctcc
     4981 tttccgtaat ggtcgagcta aagcataagg gcgcgatagt actcttttct actcacatca
     5041 tggagcacgc tgaaaagatc tgcgaaaaaa tccttctcat gaataaaggc aaggccatag
     5101 tatatggatc gatggctgat atcaaggcgc aatatgggcg caatgcggtt cagctggaat
     5161 ttgacggaga tgcttctttt gtgcagagcc ttgattttgt agactcagtg gtgtcctatc
     5221 cgcgctggat cgaagtcgaa ctaaaggaag gcaccgactc cgacctttta tttaaggctt
     5281 tggcaggcag gctcaaggtt cggcgctttg aaacagtagc cccatccttg cacaagatat
     5341 tcatccatct ggtaggagga gaggagattg agaatgggta agctaaaaca tattattcgg
     5401 catgaattca ggctcaccac tgcaaacaaa gcctttgtca tcctaaccat cattgggcct
     5461 tttctcatct ttgcaataac tgtcctgccc actttgctcg ctcaatcttc gagcccagta
     5521 gcatcggata agccgcttgc cctcttcggt gctccaccta gggtcgaaca agctatttca
     5581 gctgcatttt ccggcatgaa actccaggtc gagcacgtcg aagacataga agcagctaaa
     5641 gcttcggtaa tcgatggttc tcgagttgcc ttgcttgaac tggaggcggg atggcctgac
     5701 acaggaaaag ctatctggta ctcaaagagc agcgcagaaa taggattata tagcacggct
     5761 tcagcggtct tagaggctgt agcgcgcgaa gagcgaataa gggaaagcgg aatagaccca
     5821 gctctggcag caaagctgct ctctaagccc actttcgagg taataaagct cgaaaaggga
     5881 ggcgaggaga aggctcaggg ccagggagat caggcctttc tcgagacgct tatgacagtt
     5941 ctagtattta taatgttgct ctatatgact atattgctct atggccagat gatagggcgc
     6001 tctgttgtaa ccgaaaaaac ctcgaagacc gtggagatta tgctctcgag cgtgacgagt
     6061 cgagatctca tgataggcaa aatattgggt ttggggctcg ccggtatgct tcaatatgcg
     6121 gtttggatat cgatggcgtt tgtcctcaac cactttctgg ggccgctctt cggactcgct
     6181 ttgcccgtgg cagtatctat gcaaaatatg atgtggctgt tcgttttctt tatcctgggc
     6241 ttttttctct attcctcagc atacgcagct ttgggagcag ccagcgagga tgagcagcat
     6301 ctggggcaga tagcatggcc attattgatg ttcttgataa tacccatggt tatgctcaat
     6361 acgctcgtga cgagccccga ttcaacggct gtcgtaatca tgtcgttttt tccacttacg
     6421 agtcctatcg tgatgctatc cagaatcctt gttgcatcgc ctccagtatg gcatctagta
     6481 ctttgtattg gcatccttgt tgcgtcgatt gtgggcatga tggtatttgc aggaaaggtt
     6541 ttccgagttg gcatactcat gacagggaaa aggcctaagc tcaaagaggt gttgcgctgg
     6601 gtaaaggtga aatagggttt ggtttacaca tgaggcgatt gtgttacaaa gttatctggc
     6661 aagttttgaa tattgtgtga tatattccgg ccgtcgaaaa tatcatccgg cagctgtatt
     6721 gtttttctct taccgttccg ccccgacttg tacatattga acaggttcaa gcggagcgcg
     6781 ttcgtcacga cgcagaagct cgacagactc atcgcggcgg ctccgagcat cggattaagc
     6841 acgatcccga atgcgggtat aagcgctccc gctgcaaccg gtatgccgat gatattatag
     6901 atgaacgccc agaacaggtt ttcccgaatg ttcgtaagta cctgacgcga cagtttgatt
     6961 gcggccggaa catcgacaag cgtggatttc atgagcacga catccgcagc gtcgagtgcg
     7021 acgtcagttc ccgcgccgat cgcaactccg gtgtcggcgc gcgtaagggc aggtgcgtcg
     7081 tttatgccgt cgcccaccat catcactttc ccgaacgccg aaagcttcgc gacgacggcg
     7141 tccttgccgc ccgggagcag atccgatacg acatgtgtaa tgcctacctg cttcgcgatg
     7201 gcttccgccg tacgtctgtt gtcgcctgtg agcatgacta cctgtacccc gagggcgttg
     7261 agtttctgca ccgcgtcggc gctgtccttt tttgccacat ccgcaaccgc aataattccg
     7321 agcattttcc cgccagctgc aaaataaagc ggcgtctttc cttcgccggc aagctccata
     7381 ccgcgctgtt tcatctcgtt gccgagcagc cgctggtctt tcataagcgc cgcatttccg
     7441 cctgatgccg gaacgccggc gacaggagcc acagctccgg aatcatttcc tgacggtggg
     7501 accactccct ttacgccgta cccaggcagc gcggtaaaat cctccgcagg gctgtatgca
     7561 atattttccg cccgtacttt ttccagcact gcgcgtgcaa ggggatgctc gcttttttcc
     7621 tcaagcgcac ctgcaagcgc aagcagttcc cgttcagtta ctccgtccgc gggaattacg
     7681 tccgtcactg tgggctttcc ttccgtgacc gtacccgtct tatcaagcac gactatatca
     7741 gtctttccgg cagcttcaag cgcggcggcc gtcttaaaca gaatgccgtg ttttgcgccg
     7801 agcccgcttc cgaccatgat tgccaccggc gtcgcaagac cgagcgcaca cggacagctt
     7861 attacgagca cgctgactgc acgcgcaagc acgaagccga attctcctcc ggcaacagcc
     7921 catattattc cggtaatcac cgaaagtaca ataacgatgg ggacgaatat gcccgacact
     7981 ttgtctgcaa gtcttgcgac aggcgctttt gtcgcggcgg cgttttcaac catggtgatt
     8041 atctggctga gtgtcgtgtc gctgccaact ttcgtcgccg tacacgtaag aaaaccgttc
     8101 tggttgagcg tcgccgcgct cacgtcgctt ccggccgact tgtcgacggg aagactttcg
     8161 ccggtaagcg ctgactcgtt caccgcgctc gttccgctaa tcacttttcc gtctacgggg
     8221 atacgctcgc cggggcgcac gataaacgta tcgccggtcc gcacctgttc cgcaggaatc
     8281 gtaagttcct ttccgtcgcg gatgacgtgc gccgtgtcgg gagcaagatc aatgagactt
     8341 ttgattgcgt tcgtcgtctt tcctttgctg tacgcttcga gtgttttccc gacagttata
     8401 agcgtcagaa tcatagctgc tgattcgaaa tacagatcgt gcagatactc atgcgcatca
     8461 tgcaacatgc cggaaagcat ggcaccggtt ataagaaaaa gaacgacaac actgtatacg
     8521 accgacgctc cgctgcccag cgcgacgagc gtgtccatat tcggtgcgcc atgaacaaag
     8581 cttttgaaac cgctagtaaa gaaacgctgg tttatcatca tgattgctaa tgtcaaaagc
     8641 agttcgtata accctattgc ggctggattt tcgctgaata cagcgggaag cggccacttc
     8701 cacatgaggt gtcccatcga aacatacaag agcggcacaa gcagaatcag cgaagcgata
     8761 agccgtttca cgagacgcgg cgtctcgtga tcatcgagcg catcccgttc agaagcccgc
     8821 ttccttgccg cgtccgcagc ggacaaacct gagtccacat cttcgggcgc cgcaccgtat
     8881 cctaccttgt ccacagcagc acatatcgat tccgccgaag cgggagatgc aaactcaacg
     8941 ctcatcgaat tcgtaagaag actgacggac acgctcttta ctccgggaat cgcggcaacc
     9001 gccttttcga catgagcgct gcacgcggcg catgtcattc cggtaacaga gaatttcgtc
     9061 atatgtatat ctaccatcct tgaaagttat tacttcctat ttcaattttc taatcagttc
     9121 gagggcttcg tcgagaatct gcatgttatc tttctttatt tcttccacga cgcatgtttc
     9181 gagatggttc cgaagaatga tatacccaag actctgaagc gcactttcaa cggccgcaac
     9241 ctgcgtgagc acatcgccgc agtaccggtt gtcgtcgagc atcgttttta tgccgttgag
     9301 ctgccctatt atacggttaa gcctgttctg aagttgcttc aacgtctgtt cttcgcgcgg
     9361 agtgtttttg tagcggcagc tcacacaacc gccttcatcc gctacctgcg ggacgtttcc
     9421 accagcatct tcattcattt ggctgcctcc tgtcttttat atacccccat ggggtatctt
     9481 ttcgtttaga ataaatataa taccccatag gggtatctat caagtacaaa agaaaagacg
     9541 tcccaaaagg cgtattcccc accagcatta cggctccacc cacgccggca attctttgcc
     9601 gaatcctctg cattcccggt cgccgaacaa ctcttaatag gcgcatctgt acaaaatgaa
     9661 gtttgtgttt accaaaccag agattccaag gtcctacttc aaatacaaca agcgcctgtt
     9721 cggagaagtg tccaagctgc cattttcctt gctctcgtag tttttatccc tggcagcaag
     9781 gccatctgac tcagtcccaa atagctacgg aaatgccaat gatggatgcg gaatctgcag
     9841 aagtcccaga tacttgggcg aagcttcgca ggcaaagtcg ggcacgactt ttgcagaagg
     9901 tctacgaagt ggatccaatt gtctggccaa aatgcaaggg gacaaggaga caagaggaca
     9961 aagtcggtgg tggcgctagc cgtattcatg cgattttttg aattgtacta aaacaagggt
    10021 tgacgataga agcagactag atttattttt tgtgctggcg ttaccgaaaa atttggaagg
    10081 agcagtcctt tgggtagcgg gatcggttgc agttcggggc aaacgcgaat tccatccaaa
    10141 aaccgcaaaa ttccagaaat gacagttcct atgaataaag attttttgtt accgctctta
    10201 aacatttttt atgaaagatc ttatgagctg gatctgcacg gcttttgtga ttagtattat
    10261 ctaaggagga tcgatgaaat acttcgaagc tcttgttgtt ggtgccgggc ttgccggcag
    10321 caccgcggca cgtattttgg ccgaacaagg ccgcaaggtt ctggtggtcg aaaaacatcg
    10381 acatatcgcc ggacattgcc acgacgccag gaatgaagcg gggattacca ttcacaccta
    10441 tgggccccat attttccata ccaataacaa gaccgtgtgg gattttgtgc atcgttttac
    10501 cgaattccgc tattttcaac atcgtgtgct ttcttacgta gacggtcggt ttattccatt
    10561 tccaattcag gcggacacaa tcaacgagct ttttggaacg agcattgcaa cccaagaggt
    10621 ttcggatttc cttgcggaag aggttcggca atcgaaattt cagactcttc ccgctacctt
    10681 tcgagatgtg gtagtgtccc aggtcggaga acgattttat tcgctttttt ttgaaaatta
    10741 cacacgcaaa caatggggcc tggatccgac actgctttcc gcagaagttg cttcgcgaat
    10801 tccggtacgg acaaatcgag atggtcgcta tttttcggac cgatatcagg gcataccggc
    10861 gcacggctac acggcaatgg tggacgaaat gctcaatcac cccaatatca gcgttctctt
    10921 ggggcttgat tggttcaata tcaaggacga aatagcggct gaccttactg tttttacggg
    10981 agaactcgat caattctttg actataagca tggaaagctt gaatatcgat cgctcgatct
    11041 cgtttaccag actttcgatc aagagcgcta ccaacccgct gccgtggtca attatcccaa
    11101 cgactacgat tggacacgca ttacagagta taagtacttt ctcgaagaaa aatcacctaa
    11161 gaccacggta tgcttcgaat acccaaaggc ttctggcgag ccatattata tcgttatgac
    11221 aaaagataat atggaaaaac gcgctctgta catgcaggag gtcgagactc tggaatccca
    11281 agggcactgg cgcttcattg gccggcttgc agaatacaag tattacaata tggaccaagt
    11341 aattgcggct gctttgcaga aaatagctgg agtgtgaaca agttgaaaga caaattgata
    11401 accggtatct ttaacgactc ttttgctcca atcaccgatg gcgtagccat ggttaccaag
    11461 caatatgcgt tgtggctcaa tagaaaggcg ggtccttctt gcgtcgtcac agtcgattca
    11521 tccgattatt atgacaacga ggaattccct gtctatagaa taccttcggt agccttggct
    11581 gctcggccgc cctaccgagc ggggatcacc attctgggcg agcttgcgga gaagcgctta
    11641 cgcggcggaa tcttgaaaga aaaactcact ataaacgtct tcgacatacc cttcgatctt
    11701 gtgcattcgc attgtccgtt cgcttctgga atgctagcac agaatattgc gaggcgtcgg
    11761 gatattccac ttgttactac ctttcacacc aagtatcgac cagaatttga acaagcgctc
    11821 aaggtcaaac ccctagttgc aatcgccatg cagtatgtgc gcgagtatta caaaagggcg
    11881 gatttcgtct gggtaccaaa taaagaaaca attccgattc ttcgcgatta tggatattcg
    11941 ggcgatctcg aggtaatgcc gaatggcacc gacctttttg ccgatgtctc cgaaattcct
    12001 ctcttgcgac aagaaggtgg ggtacttctg aatgttccgg acgacaggtt tgtttttatc
    12061 tatgttggac agcacatacc agagaagaat gtagacctga ttattgaggc gctgaatgtt
    12121 cttgaccaaa gaggtattcc ttttaagatg gtctttatcg gtgatggcaa atatcgtgga
    12181 gcaatggaga agagggtcgg tgagttgggt ctttcccaga aagtggaatt ccgcggcatt
    12241 gtgcgcgata gagaggtcat aaaaagagcg tacgcccgag cagatctaat gctcttccct
    12301 tctctttatg acacatcgag cttgacaata aaggaagcgg ccgcgctcaa tctgccggcc
    12361 gtcgtaataa ggaattcgcc caccgccgag ggtatcgttg atggtcagaa tggcttcttg
    12421 ggcgataacg accagcgcga ttatgccgaa actattgcac gggcgattca aaacgagtcg
    12481 ctgcgcaagg cagcaggcga gcaagcgaga agtaccttgt actcttcctg ggagtccgtg
    12541 atcgatatcg tacaaaaccg atatgccgaa attgtccgct catggcatag gcgctaggcg
    12601 tgccatggca agcaaaccct cgcgccgcac agcagtaagg cctttttggt caagataaca
    12661 agataacatg gatgtaaggt ccaaagtctc attagtgtac ctacttcaat ttcgattttt
    12721 cccaggggaa tgggctaggc tttggttcat ggaccagccc aaccccagaa cattgtttcg
    12781 agcttcaaga attcaaggta tcaaactcat agttgctgcc ttattggcgt gcagaacggc
    12841 ggaatcgtaa agaggcagat cgtattcttt tatcaaaagc ccaatttctg tacagcccag
    12901 gacaatgcct tcggcctctt ggtttttcag tatgtccatt atgcgcaaat attcattctt
    12961 ggacctttcc ttgacgatgc ctttaacgag ctcggaaaag attatgttgt tgatgacctg
    13021 tcggtcgttc ttctctggca cgaggacatg cagcccaaat ttcgattcga gtcttcttac
    13081 gtagaaatct tcttccatgg tgaatatcgt accaagcaaa ccgattctct gtcggccatc
    13141 ggtttggaca gcctcgccaa cgacatcggc aatgtgcaaa agtggcaggc cgacttcctt
    13201 ttcgatgttg tcggccactt tgtggacggt attggtacaa atcaccaaaa aatcggctcc
    13261 ggctgctttg agcgcttttg ccgcctttat gagttccact tcaagttccg cccacttgcc
    13321 cgcgtattga agctcttcaa tgttctggaa gtcgaaggaa tagacaatgc actgggcaga
    13381 atggttcttc ccgagctcgg cgttaatcct cgaattgata attgaataat aggtaaccgt
    13441 cgattcccag ctcatgccgc caatcaaacc aatggttttc atatttatca ccttctagtc
    13501 agactcccct ataatagaac caaaggggct agagtcaata gttttaaggc caaggatgtt
    13561 gcggtacttc gcaggttgag ggcgatctcc acttgtgagt gcgcacaatt ctgtttagac
    13621 agcctgatgt tgatgagctt ttttctatat ataattatta taattaagga aaacgaatgt
    13681 ggacgacaag aatgggaaaa cagtgaaaag cgctatcgtt gtgggttgag gaatgggcgg
    13741 gccagccgct gccgcagatc ttgcaaaggc cggttttgcg tacgaagtct tcgaaaagta
    13801 aagccaggca tggggcgaga tgggcttaac agaaaaggat tcattccgat tcgatcttga
    13861 gccgaccata gtcatgatgc cagacatcaa tcgcgaggta ttcaatgagc cactaaatca
    13921 cgaaaaatag cgggtctcgt tctgcaatga aatccaatag cctattttca aagaatgtgc
    13981 tatctttggc cttggtcgtt ggttgtcctt tttttgttag tttagataac aacattctgg
    14041 agcttccaat gaccttcttc aactaccaaa ttgcaaactt aattctgcag tatacgaaga
    14101 gataaacgaa tttaatgagt ataagtatgg agatttaatg gaaacaaaaa ctgaagtagc
    14161 tagaacgccg tcatttttga acgctcaaac tcctgctgag atgcgaagag taatactgaa
    14221 attggctatg cctgcaatga ttgaatttgg ccttatgatg ctggtccaga ttattgatat
    14281 gattcaggtt ggagtcttag ggccctgggc tcttgctgct actggtttag ttgcccaacc
    14341 gaccatgcta atattttccg tgtttagtgc gttagtagtt ggtactaccg cacttgttgc
    14401 gagagcggct ggtgctggcg acagtgaata tgcttcacgt gtgacgaggc aatcttttgt
    14461 gatagtgatc actatgtcac ttattacaac cgcagtttgc attcccgcct caggaagcat
    14521 agtaagatta atgggagcag atatagacac aattgagcct gcaacaattt atatgaaaat
    14581 agtgatggca ggcacaatat ttactgttgg caatatggtg ttggcagctg cattgagagg
    14641 tgcaggcgat atggtaacac cgatgatttc taatttgatt gcaaacgcat tcaatctatt
    14701 tggaaattgg gtacttatta atggaaagct tgggttccca agattggaag tggccggcgc
    14761 ggcaatagct actacatttt caagagttat agcgttttct attacgcttc atgcagttta
    14821 tcgcagagat agatttattc atatttccat tcatgattcg tataaagttg aacctgaaat
    14881 acttaaaaaa gttttgacta tcggactccc tgcggcagga gagcagctta ttatgagggg
    14941 tggagcattg ctttttgcaa gattaattgc tggatttggc actataatgt ttgctgctca
    15001 tcagatagta ataaatatag acgggttgtg tctcgtgcct gtaatggctt tccaaatggc
    15061 aacaacaaca cttgttggtc aaggtttggg tgcaaaaaat ccagaatttt ctgaaaaagc
    15121 aacacgtcaa tcaacaatcg ttagtgttag tattatgatg cttattggag tgctgttatt
    15181 cttttttggc aattatgctg tatttgtttt tactagagat aaacaggtga tcgaatttgg
    15241 agcaaacgca ataaaaatac tagcaatatc tcaaccaata gctgcagttt tttcggtact
    15301 tggaggagcc ttacgcggtg ccggagatac caagtatacc atgtatataa gtgcaattgg
    15361 agtgtgggtt gtacggttag gacttggtta tttgttggcg gatgctcttg gtcttgcact
    15421 cataggcgca tggattgctg ttgttgccga tatgctcgtc agagcactgc ttgcccaaag
    15481 aagattttca agtggaaatt ggcacaaaga tattaaggta gggtaaaatt agaggccgta
    15541 tttattggta ttgtcaacaa tcttatagca catttcccag gtcgccctca tctggcttta
    15601 tgctgagcat ctaggatatg gtagccgatt gtttctttga ataagagatc catgttcatg
    15661 tatcgcagcg tattcaattt tcgtcccgag atgtatttca ggcgtcaacg acaagcataa
    15721 gttttgagtt tctcggcgac cgcattggct ttctccaatt gggttcatgg cgtcacccct
    15781 tcttggtcaa gcttggtctg gtcactgaac ttaaccagaa tagggtgtcc tttttcaatt
    15841 tctccaaatg tgcaaaaaat agaatacgta tcaatcaaaa agctattgcg cagcctaaag
    15901 gcgaaatagg ggttgatcta tgcatgaagc gattgtgtta caaagttctg ccgctaatca
    15961 aaagattttg acatgaaaga aatctgctct cttgacatag cgcaaggcag atgcgctatc
    16021 tttatagcac aatgcttgat aatgagcagg aaaaagataa tgagggaggg acgcaggatt
    16081 caaaatccaa tctgagccat ctattcagaa tcgctctcgg atttgcagtt gtctccgcac
    16141 tcattctaat atacttgctt gtgcccagat caggtccagc acccaggatc ctggccggag
    16201 gcctggccag cggcccttcc atgatagtgc aagagcttag tgtccttcgc tctgcttatc
    16261 caggtatcaa gatcgaaaac accttcgatc caataaagag cgattggaaa attatcatca
    16321 gcaaagacga taagacgatg gagctttact gggcggaggg acggtatttg ccagcgaatc
    16381 ttcttggctc taaggaaaaa tatcgcaaac tcatcttcta tatccccaaa aaactcgctg
    16441 atccagccan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ttatctgggg
    16561 tggcaaatgg acagtctggg ataatatgca tttcgagtat cggcctgagt tgatcctggg
    16621 aagggagctt gtaaagccat aattgcagta ctttccctat gcatgaatca gattgatgca
    16681 tgcatcagat tggttcatag atttcatttt tcgccacgat ttgtgcggga aaagctgtcc
    16741 aggaagctct gcgaaaccgg ccagatccaa aaacgctcaa tcccctatct ctttatcaac
    16801 taggcaacta tttcaaaaag tatttgacag cggtgcaaga acgattctat actacgaaaa
    16861 tcgattgagc ctatttcaat atcaattgag cccaccataa gaaggttatt tgcaagcaga
    16921 ttgcttgcca gatatatgag ggatcagttc tatttaatag gaggtaagaa tatgcaaagg
    16981 aagctgttgt ttgcggttct tgcgacaatg cttattttca gctcagcagc acaggagaaa
    17041 atcatcaaaa ttggaaccct gttcccgatg acaggatcag tcgccacagc aggacaacgt
    17101 tgtacggcgg cggtagagac tgtcgtcgat atcatcaatg ggaattatcc gaatctcgac
    17161 ttgcctatcg ccaaaaaagc aggactgctg gacggatata agattgtgct cgtcaaagca
    17221 gaccatcaag gaaagcccga tgttgccaaa tcggaagcag aacgtctcta cgaacaggag
    17281 aaggtctttg cgataattgg ttgctacaat agctccgcct cgaagcccgc cagcgccgtg
    17341 gccgaacggc ttcaaaagat attcatgtgt ggttcctcaa gttccgctga acttacagag
    17401 cgtggctata attatttctt cagactggcg ccgacagacc gaatcgaaag ctatgagttc
    17461 gtagagtggt tccaggagct aaacaaatcg cgaaacgcaa acataaagac agcctcggtg
    17521 atctatgaga ataccgagtt cggcaagcat gctgccgatg aagcaaaggc tgcttgtgcc
    17581 aaggctggaa tcaagattat cggcgacgtt ccattttcac caggtgcaac gaacttgaat
    17641 tctgagattc aaaaattaaa ggccgcaaac gcagatgctc ttttcggtgc tgtccttggc
    17701 gccgattatt cacttatggt cagaaccatg aaacagatca actggaaacc taaaattgcc
    17761 atcaattatt gctccggcta ccaggatcca aatatcatgc agcagcttgg caaggatgga
    17821 tggtacttta tgggttctgc aggatacacg cccgagttcg gattgcgctt catgaaagcc
    17881 gtcgcgccag tcgaggagat ttataagaag aaatcaaatg tccccttcga ttccaattcg
    17941 atccaggaag cggtagcgat gtatgttctc gcgctggcaa tcgagaaagc aggcgctgtg
    18001 gataccgaaa aggtcgtaca agtactccgt acgacaacct tcgattcgcc tctctcgatg
    18061 ggtgggaaag tacaattcga atctcctggc ggccagaata tcaaagccca gagcgttatt
    18121 tcgcagatcc aaaatggcag ctatgttact gtctatccat atgaatataa agatggcgat
    18181 cccatttacc caatgccggc ttggtaatca ttctgaatta tcgtccaagc ttgcttctaa
    18241 tcgagaatga tatgatgatt gtgcagtatt ccgataatta tagaaggagt atagaataac
    18301 agggatacaa ccaaaagagc ggctttgcac ctgcagagcc gctcttttat catgcaaaag
    18361 gcttttccga aatatgccag agcattcaaa atgagtctcc tttttaaaat gcagcatgtt
    18421 cctgaggagg tagaatgagc cttttttttc agatattgct ggatgggtta tggagcggtc
    18481 ttctctatgc ccttatttca tcaggacttt ccttgatctg gggcgtactg gatgtcatca
    18541 attttgcaca tggtgagttt ctcatggttg gaatgtacgt ctcgtttttt ctggggtttc
    18601 tcttcaaaat ggatccccta atctcatggg tcgcatcagg ggcctttctt ttcattcttg
    18661 gagtgttgac ctacaagctt gtgataaaac gaacactaaa gatggcaatg gctccgctat
    18721 tggcgagctt tggcctgtca atggtactca aaaatattgc tatgaatcga tttacgccga
    18781 atttccgtat tctctccgga acaattatgg aaggaaaggt gatatctctc ggttccgtaa
    18841 tcattccagt ccccaatttg gttaccgcga ttttttcctt ggtgatacta ggaattcttt
    18901 actggatgct aactaagact agattcggct gggcggtcca ggctaccgct atggatccgg
    18961 aagcagcaca gcttatgggg atcaataccg agcacatatt cgtccttgta tttggccttg
    19021 gaggagcgtg tgttggaatt gcgggagggc tcctgccatc ctatcttgcc actcatccgg
    19081 atgttgggac gctctttgga cttatcgcat ttatcgttgt ggctctcggt ggctttggga
    19141 gtattcccgg tgctttggta gcagccattc ttgtggggct tatagaatct tttgccggtt
    19201 tctatatcgc acctgttttt aaatatattg cggtgttcgg tgtctatttg gtagtcattc
    19261 tgattcgacc aaagggcata aagggatggt gagctatgca aaagagcgaa tatcaagatt
    19321 gtcggcaaaa aaagcctagg ttcattccat ggctggctct tgttcttgcc cttgcccttc
    19381 tgcccataat tgggataata aagggaaatt tcttatctca tgtcctggta atgattttgc
    19441 taaatgcttc catggctcaa gcatggaaca taattggggg atatgccgga cagatatcct
    19501 ttgggcattc ggttttcttt ggaataggcg catacggcgc agcttatgct gtaacaactc
    19561 taaagatcac tccttatcct gggatgattg tgggcgcgct cattgcgtgt gttgtcgcgc
    19621 tcataatcgg gtatccaact ttcagattaa aagggcacta ttttgcgatt gccacatttg
    19681 cgatcgtgga agttttcaat cgtctattta tgatctggga tactgtaggg ggtgcaatcg
    19741 gcctggatta ccctattctc aaagatggat ggcgaaattt tacctggtca agaacaaaag
    19801 tggggtacta ttacggtgcc cttattcttt ttgtgcttat cttcagtatc gtgcgaacca
    19861 tggagcatgc aaaattcgga tattatctca aagcgattcg agaaggccag gagacagcag
    19921 aatccttagg agtcaattct cggcgctata aacttttagc catggctcta tctgcagcta
    19981 tggcagcttt ctgtggcgca ttctttgctc aatataatta cagagtagat ccttcaatgg
    20041 tgctctccct cgatatgtcc atgaagtttg tgctgataac gattcttgga ggcatcggta
    20101 ccttgtgggg accattactc ggcgctgcca tactgattcc tttgcaggaa tacacccgag
    20161 catatcttgc caggcttggg ccgggtatcg atcttatcat ttttggaatc atcataatac
    20221 tcatgatgga attccaacct cgagggctaa tgggaattct tgaaaatctc agaaagccaa
    20281 gccgtgcctt agtcaagcgg agcaatacca attctgtgca gaacaatcgt cctaaaggac
    20341 acctaggagg caaacaatga gtgcccttct cgagatccgc aacgtgacta tgaaatttgg
    20401 cgaactcgtg gctgatcgag acgtgagctt taatgtcgaa gctggcacta tctgcggact
    20461 tatagggcca aacggagcag gaaaaacaac actcttcaat tgcatcgcag gttactacaa
    20521 gccatccaaa ggcgaaatct tcttcaaagg aaaccgaatc tccggccttc ctccacatgc
    20581 aatcacaaaa ctaggtattg cgagaacctt tcaggtcgta aagcctctca aggaaatgag
    20641 cgtcctggat aacatcatgg taggtgcgtt tttgcatcat tcgagagctt ccgaagcacg
    20701 caaaatcgcc cagtattgcc tttctctctg ctttcttgaa gattttagcg ataaaccagc
    20761 cggagagctt cccataggca gcaagaagag actagaaatt gctcgtgccc ttgcaacaga
    20821 gccctctctt cttttgctcg atgaaagtat ttctgggctt acaagtaccg aaatccacga
    20881 gatgctcgaa atgataagaa gacttcgaga caagggcata acaatcctga tggtggagca
    20941 catcatggaa gcaattatgc ctcttgcgaa tactattgtt gtcctaagcc atggcgaaaa
    21001 aatagcggaa gggcaacctc aatcgatagt gcacaatcaa gaagtgatca ccgcctattt
    21061 gggcgaaaaa ttcgtcaaga aaatgaatat gcaaaatcaa atcatgctca cagacgccgg
    21121 ggataaaaaa ggagacgccc catgagcgaa atcatcctag aagttaaaga tatacattgc
    21181 gagtatgaca atgtaccggt gctacatggg gtttcattaa gagtatgcaa aggagaaatc
    21241 gttgcaatgg taggctcgaa tggtgcagga aaaaccacaa ctctcaggac aatcgctggt
    21301 ctcttgcatc cagtttctgg cagcattcgc ttcatgggaa aagacataac cagaaccgaa
    21361 gcctatgccc tggtaagaaa tggcatctcc tatattccag aaggccgtag gctctttggt
    21421 aagctcactg taagacagaa cctggagcta ggcgcatata ctacaaaaga gcgctcgata
    21481 attcaaaaac gcctccatga agtatttcag cttttcccta ttcttgatga aagacagaat
    21541 cagactgcag agaccctttc gggcggagaa cagcagatgt gtgcaatcgc tcgtggctta
    21601 atgtccgatc cagctctcct cctacttgat gaaatatcac ttggccttgc gccctccttg
    21661 gtagaagctg tattcgatac catcattaag atacgagacc agggcatcac tatcctccta
    21721 gtcgaacaac ttgttcaaga atctttggag atagccgata gaggctatgt catccagaca
    21781 ggccggattg tgcacgatgg accctcgcac gaacttctag aaatgccaga gattagggca
    21841 gcttatatgg ggatgtaaat gatttgatgt cagtggctgg cagaatgtgg gataaccata
    21901 aatgcaaaag actatgagat ccgcttgctg gtgatattat tgcaaagact agcaaggctc
    21961 taattggttc tagaataagg attaagcctt attagctcgt gtgatttttt aggtccaatt
    22021 tcccaatgtt gaaagacgat cagaaattca aatattacga tttccgattt ggtaagataa
    22081 tgttcttttt cagccttgta cgtaagtcgt atctggatcg attggatata ttggcctatg
    22141 tattcgagta aaggggatgg attccaaca
//
LOCUS       DAOMZL010000026        20780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_2231_length_20780_cov_17.926224, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000026 DAOMZL010000000
VERSION     DAOMZL010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 20780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20780
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2231_length_20780_cov_17.926224"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..269
                     /locus_tag="PLQ30_03650"
     CDS             18..269
                     /locus_tag="PLQ30_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPW01569.1"
                     /translation="MKNRAGIHARPSALIAQTAIKFASRIYLEKEGNQINAKSIMGII
                     TLAASFGAKIKIIADGADEQEAVKAIAELFESGFNEELG"
     gene            360..962
                     /gene="lexA"
                     /locus_tag="PLQ30_03655"
     CDS             360..962
                     /gene="lexA"
                     /locus_tag="PLQ30_03655"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPW01570.1"
                     /translation="MKDLTDRQKLILSFIREYIQKNNYPPTVRETARAFSMSVKGAYD
                     HIKALEKKGYIKTTEKRSRAIELVSPPEDNTAVVKIPLLGEIAAGKPIFADENFEKTI
                     SIPAEFVGNHIPHFALRVNGDSMVGAGILSGDIAIIEQSETARNGDIIVALLDENVTL
                     KRFYIERNRYKLQAENPGYAPIYTQDLKILGRLRGIYRSY"
     gene            967..2007
                     /gene="holA"
                     /locus_tag="PLQ30_03660"
     CDS             967..2007
                     /gene="holA"
                     /locus_tag="PLQ30_03660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPW01571.1"
                     /translation="MAKYPIPPVWILAGPEVGLKGAFIADLIDKARRLGEGEPEVHRM
                     YAGDSSPSEAIELLQNSSLFSPWVIIEFRNADQISLKPEIDTMVKYCGSPAEQALLLL
                     ETENYSVSKAIEKVVPPSCRKTFFELFENELTGWIRRELGKSQLSIDEEALESFLELV
                     PHDTNSLAAACLVLSASFPPGSMLGASDVEAAILRSKPEDAFTLFDKIGQGDLSLSLE
                     VLNSVLDSRRGDANQIIAALVWSFRRLARIGEAVADGERFEDVCIREQARSKTVQKKL
                     RNALGNYEPEDCRRIISALSETEAAIRGLLGSAFDRQLLQLLIISIITQKGKGLPLAG
                     WSERGLYPQFIN"
     gene            2016..3194
                     /locus_tag="PLQ30_03665"
     CDS             2016..3194
                     /locus_tag="PLQ30_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HPW01572.1"
                     /translation="MSQTVRTIQLGERTIILVGTAHISKESIEEAKATIIAEQPQRVC
                     VEIDMGRYQSISQESQWEKLDIIKVLKQGNGFLLLANLALAGFQKRLGLDLGTKPGEE
                     MLAAIQTAEEMGIPWSAIDREIQTTLKRAWGKSNLWNKSKLLASLIESAFSREKLSEE
                     DLEKLKESSELEQMMNELASYMPSVKEVLIDERDRYLATKIFLTTEQKVVAVVGAGHM
                     NGIEAWLEKLSRGEIEADVSDIETLPKKGWFAKSAGWLIPILIIALITIGFFRSGGQA
                     SLAMIERWVLLNGSLAALGSLLCMAHPVTILSSFLLAPLATMNPLLAIGLFAAIIEAF
                     FRKPTVHDAENLADDVTSLKGFYRNRITHILLVFFFSSLGGMIGNFIALPILASRAIG
                     "
     gene            complement(3268..5358)
                     /locus_tag="PLQ30_03670"
     CDS             complement(3268..5358)
                     /locus_tag="PLQ30_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HPW01573.1"
                     /translation="MNSKVELILDRTSGHILKIFFKAKESIECDEPLLELGEIEIKSL
                     CDALGNECQKAEEAAGFSLGSAAEKLSSARKRFVLVNGWQSWSFAGELKGRERPRRAL
                     LRRDLNLFVDHPAERALRLAAQRRPAQRPHTLCQRARRLFHRRDIISHFLLGLRADQL
                     RLMLVSDNVERWNSPRSTDADRAYLRDLSSCSRNESPCPDYLPPVSFILRGDKISVFV
                     YVAGGSFAAGETIARLCVFIAPDYFSLKDKLGSYWGSDRRFDDLSWLYEPKSVSHGMI
                     GGYASWYNHYTAIDEQIIGNDLDAIKANDNFVNSYFLRRGLPAVFQIDDGWEIQVGDW
                     QADPRKFPNGMEKMARRIAEKGLVPGIWLAPFLLMPNSATAKAHPEWLLRDARGKPVK
                     AGWNPNWGGDVWALDISQRDVEDYLLSLIDTIVNVWGYRYLKLDFLYAGLMQGVFSGR
                     KGGAWQHYARIMAHILEFSRASNGEPVAFLSCGAPIESTAPFMPLMRSGADTREHWEW
                     PQLRLIGHQGRPAAKVNLEDSIGRAILDKSLLLCDPDVIFCRTDLTSLKDTEKFLIAM
                     VAAMFGSQLMTSDDPALFTNHAKIDNNPDKLSEAAFTRELIEWYKHIEGKEFGIERNP
                     GRTRGLYDFFSRDGSIFGSINLSDREQLIFAELSEPSELTRLTESSESFESVAPDLSS
                     KFPMPRHSMIIFGL"
     gene            5483..6373
                     /gene="thyX"
                     /locus_tag="PLQ30_03675"
     CDS             5483..6373
                     /gene="thyX"
                     /locus_tag="PLQ30_03675"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPW01574.1"
                     /translation="MAHCIVPEAEAILDKEFPVLDKGFVRLVDYLGGDQRIAQAARVS
                     YGEGTKSYREDAALIDYLWRHEHTSPFEQVVLTFHVKLPIFVARQWIRHRTARVNEIS
                     GRYSIMRDEFFLPYEDALAPQSEDNKQGRSDMPFDKQTASRIQDILKEGSLFSYERYR
                     ELIDMGLAREVARIALPLSLYTELYWQMDLHNLFRFLMLRLDSHAQREIREYAKVILE
                     ITRKVAPAATASFERYTLEAVRFSGPEMRELRQRLMKAESLEDVATKCVESSKEDQTS
                     ELSRKELERFEEKLKSGRQL"
     gene            complement(6461..8977)
                     /locus_tag="PLQ30_03680"
     CDS             complement(6461..8977)
                     /locus_tag="PLQ30_03680"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPW01575.1"
                     /translation="MSQRESEAIQALARDASFWRHIYETQQAGILIQVNGIIQDLNET
                     AVRMLGGRSASEFIGKPSIETLICPEYYDCILDRTRQLRNGRPSVPPIDIRVRQLNGA
                     ELDVLISATSWIEKNDTIIETMLIDIGPLKQRERLLEAISDLASMPLKKEFFSDVNLA
                     ISLILDSMHKLYAEHHNCGFMSFVQHENGFVEEVWGASSESPQLVSSDPIDNVTLRWI
                     IENSRIITKCESCRDRLHKMPIKGDQSWQILFMPVILEDKLEGCAFWIFYTAIDTDKI
                     EADEERRNDFIQAALIALRTFLIQRDNNRKTSELSILNKIALEVGRKTSLQEVADTTL
                     SILEREKNWQPSVIRFKSRFGETLETISYRSSLVKNVEELQKRIHLFDGLIDRPGLGM
                     TGYVIDSGKPIRCMDLPSDPHYVQTDLNIKYGIYAPISIEGKVEGAIGVESKDYAFND
                     SDLRLLQSMSEIVGMAIRSVRLIEVLQERLHWLEILHRINEQIGIETEPYKLYGFLVD
                     SAFEAIQAESVALLIYNPQTDMLEKVAARSWLDRVFEIPLKPDESISGAIFQSGKMRF
                     SPEIREDPFLISRNLSLIPPGCANIGLPVTYEERILGVFHISIKAPLSFTKELVEFAE
                     MFGSYSGIVIGRIQHVRALKEAEKEIKTAYDETLKGWARAIGYRDNETFQHTLRVTEI
                     ALAIGEALSLDEKTLENLRRGAILHDVGKIGIPDNILRKPKAFTKEEEAIMRTHTSFA
                     YELLRPIDFLKDALSVPYCHHERWDGKGYPQGLKGDNIPLLARIFSVADVYDAMTSER
                     PYRHAWTREAALEYIRSQSGKHFDPRIVDVFLGIVDKIKD"
     gene            complement(9157..10035)
                     /locus_tag="PLQ30_03685"
     CDS             complement(9157..10035)
                     /locus_tag="PLQ30_03685"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW01576.1"
                     /translation="MAQRTIGFIGLGVMGAPMAGHIRASGERLLVYSRTKSKAQLVLD
                     AGAEWRDSPKRIAQECDVIFTMVGYPKDVEEIYFGSDGLIENARSGTILVDFTTSRPD
                     LAVRIYESAKVRGLSAIDAPVSGGDIGAKNATLTIMVGGEEETFQKVKPLLEIVGRTV
                     ILQGGPGAGQHTKMANQIAVAGNLLGTVEALIYAKNAGLDPKHALLSIVNGAAQSWQL
                     SNNAPRMLERNFDPGFYSKHFLKDLRIALDSAHAMHIELPMLALSERLFAKMVNDGMG
                     ELGTQAIYLLYERGLA"
     gene            10174..11484
                     /locus_tag="PLQ30_03690"
     CDS             10174..11484
                     /locus_tag="PLQ30_03690"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HPW01577.1"
                     /translation="MQQRLERASLAASRMLSLETEVKNSALACIAQGLRENRDRIFYA
                     NQLDLTAARQNGLAIPLLRRLEFDKKKLDDVLLGIRDILALPDPAGKVLEARLLDDGL
                     ILKRVSCPIGLIAMIFESRPDALVQMASLAVKSGNAIVLKGGSEANESNKVLATIIAR
                     AGEEKGLPSDWLTLLETREEVGELLAFDKYVDLVIPRGSKEFVSKIKAESRIPVLGHS
                     DGICHVYVHEDADPVMAIKIVLDSKIQYPAACNAAEVLLVHAAFAHRSLMALLQALSK
                     AGVMLDVCEKAAQILDKDPGQTRNLHYSVKTDKDWAVEYLDMRMAVKIVYSLDEAIAH
                     INHYGSSHTDAIVCASSEAANRFLKEVDSASVYHNASTRFADGYRYGLGAEVGISTSK
                     IHARGPMGLEGLCTYKWLLEGYGHIVSDYASGERHFVHKELPPN"
     gene            11499..12743
                     /gene="proB"
                     /locus_tag="PLQ30_03695"
     CDS             11499..12743
                     /gene="proB"
                     /locus_tag="PLQ30_03695"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPW01578.1"
                     /translation="MQIHETMDEASARAALARANRIVVKIGTATISRPESSPARLTRL
                     YRSDGKAFLADLPDGAVAKDSGYPSKGIIDTAYIYNVAEQFAELVQAGRQLILVTSGA
                     IGMGARELGLSRRVTEVRMRQACAAIGQPILMEEYRRAFGVFGLVAAQLLVTRDEWDD
                     RTSYLNLRKTVETLLEARAIPVFNENDSVSTAEIGNAFGDNDRLSAYVASKIDAELLI
                     ILSDVDSLYNSDPRENPHAKPIPYVRELSEEHLSFAGGRGSEFSTGGMKTKLAAVAIA
                     RDAGCRVVIAHGRESNVIKRVACGEPIGTLFDATHALKNRIRWLKNSQPRGNLTIDQG
                     ALAAIRERNSLLPAGIVAVEGDFGRDSVVLVNEIVKLISNFSSAELRAVMHKRSEEID
                     VILGDNVPHVVARPEDMAFLDE"
     regulatory      12830..13242
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            13268..14437
                     /locus_tag="PLQ30_03700"
     CDS             13268..14437
                     /locus_tag="PLQ30_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW01579.1"
                     /translation="MRRIAGIFLSLFLIFFPFFDLQQTRSQHLSAQPTSTQQTTYQKA
                     SALHASFSIEQKNGYKLLKAYNLWPGASSTKTYVLYSRGSKAPRDVAADLAIAVPVKR
                     VVLYSTTYIPAIEAIGELDAVVGVDNADYVYSPALRAQIATGKTIETSKNWMPDIERL
                     IMLKPDIIFNYGIGNEWDTFPKMQEAGLPIALIGDWNEQDPIARAQWTVFIAAFFDKE
                     SIAQERMESVIKAYESLKTLASGASKRPKVLVNGPFQGIWSVSGGQSYMARLIKDAGG
                     DYLWADNKGTGALNLSVEAVFERALKADIWLNPVSGAKRLSDVRVMDPRFSALPVLQK
                     GQVWNNDLRCSPGGGNDYFESAVTNPHLVLSDLIAIFQPELLPNHKFNYYKRLTE"
     gene            14455..15594
                     /locus_tag="PLQ30_03705"
     CDS             14455..15594
                     /locus_tag="PLQ30_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPW01580.1"
                     /translation="MSTYKDYNNRINKEMTDNQPNSARQGLLVACLAGSVLLLALLDI
                     ALGSVRIPLLEVLKSLSSKASRPEWTTIVLVFRMPKMLTAAMAGAGLSVAGLILQSIF
                     RNPLAAPDSLGIGAGASIGVAVLMFAGGSLGALGASAQGIGTQGSALLGNLPPLGYTL
                     LIIASSTGSAAVLIIILLISRRFEQVVTVLIMGLLVGYLTSSLVSLLVYFGSPQKVQL
                     YLSWTYGSFGGVRTNELPYLAIAICLGLIITLREIKALNAFLVSERFASTIGIRVRTS
                     RTKLLVAASILAGSITAFCGPIAFLGIAAPHAARLLSRKADHRILLPIAALTGACFAL
                     LADIISSLPTYGSLLPINPILALIGSPIIISMFFRRNKSSMEVSQ"
     gene            15591..16559
                     /locus_tag="PLQ30_03710"
     CDS             15591..16559
                     /locus_tag="PLQ30_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW01581.1"
                     /translation="MNPRHPTRHIPGIPDIIEPEFKKTKGFEGSETTEGGSRETALTG
                     RDEVKSLRPPPIDYSSKEMPKTIMEANDLSIGWRQGSKLNILAQDIKFKAKQGYLIAL
                     VGPNGAGKSTLLKTIAGLQPPCGGSLLLMGKDISRISIEERAQLIACVFPEQIETGFL
                     TVWELVAFGRYPYTNARHRLTEEDRKQVTEALSMVGMQSFANRTVSSLSDGERQKVQI
                     ARAVAQSTALLIFDEPTAFLDAPSRIEVFKLAERLVRQAGKTLLLCTHEVDLALKTAD
                     ELWVIDRAHRFSAGAPFVIAHSGAIGKAFDLPSVAFDVFSGTFRAR"
     gene            complement(16660..17145)
                     /locus_tag="PLQ30_03715"
     CDS             complement(16660..17145)
                     /locus_tag="PLQ30_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HPW01582.1"
                     /translation="MTIDTVKLDFPTNCNVIIGQSHFIKTVEDIAEIMMNSVPGVKFG
                     LAFCEASGPCLVRTEGTDPYLVEVAQKMAMSVRAGHSFYLVIGPGAYPLNVLDRIKTN
                     PETCRIFCATANPVEVVRALTDQGAAILGVVDGFSPKGVEGAEDKIARKAMLREFGYK
                     F"
     gene            complement(17246..18079)
                     /locus_tag="PLQ30_03720"
     CDS             complement(17246..18079)
                     /locus_tag="PLQ30_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPW01583.1"
                     /translation="MQYERFEGSWFLKSNWHLVKTGQSDQSKGVPVPPQEEPPAPEDC
                     VINLPPPDSILRAFEESREQKRAAASFEDTRTSEKPFWGVPERAAVLYHLLNNRRSRR
                     KYTMEPLTIEELSYLLWAIEGVKENRGKFSFRTTPSGGARHPLDIYVFAHKIKGLNVG
                     LYRYLPVEHELVLERQGDDSIALNEALNGQFWNAACVIMWAAVPYRSEWRYGRAAYKL
                     VALDTGHSCQNLYLACESLGLGTCAIGAYDQEKLDAYLGLDGEEMFAIYTAPVGHVAQ
                     P"
     gene            complement(18081..18542)
                     /locus_tag="PLQ30_03725"
     CDS             complement(18081..18542)
                     /locus_tag="PLQ30_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPW01584.1"
                     /translation="MERADEAVSMHVAGSACSQAVFTVFADELGLEPSVAHRISGGFG
                     GGIGRMGLTCGAVTGGIMALSLALGATDSADQDSKLALYQVVAEYMERLKAFKGSIEC
                     RVLLEGVDLWTEEGRDMVKARQLSEIVCNSMIHKAVEEAERILNEKDRFGV"
     gene            complement(18623..20116)
                     /gene="glpK"
                     /locus_tag="PLQ30_03730"
     CDS             complement(18623..20116)
                     /gene="glpK"
                     /locus_tag="PLQ30_03730"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HPW01585.1"
                     /translation="MDRNSTYILALDQGTTTSRAILFNHDGIIISVKQIPFKQIFPKP
                     GWVEHDPEEIWRTQLKAARNAIEAAQIEPTQIAAIGITNQRETTIVWERSTGKPVYNA
                     IVWQCRRSSSICDEFKAQGASDLIRRKTGLVLDPYFSGTKLTWLFRNYPEIKARAERG
                     ELMFGTVDSWLIYNLTEVHATDPSNASRTMLFDINERRWDEELLSLLEVPANILPEVR
                     PSSALFGVTKKDIFGAEIPVAGVAGDQQAALFGHACFEPGDVKNTYGTGCFTLMNTGP
                     FPVESKHNLLATVAWDLGKGYTYALEGSVFIAGAVIQWLRDQMGLLTDSAESERFARM
                     VEDSQGVYIVPAFVGLGAPYWDSEVRGTVVGLTRGTSKAHFIRAALESIAYQSMDLFE
                     AMEKDSGKRLSCIKADGGASQNSFLMQFQADITGTRVVLPEVSETTALGAAYLAGLAI
                     GYWNGLDDVRKNWRMRREFSPRMAEESRVGLVVGWRKAVATARNFKQ"
     gene            complement(20131..20370)
                     /locus_tag="PLQ30_03735"
     CDS             complement(20131..20370)
                     /locus_tag="PLQ30_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01586.1"
                     /translation="MVPLRQNTLGYNRTRLRSVDKSGFDGEFEFGGRSTWYCHTHEDT
                     HQSILVYLDGALRHRIIYKVLETEIIVTILAKRRK"
BASE COUNT         5320 a         5170 c         4955 g         5335 t
ORIGIN      
        1 tagagctaga gacaacaatt aagaatcgtg ccggtatcca tgcccgtcct tctgccctta
       61 ttgcccagac tgcaattaaa ttcgcatctc gcatatacct cgaaaaagaa ggaaatcaga
      121 tcaatgctaa atccataatg ggcataatta ccctcgccgc gagctttggc gccaaaatca
      181 agataatcgc cgatggcgcc gatgaacaag aggccgtaaa ggccatagcg gaacttttcg
      241 aatccggctt caacgaagaa cttggataaa gaagagccca tagaaagccc atagaaatat
      301 ggggcttgcg tgttttgcag ttctgtgttg tatactatac aaagatacag gagtctgcat
      361 tgaaagatct taccgatcga cagaagctaa tcctcagctt catccgggag tatattcaaa
      421 aaaacaacta tccgccaacg gtaagagaaa cggcacgagc cttttcaatg tcggtcaaag
      481 gcgcatacga ccacataaag gctctcgaga aaaaaggtta catcaagacc acagagaaac
      541 gctctcgcgc aatagagttg gtctcgcccc ccgaagacaa tacggctgtg gtgaaaattc
      601 cgctcttagg cgaaattgcg gccggtaagc ccatctttgc agacgaaaac ttcgaaaaaa
      661 ccatctccat tccggctgaa ttcgtaggta atcatattcc tcactttgca cttcgagtaa
      721 atggcgacag catggtaggc gctggaattc tttcggggga tatcgccata atagagcaga
      781 gcgagacggc aaggaatggc gatatcattg tagctcttct agatgaaaac gtaaccctca
      841 agcgattcta tatcgaaagg aaccgctaca agctacaggc agaaaatcca ggctacgctc
      901 caatttatac tcaggatctg aagatacttg gcagacttcg cggtatatac aggagctatt
      961 gatcctatgg caaaatatcc cattcctcct gtttggatac tcgcagggcc cgaagtggga
     1021 ctaaaagggg cttttatagc cgatttgata gataaggcaa ggcggcttgg ggagggtgaa
     1081 cctgaagttc atagaatgta tgctggcgat agctcgccct ccgaagctat agagcttctg
     1141 cagaatagtt ctctcttttc tccatgggtc ataatcgaat tccgaaatgc ggatcagatc
     1201 agtctgaagc cagagataga tacaatggtc aaatattgcg gcagtccggc ggagcaagcg
     1261 ctgctgctgc tcgaaacaga aaactattcg gtctcaaaag ccatagaaaa agtcgtgccg
     1321 ccttcgtgtc gaaaaacctt cttcgagctc ttcgaaaatg aactaaccgg atggatacgg
     1381 cgggaactag gcaaatccca gctttctatc gacgaagagg cccttgaatc cttccttgag
     1441 cttgttccgc atgacaccaa ctctcttgca gcggcctgcc ttgtactaag cgcgagtttc
     1501 cctccaggaa gcatgcttgg cgcttcggat gtcgaggcgg cgattctgcg ctccaagccg
     1561 gaagacgcct ttactctctt cgataagata gggcagggag atttatctct ttctctcgaa
     1621 gtgctcaata gcgttctgga ctcaaggcgc ggcgatgcca atcagataat agccgctcta
     1681 gtttggagtt ttcgcagact ggcgcgcata ggagaagcag ttgccgatgg cgagcgcttc
     1741 gaagatgtat gcatccgaga acaagctcgc tccaaaactg ttcagaaaaa gctccgaaat
     1801 gcccttggca attatgagcc agaagattgc aggcgaatca tatccgccct ttccgaaacc
     1861 gaggctgcca ttcgaggcct attgggatcg gctttcgacc gccaattgct gcagctgctc
     1921 ataatctcta taatcacaca aaaaggaaaa gggctcccgc ttgccggatg gagtgagcga
     1981 ggcttatatc cacaatttat aaattaggac atattatgtc gcagactgtc aggaccattc
     2041 aacttggtga aagaaccatc atactcgttg gtacggctca tatatcgaag gagagtatcg
     2101 aagaagctaa agcgactatc atagcagaac aaccgcagcg cgtttgcgta gagatcgaca
     2161 tggggcgcta tcaatcgatc agccaagaga gccaatggga aaaactcgac atcataaaag
     2221 tactaaagca gggaaacggc tttctattgc tggcaaatct ggcactcgcc ggatttcaga
     2281 agcgcctcgg gttggatctt ggaactaagc ctggagagga aatgcttgcg gcaatccaaa
     2341 ccgccgagga aatgggtatt ccctggtccg ccatagatcg agaaattcaa acaactctca
     2401 agcgagcgtg gggaaaatcc aatctctgga acaagtcaaa actcctggcc agcctgatag
     2461 aatcggcttt ctcgcgtgag aagctgagcg aagaagatct tgaaaaactc aaagagagca
     2521 gcgaactcga gcagatgatg aatgaactcg ccagttatat gccgtcggtc aaggaagtac
     2581 tcatagatga acgagatcgc tatcttgcca caaagatatt tctgaccaca gagcagaaag
     2641 tggtcgctgt ggtcggcgct ggccacatga acggcataga agcttggtta gaaaagctat
     2701 cgcgaggcga aatcgaggct gatgtctccg atatcgaaac ccttccgaag aagggctggt
     2761 ttgccaagtc tgctggctgg cttattccca tcctcataat tgcgctgata accatcggtt
     2821 tttttcgttc cggaggccag gcgagcctgg caatgattga acgctgggtg ctcctgaatg
     2881 gttctttggc agcgcttggt tcactcctat gcatggcaca tccggttaca attctctcct
     2941 cattcttatt ggcaccgctg gcaacaatga atcccttact tgcaatagga ttgtttgcag
     3001 ccataataga agcctttttt cgtaagccca cggtgcacga tgcggagaat ctagccgacg
     3061 atgtgacgag ccttaaaggc ttttatagga atcgtataac ccatattctg ctagtctttt
     3121 tcttttcgag ccttggcgga atgattggga attttatcgc tcttccgatc ctcgcctctc
     3181 gcgcgattgg ttgaaaaata ttcggtcctt ataatgaatg gtcttgcgct tcttctataa
     3241 tgtcttgagc agtcgaaatg gaaaacatta taagccaaag ataatcatcg aatggcgtgg
     3301 cattgggaac ttcgaagaca gatctggcgc tacggattca aatgattcag acgattcggt
     3361 taatcgagtc aattcggaag gctctgataa ttcggcgaag atcaattgct ctctgtcaga
     3421 aaggttgatg ctaccaaata tagatccatc tctgctgaag aaatcataga gtccgcgtgt
     3481 tcggcctgga tttctctcta tgccgaactc cttgccctcg atatgcttgt accactcgat
     3541 caactctctt gtgaatgcgg cctctgagag cttatcaggg ttattgtcta ttttagcatg
     3601 atttgtaaag agcgcgggat catcggaggt catgagctgg gaaccgaaca tcgcagcaac
     3661 cattgcgata aggaattttt ctgtatcctt gaggctggtc aggtctgttc tgcaaaagat
     3721 gacatctgga tcacatagta gaagactctt gtccagaatt gcgcgcccga tgctatcttc
     3781 caggttgact tttgccgcag gccgaccttg atgacctatg agccttagct gtggccattc
     3841 ccaatgctct cgggtatccg cccctgagcg cattagaggc ataaaaggcg cggtggattc
     3901 tatgggtgcg ccgcaggata gaaaagctac gggctcgcca ttcgacgctc tggaaaattc
     3961 taagatatgc gccataatgc gggcatagtg ctgccaagct cctccttttc gaccggaaaa
     4021 gaccccttgc atgagacccg catatagaaa atccaacttc aaatagcgat aaccccagac
     4081 atttacaatc gtatcgatta aagataagag gtaatcttcg acatcgcgtt gcgaaatatc
     4141 tagagcccat acatcgccgc cccaattagg gttccagcct gccttgacag gcttgcctct
     4201 agcatcgcga agcagccatt cgggatgggc ttttgccgtg gccgaattgg gcataagaag
     4261 aaaaggcgcg agccagatgc ctggcacaag gcctttttcg gcaattcgcc gggccatttt
     4321 ttccatgcca ttcggaaatt tgcgaggatc ggcttgccag tcccctacct ggatctccca
     4381 accatcgtcg atctggaata cggcgggcag gcctcgccgc agaaaatagc tgtttacaaa
     4441 attgtcattg gcctttatgg catcgaggtc attcccgata atctgctcat cgatggcagt
     4501 gtagtgattg taccatgatg catagccacc tatcatgcca tgagaaacag attttggctc
     4561 atagagccaa ctcaaatcgt caaaacgacg atcggagccc caatatgagc caagcttatc
     4621 tttaagcgaa aagtagtctg gcgcgataaa cacgcaaagc cgcgcgattg tctcgccggc
     4681 agcaaaggag ccacccgcca cataaacaaa aacactaatc ttatcgcctc gaagtataaa
     4741 actgacggga ggcaaatagt ctgggcaagg gctttcattt ctggagcaac ttgaaagatc
     4801 tcgcagatag gcgcggtcgg catctgtcga gcgagggctg ttccagcgct ccacattgtc
     4861 tgacacgagc ataagtctta gctggtcggc tctgaggccg agcaagaagt gcgagataat
     4921 gtcgcgccta tggaataagc gccgtgcccg ctgacaaagg gtgtgcgggc gctgcgccgg
     4981 gcggcgctgc gccgccaggc gcagcgcccg ctccgctgga tggtcgacaa acaggtttag
     5041 atctcggcgc aacagggcgc ggcgcggcct ctcccttccc ttcagttccc cggcaaatga
     5101 ccaggattgc cagccattga ccagcacgaa tctctttcgc gcggaagaaa gcttttccgc
     5161 ggcactgcct aagctaaacc ctgcggcttc ttccgccttt tggcactcat tgcccagcgc
     5221 atcgcaaagg ctcttgatct caatctcccc cagctccagg agcggctcat cgcactctat
     5281 cgactcttta gccttaaaga agatcttcaa aatgtggccg gaagttctat caagtattag
     5341 ctctaccttc gaattcatgg ggtttatgct atcatagtcc agcaatgttt ggcatatcca
     5401 ttgctagtat atccattgcc agcccgggca atttctttcc aggtctcaat acaacaaatc
     5461 ggatatgtgc tacaatagat ctatggcaca ctgtatagtt ccggaagcag aagcgattct
     5521 agacaaagaa ttccccgttc tcgacaaagg gtttgtccga cttgtcgact atcttggcgg
     5581 agaccagcgc attgcgcaag ccgcccgagt ctcctatggt gagggcacca agagttatcg
     5641 tgaagatgcg gcgctcatag attacctatg gcggcatgag catacaagcc cattcgagca
     5701 ggttgtgctc acctttcatg taaagctacc tatttttgtg gcacgacagt ggattcgcca
     5761 ccgtaccgcg cgcgtcaacg agatttccgg tcgctattcc attatgcgag atgagttttt
     5821 tcttccatat gaagacgcct tggctccaca gagcgaagac aacaagcaag gccgttccga
     5881 catgcctttc gacaaacaaa cagcatcgag gatccaggat attctcaagg aagggtctct
     5941 cttttcatat gagcgctata gggagttgat cgatatgggc cttgcccgcg aagtggctcg
     6001 catagctctg cccctttctc tctataccga attgtattgg cagatggatc tgcataatct
     6061 ttttcgtttt ctcatgttaa ggctagactc ccatgcacag cgtgaaatcc gcgaatatgc
     6121 aaaggtcatt ctcgagataa ccagaaaagt tgcgcctgct gcaacggcga gttttgaacg
     6181 atatacttta gaggctgtgc gattctctgg gcctgagatg agggagcttc gacaaagact
     6241 aatgaaggcc gaatccttag aagatgtcgc gacaaaatgc gtggaatcga gcaaagaaga
     6301 ccagactagc gagctatcta ggaaggaact cgagcgcttt gaagagaagc tcaaatctgg
     6361 tcgccagctc taggtaacat atttattagt gctacaaacc ttgtgtacga caatataaac
     6421 aaggttcgta tcagtatacg gagctccgct atattcttgc tcaatccttt atcttgtcta
     6481 cgattcccaa aaaaacatcg acgatccggg gatcgaaatg tttcccagat tgcgatcgga
     6541 tatattctaa agccgcctcg cgagtccaag catgacgata aggcctttcg cttgtcattg
     6601 catcgtagac gtctgctact gagaatatcc ttgcaagtaa aggaatatta tcgcctttta
     6661 gaccctgcgg atatcctttg ccatcccagc gctcgtgatg gcaatatggt acgctaagcg
     6721 catctttcaa gaagtctata ggccgcagga gctcgtacgc aaaagatgta tgggtacgca
     6781 tgattgcttc ttcttccttg gtgaatgcct tgggttttcg tagaatgttg tccggtatgc
     6841 cgattttccc tacatcgtga agtatggcgc cacgtcttag gttttcgagt gtcttctcat
     6901 cgagggaaag agcttcgcct attgcaaggg ctatttctgt tacccgcagt gtatgctgga
     6961 aggtttcatt atccctatag ccgatcgcgc gagcccagcc cttcaaggtc tcatcgtaag
     7021 ctgtcttgat ttccttctcc gcttccttca gagctcggac atgttgtata cgcccaatga
     7081 caatacctga gtatgatccg aacatttcag caaattcgac gagctccttg gtaaaggata
     7141 aaggcgcctt gatcgaaata tgaaagactc ccaatattct ttcttcatat gttacgggca
     7201 atcctatatt agcgcatcct ggcgggatta acgaaaggtt cctcgatatg aggaaaggat
     7261 cttctctaat ttcaggcgaa aaacgcatct ttccactttg gaatatggct ccgcttatgc
     7321 tctcgtctgg ctttagcggt atttcaaaga ccctatcgag ccaagacctg gccgcaacct
     7381 tttccaacat gtctgtctgt ggattataga ttagtagggc aacggattca gcctgtattg
     7441 cttcgaatgc gctatcgaca aggaatccgt agagcttgta tggttccgtc tcgattccaa
     7501 tctgttcatt tattctatgt agtatctcaa gccaatgcag acgttcttgg aggacttcta
     7561 tgagccttac agaccttata gccataccga caatctcgct catagactgc aggaggcgca
     7621 ggtcagaatc attgaaagcg taatctttgc tctctactcc tattgctccc tccaccttgc
     7681 cttcgatcga tatgggagca tatatgccat acttgatatt cagatccgtc tgaacataat
     7741 gcggatcaga gggtaaatcc atacaccgta taggctttcc gctatcaatg acatagcctg
     7801 tcatacccag accaggacgg tcgatcaatc catcaaagag atgtatcctc ttttgcaatt
     7861 cttcgacgtt cttaacaagc gaactcctgt acgaaatcgt ctcaagagtc tcgccgaatc
     7921 tggatttgaa tcttattacc gaaggctgcc aattcttttc tctttcgaga atgctgagtg
     7981 tagtgtctgc aacctcctga aggctcgtct ttctgccaac ctcaagagct attttgttga
     8041 ggatggagag ctccgaggtc ttccggttat tatcgcgctg tatgagaaat gttctcaaag
     8101 ctatcaaggc agcttgaata aagtcatttc gtcgctcttc atctgcttcg attttatcag
     8161 tgtctattgc cgtgtagaaa atccaaaacg cacagccttc cagtttgtcc tctaggataa
     8221 caggcataaa aaggatctgc caggattgat ctcctttgat gggcatctta tgcaatctgt
     8281 ctctgcatga ttcgcatttt gtaataatcc ttgaattctc gataatccac ctcagcgtaa
     8341 cattatcgat tggatctgag gatacaagct gaggggattc acttgaggct ccccaaactt
     8401 cctcaacaaa accattttca tgctgcacaa agctcatgaa gccgcagttg tgatgttccg
     8461 catatagctt atgcatagaa tccaggatga gcgatatagc aaggtttaca tccgagaaga
     8521 attctttctt taacggcata gatgccaagt cagagatcgc ctctagcagc ctttcgcgct
     8581 gctttaatgg tccaatatcg atcagcatag tctcgattat tgtatcgttc ttttcgatcc
     8641 atgaagttgc cgatattagt acatcaagtt ccgctccatt tagctgtcgc actctgatat
     8701 ctatgggtgg aaccgaagga cgaccatttc gcagttggcg cgttctatcg agaatgcaat
     8761 cgtaatactc ggggcatatc agtgtttcaa tagaaggttt gccgatgaat tctgaagcgg
     8821 accgtcctcc taacattctg acagcagtct cattcaggtc ctgaattatt ccattgacct
     8881 gaatcaatat gccggcttgc tgggtttcgt agatgtgtcg ccagaacgaa gcatcccttg
     8941 caagagcctg tatggcttct gattctcttt gactcatttt ttatatcact gcctgtttcg
     9001 accctttggg tcgatgtttc accccctttg ggggcaatta tttattctaa tgtccagcat
     9061 agagcttcaa caatgtttgc tatatcggca caaaaatcat aaaaatcaat ctattatcgc
     9121 cgatatcgaa gaaatcataa aggcaatatt gcatttttat gctaatccgc gctcatagag
     9181 cagatatatt gcctgcgtgc ccagctctcc cattccgtcg ttcaccatct tggcaaaaag
     9241 gcgttcggaa agagccaaca ttggcaattc tatatgcatt gcatgagcgg aatccaaagc
     9301 tatgcgcaga tccttgagaa aatgcttact atagaagcct ggatcgaaat ttctctcaag
     9361 cattcgtggt gcattgttag agagctgcca gctttgcgcg gcgccattca caatcgataa
     9421 gagcgcatgt ttgggatcga ggcctgcgtt ttttgcatag ataagagcct ctactgtgcc
     9481 aagaaggttg cctgccacgg ctatttggtt ggccattttt gtatgctgac cagcaccagg
     9541 accgccttgc aggatgacag tcctgcccac tatttcaagg aggggtttta ctttctggaa
     9601 tgtctcttcc tcgccgccga ccatgatggt caaagtagca ttctttgcgc cgatatcccc
     9661 accagaaaca ggcgcatcga ttgcgctcag tccgcgaact tttgccgatt cgtagattcg
     9721 aaccgcaaga tcaggtctcg atgtggtgaa atcgacgaga atagtgccag atctagcatt
     9781 ctcaataagg ccatccgagc cgaagtatat ctcttccaca tcttttggat agccaaccat
     9841 agtaaagatc acatcgcatt cctgggcaat tcgtttggga gaatctcgcc attcggcccc
     9901 tgcgtctaat acgagctgtg ccttgctttt cgtacgcgaa tagaccaaaa gcctttcgcc
     9961 ggaagcgcga atatgtccgg ccatgggagc ccccatcaca cccagtccaa tgaatccaat
    10021 tgttctttgc gccatttctc ggtctccttt gcaatttaga tgttcaatct tcgcgaggaa
    10081 ttattcaatt ccacctggtt catcttgtca aatataatac atttccacct ctttttgatt
    10141 tcattgattt tttgtatagt atgatgaatg aatatgcagc aaagacttga gcgggcaagt
    10201 ctggccgctt ctagaatgct ctccttagaa acagaagtca agaattctgc gcttgcctgc
    10261 attgcacaag gcttgcgaga aaatcgcgat aggattttct atgcaaatca attggacctg
    10321 accgccgcca ggcaaaacgg attagctata ccgcttttaa ggcgccttga attcgacaaa
    10381 aaaaaattgg atgacgttct tttgggcatt cgggatattc ttgcattgcc agatcctgca
    10441 ggaaaagtgt tggaagcgcg ccttttggat gatggcctga tattgaaaag ggttagctgc
    10501 ccgattggac tcatagcgat gatcttcgaa agccgccccg atgcccttgt gcagatggct
    10561 tcattggccg tgaagagtgg aaacgccatc gtgctgaaag gcggcagcga ggcaaatgaa
    10621 agcaacaagg ttctggccac tataattgcc agggccggcg aagaaaaagg ccttccttcg
    10681 gactggctca ctctcctgga aacacgcgaa gaagtgggcg aactgctagc attcgataaa
    10741 tatgtcgatt tggtcattcc tcgcggttcc aaggaattcg tttcaaagat aaaggctgaa
    10801 agccgaatac cggtgcttgg gcattcggat ggtatctgcc atgtctatgt gcacgaagat
    10861 gcagacccgg tgatggctat caaaatcgtt ttggactcga agatacagta tccagctgcc
    10921 tgcaatgcgg cggaagtact tcttgtgcat gccgcatttg cgcatcgcag ccttatggcg
    10981 cttttgcaag ctctttccaa agccggagtg atgctcgatg tctgcgaaaa agcagcccag
    11041 atactcgata aggatcccgg gcaaacccgg aatctgcact attctgtaaa gaccgataaa
    11101 gattgggctg tcgaatacct ggacatgcga atggctgtca agattgtata ttccctcgat
    11161 gaggcaattg cgcacataaa ccattatggg agcagccaca ctgatgcgat agtctgtgct
    11221 tcttccgaag cggcaaatcg cttcctcaaa gaagtggatt ccgcttcggt ctatcacaat
    11281 gccagcaccc gatttgcgga tggctatcgc tatggcctgg gcgcagaagt cggtatttca
    11341 accagcaaga tccacgccag aggcccaatg ggacttgaag gactttgtac ttacaaatgg
    11401 ctgctcgaag gctatgggca tatcgtcagt gactatgctt caggcgaacg ccactttgtg
    11461 cataaagaac ttcccccgaa ttgagagagg aaaataccgt gcaaatccac gaaactatgg
    11521 atgaagcctc tgctcgcgca gcgcttgcgc gcgctaatcg tatcgtagtc aaaattggta
    11581 ccgcgaccat ttccagacct gaatctagcc cggctcgcct gactcggcta tatcgatccg
    11641 atggcaaggc ttttttggcc gatttgcccg atggggctgt cgcaaaagac tcaggatatc
    11701 cttcgaaagg aattatcgat accgcatata tctataatgt agcagaacag tttgccgagc
    11761 tcgttcaagc cggtaggcag ctaatccttg tcacctcggg tgcaatcggc atgggtgcac
    11821 gcgagctggg cctttctagg agagtcaccg aagtgcgcat gcgtcaggct tgtgccgcaa
    11881 taggtcagcc catacttatg gaagaataca ggagggcgtt tggcgttttt ggcctggtag
    11941 ccgcccagct ccttgtgact cgagatgagt gggatgatcg cacgagctac ctcaacctcc
    12001 gcaagacagt tgagactctc ttggaagccc gggcaatacc tgtcttcaac gagaatgatt
    12061 cggtctctac ggcagagatc ggcaatgcct tcggcgacaa tgacaggctc tcggcctatg
    12121 ttgcgagcaa gatcgatgcg gaattgctca ttatcctttc cgatgtggat tcgctctaca
    12181 attcagatcc aagagaaaat ccccacgcca agcccatccc ttatgtgcga gagctatcgg
    12241 aagagcatct ttcgtttgcc ggtggtcgag gttcggaatt ttctacagga ggaatgaaga
    12301 caaagcttgc cgccgttgcc attgcacgcg atgcaggctg ccgcgttgtt attgctcatg
    12361 gtcgcgagtc caatgtaatt aagcgcgtcg catgtggtga accgatcgga acacttttcg
    12421 atgcaaccca cgcacttaaa aatagaattc gatggctcaa gaattctcaa cctcgcggca
    12481 atttaacaat agatcaaggt gctctagcgg cgatacgaga gcgcaattcc ctgctgccgg
    12541 ctggcatagt tgcggtggaa ggcgattttg gccgcgattc tgtggtattg gtcaatgaaa
    12601 tagtaaagct aataagcaat ttttcttcgg ctgagcttag ggctgtcatg cataaaagaa
    12661 gcgaagagat agatgtgatc ctgggcgata atgtgcccca tgtcgtcgct agacccgaag
    12721 atatggcttt cttggatgaa tgattccttc atgcatcgat tttggtagag catatagaat
    12781 ggcacacaga ccaaagacgc ggccttttct tattctgccc attgtagtaa aataaaccaa
    12841 gctcatgggt gccgtttgtg cggcgtcgcc gcgcggcgga aaagggaaaa cggtggaaaa
    12901 ccgttgcagc ccccgctact gtgagttggt atagatgcgg tccagcgata tcgatattgt
    12961 acaatatcgc tgcagccaga gctaagatcg ctatcacaat agatgcttga tcttgttcgc
    13021 tttggctgat ccactggttc cgacgctttt gttcgacttc tgatcgattg gcttccgatc
    13081 ggaaagatat tgatagaagc agaacaaaag ccaccagaac tgggaaggag aaccacatcg
    13141 tatgcatttc tagcttttct tatgctgaga tttgagcatt atagaactga gaaattgcat
    13201 agccggcaag ccaggaaacc tgcccttgaa catttccatc cttaatcttc ggtgggagga
    13261 ggcggatatg cgacgaatcg ccggtatttt cctttctcta tttctgattt tttttccatt
    13321 cttcgacctc cagcaaacaa ggtcacaaca tttaagtgcc cagccaacaa gcactcagca
    13381 aaccacatat caaaaagcca gcgcactaca tgcaagcttc agcatagagc aaaaaaatgg
    13441 ctacaaacta ttaaaggcgt acaatctctg gccgggagct tcttctacaa aaacatatgt
    13501 gctctactcc agaggatcta aagctcctcg agatgtcgct gcggatctcg ccatagcagt
    13561 gcctgtgaag cgcgtcgttc tctattctac cacctacatc cctgctatcg aggcaatcgg
    13621 agagctcgat gcagttgtgg gtgtcgacaa tgccgattac gtatatagtc ccgccctgcg
    13681 tgcacagata gcgacaggca agactatcga gacctcaaag aattggatgc ccgatataga
    13741 acggctgatc atgctaaagc ctgatatcat attcaattat ggcattggca acgaatggga
    13801 tactttccca aagatgcagg aggcagggct gcctatcgca ctgattggag attggaacga
    13861 acaggatcca atagcaaggg ctcaatggac cgtgttcatc gcggctttct ttgacaagga
    13921 gagcatagcc caagaacgca tggaatcggt gataaaagcc tatgagagcc tcaaaacgct
    13981 cgcatcagga gcctctaagc gaccaaaagt cctggtaaac ggtcctttcc aaggcatatg
    14041 gagcgtctcg ggtgggcaga gctatatggc aaggctcata aaggatgcag gtggcgatta
    14101 tctttgggca gacaataaag gcacgggagc tctcaatctt tcggtggagg cagttttcga
    14161 aagagcacta aaggcggata tatggctcaa ccctgtctcc ggagcgaagc gactttccga
    14221 tgtgcgggtt atggaccctc gcttttctgc actgcctgtt ctgcagaaag gacaggtttg
    14281 gaacaatgat ctcaggtgtt ctcctggagg aggcaacgat tacttcgagt ccgcggtaac
    14341 gaatcctcat cttgttcttt ctgacttgat tgcgattttt caaccggagc ttcttccgaa
    14401 ccataagttc aattattaca aaagattaac tgaatagagc ctgagtactg ctgcatgagt
    14461 acctataagg actataataa tagaatcaat aaagaaatga cagacaatca gcctaatagc
    14521 gcacggcagg gattgctcgt tgcatgcctt gcaggttctg ttctgctact tgctctgctg
    14581 gacatagcat tgggttcggt acgcatcccc cttttggaag tgctaaagtc cttaagcagt
    14641 aaagcatcgc ggccggaatg gactactatc gtccttgtat ttcgaatgcc caagatgcta
    14701 acagcggcta tggcgggagc agggctctcg gttgcggggc tcatcctgca atccattttt
    14761 cgaaatcctt tggcagcgcc ggattctctt ggtattggag ctggcgcatc tattggagtg
    14821 gcggtactaa tgttcgcagg aggatctctg ggagctcttg gggctagcgc acagggaata
    14881 ggaactcaag gcagtgcgct cttaggaaat ctgccgcccc tgggatatac actgcttata
    14941 attgcgtcca gcactgggtc cgcggccgtg cttatcatta ttcttctaat atcgagaaga
    15001 tttgaacagg ttgtgaccgt gctgataatg ggtctgctag tgggttatct tacatcgagc
    15061 cttgtgtcac tgcttgtata ttttggttct cctcaaaaag ttcaactcta tctttcgtgg
    15121 acctatggct cctttggagg tgtgcgaaca aatgagctgc cttatttagc gatagctatc
    15181 tgccttggat tgatcataac cctgcgcgag ataaaggcac tcaacgcctt tctggtgagc
    15241 gagcgctttg cttcgacaat tggcattagg gttcgaactt caagaacaaa gctcttggtg
    15301 gctgcttcaa tcctggctgg aagtatcacc gctttctgtg gacccatcgc attcttgggt
    15361 atagccgctc cccatgctgc tcgcctacta tcgcgcaagg ccgatcatcg cattctactg
    15421 cctattgctg cgcttacggg agcttgtttc gctctcctgg ccgacataat atcttcgctt
    15481 cccacttatg gctcgctatt gcctataaat ccaatcttgg ccttgatagg ttctccaatc
    15541 atcatttcga tgttttttcg caggaataag agctcgatgg aggtcagcca atgaatccaa
    15601 gacatcccac gcgccacatt cctggtattc ccgatatcat tgagcccgag tttaagaaaa
    15661 caaaaggatt cgaaggctct gaaacgacag aaggaggatc gagagaaaca gcgttgacag
    15721 gaagagatga agtgaaaagt ctaagacctc ctcccatcga ttattcttcc aaagagatgc
    15781 ctaaaacgat tatggaagcc aatgacttat ctattggctg gaggcaaggc agcaaactca
    15841 atattctggc acaagatatc aaattcaagg caaaacaggg atatttgatc gctcttgtcg
    15901 gcccaaatgg agcaggcaag tctaccctat taaaaaccat agcaggactc cagcccccct
    15961 gtgggggttc tctattattg atgggcaaag atatttccag gatttcgatc gaagaacgag
    16021 cgcagcttat tgcctgtgtt ttccccgaac aaatcgagac cggttttctc accgtgtggg
    16081 aactggtagc ctttggtcga tatccttaca caaatgcacg tcatcgcctg acggaagagg
    16141 atagaaaaca ggtcacagaa gcgctttcga tggtcggaat gcagagtttt gcaaaccgaa
    16201 cggtgtcgag cctctccgac ggagagcgac aaaaagtgca gatagcccgg gcggtagcgc
    16261 agagcacggc gctattgatc ttcgacgagc caaccgcctt tctagatgct ccttctcgca
    16321 ttgaagtctt caagcttgca gaacgtcttg tgcggcaggc tggaaagact cttctgctct
    16381 gtacacatga agtggatctt gcccttaaga ccgcggatga gctttgggtc atcgatcgcg
    16441 cgcatcgttt ttctgccgga gctccctttg tgattgcgca ttctggagcg ataggaaaag
    16501 cctttgacct gccatcggtt gcttttgatg tctttagtgg cacattcaga gcccgatagc
    16561 gcacttcatc caggtcatcc tttcgacccc cctcgatccg gctttattca gtcatggtat
    16621 acaaatatcc tgtctatata ctttcattaa ggcaagaatt cagaacttat agccaaactc
    16681 tcgcagcatg gcctttcggg caatcttatc ttctgcaccc tctacacctt taggcgaaaa
    16741 accatcgacc acgccgagga ttgcggctcc ttgatccgta agtgccctca ctacttcgac
    16801 aggattggcc gttgcacaga aaatcctgca ggtctcgggg tttgtcttta tgcggtccag
    16861 cacatttagc ggatacgcgc cgggaccgat tacaagataa aaactatgcc ctgcgcgcac
    16921 tgacatggcc atcttttgag ccacctcgac cagatatgga tccgtgcctt ctgtacgaac
    16981 gaggcatggt ccggaagcct cacaaaaggc aagaccgaac tttactccgg gcaccgagtt
    17041 catcattatc tccgcaatgt cttcgactgt ttttatgaaa tgcgactggc cgattatcac
    17101 attgcaattt gttggaaaat ccagcttgac tgtatcgatg gtcatgatca aaacctcctt
    17161 gggctaatct tctatactga tacgaacctt gtttatattg tcgtacacaa ggttcgtagc
    17221 actaatcaag acaaaagcta ttattttatg gctgagccac atgcccaacg ggcgcagtat
    17281 agattgcaaa catctcttct ccatcgagtc cgagataagc atcgagtttc tcttggtcat
    17341 aagcgcctat cgcacaggtt ccgagaccga gcgattcgca ggcaagatag aggttttggc
    17401 atgaatggcc cgtatctaga gctacaagtt tatatgcagc cctgccatag cgccactcgc
    17461 tgcgataggg aacagcagcc cacattatca cgcatgcggc attccagaac tgcccattca
    17521 atgcttcatt cagagctatc gaatcatctc cctgtcgctc gaggacgagc tcatgttcga
    17581 ctggaagata acgatataga cccacattca agcctttaat cttgtgagca aagacatata
    17641 tgtcgagagg gtggcgcgcg ccgccggagg gagtggtgcg aaaagaaaac ttgccgcgat
    17701 tctcttttac gccttcgatt gcccacagga gataagagag ctcttcgatc gttagcggct
    17761 ccattgtata tttgcggcgc gatctgcgat tattgaggag gtggtagagc acggcggcgc
    17821 gctcaggcac accccaaaaa ggtttttcag aagtcctcgt gtcttcgaat gaggctgcag
    17881 ctcgcttctg ttcccggctc tcttcaaaag cccttagaat gctatcggga ggcggcaggt
    17941 tgatcacaca gtcttcgggc gcaggtggtt cctcttgtgg aggaacaggt actccctttg
    18001 actgatccga ttgccctgtc tttactaaat gccagttgga ttttagaaac caacttcctt
    18061 cgaaacgctc gtattgcata tcaaactcca aatctgtcct tttcgttcaa aattctctct
    18121 gcttcttcaa cggccttatg gatcatcgaa ttacagacta tttcggagag ctgcctggct
    18181 ttgaccatat cccgaccttc ctcagtccat agatctacgc cctccaaaag gaccctgcat
    18241 tcgatgctgc ccttgaatgc cttaagcctc tccatgtatt ccgcaacgac ctgataaagc
    18301 gctaatttac tatcctgatc agccgaatca gttgctccaa gagcaagcga aagagccatg
    18361 attccacctg taacagcacc gcaggtcaaa cccatgcgcc ctattcctcc tccaaatccc
    18421 ccagaaatac gatgagctac ggaaggctct aagcctagct catccgcaaa tacggtaaac
    18481 acagcttgag aacaggccga tccagccacg tgcatcgata ctgcttcatc cgccctttcc
    18541 atttccatcc tccgattata atctgcactt actctgtcat tgtatatgtg attgcatatg
    18601 tcatggctac tgtcaaaggt acttattgct taaaattcct cgctgtagct actgccttgc
    18661 gccaacctac cacaagaccg acccttgatt cttctgccat tcgcgggctg aattccctgc
    18721 gcatgcgcca gttcttgcgc acgtcatcga gcccattcca atagcctatc gcgagaccgg
    18781 caagataggc cgctccaagc gccgttgtct cgctcacttc tggaagaacg accctggttc
    18841 ctgtaatatc ggcttgaaat tgcatgagaa atgaattttg ggaagctcct ccatcggcct
    18901 ttatgcaaga aaggcgtttc cccgaatcct tttccatagc ttcaaatagg tccatggatt
    18961 gataggcgat agattccagc gctgcacgaa tgaaatgcgc cttcgatgtt cctcgcgtaa
    19021 gccctactac agttccgcgc acctccgaat cccagtaagg tgcacccagg cccacaaagg
    19081 ccggaacaat atagactccc tgactatctt ctaccatacg agcaaatcgt tcactttccg
    19141 cagagtccgt taatagaccc atttgatctc tcaaccactg gatcaccgct ccagctatga
    19201 aaaccgaacc ttctagagca taggtgtaac ctttgccaag atcccacgca accgttgcga
    19261 gcagattatg cttagattca acggggaaag gtcctgtatt catcaaggtg aagcagcccg
    19321 taccataggt gttcttcaca tctcccggct cgaagcacgc atgcccgaaa agtgctgctt
    19381 gctggtcgcc ggcaactcca gctaccggaa tttcggcgcc aaatatatcc ttcttcgtaa
    19441 caccgaagag agccgacgaa ggccttacct caggcagaat attagcgggt acttcaagaa
    19501 ggcttaaaag ctcctcatcc caacggcgtt cgtttatgtc gaatagcatt gtgcggcttg
    19561 cgttggaagg atcggtcgca tggacttctg tcagattata gataagccag gaatcgacag
    19621 tgccgaacat cagctcgcct cgctctgcgc gcgcctttat ttcaggatag tttcggaaga
    19681 gccatgtgag ctttgtgcca gagaaatacg gatcaagcac tagacctgtt tttcgtctga
    19741 tcaaatcgct tgcaccctgc gctttgaatt catcacagat agagctcgat cgacggcatt
    19801 gccaaacaat tgcattatag acaggcttac ccgtggaacg ctcccacaca atagtcgttt
    19861 cgcgctgatt ggtaatacct atcgcggcaa tctgtgttgg ttcaatctgc gctgcctcaa
    19921 tcgcgtttct cgctgccttt agctgcgtgc gccaaatctc ctcagggtcg tgttcaaccc
    19981 atcctggctt gggaaaaatc tgtttgaatg gaatctgctt tacggagatg ataattccat
    20041 catgattgaa caaaatagcc ctcgaggtcg ttgtgccctg atccagggca agaatatagg
    20101 ttgaattgcg gtccatgcga agattatagc ctattttcga cgcttcgcaa gaatcgtgac
    20161 aataatctct gtttcaagaa ctttgtagat gattctatgc cgcaatgctc catcgaggta
    20221 gacaaggatg ctctgatggg tatcttcatg ggtatggcaa taccaggtag atcggccacc
    20281 aaactcgaat tcgccatcga atcctgattt atcgacagac cggagccgcg ttcggttata
    20341 gccaagggta ttctggcgta agggcaccat aagggcaccg catagcgcaa tcctacatta
    20401 tccaaagtac gcaggcattc cttccgaact tcctctgtaa aacgcataga ggaggcttat
    20461 ctctgggtca caacagtgct cgttagctca ggcgctcagg gaagaggccg agaatggcgc
    20521 ggtcaagaaa cttattgctt attgactact cttggcttgc cgaagaaggc caggaaaaag
    20581 ctgacgtctt tcagatttag aaaacgacac cgcgggctag ctgaattcgc cgcgtggtgc
    20641 taccctcgcc ctatgccgcg aaaatatgaa ttcggactca ccccctgggg agcatatttt
    20701 atacgagcca tggagagcct tgccgatcag gcgcgcctta agcgtggacg aagttacgcg
    20761 gccaatggga atgtgttcag
//
LOCUS       DAOMZL010000027        19954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_2376_length_19954_cov_17.661239, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000027 DAOMZL010000000
VERSION     DAOMZL010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 19954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19954
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2376_length_19954_cov_17.661239"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PLQ30_03740"
     CDS             <1..846
                     /locus_tag="PLQ30_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPW01587.1"
                     /translation="ISAFCNAGFSTLRSGMESFRDNVLLNVVTMLLIVSGGIGFIVLQ
                     DIMRFVKKEKRRLSYHSSVVLRTTAVLIVLGAIVFYVLEQNHAYKEMSQGSKVMAALF
                     QSVTPRTCGFDTVSQANLSSSSQLVTIILMFIGASPGSTGGGIKTVTLYILLLVALRM
                     REGGDTIVDRYRTVTHNAVLKAITALVRGATIVLVATTILLVSERAAGHTVGLETAIF
                     ECVSAFGTVGLSIGITPYLTAISKLVLIGAMFMGRAGLFILAIPPSRYSPEPFAKYPK
                     ADLLL"
     gene            872..1639
                     /locus_tag="PLQ30_03745"
     CDS             872..1639
                     /locus_tag="PLQ30_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPW01588.1"
                     /translation="MKQFAFIGLGPFAMSMLERISEISGQIIAADESPERIEHVKEMV
                     SVAFTMNLLDEEAFERAFHEPVDVAIVDIESNGPELLLVTFRLKKLGVPEIIVKSNSE
                     EYEEILRLVGATRVVNSDREAALRVTPLVLSSSLTNFMPIGGDLVLAEVIAPDFVVGK
                     SVIEGDLRNTRHINVVAVKYASEMDSSGSQSAIFRNLDTKYRFKDGDVLLVTGKENDV
                     FEFSGIQKTIEHEKERAKTISFLKGMFSRKKKDVPKK"
     gene            1805..2983
                     /locus_tag="PLQ30_03750"
     CDS             1805..2983
                     /locus_tag="PLQ30_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW01589.1"
                     /translation="MDIFQKCFDFDLDKQAMEKGLYPYFRPLDGLEGTEAVFNGRKLI
                     MIGSNNYLGLTMHPKVKEAARKGLEEFGPSCTGSRFLNGTLKLHNELEERLATFVGKE
                     AALVFSTGMQTNLGAISSLVDRNDVVIIDKDDHASIVDGCRLSMGEMKRYLHADLDHL
                     ERILQKVPSESGTMVVVDGVFSMGGDIVDLPGLVRLCQKYGARLYVDDAHSIGVLGDG
                     HGTAWHFGLTDKVDIIMGTFSKSFASLGGFVAGDRDVINYIKHTARSFMFSASLPAPN
                     TMAALAALDIMETEPEHVKHLWDNANFMMKGFRELGFNIGKTQTPIIPVIIGEDETCF
                     RFWKELFDEGLYTNPVISPAVPEGMALLRTSYMATHTREQLQRALDIFEKAGRKFGII
                     "
     gene            complement(3209..3916)
                     /gene="radC"
                     /locus_tag="PLQ30_03755"
     CDS             complement(3209..3916)
                     /gene="radC"
                     /locus_tag="PLQ30_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPW01590.1"
                     /translation="MKEWQAYEKMGQKFESNSSSLDIRERMLKLGSGSISNLELIVAI
                     LGSGVPGKPVRKLAKEVLNQITKSSDSCDLDQLMLISGMGEAKSCAVAAALELGRRIF
                     STRGTRIAMPKDAYPLLIHFADIRQEHFIVISLNGGHEVNAIREITKGLINKTVVHPR
                     EVFADPITDRACAVVVAHNHPSGNLEPSDEDVDITKRLRQSGDILGIPVLDHLIFSES
                     GYFSFMEHGLIVPTKGN"
     gene            4272..4847
                     /locus_tag="PLQ30_03760"
     CDS             4272..4847
                     /locus_tag="PLQ30_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPW01591.1"
                     /translation="MKSIKGTKTEANLLTGFAGESQARNRYTYFASKAKEEGYIQIQL
                     IFEETANQEKEHAKRLFKFLEGGTAKVSAEFPAGVIGTTRENLDEAAGGENYEWQEMY
                     PTFAKVARDEGFEAIASVFDSIAVAEKQHARRYEALMTNIDEGRVFKREEPVIWRCIN
                     CGYVYEGTEPPKACPACAHPQDYFELLAENW"
     gene            4866..5240
                     /locus_tag="PLQ30_03765"
     CDS             4866..5240
                     /locus_tag="PLQ30_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HPW01592.1"
                     /translation="MTERKQVYKCMKCGNIVEVLHAGQGQLVCCGEPMRLMVENTSDG
                     AKEKHVPVLEKTADGWWVMVGSILHPMEEKHYIEWIELISDENSYRVYLAPGQEPKAF
                     FPVKAEKVQAREYCNLHGLWKT"
     gene            5268..5429
                     /locus_tag="PLQ30_03770"
     CDS             5268..5429
                     /locus_tag="PLQ30_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPW01593.1"
                     /translation="MNKYVCDVCGYIYDPAEGDPEGKIPAGTPFEQLPDDWVCPMCGA
                     SKDSFSPMD"
     gene            5568..5858
                     /locus_tag="PLQ30_03775"
     CDS             5568..5858
                     /locus_tag="PLQ30_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01594.1"
                     /translation="MISRDDFVFCVGYDGDTAIIDGKAKKEFCGLSTMELAEKGLYRA
                     AFASALYSQNPEEMKVFIDFFNKKAGTAYTEAGQLSRLFGVYLETISKVKAL"
     gene            5953..7395
                     /gene="radA"
                     /locus_tag="PLQ30_03780"
     CDS             5953..7395
                     /gene="radA"
                     /locus_tag="PLQ30_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPW01595.1"
                     /translation="MKKPSTIYRCSACGHEEPKWLGRCPECGQWNTMVEAKTIGRFEK
                     DKTAFSVPLESVDPALGARVSSGISELDRVLGGGFMRGSAVLLGGEPGIGKSTLLLQA
                     CSKLGAKGKALYISGEESAAQIRLRAERIKALSKQIEVFCGNDMHACLSVMDSAHPLL
                     TVVDSIQTVHSEEAGAVPGTPNQIKFCTQEFVEWAKSHDSIVVLVAHVTKDGMIAGPK
                     AAEHLVDAVISFEQAENALRVLRTSKNRFGSSDELGFFLMSEGGLSELQDPSGIFMIR
                     REGQLPSGIALAIVYEGSRILLAEVQALTIPSKSGIMRVYSDRIDPLRVSRIAAVLEK
                     QTRLDFSSQEIYVNVAGGLRLTEPAVDLPLACALYSARSGQPLPEGSALAGELSLAGE
                     IRPVRSMDRRTRAAHQLGFARIIGPKALPAGAEVPAKRGAAKAEVVGAEAAKSGTVRV
                     EAAALSGQTLPWLGASSLRESLRILWGQGT"
     gene            7397..9613
                     /locus_tag="PLQ30_03785"
     CDS             7397..9613
                     /locus_tag="PLQ30_03785"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPW01596.1"
                     /translation="MQFINNGQRIAGLVVPLLSLKKNENAACGVFSDLVSLGKIAKKW
                     GLSLIQLLPLNDTGNGPSPYAAISAFALHPIYISLSDTIDTMALLGLPFKDEVAKELV
                     YAEKALDRRFGNGGRIQRVQFEAVLQRKIDALRKAWEASRQICSPLAEAFASRESWAK
                     PYACFVALREKFGLAPWWEWPEWQNVLPADIDKLWSNQELAEEERFRLWLQVLAQDQL
                     RHAAQTAHELGIDIMGDIPILLAKDSADVWFQREIFILDKQAGAPPDMYSPRGQNWGF
                     PLYDWNALRARNYDFWRQRLQAAENFYSGYRIDHVLGFFRIWAISIHESDAYLGAFEP
                     SGYLEPAELNSMGFSDERLTWLSKPHIPEWKIQKAEKRCLDAGMPLLAESEASGIENK
                     QKGKREENREDNREDERADIRALFGKRLEELRKSCFVRIKNEPLFLFKPEIRGTADLN
                     ALFGPLWSEFPEAAPIIDEYAGIMRDWWIDRTLYQYEPDRFVFQWSYRETTSWKSLSL
                     QEQQALEAKAKALSEESLNIWEKRGRKLLSMLVSASEMQAFAEDLGAVPPCVPKVLRE
                     LKIPGLRVLRWEREWDKPNQPYIPFDRYEALSISCTSVHDSTSLRQWWQEEADRSQIW
                     AICQALAEHDELLKSLLQRCGDTAPEQLGSKEAAVLVRALALSGSIVAAYPIQDILAC
                     HTEWRLPNAQDERINVPGTTLPTNWMYRMPIDLQALAKDEDFAGFISKVMARGGQA"
     gene            9610..10560
                     /locus_tag="PLQ30_03790"
     CDS             9610..10560
                     /locus_tag="PLQ30_03790"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPW01597.1"
                     /translation="MSNSAQRMQQIAHTTIKRTIDTSLFLIKIMVPVSLLVALLGWSG
                     LLEKISVVLHPIMRLLGLPGEAALVYISGALLNNYSAIAVMSSMNLSLHHAIILAVMC
                     LISHNLIVETTVMKSIGSSGTKMVILRVGVAFLAGFILNLILPSSYSEVQLFRTTAVA
                     HSPFWPMIGAWAISTLKLIGRIILYLLILMLAETLLEQFNLMERLSRWLGWLMRIFGL
                     KPSMSFLWIVINIVGYAYGAGIIKAARDEGRMTAKEGDLFNHHAAISHSILEDTVLYG
                     AISLPVFWLILPRILLSIIVVWLERLRRELYRRSLKAGVA"
     gene            10667..11257
                     /locus_tag="PLQ30_03795"
     CDS             10667..11257
                     /locus_tag="PLQ30_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPW01598.1"
                     /translation="MKFAEPQTAETETKKPEEPLNERFLKTRTVLLVGEIDKDLSEKV
                     VRQLLLLESISEEPITLLIDSPGGDVYAGFSIFDVIRFIKAPVRIVGIGLVASAAALI
                     LIAVPKDRRFGLPNSSYLIHQPLSGMNGVATDIEIHAKELERTRLHINELLAEATGQA
                     LEKIIKDTDRDFWMNAQEAMQYGLIGKVIVSRQELD"
     gene            11367..12551
                     /locus_tag="PLQ30_03800"
     CDS             11367..12551
                     /locus_tag="PLQ30_03800"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPW01599.1"
                     /translation="MRPAFLFLSVLAMLAAGCQCSLPALRTDGEINIVSYNVHNLFDA
                     EESGKEYPEFRPSKGKWTKELYAKRLGNVVEAVRSLSNTKDGEGSPGSDPDILCFQEL
                     ENEKVLFDLAKHLGHGKLRYWAMSGPEESIIHTGILSRFPIVAVHSHSVMDAWGLGML
                     RDVLEVEFECGSMGRMVIFACHWKSKLEGEAETEPARRAAAALVANRIRLLREERPFV
                     PIIVCGDFNESPDEYLRTNRCYPTAIMYSGVGVPPMAIEGEPIWACSSLEEIGDESSE
                     LAESHSQVGIINGEGIDSYSIKHGKGVDFDEKIELYTPWAEYPDKFSLAYRDKREQYD
                     GFLVSQSLKDGIGLEYQGFYIADAPMLFTAEGVPREWKGTDGYSDHLPVELVLSVVDG
                     TQ"
     gene            12746..14077
                     /locus_tag="PLQ30_03805"
     CDS             12746..14077
                     /locus_tag="PLQ30_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HPW01600.1"
                     /translation="MVSGIVALILLLLAVIAIVVLTGKYRINAFLVLIGVSFVFGLLI
                     GLPALDVVVGIKNGFGGTLTSIGIVIVAGTILGTILEKTGAALSMTRAILNMVGKSHA
                     PLAMNIAGYIVSIPVFCDSGYVILNPLNKALAKESGISMTVMAVALSTGLYATHTMVP
                     PTPGPIAAAGTLGADLGKVILLGLIAAIPASLSGLLWATKFAKRYEIEPEIQGTYSEI
                     VKKFGKLPNTLLSFLPIVLPIVLILLKSVAEFPSKPFGNGDFRVFLSFIGDPVTALIL
                     GVLCSLLLVHKGELKKAISDWMGEGIKESAIILVITAAGGSLGAIIKASPITDFIKTN
                     MASMHLGILLPFIISAALKTAQGSSTVAIVTTAGIMAPLLQTLGLDPALTTIAIGAGS
                     MVVSHANDSYFWVVSQFSGMPVNTAYKAYSSATAVEGVVAFLMVLVLSLFV"
     gene            14098..14808
                     /locus_tag="PLQ30_03810"
     CDS             14098..14808
                     /locus_tag="PLQ30_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW01601.1"
                     /translation="MNKFYANICERFGLNAYVMGDYQKAESWFRKLEQSEPDSIRVLR
                     NLGIIFLALGRAEEAERYLLREEKLYGPSFYRNSALGDLAYATGKRKEAEKRYRLALK
                     DPECAKGGKAFHMRDFIERRLAMTEDDKRFCDAIKAMELFKKAEELRESSEHIKAIEY
                     FMKSFALDETNWPALNNAASIHLNMLKNPAEAEPLFRQAFELSRSPQVARNLDLCIQA
                     LNQNQKKEGQKKRWRQRT"
     gene            14790..16235
                     /locus_tag="PLQ30_03815"
     CDS             14790..16235
                     /locus_tag="PLQ30_03815"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HPW01602.1"
                     /translation="METTHVSESPGHSLRADAEEIFRAAVDRVMPGRLFSYCLSLEGS
                     VLRLLDGEEPQTYDLSKYDHVVIVAFGKASIPMTASLASILGQRVTEGLVVSKAPESG
                     QSSRLSEDIESLFASRSITSIESSHPVPDKRSVQAGSEMLNLALRVRKWEEAGERTLV
                     FVLVSGGGSALLAVPAPGISLEDKAEVTRLLLGCGANIHEINAVRKHLSSIKGGHLAK
                     AFFPSRIIALVLSDVMGDDLDTIASGPTVPDTSTWRDVKAIFERYALLDRLPKSIAQA
                     VEDGLSGIRSETPKPEDPIFDLCSTMLVGTNYHAIQEAEQRSKELGYNTLVLGSRFSG
                     EAREIGKVFSGIAQDIILHRTPLCAPACIIAGGETTVTLRGKGKGGRNQEMALSVLNE
                     FDHFSRSVRPGLERTLFLSAGTDGNDGPTDAAGAFADAGALENAHRLGLNPSEFLANN
                     DSYAFFEEVGGLFVTGPTGTNVCDIQLLIVK"
     gene            16256..17035
                     /locus_tag="PLQ30_03820"
     CDS             16256..17035
                     /locus_tag="PLQ30_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPW01603.1"
                     /translation="MKSDTQLKLAVLIDADNTQPTIIDGLLAEIAKYGVASVKRIYGD
                     WTNPTLRGWKERLLEYAIQPVQQFAYTTGKNSTDSAMIIDAMDLLYTENLDGFCLVSS
                     DSDFTRLAARLREDGKLVLGFGQRKTPKPFLAACDKFIYTEILQANVNETEEGRESSS
                     EKEGGKGSLPQADIKSDTKMKALLASAVDEAADEFGWAYLGEVGTYLANRLPEFDPRN
                     YGFRKLGELIKAVNLFEIDERTNPMVPGKQVYLRLKQRGKS"
     gene            17204..18019
                     /locus_tag="PLQ30_03825"
     CDS             17204..18019
                     /locus_tag="PLQ30_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPW01604.1"
                     /translation="MRALSFLATTFILAFILVVIVSCSGQKDNRLSSLPSVIRDQVEK
                     ALSVVQFSDTDRNRVRDAINNDPAGFAVLFSEVREIMKNDTDLLCRVDKQKALSAQYV
                     PIDLVDLDGPFPYIVSKKGMKLRKPAQEALVRMAEMAKKDGIALVVSSAYRSYDYQKI
                     VFEQNVNEMGRAEAERVSAVPGMSQHQLGTVVDFGSISDEFAGSDAERWLASNAGKFG
                     FSLSFPKDMENITGYKWESWHYRYISPPAVELQERFFLGIQQYLIEFLAALPN"
     gene            18226..19941
                     /gene="murJ"
                     /locus_tag="PLQ30_03830"
     CDS             18226..19941
                     /gene="murJ"
                     /locus_tag="PLQ30_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPW01605.1"
                     /translation="MTLNNQENSNNQENSNNQKNSSIQENSYDIVGFPEDTAAELASQ
                     ASAKMVRNAGKLSLLTFISRVLGLIREMTKARFLGTGIYSDAFTVSFIIPNFLRRLFA
                     EGSVTVAFIPTYKGYLHAQNDSETREFLSASLTILSICVTIVVAIGIALAPIIVKLFG
                     SDPMETAILTRIMFPFLAFVSFAALLQGILNAHEIFGPSGFAPILFNVCFIVVPWILG
                     DRLGNPARSMAVGVVLGGLAQALVQLPAVLRLSTHIGLMNPIRALRNSGMRKVFALIA
                     PTILGMAAYQVNDLVSTAYASRAGVGVASSLQYSLRLQELILGIFAVSAGTVLLPRLA
                     DTVRQGDWAEYSKILGNTMRSLLLLTVPVAVFSMAFTVRIVTLLFKTGDFSDVSVQLT
                     ASAFFWHQTGLVFIAMNRLIAPAFYARSNTKTPTFAGIASFAVNIILVLLLAFRFQGA
                     GIAFALSFSSAVNTVLLVWALIKDSIEGIRNELWLSLKYILKMLIFSAIAIVPAILVD
                     KAVFKAVSQYGSRFVYAGLPLVTSTLVFAAIGIALLVLTKDTVAASITTALSRRSRGK
                     NASQS"
BASE COUNT         5083 a         4437 c         5132 g         5302 t
ORIGIN      
        1 atctcagcct tctgtaatgc tggattctcg actttgcgat cgggcatgga gagctttcgg
       61 gataatgttc ttttgaatgt ggtgacgatg ctgctgatag tctctggagg aataggcttt
      121 atcgtgcttc aggatattat gcggtttgtc aagaaagaaa agcgtcgtct ttcctatcat
      181 tcctcagttg ttttgcgcac taccgcggtg ctaattgtgc ttggggctat tgttttctat
      241 gtgctcgagc agaatcacgc atataaggaa atgagccagg gttcgaaagt gatggcagcg
      301 ctttttcaat cggtaactcc aagaacctgc ggtttcgata ccgtgtcaca ggcgaatctc
      361 tcgagttcaa gccaacttgt tacaataatc ctcatgttca ttggcgcttc gcctggctct
      421 actggtgggg gcatcaaaac tgtaacgcta tatattcttc ttcttgtcgc attgaggatg
      481 agagaagggg gagatacgat tgtggaccga tataggacag tgactcacaa tgcggtgctc
      541 aaggcaatca cagcgcttgt gagaggcgca accattgtgt tggtcgccac cacaatcctc
      601 ctcgtctctg agagagcagc cgggcacacg gtaggacttg agactgctat tttcgaatgt
      661 gtttctgcct ttggaaccgt tggcttatcc ataggcataa ctccatacct tacggctatt
      721 tcgaagcttg tccttatagg cgcgatgttc atggggaggg ccggattgtt tattctcgca
      781 attccacctt cgagatattc accagagcct tttgcaaaat atcctaaggc agacctgctg
      841 ttataatttg ttcttatatg gaaggtataa tatgaaacaa tttgcgttta ttggtctggg
      901 accatttgct atgtcaatgt tggaacggat ttccgagatc agcggccaga ttattgccgc
      961 agatgagagc cctgagcgca tcgaacatgt taaggagatg gtctcggtgg cttttacaat
     1021 gaatctacta gatgaagaag cattcgaacg ggcatttcac gagccagtgg atgtggccat
     1081 tgtcgatata gagtcgaatg gtccggagct cttgctggtg actttcaggc tcaagaaatt
     1141 gggcgtaccc gaaatcatag taaaatcgaa ctcggaggaa tatgaagaaa ttctgcggct
     1201 ggttggtgca actcgcgtag taaactcgga cagagaggct gccctaagag taactccact
     1261 tgtgctttct tcttctctca caaactttat gcctataggg ggtgatcttg tacttgcaga
     1321 agttatagct cccgattttg ttgtgggtaa gagcgtcatc gagggtgatc tgcgcaatac
     1381 gcgtcatatc aatgttgtcg ccgtaaaata tgcttcggag atggattcct ccgggtcaca
     1441 aagcgcaatc tttcgtaatc tcgatacaaa ataccgcttt aaagatggcg atgtgctgct
     1501 tgtaacgggc aaagagaacg atgttttcga gttttcaggt attcaaaaga ctatcgaaca
     1561 tgaaaaagaa agagccaaga cgataagttt tctcaaaggc atgttcagcc gcaagaagaa
     1621 ggatgtccca aagaagtagc ctttggcttg tgcgagcctg cgcgctcgtt gattccattc
     1681 gctcgaacca ttacaataga aaagcggctc aggcatgata tacagcctgt gcgctccggc
     1741 ggcaaatagc cgttccggca aactgcgttg tggaaaggat ccgcgcgcgc aatggaggtc
     1801 ttttatggac atttttcaaa agtgctttga ttttgatctc gacaagcagg ccatggaaaa
     1861 agggttatat ccctatttcc ggccgcttga tgggctcgag ggaaccgagg ctgtctttaa
     1921 tggtcgcaag ctgatcatga tcggttctaa caattatctt ggactcacaa tgcatccaaa
     1981 agtcaaggaa gccgctagaa aaggtctgga agaatttggt ccttcttgta caggaagccg
     2041 gttcctaaat ggtacgctca aacttcacaa tgagctcgaa gagcgccttg ccacttttgt
     2101 tggaaaagaa gctgccctgg ttttttcgac cggcatgcag actaatcttg gagctatttc
     2161 atcgttggta gatcgcaacg atgttgtcat tattgataaa gatgaccatg cttctattgt
     2221 ggacggttgt aggctcagca tgggtgaaat gaagcgctat ttgcatgccg atctggacca
     2281 tctcgaacgc attctgcaaa aggttcccag cgaatcagga acaatggtcg ttgtcgatgg
     2341 tgttttctcc atgggaggag acattgtcga cttaccgggc ttggtaagac tctgccaaaa
     2401 atatggagcg cggctctatg tggacgatgc acattccatt ggcgttctgg gcgacggcca
     2461 tggtacggcc tggcattttg gtcttaccga taaagtcgat atcataatgg gcaccttttc
     2521 aaagagcttt gcatcgttag gtggttttgt ggcaggtgat agagatgtaa ttaattatat
     2581 aaaacataca gcccgctctt ttatgttttc tgcttctctg ccagcaccta ataccatggc
     2641 agcccttgct gctctagata tcatggagac agagcccgag catgtgaaac atctttggga
     2701 taatgccaat ttcatgatga aaggctttcg agagcttggt tttaatatcg gaaaaactca
     2761 gaccccaatt atccccgtaa ttataggtga ggatgaaaca tgcttccgtt tttggaagga
     2821 gctcttcgat gaggggctct ataccaatcc agtgataagc cccgccgtac cagaaggaat
     2881 ggctctattg cgcacttcct acatggcgac tcacactaga gagcaactgc agcgcgcgct
     2941 cgatattttc gagaaagcag gcaggaagtt tggtatcatc tagtcaataa tatccgaagt
     3001 tagcttgcgg cggtcaaaat ggcatttcag gcgcggatag caaaataggc ggcagcagat
     3061 ggcaaaatcg acagcatcgg ctggcttttg gactatctgc tcaggctatc tgcgcccttt
     3121 tttattttat aattcgtttg gcagagtctt atcggtactt ttctgtctcc tttgagctct
     3181 gcgattggcc agagcttgct agcgcggact agtttccctt ggttggaact atgagcccat
     3241 gttccataaa actgaaatag ccggattcag agaagatgag atgatccagc acgggtatac
     3301 ccaggatatc acccgattgc cgaagccttt tggttatgtc cacatcttcg tccgaaggct
     3361 ccagattccc gcttggatga ttgtgcgcaa ctacgaccgc gcaggctctg tcggttatag
     3421 gatcagcaaa gacctccctg ggatgcacaa ccgttttatt tatgagtcct ttggttatct
     3481 cgcgaatggc attaacctca tgaccgccat ttaacgatat gacgatgaaa tgctcttgtc
     3541 ttatatccgc gaaatggata aggagtggat aggcatcctt gggcattgct atgcgagtgc
     3601 cgcgagtcga gaaaatccgt ctcccaagct ccaacgcggc agctaccgcg caactctttg
     3661 cttctcccat tccagatatg agcataagtt gatcgaggtc gcatgaatcg ctgcttttgg
     3721 taatctggtt cagtacctct ttggcaagct tgcgaacggg tttgcctgga actcccgaac
     3781 ccagaattgc aacgatcagc tcgagattag atatcgagcc gctgccaagc ttcaacattc
     3841 tttcccttat atccagcgaa gaggagttag attcaaattt ttgtcccatt ttttcgtatg
     3901 cttgccattc cttcattgtg tctcctaatg tctatgacgc atcaagagtg attctcgatt
     3961 caatgttttt cggcttagat atcatcgaaa tagcatccaa atagcatcca aaatgctatt
     4021 ctatcactat attagctcca aaaggctcaa gaatgcccaa agtagcccta taaaagtaca
     4081 atgaactcat aagaaagccc taaacctaaa ggaaccgcaa ggtcacgcat gtagagatca
     4141 gtacaaataa gaatccgctg aaagtatcat caattggccg atcaggcatt tgcgtttcga
     4201 gatcggctgg aatataatca aaagtgatac ttggtttttg ggaaaattcc caatttcgag
     4261 aggaggagat tgtgaagagc ataaagggca ccaagacaga agcaaatctt cttactggat
     4321 tcgcgggaga atctcaggcg cgcaatcgct atacttattt cgcctcgaag gcaaaggaag
     4381 aaggttacat ccagatacag cttatcttcg aagaaacggc aaatcaagaa aaagagcatg
     4441 caaagcgatt attcaagttt ctagaaggtg gaaccgcaaa agtctccgcc gaatttccag
     4501 ccggcgtaat cggcacaacg cgagagaatc tcgatgaggc agcgggcgga gagaattacg
     4561 aatggcagga gatgtatcca acttttgcca aagtggcaag agatgagggt tttgaggcaa
     4621 ttgcgagcgt atttgattcc attgccgtgg ctgaaaaaca gcatgccagg agatatgaag
     4681 cgcttatgac gaacatcgat gaagggcgtg tcttcaaacg ggaggagcct gtgatttggc
     4741 gctgcataaa ctgcggttat gtctacgagg gaaccgaacc gcctaaagca tgtcctgcat
     4801 gtgctcatcc tcaagattat ttcgagctat tggccgaaaa ttggtaatta aggaggctat
     4861 ggtatatgac agagagaaag caggtctaca agtgcatgaa gtgcgggaat atcgtagaag
     4921 tgcttcatgc aggccagggg cagcttgtat gctgcggaga acccatgaga ctcatggtag
     4981 agaacacgag cgatggtgca aaagaaaagc atgtgcctgt acttgaaaaa actgccgatg
     5041 gttggtgggt aatggttggt tcgatcttac atccgatgga agaaaagcac tacatagaat
     5101 ggattgagct tatctctgat gagaattcct atcgggtcta ccttgcgccc gggcaagaac
     5161 ccaaagcctt tttccccgta aaggcggaaa aggtgcaagc aagagaatat tgcaatcttc
     5221 acggattgtg gaagacatga gagatcctaa ttcttaagga gcgcattatg aataagtatg
     5281 tatgtgatgt ctgtggttac atttatgatc ctgcagaagg agatcctgag gggaagatac
     5341 ctgctgggac tccgtttgaa cagctgccgg acgactgggt ctgccctatg tgcggtgcaa
     5401 gcaaggattc attttcgcca atggattgaa aaagagctct gcccgatatt tggcgggtat
     5461 tctttccaga atggcacaat aagaaagagg gagtctgcgc tccctcttat ttaattttat
     5521 atatcgcatt attctaaggc attattctct gagagcttgg agtgtctatg atttcccgcg
     5581 atgattttgt gttctgtgtg ggttatgatg gcgataccgc gattatagat ggcaaggcca
     5641 agaaggaatt ttgtgggctt tccaccatgg agcttgctga aaaaggtttg taccgtgcgg
     5701 cttttgcgtc cgcgctctat tctcaaaatc ccgaagagat gaaagtcttt atcgattttt
     5761 tcaacaagaa agcaggcaca gcctatacag aggccgggca gctttccagg ctttttggtg
     5821 tgtatctgga aacaatttca aaggtaaagg cattatagct cgtgcccctc tcgatgagtc
     5881 gaaaacagat catgggggtc attgggcgcg tctattgaac ctacgcttcg cctcatatac
     5941 gattatggca atatgaaaaa gccttcgacc atatatcgct gttcagcctg tggccatgaa
     6001 gaaccaaaat ggcttggacg ttgtcccgag tgcggccaat ggaacaccat ggttgaagcc
     6061 aaaaccatag ggcgctttga aaaggataag actgcatttt ccgtaccgct ggaatcggtg
     6121 gatccagctc tgggggcgcg cgtatcgtct ggaatttcgg aactggatcg agtgcttggt
     6181 ggtggcttta tgcgaggctc ggcagtgctc ttgggtggtg agcccgggat agggaaaagc
     6241 acccttcttc tgcaagcatg ctcgaagttg ggggcaaaag gaaaagcgct atatatttcc
     6301 ggagaagaat ctgcagccca gatacgcctt agagccgaac gcatcaaggc tctttcgaag
     6361 cagatagagg tattttgtgg caacgatatg catgcttgtc tctctgtgat ggattcagca
     6421 catccgcttc ttactgttgt ggattcgatc cagacagtgc attcagagga ggcgggggct
     6481 gtgccaggaa cgccgaacca aatcaaattt tgcacccagg aattcgtcga atgggcaaaa
     6541 agccacgatt ctattgtcgt ccttgtagcg catgtgacaa aggatggaat gatagccgga
     6601 cccaaggcag cagaacatct cgtggacgcg gtaataagct tcgagcaagc ggagaatgcc
     6661 ctacgagtgc tacgaaccag caagaatcgc tttgggtctt cggatgagct aggatttttt
     6721 ctgatgagtg aaggggggct ttcggagctg caggatccat ctggaatatt tatgatcagg
     6781 cgcgagggac agttgccttc gggcattgcc ctggcaattg tatatgaagg atcgcggatt
     6841 ctgttagcgg aagttcaggc tttaaccatc ccttcgaaat cgggcatcat gagagtgtac
     6901 tccgatagaa tcgatcctct gcgtgtctct cggattgcgg ctgtgctcga gaagcagaca
     6961 aggctagatt tttccagcca agaaatctat gtgaatgtcg cgggaggttt gcggctaaca
     7021 gagcctgccg tcgatttgcc actggcatgt gctctttatt ccgcacgatc gggccagcca
     7081 ttgccggagg gttccgctct tgcaggtgag ctttcgctcg caggggaaat caggccggtg
     7141 cgttctatgg atcgtagaac aagggcggct catcagcttg gctttgcgcg tattattgga
     7201 ccaaaagcct tacccgctgg cgcggaggtc ccggcaaaga gaggtgcagc aaaagccgag
     7261 gtcgttggag cagaagcggc caagtctgga acagtcaggg tggaagcggc cgctctttcg
     7321 ggacagacat tgccctggct gggggcttct tctcttcgag aatcgctgcg aattctatgg
     7381 ggtcagggga cctaaaatgc agtttatcaa taatgggcaa cgaattgcag gacttgtcgt
     7441 gccgctttta tcgcttaaga agaatgagaa tgctgcatgc ggagtgttct cagatctggt
     7501 gagtcttggg aaaatagcaa agaaatgggg actttctttg attcagcttc tgccgctcaa
     7561 cgacacgggc aatggaccat caccctatgc ggccataagt gcctttgcac tacacccaat
     7621 ctatatttct ctctccgata cgatcgatac tatggcctta ctcggtctgc cttttaagga
     7681 cgaagtagcg aaggaactgg tgtacgccga gaaggctcta gatcgtagat ttggaaacgg
     7741 cgggcgaatc caacgagttc aattcgaagc ggtgctacag cgcaaaatag atgcgcttcg
     7801 caaggcatgg gaagcaagcc ggcaaatctg tagtcctctt gcggaagcct ttgcaagccg
     7861 agagtcgtgg gcaaaaccct atgcctgttt tgtcgccttg agagaaaaat tcggtctagc
     7921 accctggtgg gagtggccag agtggcaaaa tgtcttgccg gccgatatag acaagctctg
     7981 gtctaaccaa gaattagctg aagaggagcg atttcggctt tggcttcaag tcctggctca
     8041 ggatcagcta aggcatgcag ctcaaacagc acatgagctt ggcatcgata tcatgggcga
     8101 tatacctatt ctccttgcca aggattcagc tgatgtatgg tttcagcgag agattttcat
     8161 cctcgataaa caagccggag cgcctccgga catgtattcg cccaggggac aaaactgggg
     8221 ctttccactg tatgattgga atgcgttgcg tgcgcggaat tatgatttct ggcgccaacg
     8281 cctgcaggct gctgaaaatt tctacagcgg gtatcgcatc gatcatgtgc tgggattttt
     8341 ccgtatctgg gctataagca tccatgagag cgatgcttat cttggcgcct ttgagccttc
     8401 tggctatttg gaacctgcag agctcaattc gatgggtttt tcagacgagc gccttacatg
     8461 gctatcaaag cctcatattc ccgaatggaa aatacaaaag gctgagaaaa gatgtctcga
     8521 tgcaggaatg cccctgcttg cagaaagcga ggcttcaggc atagagaata aacaaaaagg
     8581 gaagcgagaa gaaaatcgag aagataatcg ggaagatgag cgagccgata taagggcgct
     8641 atttggcaag cgcctcgaag aactcaggaa atcctgtttt gtgcgcataa agaatgagcc
     8701 actctttcta ttcaaacctg aaatccgagg caccgcagac ctaaatgcac tctttgggcc
     8761 tctgtggagc gaattccccg aggctgcacc aattatcgat gaatatgccg ggataatgcg
     8821 agattggtgg atagacagaa cactatatca atatgagccg gatcgctttg tctttcagtg
     8881 gagttataga gaaacgacaa gctggaaaag cctttcgctc caagaacaac aggccttaga
     8941 ggcgaaggca aaggcactca gtgaggaatc gttgaacata tgggagaaac gaggccgcaa
     9001 acttctttcg atgcttgtat cggctagcga aatgcaagcc tttgcggaag acctcggcgc
     9061 tgttccaccc tgtgtcccca aggtattgcg tgaactcaaa atcccagggc tgcgagtatt
     9121 gcgctgggag cgagaatggg acaagccaaa tcagccatat ataccattcg atagatatga
     9181 agcgctctct atctcttgta catcggtgca cgactccaca agcttgcgac aatggtggca
     9241 ggaagaagcc gatagatcgc agatatgggc aatatgtcag gctctagcgg agcacgacga
     9301 gcttttgaaa tccttgcttc aacgctgcgg agatacggct cctgaacagc tcggctctaa
     9361 agaagctgcc gttctggtgc gcgcactggc actatcaggc tccattgttg ccgcctatcc
     9421 aattcaagat attctcgcat gccacacgga gtggcgatta cccaatgctc aagatgagag
     9481 gataaatgtg cccggtacta ccctcccaac aaactggatg tacagaatgc ctatcgatct
     9541 acaggcgctt gccaaggatg aagactttgc aggctttatt tctaaggtta tggctcgagg
     9601 agggcaagcg tgagcaactc ggcacaaaga atgcagcaga tcgcgcacac tacgatcaag
     9661 agaacaatcg atacatcgct gtttttgata aaaataatgg ttccggttag cctattggtt
     9721 gcacttcttg gctggagtgg attattggaa aaaatctcag tagtcttgca tccgattatg
     9781 cgacttttgg gtttgcccgg agaggctgca cttgtctata tctccggagc ccttctcaac
     9841 aattattctg ctatagccgt catgagctcg atgaacctca gtctccacca tgctatcatc
     9901 ctggctgtca tgtgcctcat ttctcacaat ctgatcgtag agaccacggt tatgaaatca
     9961 attggatcct caggaaccaa gatggtgatc ttgcgtgtag gtgttgcatt tctggctggc
    10021 tttatcctta atctcatact tccttcgtcc tattcggaag tgcagctttt ccgtacgact
    10081 gccgtagccc atagtccctt ttggccaatg atcggcgctt gggctatatc cactttgaaa
    10141 ctcattggac gaatcattct atacctttta atattgatgc ttgcagagac gcttttggaa
    10201 caattcaatc taatggaaag gctctcccgt tggcttggat ggctaatgcg catatttgga
    10261 ctaaaacctt cgatgagttt tctatggata gtgatcaaca tcgtaggcta tgcctatgga
    10321 gcgggaatca tcaaggccgc ccgcgatgag gggcgcatga ccgcaaagga aggggatctc
    10381 ttcaatcatc atgcggcgat ttcgcactcg atcctagaag acacggtgct ctacggggct
    10441 atttccttgc cagttttttg gcttatcttg ccgcgcattt tgctttcgat aattgtcgta
    10501 tggctggagc gcttgcggcg agaactgtat cgtcgttctc tcaaggcagg agttgcatga
    10561 gtttttcaaa ttgtcagtat cgttgccgtt ttatctcgaa atcgttacta taaaatcgag
    10621 tatagataaa gagtctctta cattattgca aggagcctta caagaaatga aatttgctga
    10681 gccacagacc gcagaaacgg aaaccaagaa acccgaagag cctttgaatg agcgcttcct
    10741 taagacccga accgtgcttc tagttggtga gatcgacaaa gatctctcgg aaaaagtagt
    10801 gcgccagctc ctattgcttg aaagcatctc agaggagcca ataaccctgc ttatcgattc
    10861 tcctggtgga gatgtgtatg ctggattctc catttttgat gtgattagat tcataaaggc
    10921 accagtcagg atcgttggca tagggcttgt cgcttctgcg gcggcgctga ttctgatagc
    10981 ggttcctaag gatcgtcgct ttggtttgcc aaattcttca tatttgattc atcagccatt
    11041 atctggaatg aatggtgtag cgacggatat agagattcat gctaaggaac tcgaaaggac
    11101 acgtttgcat ataaatgagc tgcttgcgga ggcgacaggg caagcgctgg aaaagatcat
    11161 aaaagatacc gatagagatt tttggatgaa tgcccaagag gcaatgcaat atgggctcat
    11221 tggaaaagtg atagtttcca gacaagagct cgactgaaga gttggggcat gtcttatccg
    11281 ggaattattg ctgtttcttg attttggcgc gtgaattctg aagcgctgat aatgccggat
    11341 agcgtctttc ttattaagga ggaaagatgc gtccggcatt tctttttctt tctgtgctcg
    11401 caatgttggc ggcaggttgc cagtgttctc tgccagcctt gcggacagat ggtgagatca
    11461 atatcgtgtc atacaatgtg cacaaccttt tcgatgcaga agagagcgga aaggaatacc
    11521 ctgagtttag gccttccaaa ggaaagtgga ctaaagagct ctatgccaag aggctcggaa
    11581 atgtcgtaga ggccgttcgg tcgctttcca acactaaaga tggcgaaggt tctcctggtt
    11641 cggatcctga tattctatgt ttccaggaac tcgagaacga aaaggttctc tttgatctcg
    11701 caaagcacct tggtcatgga aagctccgct attgggcaat gagtgggcct gaagagagca
    11761 taatccatac cggcattttg tcccgtttcc ctatcgtggc cgttcattcg cattcggtta
    11821 tggacgcgtg gggtcttgga atgttgcggg atgtcctgga ggtggaattc gaatgcggta
    11881 gtatgggcag aatggtaatc ttcgcctgcc attggaagtc caagctggaa ggagaagcgg
    11941 agactgaacc agctcgacgg gctgcagcgg cattagtcgc gaatagaata cgattgcttc
    12001 gagaagagcg accttttgtt cccataatcg tctgtggcga tttcaatgag tcacccgatg
    12061 aatacctccg cacaaacaga tgctatccta cggcgatcat gtactcaggt gtgggagttc
    12121 cacccatggc tatcgaagga gagccaatct gggcttgcag tagtctagag gaaataggag
    12181 acgaaagctc agaactggcc gaaagccata gccaagttgg cataataaat ggcgaaggaa
    12241 tcgattctta ttccataaaa catggcaagg gtgtcgattt tgacgaaaaa atagagctct
    12301 acactccatg ggcggagtat ccggacaagt tcagcctcgc ctatagagat aagcgcgaac
    12361 aatacgatgg cttcttagtc agccaaagcc tcaaggacgg cattgggctt gaatatcagg
    12421 ggttctatat tgcagatgct cccatgctat ttactgccga gggtgttcct cgcgaatgga
    12481 agggaacaga tggatattca gatcatttgc ctgttgagct tgttctttcg gttgttgatg
    12541 gtacgcaata atgactttct ttatctgtgg ggaaaatcag gatacatgac aattatgtgg
    12601 aaaggcatta gcgctgtatc cctggagctt tcgagcctta gaatcgttta atctcggaat
    12661 ccggatcgcg ccctatggga tgtaatgaga cgtccttatc ggagctagaa gcatcataaa
    12721 gtaaactata tatttggagg tgtatatggt ttctggaatt gtcgccttaa ttctcttgtt
    12781 gttagcggta atagctattg tggtcttaac cgggaagtac agaataaacg cttttctggt
    12841 acttattggt gtgtcttttg tctttggact tctgattggg ctaccagccc tggatgttgt
    12901 tgtggggatc aagaatggat ttggcggcac tctaacgagt ataggcattg taatagttgc
    12961 aggcactatt ctgggcacaa tactggaaaa aaccggtgcg gcgctttcga tgaccagggc
    13021 cattttgaac atggtaggca aatcgcatgc tcctctggcc atgaatatag ctggttatat
    13081 tgtatcgatc ccagttttct gcgattccgg ctatgttatt ttgaatccac tcaacaaagc
    13141 acttgcaaag gaatccggga tttcgatgac agtgatggct gttgcgcttt ctacagggct
    13201 atacgcgacc cacacaatgg taccgcccac tccggggcct atcgctgctg caggaacatt
    13261 gggagcggat ctggggaaag tcattctctt gggccttatt gcagctattc cagcctctct
    13321 ttccggactt ctctgggcta ccaagttcgc taagagatat gaaatcgagc ccgaaatcca
    13381 aggtacttat tcggaaatag taaaaaaatt cggcaaatta ccaaatacac ttctttcctt
    13441 tttgccaata gtgcttccga ttgtgttgat tctattaaag agtgttgcgg aattcccttc
    13501 taagccattt ggtaatggcg atttccgcgt gtttttgtct tttattggcg atcctgtaac
    13561 cgcgcttatt ctcggtgtgc tgtgttcctt gcttctggtt cataaaggag agctaaagaa
    13621 ggctatctcg gactggatgg gggagggaat aaaggaatcc gctatcatcc tggtaatcac
    13681 cgcagcaggc ggctcactgg gtgctattat caaggcaagt ccgataacag attttataaa
    13741 gacaaacatg gcgagcatgc atctgggaat tctgttgccc tttataattt ccgctgccct
    13801 caaaaccgcc caaggttctt ctaccgtagc tattgttacc actgcaggca ttatggcgcc
    13861 tcttttgcag acgctcggcc tagatccagc ccttacgacc atagcgatcg gcgcgggctc
    13921 gatggttgtt tcgcacgcta atgattccta tttttgggtg gtctcccaat tctcgggcat
    13981 gcctgtaaat acagcctata aggcttacag ctctgcaact gccgtggaag gagtggtcgc
    14041 attcttgatg gtgctcgtat tgtccttgtt tgtctagtgg gataatctag gaagccgatg
    14101 aacaaattct atgcaaatat ctgcgaacgt tttggcctta atgcctatgt aatgggggat
    14161 taccaaaagg cagagagctg gttccggaaa ctcgagcaat cggaaccgga ttcaatccgc
    14221 gttttgagaa acctcggaat aattttcctt gcgctggggc gagcggaaga agcagaacgc
    14281 tatcttttga gagaagaaaa gctctatggc ccatctttct atcgcaattc agcgcttggc
    14341 gatcttgcat acgcaaccgg caagagaaaa gaggcagaaa agcgatatcg gcttgcgctc
    14401 aaagatccgg aatgtgctaa aggtggcaag gctttccata tgagagattt tatagagcgg
    14461 cgcctcgcga tgaccgaaga tgataagaga ttctgcgatg caataaaagc catggagctt
    14521 ttcaagaaag cagaggaatt gagagaatcg agcgaacata taaaagccat tgagtatttc
    14581 atgaaatcct ttgctctgga tgagacaaat tggcccgccc tcaacaatgc cgcttcgatt
    14641 catttgaaca tgctaaagaa tcctgcggag gcagaaccct tatttcggca ggctttcgag
    14701 ctctctcgca gccctcaggt agctagaaat ctagacctct gtatccaggc actcaaccag
    14761 aatcagaaaa aagaaggaca gaaaaaaaga tggagacaac gcacgtaagc gaaagtcctg
    14821 gacactcctt gcgcgccgat gcggaggaga tcttccgagc agcggtggat cgggttatgc
    14881 cgggacggct tttttcctat tgtctttcgc tagagggatc ggttctgcgc cttttggatg
    14941 gagaagagcc tcaaacctac gatttatcca aatatgatca tgttgtgata gttgcttttg
    15001 gaaaggcttc gattcctatg accgcaagcc tggcctcgat tctaggccaa cgagttacag
    15061 aagggcttgt ggtctctaag gccccggaaa gtggacaaag ttcgaggctc tctgaggata
    15121 tcgaaagtct ctttgcgtcc cgatcgatca cttcgatcga gtcaagtcac cccgttccgg
    15181 ataaaagatc ggtgcaagcc ggaagcgaaa tgctaaacct tgctttgcgg gtgcgaaaat
    15241 gggaagaagc aggcgaaagg acccttgtat ttgtactggt ctctggtgga gggagcgctc
    15301 tgttggcggt acctgcgccc ggaatttcgc tagaggataa agcggaagtg acaaggctcc
    15361 tattgggctg tggagctaat attcatgaga tcaatgcagt acgcaagcat ctatcttcca
    15421 taaaaggcgg ccatctggca aaggcttttt tcccttcacg gataattgca ctagtccttt
    15481 cggatgtgat gggcgatgat ctggatacca ttgcctctgg tcctacggtt ccagatacga
    15541 gtacatggag ggatgtgaaa gccatattcg aaagatatgc gctcttggac aggctgccaa
    15601 agtccatagc tcaagccgta gaagatggac tttcaggtat tcggtcagag actccgaagc
    15661 cagaagatcc aatcttcgat ttatgttcga caatgcttgt gggaaccaac taccatgcga
    15721 ttcaggaggc tgaacagaga agcaaagagc tgggatataa tactcttgtg cttggaagtc
    15781 gcttttctgg cgaagctcga gaaatcggca aggttttttc tggaatcgct caggatatta
    15841 tactgcatag gactccactt tgtgcgcccg catgtatcat tgccggagga gaaacgacgg
    15901 ttactctccg cggcaagggc aagggtggaa ggaatcaaga aatggccctc tctgtgttaa
    15961 atgagtttga tcatttttcg cgctctgttc gtccaggcct tgaaagaacg ctctttttat
    16021 cggctggcac cgatggcaac gatggcccta ccgatgctgc aggagccttt gccgacgctg
    16081 gagctctcga aaatgcacat cgccttgggc tgaatccttc ggagttcttg gccaataacg
    16141 attcctacgc cttttttgaa gaggttggcg gcctttttgt gaccggaccg accggcacga
    16201 atgtgtgtga cattcaacta cttatagtga aataaattgc ttttggagat tatttatgaa
    16261 gagtgatacg caactaaaat tagcggtgct cattgatgca gacaatactc agcccactat
    16321 tatcgatggc cttctcgcgg agattgccaa atatggtgtg gccagtgtaa agcgtatata
    16381 tggcgattgg acaaacccga ctcttagggg ctggaaagag agattgctag aatatgccat
    16441 tcagcccgtt cagcagtttg cctatacaac cggcaagaat tctacagaca gtgcgatgat
    16501 aatcgatgcg atggatctct tgtataccga aaacctcgat ggtttttgcc ttgtgtccag
    16561 cgattcggat tttacgaggc ttgctgcaag gctgcgtgag gacggtaagc tcgtactcgg
    16621 ttttggtcag cgcaagacgc ccaagccttt tctagcggcc tgcgacaaat ttatctatac
    16681 cgaaattctg caagccaatg tcaacgaaac agaggaaggg cgcgaaagct cgtcggaaaa
    16741 ggaaggcggc aaaggttctt tgccacaggc cgacataaag agcgatacaa agatgaaagc
    16801 cttgcttgca agcgctgtgg atgaagccgc ggacgagttc ggatgggctt atcttggcga
    16861 agtgggaacc tatctcgcga atcgccttcc ggagttcgat cctcgcaatt atggttttag
    16921 aaagttgggc gagctgatta aggctgtgaa tctattcgag attgatgagc gtactaaccc
    16981 catggtgcca ggcaagcagg tctatcttag attaaaacag cgaggaaaaa gctaagatct
    17041 aggaagctaa tggaatagag gctgccacaa agccaatgaa gcgaaggtga ctcagcccga
    17101 tcttgtactc tccttagaat ctcaagccag cgtccgatag tcttattcta atggcatcct
    17161 gaccagagtt taaaattatc tcgatccatg gtatgatttt gtcatgcgag ccctgtcttt
    17221 tctggcaact acttttatat tggcatttat attggttgta atagtatcgt gttcgggtca
    17281 gaaggataat cgtctttctt ccttgccttc ggttatccgt gatcaagtgg aaaaagccct
    17341 ttccgtggtg caattcagcg ataccgaccg gaatcgtgtg cgggatgcaa taaataatga
    17401 cccagctggc tttgcagtgc tttttagcga agtacgcgaa atcatgaaaa atgatacaga
    17461 ccttctgtgt cgggttgata agcagaaagc cttgtccgct cagtatgtgc ccatagatct
    17521 tgtagatcta gatgggccgt tcccatatat cgtttctaag aaaggcatga aattgcgcaa
    17581 gccagcgcaa gaagcgcttg tccgcatggc agaaatggca aaaaaggacg ggattgccct
    17641 cgttgtctcc tctgcctatc gctcctacga ctatcagaag atcgtatttg agcagaatgt
    17701 caatgaaatg gggagagcag aagctgagcg tgtatccgcg gtccctggga tgtctcagca
    17761 ccagcttggc accgtcgtgg attttggatc cataagtgat gaattcgcgg gcagcgatgc
    17821 cgagcgctgg cttgcatcga atgcaggaaa attcggattt tcgctttcct ttccaaagga
    17881 tatggagaat ataaccggct ataaatggga gagctggcac tatcgctata tttctccgcc
    17941 cgccgtagaa cttcaagagc gtttctttct tggcatccag cagtatctaa tcgaattcct
    18001 cgcagcccta ccgaattagc cgaaaatgat ctgtccgagg atatctgaca cggcatcttg
    18061 ttcgttggtc tttgtagata caaggcgtgc tgctgagcgc acttccctgg cggcgttcgc
    18121 tggagcgcag ccccaaccgg ctcgctatga atactgcgcg cgtcgcaatc aaaatccatt
    18181 acacaggact tcacctttgt gcgcgcttga agtaggctag cctaaatgac tttaaacaat
    18241 caagagaatt cgaacaatca agagaattcg aacaaccaaa agaattcatc catacaagag
    18301 aactcgtacg atatcgttgg attcccagag gatacagctg ccgagcttgc ttcgcaggct
    18361 tctgcaaaaa tggttagaaa tgctggtaaa ctttcgctcc ttacctttat ttcgcgtgtg
    18421 ctcggcctca tccgcgagat gaccaaggcg cgctttcttg gaaccgggat ctattcggat
    18481 gcttttacgg tctctttcat tattcccaac tttcttcgcc gcctatttgc cgaaggctcc
    18541 gtaactgtag ctttcatccc cacatacaag ggttatcttc atgcccagaa tgatagcgaa
    18601 actcgcgaat tcctttccgc aagcctcacg attttgagca tctgtgtgac gatcgtagtg
    18661 gcgataggaa ttgcccttgc acctataata gtcaagctgt ttggttcaga ccccatggaa
    18721 acagctatcc tcacgcgtat catgtttcca ttccttgcat ttgtttcttt tgccgctctt
    18781 ttgcaaggca ttctgaatgc tcacgaaata tttggaccat ccggatttgc gccaattcta
    18841 ttcaatgtct gttttattgt ggtgccgtgg attctcggag atcgtcttgg taatcccgct
    18901 agatccatgg ctgtcggtgt tgtattgggc ggccttgccc aagccttagt tcaactgcct
    18961 gccgtattga ggctgagcac tcatataggg ctcatgaatc caatccgggc tcttaggaat
    19021 tccgggatgc gcaaggtttt tgctcttatt gcgccaacaa ttctaggaat ggccgcttat
    19081 caggtaaacg atcttgtgag caccgcatat gcttcgcgtg ccggtgtagg agtagcatcg
    19141 agccttcagt attcactgcg ccttcaagag ttgattctgg gcatctttgc ggtctccgcg
    19201 ggaacggtgc ttttgcctcg cctggccgat actgtaagac agggagattg ggcagagtat
    19261 tcaaagatcc ttggcaatac catgcggtct ttgctcttgc tcaccgtccc cgtggcggtt
    19321 ttttccatgg catttacggt taggatcgta acgctgctct tcaagaccgg cgatttttcc
    19381 gatgtctcgg ttcagctcac ggcgagcgct tttttctggc atcaaactgg cctcgtcttc
    19441 attgcgatga ataggctaat cgccccagcg ttttatgctc gaagcaatac caagacgcca
    19501 acatttgccg gaatagcgag ttttgccgta aatatcatcc ttgttctatt gctggccttt
    19561 agattccaag gtgccggaat agcttttgcg ctctcctttt cgagcgccgt aaatacagta
    19621 ttgctggtgt gggcgcttat taaagatagc atcgagggta taaggaacga gttatggctt
    19681 tctctaaaat atatattaaa aatgctcata ttctcagcta tcgctattgt gccagcgata
    19741 cttgtggata aggctgtgtt caaagcggtc tcgcaatatg gctcgaggtt cgtatatgcg
    19801 ggtcttcctc ttgtaacctc gacactcgta tttgcagcta tcggcattgc tctactggtg
    19861 ttgacaaaag atacagttgc tgcttcaata acgactgcat tgtctcgcag gagccgcgga
    19921 aaaaacgcat cacaatcttg acacatcccc acat
//
LOCUS       DAOMZL010000028        19906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_2386_length_19906_cov_14.960002, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000028 DAOMZL010000000
VERSION     DAOMZL010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 19906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19906
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2386_length_19906_cov_14.960002"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PLQ30_03835"
     CDS             complement(<1..889)
                     /locus_tag="PLQ30_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HPW01606.1"
                     /translation="MNHISDIIEEARKIGRRKFSVACAQEATVLEAVTEAYHEGIGEP
                     ILVGNPKAIEEAAKEANEGKGVDISPFRIIEENNIEKAAAIAVGLIRSGEAEFLMKGI
                     IDSSLYLRAALNKETGLNAGKIACHVAVMEVPTYHKLFAITDIALNIAPDLPTFIDMI
                     NSSVKVMKIIGVNNPKVAILAAVEKVNPDKMPCTATAAILTQMNRRDQIKNCIVDGPL
                     ALDNAISCESVRIKKIKSEVAGDADILVTPEIESGNILYKCLLHLAGAKGAAVIMGAS
                     VPLVLTSRADDAETKLASIA"
     gene            complement(886..1959)
                     /gene="buk"
                     /locus_tag="PLQ30_03840"
     CDS             complement(886..1959)
                     /gene="buk"
                     /locus_tag="PLQ30_03840"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPW01607.1"
                     /translation="MQSVVLVINPGSTSTKFAVFHGDRCIFDQTLNHSAEELSHFATI
                     ASQYEFRESAIQEALKARNFDMSSLNAVVGRGGLLHPIPGGIYRINEGMKADLLAAKY
                     GEHASNLGALIADAFAKQFGIPAFIADPVVVDELEDVARVSGHKLFKRISIFHALNQK
                     AVARRFAKERGVCYEDLTLIVAHLGGGISIGLHRQGKVIDVNQALSGEGPFSPERSGT
                     LPAGDLARLCFSGQYTIQEVLKMITGKGGMVSFLGTNDMREVERMRDEGNKDADFYFQ
                     AFVYQVGKYIGSLAPVVEGKVDAIILTGGIAFWKELTERISQMVSFIAPVVVYPGEGE
                     LEALALAGQIALEGEADIKEYVP"
     gene            complement(2344..3264)
                     /locus_tag="PLQ30_03845"
     CDS             complement(2344..3264)
                     /locus_tag="PLQ30_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW01608.1"
                     /translation="MFSIFKQFLFLIRGVKSYALVGPSGTGKSFRAKLVAQKYGIELI
                     IDDGLLIRGDQILAGRSAKKDPTYLGAVKTALFDDREHREQVARALQREKFRKILVIG
                     TSERMVQKICERLQLPHPVKVIKIEEIATKTEIEKAVQSRKVEGKHVIPVPALEVKRS
                     YPSIFYDSVRVFLRRSFGVGATLPKLYEKSVVRPEYARRGRVAISEAALSQMVVHCVS
                     EFDPNVRIMRLAIRNDTQGYRITIMLEVPFGTKLPSKVRALQEYIVDSIEKFTGILVA
                     EVNIVIDRLTTRSQKGQKWSQAIDGRVSSV"
     gene            3434..5410
                     /locus_tag="PLQ30_03850"
     CDS             3434..5410
                     /locus_tag="PLQ30_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase"
                     /protein_id="HPW01609.1"
                     /translation="MSLITHPFLGLPIGDPGKIPLSREELPSFEDIRLATQELILSPS
                     GWRKIFAKPELAAGYASWDYEQLPDNSLSPLLAPTDMVIVAGMALVFSSFIKEHCKPH
                     KPSILLGIDTRPTSPAIADIFARVLIGEEYEVRYLFIVPIPEITAYAAHVTTLDVDNE
                     FHSDGFVYVSASHNPPGYNGIKFGLGGGVLTGEQIRPLISDLKLFLSDPESSEIALMA
                     LEKADSRALTKCYENVAHWKRYSQSAYMLFAHRVFTDEETAEAQERILLEIAEACEKR
                     PFGIIAELNGSARTQSIDLDWLSGIGVQTRALNIQPGVFAHRIVPENESLDDCRKALQ
                     LAHNENAAYALGYVPDCDGDRGNIVYYGNKLGEAIPLEAQQVFALACLSELAYMQWKG
                     EKNRIAIVVNDATSMRIEAIARVFGAKVFRTETGEANVVCRAEKLRAEGWTVRILGEG
                     SNGGNITHPSKVRDPLSTIGSIIRLLRLGDAEKKETCFNLWLEAIDSPERYQSGYNLE
                     DVIESLPQWITTSAFEPHAALKIHAVDKIELKKAYQRLFLEEWPKMLPELEQRFDIVS
                     WRAFASLGPDEFEVESDFGSSKNGGLRIVLYDKADEPRAFLWMRASGTEPVFRIEVDI
                     KNGTCSDEAWLRRWHAGLVTEADLLAAPRQNNVG"
     gene            5432..5836
                     /locus_tag="PLQ30_03855"
     CDS             5432..5836
                     /locus_tag="PLQ30_03855"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HPW01610.1"
                     /translation="MGIRGELFSTRFICEGRTYFFNVKENRNGDIFLSIVESKPTETE
                     TFDRRSIVIFQENMEGFMRALRTAAGYMEKASQRPKPDRAAIQSSRTRQEPRQTHQAL
                     HESSSRAPRRFVIKKKPHSAGDSRESKSGASD"
     gene            complement(5788..6450)
                     /locus_tag="PLQ30_03860"
     CDS             complement(5788..6450)
                     /locus_tag="PLQ30_03860"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPW01611.1"
                     /translation="MKKSMNFCTLDSVLRIPIELVCGVDEAGRGPLAGPVAAAAVILP
                     KGFPIAILDDSKRMSARRREEAYCIIVEQALDWAVGWATVEEIEKHNILMASLRAMER
                     AFGGLSHLPALVVVDGLFAPSLSMNGTNIEAATMAKADGLIPAVMAASILAKVARDRL
                     MDCLDCIMPEYGFRKNKGYPTKAHRAAIERFGPSRFHRKGFQLLIQSDAPLFDSLESP
                     AE"
     gene            complement(6468..7409)
                     /gene="trxB"
                     /locus_tag="PLQ30_03865"
     CDS             complement(6468..7409)
                     /gene="trxB"
                     /locus_tag="PLQ30_03865"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPW01612.1"
                     /translation="MHQIYDFAVIGAGAAGLSAAQYGARANLNTIVIEELAPGGQALF
                     IDKLENYPGILEPINGLELAERMQRQAEQFGSSFFEASALSVRKEKGHFIIETSAGPL
                     ESLTVLIATGARHRRLDIPGEQEFVGKGVSYCATCDGPFFKGKRILVVGGGDSACDEA
                     IYLSKLSDKIVMIHRRDRFRAQKALSSRVLSNPSIRVLFNTVAKEIKGDSKVRSVVLE
                     NTISHELTEEPFDGIFIFVGSTPQTAFLKDIGVQFDESGYILTNPNMQTSIAGLFAAG
                     DVRATPFRQIVTAAADGAIAARSASDYIDEQRGQTYR"
     gene            complement(7466..8926)
                     /locus_tag="PLQ30_03870"
     CDS             complement(7466..8926)
                     /locus_tag="PLQ30_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HPW01613.1"
                     /translation="MRESSARSIGVFLSEISKGEKLARSLRECGASVAILNGYSEDLS
                     RQFDCIVAEIGLLKEREIQAVLDSSTALVLLEKAAEELHRTEDAEYSLGQFSNSALLR
                     ILPRNASSSRIKCTIYQLFAKKDSLLSSKNTIAAAQSQLRTLLSSLPDIVYVLDEQGY
                     FLYLNESIGLLGYLPSELVGKHFGTIIHPEDRPNISRDLVVEKIRQDNIMPANPPKLF
                     DERRSGSRMTRELEVRLLHKDGHVIYAMVNAYGEKAFDVPLFSDVVGKKLQTIGVIHD
                     ISAMHLYQESLEESLAAKERLLREIHHRIKSNLQLVASLAHLKQIDAQSSAAIEVLRD
                     LETQIKSIALVHEALYQSERIDQLSARDFFGQFCHFAEEALERVGSTVQIRVSAEDYP
                     LDPNLLVPLSLALLYLLEGAYKVSYDRHEKVEVSLSFMRGQDGMQIIEMNGEGILENA
                     NSIVMQALLRQAHTSLIIPNTEKGRPSCHLIEEAKS"
     gene            9040..9924
                     /locus_tag="PLQ30_03875"
     CDS             9040..9924
                     /locus_tag="PLQ30_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HPW01614.1"
                     /translation="MLVSGMAFSFHTHTLFCDGKADATTMARTAFEHHYTHLGFSAHA
                     PISLETDCNLPWDEADSYVYAIRSLAQEYEPKGMKIFLGLETEYVPGIAKPDDPAYEP
                     MQLDYRIGSVHYVTTPDEYPFTVDESEDQFALHVQDWAPDGDYRKIWKRYWCFISEMI
                     EAGGFDIIGHFDLVKKNNIAGRWFDEENRAYTDAAFQAVDLAAEKDYIAEINTGGIAR
                     SKRGEPYPSVFILKRMQEKGIRITIGDDAHTPSHIGVYQPKAMDIAEMAGYKTLWYMD
                     APGVWKEVTLESVKAQLR"
     gene            complement(9926..10282)
                     /locus_tag="PLQ30_03880"
     CDS             complement(9926..10282)
                     /locus_tag="PLQ30_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPW01615.1"
                     /translation="MGNAFIDRMKNLLLAQRQEILNTIAESNKELRKALSETDPKDSA
                     DIASDDIDRIMIEALSSQDLKRYQAIESALLRINQGRYGLCAKCGKKIPEERLEAIPY
                     AVLCVECQKSDERKNR"
     gene            10547..10765
                     /gene="infA"
                     /locus_tag="PLQ30_03885"
     CDS             10547..10765
                     /gene="infA"
                     /locus_tag="PLQ30_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPW01616.1"
                     /translation="MAKEEAIEVEGIVKEALPNTMFRVELANGHIILAHLSGKMRKHY
                     IRIVPGDKVKVGLSPYDLNRGRIIYREK"
     gene            complement(10746..11213)
                     /locus_tag="PLQ30_03890"
     CDS             complement(10746..11213)
                     /locus_tag="PLQ30_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPW01617.1"
                     /translation="MPCKKPLSSSNIDQKISENSVSQTLERWLEVHEPVDKDKTNEDL
                     EADKRLDSISAYSRFKRILPQDELDLHGLYAEEARQRIQAFIADSASRGLEKVSIIHG
                     KGNHSHEEQVLINIVREELEKNPQTGEFDFADRKHGGRGATWVRIRPPISRDR"
     gene            11405..12685
                     /locus_tag="PLQ30_03895"
     CDS             11405..12685
                     /locus_tag="PLQ30_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPW01618.1"
                     /translation="MILNRRYSPTNFRFFTGLLRFTYGNWLKLRYRITAINTQLFKEL
                     KPPFLLVGNHTTILDPFIANAFVPFPVHWVASDGNMRNPIIRFMLIKLVGSIPKSKAI
                     PDIETVNWIVDLISHKHGVVGMYPEGQSTWTGTSFPVYSSSAKLLRLLRVPVVLVKTN
                     GGYLTKPRWSHVLRQGKVEISFSLLFSPEQLGAMRLSTVDRLLNEALSYDDSAWCKRK
                     GIEFKSTKGAESLELAIYLCPRCGERASMHSQGNLFTCKQCGFAVEYQHDGSFLLRSQ
                     GDREDYIPPFVEAPFLDSIVSWDSLQSRLLYQYLSGLKSAYLEAPFFLDEPIKLKRGK
                     RMDHMETLLNGRIELYRDRLEFHNTRNTKLDPIVFPIEQIDGEGVLKWNYFEFYHGMN
                     VYRVVFKDKRASGRKYADAISVLHQISKSHLPGR"
     gene            complement(12667..14082)
                     /locus_tag="PLQ30_03900"
     CDS             complement(12667..14082)
                     /locus_tag="PLQ30_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HPW01619.1"
                     /translation="MDFSWKIVIDAGLISFALVIATFLRSKVRFLQKFLVPNALTAGF
                     LLLPLYNFLMPAIGYGTNRLGDLVYHLLNLSFISMSLRSSPPKIKGSRSNGSVLGMSA
                     VILLGYASQGLLGLVLTLFFLPNIHPAFGLHLPLGFALGPGQAYAIGKGWESMGFEGG
                     ATVGLTFAAIGYLWACFGGMLLIHKGLRLGWMKPEQVSIMSDKALLTGIVPNGTEKPV
                     GERLTTDSEAIDSFSFHFAVVVFIYLLSYLALKGISFLLGFVGEAGTELAANLWGINF
                     IFSAIIAIIVRKIMDLLKVGYVLDDDTLSRISGTSVDYMVAGALGAISLVFVGKYWFP
                     ILLMSTLCGFMVYYTIPWVSSRIFRDFRFERMLMLYGVSTGTLSTGLALLRVMDPEFK
                     TKVTSDYMLSAGLTFVLAIPFILAINLPAKAYTSGSMVSYWIFMAIAAGYMLFVLVVY
                     LILARKRRFASPSANFYRPGK"
     gene            complement(14143..15048)
                     /gene="nudC"
                     /locus_tag="PLQ30_03905"
     CDS             complement(14143..15048)
                     /gene="nudC"
                     /locus_tag="PLQ30_03905"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HPW01620.1"
                     /translation="MTIFPESFVVFQGQDCLIPPQDAYNMRFLDEPSLAASIKPEAVC
                     WHFSQIPSDLLSPSSDHYAYSVFEFDDRKYGAIQIQDNDDILIKRPGLDEAIAFPVRE
                     LVVSYPSRFSRFMLIAKAHGQWASVSRFCGACGSLLVDAKENDEVRERLDDNAYAARF
                     CPRCKRIFFPRMSPAVIVLVYRENSILLGHNVRFPRNRHSLIAGFVEIGETLEETAIR
                     EIREEAGIEVKKLHYVLSQPWPFPDSLMIGFIAEWASGEARPDRNEIDHIAWYGADDL
                     PELPLRGSIARYLIDSFIAGGFSNG"
     gene            complement(15045..16115)
                     /locus_tag="PLQ30_03910"
     CDS             complement(15045..16115)
                     /locus_tag="PLQ30_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HPW01621.1"
                     /translation="MNRFILKAMDLDGYLSQSDLEYLSRMNELYEKALVSFNILSTTT
                     SEAQRHHEENFLIELYNQMGKLMQQICASEPRIQVYSFVSPQETHGEVSRLIAKLRDV
                     NTGHQEFVYYIQRAFELLFNLAFGGAGKTNKNYLIVRTPVTIPVQNYAVHKIPNVDSA
                     VHNTVMCVMLRGALLPSMILSKEIQEYSSTGYLTPFALFKIKRDESKSETTMEYVLDL
                     DHSYFELSELDGKHLLFADPMNATGGSLVTVVKYLLEQGVKPASIKFFNVISALKGTL
                     RVIRAIDNIMIYTLWMDPSLNDRAYIMPGLGDAGDRINGSDEDLHPRDMLRLVADYGT
                     NITGLYRSQLRIIEETVLRHRT"
     gene            complement(16171..16752)
                     /gene="pduL"
                     /locus_tag="PLQ30_03915"
     CDS             complement(16171..16752)
                     /gene="pduL"
                     /locus_tag="PLQ30_03915"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: protein motif:HMM:NF011652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HPW01622.1"
                     /translation="MPKMEIVINLSNHHIHVNHEDLEVLFGKGHELTKTKDLIQPGQF
                     ACAETVTIRGPKGQLEGVRILGPERKETQCEILASDVFKLGVQGCPVCESGQLEGSFP
                     FEVIGPAGSIKKERGLIIAKRHIHFDLEAAKHFGVSDKQIVKLKAGEERGAILLNVVC
                     RVHPTYALECHIDFDEGNALGITSGAIGEIVYC"
     gene            complement(16928..18403)
                     /locus_tag="PLQ30_03920"
     CDS             complement(16928..18403)
                     /locus_tag="PLQ30_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPW01623.1"
                     /translation="MILAVDIGTTRIKAALFDAEGRSLKLEIVDLPDESSNYYEEINA
                     RLWLKAFSEIISRLIATGEKNELKAIVICGNGPTILPIDERGEPLAPAITWLDRRAQQ
                     EAKEASIALNYSLDAAFNLPKILWMRQHLPNIYNRAHLFVSCPEFIAGRLSGEWTTCL
                     PNQGYTRIIWDTKALEMLRLDIEKFPRFVGVGEIVGYIHDHAAREFGLPNGLPVVMGG
                     PDFIASLLGTATVKPGRTCDKAGTSEGINLCSERDIGDEEALLVMPHIIPPYFNISGV
                     ISSSGIALSWFKSRFLPQEDFETIYARIASAEPGAEGLVFLPYLAGERSPHWDPNARA
                     VFLGLGLNHDLSTMARAVLEGTAFAIREVLDVMESRGATVHDMRATGTPSLSPIWNQI
                     KADITGKQLMVSSFPEPELAGCLAIGRFALGEESTLAEAAEKVFMPAEIFEPRKETES
                     MYCELYEVYLDSYRRLADLFPTISVLQRNNGVQRGRFGTGF"
     gene            complement(18562..19029)
                     /locus_tag="PLQ30_03925"
     CDS             complement(18562..19029)
                     /locus_tag="PLQ30_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPW01624.1"
                     /translation="MPDKLLKIGELASLFRVTARTIRYYEELGLIEASNRTEGFHRRY
                     PADAIIRLKRIEELKAMGLTLGQIREFFILYSQDPSGEKCRLLLLQNYEDQKKEEETR
                     ILEAQRRIAQIEANIIAIKEKKSFFSCPGEECKVCNFNTFCIDSEHNPDKEMR"
     gene            19164..>19906
                     /locus_tag="PLQ30_03930"
     CDS             19164..>19906
                     /locus_tag="PLQ30_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPW01625.1"
                     /translation="MRRFIIASLVAILTSTALWAQAPRIGVFVPGARDGSPIYDNMAK
                     GAERLVSETPGASLRVTEAGFNQAEWKEKLISFVASGKFDIVITSNPSMPELVNEASK
                     IFPKQRFICLDGYLAGNSSVYSALYNQLEQGYVTGYLSGLVSISGMKGTNPEKKIGMI
                     IGQNYPAMEKMIIPGFLKGLMAVDPGFQLDIRVLGNWYDAAKAAELSRSLYAQGADII
                     LPICGSASQGAVRVAQETGKYLVFFDDDE"
BASE COUNT         5476 a         4750 c         4354 g         5326 t
ORIGIN      
        1 gggcaatcga ggccagtttg gtctctgcgt catccgcacg gctcgtgaga actagaggca
       61 ccgaagcacc cattatcacg gctgcgcctt tagctcctgc gaggtgcaat aggcacttat
      121 agagtatatt cccagattcg atttctggcg tgacaagaat atctgcgtcg cctgcaactt
      181 ctgattttat tttcttaatg cgaacacttt cgcagcttat cgcattgtct aatgccagag
      241 gaccatcgac gatgcagttt ttgatctggt cgcgacggtt catctgcgtt aggattgcag
      301 ccgttgcagt gcaaggcatc ttgtcagggt tgactttctc gacagccgct agtattgcaa
      361 ccttaggatt atttactccg ataattttca ttaccttgac tgatgaattg atcatgtcga
      421 taaaagttgg aagatctggt gcaatattga gagctatatc ggtaatagca aatagtttgt
      481 gataggtcgg tacttccata acagccacat ggcatgcaat cttgccagcg ttgagacctg
      541 tctctttatt gagagcagcc ctgagataga ggctagaatc gatgataccc ttcattagga
      601 attctgcttc tccggaacga ataagaccga ctgctatggc cgctgctttc tctatgttgt
      661 tttcttcaat gattctaaag ggcgaaatat ccaccccttt cccttcattg gcttctttgg
      721 ctgcttcctc gatagctttc gggttaccta caagaatagg ctcacctatc ccttcgtggt
      781 atgcttccgt tacagcttcg aggacggttg cttcttgagc acaagcaacc gagaatttac
      841 gcctgccgat cttccttgcc tcttcgatta tatctgatat atggttcatg gcacatactc
      901 ctttatatca gcctctcctt cgagcgcgat ttggcctgct agtgccaaag cttcaagttc
      961 accttccccc ggatacacca ctacaggcgc aatgaacgaa accatctgac taattctctc
     1021 agtgagttct ttccagaatg cgataccccc ggtcaatatg attgcatcga cctttccttc
     1081 gactactggt gcgagagaac cgatatactt acctacttga tatacaaaag cttggaaata
     1141 gaagtccgca tccttgtttc cctcatcgcg catccgctcg acctcacgca tatcgttggt
     1201 gccaaggaaa ctcaccatgc cccctttccc ggttatcatt tttagtactt cttggatggt
     1261 gtattgccca gaaaaacaga gtcttgccag gtctcctgca ggcaaggttc cggagcgttc
     1321 tggcgaaaat ggcccttctc cgctcagagc ttgattgaca tcgataactt tgccttgtct
     1381 gtgcaagcca atagaaatac ctcccccaag atgagccaca attagcgtaa gatcttcgta
     1441 gcaaacaccg cgctcttttg caaatcgacg cgccactgcc ttttggttta gcgcatgaaa
     1501 aatgctaatt cgcttaaaga gcttgtgccc cgagactcgc gcaacatcct cgagctcatc
     1561 cacaaccaca ggatccgcaa taaaggcggg aattccgaac tgttttgcaa aagcatctgc
     1621 tatcagagca cctaggttcg atgcatgctc gccatatttt gcggcgagca aatcagcctt
     1681 cataccctcg ttgattctat atattccacc aggaattgga tgaagaagtc ctcctcgtcc
     1741 aaccaccgca tttagcgatg acatgtcgaa atttctagcc ttgagtgctt cttgaatcgc
     1801 actctctcta aattcatact gtgaagctat agtagcaaaa tgtgagagct cttctgccga
     1861 atggttcagc gtctgatcga aaatacatct gtcgccatga aaaacggcaa atttcgtgct
     1921 tgtagaccca ggatttatga cgagaacgac agattgcatg caatttctcc tgaattggtg
     1981 gcagttgact ttaagtatat catgtgtcta aggaaacgca agaggaaatc ttgcagaaaa
     2041 tgaaagaccg tttcaaatat caaaacgatc tttgtacgct cagagatgag ccagcctagt
     2101 tcagaaaaga ataaacatgc gaaaaataaa aaggccgccc tttcgattaa ggatggcctc
     2161 ttatcaagat aatatgtgcg gcgcgttcat ttgcgggtct tatggcgatt cagtcttctt
     2221 ttcttttttc tcttatgcgt cgctattttt tggagctttc gctttctacc acagggcacc
     2281 ttttttctcc ttggaaacga ttactgcaga atcattgtag gtactgccgc atatgtgcgc
     2341 atactacaca gaggaaacac gaccgtcaat agcctggctc catttctggc ctttctggga
     2401 gcgggtggta agcctatcta tgactatatt cacctcggca accagaatcc ccgtgaattt
     2461 ttcgatgcta tccacaatgt attcctgcaa tgcacgcacc ttcgagggga gctttgttcc
     2521 gaatggaact tcgagcatga ttgtaatccg gtagccctgt gtgtcgttac gaattgcaag
     2581 ccgcataata cgaacattcg gatcgaattc gctcacacag tgaactacca tctgggaaag
     2641 cgcagcttcg gaaatagcca cccttccacg tcttgcatat tcagggcgca caacagattt
     2701 ctcatacagt tttggaagag ttgcaccgac gccaaagctc cgtctcagaa aaaccctgac
     2761 gctatcatag aatatagaag gataactgcg ctttacttca agggcaggca ctgggataac
     2821 atgttttcct tcaaccttgc gcgactgaac ggctttttcg atttcggttt ttgtggctat
     2881 ttcttctatt tttattacct tgactggatg aggcaactgc aggcgttcac atatcttttg
     2941 caccatccgt tcgcttgttc ctattacgag aatttttcga aatttctctc gctgaagagc
     3001 tcttgcaact tgttctcgat gttccctgtc gtcgaagagc gcagtcttga cagcaccaag
     3061 gtaagtagga tctttttttg ccgaacgacc cgcaagaatc tgatcccctc ggattagaag
     3121 accatcgtct attatgagtt caatgccata tttttgcgct acaagttttg cccgaaaact
     3181 cttgcctgtt cccgaagggc caacaagcgc ataggacttt acaccgcgaa taagaaaaag
     3241 aaattgctta aagatactga acattccaat aaccaatata tcgctagaaa caattgaggt
     3301 aaataagcac ttttgctgag ttgccacaga cagaagcctg ctcttgtttt tgccctaatg
     3361 cagcgatttc gatcaatagc atcatcattc tcagaatcga gaaaagtagc tacaatattg
     3421 ctgggagcaa agcatgtcat tgatcactca cccttttttg ggtctgccta ttggggaccc
     3481 ggggaagata cctctatcta gagaagagct tccctccttt gaagatatca gactagccac
     3541 ccaagaacta atcctctcac cttcgggatg gcgcaagatc ttcgctaagc ccgagctcgc
     3601 tgcgggctat gcctcctggg attatgaaca attgccggac aactctcttt cacctcttct
     3661 agccccaaca gatatggtaa tagtggcagg aatggccttg gtcttttctt cgttcataaa
     3721 agaacattgt aaaccccaca aacccagcat tctgctcggt atcgacacaa ggcctacgag
     3781 ccccgctatt gcggatatct ttgctcgcgt acttattgga gaagagtatg aggttcgcta
     3841 tctgttcata gttccaattc cagaaatcac ggcatacgcg gctcatgtta cgactttaga
     3901 tgtagataat gaatttcact cggatggatt tgtctatgtc tccgctagcc acaatcctcc
     3961 ggggtataat ggtatcaaat ttggccttgg cggaggtgtg ctgactggcg aacagataag
     4021 accgcttatc tcagatctta agctttttct atctgatccc gaatcctcag agattgcact
     4081 tatggcactt gaaaaggctg actctcgggc cttgacaaaa tgctatgaaa atgttgccca
     4141 ttggaaacgt tattcgcaat cggcttacat gctctttgcc catcgagtct tcactgacga
     4201 agagacagcc gaagctcaag aaagaatact ccttgagatc gccgaggctt gcgaaaagcg
     4261 accttttggc ataattgcag agctcaatgg atcagcacgg acacagagca tcgatttgga
     4321 ttggctctcc ggaatcggag ttcagacaag ggctttgaac attcaacctg gcgtctttgc
     4381 ccatagaatc gtacccgaaa acgaatcgct cgatgattgc agaaaggctt tgcagttggc
     4441 tcacaatgag aatgcagcct atgctcttgg ctatgttccg gattgcgatg gagatcgagg
     4501 caatattgtg tattatggca ataaacttgg agaggcgatt cctctggaag cgcaacaggt
     4561 ctttgccttg gcctgcctat ccgagctagc gtacatgcaa tggaaaggcg agaaaaacag
     4621 aatcgcaatc gtagtcaacg atgcgacgag tatgcgcatt gaagccatag ccagagtatt
     4681 cggagcaaag gtcttcagga ccgagacagg cgaggccaat gttgtctgcc gcgcagaaaa
     4741 gctcagagca gaaggatgga cagtccgcat tcttggcgaa ggctctaatg gaggcaatat
     4801 aactcatcct tcaaaagtgc gtgatccttt atccactatt ggatctataa ttcgcttact
     4861 aaggcttggc gacgcagaga aaaaagaaac ctgtttcaat ctctggctag aggcaattga
     4921 tagtcccgag cgctaccagt ctggctataa tctcgaagat gtcatagaaa gcctgccaca
     4981 atggattaca acgagcgcct tcgagcctca tgctgcgcta aaaatacatg ccgtagataa
     5041 aatcgaactc aagaaggcct accaaagact tttcctggag gaatggccta aaatgctacc
     5101 cgaacttgag caaagattcg acattgtctc atggagagca tttgcatctc tcgggcctga
     5161 tgaatttgag gttgaatcag attttggctc ctcaaagaat ggaggacttc gaatcgtact
     5221 ctacgataag gcggacgaac ccagggcgtt cctctggatg cgagcttcgg gcacagaacc
     5281 tgtattccgc attgaggtag atatcaaaaa cgggacatgt tcagacgaag cttggctacg
     5341 cagatggcat gcaggattgg tcaccgaggc cgatctcctt gccgcgccca gacaaaataa
     5401 tgtaggatag gcgcatacaa aaggagttcc gatgggtatt cgcggcgaac tgttctctac
     5461 aaggttcatc tgtgaaggaa ggacttattt ttttaacgta aaagaaaata ggaatggtga
     5521 catttttctg tcaattgtgg agagcaagcc tactgagaca gaaaccttcg ataggcgctc
     5581 aatcgtaata ttccaggaaa acatggaagg cttcatgcga gcattgagaa ccgcggccgg
     5641 ctatatggaa aaagccagtc aaaggcccaa accggataga gcggctatac agtcttctcg
     5701 aaccaggcaa gagccccgac agactcatca ggcgttacat gagagttcat cgcgagctcc
     5761 tcgtcgattt gttattaaaa agaaacctca ttctgcggga gattccagag aatcgaaaag
     5821 cggcgcatcg gattgaatca ataattgaaa gccttttcga tgaaatcgcg atggaccaaa
     5881 gcgctctatg gcagctcgat gcgccttagt tggatatcct ttgttttttc tgaacccata
     5941 ctcaggcata atgcaatcga gacaatccat caggcgatct ctagccactt ttgcgagtat
     6001 ggaagcggcc attacagcag ggataaggcc atccgcttta gccatggtag ccgcttctat
     6061 atttgtccca ttcatgctca gagatggagc aaacaaacca tcgacaacaa cgagcgctgg
     6121 aagatgagat agccccccaa aagccctttc catggcacgc agactcgcca tcagaatatt
     6181 gtgcttctcg atttcttcta cggtagccca tcccactgcc cagtccagcg cttgttctac
     6241 gataatgcaa taagcctcct cacggcggcg agcactcatc ctcttagagt catcgagtat
     6301 tgcaatggga aagcctttag gcaaaataac agcagctgcg gcaacaggac ctgcaagtgg
     6361 acctcttcct gcttcatcta caccacatac gagttcaatc ggaattctga gcacagaatc
     6421 aagcgtacaa aaattcatcg attttttcat cgatacctct catcgattca tcgataagtc
     6481 tgccctcttt gctcgtcgat atagtctgag gcagatcggg ctgcgattgc accatccgcg
     6541 gccgcagtga caatttgtct gaatggagtt gctctaacat cgcctgcggc aaataaacct
     6601 gcaatgcttg tctgcatgtt aggattagtc aggatataac ccgattcgtc aaactgcaca
     6661 cctatatctt tgagaaaggc ggtttgaggt gtggaaccaa cgaatatgaa tattccatcg
     6721 aagggctctt cggtcaattc atgcgaaatg gtgttttcca ggacaacaga acggaccttc
     6781 gagtcgcctt tgatctcttt ggcaacggtg ttgaagagga ctctaattga agggttggag
     6841 agaactcggc tggagagagc cttttgtgct ctaaagcgat ctctacgatg tatcatgaca
     6901 attttgtcgg aaagttttga tagatagata gcctcatcac aggcagaatc gcccccaccc
     6961 accacaagaa tacgctttcc cttgaaaaat ggcccatcgc aagtagcaca ataagaaacg
     7021 cccttgccta cgaattcctg ctctccaggg atatcaagcc gcctatgtct tgcgcctgta
     7081 gctatgagaa ccgtgagact ctccagaggt ccagcactcg tctcgattat gaagtggcct
     7141 ttctccttgc gcactgataa tgcgctcgct tcgaaaaaag agctgccgaa ttgctcagcc
     7201 tgcctttgca tacgctcggc aagttcaagg ccattgatgg gctctagaat accaggatag
     7261 ttttccagtt tatctataaa aagagcctga cctccagggg ctagttcttc gattacaatt
     7321 gtatttaggt tggcacgggc tccatactga gcggcggaga gccctgctgc gcctgctcca
     7381 ataactgcaa aatcataaat ctgatgcatc aacttctcct ccatgccttt aatataatga
     7441 tttttatata ttacctgata cacatttaag actttgcttc ctcaattaga tggcaagaag
     7501 gcctaccctt ctctgtattg ggtattatca agcttgtatg ggcttgtctc aacagagctt
     7561 gcatgacaat ggaatttgcg ttctctagaa tcccttctcc attcatctca atgatctgca
     7621 tgccatcttg tccgcgcata aaagaaagcg aaacctccac cttttcgtgt ctatcatagc
     7681 taaccttata agcgccttcc aggagataaa gcaaggcaag cgaaagcgga acaaggagat
     7741 tcggatccag aggataatct tcggcggaca ctctgatttg taccgtggac cctaccctct
     7801 ccagagcttc ttctgcaaaa tgacagaatt gtccaaagaa gtccctcgca gacaattgat
     7861 cgattctctc cgattgataa agagcttcat ggacaagcgc aatgctcttt atctgcgttt
     7921 ctagatctct caaaacctcg atagccgccg aagattgagc atcgatctgc tttaagtgag
     7981 ccaagctagc caccaactgt agatttgatt ttattctatg atgaatttcg cgaagaagcc
     8041 tctcttttgc agccagagat tcctccaggc tttcttgata gagatgcatt gcgctgatgt
     8101 cgtgaattac ccctatggtc tgaagctttt tgccaaccac atccgagaaa agcggaacat
     8161 cgaaggcctt ttctccataa gcattgacca ttgcatagat tacatgtcca tctttatgca
     8221 gaagtcgtac ttccagttct cttgtcatgc gcgaacccga tctcctctca tcgaagagtt
     8281 ttggaggatt tgcgggcatt atattgtctt gccttatttt ctcaacaaca agatctcgcg
     8341 agatatttgg tctgtcttcc ggatgaataa ttgtaccgaa atgctttcca accaactccg
     8401 aaggcagata gcccaacaga ccaatcgatt catttagata aagaaaatag ccctgttcat
     8461 ccagtacata tacaatatcc ggaagagagg atagcagtgt ccgcaactgg ctttgcgcgg
     8521 ccgctatagt gtttttagac gagagtaaac tatccttctt tgcaaatagc tgatagatag
     8581 tacatttgat tctcgagctg gatgcatttc tgggcaaaat ccttagaagt gcagagttcg
     8641 agaattgccc taaagaatat tcggcatctt cagttctatg caactcttcc gcagcttttt
     8701 cgagtagcac cagagcagtc gagctgtcta gaacagcctg gatttctctt tctttgagca
     8761 gtccaatctc cgcgacgata caatcgaatt gtcgtgaaag atcttccgaa tatccattca
     8821 ggatcgcgac agaagcgcca cattctctga gcgacctagc gagtttttct cctttgctaa
     8881 tttccgagag aaagactcca atagatctcg cagaagattc tcgcattcta tcatagtaag
     8941 aaagggcttt tacaacgtca atagacgaaa cttcagcatg attgcattta catttaattt
     9001 tcgtacaccc acatagtgca tagaagaaaa ggtagaaaaa tgctagtatc cggtatggcc
     9061 ttcagttttc atactcatac actgttttgc gatggaaagg cagatgcaac cacaatggct
     9121 cgcaccgcat tcgaacatca ttacacccat ctcggatttt ccgcccacgc accgatctcc
     9181 cttgagacag actgtaattt gccctgggat gaggccgatt cctatgtcta cgctatccgt
     9241 tctttagctc aggaatatga gccaaagggt atgaagatct tcttgggtct ggagaccgaa
     9301 tacgtgccgg gaatagcaaa gcccgatgac ccggcttacg agcccatgca actagattac
     9361 cgcataggtt cagttcacta tgttactacc ccagacgaat atccatttac tgttgatgaa
     9421 tcggaagatc agttcgctct acatgtccag gattgggcgc cagatggaga ttatcgaaaa
     9481 atctggaaac gatattggtg ctttatttcc gaaatgatag aagccggtgg attcgatata
     9541 atagggcatt ttgacttagt aaagaaaaac aatatagctg gacgctggtt tgatgaagag
     9601 aatcgagctt atacagatgc cgcattccaa gctgtagatt tagccgccga aaaagactat
     9661 atcgcagaaa taaataccgg aggtattgcg cggagcaagc gtggcgagcc atatccatca
     9721 gtcttcattc taaaaagaat gcaggagaaa ggcataagaa ttaccattgg agatgatgcc
     9781 catacacctt cccatattgg ggtctatcag ccaaaagcca tggatatcgc cgagatggcc
     9841 ggctataaga cgctgtggta tatggatgct ccaggtgtct ggaaagaagt caccttagag
     9901 tcagtcaaag cccagcttag atagatcaac ggttctttcg ctcgtcgctt ttttgacact
     9961 cgacgcaaag caccgcataa ggaatagcct ctagacgctc ctccggaatc ttcttgccac
    10021 actttgcgca aagaccatag cgaccctgat tgattcgaag gagtgcagat tctatcgctt
    10081 ggtagcgctt gagatcttga gaactcaagg cttcgatcat tatcctatcg atgtcgtcag
    10141 atgcgatatc cgccgaatcc ttgggatcgg tctcagaaag ggcttttctg agctctttat
    10201 tcgactcagc aatggtattc aaaatttctt gtctctgagc cagcaagagg ttcttcattc
    10261 tatcgatgaa ggcatttccc atctctctcc ccctcatcag ttgctggtaa aaaaccagta
    10321 tgcagtggat gggcacaatg catcactttg gcgaaaatgt caatacatgt ggagagatgt
    10381 cctcatgttg acattctatg cattgattca tactatttca ttcgcattgt ggccgcaagg
    10441 atttttcttt tgtggctttt gtgcataaca tggttttgca aaggacggat gtagcttcca
    10501 tcaatgatgg atagacgtcc taagaattta ccatcaagga gtatatgtgg ctaaagaaga
    10561 agctattgag gtagaaggca ttgtaaaaga agctcttcca aacacgatgt ttcgcgtaga
    10621 attggcgaat ggacacatta ttcttgcgca tctctccggg aaaatgcgca agcactatat
    10681 caggattgtg ccaggagata aagtaaaagt tggcctctca ccatacgatt taaatcgagg
    10741 acgaattatc tatcgcgaga aataggaggt cgaatgcgga cccaggtagc acccctgcca
    10801 ccatgttttc tgtcagcaaa atcaaattct cctgtctgcg gattcttctc cagctcttcc
    10861 cggactatat ttattagaac ctgctcttca tgcgaatgat tcccctttcc atgtataatg
    10921 ctcacttttt ccaggcctct tgaggcgcta tcggctataa aggcttgaat cctctggcga
    10981 gcttcttccg catataatcc gtgaagatct aattcatctt gaggaagtat acgcttaaat
    11041 cttgagtagg cggaaatcga atccagtctt ttgtccgctt ctaaatcctc attagtctta
    11101 tctttgtcga cgggctcatg aacttcgagc catcgctcta aggtctgaga tacgctattc
    11161 tcgcttatct tttggtcgat attggacgat gacaggggct ttttacaagg catagacgtc
    11221 tcctcgtaat ctattgtttc atttaggttc ctcttcgaat gtatacatag cacattatta
    11281 tatttatttc attgggctga tatgaggatt tccagtgaat ccaaaaagcg agcaacgcca
    11341 ttcttatgac ccgtcttgct atattcaaat tggaacatta gaatcatgaa agggattagc
    11401 attcatgatt ctcaaccgac gatactctcc gacaaatttt agatttttta cagggctttt
    11461 gagatttacc tatggaaatt ggctcaaatt gagatatcgg atcaccgcaa taaataccca
    11521 gctcttcaag gaactcaagc cgccattcct tctcgtaggc aatcatacaa caattcttga
    11581 tcccttcatt gccaacgcat tcgtgccttt tcccgtacac tgggttgcta gcgatggcaa
    11641 tatgagaaat ccgatcattc gttttatgct gatcaaacta gtaggaagta ttccgaaatc
    11701 aaaagccata ccagatatcg agacggtgaa ttggatcgtt gatttgataa gtcataagca
    11761 tggtgtagtg ggaatgtacc ccgaaggtca gagtacttgg acaggaactt cttttcctgt
    11821 atatagttcc agtgctaagt tgctgcgatt attgcgagtc ccagtagtat tggtaaagac
    11881 aaatggcgga tatcttacaa aaccccggtg gtctcatgtt ctacgacaag gaaaagtcga
    11941 gatctcgttt tcgcttctgt ttagcccgga acagctagga gcaatgagac tctccactgt
    12001 agatcgatta ctcaacgaag ctctttccta tgatgattca gcctggtgta agcgcaaagg
    12061 cattgaattc aaaagcacaa aaggcgccga gagccttgag ttggcaatct atctatgccc
    12121 tcgttgtgga gagagagctt caatgcatag tcaaggcaat ctgttcacat gcaaacaatg
    12181 tggctttgcc gtggaatacc aacacgatgg ttcattcttg ctacgttcgc aaggggatcg
    12241 cgaagattat ataccaccgt tcgttgaagc tccttttctc gactcaatcg taagttggga
    12301 tagtctacag agtaggcttc tatatcaata tctctctggc ttgaaaagtg catatcttga
    12361 agctccattc tttcttgacg aaccaatcaa actaaagcgc ggcaaacgca tggatcatat
    12421 ggagaccttg ctcaatggcc gtatcgaact ctatagagac cgactcgaat tccataacac
    12481 aagaaatacc aaactcgatc caattgtttt tcctatagag caaatcgatg gagaaggcgt
    12541 gctcaagtgg aattatttcg agttctatca cggcatgaat gtctatcgag tcgtcttcaa
    12601 agacaaaaga gcatcaggca gaaaatatgc cgacgccatc agcgtactgc atcaaatttc
    12661 aaaatctcat ttgcccgggc gataaaaatt agcactgggc gaggcaaatc gcctttttct
    12721 cgctagaatc aaatacacta ccaacacaaa aagcatatag cctgcggcta tagccatgaa
    12781 aatccaatat gaaaccatag acccgctcgt ataagccttt gccggcagat tgatcgcaag
    12841 aatgaatggg atagccagaa caaaggtcaa gcctgcagag agcatatagt ctgaggttac
    12901 ctttgtctta aattcggggt ccataacccg taaaagagcc aagccagtag agagtgtgcc
    12961 agtagaaacc ccatagagca taagcatgcg ctcaaatcta aaatctcgaa atatccgcga
    13021 actcacccat ggaatcgtgt agtacaccat gaatccgcat aatgtggaca taagcagaat
    13081 cggaaaccaa tatttaccga caaatacgag tgaaatagcc ccaagcgcac cagcaaccat
    13141 ataatccacg gaagtacccg aaatcctgga tagggtatcg tcgtcgagca catatcccac
    13201 tttgagcagg tccataattt tcctgacaat tatggctata atagcagaaa agataaaatt
    13261 gataccccaa agattggctg caagctccgt accagcttcc ccgacgaatc caagcaggaa
    13321 agatattcct tttaaggcaa gatagctgag caaatagata aacaccacaa cggcaaaatg
    13381 aaaactaaag gaatcgatgg cctcagaatc cgtggtaagc cgctctccta ctggtttttc
    13441 ggtaccattt gggactatac cagtcagtaa agccttatca gacatgatgc ttacctgctc
    13501 tggcttcatc cagcctaagc gcaagccctt gtggatcagg agcattcctc cgaaacatgc
    13561 ccagaggtaa ccaattgccg caaaggtaag cccaacggtt gctcctccct caaaacccat
    13621 tgactcccaa cctttcccta tggcataagc ctgcccaggt ccaagggcaa aacccagtgg
    13681 aagatgcaat ccaaatgcag ggtgaatatt aggcaaaaag aatagagtta gaacgaggcc
    13741 aagaagtcct tgtgaagcat atccaagaag aatgaccgct gacataccca gcacgcttcc
    13801 attggaacga gaacctttga tcttgggtgg cgaagaacgg agagacatgc ttataaaaga
    13861 gaggttgagc aaatgataga caagatctcc aagtctattc gtcccataac ctatggctgg
    13921 cataaggaag ttatacaagg gcaaaagcag aaaaccggct gtcagagcat ttggcacaag
    13981 aaatttctgc agaaatcgaa ccttagacct caaaaaagtg gcaatcacca gggcaaagga
    14041 aataagccct gcatcgataa cgattttcca gctgaaatcc atgatttgct cctatctatc
    14101 cagtatagaa tcaattacat gcaaaattag atacaacaga gttcagccat tagaaaaacc
    14161 tccggctatg aagctgtcaa tcaaataacg agcgatactt cctctcaatg gaagctctgg
    14221 cagatcatct gctccatacc aggcgatatg atcgatttca ttcctgtcag gtcgtgcttc
    14281 gccagatgcc cattctgcaa tgaatccaat cattagagaa tcggggaatg gccatggttg
    14341 agaaagcaca taatgcagct ttttcacttc tattcctgct tcttcgcgta tttctcgaat
    14401 ggctgtttct tcaagcgtct caccaatctc cacgaacccc gcaattaggc tatgcctatt
    14461 gcgaggaaag cgcacattat gccccaacag aattgaattc tctctgtaca caagcactat
    14521 gaccgcaggc gacattcgag gaaagaaaat cctcttgcag cgcgggcaga atcttgcggc
    14581 atatgcatta tcatcgagcc gctctctaac ctcgtcgttt tcttttgcat cgacaagaag
    14641 actcccgcag gctccgcaga atcgggagac cgatgcccat tgaccatgtg ctttagcaat
    14701 gagcataaaa cgactgaacc ttgaaggata ggaaacaaca agctcacgca caggaaatgc
    14761 aatggcttcg tctagacctg gtcgcttgat caaaatatca tcattatcct ggatctgaat
    14821 tgccccatat ttcctgtcat caaattcaaa caccgaatag gcatagtgat ccgatgaagg
    14881 agataataga tcagatggta tctgagaaaa gtgccagcag acggcttcgg gttttatcga
    14941 tgcagcgagc gaaggctcat ccagaaaacg catattatag gcatcttgcg gaggtataag
    15001 gcagtcttgt ccttgaaaga cgacaaaact ctccgggaat atggtcatgt tctgtgcctc
    15061 agcaccgtct cttcgattat tcgaagctgg gaacggtaca atcctgtgat gttggttcca
    15121 taatctgcaa cgagtctcag catatctcgc ggatgaagat cttcatcgct gccgttgata
    15181 cggtccccgg catcgcccag ccccggcatg atataggcac gatcgttgag gcttggatcc
    15241 atccatagag tgtatatcat aatgttatcg atggctcgaa ttacgcggag agtccctttc
    15301 agcgcgctta taacattgaa aaacttgata gaagcaggtt ttactccctg ttcgagaaga
    15361 tatttcacaa ctgtgacaag agaaccgcct gttgcattca tgggatccgc aaagagcaga
    15421 tgcttaccat ccagctcaga aagttcaaaa tatgaatggt caaggtccag cacatattcc
    15481 atagtggttt cgctcttgct ctcatctcgc tttatcttga aaagcgcaaa aggagtaagg
    15541 taaccagttg aagaatactc ctgaatttcc ttggagagta tcatcgatgg aagtagagca
    15601 cccctcaaca tgacgcacat gaccgtattg tgtacggcgc tatcaacatt gggtatttta
    15661 tggacggcat aattctgtac aggaatagta acgggcgtgc gcacgataag gtagttttta
    15721 ttcgtcttgc ctgctccgcc aaaggccagg ttgaacagaa gctcaaaggc tcgctggata
    15781 tagtagacga actcctgatg tccggtattc acatctcgca gcttggcaat caagcgcgat
    15841 acttccccat gtgtttcctg tggcgacacg aatgaataga cttgaatccg tggctccgat
    15901 gcacagatct gttgcatcaa tttgcccatt tgattgtaga gctctatcaa gaaattctct
    15961 tcatgatgtc tctgcgcttc cgaggtagtg gtggatagga tattgaaaga aaccagcgcc
    16021 ttctcataga gctcattcat ccgcgaaagg tattcaagat cgctctgcga aaggtaacca
    16081 tcgagatcca tagcttttag aataaatcta ttcacacggt actccaatct taaggctcat
    16141 gctcccatcg cgcttgattc gttcattagt tcaacaatag acgatttcgc ctatggctcc
    16201 gctcgtaatg ccaagagcat taccttcgtc gaaatcgata tggcattcta gagcataagt
    16261 aggatggacc ctgcatacga catttagaag aatagctccg cgttcctctc ctgctttaag
    16321 cttgacgatc tgcttgtcac ttactccaaa atgcttggcc gcttccaaat cgaaatggat
    16381 atgccgcttt gcaatgatta gacctctttc cttttttatc gagccagctg gaccaattac
    16441 ctcaaagggg aaactgcctt cgagctgacc tgattcacat acagggcagc cttgcacgcc
    16501 tagtttaaag acatcgctcg ccaggatttc gcactgagtt tctttacgct cggggccgag
    16561 aattcgaacc ccttcgagct gtccctttgg ccctcttatc gtgacagtct ccgcacaagc
    16621 aaactgacct ggctgaatga ggtccttggt cttggtaagc tcatgacctt ttccaaaaag
    16681 cacctcgaga tcttcgtggt ttacatggat atggtgattc gatagattga tgactatctc
    16741 catctttggc atttaatcct cctggcaaaa agcgatatac tgaatgtggc catgatattg
    16801 tcttctcaaa agcacagtaa actatcctat cccagaagag attttgtcgc aagaatagga
    16861 atattatttg cagcaaagga acagggacga taatttttta tggccgattt gtctcgaaaa
    16921 gcctttctca aaaaccagta ccaaaacgcc ctcgttgaac accattattc ctctgaagca
    16981 ccgaaatggt aggaaagagg tctgcaagtc gccgataact atccagatag acttcatata
    17041 attcgcagta catggattct gtttcttttc gaggttcaaa tatttcggct ggcataaaaa
    17101 ccttttctgc ggcttcagcg agggtagact cttcacccaa agcgaatcga ccaatagcaa
    17161 ggcagcccgc taactccggt tccgggaagg aggataccat aagctgtttt cctgtaatat
    17221 ctgccttgat ctgattccat atgggagaaa gcgaaggtgt gcctgtagct cgcatatcat
    17281 gcacggtggc tcctctgctt tccattacat ctagcacttc tcggatagca aaagccgtgc
    17341 cttcgagcac cgctctcgcc atcgtgctga gatcgtgatt aagccccagc cccaggaata
    17401 cggctcgcgc attggggtcc caatgaggcg atcgttcgcc tgcaagataa ggaagaaaca
    17461 caagcccttc ggctccgggt tctgctgagg caatccgcgc atagatcgtc tcaaaatcct
    17521 cttgaggcaa aaacctgctt ttaaaccacg aaagagctat tccgcttgac gatattactc
    17581 ccgaaatatt gaaatatgga ggaattatat gaggcataac aagaagcgct tcttcatctc
    17641 cgatatctct ttccgaacaa agattgatgc cttcggaagt tccagctttg tcgcaggtcc
    17701 ttcccggctt aactgtcgct gtacccagta gcgaggctat aaaatcaggg cctcccataa
    17761 cgactggcaa gccattagga agaccaaatt cccttgcagc atgatcatga atatatccta
    17821 ctatctcacc aacacctacg aagcgaggaa atttctcgat atccagcctg agcatttcaa
    17881 gagcttttgt atcccagata atccgcgtat agccctgatt gggaagacag gttgtccatt
    17941 caccactgag acgccccgcg atgaattccg ggcaggatac aaaaagatgt gctctattgt
    18001 atatattcgg caaatgctgc ctcatccata ggatcttggg aagattaaag gcagcgtcga
    18061 gagaataatt tagtgcaata gatgcctctt tggcctcttg ttgggctcgc ctatccagcc
    18121 atgtgatcgc cggggcaagc ggctcgcctc tttcatcaat aggaagaata gtaggcccat
    18181 tgccacatat gacaatagct ttgagttcat tcttttctcc cgtcgctatc aaacgagaga
    18241 tgatctcaga gaatgcctta agccaaaggc gcgcatttat ttcctcatag taattagaac
    18301 tttcatctgg aagatctact atttctaatt tgaggcttct accttccgca tcgaaaagcg
    18361 cagccttgat tcgagtggta ccgatatcga cagcaagaat caaaattcaa ctcctatgga
    18421 ataccattaa taagcaagcc ctttatatgc atttccttaa cttgctggat gaaagatcct
    18481 ttacaataaa gggggcatgg agggctgagt ttaccacaac atagctagaa atgcatagaa
    18541 caacaatagc tggttttttc atcatctcat ttccttgtca ggattatgct ctgaatcaat
    18601 acagaaggta ttgaaattgc acactttgca ctcctcacca gggcaagaga agaaagactt
    18661 cttttctttt atcgctatga tgttcgcttc gatttgagca atacggcgct gtgcctcaag
    18721 gattctagtt tcttcttcct ttttttggtc ttcgtagttc tgtagaagaa gaagtcggca
    18781 tttttcgcca cttggatcct ggctatatag gatgaaaaac tcgcgaattt ggccaagagt
    18841 gagacccatc gctttaagct cttctatcct cttcaatcgg ataatggcat cggctggata
    18901 gcgtctatgg aatccttcgg tgcgattgct tgcttctatg agaccgagtt cttcatagta
    18961 gcgaatggtt cgcgccgtga ctctaaatag cgaggcaagt tcaccgatct ttagaagctt
    19021 atccggcaat gtgtacaccc cttgaacata ttattaagat cgctatacta aatctaacgt
    19081 gagattttat ctcacgttag attttaagct atgtaagatt ctatcatatt tcctgccctc
    19141 cagggtatag caggaggaat cccatgcgcc gatttataat cgcatctctg gttgctattc
    19201 tgacgagtac cgcactatgg gcacaagccc ctagaatagg cgttttcgtt cctggcgctc
    19261 gcgacggaag tcccatctac gataatatgg caaaaggagc ggaaaggctt gtctctgaaa
    19321 cgcctggagc aagcctaaga gtcacagaag caggttttaa tcaagccgaa tggaaagaaa
    19381 aactcatctc atttgtcgcc tctggcaaat tcgatatcgt catcacctcc aatccttcga
    19441 tgccagagct ggtaaatgag gcttccaaga tctttcctaa acaaaggttc atttgcctcg
    19501 atggctatct tgcgggcaat tccagtgtct attcagcact atacaatcaa ctcgaacaag
    19561 gctatgtcac cggatatctc tcagggcttg tctcgatatc tggcatgaag ggaacaaacc
    19621 cagaaaaaaa aatcggcatg ataataggtc agaattatcc ggcaatggaa aagatgatca
    19681 ttccgggttt tctgaaagga ctgatggctg tcgatccagg ttttcagctg gatatccgtg
    19741 tgctcggaaa ctggtacgat gcagcaaagg cagcggaact gagccgcagt ctatacgctc
    19801 agggtgcaga tatcatactg ccaatctgtg gttcggcgag ccagggcgcg gtcagagttg
    19861 ctcaagagac tggcaagtat ctcgtcttct tcgatgacga tgaatt
//
LOCUS       DAOMZL010000029        19488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_2467_length_19488_cov_15.945711, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000029 DAOMZL010000000
VERSION     DAOMZL010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 19488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19488
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2467_length_19488_cov_15.945711"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1880
                     /gene="dnaK"
                     /locus_tag="PLQ30_03935"
     CDS             <1..1880
                     /gene="dnaK"
                     /locus_tag="PLQ30_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPW01626.1"
                     /translation="EGGEPIVIANSEGQRTTPSVVAFTAKGERLVGQPAKNQMITNAE
                     NTVFSIKRFMGRRYSEVLNEIKLVPFKVIDNGNDVRIEAGGKLYSPQEISAFILQKMK
                     KTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRIAGLEVKRIINEPTAASLAYGFN
                     KDQKKEKTIAVYDLGGGTFDISILELGEGVFEVKSTNGDTHLGGDDFDRRIVNWLVEE
                     FKNDTGIDLSHDRMAVQRLREASEKAKIELSNMQQTEINLPFITADANGPKHLQKSLT
                     RARFEQMCMDLIERSREPCIKALADAGLSASQIDEVILVGGMTRMPRVQQMVREIFGK
                     EPSKGVNPDEAVAIGAAIQGGILGGDVKDVLLLDVTPLSLGIETLGGVFTRLIPRNTT
                     IPCRKSQIFSTAADGQTAVSIHVLQGEREMAAQNRTLGRFDLVGIPPAPRGVPQIEVT
                     FDIDANGIVHVSAKDLGTGKEQKIRIEASSGLNEAEIEKMVKEAEAHAEEDRRERERI
                     DARNEADSLIYSTEKSLKEVGDKISASEKSAVENAVKELKDVMEGKDTELIKQKTEAL
                     KQASYKLSEELYRSASAQGAASEAAGSATGESASDGSAGSSDNAQNADDVDYKVVDDD
                     K"
     gene            1946..3070
                     /gene="dnaJ"
                     /locus_tag="PLQ30_03940"
     CDS             1946..3070
                     /gene="dnaJ"
                     /locus_tag="PLQ30_03940"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPW01627.1"
                     /translation="MAKRDYYEVLGVPKTATKDEIKKAYRKLAIQYHPDKNPGNKTAE
                     EKFKEATEAYEVLGDDQKRQAYDQFGFAGVEGMAGAQQDYSSVFRDFEDIFSGFGDFS
                     SFFGSIFGEGSQRRGETRTNHGANLRYDIELPLEKAVFGTTVEISYNRDDVCKTCGGS
                     GSRDNLGRKVCSTCKGTGQVRRSSGFFAISQPCPACHGEGTVIENPCQDCAGSGVVKK
                     KQKIRVTIPPGVEDGKRVTVPGQGNAGPNGGPPGDLHVFIHVRPHETFERQGNDLYCA
                     VSIDFVTAALGGEVVIETLEGRKISVQIPAGTQNAKLLRIKGEGVPVSGGKRGDLYIK
                     VFVKVPSRLTKRGRELLEELKKIEGETLQPELVKLKDIPR"
     gene            complement(3116..3325)
                     /locus_tag="PLQ30_03945"
     CDS             complement(3116..3325)
                     /locus_tag="PLQ30_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HPW01628.1"
                     /translation="MKKLLKIEGMTCGHCVMHVQSALEAVPGVRSAKVDLLQRQAIVE
                     GEQLDDQALRAAVTEAGYRIVEIIP"
     gene            complement(3650..5755)
                     /locus_tag="PLQ30_03950"
     CDS             complement(3650..5755)
                     /locus_tag="PLQ30_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW01629.1"
                     /translation="MAESNAKRHNILSHLRQLRPIEHFWLVVFFIILLGTLVIGAYCQ
                     WQNILHDKKLYIELIGKSKISQIEIWYTAHITEAKEKPHSLPFLNLVSRAIAEPSTDN
                     LELLDESLRPSLLSYNYADTAVLTSSFDVLYTLTNLSTSNSSEVVREISERKPRTPFF
                     TRMYLTSPHAKAGFHLVIPLIWEGEEEPFAYVVHTIFAENYFFPILATWPGTEETGEV
                     MLLESSNNMVQVINPLKLVPISAFSLQISIAAENSVEAQAGLGKTGVMMGKDYRGKQV
                     LAYAEKIPNLDWIILSKLDWAEAFASLIPTIVVFIIFIFVAIIALLAGSYAIFSTRAL
                     ATFQSKFELLKRTERNEALLSTILERLDFPVVAVDENLEIRLANHSFRDRFGDTLPKD
                     LQLAHKDTKTAQTEVQRIEIAGPQGQTLRFDSTEIQIALPDQPMLFVYVMRDVTNLET
                     MLEQVNNLNRELAQKVEEQTKCLSDTNDEWRAIASAISYNLIAPLYNIESLSKSLEAL
                     AKNKSDSEIMSYITNIRKTTESMTRLTEALSIFLSIDTIQLADEEFDFSIAAQEITSE
                     IIKRNPKRRYQITIMPDLKVHGDGNLLKIALRNVLENAIQNCPETLTAMIEIGKCGES
                     CIFVQDNGIGISPEEVYSLLKPFSNTENGQSAGQINVDFAITKKIIERHGGKLEIISE
                     LDKGTVVRFDFSSKSTTLR"
     gene            complement(6022..8025)
                     /gene="tkt"
                     /locus_tag="PLQ30_03955"
     CDS             complement(6022..8025)
                     /gene="tkt"
                     /locus_tag="PLQ30_03955"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPW01630.1"
                     /translation="MNIAALQAIALSVRSLTIDAIQKANSGHPGMPMGAAELGAYLYG
                     VELKYNPKDPKWLNRDRFVLSAGHGSMFLYSLIHMAGFDLSLDDIKAFRQVGSKCPGH
                     PEYGRTPGVETTTGPLGQGIATAVGMAIAERKMMAHFNTPGHQILDHFVFALAGDGCM
                     MEGVASEACSLAGHLGLGNLIVYYDSNHISIDGPTDITFSEDVGRRFEAYGWQVLRGS
                     MYNFEDIASLTAEAKAEKDRPSLIILDSIIGKGAPTKQGTSGVHGSPLGDEEARKTKE
                     GLGIPLDKPFWVAPEAYSYFKEHQGELEKEYDAWQLRFEAWQKANPSLAQELTIWLSG
                     KEMHKLDMPSFAVGDKVATRNASGKCLAAISTAWPNFIGGSADLTNPNVSQLPQNTDG
                     TPLVFSKTNPMGRYIYFGVREHAMAAIANGIALYGGLRPFVATFLVFSDYLRPALRLS
                     ALMRLPVIFVLTHDSIYVGEDGPTHQPIEALAALRAIPNLKVLRPADAEETSASWKIA
                     MERLDGPTVLVLSRQNLPVLEKHESDWSSKIRLGAYVVKDSDRAPEVTLVGSGSEVSL
                     AISAAEIVKSKRHEIAIRVVSVPDRGAFYASCPTVREIILSPGSKVFVAEAGVAQGWE
                     RIAPSDQILSIERFGESGPGDKVAEYLGFTAQTLARMILAKLD"
     gene            complement(8206..10113)
                     /gene="uvrC"
                     /locus_tag="PLQ30_03960"
     CDS             complement(8206..10113)
                     /gene="uvrC"
                     /locus_tag="PLQ30_03960"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPW01631.1"
                     /translation="MNARYAKKCDDKKSPQARDRDAIREAIQPLKGPIVETDSERRAR
                     LKALVKQAPECPGVYLMRDENHEVIYVGKAKVLKNRLSSYFAGKKDAKTRHLVSHIHE
                     IEWVLAAHEYEALLLENNFIKEYSPRYNVSLKDGKTYPSIRITNEEFPRVFRTRRIIN
                     DGSEYFGPYPSAETIDTYLDLIKRMFPLRRCANIKKHTSPCMYYHIGRCLAPCADKIS
                     LEEYTKQVDKVRKLLLGETESLVKDLEHDMNRAAERLRFEEAARLREAIRALRSFAGR
                     SNVVDFNSPGARDYIAWEADGDMASYVIFQMRDGKLKNRDSFMEHCVDELEALDSFLA
                     RYYDTGRLPPPQVFVRRADNTATIARYLSDRAGTPVEVKIPDSERHWAVMNMAVQNAK
                     EELAKRRRAHGDIVALVELKERLGLGSVPMRIEGFDIAQLGGKHTVASLVSFIGGVAE
                     KKNYRYFRIRSVEGKVDDFAAMREAVARRYTRLINEEAELPDLILVDGGLGQVSAAKE
                     ILDDLGLDIDIIGLAKRNEEIWLPDKNDPVVLPKDSPSLRILVAVRDETHRFATGLSR
                     KLRSSDATLHMLQEVPGIGDRRAKKIFNALGSLEAVAEAEPGYLSSIARVNEDTARKI
                     IELARRNVGIE"
     gene            10141..10884
                     /locus_tag="PLQ30_03965"
     CDS             10141..10884
                     /locus_tag="PLQ30_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPW01632.1"
                     /translation="MEKTKQQSEASQTPREPFAIMASSELPIIDIDNNGCSDEYIRSL
                     EEKTAHALERLGGIGAAASAFHFCPSCGSPKLYSVRSRMWKCPTCGFEYFHNVATAAG
                     VIIEAKNKIIMIRRHKDPMRGRFALPGGFTEPGERAEDAALRECYEEIGWSPTHINFL
                     ATFPNVYQYQGIPYATCDVYFYSRGNIVEIDDFHLDPEESDSVQFVAANAIPWANIAF
                     ESTIRALRYFILHESIGTPPRLPEFGAIE"
     gene            complement(10963..11949)
                     /locus_tag="PLQ30_03970"
     CDS             complement(10963..11949)
                     /locus_tag="PLQ30_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPW01633.1"
                     /translation="MDFVRWGILSVSNHYRLRVHHQLSGSKLTKMFGIASRDLEKAGQ
                     AASELGIARSYGSYEALLEDPEIDAVYIPLPNHLHAEWIKKAAEAGKHVLCEKPFAMN
                     AAQAQDALLYAESKGIKVMEAFMYRFHPQWVHAEEIVRAGEIGKLQYVHVQFTYNNRD
                     PRNIRNILSAGGGAIYDIGCYACSSSRFIIGKEPRRAISLVRRDVEFGTDVLSSAILD
                     FGDAEALFTVATQSFPVQRVDIVGSAGAIAIFIPFNMYDDIPAEMSISTSIGSRIVRL
                     GPAGQYRLMFDAFSKSIIEGRAVPTPPEDAIANMKVIDALFKSERSGFWEDV"
     gene            complement(11974..13158)
                     /locus_tag="PLQ30_03975"
     CDS             complement(11974..13158)
                     /locus_tag="PLQ30_03975"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW01634.1"
                     /translation="MNELEKQQNISRRFGLGYFMLFAMYGISSPYLQLMVRRLGYSPA
                     TVGFFLGFFELVGITGPIFIGQIADRFGKLKPFLYASAIMILVGLALLAPFRLPLVTL
                     LSLALLSIGIKTPIPVLDTSLLRAIEYSEEKGEKAPNYGIIRAIGSAGFVITALIAQS
                     IPGFDSSSTGTMAIPLGILALLFLLSIPSLPEIGAGQARKEKIKFSFKWLDATYLAGL
                     GVIALGRLAMAPISSFFSMYLMEELNWHAVGSMWALSAACEIPFIVLSWKLIQGRSPM
                     MAIAISSVAIVVRLLIYALFPTPIGVILGQLLHSICYGLFQPAAILFINLKTPPSDRT
                     TGMALYMGVGMGLPAFLGSALGGTVVEIFGYRWLFASYTLFAVASILLFWRFRKELKA
                     VR"
     gene            13311..14690
                     /gene="wbaP"
                     /locus_tag="PLQ30_03980"
     CDS             13311..14690
                     /gene="wbaP"
                     /locus_tag="PLQ30_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate galactose
                     phosphotransferase WbaP"
                     /protein_id="HPW01635.1"
                     /translation="MIAGLLLGDLFAVMASFCSGFFLENLIDIEGINFKSFVTYWPYL
                     PAFIVMLSLLNLYPGITLSTPEKLRRFTVASFLAHAGIILSLFIGAKRFDQYQTAFLF
                     SWVFSIPLFFYCRTVSESLLVRYRLWGIPAVIFGAGKTGKLVVDRLLDNLKIGYIPVL
                     LLDDNPLLAGEYRGVPIVTGTNYGSFIASSGEVQTAIIAMPGVEHKRLTEIIVRQAMG
                     FRRYLIIPDVIGISSLWMAVQDLNGILGLFTDQELLEPRNIAIKRLLDIVLSVIGMIL
                     LSPFFIVIALLIKLDSPGPIFYSHHRLGKNGKPIEILKFRSMYTNSAQMLETLLRDNP
                     ELQTEWQANFKLKNDPRITRVGAFLRRTSLDELPQLWNILKGDLSLVGPRPIIDNEVE
                     KYGDNYWLLSKVKPGLSGLWQVSGRSETDYEQRVALDVYYIQSWSLWLDLYILLKTIV
                     VVLKGDGAY"
     gene            14677..15501
                     /locus_tag="PLQ30_03985"
     CDS             14677..15501
                     /locus_tag="PLQ30_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01636.1"
                     /translation="MVHIKFSKVRIFFFIILILVAAGIVYADSKPSLKDVLPGLTNDE
                     MRNLLASNLLIEDYAKDGSSRFLPSDQIAMTLGESLADRKDGNKYETAYLINDIRFSG
                     NDKLMFFNKLAQINTLSGIMYYSETRGKDTILFDNVYIVDAPGSKTPQQPRFFSQIPE
                     RLSLFVHIKDVNFGSIWFSILLFEKEDVFFFLIENVQPMGIGPIRAFDKGDFRIAFSF
                     KEIQEGLLVSSVSYFNPRRLASTFVDVFSAAEKRIRAISSWIAAQAIEAQNTPGHR"
     gene            15498..16958
                     /locus_tag="PLQ30_03990"
     CDS             15498..16958
                     /locus_tag="PLQ30_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HPW01637.1"
                     /translation="MSTELHEPKPSIISNFLFSTLSTFLTILIPLLTYPYVARILGPE
                     NLGRTGIASSFTNYFIAAAQIGLPVYGVRLVAQRRSDKKALERAVSELVQLSIASSFL
                     AILAFSIAVMAIPKYRSEWNLFLVFGLTIMTSAMNMDWLFQGMERFRYIGVRNAILQV
                     LFVVSLFLFVRKASDYIVYAFLFFAVGFATMIINYAVARRNIKLGFKDLSPLKHIGSI
                     SVITLFSFLVTAYTNLDFLFLSLFSRIEEAGFYSISIRIARIVIAFMATLSTVLMPRL
                     STLAETDKEESTRLLQNSFSAIALFVIPAAFGLVAIADDLIKVFAGAQFTPAVTSMRI
                     TAWIIPIVAFSNALQMQILIPQQKEKSMLVSFSIGILVTGLVLCFTVKPFGQAGAAMG
                     MLAGEASVLVAHAYFCGRKAILTIFARKRLFHYLVGGVLCGLVAYCISQLSGVMLIAR
                     LILAIAISAFFYFIFLLVVKDDFAILIIKGRHKNTG"
     gene            16961..>17952
                     /locus_tag="PLQ30_03995"
     CDS             16961..>17952
                     /locus_tag="PLQ30_03995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPW01638.1"
                     /translation="MEKTLNFEQNSGKFQSAHGLRSRKIIIISPNGLSDAGGVERVML
                     YAYRALSEHGFRVDIIDQKALSGSNIGRFFSFFKTGPLDFLAQSFGLSILANREHRSG
                     SFIINNGYSAFFAKSDILFCHGCIRGARLAQYRVRSEQQPSSLSERRAQIDGHKTSRI
                     TRLIGSLKIFLYGPDEIAEMIAGHRSHIILAVSEHSQREWQRYYRLSSSKFKNLPNSV
                     DTEHFQPDLESHKQQSSRQSRIALRILFIGSLTYLKGLDRLGATIEKARQTAKNWEFI
                     IATPSSFNTEKFASKQNVTIRKGVSFAELPDLYRSCDVMYFPSIYEGFEMVTLEA"
     assembly_gap    17953..18052
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18103..19044
                     /locus_tag="PLQ30_04000"
     CDS             18103..19044
                     /locus_tag="PLQ30_04000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPW01639.1"
                     /translation="MIEPCISVIIPTYKRAYLLSLTLPTYLQPETKEIIVVDDCSPDT
                     TREVVESLAADDPRIRYIRTDTNRKQPHAKNLGISHAKGQYIYFGDDDSVLLPGSLRR
                     LISTLHEYDASIVGACAPYLTRLTNDKDLSSIFEEISREMGQSERLYKAEIHKRLISP
                     VSLEAHFNARTPQPVEVPFVHASFLIYRELAEKTRFDEHYSGNCYREETDFLIRTHDA
                     GARIIYEPRAVQANLPRSIAKGGAHDGSSNFLIRKLRYFKSALENNWYFLTKNKGPLR
                     RAFGLNISPLHRQAVFAMIILRVIASYPIRKVIGHDL"
     gene            19034..>19488
                     /locus_tag="PLQ30_04005"
     CDS             19034..>19488
                     /locus_tag="PLQ30_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01640.1"
                     /translation="MTFNRNAIERNEPRALYVRGERVRLIRGGADHVSSSLLCMLRRY
                     FLNSIDELYLDEFCERTKSRRSVLFDALQGWFFPYGRKEAIQFRRILHGYDFVFLDQS
                     VYGRLLSDAKACRPDLKTAVFFHNVECEFYAQRIWHAGKIHNALLLPSIL"
BASE COUNT         5094 a         4594 c         4568 g         5132 t
ORIGIN      
        1 tggagggagg agagcctatt gtaattgcca attctgaagg acaaaggaca acgccctcgg
       61 ttgtagcctt caccgcaaaa ggtgagaggc tcgtaggcca gccagccaag aaccaaatga
      121 tcacaaatgc agagaacact gtattctcga taaagagatt catgggtcgt cgctatagcg
      181 aagtattgaa tgaaatcaaa cttgttccat tcaaggtcat agataatggc aacgatgtgc
      241 gcatcgaggc tggcggcaag ctctacagcc ctcaggagat tagcgccttt attcttcaga
      301 aaatgaaaaa gaccgcagaa gactacctag gcgagccggt tacggaagcc gtaatcacgg
      361 ttcctgcgta cttcaacgac tcacagcgac aagctaccaa agatgctggc cgaattgccg
      421 gtctcgaggt aaagcgaatc atcaatgaac ctactgcagc aagccttgct tatggtttca
      481 acaaggatca gaagaaagaa aagaccatag cggtatatga cctcggtggt ggtaccttcg
      541 atatctctat actggagctt ggagaaggcg tattcgaagt caagtcgacc aatggtgaca
      601 ctcaccttgg cggtgacgat ttcgaccgca gaatcgtgaa ctggctggta gaggaattca
      661 agaatgatac gggcatcgat ctttcgcatg atcgcatggc tgttcagcgg ctacgcgaag
      721 catcggaaaa ggcaaagatc gagctctcca atatgcagca gaccgagata aatctgccct
      781 ttataacggc cgatgcgaac gggccaaagc atttgcagaa aagccttacg agggctagat
      841 tcgaacagat gtgcatggac ctcatagagc gatcccgcga gccttgtatt aaggcgcttg
      901 cggacgcagg gctttcggca tctcagatcg atgaggtaat cctggtaggt ggaatgaccc
      961 gtatgcctcg cgttcagcag atggttcgcg aaatattcgg caaagagcct tccaagggag
     1021 tcaacccaga tgaagcggtt gccattggtg cggctatcca gggaggtata ctcggtggag
     1081 atgtaaagga tgtgcttctc ctcgacgtta cgccgctttc tcttggtatc gagacactgg
     1141 gcggcgtatt tacccgtctc attccgcgca atacgacgat tccatgccgc aagagccaga
     1201 tattctctac ggcagcggat ggacaaacag ccgtatccat tcatgtattg cagggcgagc
     1261 gcgagatggc ggcacaaaac cgaacacttg gaagatttga cctagttgga attcctcccg
     1321 cgcctcgtgg tgtgccacag atcgaggtga ccttcgatat cgacgcaaat ggtatcgtgc
     1381 atgtgtcagc aaaagatctt ggcaccggaa aagagcaaaa gatccgaatc gaagcatcga
     1441 gcggtctcaa tgaggcggag atcgaaaaga tggtcaagga agccgaagcc catgccgagg
     1501 aagacaggcg tgagcgggaa cgcatcgatg cgcgtaatga ggctgattct ctcatctata
     1561 gcacggaaaa atcgctaaag gaagttggcg acaaaataag cgcaagcgaa aagtccgcgg
     1621 tcgaaaatgc cgtaaaagag ctcaaggatg taatggaagg caaggatact gagctaatca
     1681 agcagaaaac cgaggccttg aagcaagcat cctataagct gtccgaagaa ctgtaccgaa
     1741 gtgcttcggc ccagggcgcc gcaagcgaag cagccggcag cgccacagga gaaagcgcct
     1801 ccgatggttc cgctggctca agcgataatg cgcagaatgc cgacgatgtc gactacaaag
     1861 tggtcgacga cgacaagtaa cctagagggc atatcgaggt ggctgggccg cggttcagcc
     1921 acctttttat caaaggattt tcgaagtggc caagcgtgat tattacgaag tgctcggtgt
     1981 gccaaagacc gcaacaaaag acgagatcaa gaaggcctat agaaaactcg ctattcagta
     2041 tcacccggac aagaatcctg ggaacaagac cgcagaggaa aaattcaagg aagccacgga
     2101 ggcatacgag gttctgggag acgaccagaa acgccaggcc tatgatcagt tcggctttgc
     2161 tggtgtcgag ggaatggcgg gagctcaaca ggactattcc agcgtatttc gcgacttcga
     2221 ggacattttc agcgggtttg gcgatttttc ttcgtttttt ggttcgattt ttggcgaagg
     2281 gtcgcagcgt cgtggagaaa cacgtacaaa tcacggtgct aatttaagat acgacataga
     2341 acttcccctt gagaaggcgg tttttggaac caccgtagaa atatcataca acagagatga
     2401 tgtctgcaag acctgcgggg gcagtggttc gcgcgacaat cttggcagaa aagtttgttc
     2461 tacctgcaag gggacagggc aggttcgcag aagttcgggt ttttttgcca tttctcaacc
     2521 ctgcccagca tgtcatggag agggcacggt aatagagaat ccttgtcagg attgcgccgg
     2581 aagtggagta gtcaagaaaa aacagaaaat tagagtcacc atacctcccg gtgttgaaga
     2641 tggcaaaagg gttactgttc ccggccaggg gaacgctggt cctaatggag gacctccagg
     2701 agatctccat gtctttatcc atgtgcggcc tcacgaaacg ttcgaacgcc aggggaatga
     2761 cctttattgc gccgtctcga tagattttgt aaccgctgca ctcggcggcg aagtggttat
     2821 cgaaacgctg gaagggcgga agatatccgt tcagataccg gccggaacgc agaatgctaa
     2881 gttgcttcgc ataaaaggcg agggtgttcc agtttcgggt ggcaaacgcg gcgatctcta
     2941 catcaaagtc tttgtcaagg ttccttcccg gctcaccaaa cgagggcgcg agctcctgga
     3001 agagcttaag aaaatcgagg gtgaaacctt gcagcccgag ctcgtcaagc tgaaagacat
     3061 accaaggtaa tataagaagg ctgctcggaa aatcgggcag ccttcttatt atttattatg
     3121 gaatgatctc cactattcgg tagcctgcct cggtcaccgc agcccgcaga gcctggtcat
     3181 ccagttgttc accctccaca attgcctggc gttggagaag gtctaccttt gcgcttctga
     3241 cgccgggcac tgcttcgagc gcagactgca catgcattac gcaatggccg caggtcatac
     3301 cttcgatttt aagcaatttc ttcattgtct atcctccctt tctctagagc tctagagtcc
     3361 atcgatctga ttttctatat ctcctgagcc gaagtgcatt agttatgaca gaaacagagc
     3421 tcaaggacat ggcggctgcc gcaaaaattg acgaaagcaa aggcccgcca aaagcatata
     3481 acaagcctgg acactatcga ccgaaatatt ggtcacggca tttagcttgt tcaccgctag
     3541 atcatttgcg gcaacgcaag cggcgcatgt catacttttt attgcaaaag tcttgcgatc
     3601 agccatggag gactccatat aaatccatca agacaaatat atagtaattt tagcgaagtg
     3661 ttgtagactt tgacgagaaa tcgaatctaa ccacagtgcc cttgtcaagc tcacttatta
     3721 tttcgagctt tccaccatgt cgctctatta ttttctttgt aatggcgaag tccacgttaa
     3781 tctggcctgc ggattgccca ttctcggtgt tagagaatgg ctttagtaga gaatagacct
     3841 cttccggaga tattcctatg ccattatctt gcacaaagat gcaggattct ccgcatttgc
     3901 ctatttctat catggcagtc agagtctcag gacaattctg gatagcattt tcaagaacat
     3961 tgcgtaaggc aatcttcaag agattgccat ctccatgtac tttgaggtca ggcataatgg
     4021 taatctgata gcgccttttg ggattgcgtt tgattatttc cgaggtaatt tcctgagcag
     4081 cgatactgaa atcgaattcc tcatccgcaa gctgtattgt gtctatcgaa agaaaaatcg
     4141 aaagggcttc cgttagtctg gtcatgcttt cggttgtttt tctgatgttc gttatatagc
     4201 tcatgatttc gctatcagac ttattctttg ctagggcttc cagagatttg ctcaaggatt
     4261 caatgttgta aagtggagca atcaagttat aagagattgc cgatgcaatt gcgcgccact
     4321 catcatttgt atctgaaagg cactttgtct gttcttccac tttttgtgca agttctcgat
     4381 tgagattgtt gacctgttcg agcatcgttt caagattggt tacatcgcgc attacataga
     4441 caaagagcat tggctgatcc ggcagggcaa tctggatctc tgttgaatca aatctcaggg
     4501 tctgcccctg tggaccggca atctcgatcc tctgaacctc ggtttgagcc gttttggtgt
     4561 ccttatgagc caactgcaga tcctttggaa gggtgtctcc aaagcgatct ctgaaggaat
     4621 gatttgccag tcgaatttcg agattctcgt cgactgctac caccggaaaa tcgagacgtt
     4681 ctagtatggt gctcagaagt gcctcgttgc gttcggtgcg cttgagcagt tcgaatttcg
     4741 actgaaaagt ggcaagcgct cgtgtggaga aaatcgcgta tgagcctgcc aatagcgcta
     4801 ttatggcaac gaagatgaat atgataaaaa caacaatcgt cggtattagc gaagcaaacg
     4861 cttccgccca gtcgagcttt gaaagaatta tccagtctag atttgggatt ttttctgcat
     4921 aggccagcac ttgctttccg cggtaatcct tgcccatcat aacaccagtc tttccaagac
     4981 cggcttgtgc ctccacagaa ttctcggcgg caatggaaat ctgaagagaa aaagccgaaa
     5041 taggaacgag ttttaatggg tttatgactt ggaccatatt attgctgctt tctagcagca
     5101 ttacctcgcc tgtctcttcg gtgcccggcc atgtagcgag gataggaaag aaatagtttt
     5161 cggcaaaaat tgtatgcaca acataggcaa aaggctcttc ttctccttcc cagatgagag
     5221 ggatcacaag atgaaaaccc gcttttgcat gaggagaagt aaggtacatt ctggtaaaga
     5281 aaggagttct gggttttctt tcggaaatct cccgaacaac ttcggaactg ttagaagtgg
     5341 aaagattggt aagcgtatat agaacgtcga agctcgaagt gagcaccgca gtatcggcat
     5401 aattgtaaga gagaagagat ggcctcaagc tttcatcgag gagttctaaa ttgtctgtcg
     5461 aaggctctgc tatcgccctt gagactagat ttaagaaagg cagcgaatgt ggcttctcct
     5521 ttgcttccgt gatatgagcc gtataccata tctcgatctg actaatcttg gattttccaa
     5581 ttagttcgat gtagagtttt ttatcgtgca ggatattttg ccattgacaa taagcgccta
     5641 tcacaagagt accgagcagt atgataaaga aaacaacaag ccagaaatgc tcgataggac
     5701 gcaattgtct aaggtgagaa agaatattgt gcctcttagc attagattca gccactgttt
     5761 agactccttc tcattcgcta ttgccaaacc cttagtaaga gaaaggataa tctatactaa
     5821 cacagaggac ttttatttcc aagaatattg aatttttaca gtgttataat aactattttg
     5881 tttgacaatt atgattattg tgcttttttg atctaaagaa cattctggct ccaaccagcc
     5941 aagctcgaga tccgagcttg gcctaaagga gcactttcaa gtaatgatac aaatcaaagc
     6001 ataatatgta aagaggcttt tctagtccaa tttggccaga atcatccgag caagggtttg
     6061 tgccgtaaat ccgagatatt cagctacctt atctcccgga cctgattcac caaagcgctc
     6121 gatagaaaga atctgatctg aaggagcaat acgctcccag ccttgagcta caccagcttc
     6181 ggctacaaac accttagatc cgggagaaag gatgatctct cgaaccgtcg ggcaagacgc
     6241 atagaaagca cctctgtccg ggacagaaac aacgcggata gcgatttcat ggcgtttgct
     6301 tttgacaatc tcggcagcag agattgctag cgagacttcc gatccagatc ccacaagtgt
     6361 cacttccgga gcgcgatcgc tgtcttttac aacataagcc ccgagcctta tcttggaaga
     6421 ccaatcgctt tcatgttttt ctaggacagg cagattctgt cgcgaaagca caagcacggt
     6481 aggtccatcc agtcgctcca ttgcaatctt ccagctagcg cttgtttcct ccgcatccgc
     6541 ggggcgcagc acctttagat taggaattgc cctcagtgcc gcgagcgctt cgattggctg
     6601 atgcgttggt ccatcttcgc ccacatatat cgaatcgtgg gtaagcacga agattacagg
     6661 cagtctcatt agtgcggaga gcctcaaggc cggacgcaga taatccgaaa aaaccaggaa
     6721 tgtcgctaca aaggggcgca agccaccata tagcgcaatt ccattggcta tagctgccat
     6781 ggcatgctct cgcacgccaa agtagatata tcgccccatt ggattggtct ttgaaaagac
     6841 aaggggagta ccatccgtat tttgcggcag ttggctcacg ttggggttcg tcagatcagc
     6901 tgagccaccg ataaagttcg gccaggcggt agaaatagcg gctagacact tgcccgaagc
     6961 atttcgtgta gccaccttat cgccaaccgc gaaagaaggc atatcgagct tatgcatctc
     7021 tttaccgctg agccagattg tgagttcttg ggccagggaa ggattggcct tttgccaggc
     7081 ttcgaagcga agctgccagg cgtcatattc cttttccagc tctccttgat gttccttgaa
     7141 gtaagaatag gcctccggag ctacccagaa gggcttgtcg agtggaatgc caagaccttc
     7201 cttggttttg cgagcttctt catcgcccag cggagaacca tggacaccgc tcgtaccctg
     7261 ctttgtcgga gctcccttgc caataatgct atccaggatt atgagagaag gccggtcttt
     7321 ttcggccttg gcttcggcgg ttagagaagc gatatcctca aaattataca tggaaccccg
     7381 aagcacttgc caaccatagg cttcgaaacg cctgcctaca tcttccgaga aagttatatc
     7441 agtaggacca tcgatgctga tatggttcga atcgtagtag acaatcaggt ttcccaaccc
     7501 aagatgaccg gccaaagagc aggcttcgct ggctactccc tccatcatgc acccatcgcc
     7561 ggcaagcgca aacacaaagt gatcgagaat ctgatgaccc ggcgtattga aatgcgccat
     7621 catctttcgc tctgcaatag ccataccgac cgccgtcgca attccctgcc ccaagggccc
     7681 cgttgttgtc tcgacacccg gcgttcgccc atattccgga tggcccgggc atttcgatcc
     7741 gacctgacgg aaagctttta tatcatcgag agatagatca aagccagcca tgtggataag
     7801 agaataaaga aacatcgagc catgaccggc cgagagaaca aatctatccc tatttagcca
     7861 ctttggatcc ttagggttat acttgagctc gacaccataa aggtaagcac cgagctccgc
     7921 agcgcccatc ggcatgccgg ggtgacccga atttgccttt tggattgcgt cgatagtcag
     7981 gctccgaaca gaaagagcaa tagcctgcag tgcggcaata ttcatcgaaa tcctcctaaa
     8041 aatactagat cttgacaaga ttcattggat atccttgact ggattaacag gacaattcga
     8101 acagaattga tgggatttta tcccaagact ctcttatccc gttcatccgg cctcattgcg
     8161 ttatcctgtc caagcatcct atcatcgagt cgaatctttg taaaactact caatacccac
     8221 gtttcgtcgg gctagctcaa ttatctttcg cgcggtgtct tcgttcacgc gagcaatact
     8281 gctaaggtat ccgggctctg cctccgcaac cgcttcgagc gatccaagtg cattgaaaat
     8341 ctttttagcg cgtctatcgc caatgccagg aacctcttgc aacatatgga gggtagcatc
     8401 gcttgacctc agtttacgcg aaaggccggt ggcaaagcgg tgtgtttcat ctcgtactgc
     8461 gacaagaatt ctcaaagaag gtgagtcttt aggtagcact accggatcgt tcttatcggg
     8521 cagccatatt tcctcatttc gttttgccag gcctatgata tcgatatcaa ggccgagatc
     8581 gtccaggatt tccttggccg ccgagacctg acccaagcct ccgtcgacga gtatgaggtc
     8641 cggcaactcg gcttcttcgt tgataagcct ggtgtatctg cgcgccacag cctctcgcat
     8701 ggcggcaaaa tcgtctacct tgccttcgac ggaacggatt ctaaaatacc tataattctt
     8761 tttttctgct actcctccaa taaaactcac tagcgaagcc acagtatgct ttccaccaag
     8821 ctgagctatg tcgaatccct cgatacgcat tggaacagat cctagcccaa gcctctcttt
     8881 tagctcgaca agagcaacta tgtctccatg tgcgcggcga cgctttgcca gttcttcttt
     8941 tgcattttga accgccatat tcatgactgc ccagtgtcgc tcgctgtccg gaatctttac
     9001 ctcgacagga gtacctgcgc gatccgaaag atatcgtgct attgttgccg tattgtccgc
     9061 tcttcgtaca aagacctggg gcggcggcag tctgccagtg tcataatatc gcgcaaggaa
     9121 actatccaga gcctcgagct catcgacaca gtgttccata aaactatctc tattcttgag
     9181 cttgccatcg cgcatctgaa agatgacata gctagccatg tcgccgtctg cttcccaggc
     9241 gatataatcg cgcgcgcctg ggctattgaa gtccaccaca ttcgagcgac ccgcaaacga
     9301 ccgcaatgct cttatggcct cgcgcaggcg cgcggcttcc tcgaaacgca accgctccgc
     9361 agcgcgattc atgtcatgtt ctaaatcttt gaccagcgat tcagtctctc ctaaaagcaa
     9421 tttccgtacc ttgtccacct gcttcgtata ttcctcaagg ctaatcttat ccgcgcaagg
     9481 agctaaacag cgcccgatat ggtagtacat acaggggctt gtgtgttttt tgatattggc
     9541 acagcgccgc agagggaaca tacgttttat taggtcgaga taggtgtcta tggtctccgc
     9601 gcttggatat ggaccgaaat actcacttcc gtcatttatg atccgccttg tacgaaagac
     9661 tcgggggaac tcttcattcg ttattcggat cgaaggatag gtcttgccat cctttaggct
     9721 aacattgtaa cgcgggctgt attcttttat aaagttgttt tcgagaagaa gcgcctcata
     9781 ttcatgtgca gccagcaccc attctatctc atgaatgtgc gatacaagat ggcgcgtctt
     9841 tgcatccttt tttccggcaa aatacgaaga tagtctattc ttgagtacct tagccttgcc
     9901 gacatagatg acttcgtggt tttcgtctcg cataagatag acaccagggc attccggtgc
     9961 ctgcttgact agcgccttaa gccttgcccg gcgttcggaa tccgtttcca ctattggtcc
    10021 cttcaagggt tgtatagctt ctcgaattgc gtctcgatcc cgggcttgtg gagatttctt
    10081 atcatcgcat ttcttggcgt atcttgcgtt catagtatat aattaaaata aggttcttcc
    10141 atggaaaaga caaagcagca atctgaagca tctcaaaccc caagagagcc ctttgccata
    10201 atggcgagct ctgaattgcc tatcatagac atagataaca atggttgctc agatgagtat
    10261 atccgctcgc tggaagagaa aacagcccat gctctcgagc gccttggagg catcggtgcg
    10321 gctgcctcgg cttttcactt ttgcccatcc tgtggcagcc ctaaactcta tagtgtaaga
    10381 agtcggatgt ggaaatgtcc gacttgcggt tttgaatact tccacaatgt tgcgactgca
    10441 gcgggcgtaa taatcgaggc gaagaataaa atcatcatga taaggcgcca taaagatccc
    10501 atgagaggca gattcgcgct tccaggaggt ttcaccgagc ccggcgagcg agcggaggac
    10561 gcagccctcc gcgaatgcta cgaagagata ggctggtcgc cgacccacat caattttcta
    10621 gccacatttc cgaatgtgta tcaataccag gggattccct acgcgacatg cgatgtatac
    10681 ttctattcgc gaggcaatat cgtggaaatc gacgattttc acctggatcc tgaagagagc
    10741 gacagcgtgc agtttgtggc tgccaatgcc attccgtggg caaatatcgc cttcgaatcc
    10801 acaattcgcg ctttgcgcta ttttatcttg catgagagca taggtactcc gccgcgcttg
    10861 cccgaatttg gcgccataga atagagcatt tcaagtcagg accttctttc tatggagggc
    10921 cagaaaaaga tagtcaatgc tcaaccctgt gctcgaaatc ttctagacat cctcccagaa
    10981 tccagacctc tcggatttga agagtgcatc gatcactttc atattggcaa tcgcatcctc
    11041 tggcggagtc ggaaccgctc gtccttcgat tatgctcttc gaaaaagcat cgaacatgag
    11101 cctgtattgc ccggcaggtc caagtcttac gatgcgcgaa cctatacttg tcgaaatgct
    11161 catttcggca gggatatcat cgtacatatt aaagggtata aagatcgcta tggctcccgc
    11221 agatccgacg atatcgaccc tttgcacagg gaacgattgc gtggccaccg tgaaaagcgc
    11281 ttctgcgtct ccaaagtcca atatagctga ggaaagcaca tctgttccaa attccacatc
    11341 tcgccgcacg agagaaatag cacgccttgg ctccttgcct attatgaacc tgctcgacga
    11401 gcaagcatag cagccgatgt catagatcgc tccacctccc gccgacagta tatttcggat
    11461 gttgcgcggg tccctattgt tgtaagtgaa ttgcacatgc acatattgta gcttaccgat
    11521 ttcgcctgcg cggacgattt cttcagcatg tacccattga ggatggaagc ggtacataaa
    11581 ggcctccatc acctttatac ccttagattc cgcatagagt agggcatctt gggcttgggc
    11641 agcattcatg gcaaaaggct tttcgcacaa tacatgcttg ccggcttcgg cagccttctt
    11701 gatccattcg gcatgaagat gattgggcag tggaatatag accgcatcta tttccgggtc
    11761 ctcaagcaaa gcctcgtagg atccatacga acgagcaatt cctagctcgc ttgcggcttg
    11821 accggctttc tccaggtctc gcgatgctat accgaacatc tttgtcagtt ttgagcctga
    11881 gagttgatgg tgcaccctta gcctataatg attggaaacc gaaagaatcc cccatcgaac
    11941 aaagtccata ccaacctcct ataacataca ttgctatctc acggccttaa gttctttcct
    12001 aaacctccag aagagcaaaa tgcttgccac cgcaaacagc gtgtagctcg caaatagcca
    12061 gcggtagcca aatatttcga ccacggttcc gcccaaggcg cttccgagaa aggccggcag
    12121 acccattcct acacccatat agagagccat gccggtagta cgatccgaag gcggcgtctt
    12181 cagattgatg aatagaatcg ccgctggctg aaaaagccca tagcaaatcg aatggagaag
    12241 ctgacctaaa atcacgccga taggcgttgg aaaaagcgca tatatgagaa gccttaccac
    12301 aatggcaacg gaagatatcg caattgccat catagggctt cttccctgaa tgagtttcca
    12361 ggacagcacg atgaatggaa tttcacatgc cgccgacaga gcccacatgg aacccacggc
    12421 atgccaattc agttcctcca tgagatacat cgaaaaaaaa gagctgatcg gagccatagc
    12481 tagccgccca agcgctatga cacccaaccc cgctagataa gttgcatcga gccacttgaa
    12541 cgagaatttg atcttttcct tccgtgcctg cccagcacca atctcaggca aagaaggtat
    12601 actgagcaag aacaagagcg caagaattcc caagggtatc gccatggtgc ccgtcgagct
    12661 agaatcgaat cccggaatag actgagctat cagcgctgta ataacgaaac cagccgagcc
    12721 aattgcccta ataatgccat aatttggcgc tttttctcct ttttcttcac tatactcaat
    12781 agccctcagg agagaagtgt ccagtacggg tatcggggtc tttattccta tggagagcag
    12841 ggcaagactc aagagggtaa ccaatggaag tcgaaaagga gctagcaatg caagaccaac
    12901 gagaatcatt atcgccgaag catatagaaa aggcttgagc tttccaaacc tgtcagcaat
    12961 ctggccaatg aatatcggcc ccgtaattcc cacgagttcg aaaaaaccaa gaaagaagcc
    13021 aaccgtagca ggactatatc cgagccttcg caccattagc tgcagataag gcgatgagat
    13081 cccgtacatc gcaaagagca taaagtaacc aagtccaaac cgccgcgata tattctgttg
    13141 tttttcaagc tcgttcataa ctacttgaaa gaatagggtt aagggagaga tgcgtcaaca
    13201 tttattgcaa caataccaag gaacatgtag aataatgatt gatggaaatg cctatcataa
    13261 gtcttttgca tgctcattgc ccttctaatc aaaagaccaa gaaattgctt atgatagctg
    13321 gactcctcct gggtgatctt tttgcagtca tggcaagttt ttgttcaggc tttttcctgg
    13381 aaaacctaat cgatatagaa gggatcaatt ttaaatcatt tgtcacttat tggccttatc
    13441 ttcccgcatt tatagtcatg ttatcgcttc tcaatctcta tcccggaatt actctttcca
    13501 caccagaaaa gctaaggcgt tttactgttg catcgttcct tgctcatgca ggcattatcc
    13561 tgtccttgtt tattggagcc aaaagatttg accaatatca gacggcgttt ttattttctt
    13621 gggtcttcag tattcccctt ttcttctatt gcaggactgt atcggaatcg ttgcttgtaa
    13681 ggtatagatt gtggggaatt cctgccgtca tatttggagc ggggaaaacg ggcaagctcg
    13741 tggtcgatcg acttctcgat aatctcaaga ttggctatat tccagtgctg ttgctcgatg
    13801 acaaccctct ccttgccggt gaatatcgtg gtgttcccat tgttaccggt actaactatg
    13861 gttcttttat tgcgtcttcc ggagaggtgc agactgccat tatagcaatg ccgggggtgg
    13921 aacataagcg tttgaccgaa atcatagtcc gacaagccat gggattccgc cgctatttaa
    13981 ttatcccaga tgtcatcggt atctcgagcc tgtggatggc ggtccaggat cttaatggaa
    14041 tcttgggctt attcacagat caggagcttc tcgagcctcg aaatattgct atcaagcgcc
    14101 ttttggatat cgtactcagt gttattggca tgatattgct ttctcctttt tttatcgtta
    14161 ttgcgctttt gattaagctc gattctccgg gtccgatatt ctatagccac cataggctag
    14221 gcaaaaacgg caagccgatt gaaatattga aattccgctc aatgtatacc aattccgctc
    14281 aaatgcttga aaccttgctg cgggacaatc cggaattgca gacagaatgg caagctaatt
    14341 tcaagctaaa aaacgatcct cgtataactc gggttggtgc ttttttgcga aggacaagtc
    14401 tggatgagtt gccgcaacta tggaacatcc taaagggcga tctcagtctg gttggtccca
    14461 gacccatcat tgataacgaa gtggaaaaat atggggacaa ctattggcta ctctccaagg
    14521 tcaagcccgg gctgtcgggt ttatggcagg tatccggtcg ttccgagacc gactatgagc
    14581 agagagttgc cctggatgta tactatattc agagctggtc gctctggctc gatctctata
    14641 ttctcctcaa aacaattgtg gtagtgctca aaggtgatgg tgcatattaa attctcgaag
    14701 gtcagaatat ttttctttat aattcttatt cttgttgcag ctgggatagt gtatgcagat
    14761 agcaaacctt cgctaaagga tgttttgccc gggcttacaa acgatgagat gcggaatctt
    14821 ctggcaagta atcttcttat agaagattat gcaaaggatg gttcctctcg atttttgccc
    14881 tccgatcaga ttgccatgac cctgggggaa agtctagcag atcgaaaaga tggaaataaa
    14941 tatgagaccg catatctaat caatgatatc cgtttttctg gtaatgataa gctcatgttt
    15001 tttaataagc tcgcacaaat caatactcta tcaggaatta tgtattattc tgaaacaaga
    15061 ggcaaggaca ctatcttatt cgataatgtc tatatcgtcg atgctccggg atcgaaaact
    15121 ccccagcagc caagattctt ttctcaaatt cctgagcggc tgagtctttt tgtacatata
    15181 aaagatgtca attttggatc gatctggttt tccattttgc tgttcgagaa agaagatgtt
    15241 ttctttttcc tgatcgaaaa tgtgcagccc atgggaatag gccctatacg cgcctttgat
    15301 aaaggagatt ttcgaatcgc gttttctttc aaggagattc aggaaggtct tcttgttagc
    15361 agtgtgagtt atttcaatcc aagacgattg gcttccactt ttgtggatgt tttctccgca
    15421 gcagaaaaga gaattagggc tattagcagt tggatagcag cacaagctat cgaggctcag
    15481 aatactccgg ggcatcgatg agtactgagt tgcatgaacc taagccttcg ataataagca
    15541 attttctttt ttcgactctt agtactttct tgaccatact cattcccctt ctcacctatc
    15601 cctatgtagc gagaattctt gggcccgaaa atctcggccg cacaggaatt gcttcctcat
    15661 ttacaaatta tttcattgca gcagcgcaaa ttgggcttcc tgtctacggc gtgaggctcg
    15721 tggctcagcg tagatccgac aaaaaagcac tcgagagagc tgtttcggag cttgttcagc
    15781 tttcgatagc ttcttcgttt ttggctatat tggctttttc tatcgctgta atggccattc
    15841 caaaatatag aagcgaatgg aatctttttt tagtattcgg attgactata atgacttccg
    15901 ccatgaatat ggattggcta tttcaaggta tggaacgctt cagatatatc ggcgttcgca
    15961 atgcaattct ccaggttcta ttcgtggttt cactctttct ctttgtgcgc aaggcctcgg
    16021 actatatcgt ttatgcgttt ttgttcttcg ctgttggatt tgctacaatg ataataaatt
    16081 atgctgtcgc tcgtagaaac atcaagcttg gattcaaaga cctttcccct cttaagcata
    16141 ttggttcaat ttcagtgatt acgctttttt cctttcttgt aacagcctat acaaacctag
    16201 atttcttgtt tttgagcttg ttttcacgga ttgaggaggc aggattctat agcatctcta
    16261 ttcggattgc cagaattgtc attgcattta tggcaacgct tagcacggtg ctcatgcccc
    16321 gcctttcgac gcttgcagaa acggataaag aagagagcac aaggcttttg cagaattctt
    16381 tctcggcaat agcattattt gtaattccgg cagctttcgg acttgtagca attgccgatg
    16441 atttgataaa agtatttgcc ggagctcaat ttacacctgc agtaacttcg atgcgaataa
    16501 cggcgtggat cataccgata gtggcattct cgaatgcgct tcagatgcaa atattgatac
    16561 cacagcagaa agaaaagtct atgctggttt ctttcagcat cgggatatta gtgaccggcc
    16621 ttgtgctgtg ctttacggta aagccctttg gccaggccgg cgcagccatg ggcatgttgg
    16681 cgggagaggc atcggtcctt gtcgcgcatg catacttctg tgggagaaag gcgattctta
    16741 cgatttttgc tcgaaaaaga ttattccatt atcttgtggg aggagtgctc tgtgggctcg
    16801 ttgcctattg cataagtcaa ttgtcaggag taatgcttat tgcaaggctc atcttagcca
    16861 ttgctataag tgcgtttttc tattttatat ttcttctcgt agtcaaggat gatttcgcga
    16921 tcctgatcat aaaaggtcga cataagaata ctggatagat atggaaaaga ccttgaactt
    16981 tgagcaaaat agtggaaaat ttcaatccgc tcacggactt aggtctcgaa agataataat
    17041 catctctcca aatgggctct ccgacgcggg aggagtagag cgagttatgc tatatgcata
    17101 tcgtgcgctt tcggaacatg gctttcgagt cgacataatt gatcaaaaag ccttgtctgg
    17161 aagcaatatc ggtcgattct tctcgttttt caagacagga ccattagatt ttttggcgca
    17221 atcctttgga ttgtccatac ttgctaatag agagcatcgg tcgggttctt tcataataaa
    17281 taatggctat tccgcctttt ttgcaaagtc agatattctt ttttgccatg gatgtattcg
    17341 aggtgcgcgc ttggcacagt acagagtgcg ctcggaacaa cagccgtcga gcttatctga
    17401 gcgcagggcg cagatcgatg gtcataaaac cagcaggatt acaaggctca tcggaagttt
    17461 gaagattttc ttgtatggac ccgatgagat tgccgaaatg attgccgggc atcgctcgca
    17521 tatcatctta gcggtatcag aacattcgca gagggagtgg cagcgctatt atcgcctttc
    17581 atccagcaaa ttcaaaaacc tcccaaattc cgtggataca gaacatttcc agcctgatct
    17641 agaatcccac aaacaacaat cttcgagaca atctcggatt gcgcttagga ttctctttat
    17701 tggatcgctt acatatctca aaggcttgga caggctcggc gcgactattg aaaaagcgcg
    17761 acaaaccgca aagaactggg aattcataat cgcaactcct tcatccttca atacagaaaa
    17821 gtttgcctct aaacaaaatg ttactattcg caagggcgtg agctttgctg aattacccga
    17881 tttatatcgt tcttgtgatg tcatgtattt tccttcgata tacgaaggct tcgaaatggt
    17941 cacgctggaa gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntggaattt
    18061 caacatggtc gcagaagctg atagagattc tggaagcata ccatgattga accatgcatt
    18121 tccgtcataa tacccacata taaacgagct tacctcctat ctcttaccct tcccacatac
    18181 ctgcagccgg agaccaagga aattatcgtg gtggatgact gttccccgga tactactcgt
    18241 gaggtggttg aaagccttgc agcggatgat ccaagaatcc gctatatcag gacagataca
    18301 aaccgaaagc agccccatgc aaaaaacctt ggtatatcac atgccaaagg gcaatatata
    18361 tattttggag atgacgattc ggttcttttg cccggctctt tgagacgact tatttcgact
    18421 ctacacgaat atgatgcttc gatagtcggc gcctgtgcgc cctatctcac aagactgacc
    18481 aatgataaag atctttcttc tatatttgag gagatttcta gggagatggg tcagtcagag
    18541 agattgtata aggctgaaat ccataagaga ctcatctctc ccgtaagcct cgaagctcac
    18601 tttaatgcaa gaacgccgca gccggttgaa gtgccttttg ttcatgcctc ttttctcatc
    18661 tatcgcgagt tggcggaaaa gactcgcttt gatgagcatt atagtggcaa ttgctatcga
    18721 gaagagacgg attttctaat ccgaacgcac gacgcaggcg caaggattat atacgaaccg
    18781 cgcgctgttc aggccaatct tccgcggtcg attgctaagg gcggagccca tgatggctcg
    18841 agcaatttct tgataagaaa actgcgatac ttcaaatccg ctttggaaaa caactggtat
    18901 tttcttacaa aaaacaaagg gcccttgcga agggcttttg gcctaaatat ttctccttta
    18961 catcggcagg ctgtgtttgc gatgataatt ctacgggtaa tcgcatctta ccctattcgc
    19021 aaggtaatcg gtcatgacct ttaatagaaa tgctattgaa agaaatgaac ctcgcgctct
    19081 atatgttcgt ggcgaaagag tccggctaat cagaggagga gcagatcatg tgtcgagttc
    19141 gcttctttgc atgctcagga gatattttct aaattcaata gatgagctct atctcgatga
    19201 attctgtgag cgaacaaaaa gtcgtcggtc tgttctattc gatgcattgc agggctggtt
    19261 cttcccctat ggaagaaaag aagcgataca atttcgcagg attttgcatg gctatgattt
    19321 tgtctttctg gatcagtcgg tctacggaag gctcctttcc gatgccaagg cctgccggcc
    19381 agatcttaag accgcggtat tctttcacaa cgtagaatgc gaattctatg cgcagcgaat
    19441 ctggcacgca ggcaaaattc ataatgctct gctgctccca tcgattct
//
LOCUS       DAOMZL010000030        18370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_2676_length_18370_cov_14.745236, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000030 DAOMZL010000000
VERSION     DAOMZL010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 18370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18370
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2676_length_18370_cov_14.745236"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..3384)
                     /locus_tag="PLQ30_04010"
     CDS             complement(4..3384)
                     /locus_tag="PLQ30_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPW01641.1"
                     /translation="MQGHYQRIALLLASAHTGAARGLWPELIAAAQSHRCILFVLPGG
                     RLDAVSGFEYMRNGVYRYAKASNVDAALCWASTLSGYATESAVESFLNDTIDVPLVTF
                     GLKIDGRPFVGIDAYSGMKALIGHFIRSHKRKHIAFIAGPRQHSSAEERYRAYCDALK
                     EASIPYDPRLVCLDTPWSEGRRAMLNLIDNRGLVPGKDFDALCTSSDLLAFEAGMLLK
                     ERGIDIPSTLPIGGFNDSEESRIFSPPLTTVRMPFIRQVLSALRLLIDMRNGNSADDI
                     LLKTKLLVRSSCGCMPQALQLAGRTRAKAPKSLPPSLIEAFGASVASQKNNSFFEYLN
                     KIFNEDRASSSLETGISENMMTALRFEFLSRASSREDIDKTERLIHQAMVALGLSKER
                     RHEYLLWKEKEDEQKLNLFNQELLCAKDVLSIIRAAAGHLPGLGILSSYLVTKDKQGN
                     LMFKGGYRKGKDTPYAELIYPRPSREAIPRGCFLPDRIIPTEPGAFFVLPLFFESTEI
                     GHLVIQAADIDPSLYEKIRSILSSALRGVKLFEEGDEARKRAERAEKSMSDIVSVISR
                     EIQSSIEAFDNISKLDATGAQEVEVSRLKELVRYLIDLSQAKSESVLARAVLFDPYCL
                     CARWAEEQLKADKSNNKGSNRAESRFICARPSSAYPLLYGDPKQLERMLTIVAQLLEE
                     TFSIEGLEISIVPSIQSCELRLASASMIPKKIGATGLNRIEKDIRFELAKQICLLHGG
                     YILFSHEGGNLIVRIGIPYPTVSGSIHNISNREAELRVIGPQGLSDHLPCGFVNGLSG
                     KIIPYNSIFHESINPLQRILLYLDSMEIDHESSAALSFILDKEKFQNAECFIAQNAFS
                     IRETRLEHRPADVAGFLRSILVSDSPSAILVLGSEASLHKETPIIRDLAQKPGIRPVA
                     CTSLDAFEAALNRWRPMLFLILEPMISKNQEFSTLLQRFPSIPIMAICRFFGAKEEWA
                     GIIERPHVVFCNTGEVFSEVLAGLIQRSLESKKFLPSPTSGVVADAIFYMNKSYSEQI
                     SRWKMASYLNASEDYLSRIFHAQMGISLWNYLARLRVQHAIDLLYSTGAGLAEIADLI
                     GFRDEAYFCRVFKRIAGATPGSFRSRSFSDVRKVQESD"
     gene            3540..4913
                     /locus_tag="PLQ30_04015"
     CDS             3540..4913
                     /locus_tag="PLQ30_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HPW01642.1"
                     /translation="MIVDIEEFGGIDDGCTDNSKAFADAMAALSETGGGTLRIMGSAY
                     LTGPIELLSNISLEIGKNTTLSFVSDINRYPPVLTRWEGVVCYGMHPLLFARGAKNIE
                     LKGEGTIDGNGQPWWEALRRKKRKGQNKPLESFETELAELNKYNGGQPSGGGGREIQF
                     LRPPLVQFFGCSDVAIEGLKFQNSPFWTIHPVFSNRISIRNISIHNPSDAPNTDGIDI
                     DSCKDILVTGSTIDVGDDCLAVKAGSGEQGISEGKSSERIVIRDCHFKQGHGGVVIGS
                     ETAGGIYEILVSDCSFDGTDRGVRIKTRRGRGGTVSRLRFSDLHMKRALCPVAINMYY
                     RCGAKLEECSKLFSLEHQPVDALTPHIHDIEISNLRAQECRASAGFIIGLPESPIENL
                     RMRNCRIELSRNDMVAPSESEMFEGLPEMQARGIRIRNANCDFDGLEVLGLPEGEAPY
                     FREPSPN"
     gene            complement(4928..6160)
                     /gene="corA"
                     /locus_tag="PLQ30_04020"
     CDS             complement(4928..6160)
                     /gene="corA"
                     /locus_tag="PLQ30_04020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPW01643.1"
                     /translation="MFSKKQNSAEPNTVCRASSEVGEGVAFEQSVFEKKASRESAQSN
                     SEKAKAAKPKHHASKLPFSGVSVETAGLPPGTPHYIGDIEPTLASYSLYSYDETSAVM
                     SIPDDISELLALIDLDVVNWININGLSGGIVEKLCGMLHIHPLVIEDILNTEHRPKLE
                     NYDDYLFLITKMITAHEDGSVEYDQVSFILKGKLLISFQERPGDCFKAVRDRIMSGAG
                     RLRKHGADYLLYALLDSIVDNYFIVLEMLGDMLERFEEASDSESDQEFFHGIQQLKRE
                     FSRMRRIIWPVRDSIAALSRTESIQMDVSLSPYLRDLYENTVQAIEALESYREHATSM
                     IELHLSQVNTNMSRVMTLLTILSAIFIPITFIAGIYGMNFAYMPELNKPWGYPAALLL
                     MAAVVIAELIYFKKKKWM"
     gene            complement(6346..7338)
                     /locus_tag="PLQ30_04025"
     CDS             complement(6346..7338)
                     /locus_tag="PLQ30_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPW01644.1"
                     /translation="MNKTAIRADLLLLLTAIIWGFAFVAQRVGMNYVGPFTFNAIRFA
                     LGSISLLPLIMYARKRNRKNGSRGLDSSEELDRSGEAGCEQRSMLNEDQTRATSKQKL
                     FLSGSFVAGSFLFIAASLQQIGIQYTTAGKAGFLTGLYVVLVPIVGMVWGRSTGLPTW
                     VGAILALVGIYTLSAPDKLGRVNSGDIFVLISAFFWTFHVLVIDNLAKRLDPVELAAG
                     QFAWCSVFSFAAAMIFEKISPNAVLDALVPILYGGLGSVGVGYTLQVLAQRDAPPAHS
                     SIIMCLEGVFATLGGILLLSESPSLRTMLGSVLMLLGMLATQWDVIFSGRSKQA"
     gene            complement(7398..9083)
                     /locus_tag="PLQ30_04030"
     CDS             complement(7398..9083)
                     /locus_tag="PLQ30_04030"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HPW01645.1"
                     /translation="MDLDISKTPWSAFGARFSVAIGKDDSVYLKDIGGGDEIPSELFS
                     MKLLLGKEGLDQKEELEQVIEANENECRIRAKNSPDRYVLLKFLFSDVISIECCDIRL
                     RLEMHKSSHDFIQEKAKGIFIIQSCAKDHVYELDLRDAVYNVEAPWGRAGNEYIRIDI
                     IKGVGSIREYALVPCESDTKNTKFFDFTQWNQNYTYTGLESRYCKARDLASYILWSAF
                     VRPEGKLTRNSVYSSKNWMTNIWSWDNCFCALAMVESMPDLALDQLLVFADNQHKAGA
                     YPDCINDRFSLFSFCKPPIHGWTLLEMIKRNPTILQSSSESIEYLYKSISESTNFWLR
                     FRRLPSGAIIYRHGNDSGWDNATLFMHGGPVESPDLYAYLSRQCDSLAEISRILEHGI
                     EEAQNWTFLAESLAESMLNRLWDDNGFFARKGLLGERIEHRDSFLLRLPIVANKRLPE
                     EVIKKLISDISDESRFLTPFGLATESLDSSFMEPHGYWRGPIWAPVNYLVFDALRLLG
                     ELNLANRIAQRFVDLCAQKGMAENFDPIHGEGLDDPAFAWTSAVFLLFLKESI"
     gene            complement(9088..9390)
                     /locus_tag="PLQ30_04035"
     CDS             complement(9088..9390)
                     /locus_tag="PLQ30_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01646.1"
                     /translation="MTDRQGFGLAIISEKPIEFSYHDYDVDALEKACYDHEIAHTAYC
                     ILNLDFAQNGIGSNSCGQDQLPPYRLKPQEFDLGLEFYALDPETSFLANAKSIGES"
     gene            complement(9413..9619)
                     /locus_tag="PLQ30_04040"
     CDS             complement(9413..9619)
                     /locus_tag="PLQ30_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPW01647.1"
                     /translation="MKQHNINAVRTSHYPNHPYFLELCDYFGLYVIDETDLETHGFEW
                     IDESTRLVNDPEYNPIYKTAGEWQ"
     gene            complement(9619..10401)
                     /locus_tag="PLQ30_04045"
     CDS             complement(9619..10401)
                     /locus_tag="PLQ30_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01648.1"
                     /translation="MNTSYGAECDWQNHKLLHIDRLPGRAYIKRWKDVQGALRDDESE
                     QLGFRNLNGSWKFLFLEAPELSPFGFEKPDFDDGAWEDIEVPSHWQLKGHGRPHYTDL
                     YYPFPVRPPFVPRANPTGIYRRQFFLDDLSRRQILRFHGVDSAFHVWINGKLAGFSKG
                     SRLLSEFDITSLVRVGKNVIVVRVYQWSDGSYLEDQDMWWLSGIFRDVEIYEVPLVSL
                     FDVSHEVSFDDIYKNALLKVELQIENKTEDMSPGGRCFKMFF"
     gene            10540..11286
                     /locus_tag="PLQ30_04050"
     CDS             10540..11286
                     /locus_tag="PLQ30_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPW01649.1"
                     /translation="MAAARRAIRIDDIYYSNRNDLALLSFDRSATIIIGSEGGCDISL
                     LNFLKKEKNAVCIDFDPDLKNIDVVCADFGKSIGTIAKRFAELGISRIGYIGGKEISP
                     LKGKEIIDPRSAQYIEEFGKLGIYRKEIFRAYGPYTPENAYQLTKELLSGTTKPEAIF
                     VASDNMALGVYKAICEANLSIPQDIKVISCNDQPVSAYMTPPLSTIRIPRAVMGSVAL
                     SLASSRAADFRELGVKVMVPTDVVFRKSFI"
     gene            complement(11355..12152)
                     /locus_tag="PLQ30_04055"
     CDS             complement(11355..12152)
                     /locus_tag="PLQ30_04055"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW01650.1"
                     /translation="MSFGYIHLMESLMNSLKGKKALVTGGASGIGRAISLELSNMGAD
                     LIIHYHKSRELAESLAEQIRTSGGKADVVQADLSEEKGVQILADFIFERWNNLDVLIN
                     NAGDLVGRYRLEDVRMDFYRKVVAVNLDSNVLVTRAMIPLLKRSGCASIVNLASLAGR
                     KGGHGGSIVYSMTKGAILTLTRALSVELAPYGIRVNAVAPGFILGSAFHAIHTSEESK
                     QATIRDIPLKRAGTCEDVARAVAFLASEYDGFITGATLDINGGVYMA"
     gene            12503..13474
                     /locus_tag="PLQ30_04060"
     CDS             12503..13474
                     /locus_tag="PLQ30_04060"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPW01651.1"
                     /translation="MKKKLIALAALLLITASFAIAAPKYNWKLASVLPDSHPVHQALV
                     FFADELARKSNGDIKVTLFPAGQLGQEADYIQAIKIGSLEFSKVSSGPLGQYAPTLQV
                     VSLPLIWRDLDHQHKVLQGQVGKRLMNDLDKAGFKGLVFMDAGFRSITAKKPIYTPAD
                     LKGLKIRVMQSKPLIDTINALGATAVPMGQSEVYSALQTNVIDGWENNEPTVLSFNMQ
                     EVAKYFSYTRHSSIPDILIMNKKLFDGLPKNIQTMIEETAFEAMQKHNQLWGEMIDKN
                     VAELKAKGMIFNEVKDITEFQKACAGVWKEFEPIVGKDLIDAIVNTK"
     gene            13582..14133
                     /locus_tag="PLQ30_04065"
     CDS             13582..14133
                     /locus_tag="PLQ30_04065"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPW01652.1"
                     /translation="MQDHQEGDMRKDLWHIVDRLERILTGFIAILMGIVTIVVCWQVF
                     DRYVLHKSPYWAEEFSVTIMMWMGLLGAACAVWTGDHMRLELLTKHLPARFGLILEIL
                     IDGAVIYFSYFLFKQGIELVNAMWTSQLSTIRLSIGITYLVIPISAAMMILFSILKIV
                     KKASGANAISSQQETKNGGEDAR"
     gene            14123..15403
                     /locus_tag="PLQ30_04070"
     CDS             14123..15403
                     /locus_tag="PLQ30_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPW01653.1"
                     /translation="MLAESVLLGSFIILILLGVPISFGLGISSLLSAFAAGLQPAIIA
                     QRVAAGMQSFPLLAIPLFILAGALMANGGVAKRMVDLAYVIVGPLPGGLAIVNCIDSM
                     FFGGVSGSAVADVSSTGPMIIPMMVEKGYDKDFAVALTVASASQGIIIPPSHNMVIYA
                     MVAGGVSVGRLFLAGYIPGMLLGIALAIACYFIALKRNYPCEKRPPLKESLRIILDGI
                     LALLCAAIIIVGIAAGIFTATEASAVAVLYAAFLGFFIFKELKLKDLWPIFMDSVKTT
                     SVVFFLIGCASAFGWMMTYLQIPLAVMNFFLSLTNNRFLILLLVDVLLLLLGMVMDVA
                     PLIVIVTPVLLPVVKAAGMSEITFGIVLMLNLGIGLTTPPVGASLFTGCMIGKTTIEK
                     ASKAMLPLWPAMLAVLFLVTYIPWFTEFLPNLFY"
     gene            15615..16943
                     /gene="thrC"
                     /locus_tag="PLQ30_04075"
     CDS             15615..16943
                     /gene="thrC"
                     /locus_tag="PLQ30_04075"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPW01654.1"
                     /translation="MLKPHLISTLSGASYPFQQLNEFAENGESLEVVIPNIEHARPLS
                     TSTGICDRICASSKRKAGTLGEPMARGTLIRTQNENAAASDASIWQRYADFLPFQETY
                     PELSLGEGMTPLVSAGNALSTWTGLDKLLLKNETVNPTWSFKDRGSLASAIMAIEQKE
                     RVMMTISTGNMGQSTAAYGTRAGLKVIVFLPEFVPQEKTLAMAIHGATVFRVHGTNYS
                     DMKEKVLMLASRHGIRVTSGNNPIRVEGYKTEAFELFEQLGGEVPDYIAVPTSACGHI
                     RGIFKGFRELHAAGLIDRLPKMIVVQAAANSPIASAIKSGSLTMIPFANFTTVAEALS
                     SGKPPGGDEIIAKAYQYGWLTEDATEEEIIEAQKRLAGAGFFVEPASATTLAALKKLR
                     ASGTIDAKSCVVAVLTGSGLKDTSVLSRHKLDIVDTDMHGIEKYISAERS"
     gene            complement(17384..17875)
                     /gene="msrA"
                     /locus_tag="PLQ30_04080"
                     /pseudo
     CDS             complement(17384..17875)
                     /gene="msrA"
                     /locus_tag="PLQ30_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882079.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
BASE COUNT         5075 a         4338 c         3984 g         4973 t
ORIGIN      
        1 ttttcagtca gattcttgta cttttcttac atctgaaaaa gatctagaac gaaaagatcc
       61 tggcgtcgca cccgcaattc gtttaaatac cctgcaaaaa taggcttcat cccggaaacc
      121 gattaaatct gcaatctccg caagcccagc tcccgtagaa tacagaaggt cgatggcgtg
      181 ctggaccctt agccttgcaa ggtagttcca gagcgatatt cccatctgcg catgaaatat
      241 tctgctgaga taatcttcgc tcgcgttgag ataagaagcc atcttccatc gcgaaatctg
      301 ttcgctatac gacttattca tatagaatat ggcatcagcg actacgccac ttgttggact
      361 tggcaggaat tttttgctct ccagtgatct ttgaatcagg cctgccaaaa cttcagagaa
      421 tacttcgccc gtgttgcaaa aaaccacatg ggggcgctcg ataattccag cccattcctc
      481 ttttgccccg aaaaatctgc aaatagccat gatcggtatg gaaggaaatc gctgtaaaag
      541 tgtgctgaac tcttggtttt tcgatatcat cggctctagg attagaaaaa gcatgggtct
      601 ccagcgattc aaggctgctt cgaaggcatc cagagatgta cacgcaaccg gccttattcc
      661 aggtttttga gcaagatctc gaataatggg tgtttcttta tgaaggctgg cttcgcttcc
      721 cagaaccaaa atggccgaag gagaatccga cacaagtatc gatcgcaaga atccagccac
      781 atccgcaggc cgatgctcca agcgagtctc ccttatagaa aaagcattct gggctatgaa
      841 acattcggca ttctggaact tctccttatc gagtatgaaa gagagagccg cggagctttc
      901 atgatcgatc tccatggaat ccaggtatag caatattctc tgtaaaggat ttattgactc
      961 atgaaatata gagttatagg gaattatctt cccagataat ccattcacga atccacaggg
     1021 caaatgatcc gacaagcctt gtggtcctat gacacgaagc tcagcctctc tattcgaaat
     1081 attatgtatg gatccgctta cggtgggata aggaatgcct atcctcacaa taagattacc
     1141 cccttcatga gagaatagaa tataacctcc atgaagcaga caaatctgct tcgctagctc
     1201 aaaacggata tccttttcga tccgattcaa tccggtagca ccaattttct tcggaatcat
     1261 agatgccgag gccagcctca attcgcaact ctgtatcgat ggcacaatcg agatttccag
     1321 cccttcgatg ctgaaggtct cttccagtaa ttgagccact atcgtcagca ttcgttcaag
     1381 ctgtttagga tcaccatata gaagcgggta tgcagaagaa ggtctagcgc atatgaatct
     1441 agattctgcc ctattgcttc ccttgttgtt cgacttatcg gctttcaatt gttcttcagc
     1501 ccaccgagca caaaggcaat aagggtcaaa aagcactgcc cgggcaagca cggattccga
     1561 ttttgcctga gaaagatcga tcaaataacg cacaagttct ttgagtctcg atacttcaac
     1621 ttcttgagcg cctgttgcat cgagcttcga aatattatca aatgcctcaa tcgagctctg
     1681 gatttccctg gatattacgg agactatatc cgacatggat ttctccgctc tctcggcgcg
     1741 ctttcttgct tcatcgcctt cttcaaacaa cttcactcct ctcagtgccg aagataaaat
     1801 cgagcgaatt ttctcataga gtgaagggtc tatatcggcg gcctgaataa ccaaatggcc
     1861 aatctcggtc gattcaaaga acaaaggcaa gacaaaaaaa gctcccggct cagtcggaat
     1921 aattctatcc ggcagaaagc aacctctagg gatggcttcc cgcgagggcc tcggatatat
     1981 caattctgca taaggagtat ccttgccttt tctataaccg cctttgaaca tcaagttccc
     2041 ctgcttatcc ttagtcacaa gataactcga gagaattccc aaaccgggta gatgtccagc
     2101 ggcggctctt attatcgaca gaacatcttt tgcacagagc agctcttgat tgaagagatt
     2161 gagtttctgc tcgtcctctt tctccttcca taggaggtat tcatgtcttc tttcttttga
     2221 gagccccaac gcaaccattg cctggtgaat tagtctttct gttttatcta tatcttctct
     2281 ggaagaagcc cggcttaaaa attcgaagcg aagtgctgtc atcatgttct ctgatatgcc
     2341 agtctccaaa gaagacgatg ctctatcctc attaaatatt ttatttagat actcaaaaaa
     2401 ggaattgttc ttttgagagg cgacagaagc accgaatgcc tctataaggc taggtggaag
     2461 tgattttggc gctttagctc ttgtcctacc cgctagttgt aaagcttgcg gcatgcatcc
     2521 gcaagaactg cgtaccagga gttttgtctt aagtaaaata tcatccgcgg aattcccatt
     2581 gcgcatgtct atcagaagcc ttagggcgga aagcacttgg cggataaaag gcatcctcac
     2641 cgtagtaagc ggaggcgaaa aaatgcggct ctcttccgag tcattgaatc cgcctatggg
     2701 cagggtcgaa ggaatgtcta tgcctcgttc tttgagcaac attccagcct cgaatgccag
     2761 aagatcgctc gacgtacata gcgcatcgaa atccttacct gggacaaggc cgcggttatc
     2821 gatcaaattg agcattgcgc ggcgaccttc gctccatgga gtatcgaggc atacaagcct
     2881 gggatcatag ggtatgctcg cttccttcag agcatcacaa taggcacggt atctttcttc
     2941 ggcggaagaa tgctgtcgag gacccgcgat aaaagcaata tgttttcttt tatggcttcg
     3001 aataaaatgc cctataaggg ctttcatacc ggaatatgca tctattccga cgaaaggccg
     3061 tccatcgatt ttaagcccaa aagtcactaa tggaacatcg atcgtatcgt taagaaagct
     3121 ctcgacggcc gattctgtcg catatccgct cagggtggaa gcccagcaca aggcagcatc
     3181 gacgttcgat gcctttgcat agcgatatac gccattcctc atgtattcaa agccgctaac
     3241 ggcatctagc cttccacccg gcagcacaaa gagaatacat cgatgagatt gagcggcggc
     3301 aatgagttct ggccatagac ctcgcgcagc accagtatgt gcagatgcaa gaagaagagc
     3361 tattctttga taatgtccct gcattcttta tcgattgtga gctcatctta tgggagttgt
     3421 caacatttta ttcttttttg cttacaaaaa gacagaaaag tccaattaac agccaacata
     3481 cgcaattgaa gactcttcaa aactcctcta taaatctcaa ttatagaaga ggaaactaaa
     3541 tgatagtgga tatcgaagaa tttggcggga tcgacgacgg atgcacagac aactcaaagg
     3601 catttgccga tgcgatggct gccttaagcg agacaggcgg cggaacgctc agaataatgg
     3661 gcagcgcata tctcacaggt ccgatagaac ttctaagcaa tatcagctta gaaattggca
     3721 agaacactac attgagcttc gtttccgaca taaaccgtta tcctccagtt ctcactcgct
     3781 gggaaggggt tgtctgctat ggcatgcacc ctcttttatt tgcaagagga gccaaaaaca
     3841 tagaactcaa aggtgaagga accatcgatg gaaatggtca gccttggtgg gaagccctga
     3901 ggcgcaaaaa gagaaaagga cagaacaaac cccttgaatc cttcgaaaca gagctggcag
     3961 agcttaataa atataatggt ggccagccct caggaggcgg gggcagagaa attcaatttt
     4021 tgcgacctcc actagtccaa tttttcgggt gttcggatgt cgctatcgaa ggcctcaagt
     4081 tccaaaactc cccattttgg acaattcatc ctgtattctc gaatcgcata agcattcgca
     4141 atatctcaat acataaccct tctgatgcgc caaatacgga tggtatcgat attgactcat
     4201 gcaaagacat cttggtaaca ggttccacaa tcgacgttgg agacgattgt cttgcagtta
     4261 aggcgggctc aggagaacag ggaattagcg aaggaaaatc ttcagagcgc attgtaattc
     4321 gcgattgtca ttttaagcaa ggacatggcg gtgttgtcat aggcagcgaa acggcaggag
     4381 ggatctatga gatccttgta agcgattgct ccttcgacgg aacagaccga ggtgttcgga
     4441 ttaagaccag gcgaggtaga ggagggacgg tcagcaggct tagattctcc gacctgcata
     4501 tgaaacgagc actatgccca gttgctataa acatgtatta tcgctgcgga gctaaactag
     4561 aggaatgctc gaagctcttt tcgctggaac accagcctgt tgatgctctt actccccaca
     4621 tccatgacat cgaaataagc aacctaagag cccaagaatg ccgcgcctcc gctggattca
     4681 ttattggctt gccagaatcg ccaatagaga acctacgcat gcgcaactgt aggatcgagc
     4741 tcagcagaaa tgatatggtt gctccatcag aatctgaaat gttcgaaggc ttgcctgaaa
     4801 tgcaagcgcg cggaattaga ataaggaatg caaactgtga tttcgatggg ctcgaggttc
     4861 ttggccttcc agagggagaa gctccctatt tccgcgaacc ctcacctaat tagaagagcc
     4921 cgggatttca catccatttt ttcttcttga aatagatcaa ctccgctatt accactgcgg
     4981 ccataagcaa tagagcagcg ggatatcccc agggtttgtt caattcgggc atataggcaa
     5041 agttcattcc atagatacct gctatgaaag ttatcggaat gaaaatagcc gacaatattg
     5101 tgagtagtgt cattactcgc gacatatttg tatttacctg gctcagatgc aattctatca
     5161 ttgaagtggc atgctctcgg tatgactcga gcgcctcaat tgcctgcact gtattctcat
     5221 aaagatcacg caaataagga gatagagaga catccatctg gatggattcc gtacgagaaa
     5281 gcgcggcgat agaatcgcga acaggccaga tgattcgacg catccttgaa aattcccgct
     5341 ttaattgctg aatgccatga aaaaactcct ggtcgctttc ggaatcggac gcctcctcga
     5401 atcgctcgag catatctcct aacatttcta gcacaataaa gtaattatcg acaatcgagt
     5461 ccaatagcgc gtacagcaga taatccgcgc catgctttcg cagcctccct gctccagaca
     5521 ttatccggtc gcgcacagcc ttaaagcaat cgcccggcct ttcctgaaag gaaatcagca
     5581 atttgccttt gaggataaaa ctgacctggt cgtattcaac cgagccgtct tcgtgagctg
     5641 ttatcatctt tgttatcaga aaaagataat catcatagtt ttcgagcttt ggcctatgtt
     5701 ctgtattcaa aatatcttcg attaccagtg ggtgaatgtg cagcatgcca cagagctttt
     5761 caacgattcc tcccgaaagc ccgttgatgt tgatccaatt caccacatct agatcgatga
     5821 gagccagaag ctcgcttata tcatccggaa tactcattac agcgcttgtc tcatcgtagg
     5881 aatacaatga ataactcgct aatgtaggct ctatatcgcc aatgtagtgt ggagtgcctg
     5941 gtggaagccc agcagtttcc accgagacac cgctaaatgg cagcttggaa gcatggtgct
     6001 taggttttgc agcttttgct ttttcgctgt ttgattgcgc actttcgcgt gaggcctttt
     6061 tttcgaacac actctgctca aaagccacac cttcaccaac ttcggaagaa gctctgcata
     6121 ctgtgttggg ttcggcacta ttctgcttct tggaaaacat cttcatatct tatagactag
     6181 ctattcgggg gcatggaagc aagcccggaa aaaccctgac tggatatggt cttcatatcg
     6241 ctcatctaag ctaagctttc ttcatcgata cttgttttgc cgatcctgca aattatgtcc
     6301 tttgtgcgct gctatttgtc attgttttat cactcaagaa cagtactatg cttgctttga
     6361 tcgaccggag aaaatcacat cccattgtgt cgctagcatt ccaagcagca taaggaccga
     6421 cccaagcatt gtgcgcaagc tcggactttc tgataaaaga agtatacccc caagggtagc
     6481 gaatactcct tccagacaca ttatgatcga tgaatgagct ggcggtgcat ctctttgagc
     6541 caatacctga agagtataac ctactcctac agatcccaac ccaccatata gaataggaac
     6601 cagcgcatct aagacggcat taggcgatat tttctcaaaa atcattgcag ctgcgaaaga
     6661 aaaaaccgag caccaggcaa actggccggc ggccagctcg actggatcca atcgcttggc
     6721 caagttgtct ataaccaata catgaaatgt ccagaaaaaa gcgcttatca gaacgaaaat
     6781 atcacccgag ttgactcttc caagcttatc tggtgcgctc agagtataaa tacctactag
     6841 tgcaagaata gccccaaccc aggtgggcaa acctgtagat cttccccaga ccattcccac
     6901 gattgggacc agcaccacat atagtccagt cagaaacccc gcttttcctg ccgttgtgta
     6961 ttgaatgcct atctgctgca aagaagcagc aataaaaaga aagctccccg ccacaaaaga
     7021 accagacaag aagagctttt gcttgctagt tgcgcgagtt tggtcttcat tcagcatgct
     7081 cctctgctcg cacccggctt ccccagacct gtccaattcc tcagaactat ccaagcctct
     7141 cgatccattt ttgcgattac gtttgcgggc atacattatc aatgggagca acgaaatgct
     7201 ccccaatgca aagcgaatcg cattgaaagt aaaaggcccc acataattca ttcccactcg
     7261 ctgggcaaca aatgcaaaac cccagataat tgccgtcagc aaaagcaata aatcagctcg
     7321 aattgcggtc ttattcatgg tattggattc ctctaagcag aactagctcg actccagggc
     7381 taattcccta tgccctttca tatactctct ttgagaaaaa gaagaaagac cgctgatgtc
     7441 caagcgaaag ctggatcgtc caatccctca ccatggatag ggtcgaagtt ctcagccatt
     7501 cccttctgtg cacatagatc gacaaaccgt tgcgcaattc tattcgccaa gttcaattct
     7561 cctagcagcc taagtgcatc gaatacaagg taattcaccg gcgcccatat cgggcctctc
     7621 cagtatccat gaggctccat aaaagaagaa tccaatgatt cggtagccaa gccaaaaggt
     7681 gtcaaaaatc tactttcatc cgaaatatct gaaatgagct ttttgattac ttcttctggc
     7741 aagcgcttgt tcgcaactat cggcaagcga agaagaaaag agtctctatg ttctattctt
     7801 tctcccaaga ggccttttcg tgcaaagaac ccattatcgt cccaaaggcg attcagcatc
     7861 gattcagcca acgattctgc taaaaatgtc cagttctgcg cttcttcgat accatgctcc
     7921 agaatacgcg atatctcggc aagagaatca cactgtcgtg agagatacgc gtatagatca
     7981 ggagattcga caggtcctcc atgcataaac agcgttgcat tgtcccagcc agaatcattg
     8041 ccatgtcgat agataatggc tccagaaggc aatctgcgaa atctgagcca gaaattggtc
     8101 gattcggaaa tcgacttgta aagatattca atgctttcgc tggaagactg aagtatggtt
     8161 ggatttctct ttatcatttc aagcaaagtc catccatgaa tgggaggctt gcaaaagctg
     8221 aataacgaga agcgatcgtt gatgcaatca ggataggcac cagctttatg ctggttatca
     8281 gcgaacacca ataattggtc taaagcaagg tcgggcatgc tttccaccat cgcaagcgcg
     8341 caaaaacagt tgtcccagct ccagatattc gtcatccaat tcttggagct atatacagag
     8401 ttgcgcgtga gctttccctc aggcctcaca aaagcggacc aaaggatata tgatgccaga
     8461 tctctagcct tacaatatcg agattcaagc cctgtgtagg tataattctg attccattgg
     8521 gtaaagtcga aaaatttggt gtttttggtg tccgattcac aaggaaccaa ggcatactct
     8581 cgaatactgc ctactccttt gataatatcg atccgaatat attcattgcc ggcccttccc
     8641 catggggcct ctacattata aactgcatct ctcaagtcga gttcgtagac atgatctttt
     8701 gcgcaactct gaattatgaa tatcccctta gccttctcct gaatgaaatc atggcttgat
     8761 ttatgcattt ccagcctaag acgtatatca cagcattcaa tacttattac atcggaaaac
     8821 aggaatttca gaagaacata gcgatcaggc gaattcttag cgcgaatccg gcattcgttc
     8881 tcatttgcct ctatcacttg ttcgagttct tctttttgat cgagcccttc tttgcccaag
     8941 agaagcttca tcgagaatag ctcactcgga atctcgtctc cccctccgat atccttcaaa
     9001 tagaccgaat catcttttcc gatcgcaaca gagaaacgcg caccaaaagc cgaccaaggc
     9061 gttttcgaaa tatcaagatc cattttttca agattctcca atagattttg catttgccag
     9121 aaacgaagtt tccggatcga gtgcgtagaa ttcaaggcct agatcgaatt cctgaggctt
     9181 caatctatat ggaggaagct gatcttgacc acatgaattg ctgccgatac cattttgcgc
     9241 aaaatctaga ttcagaatac aataagcagt atgagcaatt tcatgatcat aacacgcttt
     9301 ttccaatgca tctacatcat aatcatgata agagaattcg attggctttt cggaaattat
     9361 cgctaatcca aaaccttgtc tatctgtaat cgaaattgca tcagtgccat atctattgcc
     9421 attctcctgc ggtcttatat atgggattgt attcaggatc gttcacaagc cttgtgctct
     9481 catcgatcca ctcgaatcca tgggtctcga gatcggtttc gtctatgaca taaaggccaa
     9541 aataatcgca caactcgaga aaatagggat gattgggata gtgcgaagta cggacagcgt
     9601 tgatattatg ctgcttcatc agaagaacat cttgaagcat cgtccgcctg gtgacatatc
     9661 ttcggttttg ttttctatct gcaattcgac ctttagaagt gcattcttat atatatcatc
     9721 gaaggatacc tcatgtgaca catcgaacaa cgaaacaagt gggacttcgt atatctcgac
     9781 atctctgaat attcctgaga gccaccacat atcctgatct tccagatagc ttccatcaga
     9841 ccactgatat actcttacca caattacgtt tttgccaact ctaaccaaag aagtgatatc
     9901 gaattccgac aacaggcgcg aacctttaga aaaccctgcc agctttccat ttatccaaac
     9961 atggaatgca gaatccacac catgaaatct caaaatctga cgacgcgaca gatcatccaa
    10021 aaaaaactga cgccgatata ttccggttgg atttgctctc gggacaaaag gcggcctcac
    10081 gggaaatgga taatataggt ctgtataatg tggccttcca tgacctttta gctgccaatg
    10141 cgatggcacc tcgatatctt cccaagcgcc gtcatcgaaa tcaggctttt cgaatccaaa
    10201 aggagaaagc tcaggagcct caagaaaaag gaatttccat gatccattca agttccgaaa
    10261 ccccagttgt tctgattcat cgtctcgtaa ggctccttgc acatctttcc atcgttttat
    10321 gtatgctcgt ccaggtagcc gatctatatg gagaagcttg tgattctgcc agtcgcattc
    10381 tgcaccatat gatgtgttca tgctctatcc tttcagtgct ccactcatgc cttctattat
    10441 gtgcttctgt gcaaatgcaa atgcgatgat aggtggtatt atcataatcg cggttatagc
    10501 cataatcgga ataacctgca tttcgtgcat gccctaaaaa tggcggcggc aagacgagca
    10561 atacgaatag atgacattta ctatagtaat agaaatgacc ttgcattgct ctcttttgat
    10621 agatcggcaa cgatcattat cggcagcgag ggtggctgcg atatatcatt gctaaatttt
    10681 ctaaagaaag aaaagaacgc tgtatgcatc gacttcgatc ctgatttgaa aaacatagat
    10741 gttgtatgtg cagatttcgg aaaatctatc ggaacaatcg caaagagatt tgctgagctg
    10801 ggtatttcgc gtattggtta catcggaggc aaagaaatct ctcctctcaa ggggaaagaa
    10861 ataatcgacc ctagaagcgc acaatatata gaggaatttg ggaaactggg tatctataga
    10921 aaggaaatct ttagagctta cgggccatat acccctgaaa atgcgtatca attaactaag
    10981 gagcttttat ccggcacaac caaaccagag gctatatttg ttgccagcga taatatggct
    11041 ctaggtgtct ataaggcaat ttgtgaagcg aatctttcga ttccgcagga tataaaagtt
    11101 atcagctgca atgatcaacc cgtatcggca tatatgacac ctccattgag cacaataaga
    11161 attccgaggg cagtaatggg ctcagttgcg ctatctctgg catcatcgcg agctgcggat
    11221 tttcgtgagc tcggggttaa agtaatggtg cctacagacg tggtgtttcg caagagtttt
    11281 atctagtgga gtattaagat cgagcccata aataattttt tatttttcgc gtggatcgaa
    11341 aaatgcggaa gctctcatgc catatagaca ccaccgttga tatctaatgt ggcgccggta
    11401 atgaatccat cgtattcact ggcaagaaaa gccaccgcac gagcgacatc ctcgcatgta
    11461 ccagccctct ttaaaggtat atctcgtatc gttgcctgct tggattcttc gcttgtgtga
    11521 atggcatgaa aagccgaacc aagaataaaa ccaggtgcca cagcatttac cctaattcca
    11581 tagggtgcaa gctcaacgga gagcgcacgt gtaagggtaa gaatggctcc ttttgtcata
    11641 gaatagacta tcgaaccacc atggccaccc ttacgtccag ccagcgacgc aaggttcact
    11701 attgaggcgc agcccgacct tttcaataaa ggtatcatgg ctcgcgtgac caagacattt
    11761 gagtctagat ttactgccac gacttttcgg taaaaatcca tccgaacatc ttcgagtcta
    11821 tagcgtccaa ccaggtcacc tgcattgttg ataagcacat cgagattatt ccatctctca
    11881 aatatgaaat ccgctaggat ctgcacacct ttttcttccg aaagatccgc ttgcaccaca
    11941 tctgccttgc cacctgaagt gcgtatttgc tctgctaggc tttcggccaa ctctctgctc
    12001 ttgtgataat gaataatgag atcagccccc atattgctta attccagaga aatagcgcga
    12061 cctataccag atgctcctcc agttaccaga gcttttttcc ctttcagact gttcataagt
    12121 gactccataa gatgaatata gccaaagctc attgctagta tagatgcgag ctagagtcca
    12181 cgcaagttga atgatcttaa gatgacaggc aatcgaactg ggtcggctgt gctcgccgca
    12241 tgactctctg taaatggatc gggattcaat aatggattcc gcttattttt tatcgagatt
    12301 ctcttgaatc acacttgaca agttcaaaag atgatttatc atatgaaatt ggattccata
    12361 ttgtggaatt ctatagatga atctcatggt ttgcggcatt ctgagaatgt attccagact
    12421 atgggatatt gtttggcctt agtcgcctta agaccgacct aagggctgaa taagcataaa
    12481 gatcaattat agggggtttg ttatgaagaa aaaactaatc gctctggcag cgcttttgct
    12541 cattacggca agtttcgcta ttgccgctcc aaaatacaac tggaagcttg cgtcagtact
    12601 gcccgactca catccggttc atcaggctct ggttttcttt gctgacgaat tagcacgaaa
    12661 gagcaatggt gatatcaaag tcactctctt tcctgccggc cagttgggtc aagaagcgga
    12721 ttacattcag gctataaaga ttggatcgtt ggaattttcc aaggtctcat caggcccact
    12781 tggacaatat gccccaacac ttcaggttgt ctctctgcct ctaatctgga gagacctgga
    12841 tcatcagcat aaagtgttgc aaggacaagt tggcaagaga ttgatgaatg atctcgataa
    12901 ggcaggcttc aaggggctag tctttatgga tgcaggtttt agatctatta ctgcaaagaa
    12961 gcccatctac acccctgccg atctcaaagg cctaaagatt cgcgtcatgc agtccaagcc
    13021 ccttatcgat acaataaacg cattgggtgc aacagccgtt ccaatgggtc aatctgaagt
    13081 ctacagcgcg ctgcagacaa atgtcattga tggctgggaa aacaatgagc ccacagtgct
    13141 atccttcaac atgcaggaag ttgctaaata tttctcttat acgcgccatt cctctattcc
    13201 cgatatcctc ataatgaata aaaaactctt cgatggtcta cccaagaaca tccagactat
    13261 gatcgaagag acagccttcg aagcaatgca aaagcacaat cagctctggg gcgaaatgat
    13321 cgataagaat gtcgctgaac taaaagctaa aggcatgatc ttcaatgaag tcaaggacat
    13381 caccgaattc caaaaagcat gcgccggagt gtggaaggag tttgagccga tcgtaggaaa
    13441 agatttgata gacgctatag tcaatacaaa atagatggcg ctgttgctta gatgctaaat
    13501 acttcctgtc tatcatgaca ggaaaaaaat gggcggcaga tattctaccg cccattttca
    13561 aaactagaaa ggattgttct aatgcaggat catcaggaag gcgatatgcg aaaagacctt
    13621 tggcacattg tcgatagatt agaacggata ttaactggat ttattgccat tttaatgggg
    13681 atagtgacca ttgtggtgtg ctggcaggtc ttcgatcgtt atgtattgca taaaagtccc
    13741 tattgggctg aggaattctc ggtaaccata atgatgtgga tggggctcct cggtgccgcc
    13801 tgcgcagtat ggactggcga ccacatgaga cttgaattgc tcaccaaaca cctgcctgct
    13861 cgttttggat tgattttaga gatattgatc gatggagcag tgatctattt ctcctatttc
    13921 ttattcaagc aaggtattga acttgtaaac gcaatgtgga cgagccaatt atccaccatt
    13981 cgactttcta ttggtattac atacctggtc atcccgatct cggctgccat gatgattctc
    14041 ttttctattc tcaagattgt aaagaaggca agcggagcaa atgcaatatc tagccaacaa
    14101 gaaaccaaaa acggaggcga agatgctcgc tgaatctgtg ctgttgggat cctttataat
    14161 ccttatcttg cttggtgtac caatttcttt cggtttgggc atttcatccc tgctttctgc
    14221 atttgccgca ggtctgcagc cggctattat agctcagcgc gtagccgcag gcatgcaatc
    14281 gtttcccctt cttgcgattc ctctttttat cctggccgga gcattgatgg ccaacggagg
    14341 agtagccaag cgaatggtgg atttggccta tgtaatcgta ggccctttgc ctggtggtct
    14401 tgccatagtc aactgtatcg attcgatgtt tttcggtgga gtttctggat cggcagtggc
    14461 cgatgtatcc tcaaccggac caatgataat accgatgatg gtggaaaaag gctatgataa
    14521 ggacttcgcg gtagcgctta cagtggcttc ggcaagccag ggcataatca ttccaccttc
    14581 ccataatatg gtgatatatg caatggtagc aggtggagtc tctgttggga gactcttctt
    14641 agccggttat attccaggaa tgctgttggg cattgcgctt gccattgcat gctattttat
    14701 tgcgctaaag cgaaattatc catgcgaaaa acgacctccc cttaaagaga gcctgcgcat
    14761 catcttagat ggaattctcg ccctcctctg cgctgctatt ataatagtgg gaattgcggc
    14821 aggcattttt actgcaaccg aagcatctgc ggtagcagtt ctgtatgcgg ctttcttagg
    14881 cttctttatc ttcaaggaac tcaagctgaa ggatttgtgg ccaattttca tggattcggt
    14941 caaaacaacc tctgttgttt ttttcctcat aggttgcgct tcggcctttg gttggatgat
    15001 gacctaccta cagatacctc ttgctgtaat gaatttcttc ctgagtctga caaataatcg
    15061 cttcctaata cttctgctcg tcgatgtcct tcttcttttg ttaggcatgg tgatggacgt
    15121 ggcgccactt atcgtaattg tgacgcccgt attgctgcct gttgtcaagg cagctggaat
    15181 gagcgagata acctttggca tagtgctcat gctcaacctg ggtatcgggc ttaccacgcc
    15241 tccggtcgga gcaagtctct ttacaggctg catgattggc aaaacaacaa tcgaaaaagc
    15301 aagcaaggca atgcttccac tctggccagc gatgcttgca gtgttatttc ttgtgactta
    15361 tattccttgg tttactgagt tcttgcctaa tcttttttac tgatacgaac cttgtgtatg
    15421 ggttaggctg atagtaagaa atctcctcac ttatagctaa atacaactaa ggaagatcaa
    15481 tattatttcc ttcgacttat ttatttctta tttgctctct ttgccgcatt tctgctttat
    15541 tatcttacac aaggttcgta gcactaatca aggatttagc tatactctat agtgcaccct
    15601 tcaaaggagg atcaatgctg aaaccacatc taatctcgac tctatccggg gctagctatc
    15661 ctttccaaca gctgaatgaa tttgccgaaa atggcgaatc gctcgaagtg gttatcccta
    15721 atattgaaca tgctcggcca ctctcgacat cgactggtat atgtgaccgt atttgtgcta
    15781 gctcaaaacg caaagcgggc acgctaggag agcctatggc tcgaggcaca ttgattcgca
    15841 cccagaatga aaatgcagcc gcttctgacg cctcaatctg gcagcgttac gccgattttc
    15901 tgccttttca agaaacctat cctgagctat ccctcggcga aggcatgaca ccgcttgtct
    15961 cggcaggtaa cgcactttca acatggacgg ggctggataa gcttcttctc aagaacgaaa
    16021 ccgtaaatcc aacatggagc ttcaaggatc gaggctcgct tgcttctgct attatggcta
    16081 tagaacagaa agagcgagta atgatgacca tttcgacagg aaatatggga cagtccacgg
    16141 cagcctacgg cacacgagct ggcctaaagg taattgtatt cttgcctgaa tttgtaccgc
    16201 aagaaaaaac cctggccatg gcgattcatg gggccactgt tttccgtgtt catggcacaa
    16261 attatagtga tatgaaagag aaagtcctta tgcttgcttc tagacatggt atccgcgtaa
    16321 catcgggcaa taaccccata cgtgttgaag gctacaagac tgaagccttt gagcttttcg
    16381 aacaactagg cggagaagtg ccagactaca tcgctgtacc taccagtgcc tgcggacata
    16441 tccgaggaat cttcaaaggc ttccgcgagc tgcacgcagc aggccttatc gatcgactcc
    16501 caaagatgat agttgttcag gcggctgcta acagcccaat cgcgagcgca ataaaatcgg
    16561 ggagtctcac catgatccca tttgccaatt tcactacggt cgcagaagcc ctatcttcag
    16621 gcaaaccacc tggcggcgat gagataattg caaaagccta ccaatatggc tggcttaccg
    16681 aagacgctac cgaagaagaa attatcgaag ctcaaaagcg actcgcgggc gctggcttct
    16741 ttgtggaacc agcaagcgct acgactcttg ctgctctgaa gaaactgcgc gcctcgggga
    16801 caatcgatgc gaaatcatgt gtggtcgcgg tgctgactgg ttcagggctc aaggatacta
    16861 gcgtcctatc caggcataag ctcgatatcg tcgatacaga tatgcatggt atagagaaat
    16921 atatttcagc tgaaagatct tgaagtcaat aacagataag atttcctgat cgaagactac
    16981 gcagcagccg cagagaacat actgctcgct gctcagggga taggactagg cggcgtatgg
    17041 tacggcgttc tgcagcgatc ttccttgaac gagtatctta gcttggtttt gcatcttttg
    17101 taattatatc tcttacggca tgacttaatc atactgtgga catgtctgtg gcaactgcat
    17161 cttgttggat tccctaaaag gatgcatggc tcctatcgga acgcacaaat cagaagattc
    17221 ttccttacat ctatgccatt agaaacaatt caatcctgaa agattcttag aactataaaa
    17281 aaacagcctg gtaccaaaac cctcaaatgg cttttcagga gttgctattg catatgctat
    17341 cgctcccaaa taaaagagaa tatcaggtca tcttctggca acttcactct aaaggcttcc
    17401 ctgcaaggct caaatccaca tggcagtacc cgccaggatt tttcagtagg taatcctggt
    17461 ggtattcttc ggctgggtaa aagggcatag cagccacaag ttcggtaact atgggctttt
    17521 gatatatcat gtttgcagag agctcggcaa tgattttttc agcaatgacg cgctgctcgt
    17581 cgtcctgata gaagattaca gatcgatatt gagtgccgaa atcagcgccc tgtcggttga
    17641 gctgggtcgg atcgtgcatg cgaaaaaagt ggcgcaggag ttggtcatag cttatcttgg
    17701 cggggtcgaa ctcgatacgg agcgattcgg catggttggt ggtaccactg cagacttgct
    17761 cgtaattagg atttggggtt gtgcctccgc agtaccccac ggtagtcgag agtacgccag
    17821 gcaaacggcg gaaatactct tcagtacccc agaagcaacc tgcagcaaag atggcgattt
    17881 gtattgagtc agtcttggct ttcataggcg tatcctgtgg gttaaataaa gacgccaatg
    17941 cgctgctcct tatacatatt tttgccttaa aatggctccc tcatttgtct tttaagacat
    18001 tccgttcatt cctaattatc ctgctatttg tccaaaatcc cgtccatcct taattatctt
    18061 gtcatccagt ctaaaatccc atctatctta ttgaaatcaa aaaatgtatt ttcattgtat
    18121 taaaataatc caaaataatg ctttgcattt agagtaatct attctttata tcatctcata
    18181 aagctctaag ataatagctc caactgtcat ggttgaaaga tcagtttcta tttcaaatgc
    18241 tcttcgagcc aatcctttac atcctgatga acctcgtcgc ggttgatctc gttgagcacc
    18301 tcatgccttg ctcctggata taggatcatc tttacatctt cgataccaag ttccttgtat
    18361 ctctgaacca
//
LOCUS       DAOMZL010000031        18210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_2716_length_18210_cov_17.822253, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000031 DAOMZL010000000
VERSION     DAOMZL010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 18210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18210
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2716_length_18210_cov_17.822253"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..2225)
                     /locus_tag="PLQ30_04085"
     CDS             complement(117..2225)
                     /locus_tag="PLQ30_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPW01655.1"
                     /translation="MKTILDRNRRHMPSRLLSLILICLSTLNLQAQQSQPFSGELPSF
                     DNFEFAFPEGDALRNITKPVFLGEKTFIARLEQRTEFPDFDKNQNSEPRPPLGIVLCG
                     GSARAYAHIGVLKILEKANIYPDFIVASSMGAVVGMLYAAGYSPQDIEFIIKSLPLES
                     FFTPVIPTNGGLINTDMFKAILRKIIGRLDVSETAIPIIIIAEDLKSRQQIWIAEGPF
                     DLVMASAFAMPAIFEPQEFDNFVLIDAGATTIAPVEPALIFSDRLVISTAFYNRAMDF
                     SSPVTVINRAVDIGKTRSGMAGIERSKALVIRNDVENLSYMQFTDPDIIIEKGELSAQ
                     NALEQLDLISRKDLENPPSPEFFELRSEMHENLNNTIQDLQSGQQPSTSLMLRGIPIL
                     RLFDPFISMPGETGTEARIGASLAFSYLKFKSYLSYFNALNPDPGKVWAIETAFRVNP
                     LDSLNAWFTARLWGDYSSTSILSHDSSCWELASRMIWMKAQEERKRGFHIGGDIFFKM
                     DFSIANWQTCAFFQTEMPRKYEKSGPSIQPWYSAYIGAFAENSVGLKNAVGLQGSLQG
                     GFDSKWVSPKFRAFSKVSLNNQEFMESKYDGFRSIATRKAVNSNLITNVEIPFSPHEL
                     FLDISEALLLKGFELAPFFDTRWNSTSDENFHMNDWAAGLSFSFEARAFGLAPATMTF
                     YASYSGSKIISLQFRAGMLL"
     gene            2530..3828
                     /locus_tag="PLQ30_04090"
     CDS             2530..3828
                     /locus_tag="PLQ30_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPW01656.1"
                     /translation="MISEILVLLALIFINGFFSLSETALVSSRKARLKAEADKGKSTY
                     KLALNTRESPSRYLSTIQVAITLIGILTGTISGTTFSQLLAQWLSGWSFVGRYAESVA
                     VAIVVLTITFLSVVIGELVPKSIALRNPEYIAALTIKPMHALSVVFYPVVRFLSSLTD
                     SLTKLFRFSHGAEPAITPEEVRILLEQGEKSGVFETAEREMMEEVLNLDDRRVTMFMT
                     PRTEVVTLNIKDSRDSQIRAIIKNSEYAYLPVVDGDLDHTIGMVAVKRALTCMAEGCF
                     LSLETCMEPAVMIPESLSGLQALAILRDAKRSAGLIVDEFGGISGIVTIADLLEAIAN
                     LSEVETEEASQIIKRSDGSYLVDGSMPIDEFAESIGINLDVISNEDYDTVAGFILHCG
                     GSIPKAGDIIEWPPLKMEIVDMDGKRIDKVLVQKSEQSLT"
     gene            complement(3892..4335)
                     /locus_tag="PLQ30_04095"
     CDS             complement(3892..4335)
                     /locus_tag="PLQ30_04095"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPW01657.1"
                     /translation="MPKIIKNRQKALAWLRSALTFSKAQISAFVGGVIDYCTMIFITE
                     VFHIHYTISIAAGGMVGAAVNFSVNRTWSFYSKDAQYEFSVIQQLVMFFPVVLGSIAL
                     KSAGTFAFTTLSKIDYKITRLAVDVVVSIFYNFMLQKHWIFKKRH"
     gene            complement(4313..5059)
                     /locus_tag="PLQ30_04100"
     CDS             complement(4313..5059)
                     /locus_tag="PLQ30_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01658.1"
                     /translation="MNKDHGRTKIKQKKNSIDHALPLISKDRVRTNLLKRQEQKALAF
                     LVQYIPSWMSSNVLTGIGFLGNVIVFLSFILAELFDTRFLLIGVIGFAISWFGDSLDG
                     RIAYYRKKPRKWYGFMLDLVIDWLGIVLIGLGFVIYADRFLKLLGYAFIVLYGLEIII
                     ALLRYKISGSYSIDAGKLSPTETRILISLFLVIEVIFPGSIVYLTAIADIILAISNIS
                     EFKKLAKIANERDEEENRKSELKIAENNKE"
     gene            5209..5895
                     /locus_tag="PLQ30_04105"
     CDS             5209..5895
                     /locus_tag="PLQ30_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01659.1"
                     /translation="MDKKRFFYVAIYAIGMAYLEASIVVYLRKLYGISDLMISVPAFD
                     PMVSIIELGRELATLIMLLAVGLVSGKKFQSQIGFSLYAFGLWDIWYYIWLVIFIGFP
                     QSLFDWDLLFLIPLPWWGPVIAPVMISVLMIISGVRLALLEEKGLLVRLKWLDWAMLA
                     LGSGTMLYAFMSEAISSLPASVENLSDFRPSSFLWPIFLIGYAISIYATWRILYKSLN
                     EPAMHRSVRK"
     gene            complement(5845..6429)
                     /gene="pdxT"
                     /locus_tag="PLQ30_04110"
     CDS             complement(5845..6429)
                     /gene="pdxT"
                     /locus_tag="PLQ30_04110"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HPW01660.1"
                     /translation="MSSHRIGLLALQGDYEAHGKALVRAGFPRCDMVEIRSFSDLQSL
                     TAIIIPGGESTVMSMLLERFGMFEELKRQIENGMPVLATCAGLILLAKNIEGNDQMGL
                     GVLDIKVKRNAYGRQVDSFHAPVQTIIDSVGIIEGIFIRAPRILASGKEVEHLAIYEN
                     EPVMVRQGSIIAATFHPELLPDAPVHRWFIQTFV"
     gene            complement(6426..7331)
                     /gene="pdxS"
                     /locus_tag="PLQ30_04115"
     CDS             complement(6426..7331)
                     /gene="pdxS"
                     /locus_tag="PLQ30_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HPW01661.1"
                     /translation="MEQKLWSDRQSEQWHIKVGLAEMLKGGVIMDVTNAAQAKLAEKA
                     GACAVMALERVPADIRAQGGVARMSDPKIIKEIQDAVSIPVMAKCRIGHFVEAQILEA
                     LGIDFIDESEVLTPADDRYHVYKHPFKVPFVCGCRDLGEALRRIGEGAAMIRTKGEAG
                     TGDIIEAVRHMRTVMDGIARLLGLPEEELMTEAKELGAPFELVWEVAKTGKLPVPNFS
                     AGGVATPADAALMMQLGAESVFVGSGIFKSSNPERRAKAIVSAVVNYKDPKILAEISE
                     DLGEPMVGIGLDELIENQKISGRGW"
     gene            complement(7536..8954)
                     /locus_tag="PLQ30_04120"
     CDS             complement(7536..8954)
                     /locus_tag="PLQ30_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HPW01662.1"
                     /translation="MKYNPISRDIIRELINIAGERNVLLDPAQLEIFSHDETSKEEYG
                     HMPEVVVTPRSTSAVAEIVKLANRTSVPITPRGAGSGLSGGAIPIFGGIVLSLEKMNQ
                     VREIDYDNLTMTVETGIVTNEINNLVKEKGLFYAGYPMSLETCTLGGNIAENAGGGKA
                     IKYGVTSRYILGLEFVTPTGEVVWLGGKLAKDVTGYDLVHLIVGSEGTLGIATAAIIR
                     LIGLPAAKSDLLVLYRSPQEAISSVPKILSKGLIPTSIEFMDRRSVETSCTYLNENLP
                     YAQCGAMLLIEMDGRDQSLVESEAEAVGDLCMEQGAIEVYVADNRTTQERLWAIRRNI
                     AEALKVYCPVQSLEDIVVPPTAISAVMPELERIALQFGIAIPCYGHAGDGNLHATLVK
                     DPQMPMEQWQQIEQQALLALYESITALGGKISGEHGIGLKRKHFMRKFMSPVELNLIK
                     AIKRAWDPNLIMNPGKIFDIED"
     gene            complement(8944..11085)
                     /locus_tag="PLQ30_04125"
     CDS             complement(8944..11085)
                     /locus_tag="PLQ30_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPW01663.1"
                     /translation="MHIIVLIKQVPETDKVKMDPDTGTMVRSGLESIINPLDLYAIEE
                     ALILKERFGAHVTTISMGPLDAERSLRESLAMGCDEAILLTDKAFAGSDTWATSKVLA
                     EAIRHIDNADLILAGERATDGDTGQVGPAVASWLEIPVLTYVSSLEIESKTEDLETAE
                     SNRNRTCHPTFLVRRLTEEGYQSVRVQSPCLITVVKEIASPRLPTLRGKKFSRSAEIL
                     HWGAKEINLDPASLGLAGSPTRVVKIFYPKVSRDGERIHALDEPGIEKAIERILSMIR
                     PVIGSPIMGDRGSRESRRGYTETSPAVDIEESAIGVLIAKMQPALQESVISLSEKVFS
                     EPATDPKPEFWIIAERRKRGLDIVSFELLARARPLADSRGAILAAIVLGSASPDEAAM
                     LIAHGADSVILVENPSLEDFVCELWSETLLKLAYARKPEVILGAATTTGRTLMPYLSA
                     KLGTGLTADCTELSIEENSGLLLQTRPAIGGNIMATIKTAHHKPQMATVRPHSMSPLP
                     PDFGRKGIVIHVIDESSKERIISDFSDSRVQVLALESNVKDFENLESASIVVAGGRGL
                     KKADNFKLIRELASALGGAVGASRESVDRGWTSYPHQVGLSGKTISPEIYICAGISGA
                     VQHLAGIRTAKKIISINTDPDAPIHAVADLAIIGDLFEILPRLTARLKPIYSSSYPSL
                     SSSPYQTKDTRSQEPRILDSKTLSTEAQHEI"
     gene            11382..11600
                     /locus_tag="PLQ30_04130"
     CDS             11382..11600
                     /locus_tag="PLQ30_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HPW01664.1"
                     /translation="MTKNMGKTDKRVRIIVAVVIAILILTGVLKGVLAWILGIIAAFF
                     VVTAAISFCPLYTLSGCSTIHKEKTEKK"
     gene            complement(11686..12024)
                     /locus_tag="PLQ30_04135"
     CDS             complement(11686..12024)
                     /locus_tag="PLQ30_04135"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HPW01665.1"
                     /translation="MRVFYIVLLIAVAVLAVIFAVQNSAPITVSFFGWSAQASMSIVL
                     VLTFAAGILIGMLLLFPSIWKRMRALSAQKRKTSEARKLAKETQETKASPSPEAGQEE
                     SLSASHDTLN"
     gene            complement(12066..13925)
                     /locus_tag="PLQ30_04140"
     CDS             complement(12066..13925)
                     /locus_tag="PLQ30_04140"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HPW01666.1"
                     /translation="MKTLKMSPSYVKNERLLSWVSSMEELCKPDLVYWCDGSDEEYDT
                     ICRSLVASGTFIALNPEKRPNSFLSRSDPSDVARVEDRTFICSKDKADAGPTNNWVEP
                     NEMRGILNKIFAGCMRGRTMYIIPFSMGPIGSPFSYIGMEITDSAYVVANMRIMTRMG
                     RKVIEALGENGEFVPCMHSVGMPLEPGQKDVAWPCNKQTKYIVHFPETREIWSYGSGY
                     GGNALLGKKCFALRIASVIARDQGWLAEHMLILGLESPSHEKTYVAAAFPSACGKTNF
                     AMLIPPESFEGWKVTTVGDDIAWVRPGKDGQMRAINPESGFFGVAPGTSEKSNPNAMA
                     TIRANTIFTNCALTPDGDIWWEGMTKNPPEGLIDWQGRPWDPASGKPAAHPNARFTAP
                     IKQCPSIDSAWDSHEGVPIKAFIFGGRRSTVYPLVYQSFNWIYGVYLAATLGSETTAA
                     ATSAIGLVRRDPFAMLPFCGYNMASYFDYWLHVGLYNPNPPLIFGVNWFRKDADGNFI
                     WPGFGENIRVLKWIVDRANHKANAIESPIGWMPRYEDLNWEGLDFPKERFIELMSVDR
                     ELWENEILSHEELFVKLYERLPKELIFVRQLLLSGLWRAPEHWKSDTIDSTIN"
     gene            14002..14508
                     /locus_tag="PLQ30_04145"
     CDS             14002..14508
                     /locus_tag="PLQ30_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HPW01667.1"
                     /translation="MPNLSSPEHRLFFCMNIIYAKRMNSLSVQTQYWIMAISIIVFAI
                     TIITAVLAYKAGKKHGFLDAQVSENERMEHARKDAIERSRAVLSGQISEQLAPWLPDF
                     PVNPSDARFIGKPIDFVAFCGTDEGSVSEIVFVEVKSGKSALSYVERSVRDAVVGGHV
                     RWVEYRIE"
     gene            14720..15067
                     /locus_tag="PLQ30_04150"
     CDS             14720..15067
                     /locus_tag="PLQ30_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01668.1"
                     /translation="MTTKDVPDIISAEVLQGDFEDRAVKIVYTSDLLSDVLANGRDAS
                     VLVTIQAHKNTVAVASVVDIPLIVLCNNRPIIDDMIETAAQSRIGLVRTALSQFEVSG
                     RLWQALHVPGSPR"
     gene            15084..16082
                     /locus_tag="PLQ30_04155"
     CDS             15084..16082
                     /locus_tag="PLQ30_04155"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPW01669.1"
                     /translation="MLYTFDFHNHSCLSPCASLEASPSEMVEWAIKKRIDLFALTDHN
                     SALNSPAFGIECANAGIIPLFGIELNPFEEAHLLAIFSDPLTALRFSDWVFEYLPAMR
                     VDPLQFGDQVVVDPLGQILSMPSSTWYGSSLKESFTFFAQEAAKAGAIVIPAHIDRPH
                     FSVYSQLGFLPQGPYDAVEAMSPNPDPTLCGDFCVISNSDAHVLEHIGRRPSIIELDE
                     KKVIDMHAALSKMAEAWFRGLWTNQANISASSPEKESEEDSEEDIQDQTSEAGLSIEI
                     PGLLPIISFLEEWYPFAEAQALFENIRQSLHKQKAWSIYKKPKPGSLIHASAEKKK"
     gene            complement(16051..16827)
                     /locus_tag="PLQ30_04160"
     CDS             complement(16051..16827)
                     /locus_tag="PLQ30_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01670.1"
                     /translation="MRLGANQKRNGWRSVVSFISIIALIVFISFSVTSCSGNLDATIK
                     GDSSVRAAIRLDIPDALSVRVRQFVGLKPQEPLFSTEAVRKQFLGQTNIALVDVSSPT
                     PDILTSVVWVQNLEALIADTSLVPMGMICYQKISPPGQSPAMRELSVNLSRENAPAML
                     KLFPGIDRRIIDSLSPPALEADPVSAAEYRLNLEQLIIGKKNMPAFDACAVNIAITTP
                     KMIVSATGGSISGTVFRMRIPLFALLTLEKPISFSLRWHE"
     gene            complement(16845..>18210)
                     /locus_tag="PLQ30_04165"
     CDS             complement(16845..>18210)
                     /locus_tag="PLQ30_04165"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPW01671.1"
                     /translation="SMQFSWKRFADELFYYSNQFILFFVLIISLTSGSWKNALVTSLI
                     VLSFMLIQTSLLTIQGHIPILRFLYSFITAAGYSILRATLSDIAFTETVNLLLWGASV
                     YIAVFQTLTLILHKPILKRFSEAALSLGSAIIFLLFYAYLDLRISVTASFEKGQIDAE
                     ALAQALSLKSFLAVFDKFIQAPQHLFALFGVISFDFMLMVARWRAINLEQRLTRIIEM
                     PQEAKKIYISSLEPETDASSAESESQEVQLAASPENQENPPAKSAKTVASLFVTVISS
                     DIIGFTDLSEQFGHKEAAALLNRYYAIWTRCAGSYGGRIISISADTVVIVFGLVDEEH
                     SADRALNASYAFIEELEGLKEDMIIKSLPGEIKVSLGIHSGYVTRALLGPPGQQKMSF
                     FGDTIGIAARLDSLCREFHQNLLVSHATYRHLTLESQVTLERFSEALLRKSTRPMPLY
                     TKKP"
BASE COUNT         4836 a         4017 c         4208 g         5149 t
ORIGIN      
        1 cccaaggatc accaagaccc aaggatcacc aagaccaccg gggactataa aaatcgatgg
       61 ttaagaggct tctagggtat tttcgcgacc tcgacgatct tatactgagt ttattcttac
      121 aagagcatcc ctgctcggaa ttgaagcgag atgatcttgg aacctgaata acttgcataa
      181 aaagtcatgg ttgcaggagc aagtccaaaa gcgcgagctt caaatgaaaa ggacaatcct
      241 gcagcccaat cgttcatatg gaaattttca tccgatgtgc tattccagcg cgtgtcgaag
      301 aatggagcca gctcaaagcc ttttaggagc aaggcttctg aaatatcaag aaataattca
      361 tggggcgaaa atgggatttc cacatttgtg atcaaattgg agtttactgc ttttcttgtt
      421 gctatactcc taaatccatc gtatttggat tccatgaact cctggttatt gagtgacact
      481 ttcgaaaaag cacgaaactt ggggctcacc catttggaat caaatcctcc ctggagagaa
      541 ccttgtaaac ccactgcgtt ttttaatcca acagaatttt ccgcaaaagc tcctatatat
      601 gctgagtacc atggctggat agaggggcct gatttttcgt attttctcgg catctctgtc
      661 tggaaaaatg cacatgtctg ccaattcgcg atcgaaaaat ccattttgaa aaagatatcg
      721 cctcctatat gaaatcctcg ttttctttct tcttgtgctt tcatccagat catacggctt
      781 gctaactccc aacagctgga gtcatgcgat aatatcgagg ttgagctata atccccccat
      841 agacgggcag taaaccatgc attgaggctg tctagcggat ttacacgaaa ggcagtctct
      901 attgcccaca cctttcctgg atctgggtta agagcattga aataagataa ataggacttg
      961 aacttcaaat aagagaaagc aagagatgcc cctatgcgtg cctctgtccc cgtctctcct
     1021 ggcatggata taaaaggatc aaaaagcctc agaataggta tgcctctcag catgagggag
     1081 gtggagggtt gttggccact ttggaggtct tgaatggtat tgttaagatt ctcatgcatt
     1141 tcgctgcgca attcgaagaa ttcaggagaa ggagggtttt ctagatcttt gcgagaaata
     1201 agatctaatt gctcgagagc attctgtgcg ctcaattcac ctttttctat aattatatca
     1261 gggtcagtaa attgcatata agataggttt tccacatcgt tgcggattac cagggccttt
     1321 gatctctcta ttcctgccat acctgatcga gtttttccga tatctacagc tcggttaatg
     1381 accgtgactg gactagaaaa atccatcgct cggttataaa aggcggttga aatgacaagg
     1441 cggtcgctga atatgagggc aggttctaca ggcgcaatgg tcgtggctcc tgcatcgatc
     1501 aggacaaaat tgtcgaattc ttgaggttcg aaaatagcgg gcattgcaaa cgcacttgcc
     1561 attacaagat caaatggacc ttctgcgatc catatctgct gtcgagattt gagatcttct
     1621 gctattatga taataggaat cgctgtctca gaaacatcga gtcttccgat aatcttcctc
     1681 agaatagcct tgaacatatc ggtattgatg agaccaccat ttgtgggtat cactggagta
     1741 aagaaagact caagcggaag cgatttgatt ataaattcga tgtcttgggg tgaatatcca
     1801 gcagcgtata gcatgccgac aacagctccc atggagcttg caacaatgaa atccggataa
     1861 atgttggcct tttccaatat ttttaggacg ccgatatgcg cataagctcg cgccgatcct
     1921 ccacagagaa caattccaag agggggtcgc ggctcgctgt tttggttctt atcgaagtcg
     1981 gggaactccg ttctttgctc tagccttgca atgaatgtct tttctcccaa gaacactggt
     2041 tttgtgatat ttcgaagagc atctccttcc gggaaagcga attcaaaatt atcaaaagat
     2101 ggaagctcgc cagaaaaagg ctggctttgc tgagcttgaa gattcaaggt tgataaacat
     2161 attaagataa gcgatagaag cctagatggc atatgcctgc gatttctatc aagaatagtc
     2221 ttcatttact gtcagtatat attgatatta gatgaagacg gaagagtatt tttgagaatt
     2281 tttaaatacc gtaggtcttt ttcagctgtc attgtggctg tgtgattttc cgatggctct
     2341 tatgagtttt ttcaagtgaa gattatcgga gcattgttaa tgcctcgaca atggcgatgg
     2401 atatttgtta ttctttttat atggaaagcg atagctcatc ttcaagtgac tctgactatt
     2461 tcatatgcat tggtcctaaa tatgttgaat taatggcaat agctcctata tctggagacg
     2521 aggtgcgcaa tgatctctga aatcctcgtc ctattagctc tcatttttat taacggattc
     2581 ttttctctct ctgagacagc cctcgtttca tctagaaaag ccaggctcaa ggctgaggcc
     2641 gacaaaggca agagcactta caagcttgcc cttaacacgc gggaatctcc aagccgttat
     2701 ctttcgacaa tccaagtagc gattacactt attggcatac ttacgggcac aataagcggt
     2761 acgacttttt cgcagcttct agctcaatgg ctctcgggat ggtcttttgt tgggagatat
     2821 gccgaatctg tagctgtggc tattgtggtt ttgaccataa cctttctctc tgttgttatc
     2881 ggtgaactcg ttcctaaatc catagcgtta cgcaatcctg agtatattgc tgctctgaca
     2941 ataaagccta tgcatgcttt gagcgttgtt ttctatcctg ttgtaagatt tctctcttct
     3001 cttaccgata gtcttacgaa gcttttcaga ttctcgcatg gagccgagcc tgctatcact
     3061 ccggaagaag taaggatcct attagaacaa ggagaaaaat caggggtatt tgagacagca
     3121 gagcgagaaa tgatggaaga ggtgttgaat cttgatgata ggcgagtaac gatgttcatg
     3181 acacctagaa ccgaggtagt aacgttgaat ataaaagatt ctcgagactc tcaaataagg
     3241 gctattatca agaattccga atatgcctat cttcctgtgg ttgatggaga cttggatcac
     3301 acaataggta tggttgcagt gaagcgagca ctcacatgta tggcggaagg ctgtttttta
     3361 tctttggaaa catgcatgga gccagctgtg atgatcccgg agtcattatc gggcttgcaa
     3421 gcgcttgcta ttctccgcga tgccaagagg tcggctggtt tgatagtcga tgagtttggg
     3481 ggaatctcgg gtattgtgac aattgctgat ttactggagg ccattgcaaa tctctcggaa
     3541 gtagagacag aagaggcttc tcagattatt aagcgcagtg atggctctta tcttgtggat
     3601 ggttcgatgc caatcgatga atttgccgag agcataggta tcaatctgga tgtcatatca
     3661 aacgaggatt acgacaccgt tgctggtttt attctccatt gtgggggatc aattcccaaa
     3721 gccggagata taatagaatg gcctcctttg aaaatggaaa ttgtggacat ggatggcaaa
     3781 cgtatcgata aagtattggt acagaagtca gagcaatctc ttacctaggc tttttctagt
     3841 ctgcacaaat aacgcataga ttggaaagtc cgatagtcca aagattccac tctaatgccg
     3901 ttttttgaaa atccagtgtt tttgaagcat aaaattgtag aatatcgata caacaacatc
     3961 taccgcaagc cttgtgattt tatagtctat cttgcttagg gttgtaaaag caaaggttcc
     4021 agctgacttt agggcgatac tgcctagaac cactgggaag aacatgacaa gttgttggat
     4081 tacagaaaac tcatattgcg catcctttga atagaaactc catgttctgt ttacgctgaa
     4141 atttacggcc gcgcccacca tacctccggc tgcgatcgaa atagtatagt gaatatgaaa
     4201 gacctctgta atgaatatca ttgtacagta atcaattact ccgccaacaa aagcagatat
     4261 ttgtgctttt gagaaagtaa gggctgaacg tagccaagcc aaagcttttt gtctattctt
     4321 tattattttc ggcaattttc aattcactct ttctgttttc ttcttcatca cgctcattgg
     4381 cgatttttgc tagtttttta aattcagaaa tattggaaat cgctaagata atatcggcta
     4441 ttgcggtaag gtagacaatg cttcctggga aaataacttc aatcacaagg aaaagcgaga
     4501 taagaatcct ggtttcggtc gggctcaatt taccggcgtc aatggaatag ctaccactga
     4561 ttttatatcg aagcagagct attatgattt ctagaccata aagcacgata aaggcatatc
     4621 ccaagagttt tagaaagcgg tctgcgtaga taacgaatcc aaggccgata agtacgatac
     4681 ccagccaatc tatcacaaga tctagcataa aaccgtacca ttttcttggt tttttcctat
     4741 aataggctat tcgtccatca agtgaatcgc cgaaccagga aatcgcaaaa ccaattacac
     4801 caatcagaag aaagcgagtg tcaaaaagct ctgcgagaat gaagctcaaa aaaacgatca
     4861 catttccaag aaaccctatt cccgttagaa cattggagct catccatgaa ggtatatatt
     4921 gaaccagaaa ggcaagggct ttttgctcct gccttttaag cagatttgtt ctaacgcggt
     4981 ccttggaaat aagcggtaga gcatgatcta ttgagttttt cttttgtttt attttggttc
     5041 taccatgatc tttattcatt ctctgcctca acatctctct ttacttcagt tgaaaaagtt
     5101 ttaacaatta tactcaatct gattttcgta tggcaacggg gtataaaagt atctatattt
     5161 taggaatgtg tcaataaaag ggctttatat tatttggagt atgagattat ggacaagaaa
     5221 agattctttt atgtcgctat ctatgcgatc gggatggcat atctcgaggc atcaattgta
     5281 gtatatctga gaaagttgta tggcatttct gatttgatga tcagtgtgcc agccttcgat
     5341 cccatggtat cgatcattga acttgggcga gaattggcga ctctcattat gcttctcgct
     5401 gttggacttg tatcaggcaa gaaatttcaa tcccaaattg gtttttctct gtatgctttt
     5461 gggctttggg atatttggta ttatatatgg cttgtcattt tcatcggatt tcctcaatca
     5521 ttgtttgatt gggatctgct ctttcttatt ccactaccat ggtgggggcc tgtgattgcg
     5581 cctgtgatga tatcggtgct catgataatc agcggagtaa ggcttgcttt gcttgaagaa
     5641 aaaggtctcc ttgtaaggct taaatggttg gattgggcaa tgctcgcttt aggatccggc
     5701 accatgctgt atgcttttat gagcgaagca atttcaagtt tgccagcgag tgtggaaaat
     5761 ttgagcgatt ttagaccaag ctcattctta tggccaatct tccttatagg atatgcaata
     5821 tctatatatg caacttggag aatactatac aaaagtctga atgaaccagc gatgcacagg
     5881 agcgtcagga agtagttcgg ggtgaaatgt cgcggcaatg atcgagccct gacgaaccat
     5941 tacaggctca ttttcatata ttgcaaggtg ttcgacctct tttccggaag cgagtatacg
     6001 cggtgcccgt atgaatatgc cttctattat gcctacgctg tcaatgatgg tttgtacggg
     6061 agcatgaaag ctatctactt gacggccata tgcattgcgc ttgactttga tatccaggac
     6121 accaagtccc atttgatcat ttccttcgat gttttttgct aggagtatta gtccggcaca
     6181 ggttgcgaga acaggcatcc cattctctat ttggcgcttt agttcttcga acatgccaaa
     6241 acgttcgagc agcatactca tgaccgtgct ttcacctcca ggaattataa ttgcggtcaa
     6301 ggactgcaga tcggaaaagc tccgaatctc caccatatcg cagcgcggaa atccagctcg
     6361 cacaagagcc ttaccatgag cttcatagtc tccctgaagc gcgagaagcc ctatgcgatg
     6421 gctgctcacc aacccctccc cgaaattttc tgattttcga taagttcatc caggccaatg
     6481 ccaaccatcg gttcgccaag atcttccgag atctccgcca ggatttttgg gtctttgtaa
     6541 ttgaccactg ccgatacaat agcttttgct cgtcgctcgg gattgcttga tttgaagatg
     6601 cctgagccta caaatacgga ttccgcacct agttgcatca tcagagccgc atccgctgga
     6661 gtagcgactc ctccagccga gaaattgggg acaggcaatt tgccagtttt tgcgacttcc
     6721 catacaagtt caaatggagc accgagctcc tttgcttcag tcatgagttc ttcctccgga
     6781 aggccaagga gacgagcgat tccatccata actgtgcgca tatgccgcac agcctcgatg
     6841 atatccccag taccagcttc tcccttcgtg cgaatcatgg ctgcaccctc gccaattcgc
     6901 cgcagtgctt ctcccaggtc acgacaaccg caaacaaatg gtactttgaa aggatgttta
     6961 tagacgtggt agcgatcatc cgcaggggtg agaacttcgg attcgtcaat gaaatcgatg
     7021 ccgagagcct ctagtatctg tgcttctaca aaatggccta tacggcattt tgccatgact
     7081 ggaattgaga cagcatcctg gatttctttt atgatcttag gatcggacat gcgggcaacg
     7141 cctccctgag ccctaatgtc ggctggaacg cgctcaagag ccatcaccgc acaagcccct
     7201 gctttttcag cgagttttgc ttgggcagca ttggttacat ccataataac gccacctttg
     7261 agcatttcag cgagtccgac tttaatatgc cattgctcgc tctggcgatc ggaccataat
     7321 ttctgttcca taatataatc tcctcactgt tagctgtgtg gaaggaattt gattggttgc
     7381 aagaataaag agtagcgaaa aaaagcttat tacgcaaggg ttgtggcaaa gctctaggat
     7441 tctgatttat gagatattgc gctccaggag tattcttttt atattcggtt tttgagaatg
     7501 tctatggagt aattcttgct gttacctaag cgggattagt cctcgatgtc gaaaatcttt
     7561 cctggattca taataagatt tgggtcccag gcgcgtttga tcgctttgat tagattcagt
     7621 tccacagggc tcatgaattt tctcataaaa tgtttgcgtt taagtccaat gccatgctcg
     7681 ccggaaattt ttccgccaag cgcagtgatc gattcatata gcgcaaggag agcttgttgc
     7741 tctatttgtt gccattgctc cattggcatt tgcggatcct tgactagagt cgcatgaaga
     7801 tttccatcgc cagcatggcc ataacatggt attgcaatac caaattgcaa agcaatgcgc
     7861 tctagttcgg gcataaccgc agatatggcc gtgggcggca ccacgatgtc ctctaggctt
     7921 tgaactgggc aatagacttt taatgcttcg gctatgtttc tgcgaatagc ccagaggcgt
     7981 tcctgggtag ttcggttgtc ggctacgtat acttcgattg caccctgctc catgcataaa
     8041 tctccaactg cttctgcttc actttctact agagattgat ctcgtccatc catttctatc
     8101 agcagcatcg caccgcattg ggcataaggc aaattttcgt ttagataggt acaggatgtc
     8161 tccacgctcc ggcggtccat gaattcgatg gatgttggaa tgagaccctt tgagagaatc
     8221 ttggggacag aggaaatggc ttcttggggg cttctataga gtacaagcag gtcggatttg
     8281 gctgccggaa gaccaatcag tctaattata gctgcggttg caatgccaag agtaccttcg
     8341 gagccgacaa tgaggtgcac tagatcatag ccagtaacat cttttgcaag cttgcctcca
     8401 agccagacca cttcgcctgt tggtgtaacg aattctagac caaggatata gcgcgatgtt
     8461 acgccatatt ttatggcttt cccaccccca gcattctcgg ctatgtttcc gccgagcgta
     8521 caggtctcca ggctcattgg atagccagcg tagaatagtc ccttttcctt gaccaggttg
     8581 ttgatttcgt ttgtcacaat accagtttca acggtcattg taagattgtc gtaatcgatt
     8641 tctcgcacct ggttcatttt ttcgagcgac aatactattc cgccaaatat aggaatagct
     8701 cctccagaaa ggccactgcc agcgcctcgt ggtgttatgg ggacagaggt tcgattagca
     8761 agtttcacga tctcagcgac ggcggaggtc gaccttgggg tcaccaccac ttccggcata
     8821 tggccatatt cctctttaga tgtttcatcg tgtgagaaga tttcgagttg agctggatcg
     8881 agcaggacgt ttcgctcacc tgcaatattt ataagttcgc ggattatatc tctgctgata
     8941 ggattatatt tcatgctgag cctccgtgct taaggttttt gagtccaaaa ttcttggctc
     9001 ttgagatctt gtatctttgg tttggtatgg agatgaagag agggatggat aagaagagct
     9061 atagatcggt tttaatcgcg ccgttagccg cggcaaaatc tcgaagaggt cccctattat
     9121 ggcgaggtcg gcaaccgcat ggatgggcgc gtcggggtct gtgttgatcg agattatctt
     9181 cttggccgtt cttatgccgg ccagatgttg aacagcgcca gaaattcctg cgcatatata
     9241 gatctcgggt gaaatggtct tgccgctaag gccaacctga tgtgggtagg atgtccatcc
     9301 gcgatcgaca gattcacggc ttgcgccaac cgctcctccg agagccgacg ccagctcacg
     9361 aatgagcttg aaattatcgg ctttctttaa acctcttccg cctgcaacaa cgatgctcgc
     9421 actttccaga ttttcgaaat ccttaacatt gctctccaga gccagaacct ggacacgtga
     9481 atccgaaaaa tccgagatta ttctctcctt agatgattca tcgattacat gaattacaat
     9541 gcctttgcgg ccaaagtctg gcggaagtgg tgacatggag tgaggtctta ccgtggccat
     9601 ctgtggcttg tgatgggcag tttttatagt ggccatgata tttcctccaa tagcaggacg
     9661 tgtctgcaaa aggaggccgc tgttttcttc gatagagagc tcggtacagt ccgcagttag
     9721 tccggtgccg agtttggctg aaagatatgg cattaaagtc cttcccgttg tagtggccgc
     9781 tcctagaatg acttcgggtt ttctggcgta agcaagcttt aagagcgttt cagaccacaa
     9841 ttcacagaca aaatcctcta ggctcgggtt ctcgaccagg attacactat cagcaccatg
     9901 ggcaatcaac atcgcagctt catctggaga tgcggatcca agcacaatgg ctgctagaat
     9961 tgcaccacga ctatccgcga gaggacgggc tctagccaac aattcgaaag acactatgtc
    10021 gagtccgcgc ttacggcgct cagcaataat ccaaaattca ggcttggggt cagtagcagg
    10081 ctccgaaaaa accttttcgg aaagagaaat cactgattcc tgcaatgcgg gctgcatttt
    10141 tgctataagg actccaatcg ccgattcttc gatatccaca gctggacttg tttctgtgta
    10201 tcctctcctg gattctctgg atcctctgtc ccccattatc ggactgccta ttacaggacg
    10261 gatcattgaa agaatgcgtt caatcgcttt ttctattccc ggttcatcga gagcatgaat
    10321 tcgctccccg tcgcgtgata ctttcggata aaatatcttt actacacggg ttggagagcc
    10381 agccagaccc aatgaagcag gatccaggtt tatttccttt gctccccagt gcaaaatctc
    10441 tgcactcctt gagaattttt tcccgcgcag agttggtaga cggggcgagg caatttcttt
    10501 gactacggtt attagacatg gagattggac tctcacactt tggtagcctt cttcggtaag
    10561 gcgacggacg agaaatgtcg gatggcaagt ccgattacga tttgattcag cagtttctaa
    10621 atcttcagtt ttggactcta tttccaatga actcacatag gtgaggacgg gtatttccaa
    10681 ccaagatgct acggctggtc ctacttgtcc ggtatcgcca tcggtagcgc gctctcctgc
    10741 gaggattaga tcggcgttgt caatatgcct tattgcctca gctaaaacct tcgatgttgc
    10801 ccaagtatcg gacccggcaa aagccttatc tgtaagtaga attgcttcat cacaacccat
    10861 tgcaagcgat tctcgcagcg agcgttcggc atctagtggc cccatcgaaa ttgtggttac
    10921 atgggctccg aatcgctctt tcagtatgag tgcttcttcg attgcatata gatcaagagg
    10981 attgattatg ctttccagcc ccgatcgcac cattgtgcct gtatcagggt ccatcttgac
    11041 tttatctgtt tctggtactt gctttataag gacaatgatg tgcattgaaa aactccggat
    11101 tgttagtttg acacaagagt atgacacaaa agtatgacat aggagtagat atatccgcaa
    11161 gcttgcactt taataccttt gggagacatg aattggagag gaattacaat tgtggtgctt
    11221 gccttatcag ataatcgttg aatatcttta atcctggcca gacatagatt tatctcttca
    11281 acctggattt aactaatctg atcgatttta ttatatttat gtttgttctt gtttccattt
    11341 catgtgggcg agtcatttat ttttctgcac ggaggaggaa tatgacaaag aacatgggaa
    11401 agacagacaa gagggttcgt attattgtcg cagtggtcat tgctattctt attctaactg
    11461 gagtcctaaa gggtgtgctc gcatggatcc tcggaattat tgcggcattc tttgtggtga
    11521 ctgccgcaat atcgttctgc ccgctatata ctctctctgg ttgttcaact attcataagg
    11581 aaaagacaga aaagaaataa gacaaaaatg gttcaaagag cttcttgagc aaggcgacaa
    11641 agatgaccgc caatcggtta tggcggtcat cttgatattc tgcccttaat ttaaagtatc
    11701 atgagaggca gataaggatt cttcctgccc cgcctccgga gatggtgaag ccttggtttc
    11761 ttgtgtttct tttgctagtt ttctcgcctc actagttttt cttttctgtg cagatagcgc
    11821 tcgcatgcgc ttccatatcg acggaaaaag cagaagcata ccaattagaa tccctgcggc
    11881 gaaggtcaac actagaacaa tcgacatcga agcctgggca gaccatccaa aaaacgaaac
    11941 ggtaatgggt gcgctatttt gtaccgcaaa gatgacggcc aaaacagcca cagcaatcag
    12001 aagaacaatg taaaagactc tcattttccc tcctcatatg gagtttctta agaaggtctt
    12061 tactcttaat ttatggtact gtcgatggta tcagatttcc aatgctcagg agcgcgccag
    12121 agccccgata gtaagagttg ccgaacgaag atgagctctt ttggcaggcg ttcatagagc
    12181 tttacaaaca gctcctcatg agaaagaatt tcattctccc agagctcgcg gtccacagac
    12241 atcagctcaa taaagcgctc ttttggaaaa tcgagacctt cccagttcaa atcttcatag
    12301 cgtggcatcc agccaatagg gctctcgatt gcattggctt tgtgattggc gcgatcgaca
    12361 atccacttga gaacgcgtat attttcacca aaacccggcc atatgaagtt accatcggca
    12421 tccttgcgga accagtttac accgaatatg agtggcggat tagggttgta aaggccaaca
    12481 tgcagccaat aatcgaagta cgaagccata ttgtaaccac aaaatgggag cattgcaaaa
    12541 ggatcccgcc gcacaagccc aatggcgctg gtagcggcag ccgtagtctc ggaaccaagg
    12601 gtagctgcaa gatatactcc atagatccag ttgaaggatt gatagaccag gggatagacc
    12661 gtgcttctgc gtccaccgaa tataaatgct tttattggca caccttcatg actatcccag
    12721 gcggagtcga tagaggggca ttgcttaatc ggagccgtaa atctggcgtt tggatgggcg
    12781 gctggtttgc cggaggcggg atcccacggc cttccctgcc aatcgattaa gccctcaggc
    12841 ggattcttgg tcatgccttc ccaccatata tcgccatctg gggtgagagc acaattggtg
    12901 aaaatggtgt tggcccggat agtcgccatt gcatttggat ttgatttttc cgaagtccct
    12961 ggggcaacgc caaagaaacc agattctggg ttgatggctc gcatttgccc gtctttgcct
    13021 ggacgaaccc aggcgatatc atcgccgaca gttgtaacct tccagccctc aaaggactcc
    13081 ggcggaatga gcatagcaaa attagtcttg ccacaggcgc ttggaaaggc agcggctacg
    13141 taggtttttt catgagaggg tgattcaagg ccaagtataa gcatgtgttc ggccagccaa
    13201 ccctgatctc gcgcaatgac agaagctatc ctcaacgcaa agcatttctt accgagaaga
    13261 gcattgccac catagccaga gccatacgac cagatttcac gagtctctgg aaaatggaca
    13321 atgtattttg tctgtttgtt gcatggccag gcgacgtctt tttgtccggg ctcgagaggc
    13381 atgccaaccg aatgcataca aggcacgaat tcaccatttt cgccgagagc ttcgatgact
    13441 tttctgccca tccttgtcat tatgcgcata ttggcaacaa cataggcaga atcagttatt
    13501 tccattccga tatatgaaaa gggagaacct ataggtccca ttgaaaaggg aatgatatac
    13561 atcgttcgac ctcgcatgca gcctgcgaag atcttgttga gaatacctct catttcgtta
    13621 ggctctaccc aattgttggt gggtccggca tctgctttgt ccttgctgca gatgaaagta
    13681 cggtcttcaa ctcgagcaac atcgctgggg tcggaccttg acaagaaaga atttgggcgc
    13741 ttttcgggat taagtgcgat gaaggttcct gacgcgacta gagatcggca aattgtatca
    13801 tattcttcat cggaaccatc gcaccaatag actagatcgg gtttgcacaa ttcttccata
    13861 gaggaaaccc acgaaagaag ccgttcgttt tttacataag aaggactcat cttgagcgtt
    13921 ttcatgaaat tactccgaag aataaagata taaatgctat tataggcttt ttttttattt
    13981 tgtgtatggg gaaggtcttc aatgccaaat ttatcatcgc cggagcaccg tcttttcttc
    14041 tgcatgaaca tcatttatgc taaacgcatg aatagccttt ctgtacagac acaatattgg
    14101 ataatggcca tatcgattat tgttttcgcc ataaccatta tcacagctgt tttggcctac
    14161 aaagccggca agaaacatgg attcttagat gcgcaggtct ctgagaatga aagaatggag
    14221 catgcgcgaa aagatgcgat agaacgatca agagccgttc tttccggtca aatttcggag
    14281 caacttgcgc catggctccc cgattttccg gtcaaccctt ccgacgcaag gtttattggc
    14341 aagcctatag atttcgttgc tttctgtggg acagatgagg gatctgtgag cgaaatcgtc
    14401 tttgtagagg taaagtccgg aaaatcggct ttgagttatg tcgaaagaag cgtcagggat
    14461 gcggttgtcg gaggccatgt gcgctgggtg gaatacagaa tcgaatagaa aacaaatcaa
    14521 agaatgccaa ggaaatagca aggccttttg ctttgttctg gaaagcattc ttccagcttt
    14581 attgaaatgc aaagtgcggc tataatataa gtaattctat tgccattcgc tttgccatac
    14641 cgaaatcgat ctgatgcaag ctttttattc aaatgatggc aaagccgaaa tagaagcaag
    14701 aacgaaggag taccattgca tgacgactaa agatgttccc gatatcattt ctgctgaagt
    14761 acttcaagga gattttgaag acagagcagt caagatagtc tatacttctg atttgctgtc
    14821 cgatgtactt gcaaatgggc gcgatgcaag tgtgcttgtc acgattcagg cacataagaa
    14881 tacggttgcc gtggcgagtg tagtcgatat tcctctaatt gtcctgtgta ataatcggcc
    14941 aatcatagat gatatgatcg agacagctgc tcagtctcga ataggattgg tgcgaacggc
    15001 tcttagtcag ttcgaagttt ctggtcggct ctggcaggct ttgcacgttc cagggtcacc
    15061 aaggtgatgg acatatattg tccatgttat atactttcga ttttcacaat cattcctgtc
    15121 tcagtccatg cgcaagtctt gaagcttctc cttcggagat ggtcgagtgg gctataaaaa
    15181 aaagaatcga tctatttgcg cttacagatc ataacagtgc actgaattct cctgcatttg
    15241 gtatagaatg tgcgaacgcc ggcattattc cattatttgg catcgaattg aatccctttg
    15301 aagaagcgca cttgttggca attttttcgg atccattgac cgcgctccgc tttagcgact
    15361 gggtttttga atatttgcca gccatgcgag tcgatcctct gcagtttggc gatcaggttg
    15421 tcgtagaccc tctagggcaa atcctctcta tgccctcttc aacttggtat ggcagctctc
    15481 tgaaagaatc ctttactttt tttgcccaag aggcagcaaa agcaggcgcg attgtaattc
    15541 cggcgcatat agaccgacca catttttcgg tttacagtca actcggtttc ctaccgcaag
    15601 gcccctatga tgcagttgaa gcgatgagcc caaatccaga tccaacactc tgcggagatt
    15661 tctgcgtcat ctccaattcc gatgcgcacg tgcttgagca cattgggcgc aggccttcca
    15721 taattgagct cgatgagaaa aaagtcatcg atatgcatgc agccctttca aaaatggctg
    15781 aggcctggtt tagaggccta tggacaaatc aggcgaatat aagcgcttca tcaccggaaa
    15841 aggaatcgga agaggattcg gaagaagaca tccaagatca aacatccgaa gccggccttt
    15901 cgatagaaat cccagggctt ctgcctatta tatccttttt ggaggaatgg tatcccttcg
    15961 ctgaagcaca ggcgcttttt gagaatatca ggcaatctct acacaaacaa aaagcctgga
    16021 gcatatacaa gaagccgaag ccaggatctc ttattcatgc cagcgcagag aaaaagaaat
    16081 aggcttttcc agagtaagaa gcgcaaacag agggattctc atgcgaaaaa cggttccaga
    16141 tatgcttccg cctgtagccg acacaatcat cttaggcgtt gttatggcaa tatttacggc
    16201 gcatgcatcg aaagcaggca tgtttttctt gccaattata agctgttcga ggttgagccg
    16261 atattcagct gccgaaaccg gatcggcctc cagcgccgga gggctcaagc tgtcgattat
    16321 tcgtctgtct attccaggaa aaagctttag catggcgggt gcattttctc tcgaaagatt
    16381 gacggaaagc tcgcgcatgg ccggagattg ccctggtggt gagatttttt gatagcaaat
    16441 cattcccatt ggcacaagcg atgtatctgc tatgagagct tcgagattct gcacccatac
    16501 cactgatgtc agaatgtctg gcgtgggcga agaaacatca acaagcgcaa tatttgtttg
    16561 ccccaggaat tgtttcctaa cagcctcagt gctaaataga ggttcttgcg gtttcagccc
    16621 gacaaattgt ctaactcgca cgctcaaggc atcgggtatg tctaggcgaa ttgccgcacg
    16681 cacagagctg tcccccttga ttgtagcgtc cagatttcct gaacaggagg tgacggagaa
    16741 agagatgaag acaattaagg caatgatcga aataaatgaa acaacacttc gccatccatt
    16801 acgtttttgg ttcgctccta atctcatgac aaatcttgct ccaattatgg ttttttagtg
    16861 taaagcggca taggccgcgt actttttcga agtagtgctt cgctaaaacg ctcgagcgta
    16921 acctggctct ccaaggttag gtgccgatag gtcgcatggc ttacaagaag gttctgatga
    16981 aattccctgc agagcgagtc cagccgtgcg gcgataccaa ttgtgtcacc aaagaaactc
    17041 attttttgtt gacccggagg tccaagaagt gccctggtta cataaccaga atgaatgcct
    17101 aaagaaactt tgatctcccc aggaagcgat tttataatca tatcttcttt aaggccttcg
    17161 agttcttcga taaaggcata tgaagcgtta agggctctgt ccgcgctgtg ttcttcatcc
    17221 acaaggccaa agactataac aaccgtgtct gcggaaattg atatgatcct tcctccataa
    17281 ctaccagcgc atcgggtcca gattgcataa tatctgttga gtaaggcagc cgcctccttg
    17341 tgtccgaatt gctcagaaag atccgtaaaa ccaataatgt cagatgaaat caccgtcaca
    17401 aatagagaag ccacagtttt ggcggatttg gctggcggat tctcttgatt ttcaggagat
    17461 gctgccagct gaacttcctg gctttcagat tctgcggaag atgcgtctgt ttcaggctcc
    17521 aaagaagata tgtaaatttt tttagcttcc tgtggcattt cgattattct agtgagccgc
    17581 tgttccaggt tgattgctct ccatcttgca accatcagca tgaaatcgaa ggatattaca
    17641 ccgaatagtg caaataggtg ttgaggggcc tggataaatt tgtcgaagac agcgagaaaa
    17701 gattttaagc tcagggcttg tgccaatgct tcagcatcta tttgtccttt ttcgaatgag
    17761 gcggtcaccg atatgcgaag gtcaagatag gcataaaaga gcagaaaaat tatcgccgat
    17821 cctaatgata gtgctgcctc tgaaaaacgt ttgagaatcg gcttatgcaa aataagcgtg
    17881 agtgtttgaa aaaccgctat atagaccgaa gctccccaca acagcaagtt tactgtctct
    17941 gtgaatgcta tatccgacag cgttgcgcga agaattgagt atcctgcggc cgtaataaaa
    18001 gaatacaaaa atctcaggat tgggatatgc ccttgaattg tcagcagtga ggtttgaatt
    18061 aacataaagg aaagcacgat cagcgaagtg acaagtgcgt ttttccacga tcccgaggtg
    18121 agcgatatta tcagcacaaa aaagagaata aattgattcg agtaataaaa tagttcatct
    18181 gcaaagcgtt tccaagagaa ctgcatcgaa
//
LOCUS       DAOMZL010000032        17914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_2785_length_17914_cov_15.325102, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000032 DAOMZL010000000
VERSION     DAOMZL010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 17914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17914
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2785_length_17914_cov_15.325102"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1628)
                     /gene="lpdA"
                     /locus_tag="PLQ30_04170"
     CDS             complement(135..1628)
                     /gene="lpdA"
                     /locus_tag="PLQ30_04170"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPW01672.1"
                     /translation="MSDYDVIILGSGPGGYLAAERLAANKIRVALVEKTAVGGTCLNI
                     GCIPTKTLLNSAKLYAHLRDASSFGIETEGVHINWRQVMAWKEEVSKRLRRSLELTLK
                     KAGVEIIRGVGKLTDGHSVIVFPVNAESDALQPSLYQAQTSQSSDYQSPDSQSSNSAF
                     MIRGQKIIIATGSSPIIPPIRGIKDNPAVLDSTGLLSIGEIPSSLCIIGGGVIGIEFA
                     SLFSAFGSSVTVIEMMDEIVPVMDSQMAVELRKALKNVTFMLGTKVTELEGKKVLFEN
                     KNGEKGSTEADIILLSVGRKANIEDWGAQESGLEIKNRAIATDDRMRTNLPGVWAIGD
                     VTGKSQLAHAAYRMAEVAVSDILASQNSERSNQIFVPETVPWALYSLPEAAGAGLTEQ
                     DARARGYDTHVIRMPYNVSGRFVAENGFSAPGSIKIIIEKSSERLLGIHLLGAYASEQ
                     IWGAALALERRLPLSAFRNMVFPHPTVSEVIREAAWSANPLSGTHIH"
     gene            complement(1695..3002)
                     /locus_tag="PLQ30_04175"
     CDS             complement(1695..3002)
                     /locus_tag="PLQ30_04175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HPW01673.1"
                     /translation="MAEKIIMPKLGNTVESSVILSWKVKPGDTVTKDTILCEIETDKA
                     TMEVPAGVDGTVLALLKREGDDVPVLEPIAIVGAPEEVWENAADSSQANTASIPISAS
                     TSTVFEQEKEATMLKEANGKEPHSSPRARMAMHNLGVDLGAIDKGSGPGGRILEKDVI
                     EASIDEKSNQEVSMQEPPIQGAFGKILQATQTQTIKEEPPTHEKTEFQSIPLIGIRKR
                     IAGRMHDSVQTTAQYTEHSSADAEHLVALRARLKAQENPNIAHITIGDLVLTAVIKIL
                     PEFPEFNAHLEGNELRLFKPIHLGVAVDTPRGLMVPVIRNAQDLSLLGVSMEVKRLAK
                     ACQSGRADPASLSGSTFTVTNLGAFGVEWFTPIVNTPETAILGVCTIRPTPVKTDKGI
                     ENRMRIGLSLTVDHQVIDGAYAARFLRRLSEIIADIDIMPFSL"
     gene            complement(3036..3791)
                     /locus_tag="PLQ30_04180"
     CDS             complement(3036..3791)
                     /locus_tag="PLQ30_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HPW01674.1"
                     /translation="MLLDLSEREKSILELLADRNYLSVSELSSTLGVSEVTIRADLSS
                     LENRGYLMRTRGGALPSIHRSILEHQRLHIEEKQRIAKKAAGLVCDGDRIMIEAGTTT
                     AFITRYLIGRQDVQIVTNSMLAFSYARINPLLNIILTGGTFRRETESLVGPVAVQNLD
                     NFNARLAFVGTDGFTIQRGMTTQFTEGAEIVRAMNKRAETTWLVADSSKFGKIGFVSV
                     LPLLAVHGIITDGGLPKEALEALQTEGIEVILA"
     gene            4137..5657
                     /gene="gguA"
                     /locus_tag="PLQ30_04185"
     CDS             4137..5657
                     /gene="gguA"
                     /locus_tag="PLQ30_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPW01675.1"
                     /translation="MATKLLSMEKIDKRFTGVHALRQVDFDLDEGEIHALVGENGAGK
                     STLMKVLVGINPKDSGQIYYMGHLFNPRDPRHALEMGIGIIHQELNMMEHLTVAENIF
                     IGREFTRRNGIILDKREQNKRAEELFTRLKMDIDPTEKVGRLTVGKQQMVEIAKAVSH
                     RLRILILDEPTAALTDAEIEELFAIMRDLKNQGVGMIHISHRLEEIHQIADRVTVMRD
                     GEIVSTKKTNDVTRNQVINMMVGRVIYEQPKVKSMVAPGAPVVLRTHRLNAGKLVHNV
                     SFELRKGEILGFAGLIGAGRTETARAIFGADPIQSGWIEVNGRRVRINSPQDAVAKGI
                     GYLSEDRKRFGLAVNLCLRDNIVMASYDQFTRGLFVDAKKAEDVTEHYIERLRIKTPS
                     VDQLIRNLSGGNQQKVVIAKWLICNCEILIFDEPTRGIDVGAKSEIYAFMNELTREGK
                     SIIMISSELPEILRMSDRIIVMCEGRITGELPIEEANQEAIMHLATMGRQAVLARE"
     gene            5662..6942
                     /locus_tag="PLQ30_04190"
     CDS             5662..6942
                     /locus_tag="PLQ30_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW01676.1"
                     /translation="MALETVEESRMQRFKERGLKYALAPAALIILYVFFGIFGRNYFS
                     YQTLVNIIDAAYYIGFISMGVTFVIISGGIDLSVGTVMMASTLIGGVALKNGVPMVPS
                     LLIIILVGTAFGFFNGIIVSRLRLPPFIATLGTMMISMGVGSIVSNVQSATFPIRGQP
                     GGWYKNLFLYLSPTGGMIPTGAILLLLVAVICHLILSNTKLGRYTYAIGSNIEAARLS
                     GVDVQKWQMWVYVIAGVTAGIGGISYAAIYTTILPAQGQGFELYAIAAAVIGGTSLSG
                     GVGTIWGTIIGVFIMSVLSTGLPAMNLQAHWQTFFTGVIVIGAVLLDMYRTRKAAEIK
                     IETAADIYKAEMLEKIASMQRELSALKASGDTAKAAELQEKIAATRREMKATYATKKA
                     EEKAELARIRAEERAADHEFTEMLKQTDHILDKK"
     gene            7027..8115
                     /locus_tag="PLQ30_04195"
     CDS             7027..8115
                     /locus_tag="PLQ30_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW01677.1"
                     /translation="MHKTRRVGTVIVTLILLAAITVPVFAAGPIFIPMISKGFQHQFW
                     QTVMKGAKDAADKYNVVMTFEGPPTEADIQQQVQMLVNAMAKSPSAICLAALDTNSVM
                     DQLNEAMRRKIPIIGFDSGVPNAPKGAIYATAATNSYAAAGLAAQKMFPVIKDKIKAA
                     TPSNPITIIDFNQDATSQSVTDRGKGFRDMMIKLITTEGGRSLSDIKVTGNRAYIAPD
                     SPTSGKAIILDMVVPASPKDTDATATAQSILNRVKSDHIVGIFCSNEGTARALLSASN
                     DGAALPTQYPGLVVIGFDAGKAQKAAVRNRYFFGAITQDPYMIGYYAMELAYKAVMGQ
                     PVADIDTGAKFFDYSNMDQPDIAQLLYD"
     gene            8294..9286
                     /locus_tag="PLQ30_04200"
     CDS             8294..9286
                     /locus_tag="PLQ30_04200"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HPW01678.1"
                     /translation="MIAACGESLIDMVPSEQGADLFEACPGGCPYNSAIAAARLCVPT
                     WFIGKTSIDFLGDKIIDKLKSSGVDLSMLSRSEQAVTLAFVERDAGGNAKYAFYSEGA
                     ADRFYYPSDLPVTLPESCRFLLVGSISLAQEPSGSSICSLIEREHIARLVSFDPNIRP
                     NLISDKVRYIERFEWICQRSAIVKASDSDLEWLYEMPGRKAAQKVLEMGPELVAFTLG
                     DRGAVLMTKARQVEMPAKSVPVVDTIGAGDSFHAALLVGLGWLKVSTRKDLSALSEEA
                     MQSILRLAISEAALDCTKRGAEPPTLRELALFDPEAVKIAPGIMSSFNTRGVIC"
     gene            9280..9717
                     /gene="fucU"
                     /locus_tag="PLQ30_04205"
     CDS             9280..9717
                     /gene="fucU"
                     /locus_tag="PLQ30_04205"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HPW01679.1"
                     /translation="MLIGIKPCISAELLSVLYRMGHGDELVLADAFFTGDSLNSRVVR
                     ADGIRIPDLLDGILRLINLDTYVDDPVVMMLPSKGDLLDPEVEQRYREVIDRYWPKTP
                     PIARMERFAFYERARKAFAVVMTGETVKYGNIILKKGVPPVSD"
     gene            9772..10803
                     /locus_tag="PLQ30_04210"
     CDS             9772..10803
                     /locus_tag="PLQ30_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01680.1"
                     /translation="MEQALGSLLSIAVLMVAGYLLAKQGLLPEKAVEGLRLLITNLTL
                     PLVMFRAFLLLHLEGRNLALAVVVFFACAAMGIVGAAIGRLMHAPSPETRFLYQGFEA
                     GMLGYVLFGGFYGAEQLPAFAALDIGQVIYVFTVLMVQMNMAEQARASRIQELQEKAD
                     MALASKGHGASSQEERCKMKKRSMAGTVFPWRDIVNSKVLWAICLGVVLSIAAPEFAQ
                     RLGEKNVVMNMLFAMVGGLTTPLVCLVIGASLSGGLVLSREVVWVVATRLVVALAMGF
                     GMAHWVVPALGFGVWQRRAALIMFVLPPPFIIPVYYKGNIQFVSSVLTLFTLIAIVTV
                     ALLVLLGVV"
     gene            10803..11810
                     /locus_tag="PLQ30_04215"
     CDS             10803..11810
                     /locus_tag="PLQ30_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPW01681.1"
                     /translation="MSKATVTIRHVAERAKVSTATVSRVLNNNPCVRQRTRQTVLEAI
                     DSLGYKLNVVARSLKTSRTHTIGVLAPDLSGDFFMFIAESMNRELARRGYSLVVGTSQ
                     GSEEEEAKQVRHLAERLVDGIVLIPVGGSNAYLPQISDFNIPIVFVDRILKGYIADKV
                     LVDNEEGACKAVSALIGEGHHRIGYLGGNPEVTTAEERYKGYCRAMKEAGLEIEQKYV
                     RFGNPTLPFGYRAMEDIIKDPYSPNTWFIVNIFTHLGATNYLISEGEERTRQITFASF
                     DEMPYSPLLRYCRYAVQQPIAEMGKAAANLIIDRIEGIGPPEPQTLRFKTRLIQHKLV
                     R"
     gene            11836..13650
                     /locus_tag="PLQ30_04220"
     CDS             11836..13650
                     /locus_tag="PLQ30_04220"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HPW01682.1"
                     /translation="MEQSKDFFLQPPKNRWKESPPKIGIRPAIDGRRRGVRESLEEVT
                     MGMAKAAARLISENIRHPTGEPVECVIADTTIGGVAEAAACAEKFAREGVAVTLTVSP
                     CWCYGSETMDMDPLTIKGVWGFNGTDRPGAVYLAAVLAAHSQMGLPAFGIYGRNVQDI
                     SDAQRIPEDVAEKILRFARAGIAAAWMRGKSYLSVGSVAMGIAGSIVNPDFFREYLGM
                     RNEYVDMSEIVRRIEEKIYSEAEFRRAMQWVKTYCKEGQDNNPLDEQHSRVRKDRVWE
                     TCVKMAMIVRDLMVGNPDLRRKGREEEALGHNAILAGFQGQRQWTDHFPNGDFLETIL
                     NSSFDWNGIREPYLVATENDSLNGVSMLFGHLLTGSAQIFSDVRTYWSPEAIRRVTGW
                     QPTGLAEEGIIHLINSGATCLDGTGCQRINGQPAIKPWWQVEPEEAKACLEATQWRYA
                     SLGYFRGGGYSSDFLTEGGMPITMTRINIIKGIGPIIQIAEGHTVTLPQEVHDTLDLR
                     TDPTWPTTWFAPRLTGTGPFKDVYSVMANWGANHGASCYGHIGADLITLASMLRIPVA
                     MHNVPSEKIFRPSFWNAFGMDPEGADYRACSALGPLYR"
     gene            13986..15701
                     /locus_tag="PLQ30_04225"
     CDS             13986..15701
                     /locus_tag="PLQ30_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW01683.1"
                     /translation="MHYLSRYRKLALPGVGVMIAIAGGQLAGPYILKQIIDVAVPKGD
                     TSLLLAYGAAFLVIVSVTGALSYLGMILLARLGLSIVTHIKQDIFLHLLKLPVSYFDK
                     HPVGELMSRTETDTERVRDMFSTLGANLIVNVLTMIGIFLVTFALMPALALIMVGISL
                     GLLIILILFFSKIFPMYEKARSLYAKITAKVTEFIQGIEVLKAYGRTGWAEANLDTTA
                     KERMSLEVRISLIEYSMMSLLNALIGPMFIVVLLLLYAPKALTGAMTLGTILLFVEYG
                     ARLLRPIAEIAESLRSLQQARTSLSRIRTIMATPEEPNRGSGKAPTFEREIRFDHVWF
                     AYEDEDWVLRDVSFVIPKGSFTAVVGASGSGKSTIISIICGFAFPQRGKVLVDDIPLD
                     EIDIVAWRRHIGLVLQESFMFPVSVLENVRLYQEEITEDKVKSALSIVHADKAVSMLP
                     DGLEANLWERGSNLSSGERQLISFARALAADPDLILLDEATSNVDMTTEKHIKESMDV
                     LRKGRTMVVVAHRLSSILKADRIFYIDEGKLITEGTHESLYEELPAYRLLVDQQFPAG
                     KRVLV"
     gene            15698..17575
                     /locus_tag="PLQ30_04230"
     CDS             15698..17575
                     /locus_tag="PLQ30_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW01684.1"
                     /translation="MNQKLYHLRWIREVWRERQALVWLLLFLTLVSSAVAVAFPLLTK
                     QLFDLLEHAIDTKMSEDVAMAQVKRIALYFAAIGVAGLIAGVFPGVRGALNMVFDYIV
                     RKRYFGEVVRKDIRFFSEFRSGDVITRLTSDISDFPKISWFLCSGIFRAVESASKVTF
                     CIIAMFSLDWRLALASLASLPVMLFVFSRTQAAIYDRVNKNEQAISEINNQLEMSFSG
                     VRVIKAFASEEKYTSFFEDALKKRFNTEMAVIKLETVMHLIYQYIDYVAQIALVFVGG
                     LMVTKRSISIGTFYAFYNYLNMLIYPVLDIPQLFVFGKRAFVNIDRLEEIRQYETGSQ
                     KQCATSTQKKYDPSIQNQNKASAQRQEKSTPKKDIASDKAFRSLCFENVTLRYEQRRK
                     AALSDISLEILPGEKFLIIGPVGSGKSTLIKTALGLLEPQKGRVMINNKPVRSLDPTE
                     IRGLIGYVPQDPLLFSGSIRENILFGCREGQNLTDEELFRIIEVSRLTEEIEYFSDGL
                     ETKLGQRGTSVSGGQKQRIAIARALAGYPALLLLDDMTASLDTNNEEALWHSLAAMPE
                     FAQAAVIAVSHRLSSIQYVDRVLFLNGGRIAGFGKHTELMEQNGLYREFVAEHVRRPT
                     G"
BASE COUNT         4560 a         4063 c         4571 g         4720 t
ORIGIN      
        1 tatttcgctt gtactgattg atcggtatag aagggatttc gagcatccca ggtttcctga
       61 cgtcttcagg tttgatgggc aatgattttg gcatattatg actccttatg ggctcattcc
      121 gagccaattt tcagttagtg tatatgtgtt ccgctgagcg gatttgcgct ccaagctgcc
      181 tcgcggatga cttccgagac ggttggatgc gggaatacca tgttccgaaa agcagaaagc
      241 ggtagtcttc gttcgagcgc tagcgccgct ccccaaatct gctcggaagc gtaggctccc
      301 agcagatgaa ttccaagaag cctttcgctt gatttctcta tgataatctt tatagatcca
      361 ggagcagaaa aaccattttc ggctacaaat cggccagaga cgttatatgg cattcgaatg
      421 acatgggtat catatcctct tgcgcgagca tcctgttcgg ttagcccggc acctgcagct
      481 tcaggcagcg agtaaagagc ccatggcact gtttccggca cgaagatctg attgctgcgc
      541 tccgaatttt gacttgcaag gatgtcagag acagccactt ccgccatgcg gtaagccgca
      601 tgggctaact ggctctttcc tgtaacgtcg ccaatcgccc agactcctgg tagattagtt
      661 cgcatcctgt cgtctgtggc tatagcgcga ttcttaatct cgagtcctga ttcctgagca
      721 ccccaatcct ctatattggc tttgcgtcca acggagagca aaatgatatc agcttctgta
      781 gagccttttt cgccattttt gttctcaaag agtacctttt tgccctcgag ctccgttacc
      841 tttgtgccaa gcatgaaagt cacatttttt agtgcttttc tcagctcgac agccatctga
      901 ctatccatta ccggcacgat ttcgtccatc atttcgatga cggttacact cgagccaaac
      961 gcagagaaca agctggcgaa ctcaattcct attacgcctc caccaattat gcatagactt
     1021 gatggaattt cgccaattga taacaaccct gttgaatcga gtacagccgg attatctttt
     1081 atgcctctaa tcggaggaat tatcggagaa gagcctgttg caatgatgat tttctggcct
     1141 ctgatcatga aggcagaatt tgaggattgg gaatctggag attgataatc tgaggattga
     1201 gaagtctgtg cttgatataa agagggttga agcgcatcgc tttctgcgtt aaccggaaag
     1261 acgataacag aatgaccatc tgtcaacttt ccaactccac ggatgatctc tacgccggct
     1321 ttcttgaggg tcaattccaa ggatcttcgc agtctcttcg agacttcttc tttccaggcc
     1381 atgacctgcc gccagtttat atgtacacct tcggtttcta taccaaatga agaagcgtct
     1441 ctcaaatgag catatagttt ggcgctattg aggagagtct tggtgggaat acaacctata
     1501 ttgaggcaag tcccgcccac agcggttttt tccacaaggg cgactctgat tttattggcc
     1561 gcaaggcgtt ccgcagcaag atagcccccg gggcccgatc cgaggatgat cacatcataa
     1621 tcgctcatac attttctcca tagaagtcat gcatatatta aaagctatat tcgaaacgat
     1681 attcaaagcg atattcaaag agaaaagggc ataatatcga tgtctgcaat gatctcggaa
     1741 agccgtctta gaaaacgcgc ggcataagcc ccatctatta cctgatgatc tacggtgagt
     1801 gaaaggccta ttctcattcg attctcaatg cccttatcgg tttttaccgg agtgggtcgg
     1861 atcgtacata cgcccagtat tgcggtctcc ggagtattta caataggagt gaaccactcc
     1921 acaccgaacg cacccagatt cgtaacagtg aaggtagaac cagaaagaga ggcgggatct
     1981 gcgcggccgc tttggcaggc ttttgccagg cgcttcactt ccatggaaac gccgagaagg
     2041 gagagatctt gagcatttcg aatcaccgga accataaggc ctcgcggtgt atcgaccgca
     2101 acaccaaggt ggattggctt gaaaagtctt agctcatttc cttcaaggtg cgcattgaat
     2161 tcgggaaact cagggagaat cttgataacg gccgtaagaa caagatctcc aatagtgatg
     2221 tgtgcaatat ttggattctc ttgcgctttg agacgcgctc tcagtgcaac aaggtgctcc
     2281 gcatctgcag aactgtgctc ggtatactgg gcggtggttt ggaccgaatc gtgcatacgg
     2341 cccgcaatgc gctttcgtat gccaataagg gggatgctct ggaattcggt tttttcatga
     2401 gttggtggtt cttcttttat agtctgcgtc tgtgttgctt gcagtatttt gccaaaagct
     2461 ccctggatag ggggttcttg catagagact tcttggttgg atttctcgtc aatcgaggcc
     2521 tcgataacat ctttttcaag gattcttcca ccaggaccac tccctttatc gatagcccct
     2581 agatccacac caaggttgtg catggccatt cgggcgcgtg ggctcgaatg aggctctttg
     2641 ccattagcct cttttagcat tgtggcttcc ttttcttgct caaagacagt ggaagtgctt
     2701 gcagaaatag gaatcgatgc cgtatttgcc tgggaggagt ctgcggcatt ttcccacact
     2761 tcctcaggcg caccaactat cgcaataggc tccaaaaccg gcacatcatc gccttctctt
     2821 ttaagaagcg ctagcactgt accatcaact cccgcaggaa cttccattgt cgccttgtcg
     2881 gtttcgattt cgcataggat agtgtccttc gtaacagtgt ctcccggctt tactttccat
     2941 gatagaataa cgctcgattc aactgtgttg ccgagtttcg gcatgattat cttttccgcc
     3001 attttgctat ccctctcttt aattcttcta tttaattatg ccaaaattac ttctatgcct
     3061 tctgtctgga gcgcttccag cgcttccttt ggcaagccac cgtctgtaat gatcccatgc
     3121 accgctaaca agggcaatac acttacaaaa ccaatcttgc caaatttgga ggaatcggcg
     3181 acgagccatg ttgtttccgc tcttttgttc attgcccgga caatttctgc tccttctgtg
     3241 aattgagtag tcattcctcg ctggatcgta aagccatcag ttcctacaaa agctagacga
     3301 gcattgaaat tatccaggtt ttgtactgct acaggtccta caagagactc cgtttcgcgt
     3361 cgaaatgtgc cccctgttag tatgatattt agaagaggat ttatgcgcgc ataagagaag
     3421 gccagcatgg aattagttac aatttgtaca tcctgtcgac caatgaggta tcgggttata
     3481 aaagcggtgg tagtgccggc ttcgatcata atgcggtcgc catcgcagac gagtccagcg
     3541 gcctttttgg cgatacgctg tttttcttct atatgaagtc tttgatgttc gaggatgctc
     3601 cgatgtatcg aaggaagtgc gcctcctcga gttctcataa ggtaacctcg gttttcaaga
     3661 gaagataggt ctgcacgtat agtgacttcc gaaacaccca gagtgcttga aagctcggag
     3721 acagaaagat aattgcgatc agccagaagt tccaaaatcg acttctctcg ctcgctaaga
     3781 tctagaagca tttcaaacct ccggaaaact taaaaatgcc tttctaatat ctttcgataa
     3841 tatttcgtaa agctttcttt taatatatag ctagtctcca aaacatgcaa gacccttaat
     3901 tgaataaatt atgtaatcga ttgcatttta tcgcttgaca gaatacaaaa accatgtaat
     3961 cgtttactta tcgctgttga gtcggaaaga gcgcctggca gacaaggcac atcgatagat
     4021 tttatgggtt gcaagactga gtgcacggtt gtgcatcaat gtgcatgatt tagacgagaa
     4081 tatggcgata cgtgtatgac cattaagacc taaagcaggg gaaaggagtc gcagcggtgg
     4141 ccactaaact attgtcgatg gagaaaatcg ataagcgctt tacaggcgta catgcgcttc
     4201 gccaggtgga cttcgatctc gatgaagggg agatacacgc gctcgtcggg gagaatggtg
     4261 caggcaaatc tacccttatg aaagttcttg tcggcattaa tccaaaggac tcaggtcaaa
     4321 tctactacat ggggcatctt ttcaatccgc gcgatccaag gcatgcgctc gaaatgggca
     4381 tcgggattat ccatcaggaa ctaaacatga tggagcatct caccgttgct gaaaacatct
     4441 ttatcggaag agagttcact cggcggaatg gcatcatcct ggataagcgc gaacagaata
     4501 agagagccga ggagctcttc actcggctca aaatggacat cgatccaaca gaaaaagtag
     4561 ggcgtctcac ggttggaaaa cagcagatgg tagaaatcgc taaggcagtg tcccatcgct
     4621 tgagaatact cattctcgat gagcccactg cggcgctcac cgatgcggag atcgaagagc
     4681 tctttgcaat catgcgagac ctcaagaacc agggcgttgg gatgatccat atttcccatc
     4741 gtttggagga gattcatcag attgccgatc gcgtaaccgt gatgagagat ggcgaaattg
     4801 ttagtacaaa aaagacgaac gatgtcacaa ggaatcaggt catcaatatg atggtggggc
     4861 gcgtgatcta tgagcagcca aaagtcaaga gcatggttgc gccaggggct cctgtcgtgt
     4921 tgcgcacaca taggctcaat gcaggcaaac tggtgcataa tgtgagcttt gagctccgaa
     4981 agggtgaaat tcttggcttt gcagggctta tcggagcagg ccgcaccgaa accgcacgcg
     5041 ctattttcgg cgctgatcct attcagagtg gttggatcga agtcaacggc agaagagtca
     5101 gaatcaattc accccaagat gcggtggcga agggcattgg gtatctttcc gaagatcgta
     5161 agcgctttgg tcttgcggtc aatctttgcc ttagagacaa catagtaatg gcatcctacg
     5221 atcaatttac acgtggtcta ttcgtcgatg ctaaaaaagc ggaggatgtg accgagcact
     5281 acatcgaaag gcttcgaatt aagacacctt ctgtcgacca gctcattcga aacctatcgg
     5341 gcggcaacca gcagaaagtc gttattgcaa aatggctcat ttgcaactgt gaaattctca
     5401 tttttgatga acccacgcgc ggaatcgacg taggagcaaa gagcgaaatc tatgctttca
     5461 tgaacgagct tacaagagaa ggaaagtcga taatcatgat ctcttcggag ttgccggaga
     5521 tcctgcgcat gagcgataga atcatcgtta tgtgcgaagg acgcataacc ggagagttgc
     5581 ccatagaaga ggctaaccag gaggctatta tgcatcttgc cacaatgggt cgccaagctg
     5641 tccttgcaag ggagtgaaca catggcttta gagactgtgg aagagagccg catgcagcgg
     5701 ttcaaggaac gaggtttgaa atatgcgctt gccccagccg cattgataat tctgtatgtg
     5761 ttctttggta ttttcggaag aaactacttc tcgtatcaga ccttggtaaa catcatcgat
     5821 gctgcctatt acattggatt catctctatg ggagtgacat ttgtcataat ttctggtggc
     5881 atagatctct ctgtaggaac ggtgatgatg gcatccacgc tcattggtgg agttgcgctc
     5941 aagaatggcg tgcctatggt cccatcgctg cttattatca ttttagtggg cactgcattt
     6001 ggattcttca atgggatcat tgtctctaga ctaaggttgc cgcctttcat tgcaaccctc
     6061 ggtacgatga tgatctctat gggcgtgggt tctatcgttt cgaatgtcca gagcgctacg
     6121 ttcccaattc gtgggcagcc cggcggttgg tacaagaacc tttttcttta tttgtcaccg
     6181 acaggcggca tgataccaac aggtgctatt cttttgcttc ttgttgctgt gatttgccat
     6241 ctcattttgt caaacacaaa acttggtcgc tatacctatg caataggtag caacatagaa
     6301 gcagcccgac tttctggcgt agatgtgcag aagtggcaga tgtgggttta tgtcattgcg
     6361 ggggtaactg caggaattgg tggcatttcc tacgcagcaa tctatacgac tattctccct
     6421 gcgcaagggc agggttttga gctttatgct attgccgcgg cagttatcgg tggtacctcg
     6481 ctttccggag gcgtcggtac tatttggggg accataatcg gcgtattcat catgtctgtg
     6541 ctttcgacgg gcttgccagc tatgaatctt caagcccatt ggcagacctt cttcactggc
     6601 gtgattgtta taggtgctgt gctcctcgat atgtatcgaa caaggaaggc ggcggagata
     6661 aagattgaaa ctgcggccga tatctataag gcagagatgc tggagaagat cgcctccatg
     6721 cagcgcgagc tttctgcatt gaaagcgtct ggcgacacag caaaggccgc tgaacttcaa
     6781 gagaaaatcg cagctacccg acgcgaaatg aaagcaacct atgcgacaaa gaaagcagaa
     6841 gaaaaagctg agctcgccag gattcgtgcc gaagagcggg ctgcggacca tgaattcaca
     6901 gagatgctca agcaaacgga tcatatattg gataagaaat agaatcgcaa tggagtccgc
     6961 gttgcgggct taattggcag ataagcttcg gagacaatcc ggagcattaa tgtagaggag
     7021 gtaggtatgc ataagacaag aagagttgga acagttattg tgacgctcat tctcctggct
     7081 gcaattactg ttcctgtgtt tgctgcaggc ccgattttta tccccatgat ctccaagggc
     7141 tttcagcatc agttctggca gacggtcatg aagggagcga aagacgccgc cgataaatat
     7201 aatgtggtaa tgaccttcga aggaccaccg acagaagctg atatccagca gcaggttcag
     7261 atgctcgtca atgcgatggc caagagccca tccgccatct gcctagcagc tctggacacc
     7321 aattccgtta tggatcagct caatgaagcg atgaggcgca aaatcccgat cattggattt
     7381 gactcgggcg tacccaatgc gcccaaggga gccatctatg caaccgctgc tacaaacagc
     7441 tatgctgctg cagggcttgc cgctcagaag atgttccctg tcatcaagga caaaataaaa
     7501 gcagcaactc cgtcgaaccc catcacgatc atcgacttca accaggatgc gaccagccag
     7561 tccgtgactg accgtggcaa aggattccgc gacatgatga tcaagctcat caccaccgaa
     7621 ggtgggcgct cgctcagcga catcaaggtg actggcaacc gggcatacat tgcacccgac
     7681 agccccacca gcgggaaagc gataatcctg gacatggtag ttcctgcttc gccaaaagac
     7741 accgatgcaa cggccactgc tcaatcgatc ctcaatagag taaagagcga ccacattgtg
     7801 gggattttct gctcgaacga aggaacggct cgcgccttac tttctgcatc caatgatggc
     7861 gcagcgctgc caactcagta tccaggcctg gtggtgatcg gattcgatgc tggcaaagcg
     7921 cagaaagccg cggttcgcaa tcggtatttc tttggagcga tcacgcagga tccctacatg
     7981 atcggctatt atgctatgga actcgcctat aaggcggtga tgggccagcc tgttgcggat
     8041 atcgatactg gtgcgaaatt tttcgactac agcaacatgg accagccaga catagcgcag
     8101 cttttgtatg actgaaaaat cttctgagtt ctaagaagat ctgtctggtt taagggtatt
     8161 acatggcgta gaaaggggct gctcattctt ttttgggcag ccccttttgc ctaataattg
     8221 aatcaattag taagagttaa gaggaagaga aaaggcaggg tatgtgtctg cttttcttga
     8281 ggagcaacgg agtatgatag cagcgtgtgg cgaaagtctg atcgatatgg tgccctcgga
     8341 gcaaggcgca gatctttttg aagcatgccc cggtggctgc ccatataata gcgctattgc
     8401 ggctgcgcgc ctctgtgtac caacctggtt tatcggcaag acttcgatag actttttggg
     8461 agataaaata atcgacaagc tgaaaagttc gggtgtagat ttgtcgatgc tttcacgtag
     8521 cgagcaggcg gttacacttg catttgtcga gcgagatgct gggggtaatg caaaatatgc
     8581 gttctattcg gaaggcgctg ccgatcgttt ttattacccg agcgatttgc ctgtaacgct
     8641 tcctgaatca tgccgatttt tgctagttgg ctccatttct cttgcgcagg agccttccgg
     8701 ctcgagcatc tgctcgctta ttgaaagaga acatattgcg aggcttgtga gcttcgatcc
     8761 gaatattagg cctaacctta tttccgataa ggttcgctat attgagagat ttgagtggat
     8821 atgccaaaga agcgctatag tcaaagcgag cgactcagat ctcgaatggc tctatgagat
     8881 gccggggcgc aaggctgctc aaaaagtgct ggagatgggc cctgagctcg ttgcgttcac
     8941 tttgggcgat cgcggtgcag tccttatgac taaggcgaga caggtcgaga tgccagcaaa
     9001 atctgtgcct gttgtcgata cgatcggtgc aggtgacagc tttcatgccg cgttgcttgt
     9061 gggcttgggt tggctcaaag tcagcactcg gaaggatctg agtgcactca gcgaagaggc
     9121 catgcaatcg atattgcgcc ttgccatttc ggaagcagcg ctggattgta ctaagcgcgg
     9181 tgcagaacca cctacgcttc gcgagctcgc tttgttcgat cctgaagcag tgaaaattgc
     9241 tcccggaatc atgtcgagct ttaacacgag gggagtcata tgctgattgg aatcaagcct
     9301 tgtatctccg cagaattatt atcggtgctc tataggatgg ggcatggcga cgaattagtc
     9361 ctcgccgatg cattttttac tggagattcg ctcaactcgc gcgtggtacg agctgacgga
     9421 attcggattc cagatttgct cgatggaatt ctgcgtctta tcaacctgga tacatacgtc
     9481 gatgaccctg tggtgatgat gcttccatca aaaggtgatt tactcgaccc agaagtggaa
     9541 cagcgctatc gggaggtgat cgatcgctac tggcctaaga cgccacccat cgcgcgaatg
     9601 gagcgatttg ccttctacga aagagcgcgc aaggcctttg ctgtcgtcat gacaggagag
     9661 acagtcaaat atggcaatat catccttaaa aaaggagtgc caccagtctc cgattgagag
     9721 cttgtgtata acagtctcca aagaacaatc tccgaatgag gattgataaa gatggaacag
     9781 gcgcttggtt cgctactttc aattgctgtg ctcatggttg caggttatct acttgcaaag
     9841 caggggctgt taccagaaaa agcagtggaa ggtttgcggc tactcatcac taatctcacc
     9901 ctgccgcttg tgatgtttcg agcttttcta ttgctacatc tggagggaag gaatcttgca
     9961 ctggccgttg tagtattttt tgcatgtgcg gcgatgggaa tagtcggtgc agcgataggg
    10021 cgccttatgc atgcgccaag cccggagacg aggtttcttt accagggatt tgaagcgggg
    10081 atgcttggct atgtgctgtt tggcggcttt tatggagccg agcaattgcc tgcatttgcg
    10141 gcactcgata tagggcaggt aatctatgtg ttcaccgtgc tcatggtcca gatgaatatg
    10201 gcagagcaag caagggcaag ccgaatccag gaattacaag aaaaggctga tatggccctt
    10261 gcttcgaaag gacatggcgc atctagccaa gaagagcgct gtaagatgaa aaagaggagc
    10321 atggcaggga ctgtctttcc atggcgagat atcgtgaact caaaggtgct atgggcaatt
    10381 tgccttggtg tggtgctttc gattgcagcg ccagaattcg cacagaggct cggggaaaag
    10441 aatgtagtga tgaacatgct ttttgcaatg gtaggggggc ttaccacacc actcgtgtgt
    10501 cttgtaattg gcgcatcgct cagcggaggg cttgtgctta gccgcgaggt ggtttgggtg
    10561 gtagcgacgc gccttgttgt agcgctggct atggggtttg gaatggcaca ttgggttgtg
    10621 ccagcgctgg ggtttggggt gtggcagaga cgcgcggctt tgattatgtt tgtgctaccg
    10681 ccgcccttta taattcccgt atattataag ggaaatattc agtttgtaag cagcgtactc
    10741 acgcttttta cgcttatagc gattgtgact gtggcacttt tggtgttgct tggagtcgta
    10801 taatgagtaa ggctaccgtg acaattcggc atgttgcaga gagagcgaag gtttcgactg
    10861 caacggtgtc gagagtgctc aacaataatc cttgtgtgcg tcaaaggacg cgccagacag
    10921 tgcttgaagc gatagactca cttggctata aactcaatgt tgtcgcgcgt agcctcaaga
    10981 caagtcgcac tcacactatt ggagtgcttg cgcccgacct ctctggcgat tttttcatgt
    11041 ttattgcaga gagcatgaac agggagcttg cgaggcgggg atattctctg gttgttggca
    11101 ccagtcaagg ttcagaagaa gaagaagcaa agcaggtgcg tcatctcgcg gagcggctag
    11161 tcgatggaat agtacttata cccgttgggg gttccaacgc ttatcttcct cagatttcag
    11221 acttcaatat tccgattgtc tttgttgacc gaatactcaa aggctacata gcagacaagg
    11281 tgctggtcga caatgaagag ggagcatgca aggcggtcag tgctctgata ggagaagggc
    11341 atcatcgcat tgggtatttg gggggaaatc ccgaagtcac tacggcagaa gagcgctata
    11401 aaggctactg cagggctatg aaggaagccg gactggaaat cgaacagaaa tatgtgcgct
    11461 ttggaaatcc gacgcttcct tttggttatc gggctatgga agacataata aaagacccct
    11521 attcgcctaa cacctggttt attgtcaaca tttttacgca tctaggtgca accaattact
    11581 tgatttctga gggagaagag aggactagac agatcacctt tgcatctttc gatgagatgc
    11641 catattcacc cctattgcgc tattgtcggt atgcagttca gcaaccgatt gcagagatgg
    11701 gaaaagcggc agccaatctc atcatcgata gaattgaagg cataggacca cctgaacccc
    11761 agacgctccg gtttaagaca agattgattc agcataaact tgtgcgctaa acaacatatt
    11821 cgttggagga actgtatgga acagtcaaaa gatttttttc ttcagccacc caagaatagg
    11881 tggaaggaga gtccaccaaa gataggtatt cgccccgcca ttgatggccg tcgcaggggt
    11941 gttcgcgagt cactggaaga ggtgacgatg ggcatggcca aggcagcggc gagactaatc
    12001 tctgagaata ttcgccatcc tactggtgag cctgttgaat gcgtaatcgc agatactacg
    12061 atcggaggcg ttgccgaagc agccgcttgt gctgagaaat ttgccagaga aggagtggcc
    12121 gttaccctta cagttagccc ttgttggtgc tatggctctg agaccatgga catggatcct
    12181 ctgacaatca agggagtgtg gggattcaac ggcacggatc ggccaggagc ggtatatctt
    12241 gcggcggtat tggcagctca ttctcagatg ggcttgccag cttttggcat ctatggtcgg
    12301 aatgtccagg acatttcgga tgctcaaaga attccagagg atgttgcaga gaagattctg
    12361 cgcttcgctc gcgcgggtat cgctgctgca tggatgcgtg gaaagagcta cctctccgta
    12421 ggctccgtag ccatgggtat agcaggctcg atcgtcaacc ccgatttctt tcgagaatac
    12481 cttggtatgc gcaacgaata tgtcgatatg agcgaaattg tgcggcgtat cgaagaaaaa
    12541 atctattcgg aagcagaatt tcgtcgggca atgcaatggg taaagaccta ttgcaaagaa
    12601 ggtcaagaca acaatcctct cgatgaacag cattctcgag tccgcaaaga cagggtatgg
    12661 gaaacctgcg tcaaaatggc gatgatcgtg cgcgacctca tggttggcaa tccagatctc
    12721 cgtagaaagg gaagagaaga ggaagctctc ggtcataatg cgattctagc gggcttccaa
    12781 gggcagcgcc aatggaccga tcacttcccc aacggggatt ttctcgagac aatactcaat
    12841 tctagtttcg attggaatgg aattcgcgaa ccttatctgg tcgccaccga gaatgattcg
    12901 ctcaatggag tttcgatgct ttttgggcat cttttgaccg gtagtgccca gatcttctcc
    12961 gatgtacgaa catattggag ccccgaagca atacggcgtg ttacgggttg gcagcccact
    13021 ggtctggcag aggaaggcat tatacacctc atcaattccg gcgcaacctg tctcgatggc
    13081 accggctgtc aacgaatcaa tggtcagccc gcaataaaac catggtggca agtagagccg
    13141 gaagaagcaa aggcatgtct cgaagcgaca cagtggcgct atgcgagcct tggatatttc
    13201 cgaggaggcg gatattcttc agactttttg accgaaggcg gaatgcctat caccatgact
    13261 cgcatcaata ttatcaaggg tattggccct ataatccaaa ttgcagaagg ccacaccgta
    13321 acgctaccgc aagaagtcca tgataccctg gacttgcgca ccgatcctac ctggccaact
    13381 acatggttcg caccgaggct cacgggaacc ggtcccttca aggatgtcta ttcggtcatg
    13441 gcgaactggg gagccaacca tggcgcgagc tgttatggtc atatcggtgc ggatctcatt
    13501 actctcgctt cgatgttgcg cattccggtt gccatgcaca atgttccgtc cgaaaagata
    13561 ttcagaccaa gcttctggaa cgcctttggt atggatcccg agggagcgga ctatcgcgct
    13621 tgctcagcgc tcgggccttt gtatagatag actaatgaag agcattccat tcttttacta
    13681 gctatcagta ttatgaaggt gtaaggcatt ttataaggga aaaaactaat acaagatgcc
    13741 gggtctacta gtcgtggtcc cggcaccttt cttgtcaatt cgttcaaata tcgattcgat
    13801 tgcctggaag cccatcttga gctaggttac gattgaagta tacaattaag cgttttcgag
    13861 ctgtcctgaa atatgagcta cggctttgac tcatcggggc tatccgaaag caagagaccc
    13921 tctgaaggtt gctaattcat tatatgcaaa aaaaattgaa ttcaaatgat tatctaagca
    13981 ccattatgca ttacctgtcg cgctatagaa agctagcgct tcctggcgtt ggcgtcatga
    14041 tcgccattgc gggagggcag ctcgctggac catatattct caaacagata atcgatgttg
    14101 cggtaccaaa gggcgacact tcgcttcttt tggcttatgg ggctgcgttc cttgttatag
    14161 tgtctgtaac aggagcgttg agctatctgg gcatgatatt gcttgctcgg ctcggactat
    14221 cgattgtaac tcatataaag caagatattt ttttacacct tttaaagctt ccggtgtctt
    14281 atttcgataa gcatcctgtc ggagagctta tgtctcgcac ggagacagac accgaaaggg
    14341 tgcgggatat gttttcgact cttggcgcaa acctcattgt aaatgtactt accatgatcg
    14401 ggattttctt ggtgaccttt gccttgatgc ctgcgctcgc acttatcatg gtgggcattt
    14461 cgttgggtct cctgataatt ttaattctgt ttttctcgaa gatctttccg atgtatgaaa
    14521 aggcccgttc gctatatgca aagatcaccg ccaaggttac agagttcatc cagggtatcg
    14581 aagtcttaaa agcctatgga agaacaggat gggccgaagc caatctggat acaacggcta
    14641 aagagagaat gtcgcttgag gtgcgcattt cgctgatcga atactctatg atgtctctcc
    14701 tgaatgcctt gattggccca atgttcattg ttgtgcttct tctcctctac gctccaaagg
    14761 ctcttacagg cgctatgaca ctaggcacta tactcctctt tgtcgaatat ggagctaggc
    14821 ttctgcgtcc cattgctgaa attgccgaaa gccttagaag tcttcagcaa gcccgcactt
    14881 cgctctctag gattcgtact ataatggcta caccggaaga gccaaatcga ggctctggga
    14941 aagctccaac tttcgagcga gagattcgct tcgatcatgt ttggtttgct tacgaggatg
    15001 aggactgggt gctacgcgat gtttcgtttg tcattccaaa gggaagcttt accgcggttg
    15061 taggtgcgag cggctcgggt aaatctacaa taataagcat tatctgcggt tttgcctttc
    15121 ctcagagagg caaggtgttg gtcgatgata ttcctctcga cgaaatcgac attgtagctt
    15181 ggcggagaca tataggactt gtgttgcagg aatcctttat gtttccagtt tctgttctag
    15241 aaaatgtaag gttataccaa gaagaaatca cggaagacaa agttaaatcg gcgctttcga
    15301 ttgtgcatgc tgacaaggct gtctctatgc ttcccgatgg gctcgaggcg aatctctggg
    15361 agcgaggttc gaatctctca tccggagagc ggcaattgat ttcttttgca agagcgcttg
    15421 cggctgaccc cgatctcatc ctcctagacg aggccaccag caatgtagac atgaccacgg
    15481 agaagcatat caaagagagc atggatgtac ttcgaaaggg aaggacgatg gtagtggttg
    15541 cccataggct ttcttcgatt ctgaaagccg accgaatctt ctatatagat gaaggcaagc
    15601 tcataaccga aggaactcat gaaagcctct acgaagaatt gccggcctat cgacttcttg
    15661 tggatcagca gtttccagca ggcaaacggg tgcttgtatg aaccagaaat tatatcatct
    15721 tcgctggata cgtgaggtct ggagagaacg ccaagctctt gtgtggctct tgctattctt
    15781 gacacttgta tcgagtgcgg ttgctgtggc ttttcctctg cttacaaagc aactctttga
    15841 tttacttgaa catgcaatag atacaaaaat gagcgaagat gttgcaatgg ctcaagtgaa
    15901 acgcatagcg ctctattttg cagcaatagg cgtagcgggg cttattgcgg gcgtattccc
    15961 tggtgtacgc ggagcgctca atatggtttt cgactacatt gtgcgcaaaa ggtatttcgg
    16021 cgaagtagtg cgaaaggaca ttcgattttt cagcgaattc cgatcaggtg atgtcattac
    16081 gcgcttgaca tctgacattt cagattttcc caagatctcc tggtttttat gttccggcat
    16141 ttttcgagcc gtcgagtctg caagcaaggt cacattttgc atcattgcaa tgttcagtct
    16201 cgattggaga ctcgctctcg catctcttgc ttccctgcct gtaatgcttt ttgtgtttag
    16261 ccggacacag gcggcgatct atgatcgtgt gaataaaaat gagcaagcca tatctgagat
    16321 aaacaaccaa cttgaaatga gtttttcggg agtacgtgta ataaaggcct ttgcaagtga
    16381 agaaaaatac acaagtttct tcgaggatgc tcttaaaaag cgattcaata cggagatggc
    16441 tgtcataaag ctggagacag ttatgcacct catctatcag tatatcgact atgtggcaca
    16501 gattgctctt gtgttcgtgg gcggattgat ggtgacaaag cgaagtatct cgataggaac
    16561 tttttacgct ttctataatt atctcaatat gctcatttat cctgtgctgg atatcccaca
    16621 gctctttgtg tttggtaagc gagcatttgt caatatagat aggcttgaag agattagaca
    16681 atatgagact ggatctcaaa agcaatgtgc aacatcgact cagaaaaaat atgacccgtc
    16741 gattcagaat caaaataaag catctgctca gagacaggaa aaatctacac caaaaaaaga
    16801 tatcgcaagc gataaggctt ttcgctccct ttgtttcgaa aatgtcacat tgcgatatga
    16861 gcaacggcgc aaagcagctc tatcggatat cagcctggag atattgccgg gcgaaaaatt
    16921 ccttataata ggaccggttg gatcaggaaa atccaccctt attaagaccg cactaggttt
    16981 actcgaacct cagaagggac gagtaatgat aaataataag ccggtgcgca gcctggatcc
    17041 gacagagatt cgcggcctta taggatatgt gccacaagac cctctcttgt ttagcggctc
    17101 gatacgagag aatatcctgt ttggttgtcg agaagggcag aaccttactg acgaagagct
    17161 ctttcgtatc atcgaagtat ctcgtctcac agaagaaatc gaatattttt cagacggatt
    17221 agaaaccaag ctcggacagc gcggcacaag cgtttcagga gggcagaaac agcgtatagc
    17281 aatagcccga gcgctcgcag gctatccagc tctgctctta ctcgacgata tgaccgcctc
    17341 cctcgatacc aataacgaag aagccttatg gcattcgcta gccgcaatgc cagaattcgc
    17401 acaggctgcg gttatagcag tatcccatag gttatcaagc attcaatatg tcgatcgagt
    17461 gctcttcttg aatggtgggc gcattgctgg ttttggcaaa cacacagagc ttatggaaca
    17521 aaacgggctc tatcgtgaat ttgtggcgga gcatgttaga agaccaactg gctaaatcag
    17581 cttagcaagc ccacaatctc gattttggag caagaattat cgtccttatt ctatggttag
    17641 atgatgtgcg acaattctat gcagaagtag ccaggcagca tatcttcgag cccaataatc
    17701 ctcttatttc ttgactggtc ttgctttggc atctacaata atggaattat ggaacgcact
    17761 attcgactct taacttttat tttaatgatt ttggtggttg gtagcgtcga tgctcaacag
    17821 cgcattgtct tagcagggcg cggagccaat atgcttgcgg atgccattta tctttttcct
    17881 gacacaggag agagacttgt agcatattca agcg
//
LOCUS       DAOMZL010000033        17890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_2793_length_17890_cov_21.838968, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000033 DAOMZL010000000
VERSION     DAOMZL010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 17890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17890
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2793_length_17890_cov_21.838968"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /gene="carB"
                     /locus_tag="PLQ30_04235"
     CDS             complement(<1..1632)
                     /gene="carB"
                     /locus_tag="PLQ30_04235"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPW01685.1"
                     /translation="MKKVLVLGSGGLKIGQAGEFDYSGSQALKALREENIRTILINPN
                     IATNQTSEGMADTTYFLPLTPEFVTQVIEKERPDGILLSFGGQTALNCGLALCKAGVL
                     YKYAVDVLGTPIEAIELTEDRELFANHLRKLGLKTPESKAAANIVEALTCANTIGYPV
                     MLRAGFALGGAGSGLCKNERELRIRAEEALALSPQLLIEEWLGGWKEIEYEIVRDKTD
                     NCIAICNMENIDPLGIHTGESIVVAPSQTLTDEEFHNLRSIALKLARSLGIVGECNIQ
                     YALDPRCGDYRIIEVNARLSRSSALASKATGYPLAYVAAKLALGYSLIDLHNRITGVT
                     TAFFEPALDYCVIKMPRWDLAKFKEVDTHIGSEMKSVGEVMAIGRSFEEALQKAIRMT
                     GASQFGLQSDGGRMPIEPKKLRASLSKPTDKRLFAVYQALAEGWSIQKINRLTGIDPW
                     FIEKIDHIRQCEVEMYMSAESACQAQVAPSVPILDDKTIAVLLDAKRLGFSDAGIAHI
                     FGTNELEVRRWRQIMGLRPSVRQIDTLAAEYPAQTN"
     gene            complement(1629..2819)
                     /gene="carA"
                     /locus_tag="PLQ30_04240"
     CDS             complement(1629..2819)
                     /gene="carA"
                     /locus_tag="PLQ30_04240"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HPW01686.1"
                     /translation="MSVHFKSACLVLEDGSTFEGYSIGADISAEGEVVFSTGMVGYNQ
                     SLTDPSYCGQILVFAYPLIGNHGVPRMRKNAFGIPIDFESEKIQVSAVVIAEACSEPS
                     HYSAERSFSKWLNDENVPGIAGIDTRRLIRTLREEGVMHGKILIEGTPQPIRDEFKVG
                     NPVRLVSPSSPKRYIPENYQLTDRSSTKKWEKYGSVENSSKMSKRPTIALVDCGAKAN
                     ILRILLDAGSEVIRLPWDYPLDHIEYDGLFVSNGPGDPKACTRTIAHIRHALMESKPI
                     FGICLGTQLLALASGADTYKLKYGHRGQNQPVIETKSRRCYITSQNHGYAICEDSLPT
                     GWEPWFVNGNDETIEGIRASKAPFRAVQFHPEGCPGPRDTQFLIQEFLQEVAVASSKT
                     RYES"
     gene            complement(2953..3909)
                     /locus_tag="PLQ30_04245"
     CDS             complement(2953..3909)
                     /locus_tag="PLQ30_04245"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPW01687.1"
                     /translation="MFSALQSVFSVIIMIAIGFGLSKARWFEDGGSVLVSRLVVNVAL
                     PAYMISNLMGGYDRQTLLSMLPGLVIPFAVMIVSYLMAMMVGKLLKVKEDRKGTFRSM
                     FALSNSVFIGLPVNLLLFGDESLPYALLYYIANTTLFWTIGVYGIAMDGALRNSRPKP
                     SLVSLSGLKRILSPPLIGFLIAVLFILTGIMLPKPIMDTCKYLGNMTTPLSMLFIGIV
                     IARVDWKELKFEKDFIAVLVGRFLITPMFMFMVVRSLDIPLLMKEVFVMQASMPAMTQ
                     TPILAETYKSDTEYAAIGTSLTTVASMITIPFYMSIMRVLFD"
     gene            complement(3978..5384)
                     /locus_tag="PLQ30_04250"
     CDS             complement(3978..5384)
                     /locus_tag="PLQ30_04250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPW01688.1"
                     /translation="MEDVQHESVYVAPAHDAARIEVFRSASIPSAVASLALPTVISQL
                     VTMIYNVTDTFFVGQLGDPRKVAAVSLVMPAYTMLTAIANLFGVGGSSVISRFLGSGQ
                     PAKAKQTSSFCLLSAIFVAAIFSILAFIFQRPLLMLLGANQETYDFASNYIIWVLVIG
                     GIPTVMGMLFGHLVRSEGGAKQASIGMSFGGILNIILDPIFIFTFGLEVKGAAIATLL
                     SNCAVVCYFVVYLAYKKGKTIISFVPRDLNQEFSIAAKVVSVGLPASLQNILSLTSNT
                     VLNNLTAKYGSAALAAIGIAKKIDMLPMNVTMGISQGVLPFIGYNYGAKRFDRVHQAN
                     KFARTVAVCFSLLCIIIFEIFAEPIVRLFINDPETIRLGANFLRILCLMTPMMAISYL
                     ITTMFQATGQGKRALTISVFRKTTIDVPLMFLMDKLIPLYGLMTVQPIVDTLSVGLAF
                     ALYRDFSRKLAAQTDQLK"
     gene            complement(5499..6857)
                     /locus_tag="PLQ30_04255"
     CDS             complement(5499..6857)
                     /locus_tag="PLQ30_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HPW01689.1"
                     /translation="MRIVLIGAGSAQFGFGTLGDIFSSTLLKGSHIALVDIDGEALGN
                     VYKAAQAFAEANGLNFTLSAHTDRREALKGADFVIISIEVGNRFELWDQDWKIPLQYG
                     IPQVYGENGGAGGLFHALRIVPPILEICQDCAELCPDATIFNFSNPMTSITTTVLRKF
                     PGLKVIGLCHEIASLARYIPPMLGVPLEKLELRAGGLNHFSVLISARYRETGEDAYPE
                     ILAKAPAFFERMIGYSDILEYIQETGNIPKTEGETDLPDIGRTLSSRQWADRKLFRQI
                     LEHFHLLPITSDSHIGEYIPWAHSVADNKGILDFYLLYSSSLSLLQPKIELKVKERLV
                     PIIEGILQDSGYEEAAVNIMNNGLIPSLPGNVTVEVPARVHRKGIEGVVFPDYPRAFA
                     ALLRNYTGVYDLTAEAILQKSKDLVIQALLVNPVVPICEQVPELVDVMISRQAQWLGY
                     LR"
     gene            complement(7016..7873)
                     /gene="uppP"
                     /locus_tag="PLQ30_04260"
     CDS             complement(7016..7873)
                     /gene="uppP"
                     /locus_tag="PLQ30_04260"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HPW01690.1"
                     /translation="MLIIQSIILGMVQGLAEFIPISSSAHLIIIPWLFGWNNPALTSL
                     TFDVALHLGTLLAVIVFFASDWARLISAWFKSVIHFKIGEDPDRLMAWYILLACIPGG
                     VSGLLMESKINEAFHSNPIPRSSMLFMAGSIALLALLLLIADKYAQHRRSFNQIKARD
                     ALCIGLAQALAVIPGVSRSGSTITAGLALGLEREAAARFSFLLSAPIIAGAGLKSLYD
                     FSEQIGAGTIGKSELSIFPIGFIVAAVSGFFCIKYLLSYLKKHSAELFVWYRFALAAI
                     VVVVSLIRG"
     gene            8036..8512
                     /locus_tag="PLQ30_04265"
     CDS             8036..8512
                     /locus_tag="PLQ30_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPW01691.1"
                     /translation="MILQNAFKPATIKIGLESEYKDELLEELVDILAKDYPKDRIFPR
                     EEALRALRKREEKMSTGIYKGVAVPHATFESIDKLRGVIGLSKNGIDFESLDGNPVYL
                     VFMLVSPPSQAEAHLEALRQIALLIKDHLLLENLMKATTPEKVFALIREFEDSLNP"
     gene            8522..8830
                     /locus_tag="PLQ30_04270"
     CDS             8522..8830
                     /locus_tag="PLQ30_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01692.1"
                     /translation="MDSADSIDRLLEIEQKAAQIIQEAEEKASGLLVEAKKRSEIVQN
                     QKISQMRRELEADFAAYIDSLTQKSHKEIEEYRLSLNSIPLNRKSLLETLEALLQTEN
                     "
     gene            8832..9833
                     /locus_tag="PLQ30_04275"
     CDS             8832..9833
                     /locus_tag="PLQ30_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HPW01693.1"
                     /translation="MAETLDIAYLYARVCGAFSNMRLGDKAGELMQNSGGIVGLWRLL
                     FDEEAPSIAEARLVFEAERRIIKRAIDNFVRLTKPFSDSSEFIRALISKYEISTIKSM
                     LFRLRSGEPKPEATTYFSNIIGQALARWPRITDMFSDTPYSWLDSHWLDKIAMAENEL
                     DKQYYLELWKAALGLPRQKVGAIPELIRWEVIYQNIVWAIRTSRYYGLSKEDIADTLV
                     YLPQEDSTSLALETFSYDLERIGSFDSWRLKKLLSNQTGMVLDVPALEIRVLEDLFFM
                     VRRSLHLYPFSYTPIYCYFKLLDYETSLLLAVLESTRLSVTPEEKIKYVWIPGGGTA"
     gene            9830..11989
                     /locus_tag="PLQ30_04280"
     CDS             9830..11989
                     /locus_tag="PLQ30_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPW01694.1"
                     /translation="MSSVSMRKVELLLLKSDIDAVLKYLATKRCFQIIYPDEIERLAR
                     EKRASFGLGGKEELLASDNAFNKAPSTASTIARPAMNVKRFDESHLEERQINEPDLDE
                     ERLDDAQRKLDFISQFLGIEGPKDIIEDSRLPDEDMLSKLEVLFDRCSQLKVSLDEEQ
                     AKLDGLAESIHEAKAFASLSRPFEEFEKFSYVSIQIGKVAKDKIEELSQMLGDRAVII
                     PLDEEGTILAASSRKGRFAFETALSKVGFEKKKPPEGLKGIPSETMNALEGAYQVGKL
                     HFEKLLTEKKALKDQYSDLLQIIASSIRLKKAIALVESKLEKTRWAYRLSGWVPENMV
                     DKLVKDLLTMLGNRISIRVYDPPELDLAGTKEKQEGVPVLLEHNSFVSAFQGIVLSYG
                     TPLYGDIDPTPFVAFFFTLLFAIMFGDLGQGMVIASLGLAMHKAKKGLLASYKKYAHA
                     FIAAGVGSMIMGLLVGSFFTSDTALIPIERALTGFFLGKPADRFLQIMPRENVSAMFY
                     FFGFTLGVGVIINSTGIVINIINLIRRKEYGEAFFSKTGLAGALLFWWAIVMVVRIIL
                     GNKLGIVDVAGIGLPLLAIIFAEPIKSFIDKTYGKPHTEEASNWLIGGIIELLDIVSY
                     FASNTFSFLRVGAFALAHAVLSFVIFTMGSLVSGKSASGLALEIIVFLFGNAVIIVLE
                     GLIVTIQVIRLQYYEFFSKFFTRTGEPFKPVSFAGKV"
     gene            12032..12337
                     /locus_tag="PLQ30_04285"
     CDS             12032..12337
                     /locus_tag="PLQ30_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HPW01695.1"
                     /translation="MKKKLLFGVMAFMASAAVFAQTQAEPAAVSSSVQMVKYIAAALA
                     VGIAAIGGAMAVAKIGAAAMGAMAEKPDLSGKALPYVGLAEGICLWGFLVAVLILFL"
     gene            12409..12756
                     /locus_tag="PLQ30_04290"
     CDS             12409..12756
                     /locus_tag="PLQ30_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HPW01696.1"
                     /translation="MAEIFVLAEEELLIGFGMIGIKGAAIFDRDDAITAFRSVVRDKR
                     YSHEGQTFDLSDCKMLILSEDVSDMIGEELLEWQLSGDFPLIVEIPPLAGSSAQHTRL
                     VDAVRKAIGIKIE"
     gene            12779..13378
                     /locus_tag="PLQ30_04295"
     CDS             12779..13378
                     /locus_tag="PLQ30_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01697.1"
                     /translation="MEEIKGTEALEREILEDARKRAERIIRKAEESARLLGVQTEKKI
                     EEATTALVGEYQAKKRIAELEMLSRLPLEKARLDISYRDEMLRKALKGALESMNPRLF
                     GLWCVKRLACQAELVRNSRARVLVHGLDSETMRDIEALFGQGSDISIEEVPTMKARGL
                     VVEPMDTSYRISITEKELLEWLLDEKRGELAAALFGSSA"
     gene            13454..15232
                     /locus_tag="PLQ30_04300"
     CDS             13454..15232
                     /locus_tag="PLQ30_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPW01698.1"
                     /translation="MNTSIRTGVITRISGPVVLARGLEDAGLYDVVKVGESGLIGEII
                     KLYGDEATIQIYEDNTLMRVGEPVECMHRPLSVALGPGLIGSIYDGIQRPLPVLKGLS
                     GAFLKPGLAGEPLDAKKKWHFVPLLHEGDSIGQGIAYGRIQETSSIEHRLIFDARVPA
                     GIAAYVAPEGDYTVAETLVRSADGASYTSVSNWPVRKARPFREKQMTREPLVTGLRVI
                     DVLFPIAKGGCAAVPGGFGTGKTMTQHAIAKWCDADIIVYIGCGERGNEMTEVLTEFP
                     SLVDPRTGRSLMERTVLIANTSNMPVAAREVSIYTGITIAEYYRDMGYHVAVMADSTS
                     RWAEALRELSGRLEEMPAEEGFPAYLPTRLAEFYERGGMVATLEQKIGSVTIIGAVSP
                     PGGDFSEPVTQHTRRFIRCFWALDRDLANARHYPAISWNDSYSEYTDDLIAHWDAIDP
                     SWDRLRIKAMDILKKERRLSDIVRLIGPDALPDEQRLILLTADMIKNGFLQQSSFDKV
                     DMYCAPQKQTLLLQSILAFHELAENAIKNGVPLPKVSALPIREKIVRLKSSLENDKLE
                     EGRVVIQEIQAAFEQLGLNVQGAVLA"
     gene            15229..16683
                     /locus_tag="PLQ30_04305"
     CDS             15229..16683
                     /locus_tag="PLQ30_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPW01699.1"
                     /translation="MRGLAYHGIERADGPIVIAERRENIGFSELVRVKDRQGEYRLGR
                     VVELSEKAVAIQLFAENTGISIDDAWVEYLEKPLTFRVGEGIVGRIFNGLGEPIDGYP
                     PIMSSDLRDINGLPINPSARVYPRDFIQTGISAIDGMNTLIRGQKLPIFSGNGLPHNR
                     LAAQIVRQAKVLSGESQFAIVFAGMGIKYDVARFFIDSFESSGVLARVVMFLSLADSP
                     SIERLVTPRTALTAAEYLAYEKNMHVLVVMTDMTNYCEALREVSAARNEVPSRKGYPG
                     YLYSDLASIYERAGRIEGSEGSITQLPILSMPNDDISHPIPDLTGYITEGQIVLERDL
                     SQRGVYPPIAGLPSLSRLMKDGIGEGMTREDHRELAAQLFSAYAYVHTVRDLAAIIGE
                     EELSDRDKIFLNFGDHFESEFLAQGEFENRSIEQTLDLGWKMLSLLPESELVRIPPQI
                     LAKYLPHNDSDNRNDQNDQEKSHEVAILKGLKEN"
     gene            16687..17310
                     /locus_tag="PLQ30_04310"
     CDS             16687..17310
                     /locus_tag="PLQ30_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPW01700.1"
                     /translation="MAEKMPAPTKTNLIKEKRNLQLALQGYDLLEKKREILVIELMKR
                     MDSLELLEREIADMTEKAYAALRKMLLLVGRERALEISSLPVREITLSASHINVSGMR
                     LPSLDVHASNPTLNYSFMNSFAICDETVIEFTELLDKLSTAAGMRSVVWRLAREVRKT
                     QRRVNALDKMVIPRSQSIVKYIDASLDERERESLFAVKMLKRRQIGQ"
     gene            17316..17798
                     /locus_tag="PLQ30_04315"
     CDS             17316..17798
                     /locus_tag="PLQ30_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPW01701.1"
                     /translation="MKKMLGTILVAINGSDASICAFKYALALAKAFGSKIVACYVVDT
                     ATIRQLALSRIFVPEESEEYERNLENSGQRYLHFCAELAHQKQLDIETSIRRGSVSGE
                     IVKYSMEIDADIIVLGGNPVDVIYRDAIADSNHEVLKNARCPVLFVKPPIGDDLYRSI
                     "
BASE COUNT         4760 a         4048 c         4460 g         4622 t
ORIGIN      
        1 attcgtttga gcggggtatt ccgcggccag agtgtcaatt tgacgcaccg aaggccgcaa
       61 tcccataatc tggcgccaac gtcgcacttc cagctcgttg gtaccaaaaa tatgagcaat
      121 tcctgcatca gaaaagccaa ggcgctttgc gtctaagagc acagcgatgg tcttgtcgtc
      181 gagtatgggc actgacggtg cgacttgtgc ttggcaggca gactccgcag acatatacat
      241 ctcgacttcg cattgcctga tatggtctat tttttcaatg aaccatggat cgatacctgt
      301 aagtctattg attttttgta tgctccagcc ttccgcaaga gcttgataca ctgcaaaaag
      361 ccgtttgtcg gtgggcttcg agagcgaagc gcgaagtttt tttggttcga ttggcattcg
      421 accgccatcg ctctgcagac cgaattggct agcgcctgtc atgcggatgg ccttttgtag
      481 tgcttcttcg aagctgcgac ctatagccat cacttctcct acggacttca tctcgctgcc
      541 aatgtgtgta tctacttctt tgaattttgc cagatcccag cgtggcatct ttatcacaca
      601 gtaatccaag gcgggctcga agaaagccgt tgtgacacct gttatcctat tgtgcaggtc
      661 gatcaaagaa tatccgagag ctagtttagc cgctacatat gccagcgggt aaccggtggc
      721 cttggaggcg agagccgaac tgcgcgaaag tcgggcgttg acttcaatta tacgatagtc
      781 accgcatcgc ggatccaggg cgtattggat gttgcattca cctacaatac ccaagcttct
      841 agcaagcttg agagcgatgc ttcgcaaatt gtggaattct tcatcggtca aggtctgcga
      901 tggggctacg actatgctct cgccggtgtg aattcccagt ggatcgatgt tctccatgtt
      961 acaaatagca atgcagttat cagttttgtc tcgcacaatt tcgtattcga tttccttcca
     1021 cccaccaagc cattcttcga ttagcagctg cggagaaagg gcaagggctt cttctgctct
     1081 tatgcgaagc tctctttcgt ttttgcacag tccggaacct gcgccgccaa gcgcaaagcc
     1141 cgccctaagc atcacggggt aacctatggt gttggcacat gtgagcgcct ctacaatatt
     1201 ggcagctgcc ttgctctctg gcgtcttgag acccagtttc cgcagatggt ttgcaaagag
     1261 ttcgcggtct tcggtcaatt cgattgcttc aatcggcgtg ccaagtacat ctaccgcata
     1321 tttatagaga acgccggctt tgcatagagc gagcccacag ttgagtgccg tctggccacc
     1381 aaaggagagt agtatcccat caggtcgctc tttttcgatg acctgcgtta cgaattcggg
     1441 agtcaagggc aagaaatagg ttgtatctgc catgccctcg cttgtctgat tagtggcgat
     1501 attaggattg ataagtatag ttcggatgtt ctcttcgcgg agagccttga gggcttgcga
     1561 tcccgaataa tcgaattcgc cagcttgccc gattttgagt cctccggagc caagcaccag
     1621 cacctttttc atgattcata cctcgttttg ctgcttgcga cggctacttc ctgcaaaaat
     1681 tcctggatca agaattgggt atcgcggggg cctgggcaac cttctgggtg gaattgaact
     1741 gcgcgaaaag gtgcttttga ggctcgaatg ccttcgatcg tttcatcgtt tccattaacg
     1801 aaccatggtt cccatcctgt gggcagagaa tcttcgcaga tagcgtagcc atggttctgg
     1861 cttgtgatat agcagcgtcg gctttttgtc tcgattacag gctgattctg accgcggtgg
     1921 ccatatttta gcttatatgt atctgcgcca gatgcaaggg caaggagctg tgttccaagg
     1981 catattccga aaataggctt agattccata agggcatgcc tgatatgagc gatggtcctt
     2041 gtacatgcct ttggatctcc tggcccattc gagacaaaaa ggccatcgta ttcgatgtgg
     2101 tctagaggat agtcccaagg caggcggatc acttccgaac cagcatccag aagaatacgc
     2161 aggatatttg ccttagcgcc gcaatcgacc agcgcaattg taggtctttt gctcatcttt
     2221 gagctatttt ctactgagcc gtatttttcc cacttttttg tcgatgagcg atctgtgagc
     2281 tgataattct ctggtatata tctctttggg ctcgatggtg agacgagtct gactggattg
     2341 ccgaccttga attcgtctct tatgggctgc ggcgttcctt cgatgagtat cttgccatgc
     2401 atcactccct cttcgcgtaa agtgcgaatc agtcttcttg tgtcgattcc tgcaattcca
     2461 ggaacattct cgtcatttag ccattttgag aaagatcgct cagcgctgta atgactcggt
     2521 tccgaacaag cctcggctat aacaacggcg ctaacctgga ttttttcgga ctcgaaatcg
     2581 ataggaatac caaaggcgtt tttgcgcatg cgagggactc catggtttcc tatgagtggg
     2641 taggcgaata caagaatctg tccacagtat gaaggatctg tcaaagactg gttgtaaccg
     2701 accattcctg ttgaaaatac aacttcgcct tctgcagaga tatctgctcc gatcgaatat
     2761 ccctcgaacg tcgaaccatc ttcgagcaca aggcatgctg acttgaaatg taccgacata
     2821 taactcctat gcaagctttt tgcgaattta tccaaatatt ctgaatattt caagggcttt
     2881 atgcatcaaa ataggataaa tattcatata taattcatat ataatgagag gatcatagat
     2941 tcatagcaat actcaatcga agagaaccct cataatgctc atatagaagg gaatcgtgat
     3001 catcgaagcg accgtggtca acgatgttcc aatggctgcg tactcagtat cggatttata
     3061 ggtttccgca aggataggtg tctgggtcat ggcaggcatc gaggcttgca tcacaaacac
     3121 ttccttcatg agaagtggta tatctaggct cctgacaacc ataaacatga acatgggcgt
     3181 tattaggaaa cgccctacaa gcacggctat gaaatccttt tcgaacttca gctctttcca
     3241 gtctaccctc gcaatcacta tgccaatgaa tagcattgaa aggggtgttg tcatattacc
     3301 cagatactta caggtatcca tgatgggttt cgggagcatt attccggtta agataaataa
     3361 aaccgcaatc aaaaagccaa tgagcggagg tgacagtatg cgttttagcc ccgataacga
     3421 gacgagcgaa ggctttggcc tgctattgcg cagggcacca tccatagcaa tgccatatac
     3481 acctatagtc cagaagagcg tggtgttggc tatatagtat agtagggcat agggaaggct
     3541 ctcatctcca aaaagcaata gattgaccgg tagccctata aataccgaat tcgatagcgc
     3601 aaacatcgag cgaaatgtgc cttttctatc ctcttttacc ttgagcaact taccaaccat
     3661 catggccatg aggtagctta ctatcataac ggcaaaagga ataacaaggc caggcagcat
     3721 cgagaggagc gtctgcctgt catagcctcc cataagattc gatatcatat atgcaggaag
     3781 cgctacattg actacaaggc gagaaacgag gacgcttcct ccgtcttcga accatcgggc
     3841 cttcgaaagc ccaaacccga tggcaatcat tatgattacg gaaaatacac tctggagagc
     3901 actaaacatg gttgtttccc acctctctgt gccagccggt ctcttctttt ttaagaggca
     3961 ttgtatcatt atccatacta ttttagctga tccgtctgag ccgccaactt cctcgaaaaa
     4021 tcccggtaaa gcgcaaatgc aaggccgact gatagtgtgt caacaatcgg ttgtaccgtc
     4081 ataaggccat ataagggaat tagtttatcc atgagaaaca tgagcggcac atcgattgtc
     4141 gttttgcgga ataccgagat ggttagtgct cgtttgcctt gacctgtggc ttggaacatt
     4201 gttgtaatga gatagcttat ggccatcatg ggcgtcataa gacatagaat tcgcagaaag
     4261 tttgcgccca gcctaatggt ctccggatca tttataaaga gcctaacaat aggctccgca
     4321 aaaatctcga agataataat gcagagaagc gagaagcata ccgcgacggt acgcgcgaac
     4381 ttgtttgcct ggtgaactct gtcgaatctc tttgctccat aattatatcc tataaaagga
     4441 agtacaccct gagagattcc catggtcaca ttcatcggca acatatcgat tttttttgca
     4501 attccgatcg cagccagggc agcggagcca tactttgccg taaggttatt taggactgtg
     4561 ttggaagtaa gcgatagaat attttgtaga gatgccggca atccgacaga gaccactttg
     4621 gctgcaatgc tgaattcctg attcagatcc cgtggtacaa aggagatgat ggttttgcct
     4681 ttcttgtatg caagatatac aacgaaataa caaacaaccg cgcagttcga taaaagagtt
     4741 gcgatagccg caccctttac ttccaggcca aatgtaaaga tgaatatcgg atccaggatt
     4801 atattgagga tacctccaaa ggacattccg atgctcgcct gttttgcccc tccttcggag
     4861 cgcaccaggt gtccaaaaag catacccatt accgtaggta tgcctccgat caccagaacc
     4921 catattatat agttgctcgc aaaatcatag gtctcctggt tagctccaag gagcatgagc
     4981 agcggtctct ggaatataaa tgccagtatc gaaaagatgg cggcaacgaa aattgcggaa
     5041 agcaagcaaa agctagatgt ctgtttggct ttggcgggtt ggcctgatcc aaggaatcgc
     5101 gatatgacac tactgccacc cactccaaag agattggcaa tagccgttaa cattgtatat
     5161 gcgggcatga cgagcgagac cgcagccacc tttcttggat cgcccaattg accaacgaag
     5221 aaagtatcag tgacattgta gatcattgtc acgagttgac ttattacggt tggcaaggca
     5281 agactagcta ctgcagatgg gatcgaagca ctacggaata cttctattct tgctgcatca
     5341 tgggctggag ctacatatac gctttcatgt tgaacatctt ccaaatcgca cgaaccacct
     5401 ttcttattcg tttttccatt cccaggaata acatagaacc ctagattatc ctaatacaca
     5461 aataatttaa aagggttatg aaatcccgga aaaggatctc atcgaagata acccagccat
     5521 tgtgcctgtc gcgaaatcat aacatccaca agttccggga cttgctcgca gataggcaca
     5581 accggattga ccaaaagagc ctgaattact aggtccttgc ttttctggag aatcgcctcg
     5641 gcggtgagat cgtagacacc ggtgtaattg cgcaagagag cggcaaaagc ccttggataa
     5701 tcgggaaata ccacaccttc tatacccttt ctgtgcacgc gagcaggtac ttccacggtt
     5761 acatttccgg gtaagcttgg gatgagtcca ttgttcatta tattgacagc cgcttcctca
     5821 taacccgagt cttggagtat tccttcgata attggcacca agcgctcctt taccttaagc
     5881 tcgattttgg gctggagcaa gctcaaggag ctactataca gcagatagaa atcgagtatt
     5941 cccttattgt ctgcgacact gtgagcccaa ggaatatatt ctccaatatg gctgtcagag
     6001 gtgataggca ggaggtggaa atgctctaat atttgtctga agagctttct atcggcccat
     6061 tgcctcgatg agagagtacg accaatatct ggcaaatcag tctcaccttc ggttttgggt
     6121 atatttcctg tctcctggat gtattccagg atatccgaat aaccgatcat tctttcgaaa
     6181 aaggccggcg ctttggctaa gatttcggga tatgcgtctt ctcctgtctc gcgatagcgg
     6241 gcagagataa ggacgctaaa atgattgagt ccgcctgccc taagctcgag tttttcgaga
     6301 gggacaccaa gcattggggg gatatagcga gcgagcgagg ctatctcatg gcataatccg
     6361 atgaccttca gccctgggaa ttttcgaaga actgtagtgg tgatggaagt cataggattg
     6421 gaaaagttga aaattgtcgc atcgggacag agctcagcac aatcttgaca aatttcgaga
     6481 ataggcggaa caattcgaag cgcatgaaaa agtccgcctg cgccgccatt ctcaccatag
     6541 acctgaggta tgccatattg taatggaatc ttccaatctt gatcccacag ttcgaatcga
     6601 ttacctactt ctatggagat aatgacaaaa tccgctccct taagagcctc tcttcggtcg
     6661 gtatgcgccg atagggtaaa attgagtcca ttagcctctg caaaagcttg tgcggcctta
     6721 tagacattgc caagggcttc tccatcgata tcgacaagcg caatatgaga acctttgagc
     6781 aaagtgctcg aaaaaatgtc gcccagcgtg ccaaatccaa actgtgcgct tccagcgcca
     6841 ataagtacga ttctcattgt tattcctcca ataatctcga tccagaatac tatcgatata
     6901 taaaggggag gcgtgcatcg ctttacttct caatgaatta ttcgcgaacc aaaaccgtcc
     6961 ccgatttaag tatccaaaag agagaatccc tttagatcca ctatctgcaa atagactatc
     7021 cccgaatcag agatacaact accactatag ccgcgagcgc gaatctatac catacgaaca
     7081 gctccgccga atgcttctta aggtaggaca aaagatattt gatacaaaaa aaaccactca
     7141 cggctgccac aatgaatcct atcgggaaaa tagataattc gcttttacca atggttcctg
     7201 cgcctatctg ttccgaaaaa tcgtagagac tttttaggcc tgctccggcg attataggcg
     7261 cagaaagcag aaaggaaaaa cgcgccgctg cttccctctc gagcccaagc gcaagtcccg
     7321 cagtaatagt cgaacccgag cgcgatacac ccggaatgac cgcaagagcc tgtgcaaggc
     7381 caatacagag agcatcgcgg gcctttatct ggttaaaact ccttctatgc tgtgcgtatt
     7441 tatctgcaat caagagaagg agtgcaagca gagcaataga tccagccata aatagcatcg
     7501 agctccgcgg tataggattg gaatgaaaag cttcgtttat cttggattcc atcaaaaggc
     7561 ccgagactcc cccggggata caagcgagca agatgtacca ggccatgagt cgatcaggat
     7621 cttcgcctat tttgaaatga atcacgctct tgaaccaagc cgagatgagc cgcgcccaat
     7681 cggaggcgaa aaagacaata actgccagta gcgttccgag atgaagcgct acatcaaagg
     7741 taaggcttgt gagagcagga ttgttccatc caaagagcca tggaatgatt ataaggtgcg
     7801 cggagcttga tataggaata aattcggcga ggccctggac cattccaaga attatagact
     7861 gaataatgag catgcatttt cctcttgggt aacgctatag cattctaatt tttactctgt
     7921 ttaaggttta tccgcaatat ggcatacagc cttgtcatcc ggggtgtctt tcactatcat
     7981 taaagctaat cttcctgagc ccatagatag gccaatcagg agataggaaa ctgctatgat
     8041 acttcagaac gcattcaagc cggctacgat aaagattggg ctggaaagcg aatataaaga
     8101 tgaacttcta gaagaattgg tagatatcct tgcaaaggac tatccgaaag atcggatctt
     8161 tccaagagaa gaagccttga gagctcttag aaaaagagaa gagaagatgt ctaccggtat
     8221 ttataaagga gtagcggttc ctcatgcgac attcgagagc atcgataagc ttcgtggcgt
     8281 gattggttta tctaaaaatg gtatagactt cgaatctctg gacggaaatc cggtgtacct
     8341 ggtattcatg ctcgtttctc ctcccagcca agcagaagcc catctcgagg ctcttcgaca
     8401 gatcgccctt cttattaaag atcatctctt gctcgaaaat ctcatgaaag ctactacacc
     8461 cgagaaggtg tttgccctga ttcgagaatt cgaagatagt ctcaacccat gaggttgtgt
     8521 tatggattca gcagatagca tcgatagatt gctcgaaata gagcagaaag cagctcagat
     8581 aatccaggaa gccgaagaga aggcttccgg attgttagtt gaagcaaaaa aacggtcgga
     8641 aatagtccaa aatcagaaaa tttctcaaat gcgcagggaa ctcgaggcag attttgcagc
     8701 ttatatcgat tctcttaccc aaaagagcca caaggagatc gaagagtatc gcttgagcct
     8761 taattccatt cccctaaacc ggaaatcgct tttggagaca ttggaggctc ttctccaaac
     8821 ggaaaactga catggccgaa acgctcgata tcgcctatct ctatgctcgc gtatgtgggg
     8881 ctttttcgaa catgagactg ggcgataagg ccggagaact catgcagaac tctggaggta
     8941 tcgtagggct atggagactc ctctttgatg aggaggcacc tagtatcgcc gaggcaaggc
     9001 ttgtcttcga ggccgaacgt agaataataa agcgcgccat agataacttt gttcgcctta
     9061 caaaaccctt ttcagattcc agcgaattca tccgtgcact gatttcgaaa tatgagataa
     9121 gcaccataaa atcgatgcta tttagacttc gttcaggcga accaaagcct gaggcgacta
     9181 cctatttttc aaatatcatc gggcaagccc tggctcgatg gcctcgcatt acggatatgt
     9241 tctcagatac tccatattca tggctcgaca gccattggct cgacaagata gcaatggccg
     9301 aaaacgagct cgacaagcaa tattatctgg aattgtggaa ggcggcgctc ggcttgccaa
     9361 ggcagaaagt cggagcgatt ccggagctga tacgatggga ggtcatctat cagaatatag
     9421 tgtgggcgat tcgtacgtcg cgctattatg gattgtcgaa agaggatatt gccgacacac
     9481 tcgtctattt gccgcaagag gacagtactt cgcttgctct ggagactttt tcttatgatc
     9541 ttgagagaat cggaagtttc gattcatggc gacttaagaa actactgtcc aaccagacag
     9601 ggatggttct agatgtcccc gctttggaga ttcgtgttct tgaagatcta ttttttatgg
     9661 ttaggcgaag cctgcatctc tatccattta gctatacgcc gatttactgc tatttcaagc
     9721 tactggacta tgagacatcg ctgctcttgg cggtactgga gagtaccagg ctaagtgtga
     9781 ccccagagga aaagataaaa tatgtgtgga ttccaggagg aggaacagca tgagcagcgt
     9841 gagcatgcgc aaggtcgagc tattgcttct caaatccgat atcgatgcgg tgctgaaata
     9901 tcttgcaacc aagcgttgct ttcagatcat ttatcccgat gagatcgagc ggcttgcaag
     9961 agaaaagaga gcgagctttg gactgggggg caaggaagag ttgttggcca gtgacaatgc
    10021 gtttaataaa gcgccttcga ctgcttcaac gatagccagg cctgcaatga acgtgaagcg
    10081 ctttgatgag tcgcatctcg aagaacgaca aatcaacgag cctgatctcg atgaggaaag
    10141 gctagatgat gcgcaaagga aacttgattt tataagccag ttccttggca tcgaaggccc
    10201 aaaagatatt atcgaagaca gccgtttgcc cgatgaagat atgctctcaa agctagaagt
    10261 gctcttcgac cgctgctcgc agctcaaagt gagcttagat gaggagcagg cgaaattgga
    10321 tgggcttgca gagtccatcc atgaagcaaa ggcttttgcc agcctttctc ggccatttga
    10381 agaattcgaa aaattttcct atgtctcgat tcagatcggc aaggtggcaa aagataaaat
    10441 cgaagagctt tctcagatgc ttggcgatag ggcagttata attcctctgg acgaagaagg
    10501 gactattctt gctgcttcta gccgcaaggg ccgctttgct ttcgagaccg cgctttccaa
    10561 agtgggattc gaaaaaaaga aaccaccgga agggctcaag ggtattccgt cggagacaat
    10621 gaacgcactc gaaggtgcct atcaggtagg aaagctccat tttgagaaac tcttgacgga
    10681 aaaaaaggca ctaaaggatc aatacagtga tttattgcaa attatagcct cctccattcg
    10741 gctcaagaaa gccatcgctt tggtggagag taagctcgaa aaaacgcgtt gggcctatcg
    10801 actttcggga tgggtgcctg aaaatatggt cgataaactt gtcaaagacc tcctcactat
    10861 gcttggaaac cggatctcca ttcgtgtcta cgatccgcct gagctggacc ttgccggcac
    10921 aaaggaaaag caagaaggag tgccggttct cttagagcac aattctttcg tatcggcatt
    10981 tcagggtatt gtcctatcct atggcacgcc tctatatgga gacatagatc ccaccccatt
    11041 tgttgctttc tttttcactt tgctgttcgc gataatgttc ggcgatttgg gtcaaggtat
    11101 ggtgatcgca agtctggggc ttgcaatgca caaggcaaag aaaggtcttc tggcgagtta
    11161 caagaaatat gcgcatgctt ttatcgctgc cggcgttggt tcgatgataa tgggactgct
    11221 ggtaggttct ttctttacga gcgataccgc tctcattccg atcgagagag ctttgacagg
    11281 ttttttcttg ggcaaacctg ccgatcgttt tttgcagata atgcctagag aaaatgtgag
    11341 cgccatgttt tatttctttg gattcacatt gggcgtgggc gttataatca actcaaccgg
    11401 gattgttatc aatataatca acctcatccg cagaaaagaa tatggtgagg ccttcttttc
    11461 gaagactggc cttgccggcg cgctattgtt ttggtgggca atagtaatgg tagttaggat
    11521 cattttgggg aacaagctgg gtatagtcga tgttgcaggc attggattac cactcctggc
    11581 tattatattc gccgagccaa taaagtcctt tatcgacaaa acttatggaa agccacatac
    11641 agaagaagct tcgaattggc taattggcgg aattatcgaa ctattggata tagtcagcta
    11701 ttttgcctcg aatacattta gctttttgag ggttggggcc tttgcacttg cccatgctgt
    11761 gctttcgttt gtgatattta ctatgggcag tcttgtgagc ggtaaatccg cttctgggct
    11821 ggcattagaa atcatagtat ttttatttgg caatgcagtg ataattgtgc tggaaggttt
    11881 aattgtgact attcaggtta tcaggcttca gtattatgag tttttctcga aattctttac
    11941 ccgcacaggt gagccattca aaccagttag ctttgcaggt aaagtataat gaagtcatga
    12001 tctataacat tatgttagaa ggagattttg tatgaagaag aaattgctat tcggtgttat
    12061 ggcctttatg gcttcagctg ctgtttttgc tcagacccag gctgaacctg cagccgtatc
    12121 gtcctcggta cagatggtaa agtatattgc cgctgctctc gcggttggca tagcggcgat
    12181 aggtggagcc atggctgtcg caaagatagg tgccgcagcg atgggagcca tggcagagaa
    12241 gccagacctt tcaggcaagg ctttgcctta tgtcggtctt gcggaaggca tttgtctgtg
    12301 gggattcttg gttgccgtgc ttattctgtt cctataaggg agaataggtt ggcatgatgc
    12361 ccctggcaag gctcacggat gccagagagc attattttag agggtactat ggcagagatt
    12421 tttgtgctag ctgaagaaga gcttctcata ggctttggca tgataggcat aaaaggagcg
    12481 gctatattcg atcgagatga tgcaattacg gcatttcgaa gcgtggtgcg cgacaaacgc
    12541 tattcgcacg aaggccagac ttttgacctc tccgactgca agatgctcat acttagcgaa
    12601 gatgtatcgg atatgatcgg cgaagaactt ttggaatggc aattgagcgg agactttcct
    12661 ttgattgttg agattccgcc gctcgcgggt tcctcggctc aacatactcg tctcgtcgat
    12721 gccgtgcgga aggcgattgg aatcaaaatc gaatagcaaa gcagaattag gaaaaagcat
    12781 ggaagagatc aaaggcacag aagccctgga acgagaaatt ctcgaagatg cgcgcaaacg
    12841 cgcagaacgc atcattcgaa aggccgagga aagcgccaga ctccttggtg tacaaaccga
    12901 gaaaaaaata gaagaagcca caactgctct tgtaggagag tatcaagcca aaaagaggat
    12961 cgcagaactc gaaatgcttt cgcgccttcc tctcgaaaag gcaaggctcg atatttcgta
    13021 tcgcgacgag atgcttcgca aggcgcttaa gggggcactg gaaagcatga atcctcgtct
    13081 ttttggcctg tggtgtgtga aaaggcttgc ctgccaagcc gagcttgttc gcaattcgcg
    13141 cgcaagagtg cttgtacatg gcctggattc agagacgatg agagacattg aagcattgtt
    13201 tgggcaaggt tctgacatct cgatcgagga agttccaacc atgaaggctc gcggcttggt
    13261 agtcgagccc atggatacaa gctatcgcat ttcgataacc gaaaaggagc ttttggaatg
    13321 gctcttagat gaaaagagag gcgagcttgc agcggcgctc tttggaagtt ctgcttgaat
    13381 acagaggttg gcgcaaagaa caagggaata taagcaaaga aatgagtgcg agaaaattag
    13441 ggcaagaaaa aatatgaata caagcattcg aacaggggta attaccagga tttcagggcc
    13501 tgttgtcctt gctcgaggac tggaagacgc agggctctat gatgtagtaa aggtcggaga
    13561 atccgggcta attggagaga tcataaagct ctatggcgat gaggctacga ttcagatcta
    13621 cgaagataac acgcttatgc gcgtgggcga gccggttgag tgcatgcata gaccactttc
    13681 agtggcgcta gggcctggcc tcataggttc gatctacgat gggattcaga gacctctgcc
    13741 ggtgctcaaa ggtctttcag gcgcttttct taagcccggg cttgctggcg agccgctcga
    13801 tgcaaagaaa aaatggcatt ttgtgccgct tctgcatgag ggcgattcaa taggtcaagg
    13861 tattgcctat ggaaggattc aagagacttc ttctattgag cataggctca tattcgatgc
    13921 gagagttcct gcgggaattg cggcttatgt ggctccagag ggcgattaca cagttgcaga
    13981 aacgcttgta aggtcggccg atggtgccag ttatacttcg gtttccaatt ggcctgtgcg
    14041 taaggcgcgt ccgtttaggg agaagcagat gacgcgcgag ccgcttgtaa cgggcttgcg
    14101 ggtcatagat gtgctctttc caattgcgaa gggcgggtgc gcggcggttc cgggcggttt
    14161 tggaacgggg aagacgatga cgcagcatgc aattgcgaag tggtgcgacg cggacattat
    14221 tgtgtacatt gggtgcggcg agcgcggcaa cgagatgacc gaagtcctga ccgagtttcc
    14281 tagccttgtc gatcctcgga ccggtcgatc gctcatggaa cgcaccgtgc tcattgccaa
    14341 tacatcgaat atgcccgttg cggcgcgtga agtttcgatt tatacaggaa ttacaatagc
    14401 agaatactat cgcgatatgg gatatcacgt tgcggtcatg gcggattcaa catctcgctg
    14461 ggccgaggcg ctgagagagc tgtcaggtcg gctcgaggaa atgcctgccg aagaaggttt
    14521 tcctgcctat cttccgacgc ggctagccga gttctatgaa cgtggcggca tggtcgccac
    14581 attagaacaa aaaattggct cggtgacgat catcggagcg gtttcccctc ctggaggtga
    14641 cttctctgag ccagttacac agcatacgag gcgatttatt cgttgctttt gggcgctcga
    14701 tcgtgacctt gcaaatgcgc ggcactatcc agcgatttct tggaacgatt cctacagcga
    14761 gtacaccgat gatttgatag ctcactggga tgcaatcgat ccatcctggg ataggcttag
    14821 aataaaggca atggatattc tcaaaaaaga gcgaaggctt tcggatatcg tgcgcctcat
    14881 tggcccagat gcgctgccag atgagcagcg actcatactc cttaccgcag acatgatcaa
    14941 aaacggattt ctacaacaga gcagtttcga caaggtcgat atgtattgtg ctcctcaaaa
    15001 gcaaactctt ttgcttcaat ctattcttgc tttccatgag cttgcggaga atgcaatcaa
    15061 gaatggtgtt cctcttccaa aggtatccgc gcttccgata cgggagaaaa tcgttcgcct
    15121 caaaagttcg ctagagaatg ataagttaga agaaggcaga gtcgtaatcc aggaaattca
    15181 agctgccttc gaacagcttg gcttaaatgt acaaggagcg gttctagcat gagaggtctt
    15241 gcataccatg gaatagagcg agccgatggt ccaattgtaa ttgccgaacg cagggagaac
    15301 attggctttt ccgagcttgt gcgggtcaaa gatcggcagg gagaatatcg gcttggacgg
    15361 gttgtggagt tatctgaaaa ggcggtagcc attcagcttt ttgcggagaa caccggcata
    15421 tctatagatg acgcctgggt cgaatatctt gaaaagcccc tcaccttccg tgtcggcgaa
    15481 ggcattgtcg gcaggatttt caatggcctt ggcgagccta tagatggtta tccacctata
    15541 atgtcctcgg acctcaggga tataaacggc cttcccatca atccgagcgc tcgcgtgtac
    15601 cccagggact tcattcagac tggaatttca gcgatagatg gcatgaatac cctcattcgc
    15661 ggccaaaaat tgccgatttt ttctggaaac ggtcttcctc acaataggct agccgcccag
    15721 atagtgaggc aggcaaaagt actcagcgga gaaagtcagt ttgctatagt atttgccggt
    15781 atgggaataa aatacgatgt tgctagattc tttatagatt ctttcgaaag ttccggtgtc
    15841 ctggctcgag tggtgatgtt cctatcgctg gcagacagtc catcgataga gcgccttgtc
    15901 acgccgcgca cggcgctgac cgccgctgaa tatctcgcat acgagaagaa catgcatgtt
    15961 ctcgtagtca tgaccgacat gacaaactat tgtgaggctc ttcgtgaagt ctcagcggcg
    16021 cgcaacgagg tgccttcaag aaaaggctat ccaggctatc tctattcaga cctagcttcg
    16081 atctacgaga gggcgggaag aatcgaaggc agcgagggat ccataactca gcttcctatt
    16141 ctttcgatgc ccaacgacga tatatcacac cccattccag atttgacggg ctatataacc
    16201 gaaggtcaga tcgtgctaga gcgagacctt tcgcagcgcg gagtgtatcc gcccattgca
    16261 gggcttccca gcctttcgcg cctaatgaag gatggcatag gggagggcat gacgcgagag
    16321 gatcaccgcg agcttgctgc ccagcttttc tctgcctatg cctatgtcca tactgtgcga
    16381 gaccttgcag ccataatagg agaagaggag ctttctgaca gggacaaaat attcctcaat
    16441 tttggtgatc attttgagag cgaattctta gcccaaggcg aattcgaaaa tcgcagcata
    16501 gagcagacgc tagatctggg ttggaagatg ctctcgcttc ttccagaatc ggagcttgtt
    16561 cgcattccgc ctcagattct agcaaaatat ctgccccata atgatagcga taatcggaat
    16621 gatcagaatg atcaggaaaa atcccacgaa gtggccattc taaaagggct aaaggagaac
    16681 taataaatgg ccgaaaaaat gcctgcgcct accaagacga atctcatcaa ggagaaacgc
    16741 aatctgcagc ttgcgcttca gggttacgat cttctcgaga aaaagaggga gattctcgtc
    16801 atcgagctga tgaagcgcat ggatagtctt gagctcctag agcgggaaat cgcagatatg
    16861 acagaaaaag cctatgcagc tttgcgaaag atgcttcttt tggtcggccg agagagagcg
    16921 cttgaaattt catctttgcc agtgcgcgaa atcactctta gcgcatctca tatcaatgta
    16981 agcgggatgc ggcttccttc gctcgatgta catgctagta atcctacctt gaattattct
    17041 tttatgaatt catttgccat atgtgacgaa accgtgatag agtttactga gcttcttgac
    17101 aagctatcga cagcagcagg tatgaggtcg gttgtatggc gccttgcgcg agaggtccgc
    17161 aagactcagc gcagagtgaa cgcattggac aagatggtga tacctcgatc gcaatcgatc
    17221 gtgaaatata tcgatgcttc tctagatgag cgggagcgcg aatcgctgtt tgcagtaaag
    17281 atgctcaaga ggcggcaaat aggacagtaa gaatcatgaa gaaaatgcta gggaccatat
    17341 tggtagcgat caatggttca gatgcttcca tctgtgcttt caaatatgct ttggcgctcg
    17401 caaaagcctt tggctcgaag attgttgcct gctatgtagt cgacacggcg accattcgtc
    17461 agcttgcact ttccagaatc ttcgtgcctg aggagagcga agaatacgag cgcaatctcg
    17521 agaattcggg tcagcgctat ctccattttt gtgcagagct tgctcatcag aaacagctag
    17581 atatagagac atccatccgc cgaggctcgg tttctggaga aatagtcaaa tattcgatgg
    17641 aaatcgatgc tgatataata gtgctaggtg gtaatccggt cgatgtgatc tatcgcgatg
    17701 cgattgccga ttccaatcac gaggtgctca aaaatgcccg atgtcctgtg ctctttgtta
    17761 agcccccgat aggggatgat ctgtataggt ctatataaca ttgaattggg tgggcttact
    17821 taagtcagtt ccatgacctt caatatcttt ttatttgctt tctttttttc atctagttgc
    17881 atgctcaaag
//
LOCUS       DAOMZL010000034        17326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_2930_length_17326_cov_11.981356, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000034 DAOMZL010000000
VERSION     DAOMZL010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 17326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17326
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2930_length_17326_cov_11.981356"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="PLQ30_04320"
     CDS             <1..965
                     /locus_tag="PLQ30_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPW01702.1"
                     /translation="LAYNLYTSEISQTPGMASTVSIILMLCASVVIVLQQWVSSRRKY
                     ASMLVNRPVIKQIKGIKSVLAHILCYGIVFFSTLPLAVIVYFSFRKTSGPVFKPGFSL
                     DSYRQIFFDVPKTVTNSFLYSITAVLLIAFVGTLLGFVIARKRNAAVRILDPLLMIPY
                     MVPGTVLGIGFIVAFNKPPIILTGTAAIIILTYFIRRLPYSVRSAASILKQIDPALEE
                     AGINLGSPPGRTFRTITLPLMQSGIISGAIMSWVTSMNELSASILLYVGKTMTMPIKI
                     YISVADGYFGTASAMSTILLFVTGIAMFIVNKYFNRGSETFIAA"
     gene            1014..1757
                     /locus_tag="PLQ30_04325"
     CDS             1014..1757
                     /locus_tag="PLQ30_04325"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPW01703.1"
                     /translation="MYTRFEPGYILDAHLIEELQGVKADGAFFATLDHDLDVFILRHG
                     QSKGNASNTYQGRYDFPLTAQGEKEARQAGEWLSQFKPDYILASPMLRAKRSAEIVSS
                     VIDLAHIEFDDILIEMDTGVFSGIDPSTAAQRYPDIWEDFYRRSWDAIPAAESSKSIY
                     ARSIIAWQHIRDIATRGASRIICITHGGLLQWLIRSTLGVHDWLPLLPITNCCISKYE
                     IEIIKKGVITFAQWTLINLHPLESSCKIR"
     gene            1829..2572
                     /locus_tag="PLQ30_04330"
     CDS             1829..2572
                     /locus_tag="PLQ30_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01704.1"
                     /translation="MRTKKAILIAACLLLGAMLQPFAYAVQGEAIIPGMRLLNLGEPL
                     GQDECAAVTGGDVRMSLNRDRTKLKVNVIDNEYEARLGRIPPKEVFEIDVHNRVVDTQ
                     KAPFMPADSTGRKFASSGAVTSKPSQFPEGYWSVTSVKPREDKYGPYMISTNAVGKVE
                     TYVNDGKGNYNASGVYKDTGYAVHSNTNSFDVSKSHGCLIIKQDDLSRVAGVLKKDRE
                     ENPKAVQKILVSPPATKKPRRLFGNRDDL"
     gene            2769..3683
                     /locus_tag="PLQ30_04335"
     CDS             2769..3683
                     /locus_tag="PLQ30_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01705.1"
                     /translation="MRRNFRVSSALFLILMMAMVLMVVSCKKAKVVEKPLEGGTDKTA
                     AQDIAAMPGPRPEAGANLPEIKKQNENILIVYKHTNPGYAEAYGFDTLILRALTDGSG
                     MIVGGTLYQRTASGEREFAQFNASISGDTISLAASSSEQKPRSYTIKTKADRVEVSGA
                     RDVVATLNKSLVFSSPDGSYKEIYSIDSSKENLLSEIQKANVTLEKGSWTYPENGKAV
                     YNQTKPEDTQNIEGFQITLWYQDKGDYRFATEGPEPLNEVYASGFAEALAGDYGLVNA
                     VMLDLMLSESTYLRPIYAFAISRKGLGK"
     gene            3903..4607
                     /gene="deoD"
                     /locus_tag="PLQ30_04340"
     CDS             3903..4607
                     /gene="deoD"
                     /locus_tag="PLQ30_04340"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPW01706.1"
                     /translation="MSIHIAAQKGQIADRILLPGDPLRAKFVAENYLEGAFCFNEVRN
                     MFGYTGTYRGKPISVMGTGMGMPSLSIYVNELIREYDVNKLIRIGTCGSMLEYLNVRD
                     IVLAMSACTDSAANHLRFKGMDYAPTASFRLLKAAWDAATSRGLRVFAGSILSSDMFY
                     TEDPEQWKLWAKFGVLAVEMETAELYTLAAKFGCEALSVLTVSDDLVKGEITSAEERQ
                     SSFNEMVELALDAIFA"
     gene            complement(4611..6011)
                     /locus_tag="PLQ30_04345"
     CDS             complement(4611..6011)
                     /locus_tag="PLQ30_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01707.1"
                     /translation="MLFSLGNLITLAIVIAMFVAYHLWTADNRSLEKLKRLGDKLKDE
                     LGSYVESKAEEIKHYGIDLDVQQKAAKVVLEKIQEAQATLDEKSDSITEIADCFKKYD
                     NVLSQLMDMTKRVDQNIAHLAEKNDFIQAITRRLEDADKEIDIIEKEMPKLKEQFAHD
                     AQQILDGFRDDILDQLHERLTGIINLFEKSRSDAESAVTEAASLKENIHEMVEAALSA
                     ATSRAQSIEDSAFATLRAHIANNLEELSHQTDARIEGLSQRIGELVNAINDKLASLAS
                     ETASQLAESDRRLSEARAALSAMTSDMARIQKESAEITVALSKEFKAERSSLEEQFEQ
                     FGQAFEIHRESFEREFLDEISALRKSLEDVKANSEELRSAAQGNIAEALDELERNVAR
                     ELESRRSALDERIDEWLNSMDAKIRKVSQDALEQRKAEEARLAQEVNAELKRLKDSLY
                     TQTQRIERDIEAFKSV"
     gene            6145..6351
                     /gene="rpsU"
                     /locus_tag="PLQ30_04350"
     CDS             6145..6351
                     /gene="rpsU"
                     /locus_tag="PLQ30_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002673965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPW01708.1"
                     /translation="MSQITVEDNEPLEKALKRFKRMVEKEGIIREWKRREYYEKPSAI
                     RNREKKALERKLMKKTRRSQEGRY"
     gene            6525..7010
                     /locus_tag="PLQ30_04355"
     CDS             6525..7010
                     /locus_tag="PLQ30_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPW01709.1"
                     /translation="MATEMPTRYLSKSLSIMPFMVIFILFAACNSGQGTDRPNPKLKT
                     VDLTIGNAKIKAEVALTEGERNRGLMYRSSLKDGEGMLFVFESDQKLAFWMKNTKLPL
                     SLAYILSDGTIVQIIDLVPFSEESRPSIRSVRYALEVPQGWFERSGIKVGDKVRIPSL
                     E"
     gene            7097..7585
                     /gene="def"
                     /locus_tag="PLQ30_04360"
     CDS             7097..7585
                     /gene="def"
                     /locus_tag="PLQ30_04360"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPW01710.1"
                     /translation="MFNILTYGESILSEKAHEVTEFDHSLEQLIQGMHEVMRRNNGIG
                     LAASQVGIPRRLFIVGIGDEPLRVFINPHIIARSQETTEFEEGCLSFPGLYFNVVRPL
                     AVDIEAVDMHGKPLRISADGLLARVIQHEYDHLDGILFIDRVSPVKRKRALAHYGRML
                     NM"
     gene            7604..8566
                     /gene="fmt"
                     /locus_tag="PLQ30_04365"
     CDS             7604..8566
                     /gene="fmt"
                     /locus_tag="PLQ30_04365"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPW01711.1"
                     /translation="MRVLFAGNPDIAVPSLESLYDSSHEVVGILTNPETKAGRGLRTN
                     STPVAQAALRLWGDSVPVLTFETLKSEARAAVLAILPDILVSFAYGRIFGPRFMELFP
                     KGGINVHPSLLPRWRGSSPIPHAIMFMDEETGISIQTIAPEMDTGELLNTHRIPLTGR
                     ETSASLSHLCAQLAGPMVVEVLDEIENGIAESRPQLGEPTYCRKITKKDGHIDWHRTS
                     REIDARIRAFDPWPGSFAFLRDMRLCILEAEPFDDFDPMSKAGYVTNPSPGTIIAVVM
                     QKGIIIRTGDGYIAVKRLQMATRKAVGFQEFACGARDIIGAVLH"
     gene            8656..9762
                     /locus_tag="PLQ30_04370"
     CDS             8656..9762
                     /locus_tag="PLQ30_04370"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPW01712.1"
                     /translation="MAKKDKKDIKDFTKILKETASKAPQKVSKVAKTVADSASEQHEH
                     ISKWTKEQKRTFVWFAIILFLVMVIVAVLVFFLALRGEERTMVPDVRRMDLADALVKL
                     QQRELYPRLTLRFTDNPLDRNLVLEQSPPPGSIVKAGRRINLVVSRGAVLDRVEDYSG
                     RNIDDLRLYLQGISTTTKTLVSIREPPLYVFDNSAPGTIIDQEPKPGKEISGPTVLDL
                     VVSKGPESRNIAMPSLLGLSMREMASKASTIPLVFSYKMRSAHEKETPGTVVEQSVQP
                     DTNLKQFDSVQITIATPEARKGFVSGVFEYSLPGYPYVVPVGLDVISPDGTRNSIYSV
                     KHPGGVFSAPFSVQTGSVLVLMVSGAEVTRKEVK"
     gene            9759..10895
                     /locus_tag="PLQ30_04375"
     CDS             9759..10895
                     /locus_tag="PLQ30_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01713.1"
                     /translation="MMNERGEIPVEIVHLYAYDVGRSIDLDKVASLVPAHRDIALAKR
                     RDTPAYLTLPKPLVLSISTEECNSKLFGKISAMAKIYEDGAITMIVRYCTMSTFEDLP
                     AKAHMPIRDVSGSSSIEQFAEASFRMVREALRSAIVGYKELSEGDRETYIAFCLLECP
                     GDDPQAFVNAHKDLAASLISGETVGTLHISQIEQILAKPFSYRKNDIAIFDLDRCLII
                     DPSADYDDVLMMAEHANYRLIELRELDFLLDEWLGEAERDIRRLYFSSRRKRSSERAL
                     RLKLAQIQGLRFDALFTLENLDNSSKIIGDYFLGSIYHRLCEIFNTDEWKFSVERRLN
                     ALQNIYELLKSDTTEQRMMTLEMVFIAVCIIFPILQILQVMLVK"
     gene            10892..13540
                     /locus_tag="PLQ30_04380"
     CDS             10892..13540
                     /locus_tag="PLQ30_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPW01714.1"
                     /translation="MRMKHPWNANWWFRQGQVEEEFLNEDWPGPRNANSAENRATTNT
                     ELSKTSDVQINVKGWKAIHLPHDMTEAPFNSFDERAFLRFGCYAKKLEKEDIEKIVQG
                     LGEERIAANRKPMLFLRFEGVSVSCRVWVNGRLASSHKGPYTPFEVRIDSFIPRSWQE
                     VCSKEAIEKEIDAKTARPIWILVEVDSSEDSAVPPFGGVVDYLAYGGIYRGVSLCASI
                     GARLSELWTVPRPRGDALDGPWLIHIRAEIETLQSGSIDNCCLIARLYQAGELINEGN
                     IFLLDSIINKGENRFSTDFSMSVDRPRLWDIDSPTLYDLELALHSEIGQELDYVHLRI
                     GFRTAEFGPSGFYLNQRRIFLRGLDRHQEYPYIGYAMGPDGQKCDAKILKQELGCIIV
                     RTSHYPQSPHFLDACDELGLLVFEELPGWQHIGDSEWQEQALRNLEDMILRDRNHSSI
                     VLWGVRINESKDNHEFYAKTNELARRLDPYRQTGGVRCHIRSELLEDVYTFNDFTYDG
                     KGTMYISEPSRVLPKYKKRAPYLITEHTGHMFPTKRFDQEERLVEHALRHAHVLDNAM
                     GNSRVAGCIGWCAFDYHTHKDFGSGDRICYHGAADAFRIPKYAGFFYGSQVSPSERLV
                     LEPASIFAKGERNASHLLPIYIFTNCDAVDVYRSESFIARFFPDKAHFANLPHPPIVI
                     DDLIGSLIETEAWPQRDFRLFRKLAGKAMALGENSFDIWDKLRMALFMRRQKLGIQDI
                     EELVLRYGMNWGASDEKIRLVGILDGKEVVERSFGADSAAKRLSIEPDALWLKSLDEE
                     EWPSTRIVVKALDQYDNIAPFLFEPYSIDIKGPARLIGPARRSLISGVSAFWISGKAK
                     KGKVSIAVACPRFEEQAVAELDIELE"
     gene            13665..14444
                     /locus_tag="PLQ30_04385"
     CDS             13665..14444
                     /locus_tag="PLQ30_04385"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPW01715.1"
                     /translation="MGKKAISENESTYTVTDARQILEALFLIEDFFRYGVRCEHEISL
                     EASASAKPSSENHKATLEALKAQVDQCTACGLWQGRSQIVFGEGVLNPSVMVIGEGPG
                     IEEDRQGLPFVGASGQLLDKMLVAIDLSRHSNCYIANIVKCRPPNNREPSPSECDACI
                     GYLRAQISILQPDYILCVGRIAAHSLLRLSDPISKLRGRDFDFQGIPVRVTFHPSALL
                     RDTGLKRPAWEDLKQFRTLMESGVTKQRSAGRATQFSRPEN"
     gene            14448..16562
                     /gene="priA"
                     /locus_tag="PLQ30_04390"
     CDS             14448..16562
                     /gene="priA"
                     /locus_tag="PLQ30_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPW01716.1"
                     /translation="MPMSFVRIAYPIPLQETFWYRNDTSTKAEIGMRVEAPLGRRAKA
                     IGYVVEVIDIPTSKSETVSRLESEDISPVKAVEPPLTKLGKPDLSADLEKPTLPPDLA
                     KLEPGKIRSINRVIDTEPLFDSEMLKLGLWMASMYHSSLGEALSAMLPSARRERSEIA
                     EQFDEIELSRTPLVLTQGQKAALDGILAEPKGMFYLYGPTGTGKTEVFLQLADAALSI
                     DLGVLYLVPEIALTRQVEQDARARFGERCAIIHSRLTPSRKLAEWRRIGKKEARIVIG
                     ARSAVFAPISDLGLIIIDEEHDASYKSGTTPRYHARQVAMRRARLEGARLVMGSATPS
                     PEAWQACREGQMLRFDLSNRPAGGSFPEMHIIDMRGRHGLISDELADALRDAKKAGRQ
                     SILFLNRRGFAHTLMCQTCGSQLLCKHCSVPLTYHKASNRLNCHYCGYHEIKPRACPS
                     CGSLDLAWASYGTERIEEELHERFPDMSHARLDTDTTNTKGYLEDTLLAFRNGKIDIL
                     VGTQMVAKGLNFPGVRVVGILMADQGLAMPDFRAGERVFSLIVQVAGRAGRHVPDGLV
                     FIQTRKPDSPIIQLASTGNVSGFLDRELQMRQQLEFPPATRLCRFVFRSKSAKSARSH
                     ASKCATLAHRLVCMSAFEGIDILGPSECPLAIVADNHRYQLIFRSDRFADLQRFVQTL
                     QHNIECTSSVYIEIDIDPVQMM"
     gene            16837..>17326
                     /locus_tag="PLQ30_04395"
     CDS             16837..>17326
                     /locus_tag="PLQ30_04395"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670456.1"
                     /note="involved in coenzyme B(12)-dependent 1,
                     2-propanediol degradation; important for the synthesis of
                     propionyl coenzyme A during growth on 1,2-propanediol;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HPW01717.1"
                     /translation="MVILTLNCGSSSVKYQVYDWEKKDVLANGIVERVTIGGSFITHK
                     VRNRPDYEVEHECPNHVVAIELILKTLVDPEVGVISDLSKIKAVGHRMVHGGSKFAKS
                     SIINGDFLETFESLNELAPLHNPANLMGVEAATTILPNVPQCAIMDTAWHQTMPASSY
                     MYA"
BASE COUNT         4680 a         3953 c         4219 g         4474 t
ORIGIN      
        1 cgcttgccta caatctctat acgagcgaaa tcagccaaac tccaggaatg gcttcaaccg
       61 tatcgattat tctgatgctc tgtgcgtcgg ttgtgatcgt gctgcagcag tgggtctctt
      121 cgaggcgcaa atatgctagc atgctggtca ataggcctgt gataaagcag attaaaggca
      181 taaaatcggt gctcgctcat atattgtgct atggtattgt ctttttctcg actcttcctc
      241 ttgctgtgat tgtatatttt agtttccgaa agacctcggg gccggtcttt aagccaggtt
      301 ttagcctgga cagctataga cagattttct tcgatgttcc taagaccgtg acaaatagct
      361 ttctatactc cataacagcc gtgctcctta ttgcttttgt tggaacactt ctgggatttg
      421 taattgctag aaaaagaaat gccgccgtta ggatactcga cccattgcta atgattccat
      481 atatggttcc gggcacggtg ctaggtatcg ggtttatagt tgcattcaac aagccgccaa
      541 taatcctgac gggcacggcg gccataatca tcctgactta tttcattcgg cgcttgccct
      601 actcggttcg ctctgcggcc tctattctca agcaaatcga tccggctctc gaggaagctg
      661 gtatcaacct gggctctccg ccggggcgaa catttaggac tatcactttg cccttgatgc
      721 aaagcggaat aatatctggt gcaataatga gttgggttac atctatgaat gagctgtctg
      781 cttctattct cctatatgta gggaaaacca tgacaatgcc cataaagatc tacatttcgg
      841 tggctgatgg ttattttggc acagcgagcg cgatgtcgac catccttctc tttgtcacgg
      901 gtattgcaat gttcatcgta aataagtact tcaatagagg aagcgaaaca ttcatagccg
      961 cttgagatct tctgagacct cattgattat gagccttaaa tccaggggat aacatgtata
     1021 cgcgattcga gccgggctat attctcgatg cgcatctcat cgaagaattg caaggtgtaa
     1081 aagccgatgg tgcttttttt gccactcttg atcatgatct agatgtcttt attcttcgcc
     1141 atggacagag taaaggaaat gcgagcaata cttatcaagg gcgatatgat tttccgctca
     1201 ccgcacaagg cgaaaaagaa gctaggcaag caggagaatg gttgagccaa ttcaaacctg
     1261 attatatctt agcaagtcca atgttgagag ctaaaagatc ggccgaaata gtatcttcag
     1321 ttatagatct agcccacatt gagttcgacg atattctcat cgagatggac actggtgtct
     1381 tctctggcat tgaccccagc acggctgctc agcgatatcc agatatatgg gaggatttct
     1441 ataggcgaag ttgggatgcc attcccgcag ccgaatcttc gaaatctatt tacgcacggt
     1501 caattatagc ttggcagcat attcgagata tcgccaccag gggagcttct cgcataatct
     1561 gcattaccca cggcggtctt ttgcaatggc ttataaggtc tacgctcgga gttcacgatt
     1621 ggctgcctct tcttccgatt accaactgtt gtatttctaa gtacgagata gaaatcatta
     1681 agaagggtgt cataactttt gctcagtgga ctttgataaa tctccatcct ttagagtcta
     1741 gctgcaaaat tagataattt ttcaaagaaa atcaaacttt gaagcgtatt tataccttct
     1801 tggcgtctat acaatgagga ggtgctaaat gcgcacaaag aaagcaatct taattgctgc
     1861 atgtctgctt ttgggagcga tgctgcagcc cttcgcctat gcagtacagg gtgaagcgat
     1921 catccctggt atgcggcttc tcaatcttgg cgaacccctt ggtcaggatg agtgtgccgc
     1981 agtaacggga ggggatgtgc gtatgtcgct gaatcgtgat cggacaaaac taaaagtgaa
     2041 tgtcatcgat aatgagtacg aggcaagatt ggggcgaatt cctccaaaag aggtgtttga
     2101 aatcgatgtt cataatcgag tcgtagacac acaaaaagca ccatttatgc cggcagattc
     2161 aaccgggcgc aaattcgcgt catctggtgc ggtaacaagt aagccttctc aatttccgga
     2221 aggctattgg tcagtaactt cagtaaaacc tcgggaagac aaatatggac catatatgat
     2281 ttccactaat gcagttggaa aagtcgaaac ttatgtcaat gatggcaaag gaaattataa
     2341 tgccagcggc gtctataaag ataccggata tgccgtacac tctaatacta attctttcga
     2401 tgtctctaaa tctcatggat gcctaataat caaacaggat gatctgtcca gagtagccgg
     2461 ggtcttaaag aaagatagag aagagaatcc aaaagccgtg cagaagatat tggtaagccc
     2521 acctgccacg aaaaagccgc ggagattgtt tggaaataga gatgatctgt agatcggaga
     2581 aaatcgatat aaaaatcgaa taagagatgt gtctttgccg ccatctatcc gcctaagggg
     2641 tatatgacag agaagatggc ggctttttga gttggatttt aataatatac agtgtaataa
     2701 attagattcg gctcttatct gagcagaatc aatgtagtat agtcatgagt attgagggga
     2761 ggtactgtat gagaaggaat tttcgtgttt caagcgcctt gtttttgatc ttaatgatgg
     2821 ctatggtact tatggttgta tcatgcaaaa aagctaaggt agtagagaag cctctagaag
     2881 gtggaaccga taaaactgct gcccaggaca ttgccgccat gccggggccg cgtcctgagg
     2941 ctggcgcgaa tctgccagag ataaagaagc agaatgagaa tatcctcata gtctataagc
     3001 acacaaaccc gggatatgcg gaagcctatg gcttcgatac attaattctt cgtgcactga
     3061 ctgacggatc aggaatgata gtgggaggga ctttatacca gcgtacagcg agcggagaaa
     3121 gagaatttgc acaattcaat gcgagtattt caggagacac tatatcgcta gcagcatcga
     3181 gctcggagca aaaaccacgg tcttatacaa taaaaaccaa ggcggatagg gtagaggtat
     3241 caggagcgcg cgatgttgtt gcaactctaa acaagtcgct tgtgttttct tcgcccgatg
     3301 gaagctataa ggaaatctat tccatcgatt cctccaaaga aaatcttctc tcagaaatac
     3361 aaaaagcaaa tgtaactctg gaaaaaggct catggactta tcccgagaac ggaaaggccg
     3421 tgtacaatca aacaaaaccc gaggataccc aaaacatcga aggattccag ataactttat
     3481 ggtatcaaga caaaggagat taccgctttg ccacggaagg tccagagccc ctaaatgagg
     3541 tgtatgcttc cggatttgcg gaggctctgg ctggcgatta tggacttgtc aatgcggtga
     3601 tgctcgattt gatgcttagc gagtcgacct atctgaggcc tatctatgct tttgccatat
     3661 cgcgcaaagg cttagggaag tagagcataa agcaagtctg aagcgtgggt cagaatacgc
     3721 tgaatataag gctgggaatg agataagaat taaggatatt aggctgcaaa tgagataaga
     3781 attaaggccg aaaatgctgc cgcggttgtt gccgtcctga aaagggattg agttctgttt
     3841 ggctttatga ttgaatacat aagccagtag gtaaaatctc ttggatagaa aaggactgcc
     3901 atatgagcat tcatatcgcg gcgcagaaag gccaaatcgc cgatagaatt cttttgcccg
     3961 gggaccccct cagagctaaa tttgtcgctg agaattatct cgaaggcgcc ttttgcttta
     4021 atgaagtgcg caatatgttt ggttatacag gtacttacag aggaaagcct atttcagtaa
     4081 tgggcacagg aatgggaatg cccagccttt ccatatatgt caacgaattg atccgtgaat
     4141 atgatgtaaa taagcttatt cgcattggaa cctgtggtag catgctcgaa tacctaaatg
     4201 tccgggacat tgtactggcc atgagtgctt gcaccgattc ggcagcgaac cacctgcgat
     4261 ttaagggcat ggattatgcg cctacggctt cattccgcct gcttaaggcc gcctgggatg
     4321 cggctacatc tcgcgggctg agagtctttg ctggttcgat attgagttcc gacatgttct
     4381 atacggagga tcccgagcaa tggaagctct gggcaaaatt tggtgttcta gccgtagaga
     4441 tggagaccgc tgagctctat acattggccg ctaagtttgg ctgtgaagct ctctctgtgc
     4501 taacggtctc cgacgatctc gtaaagggcg agataaccag cgcggaagaa cgtcaaagct
     4561 ccttcaatga gatggtagaa ctagcgctcg acgcaatatt tgcttgattc ttagacgctc
     4621 ttaaaagctt ctatatcgcg ctctattctt tgggtttgtg tatagagact gtctttgaga
     4681 cgcttaagtt cggcattgac ctcctgagcc aggcgggctt cctctgcctt gcgctgctcc
     4741 agagcatcct gagagacctt gcggatctta gcatccatcg aatttagcca ttcatctata
     4801 cgctcatcca aggccgatct tcgcgactcc aattccctgg ctacattgcg ctccaattca
     4861 tcgagagcct cggcaatatt gccctgggct gcgctcctga gctcttcgct attggccttt
     4921 acatcctcca aggatttgcg tagtgccgat atctcgtcaa ggaactctct ttcgaaggat
     4981 tcccgatgaa tttcgaaagc ttgaccaaac tgctcgaatt gttcttcgag cgaactgcgt
     5041 tcggctttga attctttaga aagtgctacg gtgatttccg cggattcctt ttgaatgcgc
     5101 gccatgtcgg aggtcattgc gctcaaggca gctcttgcct ctgaaagcct gcgatcggac
     5161 tcggcaagtt gagatgcggt ttctgaggcc aaagaggcta atttgtcgtt tatggcattc
     5221 acaagctcac caatgcgctg gctaagccct tctatacgag catcggtctg gtgtgagagt
     5281 tcctctaagt tgttggcaat gtgggcacga agggtggcaa aggccgaatc ctctatgctc
     5341 tgcgcacgag atgtcgctgc agagagggca gcctcaacca tctcatggat attttctttg
     5401 agcgaagcag cctcagtcac cgccgactct gcatcgctcc gagatttttc gaatagattg
     5461 attattcccg tcagccgttc atggagctga tcgagaatat cgtcacgaaa tccatctaga
     5521 atttgctgag catcatgagc gaactgctct ttgagtttag gcatttcttt ttcgatgata
     5581 tcgatttctt tatcagcatc ttcgagtcgt cgcgtgattg cctggatgaa atcattcttt
     5641 tcggcaagat gtgcaatatt ttgatcaaca cgctttgtca tatccatgag ctgggataaa
     5701 acattgtcgt attttttgaa acagtcggct atttcggtga tcgaatccga tttctcatcg
     5761 agagttgcct gggcttcctg gattttttcc agcacgacct tcgccgcttt ctgttgcaca
     5821 tcgagatcga ttccatagtg ctttatttcc tcggcctttg attcaacata ggagccaagt
     5881 tcgtccttga gcttgtctcc caggcgtttc agtttttcga gagaacgatt atctgcggtc
     5941 catagatggt aggccacaaa catcgcaata acaatagcta gcgttatcag atttccaagt
     6001 gaaaacagca tctaagaact cctcgctgaa agtatatcgt tcaaattaga tttacaaaag
     6061 agctgcaaca tataaactaa agcgcttgac aaaatatttg cttcagttac aatgcattcc
     6121 gcgtatagaa gggaggtgag aatcatttct cagatcactg ttgaagataa tgagccactc
     6181 gagaaggcat taaagcgctt caagagaatg gttgaaaaag aaggtatcat tcgcgaatgg
     6241 aagcggcgcg agtattacga aaagccatcc gctattcgca atagagaaaa gaaagcgctt
     6301 gaacggaagc tcatgaaaaa aactcgaaga tcacaagaag gcagatattg atcttcgagg
     6361 actagagtat gacacatgta gaaaagccgc ttctgcctaa gcggcttttt tatttttttt
     6421 agttgtttat tatggctgcg cttaaggcag cacaacttca aatttagagt ggtgtacacc
     6481 aactcaagtc tgaagtagcg catggataaa ggaaatgata gactatggca acagaaatgc
     6541 caactcgata cttatctaag agcttatcaa ttatgccatt tatggtaatt ttcattcttt
     6601 ttgcagcatg caactccggc caaggcacgg acagaccaaa tcctaagctc aaaacagtcg
     6661 atctcacaat cggaaacgca aaaataaagg ccgaagttgc gcttacggaa ggcgaaagaa
     6721 accgtggcct tatgtaccgc agctcactaa aggatggaga aggcatgctc tttgtattcg
     6781 aaagcgatca gaagcttgct ttctggatga aaaacaccaa gttgccacta tcgttggcct
     6841 atattctctc tgatggaaca attgtgcaaa ttatcgatct tgtgcctttt tcggaggagt
     6901 ctcgaccctc cattagaagc gtacgatacg ccctcgaagt acctcaaggc tggttcgagc
     6961 gctctggtat caaagtggga gataaagtac ggattccatc ccttgaataa aacgaaatcg
     7021 cggaacattg ccaagaagtc ttgttttgca ttaagttata cctgaaacgc aaatatgcct
     7081 aatataggaa attgatatgt tcaatatatt gacctatggt gaatcgattc tcagcgagaa
     7141 agcgcatgag gttaccgaat tcgatcatag tctggaacag ctcattcaag gaatgcatga
     7201 agtcatgcgg cgcaacaatg gaattggtct cgctgcttct caagtgggaa ttcccaggcg
     7261 gctctttatc gttggcatcg gtgacgaacc gcttcgcgtt tttatcaatc cccacatcat
     7321 agccagaagt caggaaacca cagaattcga agaaggttgt ctctcgtttc cgggcttata
     7381 tttcaacgtg gttcgtcctt tggccgtaga tatagaagcc gttgatatgc atggtaagcc
     7441 tctcagaatc agcgcagatg gcttattggc cagagttatt cagcatgaat atgaccatct
     7501 ggatggtatt ttatttatag accgcgtatc gcccgtcaaa agaaaacgag cgcttgcaca
     7561 ctacggccgc atgctgaata tgtaaaacga ctaggagtcg ccggtgcgcg ttttatttgc
     7621 aggaaatccc gatattgcag ttcccagctt agagagcttg tatgattcgt cacatgaggt
     7681 tgtcggaata ctgacgaacc cagagaccaa agcaggtcga ggtcttagga cgaacagtac
     7741 tcccgtagcg caagcggcat tacgcttatg gggagatagc gtgccggttc tcactttcga
     7801 gacactcaag tccgaagcgc gagccgcagt tttggcaatt ctacccgata tacttgtctc
     7861 ttttgcctac ggacgaattt ttggacccag atttatggag cttttcccaa aaggcggcat
     7921 aaatgtgcac ccaagccttt tgccaagatg gcggggcagt tcgcccatac ctcatgccat
     7981 aatgttcatg gatgaggaaa caggaatatc gatacagacg atagctcccg aaatggacac
     8041 cggagagctc ttgaatactc atcgaattcc actgactggc cgggagacaa gcgcttctct
     8101 ttctcatctc tgcgcacaac tcgcaggccc aatggtcgtc gaagtcctgg atgaaataga
     8161 aaatggaatt gcagagtcca ggcctcagtt aggggagccc acctattgca ggaaaataac
     8221 aaagaaagat ggccatatcg attggcatag aacatcgcgc gaaatcgatg ctcgcatacg
     8281 tgccttcgat ccatggccag gctcatttgc ttttcttaga gatatgcgcc tttgcattct
     8341 agaagcagag cctttcgatg attttgatcc tatgagcaag gctggatatg tgacaaatcc
     8401 ttctcccggg actataattg cggtagttat gcagaaggga attattataa ggaccggaga
     8461 tggctacatt gcggtaaaaa gactacagat ggcgactcgc aaggctgttg ggtttcagga
     8521 gtttgcttgc ggagcgagag acattattgg tgcggttctc cattagagag cttttagaga
     8581 gctttccgaa aaaggctcca tctcagcgat ggttataata tttgccgata attgcagatg
     8641 aaggaattct agagcatggc caagaaagac aagaaagata tcaaagattt tacaaaaata
     8701 ctgaaggaaa ctgcatcgaa agcccctcaa aaggtatcca aagttgcaaa gacagtcgcg
     8761 gatagcgcaa gcgagcagca tgaacatatc tcgaaatgga ccaaagagca gaaacgaacc
     8821 tttgtatggt tcgcaataat cctgttctta gtaatggtta ttgtggcagt attggtattt
     8881 tttctcgcgc ttcgaggaga agagagaacg atggttccgg atgtaagacg tatggatctt
     8941 gcggatgctc ttgtcaaact tcaacagcgc gaactatacc ctcgattgac tctacggttc
     9001 accgataatc ccctggaccg caatcttgtg ctggagcagt cacctccgcc tggaagtatt
     9061 gtaaaggcag gacgacgcat caatctcgtc gtgagccgcg gcgcagtgct cgacagagtc
     9121 gaagattatt caggtcgcaa cattgatgat ctcaggctct acctacaagg aatttccacc
     9181 acgacaaaga ccctcgtgag catccgagag ccgcctctct atgttttcga caattcagcg
     9241 ccgggcacaa tcatagatca ggaaccaaag ccgggcaaag aaatatcggg tccgactgtc
     9301 ttagaccttg tggtaagcaa ggggccagaa tccaggaata tagcaatgcc ttcgctcctt
     9361 ggcctctcca tgagggagat ggcgtctaag gcatccacaa ttccgctagt attcagctac
     9421 aaaatgcgtt cagcgcatga gaaagaaacg ccaggtaccg tcgtggaaca gtcggtccag
     9481 cccgatacaa atctcaaaca attcgattct gtgcagatca caatcgctac acctgaagcg
     9541 cggaagggct ttgtatcggg cgtattcgaa tattccctgc ccgggtatcc ctatgtcgta
     9601 cctgttgggc tcgatgtcat ttctccggac ggaacaagga attcaattta ttcggtgaag
     9661 catccgggtg gcgtattcag cgcgccattc tctgtacaga ctggctctgt gctggtattg
     9721 atggtcagtg gagcggaggt gacacgcaag gaggtgaaat gatgaacgag cgtggcgaga
     9781 taccagtcga gattgttcat ctttatgctt acgatgtagg gcgatcgata gacctcgata
     9841 aagtcgcttc tttagttcct gcgcatcgag acatagcctt agccaaacgg cgcgacacac
     9901 cagcttattt gactctgccc aaacctcttg tgctttccat cagcaccgag gaatgcaatt
     9961 cgaagctatt cggcaaaata tccgcaatgg caaagatcta tgaggatggg gctattacga
    10021 tgattgtgcg ctattgcacc atgtctactt tcgaagattt accggccaaa gcacatatgc
    10081 caattcggga tgtgagcggc tcctcttcca tagagcagtt tgccgaggct tcctttagaa
    10141 tggtaagaga ggccttgcgt agcgccatag tcggatataa ggaactaagc gaaggcgacc
    10201 gggaaactta tatagccttt tgtctgctgg aatgtcctgg agacgatccg caagcttttg
    10261 tcaatgctca taaggatttg gcagcaagct tgatatctgg agagacggta ggaacactgc
    10321 acataagcca gatagaacag atactagcca agccctttag ctatcgcaaa aacgacattg
    10381 cgattttcga tcttgatcgc tgtctcatca tagatccctc tgctgattac gacgatgtgc
    10441 tcatgatggc agagcatgcg aactacagac tcattgaact gcgagaactc gatttcctgc
    10501 tcgatgaatg gttgggagaa gccgaacgtg acatccgaag actgtatttt tccagcaggc
    10561 gaaaaaggtc tagcgaaaga gccctgagac tcaagcttgc tcagatacaa ggcttacgtt
    10621 tcgatgcact tttcacgctc gaaaatctcg ataattcctc caagataata ggagattatt
    10681 ttttgggctc gatctatcac aggctatgcg agattttcaa taccgacgaa tggaaatttt
    10741 cggtagaaag gcgtctcaat gctctgcaaa atatctatga actactcaag agcgatacca
    10801 cagagcagcg catgatgacc ctggaaatgg ttttcattgc ggtatgcatt atctttccaa
    10861 tactgcaaat ccttcaggta atgctggtta aatgagaatg aagcatccat ggaatgccaa
    10921 ttggtggttc agacaggggc aagttgaaga ggaattctta aatgaagatt ggccaggtcc
    10981 acggaatgcc aattctgccg aaaacagagc taccacaaac acagaattat ccaagacaag
    11041 cgatgtgcag ataaatgtta aaggctggaa agcgatacat ctgccccatg acatgaccga
    11101 agcccctttt aactctttcg acgaaagagc ctttttgcgc tttggatgct atgctaaaaa
    11161 gctcgaaaag gaagatattg agaagatcgt tcagggccta ggagaggaga gaatagccgc
    11221 caatagaaaa ccaatgctat ttctgcgctt cgaaggcgta agtgtatctt gtcgtgtgtg
    11281 ggtcaatggc agattagcta gtagccacaa agggccctat acgcctttcg aggtacgcat
    11341 cgattcattc ataccgcgca gctggcagga agtctgctcg aaagaggcta tagagaagga
    11401 gatagacgcc aagacagcaa ggccaatttg gattcttgtt gaagttgatt cctctgaaga
    11461 tagtgctgtg ccacccttcg gtggagtagt cgattatctt gcttatggtg gtatctatag
    11521 aggagtcagt ctatgtgcaa gcataggagc tagacttagt gagttgtgga cagttccacg
    11581 cccaagaggc gatgccctgg atggaccatg gctaatacac atacgcgcag aaatcgaaac
    11641 ccttcaatca gggtctattg ataattgttg cttaattgcc cgcctttacc aagctggaga
    11701 actcataaat gagggaaata tattcctcct ggattccata ataaataaag gggaaaacag
    11761 gttttcgaca gatttctcca tgagtgtaga tagacccaga ctctgggata tagacagccc
    11821 cactctctac gatctggagc tggctctaca tagcgagata ggccaagagc tagattatgt
    11881 gcatctgcgc attggttttc ggactgcaga atttgggcct tcgggattct acctgaacca
    11941 gcggaggata tttctgcgtg ggctcgatcg tcaccaggaa tatccatata ttggatacgc
    12001 tatgggtccg gatggccaaa agtgcgatgc caagatacta aaacaggaac tagggtgtat
    12061 aatcgtgcgt acaagtcatt atcctcaatc accccacttt ctggatgctt gtgatgagct
    12121 tggcctttta gtcttcgaag agcttccggg ttggcagcat ataggagata gcgaatggca
    12181 ggaacaagct ctaagaaacc ttgaagatat gatattgcgc gatcgaaatc attcgagtat
    12241 cgttctatgg ggagtacgca tcaacgaatc caaggacaat cacgagttct atgccaaaac
    12301 caacgagctc gctcgaaggc tggatccata tcgccagacg ggaggtgtta gatgtcacat
    12361 ccgcagcgaa cttctggaag atgtctatac cttcaacgac ttcacctacg atggtaaagg
    12421 aacgatgtat atctcggagc cctcgagagt gcttccgaaa tacaagaagc gggcacccta
    12481 tctaataacc gaacatacag gacacatgtt cccaacgaag cgtttcgatc aggaagagcg
    12541 actggtagaa catgcgctgc gccatgcgca tgttctagac aatgctatgg gcaattcacg
    12601 cgttgcggga tgcatcggct ggtgcgcttt tgactatcat acacataaag actttggttc
    12661 cggagatagg atctgttacc acggagcagc cgacgccttc cgcattccaa aatatgccgg
    12721 cttcttctat ggtagccagg tctcgccctc agagcgcctc gtattagagc cggcttcaat
    12781 atttgccaag ggggagagga atgcttctca ccttttgcct atctatatct ttacaaattg
    12841 cgatgccgta gatgtctata gatcagagag ttttattgct cgtttctttc ccgataaggc
    12901 tcactttgcg aaccttccgc atccacccat tgttatcgat gatctaatag gatcattgat
    12961 cgaaactgaa gcttggcccc aaagagattt ccgccttttc cgaaaactag ctggtaaggc
    13021 tatggcctta ggcgaaaata gcttcgatat ctgggataag ttgagaatgg cgctttttat
    13081 gcgacggcag aagctcggca ttcaagacat tgaagaactt gttcttcgtt atggcatgaa
    13141 ttggggggca tcggacgaga aaatacgcct tgtgggcatt ctcgatggca aggaagttgt
    13201 cgagcgtagc tttggcgcag acagcgctgc aaaaaggctc tctatagagc cagatgctct
    13261 atggctgaaa agccttgatg aagaggagtg gcccagcact cgcattgtgg taaaagccct
    13321 cgatcaatac gacaatattg cgccctttct atttgaacct tactctatcg atatcaaggg
    13381 gcctgctcgc cttattggcc cggcgcggag gtctctaata agtggagtca gcgccttctg
    13441 gatatctggt aaggcaaaaa agggaaaggt cagtatagcc gtcgcttgtc cgcgctttga
    13501 ggagcaggcg gtcgccgagc tcgatatcga attagaataa taagtcctgg tatcgaggac
    13561 cttgcgattc cagatgatga tagaagatga taggcgataa taggcgatga taagagatga
    13621 taggcgttca taacatttct attgaatgag ggttcgattt ggccatgggc aaaaaagcta
    13681 ttagtgaaaa tgaaagcact tacacagtta ccgacgctcg acagattttg gaagcccttt
    13741 ttctcataga ggattttttc cgctatggag taaggtgcga gcatgagata tctctggaag
    13801 cttccgcctc ggccaaacca tcgtcggaga atcacaaagc gactctcgaa gccctaaaag
    13861 cgcaggtcga tcaatgcacc gcttgcggcc tttggcaagg tcgatcgcag atcgtattcg
    13921 gcgagggtgt tctaaaccca agcgtaatgg ttataggtga aggacctgga atagaagaag
    13981 accggcaggg cttgcccttt gtaggggctt cgggccaact tctcgacaag atgcttgtgg
    14041 caatagatct ttcgcgacac agcaactgct atatagctaa catagtaaaa tgccgaccac
    14101 ctaataatcg cgaaccttcg ccttctgaat gcgatgcctg cataggctat ctgcgagccc
    14161 aaatatcgat attgcagcca gattatattc tctgtgttgg ccgcattgct gcgcacagtc
    14221 ttctcaggct ttccgatccg atttccaaat tgcgaggccg cgacttcgat ttccagggaa
    14281 ttccagtgcg cgtgactttt catccctcag ccctgcttcg agacacgggt ctaaagcgac
    14341 ccgcctggga ggacctgaaa caatttcgca cactcatgga aagcggagtc acaaaacaga
    14401 gaagtgcggg ccgtgcaact cagttttcca ggccggagaa ttagcgaatg cctatgtctt
    14461 ttgtacgtat agcctaccca attccgctcc aagagacttt ttggtaccgc aacgatacat
    14521 cgacgaaggc cgaaataggc atgcgcgtag aagctccact cggaagaaga gccaaggcca
    14581 tcggttatgt cgtggaagta atcgatatcc ctacttccaa gagtgagact gtctcacgtc
    14641 ttgagtctga ggatatatca ccagtaaagg ctgtcgagcc accactgacc aaattgggca
    14701 agcccgacct gtcggccgat ttagaaaagc ccacactgcc tcctgactta gccaagctcg
    14761 aaccaggcaa aatccgttct ataaataggg taatcgatac cgagccactc ttcgatagcg
    14821 aaatgctgaa gctaggtcta tggatggcct ctatgtatca ttcttccctg ggtgaagccc
    14881 tctctgcaat gctgccatcg gctcgtcgcg aacgatcaga gatagccgaa caattcgacg
    14941 aaatagaatt aagcagaact ccacttgtat taacccaagg gcaaaaggct gctctggatg
    15001 gcattcttgc ggagcccaag ggaatgttct acctctatgg accaacaggc accggcaaga
    15061 cagaagtctt tctccagctt gccgacgcag ctctctcgat tgatctaggt gtgctatatc
    15121 tcgtgcccga gatagctctt actcgccagg tcgaacagga cgcgcgcgcc cgattcggag
    15181 agcgatgcgc cataattcat tctcgtttga caccttcgcg caaactagcc gaatggcgca
    15241 ggataggaaa aaaagaagcg cgcattgtaa taggagcgcg cagcgccgtc tttgctccca
    15301 tctccgatct tggcttaatc ataatcgacg aagaacatga tgccagctat aaaagcggca
    15361 caactcctcg ataccacgca agacaggtcg caatgcgccg agcgcgcctt gaaggagcac
    15421 gccttgtaat gggcagcgca actccttctc cggaggcctg gcaagcttgc agagaaggcc
    15481 aaatgcttcg ctttgatctt tcgaatcgac cggctggagg atccttcccc gaaatgcaca
    15541 taatcgatat gcgaggtagg catggtctta tatccgatga gctagccgat gccttgcgcg
    15601 atgcgaaaaa agcaggccgc caatccattc tttttttgaa tcgtcgagga tttgcgcata
    15661 ccttgatgtg ccagacctgt ggttcgcaat tgctctgcaa gcattgttct gtgcctctaa
    15721 cctaccacaa ggcttcgaat cgtcttaact gccattattg cggctaccat gagataaaac
    15781 ctagagcatg tccatcttgc ggttcgctcg atctagcatg ggcgagctac ggaacagaac
    15841 gcatcgaaga agagttgcat gagcgatttc cggacatgag ccatgcgcgc ctcgataccg
    15901 ataccacgaa taccaaaggc tatctggaag atacgctgct cgcttttcgc aacggcaaaa
    15961 tagacatcct tgttggcacg cagatggtcg caaaaggcct gaatttccct ggtgtgcgtg
    16021 tagtaggcat cctcatggcc gaccaagggc ttgcaatgcc cgattttcgc gcgggagagc
    16081 gcgtattttc tctgattgtc caggtagccg gaagagcggg aaggcatgtc cccgatggac
    16141 ttgtctttat ccagacaagg aaacccgata gtccaattat ccagctggcc tcgacgggca
    16201 atgtttctgg ttttctcgat cgagagcttc aaatgcgtca acagctcgaa tttccacctg
    16261 caacgcgctt atgtcgtttt gtatttcgat ctaagtccgc taagagcgcc cgaagccacg
    16321 cctccaagtg tgccacctta gcccatcgcc ttgtatgcat gtcggcattt gaaggcatag
    16381 atattctcgg tccttccgag tgtcctcttg ccatagttgc ggacaatcat aggtaccagc
    16441 tcatattcag atcagatcgt tttgctgacc ttcagcgctt tgtacagaca ctgcaacaca
    16501 atatcgaatg cacttcttct gtctatatcg aaatcgatat cgatccagtg cagatgatgt
    16561 gataattcaa tttcaatagc tttgccatag acttgaggcc tgggttaggc taatcctgcc
    16621 gccttttttc ttgggtgctc gacaatgctg tggcaaaaca ggtggttact gtcgggtgta
    16681 cgatgatttt atgcaaaggt agccatgtgg cattttttcg agcccaataa tccccatctc
    16741 aataatcccc atcgcggtga acagggttta atttgttgca tagtctgccg gacggtgtta
    16801 tactagccgg aataatatct cctcaggagt tctaaaatgg ttatcttaac gctcaactgc
    16861 ggttcatctt cggttaagta tcaggtctat gattgggaga agaaggatgt tctcgcaaac
    16921 ggaatcgtcg agcgtgtcac aattggcggt tccttcataa cccataaggt ccggaacagg
    16981 ccagactatg aagtcgaaca cgaatgtccc aatcatgtgg tcgccattga gctcattctc
    17041 aagactctcg tcgacccaga agtcggcgtc atttccgatc tttcaaagat caaagcggtt
    17101 ggccatcgca tggtgcatgg tggctcgaaa tttgccaaat cctcaataat aaatggagat
    17161 ttcctcgaaa ccttcgaatc tctcaatgaa ctagcgcctc ttcataatcc ggctaacctc
    17221 atgggtgtag aagcggccac gaccatactc cccaatgttc cacaatgtgc aatcatggat
    17281 accgcctggc accaaaccat gccagcctcg agctatatgt atgctc
//
LOCUS       DAOMZL010000035        17260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_2950_length_17260_cov_14.046324, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000035 DAOMZL010000000
VERSION     DAOMZL010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 17260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17260
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2950_length_17260_cov_14.046324"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1451)
                     /locus_tag="PLQ30_04400"
     CDS             complement(<1..1451)
                     /locus_tag="PLQ30_04400"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HPW01718.1"
                     /translation="MANNSPSIALVLNAHLPFVRKPQYRQFYEERWLFEVISETYLPL
                     LRMFRKLELENIPFRVNMVLSPTLLAMLSDELLCERYIEYLDNQIELAQKERRRLAGD
                     PVFEPLADLYFAMYSRSREEFEDLYGRNIIRAFDYFSKKGYIELMTTAATHAFLPTYS
                     DIPEAIDAQIESAIIAHRMEFGKNPSGFWLPQLGWYPGLEKHLRAYNLKYTIVTTKGA
                     LLGSPSPFYGSFSPVMTASHIAAFIRDAGATKAIWSETEGYPAHSVYRDFYRDIGYDL
                     DPSYLAPHLYGVERGYTGFKYWAVTGKTDNKRPYEPSAASAQAIMHAHAYLSDRKVQA
                     RAASYWMKDRSPIIVCPYDAELFGHWWFEGIQFLEAFFRTMSRKDEENPLRLVTMSEY
                     LSENPSCPRSEPEFSSWAEGGYAEAWLDGRNDWVLRHTRRAAERMRELTIRFPNESGL
                     RERILNQAAREVLLAMSSDWALLLRSGKSADLAA"
     gene            complement(1451..2137)
                     /locus_tag="PLQ30_04405"
     CDS             complement(1451..2137)
                     /locus_tag="PLQ30_04405"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HPW01719.1"
                     /translation="MNPEILSLLSDEELRTLAEKMNIYVPEGLERIFLIEEIIDAYED
                     DTSDKVYSHDAPDHLEEKKLAGTGFIPGRLEDISIPDYYNETYINAIVRDPLWIFAFW
                     DIADSTRQKYIGEAETSRLLLRISEATGFEPSFSYDIVVSFDDRKWYINVPHPKRSYM
                     IDLCIAKCHKVRILARSNIVRMPSQYLDISSKLPQVTRSLLVLSGSEELHLIEPKEEN
                     SLRIMSMEGE"
     gene            complement(2184..3947)
                     /locus_tag="PLQ30_04410"
     CDS             complement(2184..3947)
                     /locus_tag="PLQ30_04410"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPW01720.1"
                     /translation="MSVVRTTPVPPDVDIAQSADIKPISDIASSYGLEEKYLEHYGTD
                     KAKVRLEFLSDRQGQSRRGKYIDVTAITPTPLGEGKTTTTIGLAQGLGYLGKKSIATI
                     RQPSMGPTFGIKGGAAGGGYSQIVPMTDFNLHLTGDTHAVSAAHNLCAAAIDARIYHE
                     SRWSDTYFEKLGLKKLNIDPYSVLWRRVVDMNDRTLRSIIVGLGGLENGPLRETGFDI
                     SVASEVMAVLALATSLDDLRRRIGKIVVALDKSGKAVTTEDIGVAGAMTILLKDALKP
                     NALQTLEEQLAFVHAGPFANIAHGNSSITADLIASEISDFVVTESGFGSDMGFEKLMD
                     IKCRSSGLMPDAVVLVATVRALKMHGGGPKVTPGKPLSSAYTQENLELLRAGLSNLIA
                     HIEIIGKYGLPVVVSINAFPSDTKAEHELIRDAALKAGAFDAVISRGWSLGGEGCAEL
                     ALAVDKASSQPHKANLLYPLDMPIKDKIEIIAHEIYGAGTVVYTPEADAAIGKYTDLG
                     YGNFPICMAKTQYSLSHDPAIKGVPRDFDFPVREVRLSAGAGFIVPITGEISTMPGLP
                     SKPAFIGMDIDTMTGRITGLS"
     gene            complement(4177..5157)
                     /locus_tag="PLQ30_04415"
     CDS             complement(4177..5157)
                     /locus_tag="PLQ30_04415"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HPW01721.1"
                     /translation="MRDSITDVPGIRVGNAQNQDKARGCTVVLCENARTAGVDVRGGS
                     PGTRETDLLNPVNMVQAPHAIYLSGGSAFGLAGADGVMQYLSENGIGFHLLNLVVPIV
                     SGAVIFDLTVGDPSAYPGPAMGREACMNASQEVSMGNVGAGTGALIGWAGNLNQRMKG
                     GLGTASASRGNLVVGAIVVVNCMGNVYDPETGELLGGTLDPTGSYILDAYEEMKNKQD
                     ALSLQADPEGLTTHTTIGVVATNAILSKPMATRMAMMAHDGYARSINPSHTSGDGDTI
                     FSVATGEVESNIDIVGSMAAEAMARAIAKAIRYADSAYGYKSYSELSKSL"
     gene            complement(5241..6122)
                     /locus_tag="PLQ30_04420"
     CDS             complement(5241..6122)
                     /locus_tag="PLQ30_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantothenate kinase"
                     /protein_id="HPW01722.1"
                     /translation="MNEDKIVTMCSPFFDLLKEETGMIAGIDIGSTSTKVVLLDNGCI
                     LCKMRVLASDAVAAAAGALGKLDTEKGYKIQSIDRIAITGAGASKIHQDIFDIPTVKI
                     EEIMAIGHGGKFLSKRDDIIIANIGTGTPFIEVNQNGIAHIGGTGVGGGTIIGLAKAL
                     IKTSDFNTIMDLASKGDLSRVDLMIKDIADTDISFLAKDMTASNFAKMLESATREDIA
                     MAIINMVYQVIGMLSVFVAQSRNKDSIIVTGNGNNNAIGRNVLGSISEMYGIHFEFPE
                     DAEFSTAIGAVLAASSA"
     gene            complement(6126..6950)
                     /locus_tag="PLQ30_04425"
     CDS             complement(6126..6950)
                     /locus_tag="PLQ30_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW01723.1"
                     /translation="MFGKSQKILFDLAVLYLLILMISAIAAPLIAPYNPTEQFLLKRF
                     HSPSLENIMGTDNFGRDILSRIIYGSRSALIVGIVTVAISLAIGMVIGLLAGMGTRSL
                     DNVLMLFMDSLLSFPTILLAITVVSFLGYGIVQVMLALGIISSPIFARLIRAETLAIK
                     TEGYIEAARALGTSSFKIAFKHIIPNLLGKVAVQCSLTFGQAIVIESSLSYLGVGTQP
                     PDASWGLMLKDARNYLIQAPWMAIYPGLCLALTVLAFNIIGDSLAESFNPRLTGSS"
     gene            complement(6957..8456)
                     /locus_tag="PLQ30_04430"
     CDS             complement(6957..8456)
                     /locus_tag="PLQ30_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW01724.1"
                     /translation="MKRSVILGMVFLALLSGVGSSLLAQGKGSIAFGLSGNPDTLDPQ
                     KTAGTLTFQVVKSFYDTLVEPDMTGKLVPALAESWTVSPDGLTWTFKLRPGVVFHNGQ
                     ALTSRDVKATLDRIRDPSFASPRRSEFTAIKEVKTPDPQTAVIILGAPYAPILANLAS
                     GWGAILPADLIQSGHNFDSEPVGTGPFKFEKWIRDNKISMVRNEKYWMKGLPKLARVD
                     FQIVPERSVQIQGLMSGAIDAVEYVDPEDLPILQSNPNITIRKEQTSMILVMAMNCSR
                     EPLNDLRVRQAINYAVDKQLVLDIAYGGGKVGSSFMDTGNAYYTDFSHFYPYNPDKAK
                     QLLKDAGVGNRQFTITVPQNYPLHVKAGELIQQMLEKVGMNVKIQLVDWSSWLARIYT
                     EGNFDLTIIGHTGKLDPDGTLAGYGTDKKYVRWINPTCDARIKEAATISDFAVRKKLY
                     TEALEIMAKEVPFVYLGSSYRYVAMRSNIADFRITPNLDSYDFRWTELK"
     gene            complement(8480..9466)
                     /locus_tag="PLQ30_04435"
     CDS             complement(8480..9466)
                     /locus_tag="PLQ30_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW01725.1"
                     /translation="MADLIESKDITKIYRSRGLAGSKHGFKAVDEVSVNIKKQSIFGL
                     VGESGCGKTTFGRALMYLDPPTSGSVIFDDVPLGSLKPKELRRFRNRMQIVFQDPNSA
                     LDPKMSIYNSLSEGMVNLKIPKEQRDRKVNDLINLVGIGKAYLRRFPHELSGGQKQRI
                     VIARALSMDPDFLVLDEPTSNLDVSIQAQIINLLIDLKNEFELTYLFISHDLNLVSYM
                     CDEIAVMFKGRIVEQAETELLISEPSHPYTQKLFSSIPGGAQRIGVKSLNIGEPGEKT
                     IREPQKEEKSQAGCSYYSQCPMGDAECSLSAPFLRELSRGHVVSCHKIRTGK"
     gene            complement(9451..10437)
                     /locus_tag="PLQ30_04440"
     CDS             complement(9451..10437)
                     /locus_tag="PLQ30_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW01726.1"
                     /translation="MERMASLLSIEDLRVAFSLGKKETTVLRGINLSILGNEIHGLVG
                     ESGSGKTVTSTCIIGLLPIPPARILSGHIYFDGKDLLSLPEDQMRTYRGRKIAMIFQE
                     PAKYLNPAFKIGEQIIEMLMLHMGMKKRSAYERVVELLRLVGLDEKVIDSYPHELSGG
                     MKQRAMIAMAISCDPDLIIADEPTTALDVTLQVQILALLKKLKDMRKLGVLFISHDLY
                     LVSEIANRVSVIYAGRIVESATKEELFRNPLHPYTRLLMHSIPDAKKKGSKLATIPGQ
                     VPDAAEIGNGCAFANRCPIAKEDCFNDIPSYVAYENAHTVACYYAGKIWQIL"
     gene            complement(10438..11364)
                     /locus_tag="PLQ30_04445"
     CDS             complement(10438..11364)
                     /locus_tag="PLQ30_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW01727.1"
                     /translation="MDLIRFIARRFLRLILSAFIIATIVFFVIRVIPGDPALIIAGID
                     ANPADVQAIRTRLGTDRPILVQYGDFIWHMVRFDFGNSMISGEPVLKLILERFPLTLK
                     LAIFGIAFSIVVALPLGVLSAVKRWSAWDYFGLVLSQLGMAIPSFWLGIILLLIFAIK
                     IPIFPLFGSETFAHYILPIVSLGFGRAAIILRHTRASMVEELSKEYIITARAKGLTER
                     MVKYKHALKNALLPIITVTSIELGYMLGGAIIIEQVFSMPGLGRLFLSGIYQRDFPII
                     QGGVVFVAILFSFINFIADILYSVANPKIRLS"
     gene            complement(11557..12774)
                     /gene="pcnB"
                     /locus_tag="PLQ30_04450"
     CDS             complement(11557..12774)
                     /gene="pcnB"
                     /locus_tag="PLQ30_04450"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HPW01728.1"
                     /translation="MRYFRNSNGSLKAKARIYGQEDHCIDISCIDREALAIVNRLVSH
                     GYSAYIVGGAVRDLLLGRRPKDFDIVTDALPNRIRKIFSNSRIIGRRFKLVHVYSNRK
                     IYEVSTFRSLSNGSVGNEYGTISEDVLRRDFTVNALYLDPIALEILDFVGGVDDIHSG
                     KLKAIIPLDRIFSEDPVRMVRGIKYAATNQFVIPFSLRLAIRRDASLLDSVSPSRLAE
                     EFYKIIATGKSVEIFQALDKYGLLQYLSPEVHASIHGDKAFKEAFLRDLASMDMLAQV
                     PEGTSLLNSATPAEIIQTDKRLISPFLAWFIRQRIKDFSFLRDISKDPVTFRHQIFSD
                     IRVFLSPLNLPRVALEEAMEQVLQEENLLPAPPPPKRRKQGQRKKKAKPRDESMHLKV
                     SDIDYTNAYKPGF"
     gene            complement(12820..13734)
                     /locus_tag="PLQ30_04455"
     CDS             complement(12820..13734)
                     /locus_tag="PLQ30_04455"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW01729.1"
                     /translation="MEKFRASDGKELAYLRFDPKNEAKAVVIVGHGMNDHKERFMHLA
                     EALTPIGVSVWIPDLRGHGDTDSGTNRGYLADKDGFERVVQDLIEMGNYASSCMGELP
                     LFYFGHSFGALIGMALIATYGKYLHGAVLSSPPEKQKLFMDKIGGLMISMGGALKGFH
                     APAKLPNQMTFGQYAKSVTDAKTKFDWLSRDSAIVDAYIRDPKCNFICTYGFYRDLLH
                     GVRKVYSNGFLESIPPSLPLFLLCGSADPVIGMKEGYEKLGQRFKLLGMIDFESKCYD
                     GGRHESINEINKQEVINDIIDWFSRHIQ"
     gene            13823..14455
                     /gene="tsaE"
                     /locus_tag="PLQ30_04460"
     CDS             13823..14455
                     /gene="tsaE"
                     /locus_tag="PLQ30_04460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPW01730.1"
                     /translation="MSSLDLQLYASFSSGLEKTDFREYYRIREYAEGGQYLIILSSPY
                     VRRTIAIGRAIGRAAPGGSIVAFRGDLGAGKTTLARGVAQGLGIAEPIISPTYTIIAE
                     YQGRLHLVHIDAYRLSDEDEFVQTGGEELLGSPGSLSLIEWSERIAGVLPNETQYITM
                     VVKEGGNRIILIEGDWIENIDWQRFKIPYSIDLQLASTLSKRYQNDCPVD"
     gene            14436..15098
                     /gene="tsaB"
                     /locus_tag="PLQ30_04465"
     CDS             14436..15098
                     /gene="tsaB"
                     /locus_tag="PLQ30_04465"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPW01731.1"
                     /translation="MTVLSIDTSIPTLRVGVSKEKTLAISALEPPVTHAEAILPAVEQ
                     VLQKAGITINNIELLAIAGGPGSFTGLRIGIATAKGLSLGLGIPMVLVPTLDIYGWAW
                     RELEGIVVPVIDARKHRVYCARYSFGQRIGDWMDIELTQLIWKIHGEDKVHFVGPDAE
                     LAEAICLEREGWILHRPNLEKEIESLSVLGINIYLEKGGANDTAGPIYLREPEIGTPI
                     AR"
     gene            complement(15099..16223)
                     /locus_tag="PLQ30_04470"
     CDS             complement(15099..16223)
                     /locus_tag="PLQ30_04470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW01732.1"
                     /translation="MPVEISKVESRKDLKRFIDFPVRLYWDQPNYVPAPRFDELRTLS
                     KDKNAAFEFCEAEYWTAWKDEKIVGRIAGIINHRFIEKWGKAWGRFGWIDFIDDSEVS
                     AMLFKTVEEWAASKGMEALHGPLGFTDLDREGMLVEGFNERATLATIYNYPYYPQHLE
                     MLGYRKDVDWLQFQIFTPKEIPEKVQHVADLLAKRSGVRLFEWKDKRVIVEKFGKQLF
                     ALIDETYGDLYGTTPLSERQVEDYIKQYLGFVDPRFTKILVDKDERLIGFGIAMPSLS
                     EAFYRSRGRLFPTGWYHVLKALNNPKVLDLYLVAVRPEYQSRGVLALVMNELQRSAME
                     AGVEYAETNLELEDNIKVQSIWKDYPKRQHKRTRAYKKEI"
     gene            complement(16207..16470)
                     /locus_tag="PLQ30_04475"
     CDS             complement(16207..16470)
                     /locus_tag="PLQ30_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01733.1"
                     /translation="MASWFDQKYMEVFDGTLNGLGHRRAVDPAFSIGDAENQLAHLYV
                     LDGNDWLGRGLLGDLISEATIAAYELFIEEWRTEKGGNDACRD"
     gene            16572..16644
                     /locus_tag="PLQ30_04480"
     tRNA            16572..16644
                     /locus_tag="PLQ30_04480"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16605..16607,aa:Met,seq:cat)
     gene            16792..>17260
                     /locus_tag="PLQ30_04485"
     CDS             16792..>17260
                     /locus_tag="PLQ30_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01734.1"
                     /translation="MNNLDRFRILSQAADWDAAAPSPLIEHTEIKFPDINSSDNAASE
                     IREDAAYNICKVKRLGGGSVPILKILLSNACSYNCAYCVNRRSSNIKRASFQPEELAR
                     TIADFNTRGIISGAFISSGVIGSPDNTMERLVWTAQTLRERYGYKGYLHLKVIP"
BASE COUNT         4738 a         4168 c         3597 g         4757 t
ORIGIN      
        1 gcggccaaat ctgctgattt gccacttctc aataagagag cccaatccga agacatggct
       61 agcagcactt cgcgagctgc ttgattgaga atgcgttctc tcagtccact ctcgttcgga
      121 aagcgaatag tcaactctct catgcgctca gcagctcttc tggtatgcct taggacccag
      181 tcattgcgtc catccagcca ggcctcggca tacccccctt ccgcccagga agagaattcg
      241 ggttctgatc gaggacagga gggattctct gacaagtatt cagacatggt tacaaggcga
      301 agtgggtttt cttcatcttt gcggctcatg gtgcgaaaga aagcctccag aaactggata
      361 ccctcaaacc accaatggcc aaagagctca gcatcgtagg ggcatactat gatgggagat
      421 ctgtccttca tccaatagga agcagcacgc gcctgcactt ttctgtcgga gaggtaggca
      481 tgcgcatgca ttatggcctg agccgatgct gccgatggct catatggacg cttattgtct
      541 gtcttgcctg ttactgccca atatttgaat ccagtatatc ctcgctcaac accataaagg
      601 tggggagcta gatagctagg atcgaggtcg taccctatat ccctataaaa atctctatag
      661 actgaatgag ctggatagcc ttcggtttca gaccagatcg ctttggttgc tccagcgtcg
      721 cgtataaagg cggcaatatg cgaagcagtc atgaccggag aaaatgatcc atagaatggt
      781 gagggcgaac caagcaatgc ccccttggtc gtcactattg tgtatttaag attataagcc
      841 ctgagatgct tctcaagccc cgggtaccat ccaagttgag gtaaccagaa tcccgaaggg
      901 ttctttccaa attccattct atgggcaata atggcagatt caatctgagc atcgatggct
      961 tcgggaatat ctgaataggt cggcagaaag gcatgagtcg cggcagtggt catgagctct
     1021 atatagcctt tcttggaaaa ataatcgaag gctcttatga tattgcggcc atagagatct
     1081 tcgaattctt cacggctcct gctatacatt gcaaaatata gatcagccag aggctcgaac
     1141 acaggatccc ccgcaagtct tcgcctctcc ttttgggcaa gctcgatttg attgtcgaga
     1201 tattcgatgt atcgctcgca aagaagctcg tctgaaagca tggctagcag agtaggagaa
     1261 agaaccatat tcactctaaa aggaatgttc tcgagctcca gtttgcgaaa catcctcagc
     1321 aaaggaagat atgtctctga aataacctca aaaagccatc tttcttcata gaactgacga
     1381 tattgcggct tgcgcacaaa aggaagatga gcattgagaa caagagctat cgaaggggaa
     1441 ttgttggcca ttattctcct tccatactca ttatccgcag agaattctcc tctttaggct
     1501 cgatcagatg aagttcctcg gaacccgaga gaacaagaag acttcttgtt acctgcggaa
     1561 gtttggaaga tatatcgagg tactgtgatg gcatgcgcac aatattggaa cgagctagta
     1621 ttctgacctt gtggcatttt gcgatgcata ggtctatcat gtaggaacgc tttgggtggg
     1681 gaacgtttat gtaccacttg cgatcatcga agctcaccac aatgtcatag gaaaaacttg
     1741 gctcgaaacc ggtggcttcg cttatccgca agagaagccg agacgtctca gcttctccga
     1801 tgtacttctg tcgtgtagaa tcagcaatat cccagaatgc gaaaatccac aatggatctc
     1861 ttacgattgc gttgatatat gtctcgttgt aataatccgg aatagaaata tcctctagcc
     1921 ttccgggaat aaaacctgta cctgcgagtt tcttttcctc cagatggtcc ggagcatcgt
     1981 gagaatatac cttatccgaa gtatcatctt cgtaagcatc gatgatttcc tcgatcagaa
     2041 aaatcctctc gagtccttct ggaacataga tattcatctt ctcagctaga gtcctgagtt
     2101 cttcgtccga aagaagtgac agtatttccg gattcacaat agtatagtga aagaatgatc
     2161 atgtccttgt caatatgcat gtatcaagac aacccagtga ttctccccgt catggtatca
     2221 atatccatgc caatgaacgc tggtttcgat ggcagtccgg gcatcgtact tatctcgccc
     2281 gtaatcggga caatgaaacc agctccagct gaaagcctaa cttcccgcac cgggaaatcg
     2341 aaatctcttg gaactccctt tatggcagga tcgtgggaaa gtgaatattg cgtctttgcc
     2401 atgcatatcg ggaaattgcc atagccaaga tcggtgtact ttccaattgc cgcatcggct
     2461 tccggcgtgt atacaactgt tccggctcca taaatctcat gagctattat ctctatctta
     2521 tcttttatag gcatatcgag aggataaagc aaattagcct tatgcggctg agatgaagcc
     2581 ttatccaccg ctaaagcgag ctccgcacag ccttctccac caagagacca gcccctgcta
     2641 attaccgcat caaaagcacc tgctttgaga gccgcatccc tgataagctc atgttcagct
     2701 tttgtatcgc ttgggaaagc attgatagag acaactacag gaaggccata tttcccgatg
     2761 atttcgatgt gagcgatgag atttgacaat cctgcgcgta gaagttccag gttttcttgt
     2821 gtataagcgc tggatagtgg cttgcctggt gtcacttttg gcccaccgcc atgcatcttt
     2881 agcgctctta ccgttgcaac aagaaccacc gcgtctggca tgagaccaga agatctgcat
     2941 tttatatcca tcaatttctc gaagcccatg tcagagccaa atccggattc cgttactaca
     3001 aaatcagaaa tctcgcttgc aataaggtct gcggtaatag aggaattgcc atgtgctata
     3061 ttggcaaaag gtcctgcatg aacaaaggca agttgctctt ctagcgtctg caaggcatta
     3121 ggctttaggg catctttcaa gagaattgtc attgctccag ctactccgat gtcttccgta
     3181 gtgacagctt tgcctgactt atctagagct acgacaatct ttcctatacg ccgtcgcaaa
     3241 tcatcaaggc ttgtcgcaag tgccaacacg gccatgactt cgctggcaac ggatatatcg
     3301 aatcccgttt cgcgtagagg cccattctct aagccaccta atccaacaat tatgctcctc
     3361 agtgttctgt cattcatgtc gacgacacga cgccagagta ccgaatatgg atcgatattg
     3421 agttttttca agcccaactt ttcaaaatac gtgtccgacc accggctctc atggtatatg
     3481 cgtgcgtcta tggcagcggc gcagagattg tgcgccgcag acacggcgtg tgtgtctcct
     3541 gttagatgca gattgaaatc tgtcattgga acaatctgac tatagccccc tccagccgca
     3601 ccgcccttta tgccaaatgt cggtcccatg gaaggttgcc ggatagtcgc aatggatttc
     3661 ttaccaagat atcccaagcc ttgagcaaga cctatggtgg ttgttgtctt tccttcacca
     3721 agaggcgtgg gcgtaatcgc ggttacatcg atatactttc ctcgcctaga ctgtccctgc
     3781 ctgtccgata agaattctag ccggactttc gccttgtcgg ttccataatg ctcgaggtac
     3841 ttttcttcca ggccatagct cgatgcaatg tcggatatcg gctttatatc cgcggactga
     3901 gcaatatcta catctggtgg aactggggtc gttcgaacaa cggacatgaa aaacctcctt
     3961 ttaataaaac ccggattcac aaaagaaata ggaaagacat gatcaactca acatgccata
     4021 tttggctaaa ggaaaaaaac atacagcatc aaggggatgg tcaagtcttt taaagtagaa
     4081 tttagcacag ataacgccga aagtgtagcg ggtccatctt ttggtaggta agatcgtcgc
     4141 cttcgaattt gcataatata tcgaaagctc caacaatcat aagctcttag aaagctcgga
     4201 atagctttta tacccataag cggagtctgc atatctaatg gcttttgcta ttgctcttgc
     4261 catggcttcc gctgccatag aacctacaat atctatattg ctttcgacct ctcctgtagc
     4321 tacagagaaa attgtgtctc catcgcctga agtatgtgag gggtttatag atcttgcata
     4381 tccatcatgc gccatcattg ccattcttgt ggccatcggc ttgctcaaaa tagcgttggt
     4441 agccaccact ccgattgtcg tatgcgttgt cagaccctct ggatctgctt gtaaagaaag
     4501 ggcatcttgc ttgttcttca tttcttcata agcatcaaga atatagctac cagttggatc
     4561 caatgtccct ccaagcaatt ctcctgtctc aggatcgtaa acattgccca tgcaattgac
     4621 aacaacaatc gctccaacaa ccagattgcc ccttgatgcg cttgctgttc caagcccacc
     4681 cttcattcgc tgattcaaat tccctgccca gcctatcaat gcccctgttc ctgcaccaac
     4741 attgcccata gacacttctt ggcttgcatt catgcatgct tctctgccca ttgccggacc
     4801 tggatatgcc gatggatcac ctactgtcag atcaaagatt accgctcccg atactattgg
     4861 aacaactaga ttcagcaaat gaaatccaat accattttcg gatagatatt gcataactcc
     4921 atcggcacct gccagcccaa aggcactccc cccactcaaa taaatggcat gaggggcttg
     4981 caccatattg actgggttca ataaatccgt ctcccgtgta cctggagaac ctcctcgaac
     5041 atccactccg gctgttcttg cgttttcgca taaaacaaca gtgcatcctc gtgctttgtc
     5101 ctgattctga gcatttccta ctctaatacc aggaacatcg gtgatcgaat ctctcatgat
     5161 aatagactcc tttgctttta tctcttaagc ggaacttaat ccgcattttt ctcggcatca
     5221 atatgaattg actataccag ctatgccgaa cttgctgcca ataccgctcc aatagcggta
     5281 gaaaactcag catcttcggg gaactcaaaa tgaatcccat acatttcgga aatactacca
     5341 agaacatttc ttccaattgc attattgttg ccattgccag tcacaataat actatcttta
     5401 tttctactct gcgctacaaa tacagagagc attccgatta cttggtacac catatttata
     5461 atggccattg cgatgtcttc tcttgttgcg ctttccaaca ttttggcaaa attcgaggct
     5521 gtcatatctt tagccaggaa actgatgtct gtatccgcga tatctttaat cattagatcc
     5581 acacgactca aatccccttt tgaagcaaga tccattatgg tattaaagtc gcttgtcttg
     5641 atcagtgctt ttgccaaacc aatgattgta cctcctccta ctccagttcc accaatatga
     5701 gcgattccat tctgatttac ttcaatgaag ggtgtcccgg tcccaatatt ggcgattatg
     5761 atatcatcgc gtttggaaag aaatttgcca ccatgtccaa tagccatgat ttcttctatt
     5821 tttactgttg gaatatcaaa tatatcttga tggatttttg atgcacctgc accggttatt
     5881 gcaattctat ctatgctttg aatcttatac cctttctcag tgtcaagctt gcccaatgca
     5941 ccagccgcag cagcaacagc atccgaagca agcacgcgca ttttacacag gatacatcca
     6001 ttatcaagca aaaccacttt tgtactcgta cttccaatat ctatgcccgc gatcatccct
     6061 gtttcctcct tcagcaaatc gaaaaatgga gaacacatcg tcacgatctt gtcttcattc
     6121 atattttaag aagaacctgt gagccttggg ttaaacgatt ccgcaagcga atcaccaatg
     6181 atattgaatg caagaactgt caatgccaaa catagacctg gatatatagc catccaggga
     6241 gcttggatta ggtaattacg tgcatccttt agcattagtc cccaactggc atctgggggc
     6301 tgagtgccaa cccccagata agacaaagaa gactcaatga ctattgcctg accaaatgta
     6361 agagagcatt gaacggctac cttccccaga agattcggga taatatgctt gaaagctatc
     6421 ttgaatgagg atgtccctaa ggctcttgct gcctcgatat atccttcggt ctttatagca
     6481 agcgtttctg ctcttattaa ccttgcaaat ataggactac tgattattcc cagtgcgagc
     6541 atcacctgca caattccata gcctaagaat gaaacaacag tgattgcaag caaaatcgtg
     6601 ggaaaggaaa gcagactatc catgaatagc atcaatacat tgtccagact tctcgtgccc
     6661 atacctgcta gcagccctat aaccataccg attgcaagcg aaatagcgac tgtcacaatg
     6721 cctacaatga gtgccgaccg agatccatag atgattctac ttaggatatc tctaccaaaa
     6781 ttatcggtcc ccataatatt ttcaaggctc ggcgaatgaa aacgcttcaa aagaaactgt
     6841 tcagttggat tgtatggcgc tatcaatgga gcggctattg ctgatatcat caatataaga
     6901 aggtacaata ccgctaggtc aaacaagatc ttctgggatt ttccgaacat actcttctac
     6961 ttcaattctg tccacctgaa atcataggag tccaggtttg gagtaatcct gaaatctgct
     7021 atattcgatc tcatagctac atagcgatat gagcttccaa ggtatacaaa aggtacctct
     7081 tttgccatta tctccaatgc ctctgtatat agcttcttgc gaacagcaaa atcagagata
     7141 gtggcagcct cttttattct tgcatcacat gttggattga tccatctgac atacttctta
     7201 tcggtaccat agcctgcaag tgttccatct ggatcgagtt tccctgtatg cccgatgatt
     7261 gtcaaatcga aattgccttc tgtatagatt ctggccagcc agctcgacca atctacaagc
     7321 tgtatcttga cattcatgcc aaccttttcg agcatctgtt gaataagttc acctgctttt
     7381 acatgtagtg gataattctg aggcaccgtg atcgtgaatt gtctgttacc aacgcccgcg
     7441 tcctttagca actgcttagc cttatcggga ttataaggat agaaatgaga aaaatccgta
     7501 tagtaagcat tgccggtatc catgaaagaa gagccaacct ttccaccgcc ataggcaata
     7561 tccaatacaa gctgtttatc tactgcgtaa tttatggctt gacgcacacg aagatcgttg
     7621 agtggctcac gagagcaatt catcgccata accaggatca tcgatgtctg ttcttttcga
     7681 attgtgatgt ttggattgga ttggagtata ggcagatctt ctggatcgac atattcaacg
     7741 gcatcgatag caccagacat caagccctgt atttgtacgg atctttctgg aactatctgg
     7801 aaatccactc ttgccaattt gggtaagccc ttcatccagt atttttcatt tctgaccata
     7861 gatatcttgt tatcgcgaat ccatttctcg aatttgaatg gcccagttcc aacaggttca
     7921 gaatcaaagt tatggccaga ctgaataaga tcagctggaa gaatcgcccc ccatcccgaa
     7981 gcaaggttag caagaatcgg tgcataaggc gcaccaagaa tgatcacagc ggtctgcgga
     8041 tcgggcgttt tcacttcttt tatggcagtg aattcgcttc ttcttggtga tgcgaagctt
     8101 ggatctcgta tgcgatccaa tgttgccttg acatctcgag aggtcaatgc ctggccatta
     8161 tggaacacaa cgcctggcct cagcttgaac gtccatgtca atccatcagg agagaccgtc
     8221 cagctctctg caagcgctgg cacaagcttg cctgtcatgt caggctcgac caacgtatca
     8281 tagaaactct tgacaacctg aaaggtcaat gttccagccg ttttctgagg atcaagcgta
     8341 tctggattac cggatagacc aaatgcaata gatcctttcc cctgtgccaa aagggaactc
     8401 cctaccccag ataaaagggc aaggaaaacc attcccaaaa taaccgatct tttcattttc
     8461 ttctcctgag aattgccagt catttgccgg ttctgatttt atgacaggac acaacgtgcc
     8521 ctcttgaaag ctcccttaaa aagggtgcag acaagctaca ttcggcatca cccatgggac
     8581 attgcgaata ataagaacaa cccgcttgag atttttcttc tttctgaggc tctcttattg
     8641 tcttttcgcc aggttctccg atattcagac tctttactcc tattctctgc gcccctcctg
     8701 gtatcgaact gaaaagcttt tgggtatatg ggtgacttgg ttcggatatg agcagttcgg
     8761 tttctgcttg ctccactatt ctgcctttga acattactgc tatttcatcg cacatatagg
     8821 atacgaggtt gagatcatgg cttatgaata gataggtcaa ttcaaattca ttcttcaaat
     8881 cgatcaagag atttattatc tgcgcctgaa tagatacatc aagattggat gtgggttcat
     8941 ccaatacaag aaaatcggga tccatagaaa gagcacgagc aatgacaatc ctttgttttt
     9001 gccctccaga tagttcatgg gggaatcgac gcagatatgc cttgccgatt ccgacaagat
     9061 tgataagatc attgaccttg cgatctcgct gttctttagg gattttgaga ttcaccattc
     9121 cctctgatag gctattatag atactcattt tgggatccag cgcgctattc ggatcctgga
     9181 atacaatttg catcctgttt cgaaatcttc tcaattcctt aggttttagc gacccaagcg
     9241 gcacatcatc gaaaatcact gaaccggatg ttgggggatc aagatacata agcgctcttc
     9301 caaacgtcgt cttgccacat cccgactccc caacaagccc aaaaatgctc tgctttttta
     9361 tattcactga aacttcatcg acagccttga agccatgttt tgatccagct aggccgcgtg
     9421 agcgatatat ctttgttata tccttgcttt ctataagatc tgccatatct tacctgcata
     9481 ataacatgca actgtatgtg cattttcata tgccacataa cttggaatat cgttgaagca
     9541 atcctctttc gcaattggac atcgatttgc aaacgcgcag ccattcccta tttctgccgc
     9601 atccggaacc tgtcctggaa ttgtggctag tttcgatccc ttcttcttag catctggaat
     9661 cgaatgcata agcaggcgag tataaggatg cagaggattt ctaaatagct cttcctttgt
     9721 ggcgctttcg acaattcttc ccgcatagat aacagagact cgattagcaa tttcgcttac
     9781 aaggtaaaga tcatgagaaa taaacaacac acccagtttt ctcatatctt tgagtttttt
     9841 cagcaaagcg agaatctgaa cttgcagtgt cacatcgagt gccgtcgttg gttcatcagc
     9901 tataataaga tcaggatcgc aggatatggc catggcaatc atggctcttt gcttcatccc
     9961 tcctgataat tcgtgtggat agctatcgat aaccttctca tccagaccaa ccaacctcaa
    10021 cagctcgaca actctctcat aagcagatcg cttcttcatt cccatatgaa gcataagcat
    10081 ttctattatc tgctctccaa tcttgaatgc aggattcaga tacttcgcag gttcctgaaa
    10141 tatcatagca attttcctgc ctcgataggt tcgcatctga tcctctggta atgacagcaa
    10201 atccttccca tcgaagtata tgtgtcccga taaaattctc gcaggaggaa taggcaggag
    10261 cccaattatg catgtggatg taactgtctt gccagatcct gactctccaa ctagtccatg
    10321 gatctcattt cctaaaatgg acagatttat gcctcgcaga acagtggtct ctttcttacc
    10381 aagagaaaat gcgactctca ggtcttctat cgataaaagg gaggccattc tttccattta
    10441 gctcagcctt atcttaggat tagcaacgct gtaaagtata tctgcaatga aatttataaa
    10501 tgaaaacaag atggccacaa aaacaacacc tccctgaatg attgggaaat cgcgctgata
    10561 gatgccagat aagaagagcc ttcccaatcc tggcatcgag aaaacctgct cgattatgat
    10621 tgcgccgcca agcatatagc ccagttcaat acttgtaacg gtgattatcg gcaagagagc
    10681 attctttagt gcatgtttgt attttaccat acgctcagta aggccttttg ccctcgcagt
    10741 gataatgtat tccttggata gctcctctac catggaggct ctagtatggc gcaaaattat
    10801 cgctgcgcga ccaaatccga gcgagactat tggcaaaata taatgtgcaa aggtctccga
    10861 accaaaaagc gggaaaattg gtatcttgat agcaaaaatc aaaagcaaaa ttatgcctaa
    10921 ccagaaactg ggaatcgcca tacccaattg ggaaagaacc aatccaaagt aatcccacgc
    10981 cgaccatctt tttactgcgg ataaaacacc aaggggcaat gcaacgacaa tactgaacgc
    11041 aatcccgaaa atcgcaagtt taagagtaag aggaaatcgc tctaaaatga gtttaagaac
    11101 aggttcccca gagatcatgg aattgccgaa atcaaagcgc accatgtgcc agataaaatc
    11161 tccgtattgc acaagaatag gacggtccgt acccaacctt gttcggattg cctgcacatc
    11221 ggcaggattt gcatcaatcc ctgcgataat caatgcagga tccccaggaa tgacgcgaat
    11281 aacaaagaat acgattgtag caattatgaa agctgaaagg ataagcctca agaatcttct
    11341 agcaataaag cgaatcaaat ccatgactgc ctactattac atcatctatt gatattagaa
    11401 tcgattgtat agataccgct tattattcat tgcagtactc ccttctattt gcgtaaatga
    11461 cgaaaatcca atctaattta aaactatact agccgtactg actgctgtca attaagacta
    11521 tttctttgct ttagagaact gtggtggcct ttattcctaa aaacctggtt tgtaggcatt
    11581 ggtataatcg atgtcagaga ctttgaggtg catcgactca tcccgcggtt tggctttttt
    11641 cttccgttgg ccttgttttc ttcttttagg aggaggagga gctggcaaga gattttcttc
    11701 ttgaagcact tgttccattg cctcctctaa tgccactcga ggcaagttga gtggcgatag
    11761 aaatacccga atatccgaga agatctgatg cctgaatgtg acggggtctt tcgatatatc
    11821 tctgagaaaa gagaaatcct tgatcctttg gcggataaac caggccagaa atggagatat
    11881 gagccttttg tccgtctgaa taatctcagc gggggttgcg ctgttcaata agcttgtacc
    11941 ctcaggcacc tgagctagca tatccatgga agccaggtct cttaagaatg cttccttaaa
    12001 tgccttatct ccatggattg aggcatgaac ctcaggcgat aggtactgca agaggccata
    12061 tttatccaat gcctggaaaa tctccaccga tttccctgtg gcaattatct tgtagaactc
    12121 ttccgcaagc ctcgagggac ttacggaatc aagaagtgac gcatcgcgtc gaatagccaa
    12181 tctcaatgaa aatggaatta caaattgatt ggttgcagcg tatttaatac cgcgcaccat
    12241 acgaacagga tcttcgctaa agatcctatc cagaggaata attgccttta acttgccgga
    12301 atggatatca tcgactccgc cgacaaaatc cagaatctcc agggcaatcg gatccaagta
    12361 aagcgcattc acggtgaaat ctcgccggag tacatcttcg ctgatggtcc catattcatt
    12421 gccgacagaa ccattcgaca gggaacgaaa cgtgctcacc tcgtatatct tcctattaga
    12481 atagacatgg acgagcttga agcgccttcc aatgatgcga gaattactaa atattttccg
    12541 gatacgattg ggcaaggcat cggttactat gtcgaaatcc ttgggccgcc ttccaagcaa
    12601 taaatcgcgt acggctcctc caacgatata cgctgaatat ccatgcgaaa ctagcctatt
    12661 cactatagct agcgcttctc tatcgatgca agatatgtcg atgcaatgat cttcttggcc
    12721 gtatattcgc gcttttgcct ttagactacc atttgaattg cgaaagtacc gcattctcac
    12781 aaagctgctc caaaatgcac gctcatacag tacaaaaatt cattgaatgt gtcgagagaa
    12841 ccagtcgatg atgtcgttga ttacttcctg cttattgatt tcattgatag actcatgcct
    12901 gcccccatca tagcacttgg attcgaaatc aatcatgcca agaagtttga atctctgtcc
    12961 gagtttttca tatccctctt tcattcctat taccggatcc gcagaaccac atagcaagaa
    13021 taaaggaagg gatggaggaa tgctttccaa aaaaccatta ctatagactt ttctaacacc
    13081 atggagaaga tcgcgataga atccgtaagt acagataaag ttgcatttag ggtctctaat
    13141 ataggcatct acgatagccg aatccctgga aagccagtcg aattttgtct tcgcatctgt
    13201 tacgctcttt gcatattggc cgaaggtcat ctgatttggg agctttgcag gagcatgaaa
    13261 gccctttaat gctcctccca tggaaatcat cagtcctcct atcttatcca tgaatagttt
    13321 ctgcttttcg ggtggagagg aaagcaccgc tccgtgaagg tattttccgt aggtcgcgat
    13381 cagcgccata ccaataaggg ctccaaagga atgcccaaaa taaaaaagtg gtaattctcc
    13441 catgcagcta gaagcgtagt tccccatttc aatcaaatct tggaccacac gttcgaatcc
    13501 atccttgtcg gctagataac ctctattggt gccagaatcg gtatctccat gacctcttag
    13561 gtctggaatc cagacagaaa caccaattgg cgtaagagcc tcggcaagat gcataaaacg
    13621 ctctttgtga tcattcatgc catgaccaac tataaccacg gcttttgctt catttttggg
    13681 gtcgaatcgc agataagcca gttctttgcc atcgctcgct ctgaattttt ccatctaagc
    13741 ccctcaatgt taaatgctaa tctttttata atgatcggta tatttcattc tcattcaatg
    13801 attattgtgc taagtgggct aaatgtccag ccttgatctt caattatacg cttctttttc
    13861 ttcaggtcta gaaaaaaccg attttcgcga atactataga ataagagaat atgccgaagg
    13921 aggacaatat ctgattattc tttcgagccc ttatgtgcgc agaacaattg caataggcag
    13981 agcaataggg cgtgcggcgc ctggtgggtc tatcgttgca ttccgcggag atcttggtgc
    14041 aggaaaaaca acgctcgcca gaggagttgc tcaaggatta ggaatagccg agcctataat
    14101 atcacccaca tacacgataa tagccgaata tcaaggccgc ctacatcttg tacatatcga
    14161 tgcatatcgt ctctcggatg aggatgaatt cgtgcagacc ggaggcgaag agcttcttgg
    14221 ttcgcccggc tcactgagtc tcatagaatg gagcgagaga atcgcgggag tattgccaaa
    14281 cgaaacccaa tacatcacca tggttgtcaa agaaggggga aacagaatta tcctaatcga
    14341 aggtgactgg atcgagaata tcgactggca gcgtttcaag attccctatt ctatcgattt
    14401 acagcttgct tcaaccttgt caaaaaggta ccaaaatgac tgtcctgtcg attgatacga
    14461 gcattccgac actgagggtc ggtgtttcaa aagaaaaaac gcttgccata tctgctctgg
    14521 agccgcctgt cactcatgct gaggccattc ttcccgcggt ggaacaagta cttcaaaaag
    14581 caggaattac gataaataat atcgaattac tcgctattgc aggcggcccc ggctcattta
    14641 caggtctgcg gataggaatc gcaacggcaa aaggactttc tctcggactt ggaattccta
    14701 tggtgcttgt ccctaccctg gatatatatg gatgggcctg gagagaactc gaaggcattg
    14761 tcgtgcctgt catcgatgca cgaaagcatc gagtctattg tgctcgctat tctttcggtc
    14821 aaagaatagg cgattggatg gatatcgagc tcacacaact aatatggaag atacatggcg
    14881 aagataaggt tcattttgta ggtccggatg cagagctggc cgaggctatc tgccttgaaa
    14941 gagaaggatg gatattgcat agaccaaacc ttgaaaagga aatagaatct ctctctgtcc
    15001 tgggtatcaa tatatatctg gaaaagggcg gtgccaatga taccgcagga cctatttatc
    15061 tccgtgaacc ggaaataggc actccaatag cgcgctgatt aaatctcctt tttataagcc
    15121 ctggtgcgct tatgttggcg cttgggataa tccttccata tgctctgaac tttgatatta
    15181 tcctcgagct ccagattggt ttctgcatat tcgacacctg cttccatggc actgcgctga
    15241 agctcgttca taaccagggc aagcacgccc cgagactgat attcgggacg aacagctacg
    15301 agatatagat caagcacctt tggattattc agcgctttta agacatgata ccaaccagtt
    15361 ggaaagagcc tgcctcgcga tctatagaaa gcttccgaaa gactaggcat ggctatgcca
    15421 aatcctatta ggcgctcatc tttatctacc agaatcttgg taaatcgtgg atccacaaag
    15481 ccaagatact gcttgatata gtcctctacc tggcgctcgc tcaaaggcgt tgttccatag
    15541 agatcgccat atgtttcatc tattagcgca aagagctgct tgccgaattt ttcgacgatg
    15601 acgcgcttgt ccttccattc gaacagccgc accccggagc gttttgcgag caaatcagcc
    15661 acatgctgga ctttctccgg aatctcctta ggggtgaaga tttggaattg aagccagtcc
    15721 acatcttttc ggtaaccaag catctccaaa tgctgaggat aatagggata attgtagatc
    15781 gtagctaagg tcgcacgctc attaaagcct tccaccagca tgccttcccg atcgaggtca
    15841 gtaaaaccca aaggcccatg cagcgcctcc atacccttcg aagctgccca ttcctcaaca
    15901 gtcttaaaga gcattgcgga gacctcggaa tcatcgataa aatcgatcca gccaaaacga
    15961 ccccatgcct ttccccattt ttctataaag cggtgattga ttatgcctgc aatgcggcca
    16021 acgatttttt cgtctttcca tgctgtccaa tattctgcct cacaaaattc gaaagccgca
    16081 ttcttatctt tgcttaatgt acgcagttca tcaaagcggg gagcgggtac atagttgggt
    16141 tgatcccaat aaaggcgcac aggaaaatct atgaatcgtt tcagatcttt ccggctttcg
    16201 accttactaa tctctacagg catcatttcc ccctttttca gttctccatt cttcgatgaa
    16261 aagctcatag gctgcgattg tggcctctga aattagatct ccaagcaaac cacggcctaa
    16321 ccaatcattt ccatctagca catataggtg agcaagttga ttttctgcat ctccaatcga
    16381 aaaggcaggg tcgacagcac gacgatgtcc taggccattc aatgttccat cgaacacttc
    16441 catgtatttt tgatcaaacc agctggccat tcaaacccca acttcaaaat cctgcaaatt
    16501 atagccttag aggtcaaaat cttacaaggt attgaccaag gggcattttt gagatataca
    16561 accacctgca tggcgggata gctcagttgg cagagcaagc ggctcatatc cgctgggtcg
    16621 tgggttcaat cccctctccc gctaggaatg cactaaacgc cctcgaaaga gggctttttt
    16681 attattttga tctactattc gaaaccggaa gaaactcttt tggaattgga atgcttatta
    16741 ttacctagga ttgacagata gcaatagtat tactaaactt gtgtttagta aatgaataat
    16801 cttgatagat ttcgtatcct ttcgcaagca gccgactggg atgcagccgc cccaagtccc
    16861 ttgatagagc acacagagat aaagttcccc gacattaata gctcggacaa tgctgcatca
    16921 gaaatccgtg aggatgctgc atacaatatc tgcaaggtca agcgtttagg agggggaagt
    16981 gtcccaatac tgaagatctt gctctcgaat gcctgctcct ataattgcgc ttactgtgtc
    17041 aatcgtcgta gctcgaatat caagcgggct tcgttccaac cagaggagct tgcccgcact
    17101 attgcggatt tcaatacaag aggcattatt tcaggtgctt tcatctcttc cggagtcatc
    17161 ggctctccag acaataccat ggaaagactc gtgtggaccg cgcagactct acgcgagcgc
    17221 tatggataca aaggatatct acacttaaaa gttataccgg
//
LOCUS       DAOMZL010000036        16910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_3038_length_16910_cov_14.417858, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000036 DAOMZL010000000
VERSION     DAOMZL010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 16910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16910
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3038_length_16910_cov_14.417858"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..1405
                     /locus_tag="PLQ30_04490"
     CDS             197..1405
                     /locus_tag="PLQ30_04490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW01735.1"
                     /translation="MIVEKRLLSLYICLFAVFALFGISMTVIGATLPKIFAEFGWSYM
                     TAGMVIAAGSVGYFLSSYLAGILFPFLGLRFLISLSLILICCGLEFFAKTPTAWLNTM
                     LYFIIGVGQGGIEIAVDWAVLRMEGPGGGKAMSLMHGAFSIGAVVGPLVIAILISANL
                     EWTLVYRIMGVLFLGIAIFMQFMPFKLLGKGRGQITNRSRRDLFHQPMYWLGFFALFV
                     YVGVELGISNWIAEYFVSVFGAPIPTGSFMVSLFWAGLLMGRFGIPIIQKSIKRERIL
                     IALSTLMAISLIAFNMIGFIGDGKLASVIAGILVILAGFGCSIIYPTIMSIIGDYFLH
                     AQSEAVGFAAMGGGIGGFLFPYMMSAVSTGWGIRIGFAIYALFSLLMVVLNIFLVRAQ
                     EQKSIGVSEP"
     gene            1436..1708
                     /gene="yccX"
                     /locus_tag="PLQ30_04495"
     CDS             1436..1708
                     /gene="yccX"
                     /locus_tag="PLQ30_04495"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPW01736.1"
                     /translation="MKAFHALIKGEVQGVGFRMSAVIRAERLGLKGWVRNTEDGDVEV
                     WAEGDPQALEQFYSWLQVGPAAARVDRVLKTDEIPKGNYIRFSVSF"
     gene            complement(1801..2409)
                     /locus_tag="PLQ30_04500"
     CDS             complement(1801..2409)
                     /locus_tag="PLQ30_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HPW01737.1"
                     /translation="MRQSFLSVTILLILITDPLGNIPLFIASLKHVPKGRRSEVILRE
                     CLIAFLVLVGFLFFGEVFLSALNLSDDILRISGGVILFLIALNMIFPGTGGKLVEDEI
                     EGEPFIVPVAIPLIAGPSAITYVMLLMKSDTSRALEWVGAIFIAILVTMVAFFMSNKL
                     KEWLGPRALSAIERLMGLVLTTIAIEMLLSGLLQYLSSVQNL"
     gene            complement(2547..3413)
                     /locus_tag="PLQ30_04505"
     CDS             complement(2547..3413)
                     /locus_tag="PLQ30_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPW01738.1"
                     /translation="MRSDIKGIMAMVATSVCWSLSGLFIKLIDWHPIAIAGARSLVAA
                     LFIFAVVRKPKLNFGKNQIIAAISYSCTMLLFVYANKNTTSANAILLQYGAPVYVMLL
                     SGVMLKEKPLPEQIGALIAIVIGMCLFFADSLSLGHIKGDVAAVLAGMTFAIHMLFMR
                     QQKEGSPIESLFISHCMTAIVSLLICIFLPPPVISKRSVAAILGLGIVQIGFSAVFFS
                     YAIKRITAIQSSLISIIEPALNPVWVYLAIGEKPSRMAILGGVIILSAVVISSIFSVS
                     RSARPSSMDGGT"
     gene            complement(3458..4261)
                     /gene="nagB"
                     /locus_tag="PLQ30_04510"
     CDS             complement(3458..4261)
                     /gene="nagB"
                     /locus_tag="PLQ30_04510"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HPW01739.1"
                     /translation="MRVVIQQSYELMSKWAAYYLVQHINEHDSQKPFVLGLPTGSTPL
                     GMYRELITLCKAKKVSFKNVITFNMDEYVGLTSEHEQSYHRFMWDNFFSHIDIEPKNV
                     HILNGMAIDLQKECTAYEKAICDAGGIDLFVGGLGADGHIAFNEPGSSLSSRTRIKTL
                     TRDTKKANARFFGGDPEKVPSQALTVGVGTIMDAQEVMILVSGMEKARALKHGIEMGV
                     NHMWTISCIQLHPKAIVVCDEDATNELRVVTVRYFKEIEAPNLRNWDLD"
     gene            complement(4258..5454)
                     /gene="nagA"
                     /locus_tag="PLQ30_04515"
     CDS             complement(4258..5454)
                     /gene="nagA"
                     /locus_tag="PLQ30_04515"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HPW01740.1"
                     /translation="MQSVFCIHNATVIAGYARMDRSAVLVEGDSIADVFSERRFAQKS
                     FGPQVHLIDAGGAYLTPGFIDTHIHGIGGFGTEDMSAEAILAMSEILPAWGVSSFVPT
                     LYPMPEEDMIRAIRAVVLAMGHETGAEIIGIHIEGPFISPAQLGVQRIEFVRPVDLEL
                     MQRLWDASEGHIVSMTVAPELKGMRELALFCNQLGIVLQAGHTDASYENMIEGMQAGI
                     RHSTHMFNAMSRLHHRNPNAVGAVLIQSDLSCEIIADGLHVHPDLIRLLFRDKPEGNI
                     VLVTDSLKPTKQAVNAPMFANDEEVYLEDNLFYRARDGVIAGSSLTMIEGVLNLVRFG
                     VPLESAVKMASANPARIFGLRRRGMISPGYKADLVLANADFDVQMTIIDGKIKYDHNA
                     IERRII"
     gene            complement(5478..6830)
                     /locus_tag="PLQ30_04520"
     CDS             complement(5478..6830)
                     /locus_tag="PLQ30_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01741.1"
                     /translation="MKRFVILLFAWLAVIASIGAQQAPAVQSPYSDAAPQTKEIIDAA
                     RLLEAKGQYSKAWNLLAAFDPDSKDGYVLAEKIRIALEDSIGNGMLVSFSFVDLAEGQ
                     SIYEAFLNPPEKQTIVDFNPLDLVEALEKSSEAIPPILSLELAGYLYAVNQQYSDNWM
                     IDGYTIQQKAVENYDRALAYGLYNDKSLANHAELLMNLQQYEGAEKVLRQAISLFPEE
                     LSFAVSLANSLNYQGKFDQVYSVIDKVIEDPDVTSSTYNAYVEGIKAGLNSGDTQKTD
                     LYVDAMIERFPDEYAPMLIQHLVAVIMGEIEEANVSADSVTEKFNVDPNVVQSLLSTW
                     LGASDPVSGFDYLNRSLEKYTSDDQAMGTLFFYRALMYAQTARSADDLKLAIVDMEET
                     EKRFSAVYDKEDPVFQTIEQLLTEWKQIIEQLQESDSASSSEQSTPPEDSNTASGTSN
                     "
     gene            7028..7675
                     /locus_tag="PLQ30_04525"
     CDS             7028..7675
                     /locus_tag="PLQ30_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPW01742.1"
                     /translation="MSRRYVVVAGNIGAGKSTLVSLLAEHLGFIAYLEPVLENPFLKD
                     FYANMSRWAFHSQLFFLSHRADMHRKLSEDPHSVVQDRSIYEDAEIFARNLYIQGIMA
                     EREWCIYKALYATLAEILPHPSLVIYIRASVSTLQSRIKQRGRDFESSIPDSYLEGLN
                     NLYEEWISSFTLAPILTIPGDSLDFVTESRDLQVILRTITKRLEDNQPYLFPYEM"
     gene            complement(8031..8192)
                     /locus_tag="PLQ30_04530"
     CDS             complement(8031..8192)
                     /locus_tag="PLQ30_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01743.1"
                     /translation="MIWQRNHVLSIREYGYIGPYDAVGNVVDLDLPADIAPYRAGGAG
                     YFGFQKNTS"
     gene            8326..9066
                     /locus_tag="PLQ30_04535"
     CDS             8326..9066
                     /locus_tag="PLQ30_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW01744.1"
                     /translation="MANMLFSIVLAFSLSAMTGYAQGWPEGKESDFFDTLKGKLEIVF
                     LGHGSLLLAYNGSNIYIDPVSQYANFSKYPKADLILITHEHNDHLDPKAIAALSKPET
                     RIILSEASRKKLGHGEVLQHGHSIDVAGVTVLAVPAYNITSSRMGYHPRERKDNGYVL
                     SIGSLRIYIAGDTEPIPEMANIGKIDIAFLPMNLPFTMTPTQVAQAARIIKPRILYPY
                     HFGSTDTSEITRLLSSDKDIEVRIRNLK"
     gene            9148..9675
                     /gene="tpx"
                     /locus_tag="PLQ30_04540"
     CDS             9148..9675
                     /gene="tpx"
                     /locus_tag="PLQ30_04540"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HPW01745.1"
                     /translation="MANITFKGNPIQTIGSLPAKGATASDFRLTKSDLSDVSLEDFEG
                     KIKILNIVPSLDTGVCAASARAFNKAASSIDDVVILTISRDLPFAQKRFCEAEGIDKV
                     ITLSELRTRNFGKAYGVEIISGPMAGLLSRAVVVLDKANKVIYTEQVPEIAQEPNYKA
                     ALEAARTATNLVYKH"
     gene            complement(9920..10813)
                     /locus_tag="PLQ30_04545"
     CDS             complement(9920..10813)
                     /locus_tag="PLQ30_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPW01746.1"
                     /translation="MIKPLEIHYEVRGYDCGYGGPLKPFALANFFQETAGVHATVLGI
                     GMEDMWARGLTWMLSRIDIRIEAMPRTGQRVIARTWPVGAKKIYAQRCLELLDESGFR
                     YAGALYEYIIVDMKTRRVVRPERTLPIDLTTDYPLPFVDLSPGVEDASFGAFPQATNH
                     SMQDKKDPYAEPKNTLLLNGFSEVFALEARTRHIDQNGHVNNAHFINWLCDAVPCADG
                     ETICRIKVDFVREILKGGKISVWIKPIHTISNSVSENALKQAEKIKAPHSSWLSALAC
                     DGILAAQAMITATNLVYNKLK"
     gene            11042..13459
                     /locus_tag="PLQ30_04550"
     CDS             11042..13459
                     /locus_tag="PLQ30_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPW01747.1"
                     /translation="MCGIVSLVYRDENPSIGKEAEALLKRLEYRGYDSTGAAFIGADK
                     RILLRKKVGAPSRVCPLLQIDSFSGQRFIGQVRWATYGSVTDVNSQPHHVHCKMELVG
                     AHNGNISNTDTLKTWLSARGHAVISDNDGEIIVHLIEEHYSAAQSLASSDLAYLRKAY
                     ANAGLAEGVPDGVLRMIEAIRRAQALAQGSYAAAIADPQLPGVFAIKSGSSLYAGVGS
                     DSFGNFIVLSSDLTSVLSKTRTLIPLSEGEGIWFTDKEYLVFNLQGNPFFSHPRLKRS
                     KLSIKDTQLSPEYQHYMEQEISSSMNNINQILRYYFRDSSTEPLSELFEDHKDDCKEA
                     AEKISSLSERFGNEALVSGMKEIFESPAWNEIEARVQNTGAAHFVGQDFISDETELLK
                     ELAALNPPMRGKLLLMDKVIVWRKRRAVLRYTGELKNAIRETTQSGGRIYFIASGTSY
                     HAALIAAYFFNSTAHIPVYPCNPGMFRSMYFNCMGKNDLIIGISQSGETKDLVDIFQD
                     VKKTNNSVRHVSIVNNENSRIPQELSDFYLPLLCGPEIAVAATKSFTSQIAILYLLAA
                     SFSMPEHKVRENLIKARDLMTETIETRMGDIESIAAHLFMASSIHILGTGLIGLAKEG
                     ALKIREIVLNHTEGYDAAEFKHGPNTILGRNTLLSLADLEKLVGGEPLTAITNDLFEK
                     LSHNYPLIFICPPEERDRRITISQIHTHKIRGADIILIAEPSQELALAVEGVPMGIEK
                     YWSKYLPLPQSGDPCIFVFSAIIALQYLAYRMSVRKMQWLDSLGIQNHGVHPDAPKNV
                     SKSITVD"
     gene            complement(13628..14473)
                     /locus_tag="PLQ30_04555"
     CDS             complement(13628..14473)
                     /locus_tag="PLQ30_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPW01748.1"
                     /translation="MKGIIVAAGYGTRFLPATKTVPKELLPIGITPSIAFIVKEFVDS
                     GIQDIIIISSRRKKALEDYFDREIELEALFEREARQDQLRKILPFDAHITFVRQMEMR
                     GTGHALMQCANLLDGEFSIVAYPDDIVLGPKPLARQLIDMYEETGKCVLATILETGDV
                     SRYGVIDPAPDGVGVRGFIEKPEKGKEPSHEVSIGRYLYTPRFFEWLEEGWKHHGSGE
                     YYHTYALQKMISEGKVAFSRVQGQRLDTGEPAGYFEAQLHNALNDPSLKKVLAHFVEE
                     HKNEL"
     gene            14698..15933
                     /locus_tag="PLQ30_04560"
     CDS             14698..15933
                     /locus_tag="PLQ30_04560"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW01749.1"
                     /translation="MNALKRYFDVYRGLSQSLYILFFATIINSMGMFVFPFLTLYLTG
                     RLAMTQEKAGFFIMMLNLVYGPASLVGGKLADSFGRKRLMVFAQVLSGLCYIPCGFPS
                     VGSNIRWFLLASVFFDGLTDPARSAMMTDLTSPENRRPAFSLIYLGHNLGFAVGMLLA
                     GFLFENYTSWLFWGNAIAIFVAITLVGLKVTETKPTRGQIEKSFGTGRQDEAHRGNII
                     QALLSRPNLIIFTMLTGLYGFVYAQHRFILPLQTKEFFGASGSVIYGTLMTLNAAMVI
                     VLSTPIMTITQHWKPINAVALSGLLYALGFGFIGLRPSIMLIYITTAIWTLGEIVLAT
                     NEGTYVANSTPISHRARFQAFLPLLGGIGLTISPPIVGSFTDHFGLKMAWPLLGAIAA
                     VASLSIYYLGVMEEKANKK"
     gene            complement(16052..16123)
                     /locus_tag="PLQ30_04565"
     tRNA            complement(16052..16123)
                     /locus_tag="PLQ30_04565"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16089..16091),aa:Val,seq:cac)
     gene            16198..16851
                     /locus_tag="PLQ30_04570"
     CDS             16198..16851
                     /locus_tag="PLQ30_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC59/DGK1/VTE5 family protein"
                     /protein_id="HPW01750.1"
                     /translation="MNKNQKESSSDSSKPGLPHINIRFQSPIDELRIELTRKSLHLLI
                     LFCIPLLDISPMLPVLALIAGMLLYSVSEILRHRGFNVPIISSITERAARRRDSNRFV
                     WGPITLALGATFSLLIFDPLQAKVAILALGLGDSFSSLVGKSFGRIPMPFSGGKSVEG
                     SILCFAIVLLAVYLLTGRFIPSMIIALATTFAEFWPTKDWDNILIPVAAGIIASLVL"
BASE COUNT         4437 a         3950 c         3741 g         4782 t
ORIGIN      
        1 aaacgccagc aagcctggcg atatctttga tactggtcat atatttcatc atagcctctg
       61 aaaagatttt catctagaga cagagctttt gtccgtgcga caaatttgcg caagggtaat
      121 tgcctcgcca tctttgacct ctgccctgaa ggctctcatt gacgaaaact gaaggaatat
      181 cgcacaatat agcatcatga tagtagaaaa acgcctttta tccctttaca tatgtctctt
      241 tgctgtcttt gccctttttg gtatttccat gacagtcata ggggctacgc ttccaaagat
      301 attcgcggaa tttggctggt cttatatgac ggcaggaatg gtcatagcgg cgggttcggt
      361 tggatatttt ctatcgagtt accttgctgg aattctcttc cccttcctgg ggctccgctt
      421 tttgatttcc ttatcgctga tccttatatg ctgtgggctt gaattcttcg caaagacacc
      481 tacagcatgg ctcaatacga tgctctattt tataattgga gtaggccagg gtggaataga
      541 aattgcagtc gactgggctg tactccgcat ggaaggtcct gggggtggaa aagctatgag
      601 cctgatgcat ggtgcttttt cgattggagc cgtagtcgga ccactagtaa tagctatatt
      661 gatcagtgcc aatctggaat ggacgctcgt ttatcgaatt atgggtgtat tattccttgg
      721 tattgcgatt ttcatgcaat tcatgccttt taagttgctc ggaaagggca gaggacagat
      781 aacaaataga tcgaggcgcg atctttttca tcagccgatg tattggcttg gattttttgc
      841 cttgtttgtc tatgtggggg ttgaactagg tatttcgaac tggattgctg agtattttgt
      901 aagtgttttc ggagctccca tacccacagg ttccttcatg gtctctctat tctgggcagg
      961 gctgctaatg ggacgttttg gaattccaat aattcagaaa tccatcaaaa gggaaaggat
     1021 cttgatagct ctatcgacac tgatggccat ttctttgata gctttcaata tgatcggctt
     1081 tattggagat ggaaagcttg ccagtgttat tgccggaatt ttggttatcc ttgctggctt
     1141 tggttgctct attatctatc caactatcat gagtatcatt ggtgattatt tccttcatgc
     1201 tcagagtgag gctgttggtt ttgcggcaat gggtggtggc ataggtggat ttttatttcc
     1261 atatatgatg tcggctgtct caacaggatg gggaattcgc atagggttcg caatctatgc
     1321 tctcttttcg cttcttatgg tcgttcttaa tatcttcctt gtacgagctc aagagcaaaa
     1381 aagtatcggt gtatcggagc cctgatctat ataggaatag gataggaatc gtagcatgaa
     1441 ggcatttcat gcgcttataa agggcgaagt ccaaggcgtt ggatttcgta tgtccgcagt
     1501 gattcgtgct gaacgattag ggttaaaagg ctgggtacga aataccgagg atggtgatgt
     1561 agaagtatgg gcggagggag atcctcaagc tctggagcaa ttctattcct ggcttcaggt
     1621 gggacctgca gctgctcgcg tcgatcgagt cctaaagacc gatgagatac caaagggcaa
     1681 ctatattaga ttttcggtta gcttctgatt attgatgttc agagcctttg atatagctct
     1741 atttccccag gggcatgtct taggtctagg ctacaacagt atgtcccggt ttagaataca
     1801 tcaaagattt tgaacgctgc tgagatattg caaaagcccg ctaagaagca tctctatcgc
     1861 aatcgttgtc agcaccaagc ccataaggcg ttcgattgcc gagagagccc ttgggccaag
     1921 ccactccttc aatttgttcg acatgaaaaa cgctaccatg gtgacaagaa tggctataaa
     1981 aatcgcccca acccattcaa gcgctcgcga agtatcggac ttcatcaaaa gcatgacata
     2041 ggtaatggcg cttggtcccg ctatcaatgg aatggcgacg ggcacaataa atggctcgcc
     2101 ctcgatctca tcctcgacaa gcttcccgcc ggttcctggg aagatcatgt tcaaagcaat
     2161 caagaaaagg attaccccgc ctgatatgcg aaggatatcg tctgatagat tcaaagcgct
     2221 tagaaatacc tcaccaaaga aaagaaatcc caccaagacc agaaaagcga tgagacactc
     2281 ccgtaagatc acttccgaac gccgtccctt aggtacatgc ttcaaggatg caatgaatag
     2341 tggaatattc cctaaaggat cggtgatcag tatgaggagg attgtgacgg aaagaaaact
     2401 ctgcctcata gtccttagct tccgcctcct tgatttatat tcttagtctt tttagctatt
     2461 ataaatagct tgcagctcaa ctagttagca gcgcaattat ctgcttcata tgactttctc
     2521 gttatgcagc ttcatgtagg ttcgcttcag gttcctccgt ccatgctcga gggtcgcgca
     2581 cttctggaga cactaaatat cgaagatata actacagcgg aaagaataat cactccccct
     2641 aagatagcca ttctcgatgg cttttcgccg atagcgagat atacccatac tggatttagc
     2701 gctggttcga ttatagatat gagagaactc tggattgcgg ttatgcgctt tattgcataa
     2761 ctgaaaaata cggcggaaaa acctatttgg actatgccaa ggccgagaat ggcagccaca
     2821 gatctcttgc tgatgacagg cggaggcaag aagatacaaa tcaaaagtga aacgatagct
     2881 gtcatgcaat gggagataaa cagtgattca atgggcgagc cttccttctg ttgtcgcata
     2941 aaaagcatat ggattgcaaa ggtcatacct gccaagaccg ccgctacatc gccctttata
     3001 tggccaaggc tgagcgaatc ggcaaaaaac aagcacatcc ctattacaat cgcaatgagc
     3061 gcacctatct gctctggaag aggcttttct tttaacataa cacccgaaag gagcattaca
     3121 tatactggcg caccatattg aaggagaatt gcatttgcgc tggttgtatt cttgtttgcg
     3181 tagacgaata ggagcatagt gcatgaataa gatatcgcgg cgataatctg gttcttaccg
     3241 aagttcaatt ttggcttgcg tactaccgca aaaataaaga gtgctgcaac aagacttcgc
     3301 gctccggcaa ttgcaatagg atgccaatcg atgagcttaa tgaataagcc gcttagactc
     3361 cagcaaacag aggtcgccac catggccatt atgcctttta tatcgcttct catagtaatc
     3421 ttcgtacaaa agccagtctg agctgatgtt tcaaacctta atccaaatcc cagtttctta
     3481 gatttggagc ttcgatctcc ttgaaataac gtactgtgac tacacgcaac tcatttgtag
     3541 catcttcgtc acagaccacg atggcttttg ggtgcagctg aatgcaactt attgtccaca
     3601 tatggttcac gcccatctcg atgccatgtt ttagcgctct tgccttttcc attcccgata
     3661 caagaatcat gacttcctgt gcatccatta ttgtacctac gcctacggta agagcctgtg
     3721 acgggacttt ttctggatct ccaccaaaga agcgggcatt ggctttcttt gtatcccggg
     3781 tgagtgtctt gatccttgta cgcgatgata gagatgaacc aggttcgttg aacgcgatgt
     3841 gtccatccgc acctaagccg ccgacaaata aatcaattcc accagcatcg catatcgcct
     3901 tttcgtaggc agtgcactct ttctgcaggt ctatggccat gccattgaga atatgtacat
     3961 tttttggttc tatatcgata tgagaaaaaa aattatccca cataaaacga tgatagctct
     4021 gctcatgttc ggacgtaagg ccaacatatt cgtccatgtt gaatgtaatt acattcttaa
     4081 agctgacttt tttagctttg cagagggtga tcagttctct atacattcca agcggagttg
     4141 agcctgttgg aagtccaagt acaaatggct tctgagaatc atgttcattt atgtgctgta
     4201 ccagataata agctgcccat tttgacatca attcatagct ctgctgaatg acaactctca
     4261 tattattcgc ctttcaatag cattatgatc atattttatt ttgccatcaa ttattgtcat
     4321 ctgcacatcg aagtccgcat ttgcgagcac aagatccgcc ttatatcctg ggctaatcat
     4381 accacgtctt ctaaggccga atattcgagc cggatttgca cttgccatct ttactgcgct
     4441 ctcaagcggc actccgaatc tgaccaggtt caggacacct tcgatcatgg taagagaact
     4501 gccagcaatc actccatctc ttgctctata gaagagattg tcttcaaggt atacttcctc
     4561 atcattggca aacatgggag catttaccgc ttgctttgtc ggtttgagcg agtctgtaac
     4621 cagcacgata ttgccttccg gtttgtctcg gaaaagaagg cgaatcagat caggatgcac
     4681 atgcagccca tccgctataa tctcgcaaga taaatccgac tggataagga cggctccgac
     4741 tgcgttaggg tttctatgat gaagccgact catcgcattg aacatgtgtg tggagtgcct
     4801 tattccggct tgcatgcctt ctatcatgtt ttcgtagcta gcatcggtat ggcccgcctg
     4861 aagcacaatg cccaactgat tgcagaacag cgccagctcg cgcattccct tgagctccgg
     4921 cgctacggtc atgctgacaa tatgaccttc cgaagcatcc caaaggcgct gcattagctc
     4981 cagatccaca ggcctgacaa actctattct ctgtacccca agttgagcgg gtgagatgaa
     5041 cgggccttct atatgaattc caataatctc ggctcctgtt tcatgaccca tcgccaagac
     5101 cacggctctt attgcccgga tcatatcttc ttccggcatg ggatacaatg tcggaacaaa
     5161 agagctcact ccccaagcag gtagtatctc gctcattgca agaatggcct cggcggacat
     5221 gtcttctgtg ccaaagcctc caattccatg aatatgggtg tctataaagc cgggagtaag
     5281 ataggcgccc ccggcatcga tcaaatgcac ctgtggccca aaggactttt gcgcaaatct
     5341 gcgctcagag aaaacatcgg cgattgaatc gccctccaca agcacagcac tgcggtccat
     5401 gcgcgcatag cccgcaatga cagtcgcatt gtgaatgcaa aagaccgatt gcatagtctc
     5461 tttttgcaaa tatcagttca gttgctcgtc ccagaagccg tattcgaatc ttcaggggga
     5521 gtggactgct cggaggacga agcactatca ctctcttgga gctgttctat aatctgtttc
     5581 cattctgtga ggagctgttc aatagtctgg aagacaggat cttctttatc atatacggca
     5641 ctaaagcgct tctctgtttc ctccatgtcg acaatggcta gcttgagatc atcagctgac
     5701 cgagctgtct gtgcatacat aagtgctcta tagaagaaca aggtccccat tgcttgatca
     5761 tcgctcgtat acttttcaag cgagcggttt agataatcga agcccgaaac cgggtctgaa
     5821 gcaccaagcc aggtagatag cagcgattgt acgacattgg gatcgacgtt gaatttttcg
     5881 gtaacgctgt ccgcagagac attggcttct tctatttcgc ccattattac tgcgacaaga
     5941 tgctgaatca gcattggggc atattcatcc ggaaagcgct cgatcatcgc atcgacatat
     6001 aaatcagtct tctgcgtatc accagaattg aggccggcct tgataccttc cacataggca
     6061 ttatacgtgg aactagtgac atcaggatct tcgattacct tatcgataac agaataaact
     6121 tggtcgaatt tgccctggta gttgagactg tttgcaaggc ttaccgcaaa gctaagctcc
     6181 tcgggaaata gagaaatagc ttgtcttagt accttttcgg caccttcata ctgctgaaga
     6241 ttcattagaa gttcggcgtg atttgcaaga ctcttgtcgt tatataggcc ataagcgaga
     6301 gctctatcgt agttttctac cgccttttgt tgaatagtat agccatcgat catccaatta
     6361 tcgctatatt gctgatttac ggcgtaaagg taacctgcta attcaaggga gagaatgggt
     6421 ggtatagctt cgctggattt ttctagcgct tcgactagat ctagaggatt gaaatccact
     6481 attgtctgct tttcgggtgg gttcaaaaat gcttcataga tgctctgccc ttccgctaaa
     6541 tcgacgaagc taaaactgac tagcatacca ttgccaatgc tgtcttccag ggctattcga
     6601 atcttctcgg cgagaacata accatctttg ctgtcagggt cgaaggcagc aagtagattc
     6661 caggcttttg aatattgacc tttggcttcc aggagacgag cagcatcgat gatttctttc
     6721 gtctgaggcg cagcatctga atagggtgac tggacggctg gagcttgctg cgcaccgata
     6781 gaagcaatca cagcgagcca tgcaaataat aagattacaa agcgtttcat gaataagact
     6841 ccttcccatt gagggatata ttctctcgta ctatatgaga gaggatactt atctttgtcc
     6901 taaaaagcaa cggtgcgctg caatgcttac aatgctttgc tatgctttac aattgaacac
     6961 catacctaca ccataccttg gttataggct tgcgcctttg ctttctagag attagtcttc
     7021 gattgaaatg agtcgcagat atgttgtggt tgctggcaac atcggtgcag gaaaatcgac
     7081 acttgtgagc ctgctcgccg agcatctagg atttatagct tatcttgagc cagtcctgga
     7141 aaatcctttt ctcaaggatt tctatgcgaa tatgtcacga tgggcattcc atagccagct
     7201 tttctttctg tctcatcgag ctgatatgca ccgcaaattg agcgaagatc cgcattccgt
     7261 tgttcaagac agatcgatct acgaggatgc cgaaatcttt gccaggaatc tttatatcca
     7321 gggaatcatg gccgagcgcg aatggtgtat ctataaagcc ctatatgcaa cccttgccga
     7381 aatcttgcct cacccaagcc ttgtgatata catccgtgct tcggtatcta ctctgcagtc
     7441 gagaataaag cagcgcgggc gagatttcga atcatcgatt cccgattcgt atctcgaagg
     7501 actcaacaat ctctatgagg aatggatttc ctcttttact cttgccccaa ttcttactat
     7561 ccccggagac tccctcgatt ttgtgactga atcgcgggat ctgcaggtaa tcctaagaac
     7621 cataacaaaa cgtctcgaag ataatcaacc ttatctcttt ccttatgaaa tgtgaggtga
     7681 tttacgtggt gatttatgct ttgtcacatt acataattta tattatttct cgttgctatt
     7741 catttctgac atagcaagca aagactctcg ttcaaggcgc cacagagttc ccaaaggcct
     7801 cattgctccc aacgctcgaa gatagaaaaa catctctgtg taactttgtc cattatattt
     7861 tcccatgttc gatagagcgt cagatgtgca ttagctccaa tgcgtgccag agcttctcta
     7921 tcggaaattc ggcagtccat ccatgataag cagttcatcc gtgataatgg gtagtgttcg
     7981 cactagaaac taaaggttct tttcaagtat atgctgccct acaaagaggc ctatgaggta
     8041 ttcttctgaa aaccaaaata accggctcca ccagctcgat agggagcaat atctgccgga
     8101 agatcgagat ccaccacatt tcctactgca tcatacggac ctatgtaacc gtactcacga
     8161 atgctaagaa catggttccg ctgccatatc atcaaatagg tgtgcaatga acaacatgat
     8221 ctttgcctgg atttcttatt tgtgcagatc agttttcctc tcgaaaacag attttattga
     8281 ctttggctta tcgctggcat taatctaata atagagaggt gcgatatggc aaatatgctc
     8341 ttttctattg tattggcctt ttcgctttca gcgatgacag gttatgctca gggctggcca
     8401 gaaggaaaag aatccgattt cttcgataca ctgaaaggca aattggagat agtctttctt
     8461 ggccatggct ctttgcttct agcctacaac ggctctaata tctatataga tccggtgagt
     8521 caatatgcta atttctccaa gtaccccaag gccgacttaa tcttaataac acacgagcac
     8581 aacgaccatc tcgatccaaa agctatagct gctctcagca agcctgaaac acgcataatt
     8641 ctgtcggagg catcccgcaa aaagcttggt catggggaag ttctacagca tggccactcg
     8701 atcgatgttg caggagtaac cgtgctcgca gtacctgcct ataacatcac ttcgtcccgg
     8761 atgggctatc atccaagaga aagaaaagac aatggttatg ttctgagtat tggttcactg
     8821 cgtatctaca tagcaggaga tacggagccg atacccgaaa tggcaaatat cggtaaaatt
     8881 gatattgcat ttttgccaat gaatctgccc tttaccatga caccaacgca ggttgctcaa
     8941 gctgcccgaa tcatcaagcc caggattctc tatccttacc atttcggttc gaccgataca
     9001 agcgagataa caagattgct ctccagcgat aaagatatcg aagtaagaat ccgaaatctt
     9061 aaataacaga gcagctattc ttaccttgcc gataatatat gattttcgat atatttattt
     9121 catatagcta tctataggag tataagaatg gcaaatatca cgtttaaagg aaatccaatc
     9181 caaacaatag ggtctcttcc tgccaaagga gccacggcct cggattttag attgaccaaa
     9241 tctgacctca gcgatgtaag cctagaggat tttgaaggga agatcaaaat cctcaatata
     9301 gttcctagtc ttgacaccgg cgtctgtgcc gccagtgccc gagcattcaa caaggctgca
     9361 tcttctattg atgatgtagt cattttgaca atcagtaggg acctgccttt cgcacagaag
     9421 cgcttttgcg aagccgaagg aatagataag gttatcaccc tatccgaact gcgaacaaga
     9481 aattttggca aggcttatgg tgtcgagatt atctctggtc ctatggctgg ccttttatcg
     9541 cgagcggtag ttgtgctcga caaagccaat aaggttatat acacggaaca agtgccggaa
     9601 attgctcagg agccaaacta taaagctgct ttggaggccg ctcgtactgc tacgaacctt
     9661 gtgtataagc attgagaatg gcataagcaa gccttcttat gcatatgcat aagcatagcc
     9721 aggtacatga tctgctttct tcttatatat acagcacaag tctatgcaga acattggaca
     9781 cattccttgt aaagcacacc gattctctgc gatgatccgc aatgggcttt ctaagctctc
     9841 ttctaagctc tctatcgaga agggaaagtc tacatagtta gctccttgct cttcccttat
     9901 tggctctatt tcctgcttct tacttcaact tattatacac aaggttcgta gcagtaatca
     9961 tagcctgtgc tgcaagaatc ccatcgcaag caagtgctga aagccaagag gagtgtgggg
    10021 ctttaatctt ttctgcctgc ttcaatgcat tttcggatac agagttgctt atagtatgaa
    10081 tgggctttat ccatacgctt atttttcctc cttttagaat ctcgcgtaca aaatcgactt
    10141 tgattcgaca gatagtctct ccatctgcgc aaggcaccgc atcgcatagc caatttatga
    10201 agtgagcatt attgacatgg ccattttgat cgatgtgccg tgtgcgagcc tctaaagcaa
    10261 agacctcgga aaaaccattt agcaggagag tattcttagg ttccgcatat ggatcttttt
    10321 tatcttgcat tgagtggtta gtcgcttgag gaaatgctcc aaaagaagca tcctcgacgc
    10381 caggagagag atccacaaat ggtagagggt aatccgtggt cagatcgatt ggaagcgttc
    10441 tctccggtcg aaccacacgc cgagtcttca tgtccacgat gatatattcg tacagggcac
    10501 ctgcatatct aaagcccgat tcatccagaa gttcgagaca cctctgtgca tagatttttt
    10561 ttgctccgac aggccaagtt ctggcgataa ccctttgccc agttctaggc attgcttcga
    10621 tacggatgtc gatgcgcgag agcatccatg taagccctct agcccacata tcctccatgc
    10681 cgatgcccaa aaccgttgca tgaactcccg ctgtctcctg aaaaaaattg gcaagtgcaa
    10741 agggcttaag aggtccgcca taaccgcagt catatcctct cacttcataa tgaatttcaa
    10801 gaggttttat catcgatgca tcctgatcct ttcctggtct tctttattca ctaatcacca
    10861 aatagcgttg atatccttct gacatttcat atactacctc gttgcatgtt tctaagaaag
    10921 gcattatgga gattccgaag ccttttgctt cttatgaata tgctatgata gatgggctct
    10981 aataatgaac tccgacaaat tgactccgca gccatggaat atcatggtat aatacaaagc
    11041 tatgtgcggt attgtttcac ttgtctatag agatgaaaac ccttcaatag gaaaagaagc
    11101 agaggcgctt ctcaagcgtc tcgagtatcg aggttacgat tcgactggtg ctgcctttat
    11161 aggtgctgat aaacgcatct tgctgcgaaa aaaagttggt gctccttcca gagtctgtcc
    11221 cctcctgcag atcgactcct ttagtggtca gcgattcatt ggacaggtgc gctgggctac
    11281 atatggaagc gttaccgatg tcaatagtca gcctcatcat gtgcattgca aaatggagct
    11341 agttggagcc cataatggca atatctcgaa taccgatacg ctcaaaacct ggctttccgc
    11401 ccgcggccat gcggtaattt cggacaacga tggagagata attgtgcatc tcattgaaga
    11461 gcactattct gccgcccaat cgctggccag ctctgacctt gcctatcttc gaaaggctta
    11521 tgcgaatgcc ggtcttgcag aaggcgtgcc cgatggagtt ctgcgcatga tcgaggcaat
    11581 tcgcagagct caagcgcttg cccaaggttc ctatgccgca gccatcgctg atcctcagct
    11641 gccgggagta ttcgccatta agtctggctc ttcgctctat gccggcgttg gcagtgactc
    11701 ttttggcaat ttcatcgttc tttcttcgga tctaacaagt gtgctttcta agacgcgcac
    11761 gcttatccct ctttccgaag gagagggaat ttggtttacc gataaggaat acctcgtatt
    11821 caaccttcag ggcaacccct ttttctcaca tcctcgcctc aagagatcaa agctctcgat
    11881 caaagatacc cagctctcac ccgaatacca gcactatatg gagcaggaaa tctcatcgag
    11941 catgaacaac atcaaccaga ttctacgtta ttatttccga gattcctcta ccgaacctct
    12001 ttcagagctt tttgaagacc ataaggatga ttgcaaggaa gccgcggaga aaatttcttc
    12061 gctctctgaa cgttttggaa atgaggctct ggtttcagga atgaaggaga tttttgaatc
    12121 tccagcctgg aacgaaatag aagcaagagt tcagaataca ggtgcagcgc actttgtcgg
    12181 tcaagatttt atctcggatg agacagagct gctcaaagag cttgctgccc tcaatccgcc
    12241 tatgcgaggg aagcttctgc tcatggacaa ggtaatcgta tggcgaaagc gccgcgcggt
    12301 tctgcgatat acgggtgagc tcaagaatgc tatccgcgaa accacacagt caggaggcag
    12361 aatttacttc atcgcatccg gcacatcgta tcatgcagct cttattgccg cttatttttt
    12421 caattcaaca gcacatatcc cggtctatcc ctgcaatcct ggcatgtttc gctcaatgta
    12481 tttcaattgt atggggaaaa acgatctcat tatcggaata tctcagtcgg gcgaaacaaa
    12541 agatctagtc gacatatttc aagatgtaaa gaaaaccaac aattcggtgc gtcatgtcag
    12601 catagtgaat aatgagaata gccgaatacc tcaagagctt tcggatttct atctgccgct
    12661 cttgtgtgga ccggagatag ctgtagcagc aacgaaatcc tttacgagcc agattgccat
    12721 cctctacctt ttagccgcaa gcttctctat gccagagcat aaagttcgag agaacctgat
    12781 caaggccaga gacctgatga ccgagactat cgagaccagg atgggcgata tcgagagtat
    12841 agccgcacac ctctttatgg cttcttctat tcatatcctg ggaacaggac tcatcgggct
    12901 agcaaaagaa ggcgctttaa agatacgcga aatagtccta aatcacacgg aaggctatga
    12961 tgccgcagag ttcaagcatg gccccaatac tatccttggc aggaacacat tgctttctct
    13021 agcagacctg gaaaagcttg tcggaggcga gccattgact gccattacaa atgatctctt
    13081 cgaaaagctt tcgcataact atccgctcat attcatctgt ccgccagaag agcgcgatag
    13141 gcgtatcaca ataagccaga tacacactca caaaatcaga ggagcggata ttattctcat
    13201 cgcagagcct tcgcaagagc tggctctcgc agtggaaggt gtgccgatgg gcatcgagaa
    13261 atattggtct aagtatcttc ccctgcctca atctggcgat ccctgcatct ttgtattctc
    13321 ggctataatc gcgctgcaat atcttgctta ccgtatgtcg gtgcgcaaaa tgcaatggct
    13381 tgattcactg ggaatccaaa atcacggcgt acatcccgac gcacctaaga atgtctccaa
    13441 gtcgattaca gtggactaat cgaaagtggc tataggccga gacagttggt cggaaaaatc
    13501 ttgctagaat tcgactccca ataagcacag agggcggttt taataagcgc aagcaagcat
    13561 taggccttga tagacagtag caggcattat gtgcacggac aggaacaggc ttgttatgag
    13621 gggatgatca aagctcattc ttatgttcct ctacaaaatg ggctagtact ttcttgagag
    13681 aagggtcatt aagggcgtta tgaagctgag cctcgaaata tccagctggt tcaccggtgt
    13741 ctagtctttg accttggact cgcgaaaaag cgaccttgcc ctcgctgatc atcttttgaa
    13801 gcgcatatgt atggtagtac tcgcctgaac catggtgctt ccagccctcc tcgagccatt
    13861 cgaagaatct cggtgtatag aggtaacgcc caatcgagac ttcatggctt ggctctttgc
    13921 ctttttcagg tttttctata aagcctcgaa ctcccacacc atccggagct gggtctatta
    13981 caccatagcg cgacacatcg ccagtctcca atatcgtggc aagaacgcat ttccctgtct
    14041 cttcgtacat atcgatgagc tggcgagcta ggggctttgg ccccagcaca atatcatcgg
    14101 gataggctac aatggaaaac tccccatcga gaagattagc acattgcatc agagcatggc
    14161 ctgtgcccct catttccatc tgacggacaa acgttatgtg ggcatcaaaa ggaagaatct
    14221 ttctcaattg atcttgacga gcttctcgtt caaatagcgc ctctagttcg atttctcgat
    14281 cgaaatagtc ttcgagagct ttcttgcgtc tgctcgagat tatgattata tcctgaatcc
    14341 cggaatctac aaattccttg acaataaagg caatactcgg tgttatacca ataggcagaa
    14401 gttctttggg aacggttttg gttgcgggta gaaaacgtgt accatagcct gctgcaacga
    14461 ttattccttt catgagagct attgtaaaat atgattgaaa tcatgacaat tctatctgaa
    14521 tcggaattgt ccttattctt gaatgtaatc cagataaaaa ataaaccatt gggcatcgct
    14581 gctgtggcat ttgcagaaaa ctcttgttct acgcttaagc ctttcgaggc aattcaggag
    14641 gctcataata ggctgccctt gacctcttca tcttaagcca tgtaatgttt gctatttatg
    14701 aatgcattaa agcgctattt cgatgtttat cgagggctct cgcaatctct ctatattctc
    14761 ttctttgcta caataataaa ttccatgggc atgtttgtgt ttccttttct tactttgtat
    14821 ctgactggac ggcttgcaat gactcaagag aaggctggtt ttttcattat gatgctcaac
    14881 ctggtctatg gtcctgcttc tcttgtcggt ggaaagcttg ccgatagctt tgggcgaaaa
    14941 agattgatgg tatttgcaca ggtcttgtcg ggcttgtgct atattccatg tggtttccca
    15001 tccgttggta gcaatattcg ctggtttttg cttgcaagtg ttttctttga cgggttgact
    15061 gatccagccc gctcagcaat gatgactgat ctcacttccc ccgaaaacag acgcccggct
    15121 ttttctctga tttatctagg tcacaatctg ggctttgcag taggaatgct ccttgctggc
    15181 tttctatttg aaaactacac atcctggctt ttctggggaa atgcaatcgc aatattcgta
    15241 gccataacac tggtcggact aaaggttacg gagacaaagc ctactcgcgg gcagatcgaa
    15301 aagagctttg gtacaggcag gcaagatgaa gctcatagag gcaatattat ccaggccctt
    15361 ctttcaaggc ctaatctaat catattcact atgctgacgg ggctctatgg ctttgtctat
    15421 gcacaacatc gatttatctt gccacttcag accaaggaat tctttggtgc tagcggttca
    15481 gttatctatg gcactttgat gacactcaat gcagcgatgg taatcgtgct atccacgcca
    15541 atcatgacaa tcacccagca ttggaagccc ataaatgcgg tagccctctc cggcttattg
    15601 tatgctcttg gattcggatt tattggcctt agaccttcca taatgcttat ctacataacc
    15661 acggcaatct ggactttggg cgaaattgtc cttgcgacaa acgagggtac ctatgtcgca
    15721 aatagtactc caatttcgca cagggcaaga tttcaagcat ttcttccact tctaggcgga
    15781 atcggcttga ccattagccc acccatcgtt ggttctttta ccgaccattt tggactaaag
    15841 atggcctggc ccctccttgg agctatagcg gctgttgcga gtttgtctat ttattatctg
    15901 ggagtgatgg aagaaaaagc caataagaaa taatcgttca aacatgagcc cttggtaagc
    15961 gcttgcaatg attgatttct gtatatcata acttcataga attaacgatt ttctctcgtg
    16021 caattcatgc cgatacaaaa agagtgctgt atgggcgata ttggattcga accaatgact
    16081 tccaccgtgt gaaggtggca ctctcccgct gagttaatcg cccaatcaac actcttctgc
    16141 ggcatcctaa caaagcatgc cgcaatatgt caaggatagt cgaggaagcg agctaaagtg
    16201 aacaaaaacc agaaagaatc ctcttctgat tcttctaaac ctgggctccc tcatatcaat
    16261 attcgatttc aatctccaat cgatgaactg cgcatagaac tcacgcgaaa atctctccat
    16321 ctcctcattt tgttttgtat acctcttctc gatatttcgc ccatgctacc agtcttagca
    16381 ttgatagccg gaatgctcct ctattccgta agtgagattc taagacaccg gggcttcaat
    16441 gtacctataa tctcgtcgat cacagaaaga gccgcacgcc gcagggatag caatcgcttc
    16501 gtatggggcc caataacact ggcgctggga gcgactttct ctctgctcat ttttgatcct
    16561 cttcaagcca aagtggctat actcgcgctc ggccttggcg acagcttctc gagcctggtg
    16621 ggtaagtctt tcggtcgcat tccgatgccg ttttccggag gaaagtcggt agaaggatcg
    16681 atattgtgtt tcgccatagt cttgcttgcc gtttatctgc ttacaggtcg ctttatacca
    16741 tctatgatca ttgcattggc aacgaccttt gcggaatttt ggcctacaaa ggactgggac
    16801 aatattctta ttccggttgc tgcgggaata attgcttctc tggtactttg attagtgcta
    16861 cgaaccttgt ataagaagtt gaagcaagat gaagagatga gggcgataaa
//
LOCUS       DAOMZL010000037        16385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_3188_length_16385_cov_16.421433, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000037 DAOMZL010000000
VERSION     DAOMZL010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 16385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16385
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3188_length_16385_cov_16.421433"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..784)
                     /locus_tag="PLQ30_04575"
     CDS             complement(77..784)
                     /locus_tag="PLQ30_04575"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HPW01751.1"
                     /translation="MSYCPECGVEIGNAKRCPLCGTLNPKACEKENAVVYSVNDQKNP
                     EEKTLPQEKPTILWEILSVTLAIAILVLGAIDLFESRRFSWSLYAIVSILFLWVELTA
                     FFMLRKQPVLRILLSVLAPPLLFVIIGYISGNQRWALGLAVPIIMLVESLGGLVYLIF
                     EKSKKKGLNLIAYITIVAAMFCIGLEVLIDLFAIGMVVLRWSPVCAIALLSIAIFFLY
                     LHFRVFKNADLHRLFHF"
     gene            complement(807..2183)
                     /locus_tag="PLQ30_04580"
     CDS             complement(807..2183)
                     /locus_tag="PLQ30_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01752.1"
                     /translation="MNKNRQSTVSHRFVHTRREDNAGEWYALDNAAIIMPAVTDDVST
                     SLFRFEAELDAPIDPEILKKALALTVNRMPYFNVRLRRGFFWYYFEPCKTMPPIYPDL
                     DFPCQGWNINRHGTRMFRILWKRNRISGEFSHALTDGTGGIGFFKSLLANYLILKGVD
                     PGARLGEGEWKDIAVSSGEMSGLAISEEFEDAYQRYFPNGLPFPDLRPHAFHLSKSCL
                     PKGQYRIISGKLLTSEVIAEAHRRNISLTELMSAVYLDALQHLWYATNPPPREPYVSV
                     EIPINLRQLFPSRTFRNFSLFVLISEDMRLGMRDFDELVLRTHYQMKLEYDRMSISRH
                     LCRNAGSARNLAVRLVPLRIKDFFARVFFSKFGESMLSGFISNLGQLKMPPGFVPHIL
                     SFGFIPAPSMVTMTNASMLSWGNELIINFGSLAQSRELEQLFFRRLRSIGLHVSVSCR
                     SDNGISED"
     gene            complement(2191..2886)
                     /locus_tag="PLQ30_04585"
     CDS             complement(2191..2886)
                     /locus_tag="PLQ30_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HPW01753.1"
                     /translation="MIATLINAAAIILGSLIGLLIRKGIKDEYRKVVFTAAGLTSLAI
                     GIQMVLETDHILSFALALMVGGLLGTAFDVEGAIERLGDGLKKRFARRSEDTFATGFL
                     NASVLFCAGAMTIVGSFNAGTVGDYSLLLTKSVLDGFVSIIFAGAMGIGVAFAALSVF
                     VYQGALTLLSEYIKPYVSDLMLGELTAIGGALVIMIALGLLEIKSFKTGNFLPAMAVI
                     IILVLAMPFITFL"
     gene            2945..3355
                     /locus_tag="PLQ30_04590"
     CDS             2945..3355
                     /locus_tag="PLQ30_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01754.1"
                     /translation="MHKSLLSSVALCTLSLVGILVFSCASSPPPVPEDASAQKIIQLA
                     QERYDVYDLNGAKYYYQVLLERYGSDPNYMLNAKYEIAFIEYKKGNKDIAIAGFKEII
                     ERYNEPDASSLSPTWKVLSQMMIEKLEGNKNIQD"
     gene            complement(3405..5387)
                     /locus_tag="PLQ30_04595"
     CDS             complement(3405..5387)
                     /locus_tag="PLQ30_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPW01755.1"
                     /translation="MPFLQLSGIRLSFGARDLILNASLNLQDGSRAALTGPNGAGKST
                     LMKIAVGLVKPDAGEVIATKDTRIAYLPQTGVQFESGTVYEIAEAAFAFDHDLIKEQE
                     EIGRLLDSSHLSEKETKRLIARHHEITEKLEQAEYWNRGERISEVLNGLDFKIKDFGR
                     SASELSGGWQMRLALAKILLEEPDIMLLDEPTNYLDLEARTWLEDFLKAYPGAVLVVS
                     HDRYFLDVTVREVYELFNGCLTRYVGTYSQYEMRRSQELEALFEAWERQQEERQRIED
                     FIRRFRYKDSKAAQVQSRIKMLEKMTPIEIPEGMKRIHFSFPPAPRSPRVVLRIHQLS
                     RSYGELRVIEPFSLEVERGQKLALVGPNGAGKSTLMRIIAKEDNPTSGEAILGANVLV
                     GYFSQESAEIMESTATVKREAESVCPPEMLPKIRNLLGAFLFRDDDIDKPISVLSGGE
                     RSRLALLKLLLKPANLLVLDEPTNHLDLTSKDILLEALSRFEGTVILVSHDRQFLDEL
                     PDRILELSCGNRPLLYYGNYAYYLTKKAEKENAKLEAMQEDNKKPKQRWQSETDSASS
                     VMDQELIPDKNWEAEKARKARLRKLKRREEDISTRIEEIQTEKEKLQEELAQPRNYIN
                     GEKTRRILAELEDLDRETEALNEEWLEIAQSLSEEA"
     gene            complement(5400..6905)
                     /locus_tag="PLQ30_04600"
     CDS             complement(5400..6905)
                     /locus_tag="PLQ30_04600"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPW01756.1"
                     /translation="MVYYSIFVPTKNTSKNLLLIVHSRCMNFEERRDQGVSKGVFSIV
                     GLVLILFCILLASCARLEGWGLVIWSVKGTTAKAGSIVPVYLKSNISKTYMIGLPDDS
                     KTKIEVPFATIEFFASKKAAEKRAREFAPYALYYLSAGRDGLPVRDKPSVTGRRVYRL
                     MIGESVKVLNRVEGEAVFTGGKALPGEWYFVQAADGTRGYVFSNTMILYEEKEGVSVP
                     VLTTAPTPSVSLLDMIYAKPWRPAYFQLMLDEDTVDPDLFLLQYGLFADAKNSQVRIE
                     LPGYSKAFSFGSVSQIGDWLVFEGSNLRIKFENESTIVADWSGLEPMLPEEGWTPGGQ
                     AARFVRFDASIPVIISGENSRRDSELKVFFQRVSQLQTNGSSSSATFLSDHAGILTID
                     LKGNFEWSHIDQLPAGFVPEVSLGSGSGSDGNVLKGRIRFGLHLETQLSSYWMGGFSL
                     FMGEEPQRYDFLYRFENEQLIMAKAIQVSLRGTTESIDSRFSPVSFYLISK"
     gene            6906..8492
                     /locus_tag="PLQ30_04605"
     CDS             6906..8492
                     /locus_tag="PLQ30_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPW01757.1"
                     /translation="MSETPSQKSLELKENYVNSPGIHAAIEMGSTGIRLIIAEIDSKG
                     DLKILDRASKQSRIGRDVFTMGSISRDGMRETIAILDSFSELLAGYDLHPSDIQVMGT
                     SALREASNRDTFMDRINLQTGFRVRIIEDIEENHLMYLAVKKALEDERSYLSRSNAMI
                     LEVGGGTTELMILRKGQMVSSHSLSIGTLRIDEQIRESAQQPRQFIEMYLESNIRTAC
                     DILAEDLQLDSLRIFVLIGADARFAANCLSEKVLGNYAVLDRKQFIEFADYVANMSTE
                     ECMAQYHLSWNEVENYTAGLTIERMFLERTGADTIIVPNISIREGILLAQVQGMNKKI
                     EEELRRQVVASARALGKRFHYDEAHAAHVRNISLKIFDALKKEHGLGIHERMLLEISA
                     ILHDIGTYLRTSGHHKHSEYIVANSEIFGLNMDDINIVSNVVRYHRKSQPLSTHVNFI
                     ALPRESRIIVLKLAAILRVADALDRSHLGRIKELSFERTDDRFIIRTSESLDFSLEKL
                     SLAEKGDMFEDVFGLIPVLI"
     gene            8526..10706
                     /gene="ppk1"
                     /locus_tag="PLQ30_04610"
     CDS             8526..10706
                     /gene="ppk1"
                     /locus_tag="PLQ30_04610"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HPW01758.1"
                     /translation="MKDLPMLNRELSWLDYNARVLQEGLKGSLPLLEQLNFMCIFASN
                     LDEFFMVRVAAVKTAVQTGQTGQTNEEWAGMEPRALLEAIHEKVQASYDKLHMHLHGT
                     ILPSLVQHGIQFVQEKDWTTKESRYLERLFAERIYPLLTPLRLEDNHFPSTGSLQVHV
                     AFRLEENDSVAIIQVPKNIDRFIILPQYLSLEPDNSANSMVIAAFRVALLEDVILAFG
                     SKLFAGLKISSGLIFKVNRDADFSVDEDRDDDFLTAMEEVLTGRQNSTPVRMVYSGED
                     QVLIDALRQSLSLDALDLYQADWLIDLGRVDELCNPEFYKAHGFTPDRVLFFQPWKPV
                     QLFEEKGSIFDWIDEHDRFVNLPYESFDVVQRFIEESASDPSVLGIKMTLYRTSGMNS
                     PIVHALAKAARSGKQVVVLLELKARFDEEKNISWATELEQAGAIVTYGVARLKVHAKA
                     ALIIRKRQDGSIARYIHLSTGNYNEKTARNYVDFCLFTANPSLCTDIALFFNILTGYS
                     NIQSLSLIALSPFDLKDRIIGFIEREAAQTTPESPGFIIAKLNSLSDPDLIAALYRAS
                     AKGVQIKLNVRGVCTLIPGMAGLSENIEVCSVLGRYLEHGRMLYFRNGGSEELYISSA
                     DWLQRNMKKRLELLFPVLDKEIKARCKDILNIYFADNTHAYRLKNDGTWMDLSLSRRP
                     EDPLLYAQEKLHKMTKKTVRNLEEKQEILQVRRSKGKSIGQSIP"
     gene            10750..11034
                     /locus_tag="PLQ30_04615"
     CDS             10750..11034
                     /locus_tag="PLQ30_04615"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPW01759.1"
                     /translation="MKATKETLDHIRKKKAYFVNKPNPLMMRHALRTLGTTYEETIIV
                     GDRMDTDIIAGIESQIETILVLTGVTDEKDMPKFAYAPTFVLESVSEIPQ"
     gene            complement(11064..12566)
                     /locus_tag="PLQ30_04620"
     CDS             complement(11064..12566)
                     /locus_tag="PLQ30_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPW01760.1"
                     /translation="MIHESSSIYQIFLRNFTKEGTFCAAIPELKRIADMGFEWIYLTP
                     IHPIGEKARKGTLGSPYAIKDYRKINPELGDENDFAAFVQEVHAHGLKLMIDVVFNHT
                     SPDSVLVHEHPDWFLRAADGLPWRKCENWSDVVDFDYRSSPHLWLELIDTLTIWRDRG
                     VDGFRCDVASLVPSEFWRQARQRVNQYDPGIKEERAQLLWLAESVHPAFLRKMRLLGY
                     NSWSEPELHVAAFDLTYDYDGWERLEKIWEQGMPFERYLEYLYVQETLYPKGAKKLRF
                     LENHDQERAAHRFGNGAKLRAMTPFYQFLPGVAMSYMGQELALEHMPSLFEKDPIDLE
                     RGDKEFERYFIASLRATKEAKKEAPFFSWLIKGDAALQDITDQDMASRDKAPRTRTVQ
                     NRTSNNCAVREEVIYCLRSVEPAISPYLIDRSMIIKGNYLLVIQPELGTSGPKSSGVK
                     SQRRISFAEESMPRALFDIEGTDLLSGKEVRIAEGDILPRIPASLIRLKD"
     gene            12806..13525
                     /locus_tag="PLQ30_04625"
     CDS             12806..13525
                     /locus_tag="PLQ30_04625"
                     /inference="COORDINATES: protein motif:HMM:NF037435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HPW01761.1"
                     /translation="MEKKENTEVLDDTFIAAARDYRMLLDKDYPIDASLKLVGDRYRL
                     SHNGRMMLYRGILSLKASQINLAKLVRSPWERASLGVDGYNILFTLMNYMHGHPMFIA
                     TDGFLRDVGGAHGRIADHRGFERMAEITAEYLAALNLQSLTFFLDAPVSSSGRQAGFL
                     REAFAQRSINAEVRLENGVDHFLAYWEGELIATADSAIIGRTNAHVFDLARAILENKF
                     DARFISLARFLEPSYPISAPP"
     gene            complement(13509..14174)
                     /locus_tag="PLQ30_04630"
     CDS             complement(13509..14174)
                     /locus_tag="PLQ30_04630"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HPW01762.1"
                     /translation="MSEILRLLIRPLGETILMIAASTLISTILGGGLGILLFTLSQDR
                     FAGKYPGALLVKRIADRAINMFRSFPYVILMVLVLPLSRLIVGTSLGTIASIVPLSIA
                     AIPFLARIVESSLSEVDSGVIDAAISCAASKTRILFAILIPEALPSLISGLTLTMISL
                     LGYSAMAGVIGGGGLGDLAIRYGYQRFRADVMAVAVVAIVALVELIQWTGSMIIKRIK
                     AAR"
     gene            complement(14167..15069)
                     /locus_tag="PLQ30_04635"
     CDS             complement(14167..15069)
                     /locus_tag="PLQ30_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW01763.1"
                     /translation="MFHAKRPEEAADQALVAHLSHQTSHRGGSISPKGAFPKDIDDSH
                     TKKTELAIYIDEVSKSFGNHSVLHKVSLAVPRGSIHGIMGKSGAGKTTLIRIAGLLEK
                     PDQGSIWYEGIGMPVHELHGRAMLEARRQVGFVFQSFNLFSSRSASGNIAFPLEAAGW
                     PREKIRARVEELLEFVELSDKANRPAGRLSGGEKQRVAIARALANSPSMLLSDEATSA
                     LDPETTRAVLDLIRSLRDRLNLTVIMVTHQKEVIRRICDTVSVISGGSIVEQGSVAEV
                     FSNPRSEAAKAFLKEEEEKGHSYV"
     gene            complement(15402..15818)
                     /locus_tag="PLQ30_04640"
     CDS             complement(15402..15818)
                     /locus_tag="PLQ30_04640"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HPW01764.1"
                     /translation="MRERAIVKEIKGDMVTVSIELQEGCAVCVNNGRCKIRKSDLSVY
                     NKDHIDIDEGDRVVVEIRTSEQAKSAFWVLGFPLIMLFVGYGFGAFIFKGSGEGPAVV
                     CAGLGFIFAMGIGMLIQYRRKLQSFPYILGKEGEIL"
     gene            complement(15787..>16385)
                     /locus_tag="PLQ30_04645"
     CDS             complement(15787..>16385)
                     /locus_tag="PLQ30_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HPW01765.1"
                     /translation="TEFYPKAGWVLRPANLPEGDPGRSWRIFEKNGKRVAVVMLLGQS
                     GFARIHANNPFLAIDQLSNFLHKETACITVNFHAATTAEKLSMARHTNGKVSAMLGSG
                     GKVLTADARILSKKTATITDLGRTGSMLSVGGLDPDAKVKEFLTGIPSWSREASAQPE
                     AQGTYICFDDDGKAISIEPFRICGKEIVDEGKSDSQRN"
BASE COUNT         4501 a         3995 c         3610 g         4279 t
ORIGIN      
        1 tcggctgcat catactgacc taacttagct ttgtttcgtc aaatccgcct tgcttgccaa
       61 attgaagcat gaagcatcaa aaatgaaaaa ggcgatgcaa atccgcattc ttgaaaacgc
      121 gaaagtgaag gtataagaag aagattgcga tggataagag agcaatggcg cagacaggag
      181 accatcgcag aaccaccata ccaatcgcaa ataaatcgat tagcacttcc aggccaatgc
      241 aaaacatcgc cgccactatt gttatataag caattagatt gagacctttc ttcttgctct
      301 tttcgaatat aaggtaaacc aagccaccta gagattctac aagcattatg attggaaccg
      361 ccagaccgag cgcccatcgc tggttaccag aaatgtaccc aattattaca aataataagg
      421 gtggtgcaag aacgctcaat aagatacgaa gaacgggctg ctttctcaac atgaaaaatg
      481 ctgtcaattc cacccataga aacaaaatgg atacaatagc gtagagagac cacgagaatc
      541 gcctggattc aaaaagatca atagcgccaa gcaccagtat tgctatagcc aatgtcacag
      601 agagaatttc ccaaagaatc gtcggttttt cttggggcaa agttttttct tcggggtttt
      661 tttgatcatt taccgagtag acaactgcat tttccttttc gcaagccttt gggtttaagg
      721 ttccgcagag tgggcatcgt ttggcgttgc cgatttctac cccgcattcg ggacaatacg
      781 acatgattca ccccccattt ttgccttcaa tcttcactga ttccattgtc agatcgacat
      841 gaaacactga catgcagtcc gatacttctc aatcgtcgaa aaaacaattg ctcgagttcg
      901 cgcgattgcg ccaagcttcc aaaattgata ataagttcat tgccccaact tagcattgag
      961 gcatttgtca tggtaaccat gcttggagcc ggtataaatc caaagctcaa tatatgagga
     1021 acaaaacctg gaggcatttt caattggccc aagttagaaa tgaaaccaga aagcatactc
     1081 tcgccaaatt tcgagaaaaa gaccctggcg aaaaaatcct ttattctaag aggaaccaat
     1141 cggacagcca gattccttgc acttccggca ttacggcaca agtgtctaga tatgctcatt
     1201 cgatcatatt cgagcttcat ctgatagtga gtccgcaaaa caagctcatc gaaatctctc
     1261 ataccgagcc gcatatcttc actgatcagc acaaagagag aaaaattgcg aaaggttcga
     1321 gaaggaaaaa gctggcgcag attgataggt atctctactg agacataggg ctcacgcgga
     1381 gggggattag ttgcgtacca taaatgctgc aaggcatcga gatagacggc tgacatgagt
     1441 tcggtcaagc taatatttct tcgatgagcc tcagcgatga cttcgctggt aagcaacttc
     1501 cctgaaatta tgcggtactg gcctttgggc aagcaggatt tgctcaaatg aaaagcatgc
     1561 ggtcttagat ccgggaatgg caatccattg ggaaaatacc gctgataagc atcttcgaat
     1621 tcttcgctta tcgccaagcc actcatttca cctgaggaca cagctatgtc tttccactcg
     1681 ccttctccta gtcgtgcacc cgggtcgaca cccttcaata tgaggtagtt tgcaaggaga
     1741 gacttaaaaa aacctatgcc tcccgtacca tcagtcaaag catgagaaaa ctcacccgaa
     1801 attctgttcc gcttccaaag aatgcgaaac atgcgcgtcc catggcgatt tatattccat
     1861 ccctgacaag gaaagtccaa atccggataa atcggcggca ttgttttgca aggctcaaaa
     1921 taataccaga aaaatcctct tcggagcctt acattgaaat aaggcatacg attcactgta
     1981 agagcaagcg cctttttcaa tatttctgga tctatgggcg catcgagctc ggcttcgaac
     2041 ctaaaaagac tcgtgctgac gtcatctgtg actgctggca tgatgattgc agcgttgtcg
     2101 agcgcatacc attcgccagc attatcttcg cgccgagtgt ggacgaatct atggcttact
     2161 gtgctctggc gatttttgtt catgtttcga ttatagaaat gtaataaatg gcattgcaag
     2221 gacgagaatt ataatgacgg ccattgccgg aagaaaatta ccggttttga agcttttgat
     2281 ctccaagagc cccagggcta tcatgattac aagcgctcct ccgatagccg taagctcgcc
     2341 caacattagg tcactgacat agggctttat gtactcggat agtaaagtaa gcgctccctg
     2401 atacacaaaa acagaaagag ccgcaaatgc tacgccaatt cccatagccc cagcgaatat
     2461 aattgagaca aaaccatcaa gcacgctctt tgtcaggaga agcgaataat cccctacggt
     2521 tcccgcattg aatgaaccca ctattgtcat ggcgcccgca cagaacaaaa ccgaagcatt
     2581 gagaaatcct gttgcgaaag tgtcttcgga cctgcgcgca aagcgttttt taagaccatc
     2641 tcctagcctc tctatcgcac cttcgacatc gaaggcagta cccagaagtc cacctaccat
     2701 caatgcaagg gcaaaagaaa gaatatggtc tgtctctagc accatctgaa taccaatagc
     2761 aagcgatgta agccctgccg cggtaaacac aacctttcga tattcgtctt ttatgccttt
     2821 tcgtataaga agcccaatga gtgaacccag aatgatggcc gctgcattga ttaatgttgc
     2881 gatcatggct tgtcttgacc tcgtttggcg agtatagcat aattgccact tgtgaggaaa
     2941 tgacatgcat aaatcgcttt taagctctgt agccttatgt acgctctcac tcgtcggaat
     3001 tctggtattt tcttgtgcct cttcgcctcc accggtgcct gaagatgcaa gtgctcaaaa
     3061 gatcatccag ctagcccagg aacgctacga tgtctatgat ctcaatggcg ctaagtatta
     3121 ttatcaggtg ctgttggaaa gatatggcag cgatccaaac tatatgctca atgccaagta
     3181 tgagatagct ttcattgaat acaagaaagg caataaggat atagcgattg cgggttttaa
     3241 agaaataatc gaacgctata atgagcccga tgcatcgagt ctttccccaa catggaaagt
     3301 gctctcccag atgatgatcg aaaagctaga aggcaataag aatattcaag attaatagct
     3361 atagaccgcg ctttttccaa ttcaaaggag cttatttcaa gcctttaagc ttcctcggat
     3421 aaagactgcg caatctcgag ccattcttca tttagagctt cggtttctct atcgaggtct
     3481 tccaattctg ccaggatgcg ccgcgtcttt tctccattta tatagtttcg aggctgagcc
     3541 aattcttcct ggagtttctc tttttctgtt tgaatctcct caatgcgagt agaaatatct
     3601 tcttctcgtc gttttagctt gcgtaatcgc gctttgcggg ctttttccgc ttcccaattc
     3661 ttatccggaa ttagctcctg atccatgacg gaagaagcag aatctgtctc actttgccag
     3721 cgctgtttgg gcttcttatt atcttcttgc atcgcttcta attttgcatt ctctttttcg
     3781 gccttcttgg tcaaatagta ggcataattc ccataataga gaagcggtct attgccgcaa
     3841 gagagttcca gaatccgatc aggaagttca tcgaggaatt gacgatcatg agagacaaga
     3901 attaccgtgc cttcaaatct acttagggct tcgagcaaaa tgtctttact agtcaaatcc
     3961 agatgattag tcggctcatc tagcaccaga aggtttgcgg gctttagaag caacttgaga
     4021 agagccaacc gcgagcgttc tcctccagat aacactgaga ttggcttatc aatatcgtca
     4081 tcgcggaaaa gaaaagctcc tagcagattg cgaatctttg gaagcatttc gggtggacag
     4141 acggattcgg cttctctttt taccgttgcg gtcgattcca tgatctccgc tgattcctgt
     4201 gaaaaataac ccacgagcac attggctcct agaattgctt ctccgcttgt tggattatct
     4261 tcctttgcaa ttatgcgcat cagcgtagat ttccctgcgc catttggacc tacaagggcc
     4321 aatttttgtc cacgctcaac ttcgagagaa aatggttcta ttaccctgag ttctccataa
     4381 gacctggaga gctgatgaat tcgcaagacc acacgagggg atcttggtgc tggcggaaaa
     4441 gagaagtgga tccgcttcat accctccggg atctctatag gtgtcatctt ttccaacatt
     4501 tttatacggc tctgtacctg agcagccttg gaatccttgt atctaaaacg ccgtatgaaa
     4561 tcttcgattc tttgcctttc ttcttgttgg cgctcccagg cttcgaagag tgcttccaat
     4621 tcttgagatc tgcgcatttc atactggcta taggtcccca catagcgtgt aaggcaacca
     4681 ttgaatagct catatacttc ccgtacagta acatcgagaa agtatctatc atgtgagact
     4741 actaataccg caccagggta ggctttaaga aaatcttcca gccaggttct agcctctagg
     4801 tctaaataat tggttggctc atccagcagc atgatatctg gctcttctag caatatcttt
     4861 gccagcgcaa gtcgcatctg ccatccacct gatagctcag acgcagaacg tccgaaatcc
     4921 ttgatcttga aatctagccc attcaaaact tcagaaatcc tctcccctcg attccagtat
     4981 tctgcctgct cgagtttctc agtgatttca tgatgccttg caataaggcg ttttgtttct
     5041 ttttcgctta aatgagagga atctagtagt cgccctattt cttcctgttc ctttataaga
     5101 tcatgatcaa acgcaaatgc ggcttcggcg atctcataga cagttcctga ttcgaattga
     5161 actcctgtct gtggaaggta ggctatgcgg gtatcctttg tggcgatgac ttcccctgca
     5221 tcaggcttga caagtcccac agctattttc ataagtgtcg acttgccagc accgttgggt
     5281 ccggtcaatg ccgcacgaga cccgtcctgc agattcaagc tcgcatttaa tatgagatct
     5341 ctggcaccga atgatagcct aattccgctc agctgcagaa agggcatgct attacaacct
     5401 tatttactta taagataaaa gcttacagga gagaaccttg aatcgatact ctccgtagtt
     5461 cctcttaggc tcacctgaat agccttggcc atgataagct gttcattttc gaagcgatat
     5521 aagaagtcat atctctgggg ttcttcaccc ataaacagtg aaaaaccacc catccaatag
     5581 gaagaaagct gagtctctaa gtggaggcca aatcgaattc tgccttttaa aacattgccg
     5641 tctgaccccg agcctgatcc cagcgagact tcggggacaa aaccagcagg aagctggtcg
     5701 atatgactcc attcgaagtt ccctttgagg tctatagtca gaatccccgc atggtcagag
     5761 aggaaggtcg ctgaagaaga gctgccgttt gtctgaagct gtgagactct ctggaaaaag
     5821 accttcaatt ctgaatcgcg gcgtgagttc tctcctgaga taataaccgg aatcgatgca
     5881 tcgaagcgca cgaaacgagc cgcctgtcca ccaggagtcc agccttcttc ggggagcata
     5941 ggctctagac cactccaatc cgctacaatt gtcgattcgt tttcgaactt gattctgaga
     6001 tttgatcctt cgaatacaag ccaatctcct atctggctga ctgaaccgaa actaaatgcc
     6061 ttagaatagc caggaagttc tattctaacc tgagaattct ttgcgtccgc aaaaaggcca
     6121 tattgtagca ggaaaaggtc gggatccacg gtgtcttcat ctagcatgag ctgaaaataa
     6181 gccggacgcc aaggttttgc gtatatcata tcgagcaatg agacacttgg agtcggagcc
     6241 gttgttagta ccggcacact tacgccctct ttttcctcat ataaaatcat ggtattgctg
     6301 aatacatagc ctcgggtgcc atcggcagct tgcacaaaat accattctcc ggggagcgct
     6361 tttccaccgg tgaatacggc ttcgccttcc actctgttga gaactttcac cgattcgcca
     6421 atcatcagcc gatagactct tctgccggtc acgcttggct tgtcgcgcac aggaagacca
     6481 tctcgccccg cgctcaggta atatagagca taaggtgcaa attcccttgc cctcttttcc
     6541 gcggcttttt tcgaagcaaa aaactcaatc gttgcaaatg gcacttctat ttttgtcttg
     6601 ctatcgtccg gcaagccgat catataggtt ttgcttatgt ttgatttcag gtaaactggg
     6661 acgatagagc ctgctttggc cgttgttccc tttacggacc agatgaccaa accccatcct
     6721 tccaatctcg cacaactggc cagcaaaatg cagaataaaa tgagaacaag gccaacaatc
     6781 gaaaaaaccc ctttactgac accctgatct cttctttctt cgaaattcat acagcgagag
     6841 tgtacaatga gcaaaagatt cttgctagtg tttttggtcg gcacaaagat gctataataa
     6901 accatatgtc cgagacgcca tctcaaaaat cgctagaact caaggaaaac tacgtaaact
     6961 cgcctggaat ccatgctgct attgaaatgg gttccaccgg tatacgcctc ataatcgccg
     7021 aaatcgattc gaaaggagac ctgaagatac tcgatagagc atccaaacaa tctcgtattg
     7081 gtcgcgatgt gtttacaatg ggcagcattt ctcgggatgg catgagagaa accattgcta
     7141 tacttgactc attcagtgag cttctagcgg gttatgatct gcatccttcg gatattcagg
     7201 taatgggaac gagcgcgctc cgagaagcct cgaacagaga cacttttatg gatcgcataa
     7261 atctgcagac agggttccgt gttcggatca ttgaagacat cgaagagaat catcttatgt
     7321 acctggcagt aaaaaaggcg ctcgaggacg aaaggtccta tcttagtcgc tcgaatgcga
     7381 tgattctgga ggtagggggt ggcactacag agcttatgat tctaagaaag ggccaaatgg
     7441 tttccagcca tagccttagc ataggcactc ttagaataga tgaacagatc cgcgaatcag
     7501 cacagcaacc caggcaattc atagaaatgt atctcgaaag caatataaga acagcctgcg
     7561 atatcttggc cgaagatttg cagctcgatt ctttgcgcat attcgttctt attggtgcgg
     7621 acgcgcgctt tgccgcaaac tgcctttcag aaaaagtcct gggcaactac gcggtactgg
     7681 accgcaaaca gttcatcgaa tttgcagact atgttgccaa catgtccacc gaggaatgca
     7741 tggcacaata tcacctttca tggaatgaag tcgaaaacta taccgcaggg ctcaccattg
     7801 aacgcatgtt tcttgaaaga acaggagcag atacgattat cgtacccaat atctctattc
     7861 gcgaaggaat tctgcttgcg caggtccagg gaatgaataa aaagatcgaa gaggaactac
     7921 gcagacaggt agtagcatcg gctcgtgctc ttggcaaacg cttccattac gatgaggctc
     7981 atgccgctca tgtaaggaac atcagtctta agatattcga tgcccttaaa aaggaacatg
     8041 gccttggcat tcatgagaga atgttgctcg aaatctcagc gattcttcat gatataggga
     8101 cttacctaag aacaagtgga caccataaac acagcgaata tatagttgcc aactccgaga
     8161 tcttcggtct caatatggat gatatcaaca tagtatctaa tgtagttcgc tatcatcgaa
     8221 aatctcagcc gctttcgacc cacgtcaatt tcatcgccct tccgcgagaa agccgtataa
     8281 tagtgctcaa attggcagcc attctgcgcg tcgcggatgc gctcgatcga agtcacctgg
     8341 gccgtatcaa ggagctgtct tttgaacgaa cggatgatag atttataatt cgaacttcgg
     8401 agtcgctgga tttctcgctg gaaaaactct ctctcgcgga gaaaggcgat atgttcgaag
     8461 atgtctttgg gctcatacct gttttaatct aagacatatt gatgtgagca caaatagaga
     8521 agagtatgaa agaccttccg atgctaaacc gtgagctttc gtggctggat tacaatgccc
     8581 gtgttttgca ggaaggactc aaaggctctt tgcctctcct cgaacagctc aattttatgt
     8641 gtatttttgc atccaatctg gatgaattct tcatggttcg tgtcgctgcc gtcaagacag
     8701 ccgtacagac ggggcagaca ggccagacga atgaagaatg ggctggtatg gagcctcgtg
     8761 cgcttctgga ggctattcat gagaaagttc aagccagcta tgataagctc catatgcatt
     8821 tgcatggcac gattctcccc agtctggttc aacacggtat tcaatttgtt caagaaaaag
     8881 actggacaac caaagaaagc agatatctcg aaaggctttt tgcggaaagg atatatcctc
     8941 tccttactcc cttgcggcta gaagataacc atttcccatc gacaggcagt cttcaggtcc
     9001 atgtcgcatt tagactcgaa gagaatgact ctgtggctat tattcaagtg cccaagaata
     9061 tcgaccgttt catcattctg cctcaatatc taagtcttga gccggataat tctgcaaatt
     9121 cgatggttat tgcagccttt cgcgttgctc tcctggaaga tgtgattctg gcatttggaa
     9181 gtaaactctt tgccggccta aaaatatcga gtggccttat attcaaggta aacagagatg
     9241 cagatttcag cgtggatgaa gatagggatg atgatttctt gaccgccatg gaggaagtat
     9301 tgaccgggcg gcaaaattct acgcctgtgc ggatggttta ttccggagaa gatcaggttc
     9361 tcatagatgc cctgaggcaa agcctctcgc tcgatgccct ggatctatat caggccgact
     9421 ggcttataga cctgggaaga gtcgatgagc tctgcaatcc agaattctac aaggctcatg
     9481 gctttacacc ggatagagtg ctattcttcc agccctggaa acctgttcag ctattcgaag
     9541 aaaagggcag catcttcgat tggatcgatg aacatgaccg attcgtaaat ctgccttatg
     9601 aatccttcga tgtggtccag cgcttcatag aagaaagcgc tagcgatcca tcggttctgg
     9661 gaataaaaat gacactctat agaacaagtg gcatgaattc gcctattgtg catgcccttg
     9721 caaaggccgc gcgaagtgga aagcaagttg ttgttctcct cgagctcaag gcgcgattcg
     9781 atgaagaaaa gaatatctca tgggctaccg agcttgaaca ggcaggagca atcgttacct
     9841 acggagtcgc tcggcttaag gtacatgcaa aagcagcttt gatcatcaga aaacgacagg
     9901 atggaagtat tgcgcgatat attcacctat cgaccggcaa ttacaacgaa aagaccgcgc
     9961 gaaattatgt cgatttctgt ctattcaccg caaaccctag tctatgcacg gacattgctc
    10021 tttttttcaa cattctaact ggctattcga acatacaatc gctctctcta atcgccctta
    10081 gccccttcga tctcaaagac cgcatcatcg gatttataga aagagaagcc gcccaaacca
    10141 ctccggaatc gccgggtttt ataattgcaa agctcaattc tttatcggac cctgacttga
    10201 tagcggctct ctaccgggct tctgccaagg gagtacagat aaagctcaat gtgagggggg
    10261 tttgtacgct gatccccggg atggcagggc tttccgaaaa catcgaggtc tgctcggtct
    10321 taggacgcta tctcgagcat ggccgaatgc tgtatttccg aaacggtggc tcggaagaac
    10381 tatatatctc cagcgccgac tggctccagc gaaacatgaa gaaaagactg gagttgcttt
    10441 tcccggtgct agataaggaa atcaaagcac gatgtaagga catcttgaat atctattttg
    10501 cagacaacac acatgcctac aggctcaaaa atgatggcac ctggatggac ctttctctct
    10561 ctcgacgtcc ggaagatccg cttttatatg cacaggagaa acttcataag atgaccaaaa
    10621 agacggttcg aaaccttgaa gaaaagcagg aaatccttca ggtgaggcga tcgaaaggga
    10681 agtctatcgg ccaatcgatt ccatgatgtt gtctttgata gtaatatcaa aattgcaggt
    10741 acggaagaaa tgaaagcaac aaaagagact ttggatcata tccgaaagaa aaaggcatac
    10801 tttgtcaaca aacccaatcc gctcatgatg aggcatgcgc tccgaactct gggaacaaca
    10861 tatgaggaga caattattgt aggcgacaga atggataccg acataatagc aggcatagag
    10921 tctcaaatag aaactatact cgtcctaaca ggcgttaccg atgagaaaga tatgcctaaa
    10981 ttcgcctatg cacccacttt tgtgctcgag agtgtctcgg aaataccaca atgattcttt
    11041 cacaatgaga catatttgga gtatcaatcc tttaggcgaa tgaggctggc gggtattctt
    11101 ggtagtatgt cgccctcggc aattcgcact tcttttccag aaagcagatc cgttccttcg
    11161 atgtcaaaga gagcgcgagg catagattcc tctgcaaacg aaattctcct ttgagattta
    11221 acaccgctgg attttggccc agaagtaccc aattcaggct gtatcacaag aagataattc
    11281 ccttttatta tcatggatcg atcgatcaaa taaggactta ttgcgggttc gacactcctt
    11341 agacaataga tgacttcttc tcttacggcg caattgttcg atgtgcggtt ctggacggtt
    11401 cgggttctcg gcgctttatc tcggctggcc atgtcttggt cagtaatatc ttgaagcgcg
    11461 gcatcaccct taattaacca tgaaaaaaaa ggggcttcct tttttgcttc ttttgtagcc
    11521 cgcaggctcg caataaaata gcgctcgaac tctttatctc cacgctctaa atctatagga
    11581 tccttttcga aaagactggg catatgctct aaagccaatt cctgccccat atagctcatc
    11641 gctactccag gcaagaattg ataaaaagga gtcattgcgc gcagcttcgc tccatttcca
    11701 aatctatgag ccgcacgctc ttgatcatgg ttttcaagaa acctgagctt ttttgcccct
    11761 tttggataga gggtctcctg aacgtagaga tattccaagt atcgctcaaa gggcatccct
    11821 tgctcccata ttttctcaag cctttcccaa ccatcgtaat cataagtcag gtcgaaagca
    11881 gctacatgca actcaggctc actccatgaa ttgtacccaa gcaatcgcat tttccttaag
    11941 aaagctggat gaacgctctc cgcgagccaa agaagctggg ctctctcctc cttaattccg
    12001 ggatcgtatt gatttacgcg ttgtcgagcc tgtctccaaa actcgctcgg gacaagcgat
    12061 gccacatcac aacgaaaacc gtccactccc ctatctctcc aaattgtcag tgtgtcgatt
    12121 agctcaagcc agagatgagg cgagctcctg taatcaaaat ctaccacatc gctccaattt
    12181 tcgcattttc tccatggaag cccatccgcg gctctaagaa accagtccgg atgctcgtgc
    12241 acaagcaccg aatcgggtga agtatgattg aatacaacat ctatcatgag cttgagccca
    12301 tgagcatgta cctcttgtac aaaggcagca aaatcatttt cgtctcccag ctcaggattg
    12361 atcttacgat agtcttttat ggcataggga ctgccaagag tccccttgcg agccttttca
    12421 cctataggat gtataggcgt gagatatatc cattcaaaac ccatgtcggc gatgcgcttg
    12481 agctctggta tagctgcgca aaaagttcct tctttagtaa aattacgcaa aaatatctga
    12541 tagatagagc tgctttcatg aatcatcgat agcttctcct tcatatttta ataggcatgt
    12601 aaatagaata aacaataaca gagcgccgca aaaccggcaa ggaaggcgaa agatcatcgg
    12661 cataaataag cgcttgggca gaaagcaggc tgacaggcca aagagtgtag tatatgctta
    12721 ataagtcagg cttggtcata aatcgacctg agcaagaagc tcctttagac catttaggtc
    12781 ctttggatcc tttagagcga gttctatgga aaagaaagag aatactgaag tccttgatga
    12841 tacatttatt gccgctgcaa gggattatcg aatgctactc gataaggact atcccatcga
    12901 tgcaagcctc aaattagtag gggatcgcta taggctttcg cacaatgggc gcatgatgct
    12961 atatagaggt atcctttctc taaaagcttc ccagatcaat ctcgccaagc ttgtccgcag
    13021 tccatgggag agagcttcgc tcggtgtcga tggttacaat atccttttca ctctcatgaa
    13081 ctatatgcat ggtcacccta tgttcattgc tacggacgga ttcttgcgcg atgtgggcgg
    13141 cgcgcatggg cgcatagcgg atcaccgtgg tttcgagcgt atggcggaaa ttacggcaga
    13201 atacctggcc gccctcaatc tccagagtct gactttcttc ctcgacgctc cggtctcttc
    13261 gagcggtcga caggctggat tcctgcgaga agcctttgct cagcggtcta tcaatgccga
    13321 agttcgcttg gaaaatggag tcgatcactt tctggcatat tgggaaggtg agctcatagc
    13381 tacggctgac tcggccataa ttgggcggac caatgctcat gtcttcgacc tcgctcgcgc
    13441 aatactcgaa aataagttcg atgctcgctt tatctctctc gcgcgcttct tggaacccag
    13501 ctaccctatc agcgcgccgc cttgattcgt tttataatca ttgaaccggt ccattggata
    13561 agttccacca gcgcaacgat agccactacc gctacagcca tgacatccgc gcgaaagcgt
    13621 tgatagccgt atctaatagc cagatctcca agcccaccac ctcctataac acctgccatg
    13681 gctgaataac cgagcaaact gatcatcgtt agtgttaggc cggatataag cgatggtagc
    13741 gcctcaggaa ttagaatggc aaataggatg cgcgtctttg aggccgcaca agaaattgct
    13801 gcatcgatta ctcccgaatc tacttcggag agcgatgatt ccacaatgcg cgctagaaaa
    13861 ggaattgcag ctatagacag aggcacaatg gacgcgatag ttccaaggct tgttccaact
    13921 atcaggcggg acagcggcaa gacgagcacc ataaggatga catatggaaa agagcggaac
    13981 atatttattg cacgatcagc tatacgcttt actaagagag ctccaggata cttacccgca
    14041 aagcgatctt gcgaaagagt aaatagcaat attcccaatc cgccaccaag tatagtcgat
    14101 atcaatgtcg aagcggctat catcaggatg gtctctccta agggtcgaat aaggagtcga
    14161 agtatctcag acatagctgt gccccttttc ttcttcttcc ttgagaaaag cctttgccgc
    14221 ttcggaacga ggattggaga agacttccgc cacagagccc tgttcgacga tagatccgcc
    14281 ggagatcacc gatacggtat cgcagatgcg ccgtatgacc tctttctgat gggtaaccat
    14341 gattacggtg aggtttagtc tgtctcgcag agaccgaatc aaatctagca ccgctcttgt
    14401 cgtctccgga tcgagcgcgc tcgttgcttc atcgctcaaa agcatcgaag ggctatttgc
    14461 gagcgcgcga gctattgcca ccctctgctt ttcgccgcca gagagcctgc cagcaggcct
    14521 gttagccttg tccgaaagct cgacgaactc aagaagttcc tctactcgcg cacggatttt
    14581 ttctcgaggc caacccgcgg cttcgagagg aaaggctata ttgcccgatg cgcttcgcga
    14641 agaaaagaga ttaaagcttt gaaacacaaa gccaacctgc cggcgcgcct cgagcattgc
    14701 acgcccatgc agctcatgca caggcattcc gataccttca taccaaattg agccctggtc
    14761 tggcttttcc agaagaccag caatgcggat caaggtagtt ttgcccgctc cactcttgcc
    14821 cataatgcca tggatgcttc ccctgggcac ggcgagcgat accttgtgca gcacgctatg
    14881 gttgccaaaa gacttcgaca cttcatcgat gtagatcgcc agctcggttt tctttgtatg
    14941 agaatcatca atgtccttgg gaaaggcccc tttagggctt atagaccccc cgcgatgcga
    15001 ggtctggtgg gaaagatgcg ctaccagcgc ctgatcagcg gcctcctctg gccgctttgc
    15061 atgaaacatg aaagatcttg tgtctgaatc ataagtgttc ctttaagaca gaacaataga
    15121 ggattttgtc tcttcttgtc aatagataaa ccgcgatgaa aattattggg ctcgaagata
    15181 tgctgcctga ttatcttagc gcaaaattct cgtgaaccca cctccgcata aaattatcat
    15241 gcattacatc aataaagagc gattgccctt tcaagaaatc atagcgcaca cgccttacaa
    15301 aaaatcatgg aacaccttta tcaacagaaa atcagaataa aattagaaga aaatcaaaaa
    15361 gtggattcgc ctatcgttcg gattcatcga ttatcgcttc tctagagaat ttcgccctcc
    15421 tttcctagaa tataaggaaa ggattgtagc tttctgcgat actggatgag cattcctatt
    15481 cccatggcaa agataaaacc caagccggcg caaaccacgg caggtccttc gcccgaaccc
    15541 ttgaagataa aagccccaaa accataacca acaaaaagca tgataagagg aaaacccagg
    15601 acccaaaaag cgctctttgc ttgttcgctt gtgcgaatct cgactactac ccgatctccc
    15661 tcatctatat cgatatgatc cttgttatac acggaaagat cactcttgcg tatcttgcat
    15721 ctaccattgt tgacacacac agcacaacct tcttgcaatt cgatcgaaac tgtcaccata
    15781 tcacctttaa tttctttgac tatcgctctt tccctcatct acgatctcct tgccgcaaat
    15841 gcggaatggt tcaatcgaaa tcgccttccc gtcatcatcg aagcagatat atgttccttg
    15901 tgcttccggc tgagcgctgg cttctcttga ccatgacggt ataccggtca aaaattcctt
    15961 gaccttagca tccggatcaa ggcctcctac agatagcatg ctgccagttc tgcccagatc
    16021 ggtaatagta gccgtttttt tgctgagaat gcgagcatct gctgtgagca ccttgcctcc
    16081 cgaaccaagc atcgcggaga cttttccatt cgtgtgcctt gccatagaca gcttctcagc
    16141 ggtagttgca gcgtgaaaat tgacggttat gcaggcggtc tccttgtgca ggaaattgct
    16201 gagctgatct attgccaaaa aaggattgtt tgcatggatc cttgcaaatc cagactgccc
    16261 aagaagcatg acaaccgcta ctcttttgcc atttttctcg aaaattctcc aactgcgtcc
    16321 gggatcccct tcgggaagat tagccggcct tagaacccat cctgctttag ggtagaattc
    16381 agtca
//
LOCUS       DAOMZL010000038        16378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_3189_length_16378_cov_16.052809, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000038 DAOMZL010000000
VERSION     DAOMZL010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 16378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16378
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3189_length_16378_cov_16.052809"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..2343
                     /gene="ftsH"
                     /locus_tag="PLQ30_04650"
     CDS             304..2343
                     /gene="ftsH"
                     /locus_tag="PLQ30_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPW01766.1"
                     /translation="MMPSKGKNNWKLDQQRKTDKNSNNRKKPGMPEGFQFRFSLGYVL
                     IALLAVSFFNYFLFKSDTTLVPYSAFKEKVRSGEIRRVEIDVNYYTGYTEPSPSEPSS
                     PLPPSAKPGVVYKTVPVSDPEFTSLLDEKGVVYSASPRGGSTILSLLLNWILPFAIMI
                     FLWRTIMSRFMGSNSNVLAFGQNKATVVAEGDIKTRFADVAGVDEAKAELVEVVDFLK
                     NPQKYTEIGGKIPKGVLLVGPPGTGKTLLARAVAGEANVPFFKMSGADFVEMFVGVGA
                     ARVRDLFKQAREKAPCIIFIDEIDALGKSRASGFIGGNDEREQTLNQLLVEMDGFDST
                     SGLIILAATNRPDVLDPALLRAGRFDRQVLVDKPDMKGREEILKIHTKNIKMDPSVDL
                     SKIARSTPGFAGADLANVVNEAALLAVRAGRKKVKEEDFQNAIEKVIAGLEKKNRMIN
                     PKEKQIVAVHETGHAITAALTPGADPVRKISIVPRGYGALGYTLQMPLEDRYLITEEE
                     LLGKIDVLLGGRAAEELVFQSISTGASNDIANATEIAKRMITDYGMSDKFKNVALTRR
                     SSGLISPQQASDPFATKEYSEETQRYIDETIASMINERYRHVVNLLTEKRDLLNRISS
                     VLLEKEVIEESEFAELLEGAGVSMPEAIKEPVAAAESVPLPGSTEKVFISPTPHE"
     gene            2661..3638
                     /locus_tag="PLQ30_04655"
     CDS             2661..3638
                     /locus_tag="PLQ30_04655"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPW01767.1"
                     /translation="MSIVVRSISAYIPSLRITNEELAMRMDTTDEWIRSHTGIGSRHI
                     APDGLQTSDMATEAAKSALDKANISVQELDYIILATATPDYLGFPATACIVQDKIGAY
                     GVASFDVAAGCTGFIYALNIASSMLEASHGRNALVIGAEGLSRFVNWEDRSTAVLFGD
                     GAGAAILSRIDESSRGCLSFILGAEGSGTKDLFLVQPERTKAFERQEPIIPFIYMDGK
                     KVYDFAVKSITVLIQRLLHKTAYKIEDFTWIVPHQANARIVQAAAKRLSIPLDKFYIN
                     IEEYANTSAASIPIALAEMCEKGLLKKNDLIMLVGFGAGLTYGAAVIRW"
     gene            3746..5026
                     /gene="fabF"
                     /locus_tag="PLQ30_04660"
     CDS             3746..5026
                     /gene="fabF"
                     /locus_tag="PLQ30_04660"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPW01768.1"
                     /translation="MQNEVAVTGLGIISPIGNSIDEFWKNCVNGATGIGPITHFDASR
                     LESRIAAEVKNFDPSVWIDRKEARKMALFSQYAVAAAAQAWLDAGLPDFVQNETLENK
                     SDIDPNRIAVILGNGIGGLEVFTGSHAKMLQDGPDRIPPMTIPQMIANEAAANIAMHF
                     GVHGPVLTQVTACSSGTDALGEALDLIRAGRADIVIAGGTEAAITEFAVGSFCRLKAL
                     STSYNERPEVASRPFDRDRDGFVIGEGAGILILERLDYAKARNAKIRAIFAGYGASCD
                     AYHLTAPQPEGVFGVKAIEHALRDADLQSADIEYYNAHGTGTDLNDPMETRMLKKAFG
                     SSVQSLKISSTKSMTGHCIAAAGAIEAIACIKALETGMLPPTIHLDNPDIEQGCDLDY
                     IPNTAQHRKIKTAMSASLGFGGHNGIIIFKAVGA"
     gene            5119..5535
                     /gene="fabZ"
                     /locus_tag="PLQ30_04665"
     CDS             5119..5535
                     /gene="fabZ"
                     /locus_tag="PLQ30_04665"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HPW01769.1"
                     /translation="MNVESYLPHRPPFLFVDDVHIKGDTILAQHTFRDDDWFFKGHFP
                     SFPIVPGVLLVESMAQAGGVGVKMMGIYPKSLFMFAKIKEARFRQPVRPGDTLIIKIK
                     NVRASSVYLHQKGIGKVGDKVAVEAEWISIASGVPE"
     gene            5532..6746
                     /locus_tag="PLQ30_04670"
     CDS             5532..6746
                     /locus_tag="PLQ30_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-2-enoyl-CoA reductase family protein"
                     /protein_id="HPW01770.1"
                     /translation="MIIKPMIRNNICLNAHPEGCRKEVRLQAARVMNFRERLSKSSIN
                     DSSAKPNHALIIGCSTGYGLASRLVAAFGYGAETVGFSYEKEPSATKPATPGWYANKE
                     FDRLAQEKGLFAASFSQIDAFSHTAKQKAIEIALENKFKYDLVIYSLASSVRVDPDTG
                     VLYRSVIKPIGRQYAGRTIDIFTEKFSEASVQPATEEEITQTIKVMGGEDWQLWIDAL
                     KKKDLLAPEALTLAYSYIGPPFSWPIYRDGTIGQAKLHLEETAKLLEKNYGKDNGLRS
                     FVSINKAVVTRASAVIPIIPLYISTLFKVMKERNIHEDCLDQMMRLFSERLYQKRGTG
                     IPTDEEGRIRIDDLEMGPSVQNEISSRLARIDESNLHELADVKGFRSDFLRAHGFDVP
                     GVDYERDIVSFE"
     gene            6743..7522
                     /locus_tag="PLQ30_04675"
     CDS             6743..7522
                     /locus_tag="PLQ30_04675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPW01771.1"
                     /translation="MNILILDSSSETQFLSLKDRRTQHIIEVLKKEVGGRMRAGSLNG
                     LVGWAILESIGNTEIELRFVAEGTAPPLRPLRILIGTVRPIQAARIVKELAILGVESI
                     VFLPTELGEKSYTQSHFYQQKEYLAYAREGAEQSGNPRLPEIGLAWSLDRALIKAERF
                     ESMDHTNTINPESAGEAKINKCTKLLCHPDPYAKLLSEIILSELPVILAIGTERGWTK
                     HEIDIFLNAGYLPCSLGDRILKTETAAFAAVSILLSRLGYM"
     gene            complement(7546..9351)
                     /gene="pepF"
                     /locus_tag="PLQ30_04680"
     CDS             complement(7546..9351)
                     /gene="pepF"
                     /locus_tag="PLQ30_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HPW01772.1"
                     /translation="MAKTIPERSNVPAEHRWNLASLFSDDSEWERELVTFNAMILDAE
                     KSRLSLGADRKAFLEILKAFEEYLILEERLGYYADLRVTEDEGNSIARGMLERYVNLS
                     TQGQAAWSWLSPAIQMLDDDFFDSCLKDSAFENYRVFLSKLRRFKPHVLSEAEERLLA
                     LQSEAAQTPKDAFSVLTNVDFDFGTIEIPEGPKPLTQSTFAYFMRLPDRSVRRKAYLQ
                     FYASFEAHRHTLAALYAGSVKLDKYRAKVRKYPSARAMALFPDNVPEAVYDNLVSTVN
                     KNIPALHHYYKLRKELLGLDSLRHYDVYNPLVQEIKIERSYEQAVNIVIEALRPLGEE
                     YVSTLRRGLLGGWVDRYENKGKRSGAFSAGSFLGDPYILMNYKEDVLDDLFTIAHEGG
                     HSMHSWYSARNNPFMCYGYTIFEAEVASTFNEQLVFSYLYENSKDDAEKAYLLANRID
                     NTLGTLFRQTMFAEFEQRVHEIAESGEAITVDVLRKEYRNLLERYFGPEMILENISDL
                     EGLRIPHFYNAFYVYKYATGLSAGIALSERVLKGGKKEREDYLDFLKSGGSRFPIESL
                     KLAGVDMSTPEPVEAACRRFAEDVEALSAILKARL"
     gene            complement(9393..10412)
                     /locus_tag="PLQ30_04685"
     CDS             complement(9393..10412)
                     /locus_tag="PLQ30_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPW01773.1"
                     /translation="MSKVTIKDIAQRAGVSKTTVSFALNHPDRLSKETYKRIMAIIDE
                     LGYVPNPFARSLTTKRLGAIGLLLPQKIGDIFANPHMTQVISGIGEECERREFSLAIL
                     PLIRGRIIEAARKSYVDGLITIGVGPGHEVVSLLKQNHIPFVTIDGEESASIINIGID
                     QKNAAADLMRHVLKRGHQRLAILALEPDTKPSGSEISSIVVQRRLEGFQAVLKDAGLS
                     ITDTRVFLQNCKGSIQSGYESAYHILSLDQRPTAIVAMSDITAIGAMIAARELGLEIP
                     EDVAVAGFDDIPESVLVSPRLTTIHQPDRDKGAEAARMVINMLNGSSGQHIKLPYQLI
                     VRESA"
     gene            10480..12159
                     /locus_tag="PLQ30_04690"
     CDS             10480..12159
                     /locus_tag="PLQ30_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HPW01774.1"
                     /translation="MNTMTLTIDGYKEISVPVGSSAEEIFAQAGMEREWGTCEHPFPP
                     LAALVNNELTSLSAPIDADCRIAPIYPDSPMGAEVYRHSLCFLLAMAAKNIAPKRRLI
                     VSMAIGNGYYHYFDDEEPVSPSLLESLCLYMREIVAQNLPIRIVLQSWNVVFEYFKHS
                     NQAETFALMEYMNDPFIMLNECQDYRDLHISPLVPRTGLLSVWELIPYRRGILLRFPH
                     TKKPYEMDPFVDIPVLYDIAEEYEQKAKVLNAGSIGALNKINQLGEIQDFVLMAEALQ
                     NKKLASIADIIAEKSDKTRVILIAGPSSSGKTTTAKKLSVQLRALGFKPIHIELDNYF
                     VDRSRTPLDKDGKPDFECLEALDIEFLNRQLLDLFEGKEIELPVFDFKSGIRKPSGTF
                     ISIKENEILILEGIHGLNERLTPQISADNKLKIYVSALTQLNVDDHNRVRTTDYRLLR
                     RMVRDYNFRGHDARQTLAMWSSVQRGERLYIFPFQGDADIAFNSALDYELGVLKVFVE
                     PLLRTVKPHHVEYTDARRIQAFISRISPISPQYVPSDSILREFIGSSVFKY"
     gene            complement(12213..13673)
                     /locus_tag="PLQ30_04695"
     CDS             complement(12213..13673)
                     /locus_tag="PLQ30_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPW01775.1"
                     /translation="MKLNLSLDNLSEAFPEDLTPDQRARAQTIFLKTLSDKTHRFYGG
                     KIQVVPKAGLYGFNWFNVWYTPGVSKISTTIRDDNDQSFALSNRGNLVAVVSDSTRVL
                     GDGDCTPPGGLGVMEGKAFLMKYLGGVDAIALCINSRDGDGKPNPDKIIDFVKMLEPS
                     VGAVNLEDISQPNCFKVLDELREACEIPVWHDDAQGTACVTLAGLLNALELAGKKLNE
                     AKIVLLGAGASNTTIARLILADGGRGENMVIFDSKGGLHRNRRDIEADKRYYRKWELC
                     LQTNPRCIETEEEALKGADVLIALSTPGPDTVKREWIRSMAPKSIVFACANPVPEIWP
                     YAAKEEGAYIVATGRGDFPNQVNNSICFPGLLKGALLVRSRKITDHMAIRCAHSIADF
                     AKSRGISSDNIIATMEETEVFAREAADVAMAAVEEGVARLPDTWDNIYSKAKADILAA
                     RSLTQDLMKEGHIAPIPEEMLQEAFDFALSEVRKKP"
     gene            complement(13852..14838)
                     /locus_tag="PLQ30_04700"
     CDS             complement(13852..14838)
                     /locus_tag="PLQ30_04700"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HPW01776.1"
                     /translation="MADLRTYLMGLQLQNPIIIGASGLTSMPDGVEKAAHSGVGAIVL
                     KSLFEEQILAVLGQEMAGLDLNSHPDAEAFISRTAWEDSTDKYLKLISESKRKVDTVP
                     IIASINCVGQGRWADFAGRIEEAGADALELNIAYVPFSPQISGQEIEKRVLSTVKEAR
                     SVTKLPIMVKLGNHYSSLPYLVHGLSKEGANAAVIFNRFFRFDFNLSTMRLSGAQPLS
                     SETEYHESLRWTSILYKRVGLEIVSSTGIHSADAAIKCLLAGASAVEVCSVIYKQGWG
                     IIGTMLKDMNILMDSLGYSCMDSLQGKLATINADRPEEYHRLQYIKALTDIY"
     gene            complement(14897..>16378)
                     /gene="thrS"
                     /locus_tag="PLQ30_04705"
     CDS             complement(14897..>16378)
                     /gene="thrS"
                     /locus_tag="PLQ30_04705"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPW01777.1"
                     /translation="LFRDQPFKLELIEGLEEGTISIYKQGDFVDLCRGPHVKSTREIR
                     PDAFKLKTIAGAYWRGDESKPMLTRIYAYAFESKAKLEEYLHMLEEAERRDHRKLGKE
                     LDLFSTHEEAGPGLIYWHPKGGRIRVELENWWRDEHYKNGYEILFTPHIGKSWLWETS
                     GHLGFYKANMYAPMKIDEDEYFIKPMNCPFHIMIYKTSSHSYRDLPLRWAELGTVYRY
                     ERSGVLHGLMRVRGFTQDDAHIICTPDQIEDEILEVLRFSLSIWKTLGFKEIKAYLAT
                     RPSDSVGEPERWDQALLSLKKAIDKEGLLYEVDEGGGAFYGPKIDLKVKDAIGREWQM
                     TTIQFDFNEPERFDMVFIDKDGKQKRPYMVHRALLGSLERFFGVFIEHYAGAFPVWLM
                     PDQVAIIPVAPGFEGYAKTVARFLQEKGIRAKALISEERMNAKIRDAQNQRIPYMAIV
                     GQREQESGTVSIRLRTGDQESGIPLEVFAARVLEKIQTKSLDL"
BASE COUNT         4420 a         3852 c         3703 g         4403 t
ORIGIN      
        1 gcactctgtc cccaatgggt tttacggcca taaatatgtc ctcctaataa atgctggatg
       61 atttgtggta gagaaccgag ttgaaatata aaaaaacaat cccttctggt caagcatgtc
      121 catctacttt tgaacagatc gagctaaaag ccacattccg aagccacatt ccaaaatgtg
      181 aaatagaata ttcgccaaat aaaatgaaaa tcattatatt ttcagatagt gaacgtttct
      241 gcttacgaga gatatgcaaa tgctctataa ttaaaatagg ccatcgattt atttgaggag
      301 ataatgatgc catcgaaagg caagaataac tggaaactcg atcaacaaag gaaaactgac
      361 aagaattcaa ataatcggaa gaagcctgga atgccagaag gctttcagtt taggttttcc
      421 ttagggtatg tgctcatagc acttttggct gtctctttct tcaattactt tctcttcaag
      481 agcgatacca ctcttgttcc ttattcggcc ttcaaggaaa aggttcgaag tggcgagatc
      541 cgtagagtgg aaattgatgt gaactattat accggttata cagagcccag tccttcggaa
      601 ccgtcttctc ctcttccacc atctgcaaaa ccaggtgttg tctataagac tgttccggtc
      661 agcgatcctg aattcacttc gctgcttgac gaaaaaggtg tagtatattc ggcgtctccg
      721 cgtggaggaa gcacgatact ctcgctcttg ctcaattgga tacttccatt tgcaataatg
      781 attttcttat ggcgcactat tatgagtagg tttatgggat ctaactcgaa tgtgctagcc
      841 tttggacaga ataaagccac agttgttgcg gaaggcgaca taaagacgag atttgcggat
      901 gtcgctggag tcgatgaagc taaggctgaa ttggtagaag ttgtcgactt tctcaaaaat
      961 ccacaaaaat atacagaaat cggcgggaag attcccaagg gtgtgctcct tgtagggcct
     1021 ccgggaacag gaaaaacctt gttagccagg gcagttgccg gcgaggccaa tgtgcctttc
     1081 ttcaagatga gtggtgctga ttttgttgaa atgttcgttg gagtcggtgc cgcccgagtc
     1141 cgcgatctct ttaaacaagc cagagagaag gctccatgta taatcttcat cgacgaaata
     1201 gacgcccttg gtaaatcgcg ggcaagcgga tttatagggg gcaacgatga gcgcgagcag
     1261 acacttaatc agctattagt cgagatggat ggttttgatt cgacaagtgg cctcatcatt
     1321 cttgcagcaa caaataggcc tgatgtcctg gatccagctc ttttaagagc cggaagattc
     1381 gatcgtcaag tattagtaga taagcccgac atgaaaggtc gggaggagat tcttaagatt
     1441 catacaaaga atatcaaaat ggatccttcg gtcgatctgt ccaaaatagc tcgcagcact
     1501 ccgggtttcg caggggccga tctcgcgaac gtcgtcaatg aggcggctct attagcggtt
     1561 cgtgccggcc gaaagaaagt caaagaggaa gatttccaaa acgctatcga gaaggttatt
     1621 gccggtctag aaaaaaagaa tcgtatgatt aatccaaagg aaaagcagat agtggcagtg
     1681 catgagacag gccatgccat aacggccgct ttgactccag gtgccgatcc agttaggaaa
     1741 atctccattg tgcctcgagg ctatggagca cttggttaca ccctccagat gcctctcgaa
     1801 gatcgttatc tgatcaccga agaagaatta ctgggtaaga tcgatgtgct tctcggcgga
     1861 cgcgctgcgg aagagctggt attccagtcc atttccaccg gagcttccaa tgatattgcc
     1921 aatgccacgg agatagccaa gagaatgatc accgactatg gcatgtccga caaattcaag
     1981 aatgtggcct tgacgcgaag atcgtcgggg ctaataagcc ctcaacaagc ttccgatccc
     2041 tttgcaacaa aagagtatag cgaggaaaca cagcgataca tcgatgagac tatagcttcg
     2101 atgataaacg agcgatatcg gcatgttgtc aatctgctta cagaaaaaag ggatttgttg
     2161 aatcgcatat cttctgtatt gctcgaaaaa gaagtcatcg aagagtccga attcgctgag
     2221 cttcttgaag gagctggcgt ttctatgcca gaggcaataa aagagcccgt ggctgcagcg
     2281 gagtctgttc ctctgcctgg atcaacagag aaggttttca tttctccgac tccacatgaa
     2341 taagattcca ggagatttgg ctcaattcta agtcgatgag tcttgctggt tctctattcg
     2401 gctctcttcc ctactctatt ctatgtccag ctctcatcat tatttctttt ctagatcttt
     2461 tcatggctcc gtttctattc tactcccagt tctcttccgg gctccttgat taagcgactc
     2521 aaaatatact ctaaccaatc ctgaaaattg gcatataata aaatatattg acatttttac
     2581 tcttattgtc atattgacaa tataatccca ttgtgattga ttaagggcat ctgtcgtagt
     2641 gaataaggag atgtcgctgt atgtccattg ttgtcagatc aatttcagcc tatataccgt
     2701 cccttcgcat cactaacgaa gagcttgcaa tgcgcatgga tacgacggac gagtggatac
     2761 gctctcatac cggtatcggt tctcggcata ttgcgcctga tgggcttcaa acaagcgata
     2821 tggcaacgga agccgcaaag agtgctttag ataaggcgaa tattagtgtg caggagttgg
     2881 attatatcat tcttgcaaca gcaacacccg attatcttgg ctttcctgct acggcctgta
     2941 tcgttcagga taagatcggt gcttatgggg tcgcttcatt cgatgtagcc gccggttgta
     3001 caggtttcat ctatgcgctc aacatagcat caagcatgct cgaagcatcg catggtagaa
     3061 atgctctggt tataggtgcc gaaggcttga gtcgatttgt caactgggaa gatcgctcga
     3121 cagcagtctt gtttggcgat ggggctggtg ctgcgatact ctcgcggatc gatgaatcct
     3181 cccgcggatg cctttctttt attctcgggg ccgaaggaag tggaacaaaa gatctatttc
     3241 ttgttcagcc cgaaagaact aaggcctttg agcgccagga acccatcata ccattcatct
     3301 atatggacgg gaaaaaggtc tatgattttg cggtcaaatc aataactgtc ctaatccaaa
     3361 ggcttcttca taaaaccgct tataaaatcg aagattttac atggattgtt ccacatcagg
     3421 caaacgcgcg catcgttcaa gccgctgcca aacgcttatc gattcctttg gataaattct
     3481 atattaatat cgaagaatat gctaacacct ctgcggcaag catacctatc gctctcgcag
     3541 aaatgtgcga aaagggactc ttgaaaaaaa atgatctcat aatgcttgtt ggctttggtg
     3601 cagggctcac ttatggagcg gcggttattc gatggtgagg ctcttgtctt gcggaatatg
     3661 gctatgatat cgcataatat tggcaaatcg ttacttgtat atgaagactt ttgcagcaat
     3721 atggcaatgc aaggagaagg caagaatgca aaatgaagta gcagtgacgg gtcttggaat
     3781 cataagccct atagggaatt cgattgacga gttttggaaa aactgtgtca acggagcaac
     3841 cggcattggt cctatcacac acttcgatgc atcgaggctg gaaagcagga tagccgctga
     3901 agtaaagaat tttgatccat ccgtatggat cgatcgaaaa gaagcacgga aaatggcgct
     3961 tttttctcag tatgcggtcg ccgctgcggc tcaggcttgg ctagatgccg ggctgcctga
     4021 ctttgtccag aatgagactt tggagaataa atccgatatc gaccccaatc gcattgcggt
     4081 catcttgggc aatgggatag gaggcctaga agtatttacg ggatctcatg ccaaaatgct
     4141 gcaagatggg ccggatcgga tacctccaat gaccattccc caaatgattg cgaatgaagc
     4201 tgccgcaaat atagccatgc attttggtgt tcatggtcct gttttgacac aggttacggc
     4261 ttgttcctca ggcaccgatg cgcttggcga ggcactggat ctcattcgtg ccggacgggc
     4321 cgatatcgta attgcaggag gaacagaagc agccatcact gaatttgcgg ttggctcctt
     4381 ttgcaggcta aaggcgctgt cgacttccta taacgagcga ccggaggtag catccaggcc
     4441 tttcgaccgc gatagggatg gttttgtcat aggagaagga gcaggtattt taattctcga
     4501 gagactcgat tacgccaagg cccgaaacgc gaaaatccga gcaatctttg cgggttatgg
     4561 cgctagttgc gatgcttatc atctaactgc tcctcagccg gaaggtgttt ttggagtaaa
     4621 agccatagaa catgccctaa gagatgctga tttgcaatca gcagatatcg aatactacaa
     4681 cgcacatggc acaggcaccg acctcaatga ccctatggaa accagaatgc taaagaaagc
     4741 ctttggttcc tctgtacaat cgctgaagat cagctcgaca aagagcatga cggggcattg
     4801 tatcgcggct gctggagcga tcgaagctat tgcatgtata aaagcgctag aaacaggcat
     4861 gcttcctcct acgattcact tagataatcc agacatagag cagggctgcg acctggacta
     4921 tattccaaat actgctcagc atcgcaagat taaaaccgcg atgtcggcta gccttggctt
     4981 tggcggacac aatggaatta tcatattcaa ggcagttgga gcataaaacc tgttgcagga
     5041 ttcaaatcag atggctaaga ttgcttagtt tagcaaagga atcagtttgc agaccagata
     5101 aggagagatt gaacggtgat gaatgttgaa tcttatcttc ctcatagacc tccatttctg
     5161 tttgtcgatg atgttcatat caaaggcgat actatactgg ctcaacatac attccgagac
     5221 gatgattggt tttttaaagg gcattttcct tcctttccta ttgtgcccgg agtcttgttg
     5281 gtagagagta tggcccaagc gggcggagta ggtgtaaaaa tgatgggcat ctatcccaag
     5341 agcctcttta tgtttgcaaa gataaaagaa gcgcgtttca gacagcccgt gcgccccggt
     5401 gatactctga tcatcaaaat aaagaatgtg cgtgcctctt ctgtctatct tcatcaaaaa
     5461 ggcataggta aggtaggcga taaggtagct gtggaagccg aatggataag catagcttca
     5521 ggagtaccgg aatgataata aagccaatga tccgaaacaa tatctgcctc aatgcccatc
     5581 ccgagggttg ccgtaaggaa gtgcgtctgc aggcagcgcg cgtaatgaat tttcgtgaaa
     5641 ggctctctaa aagtagcatc aatgactcaa gcgcaaaacc aaatcatgcc ttgataattg
     5701 gctgttctac aggatatgga cttgcatcga gactcgtcgc cgctttcgga tatggagctg
     5761 agacggtcgg tttttcttac gagaaagaac cttctgctac caaacctgca actccgggat
     5821 ggtatgcaaa taaggaattc gatagactgg ctcaagaaaa gggtcttttc gcagcatctt
     5881 tctcgcaaat agacgccttt tctcacacgg ccaaacagaa ggccatcgaa attgcgctgg
     5941 agaataaatt caaatacgat ttggtaatat actcgctagc aagctccgta cgggtcgatc
     6001 ctgatacagg ggtgctttac cgttcggtaa taaaaccaat aggaagacaa tacgctggcc
     6061 gcacaataga tatatttacc gaaaaattct cggaagcgtc ggttcagcct gcgacagaag
     6121 aagagatcac tcaaaccata aaggtcatgg gcggtgaaga ttggcagcta tggatcgatg
     6181 ctctcaagaa aaaggatttg ctcgcgccgg aagccttgac cctagcatat tcctacatag
     6241 gccctccttt ttcttggccc atatatcgcg atggtacaat cggtcaagcc aaactccatc
     6301 tggaagaaac tgcaaaactg ctagaaaaga actatggcaa ggataacggg cttcgatcct
     6361 ttgtttccat aaataaggca gtcgtaacga gagcaagcgc cgtgattccg atcattccgc
     6421 tatatatctc aacccttttc aaggtaatga aggagcgcaa tatacatgaa gactgcctgg
     6481 accagatgat gcgccttttc tctgaaagac tataccaaaa gagagggaca ggcatcccta
     6541 cggatgaaga aggcagaatt cgtatcgatg atctcgagat gggtccttcg gttcaaaatg
     6601 aaatcagttc tcgcctggct agaatcgatg aatcgaacct ccatgaactg gccgatgtca
     6661 aaggcttcag aagcgatttt ctaagggcac atggcttcga tgttcccggt gtcgactacg
     6721 agcgcgatat cgtctccttc gaatgaatat cttaatactg gattcatctt ccgaaactca
     6781 gtttctctct ttaaaagacc gcagaactca acatattatc gaagtcctta aaaaagaggt
     6841 cggtggcaga atgcgcgctg gcagtcttaa tggattggtt ggctgggcaa tcttagaatc
     6901 tatcggcaat acagagatag agcttcgttt tgtggccgaa ggaaccgctc ctccgctaag
     6961 gcctcttcgt atactcatag gaacagtaag accaattcag gctgcccgta tagtaaaaga
     7021 gctagccatt ctaggtgtcg agagcattgt attcttgccg accgaactgg gggaaaaatc
     7081 atacactcaa tcgcacttct accaacaaaa ggaatatttg gcatacgcgc gagaaggagc
     7141 ggaacaatca ggaaatccaa ggctacctga aattgggctc gcctggagcc tggatagagc
     7201 cctgattaag gcagagcgct ttgagagcat ggatcacacc aatacgataa atcccgaatc
     7261 agccggggaa gcgaaaatta acaaatgtac taagttgctc tgccaccctg acccatatgc
     7321 taaacttctt agcgagataa ttctttcgga attgccggtt atactggcca taggaacaga
     7381 gcgcggatgg acaaagcacg agatagatat ttttctcaac gccggctatc tgccttgctc
     7441 tctcggagat cggatattga aaaccgagac cgctgccttt gctgcggtct caatcttgct
     7501 ctctcgtttg ggatatatgt agagtcccct attacaggct ttctcttata atcttgcctt
     7561 caatatagca gaaagagcct ccacatcttc ggcaaagcgc ctgcaagcgg cttctactgg
     7621 ctcaggagtc gacatgtcga caccggcaag ctttaaggat tctatcggaa atcgactacc
     7681 tccggatttc agaaaatcga gataatcttc gcgctccttt tttccgcctt tcaaaactct
     7741 ctcggagaga gcaatacctg ccgacaagcc tgttgcatac ttgtaaacat agaatgcatt
     7801 atagaaatga ggtatgcgaa gtccttcaag atcggagata ttttctaaga tcatttctgg
     7861 cccgaaatat ctctcgagga ggtttcgata ctcttttcta agcacatcga ccgtaattgc
     7921 ttctcccgac tccgcaatct catgaactct ctgttcaaat tcagcaaaca tagtctgtcg
     7981 aaaaagtgtt cccagggtat tgtcgattcg atttgcaagc agataagcct tttctgcatc
     8041 atctttactg ttctcataga gatacgagaa aaccaattgc tcattgaaag tactggcaac
     8101 ctcggcttca aaaatcgtat atccataaca catgaaagga ttattgcgag cggaatacca
     8161 ggaatgcatc gaatggccac cctcatgggc gattgtaaat agatcatcga gtacatcttc
     8221 tttatagttc ataaggatgt aaggatctcc tagaaaacta cccgcggaaa aagcccctga
     8281 acgcttgcct ttattctcat agcgatccac ccagcctccc aatagccctc ttcgcagggt
     8341 actcacatac tcctccccca aaggcctaag tgcttcgatt actatgttca ccgcctgttc
     8401 atagcttcgc tcgattttga tttcctggac aagaggatta tagacatcat agtgccttag
     8461 agaatccaaa ccgagtagtt cctttcttag tttgtaatag tgatgcaaag caggtatgtt
     8521 cttatttacg gtagagacca ggttatcata aacagcctct ggaacattat ctgggaagag
     8581 tgccatagcc ctggcgcttg gatattttct cactttagct ctatatttat ccaattttac
     8641 agatccggca tacaacgccg caagcgtatg ccgatgcgct tcaaaggagg catagaattg
     8701 tagataggcc tttcttctta ctgatctgtc tggcaaacgc ataaagtatg caaaagtgga
     8761 ttgtgtaagc ggctttggac cttccggtat ctcgattgtg ccaaaatcaa agtcaacgtt
     8821 ggtcagaacg ctgaaggcat cctttggagt ctgggctgcc tcgctctgaa gagccaaaag
     8881 tcgctcctca gcttcactca gcacatgcgg tttgaagcga cgcaatttcg aaagaaaaac
     8941 cctataattc tcaaaagcag agtccttaag acagctgtcg aaaaaatcgt catctagcat
     9001 ctggatggca ggactgagcc aagaccaggc ggcttgaccc tgggtagata gatttacata
     9061 gcgctcaagc atgcctctag ctatgctatt tccttcatct tcggttactc tgaggtctgc
     9121 ataatatcca agcctttcct ctagaattag atattcttca aaggccttga gtatttcgag
     9181 aaaggccttc ctatctgcac caagagagag cctgctcttt tccgcatcaa gaatcattgc
     9241 attgaaggtc acaagttctc tttcccattc ggaatcgtca gagaagagac ttgccagatt
     9301 ccatctatgc tctgcaggaa catttgaacg ctctggaatt gtctttgcca ttatttctcc
     9361 cttttctaaa gcaatccaat attatttata tatcatgccg attcacgcac aataagctga
     9421 tatggcaatt ttatgtgttg gccagaagat ccatttagca tatttattac catgcgtgcg
     9481 gcctcggctc ccttatctcg gtcaggctgg tggatagtgg tcagacgagg agaaaccagc
     9541 acactctccg gaatatcatc aaatcccgct acagcaacat cttccggtat ctccagacct
     9601 agttctcttg cagcaatcat agctcctatc gcggtaatat ccgacattgc aacaatcgca
     9661 gttggcctct gatcaagcga aagtatatgg taagcgcttt catagccgct ctgtatcgag
     9721 cctttacagt tttggagaaa tacccgagta tccgtaatcg acaatccagc atcttttaat
     9781 accgcttgaa atccttcaag gcgtctctga acaacaatcg aagaaatctc ggatccgctc
     9841 ggcttcgtat cgggttccag tgccagtatt gcgagtcgct gatggcctct cttgagcaca
     9901 tgccgcatca aatctgctgc agcattcttc tgatcgattc caatgtttat tattgaagct
     9961 gattcttcgc catcgatggt cacaaaggga atgtgattct gcttaagcag gcttacgacc
    10021 tcatgacctg gcccgacgcc aatagtgatg agcccatcga cgtaagattt tcttgccgct
    10081 tcgattattc tacccctgat cagaggcaga atggcgagag agaattccct tcgctcgcat
    10141 tcctcgccta taccgctgat aacttgagtc atgtgcggat ttgcaaaaat atctccaatc
    10201 ttttgaggca ggagaagacc aattgcaccg agtcgctttg tcgtcaggct gcgtgcaaag
    10261 gggttgggaa cataaccgag ttcatcgatt atcgccatta ttcttttata agtctctttt
    10321 gataaccgat cagggtggtt tagcgcaaaa gaaacagtgg tcttcgatac tcccgcacgt
    10381 tgagctatat ccttgattgt cactttagac atgaggtgaa ttctacacaa aaacaataag
    10441 ctcgtcaaag tactcgtaat aacctataat agactagaaa tgaacactat gactttgact
    10501 atcgatggtt acaaagagat ttccgttcca gtgggatcga gtgcagagga aatctttgca
    10561 caggctggga tggaacgaga atggggaact tgtgaacatc cttttcctcc tctagccgct
    10621 cttgtcaaca acgaactcac ttctctctcc gcaccgatcg atgcagactg cagaattgct
    10681 ccaatatatc ccgattctcc tatgggcgcc gaggtatatc gccattcctt gtgttttctc
    10741 ctagctatgg ctgcaaaaaa tatcgcacca aagaggcgac tcattgtgag catggcaata
    10801 gggaatggat attatcacta cttcgatgat gaggaacccg tctcaccaag ccttcttgag
    10861 tccctgtgct tgtatatgcg cgaaatagtg gcccaaaatc tgcccatccg gattgtcctg
    10921 caatcctgga atgtcgtctt tgaatatttt aagcattcaa atcaagcaga gacttttgct
    10981 ttgatggaat atatgaatga tcccttcatc atgcttaatg aatgccagga ttatagggac
    11041 ttgcatattt cacctcttgt gccgcggacc ggacttctca gtgtatggga gctgattccg
    11101 tatcgtcgcg ggatattgct ccgttttcct catacaaaaa agccttacga aatggaccct
    11161 ttcgtagaca tacctgtgct ttatgatata gctgaagaat atgaacagaa agccaaagtg
    11221 ctaaatgccg gatctatcgg tgctctaaat aagataaatc aattggggga gattcaggat
    11281 tttgttctga tggccgaagc tctccaaaat aaaaaactcg cctcaattgc agacattatc
    11341 gccgaaaaat cagacaagac aagggtcatt ctgattgcgg ggccttcgag ttcgggcaaa
    11401 accacaaccg caaaaaaact atcggtgcag ctgagggctc ttggtttcaa gcctatccat
    11461 atcgagctcg acaactattt tgttgatcga agtcgaacac ccctcgacaa ggatggcaag
    11521 ccggatttcg aatgcctgga ggctctcgat atagaatttc tcaatcgaca gctcttggac
    11581 ctattcgagg gtaaagagat cgaacttccg gtatttgatt tcaaaagcgg cataagaaag
    11641 ccttccggca ctttcatcag catcaaagag aatgaaatac tgattctgga aggtattcat
    11701 ggtctaaatg agcgccttac accacagata tccgcggata ataagctcaa aatctatgtc
    11761 tcagcactga cacaactaaa tgtggacgat cacaaccgcg tgcgaacaac agactatcgt
    11821 ctcttgcggc gtatggtaag agattacaat tttcgcggcc atgatgctcg ccaaacgctg
    11881 gctatgtggt cttcggtcca gaggggagaa agactatata tcttcccctt ccagggtgat
    11941 gcagatattg cattcaattc cgcgctcgac tatgagctcg gagtgctcaa ggtatttgtc
    12001 gagccgcttc tgcgaacagt aaagccccat cacgtcgaat atacggatgc ccgccggatt
    12061 caggcgttca tctcgcggat ttcgcctatc tcgcctcagt atgtgcccag cgattcaatt
    12121 ctgcgtgaat ttattggatc gagcgtattc aagtattagg gtgccaccgc tctcaattag
    12181 cctccgattg cggtctcttt gaaagaagta acctatggtt tctttcttac ctcggacaat
    12241 gcaaaatcga aggcttcctg cagcatctcc tctggtatag gcgcaatatg accctctttc
    12301 atcagatctt gcgtcaaaga gcgcgcagct aagatatcgg ccttggcctt ggagtagata
    12361 ttatcccagg tatcgggaag tctggccaca ccctcttcta ctgctgccat agccacatcg
    12421 gctgcctcgc gcgcaaagac ttcggtctcc tccattgtgg caattatgtt gtcggaactt
    12481 atgccgcgcg attttgcaaa atcggcaata gagtgggcgc atcgaatcgc catatggtcg
    12541 gttatctttc tggagcgaac aagcaaagct cctttcaaga gaccgggaaa gcagatggaa
    12601 ttattgacct gatttggaaa atcgcctcga cctgtggcaa caatgtaagc gccctcttcc
    12661 ttggctgcat aaggccagat ctcaggcacc ggattggcac aggcaaagac aatggatttt
    12721 ggagccattg accgaatcca ttcgcgtttg acagtatctg gtcccggcgt cgacagcgca
    12781 ataagcacgt cggcaccctt tagggcttct tcctccgttt cgatgcatcg gggattagtc
    12841 tgaaggcaga gctcccattt tcgatagtat ctcttgtcgg cctcgatatc tcttcgattc
    12901 ctatgtaagc cccccttgct atcgaaaatc accatgttct ctcccctacc gccgtcggca
    12961 aggattaatc tggcaattgt cgtattagaa gcacccgcac ccagcaatac aattttggct
    13021 tcatttagct tcttgccggc aagctcaagt gcatttagaa gaccggcaag agtcacacaa
    13081 gcggtgccct gcgcgtcatc gtgccatacg ggaatttcgc aggcttccct cagttcatcc
    13141 agcactttga agcaatttgg ctggctgata tcctcaaggt ttacggctcc taccgagggt
    13201 tccagcattt taacaaaatc gatgatctta tcgggattgg gttttccatc gccgtctctg
    13261 gaattgatgc aaagcgcaat tgcgtctaca ccacccaaat acttcatcag aaaagccttt
    13321 ccttccatta ctcccaggcc tcccggaggc gtacaatcgc catcacccaa tacacgtgta
    13381 gagtcagaca caacggccac aagattcccc cggttggaga gcgcaaagga ttgatcatta
    13441 tcatctcgaa tcgttgtcga tatctttgaa acgcctggcg tataccatac attgaaccag
    13501 ttgaatccat agagacctgc cttgggcacg acctggatct ttcccccgta aaaacgatga
    13561 gtcttatctg aaagcgtttt cagaaaaatc gtttgtgcac gagctcgctg atccggtgtc
    13621 aaatcctccg gaaaagcttc cgatagattg tctagactga gattgagctt catagtttcc
    13681 ttccttttgc tatttgaccc ctttagaaca atttaggtct ttcgtctatt gtcaattgtc
    13741 gccgaacaat tggtcggctg gatatggtct tatactatca tgcgctttga ggtggtgcaa
    13801 taaaaagaga aagcacaacc tattgcgcaa tcacccggac tgacatatct atcagtatat
    13861 atctgtcaag gccttgatgt attgaagacg atggtactcc tccggtctat cggcattgat
    13921 agtggcgagt ttcccctgaa ggctatccat gcatgaatag ccaagggaat ccataaggat
    13981 attcatgtct ttgagcatag tcccaatgat tccccagcct tgtttataga taacagaaca
    14041 tacttcgact gcacttgctc cagcaagcaa gcattttatg gctgcatctg cactgtgaat
    14101 cccggtggat gaaacgatct caagaccgac ccgcttgtat aggatcgaag tccatcgcag
    14161 actctcatgg tattcggttt cggaactaag cggttgtgct ccggagagtc tcattgtact
    14221 caaattgaag tcaaacctaa aaaaccggtt aaaaataacg gctgcatttg cgccctcttt
    14281 agaaagccca tggactagat acggtaagct tgaataatgg ttaccgagct ttaccataat
    14341 tgggagcttt gtgacagatc gtgcttcctt aactgtggag agcacccttt tctcgatttc
    14401 ttgtcctgag atctgtggag aaaatggcac ataggctata ttcagctcta gcgcatctgc
    14461 accggcttct tctatgcgtc cagcaaaatc tgcccatctg ccttggccaa cacaattgat
    14521 gcttgcaatt attgggactg tgtccacttt tctctttgat tcgcttataa gtttaagata
    14581 tttatctgtg ctatcttccc aagccgtacg agaaatgaaa gcctctgcat ctggatgact
    14641 atttagatcg agccctgcca tttcttgtcc aagcacagca agtatttgtt cctcaaagag
    14701 cgatttgagc acaattgccc caacgccaga atgcgctgcc ttctctacac catcaggcat
    14761 actggtaagt ccgcttgcac caatgattat gggattttgt agctgaagcc ccatcaggta
    14821 tgtccgtaag tccgccattg tcagctcctt gacctcttat tgtaccagaa aaatagcagg
    14881 gcaagtagaa caaaaattat aaatctagcg atttggtttg gattttttcc aaaacgcgtg
    14941 cagcgaatac ctcgagagga atcccgctct cttgatctcc tgttctcagt cggatcgata
    15001 ccgtgccact ctcttgctcg cgctggccaa ctatagccat atagggaatc ctctgattct
    15061 gggcatctcg aatcttagca ttcattcgtt cttccgagat caatgccttg gctctaattc
    15121 ccttctcttg caaaaacctg gctactgttt tggcgtatcc ttcgaaacca ggcgctaccg
    15181 gtattatcgc tacctgatcc ggcatgagcc atacggggaa ggctccggca taatgctcga
    15241 tgaaaacccc aaaaaaacgt tcaagagaac ctaatagggc gcggtgtacc atataaggac
    15301 gcttttgctt gccatccttg tcgataaaaa ccatatcaaa acgttcgggt tcattgaaat
    15361 caaactgaat cgtagtcatc tgccattctc gacctattgc atcctttact tttagatcta
    15421 tttttggtcc atagaatgcg cctccgcctt catcgacctc atagagcaat ccttctttat
    15481 cgattgcctt cttgagggac aaaagagcct gatcccagcg ctccggctcc cccactgaat
    15541 ccgaggggcg ggttgcaaga taagccttta tttccttaaa acccagtgtt ttccatattg
    15601 aaaggctaaa tctaagtacc tctaatattt catcctcgat ttgatccgga gtgcagatga
    15661 tgtgagcatc atcctgtgta aatccacgaa ccctcataag cccatgaagg acccctgaac
    15721 gctcatagcg atagacagta ccaagttcag cccagcgaag tggaagatcg cggtaagaat
    15781 gactggatgt tttatatatc ataatatgga atggacaatt cattggctta atgaagtact
    15841 cgtcttcatc tatcttcatt ggggcataca tgttcgcttt gtagaagccc aggtgaccgc
    15901 tggtctccca gagccagctt ttgccgatat gaggcgtaaa gagaatctca tatccgttct
    15961 tataatgttc atctcgccac cagttttcca gttcgacacg aatacgaccc cctttagggt
    16021 gccagtatat gagaccggga ccggcttcct catgggtaga gaagaggtcg agctctttgc
    16081 ctaatttgcg atgatctcgt cgctccgcct cctccagcat gtggagatat tcctctagtt
    16141 tggccttcga ttcaaaggcg taggcataga tcctcgtcaa cattggcttt gattcgtctc
    16201 cacgccaata ggcaccagca atagtcttga gtttaaaggc atctggcctt atctctcttg
    16261 tggatttcac atgtggtccc cggcagagat ctacaaaatc accctgcttg tatatcgaaa
    16321 ttgtgccttc ttccaagcct tcgatcaatt cgagtttgaa aggttgatcc ctgaagag
//
LOCUS       DAOMZL010000039        16220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_3235_length_16220_cov_14.344262, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000039 DAOMZL010000000
VERSION     DAOMZL010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 16220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16220
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3235_length_16220_cov_14.344262"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1281)
                     /locus_tag="PLQ30_04710"
     CDS             complement(130..1281)
                     /locus_tag="PLQ30_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01778.1"
                     /translation="MKRFVGLLCLIIIANILVSCQSSMEEGKIQQLFDLGYGKSDSSI
                     DISVQNKNSVDICLRKGIFHLLSQEEAKILRISSYGQTLAMWYDPKRGSAPLVLEDVQ
                     MSDFSSGKDLGRFAIQVSFEDPGHIASDSRQMLYVSDAEIVRRFDSDGRELPFLGQEG
                     IGGIRFPVITSLDVLSNDDIAVSCSSESGKAVYFYDRNGTFLHVLKWDGQHMPIPVSI
                     AENHPNTGGSSIIASLESFLPCFLNGRRVVMLKINYYYEISDPSSGVTISIEPVGGWI
                     IETDASNGALLNSFPLQSSLEDLGIDQQMIAAQSNGIFCVRWRDKGLSGEVFRFNQQG
                     KINGKLDFQVPDADASLIDMVVGDDGNLYMLTQLSTELKMYTWRILTLR"
     gene            1337..4108
                     /gene="secA"
                     /locus_tag="PLQ30_04715"
     CDS             1337..4108
                     /gene="secA"
                     /locus_tag="PLQ30_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPW01779.1"
                     /translation="MSRNTFLSALLGSKEEKDIKAVLPLLHKINELEPWALSLADSDF
                     PAMTEKFKGRLANGETLDDLLPEAFALVREAARRKLGERPFDVQIIGGIALHQGKIVE
                     MKTGEGKTLSSVTAAYLNALTGQGVHIITVNDYLAERDSQWMGQIYKFLGLTVGCILS
                     AMDNIARKKAYLSDITYGTNNEFGFDYLRDNMVWSLDQKVQRGHAYCIVDEIDSILID
                     EARTPLIISGPADDDTYKVNEVNRLASSLFEVKKNPETGDYPDEARGELLEGDFKIDE
                     KSKRVMFTSEGLNHIESLLQKRGLIKGSLFDPENFQFVHYFTQAVRAQHLFSKDVDYV
                     VQDNQVQIVDEFTGRILHGRRYSDGLHEAIEAKERIKIARRNRTLATITFQNYFRLYK
                     KLAGMTGTAETEAEEFNKIYNLDVMIIPTNRPVVRKDENDLVYLNEAEKYDAIIGAIR
                     EIHTKGQPILVGTVSIEKSEKLSALLIKSGVKHEILNAKNHAREALIIAEAGSKGAVT
                     IATNMAGRGTDIKLGGNPEFRARRKAGTEAPEEKYREILSREYETWTKDYEEVKSLGG
                     LCVIGTERHEARRIDNQLRGRSGRQGDPGRSAFFISLDDDLMRLFGGDNFKQLMGKAG
                     MKPGEPIYHPLLSKSIESAQKKVEERNFEIRKHLLEYDDVLNKQRNFIYEQRNSILAD
                     DNLIERVRDAAYEMLEEQNAILSQAFKDNANKAIQEFGSWLFDSFGIEMTTKELGALY
                     KSGGLVSEIKNNLDLDLDAKIQVAGAENLNIFIRFSYLQEIDEKWLNHLESMEALREA
                     VYLRSYAQRNPLLEYKIEGSDIFESLIFSIRHNIASKVFRVRIKESTPTVEATSPRST
                     SRIEAAQTSHEAVSSFVDQSKSVQKESAIALASTPATATVVRQGKKIGRNDPCPCGSG
                     KKYKYCHGR"
     gene            4169..5614
                     /locus_tag="PLQ30_04720"
     CDS             4169..5614
                     /locus_tag="PLQ30_04720"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HPW01780.1"
                     /translation="MSLNCEEIDLILNEAPLVGMKIQNVYQPTFDSLVLELFGKGILT
                     QYYFSIAHNACRLHPLSTPAPKNERPLRFMECLRSRIRGGTILHAAQISKDRIVKIDI
                     IRANEKGVAEQSYLYARLWSGAGNIILVSVEGIIIDALRRLPARNEVSGAAFVLPQQL
                     DSNKQPSKHYVVRDLEGKGTFWQKIEQFYAQNADNLSRSTLLTQAHDRYERKKISIET
                     KLNHLVSKQQEYEQAERFRQLGDILLAGYCPEIQGRKAFARVYDYYENKEILIEIDQN
                     LNPAKNAAIYYEKYRKAKSGLEEIRSEIQYWEKSLNHLDAWLNRLEKETNPLIIAKIL
                     RKGGAARSKEKPAFPCQYLDFRGWRILIGRSGKENEDILRHIVRGSDLWLHARDYAGS
                     YVFIKAKKNKSIPPEVLLAAAKLAIYYSKARKNGGGDVHTAFVKNLRKAKEGPIGRVI
                     PYFEKNLYITFTEAEIKEIFQEYQSKEENYV"
     gene            5611..6576
                     /locus_tag="PLQ30_04725"
     CDS             5611..6576
                     /locus_tag="PLQ30_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW01781.1"
                     /translation="MKRSKISLVATLLLAVSVFILYASPKDKIAVSVIRGPSGLSSTW
                     MMSELARTSPSEFEFISVAGADMVVAKLLNGEIDAGVLPVNVAAKLYNTGAPIRALAV
                     VGNGMVKFLTTDTKVQSLADCKGKTIYIAGQKATPDYIFQFLCAQKGLQIERDYTAIY
                     SLAYPEIAAGLAGGKISYAVLPEPFASQAILKNPAIRTPIDITKEWQAATDQPDYPMS
                     LFVARKEMIEAYPDKARILLDSYRASINKAIEDPQLIAKLAENLDLGVNAQTAIIAIP
                     KSNFVFVEAYQAIPSIEALLSVFLKFDPASVGGVLPSKSFYAQIK"
     gene            6560..7357
                     /locus_tag="PLQ30_04730"
     CDS             6560..7357
                     /locus_tag="PLQ30_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPW01782.1"
                     /translation="MRKSSEHRFMLLRWQLLGIISFILLWEIGALLVKSDLILPSPAR
                     VAKALYQLFGKNFFWLSIGGSFLRVFEAFILSGLIGLATGTMSGIKPQARAFLNPFIV
                     GIRATPVMALILVAMFWFPAWQVPVFSAILMAFPVMHTSTEAGVHAVDKKLLEMSTFF
                     NVPSSFIFWRLRLPSALPYILSGAKNALGLSWKVVVAGEVLSQPRMALGTGMQEARLS
                     IETATVFAWAIITIFLCGLSEYLFGLLADRLSARFQQSSQEEDQDLS"
     gene            7360..8094
                     /locus_tag="PLQ30_04735"
     CDS             7360..8094
                     /locus_tag="PLQ30_04735"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW01783.1"
                     /translation="MNIKIDNLRKSYGALNVLEDVTFSLEESRITSILGPSGCGKTTI
                     LNIMSGILQADSGSIIGIEGRKFSYCFQEPRLLGWLSAEDNLRFALSSLFQSSPRTND
                     IELRIERFLREAGLWEFRKFKPSQLSGGMQKRLALARAFAFPADMLLLDEAFSEVDLR
                     KKMELMEDFLHLWKDEEPTVVIVTHDIHDALYLADQVIVLSQRPAIVEGTILIDIPHE
                     KRTFTSSELYGYEKELYRLLGLDRTV"
     gene            complement(8071..9978)
                     /locus_tag="PLQ30_04740"
     CDS             complement(8071..9978)
                     /locus_tag="PLQ30_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW01784.1"
                     /translation="MPRVAILESDILIALDISKSIEQEGLAEVDIYEELEQAYQKLNN
                     LEHELFIIDIGDNQPEIIDAAVAIHRQTGLYCLVISDHGVTSIPARLREAQPLGILVK
                     PFSTRELIVNVETALYHSSMEKKLRDSERRYKDLFAYSLSARCVTDIEGRILERNRVF
                     ESSFSNPMKVENVRDILPNDIIWSEIIESLEENQILQREIQTNRISDSFEQRSLDLIC
                     SFSRFREEGSDAQILCEFLDVTESRRLREELFQSQKREEIGRLTSGVAHDLNNFLTSI
                     MGFLEMIKLEIPDDSQINEDIQGIERVVNRASGLTRQLLGFSRHKPYSPAPIDLKEVM
                     GDTYKILKRLIPENISLTLSLPKESLVVNVDISHLEQILLNLVVNARDALEKTENPHI
                     SIQLSQFGQNALYPKEIMMQPADYALIVVKDNGAGIDNENIKRVFEPFFTTKKQNQGT
                     GLGLSIVKSLVEMNEGYITLESEKGRGTTIKIWFPILLETKTTTKLTAQEEYSLLSII
                     NPPNNILKGKHILVVDDDESILEACTRILESAGAIVGACINAGEAILQAERTNFDLLL
                     TDIVLPGVYGNELWDRMKNNGQARACVFMTGYDSYQNEQFAGAALLHKPFDARSLLEY
                     CNNALNSSIEA"
     gene            complement(10035..11960)
                     /locus_tag="PLQ30_04745"
     CDS             complement(10035..11960)
                     /locus_tag="PLQ30_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPW01785.1"
                     /translation="MKTRQAAILVLFLSLSIAAILSNCSSLPENEPNANSTGDSIISH
                     LDLLVDTDPEQCIAVISNLFEQQREKNQSFDNSIIGEKGIVLFRKAVENIGFLFKKAI
                     ASQDILTARRLSFSADALVQYVSFYDSLGEIVSEELSMIASPAMRLQLALWEAENKFK
                     EYGYVIGKATLSKAHLGEQEEYSFNKIEDFSIENLKSLIETRDPKEYFITWAERAKEN
                     NDVNAEAWFESLSSGQMSYSPSILVSDQDWLADAVSSVVTVYVDRGFKVEGGYSVPDR
                     VLGTAFQISEGLYLTNYHVVQSEIDPTYKGYSRISIRPSDNPQIRVPSKVLGWDEELD
                     LALIQSAEHARSTVMLPPPGMRFHAGDRVFAVGSPIGLENSITAGVVSSLSRKIISFG
                     EAAQIDVPVNQGNSGSPLFSENGVLVGMVFAGLPSFQNINFALPVQWISLSLPDLFER
                     KEVIHARLGLMMGKGSGQSPLVLADLDPSLSAFIAGDTLTSINGKLTKDIASIQFQLA
                     SVPKGALCFLEAQRDFKPVRRLRQISHTDGASLIPAWDRIHKNSILEGLFGARLTQLK
                     ESTKVGGLYSIQWVCPAEPADEIGLSENDVITVYKFRLDEKNKVFILEFSAKSRLSGY
                     FERTIHLEFSTESATII"
     gene            complement(11969..13852)
                     /gene="ftsH"
                     /locus_tag="PLQ30_04750"
     CDS             complement(11969..13852)
                     /gene="ftsH"
                     /locus_tag="PLQ30_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPW01786.1"
                     /translation="MPDQNDNRDGNKPDLSIPPKGNRAALVLFLSIIVLLVVFLFFGQ
                     DESSREISYSSFLSYLELGEVKSIKIMDQRDIEGVLKGKSGAEMPFTTRIPYFDAELM
                     AKLEEKGVSVTGAISGTSPFQILFGLTPWIFGFIMIWIMMRQMQGNNKAFSFGKSRAK
                     MYSNSNKKITFEDVAGQKEAKYELMEVVDFLKNPNKFLKMGAKIPKGVLLVGMPGTGK
                     TLIAKATAGEANVPFFHMSGSDFVEMFVGVGASRVRDLFEQGRKHAPCILFIDELDAV
                     GRTRGSGLGGGHDEREQTLNQMLVEMDGFDTKDGVIVLAATNRPDVLDPALLRPGRFD
                     RQVVVAMPDVNERTEILKIHMRQIPTSGDVDVEKLSRATPGTSGADLANLVNEAALFA
                     IRKNKELVEMDDFEDARDKILMGVARKSLVIAEEEKRATAIHESGHALLHYFLAHADS
                     LHKVTIVPRGRALGLALSLPDKDTYSRTYGWLYDRIVISYGGYAAEKIVYGETTTGAA
                     QDIKQATEIAQKMVREWGMAEEIGPISLGEEEEPIFLGREIAQHKDYSEATAQRIDAA
                     IQGILDSALDKAINILKREKNRLVRLADKLMQVETLEDKEVRELLGLPEGKILLEEGI
                     APC"
     gene            complement(13857..15329)
                     /gene="tilS"
                     /locus_tag="PLQ30_04755"
     CDS             complement(13857..15329)
                     /gene="tilS"
                     /locus_tag="PLQ30_04755"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPW01787.1"
                     /translation="MKTLEYKVKKFLLDMHVDFHKPLAIAFSGGLDSSVLLYILVRIF
                     DHDNIRAIHVCHNLRSKEELERERIFLQKTCKAYSIPITIVNIRQGAISEYAHRKNCG
                     IEAAARHYRYHALVRTAQRFGISTIVTAHQADDQAETFLLRLVRGGRLETLGGISASR
                     LVDSTKGINVIRPLLSFRRHELEAFARENNLEWSEDSTNRELSFLRNRIRHVLIPLLD
                     KEFPSWRSSIESYRWQAQELNTLITSIACQSMPKVMRGLGKDAILDQALFRKEEALVR
                     RRILRLFFMHLGLKDAVSQGALESLERAILQGAPRVEAARCQFDLSNGVVKYIGKANS
                     SSGFLDRSISQLKGLFRFDEFFLRVGEPGQYECGPLKMVVSQSVQPPSLDNASIDKEQ
                     FLRLSIPLSFPFIFRNRKEGENIGDGDRSKDLGSTSKNQKISADISHIVAILEDEYGI
                     AAILTMTFDGRFSIKSDYSSRLLCEKRKILYIFLSRKGDF"
     gene            complement(15479..16135)
                     /locus_tag="PLQ30_04760"
     CDS             complement(15479..16135)
                     /locus_tag="PLQ30_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPW01788.1"
                     /translation="MEHIALRVSPRGELTKGELRQERKKGRLPAQIYGKGIEPISVFL
                     EGKEFANVAKRVTESRIIDLDLEGKKFATLLKEIQKETISGNVIHIDFNLVRHDRNIH
                     VRVPLHLNGVAKGVKDGGILEHSIHEIEVECDSDHLPEKIEVDITDLEANHALHLKEV
                     ALPEGVKLVTNPDTVFAIIKFAHGEPEKALEPEAEEAGEAVASSEQESAETTSEAVHK
                     "
BASE COUNT         4612 a         3671 c         3385 g         4552 t
ORIGIN      
        1 ttctaaattc atagcgaaat gactatagcg gaaatgctat agaatgtcat cataaatgtt
       61 ccgaatcttt tgaatattgc tttttcgcta tacctaagtc atcaaaatca agcgctccaa
      121 tgacttgtct cacctcaagg tgagtattct ccatgtatac atcttcagtt cggtagatag
      181 ctgagtaagc atatataaat ttccatcatc accaactacc atatctataa gggaagcatc
      241 ggcatcaggt acttgaaagt ccaatttgcc atttattttc ccctgttgat taaagcggaa
      301 aacctctcca gataatcctt tatctctcca acgaacacaa aaaatgccat tcgattgggc
      361 agcgatcatc tgctgatcga tccccaagtc ttcaagactg ctctgcaatg gaaaagaatt
      421 tagaagcgct ccgtttgatg catctgtctc aataatccaa ccaccaacag gctcgataga
      481 aatcgtcact ccagatgaag gatcgcttat ctcgtaatag tagttgatct tgagcattac
      541 cactcttctt ccattcaaga agcaaggcag aaatgattca agcgaagcaa tgatgcttga
      601 accaccagta ttcggatgat tctcagctat tgaaacaggg attggcatgt gttgcccatc
      661 ccattttagc acatgcaaaa aggtgccatt tcgatcatag aaatagactg ccttccccga
      721 ctcggaagag caggaaactg caatatcatc attgctaaga acatctagcg aagtaattac
      781 cgggaatcta attcctccga tgccctcttg tccaagaaaa gggagctctc taccgtcgga
      841 atcaaatctc cttacaattt cggcatcaga aacatagagc atttgtcttg aatcgctagc
      901 aatatgacca ggatcttcaa atgaaacctg aatggcaaaa cgacccaggt cttttcctga
      961 agaaaagtca ctcatctgga catcctcaag gacaaggggt gcacttcctc tttttggatc
     1021 ataccacatt gcaagggtct ggccataaga agaaatacga agtatttttg cttcctcttg
     1081 gctcagtaga tgaaatattc ctttcctgag gcaaatatcg actgagttct tattctgtac
     1141 agaaatatcg atcgaagaat cggatttacc atagccaagg tcgaaaagtt gctgtatctt
     1201 tccttcttcc atagaggatt ggcagctcac aagaatatta gcaatgatta tcaagcatag
     1261 cagacctaca aatctcttca ttgcgagaat tataccattc cttgaccctt tttgagaaca
     1321 aaagtattct taacacatgt ctagaaacac atttctttca gccctcctgg gctcgaaaga
     1381 agagaaagat atcaaagctg ttttgccact tctccataag ataaacgaac tcgagccctg
     1441 ggctctgtct cttgctgata gcgactttcc tgccatgaca gagaaattca aaggccgcct
     1501 cgccaatgga gagaccctcg atgatctttt gcctgaggct ttcgctcttg tgagggaagc
     1561 ggcacgaaga aaactgggtg agcgtccatt cgatgttcag ataatcggag gaattgctct
     1621 gcatcaggga aaaatcgtag agatgaagac aggagaaggc aagactctgt cttcggtaac
     1681 cgctgcttac ttaaatgcac taactggaca aggtgtccac atcatcacgg taaacgacta
     1741 tttagccgag cgcgatagtc aatggatggg gcaaatctat aagtttcttg gacttactgt
     1801 tggatgcatc ctctcggcca tggacaatat agcaagaaag aaagcctatc tcagcgatat
     1861 cacctatgga actaacaacg aattcggttt cgattatctg cgcgataata tggtttggtc
     1921 tttggatcaa aaggtccaga gagggcatgc ttactgcatc gtcgatgaaa tcgattctat
     1981 ccttatcgat gaggcgagga caccgcttat aatctcaggc cctgcggacg acgacactta
     2041 caaggtaaat gaggtcaatc gcctggcttc gtcccttttc gaagtcaaaa agaatcctga
     2101 gacaggcgat tatcccgacg aagccagggg cgaacttctt gaaggtgatt ttaagataga
     2161 cgaaaaatcc aaacgagtca tgtttactag cgaaggattg aaccacatcg agtcattgct
     2221 acagaagaga ggattgataa agggaagtct ctttgaccca gaaaacttcc aatttgtcca
     2281 ttattttacg caagcggttc gagctcagca tctcttctct aaagatgtag attacgttgt
     2341 acaggacaat caagttcaaa tcgtcgatga gtttacaggt cgaattcttc atggcagacg
     2401 ctactcagat ggtcttcatg aggctatcga ggcaaaggaa agaatcaaga ttgccaggcg
     2461 caatcgaacc cttgctacaa taacctttca gaattacttt aggttatata aaaaactagc
     2521 cggaatgact ggaaccgctg aaacagaggc cgaagagttt aataaaatat acaacctcga
     2581 cgtaatgatc attcccacaa ataggcctgt tgttcgaaaa gatgagaacg accttgtata
     2641 tctcaatgaa gcagaaaagt atgatgccat aatcggcgcg ataagggaaa tacacacaaa
     2701 gggacaacct attctagtag gcacggtttc catagaaaaa tccgaaaagc tctctgcttt
     2761 gctcattaaa agtggcgtaa aacatgaaat attgaacgcc aagaaccatg cacgcgaagc
     2821 tctgataatt gcagaagcgg gttcgaaggg agcggttacc attgcgacaa atatggcagg
     2881 acgcggaaca gacatcaagc tcggaggaaa tccggagttt cgtgcaaggc gcaaggccgg
     2941 taccgaagca cctgaagaaa aatacagaga gatcttatct cgcgaatacg agacatggac
     3001 taaagattat gaagaagtca agagccttgg gggtctttgc gtcataggaa ccgagcgtca
     3061 tgaagcaagg cgcatcgaca atcagctgcg aggccgaagt gggcgtcagg gagatcctgg
     3121 acggtcggca ttttttattt cgctagatga tgatctgatg cgcctcttcg gaggagacaa
     3181 tttcaaacaa ctcatgggca aggctggcat gaagccagga gagcctatct atcatcctct
     3241 gctcagcaaa tcgatcgaaa gcgcacagaa aaaggtcgaa gaaaggaact ttgagatacg
     3301 aaaacacctc cttgaatacg atgatgttct aaataagcag cgcaacttca tttatgaaca
     3361 aagaaattca atcctagccg atgacaatct tatagagagg gtgcgggatg cagcctacga
     3421 aatgctggag gaacagaatg ctattctcag ccaggcattc aaagataatg caaataaggc
     3481 aatccaagaa ttcggctcgt ggctctttga tagctttggc atagaaatga caacaaaaga
     3541 actaggtgcg ctgtacaaat caggtgggct tgtttctgag atcaagaata atcttgatct
     3601 tgatctcgat gctaaaatac aagtggcagg tgcggagaac ctcaatattt tcattagatt
     3661 ttcttatttg caggaaatcg atgaaaaatg gcttaatcac ctggaatcga tggaagctct
     3721 tagagaagct gtgtatttgc gaagctatgc tcaaagaaat cctttgctcg aatataaaat
     3781 cgaaggatca gatattttcg aatccttgat cttttctatt cggcataata tcgcctcaaa
     3841 ggtctttaga gtaagaatca aagaaagtac cccaacggtc gaagcaacaa gtccacgatc
     3901 tacatcgagg atagaagcgg cacagacaag tcatgaagca gtaagttcct ttgtcgacca
     3961 aagcaaatca gtgcagaaag agtctgcgat agctttggca tccactccag ctacagccac
     4021 ggtggtcaga caaggaaaga aaatcggccg taatgatcca tgcccgtgcg gttcgggcaa
     4081 gaaatacaag tattgtcatg gccgatagaa ccgcttgata tcggaaatga tgatattgga
     4141 cgcttcctag aatggctgga gtttttctat gtcactaaac tgcgaagaaa tagatctcat
     4201 tctaaacgaa gctccacttg tcggaatgaa gattcaaaat gtctatcaac ctacttttga
     4261 ttctctcgta ttagaactat ttggcaaggg aatattaacc cagtattatt ttagcatcgc
     4321 ccataatgcc tgcagactcc atcctttaag tacccctgct cccaaaaacg aacgaccctt
     4381 gcgctttatg gaatgcctac gctcaagaat cagaggaggc actatattac acgcagcaca
     4441 gattagcaag gatcgcatag taaaaataga tattatcaga gcaaacgaaa agggggttgc
     4501 ggaacaatcc tatttatatg caagattatg gagcggagcg ggaaatatca tccttgtctc
     4561 tgttgaaggt atcataattg acgccctgcg acgcctaccg gctcgcaatg aagtatccgg
     4621 ggcagcattt gtgttgcctc agcagcttga ttccaataaa caaccttcca agcattacgt
     4681 ggttcgcgat ctggaaggca aaggcacatt ttggcaaaaa atagaacaat tttatgctca
     4741 aaatgcggat aatctctcta gatctactct gctcactcaa gcgcacgaca gatatgagag
     4801 aaaaaagatc tcaatagaga ctaaattgaa tcaccttgtt tccaaacaac aagaatatga
     4861 acaagccgag cgattccgcc aactgggtga tattctgctg gctggatatt gccctgagat
     4921 acaaggaaga aaagcatttg cgcgtgtcta cgattattac gagaataagg agattcttat
     4981 cgagatagat caaaatctca atcctgctaa gaatgctgcc atatattacg aaaaataccg
     5041 aaaagcgaaa tctggcctcg aagagattcg ttccgaaatt caatattggg aaaaaagcct
     5101 taatcatttg gacgcttggc tcaatagact tgagaaagag actaatcctt tgataatagc
     5161 caagattcta cgaaaaggtg gagctgctcg ctcaaaagag aagcctgctt ttccttgtca
     5221 atatctagat tttaggggtt ggagaatctt gatcggtcgt tctggcaaag aaaacgaaga
     5281 tatcctgcgt catattgtac gaggttcaga tttatggctg catgcacggg attatgctgg
     5341 ctcctatgta ttcatcaaag ccaagaaaaa caaatcgatt cccccagaag tacttctagc
     5401 tgcagcaaag ctcgcaatat actactcaaa agcccgcaaa aatggtggtg gcgatgttca
     5461 tacggccttt gtcaagaatc tgcggaaggc caaagagggg cctataggcc gggttattcc
     5521 ctatttcgag aaaaatttat atattacttt cacggaagct gagataaagg agatttttca
     5581 agaatatcaa tctaaagagg agaactatgt atgaagcgat ctaagatttc tctagttgcc
     5641 acattgttgc tggcggttag tgtttttatt ttatatgcat cgcctaaaga taagattgcg
     5701 gtgtcggtaa ttagaggtcc ttctgggtta agcagcacat ggatgatgtc tgagcttgcc
     5761 aggaccagcc cgagcgaatt cgaattcata tcggttgcgg gcgctgacat ggtagtcgca
     5821 aaacttctca atggagaaat agatgctggc gttttgcctg taaatgtggc cgctaagcta
     5881 tacaatacag gtgcgcccat aagagctttg gcggtagtgg gcaatggtat ggtaaaattc
     5941 ttgacaacag acaccaaggt gcagagtctt gcagactgca aaggcaagac aatctacatt
     6001 gcaggacaaa aagcgacccc tgattatatc tttcaattcc tatgcgcgca aaaaggcctt
     6061 caaatagaaa gagattacac agcaatctat agtctggcat atcctgagat agcagcaggg
     6121 cttgcaggcg gaaagatatc ttatgctgtg ttgccagaac cttttgcatc tcaagcgatt
     6181 ctcaaaaatc ctgctataag aacgcccata gacatcacaa aggaatggca ggcagcgaca
     6241 gatcagcctg attacccaat gtctctattt gtcgcgcgaa aagaaatgat tgaagcatat
     6301 ccagataagg caagaatctt gcttgattcg tatcgcgcct ctatcaataa ggctattgag
     6361 gatccgcaat tgatagcaaa acttgcagag aatctcgatc tgggcgtcaa tgctcaaaca
     6421 gcaattatcg ccataccaaa atccaatttt gtatttgttg aggcttatca ggcaatccca
     6481 tccatcgaag ctctgctctc ggtatttttg aaattcgatc ctgcaagcgt tggtggtgtg
     6541 ttgccaagca aatcattcta tgcgcaaatc aagtgaacac cgttttatgc tgcttcgatg
     6601 gcagctatta gggattattt catttatctt attatgggag ataggagctc tccttgtaaa
     6661 aagcgatcta attctgccaa gccctgctcg agttgccaaa gctctttatc agctttttgg
     6721 aaagaatttt ttttggcttt caattggagg ctctttcctt agggttttcg aagcgttcat
     6781 tcttagcggc cttataggcc ttgctactgg cactatgagc ggcattaagc ctcaggcgcg
     6841 ggctttcttg aatccattta ttgtgggaat tcgggcaaca ccagtcatgg cgctgatatt
     6901 agtggctatg ttttggtttc cggcctggca ggtacctgtt ttcagtgcaa ttctaatggc
     6961 gtttcctgta atgcatactt caacggaagc aggcgtgcat gcggtagata agaagttact
     7021 tgagatgtcg acctttttta atgttccttc atcatttatt ttctggcggc ttcgtttgcc
     7081 atccgcactt ccatatatcc tatctggagc aaaaaatgcc ctggggctct catggaaagt
     7141 ggtagttgct ggcgaggtct tgagtcaacc gcgcatggcc ttgggtaccg gtatgcagga
     7201 ggccaggctc tccattgaaa cagccacggt ttttgcttgg gcaataatca cgatctttct
     7261 ttgcggtcta agcgagtatc tattcggact tctcgcagat agattaagcg cgcgatttca
     7321 acaatcttct caagaagaag accaggatct gagctgaaaa tgaatatcaa gatagataat
     7381 ctgagaaaaa gctatggagc tctcaatgtg ctcgaggatg taactttttc tctcgaagaa
     7441 tctcgtataa cctcaatttt agggcccagc ggctgtggca agacgactat cctcaatata
     7501 atgtctggaa tcttacaagc cgatagcggt tctataattg gcattgaggg ccgcaaattt
     7561 tcctattgtt ttcaagagcc gagacttctc ggttggctca gcgcagaaga taacctgcga
     7621 tttgccttga gctctctatt tcaatcctct cctagaacaa acgacatcga attgagaatc
     7681 gaacgctttt tacgagaagc tggactttgg gaattcagga aatttaagcc tagccagtta
     7741 tcagggggaa tgcaaaagag gcttgctttg gcaagggcat ttgcttttcc agccgatatg
     7801 cttcttctgg atgaggcctt ttcagaagtc gacttaagaa aaaagatgga gcttatggag
     7861 gattttcttc atttatggaa ggatgaagag ccgactgtcg taattgtcac acatgacatc
     7921 cacgatgccc tatatctagc tgaccaggtg atagtcttga gtcagcgccc agccatagta
     7981 gaggggacta tattgattga tatacctcat gaaaaaagga cttttacttc ttcagaactc
     8041 tatggatatg aaaaggaact atatcgcctc ttaggcctcg atcgaactgt ttagagcatt
     8101 attgcaatac tctagaagag aacgggcatc aaatggcttg tgcagcaaag cggctcctgc
     8161 aaattgctca ttttgataag agtcataccc cgtcatgaag acacatgctc tggcctgacc
     8221 attgttcttc atcctatccc ataattcgtt tccatatacg cccggcagta ctatatctgt
     8281 gaggagaagg tcgaagttcg ttctctcagc ttgaagaata gcctctccgg cattgataca
     8341 agcaccaaca atagcacctg cactctcaag gattcttgtg cacgcctcca atattgactc
     8401 atcatcgtca acaacaagaa tatgctttcc tttcaatatg ttattaggtg gattgattat
     8461 ggagagcaaa gagtattctt cctgggctgt gagcttggtc gtggttttag tctctaaaag
     8521 aattgggaac cagattttga ttgtggttcc tcttcccttt tcgctttcaa gagtaatgta
     8581 gccctcattc atttctacaa gagatttaac gatagataat cccaaacctg ttccttgatt
     8641 ttgcttttta gtagtaaaaa aaggctcaaa tacccttttt atattctcat tatcaatgcc
     8701 agctccgttg tctttaacta ctattagagc atagtcggct ggctgcatca tgatttcttt
     8761 tggataaagt gcgttttgcc caaattggct aagctggatc gatatatgag gattctcggt
     8821 cttttccagc gcatccctgg cattcacaac aagatttaaa agtatctgtt ccagatgcga
     8881 tatgtccaca ttgacaacta gggactcttt gggaaggcta agtgtaaggc ttatgttctc
     8941 agggataagc cttttgagta tcttataggt gtcgcccatg acctctttca gatctattgg
     9001 agcaggtgaa taaggtttgt gcctagaaaa gcccaatagc tgtcgcgtaa gtccagaagc
     9061 cctattaaca actctctcta ttccctggat atcttcattt atttgagaat catctggaat
     9121 ttcaagcttt atcatttcca aaaagcccat tatagatgtc aagaaattat ttagatcatg
     9181 agcaacacca cttgtgagtc gtccaatttc ctctctcttt tgtgactgaa acagctcctc
     9241 cctcaacctc ctagattccg tgacatccaa gaattcgcat aggatttgtg cgtcgctacc
     9301 ctcctcgcga aatctggaaa agctgcagat aagatcgagc gatctctgtt cgaagctatc
     9361 ggaaatccta tttgtttgaa tctctctctg tagaatctgg ttttcttcaa ggctttctat
     9421 tatctcgctc cagattatat cattgggcaa tatatccctg acattttcca ctttcatcgg
     9481 attggaaaag ctcgattcaa atactctgtt tctctcaagg attcttcctt cgatatcggt
     9541 gacgcatctt gctgataggc tataagcaaa taaatccttg taacggcgct cactgtctct
     9601 cagctttttt tccatcgatg aatggtatag agccgtctcc acattaacga taagttctcg
     9661 tgtagaaaat ggttttacaa gaatgccaag aggctgcgct tcacgtagtc ttgcgggaat
     9721 acttgtaacg ccatgatcac ttattaccag gcaatataga ccggtctgtc tatgtatcgc
     9781 tactgcagcg tctattatct caggttgatt gtccccaata tcgatgataa aaagttcatg
     9841 ctcgagatta ttgagtttct gatatgcctg ctcgagttct tcatagatgt ctacctctgc
     9901 cagcccttct tgttcaatgc tctttgagat gtctagcgct attaaaatat cggattcaag
     9961 aatggcaact cttggcatgt tgcgggcctc cttgcctcca tcctgcttta gtcgattgtc
    10021 tactgaaggg actttcaaat aattgtagca ctttcggtgg aaaactccaa atggatagtg
    10081 cgttcgaaat atccactaag tctagacttg gccgaaaatt caaggataaa tactttattt
    10141 ttttcatcga gccgaaattt gtaaacggtg atgacgtcgt tttcactaag gcctatttca
    10201 tcggctggct cagcgggaca tacccattgt atagaataga gcccccctac tttagtcgat
    10261 tcttttaatt gagtaagtct agcgccgaac agtccctcaa gaatcgaatt cttgtgaatt
    10321 ctgtcccatg caggtatcaa agaggctcca tctgtatggc ttatctgcct caatctcctg
    10381 actggcttaa agtcgcgctg cgcttctaaa aaacataatg cacctttcgg aacagaagcg
    10441 agttgaaatt gaattgaagc tatgtccttt gtgagtttcc catttataga agtaagtgta
    10501 tcccctgcta tgaatgccga aagagatgga tcaagatcag cgagaaccag tggactctga
    10561 ccactgcctt ttcccatcat taagcctaac cttgcatgaa tgacttcctt tctctcgaat
    10621 agatcaggaa gagacaatga aatccattga acgggcagtg caaagtttat gttctgaaat
    10681 gatggcaatc cagcaaagac catgccgacc aaaactccgt tctcagaaaa aagcgggctg
    10741 cccgagttgc cctgattgac cggaacatcg atctgagcag cctcgccaaa agatattatc
    10801 tttctgctca atgagctaac aacaccagcc gtaatgctgt tttcaagacc gatcggcgag
    10861 ccaacggcaa agacgcgatc tcccgcatga aatctcattc ccggaggagg cagcatgact
    10921 gtgctgcgag catgttctgc tgactgtatc agcgctagat ccaattcttc gtcccagcca
    10981 aggacttttg aaggcaccct aatctgagga ttatcggaag gtcggatact gatacgcgaa
    11041 tatcctttat aggttggatc aatctcgctc tgaacaacat gatagttagt caggtataat
    11101 ccctcagaaa tctggaaagc cgtgccaagc acgcgatctg gaacgctgta tccgccttcg
    11161 accttgaacc cccgatccac atatactgtg accacgctcg acaccgcatc ggctaaccaa
    11221 tcttggtcgg aaacaaggat agatggagag taactcatct ggccagacga gagcgattca
    11281 aaccaggctt ccgcattgac atcattgttc tccttagctc tctcagccca agtaatgaaa
    11341 tattcttttg gatctcttgt ctcgataaga ctcttcaagt tttctataga aaaatcctca
    11401 atcttattga aagaatattc ttcctgctct cctaagtgag cctttgatag cgttgctttg
    11461 cctatgacat aaccatattc tttgaactta ttctcagctt cccagagagc gagctgcaat
    11521 ctcatcgctg gcgatgctat catcgagagc tcttcagata caatttcacc aagagaatca
    11581 taaaaagata catattgaac caaagcatca gcagaaaaag agagccgacg agccgtgaga
    11641 atatcctgac ttgcgatggc ttttttaaag agaaaaccga tattttctac ggcttttcta
    11701 aaaagcacaa ttcctttctc accgatgatg ctattgtcaa aagattggtt tttctctcgt
    11761 tgctgttcga atagattgga aatcacagcg atgcactgtt caggatcggt atctacaagc
    11821 agatctagat ggctaataat tgaatctcca gtagaattgg catttggctc attctcaggc
    11881 aaagatgaac agttgcttag gattgctgct atactcaagg acaaaaaaag aaccagaata
    11941 gcagcttgcc gtgtcttcat tcaattcctc aacatggcgc gatcccctct tccaggagaa
    12001 ttttgccctc aggcaggcca agaagctctc tcacttcttt atcttccagc gtttcaacct
    12061 gcataagctt atctgcaagt ctgactagtc gattcttttc ccgctttaga atattaattg
    12121 ccttgtctaa agcagaatca agaatgcctt gtatcgccgc atctatcctc tgtgccgtcg
    12181 cctctgaata atccttatgt tgtgctattt cccgtccgag gaaaataggc tcttcttctt
    12241 ctccgagaga gattgggccg atttcttcag ccattcccca ttcgcgtacc atcttttggg
    12301 cgatctctgt agcttgctta atatcctgtg cagcccctgt agtggtctcg ccatatacaa
    12361 tcttctctgc ggcatatcca ccataagaaa tcacgatacg atcgtaaagc catccatagg
    12421 ttctggagta tgtatcctta tctggcaaag ataaagccaa gcctagagct cttcccctgg
    12481 gaactatagt caccttatga agcgaatctg cgtgtgcaag aaaataatgc aggagtgcat
    12541 gtcccgattc atgaattgca gtggctcgtt tttcttcctc agcaatcacc aagctcttgc
    12601 gcgctacacc catcaaaatc ttgtctcgtg catcttcgaa atcgtccatc tcgaccagct
    12661 ctttgttttt gcggatcgca aagagagcgg cttcattgac tagattagca agatcggcac
    12721 ccgaggttcc tggggtcgcg cgagagagtt tctccacatc tacatcacca gaagttggaa
    12781 tctggcgcat gtgtattttg agaatctcgg ttcgctcatt tacatctggc attgcaacaa
    12841 caacctgtct atcgaagcga cccggtctca ataatgcggg atccagtaca tcaggcctat
    12901 tggtagctgc cagaacaatg actccatcct ttgtatcaaa tccgtccatc tcaacaagca
    12961 tttgattcaa ggtctgttct ctttcatcat gacctccccc aagtccagat ccccgggtcc
    13021 tgcccactgc atcgagctcg tcgatgaaga gtatgcatgg agcatgtttc ctgccttgtt
    13081 cgaataagtc acgaacccga ctggcaccaa caccgacgaa catttcgacg aaatccgaac
    13141 cagacatatg aaaaaagggg acatttgctt caccggctgt agcctttgcg atgagtgtct
    13201 ttcctgttcc tggcataccc accagaagga cccctttggg tatcttagcg cccattttga
    13261 ggaacttgtt cggattcttg agaaaatcga cgacctccat aagctcatac ttggcttcct
    13321 tttgcccagc gacatcctca aaggtgatct ttttattgga attgctatac attttggctc
    13381 ggctttttcc aaaactaaat gccttattat tgccctgcat ttgtctcatc attatccata
    13441 tcatgataaa gccaaatatc catggagtga gcccaaatag tatctgaaaa ggacttgtgc
    13501 cactgattgc tccggttacg cttactcctt tttcttcaag cttagccatc agctcagcgt
    13561 caaaataggg tattcttgtc gtaaaaggca tttctgcccc actcttgccc ttcaatacac
    13621 cttctatgtc cctctgatcc atgattttga tggactttac ttctccgagt tcaagataag
    13681 ataaaaatga agagtaagaa atctctcgcg aactctcgtc ttgcccaaag aatagaaaga
    13741 caactaatag gacaattatt gacaaaaaca ggacaagagc cgctctattg cctttgggag
    13801 gaatcgacag atccggctta ttcccatctc tattatcgtt ttgatctggc atttatctaa
    13861 aaatctcctt tcctcgatag gaaaatatat agtatttttc ttttttcgca cagcaaccgt
    13921 gaagaataat ccgattttat actaaatctg ccatcaaaag tcattgtaag aatcgccgca
    13981 atcccatatt catcctctag aatagccaca atatgactta tatcagcaga gattttctgg
    14041 tttttcgaag tcgagcccaa gtctttcgat ctatctccat cacctatgtt ttcaccttcc
    14101 tttctatttc gaaaaataaa aggaaaagaa agcggtatcg atagacgcaa aaactgctcc
    14161 ttgtctattg aggcattatc taaagaagga ggttgtacgc tttgagatac taccatcttc
    14221 aatggcccgc attcatactg gcctggctct cctaccctca gaaagaactc atcaaatctg
    14281 aataaaccct taagttgcga aatacttcta tccaaaaatc cggaagaaga attcgccttt
    14341 ccaatgtact tgacaactcc attggagaga tcgaattgac atcttgctgc ttcgactcta
    14401 ggtgctcctt gtagaattgc acgttcaagg gattccagtg caccttgact gacagcatct
    14461 tttaatccca aatgcataaa aaacagcctc aaaattctcc tgcgcaccaa agcttcttct
    14521 tttctgaaga gggcttgatc cagtatggca tccttaccca gacctctcat cacttttggc
    14581 atgctctgac aagcaattga ggtgattaga gtgtttaact cctgggcttg ccaccgataa
    14641 gactcgattg aagatctcca agaaggaaat tccttatcga gtagaggaat gagcacatgt
    14701 cttattctat ttctcaaaaa agagagctcc ctatttgttg agtcttctga ccattcgagg
    14761 ttattttctc ttgcaaatgc ttctagctcg tggcgtctaa aactcaaaag ggggcgaata
    14821 acattaatac cttttgtgct atcgactaat ctcgatgctg aaatgccacc gagagtctcg
    14881 agacgtccac cgcgaacaag acgcagcaag aaggtctcag cctgatcatc ggcttgatga
    14941 gcagtgacaa tcgtcgagat accaaatctt tgtgctgttc ttacaagagc atggtatcga
    15001 taatgccttg ccgccgcctc gatgccacag tttttcctat gtgcatattc agaaatggcg
    15061 ccttgtctta tattaacaat ggttatagga atactataag ccttgcatgt tttttggaga
    15121 aatattctct ctctttcgag ctcttcctta gatcgaagat tgtgacatac atggatggcc
    15181 cttatgttat catgatcgaa aatacgaacc aggatataaa gcagaacgct tgaatcgagt
    15241 cctcccgaaa aggcaattgc caatggctta tgaaaatcta catgcatgtc gaggagaaat
    15301 ttcttgactt tgtattcgag ggttttcatt ctatttgcct tgcccaggct tgcctctgtg
    15361 caccctggtt tctgtcttgc agatccagat gtatcaagcc tagcaataaa aagcccagct
    15421 ttccagcatc tgaagccgga acactggact cttaagaata aattgaagta ttgaaaagct
    15481 atttatgtac agcttcactt gtcgtttctg cagattcctg ctctgaagat gcaacagctt
    15541 ctcctgcttc ctcagcttct ggctccaatg ccttttcagg ctctccatgt gcaaacttta
    15601 ttatcgcaaa tacggtgtca gggttagtaa ccaactttac cccttcaggc aaggctactt
    15661 ccttcagatg aagggcatga ttcgcttcaa gatctgtgat atcgacttcg atcttttctg
    15721 gaaggtgatc ggaatcgcac tcaacttcga tctcatgaat tgaatgctcc agaatcccac
    15781 catctttgac accttttgct acgccattca ggtgaagagg aactctaaca tggatatttc
    15841 tatcgtgcct tacaagatta aaatcaatat gtatgacatt accagaaatt gtctcctttt
    15901 ggatttcctt caatagagtg gcaaattttt tcccttccaa atcgaggtcg atgatcctac
    15961 tctcggtgac acgttttgca acattagcaa actccttgcc ttcgagaaaa accgagatag
    16021 gctcaatccc cttgccatag atctgagcgg gcaatctgcc tttttttcgc tcttgcctga
    16081 gctctccttt agtcagttcc cctctcggcg agactctcaa tgcaatatgt tccatatttt
    16141 cctactcctt gagccatatg ctttatcctg ggacggcagg attcgaacct gcgcatgccg
    16201 gaaccaaaat ccggaggctt
//
LOCUS       DAOMZL010000040        15622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_3402_length_15622_cov_16.363461, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000040 DAOMZL010000000
VERSION     DAOMZL010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 15622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15622
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3402_length_15622_cov_16.363461"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(485..1018)
                     /locus_tag="PLQ30_04765"
     CDS             complement(485..1018)
                     /locus_tag="PLQ30_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01789.1"
                     /translation="MKRSKIFMVACIMIVAILSPLLAQNVTNEVHAEVGEIHALGGIG
                     FGWRGFGVSGGMEYILQKFTIPNFPLSLGVMGIAGMDFGKGFDLSAAAMATLHWGMKA
                     YKDFPEFFRNFDWYLGLGMGVGILSDFGFGISTGGGLSYYVNDKFAIDLHSYYINYFV
                     GGGSGFSETIGIRYAVK"
     gene            1149..1718
                     /locus_tag="PLQ30_04770"
     CDS             1149..1718
                     /locus_tag="PLQ30_04770"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HPW01790.1"
                     /translation="MIRCPWCGSDPVYIQYHDKEWGSPVHDDQIHFEFLLLETQQAGL
                     SWRTVLGKREAYRNAFADFEPQIVAGFREEDIANLLANPKLIRNKRKLEAAVKNACAF
                     CAIQKKYGSFDSWIWHFVEGHPIINHWQDISEIPVQTELSDLVSNEMKKAGFAYVGSI
                     TIYSHLQAIGIINDHLISCFRHAEIAGAK"
     gene            1824..2342
                     /locus_tag="PLQ30_04775"
     CDS             1824..2342
                     /locus_tag="PLQ30_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HPW01791.1"
                     /translation="MLSQRMIDRINLQINKEIYSAYLYLAMAAKMNELGYSGVGKWLT
                     VQYHEEMFHAMKFAEYLHDQNAEVSYAKIDNPEFKEKEIKPLFQHVLAHEESVTASIR
                     EIMELAITEKDYATQILAQWYIDEQVEEEKNATEIIQKIDLLGNSHQGLYLLDSELAK
                     RESSVPLDFNKI"
     gene            2601..4232
                     /gene="thrC"
                     /locus_tag="PLQ30_04780"
     CDS             2601..4232
                     /gene="thrC"
                     /locus_tag="PLQ30_04780"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPW01792.1"
                     /translation="MAVRYTSTRDARIDVSVAEAIVQGMPPDGGLFVPRNVPRLSADA
                     ILEPRLSYAELAWLVLSPWFDWRETELRPLIEQAYSSSGEKSNFDTPEIVLLSNAGSL
                     NCENKYSGEKQSSKLFLLELFHGKTCAFKDLALSLLGGLLSKSLEKCGIDEPVLVLTA
                     TSGDTGSAALSGLGGQQGIRIAVIYPADGTSEIQRLQMTSVPLEDRCVIGLKGTFDDA
                     QRIVKNIFQNAKNLRSPFFGIRLSSANSINIGRLLPQIVYYVKAWRDLYSLGAIDYGG
                     AFDVVVPSGNFGDILAARYAKAMGLPIRRLVCASNSNRILYDLFATGIYDRRRSFNKT
                     ISPSMDILVSSNLERLLYHAFCENSELVSRLMRQLENEGFFLIPQQFAANISDFCAGW
                     ADDLQTLESIRHMWEIHNLLIDPHTAVACKVAHELFSNSQNHQKDRTIMKTQVVNPIV
                     IAATASPFKFPASCCAALQSGLPNPPISISKNDLSRAFELSQATGISVPKPIADLVTA
                     KINHPIIVEESALESVLADFAHKTANSHACRKLTI"
     gene            4240..5325
                     /locus_tag="PLQ30_04785"
     CDS             4240..5325
                     /locus_tag="PLQ30_04785"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HPW01793.1"
                     /translation="MIEIEVPASCANLGPGFDCLGIALSLSNIVRFEPAEKTQIKGCP
                     MEWADEDNLFLRSLRHSCELLQTSVPKISAEIITSIPPARGLGSSASLAVAGAAAALL
                     LSGKTAKSLKDLANTAVGTVTSLGTEVDMMDDAVGDTETEFNLRAYTGTEVDRTCLTR
                     FLHEEKNQRFLLRAATDIEGHADNAAPAIYGGFTAVTFTEKSIVISQNPAPEHWQFIA
                     CIPDFILETSLARKALPDFYSRQDAVHGISHAVLVALAFLKGNLDLLNLACSDRIHEP
                     YRRPLIPDFEIVETACFEQGAKMVCISGSGPTIMAVFETSVPDVASSTTDILNETIET
                     QIAKSATHKWIVLPLRVDNLGIRARKI"
     gene            5354..6010
                     /locus_tag="PLQ30_04790"
     CDS             5354..6010
                     /locus_tag="PLQ30_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPW01794.1"
                     /translation="MNTQNMIKSQDKRFWLRPQFLPTFYYNIPSSFGEILLIWHDTDT
                     PMIHCIYLPKNGVSRIAQAKTDFNLLEPWRKEVPLPQHIEQILQLLLKMLSGHPVQVP
                     LQIIEHSLSNLKLPEFQQRVLLTEATIPFGYISSYKELAKACGRQLSFRAVASSLSHN
                     PLPLLIPCHRIVASNGNLAGYQGGIEMKRSLLENESIPIRSNKVIMEKAKFWDYSSNS
                     "
     gene            complement(6293..6988)
                     /gene="rsmG"
                     /locus_tag="PLQ30_04795"
     CDS             complement(6293..6988)
                     /gene="rsmG"
                     /locus_tag="PLQ30_04795"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPW01795.1"
                     /translation="MDQQQLLARGLAALDFDISSGVLEKLLRYVDELEQWNPVYGFVK
                     AKGQDLIIKHVLDSLGPWRILEDLFSEMDKQFSDNNKLRIADIGTGAGFPGIPLAIAF
                     PQRSFLLIERLEKRTRFLENVICILGLENVEIHQNTVEDEDGNLECAIFRALRPFTDK
                     KLFRTIWKKIKLGGALCAYKGRIMHAKLELTGLLDDPVLSNMAAHAKITPVWVPFLEE
                     ERCLVIARKTLNI"
     gene            7158..8765
                     /locus_tag="PLQ30_04800"
     CDS             7158..8765
                     /locus_tag="PLQ30_04800"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPW01796.1"
                     /translation="MRKLLFVTGGVCSSLGKGIAASSIGALMEARGFNVQMVKIDPYL
                     NVDAGTMSPYQHGEVYVTDDGAETDLDLGNYGRFTTAKLSRSNSITTGQVYKKVIEKE
                     RAGDYLGRTVQVVPHITDEIKERILAVASNPDVDMTIVEIGGTVGDIESVPFLEACRQ
                     LIHELGKQNAISIHVTLVPAVSSGEIKTKPTQHAVKELQEIGIQPDALILRSSKPLSD
                     ELRRKISLFTNVEEEGVISGYDAATIYEVPLIYYGQRLDTFLLRKMNVESRHAELNKW
                     QTVVQAITKPNRKVKIAVVGKYMDLQDAYKSIWEALIHGGIAYHAGIDIERIDSSRLE
                     DPRADLDAIFKDIHGILIPGGFGERGIEGMICAARYAREKNIPYFGICLGMQIMVIEY
                     ARSILGWQDAHSTEFNKSTQHPVVCLLEEQKKMKNYGGTMRLGAYDAVTKVGSNIRAA
                     YGVELISERHRHRYEVNSDIRLELEAHDLRISATTVDGELIEACEWPNHPWSVGVQFH
                     PEFKSRPTAPHPLFREFIGAALSLSRL"
     gene            8827..9402
                     /gene="lptC"
                     /locus_tag="PLQ30_04805"
     CDS             8827..9402
                     /gene="lptC"
                     /locus_tag="PLQ30_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HPW01797.1"
                     /translation="MGVYRREEWLIKCIFSLCLLFALIALLSSCSQVTEENSLAAKED
                     IPDAQFYDLIRNEYREGKLSMQFSAKHASWYETDRRLEVEELAFITYNGDDGSVSAYG
                     DAQHAILYEISGDAEFSGHVHLQSAEGDACFEAFELSYRRSLDVFETPFDAEVLIKIG
                     NQLLLAGNGLLFDVKQQYYHIEKNVHGSVNQ"
     gene            9422..10144
                     /locus_tag="PLQ30_04810"
     CDS             9422..10144
                     /locus_tag="PLQ30_04810"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HPW01798.1"
                     /translation="MKSKIFALILSFMMIFVPFSIGFAQTNPPNVQPSPEPPMQGQQT
                     QPSSGKKPIVFSANTVQGVIAKGKENTILMGSVHVTTGSLTITADRIDLSGEDYVNVV
                     CLGNVTVLDTEKGFSLVAEKLNYHRDTEIGIAQRKVYVNDTKNNTIIEAEWLRFDQKQ
                     SIFEAAVTVQVRKEDMSISSEYALYNRDTEEIWLHGGAIAVTEDGVLKGDVISSSSDW
                     SKLKISGEVSGTITPKETATSQ"
     gene            10141..10872
                     /gene="lptB"
                     /locus_tag="PLQ30_04815"
     CDS             10141..10872
                     /gene="lptB"
                     /locus_tag="PLQ30_04815"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPW01799.1"
                     /translation="MTDAILEVFNLRKSFGPVLAVSDVSFSMKEGEIIGLLGPNGAGK
                     TTVFYIIAGFLKPNSGSVRLNGVIINNLPMYRRAILGISYLPQESSIFRRMTVEENIL
                     AVLEVRSGLSSAARKEKAYSLMDEFGIVHLAKQPAYTLSGGERRRTEIARALAVEPKF
                     LLLDEPFTGIDPIAIREIKSLIHNLSNKRIGILLTDHNVRDTLAITSRAYIINKGTIV
                     ANGQPQSIIENPLVRETYLGHDFEM"
     gene            11135..11914
                     /locus_tag="PLQ30_04820"
     CDS             11135..11914
                     /locus_tag="PLQ30_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPW01800.1"
                     /translation="MRYFDTHAHIGLIYDDPIEQLLVCQKAKLAGVSRIVSICNSLVD
                     FKQVYENLKPAEYVYHAVGVSPSEVNNPGKDWQRFIEEASKLSRVIAIGEIGLDYYHK
                     FGDRRSQIELFIEQLEIAARLGLPVIIHNREAGKDVLDILKDRLPPAGAVLHCYSENA
                     AYAADILDLELDIYFSFAGNLTYRNARNLHETVAFLPLDRILLESEAPFMVPAEYRNK
                     RNMPEYLPSTAQFLADFLNIDVEEVAETTYQNACQFFKLAP"
     gene            11952..13001
                     /gene="dusB"
                     /locus_tag="PLQ30_04825"
     CDS             11952..13001
                     /gene="dusB"
                     /locus_tag="PLQ30_04825"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPW01801.1"
                     /translation="MKKAIPFSMNFDSRNVPFFLAPVAGYSDVAFRAICFEMGAALCY
                     TEMISAEALVRGHPKTKLLLARDSSERYFAIQLFGSNPTTLAKAARIAADYKPVVIDL
                     NCGCPVPKIIKAGAGAALLRTPERIGEIVRAMRDQTSVPISIKMRTGWDQDSINYLEV
                     AAIAVQAGVCAITLHGRTRAQGYSGKADWEAIKVLSEQTSVPVFGSGDVLNAGAAISM
                     IRETRCDGVMVARGAIGNPFIFSDLNELDNPKEAEAYSYKPPSLFIENSSCPERTLLG
                     LRNPGIIAKTALHHLEMSARYLGERTACIEFRKHFCAYTRGLPGGSELRAKAVRCSTI
                     DEYKPLIEIIAKAPT"
     gene            complement(13025..14365)
                     /locus_tag="PLQ30_04830"
     CDS             complement(13025..14365)
                     /locus_tag="PLQ30_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01802.1"
                     /translation="MRNEKLYRRCAFRIGIGIFALLIFFLCTACANFAPRALVWTDMP
                     ELIIAAQLFNRENDRYIIDVEYKAFPASEIIKTTKPPSLIIARYMLSNLVMKKFASLD
                     ELFSEYYLDPNDMYPALLNAGKLGSKHVLLPVSFDCELIIENKSETARAGTAMLDPET
                     IRQTSQAFTRIENGKISSMGFSPRWDLGFAENWLLAANVGFTPNEKWKPTSVPAISDD
                     ISSWPVLWNKQNLEKCIEGLLSFNSDIGVSQQDDFAFTYFNMPGYQLVLEGKVLYWPM
                     KASEYFKLPHSIREQLRYRFPAVDHKIILTENSRFIGIPRGSKNRKAALEFARWLFVA
                     TNQEKIWKEMESQQLLPDYVGAFDGFSSIMQVNETIFLRYFPEYGQNPLLPAAIPEPA
                     RIPDYWEDFSRDFLHHWLEAVMSNPESRVAIDQGFRSAFEQYMGIMPDWLISNH"
     gene            14436..15524
                     /locus_tag="PLQ30_04835"
     CDS             14436..15524
                     /locus_tag="PLQ30_04835"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW01803.1"
                     /translation="MQKSFDSIERARINWHSQCLRVRYGEKVWRLGIDAGFTCPHRSV
                     DRLHGGCRFCASDGNVSAYQKSQAFKSKTSVPNTINANVDASNHIDDGSTIEQQIHRA
                     LAFTQRRYKAKAFFLYFQAYTCTNAPVPMLARIYDNAIATFERLYYDAQKQAGMDLQL
                     SNLSKTPLKGIIVSTRPDCFDIAKAELLASYRKQGFEVWVEFGLQSMHEETLALINRG
                     HGIDEFLNAMDIAKKAQLSRTVHLILGLPGESHGSMIETAKFVAQIKPEGLKYHDLHL
                     VKGSAFARSFCSGEIALMHPSRLPALLADCLELMPASTEIMRLSADFRHDETINLHPQ
                     VDKHKLARAVEEELAVRDSFQGQRFEFD"
BASE COUNT         4591 a         3469 c         3329 g         4233 t
ORIGIN      
        1 ggtcttggtg atccttgggt cttggtgatc cttgggggct gattggagtc tgctcggttg
       61 gactgctcgg gtgctcgggg acagaggttg ggggctgctc gggtgctcgg ggacggaggt
      121 tgggaccaag gagaatgtat ggatgagatt aggatttcga ggctacttag atgatgctcg
      181 atgctcggcg gggttctcgg cggggacaga ggtttaatct tcgttagtct tgacggcttt
      241 gagattgagg atagcgccta gtacttggct ttgctttggg gacggaggtt ggatcatgga
      301 tgatcaaaaa tatagccaag aaagatggct aacaaaattc gcttgaagcc ataaatgaac
      361 ccatcaactg atgaccatca taaagcttaa agtctacaaa aaagcacgca actctcaatt
      421 atagctgcgt gcattgtggg gatcaaaagt gatcatgaaa aatatcaata ataaagcaat
      481 gaactcattt cacagcatag cgaatgccga tggtttcgct aaaaccagaa cctcctccca
      541 caaaataatt gatgtaataa gaatgcagat ctatcgcaaa tttgtcattc acatagtaag
      601 atagtccacc tcccgtggag ataccaaaac caaaatctga aaggattccc actcccatac
      661 ctagaccaag ataccaatca aaattcctaa agaattcggg aaagtcctta taagctttca
      721 taccccaatg aagggtagcc attgcagctg cagaaagatc gaaacctttg ccgaaatcca
      781 tgccagcaat tcccatcacg ccaagcgaaa gaggaaagtt cggaattgtg aatttttgca
      841 agatatattc cattccaccg ctgactccaa aacctctcca accaaaacct attccaccca
      901 atgcatgtat ctcgcctact tcggcatgaa cttcatttgt aacgttttgt gcaagcaaag
      961 ggcttaaaat agcaacgatc ataatacaag caaccatgaa gattttgctt cttttcacgt
     1021 ggtcctccta tgctacttaa tctggattag aaatgctagg ctagtagctt ataatttata
     1081 gtatatagca atgactaact ctttatcaac atctctgttt tctttcctaa ttatgatatt
     1141 ctgctctaat gattcgttgc ccatggtgtg gaagcgaccc agtctacatt caatatcacg
     1201 acaaagaatg gggtagccct gttcatgatg atcaaatcca tttcgaattc ttgctactcg
     1261 aaacccaaca agcgggcttg tcctggcgaa ctgttctcgg taagcgcgag gcataccgaa
     1321 atgcatttgc ggattttgag ccacagatag ttgcgggctt tcgcgaggaa gatatagcta
     1381 acctgctcgc aaatcctaaa ctaatacgaa acaaacgaaa acttgaagca gctgtcaaaa
     1441 acgcgtgcgc cttctgtgct attcagaaga aatatggctc ctttgattca tggatttggc
     1501 attttgttga aggccatcca attatcaacc attggcaaga catttccgaa atacccgtac
     1561 agacagagct ttcagatcta gttagcaatg aaatgaaaaa agctggcttt gcatatgttg
     1621 ggtcgatcac aatatactcc catcttcaag ccatcggcat tatcaatgat catcttataa
     1681 gttgctttag acatgcagaa attgcgggcg caaaataaat cctatcttat gcttttcctt
     1741 ttatataaaa aaccttatat ttaaaacgca tggcctagcc atgatatcta atcaaactct
     1801 caaaattagt taggaggaat gctatgttat cacaaagaat gatcgatcga atcaatctac
     1861 aaatcaataa agaaatatac tcagcctatc tctatcttgc tatggctgca aagatgaatg
     1921 agcttggcta ctcaggcgtg ggaaaatggc tcaccgtaca gtatcatgag gaaatgtttc
     1981 atgccatgaa attcgcagaa tacctacatg atcagaatgc cgaagtttca tatgccaaga
     2041 tcgacaaccc tgaattcaaa gaaaaggaaa tcaagcctct cttccagcat gtgcttgccc
     2101 atgaggagag cgtgaccgca agtatccgtg aaatcatgga attggctatt acagagaagg
     2161 attatgcgac gcagatactc gctcagtggt atatagacga gcaggtagag gaagaaaaga
     2221 atgctacgga gataatccaa aagatagatc tccttggcaa ttctcatcag ggactatatt
     2281 tgctagattc ggaactagct aaacgagaat cttcagtgcc acttgatttt aataaaatat
     2341 gagcatcaag cgcagggcta atgatctatc atagccctta tacaaacaac tcagagatat
     2401 caacagatga acaaaactag aagagcccaa tacttaggag cctaagctgc taaaaggtag
     2461 tgattaatta aatttagctt gaaaagctaa cggcaagaag actggttttc cgtagatttt
     2521 tcctatcaga aaggcatatg gcaaaaggcc aagcctagca tgggcttttc tcattctcag
     2581 tagttttgta tgctctatac atggccgttc gttatacgag tactcgagat gcccgaatcg
     2641 atgttagcgt cgctgaagca attgtgcagg gcatgccgcc agatggtggc ctttttgtac
     2701 ctcgaaatgt ccctagatta agcgcggatg ctattcttga gccaagactc agctatgctg
     2761 agctcgcatg gctcgtcctt tcgccctggt tcgattggag ggagacagag ctccgtcccc
     2821 ttatcgaaca agcgtattct tcctctggcg aaaaatcgaa tttcgataca ccagaaatag
     2881 tcttgctatc aaatgcaggt tcgctaaact gcgaaaacaa atactctggc gaaaaacaat
     2941 cgagcaagct ttttctgctc gagctttttc atggcaagac atgcgctttc aaagaccttg
     3001 cactaagcct ccttggagga ctattgagca aaagcctcga gaaatgtggc atagatgagc
     3061 ccgttctcgt acttacggca acatcggggg atactggatc cgctgctctt tcagggcttg
     3121 gtggacaaca aggaatccgg atagccgtga tatatccagc agatgggaca agcgaaatcc
     3181 aaaggctcca gatgacctct gtccctcttg aagatcgatg tgttatcgga ctaaagggaa
     3241 ctttcgatga tgcccagcga atagtaaaaa atatctttca gaacgcaaaa aatcttaggt
     3301 ctcccttttt tggaattcga ctcagctctg ccaactccat caacataggg aggcttctgc
     3361 ctcaaatcgt atactatgta aaagcatggc gggacttata ttctttagga gcgattgatt
     3421 atggcggggc ctttgatgtg gttgttccaa gtggcaattt cggtgatatt ctcgctgcaa
     3481 gatatgccaa ggcaatgggg cttcctattc gtaggctggt ctgtgcttcc aattcgaata
     3541 gaattctcta tgatcttttt gcgacgggca tctatgatag aagaagatca ttcaacaaaa
     3601 cgataagtcc ttcaatggat atcctcgtct caagcaatct agagcgactc ctttatcatg
     3661 ctttttgcga aaactccgag ctcgtatcga ggctgatgcg acaactcgaa aacgaaggct
     3721 tcttcctaat tccacagcaa tttgctgcta atatatctga cttttgcgct ggatgggccg
     3781 atgatcttca gacactcgag agcattcggc atatgtggga aattcacaat ttgcttatcg
     3841 atcctcacac tgcggttgct tgcaaggttg ctcacgaact tttcagtaat tctcaaaatc
     3901 accaaaagga tcggacaatc atgaaaactc aggtagtcaa tcctatcgta atcgccgcaa
     3961 ctgcgagtcc tttcaagttt ccagcatcct gctgtgcggc tttgcagagt ggtttgccaa
     4021 atccgccgat ctccatctcc aaaaacgatc tttcccgagc ctttgagctt tcgcaggcta
     4081 cgggtatctc tgtccccaaa cctatcgctg atcttgtcac cgctaaaata aatcatccta
     4141 taattgtgga ggaatcagcc ctagaatcag ttctggctga ttttgctcat aagactgcta
     4201 actcacatgc ctgcagaaaa ttaacgatat gaggttctca tgatagaaat cgaagtccct
     4261 gctagctgcg caaatcttgg cccaggtttc gattgccttg gaatagcgtt gtctctttcg
     4321 aatatcgtgc gtttcgaacc agctgagaaa actcagataa aaggctgccc catggaatgg
     4381 gctgatgagg ataacctctt cctgcgctct ttgcgccata gctgtgagct attgcaaacc
     4441 tctgtcccca agatctcggc cgaaatcatt acgagtattc ctcccgcaag aggtcttggc
     4501 tcaagtgcat ctcttgctgt agctggtgct gctgcggcat tgcttctttc aggaaagaca
     4561 gccaagagtc tcaaagatct agctaatact gcagttggta cagtaaccag cctggggaca
     4621 gaggttgata tgatggacga cgcggtcggt gacacggaga cggagtttaa cctaagagct
     4681 tatacgggga cagaggttga tcggacatgt ctgacacgct ttctgcacga agaaaaaaat
     4741 cagcgttttc ttcttagagc agcaaccgat atcgaagggc atgcagacaa tgctgccccg
     4801 gctatctatg gtgggtttac agctgtaaca ttcaccgaga agagtatagt gatcagccag
     4861 aatccagccc ctgagcattg gcagttcata gcctgtatcc ccgatttcat tctcgaaaca
     4921 agcttagcac gcaaggctct tccagatttc tattcaagac aagatgcagt tcacggtatt
     4981 tctcatgcag tgcttgtagc tctagctttt cttaagggga atctcgatct tttaaacctt
     5041 gcatgttcgg atcgaatcca cgaaccatat cgtcgacctc ttattcctga tttcgaaata
     5101 gtggaaactg catgcttcga acaaggtgca aaaatggtct gtatctcagg ctctggccct
     5161 actatcatgg cagtctttga aacctctgtc cccgatgtcg catcaagcac tacagatatc
     5221 ctgaatgaga cgattgagac acaaattgca aaaagcgcta ctcacaaatg gatagtattg
     5281 cctttaagag tcgacaatct gggaattagg gctcgaaaaa tctgaaaagg tgtttctggg
     5341 agggattctc ataatgaata ctcagaatat gataaaaagc caggacaagc gtttttggct
     5401 aagacctcaa ttcttaccaa ccttctacta taatattcct tcttcttttg gagaaatcct
     5461 gcttatctgg catgatacgg atactccaat gattcattgt atttatcttc ccaaaaatgg
     5521 ggtttctcga atagctcaag ctaagactga tttcaatcta ttggagccat ggagaaaaga
     5581 agttccgctg cctcaacata ttgaacagat tttgcaattg cttttgaaga tgctttcagg
     5641 ccatccagta caggtacccc ttcagatcat tgagcattct ctttctaatc tcaaactacc
     5701 cgaatttcaa caaagggtgc tcttgaccga agccaccatc ccctttggtt atataagcag
     5761 ctataaggaa ctcgcgaagg cttgtggaag acagctctct tttagagcgg tagcaagctc
     5821 actctcacat aatccacttc cccttctaat tccctgccat cgcatagtgg catcaaatgg
     5881 gaatcttgca ggatatcaag ggggaattga aatgaagcgt tcacttctcg aaaacgaatc
     5941 cattccaatc cgctcgaaca aggtgattat ggaaaaggca aagttttggg attattcctc
     6001 aaattcatga atggactgtt gctctttgtc tgtcaaagct gccttgcctt ctttagcacc
     6061 ctccttaggg cgttcgaata tttctgataa ttaatctatc aaacctccgt ccctaaaaag
     6121 atcacacaga gatcgcggat ctttagacac gattcccttt ttgacctcag agatttctta
     6181 taaacctacc atggatctta ttaatccttt tgaaacctct gtccccacat acccccgcat
     6241 acgcctacat aacccattta cgcgcattaa gtacacgctt ccaacaacat ccttaaatat
     6301 ttagggtttt cctcgctata actagacacc gctcttcttc aaggaatgga acccacactg
     6361 gagtaatctt ggcatgagct gccatattgc taagcacagg atcatccaag agtcctgtca
     6421 attctagctt tgcatgcata atgcgccctt tataggcgca taaggctccg cccaatttaa
     6481 tctttttcca aatggttcga aataattttt tgtcagtgaa tggtctcaat gccctgaata
     6541 tggcacattc caaatttccg tcttcatctt ctacagtatt ttgatgaatt tctacatttt
     6601 caagtcctaa tatacaaata acgttctcca gaaaacgggt tcttttctct agtctttcaa
     6661 tcaaaagaaa ggatctttga ggaaacgcaa tggctagtgg aatgcctggg aatcccgctc
     6721 ctgtcccaat atcagcgatg cgcaatttat tattatcgga gaattgcttg tccatctcac
     6781 tgaatagatc ctccaatatc cgccaaggac caaggctgtc caagacatgc ttaataatca
     6841 aatcttgtcc tttcgccttg acgaagccat aaacaggatt ccattgctcc agttcatcga
     6901 cataacgcaa taatttttca agaaccccac tggaaatatc gaagtcgagt gcagccaagc
     6961 cccgcgctaa taattgttgt tgatccatct gattttatta tatcattaga agcttcgcgc
     7021 ataaatcgat gaaataccca atattcatag ccatagttca gcaagataat cttaatcgat
     7081 ccatttttag tccttattcc tattcttttt tctggaggat tgacagcaag tcccgcaatt
     7141 tgggatacta aaaacccatg agaaaactat tattcgtaac gggaggcgtg tgctcatccc
     7201 tcggaaaagg gattgcagcg tcttccatcg gtgctctcat ggaagctcgc ggttttaatg
     7261 tgcagatggt taaaatcgac ccctacctaa atgtcgatgc cggcacaatg agcccttatc
     7321 aacatggcga agtctatgtc actgatgatg gggctgaaac cgatctagac ttaggcaatt
     7381 atggaagatt cacaactgca aagctgagca gatcaaactc cattacaaca ggtcaagtct
     7441 acaaaaaggt catcgaaaaa gagagagccg gagactatct cggccgcaca gtacaggtcg
     7501 ttccacatat cactgacgaa atcaaagagc gcatactagc cgttgcaagc aatcctgatg
     7561 tagacatgac gatcgtggag attggaggta cggtgggcga tatcgaatcg gtgccatttc
     7621 ttgaagcctg ccgacagctc attcacgaat taggcaaaca gaatgcaatt tcaattcatg
     7681 tcaccctggt cccagctgtc tccagcggcg agatcaagac aaaaccaaca cagcatgccg
     7741 tcaaggaatt gcaggaaata ggaatacagc ccgatgcact tatccttcgc tcgtcaaaac
     7801 ccctgagcga tgaactacgc cgcaagatct cgctcttcac caatgtagaa gaagaaggag
     7861 tcatcagtgg ttacgacgca gcgacaattt acgaagtacc tctcatctat tacggccaga
     7921 ggctcgatac attcctgcta cgaaaaatga atgtggaaag ccgccacgcg gagctgaaca
     7981 agtggcaaac ggtcgttcag gcaataacaa agcccaatcg taaagtcaag atagcagtag
     8041 ttggcaaata catggatctg caagacgctt ataaatcgat atgggaagcc ttgatccatg
     8101 gaggtatcgc ataccatgcc ggcatagata tagaaagaat cgattcctcg cgcctcgaag
     8161 acccgcgagc agacctagat gctatattca aagacatcca tggaattttg ataccagggg
     8221 gttttggtga gcgaggaata gaaggcatga tatgcgctgc tagatatgca agagaaaaga
     8281 acattccata ttttggcatt tgtctaggta tgcagatcat ggtcatcgaa tacgcgcgtt
     8341 caatactagg atggcaagat gctcattcaa ctgagttcaa taaatccact caacatcctg
     8401 ttgtctgctt gcttgaggaa caaaagaaaa tgaaaaatta tggcggaacc atgagactcg
     8461 gcgcatatga tgcggttaca aaagttggtt caaacattcg agctgcttat ggtgtcgaat
     8521 tgatcagcga gcgccatcgc catcgatatg aggtcaatag tgatattcgt ctcgaactcg
     8581 aagcccatga tctacgcata agcgcgacaa cagtggatgg agaactaatc gaagcatgcg
     8641 aatggccaaa tcacccgtgg tcagtaggcg ttcaatttca tccagaattt aaatcgaggc
     8701 ctacagctcc tcatccacta tttagagaat ttatcggagc agccttgagc ctctctagat
     8761 tatagtacaa actaaagagt gctttgccca tcaaggagca aggcagggat ataggtgttt
     8821 taggatatgg gtgtttatag aagagaagaa tggctgatta aatgcatttt ttctctttgt
     8881 ctattattcg ccttgattgc tctattgtct tcctgtagtc aagtaactga agagaattcc
     8941 cttgctgcta aagaagatat tccagatgct caattttatg atctcataag aaatgaatat
     9001 cgggaaggaa agctatccat gcaattctcc gcaaaacatg cttcctggta tgaaacagat
     9061 aggcgcttgg aggttgaaga gcttgctttc atcacctaca atggcgatga tggctctgta
     9121 tcagcttatg gcgatgcgca acacgctata ttatatgaaa ttagtggaga cgcagagttc
     9181 tcaggccatg tgcatctgca gagtgctgaa ggcgatgctt gctttgaagc ttttgaactt
     9241 agttatagaa gaagccttga tgtattcgag acaccctttg acgccgaagt tctgataaaa
     9301 ataggcaatc agctccttct tgctggcaat ggccttcttt tcgatgttaa gcaacaatac
     9361 tatcatattg agaaaaatgt tcatggttca gtaaatcaat aatatgaggt agaatgtcgg
     9421 catgaaatcg aagatttttg cattgattct cagctttatg atgatattcg taccattcag
     9481 tattggcttt gctcaaacaa acccgcccaa tgtgcagcct agccctgaac cgccaatgca
     9541 aggtcaacag actcagcctt cgagcggcaa aaagccaatc gtctttagcg ctaatactgt
     9601 ccagggagtt attgcaaaag gcaaagagaa taccattctt atgggatccg tacatgttac
     9661 gacaggaagc ttgaccatta ccgcagatcg catagatctt agcggcgaag attatgtcaa
     9721 tgtagtctgc ttgggcaatg taaccgtact ggacacggaa aaaggattct ctctagtcgc
     9781 cgaaaagctc aactaccatc gagataccga gataggcatt gcgcagagaa aggtttatgt
     9841 aaatgataca aaaaacaaca ccatcataga agctgaatgg ttaagattcg atcaaaaaca
     9901 atcaatcttt gaagcagcgg tcacagtgca ggtacgcaag gaagatatgt cgataagttc
     9961 agaatatgca ttgtataatc gcgatacaga ggaaatatgg cttcatggtg gtgctattgc
    10021 agtgaccgaa gatggcgtcc ttaaaggcga tgtaatttct tcttcgtctg actggtccaa
    10081 actcaagatc tctggtgaag tctcagggac tatcacccca aaggagaccg ccacatctca
    10141 atgaccgatg caatactcga agtattcaac ttaagaaaat cctttggtcc tgtactcgct
    10201 gtctctgatg tcagtttttc catgaaggaa ggtgagataa ttggtttact tggcccaaac
    10261 ggagccggaa agactacagt attctatata atcgcaggat ttctaaaacc aaattctggc
    10321 tccgtacgtc tcaatggagt gattattaat aatctcccca tgtataggcg agcaatactt
    10381 ggaatctcat atctccccca ggagtcgtct atttttcgca ggatgaccgt cgaagaaaat
    10441 atccttgcgg tgctcgaagt ccgtagtgga ttatcctctg ctgcaagaaa ggaaaaagcc
    10501 tattctctta tggatgaatt tgggattgtg catcttgcga aacagccggc ctacactctg
    10561 tctggaggcg aacgccgaag aactgaaata gcaagggctt tagcagtaga gccgaaattt
    10621 cttctcctcg atgaaccatt tactgggata gaccctattg ccatacgcga aataaagtcc
    10681 cttatacata atctctctaa taaaagaata ggtattctct tgactgatca taatgttcgt
    10741 gacacgcttg ctattacatc gcgtgcatat ataataaaca aaggaactat agtagcgaat
    10801 ggccagccac agagtattat cgaaaatcct cttgtccgtg aaacatatct agggcatgat
    10861 ttcgagatgt aggctctccg cttgttgtga tattcgatgc aaacatatca taaaaagcaa
    10921 tagatccgat aaagttgaca aaagctaatt gcaaaaaata ccaggagaag aagttgaata
    10981 atttattgcg acctctaaaa tcttggacta gctcattcat agctttttag aggagcaaat
    11041 tacaaagcat aaatttatgt tgacggcagg gagcgtacat gttacatttt agacagcccg
    11101 catgggctgc ccaccatttt ctatcggaaa aaccatgagg tattttgata ctcacgccca
    11161 catcgggctt atttatgatg acccgattga acagcttctt gtttgccaaa aagctaaact
    11221 tgccggggtc agtcgcatag tcagcatctg caacagcctc gtggacttca aacaggtata
    11281 tgagaacctt aaacctgctg aatatgtcta ccatgctgtc ggtgtatcac catcagaagt
    11341 aaacaatcct ggtaaggatt ggcagcgctt tatcgaagaa gcttcaaagc tttcaagagt
    11401 aatcgcaata ggagaaatcg gactcgatta ttatcacaaa tttggtgata gacgcagtca
    11461 gatagagctc tttatcgagc agctagaaat tgccgcaagg ctaggtctcc ctgtcattat
    11521 ccacaatcga gaagctggta aagatgtgct ggatatccta aaggatcgac tgccacctgc
    11581 aggagcagtt ctgcactgct attcggaaaa tgcagcttat gcggcagaca tcctcgatct
    11641 cgaactggat atctattttt catttgcagg taatctcact tataggaatg cgagaaatct
    11701 gcacgaaaca gttgcattcc ttcctctaga tcgaatatta ctagaaagtg aagcaccatt
    11761 catggtacct gcagagtata gaaataagcg caatatgcct gaataccttc cttccacagc
    11821 gcaattcctg gctgattttt taaacatcga tgtggaagaa gtagctgaaa caacatacca
    11881 aaacgcttgc cagttcttta agcttgcgcc ttagaaatta tattgcttgg ataaagagat
    11941 ttgaaaagca attgaagaaa gcgatcccct tttcgatgaa tttcgactca cggaatgtgc
    12001 cattttttct tgcgccggtg gccggttatt ctgatgtggc tttcagagcc atctgttttg
    12061 agatgggagc tgcgctctgc tatacagaaa tgattagcgc ggaagcactg gtccgaggtc
    12121 atcctaaaac aaaactcctt ctggcccgcg attcttcgga gcgatatttt gctatccagc
    12181 ttttcggttc caaccccaca acattagcaa aggctgcccg aatcgcagct gattataaac
    12241 cagttgtaat agatctgaat tgtggctgtc ctgttcccaa aatcatcaaa gcaggagcgg
    12301 gagcagcatt gctcagaact cccgaacgca taggagaaat cgtacgtgcg atgcgcgatc
    12361 aaacctccgt ccccatttcc ataaagatgc gtactggctg ggatcaagac tcgatcaatt
    12421 atctcgaagt cgcagccatt gccgtacaag caggagtatg cgctataaca ctacatggcc
    12481 gaacacgcgc acaggggtat tcaggcaaag cggactggga agcaattaag gttctctctg
    12541 agcaaacctc tgtccctgta tttggttctg gcgatgtcct aaatgcaggc gcagcaatat
    12601 ccatgatccg cgagacacgc tgcgatggtg tcatggtagc aaggggagct ataggtaatc
    12661 ctttcatttt ctcggatctg aatgagttgg ataatcccaa agaagccgag gcatattctt
    12721 acaaacctcc gtccctgttc attgagaatt cttcttgccc agagagaacc ttattgggac
    12781 ttagaaaccc aggtattata gctaaaactg ctcttcacca tctcgaaatg tctgctagat
    12841 atctcggaga gaggacagca tgcatcgagt ttcgaaagca cttttgcgct tacacaagag
    12901 gtcttcctgg aggaagtgaa ttacgtgcca aagcggttcg ctgctctact attgacgagt
    12961 acaagcccct tattgagata attgccaaag cgcctaccta aattgatctt ttcaaaagat
    13021 aatatcaatg attagaaatg agccaatcgg gcattatacc catatattgc tcaaaagcac
    13081 ttcgaaaacc ctgatctatt gctacccttg attcaggatt agacatcact gcttcgagcc
    13141 aatgatgcaa aaaatctctc gagaagtctt cccaataatc tggaattcgg gcaggctccg
    13201 gtatggcagc tggcaataat gggttttgtc catattcagg aaagtatcgt aggaatatag
    13261 tctcatttac ttgcattata ctgctgaaac catcaaaggc gcctacataa tcgggcaaaa
    13321 gctgctgtga ttccatttct ttccatattt tctcctggtt tgttgcgaca aaaagccatc
    13381 tagcgaactc taacgctgct ttccgatttt ttgagcccct aggaattcca ataaatctcg
    13441 aattttcggt taatatgatc ttatggtcta ccgctggaaa gcgatagcga agctgttccc
    13501 tgattgagtg aggaagcttg aagtattcgc ttgccttcat aggccaataa agcacttttc
    13561 cttcaagaac aagctgatat ccaggcatgt tgaaatatgt aaatgcaaag tcatcttgtt
    13621 gagatactcc tatatcagaa ttaaaagata acaagccttc aatacatttt tcaagatttt
    13681 gcttgttcca taaaaccggc catgagctaa tatcatctga tatggcgggg acagaggtcg
    13741 gcttccattt ttcatttgga gtaaaaccga catttgctgc aagaagccaa ttctcagcaa
    13801 aacccagatc ccaccttgga gaaaatccca tgctgctaat cttgccattt tctatccgag
    13861 taaaagcttg agatgtttgg cgaatcgtct cgggatcaag catggctgta cctgctcttg
    13921 cagtttcgga tttgttctca ataatcaatt cgcaatcaaa agaaacaggt aataaaacat
    13981 gctttgaacc caatttacct gcattgagca aggctggata catatcgttt ggatcaagat
    14041 aatactctga aaaaagttca tccaatgaag cgaatttttt catcacaaga ttcgaaagca
    14101 tgtatcttgc aattatcagg gacggaggtt tggtcgtctt aattatctcg gaagcaggga
    14161 acgctttata ttcaacatcg atgatatacc gatcgttctc tcgattaaaa agttgagccg
    14221 cgattatcag ttcgggcatg tctgtccaaa caagagccct tggggcaaaa tttgcacatg
    14281 cagtgcatag aaagaatatc aatagagcaa atatgccaat accaatgcga aatgcgcatc
    14341 gtctatacaa tttctcgttc ctcacataat cttcatggat agtgctatca tagtaaagaa
    14401 tcaattgcat gcaaagaaga aggtcaagct agattatgca aaaatccttc gactcgatag
    14461 aaagagcaag aatcaattgg cactctcaat gcctaagagt tcgttatggt gaaaaagtat
    14521 ggagattagg catcgatgca ggtttcactt gtccacaccg cagtgtagat cggttacacg
    14581 gagggtgccg tttctgtgct tcagatggaa atgtctcagc ttaccagaaa tcgcaagcct
    14641 tcaagtcgaa aacctctgtc cccaatacca ttaatgccaa cgtcgatgca agcaatcata
    14701 tcgatgatgg atccactatc gaacaacaga tccaccgtgc gcttgctttt actcaacgac
    14761 gttataaggc aaaagccttc tttctctact ttcaggcata cacctgtact aacgcgccag
    14821 tccctatgct cgccagaata tacgacaacg ctatagcgac atttgagcgc ctttactatg
    14881 atgctcaaaa acaagctggc atggatcttc agttatctaa tttgtctaag acacctttaa
    14941 aaggaattat tgtatcaaca agaccagatt gttttgatat agcgaaggca gaactgctag
    15001 catcctatag aaaacagggt tttgaagtat gggtcgaatt tggccttcaa tcaatgcacg
    15061 aggaaacact cgctcttatt aatcgaggcc atggtatcga tgaatttctc aatgccatgg
    15121 atattgccaa aaaagctcag cttagtagaa ccgtacattt aattttagga ttaccgggag
    15181 aatctcacgg gtctatgatt gagactgcta aattcgttgc ccaaataaaa ccagagggat
    15241 tgaaatatca tgatttacat ctagtaaaag gatcagcatt cgcgcgaagt ttctgctccg
    15301 gcgagattgc attgatgcac ccttctcgcc ttcctgctct tctggctgac tgtcttgagc
    15361 taatgcctgc atcgactgag attatgcgtc tgtcagctga ttttcgacat gatgagacaa
    15421 tcaatcttca tccacaagtc gacaaacata aactggctcg agcagttgag gaagaacttg
    15481 cagttcgtga ttcatttcag ggacagaggt ttgaatttga ctgaaagaag agccatcgat
    15541 gatagtcatt tcagcccttg acaattgcca tgctttctcg tattgtcgat tctcgccgac
    15601 tggtctgctg acattggagc gg
//
LOCUS       DAOMZL010000041        15497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_3443_length_15497_cov_17.485365, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000041 DAOMZL010000000
VERSION     DAOMZL010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 15497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15497
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3443_length_15497_cov_17.485365"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3481)
                     /locus_tag="PLQ30_04840"
     CDS             complement(<1..3481)
                     /locus_tag="PLQ30_04840"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPW01804.1"
                     /translation="MKKARVSAMMLILVSFSIVGVYAYDPPKGASLLGRIYGPWTMGG
                     GLSLTEKGDNTWNFLLNPASTSEEELPILALSYTGIADTSAGGPQGWGSAAMAAFSLP
                     KVYGVWNGSVRLFSAPSSMTSMPFGTFATIGGGFSKKISSGLSVGASAWLAMGGNGSF
                     GWGLWSDIGIVEEFGDMGFLKSPRLGFAFSGIGKEFNYETPPIGMSPGSTASSGFPSA
                     FSPSLGFSADLLNDYNLRIMANIDLRAPSFADLEGEIGLGISYRNMLNLRISAAESLY
                     DIQKKTGRSLWPSITISGSIPLGSKDPQNKGVSAILPAFGFMPLYDSLNSFSIGAQIA
                     WRQHDMSPPEVKLELPPSVGGPPVAYISPNDDSKQDKLEIPLSVKDEQGIVAGWLFKI
                     EDRSSGKVVRTITEQSDLPDRIDSFSSIKKGFGYTKQNVPVPNILDWDGRDDEGNPVP
                     DGTYIVSFHAWDDLGKTNANYDSCMNVVVDRSAPIVNAWVLGSEQLLQGFDVMIFSPD
                     GDNSKDTISFRVQGSIEDNWKYEILNENNVAIRTMEIKARSAPRDFVWDGTDDRGKRV
                     PDGYYRFRLSTMDPAGNTGYKIIGISDDKSDWIVVDTSRPAVKVVADKKAFSPKKEGE
                     AGTAQISFELESLDNLVTWKEFIQDQNGKTVWTSSGDGSAPPPESFRFGGRDPSGEVL
                     PDGQYRAGIEMKYRNGYSPLSYSESFILDATPPFATIVLDDIRAIFSPDGDGRRDTLA
                     FSLSGSKEDTWKLSIFDEIGKPVYTREYREALPERFEWDGTDSTGRRVIDNKYTLEIE
                     SVDVAGNEFKVGFEPVVVDTRTPSAFFVLNREAFSPNDDGIADILGVKLKIDSIDGLL
                     DWKLYVSSALNGSVNAEKTGRQIALLQGDGTNSPPTDFQFDGKGEGALLKEGTYRFEL
                     FLEYENGWKTTKLSPEFVIDITAPRAQISRSREFFNPRGETKQSQVIISQIGSREALW
                     IGELRDNQGVLRCKWESKDSQPSEILWDGRTAEGALLPDGIYSYRLFCIDLAGNSFAS
                     EPILIGIDTVVKQSSLSADTMAISPNGDGIQDKMTLSISATAMESVQDWQLAILAGQE
                     RVMEWKGTGKVPQTIIWEGISLTRLPVPDGSYAAIFRAEYPNGDSTEASLGPLIVDRI
                     APQATVSLSRLIFS"
     gene            complement(3559..4566)
                     /locus_tag="PLQ30_04845"
     CDS             complement(3559..4566)
                     /locus_tag="PLQ30_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01805.1"
                     /translation="MNSCVLKGFIMYCAKPSIVVRNVLFAILFFIISFASDAMEPLPY
                     RLFPALYRKENISRIDQNMSLANYQSSGGFLWERVPLVANNTILAYYGSPLSDKMGIL
                     GLYQKEKIAEMLKEMAAQYDQVNGKDGVIPAFYIIYGTCWPGGEIGYLDDKILLDYIR
                     FAQSMGMLVFIDHQIGKYSLRAAMDKILPFLRYPNVHLAIDPEWRTLSPMKEIGSITA
                     SELNDAQNYMDEYIRANDIPGIRMLVVHQFADKMIQSREEVISHRDRVILIHTADGFG
                     APRLKKATYQRNADAENMPVKGFKLFFKSDFALAGFDMPLMSPSEVMQLDPRPSLIIY
                     Q"
     gene            complement(4755..4946)
                     /gene="rpmB"
                     /locus_tag="PLQ30_04850"
     CDS             complement(4755..4946)
                     /gene="rpmB"
                     /locus_tag="PLQ30_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPW01806.1"
                     /translation="MSRVCEICGKGTLYGNNVSHSKNRTSRTWYPNLIKVRTIENGTV
                     RRIKVCARCLKSGKVEKYI"
     gene            complement(5057..6385)
                     /gene="ffh"
                     /locus_tag="PLQ30_04855"
     CDS             complement(5057..6385)
                     /gene="ffh"
                     /locus_tag="PLQ30_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPW01807.1"
                     /translation="MLEKISETFTDVFRTISGKSAISEKNVEDALDRIKIALLEADVN
                     VRIVRRFVNGVLEEARGEKVLRSVTPGQQFIKIVYDRMVSLLGDEKQDLLLRGTDTQS
                     VILLLGLQGSGKTTTAAKLANFLKKRGRRVLLVACDLVRPAAVEQLAVLAGQVGVDIH
                     REELKDPVQVARNALVRAKREGHDVLIVDTAGRLQIDEPLMQELARIRDALSPVESLL
                     VADAMTGQSAVEIAKAFDERIGITGAILTKFDSDTRGGAALSLKSITGKPIKFIGVSE
                     RIDGLEPFYPDRIANRILGMGDIVSLVEKAQEVYDQKEALELEQKISKESFTLEDYLG
                     QIQKMKKMGSVKSMLEMIPGLAGQVDEDRIDLQEMKYEEAILLSMTKKERQNYLIIGP
                     SRRTRIAKGSGTSVAQVNRLLKKFEKSRLMMKKMVKNKGAMAKMFGSGAK"
     gene            complement(6427..6990)
                     /locus_tag="PLQ30_04860"
     CDS             complement(6427..6990)
                     /locus_tag="PLQ30_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPW01808.1"
                     /translation="MTRDEAVALWKRWNSDVSLWHHALSVEAVMRHFAQKYGENAEYW
                     GLIGLLHDVDYEKFPEEHLRHAREILASAGFDDEFVRSVESHGWGICTQVAPELRMEK
                     VLFAIDELTGFIAACAYVRPSRSVLDLEVKSVKKKWGSAAFAAGVRRDVIEQGADMLG
                     IPLDELIGETILALRSVASEIGLKGNL"
     gene            complement(7025..8899)
                     /locus_tag="PLQ30_04865"
     CDS             complement(7025..8899)
                     /locus_tag="PLQ30_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HPW01809.1"
                     /translation="MDNSAQARFRRRILVLVALLTLFAGVVLVRYGLLALEGPENKGL
                     SAEVPIERGRILDRNGRLLAFDIPKFNLAIRKNEIDPYRISKDLSTVAKSIGVQSESL
                     EKKIRESSQNFIYLAKRLDIDMAKPIQAKIEKGDLAGFVLEEINGRIYPEGRLASHLI
                     GFTGEGNIGLEGVEFKLNKELSGVGSDKPRGNDVYLTIDAPLQYALEGIARKAFEQNK
                     AESIFLLAIDVNSGEVLAYVQMPDFDPNTFGSFRESEREDRMSVYSYEPGSVFKIFSM
                     SSILDAGLITPRTRFNCDGAYRRTLPSGEKIVIKDLNSYGMLDLAGILAKSSNAGAGY
                     ASDRISEDEFYSRILSFGFTEKTGIGSPGENPGNLKEPSKWSARTKPTVAIGQEIRVT
                     ALQMITASVAIANGGILLKPNTISRIEDANGNEVYSHEVIAVRRAIAPETSRAIIAAM
                     ESAASLEGTGWRAKVPDVRMAVKTGTAQMIDLKTKAYSDTDFIASTLGILPANNPKLA
                     IYVAIVKPKGESYLGGQIAAPVLRDAAEAAISTLGLPREKSLNVNHAGVVVIQEPPSA
                     SIGSIMPDLKGYSKRQLLPLLLRKDIEVRLEGDGYVVSQSPSPGTAVEAGTRIMLRLQ
                     "
     gene            complement(8905..9333)
                     /locus_tag="PLQ30_04870"
     CDS             complement(8905..9333)
                     /locus_tag="PLQ30_04870"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HPW01810.1"
                     /translation="MKRSSRPEAKKLDSVMITILVVIFMAFLVRFFLLDAAVVEGKSM
                     LPHYRNGQVVLILKAAYGIRGLNGNYWMQWRQPTTGEVVAAISPVSHEIIIKEIGEVR
                     REGKELAYFLIGYNPIESIDSRDFGLIRFDSIIGKVIPQR"
     gene            9430..10635
                     /locus_tag="PLQ30_04875"
     CDS             9430..10635
                     /locus_tag="PLQ30_04875"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPW01811.1"
                     /translation="MKFVGHSENSVLEADKILNKPLVSVLSGEDFIRKQPPKRSKRAN
                     SKSSNQKQRPSSGPLFSPAVVNSDLNSSREKASDDLQRSTRNQFQNLIKLTPRSILLI
                     AVLLSTSVAGVLAASLISHSHPNISLPDENSAQEALMAMLEPEPSSSPIDGSDLPPIP
                     TLLAEKSYTIRRGDTLQTIASRFGVREDTIISANNLSSKSQIQVGKTLKIPNMNGVYH
                     TVKKNESLSYISRAYGVDMTRIADANNISSATIRIGERLFIPNAKLNASVLRNFYGET
                     FIWPVRGQISSPFGYRTNPFSGQRTFHAAIDIVVNRGTAVKATREGKIADTGYNAVFG
                     NYVIIRHADGYQSLYAHLDSILSKKGTSINQGETIGRSGNTGQSTGPHLHFAIFRNGQ
                     AVDPRQYVK"
     gene            10870..11691
                     /locus_tag="PLQ30_04880"
     CDS             10870..11691
                     /locus_tag="PLQ30_04880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPW01812.1"
                     /translation="MTIADNDENLELYFESIRKIPLVTRQEETILASKIAKGDKCALD
                     RLVEGNLRLVVRIAKSMWSPPHSIMDLIQEGNIGLVKAAERFDPSKNVKFSTYAVWWI
                     RQAISRSLVNTGRHIRLPHRKENALRRLSAIQMRASLDLSRCPSEKELAAETGWKETD
                     ISAILNLGDQPVSLEQTNNDELSILDVYEDWRYNPEREIEKSSHTWQSAWLLSTLSER
                     EQEVISKRFGLGCGPEQSLKAVGSIVGCSAETVRHIEKRALESLRHTALQVGLTA"
     gene            11723..12787
                     /locus_tag="PLQ30_04885"
     CDS             11723..12787
                     /locus_tag="PLQ30_04885"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HPW01813.1"
                     /translation="MRKKRTHKGSYYVLLLSSAVLLVIAALIISNSIKSKDSLSKSQD
                     VPYGTPEYVFELPPGYEDLAVIPPEERKGTTSVSKRTASDWDIQAEAQEEIEKVESEE
                     HEPYGSGGPLSVLPGSTTQPTLIIVIDDVGYNNGELDAFLHLPFPITLAVLPQLTHSK
                     DSVIAIHEAGKELILHQPMEALGGNDPGPHAVYCSMDEAIIEKTIAENIDSFPYPPAG
                     MNNHMGSAVTRDSNCMIPILKLAKERGIYYLNSLTAPGTVCAELSRKIGTQYMERDVF
                     LDNNGDRESILAAIEEGKQIARQKGAAVMIGHVWSSKLASTLMEIYPELVEQGYSLST
                     ISQYMKMQAKENSDNAYSGD"
     gene            12768..13787
                     /gene="tsaD"
                     /locus_tag="PLQ30_04890"
     CDS             12768..13787
                     /gene="tsaD"
                     /locus_tag="PLQ30_04890"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPW01814.1"
                     /translation="MHILGIETSCDECAAAVVEDGRHILSNVIMTQIPLHERYQGVVP
                     EVASRAHIEWIMGVVKRAISEAGIEICALEGIAATAMPGLIGSLAVGLSFAKALAWSM
                     KLPFKGVNHMLAHLYAPQLIEPIDYPFIGLLVSGGHTVLCRVEDFDRIEVLGRTIDDA
                     AGEAFDKVAKYYGFGYPGGAAIDKLAENGDEKAFKFPMPSLHKGEHRYDVSYSGLKTA
                     VIYHLSSYLRPGAEITPENIAASFRKTAIDILISRLYRAIHDTGIHTVVVGGGVAANS
                     YLRRKLEEEQDLHVYFPPLSLCGDNGAMVAGIAWHYFRRGEIDGWNLAPNSRFADFKW
                     HKEGN"
     gene            13787..14884
                     /locus_tag="PLQ30_04895"
     CDS             13787..14884
                     /locus_tag="PLQ30_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M17"
                     /protein_id="HPW01815.1"
                     /translation="MYTLAEVAHVAITQCLDVRKSEKLLIISNPETEQEEIARAFAEE
                     ASSMGAQVQFLLQPVKTQEDFAEDKVINAIEESPDVVLSISTEKLGKDKARLASPLLA
                     PDGRSYDHIFNYLLHGLRALRAVWTPGITKDIFLRTVPIDYEEMRRNAAELASLLDQA
                     SAVLVRSPGGTDIVFSVFGRKTMLDDGDFRYPGKGGNLPAGEVFISPALKSAEGTIVF
                     DGSIADIEKDIVIEEPIVCTVRGGYVIGIEGGSEAKKLEAALMRGLSMATGLAGATGM
                     APETALLYGTNARHLGEFGIGLNSAARISGNMLEDEKVMGTIHFAIGSNYDEDAPALI
                     HLDGLVRSPTVVLLMLSGEERTIIEQGVLAV"
     gene            14974..>15497
                     /gene="lysS"
                     /locus_tag="PLQ30_04900"
     CDS             14974..>15497
                     /gene="lysS"
                     /locus_tag="PLQ30_04900"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPW01816.1"
                     /translation="MEQNRDIHWADQTAKKILDTWGEKDLYTCASGITPSGTVHVGNF
                     REMISVELVVRALRDRGKKVRFIYSWDDYDVFRKVPLNMPNPELLEKYLRFPIIMVPD
                     PWGRDESYARHHEVDVEKVLPEVGIYPEFIYQAQRYRSGTYASSMRRALEMREHLKTI
                     LDKYRDEDHKIQGE"
BASE COUNT         4217 a         3762 c         3322 g         4196 t
ORIGIN      
        1 gagaaaatat caaacgagaa agacttactg tcgcctgagg cgctatacga tcgacaatga
       61 gggggcccaa actagcctcg gtggagtctc cattggggta ttcggctcgg aaaatagccg
      121 catagctgcc atcaggaaca ggcaaacgag tcagacttat cccttcccag ataatagtct
      181 gtggcacctt tccggtccct ttccactcca tgaccctctc ttgtccggca agtattgcaa
      241 gttgccaatc ctggacgctc tccatcgccg ttgcgcttat ggaaagggtc attttgtcct
      301 gaatcccatc tccatttggg ctaattgcca tagtatcagc agacagcgag gactgtttga
      361 caacagtatc gatgccaatc aagataggct ccgaggcaaa gctgttgccg gccagatcta
      421 tacagaatag gcgatagcta tagattccat cagggaggag cgctccttcg gcagttctac
      481 catcccagag aatttcagat ggctgactgt ccttagattc ccacttgcac ctcagaacac
      541 cctgattgtc ccgcaactct cctatccata gtgcttctct cgagccaatc tgagagatga
      601 ttacctgtga ctgcttagtc tcgccacgcg gattaaagaa ctctctcgaa cgcgaaatct
      661 gggctcttgg tgcagtgata tcgattacaa attctggact caattttgtc gtcttccagc
      721 cattttcgta ttccaggaag agttcaaagc gataagtgcc ttcttttagc aatgctccct
      781 ctcccttacc atcgaattgg aaatcagtgg gtggagaatt ggttccatca ccctggagaa
      841 gggctatttg tctgcctgtc ttttcagcat tcactgaacc attcaaggca gatgaaacat
      901 agagcttcca gtctagaagc ccatcgatgc tgtcgatttt caatttgaca ccaagaatat
      961 ctgctatacc atcatcattc gggctaaagg cttccctatt gagcacaaaa aaagcgcttg
     1021 gcgttcttgt atctacaaca accggttcaa aaccaacctt aaattcattg cccgcaacgt
     1081 ctacactttc gatttctagc gtatacttat tatcaattac gcgacggcct gtcgaatcgg
     1141 tcccatccca ttcaaagcgc tctggaagtg cttcgcgata ctctcgtgtg tagacaggct
     1201 taccaatttc atcgaagatg ctaagtttcc atgtatcttc tttgcttcct gataagctaa
     1261 aagccagtgt atctcttctt ccatctccat cggggctgaa aatagctcta atatcatcaa
     1321 gaacaattgt ggcgaatggc ggcgtagcat cgagtatgaa agactccgag tagctgagag
     1381 gagaatatcc atttcgatat ttcatctcaa tgcctgctct atactgtcca tccggaagca
     1441 cttctccaga aggatcacgt ccaccaaatc ggaaagactc aggaggaggt gctgagccat
     1501 cgccggagga ggtccagact gtcttgccat tttgatcctg aatgaactct ttccaggtaa
     1561 ccaggttatc taaagactca agttcaaaag aaatttgtgc agtacccgcc tctccttctt
     1621 tctttgggct aaaggctttc ttgtcagcta caaccttaac tgcgggtctg cttgtatcta
     1681 ctacgatcca gtcggactta tcatcagata tgccgatgat tttgtaacca gtgtttccag
     1741 caggatccat agtagagagc ctgaaacgat agtaaccatc ggggactctc ttccctctat
     1801 catcagtacc atcccagaca aagtcgcgcg gagcacttct ggctttgatt tccattgtcc
     1861 taatggcgac attgttttca ttgagaattt cgtacttcca gttatcttct atagagccct
     1921 gaacacggaa ggagattgta tccttgctat tgtcaccatc agggctaaaa atcattacgt
     1981 caaaaccttg caaaagctgt tcgctgccca aaacccatgc atttactatt ggtgcacttc
     2041 ggtccacaac gacattcatg caagagtcat aatttgcatt tgtttttcca agatcatccc
     2101 aagcatgaaa actcactata taagttccat ctggtactgg attgccttca tcatccctgc
     2161 cgtcccaatc caggatattc ggaaccggca cattctgctt tgtataacca aatccctttt
     2221 ttatagaaga aaaagaatcg atacgatcag gaagatcaga ctgttcagta attgtccgta
     2281 ccaccttacc tgaagagcgg tcctcaatct tgaagagcca cccggctaca attccctgtt
     2341 catcctttac cgaaagagga atctctagct tgtcctgttt gctatcgtca tttggcgaaa
     2401 tataagctac gggcggaccg cctaccgaag gcggaagttc gagcttcact tcgggagggc
     2461 tcatatcatg ttgtctccaa gcaatctgag caccaataga gaatgaattc aagctatcgt
     2521 aaagaggcat gaatccaaag gccggaagta tcgctgaaac tcccttgttc tgaggatctt
     2581 tgctgccaag aggtatgctg cccgatatcg taatagaagg ccataaactt cgtccagttt
     2641 tcttttgaat atcatagagg ctttctgctg cagagattcg aagattaagc atgttccgat
     2701 aagaaatacc taatcctatc tcaccctcta gatccgcaaa tgacggcgcg cgcaaatcaa
     2761 tattggccat tatgcgcaaa ttgtaatcgt tgagaagatc tgcactgaat ccaagacttg
     2821 ggctgaaagc agaaggaaaa ccagaagatg ccgtgcttcc tggcgacatg cctattggcg
     2881 gtgtctcata gttgaactcc tttccaattc cgctaaacgc aaaacctaat cttggacttt
     2941 ttagaaatcc catatcgccg aattcttcta caattccaat atcggaccaa agcccccatc
     3001 caaagctgcc atttcctccc attgcaagcc aagccgaagc tcccacggat agaccgcttg
     3061 agattttttt ggaaaatcca cctccaatag tggcaaaggt cccaaatggc atgcttgtca
     3121 ttgaacttgg agcgctaaaa aggcgaacag atccgttcca tactccatag acctttggca
     3181 ggctaaaagc tgccattgct gcgctgcccc aaccttgagg cccaccagcg gaggtatcag
     3241 cgatgcctgt gtaagataaa gcgagaatag gaagttcttc ttcagaagta gaagccgggt
     3301 ttaataggaa attccatgtg ttgtcccctt tttcggtgag cgataatccc ccgcccattg
     3361 tccaaggacc gtaaatacgg ccaagcagcg atgctccctt aggcggatca taggcataga
     3421 ctccgactat actaaaagag accaatataa gcatcattgc agatacccgg gcttttttca
     3481 tctatatccc cttctctata aaaagaaacc ttcaaattca atctatcaca gcaagagccc
     3541 aacttcaggg ctgtctcatc actgatatat gatcaggctt ggtcttggat caagctgcat
     3601 gacttcgcta ggcgacataa gtggcatatc gaatcccgcc agagcaaaat ccgatttaaa
     3661 aaagagctta aaacccttta ccggcatatt ttccgcgtcc gcgtttctct gataggtggc
     3721 tttcttcagc cttggagcac cgaaaccatc agcggtatga ataaggatga cacgatccct
     3781 gtgcgatatc acctcttcgc ggctttgaat catcttatct gcgaactgat gcacaacaag
     3841 catacgtatg ccaggaatat cattcgccct gatatactca tccatataat tttgagcatc
     3901 attgagctca gaggcggtaa tagatcctat ttctttcatg ggactgagcg ttctccactc
     3961 cggatctata gctagatgga cattggggta tcgcaaaaat ggcaagatct tgtccatggc
     4021 cgccctgagg ctgtatttgc ctatctgatg atcgataaat acgagcatgc ccatagattg
     4081 agcaaatctg atgtaatcca aaagtatttt atcgtctaga tatcctatct ctccgcccgg
     4141 ccaacaagtc ccatagatga tgtaaaatgc tggaatcaca ccatccttgc catttacctg
     4201 gtcatactgg gctgccatct cctttaacat ttcggcgatc ttttcttttt gatagaggcc
     4261 gagtataccc attttatcgg aaagtggact gccataataa gcgagaatag tattgtttgc
     4321 cacaagaggt accctttccc acagaaagcc tccactggat tgataattgg caaggctcat
     4381 attctgatca atacgagaaa tgttttcttt tctatagaga gcaggaaaaa gcctataagg
     4441 aaggggttcc attgcatcgc ttgcaaagga aattataaag aagagaattg caaaaagtac
     4501 attgcgcact acgatggaag gctttgcaca atacattata aaccctttca atacacaact
     4561 attcattatt gtatcggcat caacttgagg cttctatagc cccacccaca gggagtctag
     4621 tctatctccg gagcatattt gtaaaccgct tatcaatata ccgtgggaat aaaaaaaccg
     4681 ccatattttg gcggcttttt aaataatcca ttacaaatcg gcaagcattt tgcctaaagc
     4741 cgattgagaa acaactaaat gtatttctcc acttttccag atttgagaca acgagcacat
     4801 acttttatgc ggcgcacagt tccattctca attgtgcgca ctttgatgag attcggatac
     4861 caggttcggc ttgtgcggtt ctttgaatgt gatacattat ttccgtaaag tgtacctttg
     4921 ccgcagattt cacacactct agacattgta gtctccatag aacatagtcg atgcaatata
     4981 ccgaaaatga cgaattatgt aaagtggtca ggatataatt agctcgttta aatatattcc
     5041 tttactctag cttgaattat ttagctccac tcccaaacat tttcgccatg gctcctttgt
     5101 tctttaccat ttttttcatc atcaatctag atttttcgaa tttcttaagg agcctattga
     5161 cttgagctac agaagtgcct gagccctttg caattctagt tcgccttgag ggacctataa
     5221 ttagataatt ctggcgttct ttctttgtca tggacagaag gatagcctct tcatatttca
     5281 tctcctgaag atctatgcgg tcctcatcaa cttgtccggc cagacctgga atcatctcga
     5341 gcatcgactt gacagagccc atcttcttca tcttttgaat ttgacctaaa taatcttcga
     5401 gtgtaaagct ttctttgctg attttttgtt cgagctcaag cgcttccttt tggtcgtaga
     5461 cttcctgggc tttctccaca agcgatacta tgtctcccat gcctagaatg cgattggcga
     5521 tcctatctgg atagaagggt tcgagtccat cgatgcgttc gctgacacca atgaacttta
     5581 ttggtttacc cgtaatggat tttagcgaaa gagcggctcc cccacgcgtg tcggaatcga
     5641 atttcgtcaa gatggcgcca gtgattccaa ttctttcgtc gaatgccttg gcaatttcga
     5701 cagcggactg accagtcata gcatccgcca caagaagact ctctactgga gaaagggcat
     5761 cgcgaattct cgctaattct tgcatgagag gttcatctat ctgaagacgg ccagcagtat
     5821 cgactatgag cacatcatga ccttcacgct ttgcgcgtac caaggcattg cgtgccacct
     5881 gtaccggatc ttttagttcc tctctatgga tatcaacacc gacctgtcca gcgagcacgg
     5941 ctagctgttc aacggccgcg ggtctcacga gatcgcatgc cacgagcaat actcttcgtc
     6001 ctcttttttt aagaaaatta gccagcttag ctgcagtagt ggtctttcct gaaccctgaa
     6061 gacccaagag aaggattaca ctctgagtat cagtccctct taataggagg tcttgctttt
     6121 catcgcccag aagagagacc attcggtcat agactatctt gataaactgc tgtccaggtg
     6181 tgacggaacg aagtaccttt tcccctctgg cttcttctaa cacaccattg acaaagcgcc
     6241 gcacaattct cacatttaca tcggcttcaa ggagcgcgat tttaatgcga tctagagcgt
     6301 cttcaacgtt tttctctgag atcgcagatt tgccgcttat tgtcctgaat acatcggtaa
     6361 aggtctctga aatcttctca agcattgttt ctttccatat atatttgtta ttgaattgcc
     6421 tttgagctac aggttgccct taagcccaat ttccgaagca acgctcctaa gggctagaat
     6481 tgtctcacca ataagctcgt cgagaggtat acccaacata tcagcccctt gctctatgac
     6541 atctctgcgc acaccagctg caaatgccgc agagccccat tttttcttga cagatttcac
     6601 ttccaagtca agcacactgc gcgatggcct gacataggcg caggcggcaa tgaaaccagt
     6661 caattcgtca attgcaaaaa gcaccttttc catgcgaagc tcaggcgcga cctgcgtgca
     6721 tattccccag ccgtgacttt ctacagaacg aacgaattca tcatcaaagc cagctgatgc
     6781 aaggatctcc cgcgcatgtc tcaaatgctc ctcgggaaac ttctcgtaat cgacatcgtg
     6841 caaaagacct atgagtcccc aatattctgc attctcacca tatttctgag caaaatggcg
     6901 catcaccgct tcaaccgaaa gagcatgatg ccaaagagat acatcactat tccatcgctt
     6961 ccatagagca actgcttcat ctctagtcat ttatcatacc tcaagcagga aatgtcttga
     7021 agaatcattg tagccgaagc attattcttg tgccagcctc tacggctgta cccggagatg
     7081 gagactgaga aactacatac ccatctcctt caagccggac ttcgatatcc tttcgcagaa
     7141 gaaggggcaa caattgacgc tttgaataac cttttagatc tggcatgata gagccaatcg
     7201 aggcagaagg aggctcctgg atgaccacga ctccagcatg attgacattg agactttttt
     7261 ctctcggcag gccaagggtg cttatagcag cctcggccgc atctcgcaaa accggtgcgg
     7321 caatctggcc gcccagatag gattctcctt ttggctttac aatagcaaca tatattgcaa
     7381 gctttggatt gtttgccggc aatattccca atgtgctggc gataaagtcc gtatccgagt
     7441 aagcctttgt ttttagatct atcatctgcg ccgttccagt ctttacagcc atacgcacat
     7501 ccggcacctt ggcccgccat cctgtgcctt caagactagc ggctgattcc atagcggcaa
     7561 ttatagctct ggatgtctca ggcgctatgg ctcgccgcac agctataact tcatgtgaat
     7621 aaacctcatt accattcgca tcctcgatgc gggaaatggt gttcggctta agcagaatac
     7681 ctccatttgc tatagcaacc gaagcggtga tcatctgaag tgccgtgaca cgtatctctt
     7741 gtcctatcgc cacagtgggt tttgtgcgtg cagaccactt tgaaggttct tttaggttgc
     7801 cgggattctc accgggggat ccaattcccg tcttttccgt aaaaccaaag ctcaatattc
     7861 gcgaatagaa ctcgtcctcc gagatgcgat ctgaagcgta tccggctcca gcattgctgc
     7921 tcttggcaag aatacctgcc agatccaaca ttccatagga attcagatcc tttattacga
     7981 ttttttcgcc agaaggaagc gtccttcgat atgcgccgtc gcagttgaat cttgtcctgg
     8041 gagttatcag accagcatcg agtatcgatg acatcgaaaa aatcttgaac acgcttcctg
     8101 gttcgtatga atacacagac attctatcct cgcgctccga ttctcggaaa cttccgaaag
     8161 tattcggatc gaaatctggc atctgaacat aggcaaggac ttcaccgcta ttgacatcga
     8221 ttgccaacaa aaaaatggat tcggctttgt tttgctcaaa ggccttgcgt gcgattcctt
     8281 ctaatgcata ctgaagtggc gcgtctatag tgagatagac atcgtttccg cgcggtttgt
     8341 cgcttccaac gcccgaaagc tctttgttta atttaaattc tacgccctct agtccgatat
     8401 tcccttctcc tgtaaaaccg atcaggtgcg aggccagtct cccttccgga tagattcggc
     8461 catttatttc ttcgagcaca aaaccagcaa gatctccctt ttcgattttt gcctggatgg
     8521 gctttgccat atcgatgtcc agtctcttgg cgagatatat aaaattctgg ctcgattccc
     8581 ttatcttctt ttcaagagac tcagactgca cgccgattga ctttgcaaca gtactcaaat
     8641 ccttggaaat tctatatgga tctatttcat tcttacggat ggctagattg aattttggaa
     8701 tatcgaaagc taagagtctt ccatttctat cgagaattct tccacgttca attggcacct
     8761 ctgccgagag ccctttattc tctggccctt ccagagccag cagaccatat cgaaccagga
     8821 caacaccagc aaatagtgtc aacaaagcga caagtacaag gattcttcgc cggaagcggg
     8881 cttgtgcact attatccatc cgagctacct ctgaggaatg acctttccta tgatcgaatc
     8941 gaatcgtatg agtccaaaat ctcttgaatc tatcgattca atcggattat atcctattag
     9001 gaaatacgcc aactcctttc cttctctccg aacctcccct atttctttta tgattatctc
     9061 gtgcgagaca ggggatatag cagcgacaac ttctccggtt gtcggttgtc tccattgcat
     9121 ccagtaattt ccatttagac ctcgtattcc gtaagctgcc ttcaatatca ataccacctg
     9181 gccgttccta taatggggaa gcatggattt cccttccact acggccgcgt cgagcaggaa
     9241 aaacctgacc aagaaggcca tgaaaataac aacaagtata gtaatcatta cgctgtcgag
     9301 ctttttggct tctggacggg acgatctctt catgtatatc tcccttttgc tctcttgcat
     9361 ctcttgcatt ccggctttcg acagcttaga ttaaattatc gaccaaaata tgccgataca
     9421 agagaagcta tgaaatttgt gggtcactct gagaattctg ttctcgaagc agataaaatt
     9481 ctcaataagc ctcttgtttc ggtgctctct ggcgaagact tcattcgaaa gcagccacca
     9541 aaaagatcca aaagagcgaa ttcaaaatca tcgaaccaga agcaaaggcc ttctagtgga
     9601 ccccttttct ctccggcagt tgtcaactcg gatcttaatt cctcgagaga aaaggcctcc
     9661 gacgatctac aacgatccac ccgcaatcaa tttcagaatc taataaagct gacgccacgc
     9721 tctattttat tgattgctgt tctattgtcg acaagcgttg cgggtgttct tgcagcctct
     9781 ctcatatccc attctcatcc taatataagt cttccagatg aaaactcggc acaggaagcg
     9841 ctcatggcaa tgcttgaacc cgaaccatcc tcttcaccaa tcgatggatc ggaccttcct
     9901 ccaattccta ctctccttgc ggagaaaagc tacactatac gtcgtggtga tactctacag
     9961 acaatcgcaa gcagatttgg tgttcgcgaa gatacaataa tctcggcaaa caatctgagc
    10021 tccaaaagcc agatccaggt tggcaaaacc ctcaaaattc ccaatatgaa tggtgtatat
    10081 cacaccgtca aaaagaacga gagtctttct tacatttcga gggcttatgg agtggatatg
    10141 acgcgcatag ctgatgccaa caatatatcc tccgcaacaa ttagaatagg agagcgcctt
    10201 tttataccga atgccaaact aaatgcatcg gttttgcgca acttttatgg cgaaaccttt
    10261 atatggcctg tgcgcggcca gatctcttca ccgttcggat accgaacaaa tccctttagc
    10321 ggacagcgaa cctttcatgc ggctatagat atcgtagtaa acagaggaac tgccgtcaaa
    10381 gcgacccgcg agggcaagat tgcagataca ggctacaatg cggtctttgg caattatgtg
    10441 attatccgac atgcagatgg ttatcagtca ctctatgctc atctggattc cattctttcc
    10501 aaaaaaggaa caagcataaa tcaaggagaa actataggcc gatctgggaa tacaggtcaa
    10561 agcaccggac ctcacctgca ttttgctatc ttcagaaatg gacaagctgt ggatccacga
    10621 caatatgtca aataagaaca tttagaatca agacaaggtg tctatttttt tatacagata
    10681 agatgcattc tggttattga atatcataca tttatttcta aaagagcttc cttccagttt
    10741 catgtttcat gtttttattc aaaaatacct ttatcctaga taaaaatata ggccaattcg
    10801 ttttcttttc cattttggca cactatttgc ttctaattct atggaataat cagtcttgga
    10861 ggaatattca tgaccattgc tgacaatgac gaaaacctcg agctatactt tgaatcgatt
    10921 agaaaaattc cccttgtaac ccgacaggaa gaaacgatcc tggcttcaaa gattgccaaa
    10981 ggagataaat gtgccttaga taggcttgtt gaaggaaacc ttcgtctggt ggtgcgcatt
    11041 gccaaatcaa tgtggtcgcc cccgcattcg atcatggatc ttatacaaga aggtaatata
    11101 gggcttgtaa aggctgccga gcgctttgat ccgtcgaaaa atgtaaagtt ctctacttat
    11161 gccgtctggt ggattcgcca ggctatatcg cgttctcttg taaacacagg ccgacatatt
    11221 cgtttgcctc atagaaaaga aaacgctctt aggcgcctat cagctatcca aatgcgtgct
    11281 agcctcgatt tatccaggtg cccttccgaa aaggagctgg cagcagaaac gggctggaag
    11341 gagaccgata tctccgcaat tctaaatctg ggagaccaac ccgtatctct tgagcagaca
    11401 aacaatgatg agctttcaat tctagatgta tacgaggatt ggcgatataa ccctgaacgc
    11461 gaaatagaaa aatccagtca cacatggcaa agcgcttggc ttctatctac tctttctgaa
    11521 cgagaacaag aagtaatttc gaagcgcttt ggccttgggt gcggccccga acaatcgctc
    11581 aaggccgttg gttctattgt aggatgctcg gcagaaacgg tgcgacatat agaaaagaga
    11641 gctcttgagt ctctaagaca tacagcactt caggtaggat taaccgcttg ataaagcagc
    11701 ctgtccaaga tacgatgagg gagtgaggaa aaagcgcact cataagggtt cttactatgt
    11761 actgctgctc tccagcgctg ttcttttagt aatcgccgcc ctaataatat cgaattctat
    11821 caaatcaaaa gactcactta gcaagagcca ggatgtacct tatggaacac ctgaatatgt
    11881 attcgagctg cctcctggat acgaggatct ggccgttatt ccgcctgaag aaaggaaagg
    11941 aacaacttcg gtctccaaga gaacagcatc ggattgggat attcaggctg aagcccaaga
    12001 agaaattgaa aaagttgaga gcgaagagca cgaaccttat ggttctggtg gtccgctgtc
    12061 agtgttgccg ggcagcacaa ctcagcctac gctcatcatt gtaatcgacg atgtaggcta
    12121 taataacgga gaactcgatg cctttctcca tctccccttt ccgattactc tggccgttct
    12181 tcctcaactt acacacagca aggattctgt gattgcaata cacgaggctg gcaaggagct
    12241 aatacttcat caacccatgg aggctttagg aggcaatgat ccgggtcctc atgccgtcta
    12301 ttgctcaatg gatgaagcaa tcatcgaaaa gaccatagcc gagaatatcg atagcttccc
    12361 atatcctcca gcaggcatga acaatcatat gggctccgcc gtaacccgcg attcgaattg
    12421 catgattcca attctgaaac ttgcgaagga acgtggcata tattatctta attctcttac
    12481 ggctccggga actgtgtgtg cggagctgag tcgaaaaata ggaacgcaat atatggagcg
    12541 cgatgtgttt ctcgataaca atggcgaccg cgaatcaatt cttgctgcaa tagaagaagg
    12601 aaaacaaatt gcccggcaaa agggtgctgc cgttatgatt ggccatgtat ggtcatccaa
    12661 gcttgcctct acacttatgg agatctatcc tgaactggtg gaacaaggtt attcgctctc
    12721 taccataagc caatacatga agatgcaagc aaaagagaac tcagacaatg catattctgg
    12781 ggattgagac ttcctgcgat gaatgcgccg cagcagtcgt cgaggacgga aggcatatcc
    12841 tttccaatgt aattatgact cagattccgc tccatgagcg ctaccaaggt gtagttcctg
    12901 aagttgcaag cagggctcac atagaatgga taatgggcgt ggtaaagcgc gctatatccg
    12961 aggctggaat agagatatgt gctttagaag gcatagcagc aaccgctatg cctggactta
    13021 tcggttctct cgctgtagga ttgtcctttg caaaagctct tgcctggtct atgaagcttc
    13081 cttttaaggg cgtaaatcat atgcttgctc atctctatgc tcctcagctg atcgaaccca
    13141 tcgactatcc atttattggg ctccttgtgt cgggcggaca tacggttctc tgccgtgttg
    13201 aagatttcga tagaatcgag gtgcttggaa ggacgataga cgatgcggcg ggagaggcct
    13261 tcgataaggt cgctaagtac tatgggtttg gctaccctgg cggggctgcg atcgacaagc
    13321 ttgcagagaa tggtgacgaa aaggccttca aattccccat gccttctcta cataaaggag
    13381 aacatcgtta tgatgtttca tattccgggc ttaagaccgc agtaatctat catctttcct
    13441 catatctaag acccggagca gagattacac cagagaatat agctgcaagc ttcagaaaaa
    13501 cagcaataga tatactaata tccaggctat atcgtgcgat tcatgatacg ggtatccata
    13561 cagttgtcgt ggggggtggg gttgcagcca actcatacct aaggcgaaaa ctcgaagaag
    13621 agcaagattt gcatgtctat ttccctcctc tttcgctctg cggagataat ggagccatgg
    13681 ttgctggaat tgcatggcat tattttaggc gaggcgagat agatggctgg aacctagctc
    13741 cgaattctcg ttttgcagat ttcaaatggc ataaggaagg taattgatgt ataccctcgc
    13801 agaggttgcc catgtagcca ttactcagtg tctggatgta agaaaatctg aaaagctctt
    13861 gattatctcc aatcctgaga ccgaacaaga agagatagcg agagcttttg cagaagaggc
    13921 ttcttcgatg ggagcgcagg tgcaattctt gcttcagccc gtcaaaaccc aagaggattt
    13981 tgcagaagat aaagttatca acgcaataga agaaagccca gatgtagtgc tgtctatttc
    14041 gacagaaaag ctaggcaaag ataaagccag gcttgcatcg cctcttttgg ctccagatgg
    14101 ccgctcttat gaccatatct tcaattatct attgcacggc ctcagggcac tgcgcgcagt
    14161 ctggacacct ggaattacaa aggacatatt cctaagaacc gtgccaatag attatgaaga
    14221 aatgcgtagg aacgcggcag aactcgcaag tctcctcgac caagcaagcg cagtccttgt
    14281 ccggagtcct ggaggcaccg atattgtatt ctcggtattc ggacgcaaga cgatgctgga
    14341 tgatggcgat tttcgctatc cgggcaaagg agggaatcta cctgccggtg aggtttttat
    14401 ttctccggct ctgaaatcgg cagaaggtac gattgtgttc gatgggtcta ttgcggatat
    14461 agaaaaagat attgtcatcg aggagccaat agtgtgcact gtacggggcg gctatgttat
    14521 cggcatcgaa ggcggaagcg aagcaaaaaa attagaagca gccctgatgc gagggctttc
    14581 aatggccacc ggcctcgcag gtgcgacagg aatggctccg gagacagcgc tattatatgg
    14641 cacaaatgct cgccacctgg gagaatttgg tattggactc aattctgctg ctcgtatttc
    14701 gggcaatatg ttagaagatg agaaagttat gggtaccatt cattttgcaa ttggatcgaa
    14761 ttacgacgaa gatgcgccag ctcttattca tctggatgga ctagtaagat cacccacagt
    14821 ggtgcttctc atgctctctg gcgaagaaag gaccatcatc gaacaaggtg ttctagccgt
    14881 gtaagcccta ttcattgaaa tacgagccct tctaagtttt ccatcagcaa ccgctattct
    14941 acaaaaacat aaatatcgtt atacttccac aacatggaac agaacagaga tattcattgg
    15001 gctgatcaaa cagctaaaaa aatcttggat acatggggcg aaaaagacct gtatacctgt
    15061 gcatcgggga ttactccttc gggaactgtc catgttggaa atttccgtga aatgatatcc
    15121 gtagagctcg ttgtcagagc acttagagat aggggcaaga aggtcagatt catttatagc
    15181 tgggatgatt acgatgtttt ccgaaaagta cctctcaata tgccaaatcc cgaattactg
    15241 gaaaaatacc tcagatttcc gataattatg gtaccagatc cttggggacg cgatgaatcc
    15301 tatgctcgtc accacgaggt cgatgtcgag aaagtgctgc ccgaggtagg aatctaccct
    15361 gagttcattt accaagctca acgatatcgc tcgggcactt atgcatcgag catgcgccgt
    15421 gctcttgaga tgcgcgaaca cctaaagaca atcctcgata aataccgcga tgaagaccat
    15481 aagattcaag gtgaatg
//
LOCUS       DAOMZL010000042        15227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_3526_length_15227_cov_16.058133, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000042 DAOMZL010000000
VERSION     DAOMZL010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 15227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15227
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3526_length_15227_cov_16.058133"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..2286
                     /locus_tag="PLQ30_04905"
     CDS             316..2286
                     /locus_tag="PLQ30_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPW01817.1"
                     /translation="MSIIAASNGHFIDDNGRWLILRGVNLGGSSKVPTTPDGRTHFGE
                     GFYNGADVSFVGRPFPLEEADEHFSRLTNWGQKFLRLVVTWEALEHQAPGVYDIEYLN
                     YIEGIVEAAARHGISLFIDPHQDVWSRWTGGDGAPMWTLEAVGFEPSRLHASGAALLH
                     QEMGALYPEMQWFSNHLRLGCATMFTLFFAGNDYAPGVCARGMPIQDYLQEHYFQAFG
                     LLAKRIASYDNVVGFGSMNEPGEGFIGIRDVRATRSDMLLPGLAPTPWEAMCAGEGLE
                     TSANRVAVKGLRLRSLERVPLGAKSVRAWKEGEVCVWRRVGLWDIENDRPILKQPGWF
                     DSGKSYKAKNRAGKNGAVAGTHSVFNEFYLKPFVERFAEHLANVSGNSKRFMIFVEGY
                     PQGDIPILRPNFFCVNESHWYDSLTLTMKRWTGFLAYNPERQRVVIGSHAVRRYFREA
                     LARIMQHSQEFMSNAPTLLGEFGLPFDLNNRKAYRTGNFKLHEKVLSLYYDALDANLL
                     SATLWNYTADNTHEHGDGWNREDLSVFCSEDGGGRAIAGFVRPYAPAVAGDIVLMQFY
                     QRKGRFILEYKPEHSIKAPSEVFVPMIQFPRGALISASGATFSEPDYHIAKGTKKASL
                     PAGAPGGPSQSLILKIDAEPDAELCRLVISRL"
     gene            complement(2347..3963)
                     /locus_tag="PLQ30_04910"
     CDS             complement(2347..3963)
                     /locus_tag="PLQ30_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW01818.1"
                     /translation="MKSYSNSLALCVAFFMVCSIAGVSAQQAEPVQQPQQGEPSQLPQ
                     QPAPSQKDSLTLAGEWRGTLKVGASSLRIVFHIVDSGTGYVATMDSPDQGARGIPVSG
                     VRIEGKGVVIEVASVGGSYTGTLDAVGNQLEGFWNQSGISFPLTLSRQPSAEIHTEQK
                     SMPMKEPASLAYRVKEVRFNGGAEEVQLAGTLTLPKVNGPFPAVVLVSGSGPQNRDEE
                     ILGHKPFFIIADYLTQRGIAVLRYDDRGVGASTGDYQAATTFDFAADAQAAIDFLARQ
                     EGIDENNIGIIGHSEGAIIASILAAREEISWDGWASANGAGHESTCDTCGAAGFNETR
                     ESSRPAFIVLLAGPGVQGYELLLMQNEAIGRASGLSGEQIIEANNLNRKLYDIVIEER
                     DEQLLRKKLTDVFYEEIDLNPVLSPLDKEAQKAQVPQVIAPLLSPWIRTFLELDPADY
                     LRKVKVPVLALNGSKDLQVPCKSNLEAIRSALSEAGNNSSNFVELEGLNHLFQHATTG
                     LPSEYSEIEEDFAPEALQIMGDWILNIISP"
     gene            complement(3972..4862)
                     /locus_tag="PLQ30_04915"
     CDS             complement(3972..4862)
                     /locus_tag="PLQ30_04915"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HPW01819.1"
                     /translation="MRVLNFGSINIDYVYSVDEFVKSGETKAARSLSVFAGGKGLNQS
                     LAMAKAGLRVLHAGKVGKEGEFLIDTLKNGGVDTKLVEISDVPTGHAIIQVDDRGRNC
                     ILLFGGANQDIGEAYIDRSLLDFGSGDMLILQNEISSISSIIAKAKTKGMTIVFNPSP
                     YSSLVPNYPLELVDVFIMNEIEATCLSGEQDPDRAAGLLVRSFPQALIVITLGEDGSM
                     CARKGEIIRQKAYRVRAVDTTAAGDTFSGYFIAGLVEGMEISKALDLAARAASLCITR
                     KGAADSVPWRKELEKWDFKS"
     gene            5000..9139
                     /gene="pgk"
                     /locus_tag="PLQ30_04920"
     CDS             5000..9139
                     /gene="pgk"
                     /locus_tag="PLQ30_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPW01820.1"
                     /translation="MTPDLKHPLLNTWENILAHLDRVCESDNEQTQIQLLIELLINLR
                     LIGTDSAIPDMRIQKSDIDDIYRQVEKKTRSMLSSVKAPSAKFIYYAREVIKKFPVRQ
                     DAEEMIERIRSDAEAFEESRTEDDPLRGFAGDLRKEVHRRLSPRIITHYLNPYIELAV
                     HRNPEPMVNAGYVALPYTFSPDDEDEQFIDLAIDLLKRLQFLWDYEEGDMATVRQNLR
                     NNLDIFERCFLRAEVEGLLGILNPESLSSADKELDAFKRLASVKFFLKESYLESRIDL
                     YDLILLDLGLGRLIFLLANDLTNNYFAEVSPRNIRDALEVVRELLAISSIKGLRIQNV
                     QLRQNELGELRESSVSDFIKLKHSLEAISSELQQYVQSEIIDEMTGSLNQILEDYKVP
                     TSKLSQIKTRFFNNFIRRTQIHVLSEFVEKVSTAVNKELERQQGEGQLYLRFQRLNDK
                     GSFSECIEEKGIDAYIAPTWRKPEAWLRPFLGGKGNSIIDMAQIGLRVPPAFVLSYPL
                     LAAMSRNTNHIRAGIIDKLRELEQRTGTMLGNGDKPLLLSVRSGALTSLPGMMSTIMN
                     IGLVPRVQAALSKEFGTDFVDTLYLRFLENCKSALDLSRQCPRTRYKDDVAQGAAQKT
                     KLLEDQGSGLCLQRKKMAELESFLVTFLGPSFLTDEEEQLFACIELVYSSRTSKTVDL
                     YSKTLASHETIETAVTIQQVVFGNLNENSLSGVVMTRNPITGEDKLFGEFKIRAQGEE
                     IVMGSLDTLPIEEICPEIASQLLECKRNLIEFYHQDLDIEFTVEDGVLYLLQARAARL
                     GSFAQLMAATDFLKRGIITLDKYRENIGSLELAYANIPLPRADFRFRQWVPPLATGIP
                     INGGVVSGTLIITIERLKEAEKRRESVIFFAVNTKPTDFNIFNLSSAIVTVYPGRTSH
                     AAITAMTLNKACIVGCSDIEIDYEHRKVIFHGAGDIALTEGERITIDGNAGAVYRGVA
                     PISDTCMPVSSIHEAILKAQSAGEAAQIVETMISTKMKALRRESSLRKKSLAEAGDLH
                     KRNVLVRVDANIDLSENSEPEVVDMRVSLMIPVIRDLLEAGATPIICSHRGDPGTHSL
                     QGISREQIYETYSLLPIAQKLESLMGSVVKFHHVSIGSSGLLISRKDIVPDRINMLEN
                     LRYATGEKDNDEAFARSLAELSDSIFVNDAFNVCLRRHASIVGVPRFIQTSIAGPTVL
                     QELMILEKVLNKVERPFIAVFSGDEIEAQYGAMASMLPRVDAMAMILPSNTASLAPFT
                     NSYPAKITVIDPKDPGWLNKSYALGALLENARTILWAGPAGLTSRLGKDLEDLPPYIV
                     SLHRAMGNASLTIICSETEKHFAGKTTENIHISTGPRSFLEYLERLSLPGIVELDDSM
                     R"
     gene            9251..9826
                     /locus_tag="PLQ30_04925"
     CDS             9251..9826
                     /locus_tag="PLQ30_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPW01821.1"
                     /translation="MPRIVDHDKRKREIARSALMLFSKQGYHESSFSDIASACRLSRS
                     NIYNYFKNKDEIFHYAVVELLNSITQNIELIAHQDSLTLAQKLQRIYQLFTNDFGDGK
                     NASIILDLSLRLKRKDTKLVATLDNSAVNLRKNIENLFVSENANLPYVQIAVVTTLFF
                     SLIESSITHSLFTEKAFIQQNIAVILQMLGI"
     gene            9927..11387
                     /locus_tag="PLQ30_04930"
     CDS             9927..11387
                     /locus_tag="PLQ30_04930"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW01822.1"
                     /translation="MNSISQTLGKSAMNIALNYLNKDPEKNIPKIVDLFMKFTFREDM
                     KRQLLSAKEVMNDPNNPYRGLIMRAFYELDPKIRKRFLTNFVFNSGIIGQDKTAVLKV
                     KYNCNIPWTILMDPTSACNMHCKGCWAAEYDKTDSMDYELLDRIIREGKELGIYFYIY
                     SGGEPMLRKDDLIKLAKVHDDCVFLAFTNGTLIDDACAKELAESGNFGLAFSIEGFEN
                     ATDFRRGKGTYQKVVQGMKNMKKYGAPFGFSACYHSKNAEAVGSEEFLDFMIEQGCMF
                     GWIFTYMPLGKDADTSLLATPEQREYMFHWVRKMRDTRPLFLIDFWNDGQYVNGCIAG
                     GRSYLHINAAGDVEPCAFIHYSNVNIKNTSLLDALRSPLFKQYAENQPFNENHLRPCP
                     LLDNSGKLAEMVKAAGAYSTQPIDREDVDNLTAKCVQAAERWAPVAEKLWAGEMPHYL
                     ESKKKSKELQDKNLEVIKKKLKKGKKTKDSNLEAAS"
     gene            complement(11441..11791)
                     /locus_tag="PLQ30_04935"
     CDS             complement(11441..11791)
                     /locus_tag="PLQ30_04935"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPW01823.1"
                     /translation="MFGKHSSAGFHETSPGNRIKTLCYGEHTLLVEVHLDKDADLLRH
                     KHPYEQTGYLVSGKIVMHIEEKAVELKPGDSWCIPMNAEHKVDVLEDSVVIEVFSPTR
                     EEYIKYKNDNDILQ"
     gene            complement(11871..13292)
                     /gene="lpdA"
                     /locus_tag="PLQ30_04940"
     CDS             complement(11871..13292)
                     /gene="lpdA"
                     /locus_tag="PLQ30_04940"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPW01824.1"
                     /translation="MKEREFDFIVIGAGPGGYVAAIRAAQLGLKTAVVEKNLVGGVCL
                     NVGCIPSKSLIHNARLFAEGGKMLQKAGAKIDMSGFDYEPIWKTSRLVAEKLSKGVNF
                     LLKKNKVELIQGTAKLTGASTVEVEGADAAIELRSKAILLATGSRPRIIPGFELDEKR
                     ILSSTGILMKNTLPKRLFILGAGPIGMEFAYIMNAFGVDVTVVELLPRALPLEDEEVS
                     RIIEKEFRNRGVTIYTGANAKSAEIRNNEVVVHVVDEQTKAMDIYADAALVSVGRTPN
                     TDNLGLEQLGIKTDRGYILTGDYHETSCSGIFAVGDITTYPQLAHAASKAGEIVAERV
                     AHLLKGTPNPQERSLDRLHVPSAVYCDPQVASFGLSEASAKEKCIDHQVARFPYRGNG
                     RAVAEDAPEGQVKLVFDRSNGAILGASIIGEGAADLIHEPLLAANAGLRIDDIAALVH
                     AHPTLSETIMEASKVALDRAIHI"
     gene            complement(13471..14433)
                     /locus_tag="PLQ30_04945"
     CDS             complement(13471..14433)
                     /locus_tag="PLQ30_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01825.1"
                     /translation="MISFEECLVRYWRGNDAILLFYVNPPSVIIGRNQNYWREVAPSC
                     TIPVFRRASGGGAVYHDLGNLNWSMIVPRELHSKEEELCLMAKAISKLGVYTHIGARG
                     EIFVSMRDGETEAKISGTARYFGTHNVLHHGTLLISSNLKTLHACLGGIKVLEDNSIP
                     SVPATPVNLVELKPSLTVCEIISMLSLELAGRDPCEISLSEFWNSRTPHLEIALRDLG
                     VLKGYLASTIDYEDFETFRAHFSSIEWIKDRSPPFSILLSSDNRRIVLKIEGGRVSRI
                     LPFEPDDFGAANLAALLQTRFIGLQFDFDTVELIGRVADDEFAW"
     gene            complement(14672..>15227)
                     /locus_tag="PLQ30_04950"
     CDS             complement(14672..>15227)
                     /locus_tag="PLQ30_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01826.1"
                     /translation="LFSCAPKLAQKDLDAANALFAEAQEAKADVYAPDEFMAAQDAKA
                     ALEAELAAQDAKTSGKSYKQANDLIKAFADASTKAKEAASTNKEQVKNEVAQLIVDVD
                     AQYTTVQALAQAAAKDTKKAAKAKLNVKDINAKVAAMNEEIANAKAANDAEDFAAARD
                     KLNAIKATLDELQSTLESAGFAAQ"
BASE COUNT         4088 a         3686 c         3466 g         3987 t
ORIGIN      
        1 agaagacatc cgttttccct tctgcggtat ccttgatggc gatggtctga tccattcgca
       61 catcgtagat aaccttgtcc gcatacgggc cgttcttcac ttgggctgtt gcgctgagca
      121 gcagcaccat agcgatgaac gatacactaa gaaatttttt catgcgtacc tcctttttta
      181 aggtttgcct ttttccattt tagaacgagc ttttagagtt gtaaagagaa gaatcataat
      241 ttgcggcttg cggcgctgta acaacaggcg tattcttata ttgatcatgc aagtaagact
      301 gaagggaaag gtctaatgtc aataatagct gcatcgaatg gacattttat cgacgataat
      361 gggcgctggc tcattctgcg cggggtgaac ctcgggggaa gcagtaaagt gccaacaacc
      421 ccggatgggc gaactcactt tggagaagga ttctataatg gcgcggatgt ctcgtttgtc
      481 ggcaggcctt ttcctctcga agaggcagac gagcattttt caaggctcac aaactggggt
      541 cagaaattct tgcgccttgt ggtgacatgg gaagcgctcg agcatcaagc accaggtgtc
      601 tatgatatcg agtatctcaa ttatattgag ggtatagtcg aagccgccgc tcggcatgga
      661 atatctctct tcatagatcc acatcaggat gtatggtctc gctggacagg aggagatgga
      721 gctccaatgt ggacgctcga agccgtgggc ttcgagccat caaggcttca tgcgagcggc
      781 gctgcactgc ttcatcaaga gatgggagcg ctatatcccg aaatgcagtg gttctccaat
      841 catctgcgcc ttggctgcgc tacgatgttc actcttttct ttgcggggaa cgattatgct
      901 cctggtgttt gcgcccgggg aatgccaatc caggattacc ttcaggaaca ctatttccag
      961 gcttttggcc tgctcgccaa gcgaattgct tcttatgaca atgtagttgg gtttggatca
     1021 atgaatgagc ccggggaagg atttatcgga atccgcgatg tccgcgctac tcgaagtgat
     1081 atgcttctgc ctgggctagc gccaactccc tgggaagcga tgtgcgccgg ggaaggacta
     1141 gaaacgagcg ccaatagagt agctgtaaaa ggcctgagat tgcgctccct ggaaagagtt
     1201 cctttgggcg caaagagcgt ccgtgcctgg aaggaaggag aagtctgcgt atggcgtaga
     1261 gtcggcttat gggatatcga aaacgataga ccaatattga aacagcctgg ttggtttgac
     1321 tccggaaaat catataaagc aaagaataga gcgggaaaaa atggggctgt cgcaggtact
     1381 cattcagtct tcaacgaatt ttatttgaag ccatttgtcg agcgattcgc ggagcattta
     1441 gctaatgtat ctggcaactc aaagcgcttt atgatatttg tagaaggcta tccacagggc
     1501 gacataccca tacttcggcc taactttttc tgcgtcaacg aaagccattg gtatgattcg
     1561 ctgacgctta ccatgaagcg ctggactggt tttcttgcat ataatcccga aagacaaaga
     1621 gtcgttattg gttcgcatgc ggtgcggcgc tatttccgag aggccttggc gcggatcatg
     1681 cagcattcac aagaattcat gagtaatgcc ccaactcttc ttggcgaatt tggcctacca
     1741 ttcgatctga ataatcgcaa agcatatcga accggaaact ttaaactgca tgaaaaggta
     1801 ctctctctat attatgatgc ccttgacgca aatctcttaa gcgcaacgct ttggaactat
     1861 acggctgaca acactcatga gcatggcgat ggctggaaca gagaagacct ttcggttttt
     1921 tgcagcgaag acggtggtgg aagggctatt gcaggttttg tgcgccccta tgcaccagcg
     1981 gttgcaggtg acattgtctt gatgcaattc taccaaagaa aaggccgatt catcctcgaa
     2041 tacaagcctg aacattctat caaagcgcct tctgaggttt ttgttccgat gatacagttt
     2101 cctcgcggcg ccttgatatc tgcatctgga gccacattct ctgagcctga ttatcatatt
     2161 gcaaaaggaa caaagaaggc cagcctacct gcaggcgcgc ctggagggcc ctctcagtct
     2221 cttatactca aaatcgatgc tgagccggat gctgaattgt gccgcttggt aatctcgcgg
     2281 ctttgataga aaaatccaga gcaatctttc gaagcagggt tattgagaaa acctactaga
     2341 gtctttttag ggactaataa tattcaatat ccaatcgccc attatttgca aggcttcagg
     2401 cgcaaaatcc tcctcgattt cgctgtattc cgagggtagc cctgtagttg catgctgaaa
     2461 gagatggttg agcccttcaa gctctacaaa attagaggaa ttgttcccag cctctgataa
     2521 agccgatcta atcgcctcaa ggtttgattt gcaaggcact tggaggtctt tcgatccatt
     2581 cagcgcaagc actggcactt tcacttttct taggtaatcc gcaggatcca actcaagaaa
     2641 agtgcgaatc catggcgaca atagtggcgc tattacttgg ggaacctgag ctttttgggc
     2701 ttctttgtcc aggggagata aaaccggatt caggtcgatc tcttcgtaga aaacatcagt
     2761 cagttttttg cggagcaatt gctcgtctcg ctcctcgatt acaatgtcgt agagcttgcg
     2821 gttgagattg tttgcttcga tgatctgttc tccgctcaag cctgaagctc ggccaatcgc
     2881 ctcattctgc atgagaagga gctcataacc ttgaactcct ggaccagcga gcagcacgat
     2941 gaaagctggt cttgaagact cacgagtctc attgaatcca gcagccccac atgtatcaca
     3001 agtgctttcg tgccctgcac cattggcgct tgcccagcca tcccaactaa tctcttctcg
     3061 cgcagcgaga atcgaggcga taatcgcgcc ctcactgtgt ccgataatcc cgatattgtt
     3121 ctcatcgata ccttcttgcc gcgcgagaaa atcgatggcc gcctgagcat cagcagcaaa
     3181 atcaaatgtc gttgctgctt gatagtcacc tgtggaagct cccacgccac gatcatcgta
     3241 tcgaagtacc gcaatgcctc tctgcgtcag ataatcagcg attatgaaaa aaggcttatg
     3301 ccctagaatc tcctcatctc tattttgtgg accagagcct gaaacaagta ccacggcggg
     3361 gaaaggtccg tttactttgg gcagtgtaag ggtaccagca agttgtactt cttcggctcc
     3421 tccattgaat ctgacttcct ttactcggta agccaatgat gccggctctt tcataggcat
     3481 agacttttgt tctgtatgga tttctgcgga tggctgcctc gagagagtga gaggaaagga
     3541 tatcccagat tgattccaga agccttcgag ctgattacct accgcatcca acgtgcctgt
     3601 gtagcttcct ccaacagatg caacctcgat caccacgcct ttgccctcta tgcgaacccc
     3661 tgacaccgga atgccccttg ctccctgatc ggggctgtcc atcgtggcca catagccagt
     3721 tcccgaatcc acgatatgaa agacgattct caagctcgat gcaccgacct tgagcgtccc
     3781 cctccattcg cccgctaaag ttaggctgtc cttctgcgat ggggcaggct gttggggcag
     3841 ctgagatggt tcgccctgct gaggctgctg aactggctcg gcttgctgag cagatactcc
     3901 agctatgctg cacaccatga aaaatgccac acataatgct aacgaattag aatacgattt
     3961 catattcccc tctaactctt aaaatcccat ttctcaagct ctttgcgcca tggcacagag
     4021 tcagcggctc cctttcttgt aatgcagagg gaagcagcgc gagctgcaag atcaagagct
     4081 tttgaaattt ccattccctc aactaatcca gctataaaat agccagaaaa cgtgtcacca
     4141 gctgccgtcg tgtctacagc gcgcaccctg taagctttct gtcgtataat ctctccctta
     4201 cgagcgcaca tcgagccatc ttctcctagc gttatcacaa tcaaggcttg cggaaaactg
     4261 cgcacaagaa gtccggctgc cctgtctgga tcctgctccc ccgagagaca agtcgcttcg
     4321 atctcattca tgatgaagac atccactagc tcgagaggat aatttggaac aaggctcgaa
     4381 tatggcgatg gattgaagac aatagtcatg cctttggtct ttgccttggc aattatgctg
     4441 gatatggagg atatctcgtt ttgaagaata agcatatcac cggaaccgaa gtccaaaaga
     4501 gaacggtcaa tataagcttc gcctatatct tgattagccc caccaaacaa cagaatgcaa
     4561 ttcctgcctc gatcatcgac ttgaataatc gcatgtcccg taggcacatc agaaatctct
     4621 acaagttttg tgtctacgcc accatttttc agcgtatcaa tcagaaactc cccttctttt
     4681 ccaactttgc ctgcgtgtaa cacccttagc cctgccttag ccattgcaag agattgattt
     4741 agtcccttgc cacctgcaaa aaccgaaagc gaacgtgcgg cttttgtctc acccgacttg
     4801 acgaattcat ctacactgta gacataatct atattgatcg aaccaaaatt gagaactctc
     4861 atctatgtgc tcctgtgcac ttgctggatc catcatagca taagactctg aatcgtgtac
     4921 tgatatcgga tctcgcgccc tgtcttaatt catactttga acattgacaa tccgtcagaa
     4981 tcattcaaat actctaacta tgacacctga tctgaaacat cctctattga acacatggga
     5041 aaacatcctc gctcatctcg atcgagtgtg cgaaagcgac aacgaacaga cgcaaataca
     5101 gcttctcatc gagcttctca taaacctaag gctcattggc acagacagtg ctattcctga
     5161 catgcgaatc caaaaaagcg atatagatga tatctataga caagttgaaa agaaaacgcg
     5221 cagcatgctt tccagtgtca aggctccttc cgcaaaattt atctattacg cgcgcgaggt
     5281 gataaaaaaa ttccctgttc gccaggatgc agaggaaatg atcgaacgga tccgttcgga
     5341 tgctgaggcc ttcgaagaaa gtcgtactga agacgatcct ctcaggggct ttgccggcga
     5401 tctgcgcaag gaagtgcatc gccgcctttc tccgcgtatt atcactcact acctcaatcc
     5461 atatatagaa ctagccgtgc atagaaatcc agagccaatg gtaaatgcgg gatacgtcgc
     5521 tctcccatat acattttcgc ccgacgatga agatgaacag tttatcgatc tggccatcga
     5581 tctcctcaag cggctgcaat ttctctggga ttatgaagaa ggagacatgg caacagttag
     5641 acagaatttg cgaaataacc tcgatatctt cgaacgctgt tttcttcgcg cagaggtcga
     5701 agggctcctc ggtattctga atccggaaag tctttctagt gcggataaag agctcgatgc
     5761 ttttaagcgc cttgcaagcg taaaattctt tctgaaggaa tcgtatctgg aatcgcgaat
     5821 cgatctctat gatcttattc tgctcgattt agggctcgga cgcctcatct ttcttcttgc
     5881 aaatgacctt accaacaact attttgccga agtcagtccc agaaatatcc gcgatgcgct
     5941 tgaggttgta cgcgagttgc ttgcaatttc tagcataaaa ggtcttagaa tccaaaacgt
     6001 acaattgcga caaaacgaac ttggagagct gcgagaatcg tcggtctccg attttattaa
     6061 gctcaagcac agtctcgaag caatctcttc cgaactacag caatatgtcc aatccgagat
     6121 aatcgatgag atgacgggga gcctcaatca gattctggag gattataagg ttccgacctc
     6181 taagctctct cagataaaaa cacggttttt caacaacttt attcgccgta cccaaatcca
     6241 tgtgctaagc gaatttgtcg agaaggtctc caccgctgtc aataaagagc tcgagcgaca
     6301 gcagggagag ggccaactat atctgcgctt tcagcgtctg aatgacaagg gttccttctc
     6361 cgagtgcatc gaagaaaagg gaatcgacgc atacattgca ccaacctggc gcaagcccga
     6421 ggcttggctg cgcccctttc taggcggtaa gggaaacagt atcatcgaca tggcgcagat
     6481 cggccttcgg gttccgccag cctttgttct cagttatcct cttctcgccg ctatgagccg
     6541 aaacaccaat catatcagag caggaattat tgataagttg cgcgagctcg aacaaagaac
     6601 cggcacgatg ttaggcaatg gggacaaacc cttgcttctg tctgttcgat ctggagctct
     6661 cacttcgctg cctggcatga tgtcgacaat aatgaatatc ggactcgtac cccgggtaca
     6721 agcggcacta tccaaagaat ttgggacaga tttcgtcgac actctgtatc tacgatttct
     6781 ggagaactgc aagagcgctc tggatctcag ccggcaatgt cctagaacaa gatacaaaga
     6841 cgacgtcgct caaggcgctg cgcagaagac taaactactc gaggatcagg gctcaggcct
     6901 ttgtctacag cgcaaaaaga tggcagaact cgagtctttc ctggttacat ttttggggcc
     6961 ttcttttttg accgatgaag aagaacagct ttttgcctgt atcgagcttg tttacagctc
     7021 gcgtacttca aagactgtcg acctatattc caagactctc gcctcacatg agactatcga
     7081 aaccgcggtg actattcagc aggtcgtttt tggaaatctt aatgagaaca gcctgagtgg
     7141 tgtagtcatg actcgcaacc ccatcaccgg tgaggacaag ctctttggtg agtttaaaat
     7201 cagagctcaa ggggaagaaa tcgtcatggg ctccctggac actttaccga tcgaagagat
     7261 ctgtccggag atagccagtc agctcttgga atgcaaaagg aatctcatcg aattctatca
     7321 tcaggatctc gacatagaat tcacggtcga ggatggtgtc ttatatctcc tgcaagcgag
     7381 ggcggcaaga cttggatcct ttgcacaact gatggctgcc acggactttt tgaaacgtgg
     7441 aatcataaca ctcgataaat acagggaaaa catcggaagc ttagaacttg cgtacgctaa
     7501 tattccatta ccgagggctg attttaggtt cagacaatgg gttccacccc tggcaacggg
     7561 tataccgatc aacggtgggg ttgtctctgg gacactcatc ataactatcg agcgactaaa
     7621 ggaagcggag aagcgccggg aaagtgtcat tttctttgct gtcaatacaa aaccgacgga
     7681 tttcaatatc ttcaatctct caagtgcaat tgtaacggtc tatccggggc gcacatcgca
     7741 tgcagccatc accgcaatga cgctgaataa ggcctgtatt gtcgggtgct cggacatcga
     7801 aatcgattat gaacaccgta aggttatctt tcatggtgct ggagacattg ccttaaccga
     7861 aggagaacga ataaccatag atggcaatgc aggtgcagtg tatcgaggtg ttgctcccat
     7921 ctccgacaca tgtatgcccg tctccagcat ccatgaagcc atccttaagg cgcagtctgc
     7981 cggagaagca gcccaaatcg tcgagacaat gatttctaca aagatgaagg cactccggcg
     8041 agaatcaagt ctccgaaaaa aaagtcttgc ggaagccgga gatctgcata agcgcaatgt
     8101 gctcgtgcgt gttgacgcaa acatcgacct cagcgagaac tccgagcccg aagttgtgga
     8161 tatgcgagtt tcgctaatga ttcctgtaat cagagacctc ttggaggctg gcgcaacgcc
     8221 aatcatctgc tcacaccgcg gagatccggg aacccatagc ctgcaaggca tatcccgcga
     8281 acaaatttac gagacataca gccttctgcc aatcgcacag aaactggaaa gccttatggg
     8341 aagtgtcgtc aaatttcacc atgtgagcat tggctcatcc ggcttattga tctcaaggaa
     8401 ggatatcgtt ccagatcgga tcaatatgct ggaaaatctc cgatatgcaa cgggagaaaa
     8461 agacaacgac gaagcctttg ctcgttctct tgctgagctg agcgatagca tattcgtcaa
     8521 tgatgcattc aatgtatgcc tgcgccggca tgcaagcatt gtgggtgtgc ctcgttttat
     8581 ccagaccagt attgctggcc ccactgtctt gcaggagctt atgatcctcg aaaaagtgct
     8641 gaataaggtt gagcgaccat ttattgcggt attcagtggc gacgagattg aagcgcaata
     8701 cggcgcaatg gcatcaatgc ttccaagagt cgatgccatg gcaatgattc ttcccagtaa
     8761 tacggcatcc ctggcgccat tcaccaattc ctaccccgct aagataaccg taatcgatcc
     8821 gaaggatcct ggctggctta ataaaagcta cgctctgggt gctttgctcg aaaacgcacg
     8881 aaccattctt tgggcggggc ctgctggttt aacatccaga ttaggaaagg acttagagga
     8941 tcttcctccc tatatagtct cgctgcaccg agcgatggga aatgcatcct tgaccattat
     9001 ctgttcggaa acggaaaagc atttcgcagg gaaaacaact gaaaatatcc acatctcaac
     9061 aggcccccgt tccttcctgg agtatcttga acgactttct ctgcctggta ttgtcgagct
     9121 cgatgactca atgagatagc aaagacatga atcgtcatta atcgttgaca gaagtctttg
     9181 gcctaattat atttgacata gctgtcagaa taaataattg caccaaagta taatttagga
     9241 gacaaagctt atgccaagga tcgtggatca tgataaaaga aaacgagaaa tcgcccggtc
     9301 tgcacttatg ctgttttcta agcaaggcta tcatgaatct agctttagcg atatagcatc
     9361 ggcctgcaga ctaagccgct ctaatatata taattacttc aagaataagg atgagatctt
     9421 tcactatgcg gtagttgaat tgctcaactc gattactcaa aatatcgagc tcatagccca
     9481 tcaggatagc ttgacattag ctcagaagct ccagcgaatt taccagcttt ttaccaacga
     9541 cttcggggat gggaagaacg cctcgatcat cctcgatctt tcgctgagac tcaagcgaaa
     9601 agataccaag cttgttgcca cccttgataa ttctgctgta aatcttcgca aaaacatcga
     9661 gaatctcttt gttagcgaga atgcaaatct gccttatgtc cagattgcag tagttacgac
     9721 tctctttttt tctcttatag agagcagcat tacgcattct cttttcactg aaaaagcatt
     9781 cattcagcag aatatagcag tgatcctgca aatgctggga atatgattgt aggaaaaggt
     9841 gctttgttga aagcgaacat ggagattcct tgtggttata ttgacagagt gctcttgctg
     9901 acaatagaaa gaaagaggag ggaaatatga attccatctc acaaacattg gggaaaagtg
     9961 cgatgaacat cgctcttaat tacctaaaca aggatcctga aaagaatatc ccaaaaatcg
    10021 tagatctatt catgaaattc accttccgtg aagatatgaa gcgccagcta ttgagtgcaa
    10081 aagaggtgat gaatgatccc aacaatccct atcgaggtct cataatgagg gcattctacg
    10141 aactcgatcc gaagattcgc aagcgctttc taaccaattt tgtcttcaat tccggaataa
    10201 tcggccaaga taagactgct gttctgaagg ttaaatacaa ttgcaatatc ccatggacta
    10261 tccttatgga tcccacaagt gcatgtaata tgcactgcaa gggttgctgg gccgcagagt
    10321 atgacaaaac cgattccatg gattatgagc ttctcgatcg aatcatcaga gaaggaaaag
    10381 agcttggaat ctacttctat atctactccg gcggagagcc gatgcttaga aaggatgacc
    10441 taataaaact tgcaaaggtt catgatgact gcgtattctt ggcatttacg aacggtaccc
    10501 tgatagatga tgcttgcgca aaggaacttg cggaatctgg gaatttcgga ctggcattca
    10561 gcatcgaagg ttttgaaaac gctactgatt ttagacgagg aaaaggtacc tatcagaaag
    10621 ttgtccaagg catgaagaac atgaaaaaat acggcgctcc ctttggattt agcgcatgct
    10681 atcattcgaa gaatgcagaa gcggttggta gcgaagaatt tctcgatttt atgatcgaac
    10741 aaggctgcat gtttggctgg atattcacat atatgcccct ggggaaagat gcagatacta
    10801 gtctcttagc cactccagaa cagcgcgagt acatgttcca ttgggtcagg aagatgcgcg
    10861 atacaaggcc gctcttcctc atcgatttct ggaacgatgg ccaatatgta aatggatgca
    10921 ttgcgggtgg aagaagctac ctccatatca acgctgctgg agatgtcgaa ccttgcgcgt
    10981 ttattcacta ttcgaacgta aacatcaaga atacatctct cctggatgca ctgcgctcac
    11041 ccttgttcaa gcaatatgcc gagaatcaac cgttcaacga aaatcacctg aggccatgcc
    11101 ctcttttgga caatagtggg aaacttgcgg agatggtaaa ggctgctgga gcatattcga
    11161 cgcagcccat agatagggaa gatgttgata atctgacggc aaagtgtgtg caagctgccg
    11221 aaagatgggc gcctgtggct gaaaagctct gggctggtga gatgccccac tatctcgaat
    11281 ccaaaaagaa gagtaaagaa ttacaagata agaatctcga agtaatcaag aagaagctca
    11341 agaagggcaa gaaaacaaag gattcaaatc tggaagcggc ttcttgagcc tgtcttcccg
    11401 taaagcgtaa gccaggtact attaaaagaa tgactaatgc tcattgtaga atatcattat
    11461 cattcttgta ttttatatat tcttctcgcg ttgggctaaa aacctcgatg acgacactat
    11521 cctcgagaac atccaccttg tgctcggcat tcataggaat gcaccagcta tcgccaggct
    11581 tgagctccac tgctttttct tcgatgtgca taactatctt gcctgaaaca agatagcccg
    11641 tctgctcata ggggtgttta tgccttagaa gatcggcatc tttatccaga tggacttcga
    11701 cgagtagagt atgttcgcca taacatagag tcttgatacg atttccaggc gatgtctcgt
    11761 ggaagccagc gcttgaatgt tttccaaaca tggtgttcct cccataggaa tttctcgtat
    11821 aataattttc aataataagc atatacaata agcatagaaa tccctttgct ctagatatgg
    11881 atagccctat ccagcgcaac tttcgaagct tccataatcg tctccgacag tgttggatgg
    11941 gcatgcacaa gagccgcgat atcatcgatc ctgagcccag cattcgcggc caggagaggc
    12001 tcatggatca ggtcagccgc gccttcgcct ataatcgagg caccgagaat cgctccgttc
    12061 gatcgatcga aaacaagctt cacctggcct tccggagcat cttctgccac cgcgcgccca
    12121 ttgccccgat aaggaaacct tgctacttga tgatcaatgc acttttcctt tgcactagct
    12181 tcagacagac caaaagaagc cacttgagga tcgcaataga cagcactcgg cacatgaagt
    12241 ctatcaagac tgcgctcttg cggatttgga gttcccttta aaagatgcgc aactctctcg
    12301 gcaacaatct ctccagcctt ggaagctgcg tgcgccagct gaggataggt tgtaatatcg
    12361 cctaccgcaa atatacccga gcaagaggtt tcatgataat cgcccgttag aatgtagccc
    12421 cggtctgtct tgattcctag ctgctcgagc ccaagattat cggtattggg cgtacgccca
    12481 accgaaacaa gggccgcgtc cgcatagata tccatggcct tggtctgttc atccactacg
    12541 tgaacgacca cctcattatt cctgatttcc gcacttttag catttgcacc agtgtaaata
    12601 gtcacacctc gattacggaa ttctttttca ataattcttg agacttcttc atcttctaag
    12661 ggtagtgccc ttggcaagag ctccacaaca gtcacgtcta cgccaaaagc attcattata
    12721 taagcaaatt ccattccaat cgggccagcg cccagaataa agaggcgttt gggaagagta
    12781 ttcttcatca atatgcccgt agaagacaag atgcgctttt catctaattc gaaacccggg
    12841 atgattcgcg gtcttgaacc ggtggcaaga agtattgctt tagacctaag ctctatagcg
    12901 gcgtcagctc cttctacctc gaccgtgctt gctccagtga gcttggccgt tccttgaatg
    12961 agttctactt tatttttctt taatagaaaa ttgactccct tagagagctt ttcggcaact
    13021 aggcgagaag tcttccatat gggctcataa tcaaagccag acatatctat ctttgcaccg
    13081 gctttttgca acatctttcc accttcggca aaaagccgag cgttgtggat cagcgatttc
    13141 gagggaatgc aacccacatt gagacagact cctcctacaa gatttttttc cacaaccgca
    13201 gttttaaggc ctaactgcgc cgcacgaatc gcagcaacat aaccacctgg acccgcaccg
    13261 attacaataa agtcgaattc tctctctttc atgattattg ctcctggaat tccgtcgatt
    13321 gcctttatgg gctatctttg tattttcgta aggtcagcaa ataaggcgga ccaatcttgc
    13381 taccaatgct aaaacggtca tggcttgagg tcaaggagag gctgaagaga attctggcct
    13441 agaatgcgga attttcatga gctctttggc tcaccatgcg aattcatcat ccgcaaccct
    13501 gccaatcaac tccacagtat cgaaatcgaa ttgcagacca ataaacctcg tctgaaggag
    13561 cgcggcaaga tttgcggctc caaaatcatc cggttcgaag ggcagtatcc tggacactct
    13621 gcccccttct attttcagga ctattcttct gttatcgctt gaaaggagta tggaaaaagg
    13681 ggggcttcga tccttaatcc attctataga actaaaatgt gccctgaatg tctcgaaatc
    13741 ctcatagtct atggttgatg ctagatatcc cttcaatact ccgagatctc tcaatgcaat
    13801 ctcgagatgg ggcgtgcggc tattccaaaa ttcggaaagg cttatctcac aaggatccct
    13861 tcctgcaagt tcaagagaaa gcattgaaat tatctcgcaa accgtgagcg aaggctttaa
    13921 ttcaaccaaa ttgaccggag ttgctggcac cgatggaatc gaattatcct ctaatacctt
    13981 gattcctcct agacaggcat gaagagtttt cagattggaa gatataagca atgttccatg
    14041 gtgtaacaca ttgtgagtac caaaatacct agctgtacca gagatctttg cttctgtttc
    14101 accatcgcgc attgacacaa agatctcgcc tctcgctcct atgtgtgtat atactccgag
    14161 cttggaaatc gctttagcca tcaggcacag ctcttcttcc ttcgaatgaa gttccctcgg
    14221 aacaatcatg gaccaattca aattgccgag atcatggtat actgcgcctc ctcccgaggc
    14281 acgtcgaaaa actgggatcg tacaagaagg tgcaacttcc cgccagtaat tttgattcct
    14341 tcctattatc acgctcgggg gattaacata gaaaaggaga atagcatcat ttcctctcca
    14401 atatcttacg aggcattctt cgaaagaaat catatcaggc gggctgtccg ccaggactct
    14461 ataagatcca gcaaatctag gcatgtttcc aatatataag aaatttttgc caattccaac
    14521 gcatttttgt ctctgatacc attaaaccaa aagaccgccc tttaggagcg gtcttttatc
    14581 gaaatgcagg agctatgact tgaatagcaa aacgttgccg acttctttat tctatagtat
    14641 agctcgtgcc actacttgat atactttatt attattgagc ggcaaatccg gcagattcaa
    14701 gggttgactg aagttcatca agagtggctt tgatcgcatt aagcttatca cgagcagccg
    14761 cgaaatcttc agcatcattt gccgcttttg cattggcaat ctcttcattc atcgcagcga
    14821 cttttgcgtt gatatctttt acgttgagct ttgcctttgc cgccttcttt gtgtctttcg
    14881 ccgcggcttg agcaagagcc tgaacagtgg tgtattgagc atcaacatcc acaattagct
    14941 gtgcaacttc gttctttacc tgttccttgt ttgtagacgc agcttctttg gcctttgtac
    15001 ttgcatctgc aaaagctttt ataaggtcat ttgcctgctt ataagacttt ccagaggtct
    15061 tggcatcctg agctgcaagc tcggcttcga gtgcggcttt ggcatcctgg gcagccataa
    15121 attcatctgg agcatagaca tcggctttag cttcctgagc ttcggcgaat agtgcattgg
    15181 ctgcatctag atccttctga gcaagcttcg gcgcgcatga gaacaac
//
LOCUS       DAOMZL010000043        14833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_3671_length_14833_cov_16.526188, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000043 DAOMZL010000000
VERSION     DAOMZL010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 14833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14833
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3671_length_14833_cov_16.526188"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1650)
                     /gene="guaB"
                     /locus_tag="PLQ30_04955"
     CDS             complement(136..1650)
                     /gene="guaB"
                     /locus_tag="PLQ30_04955"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPW01827.1"
                     /translation="MSLEKLLQEGAGLTFDDVLIVPRYSEVLPSEVDISAELVPGIRL
                     KAPVLSAAMDTVTDSRLAIAIARIGGIGIVHRNLSPEAQAAEVYKVKRSESGMISDPV
                     WLPETATLAEAEHLMETYRISGIPVVDPLTGRLVGIITNRDKRFCGPQDMPKPVSEFM
                     TSKNLITAPEGTSIEEAKAIFRRYKIEKLPLVDEEGRLKGLITLKDIVKKEEYPNAAV
                     DARGRLLVGAAVGVGSDLEERVSLLLSRNVDVLVIDTAHGHTKKVIEAIRRIRSLSGD
                     IHVIAGNVVTAEGTQALIQAGASAIKVGVGAGSICTTRIISGVGMPQLSAIYECAQAA
                     KAYGIPIIADGGIRYSGDIVKAIAAGAETVMLGNLLAGLEEAPGELVFFEGRQFKSYR
                     GMGSIGALQGYGRDRYGSGQSDASTDRQLNVRQSGKAVPEGVEGMVPYRGKLSDYMVQ
                     MLGGLRSGMGYAGARTISELRKARMIRITAAAYAESHPHSIVISKEAPNYQKRD"
     gene            complement(1697..3493)
                     /gene="purH"
                     /locus_tag="PLQ30_04960"
     CDS             complement(1697..3493)
                     /gene="purH"
                     /locus_tag="PLQ30_04960"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPW01828.1"
                     /translation="MPIALISVYDKTGLVPFAKRLAACGWRFLASGGTAATLREAKLE
                     PLNIAAYTGSPELLGGRVKTLHPAIHAGILARSSEDDLQELHAHGYEPIDLVVVNLYP
                     FEETVRRHKMGKKALAEKRGKIKPSGPLADTKVPNIDEGMVSDAITINANTAPIANNT
                     MDAEAAEIVPSASLDESEIIEQIDIGGVALLRAAAKNHARVGALCDPLDYDLVACEIE
                     KGGLSSSTLRALAAKAFARTRDYDAAIAAWFLESNAESLNSDEFALPAAGLTSFGAEL
                     GNEEPGGVFSLVGRVERTLRYGENPHQKAFFVLPKTRSIGSIIRGPLGGQVLGGKELS
                     YNNLLDLDAAWRAVLDFEGPAAVIVKHLSPCGAAEADSLQEAYRAALACDPVSAFGSI
                     VALNRPLDLETALMMKGLFLECIAAPDFDEAAREILKAKKNLRLIAADPLLFEKEARE
                     LRSAAGGILVQEQDRGDPMNTKWSVVSDRKPTAAEWEAMRFAWKLVRHVRSNAIVLAA
                     GRAAVGIGGGQTNRVDAVRQACERAADRARGSVLASDAYFPFADGIEAAAGAGVTAVV
                     QPGGSVRDAEVLAAADKLGITMVYTGVRHFRH"
     gene            complement(3465..4208)
                     /gene="purN"
                     /locus_tag="PLQ30_04965"
     CDS             complement(3465..4208)
                     /gene="purN"
                     /locus_tag="PLQ30_04965"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPW01829.1"
                     /translation="MMTKNDGNRVSDSIVRIVVMASGEGSNLQALIDAIESGWIENMR
                     IEALLCDKPGAHCIERAICHGIPSHILPAPQKVKRSSPERRCYDERLAAVANSYKPDF
                     IFLLGWMRLLGRTFLENFPFRVINLHPALPGAFPGTNAIERAFEAFNQGRIANTGVML
                     HFVPDEGIDSGPVLSVVHVPINKGDTLSILEERIHSVEHRELINLARRLANNPRPKEI
                     QSQEMQETRDQDKLGEKESGHAYRIDIGI"
     gene            complement(4205..6610)
                     /gene="purD"
                     /locus_tag="PLQ30_04970"
     CDS             complement(4205..6610)
                     /gene="purD"
                     /locus_tag="PLQ30_04970"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPW01830.1"
                     /translation="MKVLLVGSGAREHALAIAIAASAECERLVISPGNAGMADVGEIA
                     NIGLQDIEGLCSLAQNIPADLVVIGPEAPLAAGLVDRLTEYGIACFGPSAGAAQIESS
                     KAFAKAFMVRHGIPTAEGKSFANPEEAKAWAKDFGRPLVVKASGLAAGKGVIVPENWK
                     ETEAAIERLAAEGEIVLEERLEGEELSLIALCDGKDYVVLPTARDHKRLLDEDKGPNT
                     GGMGAYSPACSMEDAERMACATIAPALSGLYEEGYPYIGALYAGLMLTAEGVKVLEYN
                     ARLGDPETQVILPLFDSDVLISLSACAHGRLGDARPRFKAEHAACVVLASLGYPDQPV
                     KGKPITIGDLPEKAYCLHAGTRLENGILVSSGGRVVSVLGIGSARAEALRAAYDAVSQ
                     VHFEGKQFRRDIGSKAERVEWAVKRALSAGTKESSVYAQVEESNPSSAYVRAGVDIDA
                     GNMAVELMKRAVRSTYNSRVIAGIGAFGGQYDALLVKDCKAPVLVASTDGVGTKTSLA
                     LKLDRLSHGKHKSGEPKDKRNNGSEENKGIWLTGLGADIVNHSINDILVQGARPLFFM
                     DYIAADRIDPAKVAKIVEGMSEACRQADCVLLGGETAEMPGTYREDEMDIAGTIVGIV
                     ERDKLLPRSDIAIGDVLIGLVSSGLHTNGYSLARSITSSMELERVYPELGESLASALL
                     RPHRSYLPLLAAILNANPCPIKALAHITGGGLIENIPRVLPPYLDAQIYAESWRWPAL
                     FLLLQRWGNVPDEEMRRVFNLGIGMVAIVEESQLNSFLNMLPEQAFVIGRLVSGRGNV
                     VLV"
     gene            complement(6607..8019)
                     /gene="purF"
                     /locus_tag="PLQ30_04975"
     CDS             complement(6607..8019)
                     /gene="purF"
                     /locus_tag="PLQ30_04975"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPW01831.1"
                     /translation="MIGQQEAKESCGVVAVVGEEAARLSFFGLFALQHRGQESAGIAT
                     LDEGNIYLHKDVGLVSQVFRDQNLAGLKGNMAIGHTRYSTTGRPSARNAQPLLIDTQY
                     GPLALGHNGNIANAPELRNQLLARGLGLMTGSDSELLAMMLAGTHGANWTERILSGMR
                     SWIGAFSLVILTHEGVFAARDPWGFRPLAWGRIASGGYAVASETSALRVMGCSELEEI
                     PPGMVLHLTEHGVVERGHADIQAQTAACSFEYVYFSRPDSVWNGKNIHAVRRKLGALL
                     ADEAPAEADLVIPVPDSSIAAAIGFSQRSGISFGEGLVKNRYIGRTFIEPTRALRQQG
                     VALKFSPLRETLEGARIVLVDDSIVRGTTTAQIIALCRRAGAREIHLRIASPRILYPC
                     YMGVDMGTKSDLMAVGRNLSEITKILGADSIAYLSIEGLSNAIGVDGLCKACFDGNYP
                     IPVNEGFQKECFECVSKGAL"
     gene            complement(8016..8543)
                     /locus_tag="PLQ30_04980"
     CDS             complement(8016..8543)
                     /locus_tag="PLQ30_04980"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPW01832.1"
                     /translation="MNRVRVIIFAGSPADKAHFETIQKALEVFGIPCLARIASAHKTP
                     KRLLDMIKSYDSEGIPTVYITVAGRSNALSGLVDAATQYPVLACPPPSEQWSAYDIWS
                     SLRMPSGIASALILDPVNAALCAAKLLALKDEALRENIRRFQQQQIDRLIEEDRLIEK
                     NETMEKKPMQERPRP"
     gene            complement(8545..9525)
                     /locus_tag="PLQ30_04985"
     CDS             complement(8545..9525)
                     /locus_tag="PLQ30_04985"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPW01833.1"
                     /translation="MVSEDLVRQLLPIAFRGTRKAEGEGKVREWWKLPSNKRLIATTD
                     RISAFDRVLAAVPFKGQVLNELSAFWFRKTEDIIANHLLSLPDPNCAVVKEVKPLPIE
                     VVVRGYITGVTSTALWRRYELGERIIYGQRFPEGIRKNEKLPHPVITPTTKGGPSGHD
                     ERLEPREVLEKGYLDAATWNDVQDAAFALFSTGTDCAERAGLILVDTKYEFGIAPDGS
                     LMLIDEVHTPDSSRFWLASSYQQRFEAGFEPESRDKEFVRLLYAELGYSGDGDIPKLP
                     DSVWIQTSILYQELYEKLTGLTFVPCSYPAEERILRTVEELKEKDAARRT"
     gene            complement(9538..10329)
                     /gene="purQ"
                     /locus_tag="PLQ30_04990"
     CDS             complement(9538..10329)
                     /gene="purQ"
                     /locus_tag="PLQ30_04990"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HPW01834.1"
                     /translation="MENRKPGVASMKAIILKAPGTNRDYDVQSAIEQAGGYADILPIS
                     RLRSDARLLQNYGMLVIPGGFSYGDALGAGRLFALDFLRFFAEEVSRYVESGRPVLGI
                     CNGFQVLVRAGILPGKQFEPNKFTLAHNESGSFECRWVTLLAPASNSIWTKGIDFVFC
                     PIAHGEGRFRSSSAKALDELEEKGCVALQYADSSGKLAHSNFPENPNGSMRDIAGICN
                     PEGNVLGLMPHPENAVFEWQTLALASPAANGARRLFENGLHYAAL"
     gene            complement(10296..13385)
                     /locus_tag="PLQ30_04995"
     CDS             complement(10296..13385)
                     /locus_tag="PLQ30_04995"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPW01835.1"
                     /translation="MANGENLYRFEVRTRGAGSPRIEALKADVRALGMGILRSARDTA
                     LYFVEGDLAPEELCLLGRFLLSDPVEETFEWREVSRQALHGSISEPDTVRIVEICRKP
                     GVTDTVANELVRAAREMGIPCLNRAATGIRWELEADWLNETSLLKLTRVLLANSLIDR
                     WEFGEINPIFPEGQLLVPPVEQYDLGIMDDNSLLSLSSERRLALDLTEMKAIQAWFVA
                     QRREITDVELEMIAQTWSEHCVHKSFKADIEVIRDSKPSDRESAFPRIIHSIFNTYIK
                     NTTEEIGAPWVKSAFVDNAGIVSFDDRWDLSFKVETHNHPSAIEPFGGANTGVGGVIR
                     DIMGVSAKPIAVTDILCFGPPDISEDELPQGTLHPRLVARGVVAGVEDYGNKMGIPTV
                     NGAVHFHPGYAANPLVYCGCIGLAPRGIHRNKPESGDHVIVLGGRTGRDGIRGATFSS
                     MKMDGSTGEVAGASVQIGDPIIQKKALDVLLAARDAGLYNAVTDCGAGGLSSAVGEMA
                     RELGAEIDLSGLGTKYPGLAPWELWLSEAQERMVLAIPPRHIKAFSELCSIFECEFWD
                     IGAFRSDGELKVRVKGELVLDLSMIFLHQGLPKKLLIAKEPLSKSPQERLQKSIEANV
                     YQARLDTLGIQTTEASNVHSELQGPDENRQASPGLIELKEQLLKLLGSREIASREKIV
                     RRYDHEVQGNSVIKPFVGAFCDSPSDAAVLKPKGAACEWGIALSNGFRSDYGELDPYR
                     GAWAAVDEAMRNIVASGGDPSRTAILDNFCMGDPNDPGVMWSLLESARGLREAALAFH
                     TPIISGKDSFYNEYLGADGSRHAVPPSLLISALAYVPKVERVVTTFFKHPADSIWLVG
                     DFKPSAIWQEEVPEISLRAQEVYSSLFDAIQEGEVLAAHDLSEGGLAVALAEMCIGGR
                     LGARIALSDIYEQRNIPISQTTETDNAILGVLRLALFGETMGCLLVEVGAGKEEQFAA
                     RFAPNSSKTSLQQNNLAKRIGEVLKEPSLEIVDPFLFMVSIGEMLDAWKTASQELLP"
     gene            complement(13606..14829)
                     /locus_tag="PLQ30_05000"
     CDS             complement(13606..14829)
                     /locus_tag="PLQ30_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPW01836.1"
                     /translation="MPSKKAWISLGATVVLILGGAVTPAHAFGSLVNWNILLIYLGSL
                     ILAELFIYSRVPAFIAQRIVERSPSIGIAITIILFLTGLLSAFVENVATVLVIAPIMI
                     ELAKRGKRNLAPLMIGLAVMANLQGTATLVGDPPSMLFANFAGYSFNDFFFKSGKPSI
                     FFAIQIGAIVGALYFYFYFRKTDSDIKVLPPEGIVAWFPSVLLLSMIGGLALFSAVSA
                     QGGIHPASGIFVFVLALVGLAWLVLAKKEKEEAKNLIIHLDWMTLVFLIGIFIVVGAL
                     AESSLLNQLAEQLAQWVKGDIFLGFALIVAISVLISGFVDNVPYIAAMLPVANALANA
                     MQIQPDLLMFGLLIGSCLGGNLTPFGASANIVAVGLSEKHGCKVSFWRWIKLAGPFTI
                     ITTIAASAFVWLVWA"
BASE COUNT         3740 a         4069 c         3317 g         3707 t
ORIGIN      
        1 aagcaatcgc aacatagcca aaggcttcga gcagcagaaa agactttcat gcctgcgctt
       61 cgggtgtttc atttgcagaa cagcttttaa ctgcgcaaaa agcgcataaa atccttttat
      121 ataagcatgg ttgcttcaat ctcgtttctg ataatttggt gcttctttgc taatgacgat
      181 gctatgtggg tggctttcgg cataagccgc ggctgtaatg cgtatcattc gcgccttcct
      241 tagttcggaa atagtccgag cgcccgcata tcccatgcct gagcgaaggc ctccgagcat
      301 ctgcaccata taatcagaga gctttccgcg atatggaacc ataccctcta caccttcggg
      361 aactgcctta ccactctgtc gaacgttcaa ctgcctatct gtcgatgcat cagactggcc
      421 agaaccgtaa cgatctcggc catatccttg gagagctccg atcgaaccca tgccacgata
      481 gcttttgaat tgacgacctt caaaaaacac aagttctcca ggggcttcct cgagacctgc
      541 aaggagattg ccaagcatga cggtctctgc gcctgcggct attgctttta cgatatcgcc
      601 tgaatagcga atacctccat ccgctatgat tgggatacca taggccttcg cggcttgtgc
      661 acactcatag attgcggaca actgaggcat acctacaccg gagataatcc ttgtcgtaca
      721 gattgagcca gccccgactc caacttttat cgccgaagct ccggcttgaa tcagcgcttg
      781 tgtgccctcg gcggttacta cattgccagc aattacatgg atatcgccag aaagagaccg
      841 aatgcgtcga attgcctcaa tcaccttttt cgtatggcca tgagcggtat cgatgacaag
      901 cacatctaca ttgcgagata gaagcaagct gactcgctcc tccaaatcag aaccaacgcc
      961 tacggcggca ccaacgagaa gtctgccacg cgcatccacc gctgcattcg gatactcttc
     1021 tttcttgacg atatctttta gggtgatgag ccctttgagc ctgccttctt cgtcgactaa
     1081 agggagtttt tcgattttgt aacgccgaaa aatcgcctta gcctcttcga tactagtccc
     1141 ttcgggagcg gttatgaggt ttttggaggt catgaattcc gatactggtt ttggcatatc
     1201 ctgtgggcca cagaaacgct tatcacgatt ggtgattatg cccactaagc gaccagtcaa
     1261 tggatcaaca acgggaattc cagagattcg gtaggtctcc attagatgct cagcttctgc
     1321 aagggtcgca gtttccggaa gccacacagg gtcggagatc atgcctgatt ccgaacgctt
     1381 gaccttgtac acctctgcag cctgagcttc tggcgatagg ttgcgatgga ctattccgat
     1441 gccgcctata cgagcaatgg ctatggctag cctggaatcg gtaacggtat ccatggccgc
     1501 ggaaagcacc ggagccttaa ggcgaattcc agggacaagc tccgcggaga tatcgacttc
     1561 tgaaggaaga acctcgctat atctaggcac gatcaagaca tcgtcgaatg taagacctgc
     1621 gccttcttgc agaagttttt caagactcat tttctacaac ctctcttaag atagttcttt
     1681 cgtgtccaat atggctctag tgccggaaat ggcgaactcc ggtataaacc atggtaattc
     1741 caagcttgtc ggccgcggcg agcacctcgg cgtctcgcac ggagccgccc ggttgaacca
     1801 ccgctgtcac gcccgccccc gccgcggcct caatgccatc ggcaaatggg aaatatgcgt
     1861 ccgaagctag taccgaaccc ctggcacggt cagcggcgcg ctcgcaagcc tgccgtactg
     1921 cgtcgaccct gtttgtctgt cccccaccta tacccaccgc cgccctgccc gccgccagca
     1981 ctattgcgtt agacctgacg tgcctcacca gcttccaggc gaatcgcatt gcctcccatt
     2041 cggctgcggt gggcttcctg tcgctaacca cactccattt tgtgttcatg gggtcgccgc
     2101 gatcctgttc ctgaaccaaa atgccgccag ccgcagacct gagctcgcgc gcctcttttt
     2161 caaataaaag aggatcagcg gctataagcc tgaggttctt tttcgctttt agaatttcac
     2221 gggcagcctc atcgaagtca ggcgctgcta tgcactccaa aaagagcccc ttcatcataa
     2281 gggcggtttc gagatccagg ggacggttca gagctacaat gctaccaaat gcactcactg
     2341 ggtcgcaggc aagggctgct ctgtatgctt cctgcagact atcggcctcg gcagcaccac
     2401 aaggcgagag atgcttgaca atcaccgcgg cagggccttc gaaatcgaga accgctcgcc
     2461 aggctgcatc tagatccagc agattattgt aggaaagctc tttgcctcct aatacctgcc
     2521 cacctaaagg cccgcggata atgctgccaa tcgatcgtgt cttgggcaga acaaaaaaag
     2581 ccttctgatg aggattttcg ccataacgca gcgtacgctc cactctgcct acaagcgaaa
     2641 aaacgccgcc cggctcttca ttgcccaact cagcgccaaa tgaggtcaat ccggcagccg
     2701 gcaaggcaaa ttcatcggaa tttaggcttt cagcattgct ctctaaaaac catgctgcaa
     2761 tcgcagcatc ataatctctg gttcgagcaa aggcctttgc tgcgagtgct ctcaaagtag
     2821 atgatgaaag acctcctttt tctatttcgc atgcaacaag gtcataatct aacggatcac
     2881 acaaggctcc tacccttgca tgattcttcg ccgcagcacg cagcagggcg acacctccga
     2941 tatcgatctg ttcaatgatt tcggattcat caagcgaggc gctcggcaca atctcagccg
     3001 cttcggcatc catagtatta ttagcaattg gagccgtatt tgcatttata gtaatagcat
     3061 cggaaaccat tccttcatca atatttggca cctttgtatc ggccaacggt ccagatggtt
     3121 ttatttttcc ccttttttca gcaagcgctt ttttacccat cttatgcctt cgcaccgtct
     3181 cttcgaaagg atagaggttc accactacga gatcgatggg ttcataacca tgagcatgca
     3241 attcctggag atcatcctct gatgatcgtg caagaatgcc ggcatggatt gctggatgga
     3301 gagtcttaac tcttccgccc aatagctccg gagaaccagt ataagccgcg atattcaatg
     3361 gttctagttt tgcctctctg agagttgcgg cagttcctcc tgaggcaaga aagcgccaac
     3421 cacaggctgc aagccgcttt gcaaaaggca caagtcctgt tttgtcatat accgatatca
     3481 atgcgatagg catgccctga ctccttttct cccaatttat cttgatctct tgtttcttgc
     3541 atctcttggc tttgaatctc tttaggcctt gggttattag caagccgccg tgcaagattt
     3601 atgagttctc gatgctcgac actgtgtatt cgttcttcca gaatgctcaa tgtgtcgcct
     3661 ttattgatag gcacatgtac aacactcagc accggcccgg aatcgatacc ctcatccggc
     3721 acaaaatgca gcatgacgcc tgtatttgcg attcttcctt gattaaaggc ttcaaaagcc
     3781 ctttctattg cgttggtacc ggggaatgcc cccggtaaag cggggtgaag gtttatgact
     3841 ctgaatggaa aattctccag aaaagtccgt cccaaaagtc gcatccatcc caacagaaaa
     3901 ataaaatcag gcttgtaaga atttgctact gccgcaagcc tctcatcata gcacctacgc
     3961 tcaggagaag aacgctttac cttttgcgga gccggcaaaa tatgcgaagg aataccatgg
     4021 caaatcgccc tttcgataca atgagcccct ggtttgtcac atagaagagc ctcaatacgc
     4081 atattctcta tccagcctga ttcgatagca tcgattagag cctgcaaatt gctcccttct
     4141 cccgaagcca tgacgacaat gcgcacaatg ctatctgaaa ctcgattacc atcgtttttg
     4201 gtcatcatac gagaaccaca ttccctctac ccgagactag tcttcctatc acaaaggctt
     4261 gttcaggcag catattcaag aaagagttga gctggctttc ttccactatc gcaaccatac
     4321 cgatccccag attgaagacc ctacgcatct cttcatctgg cacattcccc cagcgttgta
     4381 aaagcaaaaa tagcgctggc catcgccaac tctccgcata tatttgcgca tcgagataag
     4441 ggggaagcac acggggaatg ttctcgatca gcccgcctcc ggtgatatgt gccagagctt
     4501 taatcggaca tgggtttgca ttgagaattg ctgctaacaa aggaagatac gatcgatgag
     4561 gacgcaaaag tgccgatgca aggctttcgc ccagttccgg gtatactctt tcaagttcca
     4621 tcgaagaagt aatcgatcgt gccagggaat aaccattggt atgaagcccc gaagagacta
     4681 atccaatcag aacatcccct atcgctatat cggatcgggg aagaagctta tctctttcga
     4741 cgatccctac gattgtcccc gcgatgtcca tttcatcctc gcggtatgtc cctggcattt
     4801 cggctgtttc tcctcctagt agcacacaat ctgcttgtcg gcaagcttcg gacattcctt
     4861 cgacaatctt tgcgaccttt gccgggtcga tcctatccgc ggctatgtaa tccataaaga
     4921 agagaggacg ggcaccctgc accaggatgt cgttaatcga gtggttgact atgtcagcac
     4981 caagcccggt gagccaaatg cctttattct cttcggaccc gttattcctt ttgtctttag
     5041 gctcaccgga cttatgttta ccatgactca gtctatcgag cttaagagca aggctagtct
     5101 tggtacctac tccatcggta gaggcaacga gcactggcgc tttgcaatct tttacaagta
     5161 aagcatcata ttggcctccg aaggccccaa ttcctgcgat tactctggaa ttataggtcg
     5221 atcgtacggc tctcttcatt agctcaacag ccatattgcc ggcatcgata tcgacacccg
     5281 ctcgcacata agcgcttgat ggattgcttt cctcgacttg agcatagacg cttgattctt
     5341 ttgtccctgc cgaaagcgct cgctttacag cccactcgac cctttcggcc ttagagccga
     5401 tgtcgcggcg aaattgcttg ccttcgaaat ggacctgcga aacagcatca taagctgctc
     5461 taagcgcttc agcccgcgct gaacctattc ccaacaccga aaccacccta cctccggagc
     5521 ttacaagtat cccattttca agtcttgttc ccgcatgcaa acaataagcc ttctcgggca
     5581 gatctcctat cgttatgggc tttcctttta caggttgatc gggatatcct agagaggcta
     5641 aaactacaca agcggcatgt tctgccttaa atcgcggtct tgcatcaccc aatcttccat
     5701 gagcgcaagc cgacaacgaa atcaggacat cggaatcgaa aagcggtaga atcacctgag
     5761 tttcaggatc acctaaacgg gcattatatt ccagcacttt tactccttcg gccgtgagca
     5821 taagccctgc atatagtgct ccaatatatg gatatccctc ttcatagagt ccagaaagcg
     5881 caggagcgat tgttgcacaa gccattcttt cggcatcttc catagaacag gctggagaat
     5941 aggctcccat tcctccggta ttaggtcctt tatcttcatc cagaaggcgt ttatgatctc
     6001 tagccgtagg caataccaca tagtctttgc cgtcgcaaag agcgatcaga gacagctctt
     6061 caccttccag gcgctcttca aggacgattt ctccttcggc tgctaatcgc tctatggcgg
     6121 cctcggtctc tttccaattc tcgggtacaa tcacaccctt gccagcggcc aaaccagaag
     6181 ccttgaccac cagaggtcta ccaaaatcct tagcccaggc ttttgcttcc tctggatttg
     6241 cgaaactttt gccttccgcg gtaggaatgc catgcctcac cataaaagcc tttgcaaaag
     6301 ctttggagct ttcgatttgc gctgctccgg ctgatggccc aaagcaggca attccgtact
     6361 ctgttagccg atcgacaagg cctgcggcta gaggtgcctc tggcccaatc acaacaagat
     6421 cggcgggaat gttctgtgca agagaacaga gcccttcgat atcctgcaga ccgatattcg
     6481 ctatctctcc aacatccgcc atgcctgcat tgcctggtga aattaccagt ctttcacatt
     6541 ccgctgaagc cgctatcgct attgcgagcg catgttctct cgcccctgag ccgacgagca
     6601 gcactttcat aatgccccct tacttacaca ctcgaagcat tctttttgaa agccctcgtt
     6661 tactggtatg gggtaatttc catcgaaaca agctttacac aggccatcta cgccaatagc
     6721 atttgaaagc ccttctatac tgagatatgc aatggagtcg gctccaagaa tctttgttat
     6781 ctcagaaaga ttccttccta ccgccatgag gtcactcttg gtccccatat cgacacccat
     6841 atagcaagga tagagaattc tcggagaggc tatcctgaga tgtatctcgc gagctcccgc
     6901 tcttcggcag agtgcaatta tctgagccgt tgttgtaccg cggactattg aatcatcgac
     6961 tagcacaatc cgagctcctt caagggtttc tctgagcggg ctgaatttta gtgctactcc
     7021 ctgctgacgc aaggctcttg tgggctcgat gaaggtacgc ccaatatatc tgtttttaac
     7081 aagtccttcc ccaaaagaaa tacctgaccg ctgagaaaat cctatggctg cagcaatcga
     7141 agaatcaggc acaggaatga caaggtcggc ttctgctggt gcctcgtcag ccagtaaagc
     7201 accgagcttg cgacgaacag catgaatatt cttgccattc cacacagaat ctggccttga
     7261 aaagtaaaca tattcaaagc tgcatgctgc tgtttgggcc tgaatatcag catggccgcg
     7321 ctcgacaact ccatgctctg tgaggtggag taccataccc ggcggaatct cctcgagttc
     7381 cgaacagccc ataactctga gagccgaggt ttccgaagca acagcatagc cacccgaagc
     7441 tattctaccc caagcgagcg gcctaaaacc ccagggatcc cgggccgcaa agactccctc
     7501 atgcgtcaag ataacgagcg aaaatgcacc tatccaggat cgcattccac ttaggattct
     7561 ttctgtccaa tttgctccat gagttcccgc aagcatcatg gccagaagct cgctgtccga
     7621 gcctgtcatt agaccaagcc ctcttgcaag cagctgattc cttagctccg gagcattggc
     7681 tatattgccg ttatgcccta atgcgagagg cccgtactgt gtatcgatta gaagaggctg
     7741 ggcgttgcgg gcgcttggcc ttcctgtggt tgagtaacga gtatggccta ttgccatatt
     7801 gcccttaagg cctgcaagat tctggtctct gaagacctgt gatacaagac ccacatcttt
     7861 gtggagataa atgttcccct catcgagtgt ggcgatgcct gcagattcct ggccacgatg
     7921 ctgaagggca aagagaccaa aaaaggacaa tctcgccgct tcttctccca cgaccgccac
     7981 tacgccacag ctctccttgg cctcttgctg tcctatcatg ggcggggcct ctcctgcata
     8041 ggctttttct ccatagtctc gttcttttca atgagccgat cttcttcgat aagtcgatcg
     8101 atctgctgtt gctggaatct gcgaatgttt tcgcgcaatg cctcgtcttt cagagcgagc
     8161 agcttggcgg cgcagagagc cgcatttact ggatctagaa ttagagcgga agctatgcca
     8221 gaaggcatac gaaggctcga ccaaatatcg tatgcagacc actgctccga aggaggtgga
     8281 caagctagaa caggatactg agttgccgca tcgactaagc ccgataatgc attagatctg
     8341 cctgcaacag ttatatacac agtgggaatt ccctctgaat cgtaactctt tatcatatcg
     8401 agaagcctct ttggggtctt gtgcgcggat gcgatgcgtg ccaggcatgg aatcccaaat
     8461 acttcgagag ccttctggat tgtttcgaaa tgtgctttgt ctgcaggcga tcctgcaaaa
     8521 atgataactc tgacgcgatt catcttatgt cctccgagcg gcgtcctttt cttttagctc
     8581 ttccacggtt cgcagaatgc gctcttccgc agggtaagaa cacggcacaa aggtcaggcc
     8641 ggttagtttt tcatataatt cttgatacaa tatgctagtt tgaatccata ccgaatcagg
     8701 aagcttgggt atatcaccgt cgccactata gcccaactcc gcatacaaga ggcgaacgaa
     8761 ttccttgtct ctgctttctg gctcaaaccc tgcttcgaat cgttgctgat agctagaagc
     8821 aagccagaaa cgcgaagaat caggcgtgtg gacttcgtcg atcagcatga gcgaaccgtc
     8881 gggggcaatg ccgaattcat atttggtatc caccaggata agtcctgcgc gctccgcaca
     8941 atccgttcca gtagaaaaaa gagcaaaagc cgcatcctgg acatcattcc atgtggcagc
     9001 atccaggtag cctttttcga gaacttctcg aggttcgagc ctttcgtcgt ggccagaagg
     9061 gcctcctttt gttgtgggtg tgatgactgg atgcggaagc ttttcattct tgcgaatacc
     9121 ttcgggaaag cgctgcccat agattatgcg ttcccctagc tcataccgcc tccagagagc
     9181 agtggaagtg actccagtta tatagcccct gaccaccact tcgatcggga gcggcttgac
     9241 ttctttgacc acagcgcaat taggatcagg caaagaaagt aggtgattgg caatgatgtc
     9301 ctcagtcttt ctaaaccaga aggctgaaag ctcattgagt acctgcccct taaaaggcac
     9361 ggccgcaagt accctatcga aggcggagat tctgtctgtg gttgctataa gccttttgtt
     9421 gctgggcagt ttccaccatt ctctgacctt gccctctcct tcggcttttc ttgttcctcg
     9481 aaaagcgatt ggaagaagct gccttactag atcttccgaa accatgctgc ctccttttta
     9541 caaagccgca taatgcaatc cattttcaaa aagcctgcgt gcgccattcg cagcgggaga
     9601 ggccagggct agagtctgcc actcgaaaac cgcattctca ggatgaggca ttagtccgag
     9661 cacattgcct tccggattgc agatgcccgc aatgtctctc atagatccat tgggattctc
     9721 cgggaaatta ctatgggcaa gcttccccga agaatctgca tattgcagag cgacgcagcc
     9781 cttttcctcc agctcatcga gggccttggc cgagctggag cggaagcgtc cttcgccatg
     9841 agctatcggg cagaaaacaa aatctattcc ctttgtccaa atcgaattgg aagctggcgc
     9901 caaaagagta acccatctgc actcgaaact cccgctctca ttgtgcgcaa gagtaaattt
     9961 gttgggttca aattgtttgc ctggtaaaat tcccgctctc actaatacct gaaagccatt
    10021 gcaaatacct agcaccggcc tgccagattc tacatagcgg ctcacttctt ccgcaaagaa
    10081 gcgcaagaag tctagagcaa acagccttcc agcccctaat gcatcgccat aagaaaagcc
    10141 tccaggaatg accagcatac catagttctg tagtagcctt gcatcgctac gaaggcgaga
    10201 gatgggcaga atatctgcat agcctccagc ttgttctatt gccgactgaa catcataatc
    10261 tcgatttgtg cctggagcct tgagaataat ggctttcatg gaagcaactc ctggcttgcg
    10321 gttttccatg cgtcgagcat ttctcctata gaaaccataa aaagaaatgg atccacgatt
    10381 tcgagagaag gttccttcaa aacttcgcca atcctcttag ccagattatt ttgttgcaag
    10441 cttgtcttcg aagaattagg agcaaatcgc gccgcaaact gctcttcttt cccagcaccg
    10501 acctcgacca gaagacaacc catcgtctca ccaaataggg cgagccgtaa aacaccaaga
    10561 atagcattat cggtttccgt ggtctgactt atgggaatat tcctttgctc atagatatcg
    10621 gacagcgcga tccgagcgcc tagccttcct ccaatacaca tctccgcaag agctactgca
    10681 aggcctcctt ctgaaagatc gtgtgcagcg agcacttcac cttcttgaat cgcatcaaat
    10741 agggaagaat agacctcttg cgctcttagg ctgatttcag gcacttcctc ttgccaaatt
    10801 gcgctaggct taaagtctcc cacaagccag atcgaatcgg ctgggtgctt gaaaaaagtg
    10861 gtcactaccc gttccacctt gggcacataa gccaaagccg agattagtaa agatggcgga
    10921 accgcgtgcc tgcttccatc tgcgcccagg tattcgttgt agaaagagtc tttaccggag
    10981 ataattggag tatgaaaggc taaggcagcc tctcttaagc cccgtgcact ctccagaagc
    11041 gaccacatta ctccaggatc attcgggtct cccatgcaaa aattatcgag aattgctgtc
    11101 cggctcggat cgccgccact tgccactata ttccgcatgg cttcgtccac cgcagcccat
    11161 gcccctcgat atggatccag ttccccataa tccgacctaa aaccattgga aagcgctatt
    11221 ccccattcac aggctgcccc tttaggcttc agaaccgcgg cgtccgaagg cgaatcgcag
    11281 aaagctccca cgaagggctt gataacgcta ttgccttgca cttcatgatc ataacggcgc
    11341 actattttct cgcgcgatgc aatttctctg ctgccgagca gctttagaag ttgctctttg
    11401 agttcgatca gtcctggact cgcttgtctg ttctcatccg gaccctgcaa ttccgaatga
    11461 acattggagg cctcggtggt ctgtatgccc agggtatcaa gcctagcttg atacacattt
    11521 gcttctatcg atttttgtag ccgttcttgc ggcgattttg atagcggttc tttcgctata
    11581 agcagctttt tgggtaagcc ttgatgcaag aatatcatgc tcagatcgag tacaagctcg
    11641 cccttcaccc tcactttcag ttctccatcg cttctaaatg ccccgatatc ccagaattcg
    11701 cattcaaaaa tactgcatag ctccgagaaa gccttgatat gccgaggagg tatggctaat
    11761 accatgcgtt cttgagcctc ggaaagccag agctcccagg gggcaaggcc tggatatttg
    11821 gtgccaagac cagatagatc gatttccgcc ccgagctctc ttgccatttc accaacagca
    11881 gaagaaagcc ctcctgcccc gcagtccgta actgcgttgt atagtcctgc atcccgcgca
    11941 gccaggagca catccagagc ttttttttgg atgataggat ctcctatctg taccgatgcg
    12001 ccggcaacct ctccggtaga accatccatc ttcatcgaag aaaaagtggc tccgcgaatt
    12061 ccatctcggc ctgtacgccc acccaagact atcacatgat cgcctgactc tggtttattt
    12121 cgatgtatgc ctcgaggcgc aagtccaatg caaccacagt acacaagcgg atttgccgca
    12181 tagccaggat gaaaatgaac cgctccgttg acagttggaa tacccatctt gttgccataa
    12241 tcctcgacac ccgctacaac gcctcgcgct acgaggcgcg gatgaagcgt cccctgaggt
    12301 agttcgtctt cggatatatc gggaggtcca aagcaaagaa tatcggttac tgctatgggc
    12361 tttgcggaaa cacccataat atcacgaata acgcctccta caccggtatt agcgccccca
    12421 aatggttcaa ttgcagaagg atggttatgt gtttctactt tgaatgaaag gtcccatcgg
    12481 tcatcaaaac tcacaatacc ggcattatcg acaaatgcag actttaccca cggagctccg
    12541 atttcctcgg tggtattctt aatataagtg ttgaagatac tatgtataat tcttggaaac
    12601 gccgattctc tatcgcttgg tttggaatct cttattacct cgatgtcggc tttgaagctc
    12661 ttgtgtacgc aatgttcgct ccatgtctgg gcaatcattt caagctcaac atcggtaatc
    12721 tcgcgtcttt gtgcaacaaa ccaagcctgg atggccttca tctcggtaag atcgagtgcc
    12781 aatcgcctct cggaagatag agagagcaga gaattatcat ccatgattcc aagatcgtat
    12841 tgctcaaccg ggggaacaag aagctgtccc tctgggaaaa tgggattgat ttccccaaac
    12901 tcccatcgat caatcaacga attcgccaag agcactctgg tgagcttcaa tagtgaggtt
    12961 tcatttagcc aatctgcctc cagctcccaa cggatacctg tcgcagccct attgagacat
    13021 gggattccca tctctctcgc tgctctgaca agctcatttg cgaccgtatc agtaacgcct
    13081 ggttttcggc aaatctccac aatccggact gtatctggct ccgagatcga gccatgtaac
    13141 gcttgtcgcg acacctctcg ccattcaaaa gtctcctcta ccggatcgga taaaagaaaa
    13201 cgcccgagaa gacaaagctc ctcgggcgcc aaatcgcctt ccacaaagta cagtgccgtg
    13261 tcgcgcgcac tgcgcaatat ccccatccct agcgcgcgta catccgcttt caatgcttct
    13321 atgcgcgggc ttccggctcc tctggttcgg acctcgaacc tatagagatt ttctccgtta
    13381 gccatactac tatctagcgc gatatggata tattgtcaag ccgccaagaa atcttaggca
    13441 atggtggctt ttttaatttg gaccaataat cgctttataa tacttgccaa ttccggcctt
    13501 ttgaaattag cgaccaatag tcttttctgg aatattggta aacagccgat tttatcatgc
    13561 gcctttgacc caatatatgg caacctataa ccctgggaaa taacctcaag cccacacaag
    13621 ccagacaaag gccgatgccg caatagtcgt tataatagta aagggccctg cgagcttgat
    13681 ccatctccag aaactaacct tgcatccatg tttttccgaa agtcctactg caactatgtt
    13741 agccgaagct ccaaaaggtg taagatttcc accgagacaa gacccaataa ggaggccaaa
    13801 catcaacagg tcgggctgaa tctgcattgc atttgcaagc gcattcgcaa ccggaagcat
    13861 cgcggcaata tatggaacat tgtcaacaaa tcccgaaata agcacagata ttgctacaat
    13921 tagcgcaaaa cccaaaaaga tgtcaccctt aacccattga gccagctgtt ctgctagctg
    13981 atttaggaga ctcgattcag caagtgctcc caccacaatg aaaatgccaa tgagaaaaac
    14041 aagtgtcatc cagtctagat ggataatgag gttcttggct tcttccttct ctttttttgc
    14101 aaggacaagc catgccaacc ccacaagcgc aagcacaaag acgaatattc cactagcagg
    14161 gtggatccct ccttgagcag aaaccgcaga aaagagagcg aggcctccga tcatagacaa
    14221 aagaagcaca gatggaaacc aagcaacaat gccctctggc ggcaagactt tgatatcgct
    14281 atcagttttt ctgaaataga aataaaaata tagtgctccg actattgcac caatctggat
    14341 agcaaaaaag atcgatggct taccagattt gaaaaagaag tcgttgaatg agtagccagc
    14401 aaaattagca aagagcatag aaggaggatc gcccaccaaa gtcgctgtac cctgcagatt
    14461 agccattaca gcaagtccaa tcatgagagg agcaaggttt ctcttacccc gctttgccaa
    14521 ctcgatcatg ataggcgcaa taacgagtac cgtcgcaaca ttctcgacaa atgcggatag
    14581 cagacccgtc aaaaagagaa taatcgttat ggctatacct atcgaaggcg aacgttcaac
    14641 gattcgctgg gctataaaag caggaaccct cgaatagatg aaaagctcgg caagtattag
    14701 gctccccaaa tagattaaca gaatattcca gtttacaagc gaaccaaagg catgtgcagg
    14761 agtaaccgca cctcccaaga tgagcactac agtcgcgccc agagaaatcc atgccttttt
    14821 tgaaggcata aaa
//
LOCUS       DAOMZL010000044        14048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_3964_length_14048_cov_15.127993, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000044 DAOMZL010000000
VERSION     DAOMZL010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 14048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14048
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3964_length_14048_cov_15.127993"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..637
                     /locus_tag="PLQ30_05005"
     CDS             137..637
                     /locus_tag="PLQ30_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPW01837.1"
                     /translation="MPNDQTTTFLVDQRVVYPSQGVGRIMEIREKTFNNQKTLYYIIY
                     LEFSDMTVMVPVDKVEVLGIRPLVSRKEAEVALDSISKAASTIPSDWKLRYQMNLDLL
                     KKGSITDIAAVVRSLYQRSKIKELPILERKLYESALNLLQDEIAFALDRPKEEIAAII
                     RENLEK"
     gene            634..1869
                     /gene="ispF"
                     /locus_tag="PLQ30_05010"
     CDS             634..1869
                     /gene="ispF"
                     /locus_tag="PLQ30_05010"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPW01838.1"
                     /translation="MSSSVQKTISVHRLPIEGPSFGLITAAGSSSRYGSSKKELEDIG
                     GFTVLERAVEPFIPFCNAILITCPQGEKSEFEELFSKSKLKPDLASLQAGFLITEGGS
                     SRQESVLFGLRELAAHCNNDGVVLIHDGARPWISKKLIEKTLCCARQRGACLPLMPLT
                     ETPKVVEGGLIHDHPARGIIMCAQTPQAFSLMEILAAHELAHLEGFRATDDSMIWDHY
                     VGPVSWIEGERKNRKITFKEDLMSGLLAHESLSFPMPLRIGLGYDIHPLVDGRPLLLA
                     GVRIESEKGEAGYSDGDVLWHAIIDAMLGAAAAGDIGLLFPPGEPEWKDADSSVLARK
                     AFMLIRSKGWQLSNIDCTIIIEKPRLAPYREAIKASIAANLELSMESISLKAKTKEGF
                     DATGRGEAIEAHAVVMICR"
     gene            complement(1972..2772)
                     /locus_tag="PLQ30_05015"
     CDS             complement(1972..2772)
                     /locus_tag="PLQ30_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01839.1"
                     /translation="MNTITAYFAALFGNFLLSLGFSLQKKHVHWLSAKRRGESIRRGE
                     IIGWASGFVLMNVQPIFNYIALETLAPNIVAALGGSNIIFTIVLSYFLLGERMPPRKI
                     PFFVLLMASLVYAGVVGQEASRQFNVQAFWVAFFIPTAFSMLVLFASKRMSEDRIGLF
                     LGSSAGALGGFMVLALEALRINYGSNILSWFVSAYLYVYFFCGIASFFIKQFAFERGK
                     MTAVAPSFYGLLVLYPSIASYFVSNMPVHASQLIAFGGISVAIVLISI"
     gene            complement(2776..4011)
                     /locus_tag="PLQ30_05020"
     CDS             complement(2776..4011)
                     /locus_tag="PLQ30_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01840.1"
                     /translation="MISFENWKQGFVITIVGQRLLWHTRKRPAIFVHRSGEIRSAWRS
                     VGSCTPSMASKDSAFFIFSEGISMREEFQRYGEQGSVFLLEFSSIDSKIDAVKIRFLD
                     NSADEWNGLGPRPSHSLKGNINLKQGLHTAAFAIDGRFLLCESSGKRLFSFKDGAIEL
                     LLELPAAVYLGYAKNPIEACRLWHIWTMPDRHLARWIEVHKELEPQEDIGSLKPGNAA
                     GLLGSLSFSGARPNDFQELNLSIAAKAEGREDWKIADLMLCQPDIALPLLFRREIPMN
                     LELGNNRKRAQNRYAKIRAGLAQYWQFCEKRWTNEGIPALFHPSILFPDDCNNAESDN
                     MLFSGPDLVFAPNMASKDEFCSLILPKGSWIHLWTSRIYPAGKVTVYAPFGMPALFYR
                     LESEYAWLFDSVRQRASRL"
     gene            4254..5195
                     /locus_tag="PLQ30_05025"
     CDS             4254..5195
                     /locus_tag="PLQ30_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPW01841.1"
                     /translation="MKRFLVLGICLLMATSAFAQQQFITIATGGTAGTYYPLGGALAD
                     IWNKNISKMNATAQATGASIANINLLKGGNVDVIFTQNDVASYSYNGTELLKDQAYKD
                     LRGMACLYNETIQLVALESSGIKTIGDLKGKKVSVGAAGSGTEANARQILGAAGLTYN
                     DIKVQYLSFGESANNMKDGNIDAAFNTAGIPTAAIQDLAVSKKMVLVPIDGQIAAKLM
                     AQYPFYALQVVPAGTYQGQDQAVTTVAVKSMLAVSSKLSSDLVYKMLETMYANTDRLI
                     AAHSQGKNVKIESALEGMSIPLHAGAEKFFKEKGALK"
     gene            5322..5798
                     /locus_tag="PLQ30_05030"
     CDS             5322..5798
                     /locus_tag="PLQ30_05030"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HPW01842.1"
                     /translation="MLLGCLILIIALSAIAMNLRVVPVLIIKNGEDKVVARLPLHDGQ
                     FIHHYIHSIHKTAVDEYFHIKGRNLELFQVKYDTYGVGMPSDGGEAFAIEDGRFVVKM
                     HRTFFDIPILVSIVPQHGVIVEDELFLFTQWVLPESRITLSAGRMLIFYNGRSSPK"
     gene            5795..7789
                     /locus_tag="PLQ30_05035"
     CDS             5795..7789
                     /locus_tag="PLQ30_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HPW01843.1"
                     /translation="MSDLPIHEQETSTDAQEILKKFDKESNFRIYSGFFAKFISALAI
                     AFSVFQLYTAIFGVLDAMLQRSVHLSFGLALIYLLYPASKKWSFTKLHPLDALLAVAG
                     ALAPMYIIINYQKLVLRAGTATPMDIVFGIIGILLVLEAARRVVGIPMVVIAVVFIIY
                     AFIGPYIPGKLAHRGANFETLIQHLYFTTEGIFGIPLGVSSTFIFLFILFGAFLERTG
                     LGQLFIDLANSVAGWAAGGPAKVAVISSALLGTVSGSSVANVVGTGSFTIPMMKRLGY
                     KPEFAGAVEATASTGGQLMPPIMGAAAFLMAEFTGIPYSRIIGAAVVPAILYYFGVWA
                     GVHFEAKKNGLRGLSREELPKLKQVITERGHLIIPLIAIIYLLVSGYTPMRAALWAIV
                     LSIISSWIKKGTRIPPIEIVRALEAGARAALGVLAATACAGIIIGIVTLTGLGLKLGS
                     ILVDLAGGMLIPTLFFTMLTSLILGMGVPTTANYVITSTITAPAVIMLLSRKAGLDPY
                     AVAPANIILPAHMFAFYFGIIADVTPPVALAAFAGAGIAKANPMKTGINASKLAIAAF
                     LVPYIFVMNPQMLLFDVNFFSFVWMLITSLVGIISVASAVNGWLMTNTVWYERIIGFV
                     GGVLLIDPGLKTDLIGIGLIALLSISQFMRSKKLKAKASS"
     gene            complement(7898..10336)
                     /gene="thrA"
                     /locus_tag="PLQ30_05040"
     CDS             complement(7898..10336)
                     /gene="thrA"
                     /locus_tag="PLQ30_05040"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HPW01844.1"
                     /translation="MKILKFGGTSTGSPEALKSLVRIVRQSGEKILVVSALSGTTDAL
                     LSSAKLASEGGDWQSVFASIRERHYSMISALLQPPYKEIATGETRELLDELSSLLLGI
                     SLVRDLSPRTLDLVASYGERLSAGIIAETFSQTGLIAEAVDARKFIITDDKFGAAHWL
                     ESETIPRVESFFSDFEHIAVVTGFIASTIEGITTTLGRGGSDFTASILAACLEAEEIQ
                     IWTDVDGIMTADPRAVPDAFVIPEISYLEAMEMSHFGAKVLHPPAILPAMDRGIPVRI
                     LNTFNPDKPGTRIVRQGAPSSWPVRGIASIPSISLLLLQGPGLPGVAGIAARMFAALA
                     SAGINIILITQGSSELSICCAVLPQDSGKGVLALKEEFKYEIGAGTIQNPSIEQDLSI
                     IAVVGEMMKHRVGISGRVFKALGRNGINVVAIAQGSSELNISIAISVQDRNKAMCSIH
                     DAFFLAGVRTVNVFLVGTGLIGSTLLAQVAAQHRRLFRDRSIRLRIAGIANSRQMLID
                     PRGIDLENWKDALQSGKKTDISAFIQEILSLNLPNACFCDCTASEELAQHYEKLLSSS
                     ISVVTPNKRANSGPLREYQRLMDIAREMDVPYRYETTVGAGLPVIGTIQDLIASGDEI
                     LRIEAVLSGTMSFIFNNLGEKHSFSSLVAEAKLRGFTEPDPRDDLSAKDIVRKMIILA
                     REAGFPLEESQIQIESLLPKRIIDAPTVEEFLALLPEMDQMMKKCYDSASRSGAVLRY
                     VAIVTSRDARLSLSSFGPASPFYSLSGTDNMVVITSKRYSQNPLVIRGPGAGAEVTAG
                     GVFADILKTAESYL"
     gene            complement(10482..11612)
                     /locus_tag="PLQ30_05045"
     CDS             complement(10482..11612)
                     /locus_tag="PLQ30_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPW01845.1"
                     /translation="MEEFLSHFIASTGFIAITWGQIIMLIVSCVLLYLGIAREFEPLL
                     LVPIAFGMLLANLPMSGITDGPRDGLVGGLIYYLYEGVRLGIYPPLIFLGIGAMTDFG
                     PLIANPWSLLLGAAAQLGIFIPFIAAYYLGFDLKSCASIGIIGGADGPTAIYVTSRLK
                     PELLGPVAIAAYSYMALVPLIQPPIMKGLTTKKERQVKMAQLREVSKSERIVFPIFVT
                     ILIGLLLPAAVPLVGSLMLGNLFRETGVTERLSDTAQHALINILTIFLGVSVGSTASA
                     ERFLNPQTLMIIGLGLFAFSCGTAGGVFFGKIMYWVTRGKVNPLIGSAGVSAVPMAAR
                     VSQVVGSKEDPTNFLLMHAMGPNVAGVIGSAVAAGVFLSVLG"
     gene            complement(11630..12082)
                     /locus_tag="PLQ30_05050"
     CDS             complement(11630..12082)
                     /locus_tag="PLQ30_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HPW01846.1"
                     /translation="MKQYRITVNGKSYDVTVEELSGMRQSPSGAFGAGSASYSAPPAN
                     IQPTANVVSSANTAAPTASEQGSSNAAKAAPANNGSILTIKAPMPGTVISFKVIVGQK
                     VKRGDVLLLLEAMKMENEIVAPQDGTVVALRVPASAAVNTGDPLVDLA"
     gene            complement(12105..12476)
                     /locus_tag="PLQ30_05055"
     CDS             complement(12105..12476)
                     /locus_tag="PLQ30_05055"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HPW01847.1"
                     /translation="MTNPWMIMIVGVLTVFISLFIMIFTLLAFPSLFAAIKRREHKEL
                     APLPQIVEVSKKQAQPVGGQPVQEDDSLIAVLAAAVAEASGSRLDSFAITQVRQSPAE
                     SVEGFNTPIWGRVERLSRNER"
     gene            complement(12487..14040)
                     /locus_tag="PLQ30_05060"
     CDS             complement(12487..14040)
                     /locus_tag="PLQ30_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPW01848.1"
                     /translation="MVGMEEKLKELEERRAKVLAGGGPKRVAAQHAKGKMTARERIEM
                     LLDPGTFVELDAFVEHRATELGMDKIDAPGEGVVTGYGMINGRNVCVFAQDFTVIGGS
                     LGEMHAAKICKVMDLAVKIGCPLIGINDSGGARIQEGVDALSGYGEIFYRNTLASGVI
                     PQISVIMGPCAGGAVYSPALTDFVFMTEGSSNMFITGPQVIKAVTGEDVSPEELGGAR
                     IHSEVSGVAHFSLPDEQSTLEAIRKLLSYLPQNNIEDPPLIDIGDDPTRTDDSIADLM
                     PDSPNKPYDVRDIIIRLVDAGDFFEVQPGYAKNIVTGFARLAGRSIGIIANQPKHLAG
                     VLDINASDKAARFIRFCDAFNVPLLTLSDTAGYLPGVEQEHGGVIRHGAKLLYAYSEA
                     SVPKITVILRKAYGGAYIAMCSRHLAADQVFAWPTAEIAVMGPEGAANIIFKKEIEES
                     ADPEATRKEKIEEYKRSFANPYKAAARGYVDDVIDPAHTRMRLSNALNTLLGKRQSLP
                     SRKHGNIPV"
BASE COUNT         3832 a         3406 c         3155 g         3655 t
ORIGIN      
        1 gtcaggacaa aagattctca atctttaaag aggggttcga ttcccctagg ggctagaaaa
       61 tcctcgagca cagtcgaatg acgcctaata agggtgtttt gactatcatc cgtttttctg
      121 ctatcataga aaaactatgc ccaatgatca aactacaact tttctagtgg atcaacgtgt
      181 ggtctatcca agccagggtg tcggacgcat aatggagatt cgagaaaaga cctttaataa
      241 ccagaagact ctttattata ttatctatct tgaattttca gatatgactg ttatggttcc
      301 tgtcgataag gtggaagtac tgggaatacg cccgcttgtc tccagaaagg aggctgaggt
      361 ggcgctggat tcgatatcta aagccgcgtc cactatcccc tcggattgga aacttagata
      421 tcagatgaat ctagacttac ttaagaaggg ttcgataacg gacattgcag cagttgtacg
      481 ctcactctat cagcgctcca agattaaaga actccctatt cttgagcgga aattgtatga
      541 atcagcactc aatctactac aagatgaaat tgcctttgcg ctagatcgtc ccaaagaaga
      601 gatagccgcg attattcgcg agaatctgga aaaatgagct catctgttca aaaaaccatc
      661 agtgttcaca ggctacccat tgagggaccg tccttcgggc ttattacggc tgctggatcg
      721 agctcgcgtt atggaagctc taaaaaagaa ttggaagaca taggtgggtt taccgttttg
      781 gagcgagccg tcgagccatt tatcccattt tgtaatgcga ttctgatcac ttgcccacaa
      841 ggagaaaaat cagagttcga agagctattt tcaaaatcca aattaaagcc ggacttagca
      901 agccttcaag ctgggttctt gataaccgag ggtggttcaa gcagacaaga atctgtgctc
      961 tttggtctca gggaacttgc agctcattgt aataatgatg gtgttgttct gatccacgat
     1021 ggagctcgcc cgtggatttc taagaagctg atagagaaaa ccctttgctg cgcaaggcaa
     1081 agaggagctt gcctgccact tatgccgctg acagaaacgc caaaggttgt agaaggtggt
     1141 cttatacatg atcatcccgc aaggggaatc ataatgtgcg ctcagacacc acaggctttt
     1201 tctctcatgg agattcttgc tgcgcacgag ctagcacatc tagaaggttt tagggcaacg
     1261 gacgactcaa tgatctggga ccactatgtt ggtcctgtct cgtggataga gggagaacgc
     1321 aaaaaccgca aaatcacttt taaggaagat ctcatgtcag gtttgttggc ccatgaatcg
     1381 ctttcctttc caatgcctct gcgcatcgga ttaggatatg atattcatcc gcttgtagat
     1441 ggaagacctc ttctgcttgc aggtgttcga atcgaaagcg aaaaaggcga agctggatat
     1501 tctgatggtg atgtgctttg gcatgcaatt atcgatgcta tgctcggtgc ggcggccgca
     1561 ggagacatcg gtttgctttt tcctcctgga gagcctgagt ggaaggatgc ggattcttct
     1621 gttcttgctc gtaaggcctt catgcttatt cgcagcaaag gatggcaact atccaatata
     1681 gactgcacaa taataatcga aaaacccaga ctggctccat atagagaagc tataaaggcc
     1741 tcgattgccg ctaatcttga gctttcaatg gaatccatct ccttgaaagc aaaaacaaaa
     1801 gagggcttcg atgccactgg tcgcggtgag gcaatagaag cgcatgcggt agtaatgatc
     1861 tgtcgctagt tgtcgctgaa gcccacgaga gcctatggct atctttatat ctttatatct
     1921 ttgtatcttt gtatctttgt cgaatccttg tctttgcacc caataaatca cttatatcga
     1981 aattaagaca attgccacgc ttattccccc aaaagcgatg agttgtgagg catgaaccgg
     2041 catatttgac acaaaatagg atgcaataga aggataaagc acaagcaagc catagaaaga
     2101 aggtgccaca gcagtcatct tcccgcgctc gaaggcaaat tgcttgataa agaaagaagc
     2161 aattccgcag aaaaaataaa catagagata tgcggacaca aaccaactca atatatttga
     2221 accatagttt attctaagcg cttccagagc aagaaccatg aatcctccta aggctcctgc
     2281 agagcttcca agaaaaagtc caattctgtc ctcagacatt ctcttgcttg caaaaagaac
     2341 cagcatagaa aaagccgtag gaataaaaaa tgctacccaa aatgcctgta cattaaactg
     2401 ccgcgatgct tcttgcccga ccacacctgc gtatactagc gatgccataa gaagtacaaa
     2461 aaaaggaatt tttcgagggg gcattcgttc gccgagcaga aaataggaaa gcacaatcgt
     2521 aaagataatg ttcgaaccac ccaatgctgc tactatattc ggtgccaaag tctcgagagc
     2581 aatatagttg aagattggct ggacattcat caggacaaaa ccacttgccc aacctattat
     2641 ctcaccacgc cttatgcttt caccgcgacg cttagcagac aaccaatgca catgcttctt
     2701 ttgaagggaa aagcctagag acaagaggaa gtttccaaaa agtgcggcaa aatatgcggt
     2761 gatagtgttc ataatctata gtctgctggc tctctgcctg accgaatcaa aaagccacgc
     2821 atattcgctc tctagccggt aaaaaagtgc tggcatacca aatggagcat aaacggtaac
     2881 ctttcctgca ggatagatcc tggatgtcca caggtgtatc caacttccct taggcagaat
     2941 caacgagcag aattcatctt tgctcgccat attcggcgca aacacaagat caggccctga
     3001 aaagagcata ttatcgctct cggcattatt gcaatcatct ggaaatagaa tcgaaggatg
     3061 aaaaagggct ggtattcctt catttgtcca tcttttctcg cagaattgcc aatactgagc
     3121 aagaccagcc cgtatctttg cataacgatt ttgagcacgt ttcctattgt tcccgagttc
     3181 gaggttcatt ggaatttctc ttctaaacag aagaggaaga gcaatatcgg gctgacatag
     3241 cattagatcc gctatcttcc aatcctcgcg cccttctgct tttgctgcta tcgataaatt
     3301 caattcttga aagtcattgg gccttgctcc ggagaaagag agcgatccca gtaatcccgc
     3361 agcatttcca ggcttaaggc tacctatgtc ttcttgaggc tccaattcct tgtgtacctc
     3421 aatccatcgg gctaaatgtc tatccggcat tgtccagata tgccaaagcc tgcaagcttc
     3481 aatagggttt ttagcatagc ccagatacac ggcagcgggc aattctagca acaattctat
     3541 tgccccatcc ttaaaagaaa agagtcgctt tcccgacgat tcgcatagca gaaatctacc
     3601 atcgatagca aatgcagcag tgtgcaagcc ctgctttaga ttaatattac ccttcaagct
     3661 atgagatggc cgaggaccaa gcccattcca ttcatcggcc gaattatcta gaaagcgaat
     3721 cttaacagca tcgatctttg agtctatcga agaaaactcc agcaaaaaaa cggagccttg
     3781 ttctccatat cgctgaaatt cttctcgcat tgagatgccc tctgaaaaga taaagaatgc
     3841 gctatccttc gaagccatgg atggagtgca gctccctaca cttctccatg cagaacgtat
     3901 ttcgcctgat cgatgaacaa aaatggccgg ccgtttcctc gtatgccata gaagccgctg
     3961 gcctacaatg gttattacaa aaccttgctt ccagttctca aatgaaatca aaggtaatct
     4021 ccgatatctc tgcgatagcc ttctagccct caatcttggc cataatgccc gaataatcca
     4081 atgttattac tctatccaat ccaggcagga tgagcaacac cggtgagtca acacagtgag
     4141 caacatggtg agcaacacca tttaatattt gcattcgcga aaaacatagt gtataattct
     4201 aaaatcttag cttttatttc atgcccgatt ttttgtacag gaggtaaaga agcatgaaaa
     4261 gattcttagt actcggaatt tgtttgctca tggcaactag cgcatttgca cagcaacaat
     4321 tcattacaat tgcgactggt ggcactgcgg gcacttatta cccgctcggt ggtgctcttg
     4381 ccgacatatg gaataagaat atttccaaga tgaacgcaac agcccaggca accggcgctt
     4441 ccattgccaa catcaacctg ctcaaagggg gcaatgttga tgttattttc acacagaacg
     4501 atgtcgcaag ctattcttac aacggtacgg aattgcttaa agatcaagct tataaggatc
     4561 tgcgcggcat ggcttgccta tataacgaga caattcagct cgttgccctg gaatccagcg
     4621 gcattaagac cattggcgat ctaaaaggca aaaaagtgtc tgtgggcgcc gcagggtctg
     4681 gcacagaagc caatgctcgc caaattcttg gggctgcagg cctaacctac aatgacatca
     4741 aggtccagta tctgtctttt ggcgaatccg caaacaacat gaaggatggc aatatcgatg
     4801 ccgctttcaa tacggccgga attccaacgg cagccattca agatcttgcg gtttctaaaa
     4861 agatggtgct cgtgcctata gatggccaga tcgcagcaaa gctcatggca caatatccct
     4921 tctatgcgtt gcaggtagtc ccggcaggta cctaccaggg gcaagaccaa gctgtaacca
     4981 cagtagctgt aaaatcgatg ctcgccgtta gtagcaagct gagttcggac ttggtgtaca
     5041 aaatgcttga aaccatgtat gcaaataccg acagactaat tgcagcacat tctcagggaa
     5101 agaatgtcaa aatcgaatct gcccttgagg gaatgtcgat accactgcat gctggagcgg
     5161 aaaaattctt caaggaaaaa ggtgcgctga agtaagatta agatcatcgg agacgaacat
     5221 tagaaacgct atggacggcg cggaacctac tcgtttcgcg ccgtttatta agttattaag
     5281 acaagaacaa tatgaaacaa aggaggaaaa gaggcaagaa tttgcttttg gggtgtttga
     5341 tcttgatcat cgccctgagt gctatcgcta tgaatcttag ggtcgtgccg gtcttgatca
     5401 taaagaatgg cgaagataag gttgttgcac gattacctct ccatgatgga caatttatcc
     5461 atcattatat tcattcgatc cataagaccg cggtcgatga atacttccat ataaaaggaa
     5521 ggaatctcga gctttttcaa gtaaagtatg acacatatgg cgttggcatg ccttctgatg
     5581 gtggggaggc ttttgctatt gaagatggtc gttttgttgt caaaatgcac cgaacattct
     5641 tcgatatccc catcctggtg tcgatcgttc ctcaacatgg agtaatcgtt gaggacgaac
     5701 tttttctttt tacgcagtgg gtgctgccag aatcaagaat taccctctcc gcagggagaa
     5761 tgctgatatt ttacaatggg aggagttctc caaaatgagt gatttaccaa ttcatgagca
     5821 ggaaaccagc actgatgctc aagagatttt gaagaaattc gacaaagagt ctaattttag
     5881 gatatattcg ggtttctttg ctaaattcat ttcggcattg gcgattgcat tttccgtgtt
     5941 tcagctctat acagcgattt tcggagtctt agatgcgatg ctccagagat cggtccatct
     6001 ttcatttggc ctggcactta tctatctcct ttatcctgct agcaagaaat ggtccttcac
     6061 aaagctacat ccgctcgatg cattgcttgc ggtggcaggg gctcttgctc ctatgtacat
     6121 cataatcaac taccaaaagc tggtattgag agcaggaaca gccacgccga tggacatagt
     6181 ctttggcata ataggaatat tgctcgtgct agaagccgcg cgccgtgttg tcggtattcc
     6241 aatggtggta atcgcggttg tctttattat ttatgccttt attggcccat atatacccgg
     6301 caaactagca catagaggcg caaatttcga gactcttata caacatttat attttacgac
     6361 cgaaggcatt tttgggattc ccctaggagt ctcgagtaca tttatctttc ttttcattct
     6421 ttttggagca ttccttgaaa gaactggcct cggtcaactt ttcatcgacc ttgcaaattc
     6481 cgtagcaggc tgggcagctg gaggtccagc aaaagtggca gtcatatcaa gcgccctatt
     6541 gggtactgtt tcgggctcat cagtagcaaa tgtggtcgga acaggctcat tcactattcc
     6601 gatgatgaaa cgtctcggat acaaacctga gtttgccggc gctgtggaag ccacggcttc
     6661 tacaggcggc cagttaatgc caccaataat gggtgctgcg gctttcttga tggcggaatt
     6721 taccggtatc ccttattctc gaataattgg cgcagctgtt gtacctgcga ttctctacta
     6781 ttttggcgta tgggcaggtg tacatttcga agccaagaaa aatgggcttc gaggactgag
     6841 tcgagaagag ttgccaaaac tcaaacaggt cattacggaa agaggacacc tcataattcc
     6901 tttaatagca ataatctatt tgttagtatc gggatataca ccgatgagag ctgcgctatg
     6961 ggctatagtt ctttccataa tatcgtcatg gatcaagaaa ggtacgcgca ttcctccgat
     7021 agaaattgtg cgggcattgg aggctggtgc ccgtgcagct ctaggagtac tcgcggccac
     7081 cgcttgcgct ggtatcataa tcggcattgt aactctgacg ggccttggtc ttaaacttgg
     7141 atctattctg gtcgatcttg ctggaggaat gctcattcct acgctcttct ttacaatgct
     7201 cacatcgctt atccttggaa tgggagtgcc gactaccgca aactatgtca taacttcgac
     7261 tattaccgct cctgcagtca ttatgcttct ttctagaaaa gcaggcttag atccttatgc
     7321 tgtagcgccc gcgaatatta ttctgccagc ccacatgttt gcattttatt ttggtataat
     7381 tgcagacgtg acacctccag ttgctctggc ggcctttgca ggagcaggta ttgcaaaagc
     7441 aaatcccatg aaaacgggaa taaatgcatc aaagcttgcc attgccgcct tcctggtgcc
     7501 gtacatattt gtgatgaatc ctcagatgct cttgttcgat gtcaattttt tctcattcgt
     7561 ctggatgcta attacaagcc ttgtaggcat aatttcggtc gcatctgcgg tcaacggctg
     7621 gctcatgacg aatactgtat ggtacgaaag aataattggc tttgtaggcg gagtattatt
     7681 gatagatccg ggtcttaaga ctgacctcat aggaatcgga cttatagcct tgttgtccat
     7741 tagtcagttt atgcgctcga aaaaactgaa agcgaaagca tcgtcttgat tgcctcgata
     7801 gaacatcaat attgcggcga cgctaaagga atataagagc tttacagaag aggctgccac
     7861 atggcagcct ctttttgtat ttccttatcg agatctatca gaggtaagat tcggctgtct
     7921 tgagaatgtc ggcaaacact ccgccagccg taacctctgc tcctgcacct ggtccccgta
     7981 ttacgagcgg attttgagaa taacgctttg aagttataac caccatatta tcggttcctg
     8041 acagactata aaaagggctg gctggcccga acgaggagag tgaaaggcgc gcatctctag
     8101 atgttactat ggcaacatag cgcaagaccg ctccggatcg agatgctgaa tcgtagcatt
     8161 ttttcatcat ttgatccatc tctggcagca gcgccaagaa ttcttccaca gtaggggcat
     8221 ctattattcg ttttggaagg agagactcga tctgtatctg cgattcttcc aatggaaaac
     8281 cggcctcgcg tgccagaatt atcatttttc ggactatgtc ttttgcactc aagtcatcgc
     8341 gcggatctgg ctcggtaaat cctcgtagct tagcttccgc aacaagactt gaaaaagaat
     8401 gcttctcccc caggttattg aaaataaagc tcatggttcc agataacact gcttctattc
     8461 gaagaatttc gtctcccgat gctatcaggt cttgaatcgt gccgatgaca ggcagtccag
     8521 cccccaccgt tgtctcgtaa cgataaggca catccatctc gcgtgcaata tccatcagcc
     8581 tctgatattc gcgcaaagga ccgctatttg ctcgtttatt cggtgtcacg accgaaatcg
     8641 aagatgacag gagtttttca tagtgctgtg ccaattcctc gctagctgta caatcacaga
     8701 aacaggcatt cggtaaattc aggctgagta tctcttggat aaatgcagaa atatcggtct
     8761 tctttcccga ctgcaatgca tccttccagt tttctagatc aattcccctg ggatctatca
     8821 gcatttgccg cgaattagct atgcccgcta ttcgcaatcg aatcgaccga tctcggaaaa
     8881 gccttctatg ctgtgctgcc acctgtgcaa gcaatgtgct acctattagc cctgttccca
     8941 caagaaacac attgaccgta cgaactcccg caaggaaaaa agcatcgtgt atagaacaca
     9001 tcgccttatt tcgatcctga acacttattg ctatggagat attcagctct gaagatcctt
     9061 gagctatagc gacaacgttt atgccatttc tgcctagagc cttgaagaca cgccccgaga
     9121 ttccaactct gtgcttcatc atttcgccga caaccgcaat tatgctgaga tcttgctcga
     9181 tggaaggatt ctgaatggta cccgcaccga tctcatattt gaattcttct ttaagagcaa
     9241 gaactccttt ccccgaatcc tgaggaagca cagcgcagca gatggacagc tcactcgatc
     9301 cctgcgttat cagaatgata tttatgcctg cagaggcaag ggcagcaaac attctcgcag
     9361 caattccagc aacgcctggc aaacccggcc cctgtaacaa gagaagagat atggatggta
     9421 tggaggcaat accacgcaca ggccagctac ttggtgctcc ttgtcttaca atccttgtcc
     9481 ctggcttatc cgggttaaaa gtattgagta tccgaacggg aatacctcga tccatagcgg
     9541 gcagaatagc cggcggatgc aagacctttg caccaaagtg agacatctcc atggcttcga
     9601 gataggaaat ctccggtatg acaaacgcgt ccggaaccgc acgaggatcg gctgtcataa
     9661 ttccatcgac atcggtccat atctggattt cctcagcctc aaggcaggct gccaaaatgc
     9721 tagcagtaaa gtccgatccc cctcgaccca gggttgtcgt tattccttca atcgtcgagg
     9781 cgataaaccc tgttactaca gcaatatgct caaaatcaga aaaaaaggat tcgacacgtg
     9841 ggatcgtttc agattccagc caatgcgcag cgccaaactt atcgtctgtt attatgaact
     9901 ttcgagcatc cacagcctcg gcaatcaggc ctgtctgaga aaatgtctca gcaattatac
     9961 cagcactcag cctttccccg taggacgcaa ccaaatcgag agtccttggc gataagtctc
    10021 gtaccaatga aataccaaga agcaaagaag aaagctcatc cagtagctct cttgtctcac
    10081 ctgtggcgat ctctttataa gggggctgca gtagagccga aatcatcgaa taatgtcgct
    10141 ccctaatgga agcaaataca gattgccagt ctccaccttc gctagccaac tttgctgaag
    10201 aaagaagcgc atctgtggtg ccggaaagag ccgacacaac taggatcttt tctccagatt
    10261 gccgcacgat tcgcacgagc gacttcagtg cttcagggct gcctgtcgat gtaccaccaa
    10321 atttcagaat cttcatgatc cctcccattg gcggctacta tatcagaata aaacaatgag
    10381 cgaaaagact attacataac gaatgtatat aatattacct ggaaatatgg catcataatc
    10441 ctacgccata tttccttaat ccgaattgag ccagccaaac ctcagccaag caccgagaga
    10501 aagactccag cagcaaccgc ggaaccgatg actcctgcaa cattcggacc catagcgtgc
    10561 atgagcagga aattggttgg atcttcttta gaacccacca cctggctaac acgggcagcc
    10621 atcggcacgg ccgagacgcc tgcggatcct ataagaggat tcacctttcc acgggtgacc
    10681 cagtacataa tctttccaaa gaatactcct cccgctgttc cacaggaaaa agcaaaaagt
    10741 ccgagtccta ttatcatcaa tgtctggggg ttgagaaaac gctctgcact tgcggtcgag
    10801 ccaaccgaca ctcctaggaa aattgttaaa atattgatga gcgcgtgctg cgcggtatcg
    10861 gagagccttt ctgtcacgcc tgtctccctg aataggttgc caagcatcaa cgaacctacg
    10921 agagggactg cggcaggaag caaaagccct attagaatag tgacaaatat tggaaacact
    10981 atccgctctg atttggagac ttctcgaagc tgggccatct ttacttgccg ctcttttttc
    11041 gtcgtgagcc ctttcatgat aggaggctga atcagcggca caagcgccat gtacgagtat
    11101 gccgcgatag caacaggacc caaaagctcg ggcttcaaac gcgaagtaac atagattgcg
    11161 gtagggccat ctgcacctcc gatgattcca atactagcgc aggacttaag atcgaatccc
    11221 aaatagtaag ccgcaataaa gggtatgaaa atgcctaatt gagccgctgc acctagaaga
    11281 aggctccagg gatttgcgat tagaggccca aaatcggtca ttgcgcctat gccaagaaaa
    11341 attagcggcg gatatatccc aagccttacg ccttcgtaca aataatagat aaggccacca
    11401 actagaccgt cccttggccc atcggtaatg ccgctcatcg gaagattagc cagaagcatg
    11461 ccaaatgcaa taggcacgag gagaagaggc tcgaattctc gcgcaatgcc aagatataga
    11521 agcacgcacg atacaatgag cattatgatc tgcccccatg taattgcgat aaaacctgtc
    11581 gaagcaataa aatgagacaa gaactcttcc attgaggaat cctcctggtc tatgctagat
    11641 cgacgagcgg atcgcctgta tttactgcgg cgcttgcggg aacgcggaga gcaacgacag
    11701 taccatcctg tggagcaaca atttcattct ccattttcat ggcctctaat aggagaagaa
    11761 catcgccgcg ctttactttt tgtccaacaa ttactttgaa cgatataacg gttccaggca
    11821 tcggggcttt tatcgttaga attgagccat tgttcgccgg cgctgccttg gctgcattgg
    11881 aagaaccctg ttcagaggcg gtaggagctg cggtgtttgc ggatgagact acatttgccg
    11941 taggctgaat attcgctggt ggtgcggaat aagaagcaga gcctgcacca aatgcacctg
    12001 acggagactg ccgcattcca ctgagctcct cgactgtcac atcataagat ttgccattta
    12061 ctgtaattct atactgtttc ataatatatt tctcctcaaa tgttctagcg ttcattgcgg
    12121 gaaaggcgct ctactctgcc ccatatcggg gtattgaagc cttctacgct ctccgccggt
    12181 gactgacgca cctgcgtaat tgcaaaacta tcaagtctcg aaccactcgc ttcggctaca
    12241 gccgccgcaa gaacggcaat caatgaatcg tcttcttgca ctggttggcc gccaactggt
    12301 tgagcctgtt tctttgaaac ttcgacgatc tggggcaagg gtgccagttc cttatgttct
    12361 ctacgcttta ttgctgcaaa gagactggga aaagccagca aagtaaaaat catgatgaat
    12421 agcgatataa aaaccgtcag gactccgaca atcattatca tccatggatt tgtcatgcta
    12481 gacccctcaa acaggaatat tcccgtgttt gcgtgaaggc agactctgcc tctttccaag
    12541 cagcgtattc aatgcattgc ttagacgcat ccttgtatga gctggatcga tgacatcatc
    12601 gacatagcct cgcgctgctg ctttataagg gtttgcaaag ctgcgtttat attcctctat
    12661 cttctccttg cgggttgcct ccggatcagc ggactcttct atctctttct taaagatgat
    12721 attcgcagca ccctcgggac ccattaccgc aatttccgca gttggccagg caaagacttg
    12781 atcggcagcg agatgacggg aacacatagc gatataggct ccgccatatg ctttgcgcag
    12841 aatcacggtt atcttaggca cagatgcttc cgagtaagca tagaggagct ttgctccgtg
    12901 cctgatgaca ccgccatgct cctgctccac gcccggaaga taaccagcgg tatctgaaag
    12961 agtgagaagc ggtacattga atgcgtcgca gaaccggatg aagcgtgcgg ccttatcgga
    13021 cgcgttgata tcgagcacac ccgctagatg cttgggctga tttgctatta ttcctatgga
    13081 tcggccggct aggcgcgcaa agcctgtcac aatgttcttt gcgtatccag gctgaacctc
    13141 aaaaaagtcc ccagcgtcca caagtcgaat gataatgtct cggacatcat agggcttgtt
    13201 aggagaatcc ggcattaggt ctgcgattga atcatctgtc cgagtcggat cgtctccgat
    13261 atcgatcaag gggggatctt ctatgttgtt ttgaggcaga taggacagca gctttcttat
    13321 cgcctccaaa gtcgactgtt catccggaag gctgaaatgc gccacacccg agacttctga
    13381 gtgtattcta gcgcctccta gttcttcagg acttacgtct tcaccagtta ctgctttaat
    13441 tacctgaggg cctgttatga acatattgct cgatccctct gtcatgaaaa cgaaatccgt
    13501 caaagcgggc gaataaacag cccctccggc acaaggcccc ataataaccg aaatctgagg
    13561 aatgactcca gatgcaagcg tgttccgata aaaaatctca ccatagcccg atagagcatc
    13621 tacgccctct tgtattctgg cgccacctga gtcatttatt cctatcaatg gacaaccgat
    13681 ttttactgcg agatccatga ccttgcatat tttagcggca tgcatctcac caaggcttcc
    13741 acctatcacc gtgaaatcct gggcaaatac acatacattt cttccattga tcattccata
    13801 gcctgtaacg actccttcac ctggagcatc gatcttgtcc ataccaagtt cggtcgctcg
    13861 gtgttcgaca aaggcatcga gttccacgaa ggtgcctgga tctagcagca tttcgatcct
    13921 ctcgcgagca gtcatcttgc cttttgcatg ctgcgccgca accctcttag ggccaccacc
    13981 ggcaagcact ttcgctcggc gctcctctag ttccttcaac ttttcttcca taccgaccat
    14041 ccttgttg
//
LOCUS       DAOMZL010000045        13912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_4022_length_13912_cov_18.528109, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000045 DAOMZL010000000
VERSION     DAOMZL010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 13912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13912
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4022_length_13912_cov_18.528109"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /gene="prfA"
                     /locus_tag="PLQ30_05065"
     CDS             complement(<1..992)
                     /gene="prfA"
                     /locus_tag="PLQ30_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPW01849.1"
                     /translation="MEQLRGRLAEIDGLVSNSDILRDKNNYRNLMKERAKISEILQEF
                     DEVQKLNSSILETEELIRETKDEELRELAEQEILELKKQYALAEERLETLLAPRDPMA
                     DKSVIMEIRAGTGGEEAALFAADLYRMYSRYADGRGWNVEIMSSNETELGGIKEIIFS
                     ISGDAAYERLRLESGVHRVQRVPVTEGSGRIHTSTVTVAVLPEMEETEIEIRPEDLRI
                     DVMRSSGPGGQSVNTTDSAVRITHIPSGIVVHCQDEKSQIKNKAKAMRILRARLFEIE
                     DKKRQAERSAERRNQVGTGDRSERIRTYNFPQNRLTDHRIDLTLYKLDLIMEGDL"
     gene            1092..2372
                     /gene="serS"
                     /locus_tag="PLQ30_05070"
     CDS             1092..2372
                     /gene="serS"
                     /locus_tag="PLQ30_05070"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPW01850.1"
                     /translation="MLDIRFIKENLEAVRQNIRDRYMDADVDLLVSLYDKKNALLREL
                     EESRRLRNENAQAMKSNLSKEERAIRIEEGKRLKEIIAHLEAELQEIERAFAEELAKI
                     PNMAHPKAPRGKEDKDNLEVKRVGSPTQFNFPPKDHVELGQSLDILDFDTATKVSGTK
                     FYYLKNEGTILELALVRYALDMLSKHGFTLMTTPDIAKSQILEGIGFIPRGPESNVYT
                     LEGEEACLIGTAEITLGGYHSDSVLEKSQLPIRYAGVSHCFRREAGAAGQFSKGLYRV
                     HQFTKVEMFVYCLPEDSELIHEELRSIEEEIFSSLDIPFRVVDTCTGDLGAPAYRKWD
                     LEAWMPGRNNGEWGEITSTSNCTDYQARRLNVRYRDKDGKNRFVHMLNGTAIACSRAI
                     IAILENNQQADGSVMIPKALAPYCGFDLIRPNRT"
     gene            complement(2439..2918)
                     /locus_tag="PLQ30_05075"
     CDS             complement(2439..2918)
                     /locus_tag="PLQ30_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01851.1"
                     /translation="MNSEPNANKQPNNPPKHERPGKRIGSQHFRKHRSTRAGAQKTAD
                     RNQENHARMGEPSVESSRQESKICKVCGKPIFDLAGAIASREDGEPIHFDCAIEILSK
                     EENLAPDEKLIYIGSGFFAVCVQSSSDKLEIRRKIRWEISGTQHPWRKPMIFTPNLP"
     gene            complement(2915..3136)
                     /locus_tag="PLQ30_05080"
     CDS             complement(2915..3136)
                     /locus_tag="PLQ30_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01852.1"
                     /translation="MENNKVSRDIPAWAGSTPDDLLYSDYDITEEMKATNWKRLYEIK
                     HKLVDIGYLDGAIASIAEMVSNTIRNKPK"
     gene            complement(3126..3551)
                     /locus_tag="PLQ30_05085"
     CDS             complement(3126..3551)
                     /locus_tag="PLQ30_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPW01853.1"
                     /translation="MARIKYKNADLDTMLMSKAEGDKWKRKGREEEIMAGRYLESIGW
                     NVLARNFRSRRGEIDIVATRDNILSFIEVKSANQITERDLEHVIGAKKRNSIIETSKL
                     FLVINRKYKQYRIRYDVMLIQESACLRHIEGAFAEHDGE"
     gene            complement(3666..4037)
                     /gene="rplS"
                     /locus_tag="PLQ30_05090"
     CDS             complement(3666..4037)
                     /gene="rplS"
                     /locus_tag="PLQ30_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPW01854.1"
                     /translation="MDLIQQIQAEQIRTDLSDFRVGDTVRVSFKIVEGKSERIQAYEG
                     LVIAIKNAGVGKTFTVRKNSYGVGVERVFPFNSPRVEKVEIIKAGKVRRAKLYYIRSK
                     VGKKAKVKTLVGGRKSQKQEA"
     gene            complement(3994..4764)
                     /gene="trmD"
                     /locus_tag="PLQ30_05095"
     CDS             complement(3994..4764)
                     /gene="trmD"
                     /locus_tag="PLQ30_05095"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPW01855.1"
                     /translation="MRIKVISLFPEIIEAYFSASIMKRAVEKGLISYELVNIRDYAFD
                     RHRKCDDEVFGGGPGMLMKPEPLDRALAAAGSPEMRTIYVTPSGRLFNQVMALDFAHE
                     ENLILLCGRYEGIDQRIIDTRVTDLVSIGDYVLSSGEIAAMVVIDAVYRLIPGVISHE
                     SLSEESFSSGLLEYPQYTRPAKYGNLIVPEVLVNGNHAAIDRWRLKASLVKTLAYRPE
                     LLQNIDLTSELGRLLKEVIDEGGGHGPHTADSSGTNSN"
     gene            complement(4761..5288)
                     /gene="rimM"
                     /locus_tag="PLQ30_05100"
     CDS             complement(4761..5288)
                     /gene="rimM"
                     /locus_tag="PLQ30_05100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPW01856.1"
                     /translation="MHERQEPEYLAVARLGAVRGLTGELRLFSYSGEYSHIEDAQEFL
                     LGGREGLRDAKPIRVLRLQKASWGVSIVFEGFETPEKARRLVGRDIFLPRSKASPKKS
                     GEYYIADLVGMTAIADGEEIGVITAVIEGAESDLLEIERDFKKKILVPFRNEFVGKID
                     EERGELEIISPWILE"
     gene            complement(5304..5537)
                     /locus_tag="PLQ30_05105"
     CDS             complement(5304..5537)
                     /locus_tag="PLQ30_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPW01857.1"
                     /translation="MERDLVEYIVKSLVDHPESVDIKVVEGEKSTILELRVAESDIGK
                     VIGKNGRIARAIRTILSASASSKSKRVILEILD"
     gene            complement(5552..5803)
                     /gene="rpsP"
                     /locus_tag="PLQ30_05110"
     CDS             complement(5552..5803)
                     /gene="rpsP"
                     /locus_tag="PLQ30_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPW01858.1"
                     /translation="MSVRIRLKRFGAKARPYYRIVVMDSRSARDSKTIDEIGYYHPIE
                     AEDKQISINEAKVREWLAKGAQPSDTVRSLLNKKGIQLK"
     gene            6004..7953
                     /locus_tag="PLQ30_05115"
     CDS             6004..7953
                     /locus_tag="PLQ30_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPW01859.1"
                     /translation="MANTIPQMYIEIAQRQPNVPVQLSKDAHGDFQPVTYGELLREVS
                     IFALGLDDFGVRRGDNIGIISDNRKEWLIADLAILGIGAVDVPRGCDATAQEIAYILS
                     WSGCSTTILENERQLKKILDVKKGCLASNSMSLIETPYADLRSIILIDPPSSQIAQEA
                     QEAGLNIHLFSEIMEKGLSLYRNDPDFYQRETLKGTRADLATIIYTSGTTGEPKGVML
                     SHGNFLHQTEYFPYLLEVNSGDVFLSVLPIWHSFERAVQYILLQAGCTIAYSKPIGSI
                     LMEDMEVVQPHWFSTVPRIWESVKDGVDKTIRQSGGFSKFMFSMFVGIGKAHAYFKNM
                     LYDQLPKFSPRSRFLDISISIIPFALLSPFRALGNALVFKKIKGKLGKRFIAGVSGGG
                     ALPPSVDRFFEAIGILILEGYGLTETAPVLSVRLRNHPVTGTVGPVHRGTEVRILDER
                     GNVLPAGQKGVIHVRGPQVMLGYYKRPDLTEKVLSKDGWLNTGDLGMFTIHGELKIIG
                     RVKDTIVLRGGENIEPLPIEQRLRESPYIQQAAVVGQDQRYLAALIVPNQEVLTAWAE
                     ENDIPFNDYPSLLEQPEVKELINSEINSYISIKNGFKPFERIYYFALLPSPFEEGREL
                     SAKLDLKRHVINELYADQIKKLFEE"
     gene            complement(8036..8911)
                     /locus_tag="PLQ30_05120"
     CDS             complement(8036..8911)
                     /locus_tag="PLQ30_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPW01860.1"
                     /translation="MRTEGIDIDFRFIEDIQDVEGIRRIVIRVSEGNIIVTGSEDSQL
                     RYFIELQGVSEEIDTWSCRFRRAESIAIIEVIANDYVRIGKCNIFIPSHISDVEVHSS
                     RGSMEIREIPANVLAISDRGDISISNAKFVEVSSVNGNIIVDRSEGCSARSINGTIRC
                     SRIAGSVQVESQNSIVLMDQVEKNVAAVCDQGKIIARRVKGRVRLISNKGDIELEVSG
                     AFGGGEVQTYSGDISIQLEYSSIEFRAETLSGRIEANFPTSASGIGPQRCTFRTGEGS
                     RRLYVKSILGDIEIS"
     gene            9202..12381
                     /gene="ileS"
                     /locus_tag="PLQ30_05125"
     CDS             9202..12381
                     /gene="ileS"
                     /locus_tag="PLQ30_05125"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPW01861.1"
                     /translation="MYKPVDPKVNFSQQEESIVQFWQDQNIFQKSVSQREGAPEFSFY
                     DGPPFATGLPHFGHFVPGTIKDIFPRYKTMRGYHVERRFGWDCHGLPVENLIEKELGL
                     NSKTDIERYGIAEFNEACRGSVLRYVKEWRQIVTRLGRWVDFDHDYKTMEPDYMESIW
                     WVVKSLWDKGLIYEGHYILPYCPRCSTVLSNHELSLGGYEDVSDPSITVRFKITGLPP
                     SLDDPSLADLANGSTYFVAWTTTPWTLVSNLALCLGPDIDYVCVIDGSERYILADSKL
                     GDYYKNPESLKLSWKKKGSELVGIRYEPLFPYFAFLTEKGAFVTVLGQHVTTEEGTGI
                     VHTAPGFGEEDYEVLRGSGIPTVCPVDEECRFTEEVPDYKGLFVKDADKSIMNRLKNE
                     GKLIKRDQIIHAYPHCWRCESPLIYRAISSWFVEIEPIKKSMLKANKSVYWVPAHIRD
                     GRFGNWLENARDWAISRNRYWGNPLPIWRCDECGKTICVGSRKELAELGGEVPEDLHK
                     HYLDKVTLPCTCGGAMHRIPEVLDCWFESGAMPYGQNHYPFENKQKFESIFPADFICE
                     GLDQTRGWFYTLTILAAALFDKPAFKACVVNGLVLAEDGKKMSKSLRNYTDPSAVMDK
                     FGADALRLFLMNSAVIKAEDLCYSDEGVRNVLKSVILPLWNAYSFFVTYANIDGFVAE
                     DFDCAGVENPLDIWILSVCEKMIADVTTGFEAFDLQAAIAPTVEFVDSLNNWYIRRSR
                     RRFWKSENDADKKNAYKTLYRVLRRLTLVMAPVIPFVTEAIYRNIKLQSEPESIHLSD
                     WPNAEAKYRNLDLERDMRIVRQAVTMGRSLRVANDLKIRQPLGSIQLISRIAEEREAL
                     QRMEDILREELNVKSVVIHEKEEDLVEYHAKANFRVLGKELGPYMKEAAAKIANLSST
                     EIARLLDGEQILIALSSGEKIALDSSRVDIIREEKQGLKVLNEGSLTVALDTNITSEL
                     LYEGYVRDLIRGIQNARKEKGLDVTDRIILEVSGDDELKVALDRFGQIVAEETLALRI
                     DWKSMDEVMTNGFAVEAGDKAWMVNLRKA"
     gene            complement(12378..13634)
                     /locus_tag="PLQ30_05130"
     CDS             complement(12378..13634)
                     /locus_tag="PLQ30_05130"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HPW01862.1"
                     /translation="MSKVYSEFDFTRKNNRPENTFKLFLYACVVIFLIACSDNSQYLP
                     EGFAPFYPKFEAWENCDEGFALICVSSADGAQGFLEWRTEGVYGRIVETSGSRDTRSF
                     LIGDGGMNGLVLNARGKGDSLEISYEPTSQNAYIFQEGASQHRVTANRSKAIKDLKLL
                     AGLSEETQESGRKISMRLQYLVSRESLEEKAFNDFLRRGNSPYKMAEFRRQRLETSPI
                     AKYELPEDSGNLDSEPAKNVLKDDGSGQIRYWIPPIEFYEIQYPVYVGENIISVATQY
                     YLFNGGAHGSASTDFDIIDRRTGKRLGPSDIFVSDWRSTLGPKLKVELIRQSGFWQDS
                     AKSAKVGNDGNSPIEDLRDLGFFESDISPSDKIFLCKTGIGFEYDRYEIAPWYMGEFI
                     ILLSWDELRPFLNPALFADGQLLGSI"
BASE COUNT         3751 a         3388 c         2956 g         3817 t
ORIGIN      
        1 agatcacctt ccatgatgag gtcgagctta tatagagtca aatctatcct atggtcggtc
       61 aagcgattct gcggaaaatt ataggttctg atgcgctcgg agcggtctcc ggttccgact
      121 tgatttcgtc gttccgcgga acgctctgct tggcgtttct tatcttcgat ctcgaaaagc
      181 ctggctctga ggattctcat ggcctttgcc ttattcttta tctggctctt ctcatcttgg
      241 cagtgaacaa caatgccgct tggaatatgt gtaatgcgca cagcggagtc ggttgtattg
      301 acactttggc caccaggacc ggaggaccgc attacatcga tgcgcagatc ttctggcctt
      361 atctctatct ctgtctcttc catttcaggc aataccgcaa ccgtcaccgt gctggtatgg
      421 attctgcctg aaccttcggt taccggcaca cgctgaactc tgtggactcc ggattccagc
      481 cgtaacctct cgtatgctgc atctccagaa atcgagaaaa ttatctcttt aattccgcca
      541 agctcggttt cgtttgaact cattatctcg acattccacc cgcgaccatc ggcataacga
      601 ctatacatcc tatataggtc agcagcaaac agagccgcct cttctcctcc tgtgcctgcg
      661 cgtatctcca ttatgaccga tttgtcggcc ataggatctc gcggtgcaag aagagtctcg
      721 agccgttctt cggcaagagc atactgcttt ttcaattcaa gaatttcttg ctctgcaagc
      781 tctctcaatt cctcgtcctt tgtttctcga atcagctctt ccgtctccag gatcgaagag
      841 ttaagttttt gcacttcatc gaattcctga agaatctctg aaatctttgc tctctccttc
      901 ataagattgc gataattatt cttatcccta agtatatccg aattcgaaac gagtccatca
      961 atttctgcga gacgccctcg cagttgctcc atatgttcta tgtgttctat cacgaatgaa
     1021 tggtagcctg tttatggcct acacttcaac tcccgcttgc gccaagctgc tcatccggat
     1081 agtatctagc catgctagac atacgtttta tcaaagaaaa cttagaagcg gttcgccaga
     1141 atattcgcga ccgatatatg gatgctgatg tggacctatt ggtctccctt tacgataaga
     1201 agaatgcatt gctccgcgaa ctcgaggaat ccaggcgcct cagaaacgag aatgcccaag
     1261 ccatgaagtc gaatctatcc aaagaggaac gcgcaattcg catagaagaa ggcaaacgtc
     1321 tcaaagaaat aatcgctcac ctcgaagcag aacttcagga aatagagaga gccttcgccg
     1381 aagaattggc caaaataccc aatatggctc atcctaaggc tccccgaggc aaggaagata
     1441 aagataatct cgaagtgaaa agggtcggtt ctccaactca gttcaatttt cctcctaagg
     1501 atcatgtgga acttggccag agtctggata tactcgactt cgatacagca acgaaagtct
     1561 cggggaccaa attttattat ctgaaaaatg agggaaccat cctcgagctc gccctggtgc
     1621 gatatgcgct ggatatgcta tcaaaacacg gctttactct tatgacaacc ccagatattg
     1681 ccaagtcaca gattctggag ggaataggct tcattccgcg gggccccgaa tcgaatgttt
     1741 atactctcga aggagaagaa gcttgcctaa taggaacggc ggaaatcacg cttgggggtt
     1801 atcatagcga ttctgtcctt gaaaaaagcc aacttcctat ccgctatgcg ggcgtttctc
     1861 attgctttag gcgtgaagct ggtgccgccg gtcagttttc aaaaggcctc tacagagtgc
     1921 atcagttcac aaaggtagaa atgtttgtat actgcctacc agaggactcc gagttaatac
     1981 atgaagagct tcgctcgatc gaagaggaaa ttttttcgag cctcgatatt ccattcaggg
     2041 tcgtggacac ctgcacagga gatctcggtg ccccagccta tcgcaagtgg gacttggaag
     2101 cctggatgcc aggcaggaat aatggcgaat ggggagagat aacatcgacc tcaaattgta
     2161 ccgattatca ggctcgaaga ctcaatgttc gctataggga taaggatggc aaaaatcgct
     2221 tcgtgcatat gctgaacggc acagcaattg cctgctcccg cgccattatc gctattcttg
     2281 agaataacca gcaagcggat ggctctgtaa tgatcccgaa agccctagcg ccttactgtg
     2341 gtttcgatct tattcgtccc aatcgcactt gaggcatata tcttttaaga catctgtgga
     2401 aatggaacat caagaagcac ctagagcatc taaggaaact aaggcaaatt gggtgtaaag
     2461 atcatcggct tgcgccaggg gtgttgggtg ccagaaatct cccagcggat ctttcgtctg
     2521 atctccagtt tgtccgaaga agactgcaca caaaccgcga aaaagccgct cccaatatag
     2581 atcagcttct catccggcgc cagattctct tccttggaga ggatttcaat tgcgcaatca
     2641 aaatgaatgg gttcgccatc ttctcttgaa gcaatcgcgc ctgcaagatc aaatatgggc
     2701 ttaccgcaga ctttacatat cttggattct tgtcttgagc tctctaccga cggttccccc
     2761 atcctcgcat gattctcctg attgcggtca gctgtttttt gtgcaccggc gcgtgttgat
     2821 cgatgctttc taaaatgctg gctcccaatt cttttccccg gtctttcatg tttgggtgga
     2881 ttgttcggtt gcttgtttgc gtttggctcg gagttcattt cggtttattc cttatcgtat
     2941 ttgataccat ttctgcaatc gaagcaatcg ccccatccaa atagccgata tccactagct
     3001 tatgcttgat ctcataaagc ctcttccaat ttgtggcttt catctcttcg gttatatcat
     3061 agtctgaata taaaagatca tccggagtgg aaccagccca tgcaggaata tctctagaaa
     3121 ccttattatt ctccatcgtg ttccgcaaat gcgccttcta tatgcctaag acaggcactc
     3181 tcttgtatca gcataacatc ataacggatg cgatactgtt tatattttcg attaataacg
     3241 agaaatagtt tagaggtttc tattatagaa ttgcgctttt tggctcctat gacatgctcg
     3301 agatcgcgct cggtgatttg atttgccgat ttgacttcta taaaggacag aatattatcg
     3361 cgtgttgcta cgatgtcgat ttcccctcgc ctgctacgaa aattgcgcgc aagaacattc
     3421 cagccaatac tctcgagata tcggcctgcc ataatctcct cttctcttcc ttttcttttc
     3481 catttatcgc cttcagcctt agacataagc atcgtgtcca aatctgcatt cttatattta
     3541 atgcgcgcca aaatagattt ctcgattcaa aaatgttcgc gaaagcctag gattatataa
     3601 aaaagctgcc aaggcttctg cctgggcagc tcgatttcca aatcgctcta gagatcaaat
     3661 ggttcttatg cttcctgctt ctggctcttg cgacctccga caagagtctt gactttagcc
     3721 ttcttaccca ctttgctgcg aatatagtag agtttagctc tgcgcacttt accagctttt
     3781 attatctcca ctttttcgac gcgaggtgaa ttgaaaggaa acacgcgttc cacacctacg
     3841 ccatatgagt tttttcttac agtaaaggtc ttacctactc ccgcgttctt aatcgctatg
     3901 acaagccctt cataggcttg aattctctca gacttgcctt ctacaatctt gaaactgacg
     3961 cgaacggtat ctccgaccct gaaatcagac aggtcagttc gaatttgttc cgcttgaatc
     4021 tgctgtatga ggtccatgtc ctcccccttc gtctatcact tctttgagca atctgcctaa
     4081 ttcactggtc agatcgatat tctgcaaaag ttccggtctg tatgcgagcg ttttgacaag
     4141 actcgctttg agtcgccatc ggtctatcgc cgcatggttg ccgtttacca acacttccgg
     4201 gacaattaaa ttaccatatt tggcaggcct tgtatactgg ggatactcta gaaggccact
     4261 tgaaaaactc tcttccgaaa gagattcatg cgaaataaca ccaggtataa ggcgatacac
     4321 cgcatcgatc acaaccatcg ctgctatctc tccgctcgaa agcacataat ctcctatcga
     4381 aaccaaatcc gtaactcttg tatcgattat gcgctgatct attccctcat atcggccgca
     4441 gagtaaaata agattttcct catgtgcaaa atccaacgcc atcacctgat tgaaaagcct
     4501 gcctgacgga gtcacataga tcgtcctcat ttccggagat cccgccgccg caagagctct
     4561 atccaaaggc tctggcttca tcagcatgcc tggtccacca ccaaacactt catcgtcaca
     4621 tttgcggtga cggtcaaaag catagtctct tatattgact agctcatagg atatcagccc
     4681 tttttccacc gcgcgtttca ttatagaagc agagaaataa gcttcaataa tttctgggaa
     4741 aagagaaata accttgattc tcattccaga atccatgggc taatgatctc gagttcgccc
     4801 ctctcttcgt cgattttccc gacgaactca tttcggaagg gaaccaggat ctttttttta
     4861 aagtctctct caatttccaa gagatcgctc tctgctccct ctattaccgc ggtgatcacc
     4921 ccaatctctt ctccgtcggc tattgcggtc attccgacaa ggtcggcaat atagtattct
     4981 ccggattttt tgggactggc ctttgatcta ggcagaaaaa tatccctccc taccaatcgg
     5041 cgcgcttttt cgggcgtctc aaatccctcg aaaactatgc ttactcccca gcttgccttt
     5101 tgtagacgca aaactcttat gggcttggca tctcttagcc cttctctacc acccaagaga
     5161 aattcttgtg cgtcttctat atgcgaatat tctcccgaat agctgaatag cctgagctcg
     5221 cccgttagac ctcttacggc accaagtctt gccactgcca gatattcagg ttcctgcctt
     5281 tcatgcatga taaaaaatca atctcaatca agaatctcca agatcactcg ttttgacttg
     5341 gacgatgcgg atgcagaaag aatagtacga atcgcacgag ctattctgcc attcttgcct
     5401 atgacttttc ctatatcgct ctccgcaacc cttagctcga gaattgtgga tttctctccc
     5461 tcgactacct tgatatcgac ggattcagga tggtcaacaa gcgatttaac aatatattca
     5521 acaagatcgc gttccatagt gttctcacca ctcattttag ttgaatgcct tttttgttca
     5581 aaagagaccg aacggtgtcc gatggttggg caccttttgc aagccattct cttactttgg
     5641 cttcatttat gcttatctgc ttgtcctcag cttctattgg atgatagtat ccaatctcat
     5701 caatggtctt ggaatcgcga gccgagcggg aatccattac aacaatacga taatatggtc
     5761 tagccttagc gccaaacctt ttgagcctta tacgtacgct caccctaatc ctcctgaata
     5821 caaaaaatgc aatgtgggct tcgccttatc cgcacatccg ttcctctacg aagcccgata
     5881 tatggaacat gtgcggaaga tttatatact atgaatgggc aaagagtcaa cgcagtacct
     5941 tgaccatttt caattttaca tttatggtaa taatgtcaaa attgtcaaag gaggctcttg
     6001 gcaatggcaa atacaatccc tcagatgtat attgagatcg cacaaagaca gccaaatgtc
     6061 ccagtacagc tttccaagga tgcgcatggc gattttcaac cagtaaccta tggtgaactc
     6121 ctaagagaag tcagcatctt tgcgcttgga ttggatgatt ttggtgtcag gcgaggagac
     6181 aatataggta ttatttccga caatagaaaa gaatggctta ttgcggatct cgctattctc
     6241 ggaattggag cggtcgatgt tccacgcggc tgcgacgcaa cagcccagga aattgcatat
     6301 attctaagct ggagtggatg ttcaactaca attctcgaaa atgaacgcca actaaagaaa
     6361 atcctcgatg tcaaaaaagg ctgccttgct tccaactcaa tgtctctcat agaaacacct
     6421 tatgctgatt taaggtccat aattttgatc gatcctccat cgtcacaaat cgcccaggaa
     6481 gcccaagagg cagggctcaa tattcatcta ttctcggaaa ttatggaaaa aggcctatca
     6541 ctctatagaa atgatcctga tttctatcaa agagagactc taaaaggtac aagagccgat
     6601 cttgcaacca taatctacac ttctggtaca actggcgaac caaaaggtgt catgctcagt
     6661 catggcaatt ttctccatca aaccgaatac ttcccctatc tgctcgaagt gaatagtggc
     6721 gatgtgttcc tttcagtact tccaatttgg cattcatttg aacgagctgt tcagtacatt
     6781 ctcctccagg ccggatgtac gatcgcctat tccaagccca taggctcgat tctcatggaa
     6841 gatatggagg tagtacaacc ccactggttt tcaaccgttc ctcggatctg ggaatccgtg
     6901 aaagatgggg tggataagac aattcgccag agcgggggat tctccaaatt catgttctct
     6961 atgtttgtag gcattggaaa agcccatgca tatttcaaga atatgctgta cgaccaactg
     7021 ccaaaattct ctcccaggtc gcgctttctt gatatttcca tatctataat tccctttgca
     7081 ttgcttagtc cttttcgcgc cttgggcaat gcgctcgtgt tcaaaaaaat caaggggaaa
     7141 cttggaaagc gttttattgc aggcgtatct ggcggaggag ccttgccgcc ctcggtcgat
     7201 cgcttcttcg aagccatcgg aatattaata ctcgaaggat atggcctaac agaaaccgcg
     7261 ccggtattga gcgtgaggtt gagaaatcat ccggtgaccg gcacggtagg tccagtacat
     7321 cgaggaacag aagttcgcat tttagacgaa cggggaaatg tgcttcccgc agggcaaaag
     7381 ggcgttattc atgtgcgtgg tcctcaagtt atgcttgggt attacaaaag acccgatctg
     7441 accgaaaaag tactctctaa ggatggctgg ttgaacactg gcgatctagg catgttcact
     7501 attcatggtg agctaaagat aataggtcgt gtaaaagaca cgatagtatt gcgcggggga
     7561 gaaaatatcg agcctttacc catagaacag agattgcgcg agagccccta tattcagcaa
     7621 gccgctgtag tgggccaaga ccagagatat ctcgcagctc tcattgtacc aaatcaagag
     7681 gtgcttaccg catgggcgga ggagaacgat atcccgttta acgattatcc atcgttattg
     7741 gaacaaccag aagtaaaaga actcattaac agtgaaataa acagctatat cagcataaag
     7801 aatggtttta agccttttga gcgtatttac tacttcgctc ttctgccttc tcctttcgag
     7861 gaaggacgag aattgtcggc aaaattggat ctaaagcgcc atgtaatcaa cgagctctat
     7921 gctgatcaga taaaaaaact cttcgaggaa tgatcttata tcgacgagaa acctggtcaa
     7981 cccaatcaga aatttcgtgt caataatgcc gatgatagcc caaagaataa gaagctcaag
     8041 aaatctcgat gtcgccgaga atactcttga catagagacg tctggaccct tctcctgtgc
     8101 gaaaggtaca gcgctgaggt cctattccag aagcactagt ggggaagttt gcttctattc
     8161 gacccgaaag cgtttcagcc ctgaattcaa tgctgctata ctcaagctgt atggaaatgt
     8221 cacccgaata agtctggact tctcctccgc caaatgcacc gctgacttcc agttcgatat
     8281 cgcccttatt agagatgagc ctgactcttc ctttgactct gcgggcgatt attttgcctt
     8341 gatcgcatac cgccgctacg ttcttctcga cctgatccat caagacaata ctattctgac
     8401 tctccacctg gacagaacct gcaattctcg agcatctgat agtaccgttt atgctccggg
     8461 cggaacatcc ttcgctgcga tctacgatga tattgccatt gacgctcgag acttcgacaa
     8521 atttggcatt cgagatgctg atatctcctc gatcggagat ggcaaggaca tttgccggga
     8581 tttcgcgaat ctccatcgat cctctggagg aatgcacttc cacatctgag atatgtgaag
     8641 gtataaaaat attgcatttc ccaattctaa catagtcatt agctattact tcgataatcg
     8701 caatactctc ggctcgccga aaccggcaag accatgtgtc gatctcttcc gaaacccctt
     8761 gaagttctat aaaatatcgt agctgcgagt cttctgatcc ggtcacaatg atattgcctt
     8821 ctgacacacg gataactatc cgtcggatgc cttccacatc ttgaatgtct tctataaacc
     8881 ggaaatcaat atctatacct tcggtgcgca tcaaattatg cctccgggtt ctcaatccat
     8941 gcattacctg ccattctgta agatgcagat cttaatatta tttttattat tatatcacaa
     9001 tctctctttt tagcaaatcg atattagaat agtgtacgaa ttcccatctc gtgactccaa
     9061 cactagaggc cttttcgatt tcgcgatcct tttagcgatt ccttaccatt tttgatttcc
     9121 tttgtctttg catctatctt cctcctcttg aaaggatttg caacatacag cattattcgt
     9181 gacattaccg aggaggatat cgtgtacaaa cccgttgatc ccaaggtcaa cttctctcaa
     9241 caggaagaat cgatcgttca attttggcaa gatcagaata tattccagaa gtcagtttcg
     9301 cagcgtgaag gcgcaccgga attcagtttc tatgatggcc caccctttgc taccggactc
     9361 cctcactttg gccactttgt tccaggtacg atcaaagata tttttcctag atacaaaaca
     9421 atgcgaggct atcatgtaga aaggcgcttt ggatgggact gtcacggatt accagtagag
     9481 aacctgatcg aaaaagaact tggtctgaat tcaaagaccg acattgagcg ctatggcatt
     9541 gccgaattca atgaggcctg ccgaggctct gtccttcggt atgtaaaaga atggcgccag
     9601 atcgtaactc gcctcggccg atgggtcgat tttgatcatg actataaaac tatggaacct
     9661 gattatatgg agtcgatttg gtgggttgtc aaatcccttt gggacaaggg actcatatat
     9721 gaaggccatt atattctccc atattgtccg cgctgttcta cggtattatc caaccatgag
     9781 ctttcactag gcggatacga ggatgtcagt gacccctcca ttacagtgag gtttaagata
     9841 acgggtctac cgccatcgct cgatgatcct tctctcgcag acctggcgaa cggatctaca
     9901 tatttcgtcg catggacaac taccccgtgg acactggttt ctaatttagc tctttgtctt
     9961 ggcccagata tcgattatgt ctgtgtaatc gatggcagtg agcgatatat cctggctgac
    10021 tcgaaacttg gagactatta taagaatcct gaatcgctca agctttcatg gaagaagaaa
    10081 ggcagcgaac tagtcggaat tagatacgaa cctttattcc cttatttcgc cttcttgacc
    10141 gaaaaaggcg catttgtcac ggtgcttggc cagcacgtta ctaccgaaga aggaaccgga
    10201 atagttcata cagcaccggg ttttggtgaa gaggactacg aagtattgcg cggttccggt
    10261 attcctaccg tatgcccggt cgacgaagaa tgcagattta ccgaggaagt tccggactac
    10321 aaaggattat tcgtcaaaga cgcagacaaa tccataatga atcggctcaa aaacgaaggt
    10381 aaactcataa agcgcgacca gatcatccac gcatatccac attgctggcg ctgcgaaagc
    10441 ccgctcatct accgggcaat ttccagctgg ttcgttgaga tcgagcctat aaaaaaatcc
    10501 atgctaaagg ccaacaagag cgtctattgg gtgcctgccc atattcgcga tggtagattc
    10561 ggaaattggt tagaaaatgc gcgagactgg gcaataagtc gaaatcgcta ttggggaaac
    10621 ccgctcccca tctggagatg cgatgaatgc ggaaagacca tatgcgtggg ttctcgcaaa
    10681 gaacttgcgg aactcggcgg cgaagtacct gaagacctgc acaagcatta tctcgataaa
    10741 gttacactgc catgcacctg cggtggagcc atgcatcgaa ttcctgaagt cctcgattgc
    10801 tggttcgaat ccggagcgat gccatatgga cagaatcatt atcccttcga aaacaagcaa
    10861 aaattcgaat ccatcttccc tgcggacttc atctgcgagg gtctcgacca aacgcgaggc
    10921 tggttctata cattgacaat ccttgcggca gctctttttg acaagcctgc ttttaaggct
    10981 tgtgtcgtca atggtcttgt cttggcagaa gatggcaaaa agatgtcaaa aagcctacga
    11041 aattataccg acccctccgc agtcatggac aaatttggtg ccgatgcact gagactcttt
    11101 cttatgaatt ccgccgtaat aaaggccgaa gacctctgtt attcggacga aggtgtcaga
    11161 aacgtcctta agagcgtaat actcccactt tggaacgctt atagtttctt tgtcacatac
    11221 gcaaatatcg atggctttgt ggcggaagat ttcgactgcg cgggagtcga aaatcctctt
    11281 gacatatgga tactatctgt atgcgagaaa atgatagccg atgtaacaac cggcttcgag
    11341 gcctttgatc tacaagcagc aatagctcca actgtcgaat tcgtggatag ccttaacaat
    11401 tggtatattc gccgttcgcg gcgtagattc tggaaatcag aaaacgatgc agataaaaag
    11461 aatgcatata agacgctcta ccgggttctg cgccgactca ccctagttat ggcgcccgta
    11521 attccatttg tcaccgaggc aatctatcgc aacataaaac tgcagtcgga gcccgaatct
    11581 atccatctct cggattggcc aaatgcagaa gcaaaatatc gaaacctcga tctggagcgc
    11641 gatatgcgca tcgttcgaca agccgttact atggggcgct cgctcagagt agccaacgat
    11701 ctcaaaatcc gtcagcctct cggatcgatc caactcatca gcaggattgc ggaagaacga
    11761 gaagctctgc agcgaatgga agatattctt cgcgaagaac tcaatgtcaa atccgttgtc
    11821 atccatgaaa aagaagaaga tctcgtcgaa taccacgcta aagccaattt ccgggtgcta
    11881 ggcaaggagc taggccctta tatgaaagaa gcggcagcaa agatcgccaa tctgagcagc
    11941 acagagattg ctcgcctcct cgatggggag cagatattaa tcgccttgag ctcgggcgaa
    12001 aaaatagctc tggatagctc ccgagtagat ataatccgcg aggaaaagca aggcctaaag
    12061 gtcctaaacg aaggaagcct taccgtggcg ctcgatacga atatcacctc agaattgctc
    12121 tacgaaggtt atgtgcgcga tctgatccga ggaatccaga atgcccgcaa agaaaaaggc
    12181 ctcgatgtga ccgataggat tattctagag gtttcgggcg atgatgaact aaaggttgca
    12241 ctcgatcgct ttggtcaaat agtagctgaa gaaaccttag ccctaagaat agattggaaa
    12301 agcatggacg aagtcatgac aaatggcttt gcagtagaag cgggcgataa ggcttggatg
    12361 gtgaacctga gaaaagccta gatcgagccc aatagctgtc catcggcaaa aagtgctgga
    12421 ttaagaaaag gcctaagttc atcccatgaa agcaggatga tgaactctcc catataccag
    12481 ggagctatct cgtagcgatc gtattcaaat ccaattcctg tcttgcacag aaatatcttg
    12541 tcggatgggc ttatatcgga ttcgaaaaaa cctagatcgc gaagatcttc tatggggcta
    12601 tttccatcat tcccaacctt ggcagacttt gccgaatcct gccaaaagcc gctttgcctg
    12661 atcaactcca cctttagctt tggcccaaga gtcgatctcc agtcggatac gaaaatatca
    12721 ctgggtccaa gtcttttgcc agtccttcga tctattatat caaaatctgt cgaagcagaa
    12781 ccgtgtgctc ctccattgaa taaataatac tgcgtcgcaa cactaattat attctcaccg
    12841 acatatacag gatactgaat ctcatagaat tctatcggag gaatccaata tcgaatctgc
    12901 cctgaaccgt catccttaag gacatttttc gctggctcag aatccaaatt tccagaatct
    12961 tcgggtaatt catatttggc aataggagaa gtctctagcc gctgtcttct gaattcagcc
    13021 attttataag gagaattccc tcggcggaga aaatcattga atgccttttc ttcaagcgat
    13081 tcccgcgaaa cgagatattg tagacgcatg gaaatcttac gaccgctttc ttgagtctct
    13141 tcggacagac cggcgagcaa tttaagatcc ttaatggctt ttgatcgatt cgcagtaact
    13201 ctgtgttgac tagctccttc ctgaaatata taagcgtttt gagacgtagg ttcataggag
    13261 atctcaagcg agtctccttt tcccctggca ttcaatacca aaccattcat tcctccatct
    13321 cctatcaaga aagaacgagt atccctgctc ccactggtct ccacaatacg tccgtaaaca
    13381 ccttccgtcc tccattcaag aaaaccctgc gccccatccg cgctcgaaac acagatcaat
    13441 gcgaaacctt cgtcgcaatt ttcccatgcc tcgaattttg gatagaatgg ggcaaagccc
    13501 tccggcaagt attgagaatt atcgctgcaa gctatcagaa aaatgacaac acaggcatat
    13561 aaaaagagct tgaaagtgtt ctctggccta ttattcttgc gggtaaaatc gaattcgcta
    13621 tagactttac tcatataatc tgcaatccct gccgaaatta tattgtatag aagagattct
    13681 tacaaatttt atatcaaata tccctgctat gaaagccggg aagtgatata attaagattg
    13741 atttaatata ctcatataac ctagattaga tatgaaggag gcatctttta agaagaattc
    13801 ttgtttatca tgtttactca atcttgatat ggttatatca ttaaagaaaa gacctagata
    13861 tccgaggagg gtagtaatga aatttgtgaa aaggtttctc cctctgctgg ct
//
LOCUS       DAOMZL010000046        13701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_4108_length_13701_cov_13.257072, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000046 DAOMZL010000000
VERSION     DAOMZL010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 13701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13701
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4108_length_13701_cov_13.257072"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PLQ30_05135"
     CDS             complement(<1..308)
                     /locus_tag="PLQ30_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPW01863.1"
                     /translation="MDLKEILKPELISLDLKGRNKEEIIRELVDLASNSGKVLDKEET
                     IQSVLEREYRMSTGMKHGIAIPHGKTTAVHELVACVGITKEEVDFDALDRKGCRIFIM
                     T"
     gene            complement(374..1615)
                     /locus_tag="PLQ30_05140"
     CDS             complement(374..1615)
                     /locus_tag="PLQ30_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HPW01864.1"
                     /translation="MLPKMKLWHKYALGIAMGAILYLVLPPSMIHGTPMISFLSELAL
                     KIGGYALILTLTVGIPISVFRLSEAHRFTKIVSQSLLLSVVSLVVAAGIGIAVVLVFK
                     PVPLPLVSQSGTVQTLDPLELILAIFPTSIFDVFSVSAKWFLPIGLFALFFGLALSHD
                     PVMSRPILPVLDVISRASYLINFFISEILGILLIPISLYLFLNLRNTGIPSEYRSVLL
                     FVLILTVFLIFVLFPLVYRILGGKSNPYILLYAMSGPLLTAAASGSIFISSATALRHV
                     SESLGIKRDTNAVVFPLVLVGGRVGSVIIVACSFIAMFLSYSRNNPTAGQIILLLALL
                     PLSVTVGASALRSDIIVMLSLVCVLFGQGFQNGASLLVPIALPLSICATILDTAWLMF
                     SLSIIAELNGQRSVKKARNFI"
     gene            1735..1808
                     /locus_tag="PLQ30_05145"
     tRNA            1735..1808
                     /locus_tag="PLQ30_05145"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1769..1771,aa:Arg,seq:cct)
     gene            2009..2482
                     /locus_tag="PLQ30_05150"
     CDS             2009..2482
                     /locus_tag="PLQ30_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HPW01865.1"
                     /translation="MHRLERGNQELEEDVELLKIAGLLAAKKVRRFRYLVQQKYRYRI
                     KKACKTLGISHSGFYAYRARKPSDRQLENEFLSHKIHEVFEQHSGRYGAKRITYALKD
                     EGIKVNRKRIVRFMLAMGLYTKGAKRAYKHYNKHHRTVAKAIPTTMRSWNRSIRR"
     gene            complement(2758..3453)
                     /locus_tag="PLQ30_05155"
     CDS             complement(2758..3453)
                     /locus_tag="PLQ30_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPW01866.1"
                     /translation="MKKFGKFLLILTVILFICIVPSAFAQNLGGEAGIGFMAISPPLT
                     DTLSSFNVQDMLYGVTVSYVVRPWLGVSTDVMYLGDLYYGPGGGAFTEGPLSWTSLSS
                     KTGSKADWKYYESFIYAPLSINLIAPLRSVRPYLGLGPAFYFHFASTNQDAAFTDYLK
                     SYYGSSGLNSRIGIGLTMRVGLDLLLSDSFGFGLGYVVREDTPATIFDDLGRLDFYKE
                     KGYLFVLGKFYIK"
     gene            3585..3953
                     /locus_tag="PLQ30_05160"
     CDS             3585..3953
                     /locus_tag="PLQ30_05160"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPW01867.1"
                     /translation="MKTTFSEKAAFMWQDEFGAAITVIDRDYRILYMNDKSIEANATR
                     GGKALLGHDVRACHQERSVRIIEHILETGEPNVYTIEKQGVHKLIYQAPWRHNGVIAG
                     VVELSIEIPATMPHYVRDVQ"
     gene            3969..4202
                     /locus_tag="PLQ30_05165"
     CDS             3969..4202
                     /locus_tag="PLQ30_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPW01868.1"
                     /translation="MLETAKALFALEGMNVSMNDIARKIGIPVGSIYTYFPSKQALIE
                     TIIEEGWNQFRTWLEQQLAMIEDNLDDCAIGSA"
     gene            complement(4253..5281)
                     /locus_tag="PLQ30_05170"
     CDS             complement(4253..5281)
                     /locus_tag="PLQ30_05170"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW01869.1"
                     /translation="MRVLVTGGLGNVGSRAVRALLAMGYEVVIFEHPSARQKRTRQSR
                     ALLGSAKGKLTIFYGDVSDAATMSALGDFLQSKDVRAVLHLAGIIPPLATRAPELAQR
                     INVEGTQNVIALCKHLPKPPRIVFASSIALYGDRLANPWISAQDPLCPNDAYALTKQQ
                     CEAMLQKGDLPWTILRLSYVVASDWLPLDPLLFEVPLATHFEVVHTEDAGRAFAAAWS
                     TLATEGRILNIGGGILCRTTYRAYLDRMMRAFGLGDSSFLSDDLFATRNFHCGWLTDS
                     DEAELLLNFRSKTLEDYYSEVAWRERVIAPFAKVGAPFAKRWLRSLSPYRAKAGLARL
                     ERSVLEKP"
     gene            5552..6664
                     /locus_tag="PLQ30_05175"
     CDS             5552..6664
                     /locus_tag="PLQ30_05175"
                     /inference="COORDINATES: protein motif:HMM:NF015754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCCL domain-containing protein"
                     /protein_id="HPW01870.1"
                     /translation="MHGKQNLTRSFVLIICAMGVFVLMPAFGQSSPNLRGIAGLQSGS
                     VLFLSIKGDVSGASSLWGTDIYTLDSNVAAAAVHAGVLANGQTGIVAVVICDGLSSYS
                     GSFRNGVQSNSWGSFDLSFRFVRLAQAPGSLAQATPSAPPATQAPPTQPAKPPVASRN
                     LYVFDDPNVLKGNGNFKPGTVIYVQVTGRAAGAKLWGTDIYTSDSDLTGAVVHTGILS
                     DGQTGIVKVTILPGQQSYVGSNRHNVVSLSFGSWAMSYSLEPVTQSYDRVVRMILDPG
                     SADHIPNVTTGNSYVVWVTGSADQRSVWGTDIYTSDSPFATAAVHAGILKVGESGPVI
                     VHVLEGQSHYEGSTRNGVTTKSFGSYGRSYSLEPAR"
     gene            7391..8170
                     /locus_tag="PLQ30_05180"
     CDS             7391..8170
                     /locus_tag="PLQ30_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPW01871.1"
                     /translation="MAEVNSVLKAFAILDLLSDGKKRSLSDIAHELSLPKSTVSNLLE
                     TLTSERVLERDYGSGLFSLGIRLIELGYCTQTGNDLVRIAAPFLRGLNQRFDETVHLV
                     LLDRDEVLYIDCIESKRRLRTYSVIGIHAPLHCTSVGKAILAFLPNAEIQRILKNKGL
                     PSFTPHTITTEDRLWREIAVIRERGYAIDDMEHEDHLRCVGAPIFNSKGEVFASVSIS
                     GPAERNTLKRLELMTPALLEATAEISHRLGYRNKDHSRASS"
     gene            8200..9132
                     /gene="dapA"
                     /locus_tag="PLQ30_05185"
     CDS             8200..9132
                     /gene="dapA"
                     /locus_tag="PLQ30_05185"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPW01872.1"
                     /translation="MAKFVKEYYGKILIPMLTPFREDGSVDYEKAAALAEFIINQKKG
                     DSLVLAGSTGEFYAMRFDERVRLFETIKERVGNRIPIIPGVGAASTIETIQLAKKAEE
                     IGFETVMIVAPYYTKPNQEELYRHFMQVATSVPVNILLYNIPIYTGVNIEPATLCRLA
                     KQPNIVGIKEEAELNPKQITAFLNATPEEFVVYNGDDTMILEAYAQGGEKRIGGVVSG
                     SSHIIGHLIRSMIEDFLAGKVQAAAEQQRKIYPFLKCLSQNNRTNGAPLLKEALRLYG
                     FDAGYPRAPLIQGSPEEIENIRIAMVAVGVLKAG"
     gene            9184..10182
                     /locus_tag="PLQ30_05190"
     CDS             9184..10182
                     /locus_tag="PLQ30_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid TRAP transporter substrate-binding
                     protein SiaP"
                     /protein_id="HPW01873.1"
                     /translation="MKKCIVAVLIMLLVASTAVLAQQKPVKLVFTSVSVPTDAHTQAM
                     YVFKDEVEKLSGGQIQVDVYDSGKLFTQQAEQDAIRKGTVDMVYSSASWLAEFVPYLS
                     MFGAAYTFQSYDQMTKTFNGPIGKKIFEDVAKKTGVRPLCAFYLGTRQLNLIAKVGPV
                     TKPEQMKGVKLRVPQSPTWIAMGKALGANPTPMAFNELYMGLKTGAVDGQDNPLPTDK
                     NAKFYEVTKYIVLTNHLVDSVWPSINEKKWQSLTKQQQDWIMQALEKARQFCDKTNLD
                     NEKEILDFFRQQGLIVIENPDRDAFAAYAKNFYLTEGKDISKDWDMALYDEIQKLK"
     gene            10320..10817
                     /locus_tag="PLQ30_05195"
     CDS             10320..10817
                     /locus_tag="PLQ30_05195"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPW01874.1"
                     /translation="MPDWLKKTGKFLVDCIELYVPILTFSTLFVSFVIQIFARKFFKP
                     LIWPEELSLICFVWTALLGGLYSKRMGTQVAFTMLYDAAKPETKRIMRIVGNALLLIA
                     FAIILVPSWNYVQFMAYKKSDALRIPMNIAYFPFVVYLVDMIVRLALEIVKEFTTPEA
                     PGGAE"
     gene            10814..12112
                     /locus_tag="PLQ30_05200"
     CDS             10814..12112
                     /locus_tag="PLQ30_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPW01875.1"
                     /translation="MNIPLIVFVVSFVAVFLLGIPLAPGMMLSSAFYFLLSDNPAASL
                     DMVAIQFLTNLNASFVLIAVPLFVFMAEIMNSGKVTEMIFSFSSALVGKRKGALAHVN
                     IINSIIFSGMTGSALADASGPGNMELKAMQQQGYDDAYSCAISAASAIIGPIIPPSIP
                     MVMYAMLSSTSIGYLFLGGIIPGLLIGIALLIYNAIISNKRNYPEGKSFPPREFWKFT
                     LQALPALFTVVILLGGIYTGVVTPTEAGALAAFYGILISVFVYRALGWKELKQCLINA
                     VRTTGTLSLLVGSSFAFSYIVSIEQIPNVIATFLLGLTQNKYVMLFILNIALLILGCF
                     LDTTPIQLIFTPIIIPILSQLGIDLVHFGVVMVLNLMIGLATPPYGMLLFTVSGLSGA
                     PLKDIIREILPMVFVIIIVLFLITYIPDLVLFIPKLSGYK"
     gene            12120..13046
                     /locus_tag="PLQ30_05205"
     CDS             12120..13046
                     /locus_tag="PLQ30_05205"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPW01876.1"
                     /translation="MKQTIPDGVYPTLITPYKEDGRIDWDALGALIEWHIARGVAGFF
                     AVCQSSEMFQLSLHERLALARMTIRFVQGRVPVLVSGHVAETLDDQIEEAKMMADTGA
                     DALILVTNRLARRNENDEVFKKNLETFLGRFDRDILLGFYECPAPYKRLLNVDLMRFI
                     VEIGRFGFLKDTSCRIGDISIKIAAAKGSGFKLFNANAATLLASLQAGAAGYSGIMTH
                     FHSDLYVWMCNHWSDQQERARALQDFLGLASVIEYQLYPINAMYALQREGLPIQLISR
                     RADAQFFTESMRLEVDQLMGLSHRFSKAYREK"
BASE COUNT         3802 a         3124 c         3202 g         3573 t
ORIGIN      
        1 gtcatgatga aaatacgaca cccctttcga tcgagggcgt caaagtccac ttcttcctta
       61 gttatgccga cacaggcaac caattcgtgt acagctgttg tcttaccatg aggaatggca
      121 ataccatgtt tcatgccagt gctcatacga tattcgcgtt ccaggacact ttgaatcgtt
      181 tcttctttat ccagtacctt tccactgttt gatgcaagat ctacaagttc tctgatgatc
      241 tcttctttgt tcctgccttt gagatccaaa gaaatgagtt caggcttcaa tatttcctta
      301 aggtccatta ttctatccta atttgtgctg ttgatgccgt caagtatacc ccgcaagaca
      361 agtctgtcgg aggtcaaatg aaattgcgtg ctttcttgac gctgcgctga ccattcagtt
      421 cagcaataat cgataaggag aacatcaacc aagcagtatc aagaattgtc gcacagattg
      481 agagcggaag agcgatcggt accaaaagac tagctccatt ttgaaaaccc tgtccaaaaa
      541 gcacacaaac aagagagagc attacgataa tgtcgctacg tagagcagaa gctcccactg
      601 taaccgagag aggcagaagc gcgagcaata ggattatctg gccagcagtg ggattattcc
      661 tggaataaga caaaaacatt gctataaacg agcatgcaac aataattaca ctgcctaccc
      721 gaccaccgac aagaacgaga gggaatacaa ccgcattagt atcgcgcttt attcctaagc
      781 tttctgatac atgacgcaga gccgtagccg aagagataaa gatactcccc gaggctgcgg
      841 ctgtgagaag cggccccgac atcgcataga gaagtatata agggttggat tttcctccaa
      901 gaattcgata gacaagcggg aaaagcacga aaatcaagaa aaccgtcaga atcagcacaa
      961 ataaaagaac acttctatac tcagacggaa tccctgtatt tctaagattt agaaagagat
     1021 acaaagatat gggaatgagt agaattccca atatttccga tataaagaaa tttataaggt
     1081 aagatgctct agatatcaca tctagcaccg gcaatattgg cctcgacatc acaggatcat
     1141 gagagagggc taaaccaaag aatagagcaa atagcccaat tggtaggaac cattttgcag
     1201 aaaccgaaaa gacgtcgaat atactcgtag ggaatatggc tagaataagt tccaatggat
     1261 cgagtgtctg tacggttcca ctttgggaca ctaatggcaa tggcacaggc ttgaaaacca
     1321 agactacggc tatgccgata ccagccgcga ctaccaaact gacaaccgaa aggagcagag
     1381 attgagaaac gatctttgtg aagcgatgag cttccgacaa cctgaaaaca gaaataggaa
     1441 tacccactgt gagagtcaga atgagcgcat agcctccgat cttcaaagcc agctctgaaa
     1501 gaaaagaaat cataggcgtg ccatggatca tacttggagg caggaccaga tagagaatcg
     1561 ctcccatggc gataccaagg gcgtatttat gccatagctt catcttggga agcataactc
     1621 tattggattg acaggtcaat attgattaga tttctggaat tctctaatgc cgactgcgct
     1681 tagctttgca ttgacagatc ctattatttt caagtaatgt tcttttcaat gtgcgaccca
     1741 gtagctcagc aggatagagc gactgcctcc taagcagtag gtcggccgtt cgaatcggcc
     1801 ctgggccata aggaattaca acatatcatt gcttgtgatt tggcattgtt acttgagttt
     1861 tgttaccata taaaggatgg taatcaatta tgggtcacaa gcagatgccc atcgactatc
     1921 gagtttttgc aaggacaatc tcaggacaca agcgctttta tattcgattt ctttataatg
     1981 gaaagctatt tttagctatc aagcggaaat gcacagactc gaacgaggaa atcaggaact
     2041 tgaggaagat gttgaactct taaaaattgc tggtcttctt gcagcaaaaa aagtacggag
     2101 gttcaggtat ctggtgcaac aaaagtatcg ataccgaatc aagaaggcct gtaagacatt
     2161 gggaatctca cacagcggat tctatgctta tcgtgcacgc aaaccatccg accgacagct
     2221 cgaaaatgaa ttcttgtctc acaagataca cgaggtattc gaacagcatt ctggacgata
     2281 cggtgcaaag cgaattacct acgctcttaa ggatgaaggg atcaaggtca atagaaaacg
     2341 aatagtgagg ttcatgcttg cgatgggtct gtatacgaaa ggtgccaaaa gagcctacaa
     2401 gcactataat aagcaccacc gaacagtagc aaaggcaatc cctacgacaa tgcgctcatg
     2461 gaatcgttct ataagacgct gaaacgagag cttgttaaca atgctggatt caaaacaaag
     2521 gatcagacgc gtcaggcact gcttagtttt agagaattgt actacaacag cataagaaag
     2581 cattctgcgc tagggtaaat gactcctcag gaatatgagg acataaattt atagatttct
     2641 cttatctttg tgtccaaaat agttggaccc ttccatattg gagggagtgc tcatgaaaaa
     2701 gctagtacac aaacctacta atcaattaaa tgcatatttt atgttactgc tcacctctca
     2761 ttttatataa aacttcccca gcacaaataa ataccctttt tccttataga aatccaacct
     2821 acccagatca tcaaagatag tggctggagt atcctcgcgc accacataac ccagcccaaa
     2881 tccaaaagag tctgaaagca gaagatccaa tccaaccctc atggtaagcc ctataccgat
     2941 gcgtgagtta agcccggagc tcccatagta gctcttgaga taatcggtga aagctgcatc
     3001 ctgattcgtg gaagcaaagt gaaaataaaa tgcagggcca agtcccagat aagggcgcac
     3061 agatctcaat ggtgcaatga gattgatcga aaggggagca tagataaaag actcgtagta
     3121 cttccaatca gccttggatc cagtcttact cgacaggctc gtccatgaaa gaggtccttc
     3181 agtaaaggcc ccaccgcctg gaccgtagta gagatcgcca agatacatga catcggtgct
     3241 cactccgagc catggacgaa ccacatagct caccgtcaca ccatagagca tatcctgcac
     3301 attgaaagac gaaagagtat cagtaagcgg aggactaatc gccataaaac ctatacccgc
     3361 ctcccctccc aaattttgtg caaaagcaga aggaacaata cagataaaga gtatcacggt
     3421 tagaatcagc aaaaatttcc caaatttttt cataaaagcc ccctattcat tgcccataca
     3481 attattttaa atcttctttt ctcataaagc aaataaaatc aaaacaaggc aaaattgcta
     3541 tgaagtatct ttcgcccctg tgactctttc gatatgctga cgccatgaaa accacatttt
     3601 cagaaaaggc tgcttttatg tggcaggatg aattcggcgc tgccatcacc gtaatcgacc
     3661 gcgattatcg gattttatac atgaacgaca aatctataga agcgaacgcc acgcggggcg
     3721 gcaaagccct cctcgggcat gatgtgcgag cctgccacca ggagcgctcg gtgcgcatca
     3781 tcgaacatat tctggaaact ggcgaaccga acgtctatac catcgaaaaa caaggtgtgc
     3841 acaagcttat ctaccaagcg ccatggcgcc ataatggtgt tatcgcaggc gtggtcgagc
     3901 tttccatcga gattcccgca acgatgccgc actatgtgag agatgtacaa tagtgttgta
     3961 caacaatcat ccttgagacg gcaaaagcgc tttttgcgct cgaagggatg aatgtttcca
     4021 tgaacgatat cgcacgcaaa ataggcattc ccgtagggag tatctatacc tattttcctt
     4081 ctaaacaggc gctcatcgag acaattatcg aagaaggttg gaatcagttt cgcacgtggc
     4141 ttgaacaaca gctcgcgatg atcgaagata atctcgatga ttgcgcaatt ggtagcgctt
     4201 gaaacgctgc gcatttcagc tcagacagat ctcaatgtca gcgctgaaga tatcaaggtt
     4261 tttctaaaac agatcgttca agacgcgcta ggccagcctt tgcccgatac ggactaagcg
     4321 aacgcagcca cctctttgca aacggcgctc cgaccttcgc aaatggtgcg ataacacgct
     4381 cgcgccacgc gacctctgaa tagtaatctt cgagcgtctt actgcgaaaa ttgagaagca
     4441 gctctgcctc atcagaatcc gtaagccagc cacaatgaaa attgcgagta gcaaagagat
     4501 catcggataa aaacgatgag tctcccaagc caaaggcgcg catcattcga tcgagatagg
     4561 cgcgataggt cgtgcggcag aggataccgc caccaatatt gagtattcgg ccttctgtag
     4621 cgagggtaga ccatgcagca gcaaatgctc tgcctgcatc ttcggtatga actacctcaa
     4681 agtgtgttgc taacggaact tcgaacagca gcggatccaa gggtagccag tcgcttgcca
     4741 ccacataaga aagccgaaga atagtccaag gaagatcgcc tttttggagc atggcttcgc
     4801 actgctgttt ggtgagggcg tacgcgtcgt taggacagag tggatcctgc gcgctgatcc
     4861 acgggttggc aaggcggtcg ccatagagcg caatacttga ggcaaacaca atgcgcggag
     4921 gttttggaag gtgtttgcaa agcgcgatga cattctgcgt gccctcaaca ttgatgcgct
     4981 gcgcaagttc tggtgcgcgc gtggcaagtg gcgggataat ccctgcaaga tgcagcaccg
     5041 cccgcacatc cttcgattgc aaaaagtccc ctaaggcgga cattgttgcc gcatcgctga
     5101 catctccata gaaaattgta agcttccctt tcgcactgcc caaaagtgcg cggctttgac
     5161 gcgttctttt ctgacgggct gaaggatgct caaagataac gacttcatag cccatggcga
     5221 gcaacgcgcg gaccgcacga gatcccacat tccccaatcc cccagtgacg agtacgcgca
     5281 tagcgcctcc tttggtgtac tatttcattt tgcgattatt ataatgaatg aatgttcatt
     5341 cagcaataat agtgcacaga agaagagcaa aggggtaatg aaattctagt gttacgaacc
     5401 tttgattttt taaatcttcg cttggagtga tttccttttt ggggaaagac ggtgtagact
     5461 aaggcagagg atttgaatgc ttttgcacta ttgatgtgcg catgattgat tttgagtagg
     5521 ggccaacgca agtctgaagg gaggcgattt tatgcacgga aaacaaaacc ttacgcgatc
     5581 gtttgttctt attatttgcg ctatgggcgt ttttgttctt atgccagctt ttggccagtc
     5641 ttcgccgaat cttcgcggta ttgcaggcct tcagtctgga tcggtgttat ttctatcgat
     5701 taagggcgat gtgagtggtg cttcatcgct ttggggaacg gacatctata ccctcgattc
     5761 caatgttgct gctgctgcgg tgcatgcagg agttttagca aacggccaaa cagggattgt
     5821 ggcagtcgtt atttgtgatg ggctttcttc gtattctggc tccttcagga atggtgtaca
     5881 gagcaactca tggggaagtt ttgacctatc cttcaggttc gttaggcttg ctcaagctcc
     5941 tggaagcctc gcacaagcga cgcccagtgc gccaccagca acacaagcac cgcccactca
     6001 gcccgcaaag cccccggtag cgagtcgcaa tctctacgtc tttgatgatc caaatgtact
     6061 caaaggaaat ggcaacttta aaccaggcac cgttatctat gtgcaagtaa ctggaagagc
     6121 cgcaggagcg aagctttggg gaaccgatat ctataccagc gattccgatc ttaccggtgc
     6181 ggttgttcat actggtattc tttcggacgg gcagactggc attgtcaaag tgactattct
     6241 ccctggccaa cagagctatg tcggtagtaa ccgccataat gtcgtatcgt tgagttttgg
     6301 aagctgggct atgagttatt cgctcgagcc tgtgacgcaa tcctacgacc gcgttgtgcg
     6361 gatgattcta gaccccggtt cagcagacca tattcccaat gtgaccacag gcaacagcta
     6421 tgttgtctgg gtcacgggca gtgcagacca gcgctcggtt tggggtactg acatctacac
     6481 ttcagattct ccgtttgcga ccgcagcagt gcacgcagga atccttaaag taggggaaag
     6541 cgggcctgtc attgtgcatg tccttgaagg acagtcgcac tatgaaggct caacacgaaa
     6601 cggcgtcacc acaaagtctt ttggttcata tgggcgttcc tactcgctcg agcctgcacg
     6661 atgagattga aaagcactga gaatgcaaag ctcttaggac tatctacaca aaagtcgtag
     6721 gctttccaaa aaatccaccc agagcagaat ggcatggagt atgtaacaaa gttgtacaaa
     6781 gatagtgcaa cagcacgcag caattgaagg atctttcatg gaatgaaaca aaatacagtt
     6841 cgaaagcgac gactgcctaa ctgccgttca agccgatcga aggcgtgcct gactgcacgt
     6901 gtggttctgc ttgttagttt gcgtatcact tctagacagg ccgttcgttc catcagcgtc
     6961 agcagacatt cccgtgatcc cttttgccca ctatacacgc attagaataa cagtcacgaa
     7021 tcagcatggc gatgcgctgt atcagtgcgt agtgcgtact cgacttggga agtggtcctc
     7081 tggaccgcat ttgttccgct gcgtccagct gtgcatgcta tgcattgtat tcctttggga
     7141 aaaaaggaat atttgaaaat atatgctcta gcacactgtg ttacttgttt tttggtaaaa
     7201 cccattgtag cggagttccg tggggctttc ttttttctcc ttgttggttt tttaaaatgg
     7261 acactttgcg ctgctgctca cccagatttc tctatgtaga aaatttttct tgacactata
     7321 cgattctcat gagataattc atatatagga aatttgttca tatatatgta cacttcttag
     7381 ggaatttagg atggcggaag tcaactcagt tcttaaggct tttgcaattc tcgatcttct
     7441 ctctgatgga aaaaagcgaa gtctctcgga tattgcacat gagctttcgc tcccaaaaag
     7501 cactgtgtct aatctactag aaacgcttac ttctgaaagg gtattggagc gcgattatgg
     7561 atcaggacta ttttcacttg gtatcaggtt aattgaactg ggctattgta cgcagacagg
     7621 gaatgatctt gtgcgcatcg cagctccttt tctgcgcgga ctcaatcagc gattcgatga
     7681 gacagttcat ctcgttttgc tcgatcgcga tgaggtactc tatattgatt gtattgaatc
     7741 gaagcgccgc ctacgaacct attcagtcat aggtattcat gctcctcttc attgcacatc
     7801 ggtaggcaaa gctattctcg cttttttgcc taatgctgag atacagcgta tcctcaaaaa
     7861 taaaggtttg ccatccttca ctcctcatac aatcaccact gaagatcgtc tctggagaga
     7921 aattgcagtt attcgcgaac gtggctatgc gatcgatgac atggagcatg aggatcatct
     7981 gcgctgcgta ggggctccga tcttcaattc gaaaggggag gtctttgcat cggtgagcat
     8041 atcaggccct gcagagagaa atacgctcaa gcgcctcgaa ttgatgactc cagcgctctt
     8101 ggaggcaact gccgaaatat cacatcggct cggatatcga aacaaggatc atagtcgtgc
     8161 aagcagctaa aaagtgcaat ccactttaag gaggtcttta tggcaaaatt tgtaaaagag
     8221 tactatggca agatactcat acctatgctg acacctttcc gagaagatgg gagtgttgat
     8281 tatgaaaaag cagcagcact tgctgaattc atcataaatc aaaagaaagg cgacagcctt
     8341 gtccttgcag gctcaacagg cgaattctat gccatgagat tcgacgagag ggtaagatta
     8401 ttcgaaacta ttaaagaacg cgtcggcaat cggatcccca taattccagg tgtcggagca
     8461 gcatccacca tcgaaacaat acaacttgca aaaaaggctg aagaaatagg ctttgagaca
     8521 gtgatgattg ttgctccata ttatacaaag ccaaaccagg aagaactcta tcgccatttc
     8581 atgcaggtgg cgacatcagt gcctgtcaac atactgctgt acaatattcc aatttatacc
     8641 ggcgttaata tagaacccgc cactctatgc cgccttgcga agcagcctaa tatagtagga
     8701 attaaagagg aggcagaatt gaatcctaaa caaatcaccg cttttctgaa cgctacacct
     8761 gaagaatttg ttgtctacaa tggcgatgat accatgatcc tcgaggccta tgctcagggt
     8821 ggcgaaaagc gcatcggggg agttgtgtca ggatcatctc acattatcgg gcatcttatc
     8881 agatcgatga ttgaagattt tctcgcagga aaagtccaag cagcagcaga gcagcaacgg
     8941 aagatttatc catttttgaa atgccttagc caaaataata gaacaaatgg agcaccactc
     9001 ttgaaagaag cgcttcggct ttatggattc gatgcaggat atcctcgcgc gccactcatt
     9061 caaggtagtc cagaagaaat tgagaatatt cgcatagcca tggtggcggt tggtgtcttg
     9121 aaagcaggat aatttagcca ttcaaatggc tagaaattga ggattaatct aaggaggttc
     9181 attatgaaga agtgtattgt tgcagtgctg attatgttgc ttgttgccag cacagcagtt
     9241 ctcgctcagc aaaagcctgt caagcttgtg ttcacatcag tcagcgtacc aaccgatgcg
     9301 catacacagg caatgtacgt tttcaaggac gaggtggaaa aactctcagg tgggcaaatt
     9361 caggtcgacg tctatgattc tggaaagcta tttactcagc aggccgagca ggatgccatt
     9421 cgtaagggaa cagtcgatat ggtctatagt tctgcatcgt ggcttgcaga atttgtccct
     9481 tacctttcga tgtttggcgc agcctataca ttccaaagct atgatcagat gacgaagact
     9541 tttaatggcc ccatcggcaa gaagatattc gaagatgtgg ccaagaaaac aggtgtccgg
     9601 ccgctctgcg cattctatct tggtactcgt cagctcaacc tcatagcaaa agttggccca
     9661 gtcacaaagc ccgagcagat gaaaggtgtg aagttgcgtg ttccgcagag tcctacctgg
     9721 atcgctatgg gcaaagctct tggagcaaac ccaaccccaa tggcctttaa cgaattgtat
     9781 atgggtctca aaaccggcgc tgttgatggc caggataatc ctctcccaac agataagaat
     9841 gccaaatttt atgaagtcac caagtacata gttctcacta atcatctggt tgactcagta
     9901 tggccttcta tcaatgagaa aaaatggcaa agcctgacaa agcaacagca agactggatt
     9961 atgcaggcac tggagaaagc acgccaattc tgcgataaaa caaatctcga taacgagaag
    10021 gaaattctgg atttcttccg tcagcaaggc cttattgtta tagaaaatcc tgatcgagat
    10081 gcatttgctg catatgcaaa gaatttctat cttacagaag gcaaagatat ctcaaaagac
    10141 tgggatatgg cactctatga tgaaatccaa aaattgaaat agtgcaggat gattgcccgg
    10201 agcaaaatat gcgccgggca attgctctgc tttgtgaccg tcgctttgtg gttgtcgatt
    10261 gaacctatcg atgtctttgc gccaatgcct ttacagaggg cagattaaga ggaggcagta
    10321 tgccagactg gctaaaaaag acaggcaaat tccttgtcga ctgcattgaa ctctatgtac
    10381 ctatactgac atttagtacg ctttttgtgt cctttgtgat ccaaatattt gcgcgtaaat
    10441 ttttcaagcc gctgatatgg ccagaagaat tgagcctcat ctgttttgtg tggactgcac
    10501 tgcttggagg cctttactcc aaacggatgg gcacacaggt tgcatttacc atgctctacg
    10561 atgcagcaaa accagaaacc aaacgcatta tgcgcattgt gggaaatgcg ctgcttctca
    10621 ttgcttttgc gattattctt gttccttcat ggaattatgt acagttcatg gcctataaaa
    10681 aatcggatgc actgcggatt cccatgaata tcgcatattt cccttttgtt gtgtatctgg
    10741 tggatatgat tgttaggctc gctcttgaga ttgtaaaaga atttactact ccagaagccc
    10801 caggaggcgc agaatgaaca ttccgctcat tgtattcgtg gtttcattcg ttgcggtatt
    10861 cttgcttggc attccccttg cgccgggtat gatgctttcc agcgccttct attttctgct
    10921 ttccgataac cctgctgcgt cactcgacat ggtcgcgatt cagttcctaa ccaatttgaa
    10981 tgcgagcttt gtgctcattg cggtgccgct ctttgttttc atggctgaga tcatgaattc
    11041 tggtaaggtc actgaaatga tcttttcatt ctcgagcgca ctcgtcggca agcgaaaagg
    11101 cgcccttgca catgtcaata ttatcaattc tatcatcttc tctggaatga ccggctcagc
    11161 gcttgcagat gcctcagggc ctggcaatat ggaattaaaa gcgatgcagc agcagggcta
    11221 tgatgatgcc tacagttgcg ccatttcagc agcgagcgcg attatcggac caattattcc
    11281 gccaagcatt cctatggtta tgtacgccat gctttcgagt acgtcgatag gctatctgtt
    11341 tcttggggga atcattccag gcttgctcat tggaatagcg cttttgatat ataatgcaat
    11401 tatttctaat aaaagaaatt atccagaagg gaagagcttc cctcctcgag aattttggaa
    11461 atttacttta caagcattgc ctgcgctttt tactgtcgtg attttattgg gaggaattta
    11521 tactggtgtc gttacaccaa cggaagcagg agcgctcgca gcattctatg gaatcctcat
    11581 ttcagttttt gtctatcgag cgcttggctg gaaagaactc aagcagtgcc ttatcaacgc
    11641 agtgcgcacc accgggacgc tttcgcttct tgtaggaagc tcatttgcat tttcctatat
    11701 tgtgagcatt gagcaaattc ccaatgtgat tgccacattc ttgctcggcc tcactcagaa
    11761 caaatatgta atgctcttca ttctcaatat cgccttgctg attcttggat gtttcctcga
    11821 taccacgcca atccagctca tatttacccc aattatcatt cctattttga gccagcttgg
    11881 cattgacctt gtgcatttcg gcgtggtgat ggtgctcaat ctcatgattg ggcttgcaac
    11941 acccccctat ggtatgcttc tctttactgt ttcagggctt tctggcgcac ctctgaagga
    12001 tataattaga gaaattttgc caatggtatt cgtcattatc attgtcctat tcttgataac
    12061 ctatattccc gatcttgtac tttttattcc taaactatca gggtataaat gaggtcatca
    12121 tgaaacagac aattccggat ggtgtgtacc ctacccttat tacaccttac aaggaagatg
    12181 gcaggatcga ttgggatgcg ctgggcgcgc ttatagagtg gcatattgcg cgtggtgttg
    12241 ctggattttt cgctgtttgt caatcgagcg agatgttcca gctcagcctg cacgaacggc
    12301 ttgcacttgc tcgcatgact attcgatttg tgcaaggccg cgtacctgtg cttgtgtctg
    12361 gccacgtggc cgagacgctc gatgatcaga tagaagaagc aaagatgatg gccgatactg
    12421 gcgccgatgc cctgatcctc gtgaccaacc gcctcgcacg ccgaaacgaa aacgatgaag
    12481 tgttcaaaaa gaatctcgag acgtttcttg gccgattcga tcgcgatatt cttcttggtt
    12541 tttatgagtg ccctgcaccc tataaacggc ttctcaacgt tgaccttatg cgattcatcg
    12601 tggaaatcgg tagatttggc tttctaaagg acacaagctg ccgcattgga gatattagca
    12661 tcaaaattgc ggcggcaaaa ggcagtggct ttaagctctt caatgccaac gccgcaacgc
    12721 tgctcgcctc gctccaggct ggcgctgcgg gctattctgg cattatgaca cattttcact
    12781 cggatctcta cgtgtggatg tgcaatcact ggagcgacca acaagaacgg gcgcgcgcac
    12841 tccaggattt cttgggttta gcctcggtga tcgagtatca gctctacccc atcaacgcaa
    12901 tgtatgcgtt gcaacgagag ggcttgccca tacagcttat ctcgcgccgc gcagatgcgc
    12961 agttctttac tgaatcgatg cgcctcgagg ttgaccagct gatgggactg tcgcaccgat
    13021 tttctaaagc ttatagggaa aagtagagaa tgggatacac agccatgagc attgccacgc
    13081 tttatgaatg gaggggtgat aaactggaca tgaaatgaag agaaagagga agggttcatg
    13141 tccagaagga agttcaacaa ggagtttaag gtagcagctg ccaagattgt gctcgatgat
    13201 gagtagctgg tatctcaagt agcttaacaa ctttcagtgc atccgaatag tctctaccgt
    13261 tgggcgagcg agcatgagaa gtacggcgag agtgtcaagg tgtggctggg agacattacc
    13321 tatgtttcca cgcacgaaag ttatctgtat cttgcggcat tcttggacct gtttacacgc
    13381 aagattgtcg gtaggtcgat atcgtccacg atgactgagc cagattcaag acaaaggatc
    13441 aggcgcgaaa ggcacgggtc agctccatag aattatatta caacagcgta agaaagcatt
    13501 ctgcgcgagg gtatatggct cctcgggaat atgaggagct gaattcatag atttctctta
    13561 tctttgagtc caaaatatct ggacccttcc agttattcat tttctgcacg ggcctgttca
    13621 gcgggctcag catgcctggt atttgcgccg agtctggcaa ggtaagtggc gagcagcgat
    13681 ttgaacattt tgccgtgatt t
//
LOCUS       DAOMZL010000047        13689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_4114_length_13689_cov_14.446897, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000047 DAOMZL010000000
VERSION     DAOMZL010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 13689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13689
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4114_length_13689_cov_14.446897"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /gene="secG"
                     /locus_tag="PLQ30_05210"
     CDS             <1..332
                     /gene="secG"
                     /locus_tag="PLQ30_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPW01877.1"
                     /translation="VIIQDEESDSIGGIFASGAQSAFGSRTSNIVVRITYALGALFFV
                     IAFSLAILNKSSTGNIQKVVEQESAQTATSEWWNSQSEEDQSVQGQLAPQDQTVQPEG
                     TNTPATK"
     gene            477..875
                     /locus_tag="PLQ30_05215"
     CDS             477..875
                     /locus_tag="PLQ30_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01878.1"
                     /translation="MRIGINAIGSRKLSSVSNAMLHSLKQKGHDSVLTSDYSYSLGSF
                     DFLIFVTEPKGMFGGLLSSVPQDLAKSGDLVGKRCLAMVQKRGFRSGHTLKKLMEALE
                     HEGLVIVQGEVFSNTDTAIHIIAEVPLKRG"
     gene            879..1733
                     /locus_tag="PLQ30_05220"
     CDS             879..1733
                     /locus_tag="PLQ30_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPW01879.1"
                     /translation="MKNYYEILGVEPDSSPKDIKSAFRRQAKRLHPDMFYSKEKARSE
                     ESTARLRESAMRLILEAYKILSDAEKRRSYDRELRRQEKENKGFDYREFLKMRADDPQ
                     SQARLIVFDLLHGFEEEALWIYERSKGFQDFRLERWLERGEAMDCEYCIAEEYEKRGK
                     YIKAYQIYKKLIQMELEKPWFRYYFDVVALQFRLLVLQKLPGKIDDDDYLDRLEEAIE
                     LGISPRETAQYLRKKVEILIHRGEADRAAEALLQISQIYPKLAGFDSLRVKVERALGQ
                     QVVQDRVY"
     gene            1786..2817
                     /locus_tag="PLQ30_05225"
     CDS             1786..2817
                     /locus_tag="PLQ30_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPW01880.1"
                     /translation="MKRIATWFCIFLFSAISVFAQTSIDKPAATIKLIKQEVISVRQL
                     RTDVEKLENSVGVKLTLDQRKDVLDARINSMLFLQFCEREKISISEAQVNAALAQLRS
                     QLGTNATDADLEKSLRASGVFVDPATFVRQRLLFETYIQTKRQDELKIALKAPTADEI
                     LKAYDLTKASLIRPDTIRISVIYVDTKGKSNGEIAKAKELMNSISASLKSNPSKFDEY
                     VLRASDQAGYKAIPSMYLEKTQQSKTIFGDEFFNVAFQANVGEITPVIETPVGYRIIR
                     VNEFLAQKQLTLSDPVPGNQNLTVQEFLAIQLASEKQQAFLNKAEADLIEVLRKEATI
                     KIYSENLNW"
     gene            2830..3267
                     /gene="nusB"
                     /locus_tag="PLQ30_05230"
     CDS             2830..3267
                     /gene="nusB"
                     /locus_tag="PLQ30_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPW01881.1"
                     /translation="MASRRKARILAVQAIYAWDMSHQELEDILSFEWLDEDKKARYEI
                     EILDFAKLLIIGTIEQIDSIDDLIKNHLQHWAFERLRKVDLAILRVGTYSLIYQQDVP
                     AQISIDEAIEIAKEYGSDDSYRFINGVLDGIRKDFFGEFHDSN"
     gene            3254..4000
                     /gene="map"
                     /locus_tag="PLQ30_05235"
     CDS             3254..4000
                     /gene="map"
                     /locus_tag="PLQ30_05235"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPW01882.1"
                     /translation="MIRIKNERQIEGIRSSCDLLSLLYERLKPQVQAGNTPKDLDRFA
                     YDFIIKHGGKPAFLGYEGYPATLCISVNEVVIHGIPNDKPFQEGDIVGIDSGINLNGF
                     FSDAAITLPVGSIAQEAAKLLKVTRESLELAIERLKPHVRISEISRAVFSHATKNGFN
                     VVRQYCGHGVGLEIHEDPQIPNYVSPGPNPRLMSGMVLAIEPMINIGTSDVRVLDDDW
                     TVVTMDGSLSAHFEHTVLITDSGHEVLTRW"
     gene            4118..5629
                     /locus_tag="PLQ30_05240"
     CDS             4118..5629
                     /locus_tag="PLQ30_05240"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HPW01883.1"
                     /translation="MSLRKRLYSRNFFVANLVILGIVVGFGLAFLFRAPPSGSSSLPV
                     VKAETPSMVTNPDTEASIETAELVQNALHNIAKTVLPAVVELDVVEGGQQTQQQAPSF
                     PFYFFFGPDSPFSDQFQPQEGLGSGVIVRRTGKTVYILTNHHVAGNAAKITVKLSDGR
                     EYEGKLVGSDERKDIALVKFETDDSDISIAKLGDSSKLQVGDLAIAVGSPFGYIASVT
                     AGIISALGRTGGPDENINDFIQTDAAINKGNSGGALVNIKGEVIGINTWIASPSGGSV
                     GLGFSIPINNVKDAIDDLITNGTVKYGWLGVSLSNISQDKASAKELGLEGKKGAFISH
                     IFLNGPADKAGILPGDFITAVNGKAIQSTDELVRVVGDIKPGSTAKLEFIRSGVVMTA
                     STKIELRDNAIASNNANIFPGVTVISLKSDSIDQKKIPSGVRGALVIDVLAKSPAASM
                     GLQSGDIITKLNGKNIDNLRDFYELLAKESEKKLVFTVNRNGQTVETLAYVRK"
     gene            5714..6346
                     /locus_tag="PLQ30_05245"
     CDS             5714..6346
                     /locus_tag="PLQ30_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HPW01884.1"
                     /translation="MHKEFVTYDTVRNNAIKLAYRIYRDGFIPDIIYVSLRGGAYMGN
                     VISEYFKFVKDNEHPVFYAAVVARSYTGFDQQESIKIDGWTYNPDFLRHGDKVLLVDD
                     VFDSGRTINYLARVIMERGLPRSDVKIAVHDYKVREYVPQHLPILPDYWCRKIEVKSR
                     EDELWIHYMSHELEGLTQQEIAEHYLKDDPSLAEPMSFVAKESTSSTSDK"
     gene            complement(6358..7632)
                     /locus_tag="PLQ30_05250"
     CDS             complement(6358..7632)
                     /locus_tag="PLQ30_05250"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HPW01885.1"
                     /translation="MAEILSIVLGGGKGTRLFPLTKERAKPAVPFGGKYRIVDIPLSN
                     CINSDFRKIYILTQFNSASLHLHIYRSYNFDRFSKGFVEILAAEQTHLHSGWYEGTAD
                     AVRKNLIHFRPHQPTHYLIVSGDQLYRMDLGEMFKQHVESGSRLTVAGTLVDRDTATS
                     LGIIKIKENRSIDSFLEKPGPTKDIHEYEVPKSLRPEGASPNKPYLASMGIYIFDAPL
                     IEETMQLDANDFGKEIIPFVVSKEKVNTYVFDGYWEDIGTVRSFYEANIGLTDINPKF
                     NFYDEQSPIYTHARNLPASKLNYCTLNQALASDGCIITNASITRSIVGIRTVIESGAS
                     LDGVVCMGSDYYENAEEKEENRRKNIPNIGIGGGSIIRHAIIDKNARIGSNCRIGIDP
                     LARQEGEYRTYYIVDGIIVIPKNQIVPDGTVI"
     gene            7696..10011
                     /locus_tag="PLQ30_05255"
     CDS             7696..10011
                     /locus_tag="PLQ30_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW01886.1"
                     /translation="MNSESANAIYSEFLSWLAERNEIAWSSILPPKSADCMPFPEDLS
                     PILSEALHRRGIDSLYTHQRKSYDLVRSGHDVVIVTPTASGKTLCYNLPILQTLLEMP
                     KARALYLFPTKALAQDQQSGLNEVMLAGSIGFPVNTYDGDTPSDIRSRARTDGRIIIS
                     NPDMLHAGILPNHTKWVKFFSNLKFIVMDELHAYRGVFGSHVASVIRRLLRIAKFYGS
                     SPTFIFSSATIGNPQELAEHYLERKVEMIEDNGAGSGEKILVCINPPIVDEVQGLRRS
                     NALEAESAMLWLLQKGVRTILFSRSRLQVELLAHYLNQKLENPFNRNYGIVVKPYRSG
                     LLPSERREIERGLRNGTVHGVVSTNALELGIDIGGLDAAVITGYPGSIASFWQQAGRA
                     GRTSGISLAIYVASSSPLDQYFVAHPEYFLSRGAEQAHIDTYNPYIFTDHLKCAAFEL
                     PFSEYEAFSPDNSNRRAAELTIEALECLEEEGIVRHTEGRFFWSAEGYPGEKISLRSA
                     TTENVVIVDISNGHHDVIGEMDRPSAKELLFEKAIYMHLGSQYQVKKLDFEKRICLVE
                     ASDADYWTDSIVKRDIALLTEDSVESYSQFRLILGDILVRSQVEKYKKLRFNTHENIG
                     YGEIWQEPEEMQTRSLMMVIYPQSQSAQFLSQFGQESANSLLYGVANLMRQLAPAKIL
                     CDVRDIGVASRARDPFYESPVLYLYDMYPGGTGIAEALCSNMSGIIGSTIERIASCTC
                     LVGCPSCIGVENANPDNKAASLRLLELLSCS"
     gene            10086..11375
                     /locus_tag="PLQ30_05260"
     CDS             10086..11375
                     /locus_tag="PLQ30_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HPW01887.1"
                     /translation="MNLKERLALIKSNEKNNKGNSAPRPRKMPDNWRNILPYVWIREI
                     QGPIFIVPPFFYSGIIRSARPSERFSEQTSETEGDIDSQGSAFRIPADRVIFFDLETT
                     GLSGGAGTYAFLSTTARFEGRNLVLNQVFLEDYPGERDFLSMVISQLSAGDWIASYNG
                     ATFDIPLLRSRCILNGIVMPIRMHVDVLHDCRRFWGKILDSCSLASMEKFVLKSTREF
                     DIPGSEIPRVWLDYVKSDFLSENQKALMELVWQHNIMDVVSLARLFLHIESLYRDPYR
                     AVIEDSVDPLSLANRICKLGRLEEAKSLLLMIYRNNKEHDLSREIIREVQRYLAKLAR
                     KDKDLDLFSELVLSMDSEFLYGCVAKAKLFEHTFKDEKTALVWAQKAHDLACNSVNSG
                     TIKRKDKEMAAQLSVIASLDHRIARLERKIANRKSIP"
     gene            complement(11301..12401)
                     /gene="rlmN"
                     /locus_tag="PLQ30_05265"
     CDS             complement(11301..12401)
                     /gene="rlmN"
                     /locus_tag="PLQ30_05265"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPW01888.1"
                     /translation="MNYDSADRLSAVYPSGLSQEELAAILSEEPLYRAKQAFAWFSRG
                     VPSFEKMTNLPVTLRERLAQNFGGSVRSSVVQNEFSDKDGSLKLQIRLRDGASVECVL
                     LEDIEGRKTACLSSQVGCAMGCAFCKTGTLGFLRNLGPDEIVEQFHHLRERRGFISNV
                     VFMGMGEPLLNLENVRKSISILSHPAGIGLSMRKITISTSGIVPAILDLAKNGPNVRL
                     AVSLTSANPDLRTELMPVNRKWPLDDLKSALLEYQQDTNARITFEVVLLGGLNVGEKD
                     AIELVNWITPIKSQVNLIAWNPIDGMPFKTPSVRQIEEFSRILSDNGIVTVRRMSRGR
                     GVMAACGQLGDTLSICNLALEPSNTMIKRGND"
     gene            complement(12398..13534)
                     /locus_tag="PLQ30_05270"
     CDS             complement(12398..13534)
                     /locus_tag="PLQ30_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW01889.1"
                     /translation="MPSLPYNDDFFEIPNEIQLENTEVAEISKKGYQLLKENRFDEAV
                     ECFVQILEKDKENNYALVGLGDAARKQERFKEAADYYRRCLVFHPGNSYALFGLADCY
                     KALNQFQKAIEIWEQYLLHDNTNITVLTRVADAYRKVRDFRKSKSIYHRVLELNPDNH
                     YALIGLGHLHYDFKEYKDALSYWQRMVELEGDNVDIRVLTAIGNCYRKLKHFDKGIPI
                     FEKALQKEPDNFYALFGIADCYRGVGKQNMSLLYWNKILERDPKNKVILTRAGDAYRN
                     MGDFEKATEYYQNALNIEFDIYAVLGLAVIARQQGKYDEAIASLRSLLQNDPKNYRIY
                     VELAQTYLLNNQRQMAIEILTEFQQMGIKNPVIQDALFKLTAKT"
BASE COUNT         3883 a         2815 c         3156 g         3835 t
ORIGIN      
        1 ttgtgattat ccaagatgaa gaatctgatt cgataggggg gatttttgca agcggggctc
       61 agagtgcctt cggttctagg acatcgaata tcgtggtccg gataacctat gcgcttggcg
      121 cgctattctt cgtaattgca ttttcccttg cgatattgaa taaatcttcg acaggtaata
      181 tccaaaaggt agtggagcaa gagtctgccc agacagcaac cagcgaatgg tggaattccc
      241 agagcgagga agatcagtcg gtccagggtc agctcgcgcc gcaggaccag acggtccagc
      301 ctgaggggac taatacacct gccactaaat agctctattc agctcaattt gaaaggcagg
      361 aagctaaagg agggcacgga agaatgtaat tccgtgcttt attctatgaa taattagcat
      421 gaaatagcgt attctatgcg taacttgcta attcatatag atgtttttta attattatgc
      481 gaattggaat aaatgctatt ggatcaagaa aactctcttc agtttctaat gcaatgttgc
      541 attcattaaa acagaaaggt catgattcag tactgacatc agattattcc tatagcctcg
      601 gctcctttga tttccttatc tttgtaacag aaccaaaggg tatgtttggt ggtcttctgt
      661 cctcagttcc tcaggatttg gcaaaatcag gagatttagt gggaaaacgc tgccttgcta
      721 tggtgcaaaa gcgaggattc agatcaggcc atacccttaa aaaattaatg gaagcactgg
      781 agcatgaagg cttggtgatt gtacaaggcg aagtcttttc taatacagat actgcgattc
      841 atataatcgc ggaggttcct ctgaaacgcg gatgagccat gaaaaactat tatgagatac
      901 ttggtgtaga gccggattca agccccaagg atataaaaag cgcatttcga agacaagcaa
      961 agcgtctcca tccagatatg ttttattcca aagagaaagc cagatccgag gaatcaaccg
     1021 caaggctccg cgaatcggct atgaggctta ttcttgaggc atataagata ttatccgatg
     1081 cagaaaagag aagaagctac gatagagagc ttcgaaggca ggagaaggag aacaaaggat
     1141 ttgattatcg cgagttcctt aaaatgcgag cagacgatcc tcaaagccag gcaagactga
     1201 tagtctttga ccttctgcat ggtttcgagg aggaggcact atggatctat gaaaggagta
     1261 agggctttca agatttccga cttgagcgtt ggctcgagcg cggcgaagcc atggattgcg
     1321 agtactgcat tgcagaggaa tatgaaaaac gaggcaaata cataaaggct taccagatct
     1381 acaagaaact aattcagatg gagctggaaa agccatggtt tcggtattac tttgatgttg
     1441 tcgctcttca attcaggttg cttgtcttgc agaaattgcc tggtaaaata gatgatgatg
     1501 actatctgga caggctcgaa gaagctatcg aactagggat ttcgccaaga gagactgcgc
     1561 aatatcttag aaaaaaagtc gaaatcttaa ttcatagagg agaggcagac agagccgcag
     1621 aagctctgct gcaaatatcg cagatctatc ctaagcttgc tggctttgat tcattgagag
     1681 taaaagttga gcgtgctctt gggcagcagg ttgtacagga tagagtatac tgaacagcac
     1741 ggtatagtgc ttgtaaataa ataatattga gagcaggcta atgatatgaa gagaatagca
     1801 acttggtttt gtatctttct gttttctgcg atttcggttt ttgcgcaaac gagcatcgat
     1861 aaaccagcgg caacaataaa actcataaag caagaggtta tttcggttcg ccagttgcgc
     1921 accgatgttg aaaagctgga gaattccgta ggtgtgaagt tgacattaga tcaaagaaag
     1981 gatgttctcg atgcgagaat aaatagcatg ctgtttttgc agttctgcga gcgagaaaaa
     2041 atctcgatat ctgaggctca ggtaaatgct gcattagcac agctcagatc tcagttgggt
     2101 acaaacgcca cagatgctga ccttgagaaa tcacttcgcg cttccggcgt atttgtagat
     2161 cctgcaacct ttgttcgtca aagactgctt ttcgagactt atattcagac caagagacaa
     2221 gatgagctaa agattgcgct caaggcgcca acggcggatg aaatcttgaa agcctatgat
     2281 ctgacaaagg cttcgcttat aagaccggat acgattcgaa tcagcgtgat ctatgtagat
     2341 actaagggga aaagcaatgg tgagattgca aaagcaaaag agcttatgaa ctcaatctct
     2401 gcctctctca aatctaatcc ctcaaagttc gacgaatatg tgcttcgcgc aagcgatcag
     2461 gctggataca aggctattcc ctctatgtat cttgaaaaaa ctcagcagag caaaacgata
     2521 tttggcgatg aattcttcaa tgtggccttt caagcaaatg ttggtgaaat aactcccgtt
     2581 atcgaaacgc cggttgggta caggattatc cgtgtcaacg aattcttggc tcagaagcaa
     2641 cttacactct cagatcctgt tccgggaaat cagaatttga ctgtgcaaga gttcttagcg
     2701 attcaacttg cctccgagaa acagcaagct tttttgaaca aggctgaagc ggatctgatc
     2761 gaggtgctgc gcaaagaagc aactatcaaa atatattcgg aaaacctaaa ttggtaaggg
     2821 acatttctgg tggcttcgag aagaaaagca agaattcttg cagtacaggc tatatatgcc
     2881 tgggatatgt ctcaccaaga acttgaggat atattatctt ttgagtggct cgacgaggat
     2941 aaaaaagcac gctatgagat cgagattctt gattttgcta aattgttgat tattgggact
     3001 attgagcaaa tcgattctat cgatgattta ataaaaaacc atcttcaaca ttgggcattc
     3061 gagagacttc gaaaagttga tctggccatt ctaagggttg gaacttacag cctgatatat
     3121 caacaggatg ttccggcaca gatcagcatc gatgaagcga ttgaaatcgc taaggaatat
     3181 ggtagcgatg actcttatcg ttttatcaat ggtgtgctag atggcataag aaaggacttt
     3241 ttcggagaat tccatgattc gaattaaaaa tgaacggcag atcgagggaa ttcgatcgtc
     3301 atgtgacttg ctgtctttgc tctatgagag gctgaagcct caggtacagg ctggtaatac
     3361 gcccaaggat ttggacagat ttgcctatga ttttattata aaacatggag gtaagcctgc
     3421 ttttcttggt tatgagggct atcctgctac cctctgcatt tctgtcaatg aggtggtcat
     3481 tcatggcata cctaacgata agcctttcca agaaggcgat attgtcggca tagatagtgg
     3541 tataaatctg aatggttttt tctccgatgc tgctattaca ttgcctgttg gatccatagc
     3601 tcaggaagcg gctaaattgc taaaagtaac aagagaaagc cttgagctcg caatagaaag
     3661 actaaaacct catgtgcgca tttctgagat ctctagagcg gttttttccc atgcaacgaa
     3721 gaatgggttc aacgtggtac gacaatattg cggtcatggg gttggactgg aaatccatga
     3781 agatcctcag attccaaact atgtctcgcc tggccctaat ccaaggctaa tgtcgggaat
     3841 ggtgctggca atcgagccaa tgataaacat aggcacaagc gatgtacgtg tactagacga
     3901 cgattggacc gtcgtaacga tggatggttc gctatcagct cattttgaac atactgttct
     3961 cataactgat tcaggacacg aagttcttac aagatggtaa tattctttca tttctttttc
     4021 taatttttta tctcttgtac agttcatggg agagtgcagt tttattatat ttgatacaga
     4081 ttgcaaatac tgtgccaaat aacggaggat atcgaacatg tctttgagga aaagattata
     4141 ttcacggaat tttttcgtcg cgaatcttgt aatacttggg attgtggtag gatttggcct
     4201 tgcattttta tttagggcgc ccccctcagg aagctcttct cttccagtag tcaaagctga
     4261 gaccccatcc atggtgacca atcctgacac tgaagcttca attgaaactg cggaattagt
     4321 gcagaatgcg ctgcataaca tcgcaaagac agtacttcct gcggtagtag agcttgatgt
     4381 ggttgagggt ggtcagcaga cccagcagca ggcgccatca tttcctttct atttcttctt
     4441 tggacccgat agtcctttct ccgatcagtt tcagccacaa gaaggcctcg gctctggtgt
     4501 aatagtgcgt agaactggca agacagtcta tatcttgaca aatcatcatg tagcaggaaa
     4561 tgcagcaaag attacggtaa aattgagcga tggccgcgaa tacgaaggta agcttgttgg
     4621 atccgatgag cggaaggata tagccctcgt aaaattcgag acagatgatt ccgacatctc
     4681 gattgcaaag cttggcgatt cctcaaaact acaagttgga gacttggcaa tagcggtagg
     4741 tagccccttc ggttatatcg cttcagttac agccggcatt atcagcgcat tgggcaggac
     4801 cggaggtccg gatgagaata tcaatgattt tattcaaacc gatgccgcaa ttaacaaagg
     4861 taattccggt ggggctcttg taaacatcaa gggggaagta ataggcataa atacgtggat
     4921 tgcatctcca agcggaggat cggttggcct cggtttttct attcctatca ataacgtaaa
     4981 agatgcaatc gatgacctta tcacaaatgg cactgtcaaa tatggctggc tgggtgtaag
     5041 tctctccaat atctctcaag acaaggccag tgccaaagaa ctaggtcttg aaggcaaaaa
     5101 gggtgctttc ataagccaca tttttctcaa tggtccagca gataaggctg gaatcttgcc
     5161 tggagatttc attacggccg tcaatggcaa ggctattcag agcaccgatg agctggtaag
     5221 agtggtaggc gatatcaaac ctggttccac cgctaaattg gaattcatcc gaagcggggt
     5281 tgtaatgacg gcgagcacaa aaatcgagct tagggacaat gcaatcgcat cgaacaatgc
     5341 taatatattc ccaggtgtaa ctgttatctc actcaaatcg gactctatcg atcagaaaaa
     5401 aatacccagc ggagtacgag gggctctcgt tatcgatgtt cttgcaaaat ctccagcggc
     5461 atcgatgggt ctgcagagtg gggatattat aactaagctc aatggcaaga acattgacaa
     5521 cctcagggac ttctatgaac tgctagctaa agaatccgag aaaaagctag tcttcactgt
     5581 caatcggaat gggcagacag tcgagaccct tgcttatgta aggaagtaag tatatatttc
     5641 ttttctagag aaagtcattc gataagtgaa gcctccataa gggggcttca cttgtttttt
     5701 cggaagggat tctatgcata aagaattcgt aacttatgat acagttcgaa ataatgccat
     5761 caagctggct tataggatct atagagatgg cttcatacca gacataatct atgtttctct
     5821 caggggtgga gcttacatgg gcaatgtgat aagcgagtat ttcaagtttg taaaagataa
     5881 tgagcatccc gttttttatg ctgcagtcgt tgcaaggtca tatactggtt ttgatcaaca
     5941 ggaatcaatc aagatcgacg gatggactta taatccggat ttcttgaggc atggcgataa
     6001 ggtgctgctc gtcgatgatg ttttcgactc aggccgcacc atcaactatc tagctagggt
     6061 cataatggag cgaggcttgc caagaagcga tgtgaagata gctgttcacg actataaggt
     6121 aagagagtat gtccctcagc acttgcctat cctcccagat tattggtgta gaaaaatcga
     6181 agtgaagtcc agggaggatg agctttggat acattatatg agccatgagc tagaagggct
     6241 aacccagcag gaaatagcag agcattatct caaagatgat cccagccttg cagagccgat
     6301 gagttttgtt gctaaagaga gcactagttc aactagcgat aaatgagtcg ctaacaatta
     6361 aattactgta ccatcaggga ctatttgatt ctttggaatg acgataattc cgtctactat
     6421 ataataagtt ctatattcac cttcttggcg tgcaagagga tctattccaa tacggcagtt
     6481 cgagccaata cgagcattct tatctattat tgcgtggcgt atgatagagc cgcctccgat
     6541 acctatatta gggatattct ttctgcgatt ttcctctttt tcttctgcat tctcgtagta
     6601 gtctgagccc atgcacacaa ccccgtcgag gctcgccccc gattcgataa cagtgcgaat
     6661 gcccacaatt gaacgagtaa tcgacgcgtt agttattatg caaccatcgc tggccagagc
     6721 ctgattcaaa gtgcaatagt tgagtttaga agcaggcagg ttacgcgcat gagtatatat
     6781 tggcgattgc tcatcataga aattgaattt cggatttata tctgtaagcc cgatattagc
     6841 ttcatagaag ctacgaactg ttccaatatc ttcccaatag ccatcgaaga catatgtatt
     6901 gactttttct tttgaaacca caaatggaat aatctccttg ccaaaatcat tcgcgtccag
     6961 ctgcatggtc tcttctatca gaggcgcatc gaatatatat atccccatgc ttgccagata
     7021 tggtttatta gggctggctc cttcaggtct aaggcttttt ggtacctcat attcatggat
     7081 atccttagtg gggccgggtt tttccagaaa actatcaatg ctcctatttt cttttatctt
     7141 aataatgcca agagaggtgg ctgtatctcg atcaaccagg gtccccgcta ccgtaaggcg
     7201 actgccggac tcgacatgtt gtttgaacat ctctcctaaa tccatacggt aaagctgatc
     7261 acccgagacg attagatagt gagtcggctg gtgaggccgg aagtgaataa ggttctttct
     7321 aaccgcatca gcagttcctt cataccatcc agaatgcaaa tgagtctgct cagcagccag
     7381 aatctcaaca aaaccctttg agaagcggtc aaaattgtag gaacgatata tatgaagatg
     7441 aagggatgcg gagttgaatt gagtgagaat atagatcttc ctaaaatccg aattgatgca
     7501 gttggataag ggtatatcga caattcggta tttccctcca aatggaaccg caggcttagc
     7561 tctctccttt gtgaggggaa atagacgagt tcccttgccg ccccctagca cgatagaaag
     7621 aatttctgcc atatatgctc ctttcttgtc agctgccgat ttaccttttt tattttgatg
     7681 attacagtat aacttatgaa ttctgaatcc gcaaatgcaa tttatagtga attcttatcg
     7741 tggctggcag aaaggaatga gattgcttgg tcatccatac tgccgccaaa aagtgccgat
     7801 tgcatgccat ttccagaaga tttatctcct atactaagcg aagcgctcca tagaagaggt
     7861 atagatagtc tctacacaca tcaacgaaag tcctatgatt tagttcgtag tggacacgat
     7921 gtagttatag taacaccgac agcctccgga aagacgctct gttacaattt gcccatactc
     7981 cagactcttc ttgagatgcc aaaagccaga gcactttatc ttttccctac aaaggcgctc
     8041 gctcaagacc agcagagcgg actcaatgaa gtaatgcttg ccggaagtat cggtttccct
     8101 gtcaatacct acgatgggga tactccgtcg gatattcgct ctagagcaag aacagatgga
     8161 cgtataatca tttccaaccc agatatgctt catgctggca tactacctaa ccatacaaaa
     8221 tgggtcaaat tcttttctaa tctgaagttc atcgttatgg atgagcttca tgcctatcga
     8281 ggtgtctttg gaagccatgt cgcttcggtc ataagacgat tacttcgaat tgcgaaattc
     8341 tatggatctt ccccgacttt catcttttca tcggcgacaa tcggaaatcc acaggaatta
     8401 gctgagcatt atctagagcg gaaagttgag atgatcgaag ataacggagc gggaagcgga
     8461 gaaaaaatcc tagtctgcat caatccccct attgtggacg aagtgcaggg cttaaggcgt
     8521 tcgaatgctc tcgaagctga atcggcaatg ctatggctat tacaaaaggg tgtgcggacg
     8581 atcctctttt ccagatcacg ccttcaagta gagctgcttg cgcattatct aaatcagaaa
     8641 cttgaaaatc catttaaccg caactatggc attgtggtta aaccctatcg atcgggtttg
     8701 ctcccatcgg aacgcaggga aatagaaagg ggcttgagaa atggaactgt tcatggagtt
     8761 gtctcgacaa acgctctgga gcttggcatc gatataggtg ggctcgatgc cgcagtaata
     8821 actggttatc ctggaagcat cgcttcattt tggcaacaag caggtagggc aggtcgaact
     8881 tcgggcatat ctctggctat ttatgtggct tcttcttcac ccctagatca gtattttgta
     8941 gctcatccgg aatattttct ttcaagggga gcggaacaag ctcatatcga tacatataat
     9001 ccttatatat tcaccgatca tctcaagtgt gctgcttttg agctaccctt ttctgaatat
     9061 gaggcatttt ctcccgataa ttctaatcga agagctgccg aactgactat cgaagcgctc
     9121 gaatgtctgg aggaagaagg tatagtcagg catactgaag gcagattctt ctggtccgcg
     9181 gaaggttatc caggggaaaa aatatccctt cgttccgcta cgacggagaa tgtcgttata
     9241 gtagacataa gcaatgggca tcatgatgtt attggggaaa tggatcggcc aagtgcaaaa
     9301 gagttgctct ttgaaaaagc aatatatatg cacctgggca gtcaatacca ggtaaagaag
     9361 cttgatttcg agaagcgcat ttgtcttgtc gaggcaagcg atgctgatta ttggactgat
     9421 tcgattgtaa agcgcgatat cgcattgctt actgaggatt cggttgaatc gtatagccag
     9481 tttcgcctta tacttggaga tattcttgtg cgaagccagg ttgaaaagta taagaaattg
     9541 cgatttaaca cgcatgaaaa tattggatat ggagagatat ggcaagagcc ggaagaaatg
     9601 cagactcgtt ctcttatgat ggtcatctat cctcagagcc agagtgctca atttttaagc
     9661 cagttcgggc aagaatctgc taattcttta ttatatggtg ttgctaattt gatgcgccag
     9721 ctcgccccag ccaaaattct ctgcgatgtc cgcgatatcg gagttgcctc tcgtgcaaga
     9781 gacccatttt atgagagtcc cgttctctat ctatatgata tgtatcccgg tggcacagga
     9841 attgcggagg cgctttgctc gaacatgagt gggattattg gttctacaat cgaaaggatt
     9901 gccagttgta cgtgccttgt tggctgtcct tcctgtatag gtgtagaaaa tgcaaatcca
     9961 gataataaag cagcatccct aaggctatta gagttgctct cgtgctcttg aattatttga
    10021 atcatctcca gctgaaatat atcgagcaaa gtcggccaaa gggcaggcaa caaggagctc
    10081 agtaagtgaa tcttaaagaa cgccttgcat tgattaaaag caacgaaaag aataataaag
    10141 gaaactcagc tccaagacca agaaaaatgc cggataattg gcggaatatc ttgccttatg
    10201 tatggataag agagattcaa gggcctatat tcatcgttcc tcctttcttt tattcaggca
    10261 ttattcgatc agcacggcct tctgagaggt tttctgagca gacttctgag actgaaggag
    10321 acatcgattc tcaaggctcg gcatttcgaa ttcctgccga tagagtgatt tttttcgacc
    10381 ttgagactac gggactctct ggcggtgcag gaacttatgc ctttctttcg acaaccgctc
    10441 gctttgaagg aaggaatcta gttctcaacc aggtatttct ggaagattat ccgggagagc
    10501 gcgattttct ttcaatggtg atttcgcagc tttcggctgg agactggatt gcaagctata
    10561 atggagctac attcgatatt ccgcttctca ggtcgcggtg catactaaat gggattgtta
    10621 tgccgatcag aatgcatgtc gatgttcttc acgactgccg acgattctgg ggcaagattc
    10681 tcgattcttg ttctcttgcc tccatggaaa aattcgttct taaaagcaca agggaatttg
    10741 atattccagg atctgaaatt ccaagagtct ggctcgatta tgtaaaaagc gattttctaa
    10801 gtgagaacca aaaggcgctc atggaactgg tgtggcagca taatattatg gatgtggttt
    10861 cactagccag gctctttcta catatcgaat cattgtatcg cgatccatac agggctgtta
    10921 tcgaggattc agtggatccg ttaagtttag ccaatcgaat ctgtaagcta ggaagactgg
    10981 aggaggctaa atcgctactc ctgatgatct ataggaacaa taaagaacat gatttatcga
    11041 gggaaataat aagagaagta caacgatatc tcgcaaagct agcgaggaaa gacaaggact
    11101 tggatttatt ctcagagcta gtgctttcta tggatagcga attcctatat ggttgtgtgg
    11161 ctaaagcaaa actcttcgaa catacattca aggatgaaaa gactgctcta gtttgggctc
    11221 agaaagctca tgatttggct tgcaattcag taaactcagg cacaatcaaa agaaaggata
    11281 aggaaatggc cgcccagctt tcagtcattg cctctcttga tcatcgtatt gctcggctcg
    11341 agcgcaagat tgcaaataga aagagtatcc cctagctgtc cacaagctgc cattacgccg
    11401 cggccacggc tcatacgtcg aaccgtcaca attccattat ccgaaaggat ccgagaaaat
    11461 tcttcgatct gacgaacact gggtgttttg aaaggcattc catctatagg attccaggca
    11521 attagattta cctgagattt gatcggggta atccaattta caagctcgat tgcatctttt
    11581 tctcccacat tcaggcctcc gaggagaacg acttcaaatg taatacgtgc attagtgtct
    11641 tgctgatatt cgagaagcgc cgatttgaga tcatcgagag gccatttccg attgacaggc
    11701 atgagctctg ttctaaggtc agggttagcg gatgttagag atacagccag acgcacatta
    11761 gggccattct ttgcaaggtc caaaattgca ggaacaatac cactcgttga tatggttatt
    11821 tttctcatcg acaatccgat gcccgctggg tgcgatagaa tagaaatact ttttctaaca
    11881 ttttcgagat tgagaagcgg ctctcccatg cccataaata cgacattcga tataaatcca
    11941 cggcgctccc gaagatgatg aaattgttcc acgatttcgt caggtccgag atttcgaaga
    12001 aatcccaatg tgccggtctt gcagaatgcg catcccatgg cacagccaac ttggctggaa
    12061 aggcaggctg tctttcttcc ttcgatatct tcaagaagca cacactctac agaggctcca
    12121 tcgcggagtc ttatctgtag cttaagagag ccgtctttat cagaaaactc attctgaact
    12181 accgatgagc ggacactacc accaaaattt tgagccaatc tttctcttag agtcactggg
    12241 agatttgtca ttttttcgaa agatggcact cctctagaaa accatgcaaa tgcttgtttt
    12301 gctctataaa gaggctcttc agataagata gcagccagtt cttcctgaga taggccgctc
    12361 ggatagactg cactcagtct atcagcggaa tcgtaattca tgtcttggct gtaagtttga
    12421 acaaagcatc ctgaataacc gggtttttta tacccatctg ctggaattcg gtgagtattt
    12481 cgattgccat ttggcgctga ttgttcaaga gataggtctg agctagctcg acatagatac
    12541 gatagttctt tgggtcgttc tgcaagagcg agcgcaggct ggcaatagct tcatcatact
    12601 tgccttgctg tcgagcaatc accgctagac ctagtactgc ataaatgtcg aactcgatat
    12661 tgagggcatt ttggtagtat tctgtggcct tttcaaaatc acccatattc cgatatgcat
    12721 ccccggctcg ggtcaggata actttgttct ttggatctct ttccaggatc ttgttccaat
    12781 agagcagtga catattttgt ttgccgactc ctctatagca atccgctatg ccaaaaagag
    12841 cgtagaagtt atccggctct ttttgcaggg ctttttcgaa aatcgggata cctttgtcga
    12901 aatgcttaag cttgcgataa caattaccaa tcgcagtcaa aactcgaatg tcaacgttat
    12961 cgccttccaa ttcgaccatg cgctgccagt atgagagcgc atctttgtac tccttgaaat
    13021 catagtgcag gtgcccaagg cctataaggg cataatgatt atctggattg agttcgagaa
    13081 cgcgatgata aattgatttg gatttccgga agtcgcgaac cttgcgatat gcatcagcaa
    13141 cgcgtgtcaa tacggtgata ttcgtgttgt catggagaag gtattgttcc catatttcaa
    13201 tggccttttg gaattggttg agagccttgt agcagtccgc aagtccgaat aatgcatagc
    13261 tattacctgg atgaaaaacc aagcaacgtc tataatagtc tgcggcctct ttgaagcgct
    13321 cttgctttcg agcggcatct cccaaaccga caagggcata gttattttct ttatcttttt
    13381 caagaatttg aacaaagcat tcaaccgctt catcgaaacg attttccttt aggagctgat
    13441 agcccttttt agagatctcc gcaacctctg tgttctctaa ttggatttca ttaggaattt
    13501 cgaaaaaatc atcattgtag ggcaaagatg gcatatcact catggctttt tacctcttgg
    13561 cggaaagtat ttttaaatat ggctccttgt ggcctaacta tttattacct aagtctttcc
    13621 gtcattcttt tactcttttt cggtttttat tatacctttt agttcatatc aatgcaatat
    13681 tttcagttg
//
LOCUS       DAOMZL010000048        12988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_4437_length_12988_cov_19.461455, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000048 DAOMZL010000000
VERSION     DAOMZL010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 12988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12988
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4437_length_12988_cov_19.461455"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..557)
                     /locus_tag="PLQ30_05275"
     CDS             complement(129..557)
                     /locus_tag="PLQ30_05275"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPW01890.1"
                     /translation="MQLAELCPGQRFKVTNVAVGGEIGKRLADMGLVSGAEGEVVRAA
                     FFRGPIQIRLGDYDLIMRRYEARLVDVELLPEAAPQVAGRPPKKALSTMYVDLRPRER
                     RRSHGATTTPSTHGASGGHGIQDAHGKAHGAHDKDPSCLD"
     gene            complement(559..1002)
                     /locus_tag="PLQ30_05280"
     CDS             complement(559..1002)
                     /locus_tag="PLQ30_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPW01891.1"
                     /translation="MNELDRFETFLRGRGQKITKPRRIILEAFLNTEGHLSTEDILHE
                     AKKRDPGIGQATVFRTIRLIEDAGLAREAVQEDGARTFEHLADHPHHDHLLCTACGKV
                     IEFLSPAIEKEQQKIFAKYGFKPTGHTMELLGLCPECQAKEKGVQ"
     gene            1182..1700
                     /locus_tag="PLQ30_05285"
     CDS             1182..1700
                     /locus_tag="PLQ30_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HPW01892.1"
                     /translation="MGKLYYSYQMIHKLAQSIAEQVMNSGFSPDIIVAIGTGGFIPAR
                     ILRTYLGKPILALGIAYYDLNDKPMDYPRKVQWIEEAEKQLAGKRILLVDEVDDSRLT
                     LEYCTKELLAYRPAALAVSVLHNKQKEKRGAFPPGVQYVFVGENLGDDWIVYPWDARD
                     IDEHDKNAALEH"
     gene            1745..3238
                     /locus_tag="PLQ30_05290"
     CDS             1745..3238
                     /locus_tag="PLQ30_05290"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPW01893.1"
                     /translation="MRHKRSWFCILLSLFLMFLTLDTLVAQQAQAVRNATLENGLEVF
                     VVENHVVPLVTVCIAFRGGAIAHSPETAGLFHLYEHMMFNGNDKYPTKDAFSAALNRM
                     GTTSWNGATGREYINYFISVPSEKLTDAIDFWAHAVINPTLDARVLENEKQVVLNEIR
                     GYHSDPSQIALNGLESRMFRDFPWRKNIDGPESNITRANVTALRDIQKTYYVPSNMAL
                     LIGGDTSLEEVVKLANESFGSWARAPEPLLAEPPHGPIPDGIRLVTSEDQFYRGIAQV
                     EFRWRGPDVTRQTYDTYISDVLLFLLSSPSGPFKSEIMRKVYGLYDAEYIDFVYPTSR
                     DGGNYIFATLMLVQRPAEEEPVLQRVENLKSVVLDEFALIAKDPSAYFGGDEALEEAK
                     TKLVDQNIYALESSGSFVTDTLTFWWSTAGTEYFFDYEENCRKVSWEDISSLIERYLA
                     GESNPNVATLVRLRTSTLNSDPRMDAKISELGYSRLTAQNAFWWQKQ"
     gene            3249..4706
                     /locus_tag="PLQ30_05295"
     CDS             3249..4706
                     /locus_tag="PLQ30_05295"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPW01894.1"
                     /translation="MRHFNNDVSRMFVVVVLMVSVAIGACAHTPYVRENAPTYMKTVL
                     SNGIPLYMKVQRANQVFHVSLVLNGASLVTPPEQAGWEKIALATMARSSKNYPYEKAA
                     SILDRRSSAITSAVQFEYSTFSLTTLDKYSDELLALWSDMLVNPSFDQSDFDREKENA
                     LLALQAIGQNPWTMTQKIMNEAFFKGHPYGVNPEGTEESIAPMMAGDARDWYKEHFSA
                     DRIFLVAIGDFNPAELAYKLEKLLGDIPDLNLGPISKPQEFRIGLSGFGSSSESGLPS
                     GSLITQAHDQSKGIIYLRGDFAAPAPGSDDYFATTLATRIFSDLLFAIVRDKYGAVYT
                     PSAAIRSYTANYGSIMIYKSDAPEKIKQYIDEAAALMAQGRVVSVDPTKTEADGYMAL
                     SDALDTYKQLYINNYFEAVRTNAAIASMMIRSIKLTGDPADWLKDQVRIEMLDASAIQ
                     NAFEKYVINGTFLWVAVGDQALLDRLDRRAFESIR"
     gene            complement(4751..7777)
                     /locus_tag="PLQ30_05300"
     CDS             complement(4751..7777)
                     /locus_tag="PLQ30_05300"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPW01895.1"
                     /translation="MPFVLQKESSVPELDSIIRLYRHERTGARLLSVINKDENKVFGI
                     SFRTPPERNNGVVHIIEHSTLCGSRKYPVKEPFVELMKGSLNTFLNAITFEDKTCYPV
                     ASTNLKDFYNLIDVYLDAVFYPNLTEYTFMQEGWHYEVDPVTRELSYKGVVFNEMKGA
                     YSDPDSLHDHLCKCSLFPDTCYGLDSGGDPLEIPTLSYEEFIAYHKKYYHPSNSFIYF
                     YGDDDPEKRLSFMDEWLSPFEAASIDSLPAIQQTFPEPLLIKRNFQANSKEAAKAYTA
                     VNWMLYEHGDMLLSMQALVLFHILTGTPASPLRKALIESGLGEDIAGFGFYEEMRQTA
                     FSIGLKGVEPHNLAKVEELIFSTLERLACDGIDPDTIAASLNTIEFALRERNTGSFPR
                     GLAIMLDALNEWLYDLDPISALSFAEPLEQTKRAAKENHRLFSELINSLILKNKHRST
                     VRFLPSSGLKNEREEAEKAMLALARASLDDEALRKIEDTAKNLKELQDKPDSPEALAT
                     IPVLSLDDIPGEAPVLPSEPLESATEEFKGSGIYGYYHELSTSGILYLDLGFGFGKLP
                     SRLLPYVSLLGRFLIETGTEKYDFVQLIQRIGMHTGGIRSMVISTTHWAEHRPVPWFF
                     LRAKALPEKVGILSDILLDILTSAQLDNRERVRQIVLEEKAQAEAMLIPASARIVGLR
                     LRSRFNSAYWASERLMGIERLFFLRKLLRNIEEDWPSVLSEIREVHSELIWRDNLLVN
                     ITSDKAILEASAESILRIAQSLPMQAMLAQSLPHRSFPQLDSWVNEASRACYPSFPQG
                     HENSSPKNEDAHSARIPFETIELQTQVNSVGMIMPLKGLDPIAGGALAATKYLDTSYL
                     WEQIRVIGGAYGGFSSLDLASALLMFLSYRDPNFNQTIEAYRKVATFLESCNLPREEI
                     QKSIIGTIGDMDAYQLPDAKGFNALMNILAGYTQETRQHIRDQILAANMDDFRSFGKL
                     LSEALDRSMIVVMTSPEQAEKALSTLPAPIARLSLQ"
     gene            8072..8608
                     /locus_tag="PLQ30_05305"
     CDS             8072..8608
                     /locus_tag="PLQ30_05305"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HPW01896.1"
                     /translation="MKASRKAIRFLMVGAMLMAVTASTFAQGFGGMSNQRAKTPGYGY
                     QSVPPVAPKADPYGNRFGDGSRGFANQEKSFLGNKRSMGAGGYGVYHYFQYQKLSAED
                     RAKVDKIIEDSASKVLPLQNELRAAKLNLDNLLWSANPDKAKIDEAIAKIVDIQKQIQ
                     EIRATNILEINKVFQSTN"
     gene            8784..9338
                     /locus_tag="PLQ30_05310"
     CDS             8784..9338
                     /locus_tag="PLQ30_05310"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HPW01897.1"
                     /translation="MKTNQARLSLFLILIVGIAFLGIFPVAAQAGYGKGMGNQGVMGP
                     TVVREWFRSLNLTDQDLDKLEKILQSRELELVKAQNEIRILQTQIANILVDPDPDLKA
                     IDEAISKSLQYEKIVRMIQIDRQVAIRKVFGEDRWQTILFLVHEARMSEKAGRFANSF
                     STKGLSAEEANRYGRLLGMLRRIM"
     gene            9412..10059
                     /locus_tag="PLQ30_05315"
     CDS             9412..10059
                     /locus_tag="PLQ30_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPW01898.1"
                     /translation="MSSAKKDRVRIEQDKAEAPSLALSSEEETLDFELAARAAKGDKG
                     AFERLIWRWWDRIRGYCASFVGFDEELSEEAVQESLIRIYKALPKWRKESLLGGYIYG
                     ICRTSCADVMRVNARHHARNLSVEEFDSLSMESPHGLGEEDMLRKEASQMLELALKML
                     EPEDRSMLYLHEVDGMGLSELGRMYNLPIGTVKSRLFRARDKMSAMLKEMGYELS"
     gene            10046..10432
                     /locus_tag="PLQ30_05320"
     CDS             10046..10432
                     /locus_tag="PLQ30_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01899.1"
                     /translation="MSSAERNKIDLRADLKDTAMRKAPAFPDFAQVYKKAEEQPRKRQ
                     KILISTAIAITALVASFFVGGLWESRSSADVALIDSWSVTPAPIAIGISALEDEDDQI
                     ILTSSKNSTLNLYIQGLWESTSTNGL"
     gene            complement(10486..11943)
                     /gene="proS"
                     /locus_tag="PLQ30_05325"
     CDS             complement(10486..11943)
                     /gene="proS"
                     /locus_tag="PLQ30_05325"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011601003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPW01900.1"
                     /translation="MPEKITPREADYSQWYIDIVLNAKLADYSPVKGSMVIRPRGYAI
                     WEKIRNEFDRRFKETGHENAYFPLLIPMSFLKKEAEHVKGFAPELAVVTHGGGEELEE
                     PYCIRPTSETIIWAMYRNWIQSWRDLPLLINQWANVMRWEKRTRLFLRTSEFLWQEGH
                     TAHETEKEARQETLRILEIYRDVVESCLAVPVVTGMKSESEKFAGAVETYTIEAMMQD
                     KKALQAGTSHFLGQNFARAFDVQFQNRQGQLDYVWATSWGVSTRLIGAVIMTHSDDKG
                     LVLPPALSPEDAVIIPIYRNDSKAAVIAYAEKLEADLKAAGFRVVLDIDDSYSPGWKF
                     TEWELRGTPVRIEIGPRDIKSNQVVMVRRDTSEKLTTSNTEAASKLRELLKFIQDSLF
                     ERARSFRQENTKPISDLGALIEFFGTETAGTEGAPGGFAESLWCGSPTCEASIKEKTK
                     ATIRCLPLDRQDQIEGPCAICGAPAKYLAIFARNY"
     gene            12034..12861
                     /locus_tag="PLQ30_05330"
     CDS             12034..12861
                     /locus_tag="PLQ30_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01901.1"
                     /translation="MTRRNFRFTYWLFSSISLVILVTSIVLTVRIGNDKRLPFFLADL
                     NLYTEYFFLDSPIGMQLAGALECCFLVFAATFMLIAVLFTFQKTVSAEIYLIALWIFL
                     VEAEALRLPMLIIAQRSTHTGSLISLTRLIYGARFSGLISLFISGLYAVGIGKERPQT
                     GCLLALLLGSVFAMLLPISTDRFQSSFMLQAGYSELTIIVTASLIVLNAIDYFVAARQ
                     KEDKSYVFVGITLTVAAASWVWLWDTRDFKWGLIVVIAFGLCIFASIKILHYIYIWK"
BASE COUNT         3409 a         2852 c         3232 g         3495 t
ORIGIN      
        1 agtttcctac cttgtggtgt gcgcctgtta tagcattgaa tacactggtc tttccagaat
       61 ttggattgcc tgcaagcgca atggtgagaa tgactctatc ctgcatggat tactcctgaa
      121 aaagtctttt aatcgaggca gctaggatcc ttgtcatggg cgccatgagc ttttccatga
      181 gcatcttgga tgccatggcc gccagaagcg ccatgcgtgc taggagtggt agtggcgcca
      241 tgagaacgcc ttcgttcgcg agggcgcaaa tcgacataca ttgtggatag tgctttctta
      301 ggcggcctgc ctgccacctg tggcgcagct tctggaagaa gttcaacgtc gaccaggcga
      361 gcttcatatc ggcgcattat aagatcgtaa tctccaagcc ttatctgtat aggaccgcgg
      421 aagaatgctg cacgcaccac ctctccctcc gcacctgaga cgaggcccat atccgcaagc
      481 cgttttccga tctctcctcc taccgcaacg ttcgtgactt tgaatctctg acccgggcaa
      541 agttcggcaa gctgcatatc attggactcc tttttctttt gcctggcact cagggcagag
      601 gccgagcaat tccatagtat gacctgtggg cttaaatcca tattttgcaa aaatcttctg
      661 ttgttctttc tctatggcgg ggctcaagaa ttcgatcacc ttgccacaag cagtgcagag
      721 cagatggtcg tgatgagggt gatcggcaag atgctcgaat gtacgagcgc catcttcctg
      781 aactgcttcc cgagctagac ccgcatcttc tattaggcga atggttctga acaccgtagc
      841 ctgacctatg cctggatcgc gtttttttgc ttcatgtaga atgtcttcag tgcttagatg
      901 gccttcggtg ttgagaaatg cttcgagaat tattctgcgc ggtttcgtga ttttttgacc
      961 gcgcccacgc agaaaagttt cgaatctatc gagttcattc atttcgattg ccttatccaa
     1021 tatcataaat gaaaagagga aacgaaaagc gataagcagc gaaaaaaata atcattctat
     1081 cgctcttcga gaatgataat cattctcagt taaaagatca agatgagttg tcactcaggc
     1141 gatctcttgt ctatgttatg ccattaccgt agaatcgtta tatgggaaag ctctattata
     1201 gctatcagat gattcataag ctggcgcagt ccattgcgga gcaggtcatg aatagcggct
     1261 tttctcctga tatcattgta gctatcggta ccggcggttt tattcctgcg cgcattctaa
     1321 ggacctatct tggcaagccg atacttgctc tcgggatagc ctactatgac ttgaatgata
     1381 agccaatgga ttatcctcgc aaggtacaat ggatcgaaga agccgaaaag caattggcag
     1441 gcaagagaat tctattagtg gacgaagtgg atgattctcg gctaactcta gaatactgca
     1501 ctaaagagct actcgcatat agacctgcag cgctcgcggt ctcggttctg cacaacaaac
     1561 aaaaagagaa acgcggcgca tttcctcctg gtgttcagta cgtctttgtt ggagaaaacc
     1621 ttggagacga ttggattgtg tatccttggg atgctcgaga catcgatgag cacgataaga
     1681 atgctgcgct ggaacattag cttaaaaact gggatattag ctcagaaagg agagtaaaaa
     1741 gccaatgaga cacaaaaggt cttggttttg catattgttg tctctttttt tgatgtttct
     1801 gacactcgat acccttgttg cccaacaagc acaagccgtc agaaatgcca cgctcgagaa
     1861 cggacttgag gtctttgttg tggaaaatca tgtagtgccc cttgttacgg tgtgtattgc
     1921 ctttcgtgga ggcgctattg ctcatagtcc ggaaactgcc gggcttttcc acttgtatga
     1981 acatatgatg ttcaatggca acgataaata cccaaccaag gacgccttct cagcagcgct
     2041 aaatcgtatg ggcacaacaa gttggaatgg agcgacgggc agagaataca taaactattt
     2101 tatcagcgtg ccttcggaaa aactcacgga tgctattgac ttttgggcac acgccgttat
     2161 taatccaacc ctggatgcca gagtcctgga gaatgaaaag caagttgtac ttaatgaaat
     2221 ccgaggatac catagcgacc cgtcgcagat cgccttaaat ggcttagaat cgcgcatgtt
     2281 cagagatttt ccgtggcgaa agaatatcga tggtcctgag tcgaatataa caagagcaaa
     2341 cgtgacggcg cttcgagata ttcaaaaaac atactatgtg ccttctaata tggcgctcct
     2401 cataggaggg gataccagtc tagaggaagt ggtaaagcta gccaatgaat cctttggaag
     2461 ttgggcaaga gctccagagc ctttgttagc cgagccgccg catggaccta tcccggatgg
     2521 tattcgtctt gtaacttcgg aggatcagtt ctatcggggc attgcccaag tggaattccg
     2581 gtggagagga ccggatgtca cgcgccagac ctacgacacg tacatatcgg atgtgcttct
     2641 gttcttattg agctcaccat ccggaccttt caaatcggaa ataatgcgga aagtctatgg
     2701 gctctatgat gctgaatata tagattttgt ttatccgact tcgcgcgatg ggggaaatta
     2761 tatttttgcc actctcatgc tagttcagag gcctgcggaa gaagaaccgg tacttcagcg
     2821 agtggagaat cttaagtctg tagtcctcga tgaattcgca ctcatagcca aggatccatc
     2881 agcttatttt ggaggcgatg aagcgctcga ggaggccaag acaaagctgg tagaccagaa
     2941 tatctacgca ctcgaatcat cgggatcatt cgttaccgat acattgacat tctggtggtc
     3001 taccgcaggc acggaatatt ttttcgatta cgaggaaaac tgccgtaagg tgagctggga
     3061 ggatatttct agcctaatag agagatattt agcaggagag tcgaatccta acgtagctac
     3121 gctcgtgcgt ctgcgcacaa gcaccttgaa ttcggatccg cggatggatg cgaagatatc
     3181 cgagctaggc tattcgcgct tgactgccca gaatgccttc tggtggcaga aacaatgagg
     3241 gggcaggcat gaggcatttt aacaatgatg tttccaggat gtttgttgtt gtcgtactca
     3301 tggtatcagt ggcaattgga gcttgcgcgc atactcccta tgttcgcgaa aacgctccta
     3361 cgtacatgaa gacagtgctt tccaacggta ttccgctata tatgaaagtg cagcgcgcaa
     3421 atcaggtctt tcacgtttcg ctcgtattga atggcgcttc ccttgttacg cctccggagc
     3481 aggcgggctg ggagaaaatt gctctggcaa ccatggcgag gagttcgaag aattaccctt
     3541 acgagaaggc cgcttcgatc ctcgatagga gatcgtcggc cattacaagt gccgtacaat
     3601 tcgaatattc aactttcagc cttacaacac ttgacaagta tagcgatgaa cttcttgcgc
     3661 tctggagcga tatgctcgta aatccttctt tcgatcagtc cgattttgac agagaaaaag
     3721 agaatgcctt gcttgcgctg caggcaatag gacagaaccc ttggacaatg acacagaaaa
     3781 taatgaacga ggcttttttc aaggggcatc catatggagt caaccccgaa ggcaccgaag
     3841 aaagtattgc gccaatgatg gctggagatg ctcgagattg gtacaaggaa catttttctg
     3901 cggataggat ttttcttgtt gctataggag atttcaatcc agccgagctt gcttacaagc
     3961 tcgaaaagct tcttggtgac attcctgact tgaatcttgg accgatatca aagccgcagg
     4021 agtttcgaat tgggctttcg ggattcggat cttcatcgga atcaggattg ccatcggggt
     4081 ctctcattac gcaagcccac gatcaatcga aggggataat ttaccttagg ggagattttg
     4141 cagcgccagc acccggaagc gatgattatt ttgcgacaac cctggcaaca cgtatttttt
     4201 cggatttgct tttcgcgatt gtgcgcgata aatacggagc agtctacact ccttcggctg
     4261 ccattaggtc ctacacagca aattatggct ccataatgat ttataaatca gatgcgccgg
     4321 aaaaaataaa gcaatacata gacgaagccg ctgctctcat ggcgcaggga agagtggtgt
     4381 cggtggatcc gaccaagaca gaggccgacg gctatatggc gctctcagat gcgctcgata
     4441 cctataagca gctctatata aataattatt tcgaagctgt tcgaaccaat gctgctatcg
     4501 cctcaatgat gattcgctcg ataaaactga caggagaccc agctgattgg cttaaagacc
     4561 aggttcgcat agaaatgcta gatgcctcgg cgattcagaa cgccttcgag aaatatgtga
     4621 ttaacggcac tttcctatgg gtagcagttg gagatcaggc tttgctcgat aggcttgacc
     4681 ggagagcttt cgaatctatt cgctgattat gttcagtcag ctgggctggt gggtgctgtc
     4741 cgtggcgcta tcactgcaga gaaagccgtg caatcggagc cggcagcgtt gaaagcgcct
     4801 tctccgcttg ctctggtgag gtcattacca ctatcatcga acgatcgaga gcctccgaaa
     4861 ggagttttcc gaagctgcgg aaatcgtcca tgttcgctgc aagaatctgg tcgcggatat
     4921 gttgacgcgt ctcctgagta tacccggcca ggatattcat aagggcgtta aatcctttgg
     4981 cgtctggcag ttgataggca tccatatctc caatcgtacc aatgatcgat ttttggatct
     5041 cttctcttgg caaattacag gattcaagga aagtcgctac ctttcggtag gcttctattg
     5101 tctgattgaa atttgggtcg cgataagaaa ggaacataag caaagcggag gcgagatcga
     5161 ggctggaaaa ccctccatac gctccaccaa taactcttat ttgctcccag agataggaag
     5221 tatccaagta ttttgttgcc gcaagagctc caccggctat agggtctagc ccctttaggg
     5281 gcataatcat acctacgcta ttaacctggg tctgcagctc tatcgtttcg aaaggaatgc
     5341 gagcgctgtg ggcgtcttcg tttttcggag aagagttctc atgcccttga gggaaggaag
     5401 ggtaacaagc gcgcgatgct tcgtttaccc atgaatcgag ttgtggaaaa gacctatggg
     5461 gtaaagattg agcgagcatc gcttgcatag gcagagactg cgcaatacgg agaatggatt
     5521 ccgcggaagc ctcgagaatg gctttatccg aggtgatatt gacgagcaga ttgtcgcgcc
     5581 atatgagctc gctatgcacc tcgcgtattt ctgacaacac cgaaggccaa tcttcttcaa
     5641 tgttacgcaa gagcttgcga aggaagaaga ggcgttctat gcccataagc ctttccgaag
     5701 cccagtatgc agaattgaat cgagagcgaa gcctcagacc gacgattcga gcagaagcag
     5761 gaatcagcat tgcctctgct tgtgcttttt cctcaaggac aatttggcgg actcgttcgc
     5821 ggttatctag ttgcgcgctg gtcagaatat ccaaaagtat gtcgctaaga atgccgactt
     5881 tttcgggcag ggctttagcc cggaggaaaa accaaggcac cggcctgtgt tcagcccaat
     5941 gcgttgtaga tataaccatg cttctaattc cgccagtatg catgccaatg cgctgaataa
     6001 gctggacgaa atcatatttt tctgtgcctg tctcaattaa gaaacgaccc agaagactga
     6061 catagggcaa aagcctagaa ggcaactttc caaaaccgaa gcctagatcc aagtaaagaa
     6121 tgccgcttgt cgaaagctcg tgatagtagc catagattcc agaccctttg aattcttccg
     6181 ttgctgattc gagaggctct gaaggcagta caggggcttc tcctggaata tcgtccagag
     6241 agagcacagg aatagttgca agcgcttcag ggctatcggg cttgtcttga agttccttga
     6301 ggtttttggc agtatcttcg atttttctaa gtgcttcgtc atctaagctg gctcgggcta
     6361 gagcaagcat ggctttttca gcctcttcgc gttcgttttt taggccggat gagggcagaa
     6421 atctgaccgt gcttcggtgt ttattcttta agattagcga atttatgagc tcgctgaata
     6481 gtctatggtt ttctttggca gctcttttgg tttgttcaag cggttcggca aaagaaagcg
     6541 ctgatattgg atcgaggtcg tataaccatt catttagagc atccagcatg atggcaaggc
     6601 cacggggaaa agacccggtg ttgcgttcac gcaaggcgaa ttctatggtg ttgagcgaag
     6661 ccgcgatagt gtcgggatcg atgccatcgc aagcgaggcg ctcaagtgtt gagaaaatga
     6721 gctcttcgac ctttgcaaga ttgtgaggtt caacaccttt aagccctatg gagaaagccg
     6781 tctggcgcat ctcttcgtag aagccaaaac ccgcaatatc ctctccgagc ccggattcta
     6841 tcaatgcctt tcgtagagga gaggctggcg taccagtcaa gatatggaag agaacgaggg
     6901 cttgcatcga aaggagcata tccccatgtt catatagcat ccagttgacc gctgtataag
     6961 ctttagccgc ttcttttgaa ttggcctgaa aattgcgttt gatgagtagc ggctccggaa
     7021 aagtctgttg gatagctgga agtgaatcaa tggaagcggc ttcaaatggc gatagccatt
     7081 catccataaa ggaaaggcgc ttttccgggt catcatcgcc atagaaataa ataaaagaat
     7141 tcgaaggatg ataatatttc ttgtgatatg cgatgaattc ttcgtagcta agagtgggaa
     7201 tctccagagg gtcgccaccg gaatcgagtc catagcaagt atcgggaaac aggctgcatt
     7261 tgcataggtg atcgtggagg ctatctgggt ctgaataggc gcctttcatt tcattgaata
     7321 ctacaccctt atagctgagc tctctggtaa caggatctac ttcgtagtgc cagccctcct
     7381 gcatgaaagt atattcggtc aaattcgggt agaataccgc atctaagtag acatctatga
     7441 gattatagaa atccttgaga ttggtgcttg ccaccgggta gcaagtctta tcttcgaacg
     7501 ttattgcgtt gaggaaagta tttagtgagc ctttcatcag ctcgacaaaa ggttccttta
     7561 ccggatattt acgggaaccg caaagagtag aatgctcgat aatgtggact acgccattgt
     7621 ttcgttcagg tggagttctg aaacttatcc cgaagacctt gttttcatcc ttgttgatga
     7681 cagatagaag tcgggctccc gttcgttcgt ggcggtatag ccgaatgata gaatcgagtt
     7741 caggcaccga ggattctttc tgaagcacaa atggcatgcg atactcctct ggaaaatgat
     7801 tattgaagag agtatagaga agcaaaaagc gcaaggttcc gtctctcttt cctcatagct
     7861 taatgcaatg cgtcataata gaaaagtgtc tgcagcataa gaaggcttta acagataaca
     7921 aagttagatg aggatgacca gcaaaaaata atagttatat cctgtcgaga tttattcgca
     7981 aaatcacgtc aaattatgat ttttcatgca accgattttt cctttcttac gacattatag
     8041 tgaacgcaag aaccttcaag ggaggaagga aatgaaagca tcgagaaagg caatcaggtt
     8101 tcttatggta ggtgccatgc tcatggcagt aactgcgagt acttttgctc aaggttttgg
     8161 tggaatgtca aatcagcgag caaagactcc gggctatgga tatcaaagcg tgcctccagt
     8221 agctccaaaa gcagatcctt atggcaacag atttggcgat ggatcaagag gattcgcaaa
     8281 tcaagagaag agttttttgg gcaacaagcg atcgatgggt gctggtggct atggggtata
     8341 tcactatttt cagtaccaga agctatcggc cgaagacaga gccaaggtag ataagatcat
     8401 cgaggatagt gcctccaagg ttcttcctct ccaaaatgaa cttcgcgctg caaagctcaa
     8461 tttggacaat cttctttgga gcgcgaatcc cgacaaggcc aaaatagatg aagccatagc
     8521 caagattgtg gatattcaaa agcaaataca agagattcgt gcaactaaca ttctagagat
     8581 aaataaggta tttcaatcaa ctaactagtt gaaaaacagc tcagcctaga ggctgactga
     8641 cagactaagc gagtcggtcc atactaagga aatgttgctg caaatgggat accttgtaaa
     8701 aggccacgtg gctatcgacg ccgcgtggct cattatacag aacgatatat aagatatata
     8761 attggaggtg gcggatgatt ccaatgaaaa caaatcaggc aaggctatcg ctttttctca
     8821 tcttaattgt cggaattgcg tttctcggta ttttccctgt ggctgcccaa gctggttatg
     8881 gaaaaggaat gggaaaccag ggtgtcatgg gccctacagt ggttcgcgaa tggttccgca
     8941 gcttgaatct tacagaccaa gacctcgata agctcgaaaa gattctacaa tccagggagc
     9001 tcgagcttgt caaagctcag aacgagatca gaatacttca gacacagata gcaaatatct
     9061 tagtcgaccc agatccagat ctaaaagcga tagatgaagc gatctcgaaa agccttcagt
     9121 atgagaagat cgtaagaatg atacagatcg atcggcaagt cgctatcaga aaagtatttg
     9181 gcgaagatcg ctggcagacc attttatttc ttgtccatga agctcggatg tcggaaaaag
     9241 cagggagatt tgcgaattcc tttagtacta aagggcttag tgctgaagaa gcaaatcgat
     9301 acggccgtct tcttggcatg cttcgcagaa tcatgtgatg aaaaatatga aaaagcatta
     9361 tattaggaaa agcactatat tatgaataga gacaatagaa tggtttggac tatgagctcc
     9421 gcaaaaaaag atagggtccg aattgagcaa gacaaggccg aagctccgtc tcttgctctt
     9481 tcgtccgaag aggaaacgct ggatttcgag cttgcagcaa gagcagcaaa gggagataag
     9541 ggagcatttg agcgcttgat atggcgttgg tgggaccgca ttcgtggcta ttgcgcgagc
     9601 tttgtcggat tcgatgaaga gctttccgag gaagcagtgc aagagtcgct tattcgtatc
     9661 tataaggcct tgcccaaatg gcgcaaagag agtctcttag gcggctatat ctatggtatt
     9721 tgccgaacaa gctgcgccga tgtcatgcgt gtcaatgcgc gtcatcacgc tcggaatctt
     9781 tctgtggaag agttcgattc tctttctatg gagagtccgc atggattggg cgaggaggac
     9841 atgctaagaa aagaggccag tcagatgttg gaacttgcct tgaaaatgtt agagcccgag
     9901 gatcgctcta tgctctatct acatgaggtt gatggcatgg ggctctcaga gcttgggcgc
     9961 atgtacaatc ttcctatagg gacggtcaag tcaaggcttt tccgtgcacg agacaagatg
    10021 agcgcgatgc tcaaggagat gggatatgag ctcagctgaa aggaataaaa tcgatctaag
    10081 ggctgatctc aaagatacag ccatgaggaa agcgcctgct tttcctgatt ttgcgcaggt
    10141 ttataaaaag gcggaagagc aacctcggaa aaggcaaaag atattgattt cgacagctat
    10201 tgcgataacc gcccttgttg ccagcttttt tgtagggggc ttatgggaaa gccgttcttc
    10261 tgctgatgtt gcattgattg actcatggtc tgtaacacca gccccgatag ctattggcat
    10321 aagcgcattg gaagacgaag acgatcagat catccttact tcttcgaaga attccacact
    10381 gaatctctat attcagggtc tgtgggagag cacatcgaca aacgggctat gaaggccttt
    10441 gtttgcattt catcatctac atatcccata tacaaatatt aacgctcaat aattgcgcgc
    10501 aaagatagct agatactttg caggcgctcc gcagatcgca caaggtcctt ctatttgatc
    10561 ttgtctatcc agcggcaagc agcgaattgt agctttagtc ttttctttga tagaagcctc
    10621 acaagtggga gaaccacacc agagggattc cgcgaatcca ccgggcgctc cctctgtgcc
    10681 agcggtctct gtgccgaaga attcgatgag ggctccaaga tcggaaatgg gctttgtatt
    10741 ttcttggcgg aaagatcggg ctcgctcaaa cagagagtct tggatgaact taagcaattc
    10801 tcttagtttg cttgcggctt cggtgttgga ggtagtcagt ttttcgcttg tatctcttcg
    10861 caccattaca acttgatttg attttatatc gcgaggtcct atttcgattc gaactggtgt
    10921 accgcgaagt tcccattcag tgaatttcca tccggggcta taggaatcgt cgatatccaa
    10981 cacaactcta aagccagcgg ctttgagatc agcttcgagc ttttctgcat aagcaataac
    11041 ggctgctttc gaatcgttgc gatagatggg aataatgacg gcatcctcag gagatagggc
    11101 gggtggcagc acaagcccct tgtcatcgga gtgtgtcatt atgaccgcac ctatgagtct
    11161 agtggaaacg ccccagctcg tcgcccacac atagtccagc tggccttgcc gattttggaa
    11221 ttgcacatcg aaagctcggg caaaattttg tccgagaaag tgactcgtgc ctgcctgcag
    11281 ggcttttttg tcctgcatca ttgcctcgat tgtgtaggtc tccactgccc ccgcaaattt
    11341 ttcgctctcg ctcttcatcc ccgtgacgac cggtaccgca aggcagctct caacaacatc
    11401 tcggtatatt tctagaatgc gcaaggtctc ctggcgggct tctttttctg tttcatgggc
    11461 agtatggcct tcctgccata ggaattccga ggttcttaga aagagccgcg tgcgcttttc
    11521 ccagcgcatt acattcgccc actgatttat cagcagaggc agatcgcgcc aggactgaat
    11581 ccagtttcga tacatcgccc agataatcgt ttcgcttgtg gggcgtatgc agtatggctc
    11641 ttctagctct tctcctccgc catgggtcac tacggcgagc tcaggagcaa agcctttgac
    11701 atgttctgct tctttcttta gaaagctcat tgggatgagc aaggggaaat aagcattttc
    11761 gtggccggtt tccttgaatc gcctatcgaa ttcgtttcga attttctccc agattgcata
    11821 tcctcgcgga cggatcacca tactgccttt gacaggtgaa taatcggcaa gctttgcatt
    11881 caaaacgata tcgatgtacc actgcgaata atcggcttcg cgcggcgtta ttttttctgg
    11941 catttgcaac acctcttcca gtcgcgagga ttcgtcatac tatagtgaaa agaagtgctt
    12001 caatcaattg ctggaatgaa gtaggatata aatgtgacaa ggcgcaactt tcgctttaca
    12061 tattggcttt tttcaagtat tagcttggtg attcttgtaa cctcaattgt gttgactgtt
    12121 cgcattggaa acgataagag acttcctttt tttcttgctg atttgaatct ttatacggag
    12181 tattttttcc tagattcacc gataggaatg cagttggccg gagctctgga gtgctgtttt
    12241 cttgtatttg cagcgacttt catgctgatt gctgtgcttt ttacattcca aaaaacagtc
    12301 tctgcggaga tatatctgat tgctctttgg atttttttgg tcgaagccga agctctaagg
    12361 ctcccaatgc ttatcatagc gcagcgaagc acacacaccg gttcgttgat ttcgctcacg
    12421 agactgatat atggcgcaag attttcaggg cttatttcgc tctttatttc cggtttatat
    12481 gccgtaggaa tcggaaaaga gaggccgcaa acaggctgtt tattagctct ccttttagga
    12541 tcggtgtttg ctatgctctt gcctatcagc accgatcgct ttcagtcttc gttcatgctt
    12601 caggcagggt atagcgagct cacaattatt gtaactgcct cgcttatagt cttgaatgct
    12661 atagattatt ttgttgcagc aagacaaaaa gaagataaat cttatgtgtt tgtaggaatt
    12721 acgctcacag tggcagccgc ttcttgggta tggctttggg acacaagaga cttcaaatgg
    12781 ggattgatag tggttattgc atttggactc tgcatttttg cgtctataaa aatccttcat
    12841 tatatctata tatggaaata gcatttactg caacgaacct tgtgtatgct ttaggcattc
    12901 tataaggaat tgtctcaagc ataggcatag gcgggcacat actctgagtt tttcttaagg
    12961 ggagtgggga agccccggag ccagagtt
//
LOCUS       DAOMZL010000049        12472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_4697_length_12472_cov_34.167271, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000049 DAOMZL010000000
VERSION     DAOMZL010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 12472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12472
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4697_length_12472_cov_34.167271"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLQ30_05335"
     CDS             complement(<1..432)
                     /locus_tag="PLQ30_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPW01902.1"
                     /translation="MKKMLGTILVAINGSDASICAFKYALALAKAFGSKIVACYVVDT
                     ATIRQLALSRIFVPEESEEYERNLENSGQRYLHFCAELAHQKQLDIETSIRRGSVSGE
                     IVKYSMEIDADIIVLGGNPVDVIYRDAIADSNHEVLKNARCP"
     gene            complement(438..1061)
                     /locus_tag="PLQ30_05340"
     CDS             complement(438..1061)
                     /locus_tag="PLQ30_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPW01903.1"
                     /translation="MPEQMPAPTKTNLIKEKRNLQLALQGYDLLEKKREILVIELMKR
                     MDSLELLEREIADMTEKAYAALRKMLLLVGRERALAISSLPVREITLSASHINVSGMR
                     LPSLDVHASNPTLNYSFMNSFAICDETVIEFTELLDKLSTAAGMRSVVWRLAREVRKT
                     QRRVNALDKMVIPRSQSIVKYIDASLDERERESLFAVKMLKRRQIGQ"
     gene            complement(1065..2528)
                     /locus_tag="PLQ30_05345"
     CDS             complement(1065..2528)
                     /locus_tag="PLQ30_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPW01904.1"
                     /translation="MRGLAYHGIERADGPIVIAERRENIGFSELVRVKDRQGEYRLGR
                     VVELSEKAVAIQLFAENTGISIDDAWVEYLEKPLTFRVGEGIVGRIFNGLGEPIDGYP
                     PILSSDLRDINGLPINPSARVYPRDFIQTGISAIDGMNTLIRGQKLPIFSGNGLPHNR
                     LAAQIVRQAKVLSGESQFAIVFAGMGIKYDVARFFIDSFESSGVLARVVMFLSLADSP
                     SIERLVTPRTALTAAEYLAYERNMHVLVVMTDMTNYCEALREVSAARNEVPSRKGYPG
                     YLYSDLASIYERAGKIEGSEGSITQLPILSMPNDDISHPIPDLTGYITEGQIVLERDL
                     SQRGVYPPIAGLPSLSRLMKDGIGEGMTREDHRELAAQLFSAYAYVHTVRDLAAIIGE
                     EELSDRDKIFLNFGDHFESEFLAQGEFENRSIEQTLDLGWKMLSLLPESELVRIPPQI
                     LAKYLPRDDVGKQNVQNDQYNQKKTHEAAALKEPKEK"
     gene            complement(2525..4336)
                     /locus_tag="PLQ30_05350"
     CDS             complement(2525..4336)
                     /locus_tag="PLQ30_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPW01905.1"
                     /translation="MSSKELGQKKNMNTSIRTGVIRRISGPVVLAQGLEDAGLYDVVK
                     VGESGLIGEIIKLDGDEATIQIYEDNTLMRVGEPVECMHRPLSVALGPGLIGSIYDGI
                     QRPLPVLKGLSGAFLKPGLAGEPLDTEKKWHFLPLLHEGDPIGQGIAYGRIQETSSIE
                     HRLIFDARVPAGIAAYVAPEGDYTVAETLVRSADGASYTSVSNWPVRKARPFREKQMT
                     REPLVTGLRVIDVLFPIAKGGCAAVPGGFGTGKTMTQHAIAKWCDADIIVYIGCGERG
                     NEMTEVLTEFPSLLDPRTGRSLMERTVLIANTSNMPVAAREVSIYTGITIAEYYRDMG
                     YHVAVMADSTSRWAEALRELSGRLEEMPAEEGFPAYLPTRLAEFYERGGMVATLGQKM
                     GSVTIIGAVSPPGGDFSEPVTQHTRRFIRCFWALDRELANARHYPAISWNDSYSEYTD
                     DLIAHWDAIDPSWDRLRIKAMDILKKERRLSDIVRLIGPDALPDEQRLILLTADMIKN
                     GFLQQSSFDKVDMYCAPQKQTLLLQCILTFHELAENAIKNGAPLPKVSALPIREKIVR
                     LKSSLENDKVEEGRVVIQEIQAAFEQLGVTVQGAVLA"
     gene            complement(4373..5113)
                     /locus_tag="PLQ30_05355"
     CDS             complement(4373..5113)
                     /locus_tag="PLQ30_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01906.1"
                     /translation="MEEIRGTEALEREILEDARKRADRIIRKAEENARLLGAQTEQRI
                     KEAKAALLSEYQIKKKTAELEMLSRLPLEKARLDILYRDEIFRKALQEVLASMNPRLF
                     GLWCVKRLAYQVDLVRKSPAKVLVHGLDSETMRDIGALFRQGSSISEDGKNSVMGSDS
                     GQVLGAGQDSGMQQDSELRQDSGQLLRTGPNSDISIEEAPAMKVRGLIVEPMDTSYRI
                     SITENELLEWLLDEKRGELAAALFGSST"
     gene            complement(5136..5483)
                     /locus_tag="PLQ30_05360"
     CDS             complement(5136..5483)
                     /locus_tag="PLQ30_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HPW01907.1"
                     /translation="MAEIFVLAEEELLIAFGMIGIKGAAISNREDAVAAFRSVVQDKR
                     YSREGQTLDLSDCKMLVLSEDVSDMIGEELFEWQLSGAFPLIVEIPPLAGSSAQHTRL
                     VDAVRKAIGIKIQ"
     gene            complement(5555..5863)
                     /locus_tag="PLQ30_05365"
     CDS             complement(5555..5863)
                     /locus_tag="PLQ30_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HPW01908.1"
                     /translation="MKKKLLFGVMAFMASVAVFGQTQAEPTAAVSSSVQMVKYIAAAL
                     AVGIAAMGGAMAVAKIGAAAMGAMAEKPDLSGKALPYVGLAEGICLWGFLVAVLILFL
                     "
     gene            complement(5902..8064)
                     /locus_tag="PLQ30_05370"
     CDS             complement(5902..8064)
                     /locus_tag="PLQ30_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPW01909.1"
                     /translation="MSSVSMRKVELLLLKSDIDAVLKYLATKRCFQVIYPDELERLAR
                     EKRASFRAVDKAESLVSNNAIREASPGEITIGKPAIGEVQFNEAHLENEQIDETQLDG
                     ERLDEAQRKLDFIGQFLGIESPKDIIEDSRLPDEDMLSKLEPLFERCSQLKLDIDEEQ
                     AKVDGLAESIHEAKAFSSLSRPFEEFEKFSYVSIQIGKVAKERIQELSQMLGDRAVII
                     PLDEEGTILAASSRKGRFAFETALSKVGFEKKKPPEGLKGIPSETMSALEGAYQAEKL
                     RLEKLLTEKKELKDKYGDLWRTMVSSIRLKKAIALVESKLEKTRWAYRLSGWVPENMV
                     DKLVKDLLAMLGNRISIRVYDPPEHGVSGSDEKQEAVPVLLKHNPFVSAFQGIVLSYG
                     TPLYGDIDPTPFVAFFFTLLFAIMFGDVGQGMVIAGLGLAMLKAKKGLLASYKKYAHA
                     FIAAGLGSMIMGLLVGSFFTSDTALIPIERVLTGLILGKPADRFLQIMPRDNVGAMFY
                     FFGFTLAVGVIINSTGLVINIINLIRRKEYGEAFFSKTGLAGALLFWWAIGIVVRIIL
                     GSKLGAIDIIGIGLPLLAIIFAEPLKTIIEKGQGRPHTEGASSWLIGGIIELLDIVSY
                     FASNTFSFLRVGAFALAHAVLSFVIFTMGSLVRGKSVSGLAFEILVFLFGNAVIIVLE
                     GLIVTIQVIRLQYYEFFSKFFTRTGEPFKPVSFAGKVE"
     gene            complement(8061..9062)
                     /locus_tag="PLQ30_05375"
     CDS             complement(8061..9062)
                     /locus_tag="PLQ30_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HPW01910.1"
                     /translation="MPETLDIAYLYARVCGAFSNMRLGEKAGELMQNSGGILALWKLL
                     FDEEPPNIPEPRLVFEAERRIIKRAIDDFVRLTEPFSDSNEFIRALISKYEISTIKSM
                     LFRMRSGEPKPEGTTYFSKVIEQALARWPRIADMFSDTPYSWLDSHWLDNIAMAENEL
                     DKQYYLELWKAALGLPRRKVGAILDLIRWEIIYQNIVWAIRVRRYYRMSKEDIADTLV
                     DLPQEDTTSLALETFSYDLERVESFDSWRLKKLLSNQTGPGLDVPALEISALEDLFFM
                     VRRSLHLYPFSYTPIYCYFKLLDYETSLLLAVLESTKLGATSDEKMKYVWIPGGETA"
     gene            complement(9064..9372)
                     /locus_tag="PLQ30_05380"
     CDS             complement(9064..9372)
                     /locus_tag="PLQ30_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01911.1"
                     /translation="MDSTDNIDKLLKIEQQAAQIIQEAEEKASRLLIEAKAQSEIAQN
                     QKITQMRKELEADFASYVDSLNLKSHKEIDEYRLSLNSIPLNQKALLETLEALLQTEN
                     "
     gene            complement(9382..9858)
                     /locus_tag="PLQ30_05385"
     CDS             complement(9382..9858)
                     /locus_tag="PLQ30_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPW01912.1"
                     /translation="MILQNAFKPATIKIELESEYKDELLEELVDILAKDYPKDQLFPR
                     EEALRALRKREEKMSTGIYKGVAVPHATFEGIDKLRGVIGISKRGIDFDSLDGNPVYL
                     VFMLVSPPSQAEAHLEALRQIALLIQDPLLLENLMKATTPEKVYALIREFEDSLNP"
     gene            10204..11061
                     /gene="uppP"
                     /locus_tag="PLQ30_05390"
     CDS             10204..11061
                     /gene="uppP"
                     /locus_tag="PLQ30_05390"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HPW01913.1"
                     /translation="MLIIESIILGIIQGLAEFIPISSSAHLIIVPWLFGWSNPALTSL
                     TFDVALHLGTLLAVIVFFASDWARLISAWFKSVVQFKIGDDPDRRMAWYILLACIPGG
                     VSGLLLESKINEAFHSESIPRSSMLFMAGSIALLALLLWIADKYARHQKNFGQIKARD
                     ALCIGLAQAFAVIPGVSRSGSTITAGLALGLEREAAARFSFLLSAPIIAGAGLKSLYD
                     FSEQIGAGTIAKSELSIFPIGFIVAALSGFFCIKYLLSYLKKHSTEVFVWYRFALAAL
                     VVAVSLIRG"
     gene            11235..>12472
                     /locus_tag="PLQ30_05395"
     CDS             11235..>12472
                     /locus_tag="PLQ30_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HPW01914.1"
                     /translation="MKIVLIGAGSAQFGFGTLGDIFSSSLLKGSHIALVDIDGETLSH
                     VYNAAQAFIDAHGLDFSLSAHTDRREALKAADFVVISIEVGDRFELWDQDWMIPLQYG
                     IPQVYGENGGAGGLFHALRIVPPILDICQDCTELCPNATIFNFSNPMTSITTTVLRKF
                     PQLKFIGLCHEIASLARYIPPMLGVPLDKLELRAAGLNHFSVLVSARYRDTGDDAYPE
                     ILAKAPDFFERMIGYSDILEYIQETGNIPKTEGETDLPNIGRTVSSRHWADRRLFRQI
                     LERFHLLPITSDSHIGEYIPWAHDVADNKGILDFYLLYRSSLSMLEPKIELKVKERLV
                     PIIEGILQDSGYEEAAVNIMNNGLIPSLPQNVTVEVPALVHRKGIEGVAFPNYPRAFA
                     ALLRNYTGVYDLTAEAILHK"
BASE COUNT         3123 a         3262 c         2758 g         3329 t
ORIGIN      
        1 aggacaccga gcatttttta gcacttcgtg attggaatcg gcaatcgcat cgcgatatat
       61 cacatcgacc ggattaccac ctaacactat gatatcagca tcgatctcca tcgaatattt
      121 gactatttct ccagaaacgg aacctctacg gatggatgtc tcgatatcta gctgtttctg
      181 atgagcaagc tctgcacaaa aatggagata gcgctgaccc gaattctcga gattgcgctc
      241 gtattcttcg ctctcctcag gcacgaagat tctggaaagt gcaagctgac gaatggtcgc
      301 cgtgtcgact acatagcagg caacaatctt cgagccaaag gcttttgcga gcgccaaagc
      361 atatttgaaa gcacagatgg aagcatctga accattgatc gctaccaata tggtccctag
      421 cattttcttc atgattctta ctgtcctatt tgccgcctct tgagcatctt tactgcaaac
      481 agcgattcgc gctcccgctc atctagagaa gcatcgatat atttcacgat cgattgcgat
      541 cgaggtatca ccatcttgtc caatgcgttc actctgcgct gagtcttgcg gacctctcgc
      601 gcaaggcgcc atacaaccga cctcatacct gctgctgtcg atagcttgtc aagaagctca
      661 gtaaactcta tcacggtttc gtcacatatg gcaaatgaat tcataaaaga ataattcaag
      721 gtaggattac tagcgtgcac atcgagggaa ggaagccgca tcccacttac attgatatga
      781 gaagcgctga gagtgatttc gcgcaccggc aaggatgaaa ttgcaagcgc tctctctcgg
      841 ccgaccaaaa gaagcatctt ccgcaaggct gcataggcct tttctgtcat gtccgcaatt
      901 tcccgttcca gaagctcgag actatccatg cgcttcatca gttcgatgac aagaatctcc
      961 ctctttttct cgagaagatc ataaccctga agcgcaagct gcagattgcg tttctccttg
     1021 atgagatttg tcttggttgg cgcaggcatt tgttcgggca tgggctattt ctcctttggc
     1081 tcttttagag cagccgcttc atgggttttt ttctgattat attggtcatt ttgaacattc
     1141 tgcttgccca catcatcacg cggcaaatat tttgcaagaa tctgaggcgg aatgcgaact
     1201 agctccgatt ctggaagaag cgagagcatt ttccaaccca gatcgagagt ctgctctatg
     1261 ctgcggttct cgaactcgcc ctgcgctaaa aactcgctct cgaaatgatc accaaaattt
     1321 aggaatatct tatctctgtc cgagagctct tcttctccta ttatggctgc tagatcccga
     1381 actgtatgaa cataggcata ggcagagaaa agctgggcag caagctcgcg gtgatcctct
     1441 cgcgtcatgc cctcccctat gccatccttc attaggcgcg aaaggctggg aagccctgca
     1501 atgggcggat acactccgcg ctgcgaaagg tctcgctcta gcacgatctg accttcggtt
     1561 atatagcccg tcaaatctgg aatggggtgt gatatatcgt cgttgggcat cgaaagaata
     1621 ggaagctgag ttatggatcc ctcgctgcct tcgatttttc ctgctctctc gtagatcgaa
     1681 gccaggtctg agtagagata acctggatag cctttccttg aaggcacctc gttgcgggcc
     1741 gcggagactt ctcgaagagc ctcacagtag tttgtcatgt cggtcatgac tactagaaca
     1801 tgcatattcc tttcgtatgc aaggtactcc gcggcggtca gcgctgtgcg cggcgtgaca
     1861 aggcgctcta tcgatggact gtctgccagc gatagaaaca tcaccactcg agctaagaca
     1921 ccggagcttt cgaaagaatc tataaagaat ctagcaacat cgtattttat tcccataccg
     1981 gcaaatacta tagcaaactg actttctccg ctgagtactt ttgcctgcct cactatctgg
     2041 gcggctagcc tattgtgagg aagaccgttt ccagaaaaaa tcggcaattt ttggccgcga
     2101 atgagggtat tcatgccatc tatcgctgaa attccagtct gaatgaagtc ccttgggtac
     2161 acgcgagcgc tcggattgat aggcaggcca tttatatccc ttaggtctga agacaatata
     2221 ggtggatagc catctatggg ctccccaagg ccattgaaaa tcctgccgac aatgccttcg
     2281 ccaacacgga aggtaagtgg cttttcaaga tactcaaccc aggcgtcatc tatagatatg
     2341 cctgtattct ccgcaaaaag ctgaatggcc actgcctttt ccgataactc cacaacccgg
     2401 ccaagtcgat attctccctg ccgatctttg acccgcacaa gctcggaaaa accgatgttc
     2461 tccctgcgtt cggcgatgac aatgggacca tcagcccgct ctattccatg gtatgcaaga
     2521 cctctcatgc tagaaccgct ccttgcaccg tcacgccaag ctgttcgaag gcagcttgaa
     2581 tttcctggat taccactctg ccttcttcta ccttatcatt ctccagcgaa ctcttgaggc
     2641 gaacaatctt ctcccgtatc ggaagcgcag ataccttcgg aagaggagca ccattcttaa
     2701 ttgcattctc cgcaagctca tggaaagtaa gaatacattg aagtaagaga gtctgctttt
     2761 gaggagcaca atacatatcg accttatcga aactgctctg ctgtaggaat ccatttttga
     2821 tcatgtccgc ggtgaggagt atgagtcgct gctcatccgg tagagcatcg gggccgatga
     2881 ggcgcacgat atccgaaagc cttcgctctt ttttgagaat atccattgcc tttattctaa
     2941 gcctatccca ggatggatcg attgcatccc agtgagctat caaatcatcg gtgtactcgc
     3001 tgtaggaatc gttccaagaa atcgctggat agtgccgcgc atttgcaagc tcacgatcta
     3061 gtgcccagaa acagcgaata aaccgccttg tatgctgtgt aactggttcg gaaaagtcac
     3121 ctccaggtgg agaaaccgct ccgatgatcg tcaccgagcc catcttttgc cctaatgtcg
     3181 cgaccatacc gccgcgctca tagaactcgg ctagccgagt tggaagatag gctggaaaac
     3241 cttcttcagc aggcatttcc tcaagccgac ctgatagctc cctcagcgcc tcggcccagc
     3301 gagatgtcga atccgccatg accgcaacgt ggtatcccat atcgcgatag tattctgcta
     3361 tcgtaattcc tgtataaata gagacttcac gcgcggcaac aggcatatta gatgtattgg
     3421 cgatgagcac cgtgcgctcc atgagcgagc ggccagtacg aggatcgaga agactaggaa
     3481 actcggtcag gacttcggtc atctcgttgc cgcgctcgcc gcacccaatg tacacaataa
     3541 tgtccgcgtc gcaccacttc gcaattgcat gctgcgtcat cgtcttcccc gttccaaaac
     3601 cgcccggaac cgccgcgcac ccgcccttcg caattggaaa gagcacatct atgacccgca
     3661 agcccgttac aagcggctcg cgcgtcatct gcttctccct aaacggacgc gccttacgca
     3721 caggccaatt ggaaaccgaa gtataactgg caccatcggc cgaccttaca agcgtttctg
     3781 caactgtgta atcgccctct ggagccacat aagccgcaat tcccgcagga actctcgcat
     3841 cgaatatgag cctatgctca atagaagaag tctcttgaat ccttccatag gcaatacctt
     3901 gacctatggg atcgccctca tggagaagcg gcagaaaatg ccattttttc tctgtatcga
     3961 gcggctcgcc agcaagccca ggcttaagaa aagcacctga aaggcctttt agcaccggca
     4021 gaggcctctg aattccatca tagatcgaac ctataaggcc aggcccgagc gccacagaaa
     4081 gtggtctatg catgcactca accggctctc ccactcgcat aagtgtgtta tcttcgtaga
     4141 tctgaatcgt ggcctcgtcg ccatcgagct ttatgatctc cccaattagc cccgattctc
     4201 cgacctttac gacatcatag agccctgcgt cttctaggcc ttgagcaagg acaacaggcc
     4261 ccgaaatcct cctaattacc cctgttcgaa tgcttgtatt catattcttt ttttgcccta
     4321 gttctttcga actcatttct ttgcttctat tcccttgcgc caatcgctga attcaagtag
     4381 aactaccaaa gagcgccgct gcaagctcgc ctctcttttc atctaagagc cactccaaaa
     4441 gctcgttttc ggttatggaa atgcgatagc ttgtatccat gggctcgact atcaagccac
     4501 gtaccttcat ggctggagct tcctcgatcg agatgtctga atttggccct gtccgcaata
     4561 gttgcccaga atcttgccgc aattctgaat cttgctgcat tccagaatct tggccggccc
     4621 ccaagacttg ccccgaatca ctccccatta ctgaattttt cccatcctcc gagatactcg
     4681 aaccttgcct aaacaatgct ccgatgtccc tcatcgtctc tgaatctagg ccatgtacaa
     4741 gcacttttgc aggtgactta cgaactaggt caacctgata agcaagcctt ttcacacacc
     4801 acaggccaaa aagacgagga ttcatgcttg caagaacttc ctgtaacgct ttgcgaaaaa
     4861 tctcatcgcg atacaagata tcgagccttg ccttttcgag aggaagacgc gaaagcattt
     4921 cgagttcggc agtcttcttt ttgatttgat attcgctcaa aagagcggct ttagcctctt
     4981 ttattctttg ctcggtctgg gcgccaagaa gcctagcgtt ttcctcggcc ttcctgatta
     5041 tccgatcggc acgcttgcgc gcatcttcga ggatttcccg ttctagggct tctgtgcctc
     5101 tgatttcttc catgcttttt cctaattctg ctttactatt ggattttgat tccgatcgcc
     5161 ttccgcactg catcgacaag acgcgtatgc tgagctgaag aacccgcaag cggcggaatt
     5221 tccacaatca gaggaaaggc tccgctcaac tgccattcaa agagctcttc gccgatcata
     5281 tccgatacat cttcgctcag tacgagcatc ttgcagtctg agaggtcaag agtttggcct
     5341 tcgcgagaat agcgtttatc ctgaacaaca cttctaaatg cagcgacagc atcttctcga
     5401 ttggatatgg ctgccccttt tatgccaatc ataccaaagg ctatgagaag ctcttcttca
     5461 gccagcacga aaatctctgc catagtatcc tctaggataa tgccctatcg tatgcaaaag
     5521 ccttgcctta ggaatcatgc caatccatta tctctcatag gaataaaatg agtacggcaa
     5581 ccagaaatcc ccaaagacaa atgccttctg caaggccgac ataaggcaag gccttacctg
     5641 aaaggtctgg cttttctgcc atggctccca ttgccgctgc gcctatcttt gcaacagcca
     5701 tggctccacc catcgccgct ataccgacgg cgagagcagc ggcaatatac tttaccatct
     5761 gtaccgagga cgataccgca gccgtaggtt cagcttgcgt ctgcccaaag accgctactg
     5821 aagccataaa ggccatgacg ccgaatagca gtttcttctt catacaaaac tccttcttat
     5881 ataatgttat agatcatggc ttcattctac tttacctgca aagctcactg gtttgaaggg
     5941 ctcccctgtc cgagtaaaga acttcgagaa gaactcataa tattgaagcc tgataacttg
     6001 aatagtcaca attagacctt ccagcacgat tatcaccgca ttgccaaaca aaaagactag
     6061 gatttcgaat gccagtccgg aaactgattt acctcgcaca aggctgccca ttgtaaatat
     6121 tacaaaagac agcacggcgt gggctaaggc aaaggctcct accctcaaaa agctaaatgt
     6181 attcgaggca aaatagctga ctatatccaa tagttcgata attccaccta ttagccagct
     6241 cgaagcccct tccgtatgcg gcctcccttg tcccttctcg ataattgttt ttagcggctc
     6301 ggcgaatatg atggccagaa gcggcagccc aatacctata atgtctattg caccaagctt
     6361 gctccccaaa attatcctaa ctacaatgcc gatagcccac caaaacaaga gcgcaccagc
     6421 aagacccgtc ttagaaaaaa aggcttcacc atactctttt ctgcgtatga ggttgattat
     6481 attgataaca agccctgtag agttgattat gactcccacg gccaatgtaa atccaaagaa
     6541 atagaacatg gcgcccacat tgtctctggg cattatctgc aaaaaccgat ccgctggctt
     6601 gcccaagatt aaaccagtca aaaccctctc tatcggaatg agagccgtat cgctagtaaa
     6661 gaaagaacct acaagcagcc ccattatcat cgaaccaagg ccagctgcga taaaagcatg
     6721 tgcatatttc ttgtagctcg ccagaaggcc tttttttgcc ttgagcatcg caagccctag
     6781 acctgcaatc accatgcctt ggcccacatc gccgaacatt atcgcgaaca gcaaagtgaa
     6841 aaagaacgca acgaatgggg tcgggtctat gtctccatat aggggcgtac cataagatag
     6901 gacaatgccc tgaaacgctg atacgaaagg gttatgcttt aagagaaccg gcactgcttc
     6961 ttgcttttcg tcggaaccgg aaacgccatg ttccggcgga tcgtagacgc ggatggaaat
     7021 ccggttccca agcatggcaa gtaggtcctt tactagttta tctaccatgt tttcaggcac
     7081 ccatcctgaa aggcgatagg cccatcgcgt tttttcgagc ttgctctcca ccagggcaat
     7141 agccttcttg agcctaatcg aggacaccat cgttcgccac agatcgccgt acttatcctt
     7201 tagttccttt ttttcggtca aaagcttttc cagtcgaagc ttttccgctt gataggctcc
     7261 ctcaagcgcg ctcatcgttt ccgacggtat accctttaag ccttctggtg gtttcttttt
     7321 ttcgaatcct actttagaga gagctgtctc gaaagcaaag cgacccttgc ggctcgaagc
     7381 tgcaagaata gtcccttctt cgtctagagg aattatgact gctctatctc cgagcatctg
     7441 agaaagctct tggattctct cttttgcgac cttgccgatc tgaatcgata cataggaaaa
     7501 tttctcaaat tcttcgaagg gacgcgaaag gctggaaaag gcctttgcct catggataga
     7561 ttctgcaagc ccatctactt tggcctgttc ctcatctata tccagcttta gctgagagca
     7621 gcgctcgaag agcggctcga gcttcgaaag catatcctca tccggcaatc ggctgtcttc
     7681 gataatatcc ttcgggcttt cgataccgag gaactggcct ataaaatcaa gttttctttg
     7741 tgcctcatcg agccgttcac catcgagttg cgtctcatcg atctgttcgt tttccagatg
     7801 tgcctcgttg aactgaactt cgccgattgc aggtttgcct atcgttatct cgcccggaga
     7861 cgcctcacgg atagcattgt tgctgaccaa cgattctgcc ttgtccaccg ctctaaaact
     7921 tgctcttttt tctctcgcta gccgctcgag ctcatcggga tatatgacct gaaaacagcg
     7981 cttagttgca aggtatttta ggaccgcgtc tatatcggac ttgagaagca atagctcgac
     8041 tttgcgcata ctaacgctgc tcatgctgtc tctcctcctg gaatccacac atatttcatc
     8101 ttttcatccg aggtcgcgcc tagcttagta ctctcaagta ccgctaagag gagcgatgtc
     8161 tcataatcta aaagcttaaa ataacagtaa attggcgtat agctaaatgg gtagagatgc
     8221 agactccgcc tcaccataaa aaacaagtct tccagagcac tgatctccag agcaggaaca
     8281 tcgagacctg gcccagtctg gtttgacaat aatttcttga gtcgccaaga atcgaaactc
     8341 tcgactctct caagatcata ggaaaaagtc tctaacgcaa gcgaagtagt gtcctcttgc
     8401 ggcaaatcga cgagcgtatc tgcgatatcc tctttcgaca ttcggtaata tcgccgtaca
     8461 cgaatagccc acacaatatt ctgatagatt atttcccagc gtatcaaatc tagaattgct
     8521 cccactttcc gccttggcaa gccgagcgct gccttccaca attcaagata gtattgttta
     8581 tcgagctcgt tttccgccat cgctatgtta tctagccaat gactgtcgag ccatgaatag
     8641 ggggtatctg agaacatatc tgcgatgcga ggccatctag ctagggcttg ctctataacc
     8701 ttggaaaaat aagtcgtccc ttcgggcttt ggttcgccag aacgcatcct gaagagcatc
     8761 gattttatgg tgctaatctc atacttcgat atcaatgcac ggataaactc gttggaatcg
     8821 gaaaagggtt ctgttaggcg aacaaagtca tcgatggcgc gctttattat tctgcgttcc
     8881 gcctcgaaga caagccttgg ctcaggaatg ttcggaggct cctcatcaaa aagcagtttc
     8941 caaagagcta ggatacctcc tgagttttgc atgagctctc cggccttctc gcccagtctc
     9001 atattcgaaa aagctccgca gacgcgagca taaagatagg cgatatcgag cgtttcgggc
     9061 atatcagttt tccgtttgta ggagagcctc caatgtctcc aaaagcgctt tctggtttag
     9121 aggaatggaa ttaagactta agcgatattc atcgatctcc ttgtggctct ttaggttaag
     9181 agaatcgaca taggatgcaa aatctgcctc gagttccttg cgcatttggg tgattttctg
     9241 attttgggct atttccgact gagcttttgc ttcgattagc aatctggaag ctttttcttc
     9301 ggcttcctgg attatctgag cggcttgctg ctctatcttg agcaatttgt ctatgttatc
     9361 tgttgaatcc ataacaaggc ctcatggatt gagactatct tcgaattctc gaatcagagc
     9421 atataccttc tcgggcgttg tagccttcat gaggttctca agcaagagag gatcttgaat
     9481 aagaagcgca atctgccgca aggcttcgag atgcgcctcc gcttggctgg gaggagaaac
     9541 gagcatgaat acaagataca ccggattccc gtccaaagag tcgaagtcta tgcctctttt
     9601 ggatatacct atcactccgc gaagcttatc gatgccttcg aatgtcgcat ggggaaccgc
     9661 gactcccttg taaataccgg tagacatctt ctcttctctt tttcgaagcg ctctcaaagc
     9721 ctcttctctc ggaaataact gatcttttgg atagtccttt gcaagaatat cgaccaattc
     9781 ttcgagaagt tcgtctttgt attcgctttc cagctcgatc tttattgtag ctggcttgaa
     9841 tgcgttctgg agtatcatcg caacttccct tctcctgttt cccttatcga taggttcagg
     9901 cagattagct taaatgatag tgaaagatac cctggatggc aaggcagcag gattcaatgc
     9961 cacaaaattg tcgtgtaccc gaattgaatt gtcggcgcgt cgcgtatcta agacgcgcat
    10021 ctaagacgcg tatctaagac gcgtatctaa gaatatatcg aagcttcgat atggatgagg
    10081 cattgagaaa aagccgatga agagcagagg atccttgcct ttggctttga attgccatat
    10141 tgcagataag cctcaaacag agtaaaaatt aagatgctat agcactcacc caagggaaaa
    10201 tacatgctga ttattgagtc cataatactt ggaatcatcc agggcttggc tgaattcatt
    10261 cctatctcga gctccgcaca ccttatcatc gttccatggc tctttggatg gagcaaccct
    10321 gcgcttacaa gcctcacctt cgatgtagcg cttcatcttg gaacgctcct ggcggtcatc
    10381 gtgtttttcg cctcagattg ggcgcggctc atctcggctt ggttcaagag tgtagttcaa
    10441 ttcaaaatag gcgacgatcc tgatcggcgt atggcctggt acatcttgct agcatgtatt
    10501 ccaggcgggg tctcgggcct cttgctagaa tccaaaataa acgaagcttt tcattcagag
    10561 tctataccga ggagctcgat gctctttatg gccggatcga tcgctctgct tgcccttctt
    10621 ctctggattg ccgacaaata tgcacgccac cagaaaaatt ttggccagat aaaagctcgg
    10681 gacgctctct gcattggcct tgcacaggct tttgcggtca ttccaggtgt gtcgcgctcg
    10741 ggttcgacca taacggcagg acttgcactt ggactcgaga gagaagctgc agctcgtttt
    10801 tcctttcttc tgtctgcacc tatcatagct ggagcgggcc taaaaagcct ttatgacttt
    10861 tcagagcaaa taggtgcagg aaccattgct aaaagcgaat tatccatttt cccgattgga
    10921 ttcattgtgg cggctttgag tggctttttt tgtattaaat atctcttgtc ttacctcaag
    10981 aagcactcga cagaggtatt cgtatggtat cgattcgcgc ttgccgcttt ggtggtagca
    11041 gtctccttga ttcggggata ggctattttc ggataattga ttgatgcaga taattgattg
    11101 atgtagattt cctttttgaa tgtttggatc ggagacgact taactcgccg gcatttcttt
    11161 gagaaataaa gcgataggcg cttcctcttt ctattttgaa agtattttgg atcgagatta
    11221 ttggaggaat agcaatgaaa atcgtactta tcggcgcagg cagcgcacag ttcggattcg
    11281 gtactctggg cgacattttt tcgagcagct tgctcaaagg ttctcatatt gcgctcgtcg
    11341 atatcgatgg agagactctg agccacgtat ataatgccgc acaggccttt atcgatgccc
    11401 atggactcga ctttagccta tccgcgcaca cagaccgaag agaagctctt aaggctgcgg
    11461 attttgtcgt catctccatc gaagtaggcg accgcttcga gctctgggac caagattgga
    11521 tgatcccctt gcagtatggc atacctcagg tctatggcga gaatggcggc gcaggcgggc
    11581 tttttcatgc actccgaatc gtgccaccta ttctcgatat ttgtcaggat tgtacagagc
    11641 tctgtcccaa tgctaccatc ttcaatttct ccaatccgat gacttcgata acaacaacag
    11701 tcctgcgaaa attcccacag cttaagttca tcggactatg ccatgaaata gcctcgctcg
    11761 cgcgctatat cccgccaatg ctcggtgtcc ctcttgataa gctagaactc agagctgctg
    11821 gactcaacca tttcagcgtc ctagtatctg ctcgctatcg cgacacagga gatgatgcct
    11881 atcccgaaat acttgccaaa gcgccagact ttttcgaaag aatgattggt tattcagata
    11941 tactggaata catccaggag actggaaata tacctaaaac cgaaggcgaa acggatttgc
    12001 ccaatattgg tcgtactgtt tcgtcgaggc attgggccga cagaaggctc ttccgacaaa
    12061 tcttggagcg tttccatctt ctgcctatca cctccgacag ccatattgga gaatatatcc
    12121 cctgggctca cgatgtcgcg gacaataagg gaatactcga tttctattta ctgtatcgca
    12181 gctccttgag tatgctcgag ccaaagatag aactcaaggt taaagagcgc ttggtaccga
    12241 ttatcgaagg gatcctccaa gactcgggct acgaagaagc ggctgtcaat ataatgaaca
    12301 atggacttat cccaagcttg ccccaaaatg tgactgtgga agtacccgcg ctcgtgcata
    12361 gaaagggcat tgaaggtgtg gcatttccaa attatccaag agctttcgca gctctcttgc
    12421 gcaattacac tggcgtctac gatctcaccg cagaagcgat tctccataaa ag
//
LOCUS       DAOMZL010000050        12428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_4723_length_12428_cov_12.194617, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000050 DAOMZL010000000
VERSION     DAOMZL010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 12428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12428
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4723_length_12428_cov_12.194617"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2115
                     /locus_tag="PLQ30_05400"
     CDS             <1..2115
                     /locus_tag="PLQ30_05400"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPW01915.1"
                     /translation="REVVRGEYDIVLKAMDKVMLYTTVAPKVAHTAQSAMEVQIIEAP
                     TGEGPAIVPAPAQTTIAPSPSAGESGIETDTGLFLEVVYVSGKIRYEGPYARTPKLML
                     SSVVTPDQILQNTNLDYAELTRRKADGSWEYTTFSPRKVLNGSYDLPLRGMDSIRFVP
                     AKYLPEKIDFDKFSNAIAIVGIANFPGLYSFSEPKMISQVIGPEQLLSTTDIYYGEIE
                     RWKEGGRREYLTFNPIAVLQGHQDFQILQRDIIRFVAAGDTGENHDFSKYPDTVLFKG
                     SIRYPGRYAWYEGMKLSDIVHEQDLLIDTETSYAEIHRHTETKDTIINFAPDKVVTKK
                     TEIALSPRDNVIFYPKYYQKPVSIAGEVANPKIIPYYDQMELSSILRSVTLTRAYSDL
                     KAEIKRITGETTAVYLESYLKEQPTSKIILSPGDSITIRALLPDEHLPVVIVRGEVSQ
                     PQTLPFVEGMKLADALSAAGGYEATAYPKGLVLIRKSVATAQQTQVDRLIAQLEAATA
                     AGTALPTTTESTQTSASAVIANMQIDLAVQKAKLGNLKQLYKEGFGRISLDIPGSLEK
                     LSTSPGNIRLERDDLIYIPKIPTYVLVSGEVSSQNVVLYRDGMRVRDAITESGWLSNE
                     ADLAKAYIIRASGKLDSTEGKGFLFFKPNILNYKLEPGDTVYVPMKSAKVSVAWAYVR
                     DSFVLVSSILTSALTAKTLLGL"
     gene            2143..3342
                     /locus_tag="PLQ30_05405"
     CDS             2143..3342
                     /locus_tag="PLQ30_05405"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HPW01916.1"
                     /translation="MADQNSSVPESITLIDLAATIWKQKWLIIIITVIAAVFSVLYSL
                     SKPNIYTATSTLLPISGSTSALSQYAGLAAMAGVSLPGASSGDPTVKIQAILNSRELA
                     TKVINELDLIPKLIKEPEKLKTISPLSAALQAFQKNIFSVSVDTNTSVIKVSAKTKDP
                     ELSARIVNTAIDLLQQDLANRVLTASGKNIVVLEQQAAEQEKKVRELQNKMADYQKKN
                     KLIAPQTQSQQGLDLYRSLIQQKITLEIQISSLENALSADNPKISQAKAQLAAIQKQI
                     DDFEKTGGGVGPSMTETPKALFEYANLQAELELATKIYGGLLSSLENLRLQDATEKVF
                     VEVIDPAVPPELKSEPARATMCIVGTLAGGFLGLLLAFAQQALQKLIADPEVRAKFAK
                     KRETNSI"
     gene            3862..4479
                     /locus_tag="PLQ30_05410"
     CDS             3862..4479
                     /locus_tag="PLQ30_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HPW01917.1"
                     /translation="MFSPIILIGGFVEIVELCESCKREIVGIIDPNLFESYLNIPVLG
                     NDKDAEKIISQNKNCEFLIGLDDPNSRSRLFAYYSTFNVRFATVISPLARLSKYVSIG
                     NGSIIQHDVNVSAFSKIGDFAKLNYYSNITHNVELGDFVTVGPNAVLLGYVKIGQRSY
                     IGSNATILPRREIGQSVIIGAGAVVTKDISDEQIWVGNPATKLVR"
     gene            4569..5987
                     /locus_tag="PLQ30_05415"
     CDS             4569..5987
                     /locus_tag="PLQ30_05415"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPW01918.1"
                     /translation="MKSNLGSFSKNVAKFSIATWVSFAISLISAPISTRIYSPDSLGR
                     INLFYTYSSMLMSLAYLGLDQAYVRFYNELDDKKAKDSLFSFCLFISILTVLLLSLVA
                     FVFHDKVSTEIVGEKGFRFVFLLAISATTGILSRYLSLFYRMQIDGKWYTIQAVIDIL
                     FVKFSYLVSGYFNSSYQSAILVMVISRFVVICSFYLFQKKRFNFIIDNVNKEFSRDII
                     KFALPLVPITFLSWANSSVSQIVLKNMLGYSDLGIYSIAVSLASTVNIVQAGFNVYWA
                     PYVYNNYKSEKNIFNQVHQYMIFLLLLISLLIIFCQDPIFIILGEKYRNAKLFFPFLL
                     FSPICYTVAETTGLGINISKKTFWNLIIFSINIVVNIVGCFFLIPAFGVSGAAMASCI
                     SSMLYIVLKTIIGEKYYRSIESYRYIIYLLLILSVSALVNYFGYEKIELKYMAFIIIS
                     ILYAYLFRKEIRSMFRFLHKDT"
     gene            6039..6734
                     /locus_tag="PLQ30_05420"
     CDS             6039..6734
                     /locus_tag="PLQ30_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPW01919.1"
                     /translation="MKAVILAGGLGTRLKPFTNVIPKPLMPIGEKAILEIQIERLVKY
                     GFDEILLATNYKSDYIERFIGDGKRYGAKLILSKEDKPLGTAGPIKLLENQIKEPFLV
                     MNGDILSLVDFAKFYDFSVSQDSLFTVGIKKIIMPYAFGNIHFEGDIVTGIEEKPDII
                     TYALAGIYVMKPGIFSLIPKNQYFGMDNLIHNMLANNLTITKYEIKEYWIDIGRVDDY
                     EKIQEISETFTNK"
     gene            6743..7876
                     /locus_tag="PLQ30_05425"
     CDS             6743..7876
                     /locus_tag="PLQ30_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01920.1"
                     /translation="MKVLIIGDGNSIWIRDYIKNVLIANNATVFLYTGRISNEEYLQF
                     YKANNVVLFGYIDNSFIQKFPIIRGVLNILYSINPVYRNRYYDAIHVHFADKQSCWKA
                     HFYHKLAKKEIYSFWGSDLFRASDFQLKRLKSYLDKCHMITVATQKMHDRIISVFGNI
                     YDNKIRTLRYGIESFDIIDNIKKLEDKKTSMAKLGLPNDKIILNIGYNASVNQQHLAI
                     IEQLAKVDNKILDKLFIVIQMTYGNLNIDYHEEVREKIRSITNNFKIFENFMQTEDIA
                     RFRCCADIFIHGQTTDALSASFQEYIYAGAVVLNAKWLQYNELEERGIRYIEFGSFEE
                     IPMLVTKIVSNLEEYKLAFSNNRDILRAFSSVEAVSQEWYSIY"
     gene            7915..9006
                     /locus_tag="PLQ30_05430"
     CDS             7915..9006
                     /locus_tag="PLQ30_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPW01921.1"
                     /translation="MISISLIGCGRISSKHIESVLANKERLRLVACCDPVIDRAQRRV
                     QEYRTGSSGADAKVYADYREMLKKEKPDICAIATESGYHPRIAIDCLEAGSHVICEKP
                     MALSTHDADAMIAAARRNNKKLAVCFQNRFNAPVQRARAAVEAGRFGKMLHGMIQVRW
                     NRDENYYKQAPWRGTWDLDGGTLMNQCMHGIDLLQWMMGEDAVRVQAATRRFLRPIEA
                     EDFGAAIIEFASGAVGIIEGSAVVYPKNLGETLSLFGEKGTVVVGGLAVNKVKTWRFA
                     DAATVGDTEEKVLDPTAKDPPTVYGFGHTALYADFLDALEQNREPLVNGEAGKKALEL
                     ILAIYKSQKNSQPVTLPVDFSMSEMKGTF"
     gene            9006..10130
                     /gene="wecB"
                     /locus_tag="PLQ30_05435"
     CDS             9006..10130
                     /gene="wecB"
                     /locus_tag="PLQ30_05435"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPW01922.1"
                     /translation="MLSLLSVVGARPQFIKAAVVSRLIQSSQYCDKINECLVHTGQHY
                     DDNMSAVFFREMEIPEPDINLQIGSGPHGAMTGAMLAQLERVMMEQKPDVVLVYGDTN
                     STLAGALAASKLHIPVVHVEAGLRSFMMAMPEEQNRIIADRLSTVLFCPTEAAIDNLV
                     HEGIQFAQPALPSADHKAVFLSGDVMLDASLYYRQKQKAMSLSSALASLVERPFYLVT
                     LHRAENTDDLVRLASIVKALNEFDTLPAILPLHPRTRKILSQQGLEFAAHIHVIDPVG
                     YFDMLAIENRCEFIVTDSGGVQKEAYFFGKPCITLRDSTEWIELVQHGWNTLVGANTE
                     KILAALQSMPRYGDTVQLYGDGHAGEFIVQTLFNIFAKEG"
     gene            10132..11253
                     /locus_tag="PLQ30_05440"
     CDS             10132..11253
                     /locus_tag="PLQ30_05440"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPW01923.1"
                     /translation="MQVPFYTPVREYHARKAEFDHAVQQVFENGDFILGEDVAAFEKE
                     AASWLGAKYAVGVASGSDALVIGSDILGFRDGAEVLTPTYTFFASASCLARLGAKPIF
                     VDMDEETLNMDMRQAAEKVNSRIQGIIPVHLFLQPTPLQEVMDLARAYNIKVLEDAAE
                     GWGMESFVDGRWKKAGAVGDIGIYSFFPTKTLGAYGDAGLMVTDDEALYRKIKMYRVH
                     GTTTKYHHEYIGYNSRLDTLQAAILRVKLATTEQAIAARARHARHYTERLRAIPGLQV
                     QSVRSDAHPVYYVYNILVPQRDALADYLKENGIGTTIYYPKPLHLQPCFAYLGYKEGD
                     FPVAERICKDILALPMYPELTDDEVDYVCDAISSFYGRA"
     gene            11253..11858
                     /locus_tag="PLQ30_05445"
     CDS             11253..11858
                     /locus_tag="PLQ30_05445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPW01924.1"
                     /translation="MANSELIELPSADGGTFMRHESTYVDKGALVGSGTRIWHFSHIM
                     AGAKIGKNCSLGQNVNVASRAVLGNNVKVQNNVSIYDDVIIEDEVFCGPSIVFTNVIN
                     PRAFIERKHEYKKTIVGRGASIGANATVVCGVTLGKYCFVGAGSVVTKDVPDYALVYG
                     VPAIQNGWVCICGMKLDENLACPECGRKYRKTDKGIEEINE"
     gene            11851..>12428
                     /locus_tag="PLQ30_05450"
     CDS             11851..>12428
                     /locus_tag="PLQ30_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HPW01925.1"
                     /translation="MNNRELLNKIENNEEIVGIIGLGYVGLPLAVSFAKKGIKVLGFE
                     KSENKASLVNAAQNYIKDVDSEVLKACVREGKLSTTTDMSRIRECDALIICVPTPLDH
                     FKKPDMSFIESACIDIGRNMKRGVFISLESTTYPTTTEDVVKPIVEHESGFKEGEDFW
                     LCFSPERVDPGNKDYKTENTPKVVGGLGEEAQ"
BASE COUNT         3639 a         2484 c         2836 g         3469 t
ORIGIN      
        1 agggaagtgg tgagaggtga gtacgacata gttcttaagg ctatggacaa ggtgatgctg
       61 tatacgacag ttgctccgaa agttgcgcat actgcacaga gcgctatgga ggtacagatt
      121 attgaggctc ctactggaga aggccctgcc atagtgccag ccccagcaca aactactatt
      181 gctccttctc cttctgctgg cgaaagcgga atcgaaactg atacaggcct ttttctcgaa
      241 gttgtatatg tatctggaaa aataagatat gagggtccat atgctcgcac accaaagttg
      301 atgctctcct cggtggtgac gcctgatcaa attttgcaaa acaccaatct cgactatgca
      361 gaattgacgc gcagaaaagc cgatggaagc tgggagtata ctacattctc gccgcgcaaa
      421 gtgctcaacg gatcctacga tctaccgctg cgaggaatgg attccatacg ctttgtacca
      481 gcgaaatacc ttcctgagaa aatcgatttc gacaaattca gcaatgcaat tgcaattgta
      541 ggcatcgcga attttccagg tctctattca ttcagcgagc caaagatgat ttcgcaggtc
      601 atagggcctg aacaactact ctcgacaacc gatatctatt acggtgaaat tgagcgctgg
      661 aaggaaggcg gaaggagaga atacctcacc ttcaatccca ttgcagtgct ccaagggcac
      721 caggattttc agatacttca aagggacatc attcgctttg ttgctgccgg cgatacaggg
      781 gagaatcatg atttttcgaa atatcctgat acagtgctct tcaaaggctc gattcgctat
      841 cctggccgct atgcctggta tgaaggaatg aaactttcag acatagtcca tgagcaagat
      901 ttactcatag atacagagac aagctatgca gaaattcatc ggcacacaga aacaaaagat
      961 accatcatca attttgctcc cgacaaggta gttaccaaga agactgagat tgccctgagt
     1021 ccgcgcgaca atgtcatctt ctatcccaaa tattatcaga agccagtgag catcgctgga
     1081 gaagtggcca atcccaaaat cattccctat tacgaccaaa tggaactttc atccatcctg
     1141 cgaagtgtaa cacttactcg cgcttatagc gacctcaaag cagagatcaa gagaatcacg
     1201 ggcgaaacaa cggcggtgta tctggagagc tatctcaagg agcaacccac gagcaaaatc
     1261 atcctttcac caggggattc aatcaccatc cgagcgctcc tgccagatga gcacctacct
     1321 gttgttattg tgcggggtga ggttagtcaa ccacagacac ttccctttgt cgaaggtatg
     1381 aagcttgccg atgcgctcag tgctgcggga ggctatgagg ccactgcgta tcctaaaggc
     1441 ctcgttctca ttcgaaaatc cgtcgcgaca gcccagcaga ctcaagtgga caggctcatt
     1501 gctcagcttg aagctgcgac agctgctgga acagccttgc cgacgactac ggaaagcaca
     1561 cagacctcag catcagcagt gattgcgaac atgcagatcg atctagccgt acaaaaggcc
     1621 aaacttggca atctgaagca gctctataaa gaaggctttg gtcgcatctc gctcgacata
     1681 cctggttccc ttgagaaact ctctacatcg ccaggaaata tacggctcga acgcgatgat
     1741 cttatttaca tccccaagat tccgacctat gtgcttgtct cgggcgaagt atcgagccag
     1801 aatgtagtgc tctatagaga tggtatgagg gttcgcgatg caattactga atcgggctgg
     1861 ctctcgaatg aagcggatct cgcaaaagca tatatcatcc gtgcgtcagg taagctcgat
     1921 agtaccgaag gcaaaggctt cttgttcttt aagccgaata tcttgaatta taaactcgag
     1981 ccaggggata cggtctatgt gccgatgaaa tctgcgaagg tctcggtggc ctgggcctat
     2041 gtgcgcgata gctttgtcct ggtgagcagt attctcacga gcgcgctcac ggcgaagaca
     2101 ttgcttggtc tatagaattg cttagaattc aaggagatat gtgtggctga tcaaaatagt
     2161 tctgtaccgg aatccatcac tttgatcgat ctggctgcca ccatctggaa gcagaaatgg
     2221 cttattatta tcataacggt aatagcagca gtgttttcgg tgctctattc actcagtaag
     2281 cctaatatct atactgcaac gagtacctta ctgccgattt caggctcaac ctcagcgctt
     2341 tcacaatacg cggggcttgc agcgatggcg ggcgtgagcc ttccgggggc tagctctgga
     2401 gatcccacgg tcaagatcca agcaattctc aatagccgcg aacttgccac taaagtgatc
     2461 aatgagctcg atttgattcc aaaacttatc aaagaaccag agaaactaaa gacaataagc
     2521 ccgctctccg cagcgctgca ggcatttcag aagaacattt tttctgtctc cgtcgatacc
     2581 aatacttcgg tgataaaagt gtcagccaag acaaaagatc ctgagctctc cgctcgcatc
     2641 gtcaatactg cgattgattt gttgcagcaa gacctcgcca atcgtgtgct caccgcgagc
     2701 ggcaagaaca ttgtggtatt ggaacaacag gctgccgagc aagaaaaaaa agtacgcgaa
     2761 ctccagaata aaatggcaga ttaccaaaag aagaacaaac tcatagcacc gcagacgcaa
     2821 agtcagcaag ggctcgatct ctatcgctct cttattcagc agaaaatcac cctcgaaatt
     2881 caaatttcga gccttgaaaa cgctctatca gcagataatc caaagatttc ccaggccaag
     2941 gctcagctcg cagccataca aaaacaaatc gatgattttg aaaaaacagg tggaggtgtc
     3001 ggtccatcca tgacagagac acctaaagct ctctttgagt atgcaaatct acaagctgaa
     3061 ctagagcttg cgacaaagat ttatggaggg cttttgagca gcctcgaaaa tttacgcctg
     3121 caagacgcca cagaaaaggt ctttgtcgaa gtgatcgatc ccgcggtccc tcctgaacta
     3181 aagagcgaac ctgcccgtgc aacaatgtgc attgttggta cccttgccgg aggatttttg
     3241 gggctccttt tggcctttgc gcagcaggcg ctccaaaaac tcatcgccga tccagaagtc
     3301 cgcgccaaat ttgcaaaaaa gcgagaaact aattccattt gactggattg tcaggattgg
     3361 ataggatgat cgggataatg tgtcgactgg atggctggat ggaaatagat aaacagggtt
     3421 ttcctttgaa aggatttcct atctggataa tttatttata tcctatcgct gtccctgtct
     3481 tattgcattc atttcataaa agacccccct tgtattttgg tgagactttg tctatacata
     3541 gaatgttcat tttgtgaaca ttcattttga agcacagcgg atgcctcttt aagtcattat
     3601 ggatgcttca aagacatact cttgccttga tcatcagcgt gttgcggccg cgatggaggg
     3661 ggagggtaac aacagtctcc ttattacagg taccgacggg tgttatcggt agcgctcttg
     3721 agatttggtc tttgcagaaa tatttgattg aatgtgtata agatatttaa tctttataaa
     3781 agattaggat tcccattaaa aatatacgat agatttaaag agactataaa catgatgtgg
     3841 ctatattaag gagttttgat tatgttttct cctataatat tgattggtgg ttttgttgaa
     3901 atagtagaac tatgcgaatc ctgcaaacgt gagattgtcg gaataataga tccaaatcta
     3961 tttgagtcat atctcaacat tcctgtctta ggtaatgata aggatgctga aaagataata
     4021 agtcaaaata agaattgtga attcttaata ggtctagatg acccaaattc cagatcccgt
     4081 ttatttgctt attattcaac ctttaatgtt agatttgcaa ccgtaatttc gcccttagct
     4141 agattatcaa agtatgtttc cataggaaat ggctcaataa ttcaacatga tgtaaatgta
     4201 tcggcatttt caaaaatagg ggattttgct aaattgaact attactcgaa tataacccac
     4261 aatgttgaat taggtgactt tgttactgta gggcctaatg cggttttgct tggctatgtc
     4321 aaaataggac aaagatcata tataggttct aatgctacca tattgccacg aagggaaatc
     4381 gggcagtccg taataattgg cgcaggtgca gtcgttacaa aagatatctc cgatgagcag
     4441 atctgggtag gtaatcccgc cacgaagcta gtaagataag cagtagggag ccaatatcac
     4501 tattaactca aagaggattc aagaattatg ttcattatta tcattgctta tgaaagtaaa
     4561 taccagtgat gaaatcaaat ctcggatcct tttctaaaaa tgttgcaaaa ttttcaatag
     4621 cgacatgggt atcattcgca ataagtttaa tatctgctcc tatttcgacg agaatctatt
     4681 ctcctgattc gcttggcagg attaatctat tctataccta ttcatcgatg ctaatgtctc
     4741 ttgcttattt agggttagac caggcatatg taagattcta caatgaatta gatgataaga
     4801 aagcaaagga ttcattattt tctttttgtc tttttatttc gattttgaca gttttattat
     4861 taagccttgt tgcttttgtc tttcatgata aggtctctac tgaaattgta ggagaaaaag
     4921 gatttcgatt tgtcttttta ttagcaatca gtgcgacaac aggcatatta agcagatatt
     4981 tgagcctgtt ttataggatg cagatagatg ggaaatggta tactatacaa gcagttatcg
     5041 atatcttatt tgtaaaattt tcatatttag tatctggata ctttaattca tcttatcaat
     5101 ctgcgattct ggttatggta atttctcggt ttgtcgtcat ttgtagtttt tatttgtttc
     5161 aaaagaaaag gttcaatttc ataattgata atgttaacaa ggagttttct agagatatta
     5221 taaaatttgc actaccattg gtgcccatta cctttctatc gtgggctaat agttcagttt
     5281 cgcagatagt gctcaaaaat atgctcggat attctgattt agggatctac tctattgctg
     5341 taagcttagc gagtacagta aatattgttc aagcgggatt caatgtgtat tgggcaccgt
     5401 atgtctataa caattataag agcgaaaaaa atatttttaa tcaagtacat caatatatga
     5461 tatttctatt gctcttgatt tctttgctca ttatcttttg ccaagatcct atatttatta
     5521 ttcttggaga aaaataccgt aatgctaagc tctttttccc ctttcttctc ttttcaccta
     5581 tttgctacac tgtcgctgaa actaccggac ttggtataaa tatatcaaag aagacttttt
     5641 ggaatctgat aattttttcg attaatattg ttgtaaatat agtaggatgc ttctttctaa
     5701 ttccagcatt tggggtttca ggtgcggcta tggcttcctg tattagctcg atgttataca
     5761 ttgtgctaaa gacaataata ggagaaaaat attatagatc aatagaaagc tatagatata
     5821 ttatttatct attacttatt ttatctgtat ctgctctagt taattatttt ggttatgaga
     5881 aaatagaact taaatatatg gcatttataa taatttctat tctatatgct tatctattta
     5941 ggaaagaaat aagatctatg tttagatttc tacataagga tacataaaag tatgtataaa
     6001 ttgaagtatt tattattgaa aatcattgtg gagatgatat gaaagctgta attctagcag
     6061 gtggtcttgg gaccagattg aagcctttta ccaatgtaat tccaaaacca ttgatgccaa
     6121 ttggcgaaaa agcaatattg gaaattcaaa ttgaacgatt ggtcaaatat ggcttcgacg
     6181 aaatacttct tgcaactaat tataaatccg attatatcga aagattcatc ggagatggaa
     6241 aaagatatgg cgctaaattg attttgagca aggaggataa gccattaggt actgctggcc
     6301 ccattaaact attagaaaat cagattaaag agccttttct tgttatgaat ggcgatattc
     6361 ttagcctagt cgattttgcc aagttctatg atttttcagt ctctcaagat tctttgttta
     6421 ctgttggaat aaaaaagata attatgccat atgcttttgg caatattcat tttgaaggtg
     6481 atatagtcac agggattgaa gaaaaaccag atattattac ttatgcacta gcaggtatat
     6541 atgtaatgaa accaggcatt ttttcgctta ttccgaaaaa tcaatatttt ggaatggata
     6601 acttaatcca taatatgctc gcaaataatt tgactattac aaaatatgaa ataaaagaat
     6661 attggataga cataggaaga gttgatgatt acgaaaaaat tcaagagatt tcggaaacat
     6721 ttacaaataa atgaggttta gattgaaggt acttatcatt ggagatggga attctatatg
     6781 gattagagat tatatcaaga atgttttaat agcaaataat gcaacagttt tcttatatac
     6841 tggaaggatc agtaatgagg agtatttaca gttctataag gcgaataatg tagtattatt
     6901 tggttatata gataattctt tcattcagaa atttccaata atccgcggag ttctgaatat
     6961 attatatagt ataaatccag tctatagaaa tagatactat gatgccatac atgtccattt
     7021 tgctgataag caatcatgtt ggaaggcgca tttttatcat aagctagcaa aaaaagagat
     7081 ttattctttc tggggaagtg atttatttag agctagtgat tttcaattaa agagattgaa
     7141 atcttatcta gataaatgtc atatgattac tgttgctact caaaaaatgc atgatcgcat
     7201 aatatctgta ttcggaaaca tttatgacaa taagataagg acactccgtt atggaattga
     7261 gtccttcgat attatagaca atattaagaa gctagaagat aaaaaaacaa gcatggctaa
     7321 gctaggacta ccaaacgaca agataattct aaatattggc tataatgctt cggtaaatca
     7381 acaacattta gcaattatag aacagcttgc taaagttgat aataagattc ttgataagct
     7441 ttttattgtt atacaaatga catatggaaa tctaaatatt gattatcatg aagaggtgag
     7501 agaaaaaatt agaagcataa ctaataattt taagatattt gagaatttta tgcagacaga
     7561 agacatcgct aggtttcgat gttgtgcgga tatatttatt catggacaaa ccacagatgc
     7621 cctatcggct tcatttcagg aatatatcta tgctggtgcg gtcgtactaa atgcgaaatg
     7681 gttacaatat aatgaattag aagaaagagg cattcgatat attgaatttg gatcttttga
     7741 ggaaattcct atgctcgtga caaaaatcgt aagtaatttg gaagaatata agttggcctt
     7801 ttctaataac agagatattc ttagagcatt ttcatctgtg gaagcagttt cgcaagaatg
     7861 gtatagcatt tattaggagt cattaataat tttaagatta aggaataatt atagatgata
     7921 agcatatctc ttatcggttg tggtcgcatt agttcaaaac atatcgaatc tgtgctagcc
     7981 aacaaagaac gattgcggct cgtcgcgtgt tgcgacccgg tcatcgatcg tgcgcaaagg
     8041 cgcgtgcagg aatatcgcac aggttctagt ggagcggacg caaaagttta tgctgactat
     8101 cgcgagatgc tcaaaaaaga aaagcccgac atatgcgcca ttgcgacaga gtcgggctac
     8161 catccacgca tcgcgatcga ttgtcttgag gccggtagcc atgtcatctg cgaaaagccc
     8221 atggcgctct ctacgcacga tgcagatgcg atgatagcag cagcgaggcg taacaacaaa
     8281 aagctcgcgg tctgctttca aaataggttc aatgcgccgg ttcagcgtgc tcgtgcagct
     8341 gtcgaagcag gtcgctttgg caaaatgctg catggcatga ttcaggtgcg ctggaataga
     8401 gatgaaaact actacaagca agctccatgg cgcggtacct gggatctcga tggcggcacg
     8461 ctcatgaacc agtgcatgca cggtatcgat cttttgcaat ggatgatggg tgaggacgcg
     8521 gtgcgagtgc aagcagccac gaggcgcttt ttacgcccta ttgaggcaga agattttggc
     8581 gcggcgatta tagaatttgc aagcggcgcg gtgggcatta tagaaggaag cgccgttgta
     8641 tatcctaaaa acttaggcga gacgctctca ctctttggag agaagggaac agtggttgtc
     8701 ggcgggctcg ccgtcaacaa agtcaaaact tggcgttttg cagatgcggc aacagtgggc
     8761 gatactgaag agaaagtcct cgacccgacc gcaaaggatc cgccgacagt ctatggtttt
     8821 ggccatacgg cgctctatgc tgatttcctc gatgctctag agcagaaccg cgagccactc
     8881 gtgaacggcg aagcaggaaa gaaagcgctc gagctcatac ttgcgatcta taaaagccaa
     8941 aagaatagcc agcctgtgac gctgcctgta gatttctcta tgtctgagat gaaagggaca
     9001 ttctagtgct ctctcttttg agcgtggtag gagcacggcc gcaatttatc aaggctgcgg
     9061 tggtttctcg gcttatccag agtagccaat attgcgataa gataaacgaa tgccttgtgc
     9121 atactggcca gcactacgac gacaatatgt cggcagtgtt ctttcgagag atggagatac
     9181 ccgagccaga cataaacctt caaataggct ctggacctca tggagcgatg accggtgcga
     9241 tgcttgcaca gcttgagcgc gtcatgatgg agcagaagcc cgatgtcgtt cttgtctatg
     9301 gcgatacgaa ttcaacgcta gcaggggctt tggcggcgtc aaaattgcac attcctgtgg
     9361 tgcatgtcga ggcaggcttg cgttctttca tgatggcaat gccagaggaa caaaatcgca
     9421 ttatcgcaga taggctctct acggtactgt tttgcccaac agaggctgcg atagacaatt
     9481 tggtacatga aggaatccag ttcgcgcaac cagctttacc tagtgcagat cataaggcgg
     9541 tgttcctttc cggcgatgtg atgctcgatg catcgctcta ttaccgtcag aagcaaaaag
     9601 ccatgtcttt aagctcggcg cttgcatcgc ttgtagaaag gcctttttac cttgtgacct
     9661 tgcatcgcgc agagaacact gatgatcttg tgcgacttgc atcgatagtg aaggccctta
     9721 atgagttcga tacattacct gcaattttgc cgcttcaccc tcggacgaga aaaattcttt
     9781 ctcaacaagg acttgagttc gctgcccata tccatgtgat cgatcctgtt ggctatttcg
     9841 atatgcttgc catcgagaat cgctgtgagt ttattgtcac cgattcaggc ggtgtacaga
     9901 aagaagccta tttctttggt aagccatgta ttacgcttcg cgattcgaca gaatggatag
     9961 agcttgtgca gcatggctgg aatacgctcg tcggtgcaaa taccgaaaag attcttgccg
    10021 cactgcagtc gatgccacgc tatggcgaca cagtgcaact ctatggcgat ggtcacgcag
    10081 gcgaattcat cgtacaaaca ttgttcaata tctttgcaaa agaaggctga tatgcaagtt
    10141 cccttttata cgccggtgcg cgaatatcat gcacgcaaag cggagttcga ccacgccgta
    10201 caacaagtct ttgaaaatgg cgatttcatc ctaggcgaag atgttgcggc ctttgaaaaa
    10261 gaagcagcgt cgtggcttgg ggcaaagtat gcggtcggtg tggcctctgg ttctgatgcg
    10321 ctcgtaatag ggagcgatat tctcggattc cgcgatggcg ctgaagtact gacgccgacc
    10381 tatacattct ttgcttcagc atcctgcctc gcgcgtcttg gcgcaaagcc aatctttgta
    10441 gacatggatg aagaaacgct caatatggac atgcgccagg ccgctgaaaa ggtgaattcg
    10501 cgaatacaag gcattattcc tgtgcatctt tttttgcagc caacgccgct ccaagaggtg
    10561 atggaccttg cacgtgccta taacatcaaa gtgctcgaag atgccgcaga aggctggggc
    10621 atggaatcgt ttgtcgatgg caggtggaag aaggcagggg cagtaggcga tatcggcatt
    10681 tattcattct ttcccacgaa gaccttggga gcctatggcg atgcagggct gatggtcacc
    10741 gatgatgaag cattatatcg caaaataaag atgtatcgcg tgcatggcac cacaacgaag
    10801 tatcatcatg aatacattgg ctataactct cggctcgata cattgcaagc agcaatcttg
    10861 cgagtcaagc ttgccaccac agaacaggct attgctgcaa gggcgcgcca tgcacggcat
    10921 tataccgagc ggcttcgtgc aattccgggc ctgcaagtcc aaagcgtgcg cagcgatgct
    10981 catccagtct attatgtcta caacatcctc gtgccgcagc gcgatgcgct tgcagattac
    11041 ctcaaagaaa atggcatagg aacgacgatc tattatccaa agccgctcca tttgcagccc
    11101 tgttttgcct accttgggta taaagaaggc gattttcctg ttgcggagcg catctgcaaa
    11161 gatattctgg cgctccccat gtacccggag ctcaccgatg atgaagtgga ctatgtgtgt
    11221 gatgcgattt cgagctttta tgggagagca taatggccaa ttctgagctg attgagctgc
    11281 cttcagccga tggaggcacc tttatgcgcc atgagtccac ttatgtagac aaaggtgctc
    11341 tggttggctc tggaacccgc atttggcatt tttcgcatat catggcaggc gcaaagatag
    11401 gaaagaattg ctcgcttggg cagaatgtca atgtggcaag tagggccgtg cttggcaata
    11461 atgtcaaggt gcaaaacaat gtttcgatct atgacgatgt aataattgaa gatgaagtat
    11521 tctgcgggcc atccatagtc tttaccaatg tcatcaatcc tcgtgccttt atcgagcgaa
    11581 aacatgagta taaaaagaca attgtgggtc gcggtgcttc cataggcgcc aatgcgaccg
    11641 ttgtctgcgg agtaaccttg gggaaatatt gttttgtagg cgcgggaagt gtcgttacaa
    11701 aagatgttcc tgattacgcc cttgtatatg gcgttccagc tattcagaat ggttgggtat
    11761 gtatctgtgg catgaaattg gatgagaatc ttgcttgtcc agaatgtggc agaaaatata
    11821 ggaaaactga caagggaatc gaggaaataa atgaataatc gagagttgct gaacaagatt
    11881 gaaaataatg aagaaattgt tggcataata gggcttggct atgttggctt gccactcgcc
    11941 gtgagctttg caaaaaaggg aatcaaggtc cttgggttcg aaaaaagcga gaataaagct
    12001 tctctagtca atgccgctca aaactatatc aaggatgtgg atagcgaagt gctgaaagca
    12061 tgtgtccgag aaggaaagct ttcgacaacc accgatatgt cgcgcattcg cgagtgtgat
    12121 gcgctcatca tttgcgtgcc gacaccgctc gatcatttca agaagcccga catgagtttt
    12181 atcgagtctg catgcatcga tattggccga aatatgaaaa ggggtgtctt cattagcctc
    12241 gaaagcacga cctatcccac gacaacagaa gatgtcgtaa agccgattgt ggagcacgag
    12301 tctggcttca aagaaggcga ggatttctgg ctctgcttta gcccagagcg agtagatcct
    12361 ggcaataagg actacaaaac agagaatact ccaaaggttg tcggaggcct tggagaagaa
    12421 gcgcagaa
//
LOCUS       DAOMZL010000051        11608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_5198_length_11608_cov_11.347183, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000051 DAOMZL010000000
VERSION     DAOMZL010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 11608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11608
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5198_length_11608_cov_11.347183"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1477)
                     /locus_tag="PLQ30_05455"
     CDS             complement(2..1477)
                     /locus_tag="PLQ30_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPW01926.1"
                     /translation="MILANVAAALPGERDFRLVDIFIKGGKIASIKEASIKKKIENPP
                     KIGDPGTNEAADPYDYEDILDAHGLLAFPGAIDPHVHFDEPGFTHREDFLHGSAESAQ
                     GGVTTIIDMPCTSIPPVISDDALEEKLSIVQKKALIDFGFYGGVNGNMAPDSIPQVVA
                     SLAPRVLGFKSYFISGMESFPAVNEAQFVAAAQACGKEGRPLLLHAEDPYVISEATKE
                     RQALRGTQKPQWIDYYASRPMDSEIAAVMNALKLAGQYAKYVHIVHVGTAKAAIAAVG
                     RGATCETCAHYLAFDETDFSRFGAALKTAPPVKSPEQKAILWHLLANGKINFVTSDHA
                     GAPDYEKFTDDPLLAYGGIPGTGTLFPYILSEGLFAKRLSLDRFLEVTSGGAARRYGL
                     WQGKGSLAPGKDADIVLVDPYHTRRIDPSAMMSKNAITPFAGMVLSGRIEGTWVRGSC
                     VYASVRLAAALKAPKDFVFSNEEGMILAKPGYGTFLTWGYR"
     gene            complement(1508..3916)
                     /locus_tag="PLQ30_05460"
     CDS             complement(1508..3916)
                     /locus_tag="PLQ30_05460"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPW01927.1"
                     /translation="MNNKDILELDESLTRKANSDMSQVWAGKVELESGSSGLIPESMP
                     NVLGQTQYIDDITRPAGCLQAVVVLSQSAHGRIRKIHTEEALELDTSVRVILASDIPG
                     TNQIGFNKPDEPLLPESEWDYWGQPLAIVVANSRILARRAASLVRIEGENLPEVIDPR
                     EAAAKGDFIFPPRTIACGDVREAFSRCAFIVEGRVDSGGQEHVYLETQGAIAQVIDGR
                     RVLVISSTQGPTGVQKAVAQVLGLPMSMVEVEARRLGGAFGGKEDQGANWACMAAVAS
                     WVTGKPVEIYLNRRDDMRATGKRHPYSTDFRLGADASGKLIAFEADYYQNSGSTCDLS
                     AAILARTVLHATGAYQIPNVRVTGYMCHTNLPSFTAFRGFGGPQAFFVIESAIDALAQ
                     KMGIDAVEIKRNNLFKEGDVTYYGMTMERVRASDSVERLLCKVDYPALKAKIAEFNAS
                     HRLEKKGIGLIPVAFGISFTKLQMNQAGALVHIYTDGSVVVSTGAIEMGQQVARKIAI
                     IAAKTLGVSIDRVTVQRTTTLTVANTVPTAASTGSDMNGMAARIACLEIRGRLLKKAA
                     ELLMVDLDKVDIKDGAVLLNGTPSHISWEQLVEAMSASRQDLSAHGFYATPTLEYDMK
                     TERGKPFAYHVYGAALVEASIDVLRGTCTIDRVTIVHDIGESIDQSVDIGQIEGALAQ
                     GLGWALLEDLRFEPNGKPISDTLSTYKVPDIGFMPPGIDIEFLPGIDNPFAPYNSKAV
                     GEPPLQYGIAGYFAVLEAIRAATGKALEYYDLPLIPEKISKLLVLEQDNNNRATANHI
                     RKKA"
     gene            complement(3913..5439)
                     /locus_tag="PLQ30_05465"
     CDS             complement(3913..5439)
                     /locus_tag="PLQ30_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPW01928.1"
                     /translation="MACLRIKVNNTWQDIRSDEGTPAIDIIRGELGLMATKEGCREGD
                     CGACAVLIGEYAAGQLRYLAVPSCLLALGELKGKHLITLEGLIEGTSGGLTPVMRAFL
                     DENASQCGFCTPGFIIALTSWLAEPRLLDLAGALRAIDGNLCRCTGYAAIRRAVRRLI
                     EQFKDLPLEPEARLNALVSAQVLPPSVREFADEASTQVLLNSNNDKENPELTPKQRMT
                     SPGFVILGGGTDYYVKNPEPNDGFSPFLIRMRPEFKEINYLDENGQRWLEIGAAVTVR
                     EFFASELIRQEVPGIEQFETMFASTLIRNLATIGGNIVNASPVADITAMLLALNASIV
                     IASQIQEGFDLSLTRTCKLEEFFLGYKTIDLKPTEVIKAIRIPSCNDPTARKFSFEKA
                     SKRKNLDIAAVNTAISFRIEEQRFKDVHISLGGVAPTPTVNKAAMELLEGSSCPVSDG
                     KIMAELAKRVASSAEASVRPISDVRGSEEYRRRMVYRLMLAHFVRIFEDSGVAEELFP
                     "
     gene            complement(5603..7333)
                     /gene="ade"
                     /locus_tag="PLQ30_05470"
     CDS             complement(5603..7333)
                     /gene="ade"
                     /locus_tag="PLQ30_05470"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HPW01929.1"
                     /translation="MKKDIIEAAMGKAQCDVNYTNCILVDVLSGRLLKDSVISIKNGY
                     VAGINDGLSAKETIDLKGMYLAPGLIDAHVHIESSLLTPAEYAKVVIPHGTTTVVADP
                     HEIANVMGYDGMRYMLNVSENIPLDVYFMVPSCVPATDFDTAGAALYASDMHQFLQEP
                     RVLGLGEVMNYPGVLAQDPQLMDKIALFKSNGRPIDGHSPGLSGARLSAYIAAGIGSD
                     HECTTAEEALEKVGKGMFIMLREGSTAKDLRNLLSAVRGENAGRFLLCSDDRHCNDLR
                     DEGHIDYSLRLMLDAGIDPIDAIRIASSNAAGWFGIPAQGAIAPGYKADFILFPSFEF
                     FEIKAVIKNGRIVARDGSLIEDFSAEPIAIRDSVNIKWLTAEDFVIPDKQSPVRVIRV
                     QPNSLLTIHGLERLPSENGALVSDLERDILKIFVIERHTGSGNIGKGFIQGLGLKRGA
                     IGSTISHDSHHMIIAGVDDLSIFKAARHLNKIKGGLVYAVGDQILLDLPLPVAGLMSD
                     KPADFVIEKLSDFDRLFLKEGLTATSPLMTLSFMALPVIPSLKITDKGLVDVEKFEKV
                     SLYVNETVKD"
     gene            complement(7330..8280)
                     /locus_tag="PLQ30_05475"
     CDS             complement(7330..8280)
                     /locus_tag="PLQ30_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW01930.1"
                     /translation="MDFITSILTITIRAGTSLTIATIGEILTERTGILNLGVEGIMLM
                     GALTAFATVFYSHSIMLGILAAIAIGGILALLHAFLTTTMRANQVVSGLSITLFGTGF
                     SSFLGQRLGPESNNFRLVGLRAERILPFAPNWAKDIPILSAFLNQDIMTYFVYLFIPL
                     AWFFLYKTRPGLWLRSVGEDPYTADAMGIDVTKTRYFYTIIGGMLIALGGAHLSLSYT
                     PGWSENITGGRGWIVIALVIFSMWNPARAVWGSLLFGGINAVQFRLQASGTSVPANFL
                     NMLPYIGTILVLVAITWWEALSKRVGAPAALGSSYMREEK"
     gene            complement(8290..9378)
                     /locus_tag="PLQ30_05480"
     CDS             complement(8290..9378)
                     /locus_tag="PLQ30_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW01931.1"
                     /translation="MRLVFEKRKTTSISALILVPMVSFAVSLFLTALLLMIFEADPLK
                     TYTAMAIGAFGSLYGFAETLVKAIPLMMTGLGVAIAFRLKFWNIGAEGQLALGGVAAA
                     GVALFMEQFLPGPLLLVGALLGGLLAGALWAGIPALLKTSLNVDETLVTLMMNYIAIL
                     FSEYLYYGPWRDPKGYGFPGSRMFASNAWLPRIVGRAHIGVLIALFLALVLWFILKRT
                     RWGFELQIIGASQQAARYQGIAVERNIVLAITISGALCGLAGAFEVTGISHRLQQGLS
                     IGYGYTAIIVAWMSQLNPLAVPFVAVALAALSVGGDQVQMTMGLPAAMGTVMQGLILF
                     PMLAGSLFTEYRLMAVKDNINSACRDAT"
     gene            complement(9375..10904)
                     /locus_tag="PLQ30_05485"
     CDS             complement(9375..10904)
                     /locus_tag="PLQ30_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW01932.1"
                     /translation="MIDSNIPRIEMRRISKSFPGVVANDCIDLKLHKGEVLALLGENG
                     AGKSTLMNILCGLYRQDQGEIYIDGQKVEIHSPRDAQKYGIGMVHQSFKLVNSMNVLE
                     NIILGLRDEPFIPDMKRVRKQLMELASQYHLNIDPDAYIWQLSVGEQQRVEILKLLYR
                     NAEVLILDEPTAVLTPQEAHELNRVIKTMKQEGKSAIFITHKMEEVIEFSDRVMVLQK
                     GRVVAETPTSETSPKDLARMMVGREVLFQIERKPYKPGKTILELSDIEALGDRGLPAL
                     KNISFSLRAGEILGIAGVDGNGQRELAEVITGLRKSTKGTLKIADRDMTNKSPLEIIC
                     HGVAHIPQDRASVGAVGDLSVASNLAMKQYRSRPLAQGIILLPKRIIEFARKLIDKFR
                     IATPSPGTQIKFLSGGNVQKTILAREIGAARSLMVAVYPSRGLDVGATESVRRQLINM
                     RDEGLGILLVSEDLDELLQVSDRIAVMFEGRFMDIFDAEKADIENIGMLMAGMNAEAI
                     R"
     gene            complement(10968..>11608)
                     /locus_tag="PLQ30_05490"
     CDS             complement(10968..>11608)
                     /locus_tag="PLQ30_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPW01933.1"
                     /translation="GRMTKTNKIGYVAAFGIPEVVRGLNGFALGVRSVNPNAKIQVVW
                     TNTWYDPVKEREAAIALLDAGCDIIAQHQDTTEPQKAAQERGKLSIGYDSDMAQFVGD
                     TVLASAIWNWGTYYIRTIQEVLDGTWKTHSYWGGLKDDIVSLSDLSPLVPANVRKEVE
                     NAKKKILGGWSIFTGPIKDQNGKIVYKAGEIIPDDKQLSMDWFVEGVVGKVQ"
BASE COUNT         2926 a         2939 c         2599 g         3144 t
ORIGIN      
        1 ctcatcgata cccccaagta agaaatgtgc cataaccagg tttggcaaga atcatgcctt
       61 cttcattaga aaatacaaaa tctttagggg cctttagtgc tgccgcaagt ctaaccgaag
      121 catatacgca agaacctcga acccatgtgc cttcgattcg tccggagagc accatcccgg
      181 caaaaggagt tattgcattc ttactcatca ttgccgaagg atcgatccgc cttgtatgat
      241 atggatcgac aagtacaata tcggcgtctt tgccgggagc aagcgatcct ttcccttgcc
      301 atagtccata ccttcttgct gcgcctccgc ttgttacttc caggaagcga tcgagtgaga
      361 gtctcttagc aaacaagccc tcagatagaa tatatggaaa taatgtgccg gtgccaggaa
      421 tcccgccata cgccaaaagc gggtcatctg tgaatttttc gtaatctggc gctcctgcat
      481 gatcagatgt gacaaagttg atttttccat tggcgagaag gtgccataga attgcttttt
      541 gttccgggct tttcaccggt ggagccgtct tgagagcagc tccaaagcga gaaaagtccg
      601 tctcatcgaa tgccagataa tgagcacagg tttcacatgt ggcaccgcgc cccacagccg
      661 caattgctgc tttagcagtg ccgacatgta caatatgaac atatttcgca tattgtcccg
      721 ctaattttag ggcattcatc acagcggcta tttcggaatc catgggacga gaagcatagt
      781 agtctatcca ttggggtttt tgtgtaccgc gtaacgcttg cctttctttg gtagcttccg
      841 agattacata aggatcctct gcatgcaaaa gaagaggccg cccttctttt ccgcaagcct
      901 gcgcggcggc gacgaattga gcttcgttta cggcagggaa tgattccatg cctgatatga
      961 aatatgattt aaaaccaagt acccgaggag ccagcgatgc tacgacctga ggaatagaat
     1021 caggggccat gttcccattg acaccaccat agaagccaaa atcgatcagc gctttttttt
     1081 gtacgattga tagcttttct tcgagtgcat catcagagat gacgggtgga atggaagtgc
     1141 agggcatatc tattatggta gtcacacctc cttgcgcgga ttccgcagag ccatgcagaa
     1201 agtcttcgcg atgtgtaaag ccgggctcat caaaatgaac atggggatct attgcacctg
     1261 gaaatgcgag aagcccatga gcatctagga tgtcttcata atcataagga tcagctgctt
     1321 catttgtccc aggatctccg atttttggcg gattttctat ttttttcttg atgcttgctt
     1381 ccttgataga agctattttg ccgcctttta tgaaaatatc gacaagtcga aaatcgcgtt
     1441 caccaggcaa tgccgcggcg acatttgcga gtatcatttt gcttctcctt gtcaaaaaca
     1501 ttccgcatta ggcttttttc ctgatatggt ttgcggttgc cctattatta ttgtcttgct
     1561 ccaatacgag caacttcgat attttttctg ggataagagg cagatcgtaa tattcgagcg
     1621 cttttccagt tgcggctcta atggcttcga gaaccgcaaa atagcctgct ataccatatt
     1681 gcagaggggg ctcgccgact gcctttgagt tatatggtgc aaagggatta tcgatgcctg
     1741 gtaggaattc gatatcgata ccaggaggca taaaaccgat atccggcact ttataggtag
     1801 ataatgtgtc cgaaattggc ttgccattgg gttcgaaacg caaatcctcg agcagagccc
     1861 agcccagccc ttgtgcaagg gctccttcga tctgacctat atcgacgctt tgatcgatac
     1921 tctctccaat atcgtgcaca attgtaaccc tgtctattgt gcatgtgccc cgcaggacat
     1981 caatgctggc ttctaccagc gctgcgccat agacatgata ggcaaagggt tttccacgct
     2041 ccgttttcat atcatattcg agtgtcggcg ttgcataaaa gccatgtgcg gataggtctt
     2101 gacgcgatgc gctcatggct tcgacaagtt gctcccagct aatatggctg ggtgttccat
     2161 ttagtagtac tgctccatct ttgatatcca ccttgtccag atcgaccata aggagctcag
     2221 cggctttctt caatagtctt cctcggattt ccaagcatgc aattcttgcc gccatgccat
     2281 tcatgtccga tcccgtactc gcggcggtcg gcacagtatt ggctactgtc aaagttgtag
     2341 tgcgttgaac cgtaactcga tctatcgaaa caccaagagt ttttgcggca attatggcga
     2401 tttttcgagc cacctgttgg cccatctcaa tagcccctgt cgagaccact accgatccat
     2461 cggtgtagat atgcactaga gctcccgctt gattcatctg tagcttggta aaacttatgc
     2521 caaatgctac aggaataagg ccaatacctt tcttctcaag tcgatgagag gcattgaatt
     2581 cggcaatttt ggcctttagc gcaggataat cgaccttgca gagaagccgc tcgaccgagt
     2641 cagacgcccg cactcgctcc atagtcatac cataataggt tacatcccct tctttgaaga
     2701 gattgttccg ctttatttcg acagcatcga ttcccatctt ctgtgcaagc gcatcgatag
     2761 cagattcgat cacgaagaat gcctggggcc ctccaaaacc tctgaacgcc gtgaaagagg
     2821 gcaagttcgt atggcacata tagccggtta cccgcacatt tggaatctga tatgcaccgg
     2881 ttgcatgcag cacagttcga gcaagaattg ctgccgatag atcgcaggtc gagccagaat
     2941 tctggtaata gtcggcctca aaagcaataa gtttgccact cgcatcggcg ccgagacgaa
     3001 aatccgtaga ataaggatgt cgtttgcctg tagcccgcat atcatccctg cggttgaggt
     3061 atatttcgac tggcttgccg gtgacccaag aagcaacagc ggccatgcaa gcccaatttg
     3121 ctccctgatc ttccttgccg ccaaatgctc caccgaggcg ccttgcctcg acttctacca
     3181 tgctcatagg caggcctagc acttgggcaa cagccttctg gacgcctgtc ggaccctgtg
     3241 tactcgaaat gacaagcact ctacgaccat ctatcacctg agcgattgcc ccctgagttt
     3301 cgaggtaaac atgttcttgg ccgccagaat cgactctgcc ttcgacgata aaggcgcatc
     3361 tcgaaaatgc ttcgcgcaca tcgccacagg ctattgttct tggcggaaag atgaaatctc
     3421 ctttggcagc cgcttccctt ggatctataa cttcgggaag gttttctccc tctatgcgca
     3481 caaggcttgc cgctcttctt gcaaggattc gagaatttgc caccacaatc gccaaaggct
     3541 ggccccaata gtcccactca ctctctggaa ggagcggctc gtcgggctta ttaaagccaa
     3601 tctgattagt tccggggatg tcacttgcca ggatgacccg tacagaagta tcgagctcga
     3661 gagcttcttc tgtatggatc ttgcgaattc taccatgtgc gctctgagat agaaccacaa
     3721 cggcctggag acaacccgct gggcgcgtaa tatcatctat gtattgagtt tgccccagaa
     3781 catttggcat tgattcgggt attaagcccg acgagccgct ttccaattct actttgcctg
     3841 cccagacttg gctcatgtcc gaatttgctt tccgggtcag agattcatct agctcaagta
     3901 tatctttatt gttcatggaa atagctcctc tgcgacaccg ctatcttcaa agatgcgcac
     3961 aaaatgtgca agcatgagcc tgtagaccat tctgcgccga tattcttcag atccgcgcac
     4021 atcacttatg ggccttaccg aagcctctgc cgagcttgca actctcttag caagctcagc
     4081 cattatcttc ccatccgaga cagggcagga tgagccttcg agtagctcca ttgctgcctt
     4141 attgactgtt ggtgtgggcg ctactcctcc aaggcttatg tgtacatcct taaacctttg
     4201 ctcttcgatt ctaaaagata tggccgtatt gacggcagcg atatcgagat tcttgcgctt
     4261 gctggccttt tcaaaggaaa atttccgcgc agtcggatcg ttacaggatg gaattctgat
     4321 tgctttgatg acttccgtgg gttttagatc tattgtctta tatcccaaaa agaattcttc
     4381 cagcttacaa gtgcgtgtca aagacaggtc aaagccctct tgtatctgag acgcaatgac
     4441 tatgcttgca ttgagagcta gcagcattgc cgttatatcc gctactggcg aggcatttac
     4501 gatatttccg ccaatggtgg cgagattgcg tatcagtgtg cttgcaaaca tcgtttcgaa
     4561 ttgctcgatt cctggaactt cttgtctaat aagctctgaa gcgaaaaatt ccctcaccgt
     4621 gacggcggcg ccgatttcga gccatctttg tccattctca tctaagtaat taatctcttt
     4681 gaattctggc ctcattctaa tcaagaatgg cgaaaagcca tcgtttggtt cggggttttt
     4741 cacataatag tctgtccctc cacctaagat aacaaagcca ggagatgtca ttctctgctt
     4801 gggcgttaat tccggattct ccttatcatt attagaattc aggaggactt gagtcgatgc
     4861 ttcatcggca aattcgcgga ctgacggtgg aagcacttga gcactcacca gtgcattcaa
     4921 tctagcctca ggctcaaggg gcaggtcttt gaattgctcg ataagcctgc ggactgctct
     4981 tcgaatcgcc gcataccccg tacatctgca caaattccca tctatggctc gcagtgctcc
     5041 agcaagatcc aatagccttg gctctgccaa ccacgaagtc aaggcgatga tgaatccggg
     5101 cgtgcaaaat ccgcattggc tggcattctc atccagaaac gcccgcataa ccggagtaag
     5161 acctcccgaa gtcccttcga ttaggccttc gagggttata agatgtttgc ctttgagttc
     5221 gccaagagcg agcaagcacg aagggactgc tagatatcgc agttggccgg ctgcatattc
     5281 tccgatcaat accgcgcatg cgccacaatc gccttctcga cagccttcct ttgttgccat
     5341 aagtccaagt tcgcctcgaa ttatgtcgat tgccggcgtg ccttcgtctg agcgaatatc
     5401 ctgccatgtg ttgttgacct ttattcgcaa acaagccatt ttactctacc tcttcaaaaa
     5461 ttataccata gatttttgag ataggcattt attacggtgg actcggctct ctgcatgcct
     5521 ttattcatgg aaatatgcaa gcttgtggaa agtacttgcc ttatatataa ggatataagg
     5581 ctgccaagat gccgaatcag tgctaatctt tgactgtttc gttcacgtag agcgatactt
     5641 tttcgaactt ctcgacatcc acaagacctt tatctgtgat tttcaggctt gggattactg
     5701 gaagagccat aaaggagaga gtcataaggg gagaagttgc tgtcaggcct tccttcagga
     5761 atagtctatc gaaatcgctc agcttttcta tcacaaagtc tgctggcttg tcgctcataa
     5821 gccctgcaac aggtaagggc agatccagca aaatctggtc tcccacggca tagaccaggc
     5881 cgcccttgat tttattgaga tgcctggctg ccttaaagat cgataaatca tcaacaccag
     5941 caataatcat atgatgcgaa tcatggctta tggtgctgcc tatcgctccg cgtttaagcc
     6001 cgagtccttg tataaagcct ttacctatgt ttcccgagcc cgtgtgcctc tctataacaa
     6061 atatcttcag gatatcgcgc tctaagtccg aaaccagtgc cccattttcc gaaggtagtc
     6121 gctccaaacc atgtatggtc agaagcgaat ttggctggac tctgattact cttacaggag
     6181 attgcttatc cggaatcacg aagtcttccg cagtcaacca tttgatgttg acagagtctc
     6241 tgattgctat tggctctgcc gaaaaatctt cgatcagtga accatctctt gctacaattc
     6301 ttccattttt gatgactgcc ttgatctcga agaattcaaa tgaagggaat aagataaaat
     6361 cagccttata acctggtgca attgctcctt gagcagggat tccaaaccat cctgccgcat
     6421 tactcgaagc tatgcgaatt gcatctatgg gatcgatacc cgcgtctagc ataagcctta
     6481 gcgagtagtc tatgtggccc tcatcgcgta gatcattgca atgcctgtca tcggaacaga
     6541 gcaggaagcg ccccgcattc tcgcctcgca ccgcggataa cagattacgc agatcttttg
     6601 ccgtggaacc ttccctcaac attatgaaca ttcccttgcc cactttttcc aatgcctctt
     6661 ctgcggtggt gcactcatga tcagagccta tgcctgctgc tatgtaggcc gatagacgag
     6721 cgccagatag tccgggtgaa tgaccatcga taggtcttcc attcgacttg aacagtgcga
     6781 tcttatccat aagttgtgga tcttgggcca aaactcctgg gtagttcatc acttcgccaa
     6841 ggccgagcac gcgaggttcc tgcaaaaact ggtgcatatc agaggcatag agcgccgcgc
     6901 ccgcagtatc gaaatcggtg gcaggtacgc aggatggcac cataaaatac acatcgaggg
     6961 ggatattctc tgaaacattc agcatatatc gcatgccatc atagcccatc acattggcaa
     7021 tctcatgagg atcggcaact acagttgtgg ttccatgagg tattacgacc ttggcatatt
     7081 ccgccggagt cagaagcgat gactctatat gtacatgggc atcgatgaga ccgggtgcaa
     7141 gatacattcc tttcagatct atagtttcct ttgccgaaag cccatcgttg attcctgcca
     7201 cataaccatt ttttatcgaa ataacagaat ctttgagcaa acggccggaa aggacatcta
     7261 ccaatataca gttggtataa ttaacgtcgc attgtgcctt tcccatcgcg gcctcgatta
     7321 tgtccttttt catttttcct ccctcatgta tgaggatcca agcgcggcgg gcgccccgac
     7381 tctcttggat aaggcttccc accatgtgat agcgacgaga accagtatgg tgccaatata
     7441 tggcaacatg ttgagaaagt ttgcaggtac gctcgttccg gatgcttgaa gacgaaactg
     7501 gacggcattg atccctccaa acaacaaaga tccccacacg gcgcgtgcgg gattccacat
     7561 cgaaaagata acgagtgcaa ttacgatcca tcctcgcccg ccggtaatgt tctccgacca
     7621 tcctggtgtg taggaaagag aaagatgagc accacccagg gctatcagca tgcctccgat
     7681 tattgtatag aagtacctgg tctttgttac atcgattccc atcgcatcag cggtataggg
     7741 atcttccccc acagaacgaa gccataagcc cggtcttgtc ttgtaaagga aaaaccatgc
     7801 aaggggaatg aaaagatata caaaataggt cattatatct tgattcaaaa aagccgaaag
     7861 aatcggaatg tcttttgccc aattcggagc aaagggcagg attcgctctg ctctaaggcc
     7921 tacaagtctg aaattgttgg attccggtcc aagcctttgt ccaagaaatg acgaaaatcc
     7981 tgttccaaat agcgttatcg aaagccctga gacgacctga tttgccctca tcgttgtggt
     8041 aagaaaagca tgcagcaagg cgagaatgcc tccgatcgca attgctgcta aaattcccag
     8101 catgatgctg tggctataaa aaacggtggc aaaagctgtt aaagccccca taagcatgat
     8161 tccctcgaca ccgagattca gaatacccgt tcgttctgtg agtatttctc cgatagttgc
     8221 aatggtaagg cttgttcctg ctcgaatggt aatcgttaga attgaagtga taaaatccat
     8281 gcattgctcc taagttgcat ctcggcaggc tgaatttatg ttgtctttta ctgccataag
     8341 tctgtattcg gtgaataagg atcccgcaag catcggaaat agtatgagac cttgcatcac
     8401 tgtacccatg gctgcgggta gccccattgt catctgcacc tgatcgcctc caacagatag
     8461 tgcggcgagt gctaccgcta caaaaggaac agcaagagga ttgagttggc tcatccaagc
     8521 aactattatt gctgtatatc cgtaacctat tgaaaggcct tgttgaaggc ggtgcgatat
     8581 acctgttacc tcgaatgctc cagcgagtcc gcagagagcc cccgagattg taattgcaag
     8641 aacaatattg cgttcaaccg cgattccctg atatcttgcc gcttgctggc ttgcaccgat
     8701 gatctgtagc tcgaagcccc aacgtgtgcg ctttagaata aaccatagaa caagcgcaag
     8761 gaacaaggca atcaaaacgc caatatgcgc gcgtcccact atgcgcggaa gccatgcatt
     8821 ggatgcaaac atacgcgaac cagggaatcc atatccctta ggatcacgcc atggaccata
     8881 gtacaggtat tcggagaaca gaatagctat ataattcatc ataagagtaa caagcgtctc
     8941 atcgacattg agagaagtct ttagaagagc tggtatacca gcccaaagcg caccggcaag
     9001 caagcctcct aatagggcgc ccactaagag taaaggaccc ggcagaaatt gttccataaa
     9061 aagagctact cccgctgccg ctacgcctcc caatgcaagc tgaccttcgg ctcctatatt
     9121 ccagaacttc aaccgaaaag cgattgccac tccaagacct gtcatcatta gaggaatagc
     9181 ctttacaagt gtctctgcaa aaccatacaa agatccaaaa gcaccaattg ccattgctgt
     9241 ataggttttt aaagggtcgg cttcaaagat cataagcaac agggcagtca gaaacagaga
     9301 gaccgcaaat gataccatag gcacgagtat aagagcggaa atcgatgttg tttttctttt
     9361 ttcgaataca agtctcatct gatggcctcc gcattcatgc ccgccataag catcccaata
     9421 ttttcgatat ctgctttttc agcgtcgaaa atatccataa accttccttc gaacattact
     9481 gctatgcgat ccgatacttg caggagttca tccagatctt cggatacaag cagaattccc
     9541 aggccttcat ctcgcatatt gatcagttga cgccttactg attctgtagc ccccacatcg
     9601 agccctcgcg aaggatagac agcaaccatc aaagatcgtg ctgcgccgat ctcccttgcc
     9661 aaaatcgtct tttgcacatt tccgcccgag agaaacttga tctgagtgcc gggcgatgga
     9721 gtggcgatgc gaaatttgtc tatcaatttg cgggcaaatt cgatgattct tttgggaagg
     9781 aggataattc cctgtgcgag aggcctggaa cgatattgct tcatagcaag attcgaagcc
     9841 acgctgagat ctcctacagc tcctacggat gccctatcct gaggaatatg ggctactcca
     9901 tgacaaatga tctcaagtgg agacttgttg gtcatatctc tgtctgcaat ctttagcgtt
     9961 ccttttgtgg actttcgcag tccggttatg acttccgcca gctctcgttg gccattacca
    10021 tcgaccccgg ctatgccgag tatttctcct gccctaaggc taaatgatat attcttgagg
    10081 gcaggcagtc ctctatcgcc aagcgcttca atatcggaaa gctccagtat tgtcttgccc
    10141 ggtttgtagg gctttctttc tatttgaaaa agaacttcac gtcccaccat cattcgggct
    10201 aagtctttag gactcgtctc cgaagtaggt gtttccgcaa caactctccc tttctgcaag
    10261 accatgacgc gatccgagaa ctcaataact tcttccattt tgtgagttat gaatattgcg
    10321 gatttgccct cctgcttcat ggtctttatg acgcgattca gttcatgagc ctcttgtgga
    10381 gtgaggaccg cggtgggttc atccagaatc aggacttcgg catttctgta gagaagtttt
    10441 aggatctcga ctctctgctg ttccccaaca gatagctgcc agatataggc atccgggtcg
    10501 atattgagat gatattgaga tgctaattcc attagctgct tgcggactcg tttcatatcc
    10561 ggaatgaatg gctcatcgcg gaggcctagg atgatgttct caagaacatt catcgaattt
    10621 acaagcttga aactctgatg caccatccct attccatatt tctgagcatc tcgaggagag
    10681 tggatctcga ctttctgtcc atctatgtat atttcacctt gatcttgcct atacaagcca
    10741 cacagtatat tcatcaaggt gctttttcca gctccattct ctccaagaag cgctaagact
    10801 tctcccttat ggagtttcaa atcgatgcaa tcatttgcaa cgactcctgg gaaggacttg
    10861 gagatccgtc gcatttcgat tctcggaatg ttcgaatcaa tcatagattt tatggataga
    10921 gaaagccggt atgtattgca tagagcacac ataccggcaa taaagaatca ctgaacctta
    10981 cctactacgc cttctacaaa ccaatccatc gagagttgct tgtcatctgg aatgatttcg
    11041 ccggccttat agacaatttt gccattttga tcctttatgg ggccggtaaa gatgctccag
    11101 ccgcccaata tcttcttctt tgcattctcc acttcttttc taacattggc tggcacgagc
    11161 ggactcagat cggagaggct gactatatca tcttttaatc caccccagta ggaatgtgtc
    11221 ttccaggttc cgtccaatac ttcttggata gtccttatat agtaggttcc ccagttccaa
    11281 attgcactgg caagaacagt atctcctacg aattgtgcca tatctgagtc atagccaata
    11341 gatagcttac ctctctcctg tgctgctttc tggggttccg tagtatcctg gtgttgtgca
    11401 atgatatcgc agcctgcatc gagaagtgct attgcggctt ctctttcttt tactggatca
    11461 taccaggtat ttgtccagac gacctggatc ttggcattag gattgacaga gcgtactccc
    11521 agagcgaagc catttaaacc tcttacaact tcaggaattc caaaggctgc tacatatccg
    11581 atcttattgg tcttggtcat gcggccag
//
LOCUS       DAOMZL010000052        11493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_5272_length_11493_cov_17.209302, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000052 DAOMZL010000000
VERSION     DAOMZL010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 11493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11493
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5272_length_11493_cov_17.209302"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            950..1978
                     /locus_tag="PLQ30_05495"
     CDS             950..1978
                     /locus_tag="PLQ30_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPW01934.1"
                     /translation="MNKQKISIHEVARLAGVSKSTVSRVISEKGGSVSQETAKRVHTA
                     IDQLGYRKNIVAAGLRTKTTYMVLLMIPDVANPFWSEIARAVQDSLEPEGYSVVIGST
                     DFSEEREARYYELARMSRFDGVILNSVTDNIDLIKNLGIPSVLIGERTAAQHIDTVGT
                     DTLQATKIGMEYLYETGHRKIALATSDHGSERYLSLRARAYKDFLHEKALIFDPSLLF
                     SVNLNEEGGKELARKLLTINNWKNQIDSLFCGNDIVAISAMNELRKFGIIAGVDISII
                     GMDDIPASSQTYPPLTTVRKPRQLIGVAAAHLLLKRIANPQDSTEKHLFPGELVIRDS
                     VINRRKGK"
     gene            1975..2970
                     /locus_tag="PLQ30_05500"
     CDS             1975..2970
                     /locus_tag="PLQ30_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPW01935.1"
                     /translation="MKSRADILAQREIFESRAIGAFIGLAVGDAVGDLGRNQEYRNRY
                     GLITRLFEDGKSTDDTEFGVLTARMLLDAKANLTPQKTANAWRHYILERGGAKKRAGR
                     PLYGAIENLSRGMEPPLSGRFNVMNIDDGAAMRAIPHGILFAGNPSRAAASAVADACV
                     SHDADGIWAAQAVAAAIAVAIADGTTEEIVHTARSMLPLGSWIAHAMDIAMSICERAQ
                     NIEDAYEDLHIKLWTPEHATAAEAIPQVFAIFKLCNGEPHHSLLWSANFGRDADTISG
                     LVCSFSGAMHGDTAFPNEWIERLRKPSGTCLEFAMEEDIVQLAKDLVALSCSLEE"
     gene            2975..3865
                     /locus_tag="PLQ30_05505"
     CDS             2975..3865
                     /locus_tag="PLQ30_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPW01936.1"
                     /translation="MHSLSKKKDWGLVLLLAPALIYFLVYHIIPILGMSMAFQDYRII
                     GKSPFVGLKHFRILFSSPAFFQVLRNTLIISFMKIILYFPLPIIFALMLNEVRSGFIR
                     KFIQSTTYLPHFLSWVVIAGIWIAFLSPTNGGFNQLRGFFGLPPHDYMTDKSAIRWVL
                     FASEAWRSMGWDSIIYLAALIAISPSLYESADLDGASTLQKMRFITLPELLPTMVTVF
                     ILNMGFFLNAGFDQVFNMMNDSVISVIDILDTYVYRIGLVNMQYSFSTAASLFKGVIG
                     LMLIVSAHCGSKKITGKGLW"
     gene            3859..4776
                     /locus_tag="PLQ30_05510"
     CDS             3859..4776
                     /locus_tag="PLQ30_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPW01937.1"
                     /translation="MVKDKYPPKKVRIPRAELFSRWCFTILLLLASILVLVPLLNLLA
                     LSLSDPSRVSEVRGLTIVPKGFSLINYKVLYANPLFMRSILNSVFITIVGTALNLIVT
                     SMAAFALTRPKLPFKRFFMVLTIVIMILEPPLVAEYLVIKKIGLMGSLWAVILYKTVN
                     VYYLIIMMRYFEEIPSSLIEAAEVDGATNMTILRNIVFPLSKSAFATIGLFYGVFHWN
                     EYFRASIYLTDPMKYPLQLILRQFVVLDDTASLIGSGALYSYNEAARLSYNALQASTI
                     IVAILPVLIIYPLVLKYYTRGVMEGGVKE"
     gene            4786..6210
                     /locus_tag="PLQ30_05515"
     CDS             4786..6210
                     /locus_tag="PLQ30_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPW01938.1"
                     /translation="MKKNKSMLMFAVAIAILGCAVAGAQPKVRLVLKDLDTDPNTPQF
                     IQLIEKGMAAAGTPVKIEVVKVPSGSYAEKVSLMITSGDIPDLIYFQGSDEKIAMQGL
                     LEDLRPYVNKSKYIKNVLDPHSKTRLENYPYLVWVAPPRVRVPVIRKDWYDSLPSAKA
                     LVAKPTIDAYYALFKDLVQKKGAQYGISITGATNGAEELDSIFDAAFGITSTWMKGSD
                     GQWVYCRVTPNEKEKLTFYAKLYKEGLLDPEYLTKKWDTKEQAFYEGKAGMVAGVAPS
                     SVDVYANKMMKTQNTTIVPLPPPTGKSQGLGPFVDVIKESRGFAIPVTSKVKDVAFEV
                     LDFMWGPAGLKIAKLGIPGVHYIEEPNRYVLTDRYPEWVNGWFGDSFNGFNPDKPLSR
                     PIMTDAAVEAGKLAVMYMMPDKTFLIPEQYITNWDAMSNLYKEYEADIITGKKPISAF
                     DEFAQKWNRAGGTELTKYAQTVLK"
     gene            6361..8553
                     /locus_tag="PLQ30_05520"
     CDS             6361..8553
                     /locus_tag="PLQ30_05520"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPW01939.1"
                     /translation="MASFIPDDYVERVYAGLLGKNIGIRLGAPVEPAIWTYQRIGEIY
                     GDITSYLKAYRNFAADDDANGPVYFVRPFLEKVEEMLKHEPQEARLGDMQLEAQEEGR
                     AWLNYAREGVGMFWWGGYGRSTEHTAYLNLKNGIPAPESGSVRRNGKVVAEQIGGQIF
                     VDTWGLIWPGNPKKASEFAARAASVSHDGEGLNGARFIAASIAAAFDAADVLSIVDTG
                     LGLIPQDSAYASVVQAVKQFHSLYPADWRQCQEYLISEWGYDRWPGLCHIIPNAGVCA
                     LALLYGEGSFARTVEIATMCGWDTDCNAGNVGTIAGVLYGLDSLPEHYRKPINDSIVL
                     SGLPGVLNVLDIPTFADRLARVGYAMSGESIPNYLVESLWKGDGEQALCFDFQLPGST
                     HGMRASDAIRFATGHREIEHNGINRGALEFRFERLTREESGTCYFKPYYRRSDFDDER
                     YSPVFSPLARPGQHVDISFCLEQWEGQRIGIEGYVRDTWSRKEIITTPTLFFDMGKDM
                     TGQPKEEYRVSFDIPSMQGSFPDEVGLKVTSFSGKGKRDSGRLFVLQFTLSGKASYDI
                     DFSKQTIEFGSVTPFSHEGGTWSIENNRLHLMTPTRAAAYTGGYRVANQKVEATVTPL
                     AGDSHMLLVRAKGAQQAYWGGIDDKGKLAVYRQNFGFEKLAETDFAWDHDTDIQMTLS
                     AIGDRISVRIDDEKLLEVRDNELRSGMVGCGSLRGSRTLWGPFAIEEL"
     gene            8569..9816
                     /locus_tag="PLQ30_05525"
     CDS             8569..9816
                     /locus_tag="PLQ30_05525"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HPW01940.1"
                     /translation="MSNSEQSEAGFLSKSDCIVRYEPSQEPLSIEVRSSVRALFETQI
                     RQCVLRIFQEFDVTKGRIAIEDDGALDLVISARVEAVLRQAGYLSQMKTMSPEKGKYG
                     VSLVSSNGAGFTQSSRRVRLYMPGDQPHFAVNADFYGADILIFDLEDSVAQSRKCEAR
                     ILVRRFLENSFLFRKSARAVRINPLSTEESMLDLAEIVPAKPDIVLLPKCETADDIKR
                     LDALLSEHENRCGTMPGGIQIMPIIETAKGVLAARGIACASKRTSGLCFGYEDFSRDI
                     QSSSDILGKALLMHSGSMHQIDSLLARETIVLAARAAGIEPYDSSYGDIDNTEGLLMS
                     CYEARALGFAGKSVIHPSQIPYVRKAFMPTAEELHQAQAIVAAYEESFSTGKGAIVVD
                     GQMVDIPVVEKARRIIEEFDYGE"
     gene            9806..11386
                     /gene="citF"
                     /locus_tag="PLQ30_05530"
     CDS             9806..11386
                     /gene="citF"
                     /locus_tag="PLQ30_05530"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HPW01941.1"
                     /translation="MGNDTTWTDNALGRKVPSRIAGKPCRSFLGAFIDSTQAKHEKTK
                     NGQLRRSSGMTQRQKPNKLVASWDELFERLNIQDGATISFHHHLRNGDRIVNEVVGRL
                     AAKGVCDLTLAPSALFPVHNALLPYIRSGVITKIEGSMNGTIGAACSRGEIPKTCVLR
                     SHGGRQRAIQEGDLPIDVAFIAAPCADSFGNANGLYGPSACGPLGYAKPDSEYAHQVV
                     VITDNLVPYPCSSWSIFGGNVDYVLQVESIGDSSQIVSGTTLLTKSPTQLVIARLAAR
                     FIEAAGIMQEGFSFQAGAGSISLATIVFLAERMRKAGVKASFAHGGATKVLVDLLKEG
                     LLGSIVDLQSFDLDAVRSCAENARHIESDPYSSYGPYARGCVANMLDVAVLGATEIDL
                     DFNVNVVTHSDGMLLHGIGGHSDVCSSAGCTIITAPSFRKRIPIIKEQVTTITCPGEA
                     VDVLITERGISINPNREDLIWRAKKFDLPCVDIAQQMNDVYRITGKPEKPRFTELPVA
                     LVEWRDGSIIDTVWQMAE"
BASE COUNT         3162 a         2432 c         2780 g         3119 t
ORIGIN      
        1 acttttcgcg agccaagaat cgaagcaagt cacgatccaa ttcgtacatc cgaagattcc
       61 attgcataga gtaaatatcg cacactatca taaaactgta gataaactga ccaaattatg
      121 ccaaatttag tcagtttgat gacaatttta tagaattgcc aattcaagtc tgaagtacaa
      181 aaaggataaa tgtagaaaaa tggcattaga gaatttggta ggatgtgaat atcacacaga
      241 catccatacc ggaagtcaca aatgacatgt cagtttttgc aagtcatgag caagatcagt
      301 ttcaggttct gatctcgggc aaaccataaa agtgaggtat ggctaggaca cttgaggaac
      361 atgtctttta tagctacaga gagatcgtaa ggaaccagaa agagggctac catttcgccc
      421 atcatgcata cactacagca caacagcgtc gactaaaacc aaccttcagt ataaaacgag
      481 cgaatataga gttaatgcat aaggtttagt aaaggattct caacgagcac tcttccatca
      541 ggggaataat gaacatcgaa aacgccagtc agacaaatat aaacgagtgg ttatatacaa
      601 tcggtgcttt attgatgaat gtgctcaaat tgtaaatttg aagaatagct tcggtgactg
      661 agaggagata atatcgagaa aagttttctt taatctccct tttcaacctc agatttaaag
      721 tagtctcact taaagagctt taaaagagct cagattgttt gttatcttaa aacattcatt
      781 aaacttgagc tttttatttt gattctaata tatccaaaaa ttcaaaaaat attgaatgcc
      841 tcatttcttt cttataatta gcttagtact ataaatatag ggaacgttcc caattatgac
      901 ttgcatcttc agaaaggtgt gttataatac taatgggaac gataccaata tgaataagca
      961 aaagattagt attcatgaag tggcgcgtct agcaggggtc tcgaaaagca cagtctctag
     1021 agtgatttcg gagaagggcg gatctgtcag ccaagagaca gccaagcgtg tacatacggc
     1081 aatcgatcaa cttgggtatc gcaagaatat cgtagcagca ggcttaagga caaagacaac
     1141 atatatggtc ctgctcatga ttccagatgt agcaaatcca ttctggtcag agattgcgag
     1201 agcggtccaa gattcgctcg aaccagaagg ttattcggtg gttatcggta gcactgattt
     1261 tagtgaagag cgagaagcac gttactatga gctcgctcga atgtctcgat ttgatggggt
     1321 cattcttaat agcgttactg ataatatcga tcttatcaag aatctgggta taccctcagt
     1381 cctaattggt gagcgaactg ctgcacagca tattgatacg gtagggaccg atacccttca
     1441 agcaaccaag attggcatgg aatacctcta tgaaacaggt catagaaaaa tagcccttgc
     1501 aactagtgat catggtagcg agcgctatct ttctcttaga gcacgagcat ataaagattt
     1561 tcttcacgaa aaagctctta tttttgatcc ctctcttcta ttctctgtga atttgaatga
     1621 agaaggtggt aaggagctag caagaaaatt gctcaccatc aataactgga aaaatcaaat
     1681 cgacagcttg ttctgtggca atgacattgt tgcaatttct gcaatgaatg agctcaggaa
     1741 gtttggcatt attgcgggag tggatatttc gatcataggt atggatgata tccctgcatc
     1801 ttcccagaca tatcctccgc tgacaaccgt acgcaagcct cgccaactca ttggtgttgc
     1861 tgctgcacat ttattgttaa aacggatcgc gaatccgcag gactcaacag aaaaacatct
     1921 tttccctggt gaacttgtga tcagagatag cgtaataaat agaaggaaag gaaaatgaaa
     1981 tcacgagcag acattcttgc acaacgagag atatttgaat caagagctat tggagctttt
     2041 attgggctgg cagtcggaga cgcggttggc gatcttggga gaaatcagga atatcgtaat
     2101 cgatatggtc ttattactcg tctgtttgaa gatggaaaaa gcaccgatga taccgaattc
     2161 ggtgtactta ctgcacgtat gctactcgat gccaaagcaa atctcacacc ccagaaaact
     2221 gcaaatgcat ggcgccacta cattcttgaa agaggtggag caaagaagag agcagggcga
     2281 ccgctttatg gtgcaataga gaatctcagt cgcggtatgg aaccgcctct atctggcaga
     2341 ttcaatgtaa tgaatatcga tgatggtgca gcgatgcgag caataccaca tggcattctc
     2401 tttgcaggaa atccttcgcg cgctgctgct tccgcagtag ctgatgcatg tgttagtcac
     2461 gatgccgatg gtatttgggc agcgcaagct gttgctgcag cgatagcggt agccattgca
     2521 gatggaacca cagaagaaat tgtacatact gcgcgatcga tgttgccact tgggagctgg
     2581 attgcgcatg caatggacat tgccatgagc atctgcgagc gcgctcaaaa tatagaagat
     2641 gcatatgagg acttgcatat aaagctttgg actccggagc atgcaaccgc tgctgaagca
     2701 attcctcaag tgtttgcaat tttcaagctt tgcaatggtg agccccacca tagtcttctt
     2761 tggagcgcaa attttggaag ggatgcagat acaatttctg gtctggtttg ctcattctca
     2821 ggcgcgatgc atggcgatac cgctttccca aatgaatgga ttgaaagatt gagaaaaccg
     2881 tctggaacct gtctcgaatt tgccatggaa gaagatattg tgcagctcgc aaaggattta
     2941 gttgctttat cttgcagtct agaggaataa gagtatgcat tcgctatcaa agaaaaaaga
     3001 ttggggcctt gtacttttgc ttgctcccgc actgatttat ttccttgtat accacattat
     3061 tcccattttg gggatgtcaa tggcttttca ggattatcgt attattggca aaagcccttt
     3121 tgttggactt aagcattttc gcatactttt ttcgtcaccg gcattcttcc aggtccttcg
     3181 caacaccctc atcatcagct ttatgaaaat tatattgtat ttccccttac ctataatatt
     3241 tgcacttatg ctgaatgagg tccgttcagg atttattcgc aaatttattc agtccacgac
     3301 ctatttaccc cactttctct cttgggttgt tattgctggt atatggatag cttttctttc
     3361 acctacaaat ggtgggttca atcaacttag aggatttttt gggcttccgc cacacgatta
     3421 catgacagac aagtctgcta tccgatgggt gctgtttgct tctgaggcat ggcgcagcat
     3481 gggatgggac tcgatcatat acctagctgc attgattgcg atcagcccaa gtctctatga
     3541 gtctgctgat ctagatggtg caagtacttt gcagaaaatg cgttttatta cacttcctga
     3601 gctccttcca acaatggtga ctgtctttat tctcaatatg ggcttttttt tgaatgcagg
     3661 gttcgatcag gttttcaata tgatgaatga ttctgtaatc agtgtgatag atatccttga
     3721 tacctatgtg tacagaattg gccttgtcaa tatgcaatat tcattttcaa cagcagcgag
     3781 tctttttaag ggcgttatcg gccttatgtt aattgtgtct gcacattgtg gatcaaaaaa
     3841 aataactggg aaaggtttat ggtaaaagat aaatatcccc ctaaaaaagt gcgaatacct
     3901 cgagcagagc tcttttcgag atggtgcttt acaatcctat tgctattggc atctatcttg
     3961 gtgcttgttc cgttgctcaa tttgcttgcg ctttctcttt ctgatccttc aagagtctca
     4021 gaagttagag gacttacaat agtgcctaag ggcttttcgc ttatcaacta taaggtactc
     4081 tacgcaaacc cactttttat gcgcagtatt ttaaattcag tctttatcac cattgtagga
     4141 acagcgctga atcttattgt gacttccatg gcagcttttg cattgacaag acctaaactg
     4201 ccatttaagc gctttttcat ggtgcttact attgtcatta tgattctcga acctcctctt
     4261 gttgcagaat atcttgttat caaaaaaatt gggctaatgg gcagcttatg ggcagttatc
     4321 ctctataaaa ctgtcaatgt gtattatctc attattatga tgcgatactt tgaggaaatt
     4381 ccttcttctc ttatagaagc tgcagaagtc gatggtgcaa ccaatatgac aatcctaaga
     4441 aatattgtat ttccattatc aaaatctgca tttgcaacaa taggactttt ctacggagtt
     4501 ttccattgga acgaatattt tcgcgcaagc atatatctca ccgatccaat gaaatatcct
     4561 cttcagctta ttctacggca atttgtagta cttgatgata ctgcgtcgtt aatcggttcg
     4621 ggagcactat atagttacaa cgaagctgca aggcttagct acaatgcact acaagcgagt
     4681 accatcattg tagcaattct accggttctc attatctacc ctctagtcct caaatattac
     4741 acacgaggag taatggaggg gggagtaaaa gaataggagg ttcttatgaa aaagaacaaa
     4801 tctatgttaa tgtttgctgt tgcaatagca attctcggat gtgcagttgc gggagcacaa
     4861 ccaaaggttc gtttggtcct taaagacctc gatacagatc caaatacacc acagttcatt
     4921 caacttatcg aaaagggcat ggctgcagcg ggcacgcctg tgaagatcga agtagtcaag
     4981 gtaccatctg gtagctatgc ggagaaggtg agcctaatga tcacgtcggg agatattcca
     5041 gacctcattt atttccaagg cagcgatgag aaaattgcca tgcagggctt gcttgaagat
     5101 ttaagaccat acgtgaacaa gtcgaagtac ataaagaatg tactcgatcc tcatagtaaa
     5161 acaaggcttg aaaattatcc ttaccttgta tgggttgctc ctccacgcgt acgcgtacca
     5221 gtgataagaa aagattggta cgactcgctg ccttctgcaa aagcgctcgt tgcaaaacct
     5281 actatagatg cctattatgc gcttttcaaa gatctcgtac aaaagaaggg tgcacagtat
     5341 ggcatttcta taacaggcgc aaccaatggt gcagaagaac tcgattccat ttttgatgca
     5401 gcctttggta tcacttctac ttggatgaag gggagcgatg gtcagtgggt ttattgtaga
     5461 gtaacaccca atgaaaaaga aaagcttaca ttttatgcaa agctttataa ggaaggtcta
     5521 cttgatccgg aatatctgac caagaaatgg gacacaaagg agcaagcgtt ttatgaaggg
     5581 aaggcaggaa tggtcgctgg tgtcgcgcca tcaagcgttg atgtctatgc aaataagatg
     5641 atgaaaaccc aaaatacaac gatagttcct ctccctcccc cgaccggtaa gtcgcaaggt
     5701 cttgggccat ttgtggatgt gattaaggaa tcccgtggtt ttgcaatacc agttacttca
     5761 aaggtaaaag atgtagcgtt tgaagttttg gactttatgt ggggtcccgc tggtcttaag
     5821 attgctaagc ttggtattcc aggtgtccat tatattgagg agccaaatcg gtatgtattg
     5881 acagacagat atcctgaatg ggtgaatggc tggtttggcg attcattcaa tggttttaat
     5941 cctgataaac ctctttcccg tcctattatg accgatgctg cagtagaagc aggaaagctc
     6001 gcggtcatgt atatgatgcc tgacaaaaca ttccttattc cagaacaata tattacaaat
     6061 tgggatgcga tgagcaatct ctacaaggaa tatgaagcgg atatcattac tggcaaaaag
     6121 ccaatctcag cattcgatga gtttgctcaa aaatggaatc gagctggcgg tactgaattg
     6181 acgaagtatg cccagactgt gctgaaatag cacatcgtca tgtccgatat aatgaagaat
     6241 cgtttaacag aattgagagt atgagttttg gggcacaggc atcgccgcga ttgagtagca
     6301 agagccaaaa agcacgaaga tgctctgtgc cgctattaaa taaggcaggt atctacattt
     6361 atggcctcgt ttatcccaga tgattatgtt gagcgcgttt acgcaggttt gcttggtaag
     6421 aatatcggga ttcgtcttgg tgcgcctgtt gaaccggcaa tttggacata ccagcgtatc
     6481 ggtgaaattt atggcgacat tacatcgtac ctgaaggctt atcggaattt tgccgccgat
     6541 gacgatgcaa atgggccggt atattttgtt cgccccttcc tggaaaaggt agaagagatg
     6601 ctaaagcatg aaccgcagga agctcgacta ggcgacatgc agctcgaagc gcaagaagaa
     6661 ggaagagcgt ggctcaatta tgcccgcgaa ggcgttggaa tgttttggtg gggaggatat
     6721 gggcgcagta ccgaacacac cgcctatcta aatctcaaga acggaatccc tgcccctgaa
     6781 tctggatcgg tacgcaggaa cggaaaggtc gttgccgaac aaatcggtgg ccaaattttt
     6841 gtagacacct ggggtttgat ctggcctggt aatccaaaga aggcctcgga atttgccgcg
     6901 cgcgcagcct cggtctctca cgacggagaa ggcctgaatg gtgcccgttt tattgcagcg
     6961 agtattgccg ccgcttttga tgcagctgat gtgctctcta ttgtggacac tggccttggc
     7021 cttataccgc aagactcggc ctatgcttcg gttgttcaag ccgtaaaaca attccattcc
     7081 ttgtacccgg cagattggcg acaatgtcaa gaatatctca tctccgaatg gggatacgat
     7141 cgatggccgg ggctctgtca cataattccc aacgcaggag tttgtgctct tgcacttctt
     7201 tatggcgagg gtagttttgc gcgcaccgtt gaaatcgcga cgatgtgcgg ttgggatacg
     7261 gactgtaatg caggtaatgt gggtactatt gcaggagtgc tttacggcct cgattcgttg
     7321 cctgaacatt atcgtaagcc aataaacgat agcatcgtgc tgtctgggct gcctggtgtt
     7381 ttgaatgttc tcgatattcc tacatttgca gatcggctcg caagggttgg ctatgccatg
     7441 tccggagaga gcatcccgaa ttaccttgtg gagtcgttgt ggaagggcga tggcgagcaa
     7501 gcgctttgct ttgatttcca gcttcctggt tcgactcatg gcatgcgagc ctccgatgca
     7561 attcggtttg caactgggca tcgagagata gaacacaatg gtataaaccg aggggccttg
     7621 gaatttcgct ttgaacggct tacacgcgaa gaaagtggaa cttgttattt caagccgtat
     7681 tatagacgat ccgattttga tgacgaacgt tacagccctg ttttttctcc gctggcaagg
     7741 ccaggtcagc acgtagatat aagtttttgc ctcgaacaat gggaaggtca acgtattgga
     7801 atagagggct atgtgcgcga cacctggtct cgcaaagaaa tcatcacgac gcctactctc
     7861 tttttcgata tgggaaaaga tatgactggc caaccgaaag aggaatatag agtttctttc
     7921 gatattccgt cgatgcaagg gtccttccct gacgaagttg gtcttaaggt cacgagtttt
     7981 tcgggcaagg gtaagcgtga cagtgggagg ttattcgttc tccaatttac cttgagtgga
     8041 aaagcaagct acgacatcga tttttcgaag cagacgatag agtttggttc tgtgacgccg
     8101 ttttctcatg aaggcgggac atggtcaatt gagaacaata ggctccatct tatgacgcct
     8161 acaagagcag ccgcgtatac aggcggttac agagtggcaa accaaaaggt tgaagcaacg
     8221 gtcactcctc ttgcagggga ttctcacatg ctattggtaa gggcaaaggg cgcacaacag
     8281 gcgtattggg gcggcatcga cgataaaggg aaacttgcag tttatcggca aaactttgga
     8341 tttgagaagc tggccgagac tgattttgca tgggatcacg atacggacat ccaaatgacg
     8401 ctgtcggcga taggcgacag aatctccgtt cgtatcgacg acgaaaaact gctagaagtg
     8461 cgcgataatg aattgcgaag cggaatggtc gggtgcggta gtctgagggg atcgcgaacc
     8521 ctctggggtc cgtttgcgat tgaagaacta tgagtaattc aggacaatat gagtaattca
     8581 gaacagtccg aagcgggctt tctttcaaaa tcggattgta tcgttcggta cgaaccttcc
     8641 caagaacctt tgtcgattga agttcgttcg agtgttcggg cactttttga gacgcaaatc
     8701 agacagtgcg tcttaagaat attccaagaa ttcgacgtaa caaaaggaag aattgcgata
     8761 gaagacgatg gtgctctcga ccttgtcata agcgcaagag ttgaggcggt gcttcgacaa
     8821 gccgggtacc tgtcgcaaat gaaaacaatg tcacctgaga agggaaaata tggcgtttct
     8881 cttgtctcct cgaatggtgc cggttttact caatcgagta ggcgcgtgcg gttgtacatg
     8941 cctggagatc aaccgcattt tgcggtaaat gccgatttct atggtgcgga catcttgata
     9001 ttcgatctcg aggattcggt ggcgcaatct aggaagtgcg aagcccgcat acttgtacgg
     9061 cgattcctgg agaattcttt tctctttagg aagagcgcac gggctgtaag gataaatcca
     9121 ctatcaaccg aagaatcgat gctcgatctc gcggagattg tccctgcaaa gcctgatatt
     9181 gttctgttgc caaagtgcga aacggccgat gatataaagc gcctagatgc gctcctttct
     9241 gagcacgaga atcggtgcgg aacaatgcct ggcggcattc agattatgcc gatcatcgag
     9301 accgcaaaag gggtgcttgc agccagggga attgcctgtg catcaaaacg aactagtggg
     9361 ctttgttttg gttatgaaga tttttcgcgc gacatccaat ccagtagcga catactaggg
     9421 aaggccttat tgatgcactc cggttctatg catcaaatag attcgcttct cgcgcgggaa
     9481 acaatcgtgc tcgctgcacg ggcagcaggt atagagccct acgattcctc ttacggtgac
     9541 atagacaaca ccgaagggct tcttatgtct tgctacgagg cgcgagcact tggttttgcc
     9601 ggaaaatcgg tcatccatcc ttcgcaaatt ccttatgtaa gaaaggcttt tatgcccacc
     9661 gccgaagagt tacatcaggc acaggctatc gtggcggcat acgaggagtc gttctcgacc
     9721 ggcaagggag cgatcgtcgt cgatgggcaa atggtcgaca ttcctgtcgt ggaaaaagca
     9781 agacgcatca tagaggagtt tgactatggg gaatgataca acatggacgg ataacgcgct
     9841 cggcagaaag gtaccatctc gcatagctgg caaaccgtgt aggtcctttc tgggtgcgtt
     9901 catcgacagt acccaagcca aacatgaaaa gacgaaaaat ggacagttgc gacgatcaag
     9961 cggaatgact caacgtcaaa aacccaacaa gctcgttgct tcgtgggatg aactcttcga
    10021 acgtctcaat attcaagacg gcgccaccat ttcttttcat catcacctaa gaaatggcga
    10081 cagaatagtc aacgaagtag ttggtcggct cgctgcaaag ggtgtctgtg acctaacact
    10141 ggcgccatcg gcgctttttc ctgtacacaa cgcccttttg ccgtacataa gatctggcgt
    10201 cattaccaaa atcgaaggct ctatgaatgg taccatcggg gccgcttgct cgcgcggcga
    10261 aatacccaaa acctgcgttt tgcgctctca tggcgggcgt caaagggcta tccaagaggg
    10321 ggaccttcct atcgacgttg cgtttatagc agcgccttgt gccgattcgt tcggcaatgc
    10381 aaatgggctt tatggtcctt cggcttgtgg accgcttggc tatgcaaaac ccgactctga
    10441 atatgctcat caggtcgtgg ttatcaccga caacttggta ccgtatccat gcagctcatg
    10501 gtcgattttc ggcggcaatg tcgattatgt cctccaagtt gagagtatcg gcgacagttc
    10561 gcaaatcgtg tcggggacaa cactgctgac aaaaagtccc acacagctcg ttatcgctcg
    10621 gcttgccgcg cgatttatcg aggccgcagg tattatgcaa gagggttttt cgttccaagc
    10681 gggagcggga agcatatcac ttgcgacgat cgtctttctt gccgaaagaa tgcgaaaggc
    10741 cggcgttaaa gcatcatttg cacatggtgg ggcaacaaag gttcttgtcg atcttcttaa
    10801 agagggattg ttgggttcga tcgtcgatct tcaaagcttc gatcttgatg cagttcgatc
    10861 ctgcgcagaa aacgcaagac acattgagtc cgacccatac agctcgtatg gtccctatgc
    10921 gcgcggctgt gtggccaata tgttagatgt cgctgttcta ggcgcaactg aaatcgatct
    10981 tgatttcaac gtcaatgtcg ttacgcactc tgacggcatg ctcttgcatg gtattggcgg
    11041 tcatagcgac gtatgcagtt ccgcgggatg caccattatc acggcgccat cttttcgcaa
    11101 gagaattcca atcattaaag agcaagtcac gacaataacc tgtcctgggg aagcggtcga
    11161 tgtattgata accgagcgcg gaatttccat aaaccccaac cgcgaagacc tcatctggcg
    11221 cgcaaaaaag tttgatttgc catgcgttga tattgctcag cagatgaacg acgtatatcg
    11281 gatcacgggc aaaccagaaa aacctcgatt taccgagctg ccggttgccc ttgttgaatg
    11341 gcgcgatggc agcataatcg acacagtgtg gcaaatggca gaataacctg atttcccgct
    11401 ttttgtaact aggtcgacga gggaatttca atacctaata atgaaagaat ctcgcgttga
    11461 ggctttgtga cttcagtaat gatcgagcca tat
//
LOCUS       DAOMZL010000053        11411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_5315_length_11411_cov_19.130680, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000053 DAOMZL010000000
VERSION     DAOMZL010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 11411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11411
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5315_length_11411_cov_19.130680"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..649)
                     /locus_tag="PLQ30_05535"
     CDS             complement(107..649)
                     /locus_tag="PLQ30_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPW01942.1"
                     /translation="MPSGLLSRIQKISQQGSDALISSQVSTDFPGCILARFVGAVTEA
                     NLDAVAQSFDSILSEGIRYLVVDFSTIEDIGPAGMGLMLALRQKLRDRQGDLVLCGMR
                     PRMERMERILGLEGYFTTATDAKSALTGLKFALNGIYPLSVQCPACNSLIDIEKPGRG
                     RCQTCEAVITAFPDGTITLG"
     gene            complement(742..1533)
                     /locus_tag="PLQ30_05540"
     CDS             complement(742..1533)
                     /locus_tag="PLQ30_05540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW01943.1"
                     /translation="MNNNNSEDIQGSIQLQNRVSTFSERLALVIGGSGGIGLAVSYEL
                     ASRGAALLIQGRRASEKLRVDSFPKAKSLNLIDLDFSSPVQFIQAIDLRLDEIGKEPD
                     IVVCAFGPFLEKPLKDCTAIDWEWLTLANLALPGALASRFLPGMMERKYGRFLFFGGT
                     RTDGIRSYKKTATYASSKTGLGVLAKSIAVQSASDNVAAIVVCPGPVETEYLDRETKI
                     RHASLTATGSLLKANIIAKIALDLIDSEPCIASGAVVNLDGGFAP"
     assembly_gap    1600..1699
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1786..2919)
                     /locus_tag="PLQ30_05545"
     CDS             complement(1786..2919)
                     /locus_tag="PLQ30_05545"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPW01944.1"
                     /translation="MKRLIYFDQDFRRIRPIIRLFSKYFDISVAGDLLELSIMIQRQK
                     PEILIVGLISSDSDFNARQKEIMKKIVPSSLETYYFHDYLELNDIVASVSEKSDKEHL
                     PSCGDKASGAKPRHADSSLNIIGKSKKIGHLISQIRMLANESCSVMIIGETGTGKELA
                     ARALHEWSDRRSNPFLALNCAAIPYTLFESEMFGTERGAYTDASSRSGIIEQANNGTL
                     FLDEIGSLSPVSQPCLLRVLDNGEFRRLGSLHSRSSKFRLVTASSRNPIELASQHEFR
                     DDLLFRIANFVIETPPLRERKEDIPLLAQHFCDQYSIGRFNIGSDALDKMYSHNWPGN
                     VRELFSVIMSACSLRKSGKICAKDIHFLPGIEKMSDQRKRNSR"
     gene            complement(3055..3762)
                     /locus_tag="PLQ30_05550"
     CDS             complement(3055..3762)
                     /locus_tag="PLQ30_05550"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HPW01945.1"
                     /translation="MESVSIACAFLDGLRLDFPSQFDLFILPYLWDDDKNHQICFVDK
                     QNWMHARKRDEFGSMIASADLILPRDSGIAENVLAHGEKALRCWTVPFIYQVLLDVLN
                     ESMVEENTVGLENMKEQIRAYKPHKVLTLLLSSLEKRNGSVFLLGGNPITLIKAEKNI
                     RATYPGITIVGSMHGLYRPEEEPAIIEAMQKGAPQMILAGSPLPAEECWIPRHMNVTR
                     SGIFFYHKPIMKWISGR"
     gene            complement(3750..4574)
                     /locus_tag="PLQ30_05555"
     CDS             complement(3750..4574)
                     /locus_tag="PLQ30_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HPW01946.1"
                     /translation="MRQIVLLGNQRGRESCSLDEKTSHYLINVRRMRKGDSFEAVDEA
                     GSRFSCTIVSDDSHAVKLEIKPIRFSRSLVGTPLNKAEEDKAKPSVAPTLHIALVQAL
                     PKGHKFDLIIRQAVELEVELIVPLITQHCVEQEPPERAKAKLERRKRIIREAIQQSGS
                     FVYTEILSAADLETLDAALNAKGFNKKDSVRILFHESEKEESQALHLLLSGDKDRVVL
                     AIGPEGGLSQNDCAAFIDLGFALHHARGPVLRVETAAAFAISAVRAIVFERGLWKV"
     gene            complement(4590..6059)
                     /locus_tag="PLQ30_05560"
     CDS             complement(4590..6059)
                     /locus_tag="PLQ30_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPW01947.1"
                     /translation="MKHIDMPAIRKQIIPFISLVLVLALSFLSVSIPELYAQQLKIPD
                     SKQYSLLLQNIYQFILQNYVEEPDPTKLYEGAVKGMLDSLNDPYSTYLDEDMMSDLMS
                     DTTGIYGGIGLYISKQATVANEDAPRYIEVVSPIEDTPAWREGIRPGDLIIKIDGEDT
                     SPMSTDKASAKIRGEAGTTVTLTFRRGTYEFEITFIRTKIEIPALKSAVIQTVNGNIG
                     YIRIIEWNPNTAPQMKTVLQDMKKQAIDKFILDVRSNPGGLLTSVVEVSDLFLSTNVI
                     VSTKGRSYSENYEYKAKPDLVIPLTDRMIVLIDQGSASASEIFAGAMKDTKRALLVGE
                     KTYGKGSVQQIFPLDKGGFKLTMAHYYTPSGIDIDKVGIEPDVKVPEPTLNEKELEEL
                     QRLYDAGEIARFLESNPKPTLTERKAFAAELAQKYKIQEIFLEKIIRDEAERSMPARI
                     FDLEYDIQLQKALDLIQSADFEQILESTKTLAQGRSTSN"
     gene            6118..6675
                     /locus_tag="PLQ30_05565"
     CDS             6118..6675
                     /locus_tag="PLQ30_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01948.1"
                     /translation="MSHNFATVLTIEDRPTRLLFDMRRSLFLLIASILAFLRVFVIGV
                     VAILFGMSDYGGVTTALLRVFQLYNLLFVYIVIIQYVKAGAREYLRIPSLIVACVSPI
                     LSALALMAYIRNIGGSFPVDMAKAAISMVALILLDIVVFGLLVIDALKFQLPKAKNAE
                     NLENMENMEKTENMEKTHKDQHEVS"
     gene            6675..7799
                     /gene="lgt"
                     /locus_tag="PLQ30_05570"
     CDS             6675..7799
                     /gene="lgt"
                     /locus_tag="PLQ30_05570"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPW01949.1"
                     /translation="MVAMLEFPKWLSPEIIPGLPFRWYGLMYLIAFGVTWLLFKRESR
                     RLGAPWTEDEAANFFIWGIVGILVGGRLAGTLIYEPTNYYWIKPWYIFWPFDESGAFV
                     GFQGMSYHGGFIGLIVASVIWCKIHKWNWFDWADLIAVSVPLGYTFGRLGNFINGELW
                     GKVSTVPWAMVFPNAERFSAKEQWVQEIARKVGIRIVSMNDMVNLPRHPSQLYEALLE
                     GIVLWLILWFFVRPRRSFRGEGVAVYSLGYGIARFFVEYFREPDSGLGYILALGDPDA
                     PTALFASPFNFSMGQILSFIMIVGALIFLFYAKKHFKIKPGEPIFIDASRVGKVDNSA
                     RVKSQGNTDGTSGGNNARSTKYDASSARKLRKKIKSGGER"
     gene            7796..9517
                     /locus_tag="PLQ30_05575"
     CDS             7796..9517
                     /locus_tag="PLQ30_05575"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPW01950.1"
                     /translation="MKISLIQLNSTIGDFKGNTDRVVAMVDRVLSLFPALEKADLVVF
                     PELCLCGYPPMDLLDQESFCLGSLAALRRLQKLLPSDLAVGVGYVDRNRNRTGKSLVN
                     AYSVIQGGKVVFTQIKTLLPTYDVFDEARYFEPAKERNLFIQPWGKVGFAICEDFWWE
                     APDASQKYAIDPVKDLMDEGADIIVVTSASPFVAGKIRTRLNLAEKAVRAGSIPVLYC
                     NAVGANDSLVFDGRSFAMGEDASIRGICGWAEEALSYDSVSGKAKRVLFDPLLQKAEF
                     AQENQLPGSDSFEEIRRAIVVGIQDYLKKSGFSKVVLGLSGGIDSALVAVLAAQAIGR
                     QNVTCIAMPSRFSSEASLDDAVELCRRNKLRLERIPIEAPFTSYLDALSVPFAGKPYD
                     TTEENLQARIRGTLLMAWSNKFNALLLTAGNKSELATGYCTLYGDMNGSLAPIADLYK
                     TQVYGLTGYLNRMAAENGQTEPIPESIIAKAPSAELRYNQKDQDTLPEYKVLDQILQL
                     YIEENLSMEEIVNLGFDRAIVRKTLEMTGKAEYKRRQAAPAIKLSKRAFGVGRRLPLA
                     RALHEIE"
     gene            complement(9444..10292)
                     /locus_tag="PLQ30_05580"
     CDS             complement(9444..10292)
                     /locus_tag="PLQ30_05580"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPW01951.1"
                     /translation="MKIRHLPVLPKFPRPLLFAHRGLNRKYLENSMSSFEAAFHAGVP
                     GIELDVHLTADEKLVVFHDDTTGRIELKIQPEEPARNLGLEVSTLIELRSLSIGTHIP
                     LLDEVFEYFGDSLYYDIELKSRSKADTGLASKVAAAICSHNLESNCIISSFNPFALCH
                     FRKAKSTIPVGIIWSNSNELYWFLRHGEGALISKSDFLKPESSLFANMASRSPLHLLP
                     WFSGSQEKPIIPWTVNEPEKACDFIDQGVAGIISDIADEILIRFRAEREPAAAFDPPQ
                     KLSLKA"
     gene            complement(10345..10944)
                     /locus_tag="PLQ30_05585"
     CDS             complement(10345..10944)
                     /locus_tag="PLQ30_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01952.1"
                     /translation="MTPQELSKLDYEGASQMILAYTIDAKKYEKEIAALRSEAQDWKA
                     RAAEAQSKAVPELSQAALQRASQLESKALELSAELVAIRRDIEQLQEALPIIKAKQRR
                     VDPDQLLAELSMIVGLDNLGEQQGSSRRGDSTTSNADKDSKTIKDQDELNNKGSTSSA
                     DTTNRASLEKPINPPDEQSPVDHALDDALAELKKKMGLL"
     gene            complement(11008..>11411)
                     /locus_tag="PLQ30_05590"
     CDS             complement(11008..>11411)
                     /locus_tag="PLQ30_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW01953.1"
                     /translation="VLQPGDHGTTFGGGPLAASAALVVLSELKRAGFLDEVDRKGRRL
                     RALIESFHHPIVKDVRGMGLMIGVGVKVDPHEVVSAAQSHGLLLLTAGDDTVRLLPPL
                     VVTDKDIEEGAAALELALSDVYTATNLVHMV"
BASE COUNT         2872 a         2635 c         2677 g         3127 t
ORIGIN      
        1 aagctcctga tattcgattc cgaggtcgat gagatagctt tctattctgg tcaatgctga
       61 cattactacc tcctccttgt acttctattg tcttagatct atgcgctcaa ccgagcgtaa
      121 tagtcccatc cgggaatgcc gtgatgacag cttcacatgt ctgacatcgc cccctgccgg
      181 gcttttctat atctataagg gagttgcagg cagggcactg gacagagagg ggatagatgc
      241 catttagggc gaatttgaga cctgtcagcg cgcttttggc atccgttgca gttgtaaaat
      301 agccctccag tccaagaatt cgctccattc gttccatgcg aggccgcata ccacagagca
      361 caagatcgcc ctgccggtct ctcaattttt gcctgagggc aagcatgagc cccatacctg
      421 ctggccctat atcttcgata gtggaaaaat cgaccaccag atagcgtatg ccttcggaga
      481 ggatactatc gaacgattgg gcaactgcat ccaggttggc ttctgtaacc gcgcccacga
      541 agcgcgccaa aatgcatcct gggaaatccg tagatacctg ggacgagatg agagcatctg
      601 aaccttgctg agaaatcttc tgaattcgcg aaagaagccc cgatggcata gtgtatcaat
      661 aatatgggtt agggtagaaa aagtcaaaca cccagaaaag tcaaataccc gcttacgcaa
      721 aacccttatg tcacaagact cttatggtgc aaaaccgccg tccaggttga ccacagctcc
      781 ggatgcgata cagggttcgg aatcgataag atcgagagct attttcgcta ttatattggc
      841 ttttaagagc gaaccggtag ctgtcagcga tgcatggcgt atcttggttt ctctatcaag
      901 atattcggtc tctacgggac cagggcacac tacgatagcc gcgacattgt cggaagcgct
      961 ttgcacggca atggactttg ccagcacacc aagcccagtc tttgaagaag cataagtagc
     1021 ggtcttcttg taggagcgta tgccatctgt ccttgtacca ccaaaaaaca gaaagcgacc
     1081 gtactttctt tccatcatgc caggcaagaa tcgcgatgct aaggctcctg gcagggcaag
     1141 attggctagt gtaagccatt cccaatctat tgcggtacaa tcttttaagg gtttttccag
     1201 gaatggacca aaggcgcaga caactatatc gggttccttt cctatctcat ctaggcgcaa
     1261 gtctatggcc tgaatgaact ggacgggact tgaaaaatcc aaatctatca agttcaggct
     1321 ctttgctttt ggaaagctgt ctactcgcaa tttttccgaa gcacggcgtc cctgtattag
     1381 cagggctgca cctctgctgg caagttcata ggacacggca agaccaatac cgcctgagcc
     1441 gcctatgact aaggcgagtc tttcgctgaa tgtcgatacg cgattctgta attgaatgct
     1501 gccttggata tcttctgaat tattattatt catgtcttga gaatactgga atttctcctt
     1561 attagatagc gacattagat agcgacatta gatagcgacn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnna ctggaatttc tccttattag atagcgacat tagatagcga
     1741 cattagatag cgaccgctgg cggcaattct ggccaaagct cttatttaac ggctatttcg
     1801 cttgcgctgg tctgacatct tttctatgcc cggcaaaaaa tgtatatcct tagcgcaaat
     1861 cttgcccgac tttctgagcg agcacgcact cataattacc gagaatagct ctcgaacatt
     1921 gccaggccaa ttgtgggaat acatcttgtc caaggcatca ctccctatat tgaagcgacc
     1981 aattgaatac tgatcacaga aatgctgcgc aagcaaagga atgtcttctt ttctctctcg
     2041 cagaggagga gtctctatca cgaaattggc aatgcggaat agcagatcgt ctcgaaactc
     2101 atgctgagag gccagctcaa ttggatttcg gctcgaggct gtaacaagcc gaaatttaga
     2161 cgagcgagaa tgaaggcttc cgagtctacg gaattctcca ttatcgagta cgcgcagcaa
     2221 acatggctgc gaaacaggag aaaggctacc aatttcatcc agaaagagcg taccattatt
     2281 tgcctgctcg atgatgccgc ttcttgagga agcatcggta taggctcctc tctcagtgcc
     2341 aaacatctca ctttcaaaaa gagtgtatgg aattgccgca caattcaatg ccaaaaaagg
     2401 attcgatctt ctgtcgctcc attcgtgcag agcgcgcgcg gctagttctt ttccggttcc
     2461 tgtttctcca atgatcataa cagaacaaga ttcgttggct aacatgcgaa tctgcgaaat
     2521 caaatgacct atttttttgc tttttccaat tatatttaaa gaagaatcag catgtcgagg
     2581 ttttgcacca gatgccttgt cgccacagga aggcaagtgt tccttatctg atttttccga
     2641 gacgctggca actatgtcat tgagttctaa ataatcatgg aaataatatg tctctaatga
     2701 agagggaacg attttcttca tgatttcttt ttggcgcgca ttgaagtctg aatctgatga
     2761 tataagtcct acaataagaa tttccggctt ctgcctttgt atcattatgg ataattcgag
     2821 caagtctccc gcgacagaaa tatcaaaata cttgctaaag agtcttatta taggccgtat
     2881 tcttctaaag tcctgatcaa aatagattag tcttttcatg attgcttgag atgtaacaaa
     2941 tgcatctcta tgtttcaagg cctaaagaac aactttgtcg aagtaagata tttttaccta
     3001 atatatttca aatattagct aacatctaat tatgtctaca gagggcaaaa agttttatct
     3061 accggaaatc catttcatta tcggtttatg ataaaagaaa ataccgcttc gggttacatt
     3121 catgtggcgg ggtatccaac attcttcagc agggagggga ctacccgcta gaatcatttg
     3181 aggagcccct ttttgcatag cctcgatgat agcaggttct tcctctggcc gatataagcc
     3241 atgcatgctt cctactattg tgattccggg ataggtcgca cggatgtttt tttcggcctt
     3301 gataagcgtt atcgggtttc cacccaataa gaacacggag ccattgcgct tttcgagaga
     3361 agatagtagg agggtaagaa ccttatgggg cttataagct ctaatctgtt ctttcatatt
     3421 ctctaatccg acagtgtttt cttctaccat ggattcattg agaacatcca ataatacctg
     3481 gtaaataaaa ggtacagtcc aacagcgaag agctttttcg ccatgcgcga gcacattctc
     3541 cgcaatgccg ctgtcgcgag gtaaaatcaa gtcagcactg gcgatcatgg atccaaattc
     3601 gtcgcgcttt ctagcgtgca tccaattctg tttatcgaca aaacaaatct ggtgattttt
     3661 gtcgtcgtcc caaagatagg gcaatataaa cagatcgaat tgagaaggaa aatcgagacg
     3721 taagccatct agaaacgcgc aagcgatgct tacactttcc ataaacctct ctcgaaaacg
     3781 atagctcgaa ctgccgatat tgcaaaagca gcagcggttt ccactctcaa tactgggcct
     3841 ctggcatgat gcagagcaaa tcccaaatca ataaatgccg cgcagtcatt ttgagaaagg
     3901 ccaccctctg gtcctatggc tagaacaacg cgatccttgt cacccgaaag gagaagatgc
     3961 aaggcttggg attcttcttt ctccgattca tggaatagaa ttcttaccga gtctttttta
     4021 ttaaaaccct ttgcgttcaa ggccgcatct agcgtttcca gatctgcagc cgacagaatc
     4081 tctgtgtaga caaaagagcc cgactgttga atagcctcgc gaattatgcg ctttcgccgt
     4141 tcgagctttg cctttgctcg ctctggtggc tcctgttcaa cgcagtgctg agtgatcaat
     4201 ggaacaatga gttctacctc cagttctacc gcctggcgaa ttatgagatc gaatttatgg
     4261 cctttgggca gcgcctggac cagggctata tgcagagttg gagctacgct tggttttgct
     4321 ttatcttctt ctgctttatt caagggggtg cccaccagag atcgggaaaa gcgaataggt
     4381 ttaatttcaa gctttactgc gtgagaatcg tcggaaacaa tggtacagga aaaacgcgaa
     4441 cccgcctcat ccactgcctc aaaagagtcg ccttttcgca ttcgccgaac atttatcagg
     4501 taatgactcg ttttttcatc gagcgagcaa gattctcggc cgcgctggtt tcccaacaga
     4561 acaatctgtc gcataatgca aaaccttatc taattgcttg tggagcggcc ttgggccagg
     4621 gtcttggtgc tctctaagat ctgttcgaaa tctgccgatt ggatgaggtc gagagccttt
     4681 tgcagctgta tatcatattc gaggtcaaag attcgggctg gcatagacct ctcagcttca
     4741 tcgcgaatta ttttttcgag aaaaatctcc tggattttgt atttctgtgc aagttccgct
     4801 gcaaaagcct tacgttctgt aagagttggc tttgggttgc tctcgagaaa tcgcgctatc
     4861 tcgcctgcat cgtagagacg ttgaagttcc tcgagttctt tttcgttaag ggtgggttca
     4921 ggtaccttta cgtccggctc gattcctacc ttgtcgatgt cgatgccact tggtgtatag
     4981 taatgtgcca ttgtcagctt aaatccccct ttatccagag ggaaaatttg ctggacagat
     5041 ccttttccat aggtcttttc tccaaccagg agagccctct ttgtatcctt catggcaccg
     5101 gcaaagatct ccgaggctga agctgaaccc tgatctataa ggacgatcat ccggtccgtt
     5161 agaggaatta ccaggtcagg ttttgccttg tactcatagt tctcagagta gctgcgccct
     5221 tttgttgaaa cgattacatt ggtagaaaga aaaagatcgc tgacttcaac tacggaagtc
     5281 aggagccccc ctggattcga ccgcacgtct aaaatgaatt tatcgatggc ttgcttcttc
     5341 atatcttgga gcaccgtctt catctgcggc gccgtgttcg gattccactc gattatgcga
     5401 atatagccga tatttccatt taccgtctgg atgaccgcgc tcttgagtgc agggatttca
     5461 atcttagtcc gaataaatgt aatttcgaac tcataagttc ctcttctaaa cgtgagagtg
     5521 acggttgtgc ccgcctctcc tcggatcttt gctgaggcct tgtcggtgct cataggagag
     5581 gtgtcttcgc catctatctt gattatcaga tcaccagggc gaattccttc tctccatgca
     5641 ggcgtatcct ctatcggaga tacgacttcg atatatcttg gtgcgtcctc gtttgccacg
     5701 gttgcttgct tggagatata gagacctatc cccccataga taccggtagt gtccgacatc
     5761 agatcagaca tcatgtcctc atctagatat gtggaatagg gatcgtttag tgagtcgagc
     5821 atccccttta ccgccccctc gtagagcttg gtcgggtctg gttcctcgac atagttctga
     5881 agaataaact ggtagatatt ctgcagaaga agggaatact gctttgaatc aggaatcttt
     5941 agctgctgcg catagagctc cggaatcgaa acgctaagga acgatagagc tagcacaagc
     6001 actaggctaa taaagggaat tatctgcttt cttattgctg gcatatctat atgtttcatc
     6061 gggttggact ccttttcccc gagagtagtg ttttcatgag tcacattatc atagccaatg
     6121 agccataatt ttgctactgt cttgactata gaagataggc ctactagact ccttttcgat
     6181 atgcgacgtt cgcttttttt actaattgcc tcaattttgg cttttctgcg tgtctttgta
     6241 attggcgttg tggcaattct atttggaatg agcgattatg gtggtgtaac aaccgcgttg
     6301 cttcgagttt tccaattgta taatctctta ttcgtctaca ttgtgataat tcagtatgta
     6361 aaagcgggag cgcgagaata ccttagaata ccctcgctca tcgtggcctg tgtttctccg
     6421 attctcagtg cgctagcctt gatggcctac atccgcaata ttggcgggtc ctttcctgtg
     6481 gacatggcaa aggcggctat ctccatggtc gccttgattt tgcttgatat tgttgttttt
     6541 ggcttgcttg tgatagatgc gcttaagttt caactgccca aggccaaaaa tgcagaaaat
     6601 ctggaaaata tggaaaatat ggaaaagaca gagaatatgg aaaagactca taaagatcaa
     6661 catgaggtct cctgatggtt gcaatgctcg aattcccaaa gtggctttct ccagagataa
     6721 ttcccggtct gccctttaga tggtatggcc tcatgtatct aatcgccttt ggcgtgacct
     6781 ggcttctctt caagcgagaa tccagacggc tcggtgctcc ttggaccgaa gatgaagcgg
     6841 cgaatttctt tatctgggga atagtcggaa ttctagttgg gggtagacta gctggcacac
     6901 tgatatatga gccaacgaac tactattgga tcaaaccatg gtatatcttc tggcccttcg
     6961 atgaatcagg ggcatttgta ggtttccagg gtatgtctta ccatggcggc tttatagggc
     7021 taattgtcgc cagcgtgatc tggtgcaaga tccataaatg gaattggttc gattgggcgg
     7081 acttgatagc ggtttctgta cctcttggct atacctttgg caggcttggt aatttcataa
     7141 atggtgagct ctggggaaaa gtcagcaccg taccctgggc tatggttttt ccgaatgcgg
     7201 agcgattttc agccaaagag caatgggttc aggaaatagc gcgcaaagtg ggaattcgaa
     7261 ttgtctccat gaatgacatg gtaaatctgc ctcgtcatcc ttctcaactt tacgaagctc
     7321 tgctcgaggg cattgtgtta tggctcatac tatggttttt tgtgcgtcct cgccgcagtt
     7381 ttcgcggcga aggtgttgcc gtatattcgc ttggctatgg aatagcacgc ttctttgtag
     7441 agtacttccg agagcctgat tcgggtttgg gttatatctt agccctagga gatccagatg
     7501 cgcccacggc gctctttgct tctcctttca atttttcgat gggccagatt ctaagtttta
     7561 ttatgatcgt tggggcgcta atattcctct tttatgcaaa aaagcatttt aagataaagc
     7621 cgggagagcc catatttata gatgcatcga gagtcggaaa agtagataat tcggccagag
     7681 taaagagtca agggaacact gacggcacga gtggcggcaa caatgccaga tccacaaaat
     7741 atgatgcctc tagcgcccgc aagctgcgca agaagatcaa aagcggagga gagcgatgaa
     7801 aatctcgcta attcagctca attccacaat aggagacttc aaaggcaata ccgaccgagt
     7861 ggtagccatg gtcgatcggg tgctttcctt gtttcccgct ttagaaaagg ctgatcttgt
     7921 agtcttccct gaactatgtc tttgtgggta ccctcccatg gatcttctag atcaggaaag
     7981 tttttgcctg ggttctttag ccgctcttag aaggctacaa aagctattgc cttcggattt
     8041 ggcggttggc gtgggctatg tcgacagaaa tcgcaataga acagggaaat cgctagtcaa
     8101 tgcatattcg gtcatccaag gcggaaaagt tgtttttact cagatcaaaa ccctccttcc
     8161 tacttatgat gttttcgatg aggccaggta cttcgagcct gccaaggaac ggaatctttt
     8221 tatccaacca tggggtaagg tcggctttgc gatctgtgag gatttttggt gggaagcgcc
     8281 tgatgcctcg caaaaatatg cgatagatcc ggtaaaggat cttatggacg aaggggccga
     8341 cataattgtc gttacttcgg cttcgccttt tgttgctgga aaaatcagaa caaggctcaa
     8401 cctggcggaa aaagctgtac gcgcagggtc tattccagtg ctctactgca acgcggttgg
     8461 agcaaacgac tcattggtct tcgatgggcg ttcttttgcc atgggagaag atgcttccat
     8521 tcgcggaata tgtggatggg ctgaggaagc actgagctac gacagtgtta gcggcaaggc
     8581 caaacgtgtg ctcttcgacc ctttgcttca gaaggcagag tttgcccaag agaatcaact
     8641 tcctggctca gatagtttcg aggaaatccg gcgagcgatt gtcgttggaa ttcaggatta
     8701 tcttaaaaaa tcaggatttt ctaaggtagt cctgggcctt tccggaggca tagattcagc
     8761 actggtagct gtcttggctg cacaagccat aggtcggcag aatgtcacct gcattgcaat
     8821 gccaagccga ttctcgagcg aggcatctct agacgatgct gtcgagctat gtaggcgcaa
     8881 caagctaaga ctcgagcgaa taccgataga ggccccattt acatcatatc ttgatgcgct
     8941 ttcagtacct tttgccggca agccttatga caccaccgaa gagaatctgc aagctcgcat
     9001 tcgaggaact ctgctgatgg cttggtcgaa taaattcaat gctcttctgc tcaccgcagg
     9061 caacaagagt gagcttgcca caggctactg caccctctac ggcgacatga acggctccct
     9121 tgctcctatc gcggacctct ataagaccca ggtctatggg cttactggct atctcaatcg
     9181 aatggctgct gaaaatggcc aaacagagcc catccctgag tccatcattg ccaaagctcc
     9241 gagcgccgaa ctgcgctata atcagaagga tcaggatact cttccagaat acaaagtgct
     9301 agatcaaatc ttacaactat atattgaaga aaatctctcg atggaagaaa tcgtaaactt
     9361 aggattcgat cgagctatag tacgaaaaac gttggaaatg accggcaaag cggaatacaa
     9421 gcgcaggcag gcggcacctg cgatcaagct ttcaaagaga gcttttgggg tgggtcgaag
     9481 gctgccgctg gctcgcgctc tgcacgaaat cgaataagta tttcatctgc gatgtccgat
     9541 ataattcctg ccacaccttg gtcaatgaaa tcacaggctt tttccggctc atttaccgtc
     9601 catggaatta tgggcttttc ctgcgagccg ctgaaccatg gaagcagatg cagcggcgat
     9661 cggctggcca tatttgcaaa caagctcgat tcgggtttta gaaaatccga tttcgaaatg
     9721 agcgcgcctt ccccatgcct tagaaaccaa tagagttcgt ttgaattgct ccagattatg
     9781 cctactggta tagttgattt cgcttttcta aagtgacaca gagcaaatgg gttgaaggaa
     9841 gagatgatgc aattgctctc gagattgtgc gaacagatcg ctgctgcaac cttcgaagca
     9901 agcccagtat ctgccttact gcgagatttc aactcgatat cgtaatatag agaatcacca
     9961 aagtactcaa aaacctcatc gagaaggggt atatgagtgc ctatggagag cgatctgagc
    10021 tcgattaggg tggaaacctc aagccctagg ttacgagcgg gctcttcggg ctgaattttt
    10081 agctctatac ggccggtagt gtcatcgtgg aaaacaacca gcttttcgtc ggctgtaaga
    10141 tggacatcga gctctatgcc aggcactccg gcatgaaagg cagcctcgaa agagctcatt
    10201 gagttttcca ggtattttcg gttgagccct cgatgggcaa aaagaagagg tcgcggaaat
    10261 ttaggcaaca ctggcaaatg gcgtattttc atcctgctat tacctataat tgataattga
    10321 tcaaagagat gacataagac aaagtcagag aagacccatt tttttcttaa gctcagcgag
    10381 agcatcatcg agagcatggt caacagggct ctgctcatct ggagggttta tgggtttttc
    10441 gaggctagcg cggttcgtgg tatcagcaga actggtggaa cctttgttat tcaattcatc
    10501 ttgatccttt attgttttgg aatccttgtc ggcgttcgat gttgtcgaat cgcccctgcg
    10561 actagaacct tgctgctctc caagattatc cagaccgact atcatcgaga gctccgcaag
    10621 gagttggtca ggatcgactc tgcgttgctt tgccttaata attggaagag cttcttgcaa
    10681 ctgttcaatg tcgcgacgaa tagcgacaag ctctgccgaa agctccagag ccttcgattc
    10741 cagctgcgat gccctctgaa gcgcggcctg agaaagctca ggaacagctt tactctgcgc
    10801 ttctgcagcc ctggctttcc aatcttgggc ttccgatcta agcgcggcaa tttccttttc
    10861 gtatttcttt gcatcgattg tataggcgag gatcatctgc gaagcacctt cataatcgag
    10921 cttggatagt tcctgcggag tcattgggtt ttacctcgcc tctattgtac ctgaatcatc
    10981 gctttacagt aagcattcct tactgcttca aaccatatgc acaaggttcg tagcagtata
    11041 gacatcactc agcgcgagct ctagcgccgc ggcgccttct tctatatctt tgtctgtcac
    11101 caccaggggc ggcaagagcc gcacggtatc atcaccggcg gttagaagaa gaagcccgtg
    11161 agactgtgcc gcgcttacaa cttcatgtgg atcgactttt acgcccaccc caatcataag
    11221 gcccataccg cgcacatctt tgacaattgg atgatggaaa ctctctatga gtgctcttag
    11281 gcggcggccc ttgcggtcga cttcgtccaa aaaacccgct cttttaagct ccgatagcac
    11341 aacaagagct gcggaggccg caagtgggcc gccgccgaag gttgtgccat gatcgccagg
    11401 ctgcaaaact t
//
LOCUS       DAOMZL010000054        11218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_5429_length_11218_cov_15.373376, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000054 DAOMZL010000000
VERSION     DAOMZL010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 11218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11218
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5429_length_11218_cov_15.373376"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1442
                     /locus_tag="PLQ30_05595"
     CDS             162..1442
                     /locus_tag="PLQ30_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW01954.1"
                     /translation="MKRKTLILLLAIVSAGVALAAPASQKTINFLGVWGGQEAEVFNA
                     MAKQFEDKTGIKVELEATRDLDAVITTRVEAGNPPDIAGLPGPGKLIELAQAGKLVEL
                     SKILDMKEFDKNYSSGWKSLGTVDGKLYGVFMKAATKGLVWYNPKTFKDKDLPVPTNE
                     WTWDQMMALSKQAIAKGISPWSIGIESGAASGWVGTDWLENIFLRVNGPDKYRDWYEG
                     KLAWTSPEVKKVWEIWGQIVADPKMAYGGSRYINSTNFGNAASPLFTKPPQALLHQQA
                     SFIQGFIMDQFPTLKPVEDFDFVAFPAIDPRYSKAVEAAADVIAVFRDTPEVKAFVNY
                     LTSAEAQAFWAAGTGALATNKNVSLVFYSDPLIRRAADILNKSEIVVFDASDMMKSEM
                     NAAFWSAVVSYVENPKNLDSILAQLEKVRKEVYR"
     gene            1536..2522
                     /locus_tag="PLQ30_05600"
     CDS             1536..2522
                     /locus_tag="PLQ30_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPW01955.1"
                     /translation="MGVHTNYILLAIIALAVIPIFLWVYLLISEWIASHTNRRAASIL
                     RPWFWILPAISLLVVFLVYPVINTIILSFMDSRSENFVGLKNYIYIFTDPNMLIVLRN
                     NLLWLVFFTAACLVLGLVIAVLSDRVRYESVAKAAIFLPMAISFVAAGVIWRFMYEYR
                     PAGSTQIGTVNAILTTLLPNFEPRAWLFDRSTNNAALIVVGIWMWTGFAMVILSAGLK
                     GISVDVLEAARIDGAGEVAIFFKIILPLMMPTITVVATTLIINVLKVFDIVYVMTNGN
                     LGTEVIANRMYKEMFNYRNYGRASAIATLLLFAIIPVMIMNIKRFNAEEGNE"
     gene            2515..3387
                     /locus_tag="PLQ30_05605"
     CDS             2515..3387
                     /locus_tag="PLQ30_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPW01956.1"
                     /translation="MNKKGKISGNSSKKPLSNLLVHLILIVICVAWIIPSLGLFVSSF
                     RPRSEIALSGWWQAFQPPFAFTLENYSYVLTRGNMGRSFVNSLLIAIPSTLMPIFIAA
                     CAAYALAWMRFPLRKTIHLLIVALIVVPLQMTMIPILRLFNKIGLTGSFFGIWLAHTA
                     YGLPFAIYLLRNFIVQLPRSLIESAQIDGASHPIIFLRLVLPTSIPAISSLAIFQFMW
                     VWNDLLVALIYLGGTPNVAPMTVTISNMVNSYGGGWEYLTAAAFISMLLPLIVFFALQ
                     KYFVRGILAGSVKG"
     gene            3399..4430
                     /locus_tag="PLQ30_05610"
     CDS             3399..4430
                     /locus_tag="PLQ30_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPW01957.1"
                     /translation="MKKQSVSASDVAKMAGVSRTTVSFILNNTQGKHISEATRQRVLK
                     AAEALQYVPDMNAISLAKKAHYSIGFFICYSRAIFSDAYLFRLIEGITLVLNKHHCRL
                     ELHSLRMTRRNYLDLVHSSKLDGLILMNTHTDDPGIKELEEAKIPFVVIGSIEGLSVP
                     QVDIDNVAAAREMVDYLVSLGHRKIAMIAHAPLEFYAAANRLEAYRQVLNENGIPIIS
                     KYIEVADFSEESGYRAMQRLLCLEDRPSAVFAGNDMVAYGAMQAIIDEGLSIPEDISI
                     AGFDDDFLSRYLNPSLTTVAQPSEGLGQAAAKLLIQEIISPRKSGLRERDYIVLPTMV
                     AKRESCRRI"
     gene            4434..7544
                     /gene="pgmB"
                     /locus_tag="PLQ30_05615"
     CDS             4434..7544
                     /gene="pgmB"
                     /locus_tag="PLQ30_05615"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HPW01958.1"
                     /translation="MSSHDAWKITEPGFSIDHIDTYETLFALANGNLGLRGDFDEKYP
                     AFHKGTYINGFFEKEPITYGEIAYGYAENHETILNIPNAKYIALFVDGEEFRLDAIEP
                     SRIEDYQRYLDIRNGVLVRIVRVRLSSNVLIEIRSTRLVSFLRESIAAIKYEVCLLET
                     PNRWPFVVRLESCIEGRVFNRRAEGDPRVGSKFQRDAYAIDRLEYDNSEARILITTQL
                     SRLAATAQIRHVFSGPRSMQVETAIEGSTVIHGWNGTVEAGQKFSLVKYMAYQMAALE
                     DAGSCWAEAHQELIRASQDGFEVLFNEQRSFLSEFWERADVVVEGDSKVQQALRFNLF
                     HVLQSTGRKGKTGLAAKGLTGEGYEGHYFWDTEIYVLPFLIYTMPEIARKLLEYRYSI
                     LDKARQRAKIMSEKGALFPWRTIDGEEVSAYYPAGTAQYHINADIIYAMEKYCKASND
                     IEFLYGPVAEVAAETARLWISLGHYSEDKRFRIDEVTGPDEYTVLVDNNCYTNLMAKN
                     NLAFAARVLKEMQENRSQAFEALRKKIGLLDSEIGFWESAAEAMYIPYDKKTGIYAQD
                     DSFFEKERWPFDETPADRYPLLLHYHPLVIYRHQVLKQPDLVLAQFLLSEKFSLAEKK
                     RNFKFYEPLTTGDSSLSHCIQSIMASETGDAKAALDYFMKTVRMDLDDVHGNSRDGIH
                     TAAMGGSWLSVVYGFAGFRDRASAGDEALFDSQAAAVDQAPSADRAVADVKAYYSFNP
                     RLPEGWQRLSFSLQLGSQRLDIDIKKDRVRYARETEFQARVQPSSEASREALAFKHKK
                     ELIVLKPGESVEISLKPVFRAAIFDLDGVVTDTARYHFQAWKKLGEEHGWQVDEALNE
                     KLKGVGRMESLDIILKENAIDLPASQKIELAERKNSYYKEFLTRLTPEDILPGMKSLL
                     LELGNHGIKRVLASASRNAPTIIERLGLGPIPGQFFDALADPNAVTCGKPDPELFLLA
                     ADLAGASPEDCVGIEDAQAGIIAIKAAGMKALGVGEGLTGADIVVESTSEISFQMLES
                     LFRSKS"
     gene            complement(7635..9092)
                     /locus_tag="PLQ30_05620"
     CDS             complement(7635..9092)
                     /locus_tag="PLQ30_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPW01959.1"
                     /translation="MHFKSILRMLSIILLIVSIFSLICGIYSIVEDLSWRVSFSLFIP
                     TIISLAFFIVMYLMTRHDERPFLTNKDSFLFVAFSWVFSSALGAIPFTFSGYIPSYID
                     AFFETVSGFTTTGASILTDIEIIPRPLLLWRATTHWLGGMGIVVLLVAILPSLGFSAI
                     RIMEAESPSPEVDRITPRISKTAKTLWLIYCGFTVCEIILLVLGGMPLFDAICHSFAT
                     MATGGFGTKNANIAYYNSGFIQWIITIFMFLAGTNFTLHYHFLKGRMSTILLDTEFRV
                     YLGIFAISSVIIAVDLFVSGVFGNIGDSLRHASFQVSSILTTTGFASTDFGQWPQLSQ
                     TVLFFLMFIGGCSGSTGGGVKVIRLVVLFKMAVNEMRYVTNPRGVYTIFLGNRALRKN
                     VIYDISGLVFLYFAFFFLSVLVIAFSGVDLLTNITAVIANLGNIGPGLGKVGPAFNYA
                     LFPWWAKLWLSLDMLVGRLEVYTVLALFTRKFWRA"
     gene            complement(9094..10665)
                     /locus_tag="PLQ30_05625"
     CDS             complement(9094..10665)
                     /locus_tag="PLQ30_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPW01960.1"
                     /translation="MQNANEAQYVDVDAPIYRAFSIFGAAQLLGIQQALHPSPVIVKI
                     ALGRIYFSSMKIAILGAGLLGSLIASELIAENKDVVIIEKNLNAAKSIADKLDCIVQE
                     GDGENIETLKAAGVEDADWFIACTGSDEANIVSCGLVAENFKDVRTIARARNPYFASF
                     KNAGKKILGVDYIINPEAETAESIAGTIFRGMSPEIIDVKEAGIQLRRILCKKEPRFV
                     HQSLANIRTSIGADFMVPAVLREEELIVPGGDFVFEEEDAAYILGEPSSLDRLFGPCK
                     DTLRKFSSIVILGTGTLTTLLLKELGMRQISAFSGSQRDKGYDRGALSLLGNPKIKVI
                     YGDRESLKILSATFPDIEAINHQLSDEHLIREENIGSADIVLCLASEQSINILSALLA
                     QEAGAKRSLALITNDLYTPLLYLLDINIVVNEKIVMSGAILDKVRKAKIRRLYSFPHN
                     EYELIEIQITDSFEQLEVEIRNMNLPKGLLICFVIHRGKTCIPTGSTKIFENDLVGLI
                     IKKEHIGKLESIFGA"
     gene            10690..>11218
                     /locus_tag="PLQ30_05630"
     CDS             10690..>11218
                     /locus_tag="PLQ30_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPW01961.1"
                     /translation="MRNRLGKEKQIPLLYFLGTILAGSFLLVLPISSEEGNLSYLDAL
                     FEATSAVCVTGLAVVDTTRLSRFGQTVILFLIQLGGLGIVTFSTLYIFMPRRTVSVTT
                     HGMIKDYTLSSVEYRTRRTIREIIAWTASFELIAAFVIWPTLAKNGYSFYDALFHAIS
                     AFCNAGFSTLRSGMES"
BASE COUNT         3037 a         2451 c         2685 g         3045 t
ORIGIN      
        1 accaccctct agtttcccta gccctatgcg tcaactcatt cctgacttgt acccaaggtc
       61 gcataagctc aatttcttaa attttccttg cgcgttttgg aaactaggct tataatgtta
      121 ctaaatagcg ttagtaacgc atagattcaa ggaggttctt catgaaaaga aagactttga
      181 ttctgttgct ggcaatagtt agcgcagggg tcgccctggc agcgccggca agccagaaga
      241 caatcaattt ccttggtgtt tggggtggtc aggaagccga agtattcaat gccatggcga
      301 aacagttcga agataagact ggcataaagg tcgagctaga agcaacacga gatctagatg
      361 cagttatcac aactagggtg gaggcaggta atcctcctga tatcgcgggt cttcctggtc
      421 ctggcaaact catcgagctc gctcaggccg gaaaacttgt cgagctatcc aaaattctgg
      481 atatgaagga attcgacaag aactattcct ctggatggaa aagcctgggg acggtggacg
      541 gcaagctcta tggcgtcttt atgaaggctg caaccaaagg ccttgtttgg tacaatccaa
      601 agacattcaa agataaggac cttcctgtac caaccaatga atggacctgg gatcagatga
      661 tggcgctctc gaagcaagcg attgcaaagg gcatctcgcc ttggtcgatt ggtatcgaaa
      721 gcggagctgc atcgggttgg gtcggcaccg actggctcga gaacattttc ttgcgcgtaa
      781 atggaccgga taaatatcgc gattggtatg aaggcaaatt ggcatggacc agtcctgaag
      841 taaagaaagt gtgggagatt tggggtcaga tcgttgcgga tccaaagatg gcgtatggtg
      901 gatctcgcta tatcaattcg acgaatttcg gaaatgccgc ttcaccgcta tttactaagc
      961 cacctcaagc gcttttgcac cagcaggcga gttttatcca gggattcatt atggatcagt
     1021 tcccgacgct aaagccggta gaagacttcg attttgtggc ttttccggcc atagatccga
     1081 ggtattcaaa ggcggtggaa gcagctgccg atgtaatcgc tgtgtttagg gatactcctg
     1141 aagtaaaagc ctttgtgaac tatttgacca gtgcagaggc acaagctttc tgggcagctg
     1201 gaacgggagc gctggccaca aataagaatg tttcactggt gttctattct gatccgctaa
     1261 tcagaagggc cgcagatatt ttgaacaaaa gcgagattgt cgtatttgat gcctcggaca
     1321 tgatgaagtc agagatgaac gctgcattct ggtctgctgt ggttagctat gtagaaaacc
     1381 ccaagaatct cgattcgatt ctggctcagc tcgaaaaagt acgtaaagaa gtgtatagat
     1441 aaatttcgtt ttgactggat gtaaaggaaa gcaggggaca aaggcttccc ctgcttttca
     1501 tctttctcga agcgtcggac caggtgagta gaggtatggg cgtgcacacc aactatattt
     1561 tattggccat tatcgcgctt gcggtaatac ccattttctt atgggtatat ttattgattt
     1621 ccgaatggat tgcttcacat acgaacaggc gagcagcttc tattctacgt ccatggttct
     1681 ggattcttcc agcaatatct ctcctagttg tattccttgt gtatcccgta ataaacacaa
     1741 tcatcttgag ttttatggat tcacgttccg aaaatttcgt gggtctaaag aactatatct
     1801 atatattcac tgatcctaat atgctcatag tactaagaaa caatttactc tggcttgtgt
     1861 tttttacggc agcatgtcta gtccttggtc ttgtcattgc agttctatcc gatagggtta
     1921 gatatgaatc agtagcaaaa gctgcgattt ttctccctat ggctatttct ttcgttgcag
     1981 caggcgtgat atggcggttt atgtatgaat atcgacccgc aggcagtact cagataggca
     2041 cggttaatgc catattgact accctattgc ctaattttga accaagggca tggctgttcg
     2101 atcgctctac aaacaatgcc gctctcattg tggttggtat ctggatgtgg acgggctttg
     2161 ctatggtaat tctctctgcg gggcttaagg ggataagcgt agatgtgctt gaggctgcaa
     2221 ggatagatgg agctggcgaa gttgcgattt tctttaagat aattcttcct cttatgatgc
     2281 ccacgataac agtggtggca acgactctaa taataaatgt attaaaggtc ttcgacatcg
     2341 tatatgtcat gacaaatggc aatcttggaa ccgaagtgat cgccaataga atgtataagg
     2401 aaatgttcaa ttatagaaac tatggaagag caagcgccat agcaacattg ctcctttttg
     2461 ctataattcc tgttatgata atgaatatta agcgcttcaa tgcagaggaa gggaatgaat
     2521 aaaaaaggaa agatttcagg gaattcatca aaaaagccgc ttagcaatct ccttgttcat
     2581 ctcattctca tagtaatatg tgtggcatgg ataattcctt cccttgggct ttttgtcagt
     2641 tcatttagac ctcgaagcga aattgccctt agcggatggt ggcaagcctt tcaaccccct
     2701 ttcgctttta ctcttgagaa ctattcttat gtgctgacac gaggaaatat gggcaggagt
     2761 tttgtgaata gtttgctgat agccattccc tcaaccctga tgcccatttt catcgccgct
     2821 tgtgctgctt atgctcttgc ttggatgcga tttcctttga gaaagaccat tcatctactg
     2881 attgtggcat tgattgtggt tcctctgcag atgacgatga ttccgattct tcgattattc
     2941 aacaagatag ggcttacggg cagttttttt gggatatggc ttgctcacac tgcctatggt
     3001 ttgccttttg ccatatatct cttgagaaac tttattgttc agcttccccg atctctgatc
     3061 gaatcggcac agatagacgg ggcctctcat ccaattatat tcttaaggct cgtattgccc
     3121 acgtcgattc cggcaatctc ctcgttggcc atatttcaat tcatgtgggt ttggaatgac
     3181 ttgttagtcg ctctaatcta tcttggagga accccaaacg ttgcgcccat gacggtaaca
     3241 ataagcaaca tggttaattc ttatggcgga ggctgggaat atttgacagc agcagccttt
     3301 atctcgatgt tgttgccgct tatcgttttt tttgcactgc agaagtattt tgttcgtggc
     3361 atactagccg gatcagtcaa gggatagggt acattcatat gaaaaaacag tcggtttcgg
     3421 catcggatgt tgcaaaaatg gctggcgttt ctcggacaac agtctcattc attctcaata
     3481 acactcaggg caagcacata agcgaagcaa caaggcaacg tgtcttaaaa gcagcggagg
     3541 ctcttcaata tgtccccgat atgaacgcta taagtctggc caaaaaagcc cattattcaa
     3601 tagggttctt tatatgctat tcaagagcta ttttcagcga tgcctatctc tttcgactaa
     3661 tcgaaggcat cacattggtc ttgaataaac atcattgcag gttggaacta cattctcttc
     3721 gaatgacaag aaggaactat ctcgatctgg tacattcctc gaagctagat ggtcttatcc
     3781 ttatgaatac gcacaccgac gatcctggca taaaggaatt ggaagaagca aagattcctt
     3841 ttgtggtaat aggctctata gagggtcttt ctgttcctca ggtcgacatc gataatgttg
     3901 cggctgcgag agagatggtc gattaccttg tctcgctcgg ccatagaaag atcgcaatga
     3961 tagcgcatgc gccattggag ttttatgctg cggcaaatag actggaagca taccggcaag
     4021 tattgaacga aaatggaatt cctattattt caaaatatat cgaagtagcg gatttttccg
     4081 aggaatctgg gtacagagca atgcagagat tgctctgcct cgaggataga ccttctgcgg
     4141 tgtttgcagg taatgacatg gttgcctatg gggctatgca agccatcatc gacgaaggct
     4201 tatccattcc tgaagatatc tctattgcgg ggtttgacga tgacttctta tcgagatatc
     4261 tcaatccttc tttgacaacg gttgcgcaac ccagcgaagg tttggggcaa gcggccgcaa
     4321 agctgctcat tcaggagata atatctccga ggaagtctgg attgcgagag agagactata
     4381 tcgttctccc caccatggtt gcaaaacgag aatcctgcag gagaatttga gatatgtcca
     4441 gccacgatgc ctggaaaatc acagaacctg gttttagtat tgatcatata gatacttacg
     4501 agactctatt tgcgcttgcc aatggcaatc ttggcctgcg tggcgatttc gatgaaaagt
     4561 accctgcgtt ccataagggg acatatatca atggtttttt cgaaaaagag ccgataactt
     4621 atggtgaaat agcttatgga tacgcagaaa accatgagac aattctgaat attcccaatg
     4681 ccaagtacat tgcgctcttt gtagatggcg aggaatttcg actcgatgcg attgagccct
     4741 cacggatcga agactatcaa cgatatctcg atattcgcaa tggagtactt gtacgcatag
     4801 taagagttag gctttcaagc aatgtgctca tcgaaataag atccacgcgt cttgtcagct
     4861 ttcttcgtga gagcatagca gctatcaagt atgaggtttg tcttttagaa accccaaata
     4921 gatggccatt tgtagtgcgg ctagagagtt gtatagaagg aagggttttt aacaggagag
     4981 ctgaaggcga tcctcgtgtt ggctccaaat ttcagcgcga tgcctatgcc atcgatagac
     5041 tcgagtatga taattcggag gcgagaattc tcatcacgac tcagttgagc agacttgcgg
     5101 ctaccgctca aatcagacat gtattttctg gaccaagaag catgcaagtt gaaaccgcaa
     5161 tcgaaggctc aacggtaatc catggttgga atggaacggt cgaggctggg cagaagtttt
     5221 cgcttgtaaa atacatggca taccagatgg ctgcgcttga ggacgcgggc tcatgctggg
     5281 cagaggctca ccaagagctt attcgagcgt ctcaggatgg cttcgaggtg cttttcaacg
     5341 agcagcggag ctttctttcg gaattttggg aacgcgctga tgtggttgta gagggagata
     5401 gtaaggttca acaggcgctc agattcaatc tttttcatgt gcttcagtcg actggcagaa
     5461 aaggcaagac cggtcttgcc gcaaaaggcc tgacgggaga aggctatgaa ggtcattatt
     5521 tctgggatac cgaaatatat gttcttcctt ttctcatata tacgatgccg gagatcgccc
     5581 ggaagctcct cgaatacaga tattctattc tcgataaggc cagacagcgg gctaagatta
     5641 tgtctgaaaa aggagctctc tttccctggc gcacaatcga cggagaagag gtaagtgcct
     5701 attatccggc tggcacggcc cagtaccata taaatgctga tatcatatat gcgatggaaa
     5761 aatactgtaa ggctagcaac gatatagagt ttttgtatgg accagtagcg gaagtagccg
     5821 cagagactgc acggctttgg atctctcttg ggcattattc cgaagataag cgctttcgaa
     5881 tagatgaagt aaccggtcct gacgaataca cagttcttgt cgataataat tgctatacca
     5941 atctcatggc caagaacaac cttgcattcg cagcgcgagt cttgaaggaa atgcaagaga
     6001 atagatcgca ggctttcgag gcgctgagaa aaaagatagg gctgctcgat agcgaaatcg
     6061 gattttggga gtctgccgct gaagccatgt atatacccta tgataaaaag actggtattt
     6121 atgctcaaga tgattcattc tttgagaaag aacgctggcc ttttgatgag acaccagcgg
     6181 acagatatcc actgctcttg cattatcatc ctcttgtaat atatcggcac caggtactca
     6241 agcagcccga cctcgtgttg gcgcaatttc tattgtcaga aaaatttagc cttgcagaga
     6301 agaaaagaaa cttcaagttt tacgagccac ttaccacagg cgattcgtcg ctctctcatt
     6361 gcattcaaag cattatggcc tcagaaaccg gagacgctaa agccgcgttg gactatttta
     6421 tgaagactgt gcgcatggat ctcgatgatg tgcatggaaa tagcagagat ggcatccata
     6481 ccgcagctat gggaggcagc tggctttcgg ttgtgtatgg gtttgcggga ttccgtgata
     6541 gagcctctgc aggagatgag gcgcttttcg atagtcaggc ggccgctgtt gatcaagcac
     6601 cttctgctga tcgggcggtt gcagatgtta aagcgtacta ttcattcaat ccaaggctcc
     6661 ccgagggctg gcaaaggctc tcattttcgc ttcagcttgg gagccagagg ctcgatatcg
     6721 acataaaaaa agacagggtg cgctatgcgc gagaaactga attccaggcg cgggttcaac
     6781 ctagtagcga ggcctcgaga gaggcgctcg ctttcaaaca taagaaagag cttattgttc
     6841 taaagcctgg cgaatcagtg gagatctcgc tcaagcctgt ctttagggca gcgatcttcg
     6901 atctggatgg cgtagtaacg gatacggcgc gctatcattt tcaagcatgg aagaagcttg
     6961 gcgaagagca tggctggcag gtcgatgagg cgctgaacga aaagctgaaa ggcgtgggca
     7021 ggatggaatc gctcgatatc attctcaagg agaatgctat cgatctacct gcatcgcaga
     7081 agattgagct tgcggagcgc aagaattctt actacaaaga gtttctcaca aggctcactc
     7141 ccgaggatat tctcccggga atgaaatcct tgctacttga gctgggaaat catggaatca
     7201 agcgcgtcct tgcctcagcg agccgcaacg caccaactat catcgagcgg cttgggcttg
     7261 ggccgatccc agggcagttt ttcgatgctc ttgctgatcc caatgcggtg acatgcggaa
     7321 aaccagatcc tgaattgttt ttgcttgcag ctgaccttgc aggcgctagc cctgaggact
     7381 gtgtggggat cgaagacgcc caagctggga taatcgccat aaaagctgct ggtatgaaag
     7441 ctcttggagt aggggaagga ttgaccggcg cggatatcgt cgttgaatcg acctcggaga
     7501 taagcttcca aatgctcgaa tcgctcttta ggtcgaaatc ataatcaatc tcttatatct
     7561 aatgaaatta aatatgttta tcccctaagg ccttatcaat cctgattatt ccaactaatt
     7621 tgcataatct atccttaggc cctccaaaac ttccttgtaa aaagcgcaag caccgtatac
     7681 acctcaagcc ttccgacaag catatccagc gaaagccaga gctttgccca ccaggggaaa
     7741 agcgcatagt taaaggctgg cccgaccttt cccagtccag ggccaatgtt gccaagatta
     7801 gcgatgactg ctgttatatt cgttagtaaa tctacgccac taaaggctat tacaagtacc
     7861 gaaagaaaga aaaaagcaaa gtagagaaaa acgagacccg atatatcata gatgacattt
     7921 ttcctcagcg ccctgtttcc caaaaaaata gtgtatacac ctcgtggatt tgtaacatag
     7981 cgcatctcgt ttactgccat tttgaagagc actacaaggc gaatcacttt gaccccacct
     8041 cctgtggagc cggaacatcc gccgataaac atcaaaaaga aaagtacagt ctgagatagc
     8101 tgtggccatt gcccaaaatc cgtggaagca aaacctgtgg tcgtaagaat cgaggatact
     8161 tgaaaagatg cgtggcgcaa cgaatctccg atatttccaa atactcccga tacgaatagg
     8221 tcgactgcaa taattactga tgatatagca aaaattccta agtaaaccct aaattcggtg
     8281 tctagtaaga ttgtgctcat tcgtcctttt aggaaatgat agtgcagtgt aaagtttgtg
     8341 cctgcaagaa acatgaaaat cgtaataatc cattggatga atccgctatt gtaataggca
     8401 atatttgcat tcttggtgcc gaagcctcca gtggccatgg tggcaaaact atgacagata
     8461 gcgtcgaaaa gtggcatgcc acccaagaca agaaggataa tttcgcagac tgtaaatcca
     8521 cagtaaatca accatagggt ttttgcggtc ttggaaatac gcggggtaat cctatccacc
     8581 tcaggactgg gggactccgc ctccataatt cggattgcgc taaagcccag actaggcaga
     8641 atagcaacca aaagcacaac gattcccatg ccaccgagcc agtgcgtggt tgctcgccag
     8701 aggagcagag gtcgaggtat tatctcaata tctgtcaata tgcttgctcc tgtcgtggta
     8761 aagcccgata ctgtctcaaa gaatgcgtct atataagaag ggatataccc ggagaaggta
     8821 aaaggaatgg cgcctaaagc cgaagaaaac acccagctaa aggccacaaa aaggaaacta
     8881 tctttgtttg taagaaaggg tctttcatca tgccttgtca ttaaatacat tactataaaa
     8941 aatgctaggc tgatgatggt aggtatgaag agcgaaaagc tcacccgcca ggataggtct
     9001 tcgacaatag agtaaattcc gcagatcaag ctaaaaatcg agacaatcaa aagaataatg
     9061 gaaagcattc tgagaatcga tttgaaatgc atatcatgcc ccaaaaatgc tctcaagctt
     9121 gcctatgtgt tcttttttga taattagtcc cacaagatcg ttttcgaata tctttgtaga
     9181 gcccgtggga atgcaggttt ttcctctatg gatgacaaaa caaatcagca atccctttgg
     9241 aagattcatg ttgcgtatct ccacttcgag ctgctcgaaa gagtccgtaa tctgaatctc
     9301 gatgagctca tattcattat gtggaaagct atagagtctc cttattttgg ccttccttac
     9361 cttgtcgagt attgcgcccg acataacgat tttttcattc acgacgatgt tgatatcgag
     9421 caaatagaga agcggagtgt agagatcgtt tgtaatgagc gcaaggcttc gctttgctcc
     9481 tgcttcttgg gctagaagag cgctcagaat atttatgctc tgttcagatg caaggcagag
     9541 aacaatgtcc gcgcttccga tgttctcctc cctgatcaaa tgctcatcgc tcaactgatg
     9601 atttattgct tctatatcgg ggaaagtagc cgagagaatc tttaagctct ctcgatctcc
     9661 gtatattacc ttgatttttg gatttccaag caatgagagg gctcccctat cgtacccctt
     9721 atcgcgctgt gaaccggaaa aagcagaaat ctgcctcatt ccgagctcct ttagcaagag
     9781 tgtcgtaaga gtgcctgtac caaggattac gatagagctg aatttcctga gagtgtcttt
     9841 gcatggtcca aaaagtctat cgaggctaga gggctcgcct aggatatagg ccgcatcttc
     9901 ttcttcgaaa acgaaatcac cgcccggaac gattagttct tcttcgcgca gcactgcggg
     9961 taccataaaa tccgccccaa tgctagtgcg gatattggcc agcgattggt gtacaaacct
    10021 tggctctttc ttacatagaa tgcgtcggag ctgaattcct gcttctttta catcgataat
    10081 ctcgggcgac atgccacgga aaatggtgcc cgcaatactc tcggcagttt cggcctcggg
    10141 gttgataata taatcgacac ctagaatctt ctttcctgca ttcttaaagg atgcaaaata
    10201 agggttgcgg gcgcgggcta tggttcttac gtctttgaag ttctcggcaa ctaagccaca
    10261 ggagacgata ttggcttcat cgctgccggt acaagcgata aaccagtctg cgtcttctac
    10321 accagcagcc ttaagagtct cgatgttttc gccatcgcct tcttggacga tgcaatccag
    10381 cttgtccgcg atcgattttg ccgcattcag atttttctct attataacga catctttgtt
    10441 ttccgctatg agttcgctgg caataagaga accgagcaaa cctgcgccaa gaatagctat
    10501 tttcatcgat gaaaagtata tgcgaccgag tgctattttg acaatgacag ggctcggatg
    10561 aagcgcttgt tgtatgccca acaattgggc ggcgccaaag atcgaaaaag ccctataaat
    10621 aggcgcatca acatcgacat attgagcctc attcgcattt tgcatactat aatgcgacag
    10681 gagcctcagt tgagaaaccg tttgggcaaa gaaaagcaga ttcctctatt atatttttta
    10741 ggcactattt tagccgggag cttccttttg gtgttgccaa tatcgagcga ggaaggcaac
    10801 ctctcatacc ttgatgcact tttcgaggcg accagcgcgg tctgcgttac cggtcttgct
    10861 gtcgttgata cgaccaggct ctctcgattt ggccaaacag tcatcctttt cttgattcag
    10921 cttggaggct tgggtatagt taccttttcc accttataca tttttatgcc aaggcgaaca
    10981 gtgtcggtga caactcatgg tatgataaag gattataccc tatcgtcggt tgaatatcgc
    11041 acgcgcagaa cgatccgaga gattatagca tggaccgcca gtttcgagct catcgctgct
    11101 tttgtgatct ggccaacgct tgcaaaaaat ggttactcct tctatgacgc gcttttccat
    11161 gcaatctcag ccttctgtaa tgctggattc tcgactttgc gatcgggcat ggagagct
//
LOCUS       DAOMZL010000055        10709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_5770_length_10709_cov_10.757556, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000055 DAOMZL010000000
VERSION     DAOMZL010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 10709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10709
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5770_length_10709_cov_10.757556"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1908
                     /locus_tag="PLQ30_05635"
     CDS             <1..1908
                     /locus_tag="PLQ30_05635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW01962.1"
                     /translation="VNDGMRAANGRWIFIVDSDDRLPNRAIENITIHLPSIEAIPNVA
                     GLSGLKADFSGQIIGESFPAKLKFCDAISLTYRYGIRADKADVYKKEVLASFPFPEFE
                     GERFLTEAVIWYRIANAGYALLLTNEVLYECEYRGDGLSAHSLELRIANPIGTLLFYK
                     EIVGYDLPYRAKLREASNYMRFCKHYRRSSCFDPRDLEQVSHAYLPPKLRIPASIIGN
                     AAYLYDQLTLARSRNTRNRCNVSASMPFDTKKPIRVLHVINSLSQGGAERFLASLLPR
                     LKAQHIENMALVLNAESAIYASVLEEAGIPVYKPGPQGFVSDSNKKRPKKRAKEPQLY
                     SPSRVFDVLRAIRCLRPDIVHAHLAPSFHWCALASLFAGKPVYITTEHASKNRRMSIP
                     FFKWIEKACYARYASIICVSPSVAQALSEWLGIRSDKLQTIVSGIDIEQFSNSSKPAP
                     EVVRFLGGRTGIAMTARFVPVKDHPVALEILDRLPERYALILIGDGPGLSYIQDLAAK
                     MRLEQRCLFLGSREDIPNILAACHLYIQTSRQEGLGLAVAEAMAAGLPVVVRKTSGLF
                     DLVDGAGLFFEARDIEQAVVQIISLENRDLREKMVLAESERIKYLSIERVAYEYGSHY
                     RKILRGKKEAK"
     gene            1905..>2443
                     /locus_tag="PLQ30_05640"
     CDS             1905..>2443
                     /locus_tag="PLQ30_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01963.1"
                     /translation="MKTRDFLLATSLRFLPFLFALAFSFSGYTNIAIFVILFLSFWYL
                     ILNWQIVIRDRWALFVIGFFLAIAAKDGIMFALGKGSFVAFAKSGSRPVVLAGCSAML
                     HAHSRKDIERALMDGFWVTFSYLLIIFILMRFRKLPIIYNINTFGMTGIWAAILTGTN
                     RWQNKIKNSRLEALAILISV"
     assembly_gap    2444..2543
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2544..2907
                     /locus_tag="PLQ30_05645"
     CDS             <2544..2907
                     /locus_tag="PLQ30_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01964.1"
                     /translation="PLIGWGFTTEADNIRLVSSYMIDTPFYDAFAKTGLGPHNSFLAM
                     FFENGVIALAGYITLLLARIRAMRPPLTMFDVSLLAYLVFMSLDAMAPGGLTVLGYFL
                     GICILEVPERSQEDKENR"
     gene            2904..3998
                     /locus_tag="PLQ30_05650"
     CDS             2904..3998
                     /locus_tag="PLQ30_05650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW01965.1"
                     /translation="MRVLHLVTRSDSGGAQCMIATLAALQSQSGHVVAVASGLEGKGL
                     AWHSLPAAIEKLPVKHLKRNIAPWDDLLALSEIRHICMKWKPDILHVHTSKAAALTRI
                     AINYNRIPIIYTMHGYDQLKIANKKLLFIDKMLSPRTSAIVAVSRYDLGRMRADNYHP
                     ELIANGVNAQSNPPSDASELLLALRLLENLRRQKSLIGLMIARPSSPKRPELVREAAR
                     LLKGLCSIVWIGGNARSDDPGAFYALGPIPDAGRLIPFCDFVLLPSDHEGLPMTLLEC
                     LAAGKPFIASNVGGIPELIGNEYFEIAPGFAVENSPEDFASAICSLAHDTELRNIMGE
                     NGKRLWERFYTAETMAAEYESLYNHYKQIE"
     gene            4011..5642
                     /locus_tag="PLQ30_05655"
     CDS             4011..5642
                     /locus_tag="PLQ30_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01966.1"
                     /translation="MHLQKRVSVALPHHADKTMLFILIMLCAVLAFNAPNIYAQDYPT
                     QEEPKQLPYIAGFEFHLKGVRETAVLRYLDMQIGRRFSSHSELDSFIAEKQRLLQENR
                     VFDEEATIWYEQRNPSDPEAITIIVSAYATWTSLAVPIPKYTEDDGFFLALRYKDFNF
                     LGMLEPLVFSLDHYFLNGNTTIGAEFGYSPHFINAQWQFALASSVDIGQDLDLAFSDT
                     AISLSTEYPLSEVPEYWKLVPSLSYTYTKSSKDHVGIAGLSLAKPIFLLFPGNIEFQA
                     SYKQENYAGIRSYFAITEAVFSLRIPLFSVNSASMLAFAPSTGVSFSYDIENGRVYDA
                     GLCASGSLGYNNIKWKGNFRKGSSISLSGSYMFHVGPKHTTRPLDLLLSTELSAFYSW
                     FNLLGIDFRVLGRWDAGWSLYDDMSYYDDLDASDYLRGVRGPKYGDIAAIVSLQIPVN
                     FAQGKFFQWEAMEAEIFLIPFFDLGYVRTNPAKPIWSMDNLLMGGGLDIVIFPEYARS
                     FTYRLSLGYNIGHFLETKTLNWSDTEIWLGIGLHL"
     gene            5615..6553
                     /locus_tag="PLQ30_05660"
     CDS             5615..6553
                     /locus_tag="PLQ30_05660"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPW01967.1"
                     /translation="MAGDRASSVIEGSVAIESARMGNTLRAVAYHAVPQSSMSSFSRQ
                     LDYFAKHYVPLDAEGFTQFLAQGSCQSFGMLENNGHYSANTTISNWQDRARHRPGLII
                     SFDDGLIEQYEVAAPLLEARGMRGWFFVPSALLDLEQEFQQDFCALHDISLRRKEARK
                     EKTQHSLVSNRIAMGLNEIKDLARRGHVIGCHTAHHVRMRPTLDTAILFDEIVEAKKE
                     LEMRLGYPVNAFAWVGGEPDTYRADALQMLCEAGFSWIFTTQSSLFRVGGDTRAIHRT
                     VLEPDLPFPLFLAKINGLSDLMHCGRRKASLNAMRL"
     gene            6758..8248
                     /locus_tag="PLQ30_05665"
     CDS             6758..8248
                     /locus_tag="PLQ30_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01968.1"
                     /translation="MLYGRNLLFAQTRYSPATLQWRIALNINLEYTLRSEDIRLEPPV
                     SQYPSAAYSNGIDIQRNYFGQLPFAVLSIEAGTALNSQGSDQRSYNDQGIGVAAEVDL
                     RHGWTGDYFPVHNLPLKGTTPDIEANIIARSVLYWKSPMLDIGFGRDRLDYNGILEGG
                     FLPSARIPYYDALRAQGRLGCFTIDWLVSTIQAVEAWDKNDITPEVGYGFEDQPGTPT
                     IIVEAMNRFSWHFGAFSVGVTDHAMMSRLNNHFVLTDFFPIGSRHQASVAQTNNSMVF
                     DASWEPFDGLAIAAQLGLDDIDLNVLVEGSDTGSPTIPAYLAAVRYDGEVRTLPYAVY
                     LEAGSTHWLWGNYDGSQAWPTIGDVNYFARFIYRFPRYLGGSILLPLTSPYGPGVAWI
                     QSHGELIIPIGSENDRHPLSFGVGYRFLFLDMNTDANLITTQVYNNSTTPAAPHWQSF
                     FISLPLRLSAGLFSLDIAPSFVGSGGSTGFELDIALSGTIAFATVH"
     gene            8333..9910
                     /locus_tag="PLQ30_05670"
     CDS             8333..9910
                     /locus_tag="PLQ30_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01969.1"
                     /translation="MKKLFLPAIVILLFLIFSGIFTLSAQQLSQSAAQQPGQSAAQQS
                     EELAEQGLLFTLATELPYRAGTYREEIEARGLAPNASISLQYEKPHIGLIAAIDLTSD
                     QVYGPWLADSPTGALGDLNFLIQEAGMRISGGSFNLKAGRLRHFDVVDSPYSLFINAN
                     GIAAPMIDFVYRDSRFFYESRFISLTLDSAMHTTAWPKASYTGFPDRGANFKVFGLSF
                     GRLTIGFEDSSVYSGRAFDLEYFLSPMPQYFTQYVRGTAGRPWYANYDDNYNAGLFVT
                     WKEPGAYDLYAQAFVDDLGMLGLFGWTNNPWQIALALGGRIKTPKGTFGLHVAGATKY
                     TFEPGDMRNASTENQIQSSYGYTYFPETRFEYEWRSGYSTSYKAIAPELNLIGYQYGE
                     NNIALRLDWSKAVNRFDCDAFFEFRLSGSNSPANPWHDLWIDPQVAFTWLDDPVLEKR
                     FTISARAITAREPWQFFAKATGVLALDALELRDPSGVPSTQTEVDQKVQIYSPVAGNT
                     KLLGELTFGVVLRLGIQ"
     gene            9907..>10709
                     /locus_tag="PLQ30_05675"
     CDS             9907..>10709
                     /locus_tag="PLQ30_05675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW01970.1"
                     /translation="MSEALKASIYRDLDTPAAIWDSLAQDSRIYAFQTRHWLSVWQNT
                     IGNAHGVVPCIIVFEKGQNTALFPLGKKKVRFGTSSHTMLIWLGYGVSDYAAPIFSNE
                     AQFDMTELFAHLRSIAKKEHCCAVYLDRIPKFWADGTAVLFVRALLALPFGHRLHYSA
                     HAMHLSQDPLLRLNAKERAHLRRAERRLSEAGTLSFIIAKDKSLRKHLTCSMIAFKQA
                     RFRQMKVHDNFVDPAFGDFYIQASQDEHIPLHICALMLNERPLAVHWGL"
BASE COUNT         2632 a         2532 c         2562 g         2883 t
ORIGIN      
        1 gtcaacgatg gtatgcgagc agctaatggt agatggatct ttatagtcga ttcggacgat
       61 aggcttccca atagagcaat cgaaaacata acaattcatc taccctctat agaggcaatt
      121 ccaaatgtgg ccggattaag cggccttaag gcagatttta gtggacagat tatcggtgag
      181 tcttttcctg ccaaactgaa attttgcgat gccataagcc tcacctatcg ctatggaatt
      241 agagccgata aggctgatgt gtacaaaaaa gaagtactgg cgagttttcc atttccagaa
      301 ttcgaaggag aacgatttct gaccgaagcc gtaatctggt atcgtatagc gaatgcaggt
      361 tacgccttgc ttctaactaa tgaagtcttg tatgaatgcg aatacagagg ggatggacta
      421 tccgctcata gtctggagct tcgcattgcc aatccgatag ggactctgct tttctacaag
      481 gaaatagtcg gctatgactt gccttataga gcaaaactga gagaagcgtc aaactatatg
      541 cgtttttgta agcattatcg gcgctcttcc tgcttcgacc caagagatct cgaacaagta
      601 agtcatgctt atctacctcc aaaactgcga atacccgcat ctatcattgg taacgccgcc
      661 tatctctatg accagctcac gctggctcgc tcaagaaata caagaaacag gtgtaatgtc
      721 tcggcgagca tgccatttga tacaaaaaaa cctattcgag tactgcatgt cataaactcc
      781 ttatcacagg gcggagcaga gcgctttctt gcatctctcc ttccaagact aaaagcgcag
      841 catatcgaga atatggctct tgtcctcaat gcagagtctg cgatatatgc ttctgtgttg
      901 gaagaagcag gaattccagt ttataagcct gggccgcaag gctttgtttc cgattccaac
      961 aagaaaagac ctaagaagag agcaaaggaa ccgcaactct attcgccctc gagggtcttc
     1021 gatgtgctta gagccattcg ttgcctgcgg cccgatatcg tccacgctca tctggctcct
     1081 tcgttccatt ggtgcgcact tgcaagcctc tttgctggaa agcctgtcta tataacaacc
     1141 gaacatgcca gtaagaatcg gcgtatgtcc attccttttt tcaaatggat cgagaaggcc
     1201 tgttatgcgc gatatgcttc gattatctgt gtgagtcctt cggtggctca agcgctttcg
     1261 gaatggcttg ggattcgttc cgacaagctc caaaccatag ttagtggtat tgatatagag
     1321 caattctcga atagttcgaa accagcgccg gaagtagtcc gatttcttgg aggaagaacc
     1381 ggaatcgcta tgacagcgcg atttgtacct gtaaaggacc atcctgtcgc gctcgagatt
     1441 cttgacaggt tgccagagcg ctatgctctc atattgatag gcgacggtcc agggctatcg
     1501 tacattcaag atctagccgc aaaaatgagg ctagagcagc gctgtctatt tctcggctcg
     1561 cgcgaggata ttccgaatat tctcgctgca tgccatttat atatacagac ttcgcggcaa
     1621 gagggacttg gcttggcggt tgcggaggca atggcagccg ggcttccggt agttgtgcga
     1681 aaaaccagcg gtcttttcga tctcgtagat ggtgcgggtt tgtttttcga agctcgggat
     1741 atagagcagg ctgtggtgca aataatttcg cttgagaacc gagatttgcg agaaaagatg
     1801 gtgctggccg agagcgagag aataaaatat ctttcgattg aacgtgttgc gtacgaatat
     1861 ggatcacatt accgcaagat tctgcgggga aaaaaggaag caaaatgaag actagagatt
     1921 ttctcttagc cacaagcttg cgttttttgc catttctttt tgcccttgca ttcagcttta
     1981 gcggatatac taatatcgcg atttttgtaa ttctttttct aagcttttgg taccttatct
     2041 tgaattggca aattgtcatt cgcgatcgat gggccctatt tgtgataggc tttttcctgg
     2101 caattgcggc caaagatggt attatgtttg ctttggggaa aggcagcttc gtagcatttg
     2161 ctaagtcggg gtctcggcct gttgtgctgg caggctgcag tgcgatgctc cacgctcatt
     2221 cgagaaagga catagaaaga gctcttatgg atggcttttg ggtgactttc tcttatttgt
     2281 tgatcatttt tattcttatg cgattcagaa aattacctat aatctacaat ataaatacat
     2341 ttggaatgac ggggatatgg gctgcaattc ttacgggaac aaatagatgg caaaacaaga
     2401 taaaaaacag cagactggag gctctggcaa ttcttatctc ggtnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnngcccttg attggctggg gatttactac ggaagcggac
     2581 aatatccgcc ttgtgagttc ctatatgatc gatacaccgt tctatgatgc ctttgccaaa
     2641 acaggcctcg gtcctcataa tagctttctg gcaatgttct tcgagaatgg tgttattgct
     2701 cttgcaggat acataacatt gcttcttgct cggataaggg caatgcgacc gcctctgaca
     2761 atgttcgatg tttccctttt ggcctatctt gtctttatgt ctcttgatgc gatggctcca
     2821 ggagggctga cagtactggg atatttcctg gggatatgca ttctcgaggt gccggaaaga
     2881 tctcaagagg acaaggaaaa tcgatgaggg ttctgcattt agttacccgc tccgattcag
     2941 gcggcgccca atgtatgatc gcaacacttg cagccttaca gagccagtcc ggtcatgtcg
     3001 ttgcagttgc ttccggcctc gaagggaaag ggctcgcctg gcattccctt ccggccgcta
     3061 tagaaaaatt gccggtcaag cacctaaagc gaaacatagc gccatgggac gatctgttag
     3121 cgctatcaga aattcgtcat atctgcatga aatggaagcc cgatatcctg catgttcata
     3181 cgagcaaagc agctgccctt acaagaatcg ccataaatta taatcggatt ccgataatct
     3241 atacgatgca tggctacgac cagcttaaga ttgccaacaa aaagcttctt ttcatcgata
     3301 aaatgctctc tccaaggaca tcggcgatcg ttgccgtttc tcgctatgat cttgggcgca
     3361 tgcgggccga taattatcat ccagagctta tagcaaatgg cgtaaatgct caaagcaacc
     3421 cgccatccga tgcttctgaa ttgcttttag cattacgcct tctagaaaat ctgcgaagac
     3481 aaaaatcgct aatcgggcta atgatcgcca ggccctcttc tcccaagcga cccgaactgg
     3541 tccgtgaggc tgctcgcctg ttaaagggtc tttgcagtat agtatggata ggtggcaatg
     3601 ctcgatccga tgatccaggc gctttttatg cgcttggacc tattccggat gccggcaggc
     3661 tcattccttt ttgcgatttt gtcctcttgc catcggacca cgaaggtctg cccatgaccc
     3721 ttctcgaatg tttagctgca ggcaagcctt ttatcgcctc taatgtaggc ggcattcctg
     3781 agcttattgg gaatgagtat ttcgaaatag ctccgggatt tgctgtggag aatagccccg
     3841 aggatttcgc ttcggctatc tgtagccttg ctcatgacac cgaactgcgc aacataatgg
     3901 gcgagaatgg gaaaaggctc tgggagagat tctataccgc cgagacgatg gcggctgaat
     3961 atgagtcgct atataatcac tacaagcaga tcgaatagga agaaacagct atgcatttgc
     4021 agaaaagagt gtctgtggca ctgcctcatc atgccgataa aacaatgcta ttcattttga
     4081 ttatgctatg cgcggtactg gcttttaatg ctccaaacat ttatgcacaa gactatccga
     4141 cacaagagga acctaagcag cttccctata tcgcgggatt cgaatttcat ctaaaaggtg
     4201 tcagagagac ggcagttctt cgatatctcg atatgcaaat aggccggaga ttttcaagcc
     4261 attcggagct cgacagtttt atagcggaaa agcagaggct ccttcaagag aatagagtct
     4321 tcgatgaaga ggcgacaatc tggtatgaac agcgaaatcc atcagatccc gaagcaataa
     4381 ccattatagt gagtgcctat gcaacatgga cttctcttgc cgtacccata ccaaagtaca
     4441 ccgaagacga tggttttttc cttgcattac gctataagga tttcaacttt ctcggcatgc
     4501 tggagccact agtgttttcc ttagaccatt attttctaaa tggaaatact accatcggtg
     4561 cggagtttgg gtactcgcct cacttcataa atgctcaatg gcagtttgcg cttgcaagtt
     4621 cggtcgatat cggccaagac ctcgatctgg cattcagcga tacagcaatc agtctttcga
     4681 ctgagtatcc tctctctgaa gtacccgaat attggaaact tgttccttcg ctatcttata
     4741 cctatacaaa aagctccaaa gaccatgttg gaattgctgg cttaagcctg gcaaagccga
     4801 ttttcctgct ctttcccgga aacatagagt tccaggcttc atataagcaa gaaaattatg
     4861 ctggtattcg gtcatatttt gctattacag aagcggtgtt ttctcttcgc attcccctgt
     4921 tctcggtaaa ttcggcatct atgctggcat ttgcgccttc cacaggtgtt tccttttcct
     4981 acgacattga aaatggcagg gtatacgatg cggggctctg cgcatctggc tcgctggggt
     5041 acaacaacat caaatggaaa ggcaacttca gaaagggcag cagcatttct ttatcgggat
     5101 catatatgtt ccatgtgggg ccgaaacaca caaccaggcc cttggatctt cttctttcca
     5161 ccgaactatc tgccttttac tcctggttca accttttagg aatcgacttt cgggtgctgg
     5221 ggcgctggga tgccggatgg agcctatatg acgacatgag ctattacgat gatttggatg
     5281 cgagcgatta ccttcgcgga gttcgagggc caaaatatgg cgacatagcc gcgatcgtca
     5341 gtctgcagat accggttaat ttcgctcaag gcaaattctt ccaatgggaa gcgatggagg
     5401 cggagatctt ccttatacca tttttcgatt tggggtatgt ccgaacaaat cctgccaagc
     5461 ccatttggtc tatggacaac cttctgatgg gaggaggact cgatatagtg attttccccg
     5521 aatatgcgcg gtcgttcacc tatcggctct ctttggggta taatataggt cacttcctag
     5581 aaactaagac tctgaattgg agcgataccg aaatatggct ggggataggg cttcatctgt
     5641 aatcgaaggt tcggttgcaa tagaaagcgc aagaatgggc aataccctgc gggctgtcgc
     5701 atatcacgcc gtacctcagt cttcgatgtc cagcttttcg cgccagctcg attactttgc
     5761 aaagcactat gtcccgctcg atgcagaagg cttcacgcag ttcttagccc agggatcctg
     5821 ccagagcttt ggaatgcttg aaaataatgg ccattatagc gcaaatacga caatttcgaa
     5881 ttggcaagac cgcgcacgac acagaccagg ccttatcatc agcttcgatg acggtcttat
     5941 cgaacaatac gaagtcgccg cgcctcttct cgaagcccgc ggtatgcgcg gatggttttt
     6001 tgtcccgagt gcacttcttg acttggaaca agaatttcag caagacttct gtgcattgca
     6061 tgatatttct ctgcgcagaa aagaggctcg caaagagaaa acccagcact ccctggtttc
     6121 aaaccgtatt gcaatgggtt tgaatgaaat caaggacctt gcaaggcgcg gtcatgtgat
     6181 tggttgccat accgctcacc atgttcgaat gcgtccgact ctcgataccg ctatactctt
     6241 tgacgagatt gtagaagcta aaaaagagct cgagatgcgc cttggctatc ctgtcaatgc
     6301 atttgcctgg gtaggaggag aaccagatac ctaccgtgcg gacgccttgc agatgctctg
     6361 cgaggcgggt ttttcatgga tttttacgac ccagtcctcg cttttcaggg tgggtggaga
     6421 tacgcgagca atccatcgca cggtgctcga gcccgatctt ccattcccgc tttttcttgc
     6481 aaaaataaat gggctctctg accttatgca ttgcgggcga agaaaggcaa gcctcaacgc
     6541 catgcggctt tgaaagaggc tcatctattg cataagcgga atcaaaaggg ctatcaagtt
     6601 gactgccttc aaaaaggagg acgatgaagc catgaagcaa ccatgtggta tgtgggattg
     6661 tatgcggagt ggtatggggg gctgtatgcg ggccaaaaaa ctcagaccat ttctgctaca
     6721 tttaattttc tatatatttt tgttttcggg atctgggatg ctttatggaa ggaatctcct
     6781 ttttgcgcaa acaaggtatt cgccggcaac cctacagtgg cgcatcgcac tcaacataaa
     6841 cctcgaatac accctgagaa gcgaagatat ccgactcgaa cctcctgtga gccagtaccc
     6901 gtcggcggca tattccaatg gtatcgatat acaacggaat tattttggcc aattgccttt
     6961 tgcggttctg agcattgaag ccggaactgc cctcaattcg caaggatcgg atcaacgttc
     7021 ttataatgat cagggtatcg gcgtggctgc ggaagtcgat ctgcggcatg gttggacagg
     7081 agactacttt cctgtgcaca atctgccgct taaggggaca acgcctgata tagaagcgaa
     7141 cattattgcg cgatccgtgc tatattggaa atctccaatg ctcgatatag gctttggacg
     7201 tgaccgcctc gattacaatg gcatacttga aggcggcttt cttccttctg ctcggatacc
     7261 atattacgat gcattaagag ctcaaggaag gcttggttgc tttacaattg actggctcgt
     7321 gtccacaatt caggcagttg aggcatggga caaaaatgat atcacgccgg aggttgggta
     7381 tggctttgag gaccaaccgg gcacgcctac cataattgta gaggcaatga atcgattttc
     7441 ctggcatttt ggtgcgtttt ccgtgggcgt aacagatcat gccatgatgt ctcgtctcaa
     7501 caaccacttt gtgctgacag attttttccc gataggctcg cgccatcaag catcggtggc
     7561 gcagaccaat aactccatgg tgttcgatgc aagctgggag ccttttgatg gccttgccat
     7621 agctgcacag cttggtcttg atgatatcga tctgaatgtg ctcgtagagg gttcagatac
     7681 aggttcgcct actataccag cctatctggc ggctgttcgt tatgatggcg aagtaagaac
     7741 cttgccttat gcggtttatc ttgaggctgg ttccactcat tggctttggg gaaactacga
     7801 cggaagccaa gcctggccta ccattggaga tgtcaattat tttgctcgct tcatataccg
     7861 gtttccacga tatctgggag ggtctattct cttgcctctc acaagtcctt acggcccagg
     7921 cgttgcctgg atacagagtc acggcgagct cattatccca attggaagcg aaaacgatag
     7981 gcatcctcta agcttcggag ttggctatcg ctttttattc ctcgacatga atacagatgc
     8041 caaccttatc acgactcaag tctataataa cagcacaacg cctgcggctc cacattggca
     8101 aagtttcttc ataagcctgc ctttgcgcct ttccgcgggt ttgttttctt tagatatcgc
     8161 accgagtttt gtcggaagtg gtggttcgac gggcttcgag cttgacattg cgctctcagg
     8221 taccatagca ttcgcaactg ttcattagtg atacgaacct tgtgtatgca gtagactttg
     8281 ctgttaatcg atcgaactgt tatagactta atgatggatc gtaggaatat agatgaaaaa
     8341 actatttttg ccagcaattg tgattctttt atttcttatc ttctcgggaa tttttacgtt
     8401 gtctgcacaa cagctcagcc aaagcgctgc ccagcagcca ggccaaagcg ctgcccaaca
     8461 gtccgaagag cttgccgagc aaggtcttct ctttactctt gccacagagc tgccttaccg
     8521 cgcgggtaca tatcgcgagg aaatcgaagc acgaggcctt gcaccaaatg cctctatatc
     8581 tcttcaatat gagaaacctc atattggcct catagcggcc atcgatctca ccagcgacca
     8641 ggtctatggc ccttggctgg cagacagccc aacgggtgcg cttggagatc ttaattttct
     8701 aatccaggaa gcgggtatga gaatttccgg cggctcattt aatctcaaag ccggtaggct
     8761 tcgccatttc gatgttgtgg attcaccgta ctcgctcttt atcaacgcca atggtatcgc
     8821 ggcgccaatg atcgactttg tgtatagaga tagccgattc ttttatgaat cgcgctttat
     8881 ctcactcact ctcgattcgg caatgcacac gacggcgtgg cctaaggctt cctataccgg
     8941 ttttcccgat cgtggggcca atttcaaggt ttttggtctt tcttttggga ggcttacgat
     9001 aggctttgaa gactcctcgg tgtattccgg ccgagcattc gatttggaat actttttaag
     9061 ccctatgcct cagtatttta cccaatatgt cagaggcacc gcaggtcggc cctggtatgc
     9121 caactacgac gataactata atgcaggatt atttgtcacc tggaaggagc ccggggctta
     9181 tgacctgtat gcgcaggctt ttgtcgacga ccttgggatg ttgggactct ttggatggac
     9241 gaacaaccct tggcagattg cgctcgccct cggtggaaga ataaagacgc ctaaggggac
     9301 ttttggcctt catgtagctg gtgcgacaaa atacaccttt gagcccgggg atatgagaaa
     9361 tgcttccaca gagaaccaaa tacagagctc ctatggctat acctattttc cagaaacccg
     9421 ttttgagtac gaatggcggt ctggctattc cacaagttat aaggcgattg ctccggaact
     9481 aaatctcatc ggttaccaat atggcgaaaa caatattgcc ttgcgcctcg attggagcaa
     9541 ggctgtcaac agattcgact gtgatgcctt ctttgaattc aggctgtctg gctcgaatag
     9601 ccccgccaac ccctggcacg atctttggat agatccacaa gtggccttca cctggctcga
     9661 tgatcctgtg ctcgaaaaaa gatttaccat ttcggcccga gcaattacag cacgtgaacc
     9721 ctggcagttt ttcgcaaagg ccaccggcgt ccttgctctg gatgcgctcg aactgcgcga
     9781 cccaagcggc gttccatcaa cccagacaga ggtcgatcaa aaagtgcaaa tctattctcc
     9841 tgttgcgggt aacacgaaac ttctaggcga actcaccttt ggagtcgtct tgaggcttgg
     9901 gatacaatga gcgaagcgct caaagcctcg atttaccgag atctcgatac acccgcggcg
     9961 atctgggata gcctagcaca agattcacgc atctacgcct ttcagactcg ccactggctt
    10021 tcagtatggc agaacaccat cgggaatgcc catggagttg tgccttgtat catcgtcttt
    10081 gaaaaaggtc agaatacagc cctgtttcct cttggaaaga aaaaggtgcg atttggaact
    10141 tcttcccaca caatgctcat atggctcgga tatggcgtgt ctgattatgc tgcgccgata
    10201 ttttcgaacg aggcacagtt cgatatgaca gagctcttcg cccatctcag gagcattgca
    10261 aaaaaggagc attgctgcgc ggtgtatctc gaccgaattc caaaattctg ggctgatggc
    10321 acggcggttc tgtttgttcg agcactgctt gcgttgcctt tcgggcaccg cctccattat
    10381 agcgctcacg caatgcacct ttcgcaagac ccattattgc gtctcaacgc caaagaacgt
    10441 gcgcacctcc gcagagccga gcgccgtcta tcagaggctg gtactttatc ctttattatc
    10501 gccaaagaca aatcgcttcg aaagcacctt acttgctcta tgatcgcctt taagcaagcg
    10561 cgctttcggc agatgaaagt ccatgataat tttgttgacc ccgcatttgg cgacttttat
    10621 atccaagcct cgcaggatga acatattccc ttgcacatat gcgcgctcat gctgaacgaa
    10681 aggcctctag ccgtgcattg ggggcttag
//
LOCUS       DAOMZL010000056        10571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_5887_length_10571_cov_17.229270, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000056 DAOMZL010000000
VERSION     DAOMZL010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 10571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10571
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5887_length_10571_cov_17.229270"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1233)
                     /gene="argJ"
                     /locus_tag="PLQ30_05680"
     CDS             complement(16..1233)
                     /gene="argJ"
                     /locus_tag="PLQ30_05680"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HPW01971.1"
                     /translation="MKQSRGGVTAAKGFLASGVHVGIRKNKEKKDLALIWSEVPCVAA
                     AVYTTNRVKGQTLIVTQEHLADGRAQAIIVNSGNANTCTGEEGLKAARRMAELVSQYL
                     PVKTEDVVVASTGVIGQQLDVSVIETGMPDLVKGLSRGGSIDAREAIMTTDTMKKEIA
                     VIEELDGVPVTIGAMAKGSGMIHPNMATMLCFITTDCAIAEPLLRKALKEVVHVSFNR
                     VSVDGDTSTNDMVVVLANGKANNAFIDKEDDNYSDFRDALQFVCVELAKMVARDGEGA
                     TRLIECTVGGAASEEQAESFAKGIITSSLVKTAMFGADANWGRILCAMGYTRSDFDPD
                     KVDISFESPRGYIEVCKAGAPLPFSEEKAKKILNNQEVEIIVDLHLGEAEATAWGCDL
                     SYEYVRINGDYRT"
     gene            complement(1248..2297)
                     /gene="argC"
                     /locus_tag="PLQ30_05685"
     CDS             complement(1248..2297)
                     /gene="argC"
                     /locus_tag="PLQ30_05685"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPW01972.1"
                     /translation="MIHVGILGATGYAGLELTRILLQHPEVTALSASSVSFEGKRFEE
                     VYPSMINLQSDVALPMLEKAEDVICKADVIFSCLPHGHAEEAGQQCFEASKPFIDISA
                     DFRFGVYEDIFKKWYGVPYKYPKLHEYAVYGLPELKREIIKSAVLIANPGCYPTSAEL
                     GLIPAIQQGLVDISSIVITSMSGVTGAGREPSQTTHYPEAADAASPYKIGSHRHQPEI
                     DLLLSEIAGEQVQSVFTPILAPMNRGIVSMICFRLLRAVSDGELHSIYTEHYADEPFV
                     RVLPLGATASNRFVKFSNYCDISVHLSADKERAVIISTIDNMVKGAAGQAVQNMNILL
                     GFEETDGLRFIPPAF"
     gene            2738..4813
                     /locus_tag="PLQ30_05690"
     CDS             2738..4813
                     /locus_tag="PLQ30_05690"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPW01973.1"
                     /translation="MLSSGVFVAIITALAALGYSLITSSKIKKIPLSNPAVMRISGYI
                     EEGAHAFLAREYKAVGFIIPVVAVLLLCLNSGALKLQAVSFLIGSVLSALSGWFGMSI
                     AVKANARTAEAAQKGILPALRIAFSSGSVMGMTVVGLVLLGIGAILGLLLFMPDAEQI
                     LAQTIFPIVSGFSLGASMMALFARVGGGIYTKAADVGADLVGKVEVGIPEDDHRNPAV
                     IADNVGDNVGDVAGMGADLFESYAGSIVGCMVLGIAISASPELQQRLAILPLIAAGTG
                     VVASLIGTLFVHLSRAKRPQNALNAGSIGASIIAIVLLIPVVRFIIGDGRFSIGSREA
                     GWLGVYLACITGLVAGTVIGLITEYYTGSNSPPVRKIVKSCTTGAATTIISGLGVGMI
                     STVFPILVIGLAIGLSNRLAGLYGIGIAGLGMLLTLGIQLSVDAYGPVADNAGGLAEM
                     AEMEPEVRRITDSLDAVGNTTAAIGKGFAIGSALLTSLILIVAFLSAAELPVEMMNLA
                     NPSVLIGLFIGGMIPFFFSSLAMDAIYRAAFSMIEEVRRQFRAKPGILEGTDEPDYRT
                     CVDISTQTALKSMILPGISAIVAPVLAGFIGGPAVLTGLLVGATVSGGLLAIFMSNSG
                     GAWDNAKKFIESRSEEGKGSSSHRAAVVGDTVGDPFKDTAGPSLNILIKLMAVISLVL
                     TPVLKAVWG"
     gene            4917..5705
                     /locus_tag="PLQ30_05695"
     CDS             4917..5705
                     /locus_tag="PLQ30_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01974.1"
                     /translation="MDYEKHLYMIVHPNHSLIASQLEPDRFIKHYVQGSTRYFEGRLI
                     FVEVDPEFRNPYFDIDTAYSELKPHEDGRPKATKFIKSYRVLEHMDYDAFGKLYISNS
                     LGDYVELESKSHEPSTDAEEFRIMLEVNPVRFIVLTKLNYHDFAQFITDPRNTKGAPK
                     TAFTLLEFDSQQFMKEYEENPLIRCYVPGIHPARLAAAIEEVRNTPGKVVKGISLDCP
                     IDHISYKYLKEGFVFAEQNRPCKFYPYLDLGTIERKFYRFWKSM"
     gene            complement(5716..6120)
                     /locus_tag="PLQ30_05700"
     CDS             complement(5716..6120)
                     /locus_tag="PLQ30_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPW01975.1"
                     /translation="MELKEGIIAEKEIIVEEKYIASHLGSGGVPVYATPMMVLHMEET
                     SRLAVDHLLEPGKATVGAFIAVKHLAPTPKGMRVRIRSKLAKIEGRMLTFEVEAWDEI
                     EKVGEAEHIRSIIDIARFGERIEKKKALAASK"
     gene            complement(6146..7138)
                     /locus_tag="PLQ30_05705"
     CDS             complement(6146..7138)
                     /locus_tag="PLQ30_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPW01976.1"
                     /translation="MWLIILVYAGLVCTLTAVSYALMFRPKCKSLEFTRQASLERGEF
                     DENLLNLDWQEWSFASPYGYALKGQYLQGKKNAPAALFVHGITWSRYGMVKYMRPFIE
                     RGWNVAAFDLAGHGASIAPRRFYPSYGFYEKYDIKAGVEALHSLFSDARLYGLFGESL
                     GAASALQYAPLAKEASSRKIDFIVADCSFSSAWEELLEQYREIHIPNFIAWPSAHMTR
                     FFVRLLRGFDLKEASPSKAVMLTDMPIIFAHGMEDSYVPTIMSVQMASARMSNRIGLT
                     DLVLIPGAGHAVGILTDKARWLAAVDSFIDRVFYEEDPMRNSVVCNEYMDEKNK"
     gene            7340..8386
                     /gene="trpS"
                     /locus_tag="PLQ30_05710"
     CDS             7340..8386
                     /gene="trpS"
                     /locus_tag="PLQ30_05710"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPW01977.1"
                     /translation="MQKKRILTGDRPTGKLHLGHYVGSLANRVRLQDEYECFFIIADL
                     HTLTTRPEKENIERLPANIYSAVLDYLSVGIDPNKSVIYLQSAVPEVTELSLFFGNLI
                     TVPRLSRVPSLKEMAQNAHIEEMPFGLLGYPVLQAADILLPRAHLVPVGKDNEAHVEV
                     TREIARRFNYLYGETFPVPEVMVSEYGSLVGTDGNTKMSKSLNNAIFLSDDAKTVNKR
                     VMSMYTDPKRIRADIPGTVEGNPVFIYHDAFNQDKAEVEELKARYRAGTIGDVEVKER
                     LARVLNDFLGPIRERRAYYESQKGLVEEIIYEGTLRMRQEARITISMAKKAMGMSSIW
                     NRISRKAEERKKDA"
     gene            complement(8406..9839)
                     /locus_tag="PLQ30_05715"
     CDS             complement(8406..9839)
                     /locus_tag="PLQ30_05715"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPW01978.1"
                     /translation="MKLEASILRDFFLDSSLSDFFKETDIFYIDFLSHTAQSCTTLRS
                     FGYEDSDLLKVIEDFPVHESDRERANLQLQRIWSGQTETFIDAFRLASKDKSTYRWLR
                     FTIKVLLRNPNKRPRIIVGHVVDLDDLINSQEEIRERLVEIDTMRELFTAINKSLDFG
                     ETFSRTTSQLRRIIPFDRACAESLEDGRLVVVAGYGYNESDLSGLVFPAHGVDNPAVR
                     AIEERRIVICNDVRQSFPGFINATEDFATMSWLGIPLIYEKKVIGLLTLDNKELNAYN
                     DQHVRIASELSEYIAIALEHARRHQLVTRQAMTDKLTGLANRYGLETHGISLFQKAIE
                     EEHSIGVLILDIDHFKQINDTYGHAYGDVVLQTIAMTIRGQIRTRDYAIRYGGEEFVV
                     LLPDLGTREALIVAERLRNKIAQIKMEENKKMPTVSIGIYAAIPGTLDILHEYIRKAD
                     MALYAAKEAGRNRSRVWSISPEFYGER"
     gene            9881..>10571
                     /locus_tag="PLQ30_05720"
     CDS             9881..>10571
                     /locus_tag="PLQ30_05720"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW01979.1"
                     /translation="MKKPLNEISNSAFYARFNGWFETSSAIGLHLSNKRNISSVSKNL
                     RFLQRGLTGNRGLIASSYMDEWSILQPYLLYYWPISFYEVSAILYELKTRAVLPDINS
                     VLDLGSGPGPASFAAQTFGAMRASLVDKSKRALAMASAIAAKGRRKNLNLEIIEYCAS
                     IEDFCAPKGESYDLIIAANSLNELWADSPDGILRRRDLVLRFLPNLQPEGILIIVEPS
                     AHYTSIPLLELR"
BASE COUNT         2749 a         2534 c         2404 g         2884 t
ORIGIN      
        1 tagcctcatt gcatgctatg tgcgatagtc gccattgatc cgcacgtatt cataagaaag
       61 atcgcaaccc caggcggttg cctcagcctc tcccagatgc aggtctacga tgatttctac
      121 ttcctggttg ttgagtatct ttttagcttt ttcttcactg aaaggtaagg gcgcgcctgc
      181 cttgcagact tcgatatatc ctctcgggct ctcgaaagag atgtccactt tgtctggatc
      241 gaaatcgctg cgcgtatatc ccatggcaca gaggattctg ccccagttcg catccgcgcc
      301 gaacattgca gtctttacca ggctcgatgt aattatgcct tttgcgaaag attcggcctg
      361 ctcttcggat gctgctcctc ccacggtgca ctctatcagc ctggttgcgc cttctccatc
      421 gcgagctacc attttagcta gttccacaca gacgaattgg agagcatcgc gaaaatcgct
      481 gtagttgtca tcttccttgt cgataaacgc attgttagct ttgccgttgg caagaactac
      541 caccatatcg ttcgtggaag tatcaccatc cacgctgacg cgattgaagc tgacgtggac
      601 aacttcttta agtgcttttc ttagaagagg ttccgcaatc gcgcaatctg tcgttataaa
      661 gcatagcatt gttgccatgt ttgggtgtat catgcccgaa cctttagcca tagctcctat
      721 ggtaaccggc acaccatcta gttcttcgat taccgcgatt tcctttttca tcgtatcggt
      781 cgtcataata gcctctctgg catctatcga gccgccccta gaaaggcctt ttactagatc
      841 gggcatgcca gtttctatga ccgagacatc tagctgttgg ccaattacac cggtagaggc
      901 tacgactacg tcttcggtct tgactggaag atactgggat acaagctccg ccatgcgccg
      961 tgccgctttg agaccttctt ctccggtgca tgtgttcgca ttgcccgagt tgacaattat
     1021 cgcctgagcg cggccatctg caaggtgttc ctgagttact ataagggtct gaccctttac
     1081 tctattcgtc gtatacacag ccgcagccac acatgggact tctgaccata tcagtgctaa
     1141 atccttcttt tccttgttct ttcgaattcc gacatgaaca ccggaagcca gaaatccttt
     1201 tgcggcagtc acgccacccc gactttgttt catcgcatcc tccttaccta gaatgcaggt
     1261 ggaatgaacc tgagcccatc tgtttcctca aagcccagga gaatattcat attctgaacg
     1321 gcctgaccag ccgcaccctt taccatatta tcgatagtag agattattac ggctcgctcc
     1381 ttatccgcag ataagtgaac cgaaatatcg caataattcg agaatttgac aaagcgatta
     1441 gaggcagttg ccccaagggg cagaacgcgc acaaagggtt cgtctgcata gtgctctgta
     1501 tagatcgaat gaagctctcc atccgagacc gcacgcagaa gtcgaaagca tatcatgctc
     1561 acaatacctc gattcatagg agcaaggatc ggtgtaaata cgctctgcac ttgctctcca
     1621 gctatctccg acaacaaaag atcgatttcc ggttgatgcc tatgagagcc gatcttatag
     1681 ggcgaagccg catctgctgc ttcgggataa tgcgttgttt ggctcggttc gcgacctgct
     1741 ccagtcactc ccgacatcga agtgatcact atgctggaaa tgtcgacaag accttgctga
     1801 atagcgggaa taaggcctag ctccgcagag gtagggtagc atcctgggtt tgcgattaaa
     1861 accgccgatt tgattatctc acgctttagc tcaggcaaac catatactgc atattcatgc
     1921 agttttggat atttatacgg tacaccatac cattttttga atatatcttc atagactcca
     1981 aatctaaaat cagcagatat gtctataaaa ggcttcgatg cttcgaagca ttgttgaccc
     2041 gcttcctctg catgcccatg cggcaaacag gaaaatatga catcggcttt gcatataaca
     2101 tcttcggcct tttctagcat tggaagcgct acatcgctct gaaggtttat catagatgga
     2161 tagacctctt cgaatctttt gccttcaaag ctcacagatg aagcgctcaa tgcggttact
     2221 tctgggtgct gcaagaggat tcgggttaac tcgagcccag catagccagt ggctcccaat
     2281 atacccacat gaatcatggg catctccttt caataacaag tgtaatgaga aatgggatta
     2341 attgcacgta aaagtgcaag gttgtcagcg acgatagcag aatagataaa tacgacggga
     2401 atggaacgca agaaatgttc ttcgcagagt tctcaaagat ggtgaggaaa taggtatgag
     2461 aagtgagcat tccatgtgcc cctcccaata agaaaagtat ttcaataaaa atacaaatta
     2521 taaattttgt caaggataca gatggatagc agtttcatta attttgcata aaactgtggt
     2581 ttggccgata cacaattacc atgttttgat tttcataggc attgtcatat ctttgtttga
     2641 ggtcttgtat ttattttcaa tattgccaga tccgaaaaat cagccttata atgaaacgta
     2701 aagtacaatt tggcttttcg caatgaggag gtcgccgatg ctctctagtg gagtattcgt
     2761 tgcaatcata acagccctgg cagcgctggg gtattccttg attacgagct cgaagatcaa
     2821 aaagatcccc ctttccaatc ctgccgtaat gcgaatttct ggctatatcg aagaaggagc
     2881 tcatgctttc ctggctaggg aatacaaggc agtgggcttt atcattccgg tggttgctgt
     2941 tctccttctt tgtctcaata gtggcgcact gaaattgcag gctgtttcct ttcttattgg
     3001 ttcagttctg tccgcgttgt ccggctggtt cggtatgagt attgcggtca aggccaatgc
     3061 caggactgcg gaggctgccc agaaaggtat tttacctgcg ctcagaattg ctttttcaag
     3121 cgggtctgtt atgggcatga cagtagtggg gcttgtgctc ttgggaatag gtgctatcct
     3181 gggccttctt cttttcatgc cagatgctga gcaaatactt gctcaaacga tattcccaat
     3241 agtatcgggg ttttcgcttg gagcctcgat gatggcgctt tttgcacgtg ttggcggcgg
     3301 catttacact aaagcagcgg atgttggtgc cgacctggta ggaaaggtcg aagtcggtat
     3361 acccgaagat gatcacagaa atcctgctgt cattgcagat aatgtcggtg ataatgtggg
     3421 cgatgttgct ggtatgggag cagatctttt cgaatcctat gccggatcta tagttggctg
     3481 catggtgctt ggaattgcaa tttccgccag cccggaactt caacagagat tagctatatt
     3541 gccactcatc gccgcgggaa caggagtggt ggcttcgctt attggaacgc ttttcgttca
     3601 cctaagccga gccaaacgcc ctcagaatgc gcttaatgcg ggctctatag gagcatctat
     3661 aatagccata gtccttttaa taccagtcgt tcgcttcatt atcggagatg gccgtttttc
     3721 gattggatct agagaagcgg ggtggctagg agtctatctt gcatgcataa caggcttggt
     3781 ggcgggtacg gtaattggtc taataaccga atactacacg ggctcgaatt ctccgccggt
     3841 tagaaagata gtaaaatcct gtaccaccgg agccgcaacc acaatcatct ctggcctggg
     3901 tgtcggtatg atttcaacgg tattccctat cctggtaatt ggactggcta tcgggctctc
     3961 gaaccggctt gcagggctct atggcatagg gatcgctggc ctcggaatgc tgctcactct
     4021 cggaatacaa ctctctgtcg atgcttacgg tcctgttgcg gataatgcgg gaggtcttgc
     4081 ggagatggca gaaatggagc cggaagtacg aagaataacc gattcgctag acgccgttgg
     4141 taatacaacg gcagccattg gcaagggctt tgccatagga tccgcgcttc tcacttctct
     4201 catcctaatt gttgccttct tgagtgccgc agaattgccc gtagaaatga tgaaccttgc
     4261 caaccccagt gtgctcattg gtcttttcat tggcggaatg attcccttct ttttcagctc
     4321 gctagccatg gatgccatct atcgagccgc attctcgatg atcgaagagg tgagaaggca
     4381 gttccgcgca aaaccgggta ttctcgaagg aacagatgaa cctgattacc gaacctgtgt
     4441 cgacatctct acacagacag cactaaagag catgatattg ccaggtattt cggccatagt
     4501 cgcgcccgtc ttggcaggct tcatcggtgg gcctgccgtg ctcaccggat tattggtcgg
     4561 cgctacggtc tccggaggtc ttcttgccat atttatgtcg aattctgggg gagcctggga
     4621 caatgccaag aaattcatag aatcgcgtag cgaggaaggc aagggctcta gctctcaccg
     4681 cgcagccgta gtgggagata cagtaggcga tcccttcaag gatacggccg gtccctctct
     4741 aaatattctg ataaaattga tggcggtgat ttcgcttgta cttactcctg ttctaaaagc
     4801 agtatggggt tgatgagtct gcgatcgatg ttttgaccta ttgcagaaat ttctttaaat
     4861 atgtaagaat tgcgataata tatttaggct gtttggaacc ttaggaggtt caccctatgg
     4921 attatgaaaa acatctatat atgattgtcc atccgaatca ctcgctcata gcttcgcagc
     4981 tcgagccaga ccgttttata aaacactatg ttcagggttc cacgcgatac ttcgaaggca
     5041 ggcttatctt tgtagaagtc gatcctgaat ttagaaaccc ctatttcgac atagataccg
     5101 cttattcgga actaaaacct catgaagacg gtaggccaaa agccacaaaa ttcataaaga
     5161 gctatcgggt tttagagcat atggattacg acgcatttgg aaagctttat atcagcaatt
     5221 cgctgggaga ttatgtggaa ctagaatcca aatctcacga accttcgaca gatgcagaag
     5281 aattccgtat catgctagaa gtcaatccag tgcgcttcat tgtgctaaca aaactcaact
     5341 atcatgactt tgcccaattc ataaccgacc cgcgcaacac taaaggtgca ccaaagactg
     5401 ctttcacact cctcgagttc gatagtcaac aattcatgaa agaatatgag gaaaatcctc
     5461 ttatccgctg ctatgtacct ggcatacacc ccgcaaggct cgccgcagcc atagaggaag
     5521 tccgaaacac gccaggcaag gttgtcaagg gcatttccct ggattgcccg atcgaccata
     5581 tctcatataa atatctcaag gaaggattcg tcttcgctga gcagaataga ccatgcaaat
     5641 tctatcccta tctcgacctt ggcactattg aacgaaaatt ctatagattc tggaagagta
     5701 tgtgatttag agtacctatt tcgaagcggc gagagctttc tttttctcga ttcgctctcc
     5761 aaatcttgca atatctataa tggaacgaat atgttccgcc tcgccgactt tttctatttc
     5821 atcccaggcc tcgacctcga aggttagcat gcgcccttcg attttcgcaa gcttagatcg
     5881 aatgcgtact ctcattccct ttggagtcgg agcaaggtgt ttgacggcaa taaaggcgcc
     5941 caccgtggct ttgcctggtt caagtagatg atccaccgca agtcgagaag tctcttccat
     6001 gtgcaacacc atcatcggtg tcgcatagac agggacgcct cctgatccca gatgcgaagc
     6061 aatatatttt tcttccacga ttatttcctt ttcggcaata atcccttcct tcaattccat
     6121 ttctgctctc ctcgctatta gctctctatt tgttcttttc atccatatat tcattgcata
     6181 ctacgctatt gcgcattgga tcttcttcat aaaatactcg atctataaaa gaatcgacag
     6241 ccgccaacca tcgagcctta tcggtcaaaa ttccaaccgc gtgccctgcg ccaggaatta
     6301 gaactaggtc cgtcaatcca atgcgattcg acatccgagc gcttgccatt tgcacactca
     6361 ttatggttgg tacatagcta tcctccattc cgtgagcaaa gataattggc atatcggtga
     6421 gcattactgc ctttgaaggg cttgcctctt ttagatcgaa gcctcgcaga agacggacaa
     6481 aaaatctggt catatgggca ctaggccatg caatgaagtt gggaatgtgg atttccctat
     6541 actgttcgag gagctcctcc caggccgaag aaaatgaaca gtctgctaca ataaaatcga
     6601 tttttctgga ggatgcttct tttgcaagag gtgcatattg aagggctgaa gccgccccaa
     6661 gcgattcacc aaaaagacca tagagccgag catcggaaaa tagcgaatgc aacgcttcaa
     6721 ctccagcttt aatatcatat ttttcataaa aaccatagga tgggtagaaa cgccttggcg
     6781 caatactggc tccatggccg gcaagatcga aagccgcaac attccagccg cgctctataa
     6841 atggtcgcat gtattttacc attccatagc gcgaccaagt tatgccatgg acaaagagcg
     6901 ctgccggtgc attctttttg ccctgaagat actggccttt cagggcatat ccatagggcg
     6961 aggcaaagga ccattcctgc caatcgagat ttagaagatt ctcgtcgaac tcgccgcgct
     7021 caagagaggc ttgccttgta aattcaagac tcttgcattt tggcctaaac ataagtgcat
     7081 aagacaccgc tgtaagtgtg cataccagac cggcataaac caagattatc agccacatac
     7141 aagcattcta gcgcattttg gattggctgg gcagggctgc aaattaccac gcgcgtctaa
     7201 atccaagcct atatctgcgt caagacctgc tgccatacta ttatgaacca caatttcttt
     7261 gctcattgcc cttttttttc tgtttcgcta cactgtttca atacttcata cttaagagaa
     7321 ataggttcgg agaattatca tgcaaaaaaa gcgcattctt acaggcgata gacccactgg
     7381 aaaattgcat ctcggtcatt atgtcggttc actggcaaat cgtgtgcgcc tgcaggacga
     7441 atatgaatgc ttcttcataa tcgcggatct acacacactc actaccagac cggaaaagga
     7501 aaatatagaa cgccttcccg caaatatata ctctgcggtc ctggactatt tatcggttgg
     7561 catagatcca aataaatcgg taatctattt gcagtcagcg gttccagaag tgaccgagct
     7621 ctcgctcttc ttcggtaatc taataactgt acccagactc tctcgcgtac caagtctcaa
     7681 ggaaatggca cagaatgcgc atatcgagga aatgcctttt ggccttttgg gatatccggt
     7741 gctgcaggca gccgatattc tgctccctcg cgcacatctc gttcccgtgg gcaaagacaa
     7801 cgaagctcat gtcgaagtta cgagagaaat tgcaagacgt tttaactatc tatacggtga
     7861 gacctttcca gtgccggaag tgatggtctc ggaatacggc tcgctcgttg gtaccgatgg
     7921 aaataccaag atgtccaaaa gcttgaacaa tgccatcttt ctttccgatg atgcaaaaac
     7981 cgtgaacaaa cgtgtcatgt cgatgtatac cgatccaaaa cgtataagag cggatattcc
     8041 aggcaccgtc gagggaaatc ccgtcttcat atatcacgat gcttttaatc aagataaagc
     8101 cgaagtcgaa gagctcaaag cgcgctatag ggctggaacc attggcgatg tcgaagtaaa
     8161 agaaaggcta gcccgagtcc tgaatgattt tttgggtccc atacgcgaac gtcgagctta
     8221 ctacgaatca caaaaaggcc ttgtcgagga aataatatac gaaggcactc ttcgtatgcg
     8281 acaagaggca agaatcacta tttccatggc aaaaaaagcg atgggcatga gctcaatctg
     8341 gaatcgtatt tccagaaagg cagaagagcg gaaaaaggac gcataaagag cctagagtat
     8401 gctgctcatc tttctccata gaactcgggg ctgatcgacc agactcgaga gcgatttctg
     8461 cccgcttctt ttgccgcata tagagccata tcagccttcc ttatgtactc atgcagaatg
     8521 tcaagtgtgc cgggaatggc tgcatagatt cctatgctta ctgttggcat ttttttattt
     8581 tcttccattt tgatttgagc aattttgttg cgaagcctct ccgcaacaat caaggcttcg
     8641 cgcgtgccta gatcaggaag gagcacaaca aattcctccc ctccatagcg aattgcatag
     8701 tcccgtgtac gaatctgccc acgaatagtc atagctatcg tctgaagaac cacatcacca
     8761 taagcgtgtc cgtatgtgtc gtttatctgt ttgaaatgat cgatgtccag gataagtacg
     8821 ccgattgagt gctcctcttc tatggccttt tgaaaaaggc tgattccatg agtctctaaa
     8881 ccgtaacggt tagctagccc tgtcagcttg tcggtcatgg cctgtcttgt cacgagctga
     8941 tgcctgcgcg cgtgctctag agcaatggca atgtattctg atagctcgct tgcaattctg
     9001 acatgctgat cgttgtaggc gttcaattcc ttgttatcta gagtcagcaa tccaatcacc
     9061 ttcttttcat atataagagg aataccgagc caagacatag tggcgaaatc ttctgtagca
     9121 ttgataaaac ctgggaatga ctgcctaaca tcgttgcaga tgacaatcct ccgctcttca
     9181 atggcgcgca cagccggatt atcaacccca tgagcaggaa aaacaaggcc actgagatcg
     9241 ctttcgttgt agccataacc tgcgacgaca accaatcttc catcttcgag ggattccgcg
     9301 caggctctat caaagggaat aatgcggcgc agttggctag tggttctcga aaatgtctca
     9361 ccaaaatcga ggctcttatt gatggcggta aaaagctctc gcatggtatc gatctcgaca
     9421 agacgctctc gtatttcttc ctgagaattg atgagatcgt cgagatctac gacatgaccc
     9481 actattattc ttggcctttt attgggattc cgcaacagaa cttttatcgt aaagcgaagc
     9541 catctatagg tcgatttatc cttcgaagcg agtcgaaaag catcgatgaa agtctctgtt
     9601 tgacctgacc agatacgctg tagttgtaga ttcgccctct ctctatcgga ctcatggaca
     9661 ggaaagtcct ctatgacctt cagcaagtcc gagtcttcat atccaaaaga tcgaagagtt
     9721 gtacaggatt gcgcggtgtg agatagaaag tcaatataaa agatatcggt ttccttaaag
     9781 aaatccgaaa gtgaagaatc taggaaaaaa tcccttaaga tagaagcttc gagtttcact
     9841 atactataaa attatatgtt atgatgatga ttctgtatat atgaagaaac cactcaatga
     9901 gatatcaaat tcggctttct atgcacggtt taatggctgg tttgaaacaa gttcagcgat
     9961 aggcctacat ttatccaata aaaggaatat ctccagtgtc tccaaaaacc tccgatttct
    10021 tcagcgtggc cttacaggaa atcgaggact catagcctct tcatatatgg acgagtggag
    10081 cattttgcag ccttatttgc tatactattg gcctatctcc ttttacgaag tatcggcaat
    10141 actctacgag ctaaaaacga gagcggtgct tccagatatt aattccgtat tggatttagg
    10201 gagcggaccc gggccggctt ctttcgccgc tcagacgttt ggagccatgc gggctagtct
    10261 tgtggataaa agcaagcgtg cactggcgat ggcctccgcc atagctgcca aaggaaggcg
    10321 taaaaatctt aaccttgaga tcatagaata ttgtgctagc atcgaggact tctgcgctcc
    10381 aaagggcgaa tcctatgatc tgatcatcgc ggctaacagc ctcaatgagt tatgggccga
    10441 ttcgccagat ggaattttaa ggcgacggga tcttgtattg cggttcttgc ccaatttgca
    10501 gcctgaggga atcctcataa tagtggagcc ttccgctcac tatacgagca ttccgctcct
    10561 tgagttgcgc g
//
LOCUS       DAOMZL010000057        10446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_6007_length_10446_cov_16.458570, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000057 DAOMZL010000000
VERSION     DAOMZL010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10446
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6007_length_10446_cov_16.458570"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PLQ30_05725"
     CDS             <1..450
                     /locus_tag="PLQ30_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPW01980.1"
                     /translation="VKIAYGDDLRDKSVSETEGLLRSYPNLKVIIAPTTVGIAAAGKV
                     LTDKGLKGKVYLTGLGLPSEMAEYINNGVCPYMFLWNPIDVGYLGSYVATGLVSGKIT
                     GKVGEKFSAGRLGNYTITQAPDGGTEVLLGPPFKFDKSNIEEWKKVY"
     gene            531..845
                     /gene="rhaM"
                     /locus_tag="PLQ30_05730"
     CDS             531..845
                     /gene="rhaM"
                     /locus_tag="PLQ30_05730"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HPW01981.1"
                     /translation="MKRYAFVMQLKPGNEAEYKRRHDEIWPELKCELRKAGISNYSIY
                     LDPETLRLFAIQSLAEDETSDSLSELPIMRKWWDSMKDLMDTNADNSPKIWSLKEMFH
                     MD"
     gene            864..2051
                     /locus_tag="PLQ30_05735"
     CDS             864..2051
                     /locus_tag="PLQ30_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPW01982.1"
                     /translation="MNLDSIIRLSRRYGADSRYVLAGGGNTSIKQDNVMAVKASGVSL
                     GTIDERGFVLLSLPKLINILEAPLSSDATDPEEIVLERLLASRLPGQTMRPSVETLLH
                     ALFPFRLVVHLHPALVNGLMCSVEALKRVPELFGEEAEVLPYTTPGYTLADRVRTLFR
                     TRKAMGRCPIKILFLRNHGVFVAADNADEIDDLYRHIFDTLEKLVRPLPLDDDSHSVA
                     SKVKNGLPSEDIRVMIEAISSEFNVRFSFTLNSLLGYFLGSPEAFEPLSQPFTPDHIV
                     YAGAWPLFLSQSAASDLKQIKSAIHEYIANHGELPKILAIQRTGIFGLGKDDNAAERA
                     CLLFQDAAKIAWYAQSFGGVHPMESEDADFIRGWEVEKFRSGVAANGLTSPSSESAPI
                     LKK"
     gene            2080..3336
                     /locus_tag="PLQ30_05740"
     CDS             2080..3336
                     /locus_tag="PLQ30_05740"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HPW01983.1"
                     /translation="MPRTEQEYKAAREIYAELAIDTDQAIETLASIPLSIHCWQGDDV
                     IGFDGATSLSGGILSTGSYPGRARNAEELRSDAAFAFSLIPGKKRFNLHAMYAETGGA
                     KVDRCDLEPSYYEGWIEWAKQQHIGLDFNPTFFSHPLAESGWTLAHADNNIRDFWIRH
                     GKASRRIAQSIADALKDHVVNNLWVPDGYKDMPADRLGPRLRLKESLDAIYSEKLPND
                     RVLDSVESKLFGIGSEAYVAGSHEFYLGYASQKAIGLCYDMGHFHPTENVADKISSTL
                     LYVPYLILHLSRGLRWDSDHIVIWNDSLTDVCREIVRMHVWDRVHLALDYFDSSVNRI
                     SAWINGARSTQRALLYAFLEPLEPMRAAEEQADFGTRLSYIEESGSLPFGAIWHHYCE
                     TQNVPYGLGWLEDVKDYEKRVLAKRM"
     gene            3446..4375
                     /locus_tag="PLQ30_05745"
     CDS             3446..4375
                     /locus_tag="PLQ30_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPW01984.1"
                     /translation="MADQQNIDIKDKRYSIYNSEIPMPNPSMTTTTDNFDIEKEIDGN
                     RYAEIVTQSKKPFFAILRAAIKEDFRFMLPRILMRTAKTMRRIPRTFVIKTQEELDQY
                     VFETIFLWSRSLIKITKMEIEVRGADKILPDRTYLFASNHRSPADIPVLFASIPQPAA
                     FVANDLFGRIPALSYWMRASGSVFVNQGLPQSEVRALKAMEKRLKKGRSLILFPEGHM
                     HQGNGIDEFNRGGLFAAVRCAVPIVPICIYGTEKVMRPGSFHINPYRHVVVEFGEPIE
                     TKNLCRADKKGIHLAVHDIIKNMRTALEQEFSS"
     gene            complement(4395..5078)
                     /locus_tag="PLQ30_05750"
     CDS             complement(4395..5078)
                     /locus_tag="PLQ30_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01985.1"
                     /translation="MNETLIAITQWIDNLPSGIGAILILLVGWAIAKLLKLLVPIILA
                     WLKFDVLAEKIGIKEFLRKGNIRYSASKLIGVFLYWVVMLFALAEAASVLDKRAAETI
                     SSWLYGAIPSILATIITATIGIFIVNFLSNFVMTIMSNAGLSSAALVKSLIRVVGYII
                     VIIMVVEQLGFESIMNVLLLLLIGSAALGVAIAIGLGCKDIARQYVDSFLRSLRERER
                     ASHGSDLEG"
     gene            complement(5106..6248)
                     /locus_tag="PLQ30_05755"
     CDS             complement(5106..6248)
                     /locus_tag="PLQ30_05755"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HPW01986.1"
                     /translation="MKNTDWEGIVTHLDHIAAKGKKMVWCVVGAGNGGIAMAGHLGIM
                     GFPVQMYNRTDEHLNAIRWYGGIDIEGMVTGFGPVRLASANMGDALEGSDVIMIVTPS
                     TAHYNLATLMAPFLKDGQIVVLNPGRTGGVLEFRAELERQKCEASVVLVEAQTFVYAS
                     RMITRNRAHIFSIKNGVPVSALPSFLTPAVLKVIDVAFPQFIAGSNVLATSIENIGAV
                     FHPALTLLNAGWIESTGGDFEYYLQGISPSVAMVLQAIDDERLALARALGIRTVSARE
                     WLYLTYDSVGDDLYTAIRSTLAYAGIKAPRSIEHRYIWEDVPMSLVPMASMAAMYGIE
                     TPAINLIIDLANLMLSQDYRANGRTVHSLGIEGMSVEELHRLVTEG"
     gene            complement(6235..8193)
                     /locus_tag="PLQ30_05760"
     CDS             complement(6235..8193)
                     /locus_tag="PLQ30_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01987.1"
                     /translation="MYRVRQTDVVGIVKPAIDAHTLGILSFAQILRDCGIRVEIAGDD
                     VNKEIDRITNEGQLDFLIRWIRDRHIDILGFSYRLDPERGAEIFARLVERLRRLGLMA
                     REGGPLVALWFAGLPPACRRAAERVPMVDATFQGDESYEEVLEKLGLPAHLVPEQASV
                     ELAYDKARLELGKEIVKNRKYLEIKPIDRSGSKRFGLRGERVLDRVHHGIEQHLPPLM
                     RAHVGPYAEDREQAVALFLDWTKQLAKTRMLDILSIGSSQLTQSHFGEDWTGLSNGGG
                     VPINNPAEYREVWEAARPMLVRTYAGTKHVPEMAKMHEEALDICWHALSFWWFSLLDG
                     RGENTVLQNLEEHFEALRYIASTDKAMEPNVPHQFAFRGCDDVGYIVSGYVAAKAAKA
                     QGIRTLILQIMLNTPKYTWGIQDIAKARALRTLVGELEGPDFAVILQPRGGLDYFSSD
                     LEKARAQLAAVTMLMDDIEPDNPASPQIIHVVSYSEGVRLADPEVVEESVQITRYALK
                     EYRRLKKKGDMPEYGKSAMVEARTRSLLKEAKSMIKFIEATIPNTYSPRGFYSMLRSG
                     VFPLPWLIACKDEFEAAVNQPVQFLHGGVHVVDREGKPLSVSQRLFGIARNLEDIGFA
                     PSITHSADSSWLASGSGGGQKEQYGEKH"
     assembly_gap    8372..8471
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8493..9539)
                     /locus_tag="PLQ30_05765"
     CDS             complement(8493..9539)
                     /locus_tag="PLQ30_05765"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01988.1"
                     /translation="MRLLVIMKRNLDQSLAEQRNARYLHMIEIYKHAGFEPYLSYAES
                     IDDIKLSLNNHLPDLVLCGIDHLPENGCNISHNVHAWFEEHNVNYIGSDYKVIELALS
                     KGLLKQKWQTYGIRTPVFGLIREEPEECNEDIESLLSLNAFPYILKPENLGNSRGIDE
                     NSIVWNEAELRKALDKMRLQFGGVILAEHYLGASADFREITCAMIEGSSGMLFMPAEV
                     SLVEPKRFHIITTRDKDKKGTIANPLDADTAASFLPFAERVFAIAGVRDYSRGDFILA
                     DGEFWAIEINGQPMIPDQWFGSAALFAGLSEEKYLVGIVASGYRRLKAEGRLAQVFPK
                     EAQALLKGTKLEVL"
     gene            9724..10374
                     /locus_tag="PLQ30_05770"
     CDS             9724..10374
                     /locus_tag="PLQ30_05770"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPW01989.1"
                     /translation="MKADNERQQERDFMVRFQIAARGIRDKRVLDAMRRVPRHLFVPS
                     SYRVEAYEDYPLPIGSGQTISQPFIVAFMAEALLLKGDERILEIGTGSGYQTAILSLL
                     AKEVYSIERIDALLDRARTILESLEITNVELRLADGFYGWEEKAPFDAIILSAAPFDI
                     PSLLLQQLADGGRLVGPVGPLGAQTLVRVTKHGQHFETETLLDVAFVPMRSGLSKV"
BASE COUNT         2794 a         2592 c         2301 g         2659 t
ORIGIN      
        1 gtcaagatcg cctatggtga tgatctgcgc gataaatccg tctctgaaac cgaaggactt
       61 ctgcgatcct atccaaactt aaaggtgata attgccccaa ctacagtggg catcgcggct
      121 gccggaaaag tgctcacaga caaagggctc aagggcaaag tctatctaac cggtcttgga
      181 ttgccttctg agatggccga atacatcaat aatggagtct gtccttatat gttcctatgg
      241 aatccaatcg acgtgggtta tcttggatca tacgtagcga ccggccttgt gagtggcaag
      301 attactggca aggttggaga gaagtttagc gctggacgtc tgggaaacta caccattact
      361 caagccccag atggtggtac cgaagtattg ctcggacctc cgttcaaatt cgacaagagc
      421 aacatcgaag agtggaaaaa agtgtactga gtgccttttg cgggcttgcc ggcatacgca
      481 agcccgcaat atctatttcc attgtgccct attgatttaa ggataaaaaa atgaaacgct
      541 atgcatttgt catgcagctc aaacctggaa atgaagcgga atataagcgc cgacatgatg
      601 aaatatggcc cgaactaaag tgcgagctgc ggaaagcagg catttcgaat tattcgatat
      661 acttagatcc tgagacatta aggctttttg caattcaaag cctagccgaa gatgagacga
      721 gcgactcctt gtccgaatta ccaataatgc gcaagtggtg ggactcgatg aaagacctca
      781 tggatacgaa tgcggataat tcgccaaaaa tatggtcgct caaagaaatg ttccatatgg
      841 actgaacgca tgggggtttg tgcatgaatc tcgattctat tataaggcta tctcgccgtt
      901 acggcgcgga ctcccggtat gtgttggctg gtggaggaaa tacctctata aagcaagata
      961 atgtaatggc ggtaaaggct tcgggagtct cattggggac aatcgacgag agaggctttg
     1021 tattgttatc gcttcctaag ctcataaata ttctcgaagc tccgctttct tccgacgcta
     1081 ccgatcctga agaaatcgtg ctcgaaaggc ttcttgcctc aagactgccg ggacagacga
     1141 tgcggccgag tgttgaaaca ctgctgcatg ccttattccc atttaggtta gttgtgcatc
     1201 tgcatccggc gctggtaaat ggtctaatgt gctcggtcga agctctcaag agagtacctg
     1261 agctatttgg agaagaagcc gaagtactac cttatacaac gcccggatac accctggcag
     1321 atagagttcg cacattgttt agaacccgta aagctatggg cagatgccca attaagattc
     1381 ttttcttgcg gaatcatgga gtattcgtcg ccgcggataa tgccgatgaa atagatgatc
     1441 tatataggca tatttttgat actctagaaa aattggtgag accgttgcct ttggatgatg
     1501 attctcattc tgtggcgagc aaagtcaaga atgggcttcc atcggaggat ataagggtga
     1561 tgatagaagc gatatcctcg gaatttaacg taagattttc ctttacgtta aattcgcttt
     1621 tggggtattt tttgggttca cccgaagctt ttgaaccact tagccaacca ttcacgcccg
     1681 accacattgt ctatgccgga gcctggcctc tttttctcag ccaatcagca gccagcgatc
     1741 tcaaacaaat caaatcggca atccacgaat acatcgccaa ccatggcgaa ttaccaaaaa
     1801 tcctcgccat ccaaagaacc ggcatattcg gcctaggaaa agacgacaat gcagccgagc
     1861 gggcctgcct gctcttccag gatgcggcaa aaatcgcctg gtacgctcag tcatttggag
     1921 gcgttcatcc gatggaatca gaagatgcag attttattcg aggttgggaa gtagaaaaat
     1981 tccggtcagg agtagctgcc aatgggctga cctctccttc ttcggaaagt gcccctatac
     2041 taaagaaatg atctctggct atgaaggaag gagaacccaa tgcctagaac agagcaagaa
     2101 tacaaagcag cgcgagagat ttatgctgaa cttgccatcg ataccgatca ggccatagag
     2161 accctcgcct ctataccact ttccatccat tgctggcagg gagatgatgt aataggcttc
     2221 gacggtgcaa cttcactctc tggcggcata ctctccacag gaagctatcc tgggcgggcg
     2281 cgcaatgccg aagagctgcg ctcagacgcc gccttcgcct tcagcctgat ccccggcaag
     2341 aaacgcttta acctacacgc tatgtatgcg gagacaggcg gcgctaaagt agatcgttgc
     2401 gatcttgaac ctagctacta tgaaggttgg atagaatggg ccaaacaaca gcatataggc
     2461 cttgacttca atccgacatt cttttctcat cctctagcag aaagcggctg gactctagct
     2521 catgccgata ataatattcg agatttctgg attcggcatg gcaaggcgag tagacgcata
     2581 gcccagtcga tcgccgatgc gctcaaggat catgtcgtca acaatctctg ggtacctgat
     2641 gggtacaaag atatgccggc cgaccgcctt ggtccgcgtt taagactcaa agaatctctc
     2701 gatgccatat attccgaaaa actgccaaac gaccgagtgc tagacagcgt cgaatccaag
     2761 ctcttcggta taggatcaga agcctatgtg gcaggttctc acgagttcta tctaggttat
     2821 gcatctcaga aagccattgg tctatgctac gatatgggcc acttccaccc caccgaaaac
     2881 gtagcggaca aaatttcttc gactcttctc tatgtaccat atctgatcct ccacttgagc
     2941 agaggattgc gctgggactc agatcacata gtcatctgga atgattcgct aacagatgtc
     3001 tgcagagaga ttgtcagaat gcatgtctgg gatagagtcc atcttgctct cgattacttc
     3061 gattcgagcg taaatcgtat ttcagcatgg atcaatggtg cacgcagcac ccaaagagcg
     3121 cttctctatg cttttctcga gcccttagaa cctatgcggg ccgcagaaga acaagctgat
     3181 ttcggaacaa ggctgtcata tatcgaagaa tccggctctc taccttttgg cgccatatgg
     3241 caccattatt gcgagacaca aaatgtccct tatggccttg gttggctcga agacgtaaag
     3301 gattatgaga agagagtctt agcaaagaga atgtgatctt caagaaattg atagccgtaa
     3361 atcgcaaagc ctatacagat attttgattc ttgactaatc gtgtcaaaaa tatagatact
     3421 attattaggg gcattgagcg aacgcatggc agaccagcag aacatagata tcaaagataa
     3481 aaggtattca atttacaatt ctgaaatacc tatgcctaat ccctccatga ctactacaac
     3541 cgacaatttt gatatcgaaa aagaaataga tggaaaccga tatgcggaaa ttgttaccca
     3601 gagcaagaag ccgttttttg ccattctgcg cgctgctatc aaagaagatt tcagatttat
     3661 gctgccccgc attctcatgc gaacagcaaa aaccatgcgt cgaattccgc gcacttttgt
     3721 aatcaaaacc caagaagaac tcgaccagta tgtgtttgag accatctttt tatggtcgcg
     3781 cagcttaata aagatcacaa aaatggaaat cgaggtaagg ggcgcggaca aaatccttcc
     3841 agacaggact tatctttttg cttctaatca ccgatcacct gccgacatac ccgtgctctt
     3901 cgcatcgatc ccccaacctg ccgcctttgt ggctaatgac ttatttggaa gaatacctgc
     3961 tctatcttat tggatgcgcg caagcggctc tgtctttgtc aatcaaggcc ttccgcaatc
     4021 agaagtgcgt gccctgaaag ccatggaaaa gcgattaaag aaaggccgaa gccttatatt
     4081 attccctgaa ggacatatgc accagggcaa tggaatagat gaattcaacc ggggtggatt
     4141 atttgcggcg gttcgctgtg ctgtgcccat tgtgcctata tgtatatatg gaaccgaaaa
     4201 agtcatgaga ccaggcagtt ttcatatcaa tccgtatcgc cacgtggtcg tagaattcgg
     4261 tgagcccata gaaaccaaaa atctttgcag agcagacaag aagggcatcc acctcgcagt
     4321 tcatgacatc ataaaaaaca tgagaacagc tctcgagcag gaattttcat cataaagaaa
     4381 gaatctacag ccggctatcc ctcaagatcg cttccatgcg aagcccgctc acgctcccga
     4441 agagacctga gaaagctatc cacatattgc ctggcaatat ctttgcaacc aagtccaatc
     4501 gctatcgcga ctccaagcgc tgcggaacca attagcaata gaagaaggac attcatgatc
     4561 gactcgaatc caagttgttc aactaccatg attataacaa tgatatagcc caccactctt
     4621 atgaggctct taaccaaggc cgcggaactc agacccgcat tgctcattat tgtcattacg
     4681 aagttcgaaa gaaaatttac gataaagatg ccgattgtcg cagtgattat ggtggccaga
     4741 attgaaggaa tcgcaccgta gagccaggaa gaaattgtct ccgcagccct tttgtcaagc
     4801 actgacgcag cttctgccaa tgcaaaaagc attacaaccc aatacagaaa gacgccaatg
     4861 agcttagaag ccgaatatcg aatatttcct ttgcgcaaaa attccttgat accgatcttc
     4921 tccgcaagaa catcgaattt aagccaggct aatataattg gcaccaggag cttcagaagc
     4981 ttcgcaattg cccacccaac taacagaata aggatagccc caatccctga tggcaaattg
     5041 tcgatccatt gtgttattgc aattagagtt tcattcatta tatttacctc ccttaagagc
     5101 cccgctcagc cctcggtgac aagacggtgg agttcttcta ccgacatgcc ttcgatacct
     5161 agagaatgta ccgtgcgccc attggctcta tagtcctggc tgagcatcag attagcaaga
     5221 tctatgataa ggtttatcgc cggagtctcg ataccataca ttgcggccat actggccata
     5281 ggtacaaggc tcatgggcac atcttcccat atatagcggt gttcgataga acgcggcgct
     5341 tttatccctg cataagccag ggtagaacga atcgcggtat acagatcatc tcccactgaa
     5401 tcgtaggtta gatatagcca ctctctagcg gagaccgtcc ttatccccag agcccgtgca
     5461 agcgcaagtc tttcatcgtc gattgcttga agcaccattg ccactgatgg agaaatacct
     5521 tgaagataat attcgaaatc tccacctgtg gattcgatcc atccagcatt tagaagcgta
     5581 agcgctggat gaaacacggc tcctatattc tctatgcttg ttgccagaac attgctccct
     5641 gcaatgaact gtggaaaggc cacatcgatc acttttagca cggcgggggt aagaaacgaa
     5701 ggcagagcgc tgactggaac tccattcttg atgctaaaaa tatgggctct atttcgagtg
     5761 atcatccgcg aggcatagac gaatgtctga gcttcgacca ggactacact agcctcacat
     5821 ttctggcgct ctagctctgc cctgaattcc agtacccctc cggtccttcc cggatttaga
     5881 actacaatct gtccgtcttt cagaaaaggt gccatcaagg tggctagatt atagtgagca
     5941 gtcgaaggag tcactatcat tatgacatcc gaaccttcaa gtgcatctcc catattagcg
     6001 cttgccagac gcacaggccc gaagccagtc accattccct cgatatctat gcctccatac
     6061 cagcgaatcg cattgagatg ctcgtctgtg cgattataca tttgaacagg aaaacccatg
     6121 atgccgagat ggcctgccat tgcaataccg ccatttcctg ctcccactac acaccagacc
     6181 atcttcttgc ctttggcggc tatatgatcg aggtgtgtta caatgccttc ccaatcagtg
     6241 tttttcacca tattgctcct tttgcccacc tccactccca ctagccaacc agcttgagtc
     6301 agcactatgt gtaatagaag gcgcaaagcc aatatcttcg agatttcttg ctatcccaaa
     6361 gagtcgctga ctcactgaga gaggcttgcc ttctctatcc actacatgaa cgccaccatg
     6421 caaaaactgc acgggctgat ttacagccgc ttcgaattca tctttgcagg caataagcca
     6481 gggtagagga aaaacgcccg atcttagcat cgaataaaaa ccgcgcggac tatatgtgtt
     6541 tggaatggta gcttcaatga atttgatcat actcttggcc tctttcaata agctccgcgt
     6601 tcgagcctcg accattgcag acttgccata ttctggcata tcacctttct tttttaggcg
     6661 gcgatactcc ttgagcgcat acctggttat ctgcaccgat tcttccacca cttcaggatc
     6721 ggcaagcctt acaccttcgg aatagctcac cacatgaatt atttgaggac ttgccggatt
     6781 atctggctcg atgtcatcca taagcatagt caccgcagcg agctgagccc tggctttctc
     6841 caaatcggac gaaaagtagt ccagaccacc cctgggctgc agaattactg caaaatcagg
     6901 tccttctagc tcccctacaa gggtccttaa ggcacgcgcc ttggcaatat cctggattcc
     6961 ccaggtatac tttggagtat tgagcatgat ttgaaggatc aaagtgcgaa tcccctgagc
     7021 cttcgctgcc ttagccgcaa cataaccaga aacgatataa cccacatcat cgcatccacg
     7081 aaaagcaaac tgatgcggca catttggttc catggcttta tcggtggacg cgatatagcg
     7141 caatgcctcg aaatgctctt cgagattctg caagacggtg ttctccccac gaccatcgag
     7201 cagcgaaaac caccagaaag aaagggcatg ccagcagata tcaagtgctt cttcatgcat
     7261 ttttgccatt tcaggcacat gttttgtgcc tgcataggtt cgaactagca tgggacgcgc
     7321 agcttcccat acttctcgat attctgctgg attgttgatg ggtacgccgc caccattgga
     7381 aaggcctgtc caatcttcgc caaaatggct ctgagttagc tgagaacttc caatagagag
     7441 aatatcgagc atccttgttt tggccagttg ttttgtccag tcgagaaata gggcaactgc
     7501 ttgctcgcga tcctccgcgt aagggcctac atgagcgcgc ataagcggcg gaaggtgctg
     7561 ctctattcca tgatgtacac gatccagcac tcgctcccct cggaggccga atcttttgga
     7621 accagagcga tcgatgggct ttatctccaa atatttcctg ttcttgacaa tttccttgcc
     7681 gagctctagg cgagccttgt cgtaggcgag ctccacgctc gcctgctcgg gcacaagatg
     7741 tgcaggtaag cccaactttt ccagaacctc ctcgtaagac tcgtcgccct ggaacgtagc
     7801 gtcgaccatg ggcacgcgct ccgccgcccg ccggcaggcg ggcggcagac cagcaaacca
     7861 cagagcaaca agcggtccac cctcccttgc cataagcccg aggcgccgaa ggcgctcgac
     7921 aaggcgcgca aaaatctcgg cgccacgctc agggtcgagc cgatatgaaa atcccaaaat
     7981 atcgatatgc ctatcgcgta tccatctgat aagaaaatcc aattgccctt cattagttat
     8041 tctgtcgatc tccttgttta catcatctcc agcaatctcc actcttatgc cacaatctct
     8101 taaaatctgg gcaaacgaca aaattccaag tgtatgtgca tctattgcag gttttacaat
     8161 accaactaca tcagtttgtc ttactctgta catttcgaaa cctccttagc acagcattcc
     8221 ctgggctctt agaccgtcat ttcctttttg ctccgccagt tgaaagatca aggcctagta
     8281 ttcggttctt tccatccatg agccccttcc tcctctgtca tttcctctgt catctcctct
     8341 gtcaaaagag ggctctacaa gacctcgagt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nctcctctgt caaaagaggg ctctacaaga cctcgagttt tgtcccttta
     8521 agcagagctt gcgcctcctt tggaaaaact tgcgcaagcc ggccttctgc cttgagtcgc
     8581 cgatagccgg atgcaacaat tcccactaga tatttctctt cggataatcc agcaaacaga
     8641 gccgcactac caaaccattg atctggtatc atcggctgac cgtttatttc tattgcccaa
     8701 aattcaccat cagccaagat aaaatcccca cgcgagtagt cgcgtacgcc agcaatagca
     8761 aaaactctct ccgcaaaagg cagaaaagag gcggcggtat cagcatctag tggatttgcg
     8821 atagttcctt tcttatcctt gtcgcgcgtc gttattatat gaaaacgctt tggctctaca
     8881 agagatacct cagcaggcat aaacaacatg cctgaagatc cctctatcat ggcacaagtg
     8941 atctccctaa aatcggctga agcaccaagg taatgctccg caagaattac tcctccaaat
     9001 tgaagacgca ttttatcaag agccttgcga agttcggctt cattccagac tatgctgttc
     9061 tcatctatgc ctctcgaatt gccaagattt tcaggcttaa gtatgtaggg gaaagcgttt
     9121 agcgataaaa ggctttctat atcctcattg cattcctctg gttcttctct aataagaccg
     9181 aagacaggtg tccttattcc atatgtttgc catttttgtt tgagcaagcc cttggataag
     9241 gccagctcta taaccttata atccgagcca atataattga cgttatgctc ttcgaaccag
     9301 gcatgtacgt tatgcgatat attgcatcca ttttcaggaa gatgatctat tccgcaaagc
     9361 acaagatctg gcaaatgatt attcaggctc aatttgatgt catcaatgct ctctgcatag
     9421 gataaataag gctcaaaacc agcgtgctta taaatttcga tcatgtgtag gtatcttgca
     9481 ttgcgttgct cagcaaggct ctgatcaagg tttctcttca tgattacaag taatcgcatc
     9541 tggtcctccg cgttgatttt ccttttgctc aattcctata tataaatttg taacacaatt
     9601 tggagaaaca tgctaatttt ttttgtcaat attcaaaatt ttctggactt ttgaaatata
     9661 tggacatttc gagacataaa aagccaaatc ctgagttcgc tatcatgata taataggcaa
     9721 aggatgaaag ctgataacga gcgacagcaa gagcgcgatt ttatggttcg cttccagatt
     9781 gcggcacgcg gtataagaga caagcgcgtg ctcgatgcta tgcgccgggt tcctcgtcat
     9841 ctctttgtgc catcgtctta cagggtcgag gcctacgagg attacccttt gccaataggc
     9901 tcgggtcaga ccatttctca gccatttatt gttgctttta tggcagaggc gctcttgctc
     9961 aaaggagacg agcgaattct ggaaataggt acgggctcgg gctatcagac tgctatactc
    10021 tccttgcttg ctaaggaagt ttatagtata gagcgcatcg acgcactttt agatcgcgcc
    10081 cgaactatcc tagaatccct tgaaataaca aacgtcgagc ttcgtctggc agatggtttt
    10141 tatggatggg aagagaaggc tccctttgat gctataatat tgagcgcagc gccctttgat
    10201 attcccagcc ttctgttgca gcagctggcg gatggcggca ggctcgttgg acctgtcggg
    10261 cctcttggag cacagactct cgtaagggtt acgaagcacg gccaacactt cgaaaccgag
    10321 actctcctgg atgttgcctt tgtgcctatg cgctccgggc tgagcaaagt ctaatgcagg
    10381 aatactaaat cattggagag aattatgttt gcacagaatt tgacatttcc tcatttcgaa
    10441 gagcag
//
LOCUS       DAOMZL010000058        10360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_6077_length_10360_cov_19.153421, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000058 DAOMZL010000000
VERSION     DAOMZL010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 10360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10360
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6077_length_10360_cov_19.153421"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..80
                     /locus_tag="PLQ30_05775"
     CDS             <1..80
                     /locus_tag="PLQ30_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPW01990.1"
                     /translation="NANDLEAAKRIVAGTARSMGVDVEL"
     gene            82..768
                     /gene="rplA"
                     /locus_tag="PLQ30_05780"
     CDS             82..768
                     /gene="rplA"
                     /locus_tag="PLQ30_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPW01991.1"
                     /translation="MKRGKKYIEVSKKVDHTQAYEIGDACALVKELKTAKFDETVEVH
                     IRLNLKKSQSVRDTVVLPHQFRAEKRILVFCKPDRVKEALEAGAAYAGSDEYIEKIKG
                     GWLDFDIAVATPDMMKDVGKLGMVLGRRGLMPNPKTGTVTNDLGATLGELRKGRTEYR
                     TDKLGIIHLPVGKVSMEAEKIAENLSTLVEEVNRKRPSDVKGDFVASIFLTPTMGPSV
                     KVVLNKSEKR"
     gene            773..1324
                     /gene="rplJ"
                     /locus_tag="PLQ30_05785"
     CDS             773..1324
                     /gene="rplJ"
                     /locus_tag="PLQ30_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPW01992.1"
                     /translation="MAIKASKIQPRKVKAIGMLKEMISGSSDFIFTEYRGLTVEQITT
                     LRRQLREKGVELHVVKNNFARLAFEELGYSHAVEPVLRGPTAVAFIKTDSNEVAKILL
                     DFAKETPALVVKGAMIDRQFMDSDRVETFSKLPGRNQLIAMLMSAMQAPAQNLVYVIN
                     AIPTKLVRVLKAIEEKKANEGVL"
     gene            1453..1839
                     /gene="rplL"
                     /locus_tag="PLQ30_05790"
     CDS             1453..1839
                     /gene="rplL"
                     /locus_tag="PLQ30_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPW01993.1"
                     /translation="MAALTKEQIIDAIASMTVLEISELVKAMEEKFGVTAAAPVAVAA
                     PAASAAAAPVEEKTEFTVILKGNVPADKKIAIIKEVRSITGLGLKEAKDLVEAGDKAL
                     KENISKEEADKIKKQIEAAGGTVEIK"
     gene            2043..5537
                     /gene="rpoB"
                     /locus_tag="PLQ30_05795"
     CDS             2043..5537
                     /gene="rpoB"
                     /locus_tag="PLQ30_05795"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPW01994.1"
                     /translation="MTYSGQKIKRIYIGKEFHDVMDIPDLIDIQLSSYEHFLQRDKLR
                     KGEPLELIGLEEVFRSTFPIESPSGDLVIEYERYSLDEKAIKYSELECKQKTLTYSVP
                     LKAVINLVFKRTGEIRQKEIYLGDIPLMTDRGTFIINGAERVIVSQIHRSPGVIFSHD
                     KGVFSSRIIPYRGSWLEFEIDQKHELIHAKIDRKRKILGTMFLRAIGFHSREEIIAAF
                     YTTKKAELSEDQNNKDALVGKILAKPVYMEIDNEQKRLFRAGDKIHLHEIDELINLGI
                     DSIEIIDFEDDNSLHNEMILNCFDREDVKLVKETPDQDEPTKEDAISAVYSVLRPGEP
                     ISLENAERDLHNMFFTARQYDLGDVGRYKLNKKFDFDIKTNTLTKEDIVATMKHLINI
                     YYGEANIDDIDHLGNRRVRAVGELLANVMKVAFSRMERTAKDRMTGKDLETAKPQDLI
                     TIKPIVAAIKEFFGTSQLSQFMDQVNPLAELTHKRRLNALGPGGLNRERAGFEVRDVH
                     YTHYGRMCPIETPEGPNIGLIVSLATYTTVNEYGFLEAPYRRVRNGIVTDEIEYLSAI
                     DEDKYYIAQASAHIDKSGKFLDQTVSCRHQGDYTMRSPAEIQFMDVSPKQIISVSAAL
                     IPFLEHDDANRALMGSNMQRQAVPLVFTEPPRIGTGMEWKTAYDSGVLVKAKRSGIVD
                     YVDANRIVIRPEKPESPDDTDVYQLIKYQRTNQDTCFNHRPIVKRGEKVLKGQVVADG
                     PATNQGELALGRNILAGFVPWNGYNYEDAILISERVVKEDLFTSIHIKEFILEVRDTK
                     LGPERITRDIPNTSEKMLDNLDGQGVIRIGAKVKSGDILVGKVTPKSDSDTTPEFKLL
                     NSIFGEKAKDVRDSSLRVPHGIEGTVIDVQRMSRANNDDLQPGVEETVKVLVAVKRKL
                     KEGDKMAGRHGNKGVVARVLPIEDMPYLDNGTPLDICLNPLGVPSRMNIGQIMETELG
                     WAASTLNEWYECHVFQSPSQEQIEVKLREAGLPATSKVMVRDGKTGEFFKNPITVGIV
                     YFLKLHHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRLGEMEVWALEAYGAANTLQ
                     ELLTIKSDDMTGRSKIYEAIVKGEPHTAAGIPEAFNVMVQELRGLALDIKVYDSKGKQ
                     IALTERDEDIIKKQTGAF"
     gene            5673..10022
                     /gene="rpoC"
                     /locus_tag="PLQ30_05800"
     CDS             5673..10022
                     /gene="rpoC"
                     /locus_tag="PLQ30_05800"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPW01995.1"
                     /translation="MRDIQDFDNISIHLASPEMIRAWSYGEVKKPETINYRTLRPERD
                     GLFCERIFGTTKEWECYCGKFKSIRYKGVICDRCGVEVTNSKVRRERMGHIELASPVS
                     HIWFYRSVPSRMGLLLNLPMATLKSVLYYEKYIVIDPGETELKKMQLLTDEEYREAMD
                     RYGGAFTAGMGAEAIRSLLEQIDLDKMSQELRQKMIERGSKSDKRLLKRIELVEAFRS
                     SGNRPEWMILTVIPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKKLLQLNA
                     PDIIIFNEKRMLQEAVDALFDNTRRRRAVKGAGNRPLKSLADMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKMWQCGLPTKMALELFKPFIMKKLVEKDVVYNIKKAKMLVES
                     ETPEVFAVLDEVVKEHPVLLNRAPTLHRLGIQAFEPLLVEGKAIKLHPLVCKAYNADF
                     DGDQMAVHIPLTHAAQAECWTLMLSSRNLLDPASGKTIVYPSQDMVLGINFLSRHRPG
                     AKGEGKRFSSTDELLLACEYGSCEYQALVYVAVRKEPVWNSYEKEAEPSKNHFIKTTG
                     GRILLNEALPKEVPYINYAMTDKDIRMLIEHVYRNTSPFITVNMLDTIKEMGFKYATF
                     FGATIGMDDIVIPKEKAEMIEKANKEVEAIQQQYLAGHITQDERYNRVVEVWSKTNEE
                     LTAVMMKTLEKDRDGFNNIYMMANSGARGSKNQIRQLAGMRGLMAKPSGDIIELPIRS
                     NFREGLSVIEFFISTNGARKGLADTALKTADAGYLTRRLVDIAQDVVINEDDCGTING
                     SIYTALKDGDEIVETLAERIIGRFTLDPIRHPITGQIIVGTNEEITEEISEEIEEAGI
                     GEVSVRTVLTCEAKHGVCRKCYGRNLASGRTVDIGEAVGIIAAQSIGQPGTQLTMRTF
                     HIGGAATKAAEENRISLHYPVYIADIEGSYVKTELGRLLFTRRGYIYVNRVFGVFDFT
                     DEDTLLVKDGQRVLKDDSVIKKADGSYIKSPSISYAKVLGNRLLLISQEQKVEIRNGS
                     EMVVSRGQILLANETIATFDPFNDPIISEHDGIVRYEDIMTGVTLNEEINAETGNVEK
                     IIADAGNERDTKEPRIIITDEAGNDIETYYLPEGAYVNVEDGESVRAGRMLAKILKES
                     AKAMDITGGLPRVGELFEARKPKSPAVLALVSGTVTFGGTLKGKRVVNIRDSFGKTHK
                     HLVPKNRRLLVRNGDKVVAGEMLCDGVKNPHDILAILGEQECQRYLMDEVQQVYRAQG
                     VTINDKHIGVIIRQMMKKVEIVNPGDTVFIYGQQVDKHKFHEENRRVMEEGGQAAVAR
                     PILLGITRASLKIDSFFAAASFQETTRVLTDAAIKGEVDQLRGLKENVIIGHLIPAGT
                     GMKQYRQLRLFDAENDDLNALVEEVLEKRKQERELAPPAILQDRTSEISFEETTIFDN
                     DEGFSEEDQSLEE"
BASE COUNT         2973 a         2401 c         2681 g         2305 t
ORIGIN      
        1 tcaatgcaaa tgatctcgaa gccgcgaaac gcatagttgc ggggaccgca aggtccatgg
       61 gcgtggatgt ggagttgtga tatgaagcgc ggtaagaaat acatcgaagt atccaagaaa
      121 gtggatcata cgcaggccta tgagattggg gatgcatgtg cactcgtaaa agaactcaag
      181 acagccaagt tcgatgaaac ggtggaagtg cacataaggc taaaccttaa gaagagccaa
      241 tctgtgcgcg acacagtcgt tctcccccat caattccgag cagagaagag aatccttgtg
      301 ttttgcaaac ccgatcgcgt caaagaagct ctcgaggcgg gagctgccta tgcagggagc
      361 gatgaataca tagaaaaaat caagggtgga tggctagatt tcgatatcgc cgttgcgaca
      421 cccgatatga tgaaagatgt cgggaaattg ggtatggtgc tcggacgccg cggccttatg
      481 ccgaacccta agacaggtac ggttacaaac gatctgggcg ccacgcttgg cgaactgaga
      541 aaaggccgca ccgaataccg caccgacaag ctcggcatta ttcacctacc tgtcgggaag
      601 gtatccatgg aggctgagaa aattgccgag aatctaagca cgcttgtaga agaagtaaat
      661 cgcaagcgtc cttctgatgt aaagggcgat tttgttgctt cgatattctt gacgccaacc
      721 atggggccaa gcgtcaaagt cgtactgaac aaatcggaga agaggtaaga ctatggcaat
      781 taaagcatcg aaaattcagc caaggaaagt caaagccata ggcatgctca aagaaatgat
      841 ctcgggctca agcgatttta tctttaccga gtacagaggc ctaacggtcg aacagatcac
      901 aaccttacgg aggcaactgc gcgagaaagg tgtagaactc catgttgtca agaacaactt
      961 tgcgcgtttg gcctttgagg agctcggtta ttcgcatgcg gtcgagcctg tgctaagagg
     1021 gcccaccgcg gttgctttta tcaagaccga ctcgaatgaa gttgccaaga ttttgctcga
     1081 ctttgccaag gaaacaccag cactggttgt aaagggtgca atgatcgata gacaattcat
     1141 ggattctgat agggttgaaa cattcagcaa gttgcctggc cgcaatcagc taatagcaat
     1201 gctaatgtct gccatgcaag caccagcgca gaacctcgta tatgtcatca atgcaattcc
     1261 gaccaagttg gttcgagttc tcaaggcgat cgaggaaaag aaggccaatg aaggggttct
     1321 ttgatcgttt attcggcatg ctttttggaa aaagcgagca tccgtcaggc ttcggacgct
     1381 gccgttcctg tcggagttcg atagaaggtc cattcgttgt gatggattac catgagatta
     1441 ggagaagata atatggcagc actcacgaaa gaacaaatca tcgacgcaat tgcttcgatg
     1501 acagtcctcg aaatttccga actggtaaaa gcgatggaag agaagtttgg cgtaaccgcg
     1561 gccgcacccg tggcagttgc cgctcccgct gcttctgctg cagctgctcc tgttgaggag
     1621 aagaccgagt ttacagtgat cctcaagggc aatgtgccag ctgacaagaa aatcgctatt
     1681 attaaggaag tccgttcgat caccggtctt ggcctgaaag aggctaaaga cctggtcgaa
     1741 gctggagaca aggctctcaa ggagaacatc tctaaagaag aagcggacaa gatcaagaag
     1801 cagatcgaag ccgctggtgg aacagtcgag ataaaataag tctgggcttg gagttagagc
     1861 cttagtcgat gaaaagaagg gggattgaga tcccccgttt tcgggaagca ttccagccga
     1921 ttgaatcttg caaaaaagag tgcttcccga tgacggaata aaataaagca ccgatgttcg
     1981 atatatcggc atcggcgcat gaacctttga cccgagcgct aaaggcgcat ggagggtaat
     2041 gaatgacata ttcaggacag aagatcaagc ggatttacat aggaaaggaa ttccacgatg
     2101 taatggatat acccgatctc atcgatattc agctctcatc ttacgagcat tttctccaaa
     2161 gagataaact ccgtaaggga gagcctctcg aattgatcgg ccttgaggag gtattccgca
     2221 gcacttttcc tatagaaagt ccatccggtg atctggtgat cgaatatgaa cgatattctc
     2281 tagatgaaaa agccatcaag tattctgaac tagaatgcaa gcagaagaca ctgacctatt
     2341 ccgtgcctct aaaggcagtc ataaaccttg tgttcaagag gaccggagag ataagacaga
     2401 aagagatcta cttgggcgat atcccgctaa tgaccgaccg cggtacattt atcatcaatg
     2461 gggcggaacg agtcatcgtt tctcagattc atcgttcgcc gggcgttatt tttagtcatg
     2521 acaaaggcgt attttcctca cgcataattc catatagagg aagttggctc gaattcgaga
     2581 ttgaccagaa gcacgagctc attcacgcca agatagatcg caagcgaaag atcctgggaa
     2641 ccatgttctt gcgcgcaatt ggctttcatt cgcgcgaaga gatcatcgcg gcattctata
     2701 caaccaagaa agcggagctc tcggaagatc agaacaataa agatgctctt gttggcaaaa
     2761 tacttgcaaa acccgtatat atggagatcg ataacgagca aaagcgcctt ttccgagccg
     2821 gcgacaagat tcatctgcac gaaatcgacg aactcatcaa cctaggcata gattctatcg
     2881 aaatcataga ttttgaagac gataattcgc tgcacaacga gatgatcttg aactgcttcg
     2941 atagagaaga tgtcaagctt gtaaaggaaa caccggacca ggatgagccg actaaggaag
     3001 acgcgatctc ggcggtttat tctgtgctca gaccaggtga acccatatct ctggagaatg
     3061 ccgagcgcga tctacataac atgtttttta ccgccagaca gtacgatctg ggcgatgttg
     3121 gccgctacaa gctaaacaaa aaattcgatt tcgatatcaa aacgaataca ctcaccaagg
     3181 aagatatcgt cgcaacgatg aaacacctca taaatatcta ttatggggaa gcgaatatcg
     3241 atgatataga tcatctagga aatcgccgcg tacgcgcagt tggcgagctt cttgcaaatg
     3301 tcatgaaggt agcttttagc cgtatggagc gcaccgcaaa ggacaggatg accggcaaag
     3361 acctcgaaac agcaaagcca caagacctca ttaccatcaa gccaatcgta gcggctatca
     3421 aagaattctt tggtacctcg cagctttctc agtttatgga ccaggtcaac ccattggccg
     3481 agctaaccca caagcgcaga ctgaacgccc ttgggcctgg tggactaaac cgcgaacgcg
     3541 cgggcttcga agtgcgcgat gtccactaca cccattatgg acgaatgtgc cccatcgaga
     3601 cgccagaagg acccaacatc gggttaattg tatcgcttgc aacctatacg acggtcaacg
     3661 agtacggatt ccttgaggca ccgtatcgcc gcgtaagaaa tggcattgta accgacgaaa
     3721 ttgagtacct ttccgctatc gacgaagaca aatattatat cgcacaggct tctgctcata
     3781 tcgacaaaag cggtaaattt ctcgaccaaa ccgtatcctg ccgccatcag ggcgactaca
     3841 caatgcgatc tccagcggaa atacaattca tggatgtctc gcccaagcag ataatatcgg
     3901 tatcggcggc tctgattcca ttcctcgagc acgatgatgc taaccgagct cttatgggtt
     3961 cgaatatgca gcgccaggcg gtgcctttgg tttttaccga acctccgcgc atcggcactg
     4021 gtatggaatg gaaaacggct tatgattcgg gtgttttggt caaggccaaa cgctccggaa
     4081 tcgtcgatta tgtggacgct aacagaattg tcattcgacc tgaaaaaccc gaatcgcccg
     4141 atgatacgga tgtatatcag ctcataaaat atcaaaggac caatcaggac acctgtttca
     4201 accacaggcc gattgtaaag cgcggcgaga aggtgctgaa aggccaagtc gttgcggatg
     4261 ggccggctac taaccaggga gagctggcac ttggccgcaa cattcttgct ggattcgtgc
     4321 cctggaatgg ctataactac gaggacgcca tcctgatatc cgaacgtgta gtaaaggaag
     4381 acctctttac ctctatccat ataaaggaat tcattttaga ggttcgcgac acaaagctgg
     4441 gtccagagcg cataacgcgc gacattccaa atacaagcga aaaaatgctg gataatctag
     4501 atggccaagg cgttattagg attggcgcca aagtaaagag cggagacatt ttggtaggaa
     4561 aagtgacccc aaaaagcgac tcggatacaa ctccagaatt caagcttcta aactcgatat
     4621 tcggcgaaaa agccaaagac gtccgcgatt cctcgctccg cgtgccacat ggcatcgagg
     4681 ggacggtcat agacgtacag cgcatgtcac gagcaaacaa cgatgatctc cagccgggcg
     4741 tcgaagagac cgtaaaggtt ctggtagcag taaaacgaaa gctcaaggaa ggcgacaaga
     4801 tggcaggtcg tcatggcaat aagggagtcg ttgcacgcgt tctccctatt gaggacatgc
     4861 cctacctgga taatggtacg ccgcttgaca tctgcctaaa cccgcttggt gtcccgagcc
     4921 gcatgaatat tggccagatc atggagaccg agctcggttg ggcagccagc actctaaatg
     4981 aatggtatga atgccatgta ttccaatcgc cctctcaaga acagatcgaa gtaaagctaa
     5041 gagaagcggg gttgcctgcc acatcgaagg ttatggttcg cgatggcaag acgggagaat
     5101 tcttcaagaa tccgataaca gtcggaatcg tatacttcct gaaattacac cacctcgtag
     5161 atgacaagat gcatgctcga tcgactggcc cttattcgct ggtaactcag cagcccctgg
     5221 gcggcaaagc gcaatttggc ggccagagat tgggcgagat ggaagtgtgg gcactcgagg
     5281 catatggggc agcaaacacc ctgcaagagc ttcttacgat caagtcggac gacatgacag
     5341 gacgctccaa gatctacgag gcaatagtca agggggaacc acatacggca gccggaattc
     5401 ccgaggcctt caatgtcatg gtgcaggagc ttcgaggctt ggcgctggac atcaaggttt
     5461 atgactctaa gggtaaacag atcgctctaa cggagcgcga tgaagatatt atcaagaagc
     5521 agaccggagc attctaggat ggcacatttt gctgttgcac aatccatgtt tgccggactt
     5581 tgcacggtct ttagtgcgcc ttttttgggt agagggcgct attggaacaa tattccgagg
     5641 catgagaaat gcctacaacc cgagggggta tcatgagaga tattcaggat tttgacaata
     5701 tttccattca tctggcaagt ccggagatga ttcgggcctg gtcttatgga gaggtcaaga
     5761 agcctgagac aatcaattac aggacattgc ggcctgagag agatggcctt ttctgcgaac
     5821 gcatctttgg cacgaccaag gaatgggagt gctattgtgg caagtttaag tctatcaggt
     5881 ataagggagt catctgcgac cgctgcggtg tcgaagttac caattctaag gtgcggcggg
     5941 agcgcatggg gcatatagag ctagcgagcc cggtatcgca catttggttt taccgctccg
     6001 tgcccagccg tatgggtctt ttgctcaatc tgccgatggc gacactcaaa tctgtcctct
     6061 attacgaaaa atatatcgta atcgatccag gagagaccga gctcaagaaa atgcagctcc
     6121 tcaccgacga ggaataccgc gaggctatgg acagatatgg gggcgcattt accgctggca
     6181 tgggagccga agccattcgc tctcttcttg agcagataga tctcgacaag atgtctcagg
     6241 aactcagaca gaagatgatc gagcgcggaa gcaagtccga caagcgcctt ttgaagcgta
     6301 tcgagctcgt agaggcattt agatcttccg gaaatcggcc tgaatggatg atcctaaccg
     6361 tgattcccgt aattccgccg gagttaagac ccatggttca gctcgatggt ggtcggttcg
     6421 ccactagcga cctaaacgat ctctatcgtc gagtcataaa ccgcaataat cgcctcaaga
     6481 agcttttgca gctaaacgcg ccggatatca tcatctttaa tgagaagcgc atgctccagg
     6541 aagccgtcga tgcgctattc gacaacactc gtcgccgcag agcggtaaag ggagcgggca
     6601 ataggccgct caaatccctg gcagatatgc taaagggaaa acaggggcgc ttccgtcaga
     6661 acctattggg taagcgagtc gactactctg gccgatcggt aatagttgtt gggccagagc
     6721 tcaagatgtg gcagtgcggt cttcctacca agatggctct cgagctattc aagcctttca
     6781 tcatgaaaaa actggtggag aaggatgtcg tatacaacat aaagaaagcc aaaatgctgg
     6841 tagaatcaga gacgccagaa gtctttgccg tactggacga ggttgtaaag gagcatccgg
     6901 tgctccttaa tcgcgcccca actctgcacc ggcttggcat ccaggctttt gagcctcttc
     6961 tcgtagaggg caaggcgata aagctgcatc cgctggtgtg caaggcctat aatgccgact
     7021 tcgatggcga tcaaatggcg gttcatattc ctctaaccca tgctgcccag gctgaatgct
     7081 ggactcttat gctttcgagt cgcaacctgc ttgatccagc aagcggaaag acgattgtct
     7141 atccttctca agacatggtt ctcggaatca actttctaag ccgccataga ccgggggcaa
     7201 aaggcgaggg caagcgattt tcaagcacag atgagctcct tcttgcctgc gaatatggtt
     7261 catgcgaata tcaggctctt gtctatgtgg cagtacgcaa ggaaccagtg tggaattcct
     7321 atgaaaaaga agcagagcct tccaagaacc acttcataaa gacgacggga ggccgcattc
     7381 ttctaaacga ggctctgcca aaagaggttc cttacataaa ctatgcaatg accgataaag
     7441 acattcgcat gctgatcgag catgtctatc ggaacacgag cccttttatc accgtgaata
     7501 tgctcgacac aatcaaagaa atgggcttta agtatgcgac tttctttggc gctaccatcg
     7561 gcatggatga catcgtcatt ccaaaagaga aggccgaaat gatagagaag gccaataagg
     7621 aagttgaggc aatccagcag cagtatcttg caggccacat aactcaagat gagcgctaca
     7681 accgtgtcgt cgaggtgtgg tcaaagacaa acgaagaact cactgctgtg atgatgaaga
     7741 cgctggaaaa agaccgtgat ggcttcaaca atatatacat gatggctaat tcaggggctc
     7801 gcggaagcaa aaaccagatc cgccagctag cgggaatgcg tgggcttatg gctaagccat
     7861 ccggagatat aatcgagctt cctattcgtt ccaatttccg tgaaggtctt tccgtcatcg
     7921 agttcttcat ctctacaaat ggagcccgta aaggtctagc agacacggct ctcaagaccg
     7981 cggatgcagg ttaccttaca aggcgcctcg tagatatcgc acaggatgta gtaatcaacg
     8041 aggatgactg tggaaccata aatggttcca tctatacggc actcaaagat ggcgacgaaa
     8101 tcgtcgaaac actagccgag cgcataatcg gtcgctttac cctagaccct atccgccatc
     8161 ccataactgg ccagatcata gtgggaacca acgaggagat taccgaggag atctccgagg
     8221 agatagaaga agcaggaatc ggagaagtct cggttcgcac cgttttgact tgcgaggcta
     8281 aacatggcgt atgtcgcaag tgctatggcc gcaaccttgc cagcggcaga accgtagata
     8341 ttggagaagc tgttggaata attgcggcac aatccatagg ccaaccgggc acccagctca
     8401 ccatgcgcac tttccatatc ggtggtgcgg caacaaaagc cgcggaagag aaccgaattt
     8461 cgcttcatta cccagtatac atcgcagata tcgaaggttc ttatgtaaag acggagcttg
     8521 gtcgtctgct attcactcgc cgcggatata tctatgtgaa tcgagtcttt ggcgttttcg
     8581 attttactga tgaagatact ctccttgtca aagacggcca gcgcgtcttg aaagatgatt
     8641 cggttatcaa gaaagccgat ggtagctata taaaatcccc gagcatctct tatgccaagg
     8701 ttctcggcaa cagactcttg ctaatctctc aggagcagaa ggtagagatt cgaaacggtt
     8761 ccgaaatggt ggtatcgcgc ggtcaaatcc ttcttgctaa tgagactata gctaccttcg
     8821 atccctttaa cgacccgata atatccgagc atgatggcat tgttcgctac gaagacatca
     8881 tgaccggcgt tacactgaat gaagagatca acgccgagac cggcaacgtg gaaaagatca
     8941 ttgcagatgc aggcaacgag cgtgatacca aagagcctcg aataataata accgacgagg
     9001 ccggaaacga tatcgagacc tattacctcc ctgaaggcgc ctatgtaaac gtcgaggatg
     9061 gcgagagcgt tcgcgcaggc aggatgctgg caaagattct aaaggaaagt gccaaggcta
     9121 tggatatcac aggcggtcta cctcgggttg gtgagctctt cgaagcgaga aaacccaaat
     9181 ccccagccgt tctcgcattg gtctctggaa ctgttacctt tgggggcaca ctcaaaggca
     9241 agcgcgtagt caatatccgc gattcctttg gaaaaaccca taagcacctc gtgccaaaga
     9301 atcgacgact tttggtgcgc aatggggata aagtggtcgc aggcgagatg ctctgcgatg
     9361 gggtaaagaa cccacatgat atccttgcga ttttgggtga acaggaatgt cagcgctacc
     9421 tcatggatga agtgcagcaa gtctaccgtg ctcaaggcgt aaccatcaac gataaacata
     9481 taggagtcat catccgacaa atgatgaaga aggtcgaaat agtaaatcca ggtgacacgg
     9541 tatttattta tggccagcaa gtggataagc ataaatttca tgaggaaaac agacgggtga
     9601 tggaagaagg cggacaggct gcagtagcgc gcccgattct gcttggtata acgcgtgcat
     9661 ctctaaagat cgattccttc tttgccgcgg cgtctttcca ggagacaaca agagttctga
     9721 cagatgcagc cataaaggga gaggtggatc aactgcgagg gctcaaggag aatgttatca
     9781 tagggcatct tataccagcg ggaaccggaa tgaaacaata ccgtcaactg aggcttttcg
     9841 atgctgaaaa tgatgatctc aacgccttgg tagaggaagt gctcgaaaag cgaaagcagg
     9901 aacgcgaact agctccacct gctatattgc aagatcgtac aagcgagata agctttgagg
     9961 aaacgacgat cttcgacaat gacgaaggat tcagcgaaga ggaccaatcc ttggaggagt
    10021 aagatcccaa gggtttttga gcatgcccaa ttgtataact ttatggtaca ggcattgctc
    10081 tgtatgtgct tgatatggcc actgcgattt aacttcggct agaatatagt ttttaggcga
    10141 tggcttatta tcggccagaa aactttttag gcaattcgag gccttgacat gtgatgggtg
    10201 agaaaggtat tctacaccgc tgctaaaaca gacaccgcag tttttgcaga ccggtaattt
    10261 aaattatatt gaagaaaacc gcgcgcgatg ctgacgcgag cggatgaaag ggagtctaga
    10321 gcttatatgc cgacgattaa ccagctaatc cgaaatggaa
//
LOCUS       DAOMZL010000059        10338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_6094_length_10338_cov_15.390839, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000059 DAOMZL010000000
VERSION     DAOMZL010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 10338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10338
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6094_length_10338_cov_15.390839"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLQ30_05805"
     CDS             <1..560
                     /locus_tag="PLQ30_05805"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HPW01996.1"
                     /translation="IEISTPLVGSIYAGNMAVSALALSQLDIELNTEHMLQGFSSSSM
                     PARFEIVSHSPFVVLDGAHTPESIRTNLSTFLELSKAPRILLFGCAHDKKHDEIAKII
                     SPYFDEIIITRPGTFKQSEPAIVFDSFQKQMSNSIISDRYLIEDTNSAIAKALSDARE
                     KNASLLVTGSFYLCAEFKKSRLCSE"
     gene            complement(532..2214)
                     /locus_tag="PLQ30_05810"
     CDS             complement(532..2214)
                     /locus_tag="PLQ30_05810"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HPW01997.1"
                     /translation="MEFFKAWILPPLVGAIIGYFTNWLAIKMLFRPYKTIKFAGIALP
                     FTPGLLPREKDKLAVSLGETVSKELLTAEVITQRIQSADIKQTILKAIKSALASFFNQ
                     DAGRIFSQIGEPGLEEKKDDEPSLLLLESNGQKITKEVQSGNGTTIDSEVSALQRNII
                     EALQHLVERKSVQSSIEGIFAEFLNRIGKLSFKELVSKEQFIASVLKACSTLQTRVLS
                     ESNHEQSLQSSKSKSSRFNPLLKAVIDLPPDVTIMAFTDALVPQVYTWFLDYIDSFFH
                     SEGFRARLETEAQAFVKRALDRLGPIQRLFISIAGYDAKIAQAMPENIEDLIQTIERI
                     LKDPSTPQSISKTISRAIINWRSKDEAETTKTGPEIYQRADDSIGSKSRVVSEQIVLS
                     LKDSSEDLRLRAEAIYHSLADLEFGEILGIRSNEEAISGFVFSIIVPTMHVSDSSASN
                     TMGSIFIEVLKDVSKGKTFADILKIQDSEIERISVSLTDGLIELIQSRISTLIDAFDI
                     TSMVSERIDSLDMKEVERIVLQVVKKELAWITWLGGILGGMIGFIQSIISTF"
     gene            complement(2217..2438)
                     /locus_tag="PLQ30_05815"
     CDS             complement(2217..2438)
                     /locus_tag="PLQ30_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPW01998.1"
                     /translation="MKKDPTNSSQNLSILRKGTVLRIIESKYSTSESDRGTLWFRIQE
                     AGQTGWVPALEVMTYSSEKQARNAALRME"
     gene            2649..3302
                     /locus_tag="PLQ30_05820"
     CDS             2649..3302
                     /locus_tag="PLQ30_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW01999.1"
                     /translation="MNMTIGKAKAKPFLFLVFMVCKLGATQAIGALPALEFGMGFAYA
                     RTKVESAATGYHHGWNVSADANWLFRISLEDENLIKAKAEKETSSRVNMIFSPGLHAQ
                     FITMSASNWLPDGSRYRAWDSIGLGPELNLGLEFTNSTTMHKNGFLFSMAYLASFSNY
                     TQTTLYSAYNSWFLKVSWNARIDNGAAFFAALPLEYAFRADGNSLLSGLVIGMRYEF"
     gene            3394..4329
                     /locus_tag="PLQ30_05825"
     CDS             3394..4329
                     /locus_tag="PLQ30_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPW02000.1"
                     /translation="MKGAFFLLVLWPLLASCEILAPTNATFEGEKYAVVVGINDYIDN
                     TNNLIRDLDFCAADAEAMKDMLTGAGWQVELITAEANESIKKNATKEKIKTAFQKDKD
                     GLTAFLFYFSGHGDVHEDQAYIVPSDAYRSGNSVEKSSMISATELSMWLKDLNANKIM
                     IFDSCYSGGFVDPGDCIDAAPGNIESDQISTNIEMFFRFGEILAQNSASAARSPSTAP
                     LVISASGSNEASMEDANERPTHGSGHGFFTYYFLQSAEASKDGSMKGDLDGDGILSCL
                     EAYNYAKNALEKDWIASQLPHITGGLRDFALIDLR"
     gene            complement(4371..4859)
                     /locus_tag="PLQ30_05830"
     CDS             complement(4371..4859)
                     /locus_tag="PLQ30_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HPW02001.1"
                     /translation="MRYNLTTAYFLWLISGFGALGFHRFYLGKTGTGLLWMFTGGLGG
                     LGGLYDLITLPRQVREANLAYSAREAIGHENYGQWGEFIPHIRQSETPERVILRLAKA
                     NNGMVTAGEVAIEANIPIEEAQRQLDNLAKKSIAQVRVRSSGVLVYFFPEFSKEDTDF
                     ID"
     gene            4992..6392
                     /gene="asnS"
                     /locus_tag="PLQ30_05835"
     CDS             4992..6392
                     /gene="asnS"
                     /locus_tag="PLQ30_05835"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPW02002.1"
                     /translation="MTLLPTIKQLLSMEAVGQPVIIRGWVRTKRETKQAIFIEVNDGS
                     CLSNIQCVLDSSLEKNQSINETILRLSTGASVEIHGELVPSPAKGQKVEVKCQQIKLI
                     GEAPSERYPLQKKAHSLEFLREIAHLRVRTNTFAAVARVRNRMAFAIHQFFQERGFYY
                     IHTPIITTSDAEGAGAMFQVTSLDIEELSKSGTLIDYDKDFFGRKAFLTVSGQLNVET
                     YCQALSRVYTFGPTFRAENSNTTRHLAEFWMIEPEMAFAHLDDNIALATEFIQYLVAT
                     AIRDCAEDVAFFDQRIQPGLRESLERIVQSSFARMSYTEAIRELEASNANFEFKPYWG
                     CDLQSEHEKFLTEKVAGGPLVVTDYPKEIKAFYMKQNEDGKTVAAMDVLVPRFGEIIG
                     GSEREWRLDLLEKRMEELGLDKEPYQWYLDLRRYGSTPHSGFGLGFERLLLYITGMTN
                     IRDVIPFPRAPRQADF"
     gene            6410..7702
                     /locus_tag="PLQ30_05840"
     CDS             6410..7702
                     /locus_tag="PLQ30_05840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HPW02003.1"
                     /translation="MKLLTAIKRHQTCINKASLLIIAIISCFFIFDFPAAANSIESFS
                     RLGVEIPNPPSLNARSAILIDAATGTVLFEKEADIRLPPASLTKLVTLHIVMEEIKSG
                     RLSPDEIVEIDPQDCSPSIPYGSSLMYLQPGMKVSVKDLMLGAAVVSGNDAAYSLARR
                     IAGSNEEFAEMMNKEVKAMGFQSLCFVEPSGLSEFNLVTARDFAFFCKKYIELHPKAL
                     KELHSVHSIQFPRPEHATSQYRPNGIVLQYNRNPLIFSYEGADGLKTGYIIEVGYNMA
                     ATAMRNGNRFIAVLLGGSSTPYADGAALRTKDTKALFDWAFANFATARPGYIMPKPLR
                     VWFGKSIWIDPEPEGEAAATVPVVYKDRVTARIETVKGIKAPVYKGQKLGEIVYEIDG
                     TKINSVNLVSGQDIPRGNLFRTLLDSIQYFFAYIFGKL"
     gene            complement(7775..8809)
                     /locus_tag="PLQ30_05845"
     CDS             complement(7775..8809)
                     /locus_tag="PLQ30_05845"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPW02004.1"
                     /translation="MKNVVAALFIITILSFPFSAFGEITLKLAHLNPQAPFEVVSAAM
                     SAVFKSEVESKSDGQIKVDIYPDGVTGKEIETLIQVKSGQVQSSISTSGAIAQFYPLI
                     DVTNIPFAFSSYNVAYHVYDGQFGKELAADIEEKAGFHVLGFAESGGFFAITNSKRPI
                     RSPGDLKGLKVRTMAIPLHQEIIRALGATPVTIAWSELYASLLAGVVDGQMNPVSIIV
                     MDRLYEVQRYATLCNLIYSPYVWVINMEFYNSLSKELRIVVDDAARTAIVAGRGLSRI
                     IDSTERGLPMLSGKMQVYVPSGAVMKQFKDIVVPAANKFLLTQYKEEGSIWMNKFVAA
                     IDKAEKELGY"
     gene            complement(8820..9230)
                     /locus_tag="PLQ30_05850"
     CDS             complement(8820..9230)
                     /locus_tag="PLQ30_05850"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPW02005.1"
                     /translation="MFQVPTKTQYAIRALVHLVHVKSASVASIAEAECIPSKYLEAIF
                     GKLKNAGLLIAERGRNGGYRLAKDPALVRMSEVVRATEGEIRIVECVDNAAICLLGQG
                     CLPRHFWLGLKDAVDLYLESVTLAEIANPDMSSA"
     gene            complement(9290..>10338)
                     /locus_tag="PLQ30_05855"
     CDS             complement(9290..>10338)
                     /locus_tag="PLQ30_05855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPW02006.1"
                     /translation="NRAAIGGIRATHSDPAKIRICLESIRLFSSMQEKYGLDVEWRQG
                     GYLYLAFDEATEQQFCALLEKQKAAGLDIDWVGSETIYRLAPGLRTDDLRGGTYSPQD
                     GYASPLMAASAFHYLALAAGVSFFFNTKVKAIHREGERISSLEAGSESFSADLFVNAA
                     GAKAADIAKIAGFDLPVFPDCHEAGVTEPVERFMEPMIVDVRSDAESGNYYFYQAATG
                     QIVFCITPRPQIWGRDKDSTSTFLPLVVRRMLELYPRLRNLRVRRTWRGMYPMTPDGL
                     PIIGYPDLAVNFLQLVGMCGQGFMMGPGLGSIIARSIAANGCHERPEGFTDYGFLFEE
                     LSINRSFDHAELLS"
BASE COUNT         2929 a         2384 c         2209 g         2816 t
ORIGIN      
        1 cgattgaaat cagcacgcct ttggtgggct cgatctatgc tggaaatatg gccgtttctg
       61 ccctcgccct ttctcagcta gacatcgagc tgaatacgga acacatgctt cagggctttt
      121 cttcatcatc tatgcccgcc cgatttgaga tagtctcaca ttctccattc gttgtgctgg
      181 atggagctca tactccagaa tcgattcgaa ccaatctttc cacattccta gagctttcga
      241 aggctccacg cattttattg ttcggatgcg ctcatgataa aaagcatgat gaaatcgcga
      301 aaataatctc accatatttc gacgaaataa tcataacaag gccaggcaca tttaaacaaa
      361 gtgaaccagc tattgtattc gatagttttc aaaaacagat gtcgaatagc attatatcag
      421 atcgctatct aatcgaagat acaaatagcg caatagccaa agctcttagc gatgcaaggg
      481 agaaaaatgc tagccttctt gtaacaggct cattctatct gtgcgcagag tttaaaaagt
      541 cgagattatg ctctgaatga agcctatcat tcctcccaaa atacctccaa gccatgtaat
      601 ccaagccaat tcttttttta ctacttgaag aacaatgcgt tcgacctctt tcatgtccag
      661 gctatcgatc cgttctgaga ccatcgaagt tatatcgaat gcgtctatta aagtcgatat
      721 tctggattga atgagttcga ttaaaccatc ggtaagtgat accgaaattc tctcgatttc
      781 ggaatcttga attttgagaa tatccgcgaa ggtcttgcct tttgaaacat cctttaagac
      841 ctcaataaaa atcgatccca ttgtattgga agcgctgcta tcggagacat gcatggttgg
      901 aacgatgatt gaaaagacaa atccagatat ggcttcctca tttgacctta ttcctagaat
      961 ttccccaaac tccagatccg caagagagtg atatatggct tcagctcgca gtcgcaagtc
     1021 ttcggaagaa tcctttagag acagaacaat ctgttcagat acaactctgc tcttagagcc
     1081 gatggagtca tcagcgcgct gatatatctc tggaccggtc tttgtggttt cagcttcgtc
     1141 cttcgaacgc caattaataa ttgccctgga aatagttttg gaaatacttt gaggagtgga
     1201 gggatctttc aagatgcgtt ctatggtctg tattagatcc tcgatgttct cgggcatggc
     1261 ttgagcaatt ttggcatcgt atcccgcaat agagataaaa agcctttgga tgggccctag
     1321 tctatccaat gctcttttta caaatgcttg agcttcggtt tctagccttg ccctaaatcc
     1381 ttcactatga aaaaaactat cgatatagtc cagaaaccag gtatatactt gcggaacaag
     1441 agcatctgta aaagccataa tcgttacatc cggaggaagg tctataacgg ctttcaaaag
     1501 cggattaaaa cgtgatgatt tagatttcga ggattgaagg ctctgctcat ggttagattc
     1561 ggaaagcact cttgtttgca aagtagaaca agctttgagt acagatgcta taaattgttc
     1621 tttagaaact aattctttaa aggagagctt gccaattcga ttcaggaatt cggcgaagat
     1681 tccctctatg gagctttgaa cgctcttgcg ctccactaga tgttgtaaag cctcaattat
     1741 attgcgttga agcgcagaga cttctgaatc tattgtagtt ccattaccag actggacttc
     1801 tttcgttatt ttctggccat ttgattccag cagaagaagt gagggctcat catctttttt
     1861 ttcctccagg cctggctcac caatttggga aaaaatccgt cctgcatcct gattgaaaaa
     1921 actagctaaa gccgatttga tagccttcaa gatagtctgc tttatatcag cagactgaat
     1981 tctttgagtg atcacctctg cagtcaggag ctccttagag actgtctctc ctaaacttac
     2041 agcaagcttg tccttctcgc gcggcagcaa gcctggagta aagggcaagg caatgcccgc
     2101 aaatttgatt gtcttgtatg gtctgaaaag catctttata gcgagccagt tagtaaaata
     2161 cccgataatt gcaccgacaa gcggtggaag gatccatgct ttgaaaaatt ccatgcttat
     2221 tccatcctga gagctgcatt tcttgcttgc ttttcgctgg agtaggtcat tacctccagg
     2281 gctggaaccc atccggtttg cccagcttct tgtattctga accagagtgt ccctcgatcg
     2341 ctttccgatg tactatactt gctctctatt atccgcaaaa cggtcccttt tcggagaatg
     2401 gagaggtttt gtgaactatt ggtaggatct tttttcattt gacaatactg ggaagatata
     2461 acaagccatt gcgcatttcc gcccagacta ttatcctccg gtagccgcaa gcgaggtcta
     2521 catcctgaca tggcgcacaa aacaaatgaa gaaattagaa ttaatgatga ataaacaaat
     2581 atcttcttca tactgacata ccgcttgatg acgctgcgaa ggaaaccatg tagtattaaa
     2641 caataactat gaatatgaca atcggcaagg caaaagcaaa gccgtttttg ttcttggtgt
     2701 ttatggtttg caaacttggc gctacgcagg caatcggggc tcttccggct ctggaattcg
     2761 gcatgggatt tgcttatgct aggacgaaag tcgaaagcgc cgcaacagga tatcaccatg
     2821 gatggaatgt ctcagccgat gcgaattggt tattccgtat atctctcgaa gatgagaatc
     2881 ttataaaagc aaaagcagaa aaagagactt catcgcgcgt caatatgata ttttcaccgg
     2941 gactgcatgc tcaatttata accatgtcag cttctaactg gctcccggat ggttcacgct
     3001 atagagcatg ggattctatt gggcttgggc cagaactgaa tcttggctta gaattcacta
     3061 attccacaac gatgcataaa aatggcttct tgttttctat ggcatatctt gcaagctttt
     3121 ctaattatac acaaacaacg ctttattcgg catataattc gtggtttttg aaagtatctt
     3181 ggaatgccag aattgacaat ggtgcagctt ttttcgcagc cctgcccttg gaatatgcat
     3241 tcagagcgga tggcaacagc ctattatctg ggcttgtaat tgggatgaga tatgaattct
     3301 aaaactctac atacatccag caaatataca tatatgttta tgtgcataaa gatgaaagat
     3361 ttcgcaaaag agactagcaa atatggcggg ttaatgaagg gagcattttt tctgctcgtt
     3421 ctatggccac tattagcaag ctgtgagatt ttagctccaa ctaatgccac attcgaaggg
     3481 gaaaaatatg cggttgtggt ggggataaat gattacatcg acaatacaaa caatctgatt
     3541 agggatttag atttctgtgc cgccgatgcc gaggccatga aagacatgct gacaggcgca
     3601 ggctggcagg tggaacttat cacggccgaa gcgaacgaat caataaagaa aaatgcaacc
     3661 aaagaaaaaa tcaagacggc atttcaaaag gacaaagatg gcttgacagc gtttctcttc
     3721 tatttttccg gccacggtga tgtacatgaa gatcaggctt acattgtgcc aagcgatgcc
     3781 tatcgatctg gcaattccgt agagaaatca agcatgatct cagctactga attgtctatg
     3841 tggctaaagg atttgaatgc caacaaaatc atgatcttcg actcttgcta ctcgggcgga
     3901 tttgtggatc cgggagattg tatagatgct gctccgggaa acattgaatc tgatcagatt
     3961 tccacaaata tcgaaatgtt tttccgcttt ggcgagatac tcgctcagaa ctctgcttcc
     4021 gcagccagaa gcccatctac ggctcctctt gtgatatccg cttcaggctc gaatgaagcc
     4081 tccatggaag acgccaatga aagaccaact catggaagtg gccatggatt ctttacttat
     4141 tattttcttc aatctgccga agcatcaaaa gatggctcaa tgaaaggaga tcttgatggc
     4201 gatggaatac tctcctgtct agaagcatat aattatgcaa aaaatgcgct cgaaaaagac
     4261 tggatagctt cccagctacc gcatattaca ggcggtctta gggatttcgc tctgatcgat
     4321 ttgcgctagg acctataaca gataaggcat gataataatt cgaatagcct tcaatcgata
     4381 aaatccgtat cttcctttga gaattcgggg aaaaaataga caagtacacc ggaactccgc
     4441 acgcgcacct gtgcaatgct cttctttgca agattatcga gttggcgctg tgcctcttct
     4501 atagggatgt tggcctcaat cgccacttcg cctgccgtga ccatgccatt gttagcctta
     4561 gcgaggcgaa gaataacccg ctctggagtt tcggactgtc gaatatgtgg aatgaactca
     4621 ccccattggc catagttttc atgtccaata gcctctctcg cagaataggc aagattcgct
     4681 tctcgaactt gtcgaggcag agtaataaga tcatataggc ctcctaatcc tcctagacca
     4741 cctgtaaaca tccacaaaag cccagttccg gtcttcccta aatagaaacg atggaagccc
     4801 agggctccaa agccagaaat aagccagagg aaataggctg ttgtaagatt gtatctcacg
     4861 ctccgctccc aaaaagatct taattaaact ctatggtatt tgaccattat agctatgtcg
     4921 tgctaaaaag gagtatatga attttatcgc atgacgaaaa gccatgcacc actctttcta
     4981 tggagctttt catgactctt cttccaacaa taaagcaact tctttccatg gaagctgtag
     5041 gtcagcctgt gatcatcagg ggatgggtcc gcactaaacg cgagacaaaa caagctattt
     5101 ttatcgaagt caacgatggt tcttgcctgt ctaatatcca atgcgtcctg gattcctctc
     5161 tcgaaaaaaa ccaatcgata aatgagacta tcttgagatt atctacggga gcaagcgtgg
     5221 agatacatgg agagctcgtt ccctctcctg ctaagggtca aaaggtagaa gtcaaatgcc
     5281 aacagatcaa gctaattggt gaagcaccat cggagcgcta tccgcttcag aaaaaagcac
     5341 atagcctcga atttctgcgt gaaatcgcgc acctcagggt gcggacaaat acttttgctg
     5401 cagttgcccg cgttcgaaat cggatggcat ttgccataca ccagtttttt caagagcgtg
     5461 gcttctatta catccacaca ccaatcatta ccacaagtga tgccgaggga gctggcgcaa
     5521 tgtttcaagt tactagcctc gatatcgaag aactctcgaa atcaggtact ctcatagact
     5581 acgataagga tttttttggt agaaaggctt ttctgactgt atcagggcaa ctaaacgtcg
     5641 aaacctattg ccaagctctt tcgcgcgttt acactttcgg ccctaccttt cgcgctgaaa
     5701 actctaatac cacgcgccat cttgccgaat tctggatgat cgaacccgag atggcatttg
     5761 ctcatctaga tgacaacatc gctctggcaa ccgagttcat tcaatatctt gttgccacag
     5821 ctatccgaga ttgtgccgaa gatgtagcct tctttgacca gcgcatacaa ccaggcttaa
     5881 gagagtcctt agagagaatt gtacagtcaa gctttgctcg aatgagttat accgaggcaa
     5941 ttcgagaact cgaagcctcg aatgcaaatt tcgaatttaa gccctattgg ggctgtgatc
     6001 ttcagagtga gcatgaaaaa ttcttaactg agaaggttgc tggcggaccg ctggttgtaa
     6061 cagactaccc aaaagaaatc aaagcctttt atatgaagca gaatgaggat ggcaagactg
     6121 tggcagcaat ggatgtgctt gtacctcgtt ttggcgagat catcgggggt tccgagcgcg
     6181 aatggagact cgatcttctg gaaaagcgaa tggaagagtt aggccttgat aaggaaccct
     6241 accaatggta tttagatctg cgacgctatg gctctacccc tcattctggc tttggtcttg
     6301 gattcgagcg gctactcctc tatataacag gaatgacaaa cattcgagat gtaatacctt
     6361 tcccaagagc accgcgccaa gctgatttct gatcaacagg aaaacatcga tgaagcttct
     6421 tacagcaatc aaaagacacc aaacatgcat aaataaggcc agccttttaa ttatagctat
     6481 tatctcatgc tttttcattt tcgattttcc ggctgcagcc aattcaatcg agtcgttcag
     6541 tagattgggt gtcgagattc ccaacccgcc ttcgctcaat gcgagatcag cgatacttat
     6601 agatgccgca actgggactg tgctattcga aaaagaggca gatataagac ttccgcccgc
     6661 aagcttaacc aagcttgtca ctcttcatat cgttatggaa gagataaaat caggaagact
     6721 ttcaccggac gaaattgttg agatcgatcc gcaagattgt tcaccttcca taccttatgg
     6781 ttcttcgctc atgtatctgc agccaggaat gaaggtgagt gtgaaagatc tcatgctggg
     6841 ggccgctgta gtatcaggaa atgacgcggc ctattcactg gctcgccgta ttgcaggatc
     6901 gaacgaagaa tttgccgaga tgatgaacaa agaggtcaag gcaatgggct tccagagcct
     6961 ttgctttgtg gagcccagcg gactttcgga gttcaatctt gtaaccgcga gagactttgc
     7021 ttttttctgc aaaaaataca ttgaactaca tcctaaggca ctaaaagaac ttcattcggt
     7081 acattcaatt caattcccta gacctgaaca cgcaacatca caatatcgtc ccaatggtat
     7141 tgtccttcaa tataaccgca atcctcttat tttcagctac gaaggagccg atggcctcaa
     7201 aacaggctat ataatcgaag taggatataa tatggctgct acggcgatga gaaatggtaa
     7261 tcgctttatt gccgtgctct tgggaggatc ttctacccct tacgccgatg gcgctgcctt
     7321 gcgcacaaag gacacaaagg ctctcttcga ttgggcattt gcaaatttcg ctactgcaag
     7381 acccggctac ataatgccaa agccacttcg tgtctggttt ggcaaatcaa tttggataga
     7441 cccagagcct gaaggtgaag cagcggctac ggtccccgtt gtttataaag accgagtaac
     7501 cgctcgaatc gagactgtta aaggcatcaa agcccctgta tataaaggac aaaagttggg
     7561 tgagatcgtc tatgaaatag atggtacgaa gataaattcg gtcaatctgg tttcaggtca
     7621 agatattccg cgtggtaatt tattcaggac tctcttggat agtatacagt atttctttgc
     7681 ttacatattc ggaaagctct gaactcatcg aaaaaatgac ctgttctaag aacaggtcgg
     7741 ctctaaaccg gacgccagat aaagaaataa gatttcaata cccgagttct ttctctgctt
     7801 tatcgattgc ggcaacaaac ttgttcatcc agatactgcc ttcctccttg tactgcgtaa
     7861 gtaagaattt attcgccgca ggaactacta tatctttgaa ttgtttcatt actgctccgg
     7921 atgggacata gacctgcatt ttcccagaaa gcatgggaag acctctttct gttgagtcta
     7981 ttattcttga gagtcccctt cctgccacaa ttgctgttct tgcggcatca tccacgacta
     8041 tcctgagctc tttcgatagg ctgttgtaga actccatatt gataacccat acatagggag
     8101 aatatatcag attgcatagt gtcgcatatc tctgtacttc gtatagtcta tccatgacta
     8161 ttatagatac cggattcatc tgaccgtcca cgacccctgc tagaagactt gcatatagtt
     8221 ccgaccaggc aatggtaacc ggagttgcgc ctagcgctct gataatctcc tgatgaagtg
     8281 gaatagccat agtcctcact ttgaggcctt ttaagtctcc aggtgatcta attggcctct
     8341 ttgaattcgt gatagcgaaa aatcctcccg attccgcaaa accaaggaca tgaaaaccag
     8401 ctttctcttc tatgtcagca gcaagctctt tcccaaactg tccatcatat acatgataag
     8461 ccacattgta actcgaaaat gcaaaaggga tgttagtgac atcgataaga ggatagaact
     8521 gagcaatggc gcctgaggta gagatagagg attgtacctg cccggatttg acctgaataa
     8581 gtgtttcaat ttctttccct gtcacgccat cagggtaaat gtccactttg atctgaccat
     8641 cggacttgct ctccacttcg cttttgaata ccgctgacat agcagcggat acaacctcaa
     8701 aaggtgcctg aggattcagg tgtgccagtt ttagagtgat ttctccaaac gcggaaaatg
     8761 gaaatgatag aattgttatg ataaacaagg cagctaccac atttttcatg gatgcctcct
     8821 taagccgatg acatatcagg attcgcaatt tctgctagag taacggattc gagatataga
     8881 tcaactgcat ctttgagtcc tagccaaaaa tgccgaggca gacagccttg acctaataag
     8941 cagatcgccg cattgtcgac acactcgaca atccttatct cgccttccgt tgcccgtacc
     9001 acttcgctca tccgaacaag cgccggatct tttgcaagcc tgtatcctcc atttctaccc
     9061 cgttcagcaa tcaagagccc tgcattcttc agttttccaa aaattgcttc taggtattta
     9121 gacggaatac actctgcttc cgcgatactc gctacacttg cggattttac atgcaccaga
     9181 tgtacaagcg cgcgaatagc atattgtgtc tttgttggaa cctggaacat taaaattcct
     9241 actaaataag tctgaatatt taatatcgca ttaatggaga ctttgtcaat cagctcagaa
     9301 gctcagcgtg atcgaagctg cggttgatcg aaagctcttc aaacaaaaag ccataatcag
     9361 tgaaaccctc aggcctttca tggcaaccat ttgcggcaat agatcgtgca attatactgc
     9421 ccagaccagg ccccatcata aagccttgtc cacacatgcc gacaagctga aggaaattca
     9481 cggctaaatc ggggtaacct ataatcggaa gaccatcagg agtcatggga tacatgccgc
     9541 gccatgttct tcgtactcgt agattgcgaa gcctaggata cagctcaagc attcggcgaa
     9601 caacaagtgg aagaaaagta gaagtagaat ccttgtctcg accccatatc tgtggacgag
     9661 gagtaataca aaacactatc tgcccagtag cagcctgata gaaataataa ttgccggact
     9721 cggcgtcgga gcggacatcg acaatcattg gttccataaa acgctcgacc ggttctgtca
     9781 cacctgcctc gtggcaatcc gggaaaacag gcagatcgaa acctgcgatt tttgcaatat
     9841 cagcagcctt tgcgcctgcc gcattgacaa acaggtctgc cgaaaaactc tcgctgccag
     9901 cttcgagcga agagattctc tcgccttctc tatgaattgc ttttaccttt gtattgaaga
     9961 aaaaagatac accagccgcc agcgcaaggt aatgaaaagc acttgcagcc ataagaggtg
    10021 aggcataacc gtcttgaggg ctataggttc ctcctctcag atcatcggtc ctaagcccgg
    10081 gcgcaagtcg atatattgtc tctgagccca cccagtcaat atccaaacca gcggccttct
    10141 gcttttccag aagcgcgcag aactgctgct ccgtagcctc atcaaaagct agataaagat
    10201 aacctccctg cctccattcc acatccaatc catacttctc ttgcattgaa gagaagagcc
    10261 ttatcgactc aagacaaata cgaatctttg caggatccga gtgagtagct cttattccgc
    10321 ctatagcagc ccgattct
//
LOCUS       DAOMZL010000060        10211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_6193_length_10211_cov_11.779047, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000060 DAOMZL010000000
VERSION     DAOMZL010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 10211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10211
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6193_length_10211_cov_11.779047"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PLQ30_05860"
     CDS             <1..439
                     /locus_tag="PLQ30_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02007.1"
                     /translation="AIAGHGRPYNCHKFYHRCSRNKGTCLKDDFTGDLAHGPYEQGWI
                     FFLDPDAHVRISNSELRKVFIDIVNDNAKFHDLKVGIPSSLTYRDIELSNVTVMGQWP
                     FTIKGSHITVNNSDYLFLQPIGNSTVTLNNSHMCEFIPRDLIL"
     gene            909..2579
                     /locus_tag="PLQ30_05865"
     CDS             909..2579
                     /locus_tag="PLQ30_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW02008.1"
                     /translation="MLDESEAILFGPGPEAYHDQLKSNILKCAEQEILRTIVIHGEEP
                     GIASSLSHFEKDGSVATIVTDLQTWNQLCFYGLTMQPYILDEHGWLFKLASGRVLQFL
                     PTPYLPQPSAFVTYDEKTKTLLTGGLCSSYSTNLSLYEYDGFLDQLTRFHRVNMPSRE
                     LLAPVLEALSLRDIERILPSYGAIIHSDIKSIFNHLSSIECGELFQIGLADELYARAV
                     KEGASAEELALLQQELHKLRKLNEELNNSIEVSRDRAIRDEVTGLYSEAFYNSFIEEE
                     AAIRISETGPEDHVLAIISLDENIAQIEYKYGSKEVEALLRGVARILLQNLPKTAMAF
                     RLHGATMAIWIPAISFNEAVNLFDRMRYEIETSKVFIEPITVSIGLSTLAEASESQPE
                     LEKIALAMTDIGIRRLRLARKKGGNTVYFGTTEEEEAQSLANIMIVDDDEANVEVLKT
                     YFVNQGYAILTASDGQEALSLLGKEAVEIVISELMVPKIDAYRLKESMLTKTSTKDIP
                     VIVMSHLKTELSVRRAYDLGILYYLQKPIMLEELLGIVKVLSQTGKDE"
     gene            2576..5629
                     /locus_tag="PLQ30_05870"
     CDS             2576..5629
                     /locus_tag="PLQ30_05870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW02009.1"
                     /translation="MTIASDYLLIAVIFFLWASLYAMITTLLNKSHIMIKAEERRNTL
                     KQISQVITPSFKLSKRKIRSLLENILLVSQSVVISPIQKQRVFTTAPIQRFEHTLLRH
                     LFSHNRFIRMNAAKGLSIIGSESARKAIERALLQEKKFAMKLYLANALSDIGDSRSIQ
                     SLIKSLYGAHYWYRSKVNMLIAGFPNAMKEIGPEYYSSSDIEIRELLVDLAAIYPSFK
                     LKQYIIEIVQKFPLEIEKQIKTAELLPQKCCYYCVYGQKEADDLHRQCPYRSKVANDF
                     HCRRFRNLMMRHFSASNYHRLMVRATQALEKLYPEELSATLYLEHGDKEIQSIAVRSL
                     GHSPSLDHFSKLLFYLECDSTAKAAHEGLSELLSTHSNFIPKTIDAFVHSTGVLHQRL
                     ADVLSYRIEYIIAKLVGYDHDVAKGIIDELIRMGHVTEIIEFLKSNKNIELEAELIAI
                     IKPQLQDNALLARECGLFLPDALLEKLGLERLSRSEPDRQEKLNKKMIHWMYGIIAVA
                     VCFFPIIYVVRFYPRIATLSFIENMKYFVLNFNVTFAYYAIAVNLIYLVLLFFSRLNI
                     RREAKLWELKKATMFFKPRMLPSVSIIAPAYNEERTIIQSANSLLNLRYPDHELVIVN
                     DGSKDATLKTLIDYFDLKRTDFPYEQRLPHYPIRGIYTNPSIPNLVVVDKENGGKADT
                     LNAGINVSSKEYFCGIDADSLLESDALLKVAALTLDYGVETPALGGNVFPINGCKVDK
                     GKIERIGVPNQWLARLQSVEYMRAFMCGRLGWDYLNSLLIISGAFGLFRKERIVEVGG
                     YLTSKSQYQRDTVGEDMELVVRIARSMHEAKRPYRISYAFNANCWTEVPEKLSSLKKQ
                     RYRWQRGLVDIMHFHKKLIFNPKYGLMGILAMPYFLIFEMIGPLFEIQGYVMVFLAAI
                     FGILSARIALLLFFGSVLMGIFISIMSLKTAERHDIYYDYRDTFRLIGTAIVENFGPR
                     QLFSTWRASGFVKAMQKPQGWQKFERKGFVGKEPSAASTEGIVHL"
     gene            5626..8871
                     /locus_tag="PLQ30_05875"
     CDS             5626..8871
                     /locus_tag="PLQ30_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02010.1"
                     /translation="MITRITERWRTRLLWIIGIALLALSTLLIALPFVLWRCEPYHPL
                     EIWVVDKTVPYTDFREHAGLFWLLDNNKIAKPDTKKLYNVRTDYYGFYPVSNSPANYE
                     WRSNSLPETSKKPDLIYIADTYGVYRDDYMQKKSPEDNSSLLYGGLDGNDCKIIQSAL
                     GQGNTFIAEFNTAASPTNAKNRDLLGRLIGLSWRGWIGNYYDDLAPHGSVPAWIVQNW
                     ESALNQKWTFSGRGIVLLNERGDIEVLTEKEDISPKGMKMVFNAPWDSEIGVKKPVSY
                     RYWFEWITADPLLEEVAHYELNLKPEGKKKLDRLNLPTTFPAIVRYKNEQYTSWYFAG
                     DFADLQFSGTPYRVKGIQAIKRILTDETVEDNSCFFWKAYAPIMNYILEENRKQRAEF
                     NAIQKPIEVRVPVRAIREGFQLTSPSGDWNTIFVRSVNLGTAEPGKFFTEFPNEIDTY
                     LRWLAMIADMGANTVRIYTLLPPEFYRALAIYNMTHSEATLLLLQEIWPEEHPPGNDF
                     LQPLYQEAFKKEIGYVIDAVYGRANIPERKGRAWGIYSTDVSKWLLGWLVGRELESVE
                     VLQTDANHQGIAYAGKFISAGKAASATEVWLAESLDAVSIIEYESYQTLHPVGIVSWP
                     TLDPIQHDTEWDPITGKKNRWNDCSSIQIEHLETSPLMTAGLFGAYHIYPNYPDFIVN
                     EPSYNLYKDSVGILRYGRYLEEFIKTHVRYPAIVAEFGIANGAGVAHLSPDGLHHGGS
                     DEATAGRDILRMYDAIQKTGYTGASIFEFMDEWTKKTWIQEPFMIPFDRRVLWHNAVD
                     PEQNYGLIANESIAPTAPEKTIQGSGALMQLDIAHDATYLYLTATLSADNLINGNEIL
                     IGIDTYKRAVGQMAWPRGLGTTASGMEFLLEIKPQEMRLQVIPSYNIARARYASTLMH
                     SGAFSDIDMLVNGKVITKDGTMIPEKRFNASILRQGEFDESGNLWNVQGNILRVRIPW
                     NRLNVTDPSSLKVLDDSRSIDNPVTDQLKTIVTDGFVFDALVLDSTSGKVIGKLEANI
                     DAPYIWSGWEDTPPYRERLKKSYFIVRDLWGAQAEEEMTIRKEFIEAQSGR"
     gene            8962..>10211
                     /locus_tag="PLQ30_05880"
     CDS             8962..>10211
                     /locus_tag="PLQ30_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HPW02011.1"
                     /translation="MMKRIFHPSILSVAMIAIAVDLAFSSALTKPIAKKEYRNVGYVF
                     HKNCDINTLDVTKITHLNYSFGLIYNREYEELGNETNPDVIVPHDFDDSLLHTIYLSD
                     KVESDLSRIYILKEKNPVLKILLSVGGWGCRGFSDAAASNDARKAFAQSCRKIIDRFG
                     LDGIDLDWEYPVIGGWGSIKSRPEDKQNLTLLLKEVRKTIGNDKLLTIAGSPSVSFSN
                     GEWIEFQNVIDLLDYINIMTYDFGNGTCYFDSALYPSKTWKTKSDHDDYNIDMAVKLY
                     LKNGCPAEKINVGISFGASIPKVVVSNNKLWKAISKKLNTVGFFTSKDPLIKKVKDLL
                     EDKNGFHKEWDEQAKTMYISTDLLNVRQFVMSYIEPQGLSERTKYIKDNNLGGIMFWE
                     FGADYDNSLVSQIARELDINRLGE"
BASE COUNT         3030 a         2157 c         2235 g         2789 t
ORIGIN      
        1 ggctattgca ggtcatggga ggccatacaa ctgtcataaa ttctaccatc ggtgctctcg
       61 gaataaaggt acctgcttga aggacgattt tacgggtgat cttgcgcatg gcccatatga
      121 acaaggctgg atattcttcc tcgatccaga tgcccatgtc agaatatcca attctgaatt
      181 gcgcaaagta tttatcgata tcgtcaacga taatgcaaaa tttcatgatc taaaagtggg
      241 aattccatca agccttacgt atagggatat agaattgtca aatgtgacgg taatgggaca
      301 atggcccttc actattaaag gttcacatat tacagtcaat aattccgatt atctcttttt
      361 gcaaccgata ggaaattcga ccgtgactct caataattct cacatgtgtg aatttatccc
      421 aagggatttg attttatgag gacaatggaa tttaataatg ccaagtggac tattgcaggg
      481 gaaatcatag gagatgttgc ataccattga acgtttagca ttatctcgcg acataccttt
      541 gctgtaaaga acaaagtggc ataagcagat atggcagatg ctgattttca tttttcgaca
      601 cgattttcaa gattagcttt gataagaagg atatttttgg acacaatcta tcataaggct
      661 actctctttt cctctattaa aattatgttc atcaactctt atcctgccca tcctgtcgat
      721 atattaacct atcccatcta aacattctgt ctattacttc cgaccttgac agctctactc
      781 tctttatctg aaaattaata tgaaggtagg ttattaaaat tatcgatact attgtagacc
      841 ttggtggagg tttttttcga ataggagctc caagtctcgt aggagcgctt cagtccaata
      901 gctatctttt gctcgatgaa tcggaggcaa ttctatttgg accagggcca gaagcttatc
      961 atgatcaatt gaagtccaat atcctaaaat gtgccgaaca ggaaatactt cgcacaattg
     1021 taatccacgg cgaagaacca ggaatagcat cgtcgctatc tcatttcgag aaggatggct
     1081 cggtcgcaac tattgttacg gatttacaga catggaacca gctctgcttt tatgggctta
     1141 ctatgcagcc ttatatcctt gatgagcatg gttggctttt taagctcgca tctggtcgcg
     1201 ttctgcaatt cttgccaact ccttatctcc ctcagccaag cgcctttgtc acttacgatg
     1261 aaaaaacaaa gacattgcta acaggagggc tctgctcttc gtattctaca aacctttctt
     1321 tgtacgaata cgatggattc ctcgatcagc tcacacgttt ccaccgagtg aatatgccct
     1381 cgcgtgagct acttgctcct gtactagaag cgctcagtct acgcgatata gaacgcatct
     1441 tgccatctta cggtgctatc atccatagcg atataaagag tattttcaat catctctctt
     1501 ctatagaatg tggagaactt ttccagatcg gcttagccga tgagctttat gcccgagccg
     1561 ttaaagaggg cgcaagcgct gaggagcttg ctctgcttca gcaagaactc cataaactgc
     1621 gcaagctcaa tgaagagctc aataatagca tagaggtttc aagagaccgt gctatccgag
     1681 atgaagtcac gggtttgtac tcggaagcat tctacaactc attcatcgaa gaagaagccg
     1741 caatacgcat ttcggagaca gggcctgagg atcatgtgct ggcgataatt agcttagatg
     1801 aaaatatcgc tcagattgag tataaatatg ggtctaagga agtcgaagcc ttgctgcggg
     1861 gtgttgcgcg catccttctc cagaaccttc ccaagactgc tatggccttc aggctgcatg
     1921 gtgcaacaat ggcgatttgg attccagcaa tttcctttaa tgaagcagta aatctcttcg
     1981 acagaatgcg ctatgaaata gaaacatcga aagtatttat tgagcctatc acggtatcga
     2041 ttggtttatc gactctcgct gaggctagcg agagtcagcc agaattagag aaaatagcat
     2101 tggcaatgac ggatatcggc attcgtcgtc ttcgccttgc acgcaaaaaa ggtggtaata
     2161 ctgtttattt tggcactacc gaggaagaag aggcacaatc cttagcaaac attatgatcg
     2221 tagacgacga tgaagcaaat gtcgaggttc ttaagaccta ctttgtcaat caaggatatg
     2281 ccatattgac cgcctctgat ggccaagagg cgctatctct gctcggaaaa gaagcagtag
     2341 aaatagtcat ttccgagctt atggtgccaa aaatcgatgc ctatcgactt aaagagtcga
     2401 tgctcacaaa gacttctacg aaagatatac cagtcatagt tatgtctcat ttgaagacgg
     2461 agctatcggt gcgcagagcg tatgatcttg gcattctgta ctatcttcag aaaccgatca
     2521 tgttggagga attgcttgga attgttaaag ttctaagtca gacaggaaaa gatgaatgac
     2581 tatagcttca gattacttat tgattgcggt catttttttt ctttgggctt ctctctatgc
     2641 aatgataacc actctgctca ataaatctca catcatgatc aaggccgagg agcgtaggaa
     2701 cacgcttaag cagatttctc aagttataac tccctccttc aaattaagta aaagaaaaat
     2761 ccgttctttg ctcgaaaaca tcctactcgt gagtcaaagt gttgtcattt cgcctatcca
     2821 aaaacaacgt gtgtttacaa cagctcctat ccaacggttt gaacacacgc tgctgcgtca
     2881 cctttttagc cataaccgct ttattcggat gaacgccgct aaagggctct ctataatcgg
     2941 ctccgaaagc gcacgaaagg caatagaaag agcacttctc caagaaaaaa aattcgctat
     3001 gaagctctac cttgctaatg cactttcaga cattggcgat tcaagatcca tccaatctct
     3061 tattaaaagc ctttatggag cacattactg gtatagaagc aaggtcaaca tgctgattgc
     3121 aggtttccct aatgcaatga aggagattgg accagaatat tattcaagct cagacatcga
     3181 gattcgcgag ctcctggttg accttgcggc tatttatcct tcttttaagc tcaagcaata
     3241 cattatagag atagtacaaa agttccctct cgaaatagaa aagcagataa aaaccgctga
     3301 attattgccc caaaaatgct gctactactg cgtgtatggt caaaaagaag cagatgactt
     3361 gcatagacag tgcccctatc gaagcaaggt tgcaaacgat tttcattgtc gaagatttag
     3421 aaacttgatg atgcgccact tttcagcctc aaattatcat cgcctcatgg tgagggccac
     3481 gcaggcatta gaaaagctct acccagagga gttgagcgct actctatatc tcgaacatgg
     3541 agataaggag attcagtcaa ttgcagtgcg ttccttgggg catagtccaa gtttggatca
     3601 tttcagtaaa cttctatttt acctagagtg cgatagtacg gcaaaagccg cccacgaagg
     3661 tctttccgag ctccttagta ctcactcgaa tttcatacca aagacaatcg atgcttttgt
     3721 acactctact ggtgtgctcc atcaaaggct ggcagatgtg ctctcctata gaatcgagta
     3781 tataatcgca aaattggtag gctatgatca cgatgtagcc aaaggcataa tcgatgagct
     3841 tatcagaatg ggccatgtca cggagatcat cgaattctta aaaagcaata agaatatcga
     3901 gctcgaggca gagcttatcg ccatcattaa accgcaattg caggataatg cgttactcgc
     3961 aagagaatgt gggctttttt tgcccgacgc tctcctagaa aagctaggcc ttgagcgact
     4021 atctcgtagc gagcctgata ggcaagagaa actaaataaa aaaatgattc actggatgta
     4081 tggaattatc gcggtagcag tttgtttttt ccctataata tatgttgtgc ggttttatcc
     4141 gaggatcgcc acgctgtcct tcatagagaa catgaaatat tttgttctaa atttcaatgt
     4201 gacctttgct tattatgcca ttgcagtcaa tctgatttac cttgtgctat tgttcttttc
     4261 gaggctgaat attcgaaggg aggctaagct ctgggaactc aagaaggcca caatgttctt
     4321 caaaccccgc atgctgccct cggtctcgat catcgcacca gcctataatg aagaaaggac
     4381 catcatccag agtgcaaatt cactacttaa cctcagatat ccagaccatg agctagttat
     4441 cgtcaatgac ggatccaaag atgccactct caaaactctc atcgattact tcgatctcaa
     4501 aaggacagat tttccctatg agcagagact tccgcattac ccgatacgag gcatttatac
     4561 taacccctct ataccgaacc tggtagtagt cgataaagaa aatggcggta aggccgatac
     4621 tctcaatgca ggtattaatg tttcatctaa agaatacttc tgtggtatcg atgcagattc
     4681 gctcttggaa tcagatgctt tgctcaaggt tgcagcgctc actcttgatt atggtgttga
     4741 aactccagct ctaggcggca atgtattccc catcaatggc tgcaaagtcg acaaaggaaa
     4801 gatcgaaaga atcggtgtgc caaatcaatg gcttgcgcga cttcaaagtg ttgaatatat
     4861 gcgggccttt atgtgtggtc ggcttggatg ggattaccta aactctctcc ttataatctc
     4921 aggagctttc gggcttttta gaaaagaacg catcgtggaa gttggtggat acctgaccag
     4981 taaaagtcag taccagcgag atactgttgg cgaagatatg gaactcgtag ttcgcattgc
     5041 gcgcagcatg catgaagcaa agcgtcctta ccgcataagc tatgccttca atgctaactg
     5101 ttggacggaa gtaccagaaa aactttccag cttgaaaaaa cagcgatatc gctggcaacg
     5161 tggtctcgtc gacattatgc attttcataa gaagctgatt tttaacccaa aatatggcct
     5221 gatgggcatt ttggcaatgc cctactttct catatttgag atgattgggc ctcttttcga
     5281 aattcaaggc tatgttatgg tctttctagc cgcaatattt ggcatattga gcgcaaggat
     5341 agcgcttttg cttttctttg gttcggtgct tatgggcatt tttatctcca taatgtcgct
     5401 gaaaacagct gaaagacatg acatctacta tgactatcga gataccttca gattgatcgg
     5461 cacagcgatt gtagagaatt ttggtcctcg ccaactcttt tctacttggc gagccagtgg
     5521 ttttgtcaaa gcgatgcaga aacctcaagg ctggcaaaaa ttcgagcgga aaggctttgt
     5581 aggaaaggag ccttcagccg caagcacaga aggaattgtc catctatgat tacgcgaatt
     5641 acagaaagat ggcggactcg tcttctctgg atcattggaa tagcgctgct tgctttgtct
     5701 actttgctta tcgctttgcc ttttgtgctc tggaggtgcg aaccctatca tccgctcgaa
     5761 atatgggttg tcgataagac tgtaccttac acggatttta gggagcatgc agggcttttt
     5821 tggcttctcg acaacaacaa gatagcaaaa cctgacacaa agaagctcta taatgtacgc
     5881 actgattact atggctttta ccctgtatct aattctccag ctaattacga atggcgcagc
     5941 aattctctgc cggaaacaag taagaagcca gaccttatat atattgccga tacctatggt
     6001 gtgtatcgcg atgactatat gcagaaaaag tcacctgaag ataattcatc attgctttat
     6061 ggaggcctag atggcaatga ttgcaaaatc attcaaagcg cccttggtca aggcaataca
     6121 ttcattgccg aatttaacac cgcagcctcg ccaaccaatg ctaaaaatag agacctctta
     6181 ggccgcctca ttgggctctc gtggcgaggc tggatcggca attactatga tgaccttgct
     6241 ccccatggaa gcgtcccagc ctggattgtg cagaattggg aatccgcatt gaatcagaaa
     6301 tggacatttt ccggccgcgg aatcgtactt ctcaatgaaa ggggtgatat cgaagtactc
     6361 accgagaaag aggatataag ccctaaaggc atgaagatgg tcttcaatgc gccatgggac
     6421 agcgaaattg gcgtaaaaaa gccagtctca tatcgatatt ggttcgaatg gattactgcc
     6481 gaccctcttt tggaagaagt tgcgcactat gagctcaatc tcaagccaga gggcaagaaa
     6541 aaattggatc gattgaactt gcccaccact tttccggcca tagtccgcta taagaatgag
     6601 caatatacga gctggtattt tgcgggcgat tttgctgatc ttcaattctc gggaacacct
     6661 tatcgagtaa aaggcattca ggctataaag cgaatcctta cagatgaaac agtggaggat
     6721 aatagttgct ttttttggaa ggcctatgct ccaattatga actatattct cgaggaaaat
     6781 agaaaacaga gggcagagtt taatgccatc caaaagccta tcgaggttcg agtccctgtc
     6841 cgtgcgatca gagaaggctt tcagcttact tctccatcag gagattggaa tacaattttt
     6901 gtgagaagcg tcaatttagg gacagcggag cccggcaaat tttttacaga atttcctaat
     6961 gaaatcgata cctaccttcg atggctggcc atgattgccg atatgggggc caataccgtg
     7021 cgcatctaca cgctattgcc gcctgaattc tatcgtgcgt tagctatcta taatatgacc
     7081 cattccgaag cgacgctttt attgcttcaa gaaatctggc ctgaggaaca tcctcctgga
     7141 aatgacttct tgcagccatt gtatcaagaa gcctttaaaa aggagatcgg ttatgtgatc
     7201 gatgctgtct atggccgcgc aaatatacct gaaagaaagg gccgcgcctg gggtatatac
     7261 agcaccgatg tatctaaatg gttattgggt tggcttgtag gacgagaact cgaaagtgtg
     7321 gaagtactac agaccgatgc aaatcatcaa ggcattgctt atgcaggaaa gtttatctcc
     7381 gcaggcaaag cagcctcggc aacagaggtg tggctcgcag aatctctcga tgctgtctca
     7441 ataatcgaat atgagagcta tcaaaccctg caccctgtag gtattgttag ttggccaacg
     7501 ctcgatccta tacagcatga caccgaatgg gaccccataa caggaaagaa aaaccgttgg
     7561 aatgactgtt caagtatcca gatcgaacat ctcgaaacat cacctcttat gactgctggg
     7621 ctattcggtg cttatcatat ataccccaat tatccagatt tcattgtcaa tgaaccttcg
     7681 tataatctat acaaagactc tgtaggcatt ctgagatatg gcagatatct cgaagaattt
     7741 ataaagacac atgtgcgcta tcctgccata gttgccgaat ttggaatcgc gaatggtgca
     7801 ggggttgcgc atctctcgcc cgatgggctt catcatggag gcagcgatga agctactgct
     7861 ggcagggata tcctacgtat gtacgatgca atccaaaaaa caggctatac tggcgcttcg
     7921 atatttgaat ttatggatga atggacaaag aaaacctgga tacaagaacc attcatgata
     7981 ccattcgatc gcagagttct atggcacaat gcagtagacc cagagcagaa ttatggcctt
     8041 atagcgaatg aaagcatagc ccccactgcc ccagagaaga ccattcaagg cagtggcgcg
     8101 ctgatgcagc tagatattgc ccatgatgct acctacctct acctaaccgc aacgctttcg
     8161 gctgataatc ttattaacgg taatgaaata ctaatcggta tcgataccta taaaagggcg
     8221 gtggggcaga tggcttggcc aagagggctt ggaaccactg cctcaggcat ggaattcttg
     8281 ctagaaataa agccgcaaga gatgcgacta caggttattc catcctataa catcgccaga
     8341 gcgagatatg caagcacctt gatgcacagc ggagcattca gcgatatcga catgctagta
     8401 aacggcaagg tgatcaccaa ggatggaact atgataccag agaagcgctt caatgcttct
     8461 attcttcgcc agggcgaatt cgatgaatct ggaaatctct ggaatgtcca agggaatatt
     8521 ctgcgtgtgc gtatcccctg gaatcgcctc aatgttaccg acccttcatc gctgaaagtg
     8581 ctcgatgaca gccgctcgat cgataatccc gttaccgatc aactaaaaac tattgtcacc
     8641 gatgggtttg tgttcgatgc tcttgtctta gatagcacat cggggaaagt aataggcaaa
     8701 ctcgaagcaa acatagatgc gccatatatc tggtcgggct gggaagatac gccgccctat
     8761 cgcgagcgac tcaagaaaag ctatttcatc gtccgcgatt tgtggggagc tcaagcagaa
     8821 gaagaaatga caatacgtaa ggagttcatc gaagcgcaaa gtggcagatg atcctatgat
     8881 ctaacgccat agcatcctag gcaggataag tacgataaag aaggatgaac agcatgcatt
     8941 tttctgataa tatgattctc catgatgaaa agaatatttc atccaagcat tctatctgta
     9001 gcaatgattg caattgccgt tgatttagct ttttcttcgg ctctgaccaa acctattgca
     9061 aagaaggagt acagaaatgt gggatatgta ttccataaga attgtgacat caacacttta
     9121 gatgtaacca aaatcactca tctcaattat tcgttcggct tgatttacaa tagagaatat
     9181 gaagaattag gaaatgaaac aaaccccgat gtaattgtac ctcatgattt tgacgatagt
     9241 ctgctgcata caatctattt gtccgataag gttgaaagcg atctttcaag aatctatatt
     9301 ttgaaagaga agaaccctgt cttgaaaatt cttttatctg taggtggatg gggatgtaga
     9361 ggattctcag atgctgctgc atctaatgat gcaagaaaag cttttgcaca atcctgcagg
     9421 aagattatcg acagatttgg acttgatggg attgatttgg actgggaata tcctgtcata
     9481 gggggatggg gatcaataaa atctagaccc gaagataagc agaaccttac tttgttattg
     9541 aaagaagtga gaaaaacgat aggcaatgac aaattgttga caatagccgg ctcaccaagt
     9601 gttagtttct caaatggcga atggatagaa ttccaaaatg taatagatct gctagactac
     9661 ataaacatca tgacatatga ttttgggaac ggcacatgtt attttgattc ggcattatat
     9721 ccttctaaaa catggaaaac aaaatctgat cacgacgact ataatataga catggcagta
     9781 aaactatatt tgaagaatgg atgcccagca gagaaaataa atgttgggat ttcatttggg
     9841 gcgagcatac caaaagttgt agtatccaat aataagctat ggaaggctat atccaaaaag
     9901 ctaaatacgg tgggcttttt tacatctaaa gatcctctta taaaaaaagt aaaggatttg
     9961 ctagaagata agaatggctt ccataaagaa tgggacgaac aagccaagac aatgtatata
    10021 agtactgatc tattaaatgt cagacaattt gtcatgagtt acattgaacc ccaaggccta
    10081 tctgaaagaa ctaagtatat caaagacaat aacctaggag gaataatgtt ttgggaattt
    10141 ggtgctgatt atgacaactc tttagtttcg cagattgcaa gagaactcga tataaaccga
    10201 ttaggcgaaa a
//
LOCUS       DAOMZL010000061        10080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_6316_length_10080_cov_13.782444, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000061 DAOMZL010000000
VERSION     DAOMZL010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 10080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10080
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6316_length_10080_cov_13.782444"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PLQ30_05885"
     CDS             complement(<1..985)
                     /locus_tag="PLQ30_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPW02012.1"
                     /translation="MHNKVRFGIIGYGKVAALHAKALETTEHAELVSVSGHRQERRDA
                     FAHQWGLNSRNSVEEMIKEDGIQAVVIATPHLQHYPDAFSALNAGCHVLVEKPLALSV
                     AEAETLIIEANKRNLLLSVIAQRRWYPSCKRIRHAIDEGYLGTPALAQLTILGWRDEA
                     YYSSDPWRGSWEHEGGGVIVNQAPHQFDLLCWYMGEVAEVYGRWANINHPYIEVDDSA
                     VATVLFKSGGLASIFVSNSQRPGIYAKVHIHGSSGASAGVQTDGGAMFIAGRSSVLEA
                     PYNDIWTIPGQEECRAQWQKEDEEFFSHIDATWYFFAQQEEDFARAILEGQA"
     gene            complement(1084..2118)
                     /locus_tag="PLQ30_05890"
     CDS             complement(1084..2118)
                     /locus_tag="PLQ30_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPW02013.1"
                     /translation="MKAMILKEYKHLEIKDIPMPHIESADEVLIRVKATAICGSDIHG
                     FDGSTGRRQPPLVMGHEASGVVESVGTEVKTLKIGDRVTFDSTIWCGQCYFCRQGQVN
                     LCDARRVLGVSCDEYRQDGTFAEYVIVPERIVYRLPDNVAFEEAALTEPVSVAMHAFE
                     ITDMKPGESAAIVGTGLIGLLLIQIIRAYSPHLILAFDTDEKRRKAAIEAGADVALDP
                     QDPDVHSTVLSMTNDRGVDRAFEAVGASAAIGTAIESVRKGGSVTLIGNISSKAEIPL
                     QSVVTRQISLLGSCAIAGEYPISLALMANRNINVRQVISNIVPLEEGPLWFDRLYKRE
                     PGLLKIVLTP"
     gene            complement(2148..3233)
                     /locus_tag="PLQ30_05895"
     CDS             complement(2148..3233)
                     /locus_tag="PLQ30_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HPW02014.1"
                     /translation="MNPIRLLPEEDVKLDLLSLGGLVMRMDPGIVPFAFAENYSVHVS
                     GGEYNVAANLARCFGKRTAIATAIVDNPLGEKIAAEVRKMNVLGIYKHFKSDGVRGPN
                     MALVWSDRGHGVRPPVVFYNRSNEAAALLKPSDFDFEPIFKEGVRWFHSGGIFAALSD
                     STPQLIIEAMKIAHDHGALVSFDLNYRAKLWAAANTGEKHSDVFGRIVEHVDVLLGNE
                     EDLQMGLGLPGPDIHAVSKLDPSSFLKTIEQVHQRWPNIKAVATTLRDVKSTNRHLWS
                     AVLWIEGNHWVAPTMELDIYDRVGGGDGFASGLIYGLLEGEDPEEALRLGWAHGALLT
                     TYPGDTTMASLEQVKALARGQSARIQR"
     gene            complement(3284..4735)
                     /gene="gnd"
                     /locus_tag="PLQ30_05900"
     CDS             complement(3284..4735)
                     /gene="gnd"
                     /locus_tag="PLQ30_05900"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HPW02015.1"
                     /translation="MKADIGLIGLAVMGENLVLNMESHEFTVAVYNRTVEKVDSFLKE
                     RGAGKRILGAHSPKELVEMLKKPRIVMLMIRAGTAVDEMIAQLIPFLEPGDIIVDGGN
                     SNYEDTMRRFSELESKGFLFVGTGISGGEEGALTGPSIMPGGSEAAWPHVKPIFQAIA
                     AKVDNQSPCCEWVGPSGSGHFVKMVHNGIEYGDMQLISEVYQIMRDRLSMSPDEMAMV
                     FDEWNKGELDSYLTSITSDILRYKDEDGTPLIEKILDAAGQKGTGKWAGINALNFAVP
                     LTLIVEAVFARCISAMKDERLRAVKVLGRPTGTEYSGSHEKALKDLGAALYAAKIISY
                     AQGFMLMREASTVYDWKLNYGSIALMWRGGCIIRSRFLGRIKEAFDVDPGLSNLVFAP
                     FFAERLLKNEKAIRNVVTEAVAGGIPVPALSSALAWFDALRTERLPANLIQAQRDYFG
                     AHTYERIDKPRGQFFHTNWTGHGGSTSASTYIV"
     gene            complement(4752..5603)
                     /locus_tag="PLQ30_05905"
     CDS             complement(4752..5603)
                     /locus_tag="PLQ30_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HPW02016.1"
                     /translation="MERSAAPSCLYLIHSLPNSLSERDRRIADYILNDPAKAVHLSIE
                     ELAEAAQVSVSTLVRFVKKLGFRGYQQFRIALASEALAPEARIYETIVDSGEDPVRLA
                     FSSASKALEITSSMIDSQDLYKLASRIIEANCVYLFGLGGSLIVAKDALHKLVRTGIR
                     CIGAEDYHMQLMIASQMNQQDTAIIVSHTGANKDAIGLAETIKTTGAFLCVITTYPRS
                     ALSRMADMRFISASSGSQAISEAFSARIAQLALIDSLYIAIMKQLGEEGIKNVEKMRA
                     TIAKRRI"
     gene            5827..7077
                     /locus_tag="PLQ30_05910"
     CDS             5827..7077
                     /locus_tag="PLQ30_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPW02017.1"
                     /translation="MKRKLMLVLVALLALFAVSAQQKEIRVLLANHPYGDLLKSAIPE
                     YEKATGVKVNVESLQESQLTTKLTTEFATRSSTVDVFMTRPLQEGKMFYKNGWYEPIT
                     GYDFSDYPKNAMDVASFGNKAYIVPLVTEWEVLYYRIDLLKKAGITVPTNFAELEAAA
                     KKLHSGDIVGFASRGKGAAAVTQLSSYVYNYGGAYLDKGKAVFDSKEALEAIRFYGRM
                     LANYGPAGVTNMSWENIMPLFQAGKIAMWTDASVFYGQIVDPSKSQVPAENVGIANFP
                     AGPKGNTPFIVVSWGMSIAKQSKNKELAKDFIKWATSKDLAKRGMLANITMARGSVWK
                     DAEVRAKIHPSLIATQEYAAKYGIPYDRPYMSAVGEARDLIGEVVIESINTAGTSPKL
                     EALAKEKAAAVNALLEDTGEYGVY"
     gene            7140..8030
                     /locus_tag="PLQ30_05915"
     CDS             7140..8030
                     /locus_tag="PLQ30_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPW02018.1"
                     /translation="MVSKRNRGSFIEKNLHIIFPLPAVVFIIVMMLFPVLYTFFMSFT
                     NWNLTSGMPLSFVFLSSYKRVLQEPRFIKALGRTFSFTALAVSIETVFGMVIALILNR
                     EFKAKGFTKLIMLLPLVSTPVAIGIAWNLFYDPTIGLMNFMLTQLGLPKLAWTGSAST
                     VIPSLALVDIWQWTPMISLIMLSGLASLSNEPYESARVDGATEWQIFWHITLPMVMPT
                     LLTAIILRTIDALKTFDIIYAMTGGGPGYASETLNIMAFKYSFEYFRMGQASVVLIIL
                     FLLVLAMSLLIVRMRKSLEL"
     gene            8050..>8495
                     /locus_tag="PLQ30_05920"
     CDS             8050..>8495
                     /locus_tag="PLQ30_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPW02019.1"
                     /translation="MKRYIRKILFFILIIAIVVPLLFPFVWMLSSSFKTQVDIVAWPP
                     KLIFSPTLQNYKRVFSEQNFLKYFVNSSIIGISSVFFSLLMGLPAAFSIARFGQKKLS
                     IFILIARLMPGISFLMPWYIIFSRLRLMDSYVALILSHMLITLPIIV"
     assembly_gap    8496..8595
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8964..9833
                     /locus_tag="PLQ30_05925"
     CDS             8964..9833
                     /locus_tag="PLQ30_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPW02020.1"
                     /translation="MQESSKKLGYTEGIASIIINTMLFGLKIWVGTASSSVAMTADAW
                     HTLSDTLTSIVVIFGFWFSSRPEDEKHPFGHGRAELIATIIIATLLAVVGLNFLRDSI
                     QQLIVRKSANFSTLGLVVFAVSVVIKEGLARFSIWAGKKTNSQSLLADGWHHRSDAIA
                     SLIIVIGAVLGKYLWWIDGVLGIGVSGLVLYAAYDIAHTASDSLMGEKTSDDLTKRIK
                     RIASEASPELKDIHHIHIHRYGDHLEITLHARLDKKTDIEDAHALSTRLEQRLRNELR
                     ADTTVHIEPVKEA"
BASE COUNT         2591 a         2371 c         2228 g         2790 t
ORIGIN      
        1 gagcttgccc ctccaaaatg gcgcgagcaa aatcttcttc ttgctgtgcg aagaaatacc
       61 atgttgcatc gatgtgtgaa aaaaattcct cgtcttcttt ttgccactgt gcacggcact
      121 cttcctgtcc aggaatggtc caaatatcgt tatatggtgc ttctagtacg ctactgcgac
      181 cggcaatgaa catcgctcca ccatcggttt gaactcctgc agaggcgccc gaactgccat
      241 gaatatggac tttcgcataa attcctggcc tttgtgagtt tgaaacaaat atcgatgcaa
      301 gacctccaga tttaaaaaga accgttgcaa ctgccgaatc atcgacctct atgtaaggat
      361 gattgatatt cgcccatcta ccgtagactt ccgccacctc tcccatatac caacataaaa
      421 gatcgaactg atggggggcc tggttgacaa ttactccgcc gccttcatgt tcccaggagc
      481 ctcgccatgg atcagagcta taataagcct catctcgcca gcccaggatc gtgagctggg
      541 caagtgccgg ggtgccgaga tatccctcgt ctattgcatg acgaatacgc ttgcaggatg
      601 gataccagcg ccgctgcgct ataacactga gaagcagatt tcgcttattt gcctcaataa
      661 tcaatgtctc ggcttcagct accgagagcg caagtggttt ttctacaagc acatggcagc
      721 ctgcattgag cgctgaaaat gcatctggat agtgctggag atggggtgtt gcaatcacca
      781 cagcttgaat gccgtcttct tttatcatct cttctacact attgcgagaa tttaaccccc
      841 attgatgggc aaaagcatct cgcctttcct gtctatgacc gctcacagat acaagctcag
      901 catgctcagt tgtttccagt gcctttgcat ggagtgccgc gacttttcca tatccgatta
      961 ttccaaatcg aaccttatta tgcatgtctt tctcctatgg atgggggaac cctatatttg
     1021 agaatatgtt atgaaacccg cctaatgcgg tcaacaagaa taacgcggaa ttcgatccac
     1081 tcatcaagga gtcaatacaa tctttagaag cccgggttct cttttatata gtctatcaaa
     1141 ccacaacgga ccttcttcga gaggaacaat gttgcttatt acctgcctga cgttgatgtt
     1201 tcgattcgcc atcaatgcaa gggaaattgg atactcgcca gcaatcgcac aagaacccag
     1261 caaggaaatt tggcgtgtca caaccgactg caatggaatt tcggccttgc tgctgatatt
     1321 gccaattagg gtcaccgaac ctccctttcg tacactttct atagccgtac caatagcagc
     1381 gcttgcacca accgcctcaa acgctcggtc gacaccccta tcgtttgtca ttgaaagcac
     1441 cgtgctatgg acatccggat cttgagggtc gagcgcgaca tcggctccag cctctatggc
     1501 cgctttcctt cgcttttcat ccgtgtcgaa cgctagtatt aggtggggcg aatatgcgcg
     1561 gattatctga ataagcaaca gtccaataag ccctgtgcct acaattgccg cactttctcc
     1621 tggtttcatg tctgtgattt cgaacgcgtg catagcaaca ctgacgggct ccgtgagtgc
     1681 ggcttcctca aatgctacat tatccggcaa tctatacaca attctttcag gaacaattac
     1741 atattctgca aatgtgccat cctgtcggta ttcatcacag gagactccaa gtacgcgcct
     1801 tgcgtcgcac agatttacct gcccctgtct gcaaaagtag cattggccac accaaattgt
     1861 cgaatcaaag gttactctat ctcctatctt gagtgtctta acctcagtcc ccactgattc
     1921 aactacgccg gaagcttcat gtcccatgac aagcggaggc tgtctccttc cggttgaacc
     1981 gtcaaaacca tgaatgtcag aaccacaaat tgccgttgcc tttacccgaa tgagcacctc
     2041 atcggccgat tcgatatgtg gcatcggaat atcttttatt tccaggtgct tgtattcttt
     2101 caatatcatt gccttcatag ctgtcacttc ttaccaatgc gattccgcta tctctgaatg
     2161 cgcgcggatt ggccacgtgc aagtgccttg acttgctcga gacttgccat agtcgtatcg
     2221 ccaggataag tagtcaaaag cgcaccatga gcccatccca gtcgaagtgc ttcttctgga
     2281 tcttcccctt ccagcagtcc ataaattaga ccagaagcaa acccatctcc gccgcccacg
     2341 cgatcgtaga tatcgagctc cattgtagga gcaacccaat ggtttccttc tatccagagc
     2401 actgcgctcc aaagatgccg attagtcgat ttgacatcgc gcaatgttgt tgcgacagcc
     2461 tttatattag gccatctttg gtgaacttgc tcgatggtct taaggaagga agagggatcg
     2521 agcttggata cggcatgtat atctggtccc ggcaaaccca gtcccatctg taaatcttct
     2581 tcattgccca aaagaacatc gacatgttcc acgattctac caaacacgtc tgagtgtttt
     2641 tcgcctgtat tcgccgcagc ccagagctta gctcggtaat tgaggtcgaa agatactagg
     2701 gctccatggt cgtgagctat tttcatagct tcgataatca gctgaggagt tgagtcggat
     2761 aaagctgcga atatgcctcc agaatggaac caccttacgc cttctttgaa gattggctca
     2821 aaatcgaaat cgctcggttt caataaggct gctgcttcat tggaacgatt gtagaatact
     2881 acaggtggac ggactccgtg acctcgatct gaccatacaa gagccatgtt tgggcctcgc
     2941 actccgtcgg atttgaaatg cttatatata cctaggacgt tcatttttcg gacttctgcg
     3001 gcgatcttct cccccaatgg attatcgact atagcagttg caattgcggt tctctttcca
     3061 aagcatcgcg ccagattggc agctacgtta tattctccgc cagagacatg gacactataa
     3121 ttttcggcaa aggcaaaagg cacgatgcca ggatccatcc tcatgacaag gcctcccagg
     3181 gatagcaggt ccagcttcac atcctcttcc ggaagcaatc tgattggatt catgctttca
     3241 atctcctcat attgaataat tatgtctcgt aaatacgcga atatcagact atatatgtgc
     3301 tcgcagatgt agaacctcca tgacccgtcc aatttgtatg aaagaactga ccgcgaggtt
     3361 tgtctattcg ctcataagtg tgtgcaccga aataatctcg ttgtgcttga attagatttg
     3421 cgggcagtct ctcggttcta agcgcgtcga accaagcgag ggcagaagat agagctggta
     3481 ccggtattcc tccagccaca gcctctgtta ccacatttct tatggccttt tcattcttga
     3541 gaagccgttc ggcaaagaaa ggagcaaaca ctagattgct cagccccgga tctacatcga
     3601 aggcttcttt tattctacca aggaatcgcg aacgtatgat gcaaccacct ctccacataa
     3661 gagcaattga gccataattt agtttccaat catatactgt cgaagcctca cgcatcagca
     3721 taaagccctg agcatatgag atgattttcg ccgcatatag cgctgcaccc aagtccttta
     3781 aggctttttc atgtgagcct gagtattctg taccggtcgg tcttcccagt acttttaccg
     3841 ctctaagtcg ctcatccttc atagcagaga tacagcgggc aaatacggct tcgacgatca
     3901 aagtaagcgg tacagcaaaa ttgagagcgt tgattccagc ccactttccc gttccctttt
     3961 gtcctgctgc atcgagtatc ttttctatta gaggggtccc atcttcatct ttatatcgaa
     4021 gaatatcgct tgtaatagaa gttagataac tatcgagctc tcctttgttc cattcgtcga
     4081 agaccatcgc catttcatcg ggactcatgc ttagtctatc tcgcataatt tggtagacct
     4141 cagatatgag ctgcatgtct ccatattcga taccattatg gaccattttg acgaagtgcc
     4201 cagatccgct tggtccaacc cattcacaac aagggctttg gttatccact tttgctgcaa
     4261 tcgcctggaa aattggctta acatgaggcc atgctgcttc ggaacctccc ggcatgatcg
     4321 aagggccggt aagagctccc tcttcacctc cagagattcc ggtaccaaca aataagaagc
     4381 cttttgattc aagctcggaa aagcgcctca tcgtatcctc ataatttgag tttcctccat
     4441 ctacgatgat gtcaccaggc tccaggaagg gtatcaactg tgcgatcatc tcatcgacag
     4501 ccgtgccagc tcgtatcatt agcataacaa ttcttggttt cttaagcatt tctacgagct
     4561 cttttggaga atgggcgcca agaattcgct ttcccgctcc tctttccttt aagaaagaat
     4621 ccactttttc aactgttcgg ttgtagactg ctacagtgaa ctcgtggctt tccatattca
     4681 gtacaaggtt ttcgcccatc accgctagtc caatcaatcc tatgtcggct ttcataaatc
     4741 ctcctctatg atcagatacg acgctttgcg atggtagcgc gcattttttc cacattcttt
     4801 atgccttctt ctcccaactg cttcattatt gcgatataga gcgaatcgat tagcgcgagc
     4861 tgcgcgattc tggcagaaaa ggcttccgat atggcttgag accccgaaga ggccgatatg
     4921 aagcgcatat cagccatcct ggatagagcg gaccgtggat aagtggtaat cacgcacaga
     4981 aaagcccccg ttgtcttgat cgtctcggca agtccgatgg cgtccttatt agccccggta
     5041 tgggatacta tgatggctgt atcctgttgg ttcatctgtg aagctatcat gagctgcata
     5101 tgataatcct cggcacctat gcagcggatt cctgtgcgaa caagcttatg caatgcatct
     5161 tttgcaacta tcaaagatcc acctaatcca aaaaggtaga cacagttggc ttcgatgatt
     5221 cgcgaagcca atttatacaa atcctggctg tctatcatag atgaagtgat ttcgagcgcc
     5281 ttcgaggctg agctgaaagc caagcgaacc ggatcttctc cgctatcgac tatggtttcg
     5341 taaattcttg cctctggtgc cagagcttca gaagccaaag ctattctgaa ttgctgatag
     5401 cctctaaaac ctagcttttt tacgaagcgt accagtgtag agaccgaaac ctgggcagct
     5461 tccgctaact cctcgatgct gagatgcact gctttggctg gatcattcag aatataatct
     5521 gctatgcgtc tatctcgctc gctcagtgag ttcgggagcg agtggatcag atataaacag
     5581 ctcggcgcag cggatctttc catagaaata ccatcaagtt tccctatctt atattagatt
     5641 ctcggataag tacagaggac tggcaaaaat ttccattcac ttgaaaaaaa attaccttca
     5701 tgtgttgaca aaatgctctt ttggctctta gtattaaagt tgtcaaattc gcctggcctg
     5761 aaacaagctc agactggagg gcggattatt atcgaattgc aacacatttc tatttaggag
     5821 gtcatgatga aaagaaaact catgcttgtc ttagtggctc tgcttgctct atttgctgta
     5881 agcgctcaac aaaaggagat ccgagtcttg ctggcaaacc acccctatgg cgatctgctt
     5941 aaatcggcca ttccggaata tgagaaagca acgggagtaa aggtcaatgt ggaaagcctt
     6001 caagagagtc agctcacaac aaaactgaca acggaattcg cgaccaggtc ttccactgtg
     6061 gatgtcttta tgacgagacc tctacaagaa ggaaagatgt tctataagaa tggctggtat
     6121 gagccaataa ccggatatga tttctccgat tatccgaaaa atgcgatgga tgtagcttct
     6181 tttggtaaca aagcttacat agttcctctg gtcaccgagt gggaagtgct ttactatcgc
     6241 atagatctac tcaagaaagc cggcattaca gttccaacaa actttgccga actggaggcg
     6301 gcggcgaaaa agctacattc tggagatatc gtaggttttg catcgagagg gaaaggtgct
     6361 gccgcagtga cccaactctc gagctatgtg tacaactatg gcggagcgta tctggacaaa
     6421 ggcaaagcag ttttcgacag caaggaagcc ctggaggcta tccgtttcta tgggagaatg
     6481 ctggccaatt atgggccggc cggtgtaacc aatatgtcct gggaaaatat catgccactt
     6541 ttccaggctg gaaaaattgc aatgtggaca gacgcttctg tcttctatgg ccagatcgta
     6601 gatccctcta aaagccaagt acccgcggag aatgtaggca tagccaactt tccagcagga
     6661 ccgaaaggca acacaccctt cattgtggtt tcctggggta tgagtatcgc caagcagtca
     6721 aaaaacaaag agttggccaa agacttcatc aagtgggcga caagcaagga tctcgcaaaa
     6781 cgtggcatgc tagctaacat aaccatggct cgcggttctg tgtggaagga tgcagaggtt
     6841 cgagccaaga tccatcccag ccttatagct acacaagaat atgctgcgaa atacggtatt
     6901 ccgtatgatc gcccctatat gagtgcggtg ggcgaagctc gtgatctaat aggagaagtc
     6961 gtaatcgaat cgatcaacac ggccggcaca tcgcccaagc tcgaagcact ggcaaaagaa
     7021 aaagctgccg cagtaaatgc tctcctggaa gacaccggag aatatggtgt gtattgacga
     7081 gcgatcagaa actgttttct gatagagcta ttattttagt ccccgaggtg aaatagatca
     7141 tggtttctaa gcgaaataga ggaagcttca tcgagaagaa tctacatata atcttcccgc
     7201 ttcccgctgt ggttttcatc atagtaatga tgctgtttcc cgtactctac acgtttttta
     7261 tgagttttac gaactggaat ctcacctcgg ggatgccact ctcctttgtt tttttgagct
     7321 cctataaaag agtcctgcaa gagccgcgat ttatcaaggc tcttggcagg actttttcct
     7381 ttacggctct ggctgtctcc atcgagacgg tgtttggcat ggtgattgct ctcattctaa
     7441 accgcgaatt caaggcaaaa ggttttacaa agctaatcat gcttctgcct ctcgtcagta
     7501 ctcctgttgc tattggtatt gcatggaacc tcttttatga tccaaccata gggcttatga
     7561 acttcatgct tactcagctg ggcttgccga agctggcatg gacaggatct gcctcaacgg
     7621 ttattccatc tctggcgttg gtcgatatct ggcaatggac accgatgata tctcttatca
     7681 tgctctcggg gctagcaagc ttgtctaatg agccttatga atcagcacga gtcgatggag
     7741 ccacggaatg gcagatattt tggcacataa ctctgcctat ggttatgccc actcttttga
     7801 cagccattat tttgcgcaca atcgatgcgc tcaagacttt cgatatcatc tatgctatga
     7861 caggtggagg gccgggctat gcttctgaga cgctcaatat catggcgttc aagtatagtt
     7921 ttgagtattt tcgaatggga caagcttcag tagtgttaat aattctcttc ttattggtgc
     7981 ttgctatgag cctcttgatt gttagaatgc ggaaatcttt ggaactgtaa tgatagaacg
     8041 aggttttgga tgaaaagata tataagaaag atattatttt ttattctaat cattgcgatt
     8101 gttgttccgc tgctttttcc ttttgtatgg atgctctctt cttcattcaa gactcaggta
     8161 gacatcgtgg catggccgcc aaagttgata ttcagtccga cattgcaaaa ttacaaacgg
     8221 gtttttagcg aacagaattt tcttaaatac ttcgtcaatt cgtcaattat agggatttcc
     8281 tcggtattct tttctcttct tatgggattg ccagccgcat tctcaattgc caggtttggt
     8341 cagaaaaagc tttcgatctt tattcttata gcgcgcctca tgccgggaat atctttcttg
     8401 atgccctggt atataatctt ctcgagatta aggctcatgg acagctatgt tgctctcatc
     8461 ctttcgcata tgttgataac tcttccgatt atcgtnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnntccta tgcggaagtc gattggggtg cagtaatggc cgccgcagtg
     8641 gcaattatgg ctcctgcaat tattctgacc atgattttcc agaaatatgt ggtgcaaggc
     8701 ctaacaatgg gtgcggtaaa gggataagcc agacagccag cctattatag ctatagactc
     8761 tttgaatcct atgtgtattt tgctacttga cctagataga ccggtggcat atacatgcaa
     8821 taatagatgg atcaggtttc tcttgtcgaa aagagttgac cgggcttttg cgcagcggga
     8881 cattattcct gcgggaccaa tggaaatatc tgcggatatg cgaaaacgat atagcagatg
     8941 tctttcaatt ttggaggttc tacatgcagg aatcatcaaa gaagctcggt tataccgagg
     9001 gaatagcatc aataatcatc aatacaatgc tttttgggct caagatatgg gttggcacgg
     9061 cttcgtctag cgtagccatg acggctgatg catggcatac tctatcggat actcttacct
     9121 ccatagtagt gatatttggt ttttggttct ccagccgtcc tgaagacgaa aagcatcctt
     9181 ttggccatgg aagagctgaa ctcattgcta ctataataat tgctactctt ttagcagttg
     9241 ttggcctgaa ctttcttaga gattcaattc agcagttgat agtccgaaaa agcgcgaatt
     9301 ttagtacctt aggtcttgtg gtgtttgcgg tctctgttgt aattaaggaa gggcttgcga
     9361 gattctcgat ttgggctgga aagaaaacaa atagccaatc tctcctcgcc gatggctggc
     9421 atcataggag cgatgcaatc gcctctttga tcattgttat cggagcagtg ctagggaaat
     9481 atctatggtg gattgatgga gtcctgggca taggtgtatc aggcctggtt ctctatgcag
     9541 cttatgacat cgctcacact gcttctgatt ctcttatggg tgagaagact agcgatgatt
     9601 tgactaagag aatcaagaga attgcaagtg aggccagccc ggaacttaag gatattcatc
     9661 atatacacat ccataggtac ggagatcatt tagagataac tctgcatgca aggctcgata
     9721 agaaaaccga tatagaagat gcacatgcgc tttctacaag gcttgagcag agactaagaa
     9781 atgaactcag agcagatacg accgtgcata tcgagccggt aaaagaagct tgaagagcta
     9841 cggcaaatgg tccaggcttt tcacagtttc cttgaatgtc taataatgga agaaaggctg
     9901 tctaagcatt agtctgctat agagcataga gacttttttg ctcaaaatcg ttataataat
     9961 aatagagctt catgccacga gcgtaatggc gagaaagcga aaaataagat agagatagct
    10021 tttggagcac ttgacaacta tcgatagatt tggtagtttc atcgagcgct ctttaggtta
//
LOCUS       DAOMZL010000062         9958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_6431_length_9958_cov_13.678885, whole genome shotgun sequence.
ACCESSION   DAOMZL010000062 DAOMZL010000000
VERSION     DAOMZL010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 9958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9958
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6431_length_9958_cov_13.678885"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="PLQ30_05930"
     CDS             complement(<1..1121)
                     /locus_tag="PLQ30_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02021.1"
                     /translation="MISARLAWRNICLHRKKSLIIGLIMGFGILILFVGNSLIDTTIS
                     GLRRMFVEGYTGDLMVTGPTEFSTTVLGETAGSEEVLPHIAQIKKYEDFLEARQDVAS
                     ILPILSGQVSFGIGEEELGSGYCFGVNIFDYRAFFPDNLTLVSGEWPEESDEPWVLLS
                     EPTYALLQQSSDIEVKPGTKIIMTALANSAGTVIREVSVKGIIKFNQSNAQLSGITLV
                     DADTMRDLLGFASLRDGAPQLSEHEAAFLTDFDPDSLFDNLFASQTITTEGEGQSELG
                     LEPSQLDIPEDPGQEISQASSTTTVIAEIVPAWQFLLIRAKNGSNTSKLLDDLEAFSE
                     ELGDGDRVQDWIAGAGKVARTAVTIRLVFDLLVAVIAVVV"
     gene            complement(1241..1936)
                     /locus_tag="PLQ30_05935"
                     /pseudo
     CDS             complement(1241..1936)
                     /locus_tag="PLQ30_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271504.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2131..2736
                     /locus_tag="PLQ30_05940"
     CDS             2131..2736
                     /locus_tag="PLQ30_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HPW02022.1"
                     /translation="MVKRCLVCITGASGAIYSIRLLCALAANGVRIHVVTSERGARVL
                     LEETGRPLGYWLGRLQSQGGPEGRHAFITMHENNDFSAPISSGSFRLHGTVIVPCSMG
                     TLSTIASGNCSNLIHRAGAVALKEGWPFIVVPRETPLSLISLRAMTQLKEAGAVILPA
                     SPSFCGKPPDIQSLVDTVVYRILDHLDIVDKAMTGEDPGKS"
     gene            complement(2816..3937)
                     /locus_tag="PLQ30_05945"
     CDS             complement(2816..3937)
                     /locus_tag="PLQ30_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW02023.1"
                     /translation="MTAKSVPVALSHVTKIFKDPKGKGEVHAVEDADFEVKPGELVTL
                     LGPSGCGKTTTLRMIGGFELPTKGKIIIGDTDVTFLPPNGRQTATVFQSYGLFPHMDV
                     FDNVAYGLKIRKLPKNEIEKQVTDILDLVGLSELMHRAPGRLSGGQQQRVALARSLVV
                     EPQVLLLDEPLSNLDALLREQMRVEIRRIQKTLGITAIYVTHDRIEAMSLSDRIIVMR
                     EGKILQIGTPSEIYVEPDSVFVAGFVGKVAFFHAQLEGFEPDGRAICRLGQKMVHASS
                     AVKGLKPGDDVELMARPESLRIVSQEEAVASGQVFAKVYLGSNVEIYVKTETGELLIE
                     IEDPLGKVIPSEGETVWIGFNERKVRALPTEEQAEEQSK"
     gene            complement(4000..6204)
                     /locus_tag="PLQ30_05950"
     CDS             complement(4000..6204)
                     /locus_tag="PLQ30_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPW02024.1"
                     /translation="MFTKKKIIMLSVVCLIFALGEYWIFSTIKNTFEKNTLKEMKALV
                     KAMTSFAPSNNEALSDWIEELGAIYSDNRFFVIKGVPGEEGSEELGGDDALLELWQLP
                     EYADTASSAAESVSYLEIYKWPKIVHFKGIPYTLMVAPIPDAENSEARATLLAAIDST
                     GYVSFTRLVDALALIFFALFAIGFGIATFSRDPISGYAIVVLAAIVLAFVAYPLLEAV
                     RLTLIKDGRFSFNVWKNILSNRQYLQALWGSIQLGIVTATISTFIGFVYAFAVARTSI
                     KGKKLITTLATMPVISPPFSLTLSIILLFGKNGIVTRQWLGLQNVNIYGLWGLALAQT
                     ISMFPIAFMTMQGVLEAIDSTLEDASLDLNASRWYTFSHVTLPLAAPGLLSSWLLVFT
                     NSLADFANPLLLSGSYRVLSVESYIEAIGMNRLNNGAALSLLLLLPTLTAFLAQRRWV
                     NRKSFVVVTGKPSTRLSDLASSGVRKALTGFVILVSAFIVGLYGTIVAGCFVKNWGID
                     YSFTLANIGEALTRGKQSLVSTITLAGIATPIAGILAMVAAFILVRKNFPGKRILESL
                     IMAPFAIPGTLIGISFILAFNKPPLLLVGTGAILVINYVVRELPVGVEGGIAALRQID
                     PSIEEAAADLGADQATIFRTIILPLIRPAFISSMSYTFVRSMTAVSAIIFLISARWYH
                     ITIQIYNFSENLRFGLASVLATTLIIIVLAVFGLMRLLVRQSEYLEKTITTQ"
     gene            complement(6343..7338)
                     /locus_tag="PLQ30_05955"
     CDS             complement(6343..7338)
                     /locus_tag="PLQ30_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW02025.1"
                     /translation="MKRLMIAGLVLILSLTVAFGQQRFGVYTTLDEPLAKALFDEYQK
                     ETGIMLNWQRLSGGEVEARLEAEKANPQASIWVGGVGLNHISAKLKGLTAPYKSKMLE
                     NTPLQFRDPENYWVGLYIGPICFVTNNKVAKEQGIKPPTSWADLLKPEYKGKIRVANP
                     TTSGTAYNVITTLRYVFGGDEEKVFDYLNKLDKNIDQYTKSGAAPGKSVAIGEIPVAI
                     GYAHDNVKLKVEGADVTITFPSEGTGYEIASMSIVKGGPDPVNAKKLYDWVLSAKAQE
                     IIASWYVIPVSKIAKKNPQAFSLSEIKTVDQDVMWDAANKDRLLERWVKEIGSKR"
     gene            7776..8195
                     /locus_tag="PLQ30_05960"
     CDS             7776..8195
                     /locus_tag="PLQ30_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPW02026.1"
                     /translation="MNNLVLYDKSPFDLIESLFNNDDWLMPSFRTPAIDVYEEGDKYT
                     IEADLPGLTEKDIKLEVRNGQLILSTAKREKSEDKSKKGRWIRRERREYQFQRSFTLP
                     EEVDPEKIEAHFKNGVLQVVLPKKPEAAPKSIEVKIN"
     gene            8368..8598
                     /locus_tag="PLQ30_05965"
     CDS             8368..8598
                     /locus_tag="PLQ30_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPW02027.1"
                     /translation="MADKSNRLPQNAKGKWYVDASCIGCGLCSVTAPDIFILGDDGQA
                     YVARQPQTEEENELAIQAMNDCSAQSIGDDGE"
     gene            complement(8614..9732)
                     /locus_tag="PLQ30_05970"
     CDS             complement(8614..9732)
                     /locus_tag="PLQ30_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPW02028.1"
                     /translation="MEDQRLRRLAQLLVNYSTRVQNGDKVLIENSNLEPDFTRLLIEE
                     VHRAGALAFISLRDRRIERTLFMDAPDEQFELQAEFELARMDKMDVYIGFTSVRNSFA
                     WQDLPASKIELYNNHVWKKVHIDRRIPHTRWVVLRYPSAAMAQNAGMSEDAFEKFYFD
                     VCTMDYSRMSRAMDPLVELLQKSDRVKILGHDTDLEFSVKGLPAIKCDGTMNIPDGEV
                     YTAPVRDSVNGILSYNTPNEKDGFKFENIVFEFKDGKIVRARANDTERINKILDIDEG
                     ARYLGEFSFGINPYITRPILDTLFDEKIAGSLHITPGNSYDDCFNGNRSALHWDIVLL
                     QDEAAGGGEVWIDGKLIRKDGLFTLPELRGLNPEALKG"
BASE COUNT         2709 a         2418 c         2151 g         2680 t
ORIGIN      
        1 acgacgactg caatgaccgc aaccaataga tcgaatacaa gccgaatcgt taccgccgtc
       61 ctagcgacct ttccggctcc tgcaatccaa tcctgcacac gatctccatc tccaagctct
      121 tcggaaaaag cttcaaggtc gtctaaaagc ttggaagtgt tagaaccatt ttttgcgcgg
      181 ataagaagaa actgccaggc aggtacaatc tctgcgatga cggtggtcgt gcttgaagcc
      241 tgagatatct cttggccagg atcttcagga atatccaatt gcgaaggctc caaacctagc
      301 tcggattgcc cttcgccttc tgtcgtaatt gtctggctcg caaacagatt atcgaatagg
      361 ctatcaggat cgaagtcagt caagaaggct gcttcatgct cactcaactg aggagctcca
      421 tcgcgcagac ttgcaaaacc caagagatca cgcattgtgt ctgcatcgac aagggtgatg
      481 ccggaaagct gcgcattgct ctgattgaat ttaataattc ccttcacact gacttcgcgt
      541 attactgtgc ccgctgaatt cgccaatgca gtcatgatga tctttgtccc gggcttgact
      601 tcgatatcgc tgctctgttg aagtaatgca tatgtgggct cggatagaag aacccatggt
      661 tcatcgcttt cttcaggcca ctctcccgac acaagagtca ggttatcagg aaaaaaagcc
      721 cgataatcaa agatattcac tccaaaacag taacccgatc cgagttcttc ctcaccaatt
      781 ccaaaggata cttgcccact taggatcggg agaatcgagg caacatcctg gcgtgcttcc
      841 aaaaaatctt cgtatttctt tatttgagca atatgaggca gcacttcttc gctgcctgcg
      901 gtttcgccaa gcacggttgt gctaaattct gttggaccag ttaccatcag gtcgccggtg
      961 taaccttcca caaacatgcg gcgcaggccg gatatcgtcg tatcgatcag agaattccca
     1021 acaaatagta ttagaattcc aaatcccatt attagaccta taatgaggga tttttttcgg
     1081 tgaaggcaaa tattcctcca tgcaagacgt gcagaaatca tgcttgactg ccttctgtgc
     1141 cagtggcttt tgtgccagta agtcctgtgc cagtcgcttc tgggctagtg gctctaaccg
     1201 aaggacgaaa acccaattta tcatcccgat cttcatctat tctgcgatcc tctacgatta
     1261 tgccatcttt gagccttata atgtgatcgg cttcttcgac tatgtcagga tcatgggtgc
     1321 tgaagatgaa ggtcgtgcca aatgtttcat tcatcttgcg cattaggcgc aggatgcttt
     1381 cgcctgtagc agagtccaga ttagcagtag gctcatcagc aagcacaatt gcaggcttca
     1441 taacgagagc gcgagcaatc gcgacacgtt gacgctggcc tcccgagagc tctgctggct
     1501 tatgcagcat acgatccttg aggccaacgc tatcgataag aaaatccacc cacttttccc
     1561 tttctgccct ggtctcattt tctgaaagac catcgagaag aagaggaagt tccacattct
     1621 cgcgaatatt gaggacagga ataagattga aggtctgaaa gatgaatcca atagagcggt
     1681 gacgcaactc tgtgagtcta tcgtcgtcga gatcgctcgt acgctgtcca tttactatca
     1741 cagtgcctga agttggcttg tctacacagc ctatcaaatt gagaatggtg gtcttaccgg
     1801 aacctgaagg tcccgcaatg cttacaaaat cgccagagtg tatttcaagc gaaatgtctc
     1861 tgatagcttc gacctcgaca gtatctaaga aatagacctt ttgaacggca tcgagctcaa
     1921 taattatgtc tgacatgcga cttccttctt caaattttcg agtaaatcct aatgtttagc
     1981 caaaattgtt tccataagaa tgggttgaaa aagaaatgaa taattataac aacaattaca
     2041 atctgactgg gtataccttg aatcaggtta tttctatcct ctacttattt ggcattgcgc
     2101 tccatattta cctgggctaa aatgacaagc atggtaaagc gctgcctagt ttgcatcact
     2161 ggcgcttctg gcgctatcta ctccatacga ttactctgcg cacttgcggc aaatggtgtc
     2221 aggatccatg ttgtgacaag cgagcgcggc gcacgagtcc ttctcgaaga gacaggtcgt
     2281 ccacttggct attggctcgg aaggctgcaa tctcaaggag gtccggaagg aaggcatgct
     2341 tttattacca tgcatgagaa caatgatttc tcggcaccta tttcatcagg ttcctttcgg
     2401 ctccatggaa cggttatcgt accttgttcc atggggacgc taagcaccat agcaagtgga
     2461 aactgttcga atcttatcca tagggctgga gctgtcgctc taaaagaagg ttggcctttt
     2521 atcgttgtgc ctagagaaac accgctttcc cttatatcgc tgcgagccat gacccagctc
     2581 aaggaggctg gggctgtgat tcttccagct tctcctagct tctgtggcaa gccacccgac
     2641 atccagtcgc ttgtcgatac tgttgtatac cgaatacttg atcatcttga tatagttgac
     2701 aaagctatga caggagagga tccaggcaaa tcataaggca aaccataatt tctttgcgcc
     2761 aatcttagaa aggcaaaagc ccagaaccct taatgtctgc ctttcaagtc ctatattatt
     2821 tcgattgctc ctctgcttgt tcctcggtgg gcagcgctct gacctttctt tcgttgaaac
     2881 ctatccaaac ggtttctccc tcggaaggaa tgaccttacc caatggatct tcgatttcta
     2941 taagcagttc gccggtctct gtctttacat agatttccac atttgagcct aaatagacct
     3001 tggcaaagac ttgcccagaa gcaacagcct cttcttggct gacaatgcgc agcgattccg
     3061 gtctggccat tagttcaaca tcatcgcctg gcttaagccc ttttacagca gaactggcat
     3121 gcaccatttt ttgccctaat ctgcatatag cccttccatc tggttcaaaa ccctctagct
     3181 gcgcatggaa aaaagccacc ttacccacaa agcctgcgac gaatactgaa tcgggttcca
     3241 catatatttc gcttggagtt cctatctgca aaatcttacc ttcacgcatt acaattattc
     3301 gatcagaaag actcatagcc tcgatgcggt cgtgtgtcac atatatagcc gtaatgccaa
     3361 gggttttctg gatacgtcgg atttcgactc tcatctgctc gcgtaaaaga gcatctagat
     3421 tcgacagagg ctcatcgagc aatagtactt gtggctccac tacaagcgat ctagccagcg
     3481 caactcgctg ctgttgacca cccgaaagcc taccgggtgc tctgtgcatt agttcagaaa
     3541 gcccaacaag gtcaagaata tccgtgacct gcttttctat ttcattcttg ggtagctttc
     3601 gtattttgag cccataagcg acattgtcga acacatccat atgagggaaa agaccatagg
     3661 attgaaaaac ggtggctgtc tgcctcccat ttggcggcaa aaaagtcacg tctgtatctc
     3721 cgatgatgat cttgcccttg gttggcaatt caaagcctcc gatcatccgc aaagtagtag
     3781 tctttccgca tccagaagga cccaataggg tcaccagctc acctggcttt acttcgaagt
     3841 cagcatcctc gacagcatgt acttcgcctt tacctttcgg gtctttgaat atcttagtca
     3901 catgagagag cgccacaggc actgattttg ctgtcatact ctaccctttc ctataaaatt
     3961 aaatctgctt ttcggtcgac ccgcttcgat cgcgattcat cattgtgttg ttatcgtttt
     4021 ttccaggtac tcgctttgcc gcaccaaaag tctcattagc ccaaaaacgg ctagcactat
     4081 tatgattagc gtcgtagcga gcacactagc cagtccaaaa cgcaaattct ccgaaaagtt
     4141 atatatttgt atggtgatat ggtaccatct cgcgcttatg aggaatatta tagcggaaac
     4201 ggctgtcatc gagcgaacaa aagtataaga catgctcgat ataaaagccg gtcttatcag
     4261 tggcaaaata atggtgcgaa atatcgttgc ctgatctgct ccgaggtctg ccgctgcttc
     4321 ttctatcgaa ggatcgatct gccttagcgc cgcgataccg ccctccacac cgaccggcag
     4381 ctctctcacc acatagttta tgactaaaat tgcccctgtt ccgacaagaa gcaagggggg
     4441 tttgttaaat gccagaataa agcttattcc tatgagagta ccaggaatgg caaaaggcgc
     4501 catgatgaga gattctagga ttcgtttccc tggaaaattc tttcgcacga gtatgaaagc
     4561 cgccaccatc gcaagaattc ccgcgatagg agtggcaatt ccagccagtg tgattgttga
     4621 aacaagagat tgctttcctc ttgtcaaagc ctcgccgata ttagcaagag taaaggaata
     4681 atcgataccc caattcttga caaagcaacc agccactatc gtaccgtaca aaccgacaat
     4741 aaaggcgctt accaaaatta caaagcctgt caacgccttc cttactccag aagatgcgag
     4801 gtcagagagc ctcgtagaag gcttgccggt aacaaccaca aaagacttgc gattcaccca
     4861 gcggcgctgc gccagaaatg ccgtcagagt gggtaagagc agaagaagag acagagccgc
     4921 gccattattc aacctattca tacctatagc ttcaatatat gactctacag acagcaccct
     4981 ataggagcca gatagcagaa ggggatttgc aaaatccgca agggaatttg tgaacacaag
     5041 cagccagctg gaaagaaggc cgggggcggc aagcggcaat gtcacatggc tgaaggtata
     5101 ccatctggat gcattgagat cgagcgaagc gtcctccaat gtggagtcaa tagcctccag
     5161 gactccttgc atagtcataa atgcgatagg aaacatgctg attgtctgcg ctaaagccaa
     5221 tccccacaaa ccatatatat tgacattttg aaggccaagc cattgcctgg taacgatacc
     5281 attcttgcca aataacagga tgatagacaa agttagagag aatggtggcg atatgacagg
     5341 cattgtcgca agagtggtta tcaatttttt gccttttatg cttgtgcgcg ctaccgcaaa
     5401 agcatagaca aagcctatga aagttgaaat cgtcgcggtc acaattccaa gctggatcga
     5461 accccaaagc gcctgcaaat actggcgatt ggaaagaata ttcttccata cattgaaaga
     5521 gaatctgcca tcctttatca aggtaagacg cacggcctcc aaaagcggat aagcgacgaa
     5581 cgcaagcact attgcggcta ataccactat tgcataaccg ctgatcggat cacgcgagaa
     5641 tgtcgcaatg ccaaagccaa tggcaaacaa agcaaagaaa atcaaggcaa gagcatctac
     5701 aagcctggta aaagaaacat aaccagtaga gtcaatggca gcaagaagag tagcccgcgc
     5761 ttcgctattc tctgcatcgg gtatgggagc gaccataagc gtgtaaggga tccccttaaa
     5821 atggacgatt ttcggccatt tgtagatttc aaggtaagag accgattccg cagcgctgct
     5881 cgccgtatct gcatattccg gaagctgcca taactccaag agcgcgtcat ctccgccgag
     5941 ttcttcactt ccctcctcgc caggaactcc ctttatcaca aagaagcggt tgtccgaata
     6001 gatggcacca agctcttcta tccaatcaga aagagcttca ttgttcgaag gcgcaaaaga
     6061 agtcatggcc tttacgagcg ccttcatctc ttttagggtg ttcttttcga aggtattctt
     6121 aattgtagag aaaatccaat attctcctaa agcgaagata aggcatacca cagagagcat
     6181 tataatcttt ttcttggtaa acacttggag atcctcttct ctttatcatc atcaaaacca
     6241 agaatacgaa gaaccgaacc cttgaggaac ataacattcc tcagggtccg gctcattaat
     6301 ttttttcaca tttcggaatg tgatcctaaa taagatcaaa aatcagcgct tggaaccgat
     6361 ctccttgacc catcgctcga gaagccgatc cttattcgct gcatcccaca tcacatcctg
     6421 atcaacggtt ttgatctcgc ttagactgaa ggcttgagga ttcttcttag caatctttga
     6481 tacaggaatt acataccatg atgcaataat ctcctgcgcc ttagcagaaa gcacccagtc
     6541 atatagtttt ttggcattta ccgggtcggg tccgcctttt actatcgaca tagaggctat
     6601 ctcataaccg gttccttcgg aggggaaggt gatagtgaca tctgcacctt caaccttgag
     6661 cttgacattg tcgtgcgcat agccaatagc tacaggaatt tctccgatag ccacgctctt
     6721 tccaggcgcg gcgcccgatt tggtatactg gtcgatattc ttgtcaagct tattcagata
     6781 gtcaaagacc ttctcttcat cccccccaaa tacataacgc aatgtggtta tgacattgta
     6841 ggccgtacca gatgttgtcg gatttgccac gcgtatttta cccttgtatt caggttttag
     6901 cagatcagcc caggacgtgg ggggtttaat tccttgttct ttagctacct tattgttggt
     6961 tacaaagcag atgggcccta tataaagccc tacccaatag ttctctggat ctcgaaactg
     7021 caaaggagta ttctcgagca tcttggattt gtaaggagct gtcaagcctt tgagcttggc
     7081 gcttatgtga ttgagtccta ctccgcccac ccatatggaa gcctggggat ttgccttctc
     7141 tgcctcaagt ctggcctcta cttcaccgcc ggaaagtctc tgccaattga gcattattcc
     7201 cgtttctttc tgatactcat caaatagcgc tttagcgaga ggctcatcca gtgttgtata
     7261 tacgccgaat ctctgctgtc caaatgccac agtcagactg agaattagca caagccctgc
     7321 gatcataaga cgtttcatgg aaacctccta aaagtacttt acaaatatag tgtattccat
     7381 gacttctatt ttcgcaaggc agaatattgt ggcaaatagg attcagcaaa tagctaagaa
     7441 gatcaatgat tcaatctgta tcattattat acaagtctac tggcgcatat caatacaaat
     7501 gtatcaatcg gaaatataaa tccaagaact gttcttctga ttatgtctta ttgaaaccga
     7561 tatgagagct ctttatctac atctatagca ggcttgctgt ccggatatcg caggcagttt
     7621 gttatattat aatgaattat atttaattgt caatttgttt ggatttggca cattttttgc
     7681 ttatattata tatgagcgcc aaaaggggct ccggacaggc tatgagatcc tgtgtggatc
     7741 gacacctgct gatcctaaat agtaggaggt tgactatgaa caatctggta ttgtatgaca
     7801 aaagcccatt tgatcttatc gagagccttt tcaacaacga cgattggcta atgccgagct
     7861 tccgcactcc agccatagac gtatatgaag aaggcgataa atacaccatc gaggccgatc
     7921 ttcccggcct aaccgaaaaa gatatcaaac tggaagtccg gaatggccag cttattcttt
     7981 ccaccgcaaa acgagagaag agcgaagata aatccaagaa aggtcggtgg atccgcaggg
     8041 agcgcagaga gtaccaattc cagcgcagtt ttactcttcc tgaagaggta gatcccgaaa
     8101 agatcgaagc ccacttcaag aatggtgtgc tgcaggtcgt gcttccaaag aagcctgaag
     8161 ctgctcccaa gagcatcgag gtaaagatca actaagctgc gcttcttagc agagcttctt
     8221 tgttgctttt cttaatcttc cttaatagaa accgggcttt aggcccggtt tttttatagc
     8281 acggctttca gctctcagcc ttcttgtgtt atcatgctga acgaattcta gatttacata
     8341 accggctttg ccggcaaagg agatattatg gcggataaat ctaatcgcct tcctcaaaac
     8401 gcaaaaggca aatggtatgt cgacgcatcc tgcattggct gtggcctatg ctctgttacg
     8461 gcaccagaca tttttattct aggcgacgac gggcaggctt atgttgctcg tcagcctcag
     8521 acggaagagg agaacgagct ggcaattcag gccatgaacg attgctctgc acaatcgatc
     8581 ggagatgatg gagagtaagc tctctagtct cctctaaccc ttgagagctt cggggtttaa
     8641 tcctcgcaac tcaggtaagg taaagagtcc atcctttcgg atgagtttac catcgatcca
     8701 tacttctccg ccgcccgcag cttcatcctg caaaagtact atatcccagt gcagggcgga
     8761 gcgattgcca ttaaaacaat cgtcgtacga gtttccgggt gttatatgca gagatcctgc
     8821 gattttttca tcgaaaagcg tatccaggat tgggcgggta atataagggt tgataccaaa
     8881 agaaaactcg ccaagataac gagctccttc gtctatatca agaatcttat tgatccgttc
     8941 ggtatcgtta gcacgagccc ggacaatttt tccatctttg aattcgaaaa cgatattctc
     9001 gaacttaaag ccatctttct cgtttggagt attatagctg agtatcccat ttaccgaatc
     9061 acgaaccggc gcggtataga cttctccgtc tggaatgttc atagttccat cgcatttgat
     9121 agcgggaaga ccttttaccg aaaattcgag atcggtgtca tggcctaata tcttgactct
     9181 atcgctcttc tgaagaagct ccacaagcgg atccatagcg cgagacattc tgctatagtc
     9241 cattgtacat acatcgaaat agaatttttc gaatgcatct tcgctcatcc ctgcattctg
     9301 tgccattgct gcgctaggat agcgcagcac aacccagcgg gtatgtggta ttcgccgatc
     9361 gatgtgcact tttttccaga catggttatt gtagagctct atcttgctgg ccggtaagtc
     9421 ttgccatgca aatgaatttc gaacgcttgt aaaaccgata tatacatcca tcttgtccat
     9481 gcgagcgagc tcgaattcgg cttgtagttc gaactgctcg tcaggcgcat ccataaagag
     9541 tgttctttcg atccgtctat ctctcaagga aatgaaagcg agtgctcccg ccctatgcac
     9601 ctcttctatg agaaggcgtg taaagtccgg ttcaaggttg gagttttcaa tgagcacctt
     9661 atcgccattt tgcactctgg tcgaataatt taccaagagc tgcgcgagtc ttctgagtct
     9721 ttgatcttcc atcgattact tcctttgcga ataagtttga ttttgcaaaa aatttatata
     9781 cactctcttg aatatcgtct tattgaatag cgccatatat tggaaccaat attcaagaaa
     9841 tccaaaaagc gatccatcca tctcaatagt acacaaatat agaataaaaa accattgacg
     9901 ccatcaagaa actgtagtta aaatgaaata tccgttttct gataggagga ctgcatga
//
LOCUS       DAOMZL010000063         9821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_6527_length_9821_cov_16.661479, whole genome shotgun sequence.
ACCESSION   DAOMZL010000063 DAOMZL010000000
VERSION     DAOMZL010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 9821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9821
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6527_length_9821_cov_16.661479"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1756)
                     /gene="glgP"
                     /locus_tag="PLQ30_05975"
     CDS             complement(<1..1756)
                     /gene="glgP"
                     /locus_tag="PLQ30_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPW02029.1"
                     /translation="MKILSHTVKPALKGRLTKLDLIARNLWLSWNFDAVSLFTRIDRG
                     LWSASGQNPVRMLGMLSQEKIDELASDPAFLSDLDEVYSNFQRYLKNRIWYRGPRDRV
                     IAYFSMEYGLDVSLATYSGGLGVLSGDHLKTVSDLGLPLVAVGLLYRQGYFKQYLNAD
                     GYQLETYPENDWYNMPVERCVDAQDAPVLIQVEMAGRPISAGIWRVDIGRAQLYLLDT
                     NIPENKPEDRIITATLYGGDMEMRIKQEILLGIGGIRALKALGIHVGATHMNEGHSAF
                     LALERIRVLMEEYGLDAHSAIQAFIPTNVFTTHTPVPAGNERFGIELMEKYFRSFVTA
                     LGLDWNEFLGMGRENPLDKQESFCMTVFALRLSVRSNGVSKLHGEVSRRMWKGLWPEL
                     DLKEIPIGHVTNGVFPRTWISHDMLSLLDKFLGPKFELEPDNPDVWAAIDQVSDEELW
                     RTHERRRERLVAISRQRLAATYKRLSLPDGEIVRSSDVLSPYALTLSFARRFATYKRG
                     TLLLKDPDRLIRLLNDKDKPIQIVIAGKAHPHDMAGKELIREIIHFSRREEVFGRLVF
                     IEDYDMAMGRYMTSGSDVW"
     gene            complement(1939..3669)
                     /locus_tag="PLQ30_05980"
     CDS             complement(1939..3669)
                     /locus_tag="PLQ30_05980"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW02030.1"
                     /translation="MERFLERSGYAEDRILQFFARLIGDQFFVQKKDILVHDEDLGYR
                     SPLITALIESYIEDSKTSATSKISGSEEETREKQARIRALVLTEHEEVLIDLQKLFAI
                     ASFDSLVISSDTALKEANMPLSERTVVAMPTSLFLAATQVPEFKAREFGLVVLEGADL
                     FGDTPGEVQRRIWGYLLPPWQRRAAIFAKHIGIRTRNTALDFANDPKTIVLKKAQASL
                     SQISTYMYRLSSQHKFRVLLAMIQENFQEDRRAIIVFCNLRQTSMELEFRLKLNNIKA
                     KQVSISAPKAERKELLLEFAEIEKMAEGGQFVLVVSNDNLSVLPSELANLAIHYDIPL
                     DPDIYLERVRIMRPSSSTIIGLVCERYEVGLSAINSKFGMQFELREPSYDMLEYRDES
                     EGVPLKIEYERRSSEYKPRRRIEQSKDKTEDAKRIKKTQHRELKENFHQGQKAQHNRK
                     NSSPKKSPGVDQNSELYSMSTDERLAYFRQKYRGILNSPIEPKPHPKKGEKTISRSAS
                     EPLSFENTIPTKNADGERSISAADSIEVRKGDKKQASGMESDNPSIVKRFIEKLFPGT
                     NEGTDEGIKE"
     gene            complement(3861..4646)
                     /locus_tag="PLQ30_05985"
     CDS             complement(3861..4646)
                     /locus_tag="PLQ30_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPW02031.1"
                     /translation="MRYRKGASLVSDTAISRLIGDVLLAFIGLIAWPLCHLLYNIKIE
                     KIGRAKTERLPSRAVLVSNHCMPLDPVFHGLSILPRRTFFTLLEETCEAPVIGPLVRL
                     LGGIPLPRNRNRLGDIEMAVEHALNTRGLVHFYPEGECFLGNQDIFPFKAGAAYFAIK
                     FGVPVIPIVTILKRANGRKSKRIQVNVHMLEPIMPLALLDKPLSLSSMAKNPAQENSR
                     EMLSRAIECTRKFHALMQAEIDFSGCDKSLYKGPMPRIKGVND"
     gene            complement(4643..5782)
                     /locus_tag="PLQ30_05990"
     CDS             complement(4643..5782)
                     /locus_tag="PLQ30_05990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW02032.1"
                     /translation="MISTLLNAYGLIVFAACVFFFFITVSNILWLMHTLTIERKKNGP
                     SVAVLIPARNEALRIRPCIDSLLSQDYKSYQIYVIDDNSDDETWNILKSYMIRFPSKF
                     KAFKAEPLPENWYGKPHALQELATHVKENYLLCTDADTIHKPDSISKAMAAAEKYKAD
                     LITGYAHHLMPTFAEAAIVPSMYILTMLGMPLYLIPNTKSPLFSHAIGQFMLFKRSFF
                     EKIGGYEKVKHEATEDVRLSRIIKENGGKIAFIDLKDIVECRMYTTYKEAIQGIAKNA
                     YDYLGKNLLILFSGTVAVPLVFYVPLIVLFIDVPWLGQAIPYLKASAIITFYSWVLET
                     IDRRLPWYVPFIYPLIFVNTMSALWRGFHLVNKEGGIEWRGRKVK"
     gene            complement(5779..6624)
                     /locus_tag="PLQ30_05995"
     CDS             complement(5779..6624)
                     /locus_tag="PLQ30_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02033.1"
                     /translation="MNSLLHAQTNSVVLKESCQSSPPKNPAQIGKFQFDSQGFLSSNQ
                     DLLPGIPFPKGMMPPEVEAFLELRDNVYENAAPESVEAQAVSLLKRLSSLPVSISNQA
                     LLEARIAYLVGRAWNDHKDKNKAIPWFERAVEAAQRMINLEGETPTALVVLAEPLGEL
                     SILKDLGFLIKNGPKVGQYASKALELDPKNIKAWLLKASALAYPPPIWGGNYKKALET
                     YASVLLIAQNGLPKDVLFDLRVGIATAYSNLKLSEHATWWFDAALLLYPKNLYAKSEL
                     EKLSL"
     gene            6820..8124
                     /locus_tag="PLQ30_06000"
     CDS             6820..8124
                     /locus_tag="PLQ30_06000"
                     /inference="COORDINATES: protein motif:HMM:NF015382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HPW02034.1"
                     /translation="MCDTLILHNSRGNPFIFGKNSDRHPEEPQALVYIPAQEPKVAKL
                     GGGIEYPDMSYDFLLSKPSWIDGGEMGLNNRGLAIGNEAVFSRFKPDKKGVLGMDILR
                     AALSVCATAKEALNFISSFVERFPQGGNGAYKGKLFYNNSFLIADPDEAYILETAGPR
                     WAWRKAMIADAISNIYCIEDDFTCIDAMTRKEIALVNDKGDCSDETDRDRKEQRGSWK
                     KYVEDKKYLFFTQGEQRRSTSLAGLLRIAAESDKTEGGQILFSSAIEPFFAILRSHKG
                     ASKPGAFLNRMKNVCIHPGIFPKSATTASMVIEYCKGGAIIWYTDSSFPCVSLYKPVL
                     LKDGHFYALWKPLLTENNAEKSYSYWEARKNWASKPRKLELSSQQAFVQSRDIAQKSI
                     VEIAHQAFPAMIKEKTSTERMLSVYASEVAAIVGEWEEHWIN"
     gene            8363..>9821
                     /gene="ggt"
                     /locus_tag="PLQ30_06005"
     CDS             8363..>9821
                     /gene="ggt"
                     /locus_tag="PLQ30_06005"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPW02035.1"
                     /translation="MKKSILALMLLIAAIATISAQLPVNLYGRAAVGENGVVAAAKPE
                     ASQVGIDILKKGGNAVDAAIATGFALGVLEANANGIGGGGFMIIKMVDMAEPVVIDFR
                     EKAPGKATPTMYLGPDGKVIPNSTIEGGLSVGVPGEVKGLLYALENYGSGKLSRADII
                     QPAIQWALQGIPVTVNLESIIKDNYGKLVKYENGAQIYLKDGLPYEVGDVIYNPDLAR
                     TLAKIVKEGADAIYKGPIAQAMVDEVQKRGGILTLEDLANYEIKVRKAVEGEYRGYKV
                     YSVPPASSGGTHLIEILNILENFDVVKLGFQTAQASHLWSEILKLTFADRAKYMADTD
                     FVQVPLKGLTNKEYAKQQATRINMNASMSSPQPGDPFKYESGSTTHFSVMDKAGNMVA
                     VTKTINYFFGSGVVIPGWGFIMNDEMDDFVPTPGSVNSVEPGKRPLSSMSPTLVLDPE
                     GRSFMAIGSPGATRIFPTVAQVISNVIDFGFPIQEA"
BASE COUNT         2579 a         2262 c         2248 g         2732 t
ORIGIN      
        1 gccaaacatc tgagccactc gtcatgtagc gacccatcgc catatcatag tcttctataa
       61 acacaaggcg accaaagact tcttcacgcc gagagaaatg tatgatttct cgaataagct
      121 ctttgccagc catatcatga gggtgcgctt ttccggctat tactatttga ataggcttat
      181 ccttgtcgtt aagaagcctt attaatctat cagggtcttt gagaagcaag gttcctcgct
      241 tatatgttgc aaagcgacga gcaaaactca atgtgagcgc ataaggagaa agcacatcgc
      301 tggagcggac gatctcgcca tcgggtagac tgaggcgctt atatgtcgcg gcaaggcgct
      361 ggcgacttat tgcgacaagg cgctctcgcc gacgttcatg tgttctccat agctcttcgt
      421 cggatacctg atctattgca gcccatacat caggattgtc gggctccagc tcgaattttg
      481 gcccaaggaa tttatcaaga agactgagca tgtcatggct gatccaggtt cgggggaaaa
      541 ccccatttgt tacgtgtcca ataggtatct ctttcagatc cagctctggc cacaagcctt
      601 tccacattct gcgcgagact tcgccatgca atttggacac gccattcgat cgaacggaca
      661 aacgcagggc aaagacagtc atgcagaaac tctcctgttt gtccagggga ttctcgcggc
      721 ccatacctaa gaattcgttc cagtctaagc ctagcgcagt tacaaaagag cggaaatact
      781 tctccattaa ctctattccg aagcgctcgt tacctgcagg tactggcgta tgtgtcgtga
      841 agacattcgt aggtataaat gcttgaatag ccgaatgtgc atccaggcca tattcttcca
      901 tgagtactct aatcctctcc agggctagga atgcggaatg tccttcgttc atatgggttg
      961 cacctacatg gataccgagt gccttgagtg ccctaattcc tcctatgcct aacaatatct
     1021 cctgctttat ccgcatttcc atgtcgccac cataaagtgt cgctgtgatg attcgatctt
     1081 ctggcttatt ctcgggaata tttgtgtcaa ggaggtaaag ctgagctctt ccaatatcta
     1141 cacgccagat tccggcgctg attgggcggc ctgccatttc gacctggatg agcacaggtg
     1201 catcctgggc atccacacag cgctccaccg gcatattgta ccaatcgttc tcagggtatg
     1261 tttctagttg atagccatcg gcattcagat attgcttgaa atagccttga cgataaagaa
     1321 gcccaacagc cactagcggt aatccgagat cgctcacggt ttttagatga tcacccgaga
     1381 gcacacctag tccgccagaa taggtcgcca aggagacatc gaggccatat tccatagaaa
     1441 aataggcaat tactctatcg cgtggtccgc gataccagat tctgttcttt aggtatcgct
     1501 ggaaattaga ataaacctca tccaggtccg agagaaaagc agggtcggag gcaagctcgt
     1561 cgatcttttc ctgactcagc atgcccagca ttcttaccgg attttgcccc gaggccgacc
     1621 acagccctcg atcgatgcga gtaaagagtg acactgcatc aaaattccac gaaagccata
     1681 ggtttcgcgc aattaaatcg agtttggtca gacggccttt aagcgctggt ttgaccgtat
     1741 gcgacaatat tttcatttat aaatacctct tttcgttttt agaataggat tcctgccttc
     1801 actttcccgg tctggtctat ctgctctcat gtttctgctt tcctattgtt tgttgctatc
     1861 ggactcagga aaggccatgt ccgggaagga tgactcaagc atttgttcgc ttgtctgctc
     1921 gctaatttgc gccttcatct attcctttat tccttcatct gttccttcgt tcgttcctgg
     1981 gaacagtttt tctatgaacc gttttactat acttggatta tccgattcca tgccactagc
     2041 ctgtttctta tctccctttc taacctcaat tgagtccgcg gcgctgatcg atcgctctcc
     2101 atctgcattc ttagtaggaa ttgtattttc gaaagacaat ggctctgatg ctgacctgga
     2161 tatcgttttt tctccttttt tgggatgcgg ttttggttca ataggcgaat tcagaatgcc
     2221 acggtatttc tgtcggaaat aagccaagcg ctcatcggtg ctcatggaat agagttcgct
     2281 attctggtct actcctggac ttttcttagg cgagctgttt tttctattat gttgggcttt
     2341 ttgaccttga tggaaatttt cctttaattc tctatgttga gtctttttaa tcctctttgc
     2401 atcttccgtt ttatctttgc tttgctcaat cctgcgtctc ggtttgtatt ccgaagatcg
     2461 tctttcatat tctattttca atggtacccc ttcgctctca tcgcggtatt ccaacatgtc
     2521 atagctgggc tctcttagtt cgaattgcat gccaaatttg gaattaattg ctgataagcc
     2581 gacttcatag cgttcgcata ctaggcctat tatggtcgag ctacttggtc gcattattcg
     2641 cactcgctca agatatatat cagggtctaa ggggatatca taatgaatag ctagatttgc
     2701 caattcggaa ggcagcacac tcagattatc attggagaca acaagaacga attggcctcc
     2761 ttctgccatc ttctcaatct cggcaaattc caggagaagc tcctttcttt ctgctttcgg
     2821 agccgaaatg gatacttgct tagctttgat attattgagc tttagcctga attccagctc
     2881 catcgatgtc tgtctcaaat tgcagaatac aataatagct cttctatctt cttgaaaatt
     2941 ctcttgaatc attgcaagta gcaccctgaa tttatgttgc gaacttagac gatacatata
     3001 ggtgcttatt tggcttaagc ttgcttgggc tttttttaat acgatcgtct tgggatcatt
     3061 tgcaaaatcc agcgctgtat tccttgtcct tatcccgata tgcttggcaa atatggctgc
     3121 cctccgctgc catggtggaa ggagatatcc ccatattcta cgctgcactt cgccaggcgt
     3181 gtctccgaat aggtcggctc cctcgagcac gacaaggcca aattcgcgcg ccttgaattc
     3241 aggcacttgt gtcgcagcca gaaacagcga tgtaggcatt gcaactactg tcctctcgga
     3301 gagaggcata tttgcttctt tcagggctgt atccgacgat atcaccagac tatcaaaact
     3361 agcaattgca aatagcttct gcagatcgat caatacttct tcatgctcgg tgagcactaa
     3421 ggctcgtatt ctggcttgct tctctcgcgt ctcttcttcg cttccagata ttttggaggt
     3481 cgcagaggtc ttggaatcct cgatgtagct ctcgataaga gcagtgatga gtggcgatcg
     3541 ataacctaag tcttcatcgt gtacaaggat gtcttttttc tgaacgaaaa actgatcgcc
     3601 gatgaggcgt gcgaagaatt gaagaattcg atcctctgcg tatccactac gctccaagaa
     3661 tctttccatt tccaaaccat ccctatattg caatatctat atataatgca ccggtgcatc
     3721 tttgtaacat aatcataaga aagaaatacc tatcatatta gataatcggc attaggtata
     3781 ttgtagagaa tacataagat ctgattatgc aatggctgca tgcccatact gctacgaacc
     3841 ttgtaaggtt cgtagcagta ctaatcattc acccctttta ttcgcggcat aggtcccttg
     3901 tagagtgact tgtcgcaacc agaaaaatct atttctgcct gcataagagc atggaacttt
     3961 cgtgtgcact cgattgctct agagagcatc tcgcgtgaat tttcttgggc tggatttttg
     4021 gccatgctcg atagggatag gggcttgtct agcaaagcca ggggcatgat aggctcgagc
     4081 atgtgcacat tgacctggat gcgctttgat ttcctcccat ttgcgcgctt taagatcgta
     4141 acaataggta tcactggcac gccaaacttt atagcaaaat atgcagcgcc cgccttaaat
     4201 ggaaatatat cctgatttcc cagaaaacat tcgccttctg gatagaaatg gactagccct
     4261 cttgtattaa gagcgtgctc tacggccatc tcgatatctc caagcctatt tctgtttctt
     4321 ggcaagggaa tcccaccgag cagtctcact aaagggccga tcaccggtgc ctcgcatgtt
     4381 tcctcaagga gagtgaaaaa agtgcgacga ggcaaaatgg aaagaccgtg aaagacggga
     4441 tccagaggca tgcagtgatt gctcaccaga acagctcgag atggaagcct ttctgtcttc
     4501 gccctgccga ttttttcgat cttgatatta tatagaagat gacagagtgg ccatgctatc
     4561 aagccaataa atgccaggag aacatcgcca atgagacgcg atatggctgt gtctgataca
     4621 agagaagccc cttttcggta tctcatttta cttttctacc cctccattcg attccacctt
     4681 ccttgtttac aaggtggaat ccgcgccaaa gcgctgacat tgtattgaca aaaatgagtg
     4741 gatagataaa tggcacatac catggtagcc gacgatcaat tgtttcaaga acccaggagt
     4801 aaaatgtgat aattgccgaa gctttgagat aaggaatggc ctgcccaagc catggaacat
     4861 cgataaaaag cacaatcaat gggacataaa agacaagggg cacagccact gttccggaga
     4921 aaagaatcaa gaggtttttg cccagataat cataggcatt cttggcaata ccctggatgg
     4981 cttccttata ggtcgtatac attcggcatt ccacgatgtc ttttagatcg ataaaggcaa
     5041 tctttccgcc attttctttt atgatcctcg atagtctcac atcttctgtc gcttcatgtt
     5101 ttaccttttc atagccacca atcttttcaa aaaaagacct cttgaaaagc atgaactgcc
     5161 cgatagcatg gctgaaaagt ggagattttg tatttggaat caggtagagt ggcattccca
     5221 gcatagtaag gatatacatt gagggaacaa tggccgcttc tgcgaatgtg ggcatcaagt
     5281 ggtgagcata gccagtgatc agatcggctt tgtatttttc cgcagctgcc attgctttgc
     5341 ttatggaatc tggtttatgg atcgtgtctg catctgtgca tagcaagtag ttttctttga
     5401 catgagtagc taattcctgt agtgcatggg gtttgccata ccaattttct ggaagaggtt
     5461 cggccttgaa tgccttgaat ttggatggaa atcgtatcat atagcttttc aggatattcc
     5521 aagtctcgtc gtcggagttg tcatcgataa catagatctg gtaggactta taatcctgag
     5581 ataacagaga atcgatgcaa gggcgaatac gcaaggcttc gttgcgcgcc gggatgagta
     5641 cggctacaga tggcccgttt ttttttcgct ctatagtgag tgtgtgcata agccataaga
     5701 tgttggaaac cgtgatgaaa aagaaaaaca cacatgcggc aaagacgatt aacccataag
     5761 cattgagcaa agtgctaatc ataatgacag tttctccaat tcactttttg catagaggtt
     5821 tttgggataa agtagaagcg cggcatcaaa ccaccatgtt gcatgttcgg agagtttgag
     5881 attgctgtat gccgtggcga taccgacacg taaatcgaat agaacatctt tgggcaagcc
     5941 gttctgggct atcaatagaa cgctggcata ggtttccaag gcttttttat aatttccgcc
     6001 ccaaataggc ggaggataag ccaaagcact cgctttgaga agccaggctt tgatgttttt
     6061 tggatccaat tcgagagcct tactggcata ttggcctact ttgggaccat ttttgattag
     6121 gaatccaaga tctttcagaa tactaagctc gcccaatggc tcagcaagca ctacgagggc
     6181 tgtaggtgtc tccccttcca ggttgatcat tctctgagct gcctccactg cgcgctcgaa
     6241 ccagggaatg gccttgtttt tgtctttatg atcattccaa gcccgaccaa ccaaatatgc
     6301 tatacgcgct tcgagcagtg cctggttgga gatggataca ggcaggctgc ttagcctctt
     6361 tagcagtgaa actgcttgtg cctctacaga ttccggagca gcgttttcat acacgttgtc
     6421 gcgaagctca agaaaggctt caacttcggg tggcatcatg ccctttggaa aagggattcc
     6481 aggcaaaaga tcctggttgg aagatagaaa cccctggcta tcgaattgga acttgccgat
     6541 ttgagctggg ttttttggtg gactggattg gcaggattcc ttaagaacga ccgaattggt
     6601 ctgagcatgg agcagactat tcacggatat taatttcgag atattcatca tcgctagaac
     6661 aaaaatcgcg attcgtaata tcagatcaaa gcgtgcctta tttgaatcca ttataagcca
     6721 ggatagcaga cttttgcgct tgcgggaata ttgaaagttg atatacgata atacatcgac
     6781 ttggggtggg gattaatccc cgaggaggta tacaagccta tgtgtgatac tctgattctg
     6841 cacaatagcc ggggaaatcc ctttatattc ggcaagaatt ccgatcgaca cccagaagaa
     6901 cctcaagctc ttgtctatat tcctgctcaa gaacccaagg ttgctaaatt agggggagga
     6961 attgagtatc cggatatgag ctatgatttt cttctctcca aaccttcttg gatcgatgga
     7021 ggtgaaatgg gcctcaataa tagaggtctt gctattggca acgaagcagt cttttcgcgc
     7081 ttcaagccag ataagaaagg cgtgcttggc atggatatcc tcagagctgc gctatcggtc
     7141 tgtgctactg ctaaagaggc tctcaatttc ataagttctt ttgtcgaaag attcccgcaa
     7201 ggaggcaatg gagcatataa gggcaagctt ttttataata acagttttct tatagcagac
     7261 cccgatgaag cctatatcct cgagacggcc ggaccgcgct gggcatggcg gaaggcgatg
     7321 attgctgacg caatatccaa tatctattgt atagaagacg actttacatg tatcgatgct
     7381 atgacgcgca aggagattgc tctggtaaat gataagggag attgttctga tgaaacagat
     7441 cgggatcgca aagaacagcg tggctcctgg aaaaaatatg tggaagataa aaaatacctt
     7501 ttctttactc agggagagca gcggagatcc acatcacttg ctggccttct gcgcatagcg
     7561 gcagaatcag acaaaactga aggaggccaa atactctttt cgagcgcgat agaacctttc
     7621 tttgcaattc tgcgctcgca caaaggagct tcgaaaccgg gcgcattcct gaaccgaatg
     7681 aaaaatgtct gtatccatcc tggtattttc ccaaagagcg ctactacagc ttctatggta
     7741 atagagtatt gcaaaggtgg cgcgattatt tggtacacag atagctcctt cccctgtgtt
     7801 tctctctata agccggttct cttgaaagac ggccactttt atgcgctctg gaaacctctc
     7861 ttaacagaga ataatgccga aaaaagctat tcctactggg aagcacgaaa aaactgggcc
     7921 tccaagccac gcaagctcga actttcaagc caacaagcct ttgtacagtc gagagatata
     7981 gcacagaaat cgatagtcga aatcgctcat caggctttcc ccgccatgat aaaggaaaaa
     8041 acatctacgg aacgcatgct ttctgtatat gctagcgagg ttgcggctat agtcggcgaa
     8101 tgggaagaac actggataaa ttaggaatgg atagagagtg cgtctctata cctgttcttt
     8161 tccttagaga aggctttctt atgccattaa caactcttat aaacaaggtt tgtagcagta
     8221 tatacggatt gagaattaga tcgagctcga tcgacttgtg agtattgagc ccaatttacc
     8281 gacaaagggt tgacacctgc caaaaactat aagaagctaa aaattgctaa atactataag
     8341 agaagaatga aaggaggcgc atatgaaaaa atctattctt gcgcttatgt tgttaatagc
     8401 agcaattgct actatttccg cccagttacc ggtcaactta tatggccgcg cggcagtagg
     8461 agaaaatgga gttgttgccg cagctaagcc tgaagcatcc caggtcggaa tcgatattct
     8521 taagaaaggc ggcaatgctg tcgatgccgc tattgcgacc ggcttcgcac tcggtgtgct
     8581 cgaagcaaat gccaatggca tcggcggtgg cggctttatg atcatcaaaa tggtagacat
     8641 ggctgagccg gtagtgattg atttccgcga gaaggctcct ggcaaggcta ctccaactat
     8701 gtatcttggc ccggatggca aggttattcc caattcgacc atagaaggcg ggctttctgt
     8761 aggagtaccg ggagaggtaa agggcttatt gtatgcacta gaaaactatg gctcaggcaa
     8821 attatcccga gccgatatca tacagccagc aatccagtgg gcattgcagg gcataccggt
     8881 cactgtcaat ctagaatcca tcataaaaga taactatggc aagctcgtta aatatgagaa
     8941 cggcgcccag atctacctaa aggacggcct tccttatgag gttggcgatg ttatatacaa
     9001 tcccgacctg gccagaacat tggcaaagat tgtaaaggaa ggcgctgatg ctatttataa
     9061 aggtccaatc gctcaggcaa tggtcgatga agtccagaaa cgcggaggca tcctcaccct
     9121 tgaagacctt gctaactacg aaataaaagt tcgtaaggca gtagaaggcg aatatagagg
     9181 atacaaggtc tactccgtgc caccggcctc ctctggcggc actcatctta tcgaaattct
     9241 caatatcctt gagaatttcg atgttgtaaa gctcggcttc cagacagcgc aggcatctca
     9301 tctctggtca gaaatactga agctcacctt tgcagaccga gcgaagtaca tggccgacac
     9361 cgattttgtc caggtcccgc tcaaaggact aactaataag gagtatgcca agcagcaggc
     9421 gacaaggatc aatatgaatg catcgatgag ctctccccag ccaggcgatc cattcaaata
     9481 tgaatcgggc tccacaactc acttctctgt gatggacaaa gcaggtaata tggtagcagt
     9541 taccaagact atcaactact tcttcggctc aggcgtcgtt attccaggct ggggattcat
     9601 aatgaatgac gagatggacg actttgtgcc gactcctggt tccgtaaatt ctgtagagcc
     9661 aggcaagcgg cctctttcca gtatgagccc gactctcgtc ctcgatccag aaggccgctc
     9721 ttttatggca attggatctc ctggcgcgac gagaatattc cccactgttg ctcaggtcat
     9781 ctcgaatgtc atcgattttg gattcccgat ccaggaagcg a
//
LOCUS       DAOMZL010000064         9558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_6778_length_9558_cov_12.251920, whole genome shotgun sequence.
ACCESSION   DAOMZL010000064 DAOMZL010000000
VERSION     DAOMZL010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 9558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9558
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6778_length_9558_cov_12.251920"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1248)
                     /locus_tag="PLQ30_06010"
     CDS             complement(142..1248)
                     /locus_tag="PLQ30_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HPW02036.1"
                     /translation="MANEIVPNPPEGQLLIYQDGALQVQVRIDGQTVWLTQRLMAELY
                     QVSVKTVNEHLVNIYAEQELDSSATIRKFRIVQTEGARQVSRMVDHYNLDAILAVGYR
                     VRSARGTAFRQWATARLSELLVKGFTLDDDRLKEGRTLGESYFDELLERIRDIRASER
                     LFYQKITDIYATSIDYDPQAEITQMFYATVQNKLHWAIHGHTAAEIVYHRADANKPRM
                     GLTTWKNAPYGPIRKTDVTVAKNYLTEEEIRELNRIVTMYLDYAEDQARRRKPMYMKD
                     WVKKLDAFLRFNERNIMTHAGMISRTLAEEHAHAQFDKYEAERRKLEATAPSSDFDRF
                     VEKTRQLTGQEKKKLPGPKKPRRTTSQKDKGDRK"
     gene            complement(1238..2830)
                     /locus_tag="PLQ30_06015"
     CDS             complement(1238..2830)
                     /locus_tag="PLQ30_06015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HPW02037.1"
                     /translation="MAKENNSGANLGFETELWRAADALRSNMDAAEYKHVVLGLIFLK
                     YISDAFEEHRIKLEADRSEGADPEDPDEYRAVNIFWVPKEARWSHLKANARQPTIGKL
                     VDDAMLAIERDNPSLKGVLPKDYAHPRLDKQRLGQLIDLIGDIGLGDKANRSKDILGR
                     VYEYFLSQFAGAEGKKGGQFYTPRCVVQLLVEMLRPYKGRVYDPCCGSGGMFVQSEKF
                     VEAHGGRIGDISIYGQESNHTTWRLAKMNLAIRGIDGNLGKEHADSFHRDLHPDLKAD
                     YVLANPPFNDSDWGGERLKDDKRWKFGIPPAGNANFAWVQHFIYHLAPTGLAGFVLAN
                     GSMSSNQAGEGEIRKSIIEADLVDCMVALPGQLFYSTQIPVCLWFIARDKKNSRFRDR
                     RGETLFIDARKLGTLIDRVHRELTYDDISKIADTYHAWRGDNVGADLRVSPDAQANTQ
                     VRAYEDIPGFCKSATTDDIRGHNYILTPGRYVGAAEVEDDGEPFEEKMAKLTAALREQ
                     TEQAKKLDQLIWANLEDIGYGK"
     gene            complement(2832..3071)
                     /locus_tag="PLQ30_06020"
     CDS             complement(2832..3071)
                     /locus_tag="PLQ30_06020"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW02038.1"
                     /translation="MLNAQADIPRLVRELRQRTGLTQEKFAAKLGVTYPTINRWENGR
                     AKPSPLALKQIEDLLRGLGDRGKDLLREFFTDEEI"
     gene            complement(3548..3620)
                     /locus_tag="PLQ30_06025"
     tRNA            complement(3548..3620)
                     /locus_tag="PLQ30_06025"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3585..3587),aa:Asn,seq:gtt)
     gene            3854..4417
                     /locus_tag="PLQ30_06030"
     CDS             3854..4417
                     /locus_tag="PLQ30_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02039.1"
                     /translation="MHRVKIAITAYEPVLYTEFYPIFEDSPFLIIIDEYNHVQKYSAE
                     IGSKEILKGRAEWIIGRGARILITGSIDNEDYWKLRLAGVTIRWESFGEVKSLVERAR
                     IAADHVQETMENRKPIDRSRYSKGLRANSFAAPCFVYFREEDIKYLERLEQKIEKEGK
                     KLLLQIKDEEGSNYDDYETSGNTTSKK"
     gene            complement(4436..6511)
                     /locus_tag="PLQ30_06035"
     CDS             complement(4436..6511)
                     /locus_tag="PLQ30_06035"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HPW02040.1"
                     /translation="MAYINKLLTENFLYYASYVIMDRAIPELDDGLKPVQRRILHSLF
                     EMDDGKFHKVANVVGHCMKYHPHGDASIASALINLANRGLFLDTQGNFGNPITGDEAS
                     APRYIECKCSPFAKEIFYHPKITRYIDSYDGRNKEPLVFPAKIPVILILGAEGIAVGM
                     STRILPHNPIEVIKAEIAYLQGKAFSLSPDFPAGGTADCSEYADGNGKVRVRATIDSS
                     DPKKIVIKEIPYGSTTESLINSIEEAVRAGHLKIASISDFTTSKVEIELKLARGVYAR
                     DVVDALYAFTECERSISCNCLVIKEGKPVVTTVSETIKYHAARLLDLLRQELELERDE
                     LKNELFSRTLERIFIEEKIYTSIETKRTNDTVIKAVLDGLKPFTKELSRKVEVEDVER
                     LLKIPIRRISLYDIEKAKREMEQIERRISENEMHLRHIVDYAIDILQSYIEMIEKICP
                     RKTKLRAFKRIEARAVARHDFALKYDAEGGYLGTEVSGEKLLEASKFDKILILRNNGI
                     YTIIPLPDKFFAGQKLLWTAIAKKESLSKTTITLIYSLSGENGAYIKRTIIDSWLTGR
                     DYSLLPETAVILGFSTASEFDFKLEYKPKSRSKKTYEVFHAKNYTVRNRGAQGIKLTD
                     REITRFEILTTQEPDNKRKQEDLDASTAHISKKKPNREARSKKEIPPLETISEGLLKA
                     ALKKQKKEN"
     gene            complement(6565..8370)
                     /locus_tag="PLQ30_06040"
     CDS             complement(6565..8370)
                     /locus_tag="PLQ30_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HPW02041.1"
                     /translation="MTGNKSTYDESKIKTLSSLEHIRLRTGMYIGRLGDGLSPDDGIY
                     ILLKEIIDNSVDEFIMGAGNQIVINLQGRRMRVRDYGRGIPLGKVIECVSVINTGAKY
                     NDDVFQFSVGLNGVGTKAVNALSSEFRVVSYREGHAFEAKFVRGLRVDSREFETREKT
                     GTLVEFVPDEQIFGEYTFVFEFIEKRLWSYACLNPGLKLSINGKEFQTEKGLQELLST
                     ELGGRALYDIGWYRGDHIEFAFTHTREYGEEHFSFVNGQYTSAGGTHLSAFREGFLKA
                     INEFFQASYHGEDVREGLAAAISIRIQNPIFESQTKNKLGNSDVRPWILQEVKKGVDE
                     ILRRNPQAAKILQEKILTNEKLRTELNIVRKEAKEAAKRIELKIPNLKDCKIHLGDGD
                     LEDQSTIFITEGLSAAGSMVSARNVYTQAIFSLRGKPENMFERPRSAIYKNGELYNLM
                     MALGIENNVENLRFARIVIATDADYDGFHIRNLLLTFFLTFFEELVVQGRVYLLETPL
                     FRVRTKKETIYCYSIHERDDGIKKLGPGSEVTRFKGLGEISPAEFGQFIGKNMRLVKV
                     DIQTLSSVPELLNFFMGKNTPQRRQFIMDNLVLDL"
     gene            8483..>9558
                     /gene="larA"
                     /locus_tag="PLQ30_06045"
     CDS             8483..>9558
                     /gene="larA"
                     /locus_tag="PLQ30_06045"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HPW02042.1"
                     /translation="MHFELKYGDGSLPLDIEQSRILSVVLPNKRKSLGDARNAVARAL
                     AQPEGTAPLLEMLIDRKPKKLVIIVNDITRPTPYSILMPPLIEVIEKAGIPDENVTIL
                     IATGIHDLHTHEQNLIVYGEEMCRRFRIISHDANDSSMLSHKGKLPSGYDYWLNRIVD
                     EADFLITIGVVMPHYFAGFSGGRKSILPGVVGKETVERNHARMVEIMDNLPPIRENPI
                     SLEMIYAARIAGVDFILNVVVDECESIVEVVAGDLEKAWYKAVDVSQAMYMIPIPEQA
                     DITICSASGFPRDINLYQAQKALDHADRATKRGGTIILVAECRNDYGEKVFERFMTAG
                     YSPEEIMSHIKAHFVIGGHKAYGFA"
BASE COUNT         2218 a         2215 c         2426 g         2699 t
ORIGIN      
        1 gtgcggatct gcctgcgccg agtctccggc aggcaggcac gcacaggcag gccaggttgg
       61 cccagatgcc tgccgcgccg cctttggcgc aggcgaggat gttcgagcct ttgagtttgc
      121 ttgttctgcc tttgtagttt gtcatttgcg atcgccttta tccttctgcg aggtggtccg
      181 cctcggcttt ttgggtcccg gtaatttctt tttctcctgc cccgtcagtt gcctggtctt
      241 ctccacaaag cgatcaaaat cgctgctggg tgcggtggcc tcgagttttc ggcgttcggc
      301 ttcgtattta tcgaactggg catgggcgtg ctcttcggcc agggtacgcg aaatcatgcc
      361 ggcatgggtc ataatattcc gctcgttgaa cctaagaaag gcatccagtt ttttcaccca
      421 atctttcatg tacatgggct tgcggcggcg agcctgatcc tcggcatagt caagatacat
      481 ggttacgatg cggttgagtt cgcggatctc ctcttccgtt aggtaattct tggccacggt
      541 gacatcggtc ttgcggatgg ggccgtaggg cgcgtttttc cacgtggtca atcccatacg
      601 gggcttgttc gcatcagccc gatgatagac gatctccgcc gcggtgtgac catggatggc
      661 ccagtgcagt ttgttctgca cggttgcgta aaacatctgg gtgatctcgg cctgagggtc
      721 gtagtcaatg ctcgtcgcgt agatatcggt gatcttctgg tagaacagcc gttcagatgc
      781 gcggatatcg cggatgcgtt cgagcagttc gtcgaaatag gactcgccga gagtgcggcc
      841 ttccttgagc cggtcatcgt cgagcgtaaa acccttgacc agcagttcgg acagccgggc
      901 ggtagcccac tggcgaaagg cggtgccgcg ggccgagcgg acacggtagc caactgcaag
      961 gatggcgtcg aggttgtagt ggtctaccat gcgggaaacc tgccgggcgc cctcggtttg
     1021 aactatccgg aatttccgga tagttgcaga agaatccagt tcttgttcgg cgtatatgtt
     1081 cacgaggtgt tcgtttacgg ttttgaccga cacctggtac agttcagcca tgagccgctg
     1141 agttagccag acggtttggc cgtcgatgcg gacttgcacc tgcagggcgc cgtcctgata
     1201 gatcagtagt tggccctccg gaggattggg gacgatctca tttgccatac ccaatgtcct
     1261 ccaggttagc ccagatgagt tggtccagtt tcttcgcctg ctcggtctgt tcgcgcaagg
     1321 cggccgtcag cttggccatt ttctcctcga acggctcacc gtcgtcttcg acctcggctg
     1381 caccgacata gcggccaggg gtgagaatgt aattatgccc gcgtatgtcg tcggtcgttg
     1441 cggatttgca gaaaccgggg atatcctcgt aggcgcgaac ctgtgtgttc gcctgcgcat
     1501 cggggctgac acgcaggtct gcccctacat tatcgccgcg ccatgcgtgg taggtgtcgg
     1561 ctatcttgct gatgtcgtcg taggtcagtt ctcgatgaac gcggtcgatc agggtgccca
     1621 gcttgcgggc gtcgatgaag agcgtctcgc cgcgtcgatc ccggaatcgg ctgttctttt
     1681 tatcgcgggc gatgaaccag aggcagaccg gaatttgggt tgaatagaat agctggccgg
     1741 ggagagcgac catgcaatct accaggtcgg cttcgatgat gcttttgcgg atctcgccct
     1801 cgccagcttg attggaggac atggaaccgt tggccaggac gaagcctgcc agaccggtcg
     1861 gggccaggtg gtagataaag tgctgcaccc acgcgaagtt ggcgtttccg gctggcggga
     1921 tgccgaactt ccatcgtttg tcgtctttga ggcgctcgcc accccagtcg ctgtcgttga
     1981 agggcggatt ggccaggaca tagtcagcct tgagatcggg gtgcagatcg cggtgaaagc
     2041 tgtcggcgtg ttccttgccg aggttcccgt cgatgccgcg aatagccagg ttcatctttg
     2101 ctagccgcca ggtggtgtgg ttggattcct ggccgtagat gctgatgtcg ccgatcctgc
     2161 cgccgtgggc ttcaacaaac ttctccgact ggacgaacat tccaccggaa ccgcagcagg
     2221 ggtcatatac acggccttta taaggtctca gcatttcaac gaggagctga accacgcaac
     2281 ggggcgtgta gaactgaccg ccctttttcc cctcggcgcc ggcgaactgc gaaaggaagt
     2341 actcgtagac acggccgaga atatctttgg agcggtttgc cttatcgccc aggccgatgt
     2401 cgccaatcag gtcgatgagc tggccgaggc gctgcttatc gaggcgcggg tgggcatagt
     2461 ctttgggaag cacgcccttg agtgaggggt tgtcgcgctc tatggcgagc atggcgtcgt
     2521 cgaccaattt gccgatggtc ggttgcctgg cgttggcctt gagatgagac cagcgggctt
     2581 cttttggaac ccagaatatg ttgacggccc ggtactcgtc ggggtcttct gggtcggctc
     2641 cctcggagcg atccgcctcg agcttgattc gatgttcctc gaaggcatcg gagatgtact
     2701 tgagaaagat caggccgagc acgacgtgct tatactcagc agcatccatg ttcgagcgca
     2761 gcgcgtcggc ggctcgccag agttcggttt cgaagccaag gttcgctccg ctgttgttct
     2821 ctttggccat ttcagatctc ctcatcggta aagaattccc gcaaaaggtc cttgccccga
     2881 tcacccagac cccgtagtaa gtcctcgatc tgcttcaacg ccagtggaga cggcttggca
     2941 cggccgtttt cccaccggtt gatggttggg taggtcacgc cgagcttggc tgcaaatttt
     3001 tcctgggtga gccccgttcg ttggcggagt tcacggacta atcgaggtat gtcggcctga
     3061 gcattcaaca tcgttctcct ttcagatcga acagggacct gttcgaaaca aagattatat
     3121 catgcgcctc aacagatgat atatcatata ataaagagga gcgatgtcaa tatagatttt
     3181 tgccgaggga tttaagaggt ggaaagaatt acggtttcag cgctaaacta cgccggttag
     3241 ttccttgaag ctcccggtca ggtgatgtcc cccacacact gggggccgag cccgctccga
     3301 aaggtcttcc ttccgaatcg ggagcaaggc gagtcggctt ccggtgtcag ggtgaggccg
     3361 ctcaaaagac cttagcggat gctgccttgt gagcttccaa gtgagcttcc aaaaggcgaa
     3421 tcggatatga tgtgaaaggt ggcctaagtg ggcgtggctg tatgataaac aacgagaccc
     3481 catgggatca tacccgtggg gtcttaagtt aactaagcac cgtcgaaatc gacggattta
     3541 attttagctc ccccgcggtg gttcgaacac cggacccagt ggttaacagc cacttgctct
     3601 gccagctgag ctacagggga atatgaattc tcggcggata gtaaaatagc caaagcaaga
     3661 gcttttgtca aggtttcgca ttgccttgtc atttaagaat caaaagttcg aagtctctta
     3721 ggagccagag atcaaaaatg ggctatcgat tcttagctga caaatcaaat tgattgtcta
     3781 tatatgatag atgcaggtct atgcttgacc tctgtatatg gaaagggtaa aaatggtcaa
     3841 aagggttctg tccatgcata gagtcaaaat tgccattacc gcctatgagc cggtcttata
     3901 tacagagttc tatcccatct ttgaagacag ccctttcctg ataattatcg atgaatacaa
     3961 ccatgtccaa aagtattctg cagagatcgg ctcaaaagag attctaaagg gtagggcaga
     4021 gtggataatt ggcagagggg caaggattct cataacagga tctatcgaca acgaggatta
     4081 ttggaaactg cggctggctg gtgtaactat aagatgggaa tcttttggcg aggtaaaatc
     4141 cttggtagaa agagcaagaa tagctgcaga ccatgtgcag gaaacaatgg aaaatagaaa
     4201 acctatagac aggagcagat atagcaaagg cctgagggct aatagctttg cagccccatg
     4261 ttttgtttac tttcgagaag aagatatcaa atatctagaa aggttggaac aaaagattga
     4321 aaaagaaggc aaaaagctgc ttttacagat caaagatgag gaaggctcca attatgatga
     4381 ttatgagacc agtggaaata ctacttctaa aaaatgacaa gttgattcag cgttcttaat
     4441 tttctttctt ctgctttttt aaagcagctt tgagcaagcc ttctgaaatt gtctccaaag
     4501 gaggaatttc ctttttcgat cttgcttccc tatttggctt tttcttggag atatgtgccg
     4561 tggaagcatc gaggtcttct tgcttgcgtt tgttatctgg ctcctgggtg gtaagtattt
     4621 caaaacgggt tatttctcta tccgtgagct ttattccctg tgcaccccta tttctcaccg
     4681 tatagttttt ggcatgaaac acttcatagg tttttttgct tcttgattta ggtttgtatt
     4741 ccagtttgaa gtcaaattcg ctcgcagttg aaaatcctag aatcaccgct gtctccggta
     4801 acagactata gtctctgccg gtaagccagc tgtctattat agttctcttt atatatgccc
     4861 cgttttcgcc ggataatgaa tagatcagcg ttattgtcgt tttagagagg ctctcctttt
     4921 tcgctatcgc agtccataac aacttttgtc ctgcaaagaa cttatccgga agagggataa
     4981 ttgtatatat tccgttgttt ctcaaaatga ggattttgtc gaatttcgag gcttccaaga
     5041 gtttttcgcc cgaaacctct gttcccagat aacctccttc agcgtcatat tttagcgcaa
     5101 aatcatgccg cgctacggct ctggcttcta ttctcttgaa tgctctcaat ttggttttgc
     5161 gcgggcagat tttttctatc atctcgatat agctctgcag tatatcaatt gcataatcta
     5221 caatatgacg caaatgcatt tcattctcgg atatccttcg ttcgatctgc tccatttctc
     5281 tcttggcttt ttctatatca tagagagata tcctgcgaat gggtatcttg aggagtcgct
     5341 ccacatcttc cacttcgact tttcgagaaa gctcttttgt aaatggcttt agtccgtcta
     5401 gtaccgcctt gataacggta tcattggtcc ttttggtttc gatcgatgta tagattttct
     5461 cttcgataaa aatgcgttcg agcgttctag aaaagagttc attctttaac tcgtctcttt
     5521 ctagttcgag ctcttggcgc agcaggtcaa gtagtcttgc cgcatgatat tttatggtct
     5581 cggatacagt ggtaacgaca ggctttcctt ctttgataac caggcagttg caggagatcg
     5641 atcgctcgca ttctgtaaag gcatagagcg cgtctaccac atcccgtgcg tatactcctc
     5701 ttgccagctt tagctctatt tcgactttgc tggtcgtgaa atcggagatg gaagcgattt
     5761 tgagatgacc agccctgaca gcttcttcaa tgctatttat gaggctttcg gtagtggaac
     5821 cataaggaat ctcttttata acgatttttt ttgggtctga gctatcaatg gtagcgcgaa
     5881 ctctgacttt cccatttcca tctgcatatt cgctgcaatc tgcagttcct cctgccggaa
     5941 agtccggaga aaggctaaag gcctttccct ggagatatgc aatctcggcc ttgattactt
     6001 cgatgggatt gtgcggcagt atccttgtgc tcattcccac cgcaatacct tctgctccca
     6061 agatcaaaat aacaggaatt tttgcaggaa atacgagtgg ttctttattt cttccatcat
     6121 agctatctat atagcgagta atcttaggat gatagaaaat ctctttggca aatggagaac
     6181 atttgcattc tatataacgg ggggcggagg cttcgtctcc agttatgggg tttccgaaat
     6241 tcccctgtgt atctagaaat aagcctctgt tcgcaaggtt tatgagagca gaagcgatag
     6301 aagcgtctcc atgggggtgg tacttcatgc aatgcccaac aacatttgcg actttatgga
     6361 atttgccatc gtccatttca aagagggagt gcagaattcg acgctgtact ggcttaaggc
     6421 catcgtccag ttccggaatt gccctgtcca taattacata agaggcatag tagagaaagt
     6481 tttcggttag caacttattt atataggcca ctctattgtc tatcctttca tagcttttcc
     6541 ggtagattcc tggttttcaa aacctcaaag atctaatacc aggttgtcca ttatgaattg
     6601 tcgcctctgt ggtgtatttt ttcccataaa gaaatttagg agctccggaa cagatgataa
     6661 ggtctgaata tctaccttta ctaatcgcat gtttttccca atgaattgtc cgaattccgc
     6721 gggacttatc tcgcctaagc ctttgaaccg tgttacttct gatcctggac cgagtttctt
     6781 tattccgtcg tctcgttcat gaattgaata gcagtatata gtctcttttt ttgtccttac
     6841 tctaaaaagg ggtgtctcga gcagatagac tcgtccctga acgaccaatt cttcgaagaa
     6901 ggtcaaaaag aatgtcagaa ggagatttcg tatgtgaaaa ccgtcataat cagcgtccgt
     6961 ggcgataacg atgcgagcaa agcgcaaatt ctcgacatta ttctcgatgc caagggccat
     7021 catgagattg tagagctctc cattcttata gatcgcagat ctagggcgct cgaacatatt
     7081 ctctggttta ccgcgtaaac taaaaattgc ttgagtgtat acgttccggg cgctcaccat
     7141 gcttccggct gcggagaggc cttcggtaat gaatatcgtc gattgatctt ccaggtctcc
     7201 gtcgccaagg tgtatcttgc agtccttgag gtttggtatt ttgagttcaa ttctctttgc
     7261 ggcttctttt gcctcttttc gaacgatgtt cagttcggtg cgcagttttt cgttggtaag
     7321 aatcttttcc tggagaattt tcgccgcctg ggggttgcga cgcagtattt catctacgcc
     7381 ttttttgact tcctgtagta tccatgggcg gacatcgcta ttgccgagct tgtttttggt
     7441 ctgactttcg aatatcggat tctgtattct aatggaaata gcggccgcaa ggccttctcg
     7501 gacatcttcg ccatggtacg atgcctgaaa gaactcgttt attgccttta gaaagccttc
     7561 gcggaaggcg gagaggtggg ttcctccagc cgaggtatat tgaccattta caaaagagaa
     7621 atgctcttct ccatattccc tggtatgggt gaaagcaaat tcaatatgat ctcctctata
     7681 ccagccaatg tcgtagagag ctctaccacc gagctctgtc gacaatagct cctgaagccc
     7741 tttttcggtt tgaaattctt taccattgat agatagttta aggccagggt taagacaggc
     7801 atagctccaa agacgctttt cgatgaattc aaatacaaag gtgtattctc cgaagatttg
     7861 ttcatctggg acgaattcta cgagtgttcc tgtcttctcg cgagtctcga attctcggga
     7921 atcaacgcgc aagccccgaa cgaatttggc ttcaaaagca tgtccttctc gataagaaac
     7981 aaccctgaat tcgcttgata atgcatttac tgcctttgta cctacaccgt tcaatccgac
     8041 agagaattga aatacatcgt cattgtattt tgcgccagtg tttatcaccg atacacattc
     8101 gatgaccttc cctaaaggta tgccgcgtcc ataatctctt acgcgcattc ttctgccctg
     8161 gaggttgatt acgatttggt tgccggcgcc catgatgaac tcgtctactg agttgtcaat
     8221 tatctccttg agtagaatgt atattccgtc gtctgggctc aggccatcac ccagcctccc
     8281 aatatacatg ccagttcgca gccttatgtg ttccagcgag gacaatgttt tgatttttga
     8341 ttcatcgtat gtcgatttat tgccagtcac gctaggtatt ctatcatgat ggacaataga
     8401 gggtatagag tattttgtct tcgacgatta tatgatagac ctttgattat atagaaagaa
     8461 attcaaatat aaggcggtaa tcatgcattt cgagctaaag tatggagatg gaagtcttcc
     8521 actggacatc gaacaatcaa ggattctcag tgtcgtgctt cccaataaaa gaaaatcact
     8581 tggtgatgct cgcaatgctg ttgcaagagc gcttgcacaa cctgaaggca cagcacctct
     8641 tttggagatg ttaatagacc gcaagcccaa gaagcttgta atcattgtca acgacattac
     8701 gagacccacc ccttattcga tcttgatgcc tcctcttatc gaagtgatag aaaaagctgg
     8761 gattcccgat gaaaatgtga caattctgat tgctacaggg attcacgatc ttcatactca
     8821 tgaacagaat cttattgtat atggcgaaga gatgtgtcgt agattcagga ttatttcgca
     8881 tgatgcaaac gattcctcaa tgctatcgca caaaggcaag ctgccttcgg gatacgacta
     8941 ttggctgaat aggattgtgg atgaagcgga ttttcttatc acgatcggtg ttgtcatgcc
     9001 gcattatttt gcaggattct caggcggcag aaagtccatc ttgccaggtg ttgtcggaaa
     9061 ggagactgta gaaagaaacc atgcgcgtat ggttgaaatc atggataatc ttcctccgat
     9121 cagggagaat ccgataagct tggaaatgat ctatgcagcc cgaattgcgg gcgtggattt
     9181 cattttgaat gttgtggtgg atgagtgcga gagcatagtc gaagtagttg ccggcgatct
     9241 tgagaaggcc tggtataagg cagtagatgt gagccaagcg atgtatatga tacctattcc
     9301 tgagcaggcg gatatcacga tttgtagtgc ttcaggattt cccagggata tcaatctata
     9361 tcaagctcag aaagccctgg atcatgccga ccgtgccaca aagaggggag ggactatcat
     9421 acttgttgcg gagtgtcgca atgactatgg agagaaagtg ttcgagagat tcatgactgc
     9481 aggctattca ccagaagaga tcatgtcgca catcaaagcg cattttgtca taggtggaca
     9541 taaagcctat ggttttgc
//
LOCUS       DAOMZL010000065         9538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_6793_length_9538_cov_15.539703, whole genome shotgun sequence.
ACCESSION   DAOMZL010000065 DAOMZL010000000
VERSION     DAOMZL010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6793_length_9538_cov_15.539703"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..972)
                     /gene="amrS"
                     /locus_tag="PLQ30_06050"
     CDS             complement(100..972)
                     /gene="amrS"
                     /locus_tag="PLQ30_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPW02043.1"
                     /translation="MTYNRLSADKARYWRSLDNSRIQCALCAHACIIQDGSKGHCSAR
                     QNRSGKMILPFYAWISSAAIDPIEKKPLRHFMPGTSTYSVGFWNCTMDCPFCQNWEIA
                     HPQPAYIQEEIIEPDRLIELAMASGCPSISFTYSEPLLHIEYVIEAMQIAHSRGLKTI
                     LVTNGNALDEPARHILAHTDAANIDLKTADPLIYRRLLGGELKVVQNFIRVSVQLCHI
                     EITSLAVPGVLDSSAQIEEISSFIASLSQEIPLHITRYHPAWKYTKAPLSPETMHEMT
                     ESARKYLKYVYSYW"
     gene            complement(972..1379)
                     /locus_tag="PLQ30_06055"
     CDS             complement(972..1379)
                     /locus_tag="PLQ30_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02044.1"
                     /translation="MSISSYFDAEDLEMLEDVSDIVKYREHPAFDAVPFVGSIKKHPY
                     DKEKFLILLLHEQKNMAWFKEGEIIEVKTRDVQALDELPSTIDLNGTAISTFRIWIRR
                     RAIAVRFEPFEVNDKEYRPFDTHSISRLLSKGD"
     gene            complement(1410..3116)
                     /gene="lnt"
                     /locus_tag="PLQ30_06060"
     CDS             complement(1410..3116)
                     /gene="lnt"
                     /locus_tag="PLQ30_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPW02045.1"
                     /translation="MNELVKGLYRQNMPRLGNLKNNSTPFWIRLLWAFLAAIFAALAQ
                     PNEIFLYGNWFIGIFCLVPLYMALVDTEKLGEASLIGALFGGLYHALTSYWLFFYKDF
                     AFWTLGTTTIAYAVIYGVALMYGCFLLHHTDGCRPLVFALAWAALEFAKSTGFLGYPW
                     GLLAYSFTGLPIMLQTADLWGVYGISAFLAFCSSATSELILQKTRKMDSVRSCIRWPE
                     ITAATWALILLLYGALIMSRPFPIKGTLRLLLCQQNTDPWTSGENEALYANIRVAKQA
                     LKSIQEEKSAKPDLIVFSETSLRRPYKEFKSWFEENPPEEPLLPFLRIADIPLLTGLP
                     VVWNWETYEASNSVGIIYPTGRMGETYAKIHPVPFAEAIPFWEFSWFRSFFQNVIGLE
                     SGWVMGREEVIFTLSSKDKYHSFDEMGSLPTIRFATPICFEDAFSELCRNFIHRGADL
                     LINLTNDSWSRTESAQIQHYAVARLRAIETRRALVRSTNSGVTCVVGADGKNIAELAQ
                     FITTSMVVDVPIYESHFTFYLIFGDWFAWACTAFVGLLFIWAILDTKRKNYVIQKDIN
                     HL"
     gene            3214..3711
                     /gene="coaD"
                     /locus_tag="PLQ30_06065"
     CDS             3214..3711
                     /gene="coaD"
                     /locus_tag="PLQ30_06065"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPW02046.1"
                     /translation="MPNAVFAGSFDPPTSGHLDVIESALSIFDGLYVVLAINPEKDGL
                     FTIDERIEMLNAMNRFGQRMKVCKWDGLVTDFASQHGCTVLVRGLRNAAELPYESTMA
                     YMNQRLDPSIKTVFFLYDAEHVDISSTLVRDLVSHSRLPNDIVPEEVEMVLRQILQRR
                     GQPLS"
     gene            3831..4016
                     /gene="rpmF"
                     /locus_tag="PLQ30_06070"
     CDS             3831..4016
                     /gene="rpmF"
                     /locus_tag="PLQ30_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPW02047.1"
                     /translation="MAVPKFRTSKARTRRRRSINMKLNPPAVVHCSNCGNPVMLHRVC
                     PKCGFYRGRQVITPESK"
     gene            4028..4270
                     /gene="acpP"
                     /locus_tag="PLQ30_06075"
     CDS             4028..4270
                     /gene="acpP"
                     /locus_tag="PLQ30_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPW02048.1"
                     /translation="MDEQELFEKIKKIIADKLEVDESKITMDASFRQDLGADSLDTYE
                     LVYGIEEELGIQIPDEKANEFETVKDAYEFIKTQVK"
     gene            4284..5093
                     /gene="rnc"
                     /locus_tag="PLQ30_06080"
     CDS             4284..5093
                     /gene="rnc"
                     /locus_tag="PLQ30_06080"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPW02049.1"
                     /translation="MRRKDHLPSNNRLEISSSRRSALLRFQRATDISFHDITLLDIAL
                     THSSSVNEKSPYIQDNERLEFLGDSVLNLCISHILYESLPDNDEGELAHMKNVLVSET
                     SLASIAISLGIGEFLILGKGEEMSGGRRKPAILADATEALLGAYYLDSGIQSTKEIVS
                     RIFSSKIDELRKTPSKDFKSIIQEYAQKRGLEIPSYRVARIEGPDHARHFFVSCVIDG
                     ETFGPMEGMSKKEAEQRVAQHIFEVLHKRDAETARLLDIVTKGSTEIDEKI"
     gene            complement(5106..6479)
                     /locus_tag="PLQ30_06085"
     CDS             complement(5106..6479)
                     /locus_tag="PLQ30_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPW02050.1"
                     /translation="MIIPSIDPEVRRIADRIIQSGKKAFLVGGAVRDAFLRKPVMDYD
                     IATDATPEECMSLFPFAIPTGIKHGTITIVPRSRRYKVEITTLRKEAAYSDGRHPDSV
                     TFVDDITQDLSRRDFTINAMAIDLMCNAFIDPFDGEKDLKDKIIRAVGNPMERFQEDG
                     LRTVRAIRFSAQLEFAIEPNTLVAIAKCSDTLSKISTERIRDEFSKILLTAKPSVGLR
                     LLSDTNLIKEIIPELEICKGISQPDHGEQDVYNHLLSTVDAVIPDNLSEDKVLILRLS
                     ALFHDIGKPACYRAENGKISFYRHEIESEKIARQILLRLKYPNRVIDKVCHLVRYHMF
                     NYEPGWTDAAIRRFVARVGFSSIPDLFTLRLADTMATTGKPISWPALAEFRERIDRIL
                     EEKQALSLKDLAINGEDLAKIGIPRAKQMGEVLSELLDNVLEDPSLNTKEKLLEIAKG
                     RYFEGFK"
     gene            6535..7176
                     /locus_tag="PLQ30_06090"
     CDS             6535..7176
                     /locus_tag="PLQ30_06090"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HPW02051.1"
                     /translation="MKALIVTGGDNPPSSIIWCLSKKADIVIAADAGLEVCKIANIEP
                     HLAVGDFDSVDPAILSQVSPEKILRFPEEKDRSDTELAIEAAHRYGAKYIILAGAGAG
                     RLDHLLAVRALFERNDPIKEWHTAHESVFLVARGLRLQASMSINSIVSVFPVSGGASG
                     MLSKGLKWPLDGLKWKRGEFGLSNKTISEEIEIYSGENSILVILPLGSKVSVS"
     gene            complement(7171..7659)
                     /locus_tag="PLQ30_06095"
     CDS             complement(7171..7659)
                     /locus_tag="PLQ30_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPW02052.1"
                     /translation="MEYLFNFNKIGYLQGRRPKGCILCLVRDDSKEVEKLVVDETGHF
                     IVSINLYPYNPGHLIVFPKRHIVDIREYTDDEERELDRLVKTCLKALDKFGSPSAYNI
                     GYNMGLSAGASIEHLHMHIIPRYPREIGIVELIAGSRVLVQDPKDTLLQLREIFGNLA
                     IL"
     gene            8170..>9538
                     /gene="gltX"
                     /locus_tag="PLQ30_06100"
     CDS             8170..>9538
                     /gene="gltX"
                     /locus_tag="PLQ30_06100"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPW02053.1"
                     /translation="MSVRVRYAPSPTGNQHIGGVRTALINYLFAKSQGGTFILRLEDT
                     DRTRYSQEYVQNLYDTFRWLGFYWDEGPDVGGPKGPYVQSERIDLYRRYAEELVLMGK
                     AYYCFCDSERLERLRLEQISQKKDEIGYDRHCRFITEEERAKRLAEATPYVIRLKIPL
                     HGVTVFHDALLGQIEWKNEDISPDPVLLKSDGFPTYHLANVIDDHLMGITNIMRAQEW
                     IPSAPMHKIMYDAFGWEMPELCHLPMVLGKDGHKLSKRHGATAVKEFRKAGYLPEALI
                     NYIALLGCSYEDGRDLYSLDELVRLFKIDRLNKAPAVFDYQKLEWFNGQYIRMKSDKE
                     LAELIRPYLIDAGLRTEEDPQKDRMELAAIPLIKERLKLLGDSPAIMSYLYKRLEMPP
                     AEAFIPKRASMDEAIMYLEECRSIIQDSNLDDIPAVEERFKEFATVIGKKLGDILMPL
                     RVAITY"
BASE COUNT         2726 a         1981 c         2120 g         2711 t
ORIGIN      
        1 agatccacag tctggcactc taccacctga gctacaccca ccgtgtaatt ggcgaagatt
       61 acaatgccga aataaatcaa gactgtcaag agggattatt caccaatagg agtatacata
      121 tttcaaatat ttcctagcgc tttcagtcat ctcgtgcatg gtctccggag aaagtggcgc
      181 cttagtatat ttccaggctg gatggtatct agtgatatgg agaggtatct cctgcgaaag
      241 cgaagctata aaactggaga tctcttcgat ttgtgcggat gaatcgagaa caccgggaac
      301 ggcgagagat gtaatttcga tatgacagag ttgtacagaa actcttatga aattttgtac
      361 aacctttaat tctccgccaa gcaatcttcg atagatgaga ggatcggcgg tttttaagtc
      421 gatgttcgca gcatccgtgt gggctaggat atgtcgagct ggctcatcta gcgcatttcc
      481 atttgttacc aggatcgttt ttaaacctcg gctatgtgct atctgcattg cttctatgac
      541 atattcgatg tgcaacagag gctcggaata ggtaaagctt attgatgggc aaccagatgc
      601 catagcgagt tctatcagtc gatctggttc gatgatttct tcttgaatat atgcaggttg
      661 aggatgagca atttcccagt tctgacaaaa tgggcaatcc attgtgcagt tccagaatcc
      721 cacagaatat gtcgaggttc ctggcatgaa atgccttagg ggtttcttct cgataggatc
      781 tattgcggca gaagaaatcc atgcataaaa aggaagaatc atttttccag atcgattttg
      841 gcgcgcacta caatgccctt ttgatccatc ttgaatgatg catgcatgag cacatagagc
      901 acattgtatt cttgagttgt ctaagcttct ccagtatctc gccttatctg cggaaagtct
      961 gttgtatgtc atcaatctcc cttgctaaga agccttgata tgctgtgagt atcaaaaggt
     1021 ctgtattctt tatcattaac ttcgaacggt tcgaatcgca cggcaattgc tcgccgcctt
     1081 atccatatac gaaatgtgct gatcgcagta ccgttgaggt caatggttga gggaagctcg
     1141 tccagagcct gcacatcgcg ggtcttgacc tcgataatct cgccttcttt aaaccaagcc
     1201 atgttttttt gttcatgcag caagagtatg agaaatttct ctttatcata ggggtgtttt
     1261 tttatggagc ctacgaatgg aacagcatca aaggcgggat gttctcgata ttttacaata
     1321 tcagatacat cctcaagcat ctctaaatcc tctgcgtcaa aataactaga aatactcaag
     1381 gtaatcctcc atagtgattc tgtttaatat tataaatgat ttatgtcctt ttgtattaca
     1441 taatttttcc tttttgtatc tagaatcgcc cagataaaaa gcagcccaac gaaagcagta
     1501 caagcccagg caaaccagtc tccaaaaatc agatagaaag taaaatgaga ttcataaata
     1561 ggaacatcca ccaccataga agtggtaata aactgagcca attcagcaat attctttccg
     1621 tctgcgccta ccacacaggt gactccagag ttagtcgagc gcacaagtgc tcgccttgtc
     1681 tctattgctc taagccgtgc gacagcataa tgctgaatct gagcactctc agttcgagac
     1741 catgaatcgt ttgtcagatt tatcaacaaa tctgcgcctc gatgaatgaa atttctacat
     1801 agttcggaaa aggcatcctc gaagcatata ggcgtagcaa atctaatggt aggcaagctg
     1861 cccatttcat caaaactatg atatttatct ttggagctca gtgtaaagat tacttcttct
     1921 cttcccatta cccagccgct ttcaagtccg attacatttt gaaaaaagga gcgaaaccaa
     1981 gagaattccc agaaaggaat ggcttctgcg aaaggtacag gatgtatttt tgcataagtt
     2041 tcacccattc tacctgtcgg gtaaataata cctactgaat tggatgcctc atatgtttcc
     2101 caattccaga ccaccggaag accggtcagc aatggaatgt cagcgatccg aaggaagggc
     2161 aatagaggtt cttcgggagg attctcctca aaccaggatt tgaactcctt gtaaggccgc
     2221 ctcaagctag tttcgctgaa tactatgaga tcgggcttgg cgcttttttc ttcctgaatt
     2281 gatttcaaag cctgtttagc tactcttata ttcgcgtata gcgcttcatt ctctccagat
     2341 gtccatggat ctgtattctg ttggcagagc agaagccgca atgttccctt gataggaaaa
     2401 ggccgagaca ttatcaaagc accatagaga agtaatatca aagcccatgt agcggcggtt
     2461 atttcaggcc agcgaataca actccttacg gagtccattt ttcttgtttt ctgcaagata
     2521 agttctgagg ttgccgatga acaaaaagcc aagaaggcag aaatgccgta gacaccccag
     2581 aggtcggccg tctgcagcat aatcggaagc ccagtaaatg agtaagccaa aagtccccaa
     2641 ggatatccca aaaagccagt cgattttgca aactcgagag ctgcccatgc cagagcgaat
     2701 acaagaggcc tgcagccatc tgtgtgatgc aagagaaaac acccatacat aagggctact
     2761 ccatatatta ccgcataagc gatagttgta gtccccaggg tccaaaatgc aaagtcctta
     2821 tagaaaaaga gccaatagct tgtcagtgca tgatagagtc ctccaaatag agcaccaatc
     2881 aggcttgctt ctccgagttt ctctgtatca acaagtgcca tatagagtgg cacaaggcaa
     2941 aagatgccga taaaccaatt cccatacaag aatatctcgt tgggctgagc aagtgcggca
     3001 aatatggcgg ctaaaaatgc ccatagcaag cgaatccaaa aaggcgttga attattttta
     3061 agatttccga gccttggcat attctgtcgg tacagtcctt tgactagctc attcataatg
     3121 aaattagtat aagccaaaga tctctttggc taaagagcaa gggcaaattc tttccattga
     3181 ctgttggagc agaactgcat taaattaaag accatgccaa atgcggtctt tgcaggaagc
     3241 tttgatcctc cgacatcagg ccatctcgat gtgatcgaga gtgcccttag catattcgac
     3301 gggctttatg tcgtcttggc catcaacccc gaaaaagatg gattgttcac tattgatgag
     3361 cgtatcgaaa tgctcaatgc catgaacaga ttcggtcaac gaatgaaggt atgtaaatgg
     3421 gatggtctag tgactgattt tgctagtcaa catggatgca cagtgctcgt gcgcggactc
     3481 cgcaatgctg ctgaattgcc ttatgagtcc actatggcct acatgaacca aagactcgat
     3541 ccttccataa agacagtttt tttcctctat gatgctgaac atgtagatat aagctctaca
     3601 cttgtgcgtg atcttgtatc gcatagtagg cttcctaatg atatcgtacc agaagaagtt
     3661 gaaatggttc ttaggcagat attacagaga agagggcaac ccctttcata acttgcgcaa
     3721 ataatatatg tttaacgact gcgagcatgt ctatcttgac atttttataa attttgtata
     3781 atttggcatg caattatcat gccgatattg aataattatg aggttctacc atggctgtac
     3841 cgaaatttag gacgtctaaa gcaaggacga gaagacgccg ttccatcaac atgaaactca
     3901 acccaccagc tgtcgtacat tgcagtaatt gtggaaaccc agttatgttg catagggttt
     3961 gtccaaaatg cggtttttac aggggtcggc aagtaattac ccctgaatct aaataaggag
     4021 tcttccaatg gatgagcaag agctatttga gaaaatcaag aagattattg ctgacaagct
     4081 cgaagtagat gaaagcaaga tcacaatgga tgcaagtttc cgccaggatc ttggtgcaga
     4141 ttctctagac acctatgagc tcgtatatgg tatcgaagag gaactgggta ttcagattcc
     4201 agatgagaag gcgaatgaat ttgaaaccgt aaaagatgcc tatgagttca taaagaccca
     4261 ggtaaaataa gacattctaa ggcgtgagac gaaaagatca cttaccatca aacaacaggc
     4321 tggagatttc gtcttcacgc cgcagtgctc tactgcgctt ccagagagcc acagatattt
     4381 cttttcatga tataacactc cttgatatag cccttacgca cagctcctcc gtaaacgaaa
     4441 aatctccata tatacaggac aatgaacgct tagaattcct gggtgatagt gttttaaatc
     4501 tatgcatatc tcatatctta tatgaaagtt tgcctgacaa tgatgaaggc gaattagccc
     4561 atatgaagaa tgtcttggta tcggaaactt ctctagcatc aatcgcaata tctctcggta
     4621 ttggggaatt tcttatcctt gggaagggag aagagatgag tggagggcga agaaagccag
     4681 ctattctagc cgatgctaca gaagcacttc ttggtgctta ctacctggat agtggaatac
     4741 aatctaccaa ggaaattgtt tccaggatct ttagtagcaa gattgatgag ctccgaaaaa
     4801 caccaagcaa ggacttcaaa tcaataattc aagaatatgc tcaaaagcgt ggactagaaa
     4861 ttccttccta tagggttgcg agaatcgaag ggccagacca tgctcggcat ttttttgtat
     4921 cctgtgttat agatggggaa acctttggcc ctatggaagg aatgtcaaaa aaagaggccg
     4981 agcagcgagt cgctcagcat atatttgagg tattacataa acgagatgca gagactgcca
     5041 gactcttaga catcgtgaca aaaggcagca cagagatcga tgagaagata tagagagtcg
     5101 atctcttatt tgaatccttc aaaatatctg ccttttgcta tttctaggag cttttctttg
     5161 gtgttcaaag atgggtcttc gagcacatta tctaagagct cggatagaac ttctcccatt
     5221 tgtttggcgc gcggaatccc gatttttgcg agatcttctc catttattgc cagatctttg
     5281 agcgaaagag cctgtttttc ttccagaatt cgatctattc tctctcggaa ttctgctaga
     5341 gcaggccagg atatgggttt ccctgtagtt gccattgtat cggctaatct aagcgtaaac
     5401 agatcgggaa tcgaggagaa tcctacgcga gcgacaaatc tacgtatcgc tgcatctgtc
     5461 caacctggtt cataattgaa catatgataa cgcacaaggt gacagacctt atcgataact
     5521 cgatttggat acttgagtcg tagaagaatt tgccttgcaa ttttctctga ttctatttca
     5581 tgtcgataaa agcttatttt cccattttca gctcgataac atgcaggttt gccaatatcg
     5641 tgaaaaaggg ctgaaagacg aaggataagg accttgtctt cgcttagatt atctggaatg
     5701 acagcatcca cggtcgaaag caggtgatta tagacatcct gttctccatg atcaggttga
     5761 gaaattcctt tgcatatttc taattccggg attatttcct ttatcaggtt cgtatccgaa
     5821 agaagtcgaa ggccaaccga aggctttgca gtcaataaga tcttggaaaa ctcatcgcga
     5881 attctctctg tggaaatttt cgagagagtg tccgaacact tagctatggc aacaagggta
     5941 ttgggttcaa tggcaaactc gagttgagca gaaaagcgta ttgcgcgtac tgttctaaga
     6001 ccatcctcct gaaatcgttc cattggattg cccactgcgc gaataatttt atctttcagg
     6061 tccttttctc cgtcaaatgg atcaatgaat gcattgcaca tcagatcgat ggccatcgcg
     6121 ttaatggtga aatcccttct tgagaggtcc tgggttatgt catcgacaaa ggtgacgcta
     6181 tcggggtgtc tgccatcaga ataggcagct tcttttcgaa gcgttgttat ttccacctta
     6241 tatctacgtg aacgaggaac aatagttatg gtgccatgtt ttataccggt aggtatggca
     6301 aaagggaata aactcatgca ttcttcgggt gttgcatcgg tcgcgatatc gtaatccata
     6361 acgggctttc taagaaatgc atcgcggaca gcaccaccga ccaggaatgc ctttttacca
     6421 ctttggatta ttctgtctgc gattctgcga acctctggat cgatactagg gattatcata
     6481 tcttcctttg gagtgtatct taggcttcag ctttatgtct atgaggaaac tgatatgaaa
     6541 gctcttattg taactggtgg cgataatcct ccctcatcca taatttggtg cctttccaag
     6601 aaagccgata tcgtaatagc cgcagatgcc ggacttgagg tatgcaaaat agccaatata
     6661 gagcctcatc tagctgtggg agattttgac agtgtggatc cagcaatact atctcaggtt
     6721 tcaccggaga agattcttcg ttttccggaa gaaaaggatc gttctgatac cgagctagca
     6781 atcgaagcag cgcatcgtta cggagcaaaa tacataatcc tcgccggagc tggagcaggg
     6841 cgtcttgatc atctgctcgc agttagagct cttttcgagc gcaacgaccc tatcaaagag
     6901 tggcatacag cacacgagag tgtttttctc gttgctcgag ggcttaggct gcaggcctca
     6961 atgtcaatta atagtatcgt ttctgtcttt cctgtctccg gaggagcctc tggcatgcta
     7021 tctaaaggac tcaaatggcc cttagatggc ttgaagtgga agcgcggcga atttggccta
     7081 agcaacaaga caatctccga agaaatagag atttattctg gtgagaattc aattctcgta
     7141 attctacctc ttggttccaa agtgtctgtc tcatagaatt gctagattcc cgaatatttc
     7201 tcttaactgc aggagggtat cttttggatc ctggactagg acgcgagatc cagctatgag
     7261 ctccacaatg cctatttctc gcggataacg aggtataata tgcatgtgta aatgttctat
     7321 gctggctcct gcagataagc ccatattgta tccaatgtta taggcagagg ggctcccgaa
     7381 tttatcgagc gcttttagac atgttttgac taatctatct agttctctct cttcgtcatc
     7441 agtgtattcc cttatatcca caatatgtct tttgggaaag actatcagat gccctggatt
     7501 ataagggtaa agattaatgg atacaatgaa atgcccagtc tcatctacta cgagtttttc
     7561 cacttcttta gaatcgtcgc ggacaaggca gagtatacag ccttttggtc ttctaccttg
     7621 tagataacca attttgttga agttaaagag atattccata tggaagtatg atatattagc
     7681 attatggaaa acaaaagcgg ctcgcaatta caatccgctt ttataccgac tcttaagcgg
     7741 tttctagatt ataccgtggt ttttagtatc atctttttga taagtagttt cgttgtgctt
     7801 ttctttggcg gaattagtga atatgaatgg atttggcgat caatatttga aatatgcaat
     7861 atgcttcttc taagtgtgag tctcttcaat atgacaagca tggtaggctt aaggatagct
     7921 gggaaggatc gtttctctat tgggcgtttc attcttcagc tccttctctt tgtttttgca
     7981 gtagcagctc taatgctgaa tatcttcgta aaaatcaccc gaatctgttg aattttggtt
     8041 gccagatgga ttttgcgact attggagctg attgtcttta attcttgcat agaaagagat
     8101 ggaaatgtat agtaattcaa atctatgcaa tgttaaggaa ttttcagcag agattgatca
     8161 ggaggaataa tgtccgttag agtgcgctat gccccatccc ctacaggaaa tcaacatata
     8221 ggcggcgtaa gaacagcgtt gatcaactat ctattcgcga aatcccaagg tggaactttt
     8281 attttgcgct tagaggatac ggatcgaacc cgttattcac aggaatatgt tcaaaatcta
     8341 tacgatacat tcaggtggct tgggttttac tgggacgaag gtcctgatgt cggtggccct
     8401 aagggaccct atgtgcaatc tgaacgaata gatctctatc gaaggtatgc agaagaactt
     8461 gtccttatgg ggaaggctta ttattgtttc tgcgattcag agcgcctcga gagacttcgt
     8521 ctggagcaga tctcacagaa aaaagacgaa ataggctacg atcgccattg tcgttttatc
     8581 acagaagaag aaagagcaaa aaggctcgcg gaagcaacac cttatgtgat caggctaaaa
     8641 atcccccttc atggggtaac ggttttccac gatgctcttc ttggtcaaat agaatggaaa
     8701 aatgaggata taagcccaga cccggttcta ttaaagtccg acggttttcc gacctatcat
     8761 ctggctaatg tgatagatga tcatttgatg ggaattacaa acattatgcg tgctcaggag
     8821 tggattcctt cggctcctat gcacaagatc atgtatgatg cctttggttg ggaaatgcct
     8881 gaactctgtc atctgcctat ggtactgggt aaagacggtc acaagctatc caagcgacat
     8941 ggggctacgg cagtgaagga atttcggaaa gctggctatc ttcctgaagc actcatcaat
     9001 tatatcgctc tgcttgggtg ttcgtatgaa gatggtcggg atttatatag tctggacgag
     9061 cttgttcgtc tattcaaaat agacagactc aataaggcac ctgcagtatt cgattatcaa
     9121 aaactggagt ggttcaatgg tcaatatatc cgcatgaaaa gcgataaaga gctagccgag
     9181 cttattcgtc catatttgat cgatgctggc ttgagaactg aagaagaccc acaaaaggac
     9241 cggatggagc tggccgcaat accattaatc aaagagagac taaagctgct tggagattcg
     9301 cctgcgatca tgtcctatct ctataaacga ctggaaatgc ctccggcaga ggcatttatt
     9361 cccaagagag ccagcatgga cgaagcaata atgtatcttg aggaatgcag atcgatcatt
     9421 caggattcta atctcgacga cattcccgcc gttgaggaga gatttaaaga atttgctaca
     9481 gtgattggaa agaaacttgg agacattctg atgcctctaa gggttgcgat cacttatt
//
LOCUS       DAOMZL010000066         9439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_6883_length_9439_cov_13.740409, whole genome shotgun sequence.
ACCESSION   DAOMZL010000066 DAOMZL010000000
VERSION     DAOMZL010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 9439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9439
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6883_length_9439_cov_13.740409"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..434)
                     /gene="cas2e"
                     /locus_tag="PLQ30_06105"
     CDS             complement(48..434)
                     /gene="cas2e"
                     /locus_tag="PLQ30_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HPW02054.1"
                     /translation="MLVIVTESIPDRLRGYLSRLMLEIRAGVFVGNYSVRVREMLINT
                     IYANVEDGNVIVAWSANNESGFDFETIGKNRRIPVQFDGTKLISFLPEKEILDKENTS
                     SLTHLGKDNHLSQNSVDFDKLSNSFS"
     gene            complement(415..1326)
                     /gene="cas1e"
                     /locus_tag="PLQ30_06110"
     CDS             complement(415..1326)
                     /gene="cas1e"
                     /locus_tag="PLQ30_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HPW02055.1"
                     /translation="MRIPSSPISIKERTSMLFLQYGLLDVQDGAFVLVDENGVRIQIP
                     IGGVACLFLEPGTTITHAAVSLAAQVGTLLVWVGEGGVRLYASGQPGGARSDKLLYQA
                     KLALDNELRLKVVRAMYQYRFKEMPPSKRSIEQLRGIEGVRVRTMYELLAQKYRVPWK
                     NRRYDPDNWDNADPINKTLSAATHALYGICEAAILAAGYAPAIGFIHTGKPLSFVYDI
                     ADLVKFDTVVPIAFEVIAKKSTNPVRDVRLRCRDSFRESKLLKMLIPTIEQILAAGEI
                     QPPEAPPESVPIAIPNKEGISDAGHRY"
     gene            complement(1336..2055)
                     /gene="cas5e"
                     /locus_tag="PLQ30_06115"
     CDS             complement(1336..2055)
                     /gene="cas5e"
                     /locus_tag="PLQ30_06115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HPW02056.1"
                     /translation="MSSEIGYLALRLQGPLQSWGYSSHFNRRSTSTMPTKSAIAGICC
                     AAFGYERGSPQEKQFISGFALLSMIAISIPRRIEHQNKQYKWYQILQVNRLQDYHTVQ
                     NTRRADGSINDDCVVTHRSYITDAIFGVVLSGNKEFLEEIAGALEDPVWGLWLGRKSC
                     LPSAPILAGNAVHRGGVKNTQEEALSLLIGDKDLNSYSYQEEVGTFLEGSDSIPDSPL
                     CFDSKARQFTPRRIKSHEAIK"
     gene            complement(2045..3082)
                     /gene="cas7e"
                     /locus_tag="PLQ30_06120"
     CDS             complement(2045..3082)
                     /gene="cas7e"
                     /locus_tag="PLQ30_06120"
                     /inference="COORDINATES: protein motif:HMM:NF020902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HPW02057.1"
                     /translation="MRHLELHIIQSVPVACLNRDDLNSPKTALFGGVQRARVSSQSWK
                     RAIREYAKEKAPSYFKGERTRLLYDPLYNAMIDNGLSEEDADIGAKALIDYLVKIDEK
                     SKDRVKSTTLYFLSPLEIATLSKEYAKTKDPKKAAKSINAEALCDAADISLFGRMVAN
                     DPSLKVEGAAMFSHALSTHKADNEIDFFAAVDDLQSDEEAGAGMTSTQEFNSATYYRF
                     MAINLDMLADSCHLAALSVDERKLVVRTFIEATIMAMPGARKNAMNANTLPAYVLGIV
                     RSNGHPIQLVNAFEKPVWSKEGYLAKSIQALKEEYARQKDIWGIKDIYTKAIPEVSLQ
                     QFLDEVVSYVI"
     gene            complement(3102..3755)
                     /gene="cas6e"
                     /locus_tag="PLQ30_06125"
     CDS             complement(3102..3755)
                     /gene="cas6e"
                     /locus_tag="PLQ30_06125"
                     /inference="COORDINATES: protein motif:HMM:NF020377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HPW02058.1"
                     /translation="MSWLTKIEIDASLAYKNKFIDSYTWHQALWQCFPGRSSQKRSFL
                     TRVDETSHGFIAWILSLDKPIRPSWCPEEGWAIKEISPGFLNHRWYAFNLKANPTKAI
                     MQKPAVVAPAIDGQIRLTRGKRVPLTKREDLKAWLERKAAQGGFKIVETIPLDIGPMA
                     ESYFSRGEQKAYHGSIQFRGVLEVTNKEVFEKTYYSGIGSAKAFGYGLLLLAPVSLN"
     gene            complement(3739..4263)
                     /locus_tag="PLQ30_06130"
     CDS             complement(3739..4263)
                     /locus_tag="PLQ30_06130"
                     /inference="COORDINATES: protein motif:HMM:NF021034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HPW02059.1"
                     /translation="MSSLIDQLRWYKEHNDRGFFANLRSYLIESKRQRAWPALYRLNV
                     PIEDEAQSYIAAWYSMHPEETQEGNLGTTCRIIQNKTQGKQSTDNNLTPMERRFLQLL
                     ASGDERELRNRITRIILMAKSQQVPVNYEQLKIDSNNWNQKTKISWAQAFWAANATLP
                     GKQEEKRRSNELAH"
     gene            complement(4247..5836)
                     /gene="casA"
                     /locus_tag="PLQ30_06135"
     CDS             complement(4247..5836)
                     /gene="casA"
                     /locus_tag="PLQ30_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HPW02060.1"
                     /translation="MNVATDPWIPVVTVKGEPILVSLMQVFTEGEKFADLAVRPYERV
                     SLLRLFLCIAHAALDGPKDYEEWLSLPSRLSKSASQYLGTWHDSFELFDLQKPWLQAA
                     GISTEADTPTPWNDLSKWTSTSKLCLTYASGHNPTLFDHEGMQEKRSITIEMAILSLL
                     SYQCFSPGGLMPQVFWHEKQTTKSSRDSPCISESMLHAIIRGGNILTTIYLNMVPYEY
                     ILHFYEDIGRPLWEMIPTSFYDEPNVQNATKTYLGRLVPLSRLVLLHQQEPYMLLGNA
                     LEYPTFSNGFAQEVTATVVRTKIRNTEKRILLSYRPTKSIWRQLPAILVKDKSFEVGG
                     PLSLYNLRENQGFDLQVCALAHNKGSLLDVFESVIAIPAQLDSDIGRNTYDAEIKRSE
                     HIADILGKAVEMYRKKLDAAWERRLQNAGPQKGALRAQLYDRAANFFWTAIEQNINLL
                     ITFIQALDTENAEHHKELWRHFLTSQALAAYNFACSNDTPRQILAFTEGWQFLNDTLI
                     ALDILPNQQYKQTEEGDEQSY"
     gene            complement(5833..8388)
                     /gene="cas3"
                     /locus_tag="PLQ30_06140"
     CDS             complement(5833..8388)
                     /gene="cas3"
                     /locus_tag="PLQ30_06140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HPW02061.1"
                     /translation="MGTIHFWAKTTENGAPGISVYQHMINVGCVAQCIAEIQKDLLES
                     FRLRASEVGALAALHDIGKISPGFQRKCMAWLKENNLLKIESEGNWQDEMEPDHGKIS
                     YVIIQSFLRSTGLSTKESAYLASAVASHHGKLIPPDDRPYQLRGRITEKRSGITWDEE
                     QQKSAKAIWQQFNADSNHLKLDDNLTAYWYLAGLTSVADWIGSDERFFSAEYKSNNEA
                     FDSHEVVRQALDSIGYVQPHIKEGLSFYDMFGFYPNPMQQKTMETITGPGIYVIEAPM
                     GQGKTEAALAAAYQLMLKGLAQGIYFALPTQTTSNRIHLRIKDFIQKISPDAGPTRLI
                     HANSWLLTDDILPQIREKSGRDWFASAKRALIAPFGVGTVDQALLGILAAKHFFVRQF
                     ALAGKVVIIDEVHSYDLYTGTLIDALIRELLSLQCTIIVLSATLTNKRRAQLLNSSDS
                     TSPNIGVIMDKKPQPYPLISGRVKERLIDIQEIQSPPSRNVKIIWIAKEAAILKALEL
                     AQKGGAVLWICDTVESAQTTYLQLKDIVAPTTKYGLLHSRFPFYRREILEEEWMQRLG
                     KEGVYRCGCILVATQIVEQSVDVDADLIITELAPTDMLLQRMGRLWRHERSTRPVTEP
                     EFCLLQEEKSLTTFKELEARSIKNTLGGKAFIYAPYILMRSLNLWDSIKEITIPNDIR
                     SLLESTYEELEEEPSAWKELCDEWFGSDSAKKMLAMMNANLWQIPLSDEEGVQTRVNE
                     QPTIDVVLCSHTQPSRYMLIDGTEVALDNSEYSLSVARNLHKNIVKVPQYCFYEAYRP
                     PPIFSKYLHGWAAVGIVSDDTTVQIDGLKEMYQLCYSNELGLKVNNNKKEENL"
     gene            complement(8543..9208)
                     /locus_tag="PLQ30_06145"
     CDS             complement(8543..9208)
                     /locus_tag="PLQ30_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02062.1"
                     /translation="MKKCFLIMTLCIAAFSAYAQSNKPYDDLVAALKQAATEQDDSAR
                     LALYDAIVHSLGVVTIGQTAESPSIQTSSKWIFDQEVDPLTDNKKYFFMLKADSGKND
                     YGDLPVLVIRQDGDELELYINWHTYLGNDTDDYKYEAKYITMRVDQDQPTTLLWSNST
                     DSKASFCPYTYTRELVQRLGSASQFVARCTPYGDSPITAVFDVRGLKEISMPYNDRLK
                     WWE"
BASE COUNT         2511 a         2114 c         1945 g         2869 t
ORIGIN      
        1 cggttcatcc ccgcatgcgc ggggaacact cttcccttat attgctgcta agaaaatgaa
       61 ttagacaact tatcaaaatc taccgaattt tgagacaagt ggttatcctt ccctaaatgt
      121 gtcaaagaac ttgtgttctc tttatctaga atttcttttt ccgggagaaa agagataagc
      181 tttgttccat cgaactgaac cggaatgcgc ctattctttc ctatggtctc aaaatcaaag
      241 ccactttcat tatttgccga ccaggcaact attacatttc catcctcgac atttgcgtaa
      301 atggtattta tgagcatttc tctcactcgt acagaataat tgcctacaaa cacacctgcc
      361 ctgatttcaa gcataaggcg gcttaaatag ccacgcaatc gatctgggat tgactcagta
      421 acgatgacca gcatcgctta tgccttcttt attcggaatt gctatcggaa ctgattctgg
      481 tggggcttca ggtggctgaa tctcccctgc tgccaaaatt tgctcaatag tgggaatgag
      541 catctttagt agtttcgatt ctctaaaact atcgcgacat ctgagacgaa catctctcac
      601 agggtttgtg cttttctttg caattacttc aaaagcaatc ggcacaacgg tatcaaattt
      661 tactaaatcg gcgatatcat agacaaaaga aagaggctta ccggtatgta tgaaaccgat
      721 cgcaggagcg tatcctgccg ccaaaatcgc tgcttcacag atcccataaa gcgcatgcgt
      781 tgcagcgctg agagtcttat ttataggatc ggcattgtcc cagttatcag gatcatatct
      841 ccgatttttc caaggtacgc gatatttttg tgcaaggagc tcatacatag tcctgactcg
      901 caccccttca atacctcgaa gttgttctat gcttcttttg cttggtggca tttccttaaa
      961 ccgatattgg tacattgccc ggactacttt aagccgtaat tcattatcta atgcaagctt
     1021 agcctgatag agtagtttat cggacctggc acctccaggc tgccctgatg catacagcct
     1081 gacgcctcct tctcctaccc atacaagaag cgttcctacc tgagcagcaa gactcactgc
     1141 agcatgggtt attgtggtac cgggttcaag gaataaacaa gcaactccac caataggaat
     1201 ttgaattctt acaccatttt catcaacgag cacaaaggct ccatcttgta catcaagaag
     1261 cccatactgt agaaaaagca ttgaagtgcg ttcttttatt gagatggggg aagaaggaat
     1321 gcgcattgct tttccttatt tgattgcttc atggctcttt attctgcgtg gtgtaaattg
     1381 tctagccttt gaatcaaagc agagaggact atcgggaatg gaatcgcttc cttcaagaaa
     1441 tgtccctact tcttcctgat agctatagga attcaagtct ttatcaccga taagcaaaga
     1501 aagcgcttcc tcttgagtat tttttactcc tccacgatga actgcattgc ccgcgagtat
     1561 tggcgcagaa ggcaagcacg actttctgcc tagccataag ccccacacag gatcttcaag
     1621 agcgcccgca atctcctcaa gaaattcttt gttgccagaa agaaccacac caaaaatggc
     1681 atctgttata taagaacggt gcgtcactac gcaatcatca ttgatgcttc catcagctcg
     1741 ccgcgtattt tgtacagtgt gataatcctg cagcctattg acttgtagaa tttgatacca
     1801 tttatactgc ttattttgat gctctatgcg acgtggaatc gatattgcaa tcatgctaag
     1861 taatgcaaag cctgagataa actgcttttc ctgcggactt cctcgctcat agccgaatgc
     1921 tgcgcaacaa atccctgcaa ttgcgctttt tgtcggcatt gtgcttgtac tacgcctatt
     1981 gaaatgactg ctatagcccc acgattgcaa aggaccctgt aagcgcaaag cgagatagcc
     2041 aatttcagat gacatatgaa acaacctcgt ctaagaattg ttggaggctt acctccggaa
     2101 tagctttagt ataaatatct tttatgcccc aaatatcttt ttgtcgcgca tattcttcct
     2161 taagcgcttg gatggatttt gcaagataac cttctttgga ccacactggc ttctcaaagg
     2221 catttacaag ttggatagga tggccattgg agcgcacaat gcccaaaaca tatgcaggga
     2281 gtgtattagc attcatggca ttttttcgcg ctcctggcat tgccatgata gtggcttcga
     2341 taaaagtgcg caccactagt tttcgttcat caacggataa agcagctaga tggcagctat
     2401 cggcaagcat atccaagttt atcgccatga agcggtagta ggttgcagaa ttgaattctt
     2461 gtgtacttgt cataccagcg ccagcttctt catcagattg taaatcatcg actgcggcga
     2521 agaaatcgat ctcattgtct gccttgtggg ttgatagagc atgtgaaaac attgcagctc
     2581 cttcaacttt caatgaaggg tcattcgcga ccatcctgcc aaaaagcgaa atatcggcgg
     2641 catcgcacag tgcttcagca ttgatcgatt ttgcggcttt ctttgggtct tttgtttttg
     2701 catattcttt ggaaagggtt gctatctcta agggggataa aaaatagagt gttgttgatt
     2761 taaccctgtc ttttgatttt tcatctattt tgacaagata atcgatgagg gcctttgctc
     2821 ctatatctgc atcttcctct gataatccat tatctatcat tgcattatac agtggatcgt
     2881 ataataagcg tgtcctttcg cccttaaaat atgatggagc cttttctttt gcatattctc
     2941 gaatagctcg cttccacgat tggctcgata cacgtgctcg ctgtactcct ccaaaaaggg
     3001 cagtcttggg agaattaaga tcatctcgat ttagacatgc caccggtacc gattgaatga
     3061 tatgcaattc caaatgtctc attttctatc tccttttttc atcaattgag gctcacagga
     3121 gccaaaagta aaaggccata tccaaaggct ttggcactac caataccgct ataataggtt
     3181 ttttcaaata cctccttgtt agtgacttct aaaactccac ggaattgaat gcttccatga
     3241 tacgcttttt gttcccctcg cgaaaaatag gattccgcca taggaccaat atcgagagga
     3301 attgtttcga cgatcttgaa tcctccttgc gctgcctttc tttccaacca agctttaaga
     3361 tcttctcttt ttgttaaagg gactcttttg ccacgagtca atctaatttg tccatcgata
     3421 gcaggagcaa cgacagcagg cttttgcatt atagctttcg ttggattagc tttgagatta
     3481 aatgcatacc agcgatgatt gagaaaacct ggactaattt cttttattgc ccatccttct
     3541 tcaggacacc aggaagggcg aataggctta tcgaggctca atatccatgc tataaaccca
     3601 tgagaagtct catctactct tgttaaaaaa ctgcgttttt gagaacttct gccaggaaag
     3661 cattgccata atgcctggtg ccaagtatag gaatcaataa atttgttttt gtatgcaaga
     3721 gatgcatcaa tttcgatttt agtgagccag ctcattactc ctcctctttt cctcttgttt
     3781 gccaggaagt gtagcattag ctgcccaaaa ggcctgtgcc caactgatct tagttttttg
     3841 attccaattg ttactatcaa ttttaagttg ttcatagttt acgggcactt gctggctttt
     3901 tgccattagt atgatgcggg taatacgatt gcgtaattca cgctcatctc cggatgctaa
     3961 aagctggaga aagcggcgtt ccatcggcgt caggttatta tctgtacttt gctttccctg
     4021 cgttttgttc tgaataattc tgcatgttgt accaaggttc ccttcctgag tttcctctgg
     4081 atgcattgaa taccacgctg caatatagct ttgcgcctca tcttctattg gtacattgag
     4141 tcgatagagc gctggccacg cacgttgccg tttgctttca atgagatagc tgcgcaaatt
     4201 ggcaaagaat cctcgatcgt tatgctcttt ataccatcgt agttggtcaa taagactgct
     4261 catcgccttc ctccgtttgt ttatattgtt gattcggtaa tatatctagc gcaataagcg
     4321 tgtcattcag gaattgccat ccttcagtaa atgcaagaat ttggcgtggc gtatcattgc
     4381 tgcaagcaaa attgtatgct gctaaggctt gcgaagtaag aaaatgccgc cacaattctt
     4441 tatgatgctc tgcattttct gtatctaaag cttggataaa tgtaataagc aaatttatat
     4501 tttgttctat tgctgtccag aaaaaatttg ctgctcgatc ataaagctgt gctcttaatg
     4561 cccctttttg agggcctgcg ttttgtaggc gcctttccca cgctgcatca agttttttac
     4621 gatacatttc gaccgcttta ccaagtatgt cagcaatgtg ttctgatcgc tttatttctg
     4681 cgtcataggt attcctgcct atatcagaat ccaattgtgc aggaatagcg atgacagatt
     4741 caaacacatc aaggagcgag cctttattat gagcaagcgc gcagacctgt aaatcaaaac
     4801 cttgattctc tcttaaatta taaaggctaa ggggaccacc aacctcgaag gatttgtctt
     4861 ttactaggat agctggaagt tgccgccaga tgctctttgt tggccgatac gaaagcagaa
     4921 tccgtttctc agtattgcga attttggtgc gcacaacggt tgcagtaact tcttgtgcaa
     4981 atccattact aaacgtggga tactccaagg cattccctaa taacatatat ggctcttgct
     5041 gatgcaagag cacaagacgg gaaagaggaa caagtctgcc caggtaagtc tttgttgcat
     5101 tctgcacatt gggttcatca taaaaagaag tgggtatcat ctcccaaagt ggcctgccaa
     5161 tgtcttcata gaaatgaaga atatattcat agggaaccat atttagataa atagttgtaa
     5221 gaatattgcc accgcgtatg atagcatgca gcatggattc agaaatacaa gggctatctc
     5281 gtgacgactt agtagtttgc ttttcatgcc aaaacacttg gggcatcaag cctcctggag
     5341 aaaaacattg gtagctaaga agagaaagaa ttgccatttc tatagtaatc gagcgttttt
     5401 cctgcatgcc ttcatgatca aaaagtgtag gattatgtcc tgatgcataa gtcaagcaga
     5461 gctttgaggt tgaagtccat tttgaaagat cgttccaagg agttggcgta tcggcttctg
     5521 tagaaattcc agctgcttgc aaccatggtt tttgcagatc aaatagttcg aatgagtcgt
     5581 gccaagttcc gagatactgt gaagctgatt ttgaaagtcg actgggaagg ctgagccatt
     5641 cttcataatc ttttggtcca tcgagtgcag cgtgagcaat gcagaggaaa agccgcaaaa
     5701 gagaaacgcg ctcgtatggc cgaacagcaa gatcggcaaa tttttctcct tctgtaaaga
     5761 cctgcattag gcttactaga ataggctctc ccttaacggt aacgacagga atccatggat
     5821 ctgtggcaac attcatagat tttcctcttt tttattgttg ttcactttaa gaccaagttc
     5881 gttgctatag caaagttgat acatttcttt tagtccatca atctgaactg tggtatcgtc
     5941 agacacgatg ccaacagcag cccaaccatg aagatatttt gaaaatattg gaggaggccg
     6001 atatgcttca taaaaacaat attgaggcac ttttacgata ttcttatgca agttgcgcgc
     6061 taccgagagc gaatactctg aattgtctaa agcaacttca gtgccatcaa taagcatata
     6121 tcgactaggt tgagtatgag agcataggac tacatctatg gttggttgtt cattgacgcg
     6181 cgtttgcacc ccttcttcat cgctcaaagg aatctgccat aaattcgcat tcatcatggc
     6241 gagcattttc tttgctgaat cggagccaaa ccattcatcg cataactcct tccatgcaga
     6301 aggctcttct tcaagttctt cataagtaga ttcgagcaag gatcgtatat cattaggaat
     6361 tgttatttcc ttaattgagt cccaaagatt taaagagcgc ataagaatgt agggagcata
     6421 gatgaaagct tttcctccta gagtattttt tattgagcgt gcttctaatt ccttaaaggt
     6481 tgtaaggctc ttttcttcct gaagaaggca gaattctggc tcagttacag gacgtgtgct
     6541 tctttcatgc ctccataaac ggcccatccg ctgcaacaac atatctgtag gcgccagttc
     6601 tgtgataata aggtctgcat cgacatcgac gctttgctcg acaatctggg ttgcaacaag
     6661 gatacagccg cagcgataca cgccttcttt acctagtctt tgcatccact cttcttctaa
     6721 aatttctcgt cgatagaaag ggaatcttga atgcaatagc ccatattttg ttgtaggcgc
     6781 cacaatatcc tttagctgaa gataagtagt ctgtgcactc tctaccgtgt cgcatatcca
     6841 cagcacggct ccaccttttt gcgctagctc caaggctttt aatattgcag cttctttagc
     6901 tatccatata attttaacgt tccgcgatgg aggtgactga atctcttgta tgtcgataag
     6961 tctttctttg actcgacctg aaataagtgg ataaggttgg ggctttttat ccattattac
     7021 tccgatatta ggagatgtag aatctgaaga atttaaaagc tgcgcacgtc gtttattcgt
     7081 caatgttgcc gagaggacaa tgattgtaca ctgaaggctt aatagctcgc gaatgagtgc
     7141 atcaatcaga gtacccgtat aaagatcgta actatgtact tcatcgataa ttacaacctt
     7201 tcctgcgagt gcaaactgcc gcacaaaaaa atgcttggcc gcaagaatgc ctaaaagtgc
     7261 ctggtccaca gttcccacgc caaatggcgc aatcagtgcc ctttttgccg aggcaaacca
     7321 gtcgcgaccc gatttttccc ttatctgcgg tagtatatca tccgtaagaa gccatgaatt
     7381 agcatgaatc aaacgcgtag gacctgcgtc agggctaatc ttctgtataa aatccttaat
     7441 gcggagatga atccgattgc tcgtcgtctg agtaggcaaa gcaaaataaa ttccttgtgc
     7501 gagccccttc agcattagct ggtaggctgc tgctaacgct gcttcagttt tgccctgtcc
     7561 cataggggcc tcgatgacat agataccagg accggtgata gtctccatcg ttttctgttg
     7621 catcggattg ggataaaaac caaacatatc gtaaaaagaa aggccttctt tgatatgagg
     7681 ttgtacatag ccgatagaat caagcgcttg tcgaacaact tcatgggagt caaaagcctc
     7741 attattactc ttgtattcag cagaaaaaaa tcgttcatca gagccaatcc aatctgcaac
     7801 tgaagtaagc cctgccagat accaataagc tgtcaaatta tcatcgagtt ttaaatggtt
     7861 gctatcagca ttgaattgtt gccagatcgc ttttgcactt ttttgctgct cttcatccca
     7921 tgtaattcca ctccgttttt ctgttatccg accacgaagt tggtatggcc tatcatctgg
     7981 cggtataagt ttgccatgat gggaagctac tgcactcgca agataggcgc tttctttggt
     8041 tgaaaggcct gttgaacgca agaaggattg aataatcaca taggatatct ttccatgatc
     8101 aggttccatt tcatcttgcc aattgccctc gctctctatt ttgagaaggt tattttcctt
     8161 tagccacgcc atgcattttc tctgaaagcc tggcgaaatc ttgccaatat catggagcgc
     8221 agccaatgcc ccgacttcgg aagcgcgcaa cctaaaagat tcaagcaaat ctttttggat
     8281 ttctgcgatg cattgagcta cacagccaac attgatcatg tgttgataga cagaaatacc
     8341 aggtgctcca ttctccgttg tttttgccca aaaatggatt gttcccattt tatgctttac
     8401 cagttttgta cttataacac gatgcaaaca taaccaaagt gatcaaggtt tttgttctcc
     8461 tcgttctatc attttcaaaa accattctat cacatctatt ttctagctca agacttgatt
     8521 catttggtgt taaagtgtcc cttcactccc accactttaa ccgatcatta taaggcattg
     8581 aaatttcctt tagaccgcga acatcaaaaa ccgcggtaat ggggctgtcg ccatagggtg
     8641 tgcagcgcgc gacgaactgc gatgcactcc caagtcgttg taccagctca cgcgtgtatg
     8701 tgtaggggca gaatgaggcc ttgctatcgg tggaattgct ccaaagcagg gtggttggct
     8761 gatcttgatc cacgcgcatg gtgatgtatt tcgcttcata tttataatca tctgtatcat
     8821 tgccaaggta ggtatgccag ttaatgtaga gctcaagttc atctccgtcc tggcgtataa
     8881 cgagtactgg caggtcccca taatcgttct tgccactatc agctttcagc ataaagaaat
     8941 atttcttatt atcggtgaga ggatcgactt cttgatcaaa aatccacttt gatgaagtct
     9001 gaatcgaagg actttcggca gtttggccaa tagtcactac accaaggcta tggacaatgg
     9061 catcgtataa ggcaagtctt gcgctatcgt cttgctctgt agcagcctgc ttgagagcag
     9121 ctacaagatc atcgtatggc ttgttggatt gggcatacgc cgaaaaggct gctatgcata
     9181 atgtcatgat caaaaagcat tttttcatat cgatcctctt gttgtgaata attattttat
     9241 gacaagaata gggacatatg atgtcccgca tgacttcttt tttaattttt ctctaaaatt
     9301 cttcatttct cctttgctct ctccaccatc ctttttatct cttcttgcca cagcttgcga
     9361 aactccggcg gtccaagcac ttcgcagttg gcgccgcagc gaagggctcg gccaaggatt
     9421 tcggtccagt cgtggacgg
//
LOCUS       DAOMZL010000067         9233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_7076_length_9233_cov_10.670407, whole genome shotgun sequence.
ACCESSION   DAOMZL010000067 DAOMZL010000000
VERSION     DAOMZL010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 9233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9233
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7076_length_9233_cov_10.670407"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /gene="coaE"
                     /locus_tag="PLQ30_06150"
     CDS             complement(<1..374)
                     /gene="coaE"
                     /locus_tag="PLQ30_06150"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPW02063.1"
                     /translation="MFILGLTGGYCAGKNEVAIILEKCGWKLTDVDELGHAALPLVCD
                     ELVDAFGSSILKEDGSIDRKRLGSLVFNDKDQLKKLENIVHPAMLSLLDKEIEKAQQN
                     IIEKLCINAALLYRFPQLDSCDA"
     gene            complement(392..3247)
                     /gene="polA"
                     /locus_tag="PLQ30_06155"
     CDS             complement(392..3247)
                     /gene="polA"
                     /locus_tag="PLQ30_06155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPW02064.1"
                     /translation="MDNKSPLYILDVYAIIYRSYFAFIRKPLRNSKGENVSALFGFFR
                     FLFSLFEQRKPELFAAALDSIGPTFRHTQFATYKATRQKTPEDLTAQIPRIETILQAL
                     EVPAIRSEGYEADDIIATLADQCRKEGRPCFIVSGDKDLLQLVGDDILALRPSENFTF
                     RELDAQGVFDEWGVRPDQIRDFLALTGDASDNVPGVRGIGEKTAQKLISMFGSLEAIY
                     DNLPAIEPESLRKKLEEGRESAFLSRELISLCYDVPNCPHDVAVLKPSLNIEAAAPLL
                     LREDIKSLIPDELRKRLHAKPIAGEEVASAESLQRISGQVRVAPSTNSAISLLAGSPG
                     AIQQASSMAPLESISGLSIGNPMQVKYEPVLSIERLDRIIDEAIARSVCAFDTETDSL
                     DVHKAGLVGFSLAFDADSAWYVPLKSPDAKCIPKDLALSSLSRLMKEQNMLLVGHNLK
                     FDINVLSTAGLEANLPCFDTMIAAWVLDAEAPSFSLSSVASRFLGISGLEYGDVVPKG
                     LTFADVPLATATQYASEDSHFSFLLYQMLSKEIETQSLHNIFYNIEMPLLPILAEMER
                     IGIRVDAEHLRAYGEELQIELSNIESQIFHLVGHEFNLASPKQLADVLFSERKLPVQK
                     RTKTGFSTDTSVLEELAPLDPVPQLILRQRLLTKLKTTYIEPLAELAMAQGRIHTTFM
                     QTGAATGRLSSRDPNLQNIPIREEEGRKIREAFIAEQGHLLISADYSQIELAVMAHLS
                     KDPNLIKAFKEGVDIHKRTASFIFGISEDQVDAERRRIAKTINFGVIYGMSAFRLARD
                     LGISNSLAKQFIDSYFSTYSGVADFIQKTIKEAQERGMTYTLFGRRRKILGINSKNKT
                     EQQAAQRVAVNTPIQGTAADIVKIAMIRVHRRLKKEMPEVHMLLQVHDELVFEAPKAM
                     VEGAMKLIKEEMEHAVLLDIPLRVSMESAASWGQMHL"
     gene            complement(3269..3748)
                     /locus_tag="PLQ30_06160"
     CDS             complement(3269..3748)
                     /locus_tag="PLQ30_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPW02065.1"
                     /translation="MDQIFDRLDMLVRAWMSSTFGEGFGRTRFRDSEMFEDQDLADAW
                     DELESYLDPFKADKKHKTRTNWKSYSYRYYDTGETLEEETKRKAVEEAYIFLGLEPYA
                     PFAQVKMRYKKLLKEHHPDRHNKSDSDTAEANAISAKINAAYQLIETWEESQAKSFK"
     gene            3822..4946
                     /locus_tag="PLQ30_06165"
     CDS             3822..4946
                     /locus_tag="PLQ30_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPW02066.1"
                     /translation="MNTNIHRKSYCVVIGGLNLDIQAFCYSEYQPGDSNPGQIHRSPG
                     GVGRNIAENLLRLGLDVELMTMVGDSSSWDRLISRTEKLGISLAHSPRIKKAPLSVYL
                     CILGRDGDLVGAVADMRAMEKMQIHQLQARKFLLDSAEAIVVDGNIPQDCIEWIAMNY
                     GRLEYEDKQHISCINKGSRDSSASHRPLLIADPVSTKKAEKFISSFGKFDLAKPNINE
                     AAKIAGLEQNASIEAIALSLREKSLLPKELYISQGGQGMSFVDKEIIEHIPLSSSENC
                     PHVINRSGAGDAACAALAWISLLERRIGKNCKDSPLSISPRDKAKIALSSAIYASSSN
                     RPVNPHLDQRNLFETTRICYPETSELINELINAIQMENHK"
     gene            4943..5845
                     /locus_tag="PLQ30_06170"
     CDS             4943..5845
                     /locus_tag="PLQ30_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HPW02067.1"
                     /translation="MNKYLDISEEVTDAISVGMPVVALESTIITHGMPWPQNLETALK
                     VENAVREEGAVPATIAIINGKLKVGLTNKELVSLANGSLKPIKASRRDFPALIAFQGN
                     GSTTVAGTMIIAEMAAIHVFATGGIGGVHRGAEKSWDVSADLEELAHTDVAVVCAGAK
                     AILDLPKTMEYLETKGVPVIGYGTSELPAFYTQHSGVRLSLRADTPQEIAMILRAKWE
                     SGLKGGVVIANPIPSQYSMSETTIEMAIQKAIAEAEQKGLKGAEVTPYLLSRVADLTG
                     GDSLEANIALVLNNARLAAKIAKA"
     gene            5986..6765
                     /locus_tag="PLQ30_06175"
     CDS             5986..6765
                     /locus_tag="PLQ30_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPW02068.1"
                     /translation="MRIIRCLYEDTIYFGYIEDDLVILSKDLPRIEIHNHEKKVWFER
                     EVAKVPFAKVFLEAPCVPTKAVCVGLNYRDHAEEFGLKIPSSPVLFIKPSTSLLSPGK
                     PIHYPTLSRQVDYEAELVVVMGKSAKNVDAKHAFDYVLGYTCGNDVTARDLQPKDGQW
                     TVAKSFDTFMPLGPWIETDLDPSSLKLRAYLNDEPKQFSSTSNLIFDVPSLIEYISQI
                     MTLNPGDVIMTGTPSGVGPMQKGDHVCVEIEGIGRLENTVE"
     gene            6779..7693
                     /locus_tag="PLQ30_06180"
     CDS             6779..7693
                     /locus_tag="PLQ30_06180"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPW02069.1"
                     /translation="MALSRILSIFLLIAVGYCARKAKLLDENGIRTVSNLVLTIALPC
                     TIISSFDRSIPFSAARDLIKVAIYAIAIHVISIAISEVLFSRMERTKKKILIYAAVFS
                     NCGFMGFPLAESIFGHSGLMYVSIYVMIFNIFTWSYGLALLSRGEKSDQRTKKIRIRD
                     MLFNPGIIAVAIGFVLWILPFSMPQTITYALALIANCTTPLSMIVVGATLASLGVKGL
                     FSGMEVWIGSLMRLLVLPTIFIILMKILRQDNFPVKVANLLVAMPAAAQTVIFAERHD
                     ADVLLASKIVFISTVLSAVTIPIVAQLL"
     gene            complement(7819..>9233)
                     /locus_tag="PLQ30_06185"
     CDS             complement(7819..>9233)
                     /locus_tag="PLQ30_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPW02070.1"
                     /translation="TTVPIVMPPLVGVLGFIFILGRGGTVNELLYQLAEGLEKSWPFM
                     SNWLYNHLPFNFIYGWHGLLLVETCHLFPFITLNVLDSLSKIDASLEEAAESVGSVGF
                     RRLFDVTIPLTIPGFITGALLVFIGAFADFAAPMVVGLTNFIAPLAYMDIQQFTDRHL
                     FKMGITVGAVMVLLSIVFLLIAKKYISLKDYSTLSYRAVERKPLKGKGAVLTLVFLIL
                     LMLICFIPYAGVALASFARSWSMSPLPTSWTMVHYEKVLLYAPGYIINTFRFCILAII
                     MCILIGVPISWIMARTQMRTRGALDILTMLVLALPGTALGIAYIRAFNTPILLATPLA
                     NSWVILPIVLAVRRLPYTVRSSYSSLLVVHHSMEEAAESVGAGGLKTFKDITLPLIWK
                     GVLAGALFSFMTSIQESAATLLLSIPGWETMTMGIFTFYQGGTHGDAAALGSILIVVG
                     VITLFAVNRLSSVAQGGLFG"
BASE COUNT         2531 a         2090 c         2099 g         2513 t
ORIGIN      
        1 gcatcgcatg aatcaagctg tggaaaacga tagagcaatg ccgcatttat gcatagtttt
       61 tctattatat tctgctgtgc tttctctatt tccttatcga gcagagagag cattgctgga
      121 tggacgatgt tttcaagctt tttcaattga tctttatcat tgaataccag cgatccaagt
      181 ctttttcgat ctatggagcc atcttctttg agtatcgagc taccaaaagc atcgacaagt
      241 tcatcgcata ccagaggaag cgccgcatgg ccgagctcat cgacatcagt cagcttccag
      301 ccacattttt ccagtatgat tgcgacttca tttttcccag cgcaatagcc tcccgttagg
      361 ccaagtatga acatcgatct ttccttttag attaaagatg catttggccc caagatgcgg
      421 ccgattccat gcttactcta agtggaatat cgagcaatac agcatgttcc atttcctctt
      481 tgatcagctt catggctcct tccaccatgg ctttcggcgc ttcaaagaca agctcatcgt
      541 gcacctgtag cagcatatgc acctcgggca tttccttctt tagccttcga tgcactctta
      601 tcatcgctat ctttactatg tctgcggctg tcccttgaat tggggtattg acggcaactc
      661 tctgtgccgc ctgctgctcg gttttgttct ttgaattgat gccgagaatt ttgcgtctgc
      721 gcccgaacag agtataagtc atccctcttt cttgagcttc cttgatcgtc ttttgaataa
      781 aatccgctac acctgaatag gtggaaaaat atgaatcgat gaattgcttt gcaaggctat
      841 tcgaaatacc cagatctcgt gccagcctga atgcgctcat gccatagatg accccgaaat
      901 tgatggtctt ggcaattctt ctgcgttctg cgtcgacctg gtcttcggat atcccaaaga
      961 taaaggaagc ggttcgctta tggatatcga ctccctcttt gaatgccttt ataagattag
     1021 gatctttgga gaggtgagcc ataactgcca gctcgatttg agaataatcg gcagagataa
     1081 gcagatgccc ctgctcggca ataaaggcct ctcgtatttt tctgccttct tcttcgcgta
     1141 tggggatatt ctggaggttt ggatctctgc tggaaagcct tccggttgct gcaccggtct
     1201 gcataaaggt agtgtgaatt cgtccttggg ccatcgccaa ttctgcaaga ggttcaatat
     1261 atgtcgtctt tagtttggtc aatagccttt gtctaagaat gagctgagga accggatcca
     1321 ggggtgcaag ctcttccagc actgaagtat cggtggaaaa gcctgttttt gttcgctttt
     1381 gtactggcag tttccgctca gaaaatagaa catcagccag ctgctttgga gaggctaaat
     1441 tgaattcatg gccaaccaga tgaaaaatct ggctttcgat attcgaaagc tctatttgca
     1501 gctcttctcc ataggcacga aggtgctctg catcgacacg aatccctatg cgttccatct
     1561 ctgcaaggat tggcaataaa ggcatctcta tattatagaa tatattatga agactctgcg
     1621 tctcaatttc cttagagagc atttgataga gaagaaaaga aaagtgggaa tcttccgatg
     1681 cgtattgagt tgcggtggcc aaaggcacat cagcaaaggt aaggcctttc ggtactacat
     1741 ccccatattc tagtccagaa atcccgagaa aacggctagc aaccgaagat agcgaaaacg
     1801 aaggagcttc agcatccaga acccatgcgg ctatcatcgt gtcaaagcag ggcaggtttg
     1861 cttccagacc tgcagtggag agcacattta tatcgaactt cagattgtgg ccaacaagga
     1921 gcatgttttg ctccttcatg agtctggaga gggaagaaag ggcaagatcc tttggaatgc
     1981 attttgcatc aggactcttt aagggaacgt accaggctga atcagcatcg aaagcaagag
     2041 aaaaaccgac aagtcctgct ttgtgcacat ctagagaatc ggtctcggtg tcaaaagcgc
     2101 agacagatcg agcaatggcc tcatcgataa tcctatcgag tctttcgatc gatagcactg
     2161 gttcgtattt cacttgcatt ggattaccga tcgataatcc tgaaatactc tcaagaggcg
     2221 ccatggaaga agcctgttgg attgctccag gagatccggc caaaagagaa attgcagaat
     2281 tcgtagaagg cgcaacccga acttgacctg aaattctctg caaactctcg gccgaagcga
     2341 cttcttctcc agctatgggt tttgcgtgta atcgctttcg caattcatcg gggataaggc
     2401 ttttgatgtc ttctcttaaa agaagaggag ctgccgcttc gatattcaat gaaggtttca
     2461 aaacagcgac gtcatgcggg caattaggaa catcatagca cagagaaata agctctctag
     2521 ataaaaatgc gctctccctt ccttcttcca gctttttgcg tagagattct ggctctatcg
     2581 caggcaaatt atcatatatg gcttcgagtg aaccgaacat cgaaatcagc ttctgagccg
     2641 ttttttctcc tatacccctt acccccggca cattatccga agcatctcct gtaagagcaa
     2701 ggaaatcgcg gatttgatcg ggtcttaccc cccactcatc gaagactcct tgggcatcaa
     2761 gctctcgaaa tgtaaagttt tccgaagggc gaagcgccaa tatatcatct cctacaagtt
     2821 gcagcaaatc cttatctccg gaaacaataa aacatggtcg accttctttc cgacattggt
     2881 ctgccaaggt tgctatgata tcgtcggcct catatccttc agatcgaatc gctggcactt
     2941 ccaatgcctg aaggatcgtc tcgattcttg gaatctgtgc ggtcagatct tccggtgttt
     3001 tctggcgagt tgccttatag gtggcaaatt gcgtatggcg aaatgtaggt cctatggaat
     3061 ctagggcagc agcaaatagt tctggtttcc tttgctcgaa tagagaaaaa agaaaacgga
     3121 aaaaaccaaa taatgctgat acattttcac ccttagagtt tcgcagaggc tttcggataa
     3181 aagcaaaata ggaacgatag ataattgcat aaacatcgag gatgtataaa ggggatttat
     3241 tatccatatt gttcttccat atgtattcct atttgaagct cttagcttga gactcttccc
     3301 atgtttcgat gagctgatag gccgcgttga tcttagcgct tatcgcattt gcttccgcgg
     3361 tatctgaatc ggatttgtta tgtcgatccg gatgatgctc ttttaagagt tttttatatc
     3421 gcatctttac ctgcgcaaaa ggagcatagg gttctaatcc caaaaaaatg taggcctctt
     3481 ccacagcctt tctcttcgtt tcctcttcga gagtctcgcc tgtatcgtaa tatcggtagc
     3541 tataagactt ccaattcgtt ctggttttat gttttttatc tgccttgaaa ggatcgagat
     3601 aagattcgag ctcatcccat gcatcggcaa gatcctgatc ttcaaacatc tctgagtctc
     3661 gaaaccttgt tcgcccaaag ccctcaccaa acgtggagga catccaggcg cgaaccagca
     3721 tatcaagcct atcgaatatt tgatccacct tatgcctcgc taaatcagat ttctgatgat
     3781 aatatagcat atttgcaaat cttggtggag ggcggaacac cttgaatact aatatacaca
     3841 ggaagtccta ctgtgttgtt attggaggct tgaatctaga tattcaggct ttttgctact
     3901 ctgaatatca gcctggtgac tcaaatcccg ggcaaattca taggagccca ggaggcgttg
     3961 gccgcaatat agcagagaac ctattgcgac ttgggttgga tgtggagctg atgactatgg
     4021 tcggagattc ttcaagctgg gataggctta tctctcgtac cgagaagttg ggcataagtc
     4081 tcgctcacag cccgcgcatc aagaaggcac ctctatctgt ctatctgtgc attctaggaa
     4141 gagatgggga ccttgtgggc gccgttgcag acatgcgtgc catggaaaaa atgcagattc
     4201 atcagcttca agctcgaaaa tttctgctcg attccgcaga agcaattgtg gtggatggaa
     4261 atattcctca ggattgtatc gaatggattg ccatgaatta tgggcgctta gagtatgaag
     4321 ataaacagca tatatcttgc ataaataaag gatccagaga ttcaagtgct tctcatagac
     4381 ctttactcat tgctgaccct gtttctacaa aaaaggcgga aaaattcatc tcgagctttg
     4441 gaaaattcga cctggcaaag ccgaatataa atgaagctgc taaaatagcc ggcctggagc
     4501 aaaatgcttc tatagaagcg atagctttgt ctctaagaga aaagtccctc ttgccaaaag
     4561 agttgtacat ttctcaagga gggcagggca tgagttttgt agataaagag attatcgaac
     4621 atattccatt gagttcctca gagaattgtc cgcatgtaat caatcgttca ggcgctggcg
     4681 atgcagcctg cgctgccctg gcctggatct cattgcttga gagacgaata ggaaaaaact
     4741 gcaaagatag cccattgtcg atttctccca gagacaaggc aaaaatagcg ctgagttctg
     4801 caatctatgc cagctcttcg aatagaccag tcaatcctca tctggatcag cgtaacctat
     4861 tcgaaaccac aagaatctgc tatcctgaaa catcagagct gataaatgag cttatcaatg
     4921 caatccaaat ggagaatcat aaatgaacaa gtatcttgat atctcagaag aagttactga
     4981 tgcaattagt gtaggaatgc ctgtagtcgc attagaatcg acgataatca cccacgggat
     5041 gccctggccg cagaacctcg agactgcgct gaaggtagaa aatgcggttc gagaagaagg
     5101 agctgtccct gctactattg cgattattaa cggcaaacta aaagtcggtc taacaaataa
     5161 agagcttgtt tcgcttgcga atggttctct taagcccatc aaagcatcac gaagggactt
     5221 tccagcgctt atagcctttc aggggaatgg ctcgaccacg gttgcgggaa ccatgattat
     5281 cgctgaaatg gcggcaatcc atgtatttgc gactggtggc atcggcggag tacatcgcgg
     5341 cgcggagaaa tcctgggatg tatcagcgga cttagaggaa ttagcccaca ccgacgtggc
     5401 ggtggtttgt gcaggcgcca aggctatact agaccttcct aagaccatgg aatacctaga
     5461 aaccaaagga gtgccggtga tcggctatgg aacatcggag cttcccgcat tctataccca
     5521 gcattctggc gtcagactct cgctcagggc tgataccccc caagagatag caatgatact
     5581 tcgcgctaaa tgggaatctg gcttgaaggg cggagttgtc atcgcaaatc ctattccttc
     5641 tcaatattcc atgagcgaaa ctacaataga gatggctatt caaaaagcga tagcagaagc
     5701 cgagcaaaaa gggttgaagg gtgccgaagt tactccatat ctgctttcaa gagttgcgga
     5761 tttgacgggc ggcgattccc tcgaagcgaa cattgcactt gtgctgaaca atgctcgtct
     5821 agcagccaaa atagccaaag catagagcgc aaaacgactt tgtacgactt tgtgcaaagc
     5881 caatcacaaa gaatataaat aattgtattt gataaatgct tatgatagga tgaatatcac
     5941 ttcgtatcca ttaataaggc ctatgcaacg ttttcggagt aaaaaatgag aatcattaga
     6001 tgcctttatg aagacaccat ctactttggc tacattgaag atgatctagt cattctttcg
     6061 aaggatcttc cgcggatcga gattcacaac catgaaaaga aggtttggtt cgaaagagaa
     6121 gtggccaagg ttccatttgc taaagtattt cttgaagccc cttgcgtccc gacaaaagca
     6181 gtatgcgttg gtctcaacta cagagatcat gccgaagagt tcgggctcaa aattccatct
     6241 tctccggttt tgttcattaa accatcgacc tcgctcttga gccccggaaa gcctatacac
     6301 tacccaacat tgtcgagaca ggtagattat gaggccgagc ttgtggtagt tatgggaaaa
     6361 tcggcaaaga acgttgatgc caaacatgcg tttgattacg tgctaggtta tacatgcggc
     6421 aatgacgtga ccgcacggga tctgcagcct aaagatggtc aatggaccgt ggctaaatcc
     6481 ttcgatacct ttatgccatt aggtccttgg atcgagaccg acctagatcc ttcatctttg
     6541 aaactaagag cttatctcaa tgatgagcct aagcagtttt catcgacctc aaacctgatt
     6601 ttcgatgttc cttccttaat tgagtatatc tctcagatta tgactctcaa tccgggagac
     6661 gtgatcatga ctggcactcc ttccggagta ggccctatgc aaaagggaga tcatgtatgc
     6721 gtcgaaatag aaggtatcgg tcgacttgag aacactgtag aataaaaagc gctcaattat
     6781 ggccttgtcg cgaattctat ccatttttct cttgatagcg gttgggtatt gcgctagaaa
     6841 agcaaagctg ctcgatgaaa atggtattag aacagtctcc aatcttgtac tcactatcgc
     6901 tcttccttgc accataattt cgagtttcga tcggagtatt ccgttttctg cagcaagaga
     6961 cctaattaaa gtagcaatat atgctattgc gattcatgtt atttctatag ctatttcgga
     7021 agtgcttttt tcaaggatgg aaagaaccaa gaagaagatc cttatctatg cggcagtgtt
     7081 ctctaattgt ggattcatgg gttttcctct tgctgagagc atttttggtc attctggtct
     7141 tatgtatgtg tctatctatg tgatgatttt caatattttc acatggagtt atggtcttgc
     7201 actattgtct cgaggtgaga agagtgacca aagaactaag aagattcgga taagagatat
     7261 gcttttcaat ccaggaatca tagcggtagc tataggcttt gttctttgga tcttgccttt
     7321 ttcgatgcct cagacaatca cctatgccct agctcttatt gccaactgta caacgccttt
     7381 atccatgatt gttgtgggag ccacattggc cagtcttggt gtaaaaggct tgttttctgg
     7441 aatggaggta tggataggat ctttgatgag gcttctggtt ttgccaacta tttttataat
     7501 tcttatgaaa atactgagac aggacaattt tccagtaaaa gtagccaacc ttttagtggc
     7561 aatgcctgca gcggctcaaa ccgtcatctt cgcagaacgc cacgatgcag atgtcttgct
     7621 cgcatcaaaa atagtcttta tttctactgt actatccgcg gttacaattc ctatagtggc
     7681 gcaattgctc taagcatgat tgattgttag gtatataatg gcataagtta taaaaactaa
     7741 ggcgaaatcc gacaaatcta acttatccac ggtcgatttt gcctttgctc aaaccctctt
     7801 tggtcaactc ggcttacctt atccgaacag cccaccttgc gcaacactac tgagtctgtt
     7861 aactgcaaat agggtaatca cgcccacgac tataaggata gagccaagcg cggctgcatc
     7921 tccatgggtg cctccctggt aaaaggtaaa tatgcccatg gtcatagtct cccaacctgg
     7981 tatcgacaaa aggagtgtag ctgcgctctc ctgtattgac gtcataaaag agaacagcgc
     8041 tccagccagc actcctttcc agatcagagg aagagtgatg tccttgaatg tcttgaggcc
     8101 tcctgcgcca acagattcgg ccgcctcctc catagaatgg tgcactacaa gcagagagct
     8161 ataggacgat cgcacagtgt aaggaagtcg ccgcacggcc agtactatgg ggagtatgac
     8221 ccagctgttt gctagtggcg ttgctaagag aatcggagta ttaaaagccc tgatataggc
     8281 gatgcccaat gcagtgcccg gcagagccag caccagcatt gtcaagatat ccagtgctcc
     8341 acgagtgcgc atctgagtcc tcgccatgat ccacgatatt ggcacgccta tcagaatgca
     8401 cattattatc gctagtatgc agaatcggaa tgtgttgatt atatagccag gtgcgtacag
     8461 caagaccttc tcataatgga ccatagtcca tgatgtaggc aaaggcgaca tggaccagct
     8521 tcgcgcaaac gaggctaacg caactcccgc atacggtata aaacagatga gcattagtag
     8581 aattagaaac accagagtca aaacagcccc tttacccttg agcggttttc tttccacggc
     8641 tctgtaggag agagtgctgt aatctttcag cgaaatgtac ttcttggcta tcaacagaaa
     8701 tacaattgaa agaagcacca ttacggcgcc cactgtgatt cccattttaa acagatgcct
     8761 atcggtgaac tgttggatat ccatgtaggc taatggagca atgaagttgg tcagtccaac
     8821 caccatggga gccgcaaaat ctgcgaaagc ccctatgaac acaagaagcg ccccggtaat
     8881 aaagcccggt atggtcaatg gaatagtcac atcaaagaga cgcctaaagc ctacagaccc
     8941 aacgctctct gcagcttcct ctagtgaggc atctattttg ctgagcgagt cgaggacgtt
     9001 cagcgtgatg aacgggaaca agtgacaggt ttccaccaac agaagaccgt gccagccata
     9061 tataaagtta aacggcaagt gattatatag ccagttggac atgaaaggcc atgatttctc
     9121 caggccttcc gcaagctggt ataggagctc gtttacggta cctcctctgc cgagtatgaa
     9181 gatgaagccc aacacaccta ctagaggagg cattactatg ggaacggtag taa
//
LOCUS       DAOMZL010000068         9103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_7221_length_9103_cov_18.909262, whole genome shotgun sequence.
ACCESSION   DAOMZL010000068 DAOMZL010000000
VERSION     DAOMZL010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9103
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7221_length_9103_cov_18.909262"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="PLQ30_06190"
     CDS             <1..1237
                     /locus_tag="PLQ30_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02071.1"
                     /translation="DLVRRTVRMISREAKLPFEVVMLIIAAVVLLVTGVMLFLIYNGI
                     LPYYENGLYGLLLIIFALNTITLGRTPFGDIPRSKLLLVLGLLVAVIGMVTCLIPISI
                     RFPRVLLFFCLGPGGLLLFLGMLLAKDKFRSWVKYGGIFRHLMLACSAVYILSIAIAL
                     LIWKQDLLTTPMTAVVILFFGAAVFYLAIVLGEIDRTYPEAARLPIGDIKLSIEQTMI
                     IFTSVFMIIIGLVLIPVNLGMLPFSSSAQLGILMVIFAIQMLAFGNTPIGPFPRTWLM
                     IGLGILAVVLGAISSIIPGILVSLLTILIGLLNILGGIIALTKILVQGHRQEAVSRDP
                     SPPILRRLFITQLSLNLLQILFGTSMLIPGLVPGMIIGVILAANGAVLLYLVSILSST
                     IDWKDRFPLWFGKTKPRLF"
     gene            1204..1680
                     /locus_tag="PLQ30_06195"
     CDS             1204..1680
                     /locus_tag="PLQ30_06195"
                     /inference="COORDINATES: protein motif:HMM:NF018066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HPW02072.1"
                     /translation="MVWENKTTPILTGNMMGIPKIYADIEDLHILADAYRTRLSFEGS
                     TFLELEMTQLKPLDMEQVNAMNTDINTFGWRYIPKVGEPGADLSQPILFPMHNEAKAA
                     CLGSGKVKWTESTWDQNPMQFYIINALAELPIIEMKPAILMQGREILTEARGRVLK"
     gene            1771..2577
                     /locus_tag="PLQ30_06200"
     CDS             1771..2577
                     /locus_tag="PLQ30_06200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW02073.1"
                     /translation="MKEYYENKVAVVTGGASGIGLALCELLLSFGAKAVVLADINADK
                     LKEESARLENEYPGKVLGIQTDVTKQEKVYEMIQKAAKFGSGQIHFLFNNAGLALFKA
                     FDETTDADWKFAFDINFFGALYGIRAVLPIMRAQGGGHIANTASGAAFSPMAYQSMYT
                     ATKSALVGLTGSLRYEFWDENIRFSTVIPGTVATPIYDKVGGPPESAITPDESARGIL
                     KGLAANERVIIVTEPDRNGAINAFHPETAKYTDEILLDIARQRRRGNIGF"
     gene            2937..3446
                     /locus_tag="PLQ30_06205"
     CDS             2937..3446
                     /locus_tag="PLQ30_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02074.1"
                     /translation="MNKQLLSLIAIGLLSTFACFAQNPLPLYGNSAAKADTQLTIESM
                     LSYAIQDEYVAEAEYEAIMEKFGYMAPYSNIKEAEGQHIAWLKLLFTSYSLKVPENNA
                     SSYIYVPTTLKEAAETGIRAEIDNIAMYERFLAQPILADPRYADIVAVFVRLRDASKN
                     HLEAFRRQA"
     gene            complement(3644..>5119)
                     /locus_tag="PLQ30_06210"
     CDS             complement(3644..>5119)
                     /locus_tag="PLQ30_06210"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HPW02075.1"
                     /translation="PLSGTATISGAALWRLFDQNFHSITSSRSDSDGEVTWSGTPAGA
                     EAKGAEEKPEDMAGTHGATGAWLVVYASSDSGATVCLRQKASTENVSLGKAPQKLSGL
                     VPVATYGEGCSYRSEIGGYTVLSLKGSWHEMGRQYGALAAVELKAFYTAIEKDLAARG
                     LGQEHRDAIEATYQTYTPEMKQLMEGMAETSGLSLAEHKLLDASFYLLPDFVIEAAQA
                     RAACSGIAVSSPRTADGKLYFARNWDMTQDAMRPYLRYMALVAFNPTKSEVPTGSGSG
                     ASGPSLAFANVRPIGQVYVETGINERGIFVELNNGSASDPNTNPEARFSVASLFEFLR
                     TSETFDQMVQNIVNTPMDASYIIQVASAERAVSVEKPTFDARVIEQHDGALYALNNFA
                     RPTYEPWKGKIVELPDNAFDDRQVCLDRMFASQEWQKGVDLAMVKAMMDKTIQQGGPA
                     VEGDLFGTVLQVIVIPEDMRLLFRGYGYSDWADIDLLEIFG"
     assembly_gap    5120..5219
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5220..6816)
                     /locus_tag="PLQ30_06215"
     CDS             complement(<5220..6816)
                     /locus_tag="PLQ30_06215"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPW02076.1"
                     /translation="MMFERTVSGYRTQLRILVVLMLFALVVASAFGVSFSNLQIEAIA
                     QEGSAAVQSIMEKKHATAVTVILADANGIIWQGAYGEMEHGKGIPPTKDTLFGICSVS
                     KTFTAVAVMKLVDRGMVSLDEPVVTYIPDFKMEDPRYRDITVRMLLNHSSGLPGQSIL
                     GGMTLSKPYPDYAEGVIRAQWSQHLKFEPGTNAVYTNDGFSILEVLVKRVSGLRFVDF
                     VQREIFDVLGMNSSRFLVSQLPENSYAVPNPADDFSTDAYLNMYGTGGIFSSAADMAK
                     FATSFLTPGRLLSAESIKEMGEDQTKNLFYPIGRDDGFRYGLGWDSYSQPGMAAVGID
                     AWQKGGDLGFYGATLVVLPKEGLTATVLGASNFSSSDAGAIAERVLLKALVEEGRLKA
                     MPKPSPLSAARAALPDFRERAMIAGYYAGMPYSLTRAVFDDEGRLTIEAKQGESWVPS
                     FAMLEKRVDGWYSNDDSPVSVKFVPHEGKVFYVIRFPSGYEHYVTSAPLAQKLDSGPV
                     LSEAWKNRCDSEWLLASDIVDAED"
     gene            complement(6922..8718)
                     /locus_tag="PLQ30_06220"
     CDS             complement(6922..8718)
                     /locus_tag="PLQ30_06220"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPW02077.1"
                     /translation="MFIKIGRLIAVLCIWGFALSALPAQNILDPSWSGVADEIRPKIQ
                     QRMSESDIKGLMIVLVDEEKTVWSEGFGFSDISTNKKVDADTMFEIGSVSKTFSGLMV
                     MQLYEQGKLDIDKPLVTYIPEFKLMPPVGNFPRVDRPITVRDVLTHHSGIPGDLMNGA
                     FGIKYDKSFLDTLLAWLANDSATYPPGYRWAYSNTAVALLERVIENASGRPFAEYSQD
                     FLDSLGMAPASYFRDNPDLLNRLSRAYIGDVDIGMLQLNVPSSGSIAASANQMGHYLR
                     MLLNEGTLDGRVIVQPETFRAMIQVQNADNALDLTRKMGLSFVLDDADLAWAGPLLWH
                     NGETTAFTSHMEALRNHDIAVFVSVNTKDENNVAGDIAHAALESALAIKRGLARPAQH
                     VSDVSLKSISLPLAFLENFSGSYAGDDLGQVQILRPSNGGLSWDQLEIGSAGQIERNN
                     QGTLTLWSDGFFRTSASADMAFEFREIGGRFLMIGHTGGTQKIFGDRFAAHPLSEAWR
                     ARIGEWVSVDKPLIEAGQRFGAVPQVQLMIVDGILVMERSGQLLVVQPVSDEVGYIRG
                     LGRIGGSALRFQNRDGEQELRFMMDVFRFGAK"
     gene            8887..>9103
                     /locus_tag="PLQ30_06225"
     CDS             8887..>9103
                     /locus_tag="PLQ30_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02078.1"
                     /translation="MKIPSKGVHFKAANDRCNKENLGLPTGPFHAGIDYSYGNLARDT
                     TNNENASQLVVEMVPGLDLILVSHDHQG"
BASE COUNT         2324 a         2260 c         2076 g         2343 t
ORIGIN      
        1 agacctggta agaaggacgg tgcgtatgat ttccagagag gcaaagctcc ctttcgaagt
       61 cgttatgctg attatcgctg cggtagtctt actagttact ggggttatgc tcttcttgat
      121 ctataacgga attcttccct actatgaaaa cggtttatat ggattgcttc taatcatatt
      181 cgccttaaac acgataaccc ttggaagaac cccatttggt gacataccca ggtcaaagct
      241 cctattagtc ttaggattgt tggttgcggt aattggtatg gtaacctgtc ttattcctat
      301 ttcgattcgc tttccccgtg tgctgttgtt tttctgtctt ggtccgggcg gcttgcttct
      361 ctttttgggg atgttgttgg ctaaagacaa atttaggagt tgggtgaaat atggtggaat
      421 ttttcgacat ctgatgcttg catgcagcgc ggtctatatt ttatccatag ccatcgcgct
      481 gcttatttgg aagcaggatc tattgacgac gccaatgacg gctgtggtga ttctattttt
      541 tggcgcagca gtattctatc ttgccatcgt gctcggagag attgatcgga catacccaga
      601 ggcagcgaga cttcctattg gcgacatcaa gctttccatc gagcagacga tgattatatt
      661 cacgagtgtt ttcatgataa tcatcggttt ggtgctgatc ccagtgaatt tgggcatgct
      721 tcctttttca agcagtgcac aattgggaat cctaatggta atctttgcta ttcagatgct
      781 tgcattcggg aatacgccga ttggtccgtt tcctcgtaca tggctcatga ttggacttgg
      841 gattttggcc gtagtgttag gggccatctc aagcataatt cccgggatcc ttgtatcgct
      901 gctgacaatt ctcattggtt tgctaaacat cttaggcgga attattgctc tgactaaaat
      961 cttagtccaa ggtcatagac aagaggccgt atcacgcgat cccagtccgc cgatattaag
     1021 aagactcttc ataacacagc taagcctgaa tctccttcaa atcctattcg gtacatcgat
     1081 gctcatccct ggcttggtac cgggaatgat catcggcgtg attcttgctg cgaatggggc
     1141 tgttcttcta tatcttgtga gtattttgtc cagcacgatc gattggaagg atcgttttcc
     1201 cttgtggttt gggaaaacaa aaccacgcct attctaacag gaaacatgat gggtatcccg
     1261 aagatctacg ctgacattga agacctgcac atccttgcgg atgcataccg tactcgcctg
     1321 agtttcgaag gtagcacctt tttggaactt gagatgaccc agcttaaacc attggatatg
     1381 gagcaggtaa atgccatgaa tactgacata aatacatttg gatggcgcta cattccgaaa
     1441 gtaggcgagc ctggggcaga tttgagtcag cctattcttt ttccaatgca caacgaagcg
     1501 aaagcagcct gcttgggtag tgggaaagtc aaatggaccg aatcaacttg ggaccaaaac
     1561 cccatgcaat tctacataat aaatgctctt gcagaactgc caattatcga aatgaagcct
     1621 gccatattga tgcaaggacg ggagattctc accgaagcgc gaggacgtgt tcttaagtag
     1681 atgcgtattt ccatgccaat ttgagcagcc gatatctaaa gcagatatcc atctctggaa
     1741 tctgcataat tttaaaagga ggattttgga atgaaagaat attatgagaa caaggttgcc
     1801 gtagtaaccg gtggagcttc tggaataggt ttggcactgt gcgaattgct cctatcattt
     1861 ggcgcaaagg ctgtcgttct agcagatata aatgctgata aattgaaaga agaaagcgcg
     1921 aggcttgaga acgaataccc tgggaaagtc ctgggaattc aaaccgatgt taccaagcag
     1981 gaaaaggtct atgagatgat tcaaaaggcg gcgaaatttg gaagcggtca aattcacttc
     2041 ctttttaata atgccggtct tgcgttgttc aaggctttcg acgagacaac agatgccgat
     2101 tggaagtttg cctttgatat caattttttt ggtgctttat atggtatccg agcggtattg
     2161 cccatcatgc gcgcacaagg gggcggacat atagcaaata ccgcctcagg agcggctttt
     2221 tcaccgatgg cctatcagag catgtacacg gccaccaaat ccgccttggt agggcttacc
     2281 ggttcgctgc gctatgagtt ttgggatgag aacattcgtt tttctactgt cattcctggt
     2341 accgttgcaa cgcccatata tgataaagtc ggaggccctc ccgaatccgc aataactcca
     2401 gatgagtccg ctcgtggaat actcaaggga ttagcagcaa acgaaagagt tatcattgtt
     2461 accgagcccg accgcaatgg cgcgattaat gcctttcacc ctgaaaccgc aaagtacaca
     2521 gatgagattc tattggacat cgcacgtcaa agacggcgcg gcaatattgg cttttgagcg
     2581 actttctcag aatgcgaagt cagtaaaaaa aagaggtaga tagatcgcga tcgcgcaata
     2641 gcattgaaca aggcaaaata tatagtcgcg ctcttaagcc tgattcactt tccctcaaca
     2701 ttcttcagct ttataatatc cataatgaca agaatcatga gctttataga atacaaaacc
     2761 atgacccggc caaagcatcc atacgctgat gttctaagcc ctttaatctg gccatatgta
     2821 gcatcaccta tctgctccaa attgcgaact aaatcttgca ctatccgcgg cggtttttgc
     2881 aaccacaatc gcactttagg cgactataaa gtagacaaac cgcaaggaga tcaaagatga
     2941 ataagcaact gctttcatta attgctattg gacttcttag tactttcgct tgttttgcac
     3001 agaaccctct accgctgtac ggcaattcag ccgcaaaagc tgacacgcag cttactatag
     3061 aatccatgct ttcctatgct attcaagatg aatacgtggc tgaagcagaa tatgaagcta
     3121 tcatggaaaa atttggttat atggctccgt actccaatat taaagaagca gagggacaac
     3181 atatcgcatg gctcaagctg cttttcactt cctattccct aaaagtcccg gagaacaacg
     3241 ccagcagtta catttacgtt cccacgacgc tgaaagaggc tgcggaaact ggaattcgtg
     3301 ctgagataga taacattgcg atgtatgaga gatttcttgc tcagcccatt cttgccgatc
     3361 cgcgctatgc tgacattgtt gcagtgtttg ttagattgag agatgcttca aaaaatcatc
     3421 ttgaagcatt cagaaggcaa gcataagcaa caaacctacg gcgagtactt gggcttgagg
     3481 gaataaatcc ccttgagctg tatagggcga tggaaggctg agccctaagc aagagcggat
     3541 cgaaatgaag ctggcgaagg gatcgcgccc gagcgcatgc aagcgttcga gcgcaagatc
     3601 gcctctcggc attcatcgct ttgctatatt gcttttcaaa tcatcatcca aagatttcaa
     3661 gtaaatcgat gtctgcccag tccgaatagc catagccacg gaaaagcagt ctcatgtctt
     3721 caggtattac aatgacttgc agcactgttc caaagagatc gccttccacc gccggtccgc
     3781 cttgctgaat ggttttgtcc atcatcgctt tgaccatagc cagatctact cccttttgcc
     3841 attcttgcga tgcgaacatt ctatcaaggc aaacttgcct gtcgtcgaaa gcattgtccg
     3901 gcagctcgac gatctttccc ttccatggtt cgtaggttgg tcgagcaaaa ttattgagcg
     3961 catagagcgc gccatcatgc tgctcgatca ctcgagcgtc aaaagtgggc ttttccaccg
     4021 aaactgctcg ctccgcactt gccacttgga tgatatacga ggcatccatc ggcgtgttca
     4081 cgatattttg caccatctgg tcgaaggttt cgctcgtcct taagaattca aagagcgatg
     4141 ccactgagaa gcgcgcttcg gggtttgtgt tcgggtcaga agccgaaccg ttgttgagtt
     4201 cgacaaaaat accgcgctcg ttgatacccg tttccacata cacttggcct atcggcctta
     4261 cgttcgcaaa cgcgagcgaa ggaccagatg cgccgctccc cgatcctgta ggtacttctg
     4321 acttcgtcgg attaaaggca acgagtgcca tgtaacggag ataaggccgc attgcatcct
     4381 gagtcatgtc ccagttgcgg gcaaagtaga gtttgccatc ggcggttctg ggactggaaa
     4441 ccgcaatgcc cgaacaggcc gcgcgggctt gtgccgcttc aatgacaaaa tcgggtagca
     4501 aatagaatga tgcgtcgagc aatttgtgct ccgccaatga caagcccgag gtttctgcca
     4561 taccctccat gagctgtttc atttcggggg tataggtttg gtatgtcgcc tcgattgcgt
     4621 ctcgatgctc ttggccaagt ccacgcgccg caaggtcctt ttcgattgcg gtgtaaaacg
     4681 ctttgagctc tactgccgcg agggcgccgt attgcctgcc catctcgtgc cagcttccct
     4741 ttaggctgag caccgtgtaa cctccgatct cgctgcgata gctgcatccc tctccatagg
     4801 tggccactgg tacaaggccc gacagttttt gaggcgcctt gcctaaagag acattttccg
     4861 tgctggcctt ttgtctcaag cagactgttg cgcctgaatc agacgaggca tacaccacca
     4921 accatgcccc cgtcgcgcca tgagtcccag ccatgtcttc cggtttttct tcggcgcctt
     4981 ttgcttcggc gcctgcgggc gtgcccgacc atgtcacttc tccgtccgaa tcgctacggc
     5041 tggacgtgat actgtgaaaa ttctggtcaa agagcctcca aagcgccgcg ccagaaattg
     5101 ttgctgtccc cgataatggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     5221 atcttcggca tcaacaatat cgctagccag aagccactct gagtcgcatc gatttttcca
     5281 ggcttctgac agaaccggac cggaatccaa cttttgggca agtggcgcgg aggtcacata
     5341 atgctcgtat cctgatggga agcggattac atagaatacc ttgccttcgt gaggcacgaa
     5401 ttttacagac actggcgaat catcatttga ataccaccca tctacgcgct tttcaagcat
     5461 tgcgaacgaa gggacccacg attcgccttg ctttgcttcg atggtcaagc gtccttcatc
     5521 gtcgaacacc gcgcgggtaa gcgaataggg cattccagcg tagtatcccg cgatcatcgc
     5581 gcgctctcta aaatcaggca gtgctgccct tgcggcgctc aaaggcgatg gcttgggcat
     5641 tgctttgagc cttccttctt ctacaagagc cttaagaagc actctttccg caatagctcc
     5701 tgcatctgaa gacgagaaat tgctcgcgcc aaggaccgtg gcagtaaggc cttccttagg
     5761 aagcaccaca agcgttgccc cataaaaacc caagtctccg cctttctgcc atgcatcgat
     5821 gcctaccgca gccatgcctg gctggctgta cgagtcccac ccaaggccat agcgaaatcc
     5881 gtcatcccgc ccaataggat aaaacagatt ctttgtctga tcctctccca tttccttgat
     5941 agattctgcg gaaagcaggc gtcccggggt taaaaaagat gtggcaaatt tggccatgtc
     6001 agcggcagaa gagaaaatac ctccggtgcc atacatattg agatacgcat cagtagaaaa
     6061 atcatccgcc ggattcggaa ctgcgtaaga attctctgga agctgcgaaa caagaaagcg
     6121 cgatgaattc ataccgagga catcaaaaat ttcgcgttgc acaaaatcga caaatctcag
     6181 cccgcttacg cgcttgacga gcacttcaag aatcgaaaaa ccatcatttg tgtagacagc
     6241 attggtcccc ggttcaaatt tgaggtgctg cgaccattgg gcgcgaatca cgccttcagc
     6301 ataatcggga tagggtttgg agagcgtcat tcccccgaga atcgattgcc cagggaggcc
     6361 tgagctatga ttgagcaaca tgcggacggt gatatcgcgg taacgaggat cctccatttt
     6421 gaaatctgga atgtaggtca ctacaggctc gtccaaagac accattccac gatccacaag
     6481 tttcatcaca gccacagccg taaaggtctt tgagactgag cagattccga aaagcgtgtc
     6541 ttttgtcggc ggaatacctt taccgtgttc catttctccg tatgcccctt gccagataat
     6601 gccgttcgca tctgcaagta tcacggtaac agcagttgca tgtttttttt ccattatcga
     6661 ttgaaccgcc gcagatccct cctgcgcgat ggcttcgatt tgtagattac tgaacgaaac
     6721 accaaacgca gatgccacga ctagcgcgaa tagcataagg acaaccaaaa tcctcagctg
     6781 cgtacgatat ccagagactg ttctttcaaa catcatgttc ttctcctcgc tgagcttatt
     6841 cataaactca ccgtgtcgct tcttagcttt ttatcctctt ttacgaagct ttatcacaag
     6901 catctactca ccacatctat tttatttagc accaaaacga aacacatcca tcatgaaacg
     6961 tagttcttgt tccccatcgc ggttttgaaa gcgaagtgcg ctccccccaa tccgccccaa
     7021 tccacgtata taaccgacct catcgcttac aggttgaaca acgagaagct gtcctgaacg
     7081 ttccatcacc aggatgccgt ctacaatcat gagctgcacc tggggtactg cgccaaagcg
     7141 ctggcctgct tcgataaggg gtttgtcaac agaaacccat tctccgatac gggctctcca
     7201 cgcttcggag agaggatgcg ccgcaaaacg atcgccaaat atcttctgag tgcctcccgt
     7261 atgacctatc attaagaatc tgccgccgat ttctctaaac tcaaaagcca tatctgcact
     7321 tgccgatgtt cgaaagaacc catccgacca gagtgttagt gtgccttgat tattccgctc
     7381 aatttggcct gctgatccaa tttcgagctg gtcccaagaa aggcccccgt tcgatggtcg
     7441 caaaatctga acctgaccta aatcgtctcc cgcgtaactg ccagagaaat tttccagaaa
     7501 cgcaagcgga agtgaaattg acttgagtga aacgtctgag acgtgctgcg caggccgagc
     7561 gagcccacgc tttatggcga gcgcactctc taacgccgca tgtgcgatgt ctccagcaac
     7621 attattttcg tcctttgtat tgacactaac gaaaacagca atgtcgtggt tcctcaacgc
     7681 ttccatgtgc gatgtgaatg ctgtcgtttc cccattgtgc catagcagcg gacccgccca
     7741 tgcaaggtca gcatcatcga gcacgaaaga aagccccatt ttacgcgtca aatcgagtgc
     7801 attatcggca ttttggactt gtatcatcgc cctaaaggtc tctggttgaa caataactct
     7861 gccgtccagc gtcccttcgt ttagcagcat tcgaaggtag tgccccattt ggtttgcact
     7921 tgctgctata gagccagagg aaggaacatt gagctgcaac atcccaatat caacgtctcc
     7981 aatataggct cttgaaagtc gatttagaag gtcgggatta tctctgaaat aggatgcagg
     8041 agccatcccc aaggaatcaa gaaaatcctg cgagtattcc gcaaacggcc tgccagaggc
     8101 gttttctata acgcgctcca ggagtgccac ggcggtgttc gaatatgccc atcgataccc
     8161 tggcggatag gtcgccgagt catttgcaag ccatgcgagc aaggtgtcga ggaagctctt
     8221 atcgtatttg ataccgaaag cgccattcat taaatcacca ggaattcctg agtggtgcgt
     8281 gagtacgtct cgcaccgtta tcggcctatc gacgcgagga aaattgccca caggcggcat
     8341 gagcttgaat tccggaatat aggtgaccag cggcttatca atgtcgagtt tgccttgttc
     8401 atagagttgc ataaccatga gcccactgaa agtcttggaa acagaaccaa tttcgaacat
     8461 cgtatccgcg tctactttct tgttcgtgct gatatcggaa aatccaaaac cttcgctcca
     8521 cacagttttc tcttcatcca caagcacgat catgagccct tttatatcag attcgctcat
     8581 cctctgttgg atttttgggc gaatttcatc tgctacacca ctccacgagg gatccaatat
     8641 attttgcgca ggcagcgcgg agagcgcaaa accccatatg cataggactg cgatcaaacg
     8701 ccctatctta ataaacatat acctccccct cagagaacag gccactaatc ttctttttga
     8761 tctgatgaaa atgagcgaga atttacaaca tttttaagaa aagtcattat aagtgtaaat
     8821 cacaatggtc attgagtaaa gctgaaaaag gagatccgct ataggatcac tgcaaatcaa
     8881 aaccatgtga aaatcccatc taaaggcgtg catttcaaag ctgcaaacga taggtgcaat
     8941 aaagagaatc ttggcctacc caccggccct ttccatgctg gtatcgatta ttcctatggt
     9001 aatttggcgc gcgatactac aaacaatgaa aatgcatcac aactcgttgt ggagatggta
     9061 ccaggcctag atctcatcct tgtaagccac gatcatcaag gct
//
LOCUS       DAOMZL010000069         8937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_7377_length_8937_cov_13.423666, whole genome shotgun sequence.
ACCESSION   DAOMZL010000069 DAOMZL010000000
VERSION     DAOMZL010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 8937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8937
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7377_length_8937_cov_13.423666"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLQ30_06230"
     CDS             complement(<1..340)
                     /locus_tag="PLQ30_06230"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPW02079.1"
                     /translation="MISYKELGLVNSVELFRKAMEGGYAIPAYNFNNMEQMQAIIQAC
                     VETKSPVILQVSSGARKYANATLLRSMAKGAVEYAKELGYPIPIVLHLDHGDSFELCK
                     DCIENGFSSVM"
     gene            543..1433
                     /locus_tag="PLQ30_06235"
     CDS             543..1433
                     /locus_tag="PLQ30_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02080.1"
                     /translation="MAQAVFDEAFMLLYPDLSQAVASKFQLDSVASPGAATQLLEKMT
                     DPKTTDNQSLKIIIASPSVTPVIQEFASKGCFALINLLTPANGFYSVEWDNAWAYRQI
                     GLLAGYRVAVLQKETDKNASAALLFARGIGRSDTELEVFNRAFRESYLRATMGTEQEN
                     SSNQAAIMPENALKVFDVDNLNLPGDRLEQVLSVVEQIEEIKPSVLIFACTSRLALEK
                     TCEIQGIDIIADIRGLGKDIPHKKLFASLGENRSNLLRALLKIVKEIEDGNTEPHSVY
                     LRPSLALSKEARRIQQELKP"
     gene            complement(1457..4042)
                     /gene="clpB"
                     /locus_tag="PLQ30_06240"
     CDS             complement(1457..4042)
                     /gene="clpB"
                     /locus_tag="PLQ30_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPW02081.1"
                     /translation="MNYEQFTLKAQEALRNAASIAQKNDHSSIEPEHLLLALLDQEGG
                     VVSSIIERLGVDRTKLEQRLADLLKTLPKAYGETAQVYFSPAMQKVIAKAESESESMK
                     DEFVASEHLFLALLITESRAASLLRSIGVTHEGVLAALKSIRGSQTASDENAEEKYQV
                     LEKYTRDLTALARAGKLDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIISGDVPDSLKDKKVLALDLGAIVAGSKFRGEFEERLKAVITEITKSEGKIILF
                     IDELHTLVGAGAAEGAMDASNLLKPALARGELRAIGATTLDEYRKHIEKDAALERRFQ
                     PIYCAPPSVEDTIAILRGLKERYEVHHGVRIRDDALIAAAVLSDRYITSRFLPDKAID
                     LVDEAASRLKMEIESQPTELDQIQRRLIQLRMEADALKKETDPASIERRSKLEKEIGE
                     LSEKRDEMMARWQQEKQLIEDIRQLKQQIEEYKIQVDQLVREGQLAKASEIKYGKLVE
                     AQQKLEELSKKVQEEEDKPRLLREEVSDEDIAKVVSLWTGIPVSKMLSSEIQKYVELE
                     SQLGKRVIGQEKALRAVADAIRRNRAGLSDPRRPMGSFLFVGPTGVGKTELAKALAEF
                     LFNDERALTRIDMSEYMEKHSVSRLIGAPPGYVGYEEGGQLTEAVRRRPYSVVLFDEI
                     EKAHPEVFNVLLQLLDDGRLTDGQGRVVDFRNAIIIMTSNIGSEFILTMKSMDEIRDR
                     IDGLLKTYFKPEFLNRIDEIVVFDRLDESMIRNIAKLRLSELADRLAQRNIKIEIQDK
                     AIDYIAKEGYDEQFGARPLKRAIQNLLENPIAKKVLSGEIMEGDTIHVSLSGSELAIS
                     TEHAA"
     gene            complement(4192..4668)
                     /locus_tag="PLQ30_06245"
     CDS             complement(4192..4668)
                     /locus_tag="PLQ30_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HPW02082.1"
                     /translation="MKERAERLKVIKAIIKENRVDSQEKLLQILEDHGYSVTQATLSR
                     DLKYLKVGKLSDGHSGYYYSIPSEEERRESERHYIQDFLRGYVSLDWNQSLVVVRTFS
                     GHSDSVALAIDNMGFDEVIGTISGRDNVVFIALKKGVSGDDFIRSLKAKIPEFEEE"
     gene            4805..5815
                     /locus_tag="PLQ30_06250"
     CDS             4805..5815
                     /locus_tag="PLQ30_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPW02083.1"
                     /translation="MQNSRQIISLPYRSTAFWLSLAHEPVESCKRNELGVPLDPILAQ
                     AVPSTAESRASPWDRCDPLGEESHSIANRVIRHYRSRVLVRATGQCFLFCRHCYRRSL
                     LPSELEFIDESTIDKLILLLRSHPEIREVLISGGDPLIASDEKLEGLLSAIRSVERPI
                     LIRLCTRAPIVLPKRISDNLISLLSELRPLQIMLHINHPRELSPEFLDKSESILKAGI
                     PLHSQTVLLRGINDNAETLIELFSILNLHSIHPYYLFQGDLAIGTAHFRVPLSQGLII
                     YEKLKRELSGLELPRYSVDAPGGGGKVYLPEGIVERKNDSWVLRLPDGSLCEYPEEHL
                     QC"
     assembly_gap    5837..5936
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5937..7501)
                     /gene="guaA"
                     /locus_tag="PLQ30_06255"
     CDS             complement(<5937..7501)
                     /gene="guaA"
                     /locus_tag="PLQ30_06255"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPW02084.1"
                     /translation="MDKIIILDFGSQYTQLIGRRIRDLGVYSEILPGEKALESAASKG
                     CLGFILSGSPFSAMQKEAPALHPSILESGLPILAICYGIQWLTLQSGGIVERSKAHEY
                     GPMTVSFLPKARQGGNPWSLFSEGLPERFLSWMSHGDSIVLPGDGWEIIGVSESGVPA
                     ILAHKSKPWFGLQFHPEVSHCEHGMDILSRFVFGVCKAKATWSLETYLDEESKTIETR
                     VKSEPVLLLISGGVDSTVTGAFLLRCLNPEQVHLLYIDTGLMRKAENEEVMLNLSRLG
                     ARHLETVDAESRFLEALKGVLDPEQKRRIIGDMFIRVQEEAVQRLGIPNAFLAQGTLY
                     TDLIESGKGIGDKAQVIKSHHNVRSPLIEKKRAEGRVIEPLDKLYKDEVRALGRALGL
                     PEEIVGRHPFPGPGLGVRILGEVTKEKCDILREADSIYIEELRKRGLHEQIWQAFCVL
                     LPVRSVGVMGDERVYRYVVALRAVRSEDGMTAQPFAFEMKDLFEISACITNQVPQIGR
                     VVYDISSKPPATI"
     gene            complement(7489..8715)
                     /locus_tag="PLQ30_06260"
     CDS             complement(7489..8715)
                     /locus_tag="PLQ30_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HPW02085.1"
                     /translation="MNVVIIGAQWGDEGKGKIVDFLAADAQIVVRFSGGANAGHTIVL
                     GGERFALHLVPSGILYPDKTVILGSGMVIDPEALFGELRDLENKGIDWKGRVFISDRA
                     HLVLPGYREMDREADARRAKPIGTTGKGIGIAYALKASRDGIRIADIDNIERLVNLGS
                     EDREWLFQWKDRLLPLKIDLAAYLRSHRNTWRLFEGAQGALLDIDTGTYPFVSSGMSC
                     AAGAAAQGGIGPRAIDRVLGVFKAYSTRVGNGPFPTEFADDSPGSLSQYIRKVGNEYG
                     TTTGRPRRCGYLDLVALRYACAVNSIDRLVLTHLDVYDALEEFEACIGYHIDGRLTED
                     FPASIGDLEVAQPVLRTFKGWKRSIGNTRTYGDLPLNAREYIEFIEEFTETPISIISV
                     GSDRNQTIVRESPWIR"
     gene            complement(8732..>8937)
                     /locus_tag="PLQ30_06265"
     CDS             complement(8732..>8937)
                     /locus_tag="PLQ30_06265"
                     /inference="COORDINATES: protein motif:HMM:NF020772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HPW02086.1"
                     /translation="SSDWALLLRSGKSADLAAKQIRESIYNFNHIYEMLSAHTVETEW
                     LTSLEKRNNIFPYMNYHVFAPKK"
BASE COUNT         2257 a         2239 c         1912 g         2429 t
ORIGIN      
        1 tcattacgga agaaaagccg ttttctatgc agtccttgca taactcgaaa ctatctccat
       61 gatctaagtg caatacaatc ggaataggat agcctagctc ttttgcatat tcgacggcgc
      121 cttttgccat cgatcgtaat agcgtggcat ttgcatattt ccgtgcacca gaggatacct
      181 gaagtatgac aggacttttc gtctcaacgc aagcttgaat gatggcttgc atctgctcca
      241 tgttgttaaa attataagcc gggatagcgt atccaccctc catagccttg cggaaaagtt
      301 caacactgtt taccaggcca agctctttat aactgatcat ataatcctcc gaaaaactat
      361 aaattctctt tgctgcaggt tattgtcgtt atatagagaa ttatgcgatt atgcaacagg
      421 cttatcaaat gtggtacaaa aataagcact tctgcatttc cttccgtcag ggactcgctc
      481 gcaaaatgct gtatactacc attgtcctga tcagcctcct attctcaagc tgcagacatc
      541 cgatggcaca ggcggtcttt gatgaagcct ttatgcttct ctatccagat ctctcccaag
      601 ccgttgcaag taaatttcag ctggatagtg tggcaagccc cggggccgct acacagcttt
      661 tggaaaaaat gactgatccg aaaacaacgg ataatcagtc tttgaaaatc atcatagcca
      721 gtccatctgt aactccagta attcaagaat tcgcctccaa aggctgcttt gcgttaataa
      781 accttctaac tccagccaat ggcttttatt ctgtcgaatg ggataatgcg tgggcatacc
      841 gacagattgg gctcttagcg ggctatcgtg ttgctgttct ccaaaaagag acagacaaaa
      901 atgcctcagc ggccctgctc tttgctcgcg gtataggaag gagcgataca gagctggaag
      961 tattcaatcg agcctttaga gaaagttatc tacgagctac catgggcact gagcaagaaa
     1021 attcttcaaa tcaagccgca attatgcccg aaaatgctct taaagtattc gatgtagaca
     1081 atctgaatct tcctggagat aggctggagc aagtgctctc tgtggtagaa caaatagaag
     1141 aaataaaacc cagcgttttg atctttgctt gcactagcag gcttgcgctg gaaaaaactt
     1201 gtgagattca aggaatagat atcattgccg atataagagg cctggggaaa gatattccgc
     1261 ataagaaatt atttgcatcg cttggagaga atagatccaa tctcctgaga gcgctcctaa
     1321 agatagtgaa agaaatagaa gatggaaaca ccgagcctca ttccgtatat ttaagaccta
     1381 gtctcgcatt atcgaaagag gctcggcgca tccagcaaga gttaaaacct taggcactct
     1441 gcgataaggt gctttctcat gcggcatgct cggtgctgat ggcaagctcg ctaccggaaa
     1501 ggctaacatg aatggtatcg ccttccatga tttccccgga aaggactttc ttggcaatgg
     1561 ggttttcaag caagttttgt attgctcttt taagaggacg cgcgccaaat tgttcgtcgt
     1621 atccttcctt tgcgatataa tcaatggcct tgtcttgtat ctcgatcttg atatttctct
     1681 gcgcaagccg atctgcgagt tcactgagcc tcaatttagc aatattgcgg atcatggatt
     1741 catcgaggcg atcgaaaaca acgatctcat cgatgcgatt taggaattcg ggcttgaagt
     1801 atgtcttaag aagtccatcg atccgatctc gaatctcatc catgcttttc atggtgagaa
     1861 tgaactccga gcctatgttg cttgtcatga tgataatagc attacgaaaa tctacgaccc
     1921 ttccctgtcc atctgttagc cgtccatcgt ccagaagctg tagaagaaca ttgaatactt
     1981 cggggtgggc cttttcgatc tcatcgaata gtactacaga ataggggcgc cttctcaccg
     2041 cctcggtgag ttgaccacct tcctcatatc caacatatcc gggaggagcc ccgatgagcc
     2101 tcgacactga gtgcttttcc atgtattcac tcatatcgat ccgcgtaaga gctcgttcat
     2161 cattgaagag aaattcagcg agcgcctttg caagctctgt ctttccaact cccgtcggtc
     2221 caacaaagag gaaagagccc ataggtcggc gcggatcaga aagccccgct ctgttacggc
     2281 gtattgcatc ggctacggct cgcagcgcct tttcttgacc gataaccctc tttcctaact
     2341 ggctctctaa ctcgacatat ttttgaattt cgctcgaaag catcttggaa acaggaatgc
     2401 ccgtccagag agacacgact ttggctatat cttcgtcgga tacttcttcg cgcagaagtc
     2461 ttggcttgtc ctcttcctct tggacttttt tcgataattc ttcgagcttt tgctgcgctt
     2521 caactagttt gccatatttg atctcagaag ccttggcaag ttgaccttcg cgcacaagct
     2581 gatctacttg gattttatat tcctcgatct gttgcttaag ttgacgaata tcctcaatca
     2641 attgcttttc ttgctgccaa cgagccatca tttcatcgcg tttttcggag agctcgccta
     2701 tctctttttc gagcttgctc cgtctctcta ttgaagcagg gtctgtctct tttttaagag
     2761 catctgcttc catccgaagt tgaatcaacc tgcgctgaat ctgatctagt tctgtaggct
     2821 ggctctcgat ctccatcttg agtcttgagg ctgcttcgtc gactagatct atagccttat
     2881 caggcaggaa tcgagaggtg atatagcgat cggataaaac tgcggcggct atgagcgcat
     2941 catcgcgaat tcgcactcca tggtgcacct cataacgctc cttaaggcct cgcaatatag
     3001 ctattgtgtc ctccacacta gggggtgcac aataaatagg ctgaaatcta cgctcaagcg
     3061 cagcatcctt ttcgatatgc ttgcgatact catcgagagt agtcgcacct atggcgcgaa
     3121 gctccccacg cgcaagggct ggctttaaga ggtttgacgc atccatagct ccttccgcgg
     3181 ctccggctcc aaccagagta tgaagctcat ctatgaagag tattatcttg ccttcagatt
     3241 ttgtgatctc ggtgatgacg gccttgagcc gttcctcgaa ttcgccccga aatttgcttc
     3301 cggccactat ggctcctaaa tcgagggcga gaaccttttt gtcctttaga ctgtccggga
     3361 catcgcccga tattatacgc tgcgctaagc cttctacaat ggctgttttc cctactccgg
     3421 gctctccaat aagcacgggg ttgttctttg ttcttctgga aaggacctgc attaccctgc
     3481 gaatttcttc atctctaccg attaccggat caagcttgcc agcacgagcg agggcggtga
     3541 gatcgcgtgt gtatttttct aagacctgat atttctcctc agcattctca tcggaagcgg
     3601 tctgagaacc ccttatcgac tttaaggctg caagcacccc ttcgtgcgtg actcctatcg
     3661 atctaagcaa agaggctgct ctggattccg ttattaaaag agctaagaaa aggtgctcac
     3721 tggctacgaa ttcatccttc attgattcgg attcggattc ggctttggca atgaccttct
     3781 gcatggcagg agaaaagtat acctgagccg tttcgccata tgcctttggc agcgttttaa
     3841 gcagatcagc gagcctctgc tcaagcttgg tcctatctac tcctaagcgc tctatgatcg
     3901 aagatacgac tcctccctcc tgatctaata aggccaggag cagatgttca ggttctatag
     3961 agctgtgatc attcttctga gctatggaag ctgcgttgcg gagcgcttcc tgcgctttta
     4021 aggtgaattg ttcataattc atagtgtatt tccttcgtga ttcaaaagag attcttctct
     4081 attaagtata caaattcaga caggcttttg gcaaattaaa ttataaaaat tgctctccca
     4141 tgctcagttg actatgaatc accgaaggat tgtgcaatat ataagcgccg attattcttc
     4201 ctcgaattcg ggaatctttg ccttgagtga gcggataaaa tcatcgccgc tcacgccctt
     4261 cttgagcgcg atgaatacaa cattatcgcg tcccgatatc gtgcctatca cttcatcaaa
     4321 gcccatatta tcgattgcaa gggcaaccga atccgaatga cctgaaaagg tacgcacaac
     4381 cacgaggctc tgattccaat cgagcgaaac atagcctctc aaaaaatcct ggatatagtg
     4441 acgctctgat tctctgcgtt cttcttctga aggaatggaa tagtaatagc cagaatgccc
     4501 atcggagagc ttcccgacct ttaaatactt caaatcgcga gaaagtgtcg cttgcgtcac
     4561 cgaatagcca tgatcttcca aaatctgaag tagcttttcc tgcgaatcga ctctgttctc
     4621 tttgatgatg gcttttatta ccttcaatct ctctgctcgt tctttcataa tctctccgat
     4681 aattctacta ttgccacctt ataattcatt attgcatatg taggccaaaa aggcaagaaa
     4741 aagccatatt ctataccctc agcctcattg ctcattatct ttttagaaat tatattgacc
     4801 agagatgcag aattctagac agataatcag cctgccctac cgctcgacgg ctttctggct
     4861 cagccttgct catgagccgg tggaaagctg caaaagaaat gaacttggag tacccctaga
     4921 tcccattctt gcacaagccg tgccatccac cgccgaatcg cgtgcttctc cctgggatag
     4981 atgcgaccca cttggagaag aaagtcattc gattgccaac cgtgttataa ggcattatcg
     5041 ctcccgcgta cttgtgcggg cgaccggtca gtgctttctc ttctgtcggc attgctatag
     5101 gcgttcgctt ttgccctcgg aactagaatt catagatgag tctacgatcg ataagctgat
     5161 attgctcctt cggtcacatc cggagatccg cgaagtactg atttcgggtg gagatccgct
     5221 aattgcatcc gatgagaagc ttgaggggct tctatccgcg atccgctcgg tcgaaagacc
     5281 aatattgatt aggctatgca caagggcgcc catcgtgctg cctaagagaa taagcgataa
     5341 cctcatttcc ctgttgtcag agctgcgccc tcttcaaatt atgctccaca tcaatcaccc
     5401 gcgagaattg agccctgaat tcttggataa atcggaatct atcctcaaag ccggtatacc
     5461 gcttcatagt cagacggtcc ttttgcgggg aatcaatgac aatgcagaga ctttgatcga
     5521 gcttttctct attcttaacc tacatagcat tcatccctac tatctctttc aaggagatct
     5581 agccatcggt accgcacatt tccgtgtacc gctttctcaa gggttaatca tctacgaaaa
     5641 gctgaagaga gaattatcag gtttggaact gccgcgttac tcggtggatg caccgggtgg
     5701 aggaggaaaa gtatacctcc ctgaaggaat agtcgagcga aagaatgaca gctgggtgct
     5761 tcgcttacct gatggaagcc tatgcgaata cccagaagag catttacagt gttgagaagc
     5821 tcaaatctga gggttannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcga
     5941 tagttgcagg cggtttggag gatatgtcgt agacaactct gccaatctga ggtacctgat
     6001 tcgtgataca tgcggatatc tcgaaaagat ctttcatttc gaaggcaaat ggttgagcag
     6061 tcatgccatc ctcgctcctg acagcccgaa gtgcaactac atacctataa accctctcat
     6121 ctcccattac acctaccgaa cgaacaggaa gaagcacgca aaaagcttgc catatctgct
     6181 catgcagccc tcgctttcta agttcttcga tatagatact gtccgcttct cgcaggatgt
     6241 cacatttctc ttttgttact tcacccagaa tcctcacacc aagccccgga ccaggaaaag
     6301 gatgtcgacc tacaatttct tcaggcaagc ctaatgccct tccaagagct cgaacttcat
     6361 ccttgtatag cttatccaaa ggctcgataa ctcgtccttc tgctctcttc ttttcgataa
     6421 gagggctgcg cacattatga tgactcttta ttacctgagc cttgtcgccg atccctttgc
     6481 ctgattcgat caaatctgta tagagcgtcc cctgggcgag aaatgcattg ggaattccaa
     6541 ggcgctgaac agcttcttcc tgaactcgta tgaacatatc gccaattatg cgcctctttt
     6601 gttcagggtc taaaacaccc ttgagcgcct cgagaaatcg agattccgca tcgacagttt
     6661 ccagatgtcg agctccaagg cggctcagat ttaacatgac ctcttcattc tccgcttttc
     6721 gcatgagacc tgtgtctata tataagaggt ggacctgttc aggattcaag catctcagca
     6781 aaaaagctcc agtaaccgtc gaatcgacgc cccctgaaat caagagcaaa actggttcag
     6841 attttactct tgtttcgata gtctttgatt cctcatcgag ataggtttct aatgaccaag
     6901 tggctttagc tttgcagaca ccgaacacaa aacgagacaa aatgtccatt ccatgttcgc
     6961 aatggctgac ctcgggatga aattgcaatc caaaccatgg cttggattta tgagctagaa
     7021 tagcaggaac tccgctttcc gatacaccga tgatttccca gccatctcca gggagcacaa
     7081 tgctgtctcc atggctcatc cagctcaaga atcgctctgg tagcccttcc gagaaaaggg
     7141 accaaggatt cccaccctgt cgggctttag gcaaaaagct cacggtcata gggccatatt
     7201 catgcgcctt ggagcgctct actatgcctc ccgattgtaa agtaagccat tgaataccat
     7261 agcaaattgc gagtatcgga aggcctgatt ccagaatact tgggtgaaga gcaggagctt
     7321 ctttttgcat tgcgctgaaa ggtgagccag aaaggataaa acctagacaa cccttgcttg
     7381 cagccgattc cagtgctttc tccccaggca agatctctga ataaacacct aggtccctga
     7441 tacggcgacc aattagctgg gtatactggc taccaaaatc gagaattatt atcttatcca
     7501 tggactttcc cgaacaattg tctgatttct atccgaacct acagaaatta tcgaaatcgg
     7561 agtctcggtg aattcttcta tgaattcaat gtattcacga gcgttcaatg gcaaatcccc
     7621 ataggtcctg gtgttaccaa tagatcgctt ccatcctttg aatgttctca ggacgggttg
     7681 ggcgacctca agatctccta ttgaagccgg aaagtcctcc gtaagtctgc catcaatatg
     7741 atatcctatg catgcctcga attcttccag agcgtcatag acatccaggt gagtgagcac
     7801 aagcctgtcg atagagttta cggcacatgc gtagcgaagg gctacaaggt ctaggtaacc
     7861 acagcgccgc gggcgccccg tcgtagtacc atactcgttc cccaccttac gaatatactg
     7921 ggacagcgag cctggcgaat cgtccgcgaa ctcggttggg aaaggaccat ttccaaccct
     7981 cgtggaataa gccttgaaga cccccaatac tcgatcgatt gctcgcggac cgattccccc
     8041 ttgcgcagca gcgcccgcgg cgcatgacat acctgaagag acaaatgggt aggttcctgt
     8101 atctatgtcg agcaatgcgc cttgagcccc ctcgaaaagt cgccaggtat tgcggtgaga
     8161 acgcagatag gcagccaaat ctatctttaa aggcaaaagt ctatccttcc attgaaagag
     8221 ccattcccta tcttctgaac ccagattgac gagtctttca atgttgtcga tatcagcgat
     8281 ccggatgcca tctctgcttg ccttaagagc gtaagcaatt ccaatgcctt ttcctgtggt
     8341 ccctatgggt tttgcacgtc gagcatcggc ctctctatcc atttctctat atccgggcaa
     8401 aaccagatga gctctgtcgg aaataaatac cctgcccttc cagtcgatgc ccttattctc
     8461 cagatctctt agctctccga aaagcgcttc cggatctatg accatgccag aaccgaggat
     8521 gaccgtttta tcggggtata gaatgcctga gggtactaga tgtagcgcaa accgctcacc
     8581 gcccagaacg atagtatggc cagcgttagc gccaccagaa aagcgcacca ctatctgtgc
     8641 atctgctgct aaaaaatcga caatcttgcc ttttccttca tccccccact gtgctcctat
     8701 gatgacaaca ttcacgatag actccttatt gctatttctt aggtgcaaaa acgtggtaat
     8761 tcatgtatgg aaatatatta ttgcgctttt ctagactcgt gagccattcc gtttccacgg
     8821 tatgcgctga gagcatttca tagatgtggt tgaaattata aatcgactcg cgaatttgct
     8881 tcgcggccaa atctgctgat ttgccacttc tcaataagag agcccaatcc gaagaca
//
LOCUS       DAOMZL010000070         8614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_7731_length_8614_cov_21.248043, whole genome shotgun sequence.
ACCESSION   DAOMZL010000070 DAOMZL010000000
VERSION     DAOMZL010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 8614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8614
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7731_length_8614_cov_21.248043"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PLQ30_06270"
     CDS             <1..516
                     /locus_tag="PLQ30_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPW02087.1"
                     /translation="GFDTVPQANLSGSSQLATIILMFIGASPGSTGGGIKTVTLYILL
                     LVALRMREGGDTIVDRYRTVTPNTVFKAITALVRGATIVLVATTILLVSERAAGHTVG
                     LETAIFECVSAFGTVGLSIGITPYLTAVSKLVLIAAMFMGRAGLFVLAIPPSKYSPEP
                     FAKYPKADVLL"
     gene            542..1309
                     /locus_tag="PLQ30_06275"
     CDS             542..1309
                     /locus_tag="PLQ30_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPW02088.1"
                     /translation="MKNFAFIGLGPFAMSMLERISVISDQIIAADESPERIEHVKEMV
                     SVAFTMNLLDEEAFERAFHEPVDVAIVDIESNGPELLLVTFRLKKLGVPEIIVKSNSE
                     EYEELLRLVGATRVVNSDREAALRVTPLVLSASLTNFMPISGDLVLAEVIAPDFVVGK
                     SVIEGDLRNTRHINVVAVKYASEMDSSGAHKEVFHNLDTKYRFKSGDVLLVTGKESDV
                     FEFSGIPKTIEHEKEATKMTSFLKSMFNRKKKDIENK"
     gene            1471..2649
                     /locus_tag="PLQ30_06280"
     CDS             1471..2649
                     /locus_tag="PLQ30_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HPW02089.1"
                     /translation="MDIFQKCFDFDLDRQAMEKGIYPYFQPLDGLEGTEAVFNGRKLI
                     MIGSNNYLGLTMHPKVKEAARKGLEEYGPSCTGSRFLNGTLKLHNELEERLAAFVGKD
                     AALVFSTGMQTNLGAISSLVDRNDIVIADKDDHASIVDGCRLSMGEMKRYVHADLDHL
                     ERILQKVPSESGAMVIVDGVFSMGGDIADLPGLVKLCEKYGARLYVDDAHSIGVLGDG
                     HGTAWHFGLTDKVDIIMGTFSKSFASLGGFVAGHRDVINYIKHTARSFMFSASLPAPN
                     AMAALAALDIMETEPEHVEHLWDNANFMMKGFRELGFNIGKTQTPIIPVIIGEDEVCF
                     RFWKELFDNGLYTNPVISPAVPEGMALLRTSYMATHTKDQLQRALDIFETAGRKFGII
                     "
     gene            complement(2684..3391)
                     /gene="radC"
                     /locus_tag="PLQ30_06285"
     CDS             complement(2684..3391)
                     /gene="radC"
                     /locus_tag="PLQ30_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPW02090.1"
                     /translation="MTEWQAYEKAELMIKPDTPSLDIRERMLRFGSGSISNLDLVVAI
                     LGSGVPGKPVRKLAKEVLNQITKSSDSCDLDQLMVISGMGEAKSCAVAAALELGRRIF
                     STRGTCIGMPKDAYPLLIHFADIRQEHFIVISLNGGHEVNAIREVTKGLINKTVVHPR
                     EVFADPITDRACAIIVAHNHPSGNLEPSDEDVDITKRLRQSGDILGIPMLDHLIFSES
                     GYFSFMEHGLIVPAKGN"
     gene            3659..4234
                     /locus_tag="PLQ30_06290"
     CDS             3659..4234
                     /locus_tag="PLQ30_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPW02091.1"
                     /translation="MKSIKGTKTEVNLLTGFAGESQARNRYTYFASKAKEEGFIQIQL
                     IFEETANQEKEHAKRLFKFLEGGTAKVSAEFPAGVIGTTRENLAEAAGGENYEWQEMY
                     PSFAKVARDEGFEAIASVFDSIAVAEKQHAKRYEALMANIDEGQVFKRDKPVVWRCLN
                     CGYVYEGTEPPKACPACAHPQAYFELLAENW"
     gene            4253..4627
                     /locus_tag="PLQ30_06295"
     CDS             4253..4627
                     /locus_tag="PLQ30_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HPW02092.1"
                     /translation="MTERKQIYKCMKCGNIVEVLHEGQGQLVCCGEPMKLMVENTSDG
                     AKEKHVPVLEKTADGWWVMVGSILHPMEEKHYIEWIELIADGHSYRAYLSPGQEPKVF
                     FPVKAEKVEAREYCNLHGLWKA"
     gene            4655..4816
                     /locus_tag="PLQ30_06300"
     CDS             4655..4816
                     /locus_tag="PLQ30_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPW02093.1"
                     /translation="MKKYVCDVCGYIYDPAEGDPDGKIPAGTPFEELPDDWVCPMCGA
                     SKDSFSPMD"
     gene            5017..5307
                     /locus_tag="PLQ30_06305"
     CDS             5017..5307
                     /locus_tag="PLQ30_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02094.1"
                     /translation="MISRDDFVFCVGYNGDTAIIDGKAKKEFSGLSTMELAEKGLYRA
                     AFASALYSKNPEEMKAFIEFFNKKAGTSYTEASQLSRLFSVYLETISKAKAL"
     gene            5440..6927
                     /gene="radA"
                     /locus_tag="PLQ30_06310"
     CDS             5440..6927
                     /gene="radA"
                     /locus_tag="PLQ30_06310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPW02095.1"
                     /translation="MKKPSTIYRCSACGHEEPKWLGRCPECGQWNTMVEAKTIGRFEK
                     DKTAFPVPLESVDPALGARVSSGILELDRVLGGGFMRGSAVLLGGEPGIGKSTLLLQA
                     CSKLGAKGKALYISGEESAAQIRLRAERVGALSKQIEVFCGNDMHACLSVMDSAHPLL
                     TVVDSIQTVHSEEAGAVPGTPNQIKFCTQEFVEWAKSHDSIVILVAHVTKDGMIAGPK
                     AAEHLVDAVISFEQAENTLRVLRTSKNRFGSSDELGFFLMGESGLSELQDPSGIFMVR
                     REGQLPSGIAVAIVYEGSRILLAEVQALTIPSKSGIMRVYSDRIDPLRVSRIAAVLEK
                     QTRLDFSSQEIYVNVAGGLRLTEPAVDLPLACALYSARSGQALPEGCALAGELSLAGE
                     IRPVRSMDRRTKAAHQLGFVRIIGPKAMPAGEQLVDVRVTSAGAGKANAGKANAGKAG
                     SSDAERAAKAGAAKTEAATHSAQTLPWLGASSLRESLRILWGQGT"
     gene            6929..>8614
                     /locus_tag="PLQ30_06315"
     CDS             6929..>8614
                     /locus_tag="PLQ30_06315"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPW02096.1"
                     /translation="MQFINNGQRIAGLVVPLLSLRKNNNAACGVFSDLVSLGKIAKKW
                     GLSLIQLLPLNDTGNGPSPYAAISAFALHPIYISLSDSVDTMATFGLPFKDEVAKELV
                     RAENALNRRFGSEARIQHQQVQFEAVLQLKIDALRKAWKASRDICSSLAEAFAARETW
                     AKPYACFVALREEFGLAPWWEWPEWQSILPSDIDRLWFESELAEEERFRLWLQVLAQD
                     QLRRAVQEVRELGIDIMGDIPILLAKDSADVWFQGEIFILDKQAGAPPDMYSPRGQNW
                     GFPLYNWDALRAQNYGFWRQRLQTADNFYTAYRIDHVLGFFRIWAISIHESDAYLGAF
                     KPSRYLELVELKSMGFSDERIAWLSKPHIAEWKIQEAEKKCLDAGISLLADSKDPGAE
                     GKREKKQKEEQEDNQKKEREEGREEERAKRKALFAKSLEELRNYCFLRIKNEPLFLFR
                     PEIRGTAEIDALFGPLWSEFSEAVQILDEYASTMHDWWIDRALYQFEPDRFVFQWSYR
                     ETTSWKSLSPQEQAALEAKAEALSSESQDIWEKRGRELLSMLVSASEMQAFAED"
BASE COUNT         2223 a         1882 c         2300 g         2209 t
ORIGIN      
        1 ggtttcgata ccgtgccaca ggcgaatctc tcaggttcaa gccagcttgc aacaataatc
       61 ctcatgttca ttggcgcttc gcctggctct accggcggag gtatcaagac tgtaacgctt
      121 tatattcttc ttcttgtcgc attgaggatg agagaaggag gagatacgat tgtggaccga
      181 tataggacag tgactcccaa taccgtattc aaagcaataa ctgcgcttgt gaggggcgca
      241 actattgtgt tggtcgccac cacaatcctc ctcgtctctg agagagcagc cgggcatacg
      301 gtaggacttg agactgctat tttcgaatgt gtttctgcgt tcggaacggt tggtctatcg
      361 ataggcatca ccccatatct tacggcagtc tcgaagcttg tcctcattgc ggcaatgttc
      421 atggggaggg ccggcttatt cgtgctcgca attccgcctt ctaaatattc gccggagcct
      481 tttgccaaat atcctaaggc ggatgtgctg ctatgatttg ctgttccaag gaaggtataa
      541 tatgaaaaac tttgcattta ttggtcttgg accatttgcg atgtctatgc tggagcggat
      601 ttcggtaata agcgaccaga ttattgccgc agacgagagc cctgagcgca tcgagcatgt
      661 taaggagatg gtctcggtag cttttaccat gaatctgctc gacgaagaag cattcgaacg
      721 ggcgttccac gagccagtgg atgtagccat agtcgatata gagtcgaatg gtccagagct
      781 cttgcttgta actttcaggc tcaagaaatt gggtgtgccc gaaatcattg taaaatcgaa
      841 ctctgaggaa tatgaagagc ttttgcgact agttggggcg actcgtgtag taaactcgga
      901 cagagaagct gcactgaggg tgactccgct tgtgctttcc gcttctctta cgaattttat
      961 gcccataagc ggggatcttg ttcttgcgga agttatagcc cccgattttg ttgtgggcaa
     1021 gagcgttatc gaaggcgatc tacgcaatac gcgccatatc aatgttgtcg ctgtaaaata
     1081 tgcttcggag atggattcct ccggagctca caaggaagtt tttcataatc tcgatacaaa
     1141 gtatcgcttt aagagtggag atgtgctcct tgtaacgggc aaagagagtg atgttttcga
     1201 gttttctggt attccaaaga caatagaaca tgagaaagaa gcgaccaaaa tgacaagttt
     1261 cttaaaaagc atgtttaacc gcaagaagaa agatatcgaa aataaatagc ggcttttggc
     1321 attgctggcg cgctcgttga ttccattttc tcgaaccatt acaataagga agcggctcag
     1381 gtttgatata cagcctgtgc gctccggcgg caaattgtcg tccggcaaac tgcgttgcag
     1441 aaagtatctg cgcgcgcaat ggaggtccct atggacattt ttcaaaagtg ctttgatttc
     1501 gatctcgaca ggcaagccat ggaaaaaggg atatatcctt atttccagcc gcttgatggg
     1561 ctcgagggaa ccgaggctgt ctttaatggt cgcaagctga tcatgatcgg ttccaacaat
     1621 tatcttggcc tgactatgca tccaaaggtc aaggaagccg ctcgcaaggg tctggaagaa
     1681 tatggtcctt cctgtaccgg aagccgtttt ctcaatggta ctctcaaact tcacaatgag
     1741 ctcgaagagc gcctcgccgc ttttgtcggg aaagatgcgg cactggtttt ttctactggc
     1801 atgcagacca accttggggc gatttcatcg ctggtggatc gcaacgatat cgtcatcgca
     1861 gataaagacg atcacgcctc tatagtggac gggtgcaggc tcagtatggg cgaaatgaag
     1921 cgctatgtgc atgccgacct agaccatctc gaacgtatcc tgcaaaaagt tcctagcgaa
     1981 tcaggggcaa tggtgattgt agatggtgtt ttctccatgg gaggagatat tgcggatttg
     2041 ccaggtctcg taaaactctg cgaaaaatat ggagcgaggc tctatgtaga cgatgcacat
     2101 tccattggtg ttctgggtga tgggcatggt acggcctggc attttggtct taccgataaa
     2161 gtagatatca taatgggcac cttttccaag agttttgctt cgctaggtgg atttgtggct
     2221 ggtcatagag atgtaatcaa ctacatcaag cataccgccc gctcttttat gttttcggca
     2281 tctttgcccg cgcccaatgc catggctgct cttgctgctc tcgatataat ggagacagag
     2341 cctgagcatg tagaacacct ttgggacaat gccaatttca tgatgaaggg cttccgggag
     2401 ctcggcttta atatcgggaa aactcagacg ccgattatcc ccgttattat cggtgaggat
     2461 gaagtgtgct tccgtttttg gaaggagcta ttcgataatg ggctctatac caatccagta
     2521 ataagccctg ccgtaccgga aggaatggcg ttattgcgca cctcctatat ggctactcac
     2581 actaaagatc agctacagcg cgcgctcgat attttcgaga cagccggcag gaagttcggt
     2641 attatctaga caataatata cgcaactcca cttgctaggc tgcctagttt cctttggctg
     2701 gaactatgag accatgctcc ataaaactga aataaccgga ttcagagaag attagatgat
     2761 ccagcatggg tatgcctagg atatcgcctg attgccgaag tcgcttggtt atatccacat
     2821 cttcgtccga aggctccaga ttcccacttg gatgattgtg agccacgatt atggcgcagg
     2881 ctctatccgt gatagggtcg gcaaagactt cgcgcggatg cacgacggtt ttattgataa
     2941 gccccttggt tacctcgcgg atggcattta cttcgtggcc gccatttaac gatataacaa
     3001 tgaaatgctc ttgtcttata tctgcgaaat ggattaggag agggtaggca tccttgggca
     3061 ttcctatgca tgtgccgcga gtcgagaaaa tccgtctccc aagctccaat gccgcagcca
     3121 cagcgcagct cttggcttct cccatccctg atatgaccat aagttgatcg aggtcgcatg
     3181 aatcgctgct ttttgtgatc tggttcaata cctctttggc aagcttgcga acaggcttgc
     3241 cgggaactcc cgaacctaga attgctacga caaggtcgag gttagatatc gagccgctgc
     3301 caaacctcag cattctctcc cttatgtcga gagatggggt atcaggtttt atcattagtt
     3361 ccgctttttc gtatgcttgc cattccgtca ttttgtctcc tattgattat gacgcatcga
     3421 aagtgatcct cgattcaatg tctttcggct caattagcat ctagatagag cataaggagc
     3481 actatggagc actatagagt agcacgcaca taagaatccg cagaaagcat catcagtagt
     3541 ttgatggggc acaacaaatt tgtgtgatcg agcatttgcg tttcaaggcc agctagaata
     3601 taatcaaaag aggtatttgg tttttttgtt aaatctcaag tctcgagagg aggatatgat
     3661 gaaaagcata aagggcacaa agacagaagt aaatcttctt actggatttg caggggagtc
     3721 tcaggcgcgc aatcgctata cctatttcgc ttcgaaggca aaggaagaag gttttatcca
     3781 gatacagctt atttttgagg aaacggcaaa tcaagagaag gagcatgcaa agcgattatt
     3841 taagtttctg gaaggaggaa ccgcaaaagt ctctgcggaa tttccggcag gtgtaatcgg
     3901 caccacgagg gagaatctcg ccgaggcagc aggtggagag aattacgagt ggcaggagat
     3961 gtatccaagc tttgccaaag tggcgcgaga tgagggtttc gaggcgattg caagcgtatt
     4021 tgattccatt gctgtggccg agaaacagca tgctaagaga tatgaagcgc ttatggcgaa
     4081 tatcgatgaa gggcaggtct tcaagcggga taagcctgtt gtttggcgtt gcctaaactg
     4141 cggttatgtc tacgagggaa ccgaacctcc caaagcatgt cccgcatgtg ctcatcctca
     4201 agcctacttc gagcttttgg ctgagaattg gtaataaagg aggctatggt atatgacaga
     4261 aagaaagcaa atttacaaat gcatgaagtg cggtaatatc gtagaagtgc ttcatgaggg
     4321 gcagggtcag ctcgtgtgct gtggagaacc catgaaacta atggtggaga acacgagcga
     4381 tggcgcaaaa gaaaaacacg tgcctgtact tgaaaaaact gccgacggct ggtgggtgat
     4441 ggttggttcg atactacatc caatggaaga aaagcattac atagaatgga ttgagcttat
     4501 cgccgacgga cattcctatc gagcatacct ttcgcctggg caagaaccca aggtcttttt
     4561 ccctgtaaag gcggaaaagg tggaagctag agaatattgc aatcttcacg gtttgtggaa
     4621 ggcataataa agctaaattt taaaggagcg cactatgaag aagtatgtat gtgatgtctg
     4681 tggttacatt tatgatcctg cggaaggcga ccctgacgga aagatacctg ccgggacccc
     4741 atttgaagag ttgccagatg actgggtctg cccgatgtgc ggcgcgagca aggattcatt
     4801 ttcgccaatg gattgaaaaa agagccacct gtctgaaatt tagccgatga tttttgtaat
     4861 ctaggctttt tgcatcataa gacggcggat gctttttgca gcagggggca ataaaaaaga
     4921 gggcacaata aaaaagaggg ggtttgcgct ccctcttttt ttattttata tatcgcatta
     4981 ttcttttatc ttaattttta tgggcttgga gcgtatatga tttctcgtga tgattttgtg
     5041 ttctgcgtag gctataacgg cgacactgca attatagatg gcaaggccaa gaaggaattt
     5101 agcggtcttt ccaccatgga actagcggaa aagggtcttt accgagcagc ttttgcatct
     5161 gcgctctatt ccaaaaatcc cgaagagatg aaagccttta tcgaattttt caacaagaag
     5221 gcaggcacca gctatacgga ggccagtcag ctttccaggc tctttagtgt ctatctcgag
     5281 acaatttcaa aggcaaaggc attatagttc gtgctcgctc gtgcttactt gtgcttgtgt
     5341 ctaccttgat gaatcgaaag cagatcatac gagccattag gcgcgtcgtt gggtacgtct
     5401 attgaacctg cgcctcccct catatacgat aaaaccaata tgaaaaagcc ttcgaccata
     5461 tatcgctgtt cggcttgtgg ccatgaagaa ccaaaatggc ttgggcgttg ccccgagtgc
     5521 ggccaatgga acaccatggt cgaagccaaa acaatagggc gcttcgaaaa ggataaaacg
     5581 gcatttccgg tgccgctgga atcggtggat ccggctctgg gcgcgcgcgt atcgtccgga
     5641 attttagaat tggatcgtgt gctgggcggt ggttttatgc gcggctcagc agtgctcttg
     5701 ggtggtgagc ctgggatagg gaaaagcacc cttcttttgc aggcctgctc gaagctaggg
     5761 gcaaagggca aagcgctgta tatctcaggc gaggaatctg cggcacaaat acgccttaga
     5821 gccgaacgag tcggggctct ttctaagcag atcgaggtgt tctgcggcaa tgatatgcat
     5881 gcttgtctct ccgtgatgga ttcggcgcat ccgctgctta ctgttgttga ttcaatccag
     5941 acagtacatt cagaggaagc gggggcagtg ccaggaacgc cgaatcagat taaattttgc
     6001 actcaggaat tcgtagagtg ggcaaagagc cacgattcta ttgtcatcct tgtggcgcat
     6061 gtgacaaagg atggtatgat agccggtcca aaagcggctg aacatcttgt ggacgcggtg
     6121 atcagcttcg aacaagcgga aaataccctg cgagtgcttc gcactagtaa gaatcgcttt
     6181 gggtcttcgg acgagctggg gttttttctg atgggcgaaa gtgggctttc tgagctgcag
     6241 gatccctctg ggatattcat ggttaggcgc gaaggacaat tgccttcggg cattgctgta
     6301 gcgattgtat acgaaggatc gcggattctg ttagccgaag tgcaggcttt aactattcct
     6361 tcgaaatcgg gcattatgag agtgtattcg gatcgaatcg atcctctgcg tgtctctcgt
     6421 attgcggctg tgctcgagaa gcagacaagg ctcgactttt ccagccagga aatctatgtg
     6481 aatgtcgcag gaggcttgcg gcttaccgag cctgcggtgg atctgccgct cgcctgcgct
     6541 ctctattctg cgcggtcggg gcaagccctg cccgagggtt gcgcacttgc gggtgagctt
     6601 tcgcttgctg gggaaatccg gccggtgcgc tctatggata gaagaacgaa ggcagcccat
     6661 caactcggct ttgtacggat tatcggacca aaggccatgc ccgctggaga gcagctagtg
     6721 gatgtgcgcg tcacaagtgc tggcgctgga aaggcaaatg ccggaaaggc aaatgctgga
     6781 aaggccggtt cttccgatgc cgaaagggca gcaaaagcag gcgcggccaa gaccgaggca
     6841 gcgacccatt cggcacagac attgccctgg ctaggggctt cttctcttcg agaatcgctg
     6901 cgaattcttt gggggcaagg tacttaaaat gcaattcatc aacaatgggc aacgaatagc
     6961 aggacttgtc gtgccgctct tatcgctgag gaaaaataat aatgccgcat gcggagtatt
     7021 ttcggacctg gtaagccttg ggaaaatagc aaagaaatgg ggactttctc tgattcagct
     7081 cctgccgctc aacgatacgg gaaatggacc atcaccatat gctgccataa gtgcctttgc
     7141 tctgcacccg atatatattt ctctctccga ttcggtcgat actatggcta cttttggtct
     7201 gccttttaag gatgaggtag caaaggagtt ggtgcgtgcg gagaatgctc tgaatcgtcg
     7261 atttggaagc gaggcgcgca ttcagcatca gcaagtccaa ttcgaagccg tgctacagct
     7321 aaaaattgat gcacttcgca aggcatggaa ggcgagtcgg gatatctgta gttcccttgc
     7381 agaagccttt gcagctcgag agacctgggc taaaccttat gcctgttttg tcgctctaag
     7441 ggaagaattc ggcttagcgc cttggtggga atggccggag tggcaaagta ttttgccatc
     7501 agatattgat agactttggt tcgagtccga attagcagaa gaggagcgat ttcgactttg
     7561 gcttcaggtg ctcgctcagg atcaactaag gcgagcagtc caagaagtac gtgaacttgg
     7621 catcgatatt atgggcgaca taccaattct ccttgctaag gattcggctg atgtatggtt
     7681 tcagggcgaa atattcatcc tcgacaaaca agctggagcg cctccagaca tgtattcgcc
     7741 caggggacag aactggggtt ttcctctata taattgggat gcactgcgcg cccagaatta
     7801 tggtttttgg cgccaacgcc tgcagactgc agacaatttt tataccgcat atcgcataga
     7861 tcatgtgctg ggttttttcc gtatttgggc cataagtatc catgagagcg atgcttatct
     7921 cggcgccttt aagccttctc ggtatctaga acttgtagaa ctcaaatcta tgggtttttc
     7981 tgacgagcgc atcgcttggt tatcgaagcc acatattgct gaatggaaaa tacaagaagc
     8041 cgagaaaaaa tgcctcgatg cagggatatc gctgcttgca gatagtaagg atccgggcgc
     8101 agaaggaaaa cgagaaaaga agcaaaaaga agagcaagaa gataatcaaa aaaaagagcg
     8161 agaagaaggg cgagaagaag agcgagccaa gagaaaagcg ctatttgcca agagcctcga
     8221 agaactcaga aattactgtt ttttgcgcat aaaaaatgag ccgctctttt tattcagacc
     8281 cgaaatccga gggacagctg agatagatgc actttttggg ccactatgga gcgaattctc
     8341 agaggctgta caaattctcg acgaatatgc cagcacaatg cacgattggt ggatcgatag
     8401 ggcactctat caattcgagc ctgaccgctt cgtctttcag tggagctata gagaaacgac
     8461 aagctggaaa agcctttcgc cccaggaaca ggcggccttg gaggcaaagg ccgaagccct
     8521 cagttcggag tcgcaggaca tctgggagaa acgaggccgc gaacttcttt cgatgcttgt
     8581 atcggcaagc gaaatgcaag cctttgcaga agat
//
LOCUS       DAOMZL010000071         8604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_7735_length_8604_cov_17.876009, whole genome shotgun sequence.
ACCESSION   DAOMZL010000071 DAOMZL010000000
VERSION     DAOMZL010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 8604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8604
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7735_length_8604_cov_17.876009"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLQ30_06320"
     CDS             <1..210
                     /locus_tag="PLQ30_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02097.1"
                     /translation="NLCPSRLSRPVFELDVFESDRGFYPELAIFNFAPNLCPSENLCP
                     SELERFEYIGIFSFESRPCGNLSGR"
     gene            188..826
                     /locus_tag="PLQ30_06325"
     CDS             188..826
                     /locus_tag="PLQ30_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02098.1"
                     /translation="MGIYLGDDIFIHAASTGAKTGVIRSSLRERSWQQRFLFAGRALP
                     ASGFSGFALDWGSGISFAQLDMLLQGDLLAFVRGISGWTEVSLPVANNLSAGLRAGVQ
                     WDRVLGVVRVPIELDLGRISGVSVFAGPAITFGLPTLNGRLYTQGDFFLATGGLRWSS
                     FFYSRGAHRLGSYIELRYDYYVPKQGQVESIRSDLQSCLSISIGLRLRNVFY"
     gene            complement(820..1647)
                     /locus_tag="PLQ30_06330"
     CDS             complement(820..1647)
                     /locus_tag="PLQ30_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HPW02099.1"
                     /translation="MLTTILLTIGFSAALAFILGVALGFFNEKFKIELDPKISEVRQA
                     LPGANCGACGYPGCDGYSEAVATGRAPTTRCSVGGSATAEVLSKIMGIEAGTVEEKVA
                     VLLCQGAIDKTVNKGEYSGIPTCRAAKLSTGGIKACSWGCQGFGDCTLVCKFDAIEMG
                     KDGLPHIDYDNCTGCGLCVEECPQKILTLIPLTKVGSVVLCSNRTVLKQTVLKNCKVG
                     CIKCDLCVRSCPEGAISMVNGIPVTDYAKCNSCGICVQKCPTKCYKLLEKDVLVVHQ"
     gene            complement(1660..2235)
                     /locus_tag="PLQ30_06335"
     CDS             complement(1660..2235)
                     /locus_tag="PLQ30_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HPW02100.1"
                     /translation="MDLGKILLLSMLVNNIVVMRFIALCSYIGMTSDLGQSMGMGYAV
                     TFVTVLATAATWPIYHYILLPLDLAFLQILVFILVIASLVQLVEFYLKKNIPGLYAAM
                     GIYLPLITTNCAICAVTFENITNNYNFIQSIVYAIGVSLGYLLAMILLAGIRDRMKTS
                     PVPKFLQGTPILFMATSLIGIAFMGFAGLIK"
     gene            complement(2235..2876)
                     /gene="rsxE"
                     /locus_tag="PLQ30_06340"
     CDS             complement(2235..2876)
                     /gene="rsxE"
                     /locus_tag="PLQ30_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HPW02101.1"
                     /translation="MKKLYSIFKQGIIFDNPLLMLMIGLCSAIAVTTNVSNGIGMGLA
                     MTFVLLFSEIIISLFRKLIPSMIRIPIFIIVIAAFTTIVDLVMKAYFPDLSKSMGVFI
                     PLIVVNCIIMGRVEAFASKQDLLSVIADALGMGLGYTWVLVGLAAIRELLGNGTFAGI
                     QIMPASYEPILFFILPPGGFFVFSLFISLNIWLKKKMEAAARRKELVSKKEAA"
     gene            complement(2879..3598)
                     /locus_tag="PLQ30_06345"
     CDS             complement(2879..3598)
                     /locus_tag="PLQ30_06345"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPW02102.1"
                     /translation="MKKTKLGVILMLYASIACASLAIVYAFTKDSIERQSEIQLQTSL
                     KEIFPQASTFELVEDLTSPEPNIKFEAVYEVKSDAVPLGLAIKASGPSYGGNATLLVG
                     IDLHRSIAGVRIVALNDTPGLGMNASNPTYYVDKIKKVTFLGQFEGKYIADPFEVKKD
                     VEAITAATITSKSLAKIIKTAGDAAIAYMDQKVMMASMEVEKPNGETSIVAPPTASTG
                     FPIGSSEGKSKEKIPPQAGGK"
     gene            complement(3595..4605)
                     /locus_tag="PLQ30_06350"
     CDS             complement(3595..4605)
                     /locus_tag="PLQ30_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPW02103.1"
                     /translation="MSDKKIILSSSPHTFSQVDTPRIMISVVIALVPAAAYGVYLYGL
                     AALGVIVTSITSAVLSEFLFRKLIRASTTIGDFSAVVSGFLLALVLPPGTPLWMVALG
                     AAFAIVVAKEFFGGLGANPLNPALIGRAFLLMSFPAAITSWNMPKGLALPADAVSAAT
                     PLNLIKQGNSLADVAKYFGASDIGAFYRQLFMGYRPGSIGESSILLVLLGGLFLLAIG
                     VIQWIVPVSVLASTFVFSWLLGMDPLLSLLTGGIAFGAFFMATDYSTRPMTEKGQAMY
                     GIGIGLIAVLIRKFGGYPEGVTYAILIMNILTPFLNKLRTKKYGFAHPSKPVKPSKEA
                     RK"
     gene            complement(4595..5956)
                     /gene="rsxC"
                     /locus_tag="PLQ30_06355"
     CDS             complement(4595..5956)
                     /gene="rsxC"
                     /locus_tag="PLQ30_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HPW02104.1"
                     /translation="MSMRTFRGGAHPSERKDRSQHAPIEKVNSVPQVVIPVNQHFGAP
                     IQPLVKVGDFVRVGQKIADAEGRMTVPLHASIAGVVKKIEPRMQSNNADGLCIIIEPK
                     DSPAGESDREFMPPLDPFACSKEEALSRIREAGIIGMGGAGFPAHVKLAPTKPISVVI
                     ANAAECEPYLTIDERTLLEMPDKIIDGLSIVMKIINAAEGVVAIEENKKHTLPLVEKA
                     IQENPQVKAGMKIGIQVLKTKYPQGGEKMLITAITGRQVPSGGLPMDVGCVVSNVGTL
                     CAIADAFREGKPLIERGFTVTGGACRTPKNLVVPVGTVVADLAKTGIIDIDEESLSKV
                     IFGGPMMGVAVPSSEIPVQKNTSGVVLMSVEEALQFEEKTCIRCGRCIRACPMRISPA
                     LLAIAIESGNYSEAEKIGLQDCIECGACSFVCPARRYLVQRFRIGKQVLRALKQKEAQ
                     NVR"
     gene            complement(6273..6995)
                     /gene="jag"
                     /locus_tag="PLQ30_06360"
     CDS             complement(6273..6995)
                     /gene="jag"
                     /locus_tag="PLQ30_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HPW02105.1"
                     /translation="MIYEFEGKTEREAMELATQELGLDTSSFDVEIIENQSGGLFKKG
                     KVRIRVHTKEATSEDNREKESNEHNKFGEREEARDERHIEPLPMEDFENKIIEWTSEV
                     ISLMGYSAQVSVAFREPNKLGLRIDTEAASILIGKKGRNIDSLQLLANVYAGNLGHSD
                     TKIVLDSENYRLRREEALVRVAYKTAEEVRSTGHSILLEPMNPFERRIIHTTLNDIVD
                     IETKSEGDGLMKQVRVMLKGRN"
     gene            complement(7028..>8604)
                     /gene="yidC"
                     /locus_tag="PLQ30_06365"
     CDS             complement(7028..>8604)
                     /gene="yidC"
                     /locus_tag="PLQ30_06365"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPW02106.1"
                     /translation="RMFEAKMAGSDEKVPFIFKKVFSFRDGEYLFGLAISIEQPEGKP
                     IFLGTNGVAYRIDLGPQIGPRFDQMPKNADYRKYIAEIDGKKKKQNLKANTSDTIAPN
                     ASWVALSGKYFTFIAIPQAPISAYEIATAQDPLINQTNSLGLLRSTFSGSSTTDTYYF
                     YFGPKTSAELGKYEYADKNGFGLSNLNLEDAMEGSGILGWLETILKFLLNVFYRLIPN
                     YGIAIILVTVLIKLLFYPLTKKGSLATARMSELQPKMQELQAKYKNNPQKLNQEMAEL
                     YKREHYNPMSGCLPMLIQFPLFIAMYNLFNNHFELRGAMFIPGWINDLSLPESLVNFG
                     NFRIPLLGWNDLRALPIIYLFSQLMYGKFTQAPQSTQGNNPQANQMKLMMYGMPIMFF
                     FILYDVPAGLLIYWITNNILTIAQQILINNLMKKRKMAGAAAQAAEGTPAAGAANAGP
                     RLATGKSSAVDTKAKTVGRVPAKEGFSERVTNWLEKQAGEAEKASGSAYTKKKDQGRT
                     SGSDRSSAPRKNKAKK"
BASE COUNT         2203 a         2131 c         1886 g         2384 t
ORIGIN      
        1 aacctctgtc cctcccgtct ctccaggccg gtgtttgaac tcgatgtctt tgagtctgac
       61 cgcggcttct atcccgagct cgcaatcttc aattttgccc caaacctctg tccctccgaa
      121 aacctctgtc cctccgaact tgagcgattc gagtatattg gcattttctc tttcgaaagc
      181 cgaccatgtg ggaatttatc tgggagatga catttttatc catgctgcga gcactggagc
      241 taagacagga gttattcgca gttcactgag ggaaagaagc tggcaacagc gatttctgtt
      301 tgcggggcgc gcgcttcccg cttctggttt ttctggcttt gccttagact gggggagcgg
      361 aatcagcttt gctcaactgg atatgcttct acagggcgat cttttggcct ttgtgcgcgg
      421 aatctctggg tggacggagg tttcgcttcc cgttgcaaac aaccttagcg ccggtctaag
      481 ggcaggcgtt caatgggatc gtgttcttgg tgtggtaaga gttcctattg agcttgacct
      541 tggccggatt tctggagtct cggtgtttgc cggtccagca attacatttg gattgccaac
      601 gctgaatggc aggctgtaca cccagggaga tttctttttg gctacaggag ggttgagatg
      661 gtcgtctttc ttctattcaa ggggtgctca tcgacttggt agctacattg aactcagata
      721 cgattattat gttcctaagc aaggccaggt cgaatcaatt cggtccgatc ttcaatcttg
      781 ccttagtatt tcgattggtc ttcgcttgcg caatgtattt tattgatgga ctactagcac
      841 atctttctca agcaacttgt agcactttgt cggacacttc tggacacata tgccgcaaga
      901 gttgcacttg gcataatctg tcacgggaat gccattcacc atcgaaattg cgccttccgg
      961 acaagaacga acacatagat cgcacttgat gcatccgacc ttgcagttct tgagtacggt
     1021 ctgcttgagc actgtcctat tcgagcagag caccacggaa ccgacctttg taagcggtat
     1081 gagcgtcaat attttctgtg gacactcctc tacacatagg ccgcagccgg tgcaattgtc
     1141 ataatcgata tgaggaagcc catcttttcc catctcgatg gcatcgaact tacagacgag
     1201 cgtacagtca ccaaatccct ggcatcccca gctacaggcc ttaataccgc ccgtagatag
     1261 cttggcagcc ctacaggtgg gaataccgct gtattcgccc ttgtttactg ttttatcgat
     1321 cgcgccttgg cataatagaa cagctacctt ctcttcgacc gttcctgcct cgatgcccat
     1381 aattttcgaa agcacttcag cagtggctga tccaccgaca gaacacctgg ttgtgggggc
     1441 gcgaccagta gccaccgcct cggaataacc atcgcaacct ggatagccac atgccccgca
     1501 gtttgccccg ggcaaagcct gtcttacctc gctgatcttt ggatctagtt cgatcttgaa
     1561 tttttcatta aagaagccta atgccactcc aaggataaag gcgagcgctg cagaaaatcc
     1621 tattgtcaat aaaattgtgg taagcatctc gatatcctct tattttatga gccctgcgaa
     1681 acccataaac gctattccaa tcaaggacgt tgccataaaa agtatcggcg ttccttgtaa
     1741 aaacttcggt acagggctcg tcttcattcg atcccttatg ccagcaagga gaatcatcgc
     1801 caagagataa ccgagcgaga cgccaattgc gtacacaatt gattggataa agttgtaatt
     1861 attcgtgata ttctcgaaag taaccgcgca aatagcacaa tttgttgtta taagtggcaa
     1921 atagataccc attgctgcgt acaaaccggg aatgttcttc ttaagataga attcgacgag
     1981 ctgaaccagg cttgcaatga caagaatgaa taccagaatc tgaagaaaag ccaaatcaag
     2041 cggcagaaga atatagtggt aaataggcca tgtcgctgct gtagcgagca ctgttacaaa
     2101 ggttactgca taacccattc ccatggattg ccccagatcg cttgtcattc ctatatatga
     2161 acagagtgca ataaagcgca ttaccacgat attgtttacc aacatcgaca aaaggagaat
     2221 tttgccaaga tccattatgc agcctccttc ttagaaacca attcttttct tctcgcagct
     2281 gcctccatct tcttcttgag ccatatattt aaggaaataa acagactgaa aacaaagaaa
     2341 cctcctggtg gcaaaataaa gaagagaatc ggttcatagg aggcaggcat tatctgaata
     2401 ccggcaaaag tcccattccc aagcagctct cttattgcag caagaccaac aagaacccag
     2461 gtataaccga ggcccattcc cagcgcgtct gcaatcaccg ataataaatc ttgcttagat
     2521 gcaaaggctt cgacacgccc cattattatg cagttgacga cgatcaatgg gatgaaaaca
     2581 cccatgctct tcgaaagatc gggaaagtag gctttcatca ctaagtcaac gatcgttgta
     2641 aatgctgcaa tgactatgat aaaaatcggg attcgaatca tagatggaat cagcttgcga
     2701 aatagagaga ttattatctc cgaaaacaga agcacaaaag tcatggccaa gcccatgcca
     2761 ataccgttcg agacattagt cgtcaccgca atggcagaac atagaccgat catcagcatc
     2821 aatagaggat tatcaaaaat gatcccttgt ttgaaaatag aatacagctt cttcattctc
     2881 acttgcctcc tgcttggggc gggattttct ctttagattt tccttcggat gagcctatgg
     2941 gaaatcctgt ggaagccgta ggaggtgcca ctatagatgt ttcgccattt ggcttttcga
     3001 cttccatcga ggccatcatc accttctgat ccatataggc tattgcggca tcgccagcgg
     3061 tcttgatgat cttggcaaga gacttcgatg ttattgtggc cgccgtaatc gcctcaacat
     3121 ccttcttcac ctcgaaggga tcagcaatat atttcccttc aaattggcct aagaacgtca
     3181 cctttttgat tttatccaca taataagtcg gattagatgc attcattcca aggcccggcg
     3241 tatcattgag cgctacaatc cttactcctg ctatgctgcg atgcaaatca attccaacaa
     3301 gcagggttgc attgcctccg tagcttggtc cggaggcttt tatagccaat cccagtggaa
     3361 ccgcatcgct ctttacctca tatactgctt caaatttgat attcggctct ggacttgtaa
     3421 gatcttcgac cagctcgaag gtacttgctt gtggaaagat ctccttaagg gaggtctgca
     3481 gttgaatttc gctttgtctt tcgatgctat ccttggtaaa ggcgtagaca atggcaagac
     3541 ttgcacaggc aatcgaggca tatagcataa gaatgacgcc aagttttgtc tttttcattt
     3601 tctagcctcc ttggaaggct taacaggctt tgaaggatgc gcaaagccat attttttagt
     3661 tcgcagctta tttaaaaatg gcgtcaaaat attcataatc aatatcgcat aggtcactcc
     3721 ctcaggatat ccgccgaatt ttcttataag aacagcgatc aggccaatac caataccata
     3781 catggcctga cccttctccg tcattggcct tgtagaataa tccgtcgcca taaagaatgc
     3841 gccaaatgct atgccacctg tcaacaagct cagcaatgga tccattccta aaagccaaga
     3901 gaacacaaat gtggatgcta acacggatac cggcactatc cactgaatga ctccgatggc
     3961 aagaagaaaa agcccgccca agagcacaag caaaatcgag ctctctccga tggagccggg
     4021 gcggtatccc ataaacagtt ggcgatagaa tgccccgata tcgctcgctc cgaaatactt
     4081 ggctacatca gctaatgaat tgccctgctt gattaagttc aatggtgtag ccgcactgac
     4141 tgcgtctgcg ggcaatgcaa ggcctttcgg catgttccag ctagtaatcg ccgcagggaa
     4201 gctcatgagt agaaaagcgc ggccaatcag tgcgggattt aagggatttg cgcccaatcc
     4261 accaaaaaac tcttttgcaa ccacaatggc aaaggcagca cccagcgcaa ccatccagag
     4321 cggagtacct ggaggcagaa ccagcgcaag tagaaacccg cttacaacag ccgaaaaatc
     4381 accaatcgtg gtgctggctc ttattagctt tcgaaataag aattccgata acacggccga
     4441 tgtaatcgaa gtcacaatga ctccaagcgc agccaggcca tagagataaa caccataggc
     4501 cgcggctggc accaacgcta ttacgacgga gatcataatg cgcggagtat cgacctgcga
     4561 gaaggtatgg gggctcgaag ataggataat tttcttatct gacattctgt gcctccttct
     4621 gtttcagcgc acgaagtacc tgtttcccaa tcctgaagcg ctggacaaga taacgacgag
     4681 ctgggcacac aaatgagcag gcgccacatt cgatgcaatc ttgaaggcca atcttttcag
     4741 cttcgctata attgccgctt tcgatcgcaa tagcaagtaa agcaggacta atcctcatag
     4801 ggcatgctct aatacaacga ccacaacgaa tacaggtttt ttcctcgaac tgcaatgctt
     4861 cttcgacact catgagaacc acgcccgaag tgttcttttg taccggtatt tcgctgctcg
     4921 gtacagccac acccatcatt ggtccgccaa agatcacttt ggaaagcgat tcctcatcga
     4981 tatcgattat tccggtttta gcaaggtctg ccaccacggt tccaacgggt actacaaggt
     5041 tctttggggt cctgcacgct cctccggtca cggtaaatcc tcgctctata agtggcttgc
     5101 cttccctaaa cgcatctgca attgcacaca atgtcccaac attagatacc acacagccga
     5161 catccatagg gagacctccg gacggtacct gtctgcctgt gattgctgtg ataagcattt
     5221 tttcgccgcc ctgtggatat tttgtcttaa ggacttgtat accgattttc attccggcct
     5281 tgacctgcgg attctcttgg atggctttct ctaccagtgg aagcgtatgt ttcttatttt
     5341 cttctattgc tacgacacct tcggctgcat ttatgatttt catcactatc gaaagaccat
     5401 cgatgatttt atccggcatc tccaagagag tacgttcgtc aattgtcaaa taaggctcac
     5461 attccgctgc atttgcaatg accacagaga ttggctttgt aggagcgagc tttacatgag
     5521 ccggaaagcc tgcgcctccc ataccaatga ttcctgcttc gcgaatgcgc gagagcgcct
     5581 cttccttact gcatgcgaag ggatcgagcg gaggcatgaa ttccctatcg gattctcctg
     5641 ctggtgaatc ctttggctca attatgatac atagaccatc tgcattgtta gattgcattc
     5701 gcggctcgat tttctttacc acgcctgcta tcgatgcatg gagaggtacg gtcatgcgac
     5761 cttccgcatc tgctatcttc tgcccaaccc tcacaaaatc tcccaccttg actagtggtt
     5821 gaattggggc accaaaatgc tgattgacag gaatcacaac ctgcggcacc gaattaacct
     5881 tctcgattgg cgcatgctgc gagcgatcct ttctctcgga cggatgtgcc ccgcccctga
     5941 atgtcctcat tgacatagtg gaaaggtctc ctttgaagat ttgctttgac ctcgcgaaga
     6001 atccaatcgt cttcgcattc aatgtaaaat gatagaaaat tataacaaaa tcgagctata
     6061 ttagcaccag ttttcaattg tggtcaaggc aaaaagagca taagtattcg ctatacaata
     6121 aaaagataaa aaattattta atattggtcc gaaaaattga gatgaaacat gacaacgaaa
     6181 tatctcggca aaaaaagata gcgccgccat gctgtgctgc ggcgccttat tcgtatcagc
     6241 cagattgtta tgccagatcc acggaatgct tactaatttc ttccctttag cattacgcgt
     6301 acctgcttca taaggccatc tccttcgctc ttcgtctcta tatcgacgat atcattgagg
     6361 gttgtatgga taattcgacg ctcgaaggga ttcataggct caagcaagat ggaatgccca
     6421 gtactgcgca cttcctctgc ggttttatag gcgactctga ctaaggcctc ttcacgcctc
     6481 aagcggtaat tctcggaatc gagcacgatc ttggtgtcgc tatgcccaag attgccagca
     6541 tagacgtttg ctaaaagctg cagcgaatcg atgttcctac cttttttccc tatcagaatc
     6601 gaggcagcct ctgtatctat gcgcaagcca agtttgttgg gttccctaaa tgccacggag
     6661 acttgagcac tatatcccat caagctaatt acctcactcg tccactcaat aatcttgttc
     6721 tcgaaatcct ccattggaag gggctctata tgcctctcat cccgggcttc ttctctttcc
     6781 ccgaatttat tgtgctcatt ggattctttt tctcgattat cctcagaggt tgcttctttg
     6841 gtatgaaccc gtatgcgcac ctttcctttt ttgaataagc caccactttg gttttcgatg
     6901 atctccacat cgaatgaact tgtatccagg ccgagctcct gagtggctag ttccatagcc
     6961 tcgcgctcgg tctttccttc aaactcatat atcatttgga tcgctcctga ttgaaagatt
     7021 caatggttta ttttttggcc ttgttttttc ttggtgccga ggatctgtcg gaacctgagg
     7081 tgcggccttg atctttcttc tttgtataag cagaaccgct tgctttttct gcctcgccag
     7141 cctgcttttc cagccaattc gtcactctct cagaaaaacc ctcttttgcc ggcactctac
     7201 caacggtctt cgcttttgta tctaccgccg agctctttcc tgtcgctaat cttggaccag
     7261 cattcgctgc tccagcggct ggcgttcctt cggccgcctg tgctgctgca ccagccatct
     7321 tgcgtttttt catcaaatta ttgattagga tctgttgagc aatggtcaga atattgtttg
     7381 taatccaata aatcagaagg ccagcgggca catcgtagag aatgaaaaag aacattatag
     7441 gcatgccata catcatcaac ttcatctgat tagcctgcgg attgttgcct tgggtgcttt
     7501 gaggggcctg ggtaaacttg ccatacatta attggctaaa taggtatatg atgggcagtg
     7561 cccttaaatc gttccatcca agaagaggaa ttcggaaatt gccaaagttt acaaggctct
     7621 caggcaggga cagatcattg atccagcctg gtatgaacat tgctcccctg agctcgaagt
     7681 gattgttgaa aagattgtac attgcaatga ataaggggaa ttgaatgagc attggcagac
     7741 atccagacat tggattgtag tgttccctct tatatagctc ggccatttcc tgattgagct
     7801 tctgtggatt gttcttatac ttggcctgca attcttgcat cttgggctgg agttccgaca
     7861 ttctcgccgt agcgagcgac cctttctttg taagtgggta gaatagcagc tttatcagca
     7921 cggtaacgag aatgatggct ataccataat taggaattag gcgataaaaa acattgagca
     7981 ggaatttcaa tatggtttcc aaccatccaa gaatgcccga gccttccatg gcgtcttcca
     8041 aatttaggtt ggaaagacca aatccgtttt tgtccgcata ctcatatttt cccaactccg
     8101 cggatgtctt gggtccaaaa tagaaatagt aagtatcggt agttgaggaa ccagagaagg
     8161 tgcttctcaa aagccccaga ctattggtct ggttaataag cggatcctgg gcggttgcta
     8221 tttcgtaagc cgatattggt gcttgtggaa tagcgataaa tgtaaaatat tttccggaca
     8281 aggctaccca gcttgcgttt ggcgctatgg tgtctgaggt attggccttg agattctgtt
     8341 tctttttctt gccatcgatt tctgcgatgt attttcgata atcggcattc ttgggcatct
     8401 gatcgaagcg cggaccaatc tgtggtccaa ggtcgatcct ataagcaact ccatttgttc
     8461 ccagaaaaat gggtttgcct tcgggctgtt cgatgctaat tgcgagccca aatagatatt
     8521 ctccatcgcg gaatgaaaag accttcttga atataaaggg gaccttttcg tcgcttcctg
     8581 ccatctttgc ctcgaacatc ctcg
//
LOCUS       DAOMZL010000072         8085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_8391_length_8085_cov_24.539975, whole genome shotgun sequence.
ACCESSION   DAOMZL010000072 DAOMZL010000000
VERSION     DAOMZL010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8391_length_8085_cov_24.539975"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..1331)
                     /locus_tag="PLQ30_06370"
     CDS             complement(279..1331)
                     /locus_tag="PLQ30_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HPW02107.1"
                     /translation="MKAALFTQFREPIEIVEVPDPIAPDDGVVLKVDATGICRSDWHG
                     WQGHDPDIRLPHVPGHELAGTIVEVGRDIKNWKLSERVTMPFVAGCGHCPPCLSSNQQ
                     VCDNQFQPGFSAWGSFAEYVAIRYADMNLVRLPESLDAATAASLGCRFATAFRALEAQ
                     SRLRAGEWIAIFGCGGVGLSAIMIATAMGARIIAIDIQEEKLKKAKEIGADIVINSRE
                     VSDIVAVIRDITHGGVQVSMDALGSPETCFSAISSLAKRGRHVQVGLMLADHKHPRIP
                     MDLVIARELEIYGSHGIQAHRYDVLLGMIEAGKLHPERLIEARLNLRQGVDFLQKMDQ
                     FPGVGISVITSMQDNS"
     gene            complement(1328..1891)
                     /locus_tag="PLQ30_06375"
     CDS             complement(1328..1891)
                     /locus_tag="PLQ30_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HPW02108.1"
                     /translation="MKELYRSDNGRCIIAVGDITNIACDAIVNAANSSLLGGGGVDGA
                     IHSAGGPAILAECRALRNSSLPNGLPPGKAVATTAGQLPARRVIHTVGPIWQGGAYGE
                     DETLASCYRECLALAVHEQLKSIAFPAISTGIYGFPKERAAKIAWNTVQDYLASTPEA
                     ASITVWFVFFSEIDAHIFLEANGKIKL"
     gene            complement(1940..2572)
                     /locus_tag="PLQ30_06380"
     CDS             complement(1940..2572)
                     /locus_tag="PLQ30_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW02109.1"
                     /translation="MGKFSNKVRTFSALEVAKLCGVVNQTAINWIRSGYLKAFNTPGG
                     QYRVYAEDLVSFLKERDMRVPDELKDDLTMPVDSGLAIIVDDDKDLNTILKRLLERRI
                     DDLRVAQAYDGFEAGRIIAERRPAIVILDLNLPGVDGLSLCKKIRSDESFGQPAVIAI
                     TGIGEEDAQPRMMEAGADAFFSKPLNFDALIAKTKELMEARKDLEKKDGN"
     gene            2728..4911
                     /locus_tag="PLQ30_06385"
     CDS             2728..4911
                     /locus_tag="PLQ30_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HPW02110.1"
                     /translation="MDDTIDFDPTRIENLCVDEIKIAERIANELAIRLLQVQAVLNLS
                     AEGCTIPFISRYRKERTGNLDELQVRDCIQKFQSYKNLEERRLEVIKGISAMGKLDAF
                     LHENILRATTLTELEDLWSPFKKKKKTRGMLAQEKGLAPFAELMERTTILEEVVAAAT
                     SFIHRNEEHPELSVESAEDALSGARDILAERLSQDTSLRAHIKRVFIKEGELAVKGIG
                     DEEKRGQSTYQMYWDYREPLSTLKHHRVLAINRGEREGELDVSIEVSDELVETRMLER
                     VRPANAQHKQAVIDGLLRLLLPAVRREIRSDLTESAESHAIEVFSTNLRNLLMQPPLR
                     GTRVLGIDPGIRTGTKCAALDETGKFLDYFVINQETKPEQGKKDVAAAVIKYALSVIA
                     VGNGTGSHEVQKLIAESIAENGLSCRFAVVDEDGASVYSASDLAREEFPNLDLTIRGA
                     ISIGRRLQDPLSELVKIDPKSIGVGLYQHDVNQKQLASQLDDVVGSVVNQVGVNLNTA
                     SYSLLRYVSGINASLARKIVKFRDASGVIRNRYLLKEIPGLGEKTFEQCAGFLKIPES
                     DNALDNTWVHPENYAVASEILSMVKSGKEPQTKERESLKQKYKIGDITLDDIIGELKK
                     PNRDPRDDLPPPILQEGVLSLEDLRPGMRVKGKVKNVVDFGAFVDIGIKENALIHVSE
                     MAEHFVKNPMEVLKVGDVKDFTVISIDAVRRRIGLSLKANLKVDS"
     assembly_gap    5100..5199
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5423..6529
                     /locus_tag="PLQ30_06390"
     CDS             5423..6529
                     /locus_tag="PLQ30_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW02111.1"
                     /translation="MPILFEPFLGNTLVARGPTNLGLYVFKQSDVARAIVIDSGGDED
                     AGRKILRECERLGVELAMIINTHSNADHCGGNAFLSKRTGCSIAATELEAAFLSYPQL
                     EPSFLSGSFPQKALRNKFLMAQPSRANCLLEPPCALTFDKDGRWQLSKPAQVHGSAAA
                     NTSQGSPINELQSASTFAPWSAPTSASAIIPKNESQSAAQGMPCLQIMSLPGHYFSMI
                     GILTPDNVFFVADSLAGAPILEKYGLFFLYDIEAELATLSMLESSEAEWFVPAHAEPT
                     QDIKSLVDINRAKIFEIADVIVSAVNSEANCAEGATLENVMAAVFSHYDIVLNANQYV
                     LVGSTIRSYLFWLCDQGKLENAFEHQRMIFRIKR"
     gene            6526..>8085
                     /locus_tag="PLQ30_06395"
     CDS             6526..>8085
                     /locus_tag="PLQ30_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02112.1"
                     /translation="MNRKNSRFGFGKISMAIFSLLILKFAMGSGGSGAVYAQGHLAVE
                     LTHPVYMMIETAELRGVISRLSSVKPYTSQQVADFIAILMGHMEAFSPAEQDLIRKYA
                     EEFKIGKAIEESLWRDRSGIAQAGIRVEEATKLDVAGICDLIDGSSSTPFKDVWHFNS
                     RWVPYLKGDPLSWLSFKGEAGFTFDKIERDLYLPYEFTKEWDSNHIWFGTPRTSDGTL
                     DYPTASYDIHEDIAAETDDSSVMVRLSRFRRDWGMGSGSFSLSSTARPFVGFETVLRP
                     SKYFAISGLVGSLTNWQKDDNEKSTAIEYYDKNGDGVLDIEDGDYFYYSALSYQKMLG
                     LQRMELFPFDWLTISATSTIVGAKRFELGYFSPLLFSVMYQNQLADIDNLAVQVDGQI
                     VVPRIGKFYASFYADEMEITNLSELFTKARNMFALQGGAKIPLPWWSFATLTAQYSKI
                     EPFVYAHYPTWYPDYRLRVDTSYTQDGENLGYYLPPNSDEFLVKLDMMPAPDWRLSLK
                     YSFVRHGDNPGN"
BASE COUNT         2037 a         1836 c         1996 g         2116 t
ORIGIN      
        1 acccgcttca cgagcttggg aagctcgggt aataccggta tacgacggtc gcagtggtgt
       61 gttcgcgcgc catcggggta cgacggtggc gattaaatgc gcgccaggtc attgttttat
      121 gaaaaaaaat ttctaaaaga catgccctat tgatgcgctt atctactata gcgagcgccg
      181 caagctttcg tcaaggcgag ccagcccatc acttttgagc attagtaaaa aacatcttgt
      241 ttatcctgat aaatcttggg aattctgtca aaatcttgct aggaattatc ttgcatcgat
      301 gtgatcacgc taatgcccac gcctgggaat tgatccatct tctgcaaaaa atcaacgccc
      361 tggcgtaggt taaggcgagc ttcgatgagc cgctctgggt ggagttttcc agcttcgatc
      421 attccaagaa gcacatcata acggtgggct tgaatgccat gactcccgta gatctcgagc
      481 tcgcgcgcaa tcacgaggtc cattggaatt cggggatgtt tgtggtcagc gagcatgagg
      541 ccaacttgca catgccgacc gcgcttggca aggcttgaga tggcgctaaa gcatgtctct
      601 ggactaccaa gtgcgtccat agaaacctgt acgccgccat gtgtgatatc gcgaattaca
      661 gcaacaatat cagatacttc tcggctattg attacaatat ccgcgccaat ttccttggct
      721 tttttaagct tctcttcttg aatatcgatt gcgatgattc gagcgcccat cgctgtggca
      781 atcatgatcg ctgaaaggcc cacaccccca caaccaaaga ttgcgatcca ttctcccgcg
      841 cgaagtctgg attgtgcttc cagcgcccgg aaagcagtag caaaacgaca gcccaggctt
      901 gccgccgttg ctgcatcgag gctctcaggc aagcgcacaa gattcatatc tgcatagcga
      961 atagccacat attctgcgaa ggagccccac gccgagaatc ccggttggaa ttggttgtcg
     1021 cagacctgct gattggagct aaggcagggt ggacagtggc cgcagccagc aacaaagggc
     1081 atagtcactc tctcgctgag tttccaattc ttgatatctc tgccgacttc aacaatagtg
     1141 ccggcgagtt catgcccggg cacatgtgga agccttatat caggatcgtg cccctgccag
     1201 ccgtgccaat cactgcggca gatgcccgtt gcatcgactt tgagaactac gccatcgtca
     1261 ggcgctattg gatcaggcac ctcaactatc tcaataggct ctctaaattg tgtgaatagt
     1321 gcggctttca tagctttatt tttccattgg cctcaagaaa gatatgcgca tctatctccg
     1381 aaaagaatac aaaccatact gttatggagg cagcttcagg ggtactcgct aaataatcct
     1441 gtaccgtatt ccacgcgatt ttggctgcgc gttcttttgg gaatccatag ataccggttg
     1501 agatggcagg aaaggcaata gacttgagct gctcgtgcac agcgagcgca aggcactcgc
     1561 gatagcagga cgccagtgtt tcgtcttcgc cataggcccc tccttgccaa atcggcccta
     1621 ccgtatggat aacgcgcctt gcgggaagct gaccggcggt agttgccact gcttttcccg
     1681 gaggcaatcc atttggtaag gagctgtttc gcaacgcacg acactcggcg aggattgctg
     1741 ggcctccagc gctatggata gctccatcga caccgccccc tcccaggagg ctcgagtttg
     1801 ctgcgttgac aattgcatcg caagcgatat tggttatatc tccaactgca attatgcagc
     1861 ggccattatc agatctatac agctctttca taattggagt ctatcctctt cgatatggac
     1921 tttttcaata taatgttatt caattgccat ccttcttttc tagatctttg cgggcttcca
     1981 tgagttcctt tgtttttgct atcaaagcat cgaaatttag cggtttagaa aagaatgcat
     2041 cggcaccagc ttccatcatg cgaggttggg catcctcttc tccaattccc gtaatagcta
     2101 tcaccgccgg ctgaccaaag gactcatccg atcggatttt tttgcagagc gaaaggccat
     2161 ctacaccagg aagattcaga tccaagatga ctatagccgg acggcgctcc gcgatgattc
     2221 ggcctgcctc aaagccatcg taagcctgtg cgacgcgcag gtcgtctatt cttcgctcga
     2281 ggagtcgttt aagaattgtg ttgagatctt tgtcatcatc tacgattatc gcgagtccac
     2341 tatccaccgg catagtaagg tcgtctttaa gctcatccgg aacgcgcata tctcgttctt
     2401 tgagaaaaga aaccagatct tcggcataaa ctcgatattg tcctccgggc gtattaaagg
     2461 ctttgagata gccacttcgt atccaattta tcgctgtttg attaacaaca ccacagagct
     2521 ttgcaacttc gagcgcggaa aatgttcgaa ccttattgct gaacttcccc atcaggtgcc
     2581 tctttaattc ataacggtat attcagctca atgcagagag ttatcattac aaggatacac
     2641 catatatggt tagagatcca gtgaatcatc atgcctggaa tcttgacctt gatatttttt
     2701 tgaacttccc ataactttag gctcgccatg gatgacacaa tagattttga cccaaccaga
     2761 atagagaatc tctgcgtaga tgagattaag attgcggaga gaatagcaaa tgagcttgct
     2821 atacggcttt tgcaagtaca agcggtactg aatctttcag ccgaaggatg cactattccc
     2881 tttataagtc gatatcgcaa ggagcgaact ggcaatcttg acgagttaca agttcgcgat
     2941 tgtatccaaa agttccaaag ttacaaaaat ctcgaagagc gcaggctcga agtaataaaa
     3001 ggtatctcag ctatgggtaa gctcgatgct tttctacatg agaacattct gcgcgctact
     3061 actctgacgg aactcgagga cctctggtca cctttcaaga aaaagaagaa aacacgcggc
     3121 atgctcgctc aagaaaaggg actagctcct tttgcggagc tgatggaaag aaccaccatc
     3181 ctggaggaag tggttgctgc tgccacttct tttatccatc gaaatgaaga acaccccgag
     3241 ctttccgtgg agagcgctga ggatgcgctt tcgggagcgc gagatatcct tgccgaacgg
     3301 ctttcgcagg atacaagctt gcgcgcccac ataaaacgag tatttatcaa ggaaggagag
     3361 cttgcggtaa aaggtatagg cgatgaggaa aagcgagggc aatccactta tcagatgtac
     3421 tgggactatc gagaacctct ctcgactctc aagcatcata gagtgcttgc gataaatcga
     3481 ggagaacgag aaggcgagct cgatgtgtcg atagaggtga gcgatgaatt agtcgaaacg
     3541 cggatgctag agcgtgtgcg accagccaat gcacagcata agcaagcagt aatcgacggg
     3601 cttttgcgcc ttcttttgcc agcagtgcga agagagatcc gctcggatct caccgaaagc
     3661 gcggaaagcc atgctataga agtgttcagc accaatctac gcaatcttct tatgcagccg
     3721 cccttgcgtg gtacaagagt cttgggcatc gatcctggaa ttcgaactgg tacaaaatgt
     3781 gcagcgctcg atgaaaccgg taagttcttg gattactttg ttataaacca ggagaccaag
     3841 cccgaacagg gcaagaagga tgtcgcggca gcagtgataa aatatgcgct ttcagtcata
     3901 gcggtgggga atggtactgg aagccatgag gttcaaaagc tgatagcgga atcgattgca
     3961 gaaaatgggc tttcatgccg ctttgcagtc gtagatgagg atggcgcgtc tgtgtattcg
     4021 gcaagtgatc ttgcgagaga agaatttcca aatctcgatc ttacaatccg aggggcgatc
     4081 tcgatagggc ggcgtctgca ggatcctctt tcggagctag ttaaaatcga tccaaagagc
     4141 attggagtag ggctatatca gcacgatgtc aaccagaaac agctggcgag ccagctcgac
     4201 gatgttgttg gatcggtagt gaaccaggtt ggagtcaatc taaacaccgc aagttattcg
     4261 ctattgcggt atgtatcggg cataaacgcg agcttggcaa ggaagattgt caagttccga
     4321 gatgcaagcg gcgtgatacg gaatcgttat ctattaaagg agattccagg gcttggcgag
     4381 aagacgttcg agcagtgtgc cggctttctc aagataccgg agagcgataa cgcgctagat
     4441 aatacttggg ttcaccctga aaattatgca gttgcctcag aaattttgtc catggtgaag
     4501 tctggcaagg aaccgcaaac caaagaaaga gagagtttga agcagaaata taagattggt
     4561 gatattactc tggatgacat aattggcgaa ctaaagaaac caaatcgtga tccacgagat
     4621 gaccttccgc cgccaattct gcaggaaggt gtgttgagcc tcgaggatct ccggccgggc
     4681 atgagagtaa aaggaaaagt gaagaatgtc gtcgatttcg gtgcctttgt cgatatcggc
     4741 ataaaagaga acgcacttat ccatgtctct gagatggcgg agcactttgt taagaatcca
     4801 atggaggtgc taaaggtggg tgatgtaaag gactttacag ttatcagcat cgatgcggtg
     4861 cggcggcgca tagggttgtc gctcaaggca aatttgaagg ttgatagctg actactgcat
     4921 ggtacacgca ccacgcagaa taaggcggaa atggccgctg cgatttaact tcggctagat
     4981 tttagttttt tgccaatggc ttattttcgg ccagaatgct ctttaggcaa tggcctattt
     5041 tcacccagaa tactctttag gcaatgcgaa atcttgaatt ctttgtctga ctttgatatn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cagaatactc tttaggcaat
     5221 gcgaaatctt gaattctttg tctgactttg atataatcta aaatgccttc gtgcgattgc
     5281 tcggtgtcgg tttttgtgcc gctaatcccc caaccccagc ggccaaccct acccccgatg
     5341 ccgacattgc atgagggctt ttttttgagc cctgctagcc agaaagcttg ctgaagaaca
     5401 ataaaaagag taacaataac ctatgccaat tctattcgaa cccttcttgg gcaatactct
     5461 tgttgcgcgt gggccaacca atcttggact ctatgttttc aagcagagcg atgtcgctcg
     5521 cgcaattgtt atagattctg gtggcgacga ggatgcagga aggaagattc tgcgcgaatg
     5581 tgagcggctt ggtgtcgagc tcgcaatgat tatcaacact cattccaatg cggatcactg
     5641 cggtggaaat gcattcctgt ctaagcgaac tggttgtagc attgcagcaa cagaactgga
     5701 ggcggctttt ttgagctatc ctcagctcga gccttcgttt ttatctggca gttttccaca
     5761 gaaagcgctg aggaacaagt ttcttatggc acagccttcg cgagcaaatt gcttgctcga
     5821 gcctccttgt gctttgacct tcgacaaaga tggcaggtgg cagctttcca agcccgcaca
     5881 ggtacatggg agcgcagctg caaatacatc acagggttca cctataaatg aattgcagag
     5941 cgcttcgaca ttcgcgcctt ggagcgctcc tacaagtgcg tcagcaatca tacccaaaaa
     6001 tgaatcacag agtgcggcac aaggcatgcc ttgtttgcag attatgtctc tgccaggcca
     6061 ttattttagc atgattggaa ttctaacccc agacaatgtg ttttttgttg cagattctct
     6121 tgccggtgca ccaattcttg aaaaatatgg ccttttcttt ttgtatgata ttgaggcaga
     6181 acttgcaacg ctttcgatgc tcgagtcgag cgaggctgaa tggtttgtac cagctcatgc
     6241 agaaccgact caagatataa aatcacttgt ggatataaat cgggctaaga ttttcgaaat
     6301 tgctgatgtc attgtctcgg cggtaaattc cgaggcaaac tgtgcagaag gggctacgct
     6361 cgaaaatgtt atggctgccg tattttctca ttatgatata gtccttaatg ctaaccagta
     6421 tgtacttgtt ggatctacca ttcgctcgta tcttttctgg ttatgcgatc aaggaaagct
     6481 agaaaatgct ttcgaacatc aaagaatgat ttttaggata aagcgatgaa ccggaagaat
     6541 tctaggtttg gttttggcaa aatatctatg gcaatatttt cgctgttgat tttaaagttt
     6601 gctatgggta gcggtggatc cggggctgtg tatgcacagg gacatctagc ggtcgagctt
     6661 acacatcctg tctatatgat gattgaaacc gcggagcttc gcggagtcat ttcaaggctc
     6721 tcttctgtaa agccctatac tagccagcag gttgccgact tcattgcgat tttgatgggg
     6781 cacatggaag ctttttcgcc agcagaacag gatctgattc gcaagtatgc tgaggaattc
     6841 aaaataggca aggccataga ggagtctctg tggagggaca ggtctggcat tgctcaggct
     6901 gggattcggg tagaggaggc tactaagctc gatgtagcgg gcatttgcga tctcatcgat
     6961 ggctcgtctt ctacgccttt taaggatgtt tggcacttca attcccgctg ggtaccgtat
     7021 cttaagggcg atcctctgtc atggctttct tttaagggag aagccgggtt tacatttgat
     7081 aagatcgagc gggatcttta tttgccttat gaatttacaa aggaatggga ttcgaatcat
     7141 atatggtttg ggactcctcg tacttcagat ggcactctcg attatcccac agcgagctac
     7201 gatatccatg aggacatagc ggcggagaca gacgatagtt cggttatggt gcgcctctcg
     7261 agattcaggc gggattgggg catgggttcg ggctctttct cgctttctag tacagcgcgg
     7321 ccttttgtgg gtttcgagac tgtgctaagg ccttcgaaat attttgcaat ctctggactt
     7381 gtgggttctc ttacgaattg gcagaaggat gacaacgaaa aaagcacagc tatagagtat
     7441 tatgataaaa acggcgacgg ggttctcgat atagaagatg gcgactattt ctattactcg
     7501 gctctttcct atcagaagat gctcggcctc cagcgcatgg aactttttcc cttcgattgg
     7561 ctcacaattt ctgcgacgag cactattgtc ggggctaagc gcttcgagct cggttatttt
     7621 tcgcccttgc ttttctctgt gatgtaccag aatcagcttg ccgatatcga taatctcgcg
     7681 gtccaggtgg atggccagat cgttgtgccg cggataggta aattctatgc atccttttat
     7741 gccgacgaga tggaaattac caatttgtcg gaactcttta ccaaggcgcg gaatatgttc
     7801 gctttgcagg gcggtgcaaa gattcctctt ccatggtggt cttttgcaac cctgactgcg
     7861 caatacagca agatcgagcc ttttgtgtat gcgcattatc caacctggta tccagattac
     7921 cgtttgcgag tcgatacttc ctatactcaa gatggagaaa atttaggcta ttatctaccg
     7981 cccaactcag acgaattctt ggtcaagctc gacatgatgc cagcgccaga ctggcggctt
     8041 tctctcaaat atagttttgt tcggcatggg gataatccag gcaat
//
LOCUS       DAOMZL010000073         7969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_8554_length_7969_cov_28.176017, whole genome shotgun sequence.
ACCESSION   DAOMZL010000073 DAOMZL010000000
VERSION     DAOMZL010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7969
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8554_length_7969_cov_28.176017"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLQ30_06400"
     CDS             <1..302
                     /locus_tag="PLQ30_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02113.1"
                     /translation="IKEINAAQKEQAEKAMSSEVFTVYWMLKEEGLLNAEALANSLQE
                     VFAKYPYWHNSERYEREIRRSLYKALTQAGIQDVTRIPAIAQNIIKILKTGQAVQ"
     gene            299..727
                     /locus_tag="PLQ30_06405"
     CDS             299..727
                     /locus_tag="PLQ30_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HPW02114.1"
                     /translation="MTVKAQPMTTRAFKEEVLTLAKIVNVTPKEIHVQSMKHKWASCS
                     NKGRLTFDKSLLDQPDEVRLKIILHELLHLKYPNHGKMFKSLLATYLARLSQIIDPNL
                     GLGCGNPVALSTIKEGDGVLNLESGAGFDAFLAAKKVGHS"
     gene            797..1075
                     /locus_tag="PLQ30_06410"
     CDS             797..1075
                     /locus_tag="PLQ30_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HPW02115.1"
                     /translation="MTTNEEKELRQSIENGEWTTVPNFDEIKQNLTKAAAETAMKDFR
                     INVRISKRDVEALKTKALEDGIPYQTLVTSILHKYVTGRLKEPATDKE"
     gene            complement(1244..2635)
                     /gene="argH"
                     /locus_tag="PLQ30_06415"
     CDS             complement(1244..2635)
                     /gene="argH"
                     /locus_tag="PLQ30_06415"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPW02116.1"
                     /translation="MGKLWQTSKGKELDPIIEQFLSSIESDKRIVFEDIECSQAHAIM
                     LGEQGIIPLQDASLLVDELAKIRKELEAGSLAIDLCAEDVHSFLEEELTRRLGDVGKS
                     IHAGRSRNDQVAVAFKLHVRNACDRCEKLVKKAIGACLDLAEQHIDTLMPGYTHLQRA
                     QPVSFAHHLLAWCAALERDALRFHDAGLRADESPLGAGALAGSGLPIDRARTASLLGF
                     ARTSMNTMDAVADRDAAIEYAAAAATLMMHLSRFCEDMCLWASSEYGFITISPRASTG
                     SSIMPQKRNPDPAELIRGKAGRVFGSLQALLTMEKGLPYAYDRDLQEDKSLFFDIEDT
                     IEGSLKAFAVLVQSIEPNIVTMRVALDEGYVEATDIAEFLVKKGIPFRTAYKAAKQLV
                     EHCTHEKKSLREIVQADLAVHDSFEKAAIDARELTEYLDPVACVARRSQIGGPAPARV
                     AEQIERLRRFIAP"
     gene            complement(2642..3919)
                     /locus_tag="PLQ30_06420"
     CDS             complement(2642..3919)
                     /locus_tag="PLQ30_06420"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPW02117.1"
                     /translation="MKKKIVLAYSGGLDTTVIVPWLKEHYDCEVIAVCGDVGQEADFD
                     AIGARALASGASKFIKLDQKEEFVKEYLWSLVKAGTPYEKKYLLGTSAARPLLAKGLT
                     ETALAEGADAIAHGCTGKGNDQVRFELGVKAFAPEMEVIAPWRIWDIQSREEEIAYLE
                     AHGIPVPMKKSDSYSRDDNLWHISHEGLDLEDPSNEPHFEGMLKMCVTPEKAPDKPEY
                     VTLAFEHGVPVSINGERLDPVSIVRKLNKIGGANGVGIVDMVENRVVGMKSRGVYETP
                     GGTIIMEAHDRLEQLCLDKKTLSFKMTVAQRFAEILYEGEWFSPLCTALRAFIDSTQN
                     VVAGQVMLKLYKGNMIFAGSKSPYSLYDASLASFTTGPLFSHNDSTGFINLFGLPTKV
                     RARLNERIKKYGIAASPDVVKPGNSGYTAHAGD"
     gene            4218..5270
                     /locus_tag="PLQ30_06425"
     CDS             4218..5270
                     /locus_tag="PLQ30_06425"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPW02118.1"
                     /translation="MIYRKLLLSVVALLVAILPVTAFTPKKEYTMQVNVTQVVDWGAG
                     AQKFADLVAQKTNGVINIKPYWSSSLLAGKQTNWYQAVADGAIDFATESTMNACNVIR
                     PFNLFSLPFFINTYDNVDKIENGKSGARIIAEMEKGGVKFLAWGENGFRQITNSKREI
                     RTPKDVEGLKIRVVGSPIMIDIFKQLGADPINMNWGDAVTAFQQGVVDGQENPKGVLI
                     GNKIWEYHKYMTIWNYLIDPLVFCVNSQVWKSFPPDIQAQIQEAALEAGEWEKALARC
                     YLDGDKSLKIFKEKFKDPRDPENLFVVAPAKGMKITVLTADEIAAFKEKTKPVYDKWV
                     PTIGEDIYKLALEDMK"
     gene            5361..5876
                     /locus_tag="PLQ30_06430"
     CDS             5361..5876
                     /locus_tag="PLQ30_06430"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPW02119.1"
                     /translation="MKKNRIRIDELMSAIFLAIMAIIAFVNVINRYFFHRGFAFTEEI
                     TVNLFVWITLLGIAIAFRRGANLKMTNLFDKFSPNLKRVALIISAFLGVMIFSFLIYN
                     SAREIYKNMTFYHAFSEALGIPTWIYSLGTPIFSLFVIKEIIASAIKEIKALKTSLPS
                     GNSDNPKKEAQ"
     gene            5876..7168
                     /locus_tag="PLQ30_06435"
     CDS             5876..7168
                     /locus_tag="PLQ30_06435"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPW02120.1"
                     /translation="MAALLVFGVFILLIIIGVPIATSLGTAAIGAIMNYDLGMSMISR
                     NFEANIAKFPLVAIPFFILAGVLMDKSKIVDGIARFLILLTGKRRGGLAIAAVIACIF
                     WGAVSGSGPATAAALGLVFITPMIKQGYNKYFAATTIAAGAGLSIIIPPSISFIVYGQ
                     ITSVSVGALFLGGIIPGVLVGIFLIGVVYIISRKNNYRGAEESGTPKEILKAFVDSLW
                     ALFAPVIILGSIYLGIATPTEAAIMGVFYALFVGIFIYHTLTIDVIYQALVDTVVSSA
                     VVMFVVSMAGIFSWAAATLGVIDAAANLISSITTNQLVFMFLTMIVMIIAGMFLDAIS
                     ITNVFMPMIMPALLALHIDPLYFGICLTVSLAIGQITPPVAVNLYVTANLIHSNINNE
                     MVKFVLPMVYASVLALILIVLIPQLSLFIPVISKLYIP"
     gene            7186..>7969
                     /locus_tag="PLQ30_06440"
     CDS             7186..>7969
                     /locus_tag="PLQ30_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HPW02121.1"
                     /translation="MSETKNPFPNRFDSLLVEEKMKMLETMIRIRTFETHVEELFMNK
                     KIFGFVHLYIGEEAIATGVMANLRTDDYITSTHRGHGHAIAKGANTSRMMAELFGRKT
                     GYCKGKGGSMHIADFSIGMLGANGVVGGGFNIAVGAALSSQIKKTDQVAVCFFGDGAS
                     NRGTFHEALNLASIWKLPVLFVNENNQYASTTPVSYAVSAQHVADRAKGYGIEGYTVD
                     GNNVAEVYDAANYLVGRAREGKGPALLECLTYRVKGHFVGDPE"
BASE COUNT         2217 a         1847 c         1816 g         2089 t
ORIGIN      
        1 taatcaagga aattaacgct gcccaaaaag agcaggcaga aaaggcaatg tcatctgagg
       61 tcttcaccgt ttactggatg ctcaaagaag aaggcctgct caacgcagaa gcactggcaa
      121 atagcctaca ggaagtattt gcaaaatacc cgtactggca caatagcgaa cgttatgagc
      181 gagaaatcag aagaagcctc tataaggcat taacacaggc aggcatacag gatgtaacaa
      241 ggattccagc catagcacag aatatcataa aaatattaaa aacagggcag gctgtgcaat
      301 gaccgtaaaa gcccaaccaa tgaccacgag agcatttaaa gaagaagtac tgacgctcgc
      361 taaaattgtt aatgttaccc caaaagaaat tcacgtccaa tcaatgaagc acaaatgggc
      421 aagctgctca aacaaaggca gactcacctt cgacaaaagc ctcctcgacc aacccgatga
      481 agtccggcta aagatcatcc tacatgagct tttgcatctc aaatacccaa accatggtaa
      541 aatgttcaaa tcgttgcttg ccacttacct tgccagactc agccaaatta tcgatcctaa
      601 tctgggactt ggttgtggaa atccagtggc tctttcaacc ataaaagaag gcgatggagt
      661 cctaaattta gaaagtggtg ccggttttga tgcttttctt gctgccaaaa aagttggcca
      721 tagttgagtg cacgttatac ggacgctttc ttcccgagta ggagatatac aaagcaatat
      781 acgagaagaa agaaagatga ccacaaatga ggaaaaggaa cttaggcagt ctatagaaaa
      841 cggtgaatgg actactgtcc ccaacttcga cgagattaag cagaatctta cgaaagcagc
      901 cgctgaaact gcaatgaagg actttaggat caatgtccgt atctctaaac gtgatgttga
      961 agcattaaaa actaaagcac ttgaggatgg tattccttac cagacgctgg tgacgagcat
     1021 tctccacaag tatgtaacgg gaagactcaa ggagcctgca acagataagg aataacatcg
     1081 atagtctaca atcgagttag ccatttggaa attgaaaaag gtcaatagat gaaagacgat
     1141 gaaatgaagc gaattgttaa aacaaagtat tcaagaattg caaaaagtgg ttgaaaaagg
     1201 aactctgcta gtgagatttc ttgataagat ccaacatgtg ccctcaagga gctatgaatc
     1261 ttcgcaggcg ctcgatctgc tcggccacac gagctggcgc aggcccgcct atttgggatc
     1321 ggcgtgcaac acaggcaaca gggtcaagat attccgtaag ctcccttgca tcgatcgcgg
     1381 ccttctcaaa ggaatcatgg actgcaagat cagcctgcac aatttcgcgc aggctctttt
     1441 tttcatgcgt gcagtgttct acaagctgct tagccgcctt ataggcagtg cggaaaggaa
     1501 ttcctttctt tactagaaat tcggcgatat ctgtcgcttc gacatagccc tcatcgagcg
     1561 ctacacgcat cgtaacgata tttggctcaa tgctttgaac aaggaccgcg aaggctttga
     1621 gcgaaccctc tatcgtatct tcgatatcga aaaaaagcga tttatcctcc tggagatcgc
     1681 gatcgtaggc gtagggcagc cccttttcca tggtgagcag ggcctggagg ctgccgaaca
     1741 ctctgcctgc cttgcctcga atgagctctg cgggatcagg attgcgcttt tgcggcataa
     1801 tgctcgagcc ggtgctcgca cgaggcgaaa ttgtaatgaa cccatactcc gaagaagccc
     1861 atagacacat gtcttcacag aagcgcgaaa gatgcatcat gagtgtcgct gcggctgctg
     1921 catactctat ggcagcatcg cggtcagcaa cggcgtccat tgtgttcata ctcgttctgg
     1981 caaatcctaa gaggctcgca gtgcgcgctc ggtcgatagg caatccgctc cctgcgagcg
     2041 ctccagcacc gagcgggctt tcatctgctc gcaggcctgc atcgtggaat cgaagcgcat
     2101 cgcgttctaa cgcagcgcac catgccagaa gatgatgagc aaagctcact ggctgggctc
     2161 tttgcaggtg agtgtagccc ggcatcagtg tatcgatatg ctgctccgca aggtcgaggc
     2221 aggcgcctat cgcctttttt acgagctttt cacacctatc gcacgcattt cgcacatgga
     2281 gcttaaaagc aaccgccact tggtcgttac ggcttcggcc tgcatgtatg gatttgccaa
     2341 catcgccgag cctgcgcgtc agttcctctt cgagaaagct atgtacatct tcggcgcata
     2401 aatcgatggc caaagagcct gcttcgagct ctttgcgaat ttttgccaat tcgtctacga
     2461 gcaagctcgc atcctgcaat ggaattatcc cctgctcgcc aagcatgata gcatgtgcct
     2521 gcgagcattc tatatcttcg aatacaatgc gcttatccga ctcaatgctg gaaagaaact
     2581 gttcgattat tggatcgagc tctttgcctt tgctggtttg ccagagtttc cccatgaaat
     2641 atcagtctcc tgcatgggca gtatagcccg aatttcctgg tttgacgacg tcgggggagg
     2701 cagcaatgcc atacttcttg attcgctcat tgagtcgtgc cctgactttt gttggcaaac
     2761 cgaagagatt gatgaagcct gtcgaatcat tgtgagagaa caaaggccct gtggtaaagc
     2821 tagcaagact tgcatcataa agcgaatagg gagattttga gccggcaaaa atcatatttc
     2881 ccttatatag cttgagcata acctggccag cgactacatt ctgggtcgaa tcgataaaag
     2941 cccgcagagc ggtacaaaga ggactgaacc actctccttc atagaggatc tctgcaaaac
     3001 gctgggccac tgtcatcttg aatgagagtg tctttttgtc gagacaaagc tgctctaatc
     3061 tgtcgtgtgc ttccataata atggtaccgc ccggcgtttc gtacacaccg cgcgatttca
     3121 tgcccaccac gcggttttcc accatatcga cgatgccgac tccgtttgct ccgccgatct
     3181 tgttaagctt ccggacaatc gatactggat cgagtctctc accatttatc gaaacaggaa
     3241 caccatgctc gaatgctagg gtcacatatt caggcttgtc tggagctttt tcaggagtaa
     3301 cacacatctt gagcatgcct tcgaaatggg gctcattcga agggtcttcc agatcgaggc
     3361 cttcatggct gatatgccag agattgtcat cgcggctata ggaatctgac ttcttcatcg
     3421 gcactggaat tccatgagct tcgaggtagg ctatttcctc ttctcgactc tgaatatccc
     3481 atatacgcca tggagcgatg acttccattt caggggcaaa ggccttgact cccagctcga
     3541 agcgaacctg atcgttgccc ttccccgtgc agccatgggc aattgcatcg gcgccctctg
     3601 caagagcagt ttcggtgaga cctttggcaa ggagcgggcg ggctgcactc gtaccgagga
     3661 gatacttttt ttcgtatgga gtacctgctt ttacaagaga ccataagtat tctttgacaa
     3721 attcttcttt ctgatcgagt ttgataaact tcgatgctcc cgaagcaagc gctcgagcgc
     3781 caatcgcatc gaaatcagcc tcttgaccga catcaccgca gaccgctatc acctcgcagt
     3841 cataatgctc cttaagccag ggtactataa ccgtagtatc cagtccgccg gaatatgcaa
     3901 gcacgatttt ctttttcata agtctaattc tcccttgaat aaatatgtaa aatataacta
     3961 ttgtatcaag accttgggac ttatctcctg agggcgcttt tattcatata ttggctagct
     4021 ttgagcaaat tcaagaaagc acaacgtgaa taagttcatc ttcttgaaca tattatactc
     4081 agacataata ttttatgcaa tatgttatgc atgaatatgt agcaaatttt gatcgatact
     4141 aaatatgcct tatttttaaa taaataaaca ttgacagaat aaatattggg agcgaaaatt
     4201 gcgctcagga ggttcatatg atctatcgta aattattgct ttctgttgta gcacttcttg
     4261 ttgcaatcct accagttacg gcatttactc ctaaaaaaga gtatacgatg caagtaaatg
     4321 taacccaagt tgtggattgg ggtgctggcg cacaaaaatt cgctgatctg gttgcccaga
     4381 agacaaacgg agttatcaat ataaagccat attggagttc gtctcttttg gcgggtaagc
     4441 agaccaactg gtatcaagcg gtagcggatg gagccataga ctttgctaca gaatcgacga
     4501 tgaacgcctg caatgtaatt cgtcctttca atctcttcag cctgccattc ttcatcaata
     4561 cttacgataa tgtcgacaag atagaaaatg gcaaatccgg tgcgcgaatc atagctgaaa
     4621 tggaaaaagg cggagtcaag ttccttgcat ggggagaaaa cggtttccgc cagatcacca
     4681 attccaaacg cgaaataaga actcctaaag atgttgaggg gctcaaaatc cgtgttgttg
     4741 gatcgccgat aatgatcgat atcttcaaac aactcggcgc agatccaatc aatatgaact
     4801 ggggagatgc ggtaacagct ttccagcaag gtgtagtcga cggtcaagag aatccaaaag
     4861 gtgtcttaat cggcaataag atatgggaat accataaata tatgactatt tggaattatc
     4921 tgatcgatcc gctagtcttc tgtgtaaatt cccaggtatg gaagtctttc ccacccgata
     4981 ttcaggctca gatacaagaa gcggctctag aagctggaga gtgggaaaaa gcgcttgcta
     5041 gatgctatct cgatggagat aagtcactca aaattttcaa agaaaaattc aaggatccca
     5101 gggatccaga gaatctattt gttgtagctc ctgccaaggg aatgaagatt accgttctca
     5161 cagctgacga gatagcggcg ttcaaagaaa aaacaaaacc tgtatacgac aagtgggtac
     5221 ccacaatcgg cgaagatata tataaacttg cactcgaaga catgaaataa caagccttac
     5281 gccgcaacat gcaggtaccg tacagtacct tctattctcg gccggcctct aaggccggct
     5341 tttttataag gagaaccata atgaagaaga ataggatacg tatcgatgaa cttatgagtg
     5401 ctatttttct agcaataatg gcgatcatag ctttcgtcaa tgtgatcaac cgatatttct
     5461 ttcacagagg ttttgcgttt acagaggaaa tcactgtcaa tctatttgta tggatcaccc
     5521 tcttaggaat cgcaattgcc tttcgacgtg gcgctaatct taagatgaca aacctattcg
     5581 acaagttctc gcccaatcta aagcgagtgg cattgataat atctgcattc ctcggggtca
     5641 tgatattctc ttttcttatc tataattccg cgcgagaaat ctataagaat atgactttct
     5701 atcatgcatt ttcagaagcc cttggtattc caacatggat atatagcctt ggtacaccta
     5761 tattttcgct attcgtcatt aaagaaatca tcgcaagtgc catcaaagaa ataaaagccc
     5821 ttaagacctc tcttccttca ggcaattcag ataatcccaa gaaggaggct caataatggc
     5881 ggcgcttttg gtattcggag ttttcatcct cctcattatc ataggggttc cgattgctac
     5941 atcgcttggt accgcggcaa tcggtgcaat aatgaattat gatcttggaa tgtccatgat
     6001 atcgagaaat ttcgaggcca atatcgccaa attccccctc gtcgcaatcc cgtttttcat
     6061 acttgccggg gtgctcatgg acaagtcgaa aatagtcgat ggcattgcgc gctttttaat
     6121 attgctcact ggaaagaggc gtggcggatt ggcaatagcc gcagtgatag cttgcatttt
     6181 ctggggagct gtctcgggct caggtccagc gactgccgct gcacttgggc ttgtattcat
     6241 aactcccatg ataaagcagg gctacaataa atatttcgca gcaactacca ttgctgctgg
     6301 cgcggggctt tcgatcatca tacctccttc gatttccttc atagtctatg gccaaataac
     6361 cagtgtatct gttggagctt tattcttggg cggtattatc ccaggggtat tagtgggaat
     6421 tttccttata ggagtagtct atattatatc gagaaaaaat aattatcggg gagcggaaga
     6481 atctggcact ccaaaggaga tattaaaagc ctttgtcgat tcgctttggg cgctttttgc
     6541 tcctgtcata attctcggtt cgatatattt gggaattgct acacctacgg aagcagccat
     6601 catgggagta ttctatgccc tattcgttgg aatattcatc tatcatacct tgacaatcga
     6661 tgtgatttat caggctctgg tagatacggt ggtttcctct gcagtggtca tgttcgtggt
     6721 ctcgatggct ggaatattct cttgggcggc agccacacta ggcgtcatcg atgcagctgc
     6781 caatctcatt tcgagcataa caacaaatca gctcgttttc atgttcctca caatgatcgt
     6841 catgatcatt gctggaatgt tcctcgatgc aatctcgata accaacgtat ttatgcctat
     6901 gataatgccc gctttgcttg cgctccacat cgatccgctc tattttggaa tttgcctcac
     6961 agtctcgctt gccatcggtc agattacacc gccggttgca gtgaatttgt acgtcaccgc
     7021 taatctcatc catagcaaca tcaacaatga gatggttaaa ttcgtgctcc ccatggtgta
     7081 tgcatctgtt cttgctctta ttcttattgt attgatacca cagctttctc tattcattcc
     7141 cgttatctcg aaattataca taccatgagc ataaggaggt gctcgatgtc agagacgaaa
     7201 aatccctttc ctaatcgctt tgattctttg ctagtcgaag aaaagatgaa gatgctcgag
     7261 acaatgattc gcataaggac attcgagact cacgtagaag agcttttcat gaacaaaaaa
     7321 atcttcggct ttgtgcatct ctatatagga gaagaagcca tcgcaacagg agtgatggct
     7381 aacctacgta ccgacgacta cattacctcg acgcaccgcg gccatggcca tgcaatcgcg
     7441 aaaggcgcga ataccagcag gatgatggct gaactatttg gcagaaagac cggctactgc
     7501 aaaggcaagg gtggctcaat gcatattgcc gatttctcta tcggcatgct tggagcaaat
     7561 ggtgtcgtag gcggtggatt caatatcgcg gtgggagccg cgctttcttc gcagataaag
     7621 aagaccgatc aagtcgcggt gtgcttcttt ggcgacggag catcgaatcg aggcacattc
     7681 cacgaagcgc tcaatttagc atcgatctgg aaattgccag tgctctttgt caacgaaaac
     7741 aatcaatatg catcgacaac gccagttagt tatgcggttt cggcgcaaca tgtagctgac
     7801 agagcaaagg gttatggcat agagggttac acagtcgatg gcaacaatgt cgccgaagtt
     7861 tacgatgctg ccaattatct tgtagggaga gcaagagaag gaaaaggccc tgctctccta
     7921 gaatgtctta catatcgagt aaaaggccac ttcgtgggag accccgaac
//
LOCUS       DAOMZL010000074         7736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_8911_length_7736_cov_15.545762, whole genome shotgun sequence.
ACCESSION   DAOMZL010000074 DAOMZL010000000
VERSION     DAOMZL010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8911_length_7736_cov_15.545762"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLQ30_06445"
     CDS             <1..317
                     /locus_tag="PLQ30_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPW02122.1"
                     /translation="RVAQETGKYLVFFDDDEFARAPDNILGCTVLHQEELAYRSLKAA
                     IEEKLQFGKAEIVGMKEGYIEFLNKNPSYIKNVPESIRIKVDAVIQSIKSGRLTFQVP
                     SL"
     gene            328..1899
                     /locus_tag="PLQ30_06450"
     CDS             328..1899
                     /locus_tag="PLQ30_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW02123.1"
                     /translation="MLAVRMQGIYRRYYPRGVHANDGASLEVEKGSIHAIVGENGAGK
                     TTLMKILAGLDQPDEGNIEIEGKFVKIESPSKAQALGIGMVHQHFLMFDELSAAENIF
                     FGIEPHRNNKVSKIAGIVDSQELNARTLQLATEYGFAIDPSAPAACLSIAARQQIEIL
                     RQLARHTKILILDEPSSVLTEQETKVLFEKLSEIKRLGHTIILVTHKIHEAMQIADRV
                     TVMRGGKTVGTYDISETTLEELTSLIMGKSFIEATQKPIEKPQIKNTQKKPIVLHIDG
                     LNVRRHQHYGVGLSDISFEVHAGEILGISALSGNGLDELEDVLAGFRSPSSGTCTFDG
                     HNVELHNSRRYQSLFQKRLIAYLPSDRIRRGIAPSLSVRDNFIALSRQDFFPKGWILE
                     NAAKQAIVNAIHSFEINALPDQPARELSGGNIQKLSIARLFSRDLPRLFILCEPTWSL
                     DIRSAEHTYRRIRNARERGTAILLLSSNIDEILELSDYVMVLSRGRRVLYQENGNWLN
                     RAVIGEYLLGNRIHE"
     gene            1896..2930
                     /locus_tag="PLQ30_06455"
     CDS             1896..2930
                     /locus_tag="PLQ30_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW02124.1"
                     /translation="MRNQGASSTALKVLLAATLSIILATTVLLLFAADFRSALWQFFV
                     EPFSSRYFFGNYIADTVPLIIAGLGVSIAFRSRNFNLGGEGQLYAGALAGAVLALHIS
                     EAFGPSAVWILVAFIGAFIGAMIGTISGLLKAYWQVSEMISSYLLSAIIINISDFFIT
                     GLLQDRTSNFQTTAQIPSEFLLPRIFAPSKLDISIWFALFLILFFQILSKKSKIGFEL
                     MVCGENEEFARYCGISVRAYNIAAMAISGGLYGLSGALLVIGTQGRVMRGFSSGLGWS
                     AISVALLAQGSELAILPSALFIAFLKAGSDHVMIGSGVPSEIISILQAAAMLFITAQV
                     LPGLGQRRRQ"
     gene            2930..3817
                     /locus_tag="PLQ30_06460"
     CDS             2930..3817
                     /locus_tag="PLQ30_06460"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW02125.1"
                     /translation="MNLSLMALIDFLRYTIAAATPLLFASIGGLFSELSGMLNIALEG
                     LMAIGAFFGMAAAGLTNNIFLGALAAILSASLASMLMGLMTSKFRTNIFITGLAINML
                     AQGITAVLSQAWYHTKAVVAFDIPIDSHLFSSSISQLPLIGVLFSYRSMFTICAWLWV
                     LISAFIIKNTRHGLRIRATGSNPQAVSALGFSPDRIHMQALIWSGIGSGLGGYALGLS
                     ISAFVPNIISGRGWIALVAIYLGRKRPFWVAASCLLFAFAESSSNYLQGFSAIPPLLV
                     LALPYLVTLISLILAKREQ"
     gene            complement(3846..4514)
                     /locus_tag="PLQ30_06465"
     CDS             complement(3846..4514)
                     /locus_tag="PLQ30_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPW02126.1"
                     /translation="MIQNKHMICSLLFDLDNTLYSEATGLENGVAQRINLFIADFFGL
                     SISEAIRVRREHAKAYGTTLEWLMRDYGFQEPEEYFRAIHPEDEADCLNPDMVLKLIL
                     NNIPLPKAVLTNAPMDHAIRILDKLGISECFSGIYDIWFNKLVGKPHPGSYLGVLEAS
                     GFTLEHTLFIDDLPRYVKGYIDIGGLAVLKDEMDRFSDLPFRRLKTIYELDQILDQLN
                     SSYI"
     gene            4608..5336
                     /locus_tag="PLQ30_06470"
     CDS             4608..5336
                     /locus_tag="PLQ30_06470"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPW02127.1"
                     /translation="MIPIGDDNTCRRGSAIVNWILIALNILVFVFLQKFGLDDYTTLA
                     LAAIPEEILSGRRLFTLITSQFTHAGFAHLIGNMMFLGIFGDNVECRIGKLRYILLYL
                     ISGTIGMLLQIFLAAFAKGAALQMPLVGASAAISGVLATYLVLFPGNRVVVLLFYFIP
                     TVFSAWFVIGTWFFMQIIGGLSGLTAIQNGGTAYFAHIGGFLSAYIWGKSYKKKESKR
                     IAEWRKGPTAGYSDGFHWWIVDDD"
     gene            5340..6137
                     /gene="recO"
                     /locus_tag="PLQ30_06475"
     CDS             5340..6137
                     /gene="recO"
                     /locus_tag="PLQ30_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPW02128.1"
                     /translation="MAQRNERYRGLFLHTKDTARGDRILTFLCNEGLLSLFMFGGPKN
                     SLRSAALPFVLADIEVYRDSRRDFIKLTGANVLEAFDGIRKSFFHMQSASAASEFLIR
                     TSAFGGEYQQALLMITLLLRELARLDESETRLVLSAFFWDALYPLGLIPDLLRCERCG
                     RLFSEKNSLPGGLLIGESSFLCRSCAEEYGQIRFSTSVMLPFDDKILTCLHSIPKQSY
                     GQAVLLLKSIPSSPDIIKIIESLAEAAAEGPLQSLRILDQTFYRTQI"
     gene            6198..7508
                     /locus_tag="PLQ30_06480"
     CDS             6198..7508
                     /locus_tag="PLQ30_06480"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HPW02129.1"
                     /translation="MRAVDIIIKKRHGESLDEKEIRFFIEGYVNGEIPDYQVSALLMA
                     IFFQGMSAQETAALTQAMLDSGERINLCGIPGPFIDKHSTGGVGDKVSLPLAPMVAAC
                     GIKVPMMSGRALGHTGGTLDKLESIPGYMTSLEPAQFRKGLLEEGFAMTGQTAHIVPA
                     DKKLYALRDVTGTIESIPLITASILSKKVAEGAEGIVFDVKCGSGAFMKSYDDAKALA
                     KSLVATANTMGKRVVAVLTDMSQPLGYKVGNFLEIEETLDCLEGKGPKDVMELIYWLG
                     AWMLLLGGKAKDISECQNLCQEAIESKAALKLFYRNVVRQGGDLEEMQKRRGSWRSEF
                     SMEIRAEKDGYIVSIDALRIGLAGVYLGVGRNRTEDSVSPTAGFIFDKKSGDPVTKGE
                     RIATLFGKDSQSLEDAFPIALDAISIGDSAPSASSIILEEITVS"
     gene            7505..>7736
                     /locus_tag="PLQ30_06485"
     CDS             7505..>7736
                     /locus_tag="PLQ30_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPW02130.1"
                     /translation="MKWIKREIDPVQVRGLSQRYEVDTLTASILVRRNITDPEQIQFY
                     LENDLQLLHNPFLFNDMEDAVDRIITAREEEEK"
BASE COUNT         2093 a         1691 c         1768 g         2184 t
ORIGIN      
        1 tcagagttgc tcaagagact ggcaagtatc tcgtcttctt cgatgacgat gaatttgcac
       61 gggctcctga caatatcctt ggatgcactg ttctgcatca agaggagctt gcttaccgca
      121 gcttgaaggc agcaatcgaa gaaaaactgc aatttggaaa ggccgaaata gtaggcatga
      181 aagaaggcta catagaattc ttgaacaaga atccttctta tatcaaaaat gtaccagaat
      241 ctatccgcat aaaagtcgat gcggtaatcc aatcgatcaa atcgggtcgg cttacctttc
      301 aggttccttc actgtaatat gctcgctatg ctcgcggtaa ggatgcaagg aatatacagg
      361 cgctattacc ctagaggagt tcatgcaaat gatggcgcat ctctcgaagt cgaaaaaggt
      421 agtattcacg ccatcgttgg agagaatgga gcaggcaaga ctaccctaat gaaaatcctg
      481 gctggccttg atcaacccga tgaaggaaat atcgaaatag aaggaaaatt cgtaaagatc
      541 gaatcgccta gcaaggctca agcccttgga atcggcatgg ttcatcaaca tttcctgatg
      601 ttcgatgagc tcagtgccgc ggagaacatt ttttttggca tcgaaccaca tcgaaacaat
      661 aaggtttcga aaatagcagg cattgttgac tcgcaggagc taaatgcaag aaccctgcag
      721 cttgccacag aatatggctt tgctatcgat ccttcagcac cagctgcttg cttaagcatc
      781 gctgctcgtc agcagatcga aatactgcgg cagcttgcgc gccataccaa gatattgatt
      841 ctcgacgaac ccagttctgt tctcacagaa caagagacaa aggtactatt tgaaaaacta
      901 tccgaaataa agcgcctggg tcacacaatt attcttgtaa ctcacaagat ccatgaggca
      961 atgcagatag ccgatagggt aaccgtaatg cgcggaggaa agacagtcgg aacctatgac
     1021 atctccgaaa ccactcttga agagctcaca tcccttatca tgggaaagag ctttatcgag
     1081 gctacacaaa agcctataga aaagccacaa ataaaaaata ctcaaaaaaa acctatagtc
     1141 ttgcatattg atggattgaa cgtccgtcgc catcagcatt atggagtagg gctttcagat
     1201 atatctttcg aagtgcatgc cggcgaaatc ctgggcatca gcgcgctgag cggcaatggg
     1261 ctcgacgaac tcgaggacgt acttgctgga tttcgatctc catcttctgg aacttgtacc
     1321 ttcgatggtc ataatgtaga gcttcataat tctcgaaggt atcaaagctt atttcaaaaa
     1381 agacttattg cgtatctacc atcggatcgc attagaagag gaattgcacc ttctctttcc
     1441 gtacgagata attttattgc tctctccaga caggattttt ttcccaaagg ctggattcta
     1501 gaaaatgccg caaaacaagc gattgtaaat gcgattcatt ccttcgagat aaatgctctc
     1561 cccgatcagc ctgcaagaga gctctccggt ggcaatattc aaaaattgtc tattgcacgc
     1621 cttttctctc gagacttacc caggctcttt atcctatgcg aaccgacctg gagcctggat
     1681 attcgctcgg ccgagcatac ttacaggaga attcgcaatg ccagagaaag agggactgca
     1741 attcttctgc tatcctcgaa tatcgatgaa atactcgaat tgtcagatta tgtcatggta
     1801 ctctcccgag gcagacgagt tttgtatcaa gaaaacggca attggctcaa tagagccgtt
     1861 atcggtgaat accttcttgg caacaggatt catgaatgag aaatcaaggg gcatcttcta
     1921 ctgctcttaa ggttcttcta gcagcaactc tttccattat tcttgcgaca acagtactct
     1981 tgttgtttgc agcggatttt agatctgcat tatggcagtt cttcgtggaa cccttctcca
     2041 gccggtattt ttttggaaac tacattgccg atactgtgcc tctgataatt gcaggtcttg
     2101 gtgtatccat tgcttttaga tctagaaatt tcaatctggg gggagaaggc cagctttatg
     2161 caggagctct cgcgggagca gtactggcat tgcacatttc agaagcattt ggtccgagcg
     2221 cagtgtggat tctagtcgct tttatcgggg catttatagg agccatgatt ggcacaatct
     2281 cggggcttct aaaagcatat tggcaggtct ctgagatgat ttcatcctat cttctctcgg
     2341 caattatcat caatataagc gatttcttca ttaccggttt gcttcaagac aggacaagta
     2401 attttcagac tacggcccag attccatcgg aatttctttt gccaaggatc tttgcccctt
     2461 ccaaactaga tatctctata tggtttgctc tctttcttat cttgtttttc cagatcttat
     2521 ctaagaaatc caaaataggt ttcgaattga tggtttgcgg agaaaatgag gaatttgcac
     2581 gatattgtgg catttccgtt cgagcatata atattgcggc catggccatc tctggtggcc
     2641 tgtatgggct atcaggcgct cttcttgtta tcgggacaca aggtcgagtc atgcgcggct
     2701 tttcttccgg gctaggctgg agtgcaattt cagttgctct ccttgcacaa ggcagcgagc
     2761 ttgcaatact gccctcggct ctttttattg cctttttaaa agcaggctct gaccatgtaa
     2821 tgattggatc tggggttcct tcggagatta tttctattct ccaggctgct gcgatgctat
     2881 ttatcacagc ccaggtattg ccgggattag ggcagaggcg aagacaataa tgaacttatc
     2941 gctaatggcc ttgatagact tcttgagata tacaatagcc gcggcaacgc ccttgctttt
     3001 cgcttcaatc ggaggtcttt tttcggagct ttccggtatg ctaaatatag cgctagaagg
     3061 acttatggct atcggagcct tctttggcat ggcagccgcg ggtttgacaa ataacatctt
     3121 tttgggagct cttgccgcga tcctctccgc ttcgcttgcc tcgatgttga tgggcctaat
     3181 gactagtaag ttccgaacga atatctttat cacgggttta gcaatcaata tgcttgctca
     3241 gggtataaca gcagtcttgt ctcaagcctg gtaccacaca aaagcggtcg tcgcattcga
     3301 catcccaatc gatagtcatt tattctcaag ctcgatatct caacttcctt taattggcgt
     3361 cttattctct tatcgttcaa tgttcacgat ttgtgcgtgg ctatgggttt tgatttctgc
     3421 tttcataata aaaaatacaa ggcacggtct aagaattcgt gctacaggct ccaaccctca
     3481 agctgtctcc gctcttgggt tttctcctga tcggatacat atgcaggctc tcatctggtc
     3541 cggcataggt agcggattag gcggatatgc cttgggctta tctatttctg ccttcgtgcc
     3601 aaacattatc tcggggcgcg gatggatcgc actggtggct atatatcttg gccgaaaaag
     3661 acctttttgg gttgctgcat cctgtttgct ctttgcattt gccgaaagct catcgaacta
     3721 tctgcaaggt ttctctgcaa taccgcctct gcttgtcctt gccttacctt atctggtcac
     3781 tcttatctcg cttatcctgg ctaagagaga acaatagcga aggttaatct atatataagg
     3841 ttaatctata tataagaaga attgagttgg tctaatattt gatctagttc ataaattgtc
     3901 tttaggcgcc taaagggcag gtctgaaaag cgatccattt catcttttaa aaccgctagc
     3961 cctcctatat cgatatagcc ttttacgtac cttggaagat catcgataaa aagtgtatgc
     4021 tccagcgtga atcccgaggc ttccaaaacg ccaagatagg aacctggatg tggtttgcca
     4081 acaagcttat tgaaccagat atcataaatt ccggagaagc actcgctaat acccaactta
     4141 tcaagaatcc tgatcgcatg atccatagga gcatttgtta gcacggcctt tggtaggggt
     4201 atattgttca ggatgagctt tagcaccata tccggattga gacaatccgc ttcatcctcc
     4261 ggatgaatag ccctaaagta ttcttcgggc tcctggaatc cataatctcg catgagccac
     4321 tcgagggtgg tgccatatgc ttttgcatgc tcacgtcgaa ccctgattgc ttcagaaata
     4381 gagagcccaa aaaaatcagc aataaaaaga tttattctct gtgcaacccc attttccagg
     4441 ccagttgctt cggaatacag agtattgtcg agatcgaata gcaaggagca gatcatgtgt
     4501 ttattttgta tcatgaattg cacattggcg acaagcgaag gaaaaaacta gtctataata
     4561 agacaatatg ttattctaaa tagagtggca tttgaagagg agcttctatg attccaattg
     4621 gagacgataa tacttgtcgt cgcggcagtg cgatcgtaaa ctggattctc attgctctaa
     4681 atatattggt ttttgttttc ctgcaaaaat ttggcttaga cgactataca actctggctc
     4741 ttgcggcgat tccagaagag attttgtctg gaagacgatt gttcactcta attacaagtc
     4801 agttcactca tgcggggttt gcacatttga tcggaaatat gatgtttctt ggaatattcg
     4861 gtgataatgt cgaatgtcga ataggcaaac ttcgatatat tctgctttat ttgatctccg
     4921 gaacaatagg aatgctcttg cagatattcc ttgctgcttt tgcaaaaggc gccgcgcttc
     4981 aaatgcctct tgtaggtgcc tctgctgcga tctcaggcgt actcgctaca tatctcgttt
     5041 tgtttcctgg caatagagtt gtcgtacttc tattttactt cataccaacg gtattttctg
     5101 cttggtttgt cataggaacc tggtttttta tgcaaattat cggagggttg agcggcctta
     5161 cggccattca gaatgggggc acagcttatt ttgcacatat aggaggattc ttaagcgcct
     5221 atatatgggg caaatcctat aaaaaaaagg aatcgaaacg cattgcagaa tggcgtaaag
     5281 gtcctactgc cggctactcc gatggcttcc attggtggat tgtggatgat gattgatcca
     5341 tggctcagag aaacgaaaga tatagaggcc tttttctcca tacaaaggac actgcccggg
     5401 gcgatagaat tctaaccttc ctatgtaatg aaggcctttt atcacttttt atgttcggag
     5461 gaccgaaaaa ctctctgcgt tcggccgctt tgcctttcgt attagctgat attgaagtat
     5521 atcgtgattc gcggcgcgat tttataaaac tgactggcgc aaatgttctg gaagctttcg
     5581 atggtattcg taagagcttt tttcatatgc aatcggcgag cgcggcatcg gagttcttga
     5641 ttcgaacctc cgcgtttggc ggtgaatacc aacaagctct attgatgatt accctactcc
     5701 ttcgtgagct cgccagatta gacgaatcag aaactcgatt ggtcctgtcg gctttctttt
     5761 gggatgctct ttatccgctt ggcctcattc ctgatctttt gagatgtgag agatgtggca
     5821 ggcttttttc agagaagaat tcccttccag gaggactttt gattggagaa agctcctttc
     5881 tgtgtcgtag ctgtgcagaa gagtacgggc agataagatt ttcaacctca gtaatgcttc
     5941 ctttcgacga taagattctc acatgtctcc atagcattcc aaagcaatcc tatggccaag
     6001 ccgtacttct attgaaatca attccttcga gccctgacat aatcaaaata atcgagtcac
     6061 ttgccgaagc cgcagcagaa ggccctctgc agagccttcg aattcttgat cagacctttt
     6121 atagaactca aatttgaagt aaaatggttt tcaacattcg tttatcaata gagaagcaga
     6181 tttaggagaa aatcttgatg agggctgtag atatcataat aaaaaagcgc catggcgaat
     6241 cgctcgatga aaaagagatt cgctttttta tcgaaggcta tgtgaatggc gagataccgg
     6301 attatcaggt ttccgctcta ttgatggcga tattctttca aggaatgtct gcacaggaga
     6361 cagctgctct tacccaggct atgctagatt cgggagagag aataaatctc tgcggaattc
     6421 caggcccttt catagacaag cattctacgg gtggagttgg cgataaggta tcgctaccgc
     6481 tcgctccaat ggttgcagct tgcggaataa aagtgccgat gatgtcaggc agagcccttg
     6541 gccatacagg agggactctt gacaagctgg aatccatccc gggctatatg actagccttg
     6601 aacccgcgca atttcgaaaa ggcctgctag aagaaggctt cgcaatgaca ggtcagactg
     6661 cgcatatcgt tccagccgac aagaagctat atgcgctaag ggatgtaacc ggaacaatcg
     6721 agtcgatccc tctcataact gcttcgatat tatcaaagaa agtagcagaa ggcgcagaag
     6781 gtatcgtatt cgatgtaaaa tgcggctccg gggcatttat gaaaagctat gatgatgcca
     6841 aagctctggc caaaagtctc gtcgcaaccg ctaacactat gggaaaacga gtcgtcgcag
     6901 tattgactga tatgtctcaa cccctgggat acaaagtggg caattttctt gagattgaag
     6961 agacactgga ctgtctcgaa gggaagggac ctaaggacgt aatggagctt atatattggc
     7021 tgggagcgtg gatgctcctc cttggaggca aagcaaagga tatttcggaa tgccagaatc
     7081 tatgccaaga agcaatagaa tcaaaggccg cactcaagct tttctatcga aatgtggtaa
     7141 ggcaaggtgg cgatctggaa gagatgcaaa aaagaagagg atcgtggcgc tccgaattct
     7201 ctatggaaat tagggctgaa aaggatggat atatcgtttc cattgatgct ctcagaatcg
     7261 gactcgcagg tgtatacctt ggtgtaggaa ggaatcgaac agaagacagt gtcagcccca
     7321 cggcgggatt tatctttgat aaaaagtccg gcgacccagt gacaaagggt gaacgtattg
     7381 cgacgctatt tgggaaagac agccaaagcc tggaagacgc ttttcctatc gctctggatg
     7441 ccatttccat aggagacagc gctccatctg catcctcaat tattcttgaa gaaattaccg
     7501 tatcatgaag tggataaaaa gagaaataga tccagtacaa gtgcgaggtc tttctcagcg
     7561 ctacgaagta gatactttga cagcctcaat tctcgtgcgc agaaacatta ccgatccaga
     7621 gcaaattcag ttctatctcg agaacgatct tcagctactc cataatcctt tcctatttaa
     7681 tgacatggag gatgcggtag accgaattat cacagcccga gaagaagaag aaaaag
//
LOCUS       DAOMZL010000075         7560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_9190_length_7560_cov_16.457695, whole genome shotgun sequence.
ACCESSION   DAOMZL010000075 DAOMZL010000000
VERSION     DAOMZL010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7560
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9190_length_7560_cov_16.457695"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1179)
                     /locus_tag="PLQ30_06490"
     CDS             complement(37..1179)
                     /locus_tag="PLQ30_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPW02131.1"
                     /translation="MKKLHYGMVGGGKGAFIGNIHRQAIALDNLARLTAGSFSHDFSN
                     TLEIGMDLEIPSDRLYPTYLEMAQKEAARKDRIDFVSIVTPNYLHYPIAKAFLNAGIN
                     VVCDKPLALRESEARELDELTIKNGLLFAVTYAYTGYPLVKQAREMVKRGDLGDIRFI
                     NAEYPQDWLLKQFESDGHKLKSWRANPSVAGISNCVGDIGTHIEYLVSYITGLKIKSL
                     CARLDTFMEGHELDDNATIMLEFEGGAKGVYWTSQTAIGYDNGLKIRIFGSKGSLRWS
                     QEDPNYLVVSKLNSPTEIISRGRDDLYPKAQDYSRIPAGHPEGYIEAFANIYRAFTNA
                     LLKKSLGDIVTEEELDFPNARDGANGVRFVEKCVESSKKGAIWVEF"
     gene            complement(1192..2229)
                     /locus_tag="PLQ30_06495"
     CDS             complement(1192..2229)
                     /locus_tag="PLQ30_06495"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPW02132.1"
                     /translation="MRTNKRIRIGVIGVGKMGEQHIKNISYLCPGAILVALHDANQDR
                     AKEIAALYGFPQVFDDPDELIASPIVDAVLITAPDNVHADLILACLREKKPVLCEKPL
                     ASNLKDAKLVLDTEAELNQLLVSLGFQRRFDPAHLAVKEAAMEGVIGRPILWKGIHRN
                     SHAPYATNPFFLLTNTAGHDIDSARWLLGSEVKEVYARALRSRDDFPPDSRDLFLLEM
                     IMNDERLASAEIYMSADYGYEVSAELVGQNGVLTTARPDILIVRAQSKRYAPIAQDWT
                     SPFQEAYAAELNAWIESLAAGRIFPGANTWDGYVAIAVAEAANKSLIKGTSYPVELPR
                     KPGLYRLDSID"
     gene            complement(2248..3267)
                     /locus_tag="PLQ30_06500"
     CDS             complement(2248..3267)
                     /locus_tag="PLQ30_06500"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPW02133.1"
                     /translation="MSSILRIGIVGLGRLGKRHAENLSRKVQGASLVAAASPVDLERE
                     WAEKNFGIATYESLDELLDHSDAEIIWLVTPTSLHADQCIQVLEAGKHVFCEKPLALD
                     VESCDRVIEIAREHPKQRIMIGFMRRFDPAYSQVKREIENYGLGRIFRIHSESHDPID
                     PDGFFLKFAPTSGGIFLDCAIHDIDLTRWMLGGLEPKRVTAYGTRVMYPGLESCNDVD
                     TAVATVEFEKDVLATFHVSRTSHKGYQAIMTVTGTEGAINCGCGLSELPLLREKHGET
                     RIQGQTDFFSRFSDAFLYEANAFVASISSGVSVGATLEDAREATRLAIAMRESLEKGT
                     SIKLD"
     gene            complement(3388..4341)
                     /locus_tag="PLQ30_06505"
     CDS             complement(3388..4341)
                     /locus_tag="PLQ30_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPW02134.1"
                     /translation="MKKMKRFVSIILMVAFVFAISAPAFAADKRLKVGVVLKTLSSQY
                     WKIVAAGAKTAAQNNNVDLILLGPPTEDAIEQQINMVQDVLSQNIDVLVFSPSQPAAS
                     VNVLLQAKAKKIPVILVDTGMPEGFNDYASFIGTDNLAAGKAGGKALASQLKPGSKVL
                     LIDGAPGNPACNDRIKGAEEVLKASGMIIASKQPAYSDREKGYTVTQNVLQANPDING
                     VFSANDEMALGALRAMQQAGKRVPIIGVDATEDALKSILAGEMYGSVAQGNYDMGLLA
                     IEKAIELKAGKTIEKRIDSGATLITKENAQDLLDFRMSIKD"
     gene            complement(4449..5441)
                     /locus_tag="PLQ30_06510"
     CDS             complement(4449..5441)
                     /locus_tag="PLQ30_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW02135.1"
                     /translation="MSQVKLSSLTIKQSKTRGSLYNLGVLLSLVLLIIIMSILSPRFL
                     TISNLSNVASQASVSAIVSVGMFLAILTSGIDLSVGSVLALSTMVMGIVSVKLNVNPY
                     LSILICLGTGALLGLINGLLLTKLHLPHPFISTLGMQKMARGIALVITAASPISMFPA
                     PIQFLGNNYIGRLPISIVLVVMLYICVHLFLTRTATGRYIYAVGGNKEASRLSGIKVD
                     SVLNLVYMMSGFTAALAGIVLVGRVNAAYPLAGLDYETDAIAAVIIGGASFFGGIGSI
                     GNTIVGVLIIAVLRNGLNLLNVDSNYQTFAIGAVIIIAVAIDVLRQRVMSGSKK"
     gene            complement(5468..6979)
                     /locus_tag="PLQ30_06515"
     CDS             complement(5468..6979)
                     /locus_tag="PLQ30_06515"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPW02136.1"
                     /translation="MSDNSLLVELTGITKRFPGVLALDSVDMNIVSGEVHGLVGENGA
                     GKSTLIKILTGAYTHDEGKIIFDGKQLTQLTPSKAIELGIACIYQELDLIPHMTVVEN
                     IFLGRELYKTKGLKIIDRKRMLEETESLLKNLKLNFDPTKNTCDLGIGHRQMVEICKA
                     VNSNAKLIIMDEPTSSLSEHETEELFEIIAQLKEKGVTVVYISHRLDEIKRICDRITV
                     LRDGKKVADLNINDASIDNIIHLMVGRDIVNKYPKIKSNRGKEALSVQGLERKGILHN
                     ISFKAYTGEILGITGLVGAGRTEMARAIIGADPIDAGVVKIFGEEMNIRSPRDSIKAA
                     IAFLPEDRRNQGLVLIKDVLFNVSLVKLKQYSHRGFLQLKNIHQDVDQLVSELKIRTP
                     SVNTIVAELSGGNQQKVVIAKWLLTKARIFIFDEPTRGIDVGAKVEVYNLINELIKND
                     AAIIIISSEMDEVMEMADRIIVMHEGEIMGEFAHEEATQEKILYLASGIKISA"
     gene            7311..>7560
                     /locus_tag="PLQ30_06520"
     CDS             7311..>7560
                     /locus_tag="PLQ30_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPW02137.1"
                     /translation="MGSVEGSIMTDDLCSQYSITCGAEAEKLRNSIMDIAGVSEIFKA
                     LSDETRTKILYLISKRELCVCDLAFLLDMTLPAVSHHLR"
BASE COUNT         2075 a         1737 c         1493 g         2255 t
ORIGIN      
        1 attgatcata atcattttcc atttttctcc cttcggtcag aactcaaccc aaattgctcc
       61 ctttttggaa ctctcgacac atttttctac aaaacgtact ccattggctc cgtctcttgc
      121 atttggaaaa tcaagttcct cctcggtaac aatatctcca aggctttttt tcaacagagc
      181 attagtgaat gctctataaa tattcgcaaa ggcttcgata taaccctcag gatggccagc
      241 tggaattcgc gaataatctt gcgcttttgg gtaaagatca tctcgacctc gagagataat
      301 ttctgtgggg ctatttagtt tagaaacaac aagataattt ggatcctctt gtgaccacct
      361 taatgatcct tttgaaccaa aaattcttat tttcaatcca ttatcatagc caatagcagt
      421 ttggctagtc caatatacgc ccttagcgcc tccctcaaac tcaagcatga tagttgcatt
      481 gtcatccagt tcatgacctt ccataaaggt gtcaagtcgc gcacagagag atttgatttt
      541 taggcctgtt atatagctaa caaggtattc tatatgtgtg ccaatgtctc caacacaatt
      601 ggatattcca gctacggaag gattggctcg ccatgattta agcttatgtc catcggattc
      661 gaattgcttt agcagccaat cttggggata ttcggcattt ataaatctga tatctccaag
      721 gtcgcctcgt ttgaccattt ccctagcttg ttttaccaaa ggatagcctg tatatgcata
      781 ggtaactgca aaaagcaacc cgttttttat tgttaattca tcaagctctc ttgcttcgga
      841 ttctcttaat gccaaaggct tatcacaaac cacattaatt ccagcattga ggaatgcttt
      901 tgcaatagga tagtgaaggt aatttggtgt gactattgaa acaaagtcta tacgatcctt
      961 acgcgcggct tctttttgag ccatctccag atatgtgggg tatagtcgat cgctgggaat
     1021 ttctaagtcc attcctattt caagcgtatt gctaaaatca tgcgaaaaac tccccgctgt
     1081 aagtcttgca agattgtcca atgcaatagc ttgtctatgt atatttccaa tgaatgcgcc
     1141 ttttccgccg cccaccattc cataatgcaa ttttttcatg cttttactcc atcagtcgat
     1201 agaatctagt cgatagagcc cgggcttcct gggaagctcg acaggataag aagttccttt
     1261 gataagggat ttattggcag cttccgcaac cgcaattgct acatatccat cccaggtgtt
     1321 cgcaccagga aagatacgac cagctgcaag agattcaatc catgcattta gttcggcagc
     1381 gtaagcttcc tggaaaggag aagtccagtc ttgtgcaatc ggcgcgtatc gcttggattg
     1441 tgcgcgaact ataagaatat ctggccgtgc ggtggtgagc acgccattct gccctacaag
     1501 ctcagcagaa acttcatagc cataatcagc agacatatag atctctgcag aagccaggcg
     1561 ctcatcattc ataatcattt caagaaggaa aaggtcacgc gaatcgggcg gaaaatcatc
     1621 gcgggagcgc agagctctgg cataaacttc tttgacttcg ctgcccaata gccatctcgc
     1681 cgaatcgata tcatgacctg cagtattcgt gagaagaaag aagggatttg tagcatacgg
     1741 agcgtgtgaa ttcctatgaa ttcctttcca taaaataggt ctcccaataa caccctccat
     1801 tgccgcttct ttaactgcta agtgagcagg atcaaagcgt cgctggaagc caagggagac
     1861 aagaagctga tttaattccg cttcggtatc taggaccaat tttgcatctt tcagattgga
     1921 agccaggggc ttttcgcaaa gaacaggctt cttttctcgt aaacaagcga gtatgagatc
     1981 cgcatggacg ttgtcgggtg cagtaataag aacggcatcc actattggcg atgcaattag
     2041 ttcatctgga tcatcaaaga cttgaggaaa tccatagagg gctgcaatct ccttggcccg
     2101 atcttgattg gcatcatgga gagcgacaag aatagctccg gggcagagat aggaaatatt
     2161 cttgatatgc tgttctccca ttttgccaac tccgatgaca ccgatcctaa ttctcttatt
     2221 ggtcctcatg ttgtttcccc tttttattca atcgagcttt atggatgttc ccttttccaa
     2281 tgattctcgc atcgcaatgg ccaggcgagt tgcctctcgt gcatcctcaa gtgtggcacc
     2341 gactgatact cctgatgaaa tagaagctac aaatgcattt gcttcatata aaaaggcatc
     2401 gctgaatcgc gaaaagaaat ctgtttggcc ttggattctt gtctctccat gcttttcgcg
     2461 taacagaggg agctcgctaa ggccacaacc gcaatttatt gctccttcgg ttcctgttac
     2521 cgtcattatt gcttggtatc ctttatgcga tgtacgagaa acatgaaatg tcgctaatac
     2581 atctttttca aattcgacag tagcgaccgc ggtatcgaca tcattacagc tttcgagccc
     2641 cggatacatt actcgtgttc catatgcggt aacacgcttt ggctcaagac ctccgagcat
     2701 ccagcgagtg agatctatat catggatagc gcaatcaaga aaaataccac cagaagtagg
     2761 tgcaaatttc aagaaaaaac catcaggatc tattgggtca tgggattcac tatgaattct
     2821 gaatattctt ccaagtccat agttctcaat ttctcttttg acctggctgt acgcaggatc
     2881 aaatcgacgc atgaatccta tcatgattct ctgttttggg tgctctcttg ctatttcaat
     2941 aacccgatcg caggattcaa catcgagtgc caatggtttc tcacaaaaaa catgctttcc
     3001 agcttcaaga acttgaatgc attgatcggc atgcaaagaa gttggggtaa caagccagat
     3061 tatttcggca tcgctatggt ctaagagctc atctagactt tcataagttg caatgccaaa
     3121 attcttttca gcccattccc gttccaaatc aacagggctt gcggcagcga caagggaagc
     3181 tccctggact ttcctagaga gattttcagc atgtcgtttg ccaagccgcc ctaatccaac
     3241 aataccaatc cgcaggatac tcgacactat tacctccgta caaaatgtca tgcaaattgc
     3301 cgatatctcg aaacctatta ggaacacctg caagaagaca catcctcctg caggtgtttg
     3361 acctatccgt atatagagat attactacta gtctttaatg gacatacgga aatccaatag
     3421 gtcctgtgca ttttctttgg ttatcagcgt agcaccggaa tcgatacgct tttcaatcgt
     3481 cttacccgct ttaagctcta tagctttttc aattgcgagc agccccatat cgtaattccc
     3541 ctgggctacg gaaccataca tttctcccgc aagaatagat ttcagagcat cttctgtagc
     3601 atcgacacca ataatcggga cacgtttgcc agcttgctgc attgcacgaa gggcaccgag
     3661 agccatttca tcattagcag aaaacactcc attgatgtcg ggattggctt gcaaaacatt
     3721 ttgggttacc gtataaccct tctctcgatc gctgtatgct ggctgtttgg aagcgataat
     3781 cataccagag gccttgagca cttcctcagc ccctttgata cgatcgttgc atgcgggatt
     3841 gccgggagcc ccatctataa gcaggacttt gctgcctggt tttagttgag atgcaagggc
     3901 ttttccacca gcttttccag cggccaagtt gtcagtaccg atgaaacttg cataatcatt
     3961 gaagccttct ggcatacctg tgtcgacaag gataacaggg attttcttag cctttgcctg
     4021 caatagcaca ttgacggatg cggcaggctg agatggagaa aatactaaga catcaatatt
     4081 ctgcgataga acatcctgaa ccatattaat ctgttgttca atggcatctt cggttggtgg
     4141 tccaagaaga atgagatcca cattattatt ctgagcagcc gtcttggcgc cggcagcaac
     4201 gattttccag tattggctgc tgagcgtttt aagtacgacg cccactttca gtcgtttgtc
     4261 agcggcgaat gctggtgcac ttattgcaaa gacaaatgct accatcagaa taatcgaaac
     4321 aaaccttttc atctttttca tctctgaaac ctcctaaata aaatttgagt tcgagcgact
     4381 ttcttgttta ttctttctcg tttatcacct ccttttcgtt gcatttagat taggatttgc
     4441 ttagatgact atttcttgct gccgctcata actcgttgtc gtagaacgtc aattgcaaca
     4501 gcaataatta tgaccgcacc aatggcaaat gtttggtaat ttgagtcgac attgagcaaa
     4561 ttgagaccat ttctcagcac cgcaattatc aggacgccaa cgattgtatt tccgatggat
     4621 ccaataccgc caaagaaact tgcccctcca attattacag cggcgattgc atctgtttca
     4681 taatcgagac ctgccaatgg ataagcggcg ttgactcgtc caactaagac tatccccgcc
     4741 agtgcagcgg tgaagccgct catcatatag accaggttaa gaacagaatc caccttgata
     4801 ccactcaatc tgctggcttc cttattaccg cctacggcat atatatagcg accagttgct
     4861 gttcttgtaa gaaaaagatg tacacatata tagagcatca caaccagaac aatgctgatt
     4921 ggaagtcttc ctatataatt gttacccaag aattgtattg gcgccggaaa catgctgatt
     4981 ggcgatgctg cggtgataac aagcgctatg cccctggcca tcttctgcat accaagagtc
     5041 gatatgaagg gatgaggcag atgcagtttt gtaagaagca gtccattgat cagtcccaat
     5101 agagctcccg ttcccagaca tatcaagatc gaaagatagg gatttacgtt caatttaacg
     5161 ctgacaattc ccattaccat tgttgaaaga gcaagaacag agccaaccga aagatctatg
     5221 ccggatgtaa gaattgccaa aaacatgcct acagacacaa tggcactaac agaagcttga
     5281 gaagccacat tggatagatt actaattgtc aagaacctgg gtgataatat agacatgatt
     5341 atgattagca aaaccagact cagaagaacc cctaaattat agaggctacc ccttgtttta
     5401 ctttgtttta ttgtgagtga actcaattta acttgggaca tagagctgct ccttcaatag
     5461 agtaaattta tgctgatatt ttgattccag atgccagata tagaattttt tcctgggttg
     5521 cttcctcgtg ggcaaattcc cccattattt ctccttcatg cattacaata atgcggtctg
     5581 ccatttccat aacttcatcc atttccgagc ttataataat tatggcggca tcatttttta
     5641 ttagctcatt tatcaaattg tagacttcca ctttggcccc gacatcaatg cctcgtgtgg
     5701 gttcatcaaa tataaagatc ctagccttgg ttagaagcca ttttgcaatg actacttttt
     5761 gctgatttcc tccactgagt tcagctacaa ttgtattgac gctgggcgtg cgaattttca
     5821 gctcagaaac tagctgatct acatcttgat gaatattctt taattgcaaa aatcctctgt
     5881 gcgagtattg ctttaatttt acgagagata cattgaataa tacatctttt atgagaacaa
     5941 ggccttgatt cctgcggtct tctggcaaga aagcaatggc agcctttatt gaatcccttg
     6001 gagaccgaat attcatttcc tctccaaata tctttacaac cccggcatcg attgggtctg
     6061 cgccaatgat agcccgagcc atctctgtac gcccagctcc tactaatcca gtaatgccca
     6121 ggatttcgcc tgtataagcc ttaaaggaaa tattgtgcaa tatacccttt ctctcgagac
     6181 cttgaaccga aagggcttct ttaccacgat tgctctttat ttttggatat ttattgacaa
     6241 tatcccggcc aaccattaga tgaatgatat tatcgattga agcatcgttt atattcaaat
     6301 ctgcaacttt cttgccatcg cgcagaacgg ttatgcgatc acatatgcgc tttatctcat
     6361 ccagtcgatg cgatatataa acaacagtta ctcctttttc ctttagttga gcaatgatct
     6421 cgaagagttc ttcagtttca tgctcactta agctcgacgt gggttcatcc atgattatta
     6481 gttttgcatt ggagttgacg gccttacaaa tttcaaccat ttggcgatgt ccgatgccaa
     6541 gatcacaggt attctttgtt ggatcgaaat tcagtttgag attcttaagc agagattctg
     6601 tctcttctag catgcgcttg cggtcaataa tctttaagcc ctttgtctta tataattcac
     6661 gaccaaggaa aatattctct acgacggtca tgtgcggtat aagatcaagt tcctggtata
     6721 tgcaagctat tcccagttct attgctttag atggagtcaa ttgtgtcaac tgtttcccat
     6781 cgaatattat ctttccttca tcatgcgtgt atgctccagt aagtatcttt atcaatgttg
     6841 atttaccagc tccattctca cctaccaggc catggacttc gccagacaca atgttcatat
     6901 caacagaatc aagagccaag accccaggga atcgcttggt aattccagtg agttcaacca
     6961 ataagctatt gtcgctcata aaatctccta ttctaggcta ttcagtctca aaacctattg
     7021 tataagcaac aacataagat cttagcctat tcgagattca tcgatatgaa aaccttttct
     7081 gaaaaggttt tcatatcggc taaagcttat tattgtacca ctttgccaat ttgtcaaata
     7141 attatgtaaa tcatcacgtc aaaacgattc tgccatatga aaggtgcaca ttatccttgc
     7201 gaatgaggct ctgaaaaaaa taatctctct atatcttgat caagtctgtt atatgcatta
     7261 caatatactt aaacagttgc ttaagtgagc aatcgtaaat tcagttttca atgggatctg
     7321 ttgagggttc gattatgacc gatgatctat gcagccaata cagcataacc tgtggggcgg
     7381 aggctgaaaa acttcgaaat tcgatcatgg atatcgcagg agtttcggag atattcaagg
     7441 cgctatcgga tgaaacgcgg actaagattc tttatctcat ttcgaagcgc gaattgtgtg
     7501 tctgcgatct cgcttttctc ttagatatga ccttgccagc ggtctcgcat catcttcgat
//
LOCUS       DAOMZL010000076         7455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_9324_length_7455_cov_14.968243, whole genome shotgun sequence.
ACCESSION   DAOMZL010000076 DAOMZL010000000
VERSION     DAOMZL010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9324_length_7455_cov_14.968243"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PLQ30_06525"
     CDS             complement(<1..1004)
                     /locus_tag="PLQ30_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPW02138.1"
                     /translation="MDNKKYSRRRSALAAELMRKGLSAVIVSDFENGRDSSLRYLCGQ
                     PSDALLVVNKNATSVLIAWDVNMAHRMGDSNHILPFTDFGRQSAQALKAALDLLGLEK
                     GSKIALPASTPYPEYIELVQSLDAFDLICEKNGIDRFIKEMRSRKDAEEIILYQEICQ
                     YTDTLMDMVEKEVAAGTLSTELDVALFLERQARRMGAEGMGFESLVAGPERSFGIHAF
                     PTFTAGPFANKGLSILDFGLRFEGYTSDVTMSFVDNILSQEQKTMIDLVEEAHRIAVE
                     ACGPEVSLLKVATLVDEFFAKAGWFMPHSLGHGIGLDAHELPTLSMRAMPDDLLLPGH
                     "
     gene            1334..2788
                     /gene="mnmE"
                     /locus_tag="PLQ30_06530"
     CDS             1334..2788
                     /gene="mnmE"
                     /locus_tag="PLQ30_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPW02139.1"
                     /translation="MSRSYFDSHVPIVALATPEGRSALAIIRTSGEAAIDIVARCFSQ
                     PEALLGAAGYEAVHGWIIDPVTKERIDEVMALVYKAPHSFTGENAVEILCHGSIVVVR
                     RILDVLYAQGFSPALQGEFSFRAFIEGKTDLVRAEAINELSLASCEAARHDALLRLSG
                     VLSRRLKELRSMMLDALADIDARLDYSEDEGPEKIESWPTIICQAQSSIIELLKSYPG
                     GKLRQEGLLVVIAGRPNAGKSSLFNLLVQEERAIVSSEPGTTRDWLESWITMGGLAVR
                     LVDTAGLRNSKNEIEIEGVRRARAMLDQADLILYLVDGTVGLYEEDKTFMASYPGALF
                     LWNKVDRTDCLAVPSGWKATSAKDLASFGSLESMLVDELRAKVDEGYGMQGHEPQGQN
                     PPTCNSQDPTPLSRERQSYERQIRIVSERQKQLLYKAQEALNRAMEAHKAGVALDLVS
                     LDIQEAAESIGEISGDIASDEVLEHIFSSFCLGK"
     gene            3010..4890
                     /gene="mnmG"
                     /locus_tag="PLQ30_06535"
     CDS             3010..4890
                     /gene="mnmG"
                     /locus_tag="PLQ30_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPW02140.1"
                     /translation="MGDFEAIVIGGGHAGVEAALALARLGTKTVLITQNPDTIARMSC
                     NPAIGGLSKGNLVREIDALGGQMGILADATAIQTRMLNRSRGAAVQAPRAQADKALYA
                     RLARDALESQPNLSIFMDTVVDFILSKDVHPRIEGVVTERGNTIGARVVVLTTGTFME
                     AKLFIGAWSGPGGRLGEPAAMGLGTALREKGFPLGRMKTGTPARIKRESIDFSKLDVQ
                     HGDKAKIFFSFLEHDYERPDVPCYVIYTNEATHEAIRSGLDRSPLFSGSIVGKGPRYC
                     PSIEDKVMRFPERERHQAFIEPEGLFTDEMYLNGLSSSLPEDVQERFYHSILGLENAI
                     IVRPAYAVEYDYIDPAALYASLESKLVEGLFIAGQTNGTSGYEEAAAQGLMAGINARR
                     KLDGSSPIILGRNEAYIGVLIDDLVTLSPKEPYRMFTSRAEYRLSLRHDTADLRLTPY
                     AIEMKLVDSRRRENFEHRVRTIEEVKALLEGHKINHEDIAQLPELEGHLGQALSDAIR
                     DPKVGSLYDDEIIALSRLAKILPQTASMQASGVITALLNERYKGYQEKENRLASRLAH
                     AEKLLIPRGFDYKAVKGLSQEASEKLSASQPLTLGQASRIPGVRKSDVALLYIALSKR
                     PR"
     gene            complement(5000..5641)
                     /locus_tag="PLQ30_06540"
     CDS             complement(5000..5641)
                     /locus_tag="PLQ30_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02141.1"
                     /translation="MAVIKSALELALERTKDLQLDESAQKLADARIEGRKAASQYLED
                     QTSVDFKAILNKLEPVQRQTFLTAAFEVLINFIQLPTNSVVETEKMESIGKAIVLLCG
                     LSPRFSSEREVKLAQQQARSLYQQILRFLSQYQEEMKRVEQAIRNQWAPKLRERERQL
                     AAALGQNVRIDPMSDPEFAEFYRKNIESMRNNYGKALEDAKKQLADTCGFESK"
     gene            complement(5677..6471)
                     /locus_tag="PLQ30_06545"
     CDS             complement(5677..6471)
                     /locus_tag="PLQ30_06545"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPW02142.1"
                     /translation="MEEKDRIADLPRNILLTLSIFFIVQCIIFLTFSLIVGFESQYWT
                     IFLPISTGFHILILIMLLLFRDDFYIESTGQKLDRINLANIITLSRVSSMPTLLVLVI
                     AAKEYSIRIPVLILIALIFLTDFFDGLISRKTNQVTKIGKILDSVSDYALLIVLSIIF
                     QYYLLIPGWLFWLIITRFGIQALFMAVLMIIAKRIEPKSTFMGKVTIASIMVLYTLEI
                     IKMILQASSHMVFRIAEWIVAIIIVIGIFDKIASFFDTIKSKPSAP"
     gene            6611..7243
                     /gene="pgsA"
                     /locus_tag="PLQ30_06550"
     CDS             6611..7243
                     /gene="pgsA"
                     /locus_tag="PLQ30_06550"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPW02143.1"
                     /translation="MTIADKITLSRMIMAPFFFFFVRAEFIPRQPAIIILWILFAWME
                     ISDLIDGKVARRSNQVGSFGKLFDPFADVISRVTYFLAFASIGIMPLWVLLIVMYREF
                     GILFLRALLALKGTAMGARPGGKLKAGTYMVAGLLSLCLYTVQTYASSMNSAIIDGFV
                     NHSDKMTEIASVLHSLTSISYVVVALATILSFIDYMIQFKKIYGQTNEKV"
BASE COUNT         1994 a         1672 c         1787 g         2002 t
ORIGIN      
        1 atatgcccag gcagcaagag atcatctggc atggctcgca tgcttagcgt aggtaattca
       61 tgcgcatcta aaccaatccc atgaccaagc gaatgtggca tgaaccatcc agctttggca
      121 aagaattcat caacaagtgt agcaaccttc aagagagata cttctggtcc acatgcctcc
      181 accgcaatgc gatgcgcctc ctctacaaga tcgatcatgg tcttttgctc ctgactaaga
      241 atattgtcta caaagctcat ggtcacatcg ctggtgtagc cctcgaagcg cagaccaaaa
      301 tcgagaatgg aaagcccttt gttagcaaaa ggacccgcgg taaaggtagg aaaagcgtgg
      361 attccaaaag agcgctccgg tccagcgaca aggctttcga atcccattcc ctccgcaccc
      421 attcgccttg cttgtcgctc tagaaaaaga gcgacatcca gctctgtaga aagagtacca
      481 gctgcaactt ccttctccac catatccatc agggtgtcgg tgtattggca tatttcttga
      541 tatagaatta tttcttctgc atcctttctt gatcgcattt cctttataaa cctatcgatg
      601 ccgttttttt cgcatataag gtcgaatgca tcgagagatt gaacgagttc tatatattcg
      661 ggatagggag tagaagcagg aagcgcaatc ttcgagccct tctctaaacc caagagatcg
      721 agcgccgcct taagcgcttg agcggattgt ctcccaaaat ccgtgaatgg tagaatatga
      781 ttcgaatccc ccattcgatg agccatatta acatcccagg cgataagcac agatgtagca
      841 ttcttattga ccaccagcaa tgcatccgaa ggttgaccgc acagatagcg caacgacgaa
      901 tcgcgcccat tctcaaaatc cgatactatc acggcgctta ggccttttcg cataagctct
      961 gccgcgagcg cagagcgcct tcttgagtat ttcttgttgt ccatgcatgc tccttaggat
     1021 tcaaagtaaa ccgacaataa cagagcaatt atcctccgac gcaaaggctc tgtccagtga
     1081 gggttgacta aattacaagc gagctgaatc agagttgtaa aagcctggag ccacatacta
     1141 gattcccttt ggagctgcct tattatggag acattttgga ttagggcaga aagggaaaga
     1201 ctgaatagtc agttccttgc tattccctta ttcggccttt ttcctccttc ttatttcaac
     1261 ttcttataca caaggttcgt agcagtatag actccttgta ataataggcg ctgaaaggtt
     1321 atcgtagttc tgtatgtctc gaagctattt tgatagtcat gtgcccatag tggcgctggc
     1381 cactccagag ggccgatctg cgcttgccat catccgaact tcaggcgagg ctgcaataga
     1441 catcgtagct cgctgctttt cgcagcctga ggctcttctt ggcgccgcag gctatgaggc
     1501 agttcatggt tggataatcg atcctgtcac taaagagcgc atcgatgagg ttatggcgct
     1561 ggtctataaa gcaccgcatt catttacagg agagaatgcg gtggaaatac tatgccatgg
     1621 ctcgattgta gtggttcgtc ggattctgga tgttctatat gcccaggggt tctctcctgc
     1681 tttgcagggc gagttttctt ttcgtgcctt tattgaaggc aaaacagatc tagttcgtgc
     1741 tgaggccata aatgaattaa gcctggcttc ttgtgaagcg gctcggcacg atgctctttt
     1801 gcggctttcc ggagtgcttt ctaggcgact aaaagagcta cgcagcatga tgttggatgc
     1861 gctagcggat attgatgcac ggctcgatta ctcagaagac gaaggaccag aaaagatcga
     1921 aagctggccc acgattatat gtcaggctca aagttctatc attgagcttc ttaaaagtta
     1981 tcctggcgga aaattgcggc aagaaggtct attggtggta atcgctggaa gacctaatgc
     2041 tggcaaatcg agtctattta acctgcttgt gcaagaagaa cgagccatag tgagttcaga
     2101 gccaggtacg acccgcgatt ggctcgaatc ctggataacg atgggaggct tagcagtgag
     2161 gcttgtggac acagctggtc ttcggaactc aaagaatgag atagaaattg aaggtgtgcg
     2221 tcgagccagg gctatgctgg atcaggccga tctgattctc tatctggtag atgggactgt
     2281 tggcctctat gaagaagaca aaacctttat ggcctcatat cctggggctc tctttctctg
     2341 gaacaaagtc gatcggaccg attgcttggc agtgccttcg ggctggaagg cgacaagtgc
     2401 caaggatctc gcttcctttg ggtctttgga atcgatgctg gttgatgaac ttcgagcaaa
     2461 ggtcgatgaa ggctatggca tgcaaggcca cgagccgcaa ggccagaacc ccccgacctg
     2521 taattcccaa gatccaactc cgctaagccg ggagcgacaa agctatgaga gacagatcag
     2581 gatcgtaagc gagaggcaaa agcaacttct ttataaagcg caggaggctc taaatcgcgc
     2641 tatggaagcc cataaggctg gggtggcgct cgatcttgtt tctctggata ttcaagaagc
     2701 ggctgagtcc ataggagaga tttcaggcga catagcttcg gacgaggtgc tggagcatat
     2761 attcagcagt ttttgcctgg gcaagtgaac aagccgcgct aaacgcaaaa gaggaattga
     2821 gagcgagaga agaaggcgag cgcgcaggga gcgccgatgc gagagcaaag agttcgcgct
     2881 tgcacaagat agccaatgaa gccctggcgc aagatagcgc atggcaagac ctcaaaatgt
     2941 agatttaaga tcgcaaaggg gcaaaagtgc gaaatcgcgc gaacgattag acgattagga
     3001 gagattggaa tgggagattt cgaggctatt gtcataggag gggggcatgc tggcgtcgaa
     3061 gccgccctag cccttgcaag gcttggaacc aagaccgtac tcattacgca gaatcccgac
     3121 actatagcca gaatgtcctg taatcctgcc ataggtggcc tttctaaagg taacctcgtg
     3181 cgcgaaatcg atgcgctcgg gggacagatg ggcattttag cagacgcaac cgccatccag
     3241 acgcgcatgc tcaatcgctc ccggggtgcg gcggtccaag caccaagggc acaagccgac
     3301 aaagcactct atgcgagact agccagagat gctctcgaga gccagccgaa tctttcaatt
     3361 tttatggata cggttgtgga ctttatactg tctaaagatg ttcatcctcg catcgaagga
     3421 gtggtaactg agcgcggaaa taccattgga gctcgggttg tagttctgac tacaggcact
     3481 tttatggaag caaagctctt tatcggtgca tggagtgggc ctggcggccg ccttggagaa
     3541 ccagcggcaa tggggttggg aacagccctc cgcgaaaaag gatttccttt agggcgtatg
     3601 aagacaggaa caccggcacg gataaagcga gaaagcatcg atttttctaa gctcgatgtg
     3661 cagcacggag acaaggccaa gatattcttt tcctttcttg agcacgatta tgagcgaccc
     3721 gatgttccat gctatgtcat atacaccaat gaggcgaccc atgaggcaat tcgctctgga
     3781 ttagatcgct ctccgctttt ttcgggcagc attgtcggaa aagggccaag atattgccct
     3841 tcgatcgaag acaaagtaat gagatttccg gaaagagagc ggcatcaggc ttttatagag
     3901 ccagaaggcc tttttacgga tgagatgtat ctaaatgggc tttctagttc gctaccggaa
     3961 gatgttcagg agcgcttcta tcacagcatt ttaggccttg aaaatgcgat aattgttaga
     4021 cccgcctatg ccgtcgaata cgattatatc gatccagctg cgctctatgc ctccttagag
     4081 tcgaagcttg tagaagggct ttttatagca ggccagacca atggaacctc gggatatgaa
     4141 gaagctgctg cccaaggcct catggcagga atcaatgctc gccggaaact cgatggttca
     4201 tcaccgatta tccttggaag aaacgaggcc tatattggtg ttctaatcga tgatcttgtt
     4261 acgctttcgc caaaggaacc ttatcgcatg tttacgagta gagctgaata ccgactttct
     4321 ctccgccacg atactgcgga tctgcgactc accccctatg caattgaaat gaagcttgtt
     4381 gattcgcgtc gtcgcgagaa tttcgagcat agagtaagaa ccatagaaga agtaaaagcc
     4441 cttctcgaag gtcataaaat caatcatgag gatatagcgc agttgcctga acttgaaggc
     4501 caccttggtc aagcgctttc cgatgcgatt cgcgatccaa aagtcggttc tctatacgac
     4561 gacgaaatta tagcgctttc gcgcctggca aaaattttgc ctcagaccgc cagtatgcaa
     4621 gcctcgggcg tcataacagc acttctcaat gaacgctaca agggatacca agaaaaggaa
     4681 aatcgtctcg caagccgtct tgctcacgca gagaagcttt taattccacg gggttttgat
     4741 tacaaagcag tcaaagggct atctcaagaa gcctcagaga agctaagcgc aagccagccc
     4801 ctcacgctcg gtcaagcttc gagaattccg ggagtccgga aatcggatgt tgcgcttctg
     4861 tatatagcac tcagcaagcg tccccgctag ttaatctgat attgcgcttc tatgtatagc
     4921 tattgcctcg agatggcctt tgattgaaat atcctattgc cgacaaagat gatttatgat
     4981 ccagattgcc ctgtcttgat tatttggact cgaaaccgca ggtatctgca agctgctttt
     5041 ttgcatcttc cagggctttt ccatagttat tccgcattga ttcgatattc ttccgataga
     5101 attccgcaaa ctcggggtcg gacatcggat ctattcttac attttggcct aatgctgcgg
     5161 ctagttgtcg ctctctctct ctaagcttgg gcgcccactg attccgaatt gcttgttcga
     5221 ctcgcttcat ttcctcttga tattgactca aaaaacgaag tatttgctga taaagggagc
     5281 gtgcttgctg ctgcgcaagc tttacttctc gttctgatga aaagcgtgga gaaagaccgc
     5341 atagcaagac tatagcctta cctatggatt ccattttctc ggtctcgact accgagttgg
     5401 ttggcagctg gataaagtta atgagcactt cgaaggctgc ggtcaaaaaa gtctggcgct
     5461 gaacaggttc caatttgttc aatattgcct tgaaatcgac tgaagtctga tcttccaaat
     5521 actggcttgc cgcttttcga ccctcgatcc tcgcatcggc taatttttgg gcactttcgt
     5581 cgagctgcag gtcttttgtc ctctcgagtg cgagctctaa ggcacttttt atgacagcca
     5641 ttccaattcc tcctcgatta ttgagaataa tataaatcag ggtgcggagg gtttgctctt
     5701 gattgtatca aaaaagctgg caattttatc gaaaatacca ataacaatta taatggcaac
     5761 tatccactcc gctatccgga ataccatatg ggaagaagct tgaaggatca tctttatgat
     5821 ttcaagcgta taaagcacca ttatagatgc aatggtcacc ttgcccataa atgtggattt
     5881 tggctcgatg cgttttgcga ttatcatgag caccgccata aacagcgcct gaattccaaa
     5941 gcgcgttata atgagccaga agagccatcc cggaataagt aaataatact gaaaaataat
     6001 actcaggaca ataagcaacg cataatcgct aaccgagtcc agtattttgc ctatttttgt
     6061 aacctgattg gtttttctgg atataagccc atcgaaaaag tctgtaagaa aaataagagc
     6121 aatcaatata aggaccggta ttcgtatact atattctttt gcagctatta ccagcaccaa
     6181 aagagtaggc atggaactta ctctgctaag cgtaattata ttggcaagat ttattcgatc
     6241 gagtttttga cctgtggatt cgatatagaa gtcgtcccga aaaagaagca acattatgag
     6301 aataagaata tgaaatccag tgctaatcgg caggaagata gtccaatatt gcgattcgaa
     6361 tcccacaatt aaagaaaagg tcaggaagat tatgcattga acgatgaaaa atatcgacaa
     6421 tgtgagcagg atgtttcgag gaaggtcagc aattctatct ttttcttcca tgaatatttt
     6481 tatcctatag gatagccaca tttattttgc ttgatatgca aaggcgtaca atggccgaaa
     6541 tttacttatt atactatagc ctcgattata atcttgacaa gcttgatgac taatccgtat
     6601 gcttcctttg atgaccatag ccgataagat aacgcttagc cgaatgatta tggcgccttt
     6661 ttttttcttt tttgttagag ccgaatttat cccgcgtcag cccgctatta tcatactatg
     6721 gatactattt gcctggatgg aaatcagcga tctcatcgat ggaaaggtag ccagaagaag
     6781 caatcaggta ggatcttttg gcaagctatt cgatcctttt gcggatgtaa tctctcgtgt
     6841 tacatatttt ttggcttttg ctagcatagg cataatgcct ctttgggtgc ttctgatagt
     6901 gatgtatcgc gaattcggca ttctctttct gcgagcactg cttgcgctta aaggaaccgc
     6961 catgggagcc cgtcctggtg gcaagttgaa agcgggtacc tatatggtgg ctggcttgct
     7021 ttcgttgtgc ttgtatactg ttcagaccta tgcttctagc atgaatagtg cgattattga
     7081 cggttttgtg aatcattctg acaaaatgac agagattgcg agtgttttac attctttgac
     7141 ctctatatcc tatgttgttg tagcacttgc cactattttg tcttttatag actatatgat
     7201 ccagttcaag aagatttatg ggcaaacgaa cgaaaaagta tagagttttt tttgaataat
     7261 tgccaaggaa cctcttgacc aactctagct tgttcggtta tgttctcatc cgcgcccatc
     7321 acttagtgct gcatgttatg taataatttg caataataag caaataaatg cgaaatatgc
     7381 aaaggctttt gggttttgca agcacttgac aactatcgat agatttggta gtttcatcga
     7441 gcgctcttta ggtta
//
LOCUS       DAOMZL010000077         7411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_9394_length_7411_cov_15.146683, whole genome shotgun sequence.
ACCESSION   DAOMZL010000077 DAOMZL010000000
VERSION     DAOMZL010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9394_length_7411_cov_15.146683"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..2049
                     /locus_tag="PLQ30_06555"
     CDS             313..2049
                     /locus_tag="PLQ30_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02144.1"
                     /translation="MKYLKIRDLTLRDGQQSQFATRMNNEQVKAVLDDYRQSHFYAME
                     VWGGAVPDSVMRFLGEDPWDRLAIIKEGIGNASKLTALSRGRNLFGYNPYPDSVIEGF
                     CKNAIAAGVDIMRIFDALNDIDNMKSSIRFVKEAGGLADCAVCYTVDPHFRLSDRIKG
                     VFTGKKIPPKIFTVDYYVKKAKELASLGADIFTIKDMAGLITPFMAAELVKALKNELD
                     IPIDLHTHCTPGFGVASLLAAMVNGVDIVDTAILSFSGGPAAPAYEIIRIFADRLGFS
                     VDVDNAAVARIDKRLRQIRSELASFDQYKRLPPELDLSKDSLSPELSKLFDDALEAAV
                     GGKYQKSLDICQQIEKAFFYPEPDEIVRHAQIPGGMYTNMLTQLKEAKLEHLLNEVLR
                     LVPTVRLDAGLPPLVTPTSQIVGVQAVNCVISIHEGKDMYENVSKNFEDLILGQYGKT
                     PWPVDPEFRYKICGTREEVPYDTSKYKPQDNPPIPEAGGVPLALNEKEQLLLELFPSV
                     AAKFLRNKRIAEWQAAHPEGTPSKEQPGLGEQNLAPYFDPDYEPSDGEPPSFWEIAPS
                     WAGWTDSIALGQ"
     gene            complement(2079..3512)
                     /locus_tag="PLQ30_06560"
     CDS             complement(2079..3512)
                     /locus_tag="PLQ30_06560"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPW02145.1"
                     /translation="MEIERIDPFSYIIPVREGMRVPARIYASESLFSLLHEDKSIEQL
                     VNVARQPGIVKEALGMPDMHQGFAFPIGGVAAFDIDEGIVSPGGVGFDINCGVRVILT
                     SLDEKIVRPKLESIGKKLFARVPSGVGSKGVLKVSAKEARKAMDNGLRWTLAKGFAEN
                     IDLESVESGGYLEEADERAVSERAIDRGKEEFGTLGAGNHFLEISTVERIFDPARAKA
                     YGLFEGQVLIWIHTGSRGLGHQVATDYIARMRPRMTHYKIPLYEHDFVSIPISDPIAK
                     EYLSAMAAAANFAWINRQMITVAVREVFAQEFGEPWDSLGMRLLYDVAHNIAKFETHI
                     VEGMEKKLLVHRKGATRAFPAGHPELSLHAREVGQPVLLPGDMMRGSYILVGGPRAME
                     ESFGSVAHGAGRCMSRKAAARQIQFDALSDQMRAHSVILLAANKRLACEEAPLAYKNV
                     NDVVETVSGAGLASLVARTKPLLVIKG"
     gene            complement(3527..4381)
                     /gene="murI"
                     /locus_tag="PLQ30_06565"
     CDS             complement(3527..4381)
                     /gene="murI"
                     /locus_tag="PLQ30_06565"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPW02146.1"
                     /translation="MNEKIGVIDSGMGGLTVVRSLFLRNVPADIVYVGDNANVPYGNR
                     PLDEIVELTLRMLKVLSEHNIRIVAIACNTISATIERLKPYWAYPIIDIISPVAQFLA
                     NCGESDIGLFATEFTVRSGLHAKLVGEINPGVHIHGVPSRRLAALIDESIEDESAIRE
                     EISSMLAQLAALHPVHKVLLGCTHYPIVMDLFEKLAPEKEYIDPADLQAKAIIDLLNK
                     TELKSRSIENGREGVSTEIEKQAKGVLFSETAKPSLEIVTSGTEDAYRTMLSRLGIPK
                     ARSIDRLL"
     gene            complement(4478..5209)
                     /locus_tag="PLQ30_06570"
     CDS             complement(4478..5209)
                     /locus_tag="PLQ30_06570"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPW02147.1"
                     /translation="MGLFQKKKHFLAFIPCIFGLVIWSLIPIAAGAQSEPGHSIQVYG
                     QASMYVEPDVANLLIGIEIQADSAAKAQEQLRYQASVVFDAISQASIQQPKMKTSSYR
                     ISPLYSNEKGKEHIIEGYKAETSIAVEISDLSSVAIIIDKAIEAGANIVRSVYYDRRD
                     LEAFKAQLIKSACIEASSKAEIAASALGFKLGAPIRVSVQDRFSAPDTGNMIMKASAF
                     GSESLRPGELEISVTVSAEFELMSD"
     gene            complement(5329..5934)
                     /locus_tag="PLQ30_06575"
     CDS             complement(5329..5934)
                     /locus_tag="PLQ30_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPW02148.1"
                     /translation="MGRERLFADKKEIIDAAIELLSENGYESFSTRKLARRLGVSPMT
                     LYNYFSSRNEIIEAAIEAAYERVFEEFYNDLSSYLEKESFCPLCGFMEIGKKLLLFSR
                     EKPKIYALVFIMNIMPYREQPSVQRLFGITLSKVRARLTAEEISQDLQNHIYLFMLLV
                     LALVQNVYNGNSSYDIRAFEANLRIAYNQLLKPFEKYFSFA"
     gene            complement(6064..7140)
                     /locus_tag="PLQ30_06580"
     CDS             complement(6064..7140)
                     /locus_tag="PLQ30_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HPW02149.1"
                     /translation="MTTIVLSGGGGTRLWPLSRQSIPKQYCNLLDGLTLFERTIKRNI
                     KLSDNIIVVTNVAHFDIAKSQFRKVCPDKQVHFILEPCGRNTAPGIALACLELDPEEN
                     VLVVPSDHIILDEEAYQDAVNRALVAANLGYLVTFGLKPEYAETGFGYIEADGDKETA
                     GEGFLLVKAFKEKPDRATAEEYIAAGRYYWNSGIFSFLAGKYLYELGNTAPEILLASK
                     KAWEAARKSISQNTEDVIAIPEEAMLSIPAESIDYAVMEKSQNVAVVPCSLGWSDLGS
                     WDAIWKIRERSGLEALEGSSTISIESKRNLVVGSERIVALVDVEDLIVVDTPDALLIS
                     KRGSSQNVKRIVEQLKEKGKTHLL"
BASE COUNT         1990 a         1767 c         1585 g         2069 t
ORIGIN      
        1 gttgcaacct ttgcagtatt ttactctgac gatgtgaaaa aatttctttt gaggaccagc
       61 catttctttg ctcttgaaaa ataatcgccg cactcaatct aacagaccga gcattgctcc
      121 tttagatgtt ccttcaaaaa agaaaaaatt taaaggataa atataagcag gaacgcatta
      181 cggcgttttt ttattttaca gtttattcac gattttgtca aaatctatca cttgtgccat
      241 aaatcatata tattgccgat acttgtcgct tatggcgatt tgagatacaa atagacttca
      301 ggagcttttg agatgaaata cttaaagatt cgcgatttga cacttcgcga tggccaacag
      361 tcacaatttg ccacacgcat gaataatgag caggtcaagg ccgtgctaga tgattatcgc
      421 cagagtcatt tttatgcaat ggaggtatgg ggcggagccg ttcctgattc tgtaatgaga
      481 ttcctcggtg aagatccatg ggataggctc gctataatca aagaaggaat aggcaatgcc
      541 tcaaaattga cagcactctc acgtgggcgc aatctctttg gatataatcc ctatcctgat
      601 tcggtaatcg aagggttttg caagaatgcc attgcggcgg gtgtagatat catgagaatc
      661 ttcgatgcgc tcaacgatat agacaatatg aagtcttcga taaggtttgt aaaagaagcg
      721 ggtggtctgg ctgattgcgc tgtttgctac accgtcgatc cgcattttag attgagcgac
      781 agaataaaag gcgtttttac tggaaagaaa ataccgccta agattttcac agtagattat
      841 tatgtcaaga aagcaaagga gctggcatct ctcggcgctg acatttttac aatcaaagat
      901 atggcgggac tgattactcc tttcatggct gcagagcttg taaaagcgct caaaaatgag
      961 cttgatattc cgatcgatct tcatactcat tgtactccag gctttggagt ggcaagtctg
     1021 cttgcagcca tggtcaatgg agtcgacatt gtcgatacgg ccatcctgtc tttctctgga
     1081 gggcctgctg ctccagctta tgagataata cgcatttttg cagacaggtt gggcttctcc
     1141 gtggatgtcg acaatgctgc tgtcgcaagg atagacaagc gtcttagaca aatccgatcc
     1201 gaacttgcgt ccttcgatca gtacaagaga ctgccgcccg aactcgatct ttccaaggat
     1261 agcctatcgc ccgagctctc caagctcttc gatgacgctc ttgaggctgc cgttggaggc
     1321 aagtatcaaa aatctctcga catttgccag caaatagaaa aggccttctt ttaccctgaa
     1381 cccgatgaga tcgtacgtca cgcgcagata ccaggcggca tgtataccaa catgctcact
     1441 cagctcaagg aagccaagct ggaacactta ctcaatgaag ttcttcgtct tgtgccaact
     1501 gtgcggctcg atgcaggcct gcccccgctt gtaacaccta ccagtcagat tgtgggtgtc
     1561 caggcagtca actgcgtcat aagtattcac gagggaaagg acatgtacga aaacgtatcg
     1621 aagaatttcg aagaccttat cctcggtcaa tatggaaaga cgccatggcc agtcgatcct
     1681 gaattccgct acaagatctg tggcacaaga gaagaagttc cttatgacac aagcaagtac
     1741 aagcctcagg acaatccacc gattccggag gcaggtggag ttccgctggc attgaatgaa
     1801 aaagaacaac tgcttctaga gctattcccc tcggtggcgg caaagttcct gaggaacaaa
     1861 aggattgctg aatggcaagc ggcgcatcca gaaggcaccc cttctaaaga acaacccggg
     1921 cttggggaac agaatctcgc tccatacttc gatcctgatt atgagccaag cgatggagag
     1981 cctccatcct tctgggaaat tgcaccgagc tgggcaggat ggacggattc tatcgcctta
     2041 gggcagtaga aagcgcctta tgctctcctt atgatcttct agccctttat aaccaagagg
     2101 ggcttggtac gtgccacaag gcttgcaaga ccagcgccgc taactgtttc cacaacatcg
     2161 ttgacatttt tataggcaag aggagcttct tcgcaagcca atcgtttatt tgctgcaagc
     2221 aatattacgg aatgcgctcg catttggtct gaaagcgcat cgaactggat ctgacgcgcg
     2281 gcagccttgc ggctcataca ccgacctgcc ccgtgtgcaa cgcttccaaa gctctcttcc
     2341 atggctctag gcccgccaac caatatatag ctgcctctca tcatatcacc aggcaaaaga
     2401 acaggctggc caacttctcg cgcatgtagg gatagctctg ggtgaccagc aggaaaagca
     2461 cgcgtagcgc cctttctgtg aacaagaagc ttcttttcca tcccttcgac gatatgagtc
     2521 tcgaattttg ctatgttatg agcaacatca tataatagtc gcataccaag cgaatcccag
     2581 ggctctccaa attcctgggc aaaaacctca cgcaccgcga cggttatcat ctgcctgttt
     2641 atccatgcaa agtttgcagc cgctgccata gccgaaagat attccttggc aataggatcg
     2701 ctaataggaa tgctgacaaa atcatgttca tagagaggta tcttataatg tgtcattctc
     2761 gggcgcatgc gcgcgatgta atctgtggcg acctgatgtc ctaaacccct tgatccggtg
     2821 tggatccaga tgagcacctg cccctcgaat aggccatagg ccttagcgcg tgctgggtcg
     2881 aaaatccgtt ccaccgtgga tatctcgaga aaatggttgc ccgcccccag agttccaaat
     2941 tcttcctttc ctctatctat agctctttcg cttactgctc gctcatctgc ttcctccaga
     3001 tatcctccgc tttcaacgga ttcgaggtcg atattctccg caaacccctt agccaaagtc
     3061 cagcgcagcc cgttgtccat cgccttgcga gcttctttag cagatacctt caacacccct
     3121 tttgaaccaa ctcctgaagg cacgcgtgcg aaaagttttt tgccaatgga ttcaagtttc
     3181 ggacgtacaa ttttctcatc gagagaagtg aggattactc ttacaccgca atttatgtcg
     3241 aatcccacac cgccaggcga tacaatgcct tcatctatgt cgaatgcggc aacgcctcct
     3301 atcggaaaag caaaaccttg atgcatatcc ggcatgccaa gagcttcttt tactattccg
     3361 ggctgtcgag ctacatttac tagttgctct atggatttat cttcatgaag aaggctgaaa
     3421 agcgattcag aagcgtagat gcgtgcagga acacgcattc cttccctaac ggggattata
     3481 taggagaacg gatctatccg ttcgatttcc atatgcccct ctgcttctat aataaacgat
     3541 ctatactgcg ggcctttgga attcctaatc tgctcaacat agtacgatat gcatcctctg
     3601 taccagaagt gacgatttcc aaagaaggtt ttgcggtttc gctaaaaagg actcctttgg
     3661 cttgtttttc aatttcagtg cttacacctt ctcttccatt ttctatagat ctagatttca
     3721 gctcggtctt attcaatagg tcgattatgg ctttggcttg tagatccgca ggatcgatat
     3781 actccttctc tggtgccagc ttctcgaaca agtccataac aataggataa tgagtacagc
     3841 caagaagtac cttatgaact ggatgaagcg cggcaagctg agcaagcatg gatgatatct
     3901 cctcacggat agctgattca tcctctatcg attcatcgat aagggcagct aatctgcggc
     3961 ttggtactcc atgaatgtgt acgccaggat taatttcacc taccaatttt gcgtgcaagc
     4021 ctgatctgac ggtgaactcc gtggcaaata agcctatatc cgattctcca caattagcca
     4081 gaaattgcgc tactggagaa attatatcga ttatcggata tgcccaataa ggcttcaggc
     4141 gctctattgt cgcggaaata gtgttgcatg cgattgctac gattctaata ttatgttcgg
     4201 aaaggacttt cagcatgcgc agagtgagct caactatttc atccaatggc ctattgccat
     4261 aaggcacatt tgcattatca ccgacataga caatatcggc tggcacattc cttaaaaata
     4321 ggctccgaac aaccgtgagc ccgcccatgc cactatcgat gactccaatt ttctcgttca
     4381 tgaatacatt gatacatgac tattgttttc tgtcaatcga atgccataga gcaaaaatct
     4441 cttccttata taatagctca attatcgcac tgccttgcta atccgacatg agttcaaatt
     4501 cagcagacac tgtaacgctg atctcgagtt ctcctggcct tagagactct gagccgaagg
     4561 ctgacgcctt cataatcata ttcccagtat cgggggcgga aaacctatcc tgtacgctga
     4621 ctcttatggg agctcccaat ttaaagccta gcgcgctggc ggcaatctcg gcctttgaac
     4681 tagcctctat gcaggctgac tttatgagtt gagccttaaa tgcctccaaa tccctacgat
     4741 catagtagac tgaccgtaca atattcgccc cggcttctat tgccttgtcg atgataattg
     4801 ccacagatga cagatccgaa atctccaccg caatgcttgt ctccgcctta tagccttcta
     4861 tgatatgctc tttgcccttt tcattgctat ataaaggcga tatacggtaa gaactggtct
     4921 tcatcttggg ttgctggata cttgcttgag atatagcgtc gaaaaccact gatgcttgat
     4981 atcgaagttg ctcttgagcc tttgctgcgc tatcggcttg tatttcaatc ccgataagca
     5041 aattggccac atcaggctca acatacatcg acgcttgacc atacacctga atgctgtggc
     5101 ctggttccga ttgagcaccc gctgcaatgg gtattaaaga ccatatgaca agcccaaaga
     5161 tgcaaggaat aaaggcaaga aagtgttttt tcttttgaaa taagcccata gtctactctc
     5221 ctttgctatt ataagttgat ctagaggtct ttgatatagc tgttgctgct actatactgc
     5281 tacgaacctt gtgtatagga ataaagcaga ctttgtaccc tgctatgctt atgcaaatga
     5341 aaaatatttt tcgaaaggtt ttagaagctg attatacgca atccgaagat tggcttcgaa
     5401 agctcggata tcgtacgagc tattcccatt gtacacattc tgtaccagtg ctaagacaag
     5461 caacataaaa aggtagatat gattctgaag atcctggctg atttcttccg cagttaagcg
     5521 agctcgaacc tttgataaag taataccaaa aagtctttgt acactgggtt gttcgcgata
     5581 tggcatgata ttcattataa atacgagcgc atatatcttg ggcttttcac gagaaaacaa
     5641 tagtagtttc ttgcctattt ccataaagcc acagagaggg cagaaagact ctttttcaag
     5701 atatgatgaa agatcattat agaattcttc gaacactctc tcataggcag cttcgatggc
     5761 agcttcgatt atttcatttc tactagaaaa gtaattatat agcgtcatgg gagatacacc
     5821 aagtctcctg gcaagctttc gtgtagagaa actctcatat ccattctcgc ttagaagctc
     5881 tattgcggcg tcaataattt cttttttatc ggcaaaaaga cgttctcttc ccatattttt
     5941 attttatttt gaaggaagca agatgataag tcaatatata aaagattatt tattgtgtaa
     6001 gaaataattg ataacaaaac agatgcttag gtaattattc tttacatcga ttttgggatt
     6061 tttttacaag agatgagtct tgcctttctc ttttaactgc tcgacaattc tctttacatt
     6121 ctgactcgat ccgcgctttg aaatcagaag agcatcggga gtatcgacta caatcagatc
     6181 ctctacgtca acaagggcga caatacgttc agagcccaca acgaggtttc tctttgactc
     6241 aatggaaata gtcgacgatc cttctagagc ttctaagccg cttctctccc gaatcttcca
     6301 gatagcgtcc cagcttccaa ggtcgctcca gcccagcgaa caaggcacca ccgcaacatt
     6361 ctggcttttt tccatgactg catagtctat ggattctgcg ggtatcgaaa gcatcgcttc
     6421 ctcaggaatg gctattacat cttcagtatt ttgggaaatg gattttctag ctgcctccca
     6481 ggctttcttt gatgccaaga gtatctctgg cgccgtattg cccagctcgt aaaggtattt
     6541 gccagccaga aatgaaaata tcccggagtt ccaataatat cttcctgctg caatgtattc
     6601 ttcggcggta gctctgtcag gtttctcctt aaaggccttt acaagcagaa aaccttcgcc
     6661 cgcggtctct ttatctccat cagcttcgat gtagccgaaa cctgtttcag catattcagg
     6721 ctttagccca aaggttacaa ggtatccaag gtttgctgcc acaagagctc tattaaccgc
     6781 atcttgatag gcttcttcat ccaatatgat atgatcggat ggcacgacaa gcacgttctc
     6841 ctcaggatct agctcaagac aggccagtgc gatccccggc gctgtgttgc ggccacaagg
     6901 ttcaaggata aaatggacct gtttatcagg acagactttt ctaaactgac tttttgctat
     6961 atcgaaatgc gcgacattgg tgactactat gatgttatca gacaacttga tatttctctt
     7021 gattgtgcgc tcgaaaagcg taagtccgtc caaaagattg caatattgtt ttggaataga
     7081 ttgtctggaa agcggccata gcctagtacc accgcctccc gaaaggacga ttgttgtcat
     7141 tctcgatgat tttccccttc tttgattcga tgatcgagtg aatagactgt aatgcagaat
     7201 tttccaggat gcaatggcag atttcgttac tctgacaaga tgaggaaacc ataattcgcg
     7261 cgcattccca atgtccggtg gtttctgcat tcaggagcga taataagctt gtgcagacca
     7321 aactagattg cggcagtctt ttcgccgagc gtagtctgct tagaaaaata gtagcctccc
     7381 ataagagaga accgcacagc gcacagccat a
//
LOCUS       DAOMZL010000078         7337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_9533_length_7337_cov_15.180170, whole genome shotgun sequence.
ACCESSION   DAOMZL010000078 DAOMZL010000000
VERSION     DAOMZL010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7337
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9533_length_7337_cov_15.180170"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLQ30_06585"
     CDS             complement(<1..364)
                     /locus_tag="PLQ30_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02150.1"
                     /translation="MKKFYLIVLQRDRENLPERLRKLGVAHVEELQGIGDKYQNLERE
                     RMEVESVHNFLQNYIDKKSKPGKADSSKSLKYEECKDVREIINEISAAKQKLNLLEDQ
                     VIDLKREIDRVASWGEVSM"
     gene            complement(373..987)
                     /locus_tag="PLQ30_06590"
     CDS             complement(373..987)
                     /locus_tag="PLQ30_06590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPW02151.1"
                     /translation="MGKIKLTKNELKKKRDELKMYERYLPTLQLKKQQLQVEVRSVQT
                     RMEELQALKKTLEQDASPWMEVFAEQGAARTQEGEPLLKVAFISTDTGNIAGVAIPLF
                     LGVEFEHKGYDLYSTSLWVDAALILMERLVRTQLELEVVSKQKDLLSQELRITTQRVN
                     LFEKVKIPETIADIRKIRIYLGDQETAQVVRGKIAKRKVEGAER"
     gene            complement(1021..2319)
                     /locus_tag="PLQ30_06595"
     CDS             complement(1021..2319)
                     /locus_tag="PLQ30_06595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPW02152.1"
                     /translation="MNKIYSRIESIVGNVIAVKAQDIAYGELAQVNTRYGASLAETIR
                     LDSEMVYLQVFSGSRGISTGDEVRFLGHPMQVSFSDNLMGRIFDGAGEPRDGGPALTE
                     NLITIAGPAVNPYQRIIPREMIRTGIPMIDVFNTLVKSQKLPIFSVSGEPYNPLLARI
                     AMQAEVDLIILGGMGLKYDDYLFFKDTLEQGGSLSKTIMFVHTAADPIVECLMVPDIS
                     LAIAERFALKGKNVLVLLSDMTNFADALKEIAITQEQVPSNRGYPGDLYSQLAARYEK
                     AVDFSDAGSITILAVTTMPGDDVTHPIPDNTGYITEGQFYLRDGHIEPFGSLSRLKQL
                     VNAKTREDHRSIMDAMIKLFAAYRDTLEKKSMGFQMSAWDRKLLKYGELFEQRIMDLS
                     VNIPLEKALDLCWETLAECFEPRETGLRSDLIQKFWIGKK"
     gene            complement(2323..4084)
                     /locus_tag="PLQ30_06600"
                     /pseudo
     CDS             complement(2323..4084)
                     /locus_tag="PLQ30_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711822.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
     gene            complement(4081..4665)
                     /locus_tag="PLQ30_06605"
     CDS             complement(4081..4665)
                     /locus_tag="PLQ30_06605"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HPW02153.1"
                     /translation="MKHYWYFASTLTSFPFGSPPPISMQAFDELCSRMMDKSDLEILL
                     YVEEARNGRFSDSMMKSPFLMSYYEFERGLRNALVVYRTRATKQDAGQWTRPGDMNPD
                     SLKAAQAAQSASDPLQAELALERERWNATERLSALSAFELDYILAYKMKLLIASRIAS
                     FDRNRGMQGFNVLYQDIVNAAAEAASAFDTGVAT"
     gene            complement(4667..5284)
                     /locus_tag="PLQ30_06610"
     CDS             complement(4667..5284)
                     /locus_tag="PLQ30_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02154.1"
                     /translation="MEIQLQELLERIHSEGIENAKQQAEQIIQKAQSEAAEIIARAKR
                     DAEEMQAEALRRIDAMEAASRESLLQASRDTMIALRQSVQRFLDSAISADIKKAFDEE
                     MAAQVIPEVLKILSSGQSSDFEVLLPPEQLEKIDSSLSARLAKELSRGVTFKPYDGID
                     AGFRIALSGSSVQYDFSADSIAQMLSVRVNRLLSQYLKESAGSLD"
     gene            5637..5708
                     /locus_tag="PLQ30_06615"
     tRNA            5637..5708
                     /locus_tag="PLQ30_06615"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5670..5672,aa:Gly,seq:tcc)
     gene            5900..7141
                     /locus_tag="PLQ30_06620"
     CDS             5900..7141
                     /locus_tag="PLQ30_06620"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HPW02155.1"
                     /translation="MNSGITIMKPKVKTPIVELDGDEMTRVIWSMIKEKLILPYIDVH
                     LEYYDLGVANRDVTNDRVTMDAARAIAKYGVGVKCATITPDSERVKEYRLKKAWPSPN
                     ATIRAYLDGTVFRKPIIAKNIAPAVRSWQKPIIIGRHAYGDIYKAVDFVVPGPGTVSL
                     VYQPAQLGMPISFKVHEFEGPGVAMGIHNTDASIRSFARACIEYALSEKTDIWFAAKD
                     TISKNYHGHFKELFGEEISARSADLEAAGIHYRYMLIDDAVAQLMKNEGGILWACMNY
                     DGDVFSDMVASGFGSLGMMSSVLVSPSGAFEYEAAHGTVRRHYYEYLKGNPTSTNPIA
                     SIFAWTGALRKRAELDKTPEITIFAQQLEDTAIELIESGVVTKDLINLISPPAKSFET
                     TESFLDKIDAGLARKRTALQA"
BASE COUNT         1942 a         1771 c         1587 g         2037 t
ORIGIN      
        1 tcatcgagac ttctccccaa ctcgctactc tatctatttc tctttttaga tcaatgactt
       61 gatcctcaag caaattaagc ttttgcttcg cagcagatat ttcattgatg atttccctaa
      121 catctttgca ttcttcgtat ttgagagatt tcgaagaatc ggccttgcct ggtttggatt
      181 ttttatcgat atagttctga agaaaattat ggaccgattc aacctccatc ctttctcgct
      241 ctagattctg atatttatct ccgataccct gaagttcctc cacatgggct acaccgagtt
      301 ttcgaagtcg ctcgggaagg ttttctctat ctctctggag cacgatgagg tagaattttt
      361 tcataggcac tatcatcgtt cagctccttc cacctttctt tttgcgatct tgcccctaac
      421 tacttgagcc gtctcctgat cgcccaaata aatgcgaatc tttcgtatgt cggcgatggt
      481 ctcgggaatt ttcacttttt cgaaaaggtt tacgcgttga gttgttattc gtaactcctg
      541 cgaaagcagg tctttttgct ttgaaaccac ctctagctcc aattgtgtgc ggacaagccg
      601 ctccataagt atcaaagcag catcaaccca gagagaagtc gaatacagat catagccctt
      661 atgttcaaat tccactccaa ggaatagagg aatagcaact cccgcaatgt ttccggtatc
      721 ggtgctaata aacgcaacct tcaagagagg ctcaccttcc tgggtcctcg ctgcgccctg
      781 ctctgcgaaa acttccatcc atggagatgc atcttgctcg agtgtctttt taagggcttg
      841 gagctcctcc attcgagtct ggacgctgcg cacttcgact tgaagctgct gttttttaag
      901 ttgaagcgtt ggaagatagc gctcatacat cttgagctca tctcttttct ttttcaactc
      961 attcttggtc agcttgatct tccccatgat tctattgcct tctattcttg ctagctggaa
     1021 ttatttcttt cctatccaga acttttgtat gagatccgaa cgaagacctg tctcgcgcgg
     1081 ctcaaagcac tcagccaatg tctcccagca taggtccaag gccttctcca gaggtatgtt
     1141 gacagataaa tccatgatcc tctgctcaaa gagttctcca tatttgagga gcttacgatc
     1201 ccaggcgctc atctggaagc ccatcgattt cttctcgagg gtgtctctat atgcagcgaa
     1261 tagctttatc atcgcatcca tgatagagcg atgatcttcc ctggtcttgg cgttaaccaa
     1321 ttgctttagt cgcgagagag atccaaaagg ctcaatatgc ccatcgcgaa gatagaactg
     1381 gccttcggtt atatagccag tgttgtccgg aataggatgc gtcacgtcat ctccgggcat
     1441 tgtggtgaca gccagaatgg ttatggagcc tgcatcagaa aaatctacgg ctttttcgta
     1501 tcgtgcggct agttgtgaat acaaatctcc tggataaccg cggtttgatg gtacctgttc
     1561 ttgggtaata gcgatttcct tcaaagcatc agcaaagttt gtcatatcgc taagcaaaac
     1621 caggacattt ttgcctttga gagcaaaacg ctcggcaatt gccagagaaa tatccggcac
     1681 catgagacac tcgacgatag gatcggccgc tgtatgcacg aacattatgg ttttcgacaa
     1741 agaacccccc tgctccaggg tatctttgaa gaaaaggtag tcgtcatatt tgagccccat
     1801 acctcccagg atgataaggt cgacttcagc ctgcattgca atgcgcgcaa gaagaggatt
     1861 gtaaggctct cctgaaaccg aaaaaatagg aagcttctgg cttttgacaa gtgtgttgaa
     1921 cacatctatc attggaatac cggttcttat catctcgcgt ggaataatac gctggtaggg
     1981 gtttacagcc ggtccggcta tagttatgag attctccgta agagcaggac ctccatcgcg
     2041 cggctctcct gcaccatcaa agatgcgacc catgagattg tccgaaaagc taacttgcat
     2101 tgggtgccct aagaaccgca cctcgtcgcc tgtcgaaatg ccgcggctac ccgaaaaaac
     2161 ttgaagatac accatctcac tatccagacg gattgtctcc gccagagaag caccatatct
     2221 agtatttact tgcgctagct caccataagc aatatcctgc gcctttacag caataacgtt
     2281 acctacgatt gattcgattc tcgagtatat cttattcatg ccttactcca gcgtgctaat
     2341 caatttaagt ccacggtcat caaactcagg ttttctgtca accagagcct cctccaccct
     2401 attatataga gcgctaaaca tgtcgctatc ttgcggggta ccattcaagt ccagcatcgt
     2461 ttgacgaagc tcgtagaaga aattacgcgc ctcttcttta tcggagaatt ctataccagc
     2521 tgcaagtatc ttgcaccaac ttgaacacat gcttctgcct tgccggagat actgctgcat
     2581 cgacaggatc aaatgaattc tgctgcaagt ataccgcatc caagaattcg ctcttcagat
     2641 agattacata atcctccagg cttgtccctt cttcacctac aactttcatc atcgcgctta
     2701 tttcagctcc acgttccaag aatgaatgag catacctaac agcagatttg ggcataattc
     2761 catcatactt ggtccatgaa tcaagaggat ggatcgaagg atatttgcgc gcatccgagc
     2821 gctcacgcga aagcccatga aaagcaccta cgactttgag tgtcgcctgt gtcacaggtt
     2881 cttcaaagtt accgcctgcg ggcgaaaccg ttcctccgat agtaagggat ccctccttgc
     2941 cgttatatag ccttactata ccggcgcgtt cgtagaatgc agctataaca gactcaagat
     3001 atgccggaaa agcctcttct ccaggaatct cttcgagtcg tccggacatc tctcgcattg
     3061 cctgtgccca gcggcttgta gaatcggcaa gaagaagcac gtcgagaccc atttgtcggt
     3121 agtattccgc aatcgtcaca gccgtataga ccgaggcctc tcgagctgct acgggcatag
     3181 acgaagtgtt gcagataata acagtccgtt ccatcagaga gcggccagtc tttggatcga
     3241 tgatctgggg aaattccttg agtgtttcga caacttcgcc agcacgctcg ccacatgcgg
     3301 ctatcagcac aatgtcaacc tcggcatggc ggcttgttac ctgctgtaga actgtctttc
     3361 cagctccgaa aggtccgggg atacaataag tgccaccttt tgctactggg aaaaacgtat
     3421 ctatgattcg ggtttttgta acaagcggct ctgaaggctt aagacgctcg gaatagcaat
     3481 ccacggctct ctttacaggc caggaaaaac tcatttggat atgcctttta tttcctttca
     3541 aatccactac ttccgctatc gcctcatcta ccgtgtaatc gccttccgcc actatatctg
     3601 caactttcca ttctccatat agatcaaaag gcagcattat gatatgccga aaatatcgct
     3661 caggaaccca gcctataccc attccgcgtt tcagaatatc gccctttttc aagatcgggg
     3721 taaaatgcca ctttatatct cgaggaagag ccttgaggta taggcctggc tctaaaaaat
     3781 accctatctt gttagcaatc tccggcagag ggttttgtag tccatcgtat atctgcccga
     3841 gaagacccgg accaagctcg accgataata gctcattggt gaactctact ctgtttccga
     3901 cctttatacc tttggtaatt tcgtagacct gagcctgaac ggaagtaccg cgaatacgaa
     3961 ttacctcgct ctttagcctt ctttctccta ccaggatata agcgacctca ttcatggtta
     4021 caatcccttc agtttggatg gaaaccatat tgccatttac tgcggtgatg aatccttctg
     4081 tcatgttgct actccggtat caaaagcgct cgctgcttct gctgcagcat ttacgatatc
     4141 ttgatataag acattgaatc cttgcatccc gcgatttcta tcgaaggaag caattcgtga
     4201 tgcaataagc agtttcattt tatatgctag aatataatca agttcaaatg ccgaaagcgc
     4261 ggagagcctt tctgttgcgt tccatcgttc gcgctctaag gcaagctcgg cttggagcgg
     4321 atccgaagca gattgtgccg cttgcgctgc ttttagagaa tccggattca tatcccctgg
     4381 tctggtccat tggccagcat cttgctttgt agctcttgtt cgatagacta caagcgcatt
     4441 gcgcagccct ctttcaaatt cataatatga catcaaaaat ggagatttca tcatggagtc
     4501 agagaacctg ccattccttg cctcttctac atataagaga atttctaaat cacttttatc
     4561 catcatcctg gagcagagtt catcaaaagc ctgcatagag atgggagggg gtgagccaaa
     4621 tggaaaagat gtaagtgttg aagcaaagta ccaataatgc ttcatttcaa tcgaggctcc
     4681 ccgcagattc ttttaggtat tgagaaagga gcctgtttac acgaaccgaa agcatttgtg
     4741 caatgctgtc tgcagaaaag tcatattgca ccgaggaacc agacaaggct attctgaatc
     4801 cagcatcgat tccatcgtat ggcttgaatg tgacacctct tgagagttcc ttggcaagtc
     4861 ttgcagaaag agaactgtct attttctcga gttgctctgg tggcaaaagc acttcgaaat
     4921 cgctcgactg gccggatgag agaatcttca aaacttcagg gataacctgt gcggccattt
     4981 cctcatcgaa agctttcttg atatcggcgc tgattgctga atcgagaaag cgctgaacac
     5041 tttggcgcag agcaatcatt gtatccctag atgcttggag aagactttcg cgagaagctg
     5101 cttccattgc atcgatacgc ctcaaggcct cagcttgcat ctcctcagcg tctctcttag
     5161 cgcgcgctat aatttctgct gcttccgatt gagctttttg gataatctgt tctgcttgct
     5221 gttttgcatt ttcgatgcct tcgctatgaa ttctctcaag aagttcttgt aattggatct
     5281 ccatattaat tccctctgca taaaatcgac aacggaagaa aaggatcgta tctcatcaat
     5341 gcactaatat ctacaagaat atgctcaggg aaccagcaaa aataagtcgt cctcttctca
     5401 acatcgataa aatatagaat ttattgccaa tatacttgca aattgaaatt aattttatac
     5461 tgaaagatag attggggcaa gagccggttt ggcgattaat gattagcgag agcctacttg
     5521 acaagcacag gatcatgcga ttaaatcgcc caggtcgata agctacgaac aagcaaaaac
     5581 cagagcaggg tcgggctacc tttgtctttg gtcgtaagaa gaaacgcgaa ttgattgcga
     5641 ccgtcgtata acggctatta ccccagcctt ccaagctgga gacgtgggtt cgactcccat
     5701 cggtcgctta tatcttgcat aatatacata gaatagagca tcttgttcat gcagaatgct
     5761 gccgtatcta gctgatccga gtctgttgcg ataaatatcc caaataaatt aaaatataaa
     5821 aagatctttt gcttttcgat cgaaataaaa gatactattt gccgatgagg tgcttggctc
     5881 agtttccctg gtgagtataa tgaattcggg aataacgatt atgaaaccaa aagtaaagac
     5941 acctattgtg gagcttgatg gcgatgagat gacaagggtc atctggtcga tgatcaaaga
     6001 aaaactcatt ctcccctata ttgatgtcca tcttgaatat tacgaccttg gtgttgcgaa
     6061 tcgagatgtg acaaatgatc gagtcacgat ggacgcggct cgagccattg ccaaatatgg
     6121 ggtaggagtc aaatgcgcaa ccataacacc tgactctgaa agagtcaagg aatatcgcct
     6181 caagaaagca tggccttcgc ctaatgcgac tattagggca tatcttgatg gtacagtttt
     6241 ccgcaaaccg atcatagcaa agaatatagc cccagctgtc cgcagctggc agaagcccat
     6301 cataatagga aggcatgcct atggcgatat ctacaaagca gtggattttg tggtaccagg
     6361 tccgggcacc gtaagccttg tctaccagcc ggcacaactg ggtatgccca tttctttcaa
     6421 ggttcacgaa tttgaaggcc caggagtagc tatgggcata cataataccg acgcttcgat
     6481 ccgttccttt gcccgagctt gtatagaata tgcactttca gaaaaaacag atatctggtt
     6541 tgcggctaag gataccatta gcaagaatta tcatggacac tttaaggaac tatttggcga
     6601 ggagatctcg gcgcgaagtg ccgatctaga agccgctgga attcactatc gatatatgct
     6661 cattgatgat gcggtcgctc agctgatgaa gaacgaagga ggaatcctct gggcttgtat
     6721 gaactacgat ggcgatgtct tttccgatat ggtagcctct ggatttggca gccttggcat
     6781 gatgagttca gtccttgtat ctccttcagg agctttcgaa tatgaggctg cgcacggtac
     6841 tgttcgtagg cactattatg aatacctcaa gggaaatcct acgagcacta atcccattgc
     6901 gtctatcttt gcgtggacag gagcgcttag aaaacgtgct gagctcgata aaacacccga
     6961 gataacaatc tttgcgcaac agcttgaaga tacggcaatt gaactgatcg aatcgggtgt
     7021 ggtcacaaag gacctcataa accttataag cccacctgca aagagctttg aaacaactga
     7081 atcatttttg gataagatcg atgctggcct cgcccggaag cgaaccgcat tgcaggctta
     7141 attgaaaagc tagatcccta gatgcttcaa gtcttgtggg tctaaatacc ttctacaagg
     7201 aaatgacaac aaagttcaag taacagaact caggtaacaa tgggtatttg ctagaacaat
     7261 attatgtaat tccttatggg atcagcgcga ctcgaacgcg cgacctactg cttagaaggc
     7321 agttgctcta tccagtt
//
LOCUS       DAOMZL010000079         7148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_9815_length_7148_cov_16.071902, whole genome shotgun sequence.
ACCESSION   DAOMZL010000079 DAOMZL010000000
VERSION     DAOMZL010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7148
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9815_length_7148_cov_16.071902"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..1726)
                     /gene="mtaB"
                     /locus_tag="PLQ30_06625"
     CDS             complement(251..1726)
                     /gene="mtaB"
                     /locus_tag="PLQ30_06625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPW02156.1"
                     /translation="MAMLRVAFQTLGCKLNQLETESIADKFLDAGAEIVPFHEEADLY
                     IVNTCTVTSKAEQKARHDIRSAIHKVRNTTVIATGCYAQMDSEDLSLLDERVIVISGD
                     LKASLLLLPQWLLANWQGHGDLFHALLEWRDEILAAAKGSEALSNESGKTNTIDLSLR
                     NRFAFHPERFLMHSRPALKIQDGCNNRCTYCRVCLARGPSVSLPVEDALSRVIDLERA
                     GKTEVVLTGVNLAQYRAGGLDFAGILASMIKETSNIRFRISSYEPENIDEAFLATFAH
                     PRVRPHLHLSVQSGCTETLRRMGRPYSAQDVRFAVQNLRSVRSDPFLAADIIAGFPAE
                     TESEFENTIALLSELDFAWIHAFPFSPRQGTRAADMKPRIPERIACERVDRLLELAKH
                     GKRSYIERWLGHEVEMVIEHEASADKHNGDENRATDYASARIVGTTENYLKAMIEASE
                     RVEQSIMPGRAGDSVKVVLHSKGESALADGAIDVLATISSK"
     gene            complement(1745..2425)
                     /locus_tag="PLQ30_06630"
     CDS             complement(1745..2425)
                     /locus_tag="PLQ30_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW02157.1"
                     /translation="MIYQANGIGRIALIGMFFLSIWISGLFGQSPSYSISVPTGMTGS
                     DLLRMSQEGKHNDVALLAPQAIAKNPQDVDAYIGLAWSLNALKEYAKARDIAKQGYSK
                     FKDPRLAQALGEAQYYLGENEAALANLQQYLAKFPEGSKTALTFYLCGELFVRMSKLN
                     HADIAFSTALQYNPSNARWWARLGWVRERTARYIPALKAYEQAISLNPNLEDALNGKS
                     RMLAKIED"
     gene            complement(2422..2775)
                     /locus_tag="PLQ30_06635"
     CDS             complement(2422..2775)
                     /locus_tag="PLQ30_06635"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPW02158.1"
                     /translation="MAEEVKLSVVDEEEVDTVLGSEIEFEGDIESSKSLMIKGKVSGN
                     INCSAELYITEQAEVRSTIHAATVVIRGKVYGDISADSLIAVLDWGHVEGCLVAPDIY
                     LSPNCVFKGTTVIKS"
     gene            complement(2807..3703)
                     /gene="rsmA"
                     /locus_tag="PLQ30_06640"
     CDS             complement(2807..3703)
                     /gene="rsmA"
                     /locus_tag="PLQ30_06640"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPW02159.1"
                     /translation="MSQNEINYDSPASIQAFLVQNGLAVSKRLGQNFLIDRSVRSRIV
                     KALEVEEGMHVWEIGPGIGAMTTLLLDEGVRLSAFEIDYGFVRVLEGLFSANENFSII
                     KGDVLKTLCSQKEPPERIFGNLPYNSAFAIISDILEMEFLPQRMIFTLQKEAAQRMVA
                     GPGTKDYSSLSVFCASVCDVRILFDIGSSAFWPRPRVTSSVVQLIPKKKSMSASERKD
                     FSDFVRAVFSSRRKTLRNTLRFWMKQRSPSLREEEIDTRLRAILEELLIDPSIRAEVL
                     TPDELFAVYRQLHAILSSCASC"
     gene            complement(3740..5563)
                     /locus_tag="PLQ30_06645"
     CDS             complement(3740..5563)
                     /locus_tag="PLQ30_06645"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPW02160.1"
                     /translation="MRGFYCSRWFRCAKVRTRIIRRYCLQKVPAGCFAAVGLWIGLGL
                     NGLGLDSLEIGPYFEIGLGSLQLVFAIILNAAALTWLEFIGTIGKCSRGKQSFIIISA
                     PSNLSTRSNKRDVLLWSALSLGLALSAGVLRFQDQEASFLAPAEPATEASAVASHAAT
                     AASIVATPPAQTPIIAAPEASANLRIEALEGMLVLDSQTTARKNRMLTLKVQAAEYVG
                     PNMRIRSDWKRPLFSQVLFSGAGARFCSGSLIRAESVRTGDFCWVDASKLKWKAWASG
                     PARFRSIITGRFAESIATAAGKAGALAQALLLGVKDELDNEFKELFQAAGCAHLLALS
                     GQHLSIICGLVSLLGRRIIRKEKMVRRLSLAFAWFFVWLAGPGPSLLRSVFMLSCAEI
                     AREIDRPQSGFAILSLAAILLALFSPSSINSLSSIYSFSAMAGLMAFGSRFLIILRHW
                     LPDWLARAFSASFAAVCGTAPVSLLTFGTFIPAGIFAATAAAPVMLVFMWIALVGGLI
                     AVPLAFVSRITAPLLEALQSVLTWILASSASFPIFNVGSIPMLRILSCLLVVCFITLI
                     YAIPWIVWCKSQRKIEELQGRMPFLRSKNMYAILNPPDEAQ"
     gene            5658..6326
                     /locus_tag="PLQ30_06650"
     CDS             5658..6326
                     /locus_tag="PLQ30_06650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPW02161.1"
                     /translation="MINLSPKRISAYGTILFVYATVWADIILAIPEPTAFSAAWFILS
                     VLKNCIRIGFMLYISSQLPIFANNEWKRALLRLPTRREIARALSVTFLSLGVAAIGAI
                     AAWFCALSNPVFEFIPQKSILSLLPFLLLSSVSVGYSEELFFRFLLIDALTEADTALN
                     SAAIVSILIFSLSHYAQGIFGIAFAGVLGALYTRLRFRGYGIHSLALGHALYDAAILA
                     LALS"
     gene            6390..6749
                     /locus_tag="PLQ30_06655"
     CDS             6390..6749
                     /locus_tag="PLQ30_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02162.1"
                     /translation="MKPEIKTINDLLDALNLISTRAWTPKELSRAIEQALSTLPRSVI
                     EIMIGLAGEKIRSSPLRGPLVIATFSSVLLKQYDGTPLTLSEWRELRDLVSDCADELD
                     MEIVTYAMSLIMDYGAL"
     gene            6764..>7148
                     /locus_tag="PLQ30_06660"
     CDS             6764..>7148
                     /locus_tag="PLQ30_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02163.1"
                     /translation="MSFCSNPILKALILSLILATAAILPLSAVDIDSPLSESSEGAFS
                     GSDPNAIQSPQISEPDAEKAPESKPPEILEPEPSKKLEPEPALPPSLFLPILTEPFDP
                     SGFFFAAEKIRTQASEQKTNLTEDQQ"
BASE COUNT         1897 a         1837 c         1598 g         1816 t
ORIGIN      
        1 ccaagattca tggtcttata cacattggtt atcattgcat cacagcccga aaagagcaac
       61 gcgactatta tgagtaacgc aaaacatgca acatgtttct ttcgcataaa tacctccttt
      121 ttgtcattca tgcgatctcg acttaactcc acaacttgac aactctacgt aatatattct
      181 agctcaattt ggcaataaat caagaagcaa aaaacgtaga gcccgaataa aactggtctt
      241 ggaagccctg ttattttgac gagattgtcg ccagcacatc tattgccccg tccgccaatg
      301 cgctttcgcc cttgctatgc aacactacct tgaccgaatc tcctgcgcgg cctggcatta
      361 tactctgctc taccctctcc gacgcctcga tcatagcttt aagataattc tctgtggtac
      421 caacaattcg cgcagatgca taatcagtag ccctattttc atcgccatta tgcttatcgg
      481 cactggcttc atgttcgata accatctcga cctcatgccc aagccatcgc tctatataag
      541 agcgcttccc atgcttggcc agctcaagaa gcctgtcaac gcgctcgcag gcaattcttt
      601 ctgggatacg cggtttcatg tctgcagcgc gcgtaccttg tcttgggcta aaaggaaaag
      661 cgtgtatcca ggcaaaatcg agctcagata gcagggctat agtgttctca aactcagact
      721 ccgtctctgc agggaagccg gctattatgt ccgctgccag gaaaggatcg cttcggactg
      781 aacgcaggtt ctgtacagcg aacctaacat cttgggcgga gtatggtctt cccatgcgtc
      841 ttagtgtctc agtacagccc gattgcaccg agagatgcaa gtgggggcga actcgcgggt
      901 gggcaaaagt cgccagaaag gcttcatcga tgttctcagg ctcataactt gagatccgaa
      961 acctgatatt gctcgtctct tttatcatag aagcaagaat gcccgcaaaa tccagccctc
     1021 ccgctcgata ctgcgcgagg tttacgcctg taagaacaac ctccgtcttg ccagccctct
     1081 ctagatcgat cacgcgggag agtgcatcct caaccggcaa ggataccgaa ggacccctag
     1141 caaggcacac gcggcaatat gtgcaacggt tattgcatcc atcttgaatc ttgagtgcag
     1201 gacgagaatg cataaggaat cgctctgggt ggaacgcaaa gcgattgcgc aaggacaggt
     1261 ctattgtatt ggttttgccc gattcattcg acagcgcctc cgatcctttg gccgcagcta
     1321 gaatttcatc gcgccattct agtaaagcat ggaaaagatc gccgtggcct tgccaatttg
     1381 ccaaaagcca ttggggaagc aaaagaagcg aggctttcag gtctcccgaa ataacaatga
     1441 ctctttcgtc cagcaaagaa agatcctcgg aatccatttg agcataacaa cctgtggcta
     1501 tcactgtagt gttacgaacc ttgtgtatgg cggagcggat gtcatgccgc gccttttgtt
     1561 cagctttgga tgtcaccgta caggtattta cgatatagag atcggcctct tcatgaaatg
     1621 ggacaatctc ggcgcccgca tccaggaatt tgtccgcgat ggattcggtt tcaagctgat
     1681 tgagcttgca gccaagagtc tgaaatgcta cccgtaacat tgccattcgg gaatttctcc
     1741 gccctcaatc ctcgatcttc gccagcatgc gcgattttcc gttgagggca tcctccaggt
     1801 taggatttag gcttatggct tgctcgtagg ccttgagcgc aggaatatag cgagccgttc
     1861 tttccctgac ccatccaagg cgtgcccacc atcgagcgtt gcttggattg tactgcagag
     1921 ccgtgctgaa tgctatgtcg gcatgattta atttgctcat gcgcacaaaa agttcaccac
     1981 ataaatagaa tgtaagggca gtcttgcttc cttctgggaa ttttgccaga tattgctgta
     2041 aattggccag tgcggcttcg ttttctccaa gataatactg agcttcgcct aaggcctggg
     2101 caaggcgcgg atccttgaac ttagaatagc cttgcttggc aatatccctg gcttttgcat
     2161 attcctttaa ggcatttaga ctccaagcaa ggcctatgta tgcatctaca tcctgaggat
     2221 tcttcgcgat tgcttgtgga gcaagaagag caacgtcgtt atgctttcct tcttggctca
     2281 ttcgaagaag gtccgagccc gtcatgccag ttggtacact tatcgaataa gatggcgact
     2341 gaccaaatag tccggaaatc caaatagata aaaagaacat cccaataagg gctatccgcc
     2401 caattccatt agcctggtat atcatgattt gataaccgtt gtccctttga aaacgcaatt
     2461 tggcgaaaga tagatgtcag gggcaaccag gcacccttct acatgtcccc aatcgagcac
     2521 ggctataagg gaatctgcgc ttatatcacc atataccttc ccgcggatca cgactgtggc
     2581 agcatggatg gtcgatcgca cctcagcctg ctcggtgatg tagagctcgg cgctgcaatt
     2641 gatatttccg ctcacttttc ccttgatcat gagggatttc gaggattcaa tatcaccctc
     2701 gaattctatc tccgagccga gcactgtatc gacttcttcc tcgtcgacca cgctgagttt
     2761 tacttcttca gccatcttgt caatataata aagatctagg ccaatctcag caagaagcac
     2821 aggatgacag aatagcatgc aactgtcgat aaactgcaaa aagctcatcc ggtgtaagga
     2881 cctctgcgcg gatggaagga tcaattagca gctcttccaa gattgcacgc aatcgagtat
     2941 caatttcctc ttcacgcaga gaaggagagc gctgcttcat ccaaaagcgc aaggtatttc
     3001 taagtgtttt cctccgagat gaaaaaaccg cacgcacaaa atcggaaaaa tccttgcgtt
     3061 ctgaggcgga catcgatttt ttctttggaa taagctgaac aacagagcta gtcactcttg
     3121 gccttggcca aaatgcgctc gaaccaatat cgaaaagaat tcgaacatcg cataccgagg
     3181 cacagaacac gctgagcgag gaataatcct tagtccccgg tccggctacc attcgctgtg
     3241 cagcttcttt ttgaagggta aaaatcatgc gctgaggcag aaattccatc tcaagaatat
     3301 ccgaaattat agcaaacgca gaattatatg gtagatttcc aaatatgcgc tcaggtggct
     3361 ccttctgcga gcatagcgtt tttagaacat ccccctttat aatagaaaaa ttctcatttg
     3421 cagaaaaaag accttcgagt accctcacaa atccataatc gatctcaaaa gcagacagtc
     3481 gcaccccttc atcgagaaga agtgtggtca ttgctccaat accggggcca atctcccata
     3541 catgcatgcc ctcttctact tccaatgctt ttacgatgcg gctgcgcacc gagcgatcta
     3601 tgagaaaatt ctgtcccaag cgcttactga cggcaagtcc attctgcact agaaaagcct
     3661 gaatcgaagc gggagaatcg taatttattt cattttggct catagaaatc gttaagcccc
     3721 cattcattaa gaccttagct tattgggctt cgtctggcgg attcagaatt gcatacatat
     3781 ttttcgagcg aagaaagggc atgcgccctt gcagctcctc aatcttccgc tgggacttgc
     3841 accagacaat ccatggaata gcataaatca gagtaataaa gcagacaacg agcagacatg
     3901 acaagatcct taacattggt attgagccta cattgaaaat aggaaaagaa gcgcttgatg
     3961 caagaatcca cgtaaggaca gattgcaagg cttcaagtaa tggcgctgtt atgcgactta
     4021 caaaggctaa agggaccgcg attagccccc caaccagcgc aatccacata aaaactagca
     4081 ttactggtgc tgcagccgtc gccgcaaaga taccagcagg aataaaggtt ccaaaggtca
     4141 agagcgacac aggagcagtt ccacaaaccg ctgcgaagga tgcggaaaag gccctggcca
     4201 gccaatccgg cagccaatgc ctcagaataa tcaaaaatcg cgaaccaaat gccataagcc
     4261 ctgccatggc gctgaaggaa tagatgctcg aaagggaatt gatcgaggag gggctaaaca
     4321 gtgcaagcaa aatagcagcc agagagagaa ttgcaaagcc tgattgcggc ctatcgattt
     4381 ctctggcaat ctccgcgcag ctcagcataa agacagatct caaaagcgag ggcccaggcc
     4441 cagcaagcca cacaaaaaac caggcaaaag ccaaggatag cctccgcacc atcttttctt
     4501 ttcggataat gcgccgacca agaagagaca cgagcccgca aatgatcgag aggtgctgac
     4561 ccgaaagcgc aagcagatgc gcacagcctg cagcttgaaa gagctcttta aattcattgt
     4621 ccagctcatc cttgactcct aggagtaagg cctgagcaag ggcgcctgct ttacctgctg
     4681 ctgtcgcgat gctctcggca aaacgacctg taatgattga ccgaaacctt gcaggaccgg
     4741 aagcccaggc cttccactta agtttggaag catcgaccca acaaaaatca ccagttctga
     4801 ccgactccgc ccgaatcagg ctgccagaac aaaaccgagc acctgcgcct gagaacaaga
     4861 cttgagagaa caatggccgc ttccaatcgc tgcggatgcg catatttggt ccgacatatt
     4921 ccgctgcctg tactttgagt gtaagcatgc gattttttcg cgcggtggtc tgcgaatcca
     4981 agacgagcat cccttcgagc gcctcgattc gcaggttggc tgacgcttca ggcgctgcta
     5041 ttattggcgt ctgtgctgga ggggtcgcaa ctatagaggc cgctgttgcc gcatgggacg
     5101 ccactgccga ggcctctgtt gccggttccg ctggggctaa aaaactcgct tcctgatctt
     5161 gaaatcgaag cacgcctgcg ctgagagcga gacctaaaga caaggcgctc catagcaata
     5221 cgtctctttt gttgcttcta gtagatagat tagatggagc tgaaatgatt ataaagctct
     5281 gttttcctct tgagcatttc cctattgtgc cgatgaattc caaccaggtc agtgcagctg
     5341 catttaagat tatagcaaaa actagctgta gactgcctaa tccaatctcg aaatacggcc
     5401 caatttcaag gctatccagt ccgaggccat tcaagccaag cccaatccac aatccgaccg
     5461 ctgcaaaaca accggccggt accttttgca agcaatatcg tcgaataatc cttgttcgga
     5521 ctttagcgca tctaaaccat cttgagcaat aaaatccgcg cattatctct ctcttttgta
     5581 atacgcacag aaattcattt ttggcttcaa aatgcgatga cagagaatta gattcgtagt
     5641 aatgtctaag caataacatg atcaacctat cccccaagcg aatttccgcc tatggaacga
     5701 ttctctttgt atatgccacc gtctgggccg atattattct tgctatacct gaaccaacag
     5761 cattttcggc tgcttggttt atcctcagcg tactaaaaaa ttgtatccga attggattta
     5821 tgctttatat ttcatctcaa ctgccgattt ttgcaaacaa tgaatggaag agagcccttt
     5881 tacgtctccc aactagacga gaaatagcca gggctctttc tgtgacattc cttagccttg
     5941 gagttgcggc cataggagcc atagctgcat ggttctgtgc cctttccaat cctgtcttcg
     6001 aattcatacc acaaaagtcg atattatctt tgcttccatt tttgctgctt tcgagcgttt
     6061 cagtgggtta cagtgaggaa ttattcttcc gttttttact catcgatgcg cttacagaag
     6121 ccgatacagc cctaaattcc gcggctatcg tctcaatact gatcttttcg ctttctcatt
     6181 atgctcaagg aattttcggg attgcattcg ccggagtact gggcgctcta tacacacgcc
     6241 tgcgatttcg aggttatggt atacactcgc ttgcgctcgg acatgcgcta tatgatgctg
     6301 caatcctggc attggctctt tcttgattgt ccgggttgag cccatgtata ggcttgtcga
     6361 taatattgat gactaacagg agatttgcca tgaaaccaga gattaaaacc atcaatgacc
     6421 tcctagacgc cctcaatctt atatcgacac gcgcatggac accgaaggaa ctctcgagag
     6481 cgatagaaca ggctttgtcc actctcccga gatcggtaat cgagataatg attggactag
     6541 caggagaaaa aattcgctcc agccccttgc gtggacctct ggttatagcg acttttagca
     6601 gcgtgcttct caagcaatat gatggaacac ccttgaccct ctctgagtgg cgagagttgc
     6661 gcgacctggt ttccgattgt gctgatgagc tagatatgga aatcgttacc tacgcgatgt
     6721 cactcataat ggactatggc gcgctgtgaa gaggattaga gatttgtcat tttgttctaa
     6781 ccccattctt aaggctctga ttctttcttt gattttggca accgcggcca tactccctct
     6841 aagtgctgtt gatatagatt cgcccctttc cgagagctcg gaaggagctt tttctggtag
     6901 tgatcccaat gctatccagt ctcctcagat ttccgagccc gacgccgaaa aggctccgga
     6961 atctaagcct ccagagattt tggagcctga gccttcaaaa aaacttgaac ctgaaccggc
     7021 cttgcctcca tctctttttc tacccatatt gaccgaaccc tttgatccat cgggtttttt
     7081 ctttgcagcc gagaaaattc gtacacaagc ttcggagcag aaaactaacc tcactgaaga
     7141 tcagcaaa
//
LOCUS       DAOMZL010000080         7102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_9908_length_7102_cov_13.925784, whole genome shotgun sequence.
ACCESSION   DAOMZL010000080 DAOMZL010000000
VERSION     DAOMZL010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9908_length_7102_cov_13.925784"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLQ30_06665"
     CDS             <1..216
                     /locus_tag="PLQ30_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02164.1"
                     /translation="LPGQHCFWPILRSYHIENLIIAPDYRNACAKLLLPCGQEFFDML
                     YCHAIIIIEIANLMQASSMHSLAKLCR"
     gene            571..1518
                     /locus_tag="PLQ30_06670"
     CDS             571..1518
                     /locus_tag="PLQ30_06670"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPW02165.1"
                     /translation="MKRILSVFIVLLLIAGAIGTVGAQAQKRYKFVVITHATAVPFFV
                     PVRKGAEDAGKLVGADVVYTGPTGFDIQQHVEFVKSAIAQKVDGIAMTMPDPNAFNAV
                     VKEAMSKGIPVIAINADAPESGRLAYIGQGNYEAGRSMGMQILKLLPNGGDVLLTIHT
                     AGAENLEDRIRGIKDILDAQGGKFKYRVVATGTDMVRAVSLISSALQANPNIKGMFGV
                     EEVTGSAIAQIIERDRLKGKVMAGGFDLVTDELVAIQKGNMQFTIDQQPYLQGFQGVM
                     ELYLLKKFNLTPCDINTGIAPVTAENVNEVMKLAAEGYR"
     gene            1568..3094
                     /locus_tag="PLQ30_06675"
     CDS             1568..3094
                     /locus_tag="PLQ30_06675"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPW02166.1"
                     /translation="MRHRLLDEKESVLQSLLSVQNISKHFGNIHALRNVSLDISPKKI
                     IGLAGENGAGKSTLVRIICGAIPCDSGEMKYQGRPYKPHDVSDAESLGISVFHQEIPI
                     CPHLSIAANVFLGPTIPSLRIRPDWKFMNNRCIELYKKFLDEDIDPTIPIGECSAAEQ
                     QLALLVRVLSRNAKLVILDEPTTALSAPEVERLFDSIKRLRDEQGIGFIFVSHLLDEL
                     MELSDEVFVLRDGENVGHLYKAEFSAKELSRLIAGRSIDVQHRSAKGFAEAEITLEAR
                     SISLSKSYEDISFSVHKGEVFGIAGLQGSGRSEVISSLFGLPPPDKGSVIIQGNDLGK
                     ITISKAITAGIGYLPEDRKTKGIFPDLNIAKNIGIVSIAQSRDLTSYNEEEYIKLVDT
                     MAGKLSIKISNPKDYISTLSGGNQQKAMLARWLALSPSILVLNEPTRGVDIGAKSEIA
                     KIISDMAEAGCTIIIASSEMEELLLMCDRIMVMNRGRARIILEKESLTRENLFFYAMS
                     "
     gene            3113..4099
                     /locus_tag="PLQ30_06680"
     CDS             3113..4099
                     /locus_tag="PLQ30_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW02167.1"
                     /translation="MNKAPTKNHRSKSNIVLGRDFGVLIAAILIAVFFTIASPVFLTA
                     YNFFNLLRQTAQLGIIAMAMTMLIISGEFDLSVGAIYAVTGVLTGILVKNMGLNIWLA
                     AFIGFLAALVLGFVNGTLISITKINSFIGTLSTQMIFRGIAMVLSQGQPISFLQSSLF
                     FDAFGRAEIFGNIPVQILWLLAWTLISFYLLHRTSFGVKVFATGDNRLAADLSGIKTT
                     RVKKTCFIITSAAAGLSSLISLAFLKTVTPTQGSGLELEAIAAGVIGGTSMSGGVGSI
                     LGTFIGTFIMAEVRTGLVLMGTDAYVQDAFVGLVIALAVIINVRFSISKGRS"
     gene            4164..4658
                     /locus_tag="PLQ30_06685"
     CDS             4164..4658
                     /locus_tag="PLQ30_06685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW02168.1"
                     /translation="MNFSIRAIAPIEAETYYSLVNEVKDEGKYLFSTIRIPLDNMKKY
                     LAQHNGSSNPVLGAFDDKGTLLGWIDINISNIEELMHIGTIAMGVRRIHRGQGIGSAL
                     LDACISQARMLGLEKLELEVFASNSVARALYKKKGFFEEGIRLKKRKFEGHYDDLVCM
                     GLFL"
     gene            complement(4940..5629)
                     /locus_tag="PLQ30_06690"
     CDS             complement(4940..5629)
                     /locus_tag="PLQ30_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02169.1"
                     /translation="MKRFLLLFAILTVFAIVPAFAQTEAELKAAYENAVKLAASAPKD
                     YTLNWQAARAARSYGDYLVVHKTTSWKETARAAAKEGMKYGEIAFNLNPSGIEGWYYY
                     GLCVGTYSDCVSVLTALTEGLKGKTQMGFENAYKFDKTYDNGGPILALGRFWQVLPGI
                     AGQDRKKAEKLFDEYIALFGSSPKANSDAWYFRGELYKDTKRPDLAKADLEKASSMGN
                     ENAKRLLGEMK"
     gene            5789..6658
                     /locus_tag="PLQ30_06695"
     CDS             5789..6658
                     /locus_tag="PLQ30_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HPW02170.1"
                     /translation="MKRMVFDKIRKMLPEMANSYQKIGSYMLDHEQSVAFSSIHSISK
                     SIGVSSATLVRFAKSLGFQGYQEFKHEIQDEIRHRLNPYDKIALSELDILPGEKRLQK
                     LFQNEYNNTRSTFANIKIADLEIMGEGIKKARRVYLSAFGATAYVAHIFKYTLMSSLE
                     KEVYVVSGSVSDYAPVLKSFSSEDVMFLMTFPPYSSEVRHVAKVVKEKGGSLFLFTDS
                     ASCPVYSEADEVIRCITNSLLLTNSFAGLISILHVLVQMVYLDEKESAAMSRQNTILM
                     QESGYLVISNPKE"
     gene            6688..>7102
                     /locus_tag="PLQ30_06700"
     CDS             6688..>7102
                     /locus_tag="PLQ30_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPW02171.1"
                     /translation="MSMLLLKNGEVYAPCPLGSRDILIGSDRIISIGSNIDLSDSIPD
                     VETIDASGCIVVPGLIDGHQHFIGGGGEGGFETRTPELQISMNFLNGVTTAIGLLGTD
                     SLTRSLEDLYAKTKAFNTEGMTAFMLTGAYWYPSPS"
BASE COUNT         2027 a         1385 c         1640 g         2050 t
ORIGIN      
        1 ctccccggtc agcattgttt ttggccgatt ttgcgttcat atcatataga gaatctcata
       61 attgcgcccg attaccgtaa tgcttgcgcc aaactcctcc tgccatgtgg gcaggaattc
      121 ttcgatatgc tgtattgtca tgcaataatc ataatcgaaa ttgccaacct aatgcaggct
      181 tcatccatgc atagtctcgc aaagttgtgc agatgattgt tgattcttgc aaagctatat
      241 caggaataga aaaagcaggc aaaactcaag atcaggatag atcgatgaat tgataaagca
      301 acaagtggtg cataatggat ttataagaaa gcaatccgag tatgcgatga ttttttgcaa
      361 aaatacttat ttgttcttga cagattatgc atcttgaagg aaaattgtca catggctgaa
      421 aacgttttta gattatcagc cactgaaggt tcttaggcat ttaacaagtc ttcctgacat
      481 ctgtgattta attagatgct tatgatttat ggttgattaa tatgatcctg ttttggggat
      541 cttaaaatca tagggcgaga ggaggacatt atgaaaagaa tactctcagt attcatagtt
      601 ctattgctta tagctggagc aataggtacc gtcggtgctc aggctcaaaa acggtataaa
      661 ttcgtggtta ttacccatgc aaccgctgtt ccattttttg ttccggttag aaaaggtgct
      721 gaagatgcag gcaagcttgt tggagcggat gttgtatata ctggcccgac cggttttgat
      781 attcagcaac atgttgaatt tgtaaaatca gcaattgcgc agaaggttga tggcattgcc
      841 atgacaatgc ccgatccgaa tgcattcaac gccgtagtca aagaggcgat gtcaaaaggt
      901 atacctgtta ttgccataaa tgcagatgcc ccagaaagtg gaaggctcgc ctatattggc
      961 caaggaaact atgaagctgg gcgatcaatg ggaatgcaga tcctaaaact gctgccaaat
     1021 ggtggagatg tgcttttaac aattcatact gcgggtgcag agaatcttga agacaggatt
     1081 cgcggcatca aagatatact cgatgctcaa ggcggtaagt tcaaatatcg agtggtcgcg
     1141 acaggtactg atatggtacg tgcagtctct ctcatttcta gcgccctgca agcaaaccca
     1201 aacatcaaag gcatgtttgg agtggaagaa gtaacaggga gcgcaattgc ccaaatcatc
     1261 gagagagatc gcctcaaggg gaaggttatg gctggtggtt ttgatcttgt tacagatgaa
     1321 cttgttgcaa tccaaaaagg taatatgcag tttactattg atcagcaacc gtatctccag
     1381 gggttccagg gtgttatgga gctatatctt ctcaagaagt tcaatcttac tccatgcgat
     1441 ataaatactg gtattgcacc tgtcacggct gaaaatgtaa atgaagtcat gaaacttgca
     1501 gccgaaggct atcgttagaa ttcatttggc gtcataaaaa ttggctcttc catctggaga
     1561 cggtgtcttg aggcaccgtc tcctcgatga aaaggagagt gttttgcaga gtctattatc
     1621 tgtacaaaat atctcaaagc attttggcaa tattcatgct ctgagaaatg tatctttaga
     1681 tatttcacca aaaaaaatta tcggtctggc cggcgagaat ggcgcgggca aatcgacgct
     1741 tgtccgaatt atttgcgggg ctattccttg tgattcagga gaaatgaaat atcaaggtag
     1801 accatataag cctcatgatg tatcggatgc cgaatctctg gggataagcg tttttcatca
     1861 agagattcct atttgtccac atctttcaat tgcggcaaat gtgtttcttg ggccaactat
     1921 cccttctctt agaataaggc ctgattggaa attcatgaat aatcgctgca tagaattgta
     1981 taaaaaattc ctggacgaag atatcgatcc gaccattcca attggagaat gttctgctgc
     2041 cgagcagcaa ttggctcttc tggtcagagt attgtctcga aatgcaaagc ttgtgattct
     2101 cgatgagcca actacagcat tgtctgcgcc agaagtagaa agactttttg attcgataaa
     2161 aagactgaga gatgagcaag ggattggttt catatttgta agtcatttgc tcgatgaact
     2221 tatggagcta tccgatgagg tttttgttct aagagatgga gaaaatgtag gccatctcta
     2281 caaagccgag ttttcagcca aagaattatc gaggcttatc gctggaagat caatcgatgt
     2341 gcaacatcgc agcgcaaagg gttttgccga agcagagatt actcttgaag caagatccat
     2401 ctcattatcg aaatcttacg aagatatttc tttttccgtc cataaaggtg aagtctttgg
     2461 tatagcgggc ttacagggat cgggaaggtc cgaagtcatt tcgagtcttt ttggattgcc
     2521 gccgccagat aaaggctctg tcattatcca aggtaatgat cttgggaaga taactatatc
     2581 taaagcgatt accgcgggta ttggttactt accagaagat aggaaaacca agggcatttt
     2641 cccagaccta aatatagcaa agaatattgg cattgtgagt attgctcaat caagagatct
     2701 aactagctat aatgaggaag agtatataaa gctagtagat actatggccg gcaagctttc
     2761 tataaagatt tctaatccta aagattatat atctaccttg agcggaggaa atcagcaaaa
     2821 ggctatgctt gcacgttggc ttgctctatc tccatcgatt ctcgtactca atgaacctac
     2881 cagaggcgta gatattgggg ctaagagcga gattgccaag atcatatccg atatggccga
     2941 ggcaggctgt acgataataa tagcatcgtc agaaatggaa gagcttttgc ttatgtgcga
     3001 tcgaattatg gttatgaatc ggggtagggc aagaattatc cttgaaaagg aatctttgac
     3061 aagggaaaat cttttctttt atgcaatgag ttaaataaaa aaggaagggc tcatgaacaa
     3121 agcaccaacc aagaatcata gaagtaaatc caatatagta ttggggcgcg attttggcgt
     3181 tctgattgct gctatattga ttgcagtttt ttttacaatt gcttcgcccg tctttcttac
     3241 tgcatataat ttttttaatc ttctgcgaca gaccgcccag ctcggcataa ttgccatggc
     3301 catgacaatg ctcattattt ctggggaatt tgatctctct gtaggagcaa tatatgcagt
     3361 gacaggggtc cttaccggaa tactagtaaa gaatatgggt ttgaatattt ggttagctgc
     3421 atttatcgga tttctagctg ctcttgtcct tggcttcgtt aatggtactc tcatttccat
     3481 tacaaaaatc aactcattta tcgggactct ctcaactcag atgatattcc gaggaattgc
     3541 tatggtgcta tctcaaggcc aaccaatatc gtttctacag agctctctgt tttttgatgc
     3601 ctttgggagg gctgagatct ttggaaatat tccggtacag attttatggc ttttggcatg
     3661 gacattgatc tcattttatc tcttacacag gacatctttt ggagtgaaag tatttgccac
     3721 aggcgataac cggcttgcag ccgatctttc aggaataaag acaactcgcg tcaagaagac
     3781 atgctttatt attacatccg ctgcagcagg tttatcttcg cttatatccc tggcttttct
     3841 aaaaacagtt acgccgactc aaggctctgg tcttgaactg gaagcgatag ccgcgggagt
     3901 tattggagga acttcgatgt cgggaggggt tggaagtatc ctggggacct tcattggtac
     3961 atttataatg gctgaggtca gaacagggct ggttttgatg ggtaccgacg cctatgtaca
     4021 ggatgccttt gtaggattag ttatcgcctt ggccgttatt atcaatgtga gatttagcat
     4081 ttccaaaggt cgctcctgag ccgcaaaatg ctaaacactt gaaaaagaac ccgattgagg
     4141 attaatatcg gcagttgata atcatgaatt ttagcattcg cgctatagct ccgatagaag
     4201 cggagactta ttattccctt gtcaacgaag taaaagatga agggaaatac ctattttcga
     4261 ccattcgcat tccgctcgat aatatgaaga agtaccttgc ccaacacaat gggtcttcca
     4321 atccggtgct aggggctttt gatgataagg gaacacttct aggctggatt gacataaaca
     4381 taagcaatat cgaggagctt atgcacattg gtaccattgc tatgggtgtt cgaaggatcc
     4441 atagagggca aggcattgga agtgctcttc tcgatgcatg catttctcaa gcaagaatgc
     4501 ttggtctcga aaaattagaa cttgaggttt ttgcctcaaa ttcagttgcc cgcgctcttt
     4561 ataagaaaaa aggctttttc gaagaaggta ttcgtctcaa gaagcgaaag ttcgaaggac
     4621 attacgatga tttggtctgt atgggtctgt ttctatagag ataaatcctt ctgcataaat
     4681 acctttgtga aaaatgagtt ctgcgagcct tctgctatac ttgatgcaaa atgccattcg
     4741 accttttcca taacttggag aatgcccttt tgtctggcat aatgagcggc ttctctatga
     4801 tcttcggcga aatacaaatc gatttaatgg ttagggctcc tggcatctaa taaagtacag
     4861 atagttcttt ccagccagtc ttatcgttgt ggaattcgtt ctgtgtcaga aatattgccc
     4921 taaaattggg ccccaaacct tatttcatct ctcctagcag tctctttgcg ttttcattgc
     4981 ccatcgagga tgctttctcc agatcggcct tggccaagtc tgggcgctta gtgtctttgt
     5041 agagctcacc gcggaaatac caggcatcgc tattggcctt aggagaggat ccaaataagg
     5101 caatatactc atcgaatagt ttttcggctt tcttgcgatc ctggcctgca attcccggca
     5161 agacctgcca aaagcgacct aatgccagaa ttggaccacc attatcgtag gtcttatcga
     5221 atttataggc attctcaaag cccatttgcg tcttgccttt tagcccttcg gtcaaggctg
     5281 taagtacact gacgcaatcc gagtaggttc ccacgcaaag gccatagtag taccatcctt
     5341 cgatgccgct cgggttcaga ttaaaggcga tctccccata tttcatgcct tctttagctg
     5401 ccgcgcgagc ggtttctttc cagctggtag ttttgtgtac aacgagataa tcaccataag
     5461 atcgcgcggc gcgggcagct tgccaattca aggtgtaatc cttgggggcg gaagcggcta
     5521 gtttgacggc gttttcgtaa gcagccttaa gttcggcttc ggtttgtgca aaagcgggga
     5581 caatggcgaa gacagttagg attgcgaata atagcaaaaa gcgtttcatc aaagatgcct
     5641 ccttgtgaaa aagctatcct gctgtgagtt agcggtcaag agtccttaac tgcaaaaaat
     5701 tgaaaaaact tgccgataaa cgttgacata taaatttaat ggtgcgataa tcccgaaaca
     5761 aaagttacca agattagatc tggagaaaat gaaacgcatg gtttttgata agatcaggaa
     5821 aatgcttcct gaaatggcca atagctatca gaagatcggc tcttatatgc tggatcacga
     5881 acagagtgtt gctttttcgt caatccactc gataagcaag tccattggcg taagcagcgc
     5941 gactcttgtc cgattcgcaa aaagcttggg gtttcaagga tatcaggaat tcaagcatga
     6001 aattcaagat gaaataaggc acagattgaa tccttatgac aagattgctc tgagcgaact
     6061 ggatatcttg ccaggggaaa aacgtctgca aaagttattc cagaatgaat acaataacac
     6121 ccgcagcact tttgccaata taaagatcgc tgatctggag ataatgggag aaggcataaa
     6181 gaaggctcgc agggtatacc tgagtgcatt tggggcaacc gcctatgtgg cacatatttt
     6241 caaatatact ttgatgagtt ctttagaaaa agaagtatat gtagtttcgg gctcggtctc
     6301 ggattacgct ccggtgctca aatctttttc gagcgaagat gttatgtttc taatgacatt
     6361 tcctccatac tcgtcggaag taaggcatgt ggcaaaagta gtaaaagaaa agggcggttc
     6421 gctgtttctt tttactgatt cagcttcatg tcctgtatat tcggaagcag atgaggtcat
     6481 acgttgtatc actaattcgc tattgttgac caattctttt gccgggctta tatcgatttt
     6541 acatgtactg gtgcaaatgg tgtacctaga tgaaaaggaa agcgcggcta tgagtaggca
     6601 gaacaccata ttgatgcaag aaagcgggta cctggttatc agtaatccga aagaataata
     6661 aatccaatgc aaattatgga gcacaagatg agcatgttat tgctgaaaaa tggagaagta
     6721 tatgcaccct gtccgcttgg atccagggat attttgattg gaagcgacag gattatctca
     6781 attggttcta atatcgattt atccgattcg attccggatg tcgaaacgat agatgcatcg
     6841 ggatgtatcg tcgttccagg attgatagat gggcaccagc attttatagg cggcggaggc
     6901 gagggaggat ttgaaaccag aactccagaa cttcagatat caatgaattt tctaaacggt
     6961 gttactacag ctatagggct cttgggaaca gactctctaa ctcggtcgct cgaagatctc
     7021 tatgcaaaaa ccaaggcttt caataccgaa ggcatgacgg ctttcatgct aacaggagct
     7081 tattggtacc cttctccaag ca
//
LOCUS       DAOMZL010000081         6977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_10153_length_6977_cov_13.438602, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000081 DAOMZL010000000
VERSION     DAOMZL010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10153_length_6977_cov_13.438602"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..114)
                     /locus_tag="PLQ30_06705"
     tRNA            complement(35..114)
                     /locus_tag="PLQ30_06705"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(78..80),aa:Leu,seq:cag)
     gene            complement(319..2067)
                     /locus_tag="PLQ30_06710"
     CDS             complement(319..2067)
                     /locus_tag="PLQ30_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPW02172.1"
                     /translation="MKERIVSGLFPIFSIIPSLSDLPKNQSIRDPVIRVSDLTITAPS
                     IFTEETFGELDFYLFVPNKAKDIEPKAAGLAIILMDNDSLAQVAYEFAKASTQAIELT
                     IVAAIKKPKSGYDIEIINELVSFLRSKYSIAETKIWSVGEGAGAPACIALNRAYPSAF
                     ASVAVNIDVEAFEGPWLATKPVLPGSDYKVVDVPKWTVTKQMERTINWTKKHPDYITV
                     TASSNQQDAILATDKDYCGPTAWKATEPGPSWISIDFGEPLYITRWAVLATEVNDNYA
                     LEYSDNGVNWSGLDAISSNKNARIDRFVRRGVLAQYYRLSSTNPMSIREFEVYGDHCS
                     FDAFGYRLYNGRNGSYLHFRIFKPNRAYYRNDTKVPLVVFLHGAGQRGHYNNAQLGIT
                     TGEGATNIAYDWWQKKHPCYIIAIQCPLEEAHFFEQIAANEVDIIKQVIAEFPDIDKD
                     RVYVTGLSLGGFGTYKLIVDYSDIFAAAVPLAGGGFDNGKGPTDPDNYISRVSNIKNM
                     PIWAFHSEDDDLVSIKLGISMVEAIKAIGNTNIRFTVYPKGTFGFTPHYCWLFAYYDW
                     DMWEWLFAQNRKFWAK"
     gene            complement(2287..3447)
                     /locus_tag="PLQ30_06715"
     CDS             complement(2287..3447)
                     /locus_tag="PLQ30_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW02173.1"
                     /translation="MKRQIVALLLILVAVSFGFSQTKVVVNEWRIPFLNCLTGPIAAI
                     GEFLQWGANYAANEINAAGGIAGKPVRVIPVDTGNDPQKGSVEMARIVKDSLVAMGPV
                     PEPVILAAMPIAVENQMMSITATTSYEYAVQFFPWTVSWFPPTLDRLPPLVAGWAKQF
                     SDLKQVVQFIEPYGPWPGMADAHSKALTSLNIKELKAIEVPTDAVTFGPLVVRALDQK
                     PDGIILVCNAEKAAKIIKELKSRGWTKMNHILVFSSADDAQLYSTGGADLNGVQVYNY
                     LNLEVSNPRWDKFKAAFAKDHNGQMPFSLSPNYYDALYMIKEAIEKTGVTGDPKKLKE
                     ERKKIADYCANVKGFQGLLYEWDMKDGVPTNKPSYIFEIQNGVKQFVLEVRP"
     gene            complement(3444..4349)
                     /locus_tag="PLQ30_06720"
     CDS             complement(3444..4349)
                     /locus_tag="PLQ30_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPW02174.1"
                     /translation="MPLSLYYFLSITAVNILMSWALYVPYRVTQLHFITIANMMISGY
                     LAGYLVLHAHVPFIWALLIGFAVGGIIGFIVSLFIGDAPTFSVVIVGFTSIYITRTIV
                     ENLPIIGGTMGMFGLPNIGGNPGTHRWIILCILYGLVILCGFLIYRFDHSSIGRAASA
                     VFVDKELASSLGIDIKKLGMLVQTFSCTLAGGVGVLYGFIYRSFHLDFFGFALIGIFM
                     TVLFVGGYSTQWGPLITAPILYGLPLFFPTEVQSWRIVIYGLLLILVLELKPEGFITR
                     KFVHSLGLSFSRKRAKEIYSKEEEQ"
     gene            complement(4359..5243)
                     /locus_tag="PLQ30_06725"
     CDS             complement(4359..5243)
                     /locus_tag="PLQ30_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPW02175.1"
                     /translation="MQVFIAQILNGLSIGSVYMLLATAFNLLLLVAMIIHFSYPQVVV
                     FCMYIIWLVLKITNNNVFFGILAGVSSAILFNIISAPLFQKIMKNREEVDINATMVLS
                     MAIGWIITDVLSHAFNNGLPIQFPEEITGSKPLFRVGLISILPGQVLALSIGIIVVVG
                     FFILLYKTKMGRAFRAIAEDPAGAKLVGIPLVKTGFQSYALSGALGGLTAVLLIMLFH
                     SASPGLGEQVSHKVLAVSIVAGLGNLVGGLVVGLALGILEALVQGYVSGNWSNAIAFV
                     VMLVIILVKPRGLFGVKV"
     gene            complement(5246..5944)
                     /locus_tag="PLQ30_06730"
     CDS             complement(5246..5944)
                     /locus_tag="PLQ30_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW02176.1"
                     /translation="MLTVEEISVSYGHIQALKKISIEVYPQEIVVLIGANGAGKTTLL
                     SSILGVKTPSSGKVVFEGKRIDKASVDRNVRAGLCLVPEGRGVFASMSVLDNLLLGAH
                     HNLANKDKNLARVYEWFPFLYKRKDQIARTLSGGERQMLAIARALMSDPKLIMIDEPS
                     LGLAPIIVNSIFDILVTLNREGYTVLLAEQNAFKALNCAHRGYVLETGTISLSGPSSR
                     LLNDPGVKAAYLGA"
     gene            complement(5973..6728)
                     /locus_tag="PLQ30_06735"
     CDS             complement(5973..6728)
                     /locus_tag="PLQ30_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW02177.1"
                     /translation="MLKISGLSKSFGALQALDKVDIEIKEGEIHGIIGPNGSGKTTLF
                     NCITGILKPNEGKVIFKGEDITGLKPNIIANKGIHRTFQAGKLVSNLTVLENIMCGFQ
                     EPLSKSLKDTMIRLPFTQSKREKEIKEKAERIAEFVGIVDSIHRWASDLVWTERQLVQ
                     IGRALINEPQLLLLDEPASGMGSLEIEKMEEIIRKIRKKGITIVVVSHEVRMLMNLSE
                     IVTVLSFGQKIAEDIPENIQKDPRVLEAYLGTK"
     gene            complement(6754..>6977)
                     /locus_tag="PLQ30_06740"
     CDS             complement(6754..>6977)
                     /locus_tag="PLQ30_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02178.1"
                     /translation="TGKDPIWNGVPGATDGTFLSAWKNIPCIVNGPGPRHIPHQIDEY
                     VEIDEAYECAKIYALTASRFLASKSSDNP"
BASE COUNT         2016 a         1622 c         1325 g         2014 t
ORIGIN      
        1 tttgctataa ttatttgctt attatatatg aatttgccca gagggggact tgaaccccca
       61 tgctttcgca ctagaacctg aatctagcgt gtctgccaat tccaccatct gggcgggttt
      121 gctgctgcaa tttcgcattg taattcagtc gatactcgct ttccgttctt aggactcagc
      181 gattgtacgg ttttgtaatt gtgcagctcg actagcgcgg ctgtatttat aacgcaggca
      241 atggattgat gtcaagggaa gacaaagaca tgctgcctgg ctaccttctc gattgccttc
      301 tattccttac taaagctatt atttggccca gaactttcta ttttgggcaa aaagccattc
      361 ccacatatcc caatcataat aagcaaaaag ccaacaataa tgcggggtga agccaaatgt
      421 ccctttagga taaacggtaa acctaatatt tgtgttacca attgctttga tagcttccac
      481 catagaaatt cccaacttta tactcaccaa atcatcatct tcagaatgaa aagcccaaat
      541 aggcatattt ttgatattag atactctaga aatatagtta tcaggatcag ttggaccttt
      601 gccattgtca aaaccgccac cagctaatgg aacggcagca gcaaaaatat cactataatc
      661 cacaattagt ttataagtcc caaagccacc taatgataga ccagtaacat agacacggtc
      721 tttatcaatg tctggaaatt cagcaattac ttgtttgata atatcgacct catttgcggc
      781 tatctgttca aaaaaatgcg cttcctctaa tggacattga attgcaataa tgtaacaggg
      841 atgtttcttt tgccaccaat cataggcaat attagtggca ccttctccgg ttgttatccc
      901 tagctgtgca ttgttataat gtcccctttg gcctgcgcca tgtagaaaaa ctacaagggg
      961 gaccttagta tcattacgat agtacgctct attgggcttg aatatccgaa aatgtaggta
     1021 agaaccattt ctgccgttgt ataatctata gccaaaagca tcaaagctgc aatgatcacc
     1081 atacacttcg aattctctaa ttgacatggg atttgtagag gagagtcggt aatattgcgc
     1141 aagaacgcct cttcttacaa atctgtctat tcttgcgttt ttattgctgc taatagcatc
     1201 caatccagac caattgacgc cgttgtcact atattcgaga gcataattgt cgttaacttc
     1261 tgttgctagc actgcccatc tggttatgta aagaggttct ccaaaatcaa tgcttatcca
     1321 actaggacct ggttccgtag ctttccatgc tgttgggccg caataatcct tatccgtggc
     1381 cagtatggca tcttgctgat tgctcgaagc ggttactgtt atataatctg gatgtttttt
     1441 tgtccaattt atggtgcgtt ccatttgttt agttacagtc catttgggaa catccaccac
     1501 tttataatca gaccctggca aaacaggctt tgtagcaagc caaggccctt caaaagcttc
     1561 cacatcaata ttgactgcaa ctgatgcaaa agcagaagga taagctctgt tgagagcaat
     1621 gcatgctggt gcgcctgctc cctctcctac gctccatatt tttgtttcag ctatgctata
     1681 cttgcttctt agaaaactaa ctagctcatt gatgatctct atatcatatc cagatttggg
     1741 tttttttatt gcggcaacaa tagtgagctc aatagcttga gtagaagctt ttgcaaattc
     1801 atatgctacc tgtgctaagg agtcattatc cattagtata atcgctaagc cggcggcctt
     1861 tggttcaata tcttttgcct tgttgggaac gaaaaggtaa aaatccaatt ccccaaaagt
     1921 ctcttctgta aatatagaag gagccgtgat agttaggtca cttactctga ttacgggatc
     1981 tctaatagat tgatttttag gcaaatcaga taaggaaggt atgatactga atatcggaaa
     2041 taatccagac actattcttt ctttcatttc cagcccccta taatgtccaa ttcgccatcc
     2101 aagagctttg tatctttgtt tccataatgc taacacatgt aaatgctccc ctcaataatc
     2161 catttgatcc gttcaagaga ttgcaaaaca ctggcaaaat cgattttaag cactgtatat
     2221 agaaagggcg ccgcatgcgc ggcgcccctt cttgatttat tcacactccc ttataaacta
     2281 cttttcttat ggcctgacct caagtacgaa ttgctttacc ccattctgaa tttcgaatat
     2341 ataggagggc ttgttggtgg gtactccatc cttcatgtcc cactcataga gaagtccctg
     2401 aaagcctttt acattagcgc agtaatccgc aatcttcttg cgctcttcct tgagcttctt
     2461 tggatctcca gtaaccccgg tcttttcaat tgcttccttg atcatataga gtgcatcata
     2521 gtagttcggc gatagggaga agggcatctg gccattgtgg tctttcgcaa acgctgcttt
     2581 aaatttgtcc cagcgaggat tggatacttc caagttgagg tagttataga cctgaacacc
     2641 attcaaatct gctccgcctg tagagtatag ctgtgcatca tcagcactgg agaaaaccaa
     2701 gatatggttc atctttgtcc agccgcggct ctttagctcc ttgatgatct ttgcagcttt
     2761 ctctgcattg caaacgagaa ttattccatc aggtttctga tcgagagccc ttaccacaag
     2821 tgggccaaag gtgacagcgt cagtaggaac ctcgattgct ttcaattcct taatattaag
     2881 actggtgagc gctttgctat gtgcatctgc catgcctggc catggaccat agggctcgat
     2941 aaactgcaca acctgcttta gatcgctgaa ttgctttgcc cagccagcga ccaatggtgg
     3001 caatctatcc agtgtcggcg gaaaccatga aacagtccat gggaaaaact gtacggcata
     3061 ttcataggaa gtcgtcgcag ttatggacat catctgattt tctactgcta tcggcatggc
     3121 tgccaaaatt acaggctcgg gaacaggtcc catggctaca aggctatcct ttacaatgcg
     3181 tgccatctca actgaacctt tctgtggatc attgcctgta tctacaggaa tgaccctgac
     3241 cggtttccca gcaatgccac ctgcggcatt tatttcattg gctgcataat tcgctcccca
     3301 ttgtaagaat tcaccaattg ccgcaatagg gccggttaga caattgagaa atggaatcct
     3361 ccattcgttg accacaactt ttgtttggct aaagccaaaa ctcacagcca ccaaaattag
     3421 cagcaatgca acaatctgcc ttttcattgc tcttcctcct tactatatat ttcctttgct
     3481 cgcttgcgcg aaaaggataa acccaaggaa tgaacgaatt tcctggtaat gaagccttct
     3541 ggtttcaatt ccagaacaag aataagaagc aatccatata ttactatacg ccaagattgc
     3601 acttctgtgg ggaaaaataa gggcaagcca taaagaattg gagctgtaat aagcggaccc
     3661 cactgagtgg aatatccgcc cacgaaaaga accgtcataa aaatgcctat caaggcaaaa
     3721 ccgaaaaaat ccaggtgaaa gctcctatag ataaagccat atagaacgcc cacaccgcct
     3781 gcaagcgtac agctgaacgt ttgaaccagc ataccaagct ttttgatatc tattccaaga
     3841 gaggaagcca attctttgtc tacaaagacc gcagaagctg ctcgtccaat gctggaatga
     3901 tcgaatcgat agataagaaa gccacaaaga ataacaagcc catataagat acataggatt
     3961 atccaccgat gcgtccccgg gttgccacct atgttgggaa gaccaaacat tcccattgtt
     4021 ccgccgatta ttggcaaatt ctccacaatt gttcttgtaa tataaataga agtaaatcct
     4081 acgataacaa cggaaaatgt tggggcatca ccgatgaata gagatacaat aaaacctata
     4141 atacccccta ctgcaaagcc tattagaaga gcccatataa agggaacatg agcatgcaaa
     4201 accaaatatc cggcaagata cccagaaatc atcatgtttg cgatggtaat aaaatgcagc
     4261 tgcgtgaccc tatatggcac atatagagcc cagctcatca atatattcac tgctgtgatc
     4321 gaaagaaagt aatagagcga aagtggcatg ctctcctcct ataccttaac gccaaacaag
     4381 cctctcggct tgactagaat aattacaagc atgactacaa atgcaattgc attcgaccaa
     4441 ttccccgaga catatccctg gacaagtgct tcaagaatac cgagagctaa tcctacaact
     4501 aggcctccaa ccagattgcc aaggcctgcg actatagata ctgccaagac cttatgcgaa
     4561 acctgctctc ccaaacccgg agatgcagaa tggaaaagca ttatcagtag caccgctgtc
     4621 agcccgccca aagcccccga gagagcataa ctttgaaatc ccgtctttac caaaggaatg
     4681 ccgacaagtt tcgcaccggc tgggtcttct gcaatagctc tgaaagctct tcccattttg
     4741 gtcttatata gcagaataaa gaaaccaact acaacgatga ttccgatgga taatgctaag
     4801 acctgaccgg gcagaatgct aatcaacccg accctgaaca aaggcttact gccagtaatt
     4861 tcttcaggaa attggatcgg gagtccatta ttgaaggcat gcgaaagaac atcggtaata
     4921 atccatccta tggccattga tagaaccatt gttgcgttta tatcgacttc ttctctgttc
     4981 ttcatgattt tttgaaaaag cggcgcactg ataatgttaa ataatattgc agaagagact
     5041 ccagctagga ttccaaaaaa tacattatta ttggttatct tgagtaccag ccagataatg
     5101 tacatacaaa acacaacaac ttgaggatag gaaaaatgaa tgatcatcgc tacaagaagt
     5161 agaagattaa aagctgttgc taaaagcata tatacgcttc caatggaaag accattcaat
     5221 atttgcgcaa taaaaacttg catattcatg ctcctagata ggctgctttt acacctggat
     5281 catttaagag ccgagaagaa ggacctgaaa gagaaatggt acctgtttca aggacatatc
     5341 ctctgtgagc acaattgaga gctttgaatg cattctgctc agccaataac accgtatatc
     5401 cttctctgtt taaggtgacg agaatgtcaa aaatactatt gactatgata ggtgccagcc
     5461 caagcgatgg ctcatctatc attatcaatt ttggatctga catcagagct ctcgcaattg
     5521 caagcatctg ccgctcacct cccgaaagtg tccttgctat ttgatctttt cttttgtaca
     5581 aaaaaggaaa ccattcataa acacgcgcaa gattcttatc tttgtttgct aagttatgat
     5641 gggctccaag caatagattg tccaataccg acatcgaggc gaatactcct cttccttcgg
     5701 gcaccaaaca aagaccagcc ctaacatttc tatctacaga ggccttatca atcctttttc
     5761 cttcaaaaac aactttacct gaactcggtg tttttacacc aagtatagat gacaggagtg
     5821 tagttttccc cgctccatta gctccaatca aaactacgat ttcctgagga tatacttcta
     5881 tggaaatttt cttcagagct tgtatatgtc catatgatac tgaaatttcc tcgacagtga
     5941 gcatcggagc tttttctccg ctaaccaagg tgctattttg ttccaaggta tgcctccaaa
     6001 actcgaggat ccttttgaat attctctggg atatcctcag cgattttttg tccaaagctg
     6061 agcacggtga caatttcgga tagattcatt agcatcctta cctcatggct cactaccacg
     6121 attgttatgc ccttttttcg tattttcctg atgatctctt ccattttttc gatttcaagc
     6181 gatcccattc ccgatgccgg ctcatcgagc aacagcaatt gcggttcatt tataagagcg
     6241 cgtcctattt ggacaagctg tcgttctgtc cacactagat cgctagccca tctatgaatt
     6301 gaatcaacaa ttccaacaaa ttcggctatc ctttcagcct tttctttaat ttccttttct
     6361 ctcttggatt gagtaaaagg caaccgaatc attgtatcct ttagggattt ggataaaggc
     6421 tcttgaaaac cgcacattat attctcgagc actgtcagat tcgaaaccaa tttgccggct
     6481 tgaaatgtcc gatggatacc tttattcgca attatatttg gctttaggcc agtaatgtct
     6541 tcgcccttga aaatgacctt tccttcatta ggcttaagta tccctgttat gcaattgaaa
     6601 agagtggtct ttccagagcc atttggtccg attattccat gaatctctcc ctctttgatc
     6661 tcaatgtcca ctttatctaa tgcttgcaaa gcgccgaaac tcttggatag gccacttatc
     6721 ttcagcataa agaatccttt aaaataaaga tttctaggga ttatcagagg attttgaagc
     6781 aagaaatcgc gaagcagtca atgcatagat cttggcgcat tcatatgcct catctatctc
     6841 aacatattca tcgatctgat gaggtatatg ccgaggcccc ggaccattga cgatacaagg
     6901 gatgtttttc caggcactca ggaatgtccc gtcggttgca ccagggactc cattccatat
     6961 cggatctttt cctgtaa
//
LOCUS       DAOMZL010000082         6971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_10167_length_6971_cov_11.804078, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000082 DAOMZL010000000
VERSION     DAOMZL010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6971
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10167_length_6971_cov_11.804078"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..920)
                     /locus_tag="PLQ30_06745"
     CDS             complement(183..920)
                     /locus_tag="PLQ30_06745"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPW02179.1"
                     /translation="MERRFHQVIWAAAIMAVALVVMAVIVVNGFTKVRAAQDTISVTG
                     SAKEPVESDISVWNFSVNIRGNSLQAVYADLKTAIPKIDAFLATQGIPAEEIVASAIN
                     AGSNIYFDEDQQGRRIQHVTFFANRSYKVTSTDIDRLERVSRSAGDLIAQGVALDSVS
                     LEYMYTKLADLRTNLLERATEDAKKRAEAIASSSGAKIGAIKSARMGVFQITPRYSTM
                     VDDYGINDTSSRLKDVTAVVGAEFRLK"
     gene            complement(1272..2117)
                     /locus_tag="PLQ30_06750"
     CDS             complement(1272..2117)
                     /locus_tag="PLQ30_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPW02180.1"
                     /translation="MIKSQDMNHPYKLDISKTIVYGLMYVLAIIYLIPLIWMLRTAFI
                     HPDMAIDLFSLSWPTLANLKRVLLAAPFGQYYLNTIIMVVATLLVQFVLITMAGYAFA
                     RLDFWGKNLLFMLFLMQLMITPDVLIMPNYSFMGQIGLIDTKLGVILPFFASGMGTFL
                     MRQTIKTIPYELEEAAKMDGCRLPRMLLQIYVPLLKPVYIAFGLISASYQWNNFLWPL
                     VMINSVNKRPLTVGLAIFAMSFETGAQWSDVCAATLIVIAPLLVLFLFFQRQFIESFA
                     QSGIK"
     gene            complement(2148..3095)
                     /locus_tag="PLQ30_06755"
     CDS             complement(2148..3095)
                     /locus_tag="PLQ30_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPW02181.1"
                     /translation="MKNDIVHISDRPPAIKRYQSWIEEMDSNKKKKLKTSLLAWGLIL
                     PGFIFLTLFTLYPIAKSLYLSFFRIDLSVSTPEFIGLKNYTDLLKDDVFKQAFMNNIL
                     IALFTIPTSIALAVAMAIFANKVRVGKSLIRVAYFYPTILPMVAVANIWLFIYTPIYG
                     IIGYLDPSLRILGNPDTALWAIIIMLIWKQAGYVMVFYLSGLQGISRELYEAAELDGA
                     KPITIFHKITWPLLKPTTIYVAIISLTNAYKVVDHLYIMTKGGPGNATNMLLFYIYQV
                     GFDFWDTGKADTMTVVLIAMLLLITAIWFFSQDKRTFYG"
     gene            complement(3227..4543)
                     /locus_tag="PLQ30_06760"
     CDS             complement(3227..4543)
                     /locus_tag="PLQ30_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW02182.1"
                     /translation="MKKASLFLVLALSFIVPVIKNGALIYAAEKTINLDLYFPVAVGG
                     GPDKLISALCNQFSKENPNIKVNPVYSGSYADTRTKVQAAIKARKPPAVALMFSIDLF
                     SLLSMDAILDYESLCTTKDDREWLSGFYDGFMKNSRTGGKTYGIPWQRSTIILYYNKD
                     AFREAGLDPNKPPSTWAELEEYAKKLTKVENGITTRYGIEIPSDKAGYAYWMLQTFCT
                     QQNGFNLMNEEGDEVYFNDPRTIKGITFWKSLSDKGYQPRGTTAWATTPTDFLEQRAA
                     MIYHTTGNLTNIRKNAKFDFGVAMLPADLSRGSPTGGGNFYLFKGVPREQTEAAFKFI
                     KWMTGDPNRVAQWSIDTGYVATRPEAYETQIMKDYAAKFPEALVARDQLKYGYAEFSV
                     YDQARVQKILDDAIELIMTGQKDPVTALNEAQAQADQILKQYRKQK"
     gene            4984..5181
                     /locus_tag="PLQ30_06765"
     CDS             4984..5181
                     /locus_tag="PLQ30_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02183.1"
                     /translation="MGYKGPQPKSGNHYALVQRIAILIHENHYSPYVVLNKLDEEGSW
                     PSGLRICEKTLYNGVEFSLVN"
     gene            5545..6612
                     /locus_tag="PLQ30_06770"
     CDS             5545..6612
                     /locus_tag="PLQ30_06770"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HPW02184.1"
                     /translation="MGIFLVTFGGTQQALGQQGSIPQVQRSSQQPQLQQQTTVLPASN
                     DTDDISLPALLDHMLDNHYLPSVQVALGSFTYADTQLPTPFARWFEDELRLALGRTSK
                     MKLFDKQVAAAMDPAIRAQYADFFGQDRADSILYGKYARDGQGVLVTLSLTDLASGAL
                     ISEIRYPVKKELVPTDVVVEPSIKTQQTAVSLSQLIPASGSQASGGQSSGSQSYAAPD
                     FLLSLSTDRGGGAVYRNGERLKLYLTSNKDAYLKIYHVDVNGVAQLIWPNRFGGSGKI
                     MAGEALQFPGPGDKFQYILGPPYGTEYIKAVASTKAFATMEADFSDLQGSAAAAITRG
                     LSVVSSDATRSEALAVYEIVP"
BASE COUNT         1965 a         1537 c         1464 g         2005 t
ORIGIN      
        1 agattctaag ccttacaaag aaagccgtac gctcggtctt gctaagagat tgatattcgc
       61 tgtttcttct attaattttg tcgccttgat aatgagcccg ctcatcaaaa tccacataga
      121 agaatggagc gcctcatagc tttgtattat ataagatgcc tactaatgaa cgccatttaa
      181 cgtcatttaa gcctgaattc ggcgccgaca actgcggtga catcttttag acggcttgaa
      241 gtatcgttaa tcccatagtc atccaccatg gtcgagtagc gtggtgtgat ctggaaaacg
      301 cccatgcgcg cgcttttgat tgctccaatc ttggcgcctg atgaagaagc aatagcttca
      361 gctcgctttt ttgcgtcctc tgtggcgcgc tccagcagat tagtccgtaa atcagctaac
      421 ttggtataca tatattcgag agagacagag tctagcgcaa cgccctgggc gataaggtcg
      481 ccggcactgc gcgatacgcg ctccaatctg tctatatctg tcgatgtcac tttgtaactg
      541 cggttggcaa agaaggtaac atgctgtatg cgacggcctt gctgatcttc gtcaaaatag
      601 atattgctgc cagcgtttat ggcactggcg acaatttctt cggctggtat gccttgtgtg
      661 gccaaaaatg catcgatctt ggggatggct gtctttaaat ccgcatatac agcctgaagg
      721 ctattcccgc ggatgttgac ggaaaagttc caaacggaaa tgtccgattc gactggctct
      781 tttgcgcttc ctgttacgct gattgtgtct tgtgcagcgc gaaccttcgt aaaaccattg
      841 actacaatga cagccattac aactaaagcg actgccataa tagctgcagc ccaaattact
      901 tgatggaatc ttctctccat aatcttctac ccccacttag agcaaaacct agcagtaatc
      961 ccaggtgtta ttctattatt ggctttagcg cacaactcct gggtgatttc atctctttgc
     1021 tccagcatat aaggccacat caattctatc tctattctct tggatcattc aatcaggcgg
     1081 actgaaagat ctcgatatct aatgatgcga tgatttctcc ttgatatttg atggctttcc
     1141 cagcagttgc ttaatcttgc tctcatcaca aatgtccatg ggcaaatttc atcgacgatt
     1201 ataagaacga tcttttccta tcgtggaaat gctccgcagt attctctctt tatccctact
     1261 ttatgtattt cttatttgat gccagattga gcaaagctct caataaattg tctctgaaag
     1321 aaaagaaaaa gaacaagcaa aggcgctatg acaatcaggg ttgccgcaca gacatcgctc
     1381 cattgtgcac ctgtttcaaa ggacatggca aaaatcgcca atccaactgt caatggccgc
     1441 ttatttaccg aatttatcat gaccaatggc cataggaaat tgttccattg gtatgatgct
     1501 gaaatgagac cgaatgcaat atatactggt ttgagcaaag ggacataaat ctgtaagagc
     1561 attcgaggga gcctgcatcc atccattttt gctgcttctt ctaattcata aggaatagtt
     1621 tttatcgtct gtctcatcaa gaaagtgccc attccagatg cgaaaaaagg aagaattacg
     1681 cccagtttag tatcgattag accaatttgc cccataaaac tatagtttgg cattatgaga
     1741 acatcaggcg taatcatcaa ttgcataagg aacagcataa acaatagatt tttgccccag
     1801 aaatcaagtc ttgcgaatgc atatccagcc atagtaataa gaacgaattg cactaaaaga
     1861 gtagcgacaa ccatgattat tgtgtttaaa taatactgtc cgaaaggagc agcaagaagg
     1921 acccttttta aatttgccag tgttggccaa cttaaggaaa agagatcgat tgccatatca
     1981 ggatgtatga atgctgttct aagcatccat atcaatggga ttaggtatat aatagccagg
     2041 acatacatga gcccatagac aatggtcttg gatatatcta gtttataagg atgattcata
     2101 tcctgcgact ttatcataaa gcctccataa tagatgactc catagcttta gccatagaat
     2161 gttcttttat cctgggaaaa aaaccagatt gccgttatca acagaagcat agcaatgagt
     2221 actaccgtca tcgtatccgc tttccctgta tcccaaaagt cgaatcccac ctgataaatg
     2281 tagaatagca acatatttgt ggcattgccg ggtccaccct ttgtcataat atatagatga
     2341 tcaaccacct tatatgcatt tgtcaggctt attattgcta cataaatggt agtcggtttg
     2401 agcaatggcc aggtaatttt gtggaatata gtaattggtt ttgccccatc gagctctgcc
     2461 gcttcatata attctctcga aattccttga aggcctgata gatagaaaac cataacataa
     2521 ccagcttgct tccatatgag cataataatg attgcccata atgcggtatc tggatttcca
     2581 agaatgcgca aactgggatc gagatatcca ataatgccat aaataggtgt ataaataaaa
     2641 agccaaatat tggctacagc aaccatgggt agaattgtag ggtagaaata agctacccgt
     2701 atcaatgatt tcccaactct caccttattt gcaaagatag ccatggctac agccaatgct
     2761 atacttgtag gaattgtgaa taaagcaatt aaaatattat tcatgaatgc ttgcttgaat
     2821 acatcatctt ttagcaaatc agtatagttc ttgaggccga tgaattctgg ggttgataca
     2881 gaaagatcaa tcctgaaaaa gctcaaatat agactttttg ctatagggta gagagtaaaa
     2941 agcgtaagaa aaataaaacc aggcaggatc aagccccagg caagaaggct cgtctttaat
     3001 ttttttttct tattgctatc catttcctca atccaacttt ggtatctctt tattgccggt
     3061 ggcctatctg atatatggac aatatcgttt ttcataggct tatcctttta caagttagcg
     3121 caatacaatc tgcaacaaga aatcaaacct aataaaagca gagcttccaa cataatagta
     3181 atagtcaatg gtggaagctc tttttttaaa gcatgttatt ggtatcctat ttttgcttgc
     3241 ggtattgctt gagtatctgg tctgcttgtg cttgagcctc atttagagct gtgacaggat
     3301 ccttttgacc agtcattatc aattcgattg catcatcaag gattttttga actcttgctt
     3361 gatcataaac cgaaaattct gcatatccat atttaagctg atctctagca acaagcgctt
     3421 caggaaattt cgcagcatag tctttcatta tctgtgtttc ataggcctcg ggtcttgttg
     3481 ccacatatcc cgtgtcgata ctccattggg ctactctatt aggatcacct gtcatccatt
     3541 taataaattt aaaagctgcc tcagtctgtt ctctaggtac tcccttgaat aagtagaaat
     3601 tacctcctcc tgttggggat cctctagata aatctgcagg aagcatggct actccgaagt
     3661 caaactttgc attcttgcgg atattggtaa gattgcctgt tgtatgatag atcattgcag
     3721 ctctctgttc taggaagtct gttggagtag tcgcccaggc tgttgtaccg cgtggctggt
     3781 atcctttatc tgatagactc ttccagaaag tgatgccttt gatagtccgc ggatcattga
     3841 aatagacttc atcaccttct tcattcatta gattaaatcc gttctgttgc gtgcagaatg
     3901 tctgtaacat ccaatatgca tagccagctt tatctgaagg tatttcaatg ccatatcgag
     3961 ttgttattcc attctcaacc tttgtcaatt ttttcgcata ttcctcaagt tctgcccaag
     4021 ttgaaggcgg cttatttgga tcaaggcctg cttctctgaa tgcatctttg ttgtaataca
     4081 agatgatagt acttctttgc catggaatcc catacgtttt accaccagtc cgggaattct
     4141 tcataaaacc atcatagaat cccgatagcc attctctgtc atcttttgtg gtgcataggc
     4201 tttcgtaatc gagtattgca tccattgaca aaagtgagaa caagtctatg gaaaacatta
     4261 gagctacagc cgggggctta cgagctttta tggccgcctg tactttagtc ctcgtgtctg
     4321 cataactccc agaatagaca gggttaacct taatgttagg gttttctttg ctgaattggt
     4381 tgcacaatgc actgatgagc ttatcgggtc cgcctccaac agcaacggga aaatacaaat
     4441 ctaggttgat agtcttttca gcggcgtata tcagtgcccc gttcttgata actggaacaa
     4501 taaaagagag cgctaatact aagaataggc tagctttttt catttggtgc ctcctttagc
     4561 aatatcagtc gaaaactgac ggaataaaat atatctaatc ggatcaaccg atagattata
     4621 caaacataac ttgagcccgg tttcagatat agcagatctt taggccgctt tattatcatt
     4681 ttgtaatcca gactttaaag ataccctggt ctcaaaaata ataatttcat taattcctag
     4741 catgtttatt ctttgcggtc aagcgatata ttacgatgtt tggatttcag gtgagcagta
     4801 acataaaata tccagctaaa aggggaagaa tcaagagaga aaaaaagtca catggaagtc
     4861 gtgccatttt tggttttgca aaaaatcaaa atatagacgg agattccatg ggacgcacac
     4921 tgcttactct ttgtacgaat agaatacaat gcggagcatt cgcagctgga tgcagaagcc
     4981 aagatggggt acaaggggcc gcagccgaag tcgggtaatc actatgcact cgtacagaga
     5041 atcgccatct tgattcatga gaatcactat agtccctatg tggtgctcaa taagctcgat
     5101 gaagaaggct cctggccttc gggtttaagg atctgtgaga agacactcta taatggtgtt
     5161 gaattctccc ttgtcaacta gctttagcac tcgatcttca cgaacgacac acggacaacg
     5221 ctctattttg ctcatcccta ttgttcttcg gagcgggaga ccagtgagaa ccacaacggc
     5281 attattcgaa ggtttctgcc caaaggaacc aatttcagcc gtgtgaacga tacgcggatt
     5341 agagatattc aagactggat gaatacctat ccacgaaaga tccttggtgg ttttacccca
     5401 ttgcatttta cagcagacct gcaactacca aattgcgaac ttaattttac actataataa
     5461 agtcgtgcta tactaattca attattttga tggacaaaga ctatgcgagg caaagcgtcg
     5521 ttccgaattg gcaggtttct ttttttgggg attttcttgg taacctttgg aggaactcag
     5581 caagctctag gccagcaagg ctccatccct caggtgcagc ggtcgtcaca acagccgcag
     5641 ctgcaacagc aaaccacagt gctgcccgcc agtaatgata cagatgatat ttccctccct
     5701 gcgctgcttg atcacatgct ggacaaccat tacctgcctt cagtgcaagt tgcattgggc
     5761 agttttactt atgccgatac gcagctccct acgccattcg cgcgctggtt tgaggacgaa
     5821 ctgcggctcg cgcttggtcg cacctcaaag atgaagctat ttgataaaca ggttgcagca
     5881 gcgatggatc cagcgatccg tgcgcaatat gcagacttct tcgggcagga tcgcgcggat
     5941 tcgattctct atggaaagta cgcgagggac ggacagggtg tgttagtcac gctttcgttg
     6001 acggatcttg cgagcggcgc cctgataagc gaaataaggt acccggtgaa aaaagagttg
     6061 gtccccaccg acgttgtggt tgagccttcc atcaaaacgc aacagacagc agtcagcttg
     6121 agccaactta ttcccgcctc tggaagccag gcttcgggtg gccaaagctc tggcagtcaa
     6181 tcctacgcag cgcccgattt tttactcagc ctgtctacag accgcggagg aggggcagtc
     6241 tatcgcaatg gcgagaggct caaactctac ctgacctcga ataaggatgc ctaccttaag
     6301 atctatcatg tcgatgtcaa tggtgtagct cagctaatct ggccaaaccg attcggagga
     6361 tcaggcaaaa taatggcagg tgaagcgctt caatttcctg ggcctggtga caagttccaa
     6421 tatatattag gccctcctta cgggacagaa tacataaaag cggtggcttc gaccaaagct
     6481 tttgccacca tggaagcgga cttttctgac cttcagggga gcgctgcggc cgctattacg
     6541 cgtggtctct ccgtagtttc gagcgatgcg acgcgctccg aagcccttgc ggtctatgaa
     6601 attgtgcctt gatttcgatc tcttagccac caaaccccat taagagctgt gagagcgcaa
     6661 ttccgaggta atttccgatc gcatacccaa tgatccccgt cgttatgcca ggcaattaag
     6721 tggatatttc actttacagg agaccaaact cgtaagctgg cctcctttat gctttatcct
     6781 tccccagagc tgaagtacaa tagaaaaatg atgaatcgag aggcctagaa agacccctca
     6841 aagcgcaata tgcaataaga gaatgtaagg ataagttgcc atgaaagcaa tatcaaaatg
     6901 aggtatttcg atgcttgatg tattccagtt tcgcaatgaa atcatcgatg aatatgaaaa
     6961 gttttcccga t
//
LOCUS       DAOMZL010000083         6955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_10184_length_6955_cov_16.443768, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000083 DAOMZL010000000
VERSION     DAOMZL010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6955
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10184_length_6955_cov_16.443768"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PLQ30_06775"
     CDS             <1..566
                     /locus_tag="PLQ30_06775"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HPW02185.1"
                     /translation="PKGGDSFEGSESRNLNRVYPGKPDGTLTEQLAWAVMTLLKKEKV
                     DIAFDLHEAGPESRLANMIVANPKNLDLGALAVINLELEGINMKLEPSSDVFHGLSHR
                     EWGDETSASAFLIETPNPAQATDKGNPVEDPKHPLEERVAIHLATIEAILDAWNSDCL
                     PDKHIEFAMFPSWQAIKARGVGESLNW"
     gene            595..2265
                     /gene="ggt"
                     /locus_tag="PLQ30_06780"
     CDS             595..2265
                     /gene="ggt"
                     /locus_tag="PLQ30_06780"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPW02186.1"
                     /translation="MAQKNKSVGLGMKHGVRKFLLFLLAAFVFALPIFAADTINAEIT
                     ARNGMVASAQPLASAAGLEILMAGGNAIDAAIAAAFALGVVEPNATGLGGEGMMVIYL
                     AENKTTIAIDYRSMAPLADMSKIKFGSEGHVAVAVPGTVAGLCTALKDYGTKSLAEVM
                     APAIRYARNGFIVSETLAQTIADRFDPISRNEALLQILAPEGLPLQTGDIFKNPDLAV
                     TLEKIAAGGPDVFYKGDIADAIAQDMAKNGGFITKADLAAYRAIKREPVRGTYRGYEI
                     VSAPPPVGGISVIEMLNMLECFDIASEQPLSPRNIHIMAEVMKRGFADNSAYVGDPDF
                     FQIPVTGLLDKEYARSRVQEIDLNKVTTSIKAGTPDEHPSTTHLSVVDRHGNMVALTQ
                     TISGFWGACVAVPGTGIILNNEMQNWSSRGPNSYAPGKRMRTTIAPTIIAKDGNPFVT
                     MGTPGAGRIISTMVILTVNLIDFDMGVQEAIESPRFYARDTEKLLSLESRIPKETQEW
                     LKSIGYSIKEYPPFDLFFGGAQAILVDPKTGIMHGGADPRRDGAVFGF"
     gene            2328..2870
                     /locus_tag="PLQ30_06785"
     CDS             2328..2870
                     /locus_tag="PLQ30_06785"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HPW02187.1"
                     /translation="MLLGILPIFAQYSVPSTLVASFERPGFITSAGQTSDAAIVKVLA
                     NTRLKLGLDYDIAAKPETIANAKTLVLVLGVSNKGLGAAGLSIEQEIERVQSIVAEAL
                     KNNTRIISIHTGGTARRGEASNQVIELCVPASDVVIVVEGGNNDGFFTSLCEENNVFL
                     VQVPSIAEAGNVLKSLFEAQ"
     gene            2867..3961
                     /locus_tag="PLQ30_06790"
     CDS             2867..3961
                     /locus_tag="PLQ30_06790"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HPW02188.1"
                     /translation="MRKFFLAWFVLLGLFVSQAQTEDGVAFVMSEVKAGKGVEKVLSL
                     SAFLSSLEGTAFDTPVFFLGDSPALHVKNAPERSISEETTEKQDSSSTDYTVAIVAGA
                     HANEIAGSLAAYWIIEHCRVSGGKLIVVPRANASAATWSTSKVAAPILHYGARLSNPA
                     HEKGPDPGFFIPFDASESLPSLSGQEVRNLNRQYPGDPTGNYTSQIAYAITRLLVQNR
                     VRTALDLHEASPGSSLAWSIITRPEYLDVAALAVLQIEEETGKSFHLESSRDEFAGYS
                     HWEWGKLGIRAFLVETLNPAQPSDDPTIDQIFNDKAPLAERVFIHLCTVKALVASAYE
                     ELGDLVALNIEGLPSSKEEVQKWLLGIEKN"
     gene            4005..4490
                     /locus_tag="PLQ30_06795"
     CDS             4005..4490
                     /locus_tag="PLQ30_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02189.1"
                     /translation="MNTVYLSKDLAMERVSSFLSRWKNSTDQDIDFDLAKSSEIIEDA
                     SELLRKVPREVQKIGCYCLASQRKVVVWLLTDESGHGSGDEERGQDFLQIVLQKPPEF
                     GSAPVEIKYNVMSNSADRNNNVCLCGIEISYGNILILSPPATGEMVISISRIELAKEL
                     S"
     gene            4563..6296
                     /gene="pgsB"
                     /locus_tag="PLQ30_06800"
     CDS             4563..6296
                     /gene="pgsB"
                     /locus_tag="PLQ30_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HPW02190.1"
                     /translation="MISFIVILSIGLAIAAIVEYKDHCARLKAVPIRILVNGTRGKST
                     VTRLIAAGLAAAGLRVYAKTTGSAARIILPDLSEREIRKHGTAKRRPSIMEHKWFVRE
                     AHRAGAQAIVAECMAIRPETIGVLETRLAHSTIGVITNVRLDHLDTMGTELESIAEAL
                     GESIPDRGKAFVGSEGMGEYIQAVFSRMAAQRGSELIFVPSAEETQALHRDFLYPVHA
                     QNLALALAVCDACGVSCQIALRGMQSAKPDPGVRPTLQTTWRRHKVRVINAFAANDPQ
                     STLEMWHEERGLRYSSDLCYPSDMRNLNELDSGYGLQKNETHEILSEIKEVNSAEQTF
                     RVLVFNHREDRAWRALQIGEIAAGINADAILVYGMNQRLAEKVVYRFYRRLGVGKLTE
                     PKTQQAGLVPELAQSELTTAALHFASAVSHLQLLPWLQRVSPASPQAQLEPQSAQSQT
                     QTPSKPIKYPIVRSMRQASFDALCETIESLGGFKESPLKTYVEILCAGNIKGPGMDLS
                     DSLEALLLHRPDVARSAISTEGAQSISSPEGEQSFDSTGVAQPVTSAEGAHSVVSPEK
                     SQSANSTGEKR"
     gene            6293..6724
                     /gene="pgsC"
                     /locus_tag="PLQ30_06805"
     CDS             6293..6724
                     /gene="pgsC"
                     /locus_tag="PLQ30_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HPW02191.1"
                     /translation="MTSISIIVSIVLGFFFGEMTGWLTGGLVAPGYLALYIDQPLRIL
                     MTYMAALLAFFLIKLLAKITILFGRRRFMAFILAGICMGALLDAVVRLIPPSGTDLRA
                     IGYVVPGLIANDIWKQGLWRTLLGSLVITLATRVVLLLILA"
     gene            6733..>6955
                     /locus_tag="PLQ30_06810"
     CDS             6733..>6955
                     /locus_tag="PLQ30_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02192.1"
                     /translation="MKSSFAKHRQNRAISIRVFIAVISLALGLVCTELLARNEPHPVA
                     KEMKMAAQRMAQAEAVVAKRRLAMGLPMDP"
BASE COUNT         1822 a         1541 c         1786 g         1806 t
ORIGIN      
        1 atccaaaagg aggtgattct ttcgagggct ccgagtctcg caatctcaac cgtgtttatc
       61 ctggtaagcc tgatggaact ctaacagaac aacttgcatg ggcggttatg actttgctca
      121 aaaaggagaa agtggacata gccttcgatt tgcatgaagc tggaccagaa tcacgccttg
      181 ccaatatgat tgtggcaaat ccgaagaacc tggatcttgg tgccttggcg gtgatcaacc
      241 tggaattgga agggattaat atgaaactgg aaccttcgag cgacgtgttc catgggcttt
      301 ctcatagaga atggggagac gagacgagtg catctgcttt tcttattgag actccgaatc
      361 cagcccaagc gaccgataaa gggaatccgg tagaagatcc aaagcatcct ctcgaagagc
      421 gtgtggccat acatttggca acaattgaag caattctgga tgcatggaat tccgattgtt
      481 tgccagacaa gcatatagaa tttgcaatgt ttcctagctg gcaggctatc aaggcgcggg
      541 gtgttggcga aagtctaaac tggtaaacta attgatattg aggaggtttt tgatatggct
      601 cagaagaaca agagtgttgg cttaggaatg aagcatggtg taagaaaatt cttattgttt
      661 ttattggctg ctttcgtctt tgctctgccc atatttgctg ctgatacaat taacgctgag
      721 attactgcac gcaatggcat ggtggcttcc gctcagccgc tcgcttccgc tgccggtctt
      781 gaaattctaa tggctggtgg caatgcaata gacgcagcca ttgcggcagc ctttgccctt
      841 ggggttgtag aacctaatgc aacaggtctt ggcggcgaag gcatgatggt catatatctc
      901 gcagagaata agacaactat tgcaatcgat taccgttcaa tggcgcctct ggcggatatg
      961 tcgaaaatca aatttggcag tgaaggtcat gtggcggtag ccgtcccagg tacggtagcg
     1021 ggtctttgca ccgcactcaa ggattatgga acaaaaagtc tggctgaggt catggcacct
     1081 gctattcgct atgctcgcaa tggatttata gtgagcgaga cgctagcgca gacaattgca
     1141 gatcggttcg atcctatctc gagaaacgaa gcgcttttac agatccttgc gcctgaaggc
     1201 ctgcctttac agactggcga tatattcaaa aatccagact tagccgttac tctcgaaaaa
     1261 atcgctgcag gagggccaga cgttttctac aaaggagata tagccgacgc tattgcacag
     1321 gatatggcga aaaacggcgg ttttattaca aaagcagacc tggctgcgta tcgagcgatc
     1381 aagcgtgagc ctgtgcgtgg tacatatcgt ggttatgaaa tagtatcggc gcctccgccc
     1441 gtaggtggaa tttccgtaat agagatgctc aacatgctcg aatgttttga tatagcatcc
     1501 gagcagcccc tttctcccag gaatattcac attatggctg aagtcatgaa acgaggattt
     1561 gcagataatt ctgcctatgt aggggatcct gattttttcc agatacctgt aacaggcctt
     1621 cttgataaag aatatgcgag aagtcgtgtc caagaaatcg atttgaataa agtcacgact
     1681 tcaataaagg ctggaacacc cgacgaacac ccgtccacaa cgcatctctc cgttgtcgat
     1741 cggcatggta acatggtggc gctcacgcaa accatttcgg gattttgggg agcatgtgtt
     1801 gcggtgcccg gaactgggat cattctcaac aatgaaatgc agaattggtc ttcgcgcggt
     1861 ccaaattcct atgcacccgg gaagcgaatg cgcactacta tcgcccctac gatcatagca
     1921 aaggatggga atccttttgt gacaatgggg acccccggag cgggaagaat aatttccact
     1981 atggtcattt tgacggtgaa tctcatagat ttcgatatgg gtgttcagga agcgatagaa
     2041 tcccctagat tctatgcgcg ggataccgaa aaactcctat ctttggaatc gagaattccc
     2101 aaagaaaccc aggaatggct caaatcgatc ggctatagca taaaggaata tccgcccttt
     2161 gacctctttt ttggcggagc acaggcaatt cttgtagatc cgaagacggg aatcatgcac
     2221 ggaggtgctg accctagacg cgatggtgca gtcttcggat tttgaaggtg tggcatatga
     2281 tttatagata ttctagtaca aagagatttc tcgcaatagt gtgttttatg cttttaggaa
     2341 tattgccgat ttttgcgcaa tattctgtcc catcgactct tgtggcgagt tttgagcgtc
     2401 caggatttat aacatcagct ggtcagactt ccgatgccgc aattgttaaa gtcctggcca
     2461 acacaaggct caagctcggt cttgattatg atattgcggc aaagcctgag actatagcaa
     2521 acgcaaagac gctagtgctc gttcttggtg tctcgaacaa aggtcttggt gccgcagggt
     2581 tgagcattga gcaggaaatt gagcgcgtgc aaagcatcgt tgctgaagcc ctcaaaaata
     2641 atacccgaat catttctata catacaggtg gaaccgcgcg ccgaggagaa gcctcgaatc
     2701 aggtaataga gctctgtgtc cctgcttctg atgtggtaat tgtcgtcgaa ggcggcaaca
     2761 atgacggttt ttttacttct ttgtgcgaag agaacaatgt cttccttgtt caagtccctt
     2821 cgatcgccga agcaggtaat gtgcttaaga gtctttttga agcgcaatga gaaagttctt
     2881 tcttgcttgg tttgttttgc tcggcctctt tgtttcgcaa gctcaaacag aggatggcgt
     2941 agcgtttgtt atgtcagaag tgaaggcagg gaaaggtgtc gaaaaggttc tttccctgtc
     3001 tgcttttttg tcgtcattag agggaactgc ttttgacaca cctgtttttt ttctcggaga
     3061 cagcccagca ctgcatgtga aaaacgcacc tgaaagatct atttcggaag aaactactga
     3121 aaaacaggat agttcctcga cagactatac ggttgctata gtagccggtg ctcatgcaaa
     3181 cgaaattgcg gggagtctag cggcatattg gataatcgag cattgcagag tgtctggcgg
     3241 caagctcatt gttgtgcctc gagcaaatgc ctcggctgca acatggtcga caagcaaagt
     3301 cgctgcaccc attcttcatt atggagctag gctttccaat cctgcccatg aaaagggccc
     3361 agatcctggt ttttttattc ccttcgatgc ttcggaatct ttgccttctt tatctggtca
     3421 ggaagtgcga aatctaaatc gtcaatatcc cggggatcca acgggcaact acacaagtca
     3481 aattgcgtat gctatcactc ggctactcgt gcagaaccgt gtgcgaacag cgctggatct
     3541 gcacgaagca agcccaggtt cttctcttgc gtggtctatc attacaaggc ctgaatatct
     3601 cgatgtggca gcgctcgccg tgcttcaaat agaagaggag actggaaaat cctttcacct
     3661 tgagagctca agagatgagt ttgcaggcta ttcgcattgg gaatggggaa agctaggtat
     3721 tcgagctttt cttgtggaaa cactgaaccc agcacagccg agcgatgatc ctaccatcga
     3781 tcaaatcttc aatgataagg cccctcttgc agagagggtt tttatacatc tctgcacagt
     3841 aaaggctctt gtggcttctg cttacgaaga gctgggagat ttagttgctt tgaatatcga
     3901 gggcttgcct tcgtcaaaag aagaagtaca aaaatggctg ctcggtatag aaaagaatta
     3961 aagcgctgta ttttgtgctc aatatcgctc aatatattca tagaatgaat acggtttatc
     4021 tctccaagga cctagccatg gagcgagttt catcgtttct ctcacgatgg aagaatagta
     4081 ccgaccaaga catagatttt gatctggcaa aaagttccga aatcatcgaa gacgcgtctg
     4141 agttattgag aaaagtgccc agagaggtgc aaaagatagg atgctattgc cttgcttccc
     4201 agaggaaagt cgtagtctgg ctcctaacag atgaaagcgg acatgggagc ggcgatgagg
     4261 aaagaggaca agattttctt caaattgtat tgcaaaaacc cccagaattt ggatcggcgc
     4321 cggtagaaat taagtacaat gtaatgagca attcagcaga ccgtaataat aacgtatgtc
     4381 tctgtggtat cgagatatcc tatgggaaca ttctaatatt gagtcctcct gcgaccggtg
     4441 agatggtcat ctctatatcg agaatcgagc tagccaaaga gctgtcctaa ggcgctaatc
     4501 ctatagaaca actaccttgt aacatgagac aatggagtca gagggagagc agatataaag
     4561 ccgtgatttc ttttattgtt atactaagta taggtttagc aatcgctgca atagtagaat
     4621 acaaagatca ttgtgcgcgc ctcaaagccg tgcccatacg cattcttgtc aatggcacgc
     4681 gcggcaagtc cactgtgaca agacttattg cggcagggct agctgccgca ggtttgcgag
     4741 tatatgcaaa gaccaccggc tctgcagccc gcattattct tccagatttg agcgagagag
     4801 agataagaaa acatggtact gcaaagaggc ggccttcgat aatggaacac aaatggtttg
     4861 tacgagaggc acatagggct ggggctcagg ctatagttgc agaatgcatg gcgattcgcc
     4921 cagagactat cggtgtgctc gagacgcggc ttgctcattc gacaatagga gtcataacaa
     4981 acgttcggct agatcatttg gatacaatgg ggacggagct tgaatcgata gccgaggctc
     5041 tcggggagtc tattccagat cgagggaagg cctttgtggg cagcgaaggt atgggcgaat
     5101 atatacaggc tgttttctcg cgtatggccg cgcagcgcgg ttctgagctt atcttcgtgc
     5161 ccagcgccga agaaacgcaa gcgctgcaca gagatttttt gtacccagtg catgcgcaga
     5221 acctggctct tgctcttgcg gtgtgcgacg cttgcggtgt atcgtgccaa attgcgctgc
     5281 gcggcatgca aagcgccaag ccagatccag gcgtgagacc tacgcttcaa acaacatggc
     5341 gaagacacaa ggttcgagtc atcaacgcct ttgctgcaaa cgaccctcag tctacgcttg
     5401 agatgtggca tgaggagcgt ggcctgcgtt attcgagcga cttgtgttac ccgagcgata
     5461 tgcgcaactt gaatgaatta gatagcggtt atggccttca gaagaatgag acacatgaaa
     5521 ttctctcgga aataaaagag gtcaacagcg cggagcagac tttccgcgtg cttgttttca
     5581 atcatcgaga agatcgtgct tggcgagccc ttcagattgg cgaaatcgct gcgggcataa
     5641 atgcggatgc aatccttgta tatggcatga atcaaaggct agccgaaaaa gttgtctatc
     5701 gattttatcg tcgactaggc gtaggaaagt tgacagagcc caaaactcag caagctgggc
     5761 ttgtaccaga gctagcgcag tcagagttaa caacagctgc gctgcatttt gcttcggccg
     5821 tatcgcattt gcagttattg ccatggttgc agcgagtaag tccagcgtca cctcaggcgc
     5881 agcttgagcc gcagtcagca cagtcgcaga ctcaaacccc cagtaagccc ataaaatacc
     5941 ccattgttcg ctcgatgaga caagcaagct tcgatgctct atgcgagaca attgaaagcc
     6001 tcggaggttt taaggaaagc ccattgaaga cctatgtgga aatactatgc gcaggtaaca
     6061 taaaaggacc ggggatggat ttgagtgatt ctctcgaagc tctattattg catcgaccag
     6121 acgttgcacg atcggctatt tcgaccgaag gggcgcaatc gatttcttca cctgaggggg
     6181 agcaatcgtt tgattcgact ggcgtagccc aaccagttac ctcagccgag ggggcacatt
     6241 cagttgtttc gcctgaaaaa tcgcaatctg ctaattcgac aggagaaaaa cgatgacaag
     6301 catatcgatt attgtttcaa tagtgctcgg atttttcttt ggcgaaatga caggatggct
     6361 tacaggcgga ctcgttgcac ccggatacct cgcactttat attgatcagc ccttgcgaat
     6421 actcatgaca tacatggcag ctcttttggc cttctttttg attaagttgc ttgcaaagat
     6481 caccatctta ttcggaagaa ggcgttttat ggccttcatt ctagccggca tttgtatggg
     6541 tgctttgctg gatgctgttg taaggctaat tcctccttca ggcaccgatc tgcgagcgat
     6601 tggctatgtg gttcctggcc ttattgcaaa tgatatatgg aagcagggtc tttggaggac
     6661 tttgcttggc agccttgtaa ttactcttgc aacgcgtgtt gtgctgctct tgatcctggc
     6721 ttgagtcatt ccatgaaaag ctcgtttgca aaacacaggc agaatcgcgc gatttctatt
     6781 cgcgttttta tcgcggtcat ttcgctcgcg cttggtcttg tctgcacaga gttgctcgcg
     6841 cgcaacgaac cccaccctgt tgccaaagag atgaaaatgg cagcccaacg aatggctcag
     6901 gcagaagctg tagtcgcgaa aaggcgcctt gctatgggat tgcccatgga tcctg
//
LOCUS       DAOMZL010000084         6872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_10344_length_6872_cov_13.184979, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000084 DAOMZL010000000
VERSION     DAOMZL010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10344_length_6872_cov_13.184979"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PLQ30_06815"
     CDS             complement(<1..840)
                     /locus_tag="PLQ30_06815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW02193.1"
                     /translation="MSEQSISVIVPTYKRAHLLSLTLPTYLQPETSELIVVDDCSPDT
                     TREVVESLAADDPRIRYIRTDANRKQPHAKNLGISNARGEYLYFGDDDSVLLPGSLER
                     LITTLHDYGALIVGACAPYITRLSKDKDFSSIFEEILGEIEQSERLQKTKINKRLIDP
                     VSLEARFNIRTKEPVEVPFVQASFLVSRELAEKICFDEHYFGNCYREETDFLIRAHNT
                     GAKIIYEPRAIQANLPRSIAIGGAHDGSSNFLIRKLSYFKSALGNNWYFLTKNKEALQ
                     KAFG"
     gene            complement(833..>1525)
                     /locus_tag="PLQ30_06820"
     CDS             complement(833..>1525)
                     /locus_tag="PLQ30_06820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPW02194.1"
                     /translation="RARIIVAVSEHSQRDWQRYYHLSSGKFEHLPNTVDTKHLQPTSE
                     LNEQESSKQSKRVLRILFVGSLTYLKGLDRLRTTVEMARETGKNWEFIIATPSSLNTE
                     HFSSKPNVTIHKVVSFAELPNLYRSCDLLYFPLIYEGFEMVTLEALSSGLPVVGTDVG
                     AISVFCEAEFPGVYRIEPDNFAQVSDTFKIAAAQWKNPAEKHRLHNLIDENYGISIWS
                     QKLIEIVEACYE"
     assembly_gap    1526..1625
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1626..2232)
                     /locus_tag="PLQ30_06825"
     CDS             complement(<1626..2232)
                     /locus_tag="PLQ30_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02195.1"
                     /translation="MGKTSNFAQNIGESKSGHILRSRKIIIISPNGLSDAGGVERVML
                     YAFRALSEHGFQVGIIEQKALAKSKIGQFFSIFKRGVLGFLGQSFGLSIIANRERRSG
                     AFIINNGYAAFFARSDILFCHGCVRAARLTQHRVCLEQHRLDLSKLQEHFDNTEIPRP
                     IRLIESLKLSIYGPDEIAEMIAGNRARIIVAVSEHSQRDWQR"
     gene            complement(2235..3695)
                     /locus_tag="PLQ30_06830"
     CDS             complement(2235..3695)
                     /locus_tag="PLQ30_06830"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HPW02196.1"
                     /translation="MNTELHESEPSIVSNFLFSTLSTFLTILVPLITYPYVARVLGPE
                     NLGRTGIASSFTNYFIAAAQIGLPVYGVRLVAQRRSDRKALETGVSELVELSIVSSFL
                     AILAFSIAVMLIPKYRSEWRLFFVFGLTIITSAMNLDWLFQGMERFRYIGIRNAILQV
                     LFVVSLFLFIRKTSDYIVYAFLFSAVGFATMAINYVAAHKNIKVRFRGLSPLKHLSSL
                     LVITLFSFLVTIYTNLDFLFLSLFSKTEEAGFYSISIRIARIVIAFTATLSTVLMPRL
                     STLVNAAKEESTRLLQNSFSAIALFVIPAVFGLVAIADDLIQIFAGARFAPAASSMRI
                     TAWIVPIVAFSNALQMQILIPQQKEKSMLISFSIGIVVTGLVLCFAVKPFGQVGAALG
                     MLAGETSVLVAHICFCGRKAISMVFSRKRLFHYLLGGALCGLVAYFISKLSGIMIVVR
                     LILAIGTSGLFYLAFLLAVKDDFALFILRSGNKNTR"
     gene            complement(3692..4516)
                     /locus_tag="PLQ30_06835"
     CDS             complement(3692..4516)
                     /locus_tag="PLQ30_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02197.1"
                     /translation="MVHIKLSNAKISLFLILSLVGAKVVYADSLPSLRAVLPKLTSDE
                     IQRLLANNLIIEDYMENSSPRLLPSGQTAMTLQESLPDRKEGSKYQTAYLIDAVRFSD
                     TDKLIFLNRLAQLDTLSGITYYSETRGKETILFDDVYVVETPGSRIPKPVRPFSKIPE
                     ELSLFVHIKDANFGSTWFSVSLFEKDNIVFFLIENVQPMGIGPIRAFDAKDFRIAFSF
                     KEVQEGLLVSSVSYFNPRKLASQFVDIFSAAEKRIRAISNWMAAQAIEAKNNSGHR"
     gene            complement(4503..5945)
                     /gene="wbaP"
                     /locus_tag="PLQ30_06840"
     CDS             complement(4503..5945)
                     /gene="wbaP"
                     /locus_tag="PLQ30_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate galactose
                     phosphotransferase WbaP"
                     /protein_id="HPW02198.1"
                     /translation="MNILDLLHTHCPSNQKKKNLLMIGGLLIGDLFAVMTSFGSGFFL
                     ENLIDMQGINFKSFVTYWPYLPAFIVMLSLLNLYPGITLSAPEKLRRFTIASFLAHAG
                     IILSLYISAKRFDQYQTAFLFSWVFSIPLFFYCRTVSESLLVRHKMWGIPAVIFGAGE
                     TGKLVVDRLLDNLKLGYIPVLLLDDNPLLAGEYRGVPIITGINLGSRIASSGEVQTAI
                     IAMPEVDHRRLGEIIVQQAMEFRRYVIIPDIIGISSLWMTVQDLDGILGLLTDQELLE
                     PRNMAIKRLMDIVLSIIGMILLLPIFIIIAILIKLDSPGPVFYRHHRLGKDSKPIEIL
                     KFRSMYNNSSQMLERLLQDSPEARTEWQSNFKLKNDPRITRIGAFLRRTSLDELPQLW
                     NVLKGDLSLIGPRPIINNEVIKYGDNYWLFSKVKPGLSGLWQISGRSETDYEQRIALD
                     VYYIQSWSLWLDLYILLKTIIVVLKGDGAY"
     gene            6035..>6872
                     /locus_tag="PLQ30_06845"
     CDS             6035..>6872
                     /locus_tag="PLQ30_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW02199.1"
                     /translation="MDKLAKQQKISQRFGLGYFMLFAMYGISSPYLQLMVRKLGYSPA
                     AVGLFVGFFELVGITGPVFIAQIADRFGKLKPFLYGSAIMTIAGLVLLVPFRVPLITL
                     LSLTLLSIGLKTPVPVLDTSLLRAIESSNKKGEKAPNYGIIRAIGSAGFVAVALIAQS
                     IPGFDSSSTATMAIALGILAILFLLSIPSLPEIGVPNPRKDKIRFSFKWMDATYLIGL
                     GVIALGRLAMAPIGSFFSMYLTEELHWHAVGAMWALSATCEIPFIVLSWKLIQGKSPM
                     VAI"
BASE COUNT         1990 a         1465 c         1435 g         1882 t
ORIGIN      
        1 gccaaaggcc ttttgtaagg cctctttatt tttagtaaga aaataccagt tatttccgag
       61 tgcagattta aagtagctca attttctaat caaaaaattg ctagaaccat cgtgcgctcc
      121 accaatagca atcgagcgtg gaagattggc ctggatagct cggggctcgt atatgatttt
      181 tgcgcctgtg ttgtgcgctc ggattaaaaa atcggtctct tcacgatagc aattgccaaa
      241 gtagtgctca tcgaagcaaa tcttttccgc caactcgcga gagacgagaa aggaggcttg
      301 cacaaagggc acttcaactg gctccttagt tcttatatta aaacgagctt cgaggcttac
      361 aggatcgatg agccttttat taattttggt tttttgcaat cgttctgatt gctcaatctc
      421 tccaagaatc tcctcaaaga tagaagaaaa gtctttatcc ttggatagcc ttgttatata
      481 gggcgcacaa gcgcctacaa tcaaagcacc atagtcgtgc aaagttgtta taagtcgttc
      541 caaagagcca ggcaaaagaa ctgaatcgtc atccccaaaa tatagatatt ccccgcgcgc
      601 atttgagata cctaggtttt ttgcatgggg ctgcttgcga tttgcatctg ttctgatata
      661 gcggattcgc ggatcgtctg ctgcaagact ttcgaccacc tcgcgagttg tatctggaga
      721 acaatcgtcc accacaataa gctcgctggt ctctggctgt aggtatgtgg gaagggtaag
      781 agaaagcaag tgagctcgct tatatgtagg cacaatgaca gaaatagatt gttcactcat
      841 agcatgcttc cacaatctct atcagcttct gtgaccatat cgaaattcca taattctcat
      901 cgataagatt atgcaatcga tgtttctcgg cagggttctt ccattgagcc gcagctattt
      961 tgaaagtatc ggagacttga gcaaaattgt cgggttcgat gcgatacact ccaggaaact
     1021 ctgcttcaca aaaaacagaa attgccccta catctgtgcc cacaaccggc aatcctgaag
     1081 agagcgcttc cagcgtgacc atttcgaatc cttcatatat caaagggaaa tacaagagat
     1141 cacaggaacg atagagattg ggtagttcag caaagctaac taccttgtgg atagtaacat
     1201 ttggctttga agaaaaatgt tctgtattta aagatgaagg tgtcgcgatt atgaactccc
     1261 aattcttccc tgtttctcga gccatttcaa ccgttgttct tagcctatcg aggcctttga
     1321 ggtatgtaag tgaccccaca aagagaattc gtagcactct tttagattgt ttcgaagatt
     1381 cttgttcgtt taattccgaa gtaggctgta aatgttttgt atctacggta ttgggaaggt
     1441 gctcaaattt gccagatgaa agatgataat atcgctgcca atccctttgt gaatgttcgg
     1501 ataccgctac gatgatacgt gcacgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnatcgc tgccaatccc tttgtgaatg ttcggatacc gctacgatga tacgtgcacg
     1681 attccctgcg atcatttctg ctatttcatc tggtccatat atcgagagct tcaagctctc
     1741 gataagccta ataggcctgg ggatttcagt attatcgaaa tgctcttgga gctttgataa
     1801 atccaacctg tgctgttcta aacacactct gtgctgtgtt aagcgtgcag cccgaacaca
     1861 gccatggcaa aacagaatat cggatctggc aaaaaaagca gcatatccat tattgattat
     1921 gaaagcacca gatctccgct ctctattggc aattatcgat aatccaaagg attgtcctaa
     1981 aaaacctaac actcctctct tgaaaataga gaaaaattga ccgattttgc tctttgccaa
     2041 ggctttttgt tcaattatac caacttgaaa accatgttcc gaaagagcac gaaatgcata
     2101 gagcatgact cgctctacac ctcctgcatc agagagtccg ttgggagaga ttattattat
     2161 ctttcgagac ctaagtatat ggccggattt ggattcccca atattttgcg caaagttcga
     2221 ggtctttccc atatctatct cgtattctta tttccgcttc ttaagatgaa gagcgcaaaa
     2281 tcgtccttga ctgcaagaag aaatgcaaga tagaaaagtc cacttgtacc aatagccaat
     2341 ataagcctta caacaatcat tatccctgat aatttgctta taaaataggc aactagccca
     2401 cagagtgctc ctcctaaaag ataatggaat agtctctttc gtgaaaacac catagaaatc
     2461 gcctttctcc cgcagaagca tatatgtgcg acaaggactg atgtctctcc tgccaacatg
     2521 cctagggcag cccctacctg gccaaaaggc ttcactgcaa agcatagcac aaggccagtc
     2581 actacgatgc cgatgctgaa agaaattagc atagacttct ctttctgttg tggtatcaag
     2641 atttgcatct gaagcgcatt cgagaatgcc actatcggta cgatccatgc cgttattcgc
     2701 attgaagatg ctgcaggtgc aaatctagcg ccagcaaata tttgtattaa atcatcggca
     2761 atagctacaa gcccaaaaac tgcaggaatt acaaataatg caatggccga gaaagaattc
     2821 tgcaaaagcc tcgtactctc ttctttagcc gcattgacaa gtgtcgaaag gcgaggcatg
     2881 agcacagtac tgagcgttgc tgtaaaggca atgacaatcc tagcaatcct aatcgagatg
     2941 ctatagaatc ctgcctcctc tgtctttgaa aacaagctca aaaataaaaa atcgaggttt
     3001 gtatatattg tcactagaaa agaaaaaagc gtaattacta ggagtgaact aagatgctta
     3061 agaggcgaaa ggcctcgaaa cctaaccttg atattcttat gcgcagctac ataatttatg
     3121 gccattgtcg caaatccaac agcggagaat aaaaatgcgt aaacaatata gtccgaagtc
     3181 ttgcgaataa agagaaagag cgataccacg aatagcacct ggagaattgc attgcggatg
     3241 ccgatatatc gaaagcgctc catgccctga aaaagccagt ctaaattcat ggcggaagtt
     3301 atgatagtca atccgaatac aaaaaaaagt ctccactcgc ttcgatattt cggaatgagc
     3361 atcacagcga tagaaaaggc caagatagct aaaaatgagg agactatcga aagctcaacg
     3421 agttctgaaa cacctgtctc aagcgccttt ctgtcggatc tacgctgggc tacgagcctg
     3481 acaccgtaga ctggaagccc aatttgcgct gctgcaataa aataatttgt gaatgaggaa
     3541 gcaatacctg tgcgaccgag attctctggg ccaagaactc ttgctacata gggataggta
     3601 ataaggggaa caagtatggt caagaaagtg ctaagggtcg aaaaaagaaa attgcttact
     3661 atcgatggct cagactcatg taattcagta ttcatcgatg ccccgaatta tttttggctt
     3721 ctattgcttg tgctgccatc cagttactga tagctcgaat tctcttttct gctgcagaga
     3781 aaatatccac aaattgagaa gccaattttc ttggattgaa atagctcaca ctgctgacta
     3841 gaagaccttc ttgtacctcc ttgaaagaaa aagcgattcg gaaatctttt gcatcaaaag
     3901 cgcggatagg ccctatgccc atgggttgca cgttttcgat taagaaaaag acaatattat
     3961 ccttctcaaa aagcgatacg gagaaccatg tagatccaaa atttgcatct tttatgtgta
     4021 caaagagact cagttcctcg ggaatcttgg aaaaaggcct taccggctta ggaattcgcg
     4081 atcctggagt ttctaccaca tagacatcat cgaataatat cgtctccttg cccctagtct
     4141 cggaataata agtaattcct gataaagtat ccagttgtgc tagcctatta agaaatatca
     4201 gcttatctgt atcagaaaac cgaacagcat cgattagata tgcggtctga tatttgcttc
     4261 cctcttttcg atctggtaga ctctcttgca gggtcatagc tgtttgaccc gaaggcaaaa
     4321 gtctgggcga actattctcc atgtaatcct caataataag attattagcc aaaagcctct
     4381 gtatctcatc gcttgtcagc tttggcaaga cagcccttag cgatggtagg ctatctgcat
     4441 acactacctt tgctccaaca agagaaagaa ttaagaatag agatatcttt gcgttcgata
     4501 atttaatatg caccatcacc tttgagcact acaataattg tcttgaggag tatatagaga
     4561 tcgagccaaa gcgaccagct ttgaatatag tatacatcaa gcgcgatcct ttgctcatag
     4621 tcggtctcag aacgaccaga tatctgccat aagcccgaca gcccaggctt tactttggaa
     4681 aatagccaat agttatctcc atattttatt acttcattat taattatagg tcgaggacca
     4741 atcagactga gatcgccttt tagaacattc cataattgtg gcaattcatc taaacttgtc
     4801 cttcgtaaaa aagcgccaat cctcgttatt cgaggatcgt tttttagctt gaaattagat
     4861 tgccattctg tccgtgcctc tggactatcc tgtagcaatc tttcaagcat ttgagaagaa
     4921 ttgttgtaca tcgaacgaaa tttaagtatt tcgataggtt tgctatcctt acctagtcga
     4981 tgatggcgat agaatactgg cccaggagaa tcgagcttta taagaatcgc gataatgata
     5041 aaaatcggca aaagcaatat cataccaata atgcttagta ctatatccat caagcgctta
     5101 atcgccatat ttcggggctc gagaagctcc tgatcggtaa gcaagcctag aattccatca
     5161 agatcctgca ccgtcatcca caagcttgaa atacctatga tatcaggaat aataacataa
     5221 cggcggaatt ccatcgcctg ctgaacgatg atttcaccta aacgcctatg atccacttct
     5281 ggcattgcta taattgcggt ctggacttct cccgaagatg caatacgaga accaaggtta
     5341 atacctgtaa taatgggaac gcctcgatat tcacccgcaa gaagaggatt gtcatcgagc
     5401 agaagcacag ggatataacc caatttgaga ttatcgagaa ggcgatcgac aacgagctta
     5461 cctgtctccc ctgctccaaa tatgacagca ggaattcccc acatcttatg ccttacaagc
     5521 aatgattctg aaacagttct gcagtaaaag aaaagaggaa tactgaagac ccaggagaac
     5581 aagaacgccg tctgatattg atcaaatctt ttagcgctaa tatataggga aaggataatt
     5641 cctgcatggg caaggaacga ggcaatagta aaacgtctta acttttctgg tgcggaaagg
     5701 gtaatcccag gatagagatt tagaagcgat aacatgacta taaatgctgg aagataaggc
     5761 caataagtaa caaatgactt gaaattgatc ccttgcatat cgataaggtt ttccaagaaa
     5821 aaacccgagc caaaacttgt catgacggca aaaagatcac ctatgagaag tccacctatc
     5881 atgagtagat tttttttctt ttgattggaa ggacagtgag tatgcaaaag atctaagata
     5941 ttcatttcca ttaataatca ttttacatat tctttgctat tcttgcaaca aatgttgacg
     6001 catctgcccc ttaaccctat tctttcaagt agttatggac aagcttgcaa aacaacagaa
     6061 aatatcgcag cgttttggac tcggttactt tatgctcttc gcaatgtatg gtatttcctc
     6121 accgtatctc cagctcatgg tgcgaaaact tggatatagt ccagcggcgg tcggcctttt
     6181 tgttggtttt ttcgagcttg tgggtattac ggggccggtg ttcattgctc agattgcaga
     6241 caggttcggg aaactaaagc cttttctcta tggctcggcg ataatgacta ttgctggcct
     6301 tgtgctgctt gtgccttttc gggttcctct gattacgcta ttgagtctta cgctgctgtc
     6361 aattgggcta aagacgcctg tacctgtact ggacacttct cttttgcgcg ccatagagtc
     6421 ctccaataaa aagggagaaa aggcgccgaa ctatggcatt attagggcaa ttggctcggc
     6481 tggtttcgtg gcggtggcgc ttatcgctca gtcgatccct ggattcgatt cgagttcgac
     6541 ggcgacgatg gcgattgcgc tggggatact ggcgattcta ttcttgttga gtatcccttc
     6601 tttgccggag atcggcgtgc caaatccgcg caaggacaag atcaggtttt ctttcaaatg
     6661 gatggatgct acgtatctga tagggcttgg tgttattgcg ctgggacggc tggccatggc
     6721 tcctatcggg tcattttttt cgatgtatct cacggaggaa ttgcattggc atgcggtggg
     6781 agctatgtgg gctttgtcag caacctgtga aattcccttc attgtgctgt catggaaact
     6841 tattcaggga aaaagcccga tggtggcaat tg
//
LOCUS       DAOMZL010000085         6851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_10386_length_6851_cov_14.629194, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000085 DAOMZL010000000
VERSION     DAOMZL010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10386_length_6851_cov_14.629194"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PLQ30_06850"
     CDS             complement(<1..623)
                     /locus_tag="PLQ30_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02200.1"
                     /translation="MLNKKRDREFKFIIDRKARENSRKDIPVQSKKRQYLLAAILIIL
                     TLSSCDNTFSIFQSIGQEVKQLGAELFKGTTVRAMGSDATNYYALMSKVVYKVYKPKS
                     GVNWDVLPIDGDTDYFSPGLASDGNTIYVAKADLNANLKDIYSSTDGCKNWTGMDAKN
                     DIQNVIGAGNNFCVDWLKCENNTLFVAFHSSAGYSLFYYDVASTSFK"
     gene            complement(634..1230)
                     /locus_tag="PLQ30_06855"
     CDS             complement(634..1230)
                     /locus_tag="PLQ30_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02201.1"
                     /translation="MLKKQMLVTLMISALALGLSAQEKIEDPFEATRVKGDQSISIQI
                     GGFLPLFVIDNAGTPISPSNMSIGSAFSVQYRYMLGKNFSIGGSGAGSFVTTVGGGTL
                     FMAPIGITGAWIWGREPMEYEVSAELGMNIMRLYGNGLISPIAKFGGGISRFVDSSWS
                     IGFKLNWWFVPEIHLGTFSYLTSYANFLEFSIGANYHF"
     gene            complement(1240..1791)
                     /locus_tag="PLQ30_06860"
     CDS             complement(1240..1791)
                     /locus_tag="PLQ30_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02202.1"
                     /translation="MLKRKSFDWTPRNSHDVLMALPEGIVCGLFEHRNGQRIDHKNRD
                     FLEHVQRTLVRHSQEICKSFPDIFALSAVEKNGDHTILKLDKAIDSQNLDIALELFAE
                     DADLKVSDNALFYGNKMREGIWFGNGCAVSLDAAISFKKYELMLYLAELPEASFCGFH
                     FRAFARVYCREKMHSHSKEDQII"
     gene            1967..2737
                     /locus_tag="PLQ30_06865"
     CDS             1967..2737
                     /locus_tag="PLQ30_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02203.1"
                     /translation="MKRKSEEISCFLIDTISRWPGVDCISMDKRSQQDELDPHYALVL
                     DVYYRRAIPTMEKRQMLFDNPGAFESARGGMKDRFFLDEIPIRVEYKHIPSIQNLVEH
                     PLNHIKLLKNSGTYPLYRLLHFSLVFSRSEWIERMRSNLTNFPEEAWNTLFDSFAAKM
                     EHYLSDFGAASVSGNQFFRLMSHSGFLRYAGASIFMVNHVFEPSHAEFESKLKAQPRL
                     PANFMNLWDSIAGDKNGISEFKKFELARLLAREIFELR"
     gene            2770..4533
                     /locus_tag="PLQ30_06870"
     CDS             2770..4533
                     /locus_tag="PLQ30_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02204.1"
                     /translation="MSKIQIAHKPVRWFAHENKGLFRNILTLCVFLCFLFLLLFSSCR
                     SREVHAFGWYDKDGQSLAFTSLLIDKNVGIFERQGEKEKYNLIKAIEIPEGYGIRLLV
                     SSLSNETEFLLNFGEERGKSKSIHCSISEAGSYAFVIPLSEIESIKWLEIKVLKLGSN
                     MSEGNINSLKTASKDDSNAAIDGSSDLSLFSVKELRFVPPFRGMRIQNDITEISPHFL
                     YEEQGEKKHYEIMAPFSGLSPELSSRMKVEIILEGSPGLATFFWGNQKISYLHQGKRS
                     LFVIPYTIFENSPGKIAMDVENDIRASVFCVDPLQSAEEPPSIDPGLLVFHSPLIADP
                     YYLARWDQQPEVLLFLFKDYSVQDRYLKRLAFFVEKMGFVGSLAQDSQIASLHGWNAH
                     DYRAEDLARFFDAAATQNFSLSNEEIELRELLIKNGIILKSGGKYRPGIGAVVSISQE
                     SPSYLQYRLLTHELSHALFFTDTSYRDLVISLYQRLTNDERWFLDRYFRWMRYNVNSS
                     YLMANEMQAYLVQQPIQDLEKYFSRTLANRLVEEHPELTEDINFYMEEHLDALVSCAE
                     QLGSFLESTYGFEPGMLFRAR"
     gene            complement(4654..5259)
                     /locus_tag="PLQ30_06875"
     CDS             complement(4654..5259)
                     /locus_tag="PLQ30_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02205.1"
                     /translation="MGKNTKIPPPSKKHEDLLEGLFISPLDDSESLKKMETEEMKILS
                     PLEGRAPLEESHSIPFDDSQGKRVFPLDNTNKREAPEISTRENPSGPESSAQEQLLLR
                     FANELKSIKQDLLSIKEHFKVIREPQIQPSVSPTSVESPSDRLSGASEVQQQALIDEV
                     KRLLLYLDRLLESLPEEKIEEFANSEYFNLYRHVFDELNLS"
     gene            5347..5895
                     /locus_tag="PLQ30_06880"
     CDS             5347..5895
                     /locus_tag="PLQ30_06880"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPW02206.1"
                     /translation="MPKIWLMYSPHSSFKTSKRLLGSLSYGLFSFLIAYCFISVVAGK
                     AGILAYKDLALQKQRIQIAIYSLQTQNENKMNTIEELKSNPYAAAEMAAALGYIHQGE
                     VLITLPESWRDAGQIKNNEVNTPILAGESTGLPDPLIRVMSAITGILVFLAIQLFQYK
                     SIEQSAKRISLENRTEPIRTQI"
     gene            complement(5928..>6851)
                     /locus_tag="PLQ30_06885"
     CDS             complement(5928..>6851)
                     /locus_tag="PLQ30_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPW02207.1"
                     /translation="SVLAWQALAHSMNVASVKVLQTVGFDAAISRAAALLDITDPIEI
                     RKTFPRYYPLALGVIGVTPLKMARAYAVFANGGKAITPIAIRYIEDRYGNIIAEPEKD
                     ALQTLRQKSPQVISSQNAAIMIDMLKRVVFSGTLAGTTQSGSIFRQKTADGHSYVIPI
                     AGKTGTTENWADAWTIGSSPYYTTAVWLGFDRPGNSLGVSQTGATLAAPVWANYMRDI
                     HLGLQYKNFPKPDSGLISATVCSLSGQLPTEFCSDGTINLLFLEGTEPTHFCELHHPV
                     IAPEPIDSTDRLDFEEPILSRPEESPIFQNR"
BASE COUNT         1925 a         1442 c         1462 g         2022 t
ORIGIN      
        1 ttcttgaatg aagttgatgc tacatcataa tagaaaagag aatagcctgc tgagctatgg
       61 aaggccacaa aaagcgtatt gttctcgcat ttgagccaat caacgcaaaa attattgcca
      121 gcaccaatta cattttggat gtcgttcttt gcgtccatac cagtccagtt cttacatcca
      181 tcggtactcg aatatatatc tttcaaatta gcgtttaaat ctgctttggc aacatatata
      241 gtattgccat cggatgcaag acctggtgaa aagtaatctg tatctccatc tataggaagg
      301 acatcccaat ttacgccgct ttttggcttg tataccttgt ataccacctt agacatgagg
      361 gcataataat ttgtagcatc cgagcccatt gcgcgcacgg ttgttccctt aaaaagctca
      421 gcgcccaatt gcttcacttc ttggcctatg ctctgaaaaa tcgaaaaagt attgtcacag
      481 cttgacaagg tcagaatgat cagaattgca gctaaaagat attgcctctt tttggactgg
      541 actgggatat cctttctgga attctctcga gcttttctat caataataaa cttgaattct
      601 ctgtctctct tcttattaag catgtggcac ccctcagaaa tggtaatttg caccaatgga
      661 aaactccaga aaattagcat aactggtcaa atagctaaat gtccccaggt gtatctctgg
      721 aacgaaccac caatttaact tgaatccaat actccatgag gaatctacga atctggagat
      781 tcctcctccg aacttggcaa taggagaaat aagaccatta ccatagagtc gcataatatt
      841 catacctagt tcggcagaaa cctcatattc cattggttcg cgaccccata tccatgctcc
      901 ggtaattcca atcggagcca taaaaagcgt gcctccgccc acggtagtca caaaggagcc
      961 tgcacccgaa ccgcctatgg agaaattctt tcccagcata tagcgatatt gaactgaaaa
     1021 tgccgatcca atactcatgt ttgatggtga tataggagtt ccagcattgt cgatcacaaa
     1081 aaggggcaaa aagcctccga tttgtatgct tattgattgg tcccctttga ctcgtgtcgc
     1141 ctcaaagggg tcttcgattt tttcttgcgc actaagacca agcgccaagg cggaaatcat
     1201 tagagtaacc aacatttgtt ttttcagcat cagccctccc taaattatct gatcctcttt
     1261 tgaatgagaa tgcatcttct ctctacagta gacacgagca aaagctctga agtgaaaacc
     1321 acagaacgat gcttctggta actccgctag atatagcata agttcatatt tcttgaaact
     1381 gatggctgca tcgagagaaa ctgcacatcc attgccaaac cagatccctt cacgcatctt
     1441 attaccatag aaaagagcat tgtcggatac tttcagatca gcatcttcag caaaaagctc
     1501 aagagcaatg tcaagatttt gcgaatcgat tgctttgtca agcttcaata tcgtatgatc
     1561 accattcttt tccactgccg atagcgcaaa gatgtcaggg aagctcttgc agatttcttg
     1621 agagtggcgc acaagggttc gctgaacatg ctccaagaaa tcgcgattct tatgatctat
     1681 tctttggcca tttcggtgtt cgaataagcc acatacgatg ccttcaggca atgccatcag
     1741 aacatcgtgg ctatttcttg gagtccaatc aaagctcttt ctcttgagca tccatatctg
     1801 ttttctaata tttccataag gccttacaat tagtacaaag atctgcatgt caattgagta
     1861 tatagtcact taggctcttg gttaacatgg gtgaaagagt accatcgaag atgcatggca
     1921 gaaagccagc gtccatgata taattggcct aggaagggta gtgtacatga agcgcaaatc
     1981 tgaagagata tcgtgctttc ttatcgatac gatttctcgc tggccaggtg tggattgcat
     2041 atctatggat aagcgctccc aacaagatga attagaccca cactatgcat tggttctcga
     2101 tgtatactat cggcgcgcca ttcctaccat ggaaaaaaga caaatgctct tcgacaatcc
     2161 aggtgcgttc gagtcggctc gtggtggtat gaaggatagg ttttttctag atgaaatacc
     2221 cataagggta gagtataagc atataccaag cattcagaat ctggtagaac atccgcttaa
     2281 tcatataaag ctcctaaaga attcaggtac ttatcctctc tatcgcttac ttcatttttc
     2341 gcttgttttt tcgaggagcg aatggatcga acgcatgcgg tctaatctca ccaatttccc
     2401 ggaagaagct tggaatacgc tattcgatag ctttgctgcg aagatggaac actatctctc
     2461 cgactttggg gcagcatcgg tatcaggtaa tcaatttttt cgcttaatgt cgcactcagg
     2521 atttctaaga tatgcaggtg cttcgatttt tatggtgaat catgtttttg aaccatctca
     2581 cgccgaattc gaatcaaagt taaaggctca gccaagactc ccggcgaatt ttatgaacct
     2641 atgggattca attgccggcg ataagaatgg tatttcggag ttcaagaaat tcgagctcgc
     2701 tcgattgctt gcacgagaaa tcttcgagct ccgatagaca aaagccttag atagagatct
     2761 tagatagaga tgagtaaaat acaaatagcg cataagccag tccgatggtt tgcgcatgag
     2821 aataagggtc tatttcgcaa catactcact ctctgtgtat ttttgtgttt cttatttctc
     2881 cttcttttta gctcctgcag atctagagaa gtccatgctt ttggatggta tgataaggat
     2941 gggcaaagtc tagcatttac atccctcctg atcgataaga atgttggtat cttcgagaga
     3001 cagggcgaga aagaaaaata caatctgatt aaagcaatag aaattcctga aggatatggt
     3061 attcgtctac tggtatcatc tttgagcaat gagacagaat tcttgctcaa ttttggcgag
     3121 gaaagaggta aatcaaagtc aatacactgc tcgatttcgg aggctggaag ctatgctttt
     3181 gtcattcccc tttcagagat cgaatccatc aaatggctcg agataaaagt gctaaaactt
     3241 ggttccaaca tgagtgaagg caatattaat agccttaaga cagcttccaa agatgattcg
     3301 aacgcagcaa ttgatgggag ctccgatttg tctcttttct ctgttaagga gctacgtttt
     3361 gtacctccgt tccgcggtat gcggatccag aatgacatta cagaaatatc gccccatttt
     3421 ttatacgaag aacaaggcga aaaaaaacac tacgaaatca tggctccttt ttctgggctt
     3481 tctcctgaac tttcttcaag aatgaaagtg gaaatcatat tggaaggaag tccaggtctg
     3541 gctacctttt tttggggaaa tcaaaagata agctatcttc atcaaggaaa gagaagcctc
     3601 tttgtaattc cttatacgat ttttgagaac agccccggta aaatagccat ggatgttgag
     3661 aatgacatca gagcctcggt tttttgtgtt gatccattgc agtcggccga ggaaccaccc
     3721 agtattgacc cgggtttgct cgtttttcat tctccgctga ttgcagatcc atattacctt
     3781 gcgcgatggg atcaacagcc cgaagttctt ttgttcttat tcaaggatta ttctgttcaa
     3841 gaccgttatc taaagaggct tgcttttttt gttgaaaaga tgggatttgt tggaagcctg
     3901 gcgcaagaca gccagatcgc gagcctccat ggctggaatg ctcatgatta tcgagcagag
     3961 gacctggcga ggttttttga tgcagccgct acccagaatt tctcgctatc gaatgaggaa
     4021 attgaattga gagagctatt gataaaaaat ggaattatct taaaaagcgg aggcaagtat
     4081 cgaccaggca taggggcagt agtatcgatt tcgcaagaat ctcccagcta cctgcaatat
     4141 cgcctgctca ctcatgagtt gagccatgcc ctttttttta cagataccag ctaccgcgac
     4201 cttgtcattt ctctctatca gagattgact aatgatgaaa gatggtttct cgatcgttat
     4261 ttccgctgga tgcgatataa cgttaattca tcatatttga tggctaacga aatgcaggct
     4321 tatttagttc agcagccaat tcaagattta gagaaatatt tttcaagaac attagcgaat
     4381 agacttgtag aggagcatcc tgagctaaca gaagatatca atttttatat ggaagagcat
     4441 cttgatgcac ttgtgtcatg tgctgaacaa ctgggttctt ttctagagtc tacttatgga
     4501 tttgagccag gcatgctttt tagagcaagg tagagcaagg tagccgcact gggatttttt
     4561 gcgctattga atatcggcct tcatttctgg tttttccttt atgcatgcta tgattctgtt
     4621 gtggatttct cgaattatag tttccaagat tgcttaagaa aggttcagct catcaaatac
     4681 gtgtctatat agattaaaat attcagaatt tgcaaattct tcgatttttt cttctggcaa
     4741 tgattccagg agacgatcga ggtagagcag cagtctcttc acttcatcaa tcagggcttg
     4801 ctgttggact tccgaagctc cagataatct atcggaagga gattccacgg atgtgggaga
     4861 taccgaaggt tggatctgcg gttcccttat aaccttaaaa tgttctttta tagatagaag
     4921 atcttgcttg attgatttca attcattggc aaatctcagt aaaagctgtt cctgagccga
     4981 gctctctggt ccggatggat tttctcttgt gctgatttca ggcgcttccc ttttgttcgt
     5041 attgtccaaa gggaagaccc gctttccttg agagtcatcg aaaggaattg agtgagattc
     5101 ttctaaagga gctcttcctt ctaacggaga gagaattttc atttcttcgg tttccatttt
     5161 tttgagagac tccgaatcat caagagggct tataaaaagg ccttcgagca agtcttcatg
     5221 ctttttgctc ggtggtggta tcttggtatt tttccccatc gcggtcttcc tctttaagaa
     5281 tactacaccc tttaactcat acagggaact acttcctgca gaaatgcctc atcagaattg
     5341 atatttatgc cgaaaatttg gttgatgtat tcgcctcatt cttcattcaa aacaagcaag
     5401 aggctgcttg gctctctttc ttatggctta ttctcatttc tgattgcgta ctgctttatc
     5461 tctgtcgttg caggcaaagc aggaatacta gcctataaag atctagcctt gcaaaaacaa
     5521 cggattcaaa tagcaatcta ttctcttcag actcaaaatg agaacaagat gaatacaatc
     5581 gaggaactta aaagcaatcc ctatgctgcc gcagagatgg ctgcggcttt aggatatatc
     5641 catcaaggag aagtactgat cacacttccg gaatcatgga gagatgccgg tcagataaag
     5701 aacaatgaag taaacactcc cattttggca ggagagtcta caggactacc ggatccactg
     5761 attcgcgtta tgagtgcaat cacaggaata ctcgtttttt tggccataca gcttttccag
     5821 tacaaatcca tagaacagtc agcaaaaaga ataagcctag agaatcgaac cgagccgatc
     5881 cgcacccaga tttaataagt ttgtcaactt aagtcctact catattctta tcgattttga
     5941 aaaataggtg attcttcagg tcttgaaaga ataggttctt caaaatctag ccgatctgtc
     6001 gagtcaatag gctcaggagc aatgacaggg tgatgcagtt cacagaaatg agtgggctct
     6061 gtaccttcca gaaaaaggag atttattgtt ccatcagaac agaattcagt tgggagctgt
     6121 ccggagaggg agcaaaccgt tgcagaaatc aggcctgaat caggcttggg gaagttctta
     6181 tattgcaggc caagatgtat atcgcgcata taattagccc aaactggagc tgcaagtgtg
     6241 gctcctgttt gagaaacccc aagagagttg cctggacgat cgaagccaag ccatacggcg
     6301 gttgtgtaat atgggcttga accgatagtc catgcatcag cccaattctc cgttgtgcca
     6361 gtcttacccg caatgggaat tacatatgaa tgtccatccg ctgttttttg cctgaagata
     6421 gagcctgatt gtgttgtgcc ggcaagagtt cccgaaaata caactctctt tagcatatcg
     6481 atcattatcg ctgcattctg cgatgatatt acttgcgggg atttttgccg gagggtctgt
     6541 agtgcatctt tttcaggttc agcaatgata ttgccataac gatcttcgat atatcttatg
     6601 gcaatgggtg ttattgcttt tcctccattt gcaaatactg cataggctct tgccattttg
     6661 aggggtgtga caccgattac tcctagcgct agtggataat atcgagggaa ggttttacgt
     6721 atctctattg gatccgttat gtcgagaaga gccgctgctc gcgatatagc tgcatcgaag
     6781 ccaactgttt gaagcacctt gaccgatgcg acattcatag aatgagcaag ggcttgccat
     6841 gcgagaacag a
//
LOCUS       DAOMZL010000086         6362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_11448_length_6362_cov_14.377676, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000086 DAOMZL010000000
VERSION     DAOMZL010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11448_length_6362_cov_14.377676"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2114)
                     /locus_tag="PLQ30_06890"
     CDS             complement(<1..2114)
                     /locus_tag="PLQ30_06890"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW02208.1"
                     /translation="MNRIEHHPILTAKKNLTLVKFSFDGEEITGYEGEPVSSALISAD
                     RHVFSYHFRDDFPQGLFCANGQCSQCTMLIDGIPKKSCVTPLAQGMDVRTIHGLPRLP
                     SQDRPLVQAERRSIRTDILIIGAGPAGLAAAAELGNMGFQVLVADDKQQAGGKLVLQT
                     HKFFGSEADCYAGTRGIDIAEILKEEALKYPSVVILTNAPVVGIYKDRKAGIYLNYES
                     YLLVEFSALVVAAGAREKALVFPGWDLPGVIAAGAFQTLVNRDLVKAYSKILIVGSGN
                     VGLIAAYHALQADIEVTGIFEAAGRVNGYQVHADKIRRLGVPIHLNTTITRAEGCQKV
                     QRAYVSAVDERFQPIPGTARGYEVDAVLIAVGLAPCNEFLEQARRYGIMAVAAGDASE
                     IAEASSAIYGGKTAAANLALMLGLTKKGAIDPGWEITRKVLASRPGDSYERSPIVPGK
                     EWRPVFFCSEEIPCNPCATVCPLGAIELRSMQGNIMDLPYFAGDHCTGCGACVSICPG
                     LAITLVRRVTDDKALVVLPWEFNVDSGLEEDMELVDQKGEILERARIISRRKVQKRNT
                     WLLTFEVDVAHAARIAGVRRLKTQKPARLQEKAIELENSSSIMPVDKSLQEDLDTVVC
                     RCERITLREIVDFIKRHDVKDANQLKSLRVGMGACGGKTCSQLIAKAFSIAGVNPAEI
                     EPFTQRPLFMEVPMGSLVNEGL"
     gene            2274..3755
                     /gene="murD"
                     /locus_tag="PLQ30_06895"
     CDS             2274..3755
                     /gene="murD"
                     /locus_tag="PLQ30_06895"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPW02209.1"
                     /translation="MDEFRGKYITIMGLGLHGGGIASARFFAKAGAEVTVTDLRDEKT
                     LSPAIHELQGLPIRYVLGRHEIEDFAHADLVIKNPAVRRDSPYLAYAKRIETDISIFL
                     RYSKSPLVAVTGSKGKSTIASAIWHVLFQNNKKALLGGNITVSPLDFLDKTDAQVPVV
                     LELSSWQLGDLRGTGLLKPSVAVLSDILPDHLNYYDSMDAYVSDKRVIYESQDENCWT
                     VCNADQKWGHAFAEETLAQVLWYSERPLEDSVGKNALGGWLTKSKTKSNYSDDSGPSY
                     WSLDLLEGYGRFSLDGQEELLVPSQVLVPGRHQKKNILAAAVAARAFGLSPEGIMSAL
                     ASFPGVPHRLEFVGSIRGIKWYNDSTATIPDATIAAIDSFSSPIVLIAGGSDKMSDFS
                     AFAQKAKNLKAVILLAGSGTDRLIPILDVNKIAYNGPFFSMHEAVQAADKQADPGDIV
                     ILSPGCASFGMFLHEFARGDAFKREALMLFRQYAEQGSTAQKR"
     gene            complement(3736..4587)
                     /gene="amrB"
                     /locus_tag="PLQ30_06900"
     CDS             complement(3736..4587)
                     /gene="amrB"
                     /locus_tag="PLQ30_06900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPW02210.1"
                     /translation="MTTNRRIRKRYLPSGWYPEDSAEISSLVADWTKSQNNLSAYAVV
                     APHAGWYFSADLAARAVWALRDCDTVAILGGHLGNGEPILYGDEDGFDCTVRDAENDI
                     EVLNSLKAELKNVGIQEMAPDRDIDNSVEILLPLAGFRFPNARILWLRVPPDYKAREL
                     GSALFRAASSCKKQLVAIASTDLTHYGPNYGFMPRGLGEPAVQWVRNENDKGFISAAL
                     AMDADKVLKHARNNFSACSSGAVAAAISFALNAGARHSLLIGHKLSIDIHPDRSFVGY
                     AAIAFVP"
     gene            4706..6067
                     /locus_tag="PLQ30_06905"
     CDS             4706..6067
                     /locus_tag="PLQ30_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02211.1"
                     /translation="MKRTLRIILFIGTFVLLALIVGYVAWKIGEMRLLGEQSASAEYS
                     ILRNAASLITSEEDLGDQFVRDRLKSLYNASTHLLAVQVLDRNGLVLWKMPDESPYFA
                     SPATNPGSVFRAPGMSTIIYMTPLPDGMKLMALYSILSQRDISNILLVPAIILAAWIV
                     LLVVLQFALKERPIEASEIIGTERMPETPEKPQYHEEPLESVSYSEEALLTISDEASS
                     IAEESIENHASLENIENIEENIEEEAEEITNKKVEIPVFPQAPQPEPETIPMSISSSD
                     DFLPGEFVAPAPQIEEEAEEAPQPEIFPSTLSLAELERSLDIELRREKETSLLLVHCL
                     LSGELDPSALALGVTIRDYFASDSLVFELGKGCYAVVLPGMDAGSALKVAVDLDDVLT
                     TTASLYKDLSEEPPFYFGLSARYNRTISPDRLYREAFAALKEAQERSGSRILAFKPVS
                     SVA"
     gene            complement(6082..>6362)
                     /locus_tag="PLQ30_06910"
     CDS             complement(6082..>6362)
                     /locus_tag="PLQ30_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002679668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HPW02212.1"
                     /translation="IAQEVSNSDLLICEGMFEHALIESAVDKRHMTARQAALIARDAG
                     NIKKLGLIHYSPRYADRELKILLDEAREVFPDTVLTKDRMRFPIEFVD"
BASE COUNT         1713 a         1557 c         1389 g         1703 t
ORIGIN      
        1 tgcaaacctt cattcacaag agaacccata ggcacttcca taaagagtgg tctctgagta
       61 aaaggctcga tctccgctgg attcactcct gcaatgctaa atgccttggc gattaactgc
      121 gagcaagttt tccctccaca tgcgcccatt cctactctga gagatttaag ctgatttgca
      181 tctttgacat catgcctctt gatgaaatcg actatttccc gcaaggtaat tcgttcgcag
      241 cgacatacaa cagtgtccag atcttcttgt aaactcttat cgacaggcat tatgctagat
      301 gaattctcca attctattgc cttttcttgc agtcgagcgg gcttttgtgt cttgaggcgt
      361 cgaacacctg caatccttgc cgcatgcgct acatctacct cgaaggtcaa aagccaggtg
      421 tttcttttct gcacttttcg cctagatatt atgcgcgccc tttctaatat ctcacccttc
      481 tgatccacga gttccatgtc ttcctcgagt ccagaatcga cattaaactc ccatggaaga
      541 actaccagtg ccttgtcgtc cgtaactctt cgaaccagag taatggcaag accagggcag
      601 atcgacacac aggctccaca tcctgtacag tgatctcccg caaagtaagg aagatccata
      661 atgttgcctt gcatcgacct gagctctatg gctcccaatg ggcaaaccgt tgcacatgga
      721 ttgcatggaa tctcctctga acaaaagaat actggccgcc attctttacc aggaactatt
      781 ggagatcttt cataactgtc tccaggccgt gaagcgagca cttttctggt tatttcccac
      841 cctggatcta ttgccccttt ttttgttagt ccaagcatga gcgcaagatt tgctgctgca
      901 gttttgccac catagatcgc actcgaagct tctgcaatct ctgaagcatc tcccgcagcc
      961 acagccataa tgccatagcg tcttgcttgt tccagaaatt cattgcaggg cgcaaggccc
     1021 accgcaatga gaacagcatc tacttcatag cctcttgctg ttcccgggat aggctgaaat
     1081 ctctcatcga ctgcggatac ataagctctc tggacctttt gacagccctc tgctcttgtt
     1141 atcgtggtat tcagatgaat tggcactccc aaacgtctta tcttgtctgc atgtacctga
     1201 tacccattga ccctaccagc tgcttcaaaa ataccagtga cttcgatgtc tgcttggagg
     1261 gcatgatagg cagcgattaa gccaacatta cctgaaccta caatgaggat cttgctatat
     1321 gctttgacaa gatcacgatt tacaagcgtc tggaaagctc ctgccgctat tactccagga
     1381 agatcccagc cgggaaaaac cagtgctttc tctcttgctc cggcagcaac gacgagagca
     1441 ctgaattcga caagcagata ggattcgtaa ttgagataaa tcccagcttt tcgatctttg
     1501 tagataccca ccactggcgc attcgtcagt atgactaccg aagggtattt cagtgcttcc
     1561 tctttcagga tctcagcaat atcgatgccg cgtgttcctg catagcaatc ggcttctgat
     1621 ccaaagaatt tatgagtctg caaaactagt ttgcctccgg cttgctgctt gtcatcggcc
     1681 actagaacct gaaagcccat attacctagc tcagcagctg cagcaagccc agcaggtcca
     1741 gcgcctatga taagaatatc agtgcgaata gatctgcgtt ccgcttgaac aagcggccta
     1801 tcctgagaag gcaacctcgg cagtccatga attgtgcgta catccattcc ttgggccaaa
     1861 ggagttacgc atgacttctt tggaatgccg tcgatcaaca tcgtgcattg tgagcattga
     1921 ccatttgcgc agaatagtcc ctgaggaaag tcatcccgaa agtgatagga aaatacatgc
     1981 ctatccgctg atattaaagc agaagatact ggttctccct cataaccagt tatttcctct
     2041 ccatcgaaag agaatttaac cagagtcaga ttcttttttg cagtgagtat tggatggtgt
     2101 tctattcggt tcatatttca ttcaagccta tgattgaatt ccaaagctgt caataaacat
     2161 actgctacta gaaagcagac tatcgatgat attctgctta tgctatatta aaaaaagtca
     2221 aatgacagca acaccataaa ttaaatcaaa acgaaagagg aattattgcc caaatggatg
     2281 aatttcgcgg caaatatatc accataatgg gacttggcct tcatggtggc ggaatcgcga
     2341 gcgcccgatt ctttgcaaaa gctggagctg aagtcactgt cacagatctt cgcgatgaaa
     2401 aaactctttc cccagctata catgaacttc agggccttcc gatccgatat gttctaggtc
     2461 gtcatgaaat agaagatttt gcccatgcag acttggtaat aaaaaaccca gctgttcgcc
     2521 gtgattcgcc ctatctcgca tatgcaaaga gaatagaaac cgatatttct atatttctgc
     2581 gctattctaa atcgcctctc gtcgcggtca cgggatcaaa aggaaaatcg accatagcat
     2641 ccgccatttg gcatgttctc ttccagaaca ataaaaaggc cctactggga ggaaacatca
     2701 ctgtctcgcc tctcgatttc ctagacaaga cagacgcaca agtccctgta gttcttgagc
     2761 tttcaagctg gcaacttgga gacttgcgag gcacaggttt attaaaacca tctgtagccg
     2821 tgctgagcga tattctgccc gatcatctta actattatga ttccatggac gcttatgtct
     2881 ccgacaaacg tgtaatctat gaatcacagg atgagaattg ctggacagta tgtaatgcag
     2941 atcaaaaatg gggacatgct tttgcagaag aaactctggc gcaagtcctc tggtacagtg
     3001 aaagacctct cgaggattca gtaggaaaaa acgctttggg tggttggctc acgaaaagca
     3061 agaccaaatc aaattattcc gatgattctg gtccttctta ctggtcttta gatcttctcg
     3121 aaggctatgg cagattcagc ctcgatgggc aagaagagct gctggtgcca agccaggttt
     3181 tagtacctgg tcgccatcaa aaaaagaata ttctggcagc tgctgttgca gcacgggcct
     3241 ttggtctttc tccggaaggt atcatgtccg cactcgcaag ctttcctgga gtgccacata
     3301 ggcttgaatt cgtaggctcc attcgaggca taaaatggta taacgacagc acggcaacta
     3361 ttcctgatgc gacaattgca gccattgata gcttttcgag ccccattgtt ctaatagccg
     3421 gcggaagcga taaaatgagc gatttttcgg cttttgccca aaaagcaaag aacctaaaag
     3481 cagtaattct tctagcgggg agtggaacag atcgtcttat tccaatcctc gatgtgaata
     3541 agatagccta caatggacca tttttttcga tgcatgaagc agttcaagct gcagacaagc
     3601 aggcggatcc tggcgatatt gtaattcttt ccccgggctg tgcgagcttt ggtatgtttc
     3661 tgcatgagtt cgcaagaggt gatgctttca aaagagaagc cctaatgctt tttcgccaat
     3721 atgcagaaca gggctctacg gcacaaaagc gatagcggca tatccaacaa atgatcgatc
     3781 aggatggatg tcgatactga gcttatgccc aattagcagg ctatgcctgg ctcctgcatt
     3841 gagagcgaaa gagatggctg cagcaacagc tcctgaggag caagcagaaa aattattcct
     3901 ggcatgtttc aataccttgt ccgcatccat tgcaagcgct gcgcttatga aacccttatc
     3961 gttttcattt cgaacccatt gaacagctgg ctctcctaga cctctcggca taaaaccata
     4021 attagggcca tagtgggtta ggtcggtgga agcaatggca acgagttgct ttttgcagga
     4081 agatgctgcc ctgaagagcg cagatccgag ttctctggcc ttgtaatccg gcggaacgcg
     4141 aagccataga attcgagcat ttgggaatcg aaacccggca agtggcaaca atatttcgac
     4201 ggagttatct atgtctcgat ctggagccat ttcttgaatc ccaacgttct tcaattctgc
     4261 ctttagggaa ttaagaactt caatatcgtt ctctgcatct ctaacagtac agtcaaaacc
     4321 atcttcatct ccatatagga tgggctctcc gttacctaaa tgacctccga gaattgccac
     4381 tgtatcgcaa tctctcaagg cccagactgc cctggcagcc aaatccgcag aaaaatacca
     4441 tccagcatgt ggcgcaacga cggcataggc cgatagatta ttctgagatt tggtccagtc
     4501 agcaacaaga gaactgattt ccgctgaatc ttcaggatac caacccgaag gcaggtatct
     4561 ttttctaatc ctgcgatttg tagtcatggc ttctatcctc ctagcggcaa tcccatgcat
     4621 tttatgcatt tgaatcaata aacaatactc tccgactggg caggtttttg ccgataatga
     4681 tattatatac tgcagggatg gaaaaatgaa acgaacattg cgtataatct tgttcattgg
     4741 gacttttgtg cttttagctc tcattgtagg atatgtcgcg tggaagatcg gtgagatgcg
     4801 ccttctggga gaacagagcg catcagctga atattccatc ctgcgaaatg cagcatctct
     4861 cataacaagt gaagaagatc ttggcgatca attcgtaaga gataggctca aatcactata
     4921 taacgcaagc acacacctcc tggccgttca agttcttgac cgaaacgggc ttgttctttg
     4981 gaaaatgccc gatgagtccc cctacttcgc ttcgccagcc acaaatcctg gtagcgtatt
     5041 tcgtgctcca ggtatgtcga caataatcta catgacacca cttcccgacg gcatgaaact
     5101 gatggcactc tattcaatct tatctcaaag agatatttcg aatattctcc ttgttccagc
     5161 tatcatactt gctgcatgga ttgtgcttct tgtggtattg caatttgcgt taaaggagag
     5221 acctatagag gcttcagaga taatcggcac agaaagaatg cccgaaactc ccgaaaagcc
     5281 tcagtatcac gaggagccat tagagagtgt ttcctattct gaagaagctc ttcttactat
     5341 ttctgatgaa gcctcttcta tagcagagga aagcatagaa aaccatgcgt ctctggaaaa
     5401 tatagaaaac atagaagaaa acatagaaga agaagcagag gaaatcacta ataagaaagt
     5461 ggaaattccg gtatttccac aagctcctca acctgaaccc gaaacgattc cgatgtctat
     5521 ctcatcatct gatgatttcc tgcctggaga attcgtcgct cctgcgccac agatcgaaga
     5581 ggaagcagaa gaagcaccgc aacctgagat ttttccatcc acactttcac tcgctgaatt
     5641 ggaacgcagc cttgatatag agcttcgacg cgagaaagag acgtctctgc tccttgttca
     5701 ctgtctgctt tctggtgagc tggatccaag tgctttagcc ttgggagtta caattcgcga
     5761 ctattttgca tcggattcgc ttgtcttcga gcttgggaaa ggctgttatg cggtggttct
     5821 gcctggcatg gatgcgggtt ctgcgctcaa ggttgcggtg gatctcgacg acgtgctcac
     5881 aactaccgca agtctctata aagatctatc ggaagaacca cccttttatt ttggattaag
     5941 cgcccgatac aatcgcacga tctcccctga tcgcctatat agagaggctt ttgccgcgct
     6001 caaggaagca caggagagaa gcggttcgag aatcctggca tttaagcctg tcagctctgt
     6061 agcatagagc agtgatatac gctaatcgac aaactcgata ggaaagcgca tcctatcctt
     6121 tgttagcacg gtatcaggaa atacctcgcg cgcctcatcg agcaatattt tgagctctcg
     6181 atcggcatat cgcggactat agtgaattaa gcccagcttc tttatgttac ctgcatctcg
     6241 agcaatcaga gcggcttgac gggcggtcat atgccgcttg tcgactgcgc tttcgataag
     6301 cgcgtgctcg aacatccctt cgcatataag aagatccgaa tttgatacct cttgagcaat
     6361 tt
//
LOCUS       DAOMZL010000087         6351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_11486_length_6351_cov_13.057814, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000087 DAOMZL010000000
VERSION     DAOMZL010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11486_length_6351_cov_13.057814"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="PLQ30_06915"
     CDS             complement(<1..1291)
                     /locus_tag="PLQ30_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPW02213.1"
                     /translation="MKLKLRTQFAILTMAVTLFPLVFGLLFFTGQQAKRDPRVTTRLF
                     IDEVAKEWILDNNISLEDIRSAGERVGLPIMDAALVDPDGTVRVSSFRDLPVGIRLEV
                     TDIARPPLLPYGKPKPELRFLPIDANLEKSPLLIFDIQPFLSREDIRNRNFMLVTSLT
                     LGAFIVAGFMSFLILRSTEKAINNLIEDTAIVAKGKLDHEVKGSGALELRSLADSINH
                     MRITLRDMISRRMNMLIGVSHDLKTPIALIQGYTDALSDNMASDEATRIHYLEIIREK
                     SAQLEDLVSELIEFMKIDDLNMRQEAVDLWPLLMSLGKRFEEDARLLGLELLYGFGQV
                     DSALSKEELKDLPRVRMNRALFERAVENLISNAFRYTGAGGHVELALDIKNGQPVLTV
                     ADNGPGIPEEDLPYIFDAFYRGTHSRKEAGHGFGLTIV"
     gene            complement(1291..1995)
                     /locus_tag="PLQ30_06920"
     CDS             complement(1291..1995)
                     /locus_tag="PLQ30_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW02214.1"
                     /translation="MHGRILIVEDTKELAELYKLYLQNEGFECKLAFDAESALPIAQK
                     EEWDLIVLDLNLPGMDGFQFLEQLRKTKSMPVMILTAREADEDVIFGLGVGADDFVTK
                     PCPPKVLAARIRAHMRRTATLQMRQNGSIVRFGPYELDIEGMYLTKNAEPVSLSAREF
                     NILKELVSNPGRPFTPEDLYTRVWGQEYGDISAIGVYIQRLRKKLEDDPANPYYIQTQ
                     YGMGYRFNPEAFVEKA"
     gene            complement(2064..>2391)
                     /locus_tag="PLQ30_06925"
     CDS             complement(2064..>2391)
                     /locus_tag="PLQ30_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02215.1"
                     /translation="GNNLTRVTGSVYALANFSDGSLRFNPQLKVQVGDEGGKVSFTIS
                     DLTSIGDVGSEYAKNGNITTPALTVSLLNSQVSLQASVPIKLNADYSVKKVQTKFSIL
                     WNAIEF"
     assembly_gap    2392..2401
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2402..3694)
                     /locus_tag="PLQ30_06930"
     CDS             complement(<2402..3694)
                     /locus_tag="PLQ30_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02216.1"
                     /translation="MKTRIIKSLLFLIMTTLFLFSKSSIWAQSSSSSSNLKPPEVIDE
                     NELFGGPKDIVTVIDPNVASAGTVQLVEDTKTYPVFLISGSAGAGLYGNYTPLGGSSS
                     DKQTLYGAVDISDLEFDYLPAKNLHFSLSTNLLAVPNQILAASATAYADLRQSDLVRL
                     YVSGSFNYDPSSYLSTNKEYESPTLSLDELFVDTHIEETLFFRLGKQRISWGVGNWFK
                     PSDVLSLSAIDPDDPTAAREGPFAFKVDVPMKLNHLMVYIVPPISENNAASSSAASKY
                     DLVVNGWELSFGAYARADMLARPRAIFSFTGAIGSFDVYGENVLLYGSDRIYARESST
                     PGTYEAYSIENKPFFQSTLGVKYSKSYSGGLSYSIHVQGYYNGMGYADSSIMQNAAAT
                     AAITEAIEDDYPTAYRYSDSSTDAGMWYLAGSASVSGRF"
     gene            complement(3705..4622)
                     /locus_tag="PLQ30_06935"
     CDS             complement(3705..4622)
                     /locus_tag="PLQ30_06935"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPW02217.1"
                     /translation="MLRFYEKKTKYFPSKSIRDISLVILGLVLVLCAQAQGSPPPGGP
                     KGGGGPMAGIGLPLGFLEEDKNYKFPDDITKYTAKDFLMLVRRTDIRSSFYNSDMSAT
                     ITTVNVSPTRGTFVTKEQIFRRDKDDAFLMLTLEPESRKGQGMLRVNNNMWRYDPTSR
                     KFTHTTLKDTYENTTVTNNDFRRWQRSIDYSVEKLSTGTLGNYKVIIGELRANNDEVP
                     FPYIKMYVEADRKIVLKVEEYSLSMKLLRTAYYTQYVQIGDSFVPTVQIFQDGLVPEK
                     RSQVTYTNISTKPLPDYYFTKEYLERVSQ"
     gene            complement(4664..5971)
                     /locus_tag="PLQ30_06940"
     CDS             complement(4664..5971)
                     /locus_tag="PLQ30_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW02218.1"
                     /translation="MHLSNLKLHLKLAARNVRRQPNRTIALGGAVAFSALVMTLISGF
                     VSGMDMAIQDNVTLYSGGHILISGYTPSWSGRLQNRFIDDEIAKTVESAVTDIRTISP
                     LAQARATIVFGTREIQLTLRGIDWTKDELYHDSLLLSSGDWKTLNEQDRSMLMSSQTA
                     DRFGLSIGDQVVVRLTTASGQQNVTDYAVSAIYDDAASGGMTTAFVSFGNLIADLNMK
                     PNEQQQIAIFLKDSTDAERAAKAIAEALTEKGYSIASGTSGSEQATQRRMNSGKVVYS
                     ISTVQELAGEIGSALGSIRWIGYAVFILMLLVSATGIANSYQMVLLERTKEIGMLRCI
                     GFKKKDVFASFISEGILLAGAAAIAGILCALPIGLGISFIPFDPHGDFGAALVKGHLR
                     FVPTLGQLLIILVFIMIVAVVAVFIPARKAAEVVPVEALRITA"
     gene            complement(5974..>6351)
                     /locus_tag="PLQ30_06945"
     CDS             complement(5974..>6351)
                     /locus_tag="PLQ30_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW02219.1"
                     /translation="MIMMNVLVISVNERLHEIGTLRAIGAKRHVIRSMILYETTFMAL
                     IAGAAGLIIGFILLLIMGKVGIRAPNLFFEALFGGEILKPVISIGAAIRAFLWILAMA
                     LAACWYPTRLAVRIEPVVAMRGE"
BASE COUNT         1678 a         1561 c         1410 g         1692 t
ORIGIN      
        1 ttacaatcgt aagaccaaaa ccatgacccg cttctttcct agaatgcgtt cctcgataga
       61 atgcgtcgaa gatgtatggt agatcttctt cagggatgcc cgggccatta tcagccactg
      121 tcaacactgg ttggccattc tttatatcca aagcaagttc tacatggccg ccagctcccg
      181 tataacggaa tgcattggat atgaggtttt ctacagctcg ctcgaatagc gcacggttca
      241 ttctgactct tggaaggtct ttgagttctt ctttcgatag agcagaatca acctgtccaa
      301 agccatatag gagttcaagc cctagaagac gagcatcttc ctcaaaacgc ttacctagag
      361 acattaaaag cggccataag tccacagcct cttggcgcat gttcagatca tctattttca
      421 taaattcgat caattccgag acgagatctt cgagctgagc gctcttttct cgaatgattt
      481 caaggtaatg gatccttgtg gcttcatctg aggccatatt atcgctcagt gcgtcagtat
      541 agccttgaat aagagcaata ggtgtcttta aatcatgact gacgccgata agcatgttca
      601 ttcgcctgga gatcatgtcg cgcagcgtta ttcgcatgtg gtttatgcta tccgcaaggg
      661 agcgcagctc tagcgcaccg cttcccttta cctcgtgatc gagcttgcct tttgcaacta
      721 tagcagtatc ttcgattaga ttattgattg ctttttctgt cgacctgagt atcaagaagg
      781 acataaatcc cgcaacaatg aaggctccaa gcgtaaggct ggtaacaagc atgaaattcc
      841 gattacgaat atcctctcgc gacaagaaag gctgaatgtc gaaaatcaga agagggcttt
      901 tttctaggtt tgcatcgata ggcaagaagc gcagttccgg cttaggcttc ccataaggaa
      961 gaagaggtgg tctagcaata tcagtcacct cgagtctgat tcctactggc aagtccctaa
     1021 aggaagagac cctaaccgtg ccatcggggt ctactaacgc ggcatccata ataggcaatc
     1081 ccactcgctc gcctgcgctc cgaatatcct cgagtgagat attgttgtcc aagatccact
     1141 cttttgcgac ctcatcgata aagaggcgtg tcgttactcg aggatcgcgc ttagcctgtt
     1201 ggcctgtaaa aaaaagcaac ccaaagacta ggggaaaaag tgtcacagcc atggtaagaa
     1261 ttgcaaattg agttcggagc ttaagcttca ttatgctttc tccacaaagg cttctggatt
     1321 gaatcgatag cccattccat attgagtctg gatataatat ggatttgctg gatcgtcttc
     1381 aagcttcttg cgcaatctct gaatatagac tccgatggcg cttatatcac catattcctg
     1441 accccatacg cgagtataga ggtcttcagg agtaaatggc ctgcctggat tgcttacaag
     1501 ttcttttagg atgttgaatt cgcgcgcaga aaggctgact ggctcggcat ttttagttag
     1561 atacattccc tcgatatcta gctcataagg cccaaatctt acgatactcc cattttgtcg
     1621 catttgaagg gtagccgtac gcctcatgtg agctcggatg cgtgcagcaa ggacctttgg
     1681 aggacagggt ttagtcacga agtcatctgc tcctacgcca agcccaaaaa ttacatcttc
     1741 atcagcctct cttgcggtga gaatcattac aggcatggac tttgtctttc tgagctgctc
     1801 aagaaattga aagccatcca tgcctggaag gtttagatcg agcactatga ggtcccactc
     1861 ctctttttgg gcaatgggaa gagcactctc tgcgtcgaat gcgagcttgc attcaaagcc
     1921 ttcattctga agatagagct tatagagctc ggcaagctct ttggtatctt cgacgattag
     1981 gattcttcca tgcatgcccc ctccttcgca agcacccggt gaacgctgcc aggctacttg
     2041 cattgattct ccacttgttg gcatcaaaat tctattgcat tccagagaat gctgaacttc
     2101 gtctggacct tcttgaccga ataatccgca ttgagcttta taggcacaga agcttggagc
     2161 gatacttggc tgtttagaag gcttacggtg agagctggag ttgttatatt gccattttta
     2221 gcatactcag aaccaacatc gccaatagaa gtcaaatcag aaatcgtaaa gctcaccttt
     2281 ccaccttcat ctcctacttg taccttaagt tgcggattaa agcgcagcga accatcggaa
     2341 aaattggcaa gagcatagac ggagcctgtt acacgggtga ggttgtttcc tnnnnnnnnn
     2401 naaagcgccc cgataccgat gcggatccag cgagatacca catgcccgcg tctgttgaac
     2461 tatcgctata cctataagca gtaggataat catcttctat cgcctctgtt atcgctgctg
     2521 tggctgcggc attctgcatt atgctcgaat ctgcataacc cataccatta tagtagccct
     2581 gaacatgtat cgaatacgat aggccgcctg aatagctctt cgagtatttg acgcccaatg
     2641 tagattgaaa gaaaggcttg ttctctatgc tataagcttc gtaggtccca ggtgtcgaag
     2701 actctctagc atagattcga tcggaaccat acaagagcac gttttctcca tatacgtcaa
     2761 aagaacctat cgcgccagta aagctgaaga tcgctctagg tcttgccagc atatcggccc
     2821 gggcatatgc accaaaactg agctcccatc catttacaac gaggtcgtat ttgctcgcag
     2881 cagaagacga agccgcgttg ttttctgaaa ttggcggcac aatatatacc ataaggtgat
     2941 ttagcttcat cggcacatcg accttgaatg caaagggccc ctcccgcgct gcggttggat
     3001 catctggatc aatagccgat aggctcagaa catccgaggg tttgaaccaa ttcccaactc
     3061 cccaacttat acgctgcttg cccaatctaa agaagagtgt ctcttctata tgtgtatcaa
     3121 caaagagttc atccaggctc aaggtaggag actcatattc tttgtttgtc gataaatacg
     3181 acgatggatc gtagttgaag gaacccgaga catagagtcg cacgaggtcg ctctgcctta
     3241 aatctgcata cgcagtggca gacgcagcaa gaatttggtt gggcacagct agaagattgg
     3301 tggataagga aaaatgcaga ttcttagctg gaagatagtc gaattcgaga tcggaaatat
     3361 ccaccgcccc atagagcgtc tgtttatccg aggaactgcc tccaagtgga gtatagttgc
     3421 catatagacc agctccagct gatccactga tcaagaaaac tggataagtc ttggtgtctt
     3481 ccacaagctg cacagttccc gccgaagcga catttggatc tatcaccgta acaatatcct
     3541 tggggccacc gaagagttcg ttctcatcta tcacttcagg cggcttcaag ttggatgaag
     3601 agcttgagga ttgcgcccag atacttgatt tgctaaaaag aaagagagtc gtcattataa
     3661 ggaataacaa gctcttaatt attcttgttt tcattcctct actcctactg gctcacccgc
     3721 tcgaggtatt ccttggtaaa gtaatagtcg gggagcggct tagtggaaat attggtatag
     3781 gtaacctgag agcgcttttc gggcacaagt ccatcctgaa aaatctgcac cgtaggcaca
     3841 aaggaatctc ctatttgcac atactgagtg tagtaggcgg tgcggagaag tttcatagag
     3901 aggctgtatt cctcaacctt aagcactatc tttcggtctg cttccacata catcttgatg
     3961 taagggaatg gaacttcgtc attgttagcc cttaattctc cgattatcac tttgtagttt
     4021 ccaagagtac ccgttgagag cttctccaca gaataatcaa tcgagcgctg ccagcgacgg
     4081 aaatcgttat ttgttactgt cgtgttctca taggtgtctt taagggttgt gtgagtgaac
     4141 tttcggcttg tgggatcgta gcgccacata ttgttgttca ctcgaagcat accttgcccc
     4201 ttacgggatt cgggctcgag ggtgagcatc aagaatgcat catctttatc tcgcctaaag
     4261 atttgttcct tggttacaaa ggtaccgcga gtcggactaa cgtttactgt cgtgatggta
     4321 gcgctcatat cgctgttgta aaagctcgag cggatatcgg tcctgcgaac gagcataaga
     4381 aagtctttcg ccgtatattt tgttatatca tcaggaaatt tatagttctt atcttcttct
     4441 aaaaaaccaa gcggcagccc aataccagcc atgggtcctc cgccaccttt cggtccgcct
     4501 ggaggtgggc tgccctgagc ctgcgcacac aaaacaagaa caagccctaa gatcacaagg
     4561 cttatatccc taatgctttt tgatgggaag tacttcgtct ttttctcgta gaatcgcaac
     4621 atcaatcgct cctcatttta gcgcaaccgc aatattgcaa gcattacgct gtaatcctaa
     4681 gtgcctcgac gggcactact tcagccgctt ttcgagctgg tataaagact gcaacaacag
     4741 ccacaatcat gataaatacc aagattataa gaagctgacc cagggtcggg acgaaacgca
     4801 aatgtccttt caccagggct gctccaaaat ctccatgggg atcgaaggga ataaagctaa
     4861 tacccaggcc tattggcaga gcgcatagaa ttcctgcaat cgccgccgct cccgcaagta
     4921 aaattccttc ggatataaaa gatgcaaaga catctttttt cttgaaacca atgcatcgga
     4981 gcataccaat ttccttagtc cgctcgagga gcaccatctg atatgaattt gcaatccccg
     5041 ttgcgcttac aagcagcata agaatgaaca ccgcataccc aatccagcgt atggagccaa
     5101 gagcggagcc tatttctccc gcgagctctt gtactgtaga gatggagtag actacctttc
     5161 ctgaattcat cctgcgttga gttgcctgct cgcttccaga ggttccagag gcaatgctat
     5221 atcctttttc ggtaagcgct tcggcaatgg ctttggccgc gcgttctgca tcggtgctgt
     5281 ccttgagaaa aatcgctatt tgctgctgct catttggttt catattgaga tcggctatta
     5341 gattcccaaa agaaacaaaa gcagttgtca tcccccccga tgcagcatcg tcatagatcg
     5401 cagaaacagc ataatccgtc acattctgct gaccggatgc cgttgtcagc ctcacgacca
     5461 cctgatctcc aatcgaaagg ccgaatcgat ctgcagtctg agagctcatg agcatgcttc
     5521 tatcttgctc gttgagcgtt ttccaatctc cgctcgataa gaggagagaa tcgtgatata
     5581 gctcatcctt tgtccaatcg ataccacgga gcgtaagctg tatttctcga gttccgaaaa
     5641 ctatcgtagc tcttgcctga gcaagtggag aaatcgtcct gatatctgta acagcagatt
     5701 ctacagtttt tgcaatctca tcatcgataa atcgattctg aagcctgccg ctccagctag
     5761 gagtatagcc cgaaatcaat atgtgccccc ctgaatagag agtcacattg tcctgaattg
     5821 ccatatccat cccgctcaca aacccagata taagagtcat aactagcgca ctaaaggcca
     5881 ccgcaccccc aagggcaatc gtcctattgg gctgtctccg aacgtttcga gccgcaagct
     5941 tcaaatggag cttaagattt gaaagatgca tattcattct cctctcattg cgacaactgg
     6001 ctcgattcgc accgctagcc ttgtcggata ccagcaagca gccaaggcca tagccagaat
     6061 ccaaagaaac gcgcgaattg ccgctcctat cgatataact ggcttcaaga tttctcctcc
     6121 aaaaagagct tcgaaaaaga gatttggcgc tcttatgccg acttttccca taataagaag
     6181 caagataaat cctataataa ggccagctgc accagcgatc agcgccatga atgtggtctc
     6241 atacagaatc atgctgcgaa ttacatgccg cttggctcct atggctctaa gcgtgccgat
     6301 ctcgtggagg cgttcattga cggatatgac aaggacattc atcattatca t
//
LOCUS       DAOMZL010000088         6089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_12170_length_6089_cov_15.167053, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000088 DAOMZL010000000
VERSION     DAOMZL010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12170_length_6089_cov_15.167053"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2120
                     /gene="cas3"
                     /locus_tag="PLQ30_06950"
     CDS             <1..2120
                     /gene="cas3"
                     /locus_tag="PLQ30_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HPW02220.1"
                     /translation="AFNSASWAYAAGFTHDIGKAASIWQHYLRVKSGYDDEAHLEIKA
                     GKQDHSSPSAKFVEEQFGKATGRILSYCIAGHHSGLPDWIGSPSSLSFRLQNIKTCSI
                     PEEYRKDPRFSGSFVPPWKFDNRSLDMSLWIRMIFSCIVDADFLDTESYMEPDNKKTR
                     GDYLSLAELLIQFNSYMDKMIKKTIEEENTIVNQARQKILVECRSAAKSKAGFFSLTV
                     PTGGGKTLSSMAFALEHAVKFGKERIIYVIPYTSIIEQNADVFRKVLGEEQVIEHHSN
                     LDENDSSPKSRLAAENWDAPIIVTTSVQFFESLFAAKPSRCRKLHNIVNSVVVLDEAQ
                     LIPTGYLEPILATMKLLCEHYKVTFVLCTATQPAFEAQDDYPQFPGLPRGSVREIIQD
                     VPRLYRELRRVEVDPVDTNTTRRWEEIAEELKSFERVLCVVSDRRSCRELHALMPKGT
                     YHLSALMCAQHRSDCIAEIKERLKTTEPVRVISTQLVEAGVDIDFPVVYRAMAGLDSI
                     AQSAGRCNREGKLKTNGGLGKVVVFTPQRKPPAGILRKASETTARLLDSGLKDPIDHA
                     AFSQYFSELYWKVNSLDEKGIMKMLSPDMQDCGIQFRSAAEAFHIIDDAMQKTVLVPY
                     GEGEKFIAELKAKGPERWLLRKLQRYSVTIYNDQFYALLNRGSIEEVSPGLFALTCKI
                     EYDDVVGLLIDEMPNDPKSFIAI"
     gene            2133..2864
                     /gene="cas5c"
                     /locus_tag="PLQ30_06955"
     CDS             2133..2864
                     /gene="cas5c"
                     /locus_tag="PLQ30_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HPW02221.1"
                     /translation="MKDWCLEVWGEYACFTRPEMKVERVSYDVMTPSAARAIFEAILW
                     KPAIKWHIRQIEVLRPIRWISVRRNEVGKIENSPTQAHMEGRSSDPMGFYIEDCRQQR
                     AGLFLRDVRYRIHAWFEFIPPDKRMYNHPSAEEVWADEEEREQVSRADENEAKYAAMF
                     ERRARKGQCFHRPYLGCREFACDFRLIEGGTEKQPPIDKTDELGWMLYDMDYRDPSDP
                     KPTFFRAKIENGIMNTDQRIVEVRG"
     gene            2861..4606
                     /gene="cas8c"
                     /locus_tag="PLQ30_06960"
     CDS             2861..4606
                     /gene="cas8c"
                     /locus_tag="PLQ30_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HPW02222.1"
                     /translation="MILQALKAYYDRMALDPESAIAPEGWEWKEIPFIVVLGNNGDLL
                     QIEDTREGEGKKKRGKLFLVPQSVKKTSGIAANLLWDTANYVFGIVNTEGMDNEQTSK
                     ALIRASEQKQAFVKRITEELPQTPNLKAIITFLTTVAKDRLESENAWQDIYASNSVVS
                     FRFDEEKELYCQSKEVQEVLEQKLSVKEKKDICLVSGEKDKIAELHIPIKGVYGAQSS
                     GANIVSFNLEPFRSFNKKQGENSPVGESSMFAYTTALNTLLNRQSSQHLLIGDASTVF
                     WSEKRTHFESDFASFFKEPDKDNPEAGIQKIRNLFEAPLTGGYLDDDSDTKFYVLGLA
                     PNAARIAVRFWEVGTISDFAEKIRQHFEDLNIVKPSSEPPYYSIWRILVNIAIQDKSE
                     NIPPNIAGDFMRSILDGTPYPATLFQAVLRRIHSDTENRVKPVRAALIKAYLNRYLRA
                     HPNQKEKEVEMGLDTEQPSIGYQLGRLFAVLEKIQEEANPGLNATIRERYYGAASSSP
                     VTVFGTLMRLKNHHLAKIDNKGRVVNLERLIGEIVGHIGDFPSHLDLHEQGKFALGYY
                     HQRQDLFTKKENDNQ"
     gene            4631..5509
                     /gene="cas7c"
                     /locus_tag="PLQ30_06965"
     CDS             4631..5509
                     /gene="cas7c"
                     /locus_tag="PLQ30_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HPW02223.1"
                     /translation="MDLDKRYDFVLFFDVKDGNPNGDPDAGNLPRVDAETGNGLVTDV
                     CLKRKVRNYIGLLKNEQPPYEIYVKEKAILNNQNARAYQALNIDLSKDEGKRKGGDKI
                     DEARAWMCKNFYDVRTFGAVMSTGINCGQVRGPVQLTFARSVDQIVSSEHSITRMAVA
                     TEAEAVKQGGDNRTMGRKFTVPYGLYRAYGFVSAPLAKQTGFSQEDLDLFWEALQNMF
                     ENDRSAARGLMGARRLIIFGHSSLMGNASAQELFDRVKVERATDPTKPARDFSDYRVT
                     LDGQELEEFKTVVSVQ"
     gene            5689..5967
                     /locus_tag="PLQ30_06970"
     CDS             5689..5967
                     /locus_tag="PLQ30_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02224.1"
                     /translation="MCSDYRHRGLDIGPALAQRSVFLFGPRQTREFLRLCFHILIDTM
                     LGYELSSYTSTVERKARESRPRKVDGIDILPWREFLGRLWAGDIVAGG"
BASE COUNT         1775 a         1290 c         1487 g         1537 t
ORIGIN      
        1 aagcattcaa ctctgcttct tgggcatatg cggctggttt tacacatgat ataggcaaag
       61 cagcatctat ttggcagcat tatttaagag ttaaaagcgg ctacgatgat gaagctcacc
      121 tagagattaa agcaggcaaa caagatcatt cttcgccaag tgcaaaattc gttgaggaac
      181 aatttggcaa agctacaggc cgaatattat catattgcat agctggccat cattcagggt
      241 tacctgactg gataggttct ccgagctcat tgtcatttag attacagaat ataaaaactt
      301 gcagcattcc tgaggaatat aggaaggatc ctaggttttc tggatctttt gtccccccat
      361 ggaaatttga caatagaagc ctcgacatgt cgctttggat aaggatgata ttctcgtgta
      421 ttgtggatgc agattttctt gatactgaat cctatatgga accagataat aaaaaaacga
      481 gaggggacta tttgtcttta gctgaattat tgatccagtt caattcatat atggataaaa
      541 tgataaaaaa gactattgaa gaagaaaata caattgtaaa tcaagcacga cagaaaatac
      601 ttgttgaatg ccgttcagca gcaaaatcga aggcaggatt cttttcctta acagttccaa
      661 caggcggagg taagaccctt tcgagcatgg cttttgcgct tgaacatgct gtgaaatttg
      721 ggaaagagcg tattatttat gtgatcccat atacaagcat tattgagcag aatgctgatg
      781 tatttcgaaa ggtgcttggt gaggaacagg tcattgagca ccattccaat ttagatgaaa
      841 atgattcaag tccaaaatca cgtttggcag cagaaaattg ggatgctccg attattgtaa
      901 ctacttcagt tcagtttttt gaatcgcttt ttgcagccaa accaagtcgg tgccgcaaat
      961 tgcacaatat cgtaaacagc gtagttgtgc tcgatgaggc gcaactcatc ccgacgggat
     1021 atcttgagcc aattctcgcg accatgaagc ttctctgtga acattacaag gtaactttcg
     1081 ttttatgtac ggccacgcag ccagccttcg aggctcaaga tgattatcct cagtttcccg
     1141 gtctccccag aggaagtgtc agagagatta tacaagatgt ccctaggctc tatcgcgagc
     1201 taaggcgggt tgaagtagat cctgttgata caaacacaac cagaagatgg gaagaaatcg
     1261 cggaagaact aaagtccttt gagagggtcc tctgtgttgt ttcagacaga agaagctgca
     1321 gggaacttca tgcccttatg ccaaagggta cctatcatct ctccgcattg atgtgcgccc
     1381 aacaccgaag cgactgtatc gccgagatca aggaaaggct gaaaacaaca gagcctgttc
     1441 gagtgataag cacacaatta gtggaggctg gagtagatat agatttccca gtcgtttata
     1501 gggctatggc aggcctcgat tccatcgcac aatcggcagg cagatgcaac agagagggga
     1561 agttaaaaac aaatggtgga ctagggaaag ttgttgtctt cactccccaa cgaaaaccgc
     1621 cagcagggat tttgcgtaag gcaagcgaga cgactgcaag gcttctggat tcaggactta
     1681 aggatcccat agatcatgct gcctttagtc agtacttttc cgaattgtac tggaaagtaa
     1741 attctctcga tgaaaagggg attatgaaaa tgctctcgcc tgatatgcaa gattgtggca
     1801 tacagttccg ttccgccgca gaagcatttc atatcataga tgatgccatg caaaagacag
     1861 ttcttgtccc ttatggggag ggagaaaagt tcattgcaga attgaaagcc aaagggccgg
     1921 agcgctggct tctccgcaaa ctccaaaggt attccgtaac catctataat gaccagtttt
     1981 atgctctgct aaatcgaggt tctatcgagg aagtaagccc cggtctcttt gctcttacct
     2041 gcaaaataga atacgacgac gttgttggac ttctcattga tgagatgcca aatgacccca
     2101 aatcatttat cgctatataa aaaggaggcg acatgaaaga ctggtgtctt gaagtatggg
     2161 gagaatatgc ctgcttcaca cggcctgaaa tgaaagttga gcgggtaagc tacgatgtca
     2221 tgactccttc agctgcgcgt gcgatatttg aagctattct ctggaagcct gccattaagt
     2281 ggcacataag gcagatcgag gtattaaggc cgatcagatg gatatctgtg cggcgaaatg
     2341 aggtgggaaa aattgagaat agccccacgc aggcgcatat ggaaggaaga agcagcgatc
     2401 ctatgggctt ttatatcgag gattgccgac aacagagagc cggacttttt ttgcgtgatg
     2461 tgcgttatcg catccatgcg tggttcgagt tcattcctcc ggataagcgc atgtacaacc
     2521 atccgtcagc agaggaagtt tgggctgatg aagaagaacg cgaacaggta tcaagagcag
     2581 atgaaaatga agcgaagtat gccgctatgt ttgagcggcg ggctcgaaaa ggccaatgtt
     2641 tccatcgccc gtaccttggt tgcagagaat tcgcctgcga cttccgcctt attgagggcg
     2701 gaactgaaaa acagccaccg atcgataaga ccgatgaact cgggtggatg ctctacgaca
     2761 tggattacag ggatcctagt gatccgaagc cgaccttttt ccgtgcgaag attgaaaatg
     2821 gcattatgaa cacggatcaa cgcatagtgg aggtacgggg atgatactcc aggctctaaa
     2881 agcttactat gatcgtatgg cacttgatcc cgaaagcgca atagcgccgg agggatggga
     2941 atggaaggaa atcccattca ttgttgtcct cggcaacaat ggagatcttc tgcagattga
     3001 ggatacgaga gaaggtgaag ggaagaaaaa gcgtggaaag ttgttcctgg ttccacagag
     3061 cgtaaaaaag acttctggta ttgcggcaaa tcttctatgg gacacagcaa attacgtgtt
     3121 tggtattgtg aacactgaag gcatggataa tgaacaaact tctaaagcct taattcgggc
     3181 atctgagcag aaacaagctt ttgtcaaacg tataacagaa gaacttccac agactcctaa
     3241 tctaaaagcc atcatcacat ttcttacaac ggttgcaaaa gatagacttg aaagcgaaaa
     3301 tgcatggcaa gatatttatg cctctaattc tgttgtaagt ttccgctttg atgaagaaaa
     3361 agaactgtat tgccaatcaa aagaggtaca ggaggtcttg gagcaaaagc tgtctgtaaa
     3421 agaaaaaaaa gatatctgcc ttgtatctgg ggagaaagat aaaattgcgg aattgcatat
     3481 ccccatcaaa ggagtatatg gtgcacagag ttcaggagcg aatattgtct catttaattt
     3541 ggagcctttt cgatctttca ataaaaaaca aggggaaaat tctccggttg gagaaagttc
     3601 aatgttcgcc tatactactg cacttaatac actactaaac agacaatcat cacagcatct
     3661 tctgattggc gatgcctcta ctgttttctg gtcagaaaaa aggacccact tcgaatcaga
     3721 ttttgcctct tttttcaaag aacccgataa agacaatccc gaggcaggaa tccagaaaat
     3781 tagaaatctc tttgaagcac cgctgacagg aggttacctt gacgacgaca gcgatacaaa
     3841 gttctacgtt ctcggccttg cgcctaatgc ggcgcgaatt gcggtaaggt tttgggaggt
     3901 aggtaccatt tctgattttg cggaaaagat ccgccaacat tttgaagacc tgaacatcgt
     3961 aaaaccatct tctgaacctc catactattc tatatggcga attcttgtga acatcgccat
     4021 tcaggacaaa agcgagaata ttcctcccaa tatcgcaggg gactttatgc gctcaatctt
     4081 ggacggcacg ccttatcctg caacactttt tcaggccgtt ttgcgccgca ttcatagcga
     4141 tactgagaac agagtaaaac cagtacgagc agccttaata aaggcttatc tcaatagata
     4201 tctgcgtgct catccgaacc aaaaagaaaa ggaggtagaa atgggactcg acacagaaca
     4261 gccctcaatt ggctatcagc tcggacggct ttttgcggtt cttgaaaaaa ttcaagagga
     4321 agccaatcca ggcctgaacg caacaatcag agaacgctat tatggtgcgg ccagcagctc
     4381 gcctgtgacg gtttttggaa cgcttatgcg tctcaaaaac catcatcttg caaaaataga
     4441 caataaaggc cgcgtcgtaa atctcgaacg attaatcggt gagatcgtcg gtcatatcgg
     4501 tgatttccca tcacacctcg atcttcatga gcaaggaaaa tttgccctcg gatattatca
     4561 tcagcggcaa gatcttttca ccaagaaaga gaacgataat caataaccaa cacaaggaaa
     4621 ggagattatt atggacttgg acaaacgcta cgattttgta cttttcttcg atgtcaaaga
     4681 tggcaaccct aatggtgatc ccgatgctgg taaccttcca cgcgtggatg cagaaacagg
     4741 caatggactt gttactgatg tttgcctcaa acgaaaagta aggaattaca ttggccttct
     4801 caaaaatgag caaccgcctt acgaaatcta tgtaaaagaa aaggctattt taaataacca
     4861 aaacgcccgt gcctatcagg ctcttaatat cgatctcagc aaggatgaag ggaaaaggaa
     4921 aggtggcgat aaaatagacg aagcacgtgc ttggatgtgc aagaatttct atgatgtgag
     4981 gactttcggc gcagtcatgt ccacaggtat aaattgcgga caagtcagag ggcccgtgca
     5041 attgacattc gctcgctctg tcgatcagat tgtttcatca gaacatagta taacgcgtat
     5101 ggccgtggcg actgaagcag aggcggtgaa gcagggtggc gacaatcgca ccatgggtcg
     5161 taagttcaca gttccatatg ggctctatcg cgcctatggc tttgtctcag cacctctggc
     5221 gaagcaaaca ggtttttctc aagaagacct cgatcttttc tgggaagcac tccagaatat
     5281 gttcgagaac gaccgttctg ccgcgcgggg gcttatggga gcacgcagac tcattatctt
     5341 cggacactcc tcgctcatgg gcaacgcgag cgcccaggaa ctcttcgatc gtgtgaaggt
     5401 ggagcgcgcg actgacccga caaagccggc cagggatttc tcagactaca gggtgactct
     5461 ggacgggcaa gaactggagg agtttaagac tgtagtgtca gtgcaatgat aggaggagat
     5521 atcaatcgct ggtacagtta gtgtttcggt tgcggtgggg ttgatggagt gcgggagaaa
     5581 gctttctatg caatcataag cagcaaccca taagcgccaa ttctgttgtc gtttatcata
     5641 ataatcgcca atcatattgg cgattattat gactttggag agtgatggat gtgtagcgat
     5701 tatcgccaca ggggcttgga tataggccct gctcttgcac agaggtcggt cttcctcttt
     5761 ggcccgcggc agacgaggga attcttacgt ctttgcttcc atattctcat cgatacaatg
     5821 cttggttatg aactttcgtc ctatacctct acagtggaga gaaaggcaag ggaatcccgt
     5881 ccccggaagg tcgacggaat cgatattctg ccatggcgcg aattccttgg ccggctctgg
     5941 gcgggcgaca ttgtggcagg aggttgatct cttgtactct gaggatgatc ttgttccgat
     6001 ctctgcgctc cagcacatac tcttctgcga gcgtcagtat gccctcattt acatcgagca
     6061 ggtctgggag gagaaccgtt tcaccgccg
//
LOCUS       DAOMZL010000089         5912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_12685_length_5912_cov_11.049343, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000089 DAOMZL010000000
VERSION     DAOMZL010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12685_length_5912_cov_11.049343"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PLQ30_06975"
     CDS             <1..712
                     /locus_tag="PLQ30_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02225.1"
                     /translation="PVRMVRGIKYASTNQFVIPFPLRLAIRRDAPLLDSVSPSRLAEE
                     FYKIIATGKSVEIIEALDRYGLLQYLLPEVHASIHGDKAFKEAFLRDLASLDMLAQAP
                     EGTSLLNSATAAEIIQTDKRLISPFLAWFIRQRIKDFAALRDISKEPATFRHEVFSNI
                     RVFLSPLSLPRVALEEAMEQVLQEEDLLPAPPPKRRKHGQRKKNIKPRTESMHLEVEC
                     NDFKIPIEKIFLDNRQNG"
     gene            838..>1759
                     /locus_tag="PLQ30_06980"
     CDS             838..>1759
                     /locus_tag="PLQ30_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW02226.1"
                     /translation="MDLIRFITRRFLRLILTAFIIATIVFFVIRVIPGDPALIIAGID
                     ANPADVQAIRVRLGTDRPLLVQYGDFLWHIARLDFGNSMISGEPVLKLILERFPLTLK
                     LAIFGIAFSIVIAVPLGVLSAVKRWSAWDYFGLVLSQLGMAIPSFWFGIILLLIFAIK
                     IPIFPLFGSETFAHYILPIVSLGFGRAAIILRHTRASMVEELSKEYIITARAKGLTER
                     MVKYKHALKNALLPIITVTSIELGYMLGGAIIIEQVFSMPGLGRLFLSGIYQRDFPII
                     QGGVVFVAILFSSINFIADILYSIANPKIRL"
     assembly_gap    1760..1859
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1860..2579
                     /locus_tag="PLQ30_06985"
     CDS             <1860..2579
                     /locus_tag="PLQ30_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW02227.1"
                     /translation="RTYRGRRIAMIFQEPAKYLNPAFKIGEQIIEMLMLHIKMNKQSA
                     CKRAIELLRLVGLDEKVIDKYPHELSGGMKQRAMIAMAISCDPDFLIADEPTTALDVT
                     LQIQILALLKKLKDMKKLGVLFISHDLHLVSEIADRVSVIYAGRIVESAVKEDLFKNP
                     LHPYTRLLMHSIPDAKKKGSKLATIPGQVPDAAEIGNGCAFANRCPIAKENCFTDIPG
                     YVEYENAHTVACYYAGKIWEI"
     gene            2567..3553
                     /locus_tag="PLQ30_06990"
     CDS             2567..3553
                     /locus_tag="PLQ30_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW02228.1"
                     /translation="MGNLIESKDITKIYRSRGLGGSKHGFKAVDCVSLTIRKQSIFGL
                     VGESGCGKTTFGRALMYLDPPTSGSVVFDDVPLGSLKPKELRRFRNRMQIVFQDPNSS
                     LDPKMRIYDSLSEGMVNLKIPREQRDRKVNNLINLVGISKTYLHRFPHELSGGQKQRI
                     GIARALSVDPDFLVLDEPTSNLDVSIQAQIINLLLDLKDEFGLTYLFISHDLNLISYM
                     CDEIAVMFKGRIVEQAETEQLISKPSHPYTQRLLSSIPGGPQRIGVSCLNIEDLDEKT
                     IKERQEHEKSHSSCPYYSQCPMGDAECSLSAPSLRELSGGHVVSCHKIRTGK"
     gene            3577..5076
                     /locus_tag="PLQ30_06995"
     CDS             3577..5076
                     /locus_tag="PLQ30_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW02229.1"
                     /translation="MKRSVIVGMLFLTVISGAGGSLFAQGKSSITFGLSGNPDTLDPQ
                     KTAGTLTFQVVKSFYDTLVEPDTTGKLVPALAESWTVSPDGLTWTFKLRQGVVFHNGQ
                     PLTSRDVKATLDRIRDPSFASPRRSEFTAIKEVRTPDPQTAVIVLGAPYAPILANLAS
                     GWGAILPADLIQSGHNFDSEPIGTGPFKFEKWVRDNKISMVRNDKYWMKGLPKLARVD
                     FQIVPERSVQIQGLMSGAIDAVEYVDPEDLPILQSNPNVTIRKDQTSLILVMAMNCSR
                     EPLNDLRVRQAINYAVDKQLVLDVAYGGGKVGSSFMDTGNAYYTDFSYLYPYNPEKAK
                     QLLKEAGIGNRPFTITVPQNYPLHVKAGELIQQMLAKVGMNVKIQLVDWSTWLARIYT
                     EGNFDLTIIGHTGKLDPDGTLAGYGTDKKYVRWINPTCDAKIKEAATISDFAARKKLY
                     TEALEIMAKEVPFVYLGSSYRYVAMRSNVADFRITPNLDSYDFRWTELK"
     gene            5083..5907
                     /locus_tag="PLQ30_07000"
     CDS             5083..5907
                     /locus_tag="PLQ30_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW02230.1"
                     /translation="MFGKSQKILFDLAVFYLLVLIVSAIAAPLITPYIPTEQFLSKRF
                     HPPSFDNIMGTDNFGRDILSRIIYGSRSALIVGIVTVTIALAIGMAIGLLAGMGSKSL
                     DNVLMLFMDSLLSFPTILLAITVVSFLGYGIVQVMLALGIISSPVFARLIRAETLAIK
                     TEGYVEAAKALGTSSFKIAFRHIIPNLLGKVAVQCSLTFGQAIVIESSLSYLGVGTQP
                     PDASWGLMLKDARNYLIQAPWMAIYPGLCLALTVLAFNIIGDSLAESLNPRLTGSS"
BASE COUNT         1612 a         1184 c         1361 g         1655 t
ORIGIN      
        1 tcctgttcgt atggtgcgcg gtattaagta tgcttcaacc aatcaatttg taattccatt
       61 tccattgaga ttggcgattc ggcgcgatgc gccgcttctt gattccgtaa gcccatcgag
      121 gcttgcagaa gagttctaca agataatagc taccggaaaa tcagtagaga ttatcgaggc
      181 attggataga tatggcttgt tgcagtacct attgcctgag gttcatgctt caatccatgg
      241 agataaagct ttcaaggagg cattcttaag agacttggct tcccttgata tgctagccca
      301 ggcgcctgaa ggtacaagcc tactaaacag cgcaaccgcc gctgagatta ttcagacaga
      361 taaaaggctt atatctccat ttctagcttg gttcatccgt cagaggatca aggatttcgc
      421 tgctctcaga gacatttcga aagaacctgc cacatttagg catgaggttt tttcgaatat
      481 tcgggtattt ctatccccgc tcagcttgcc tcgagtggcc ttagaggagg caatggaaca
      541 agtgcttcaa gaagaagatc tgctgcctgc tcctcctccc aaaagaagaa aacacggtca
      601 acggaagaag aatataaagc cgcgcactga gtcaatgcac ctcgaagtag aatgcaatga
      661 tttcaaaatc ccgatagaaa aaatttttct tgacaacaga caaaatggat aatagagtgt
      721 taaccttagg ttttatttta ccatcaccgc agatagaagg gagcattttt attgaaatcg
      781 agcgatatct atataattaa tcgtcatatc gacagatgat attctagtag gccaaacatg
      841 gatttaattc gcttcattac aagaagattc ttgaggctta tactgacagc ctttataatt
      901 gccacgatag ttttctttgt tattcgtgta attccgggag atcctgcatt gattatcgcg
      961 ggaattgatg ccaatcctgc tgatgtgcaa gcaatccggg taaggttggg aacagatcgt
     1021 cctcttcttg tccaatatgg agattttctt tggcatatag cacgtcttga tttcggtaat
     1081 tcaatgatct ctggggaacc tgttctcaaa cttattctag agcgatttcc tcttactctt
     1141 aagcttgcaa tttttgggat tgccttcagc attgtaattg cagtaccact tggtgtttta
     1201 tctgctgtaa agcgatggtc ggcttgggat tactttgggt tggttctttc ccaactagga
     1261 atggctattc ctagtttttg gtttggcata attttgcttt tgatttttgc aatcaagata
     1321 ccaattttcc cgctttttgg ttcggagacc tttgcacatt acattctgcc aatagtctcg
     1381 ctaggatttg gtcgtgcagc aattatctta cgccatacaa gagcatctat ggtggaagag
     1441 ttgtctaagg aatacataat tactgcaagg gcaaaaggcc ttactgagcg gatggtaaaa
     1501 tacaagcatg ctctaaagaa tgcccttttg cctataatta ccgtaacaag tattgagctg
     1561 ggctatatgc ttggaggcgc aattataata gagcaggttt tctcgatgcc gggtttggga
     1621 aggctcttct tatccggcat ctatcagcgc gatttcccga tcatccaagg aggtgtggtt
     1681 tttgtagcca tcctgttctc ttcgataaac tttattgctg atattcttta cagcattgct
     1741 aatcctaaga taaggctaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     1861 gaacctacag aggcagaaga attgccatga tatttcagga gcctgcgaag tatctcaatc
     1921 cagcattcaa aatcggagag cagattattg aaatgctcat gcttcatata aagatgaaca
     1981 agcaatctgc ttgtaaaagg gctatagagc tattgaggtt ggtaggcttg gatgagaagg
     2041 ttatcgataa gtatccgcat gaattatcag gagggatgaa gcaaagagcc atgattgcca
     2101 tggctatttc gtgtgatccc gattttctca tagctgatga gcccacaacg gcgctcgatg
     2161 tgactttaca gattcaaatc ctcgcattgc tgaaaaaact aaaggatatg aaaaaattgg
     2221 gtgtattatt catttctcac gatcttcatc ttgtaagcga gattgccgat cgggtttcgg
     2281 ttatctatgc tggaagaata gtcgaaagtg cagtaaaaga agatctcttc aagaatccat
     2341 tgcatcccta tacgcgtttg cttatgcatt ccattccgga tgccaaaaag aaggggtcaa
     2401 aacttgctac aattccgggg caggttcccg atgcggcaga aataggcaat ggctgtgcat
     2461 ttgcaaatcg ttgtccaatt gcgaaagaga actgctttac ggatattcca gggtatgttg
     2521 aatatgagaa tgcacatacg gtcgcttgct attatgcagg taagatatgg gaaatctgat
     2581 agaaagcaag gatataacaa agatatatcg ttctcgtggg ctaggtggat cgaaacatgg
     2641 ctttaaggcc gttgattgtg tttccctaac cataagaaag cagagtattt ttgggctcgt
     2701 aggagagtcg ggatgcggca aaacgacgtt tggaagagcg cttatgtatc ttgaccctcc
     2761 aacatctggt tcggtggttt tcgatgatgt cccgctagga tctctgaagc ctaaggaatt
     2821 gagaagattt cgaaatagaa tgcaaattgt ttttcaggat ccgaacagct cgctggatcc
     2881 aaagatgaga atctatgata gtctatcaga aggcatggtg aatctaaaaa tccctagaga
     2941 acaacgagac cgcaaggtca acaatctcat caatcttgta ggaatcagta agacatatct
     3001 gcatcggttt ccgcacgaac tctctggagg gcaaaagcaa aggattggta tagctcgtgc
     3061 tctttctgtg gatcctgatt ttcttgtact ggatgagccc acatccaatt tagacgtatc
     3121 cattcaggcg cagataataa acctattgct cgatttaaag gatgaatttg gattgactta
     3181 tctattcatt agccatgatc ttaatcttat atcttatatg tgcgatgaaa tagcggttat
     3241 gttcaaaggc agaatcgtag agcaagcgga aacagaacag cttatttcaa aaccaagcca
     3301 cccatatacg caaagacttc tcagttcaat accaggaggt ccgcagagaa ttggtgtatc
     3361 ctgcttgaac attgaggatc tcgacgagaa gacaataaaa gagcgccaag agcacgaaaa
     3421 atctcactca agctgtcctt actattcaca atgtccgatg ggtgatgctg aatgtagctt
     3481 gtctgctcct tccttaaggg agctttcagg aggacacgtt gtgtcctgtc ataaaatcag
     3541 aactggtaaa tgactcgcat ttctaaggag aaagagatga aaagatcggt gattgtggga
     3601 atgctttttc ttaccgttat atctggagca gggggttccc ttttcgcaca ggggaaaagt
     3661 tctattacat ttggtctatc cggcaatcca gatacgcttg atcctcagaa aacagcagga
     3721 acgctgacat tccaagttgt aaagagtttt tatgatactt tggtagagcc cgatacaacg
     3781 ggtaagcttg tgccggcgct tgccgagagt tggacggttt ccccagacgg attgacatgg
     3841 acattcaagc tacggcaagg tgtcgtgttc cataatgggc agccattgac ctctcgggat
     3901 gtaaaggcaa cattggatcg cataagagat ccgagcttcg cttcaccccg aagaagcgaa
     3961 ttcacggcaa taaaagaagt gaggacaccg gatccacaga cggctgtgat tgttcttggt
     4021 gcgccttatg cgccgattct tgccaatctt gcttctggct ggggagcaat cctgcctgcc
     4081 gatcttatcc agtctggcca caactttgac tctgaaccca tcgggacagg gcccttcaaa
     4141 ttcgagaagt gggttcgcga taacaagata tcgatggtca ggaacgacaa atactggatg
     4201 aagggcttac ctaaattggc aagggttgat ttccaaatag ttccagaaag atccgttcag
     4261 atacaaggat tgatgtctgg tgcaattgat gctgttgaat atgtcgatcc ggaggacttg
     4321 ccaatactcc agtcaaatcc gaatgtcaca ataagaaagg atcagacctc gttgatcctg
     4381 gttatggcaa tgaattgctc tcgtgaacca ctcaatgatc ttcgcgtgcg ccaggccata
     4441 aattatgcag tcgataagca gcttgtgttg gatgtagcat atggcggtgg aaaggtcggg
     4501 tcttctttca tggatacagg gaatgcttac tatacagatt tttcttacct ctatccttat
     4561 aatcccgaga aagcgaagca attactcaag gaagccggaa taggtaacag gccattcact
     4621 ataactgtgc cccagaacta tccgttacat gtgaaagccg gagaactaat tcaacaaatg
     4681 ctcgcaaaag ttggcatgaa tgtcaagata cagctcgtag attggtcaac ctggctggct
     4741 agaatctata ctgaaggcaa tttcgatttg acaattattg ggcataccgg caaactcgat
     4801 ccggatggga cgctggccgg atatggcaca gacaagaagt atgtcaggtg gatcaatcca
     4861 acctgcgatg caaaaataaa ggaggccgcc accatctctg atttcgctgc tcgcaagaag
     4921 ctatatacag aggcattgga gataatggca aaagaggtac cttttgtata ccttggaagc
     4981 tcatatcgct atgtagctat gagatccaat gtagcagact ttaggattac cccaaacttg
     5041 gattcatacg atttccgttg gacagaattg aagtagaaga gtatgttcgg aaagtcccag
     5101 aagattttgt ttgaccttgc tgtattttat ctcctcgtat tgattgtatc agcaatagcg
     5161 gctccgttga taacaccgta cattccaaca gagcagtttc tctctaagcg tttccatccg
     5221 cccagctttg ataatattat ggggacggat aattttgggc gcgatatcct aagcagaatt
     5281 atatatggat ctcggtcggc gctcattgtg ggcatcgtaa ccgtgacaat tgcgcttgca
     5341 ataggaatgg ccatagggtt gcttgcaggc atgggctcga aaagtttgga caatgtgttg
     5401 atgctattca tggatagtct actttcattt cctacgattt tgcttgcgat caccgttgtg
     5461 tcgtttctgg gatatggaat tgttcaggta atgctcgcgt taggaataat cagtagccct
     5521 gtatttgcaa ggctcataag ggcggaaacg cttgcaataa agaccgaagg atacgtggaa
     5581 gctgcaaaag ccctgggtac atcctcattc aaaatagctt tcaggcatat catcccaaat
     5641 cttctgggga aagtagctgt tcaatgttct ctcacctttg gtcaggcgat tgtcattgag
     5701 tcttctttat cgtatctggg ggttggaacc cagcccccag atgctagttg gggcttgatg
     5761 ttgaaggatg cgcgtaatta cctcattcaa gctccatgga tggctatata cccaggtctc
     5821 tgtctggcct tgacagttct tgcattcaat attattggtg attcgcttgc agaatcgctt
     5881 aatccaaggc tcactggttc ttcttgagat at
//
LOCUS       DAOMZL010000090         5850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_12869_length_5850_cov_22.333218, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000090 DAOMZL010000000
VERSION     DAOMZL010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12869_length_5850_cov_22.333218"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..1235
                     /locus_tag="PLQ30_07005"
     CDS             63..1235
                     /locus_tag="PLQ30_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPW02231.1"
                     /translation="MIEGSNEVNTSKKLRYGMVGGGKGAFIGDVHRKAIAMDGLAELV
                     AGSFSQSFENTCETGRNLGLSRDRLYATYVEMAEKEASRPDCIDFVSIVTPNALHYPI
                     AKTFLNAGINVVCDKPLTFEESEARELQEIARQKDLFFCVTYAYTGYPAVKQAREMIR
                     RGYIGDIRFVNAEYPQEWLATATEREGSKQASWRTNPALSGKSNCVGDIGSHAENLVS
                     YITGLKIKSLCARLDAFVPGRVLDDNASIMVEYEGGAKGLYWASQIAIGYDNALKVRI
                     FGTKGTIQWSQENPNYLFVSKLGEPSRLLSRGRDPFYPHPQSYSRVPSGHPEGYFEAF
                     ANIYKTFITALIKKKLGQSLTEEDLDFPTAEEGAIGVRFIGKCVESSQKGAIWLAF"
     gene            1292..2281
                     /locus_tag="PLQ30_07010"
     CDS             1292..2281
                     /locus_tag="PLQ30_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPW02232.1"
                     /translation="MARPVTIFSGQWADLPFDQFCAKVKSFGYDGIEIACWGDHMDVK
                     AAATDPNYVKERKAILEKNGLNCWALGAHLAGQCVGDLWDSRLDGFAPTECKGQPEKI
                     RKWAIQEMKYTAEAAKNMGCGVVTGFMGSPIWKFWYSFPQTPDEMVDEGFKEIVKLWT
                     PILDEFDRCGVKFALEVHPTEIAFDYYTAGRLLKEFGNRPTLGFNFDPSHLIWQGISP
                     HLFIGDYASRIYHVHMKDAAVTLDGKAGILGSHMAFGDTRRGWNFRSLGHGDVDFENI
                     IRELNAAGYQGPLSVEWEDSGMDREYGAKEALDFVREVDFVPSSIAFDAAMKK"
     gene            2281..3777
                     /locus_tag="PLQ30_07015"
     CDS             2281..3777
                     /locus_tag="PLQ30_07015"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPW02233.1"
                     /translation="MHTIIRTEGITKEFSSVRVLNDISVEICTGEVFGIIGENGAGKS
                     TFIKVLSGLYQPTSGKIWFDDKLINMRSPADAKRIGISLIPQEFSLVSDLCVYENIFL
                     GEELVGKHGILDIKAMVNRTRQLLTDLEVSTIDPYAHIQALSVAQKQMVEIAKAIAFD
                     SRLLIMDEPTTMLTSKEIEILFGLIERLKQRGVTIIYISHKLKEVKHICDRVMVLRDG
                     EFICLEDTVNLTIDEMARRMVGRELKDVFPEKKNQPGDVVLSVRNLCVDKLLYDISFD
                     LHASEILGFAGLIGAGRTELIETIMGLRRARSGTIILNGNQLSIQNPWDAVDAGLSYL
                     SEDRQGAGIITSLDVVKNITLASLKHYAKPFIDQQAEENKAKFYIDRFSIKVPSLQAK
                     LEQLSGGNQQKVSLAKSIDVQPKIIIVDEPTRGIDVKSKQDIYIFIHELAASGLSVIF
                     ISSELEEIIGMCDRVLIMRAGQIVGELRSDKLSEEEIMYFATGVKGGY"
     gene            3778..4782
                     /locus_tag="PLQ30_07020"
     CDS             3778..4782
                     /locus_tag="PLQ30_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW02234.1"
                     /translation="MKIPNFKKTSLSKSGPLIALLVLVIISTILYPRFLEIQNLLNIL
                     RQVSYTGIIALGMTFVIISGGIDLSVGSMTALAGAGAILMLNMITNALVGFSPMWLGT
                     FLSILSAFIVSLFIALSAGFLNGFLITKGRIAPFIVTLGTMAIFRSLTLYIGKAGEFR
                     SVNSSYGEMGMSVILGIPLPVWIFFILAMILSFVLNRTRYGRYVCAVGSNEKVARYSA
                     INVDKIRLISYVIVGLMVGITAVLISARLNSLSSTNAGKDYEMDAIAAVVIGGTAMTG
                     GKGSIWGSVIGAIILGIINNMLNMLGISPYLQGLVKGLVIIGAVSIQRGWINNSRSEK
                     "
     gene            4885..5847
                     /locus_tag="PLQ30_07025"
     CDS             4885..5847
                     /locus_tag="PLQ30_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW02235.1"
                     /translation="MKKIVIAFMLLLAIAAVPVFAQKVKIGVSIPTADHGWTGGINFW
                     AQKAIKDWQAKDKNIEFYLVTADSPAKQVNDVEDLMVKGINALVILAHDSAPLTPVVK
                     KAYEKGIYVVSVDRGLTEEVQDVYIAGDNPGMGRASAEWLGKALGGKGDIVVLEGIPC
                     VINSERVDSFKEVMTKKYPGIKILDSQPAYWDTQKGLEIMTNYLQKYKKIDAVWAQDD
                     DVLVGVLQAYKESGRKDIKYFLGGAGSKVMIKKIIDGDPLVQADVTYPPSMIATGISL
                     AVYGSRNQPLPGFYQAKIPSKIILAAELITKENAKDYYQPDSVF"
BASE COUNT         1597 a         1210 c         1473 g         1570 t
ORIGIN      
        1 atgcagtatg tgctgggagt ggagtaattt agtcaattga taatgaattg aggaggtatg
       61 acatgattga aggtagcaat gaggtaaaca cctcgaaaaa attgcgatat gggatggtcg
      121 gtggcggcaa aggagccttt ataggcgatg ttcatcgcaa ggcgattgca atggatgggc
      181 ttgccgagct tgtcgcaggc agcttctcgc agagtttcga gaatacttgt gaaacgggcc
      241 gaaatctagg attgtcgcgc gacagactct atgcgaccta tgttgagatg gcagaaaaag
      301 aagcgagcag acccgattgc atcgattttg tatccatcgt taccccaaac gcactgcact
      361 atcctattgc aaagacattc cttaatgcag gaatcaatgt tgtatgcgac aagccgctga
      421 cttttgaaga gtcagaggcg agggagcttc aggagattgc tcggcaaaaa gatctcttct
      481 tttgcgtaac ttatgcctat actggttacc ctgcagtcaa gcaagcgcga gaaatgatcc
      541 gccgagggta tatcggagac atacgctttg tcaatgctga atacccacag gagtggctag
      601 ctactgcaac tgagcgagaa ggttccaagc aggcttcctg gaggacgaac cctgcacttt
      661 ccggcaaatc gaattgcgtc ggagatatcg ggagccacgc cgagaacctc gtctcctata
      721 taactggtct caaaatcaaa tcgctctgtg cgcggctcga tgcatttgtc cctggccgag
      781 tgcttgatga caatgcatcg atcatggtcg agtatgaagg tggtgcaaag ggattgtatt
      841 gggctagcca gatcgctatc ggctacgaca atgcccttaa agtgcgaatc tttggaacca
      901 agggcactat tcaatggtcg caggagaatc ccaattacct tttcgtatcg aagctcggcg
      961 agccctcacg cttgctttct cgcggccgcg atcctttcta tcctcatccg caatcatact
     1021 cgcgagtacc ttcaggccat ccagaaggct acttcgaagc ttttgcaaat atctataaga
     1081 cctttattac cgcgcttatt aagaaaaaat taggccaatc actaaccgaa gaggatctag
     1141 acttcccaac tgcggaagaa ggcgctatcg gtgtgcgttt tatcggtaag tgcgtcgaat
     1201 cctcacaaaa gggtgcgatt tggctggcat tttagtgagg ttctttagag cttatatgat
     1261 aaataagata tgattttata ggaggatgac tatggcaaga ccagtgacaa ttttctcagg
     1321 ccaatgggca gaccttccct ttgaccaatt ttgcgctaaa gtcaaatcgt ttggctatga
     1381 tggtattgaa atagcttgct ggggcgatca catggatgtc aaagcggcag caaccgaccc
     1441 gaactatgtc aaagagcgca aagcaatcct tgaaaagaat ggacttaatt gttgggcgct
     1501 tggtgcgcat cttgcaggac agtgcgtagg cgacctatgg gattctcgtc tggacggttt
     1561 tgctccaacg gaatgtaagg gacagcctga aaaaatccgt aaatgggcta tacaggaaat
     1621 gaaatatacc gctgaagctg caaagaatat gggatgcgga gtagtaacag gttttatggg
     1681 aagccctatc tggaaatttt ggtattcctt tccacagact ccagacgaaa tggtagatga
     1741 aggctttaag gagatagtaa agttatggac acccattctc gacgagttcg atcgttgtgg
     1801 tgtcaagttt gcactcgagg tgcatccaac tgagatagcg ttcgattact acactgcagg
     1861 tcgcttgctc aaggaattcg gcaaccggcc aactcttgga tttaacttcg atccctcaca
     1921 tctcatctgg cagggtatct cgccgcatct ttttatcggc gactacgcaa gcaggatcta
     1981 ccatgtgcac atgaaagacg ctgcagtgac actcgatggt aaggcgggca tccttggctc
     2041 tcatatggct ttcggagata cgcgtcgtgg atggaatttc cgctctctag gccatggaga
     2101 tgtggatttc gaaaacatta ttcgtgaact caatgctgct ggctatcaag ggccgctctc
     2161 ggtggagtgg gaagattctg gtatggatcg cgaatatggt gcaaaggaag ctcttgattt
     2221 tgtgcgagag gtagactttg taccttcgtc gattgccttc gatgcagcga tgaaaaaata
     2281 atgcatacaa ttataagaac cgaaggcatt accaaggaat tttcctcagt tcgggtcctc
     2341 aacgatatct ctgtcgaaat ctgcacaggg gaggtctttg gcataattgg tgagaatgga
     2401 gcagggaaat ctactttcat caaggttctt tcgggactat accaaccaac atccggcaag
     2461 atctggttcg atgacaagct aataaatatg aggagccctg ccgatgcaaa aagaatcggt
     2521 ataagcctga ttccccagga attcagcctt gtttcagatc tttgcgtata tgaaaacata
     2581 ttcctcggtg aagaacttgt aggaaaacat ggaatcttgg atatcaaagc aatggtcaat
     2641 aggacgcgcc agctcctcac cgatttggaa gtctccacaa tcgatccata tgcacacatt
     2701 caggctcttt cagtagcgca gaaacagatg gtcgagatag ctaaagctat cgcttttgat
     2761 tctcgccttc tcataatgga tgaacctaca accatgctca ccagcaagga gatagagata
     2821 ctatttgggc ttattgaaag gctcaaacaa cgaggtgtca ctatcatcta tatttctcat
     2881 aagctcaagg aagttaagca tatctgtgat cgcgtaatgg tccttcggga tggagagttt
     2941 atttgcctcg aagatactgt gaatctaacc atagacgaaa tggcgcgcag gatggtaggg
     3001 cgcgagctaa aggatgtttt ccctgaaaag aagaatcagc ctggcgatgt agtgctctct
     3061 gtacgaaatc tttgtgtaga taagcttctt tacgacatca gtttcgatct ccatgcttct
     3121 gagatattgg gctttgcggg tctcattgga gcaggcagaa cagaacttat tgagacaatt
     3181 atgggtctaa ggcgtgcacg ctccggcaca attattctga acggtaatca gttgtctata
     3241 caaaatcctt gggatgctgt cgatgcaggg ttaagttatc tctcggaaga ccgacaaggc
     3301 gcaggaatca tcacctcatt agatgtggta aaaaatatca cccttgcatc actaaagcac
     3361 tatgcaaagc catttatcga tcaacaggca gaagagaata aggcaaagtt ctatatcgat
     3421 cgattttcta ttaaagtacc atcgcttcag gcaaagctcg agcaactctc aggtggtaat
     3481 cagcagaaag tctctttggc aaagagcata gatgtacagc ccaagatcat aattgtggat
     3541 gaaccgaccc ggggaatcga tgtaaaatca aagcaggata tttatatttt tattcatgaa
     3601 ttagctgcta gcggtctatc ggtcattttt atatcttctg aattggaaga gataattggg
     3661 atgtgcgata gagtgctaat catgcgagct ggtcaaattg ttggtgagct aaggtcggac
     3721 aaattgagcg aagaagaaat catgtatttt gcgacaggcg tcaagggagg gtattgaatg
     3781 aaaattccaa atttcaagaa gacgtcttta agcaaatcag gccctctgat tgcgctgctc
     3841 gtcttggtga ttatttcgac gatcctctat ccgagatttc ttgaaattca gaatctgctc
     3901 aacattctgc ggcaggtctc ctatacagga ataattgcgt tgggtatgac ttttgttatc
     3961 atatcgggag gaattgatct ttcagtaggc tctatgaccg cattggccgg agcaggtgcc
     4021 attcttatgt tgaatatgat caccaatgct cttgttggtt tttcaccgat gtggcttggc
     4081 actttcttaa gcatcctgtc ggcgtttatt gtttcgcttt ttattgctct aagtgcgggt
     4141 tttctcaatg gctttctcat cacaaaaggg agaatagctc ctttcatcgt aacgcttgga
     4201 acaatggcta tctttaggtc gctgacctta tacatcggaa aagcaggcga atttcgatcg
     4261 gttaactcct cctatggtga aatgggaatg tcggtgatct tggggattcc tctgcctgta
     4321 tggattttct ttatcttggc gatgattctt tctttcgtat tgaacaggac aagatatggc
     4381 cgctatgtat gcgcagtggg ttcgaatgag aaggtcgctc gctattctgc aataaatgtc
     4441 gataagatac ggctaatttc ttatgttatt gtaggcttaa tggttggaat aacggcagtg
     4501 ctcatttcag cgcggctcaa ttcgctgagt tcgaccaatg caggcaagga ctacgaaatg
     4561 gacgctatcg ccgcggttgt aataggcggt actgccatga cgggtggaaa gggttccatt
     4621 tggggctctg tgattggtgc tataattctt ggtattatca ataatatgct taacatgctt
     4681 ggtatttctc cttatctgca gggcctcgta aaagggctcg tcatcatagg tgcagtatca
     4741 attcagagag gatggatcaa taacagccgg agtgaaaagt gagaaatagg agaacgcgaa
     4801 tggaaaaaaa tcgggaatct atcgggtagc cgttggcatt tgcccgattg agaaagaaat
     4861 acgcattgta ttggaggagg cgttatgaaa aaaatcgtca ttgcctttat gctgctgctt
     4921 gctattgccg cagtaccggt atttgcccag aaggtcaaga ttggtgtttc cattcccacc
     4981 gctgaccatg gttggacggg aggtatcaat ttttgggccc agaaagcgat caaagattgg
     5041 caggccaagg ataagaatat cgagttctat ctagtaactg ctgattcacc tgcaaaacaa
     5101 gtcaacgatg ttgaagatct gatggtgaaa ggtatcaatg cgcttgtcat tctagctcat
     5161 gattccgcac ctcttactcc tgtagtaaaa aaggcatatg aaaaaggcat ttacgtagtc
     5221 tcagtagacc gcgggctcac tgaggaagta caggatgtct acattgcagg cgataaccct
     5281 ggcatgggaa gggcttcggc ggaatggctt ggcaaggcac ttggcggcaa gggcgatatt
     5341 gtcgtgctcg aaggtattcc ttgtgttatc aacagcgagc gtgtggatag cttcaaagaa
     5401 gtaatgacaa agaaatatcc tggtattaaa atcctcgact cgcagccagc ttattgggat
     5461 acgcagaaag gccttgagat tatgaccaac tacctacaga agtacaagaa gatcgatgcg
     5521 gtctgggctc aggacgacga tgttctcgta ggtgtgctcc aggcatacaa ggaatctgga
     5581 cgcaaggata ttaagtactt ccttggcggt gcaggcagca aagtgatgat caaaaagata
     5641 atcgatggcg atccgcttgt gcaggcagat gtaacttatc cgccctctat gatagcaacg
     5701 ggtatctcgc tcgctgtcta tggctcgcgc aaccagcctt taccaggatt ctaccaagct
     5761 aagattccgt cgaagattat ccttgccgca gagcttatca caaaagagaa cgcaaaggat
     5821 tactatcagc cggattctgt gttctgattc
//
LOCUS       DAOMZL010000091         5802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_13030_length_5802_cov_15.474508, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000091 DAOMZL010000000
VERSION     DAOMZL010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13030_length_5802_cov_15.474508"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /gene="greA"
                     /locus_tag="PLQ30_07030"
     CDS             <1..422
                     /gene="greA"
                     /locus_tag="PLQ30_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPW02236.1"
                     /translation="IMEVDVPANQKEIAYALSLGDLRENAEYKAAKERQDELNAKVGK
                     LKSELDRAQIIDSDQVDSTSISFGTKIALKNLITGEDEFFTILGPWESDPEHQVISYL
                     SPLGKHLLNHKTGEKLNFTINERKFSYEVVKVEKADF"
     gene            complement(435..1586)
                     /gene="tgt"
                     /locus_tag="PLQ30_07035"
     CDS             complement(435..1586)
                     /gene="tgt"
                     /locus_tag="PLQ30_07035"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPW02237.1"
                     /translation="MIKSIFQEKHRDPSCSARTGLISLPHGDVQTPAFMPVGTNATVK
                     AVEPRDLESMGFSLILSNTYHLFLRPGPEVISKAGGLHGFSGWQRNFLTDSGGFQIFS
                     LSQFRKILDDGVRFRSHIDGSSHILTPENVVDIQTAFNSDIQMALDICAPWGESENKA
                     YKATALTYDWAKRAKTQWLCQRERGYQGYLFGIVQGNFYRELRKMSVEQIASLDLPGI
                     AIGGLSIGEPKEAYMEYLDFTAALLPSDKPRYIMGIGTPDYIIEAVRNGIDIFDCVYP
                     TRTARNGLLFTSRGQIVIKKAIYKKDFGPIDTDCSCRVCKTYSRAYLHHLFRNNEILY
                     SMLASQHNLHFMADFASKIRQAIQEDGFESFARAFLDRYTGMEEKEEAQ"
     gene            complement(1626..2741)
                     /locus_tag="PLQ30_07040"
     CDS             complement(1626..2741)
                     /locus_tag="PLQ30_07040"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPW02238.1"
                     /translation="MAQQSSAYKQDTRFLPIILWRALLERVFIWTIGGTLFLSLVISL
                     VELFSLLWKFLAQNALFWDIIFWISLGITDHIVDALPVAFLFAIVFVLSDWHANHELE
                     AVFSAGLSLQRLLIPILSISILFCAAEFYLTDFASIPFSRSRNDLQSRILKESAGKQT
                     VPGLIVDSGKQVYTYRYFDEKNLRLYDVSVIERDAYGNLTRKISAESAKWEQGTWVFA
                     NSIIYSRQDNDWTFSKFEEYSEPVINEPPSSFTRPSMNPRFLTMRELNNHIEFLKASG
                     LPVVDAEVEKQKRLSFSLTPLIVVGLAAAFSGRFKKSVFLLSLLLSLCSATLYYVAQM
                     IASVSARSGLISPRISMWAVIIVFGALSTISYLSART"
     gene            complement(2768..4105)
                     /locus_tag="PLQ30_07045"
     CDS             complement(2768..4105)
                     /locus_tag="PLQ30_07045"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPW02239.1"
                     /translation="MKERPSFTLWRITISEIMTSFLGTFLFFFIVFLINQVLLFAEDI
                     LSRGADLLSVVKLLFYSLPTILAITIPFSILAATLMTSSRQNSDNELLASSSLGIRPL
                     WLYVPFLIIGLAISIGSFCFNEWSIPRAAQGFKNEYAELIKKSAKIELAPYSISKYGD
                     KLLVTGGSEEGSSASRLQDVLIIDQSGGYDSDTVIADDVGIEFSKDSLMAILSMNSLT
                     ELKRLNDASAGDFSVTMAKSAEIRIRIREPLMNYSSTAPSEMSLTILAKKIKEKERIL
                     DDRIKKKDFQKDTALDRLRLNYDLLAIETQQERSLKTKDSLTSLTLLQNDLISLTNKI
                     KSLENQKINDTSLQIYRLEYEKKLVLPSACFFFALLALPLGIGSKRAGRAAGFGIALL
                     LSVIYWALLFLGQTLGYRQNVNPVFSMWMPNLVMLLATISLWVVRKIKTGHFL"
     gene            complement(4131..4586)
                     /gene="dut"
                     /locus_tag="PLQ30_07050"
     CDS             complement(4131..4586)
                     /gene="dut"
                     /locus_tag="PLQ30_07050"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPW02240.1"
                     /translation="MVKPEDRAIEVLIALSEGARIPQYATDNAAGADLHAVLQKPLVL
                     EPLSRALISTGLRIALPSGYEAQIRPRSGWAYRHGVTCLNAPGTIDSDYRGEIKVILI
                     NLSDKPFVIENGDRIAQLVIAPVVRADFSISSHLDDTTRGAGGFGSTGR"
     gene            complement(4697..>5802)
                     /gene="pnp"
                     /locus_tag="PLQ30_07055"
     CDS             complement(4697..>5802)
                     /gene="pnp"
                     /locus_tag="PLQ30_07055"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPW02241.1"
                     /translation="DEQIFDDIEGDRRERFMLHYNFPPFAVGEVGRLGTGRREIGHGD
                     LARRSIEPMLPPKERFPYTIRVVAEVLESNGSSSMATVCSSSMSLMHAGVPFTKPVAG
                     IAMGLVTDDTRYAVLSDILGDEDHLGDMDFKVAGTKDGITGFQMDIKISSVSTEILRK
                     ALDQAKRGRLHILSIMEKTIAEPQSEISPYAPQVINARIDPEKIGLVIGPAGKNIKAL
                     SEKYDVQINIDEDGYLTVYGKDQKSTYSARDAILGMVEEPEVGKIYNGTVKRIMDFGA
                     FIEILPGREGLCHISKLSKEHVDKVSDLLKEGDIIKVKLMEIDHLGRLNLAAVDALDD
                     KGQIAQRDARRNDRPSDRQRDMRSSRRDSHWDR"
BASE COUNT         1748 a         1298 c         1169 g         1587 t
ORIGIN      
        1 atataatgga agtagatgtt cctgcaaacc agaaagaaat agcctatgcc ctctcactgg
       61 gcgatttgcg agaaaatgca gaatacaaag ctgcaaagga aaggcaagac gaactcaacg
      121 ccaaagtggg caaactcaaa agcgaactag atcgagccca gatcatcgat tcggatcagg
      181 tagacagcac cagtatttct tttggtacaa aaattgcgct caagaatcta atcaccggcg
      241 aagatgaatt ctttactatt ctcggaccat gggaatccga tcctgagcac caggtaattt
      301 cttatctttc tccactaggc aagcacttgc tcaatcataa gacgggcgaa aagctgaact
      361 tcacaatcaa tgagcgtaaa ttcagttatg aagtcgtaaa ggtggaaaaa gcggattttt
      421 agggtttctt gttattactg cgcttcttct ttctcttcca tgccagtata gcgatcaaga
      481 aaggctcgag caaatgattc aaagccatct tcttggattg cttgacgaat cttcgaagca
      541 aaatctgcca taaaatgcag gttgtgttga cttgcaagca ttgagtatag aatttcgttg
      601 ttcctgaata gatggtgcag ataagcacgg ctatatgtct tgcacactcg gcaactacaa
      661 tcggtatcta taggcccaaa atctttttta tatatagctt tcttgattac aatttggccc
      721 ctagaagtga ataataaacc attccgagct gttctggtag gataaacgca gtcgaatata
      781 tctataccat tccgcactgc ctctatgatg taatctggtg ttccaatgcc cataatatat
      841 cggggtttat ctgagggcaa aagagccgca gtaaagtcaa ggtattccat atatgcttct
      901 ttcggttctc cgatggaaag tccccctatc gcaatacctg gcaaatccaa ggaagcgatt
      961 tgttccacac tcatttttct aagctctctg taaaaattgc cctgaacgat accaaaaaga
     1021 taaccttgat acccacgctc tctctgacaa agccactgag ttttggctcg ctttgcccaa
     1081 tcataagtca aggctgttgc tttatatgcc ttattctcgc tttcacccca aggcgcacaa
     1141 atatccaatg ccatttgtat atcggaattg aatgcagtct gaatatcgac cacattctct
     1201 ggagttagaa tgtgagaaga accatcgata tgcgatctaa agcgcactcc atcatctaga
     1261 atctttctga attgagacag tgaaaatatc tgaaacccac cagaatccgt aaggaaattt
     1321 ctttgccagc cagagaaacc atgcaaaccg ccagccttgg atatgacttc cggacctggc
     1381 ctcaggaaaa gatgatatgt attagaaaga atcaaagaaa atcccatcga ctctagatcc
     1441 cttggttcca ctgccttgac cgtggcattt gtacctacgg gcatgaaagc aggagtctga
     1501 acatctccat gcggaagcga tattaaaccg gttctcgcag aacatgaagg atctctgtgc
     1561 ttctcttgaa agatcgattt aatcatctat agggctactc caatattatt caaaataata
     1621 catatttaag ttcttgcact caaataagag attgttgata aagcaccaaa gacaataatt
     1681 accgcccaca tggatattct cgggctgatc aagcctgatc tagcagatac ggaagctatc
     1741 atctgcgcaa catagtatag agttgcgcta caaaggctaa gaagcaagct cagtaagaaa
     1801 acgctctttt tgaaacggcc tgagaaagcg gctgcaagac caaccacaat cagaggagtc
     1861 aaggaaaaag atagcctttt ttgcttttca acctctgcat ccacaacggg aagcccggaa
     1921 gcctttaaga attcgatatg attgttgagc tctctcatgg tcagaaaacg aggattcatg
     1981 cttggtcttg tgaagctcga aggaggttca tttatcactg gttcggaata ctcttcgaat
     2041 tttgagaatg tccagtcgtt gtcttgcctg ctatatatta tcgaatttgc aaaaacccat
     2101 gttccttgct cccatttcgc actttctgcc gagatttttc ttgttaggtt tccataggca
     2161 tctctttcga ttacagaaac atcatatagg cgaagattct tttcatcgaa atatctgtaa
     2221 gtatagacct gttttcctga atcaacgatc agcccaggca ctgtttgttt tcctgcggat
     2281 tctttgagta ttctagactg cagatcattt ctcgatctag aaaatggaat ggatgcaaag
     2341 tctgtcaagt aaaactcagc agcacaaaac aatatcgaaa tacttagaat aggaatcaac
     2401 aagcgttgca gtgataatcc tgcactaaag acagcttcaa gttcatggtt tgcatgccaa
     2461 tcgctcaaaa cgaagactat tgcaaaaaga aaagctaccg gcaaagcatc gacaatatga
     2521 tcagtaattc ccaggctaat ccaaaagatg atatcccaaa aaagagcatt ttgagcaaga
     2581 aatttccata aaagagaaaa aagttctaca agagaaatta caagcgaaag aaatagagtt
     2641 cctccaatag tccagataaa tactctttcc aacagggctc tccagagaat aatcggcaaa
     2701 aatcgagtgt cttgtttata agctgatgat tgttgagcca tgttcgattc cacttccttt
     2761 ttaggcatca taaaaaatgc ccggttttta tttttctgac aacccataga gaaattgtgg
     2821 caagcaacat gacaagattc ggcatccaca tagaaaacac gggattgaca ttttggcggt
     2881 aacccagagt ctgacctagg aataacaatg cccaatagat tactgaaaga agcagtgcaa
     2941 taccaaaacc ggctgcgcgc cctgctcttt ttgatccaat tcctaatggg agtgctaaaa
     3001 gtgcaaagaa aaagcaagcg gaaggcagga ccaatttttt ctcatattca aggcgataaa
     3061 tctgaagtga tgtatcgttg attttttgat tctcaagtga ctttatcttg ttggtaagag
     3121 atatcagatc attttgtaac aaggtcagtg aggtcagtga gtcttttgtc ttcaatgatc
     3181 tctcttgctg agtctcaatg gcaagcaaat cataattcag ccttaatcta tcaagcgctg
     3241 tatccttttg aaaatccttt tttttaatcc gatcatcaag aatccgctct ttttctttta
     3301 ttttttttgc caatattgtc aaagacatct cagaaggagc cgtactacta taattcatta
     3361 agggttcgcg gatcctaatt cgaatttcgg cggactttgc catagtcaca gaaaaatctc
     3421 ccgcagaggc atcatttagt ctctttagtt ctgtaagact attcatgctt agaatagcca
     3481 tcagagaatc tttagagaat tctattccca catcatccgc tatcacggta tcagaatcat
     3541 accctccaga ctgatcgata attagtacat cttgtaatcg acttgcggaa gaaccctcct
     3601 ccgatccacc ggtgacaagc agtttatctc catatttact gattgaataa ggagctagct
     3661 caattttagc agatttctta ataagctctg catattcatt cttgaagcct tgagcggcac
     3721 ggggtataga ccattcattg aagcaaaaag atccaataga aatggctaat ccaattatca
     3781 gaaaaggaac atataaccaa agaggtctta tacccaagga agaacttgcc agaagctcgt
     3841 tatcggaatt ctgtcgcgat gatgtcatga gagtggccgc taaaatggaa aaaggaatgg
     3901 tgattgcgag tatagtaggt agagaataaa aaagtagctt gacaaccgat agaagatcag
     3961 cacccctgga taggatatcc tcggcaaaga gcaaaacttg attgataaga aatacaataa
     4021 agaagaatag aaatgtgccc aagaaagaag tcattatctc tgaaatcgtt attcgccaga
     4081 gtgtgaagga gggtcgctct ttcatctcag ctcacatctc agtcttatcc tcagcggcca
     4141 gttgatccaa aaccgccagc cccacgagta gtatcatcga gatgagagga aatcgagaaa
     4201 tcagcgcgaa caacaggagc aatgaccaac tgtgcaattc gatcaccatt ctcgatgaca
     4261 aacggtttgt cactcaggtt gatgagaata accttgattt ctccacgata atcgctatca
     4321 atagtgccag gagcgtttag gcaggtgaca ccatgcctat acgcccagcc tgatctaggc
     4381 ctgatttgag cttcatagcc cgaaggcaaa gcaattctaa gacccgtcga tatcaatgcc
     4441 cttgagagag gttcgagcac cagaggcttc tgaagaacag catggagatc cgctcctgca
     4501 gcattatctg tagcatattg aggaattcgt gctccttcgg agagcgctat tagtacttct
     4561 atcgctctat cttcgggctt caccatgtca aaatatcctt aatcactaaa tcaagatcat
     4621 ctcatgatat atccgatcca tagagatcga ctacaaagag cgaattgata ttaaattata
     4681 tcatatcgct cgaggattac ctatcccaat gcgaatcccg gcgagatgac ctcatgtctc
     4741 tctggcgatc agaaggcctg tcattccgtc tagcatctcg ctgcgctatc tgtcccttgt
     4801 catcaagcgc atccactgca gcaagattga gccttccaag atgatcgatt tccataagct
     4861 taacctttat gatatcgcct tctttaagca gatcgctaac cttgtctaca tgttcctttg
     4921 agagtttcga aatatgacat aatccctcgc gacccggaag tatttctata aaagcgccga
     4981 aatccatgat ccgctttacc gtgccgttat aaatctttcc gacttccggt tcctctacca
     5041 ttcccaagat ggcatcgcga gcactatagg tagatttctg gtctttgccg tagactgtaa
     5101 ggtacccatc ttcatcgata ttgatctgaa catcgtattt ctcagagagt gccttgatat
     5161 tcttgcctgc agggccgatc acgaggccga ttttttcggg atcgattcgc gcgttaatca
     5221 cctgaggggc atagggagat atttcgcttt gtggttcagc gatagtcttt tccataatag
     5281 agagaatgtg aagtcggccg cgtttggcct ggtctagagc cttgcgaaga atctctgtcg
     5341 aaacgcttga tatcttgata tccatttgaa agccggtgat accatctttt gtgcctgcta
     5401 ctttgaaatc catatcacca agatgatctt cgtcacccag gatatccgaa agtacagcat
     5461 agcgcgtatc atcagtaact agacccatgg ctatacctgc aacaggtttg gtaaatggca
     5521 caccagcatg cataagcgac atggatgaag aacacaccgt ggccatagag gaagaaccat
     5581 tggattcgag cacttctgcc acaacgcgaa tagtatatgg aaatctctcc tttggcggca
     5641 acatcggttc gatagatctg cgagctagat cgccatgtcc aatctcccgc cttccggttc
     5701 caagcctgcc aacttctccc acggcaaagg gaggaaaatt gtaatgaagc ataaatcgct
     5761 cgcgtcgatc tccttcgata tcatcgaata tctgctcatc ga
//
LOCUS       DAOMZL010000092         5789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_13062_length_5789_cov_16.665504, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000092 DAOMZL010000000
VERSION     DAOMZL010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13062_length_5789_cov_16.665504"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLQ30_07060"
     CDS             <1..240
                     /locus_tag="PLQ30_07060"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HPW02242.1"
                     /translation="VRSKGAILSNSSKGNPYDNALMESFYKTLKRELVNDAGFKTKDE
                     ARHALVSYIELYYNSIRKHSALGYLAPREYEELNS"
     gene            388..5481
                     /locus_tag="PLQ30_07065"
     CDS             388..5481
                     /locus_tag="PLQ30_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4011 domain-containing protein"
                     /protein_id="HPW02243.1"
                     /translation="MLAQVNLNDSDKLNAKLTTWKKKLLDTSKRNQLLNFRDYRSSTL
                     TIQEPSIKDLWERLVIDEQSLQFPLPKGNFFLDDTYSPAISDETSYENPDDIVTNKSI
                     EDLLRTLRNLRAKAKLGKEEQGINFLYLSFGFLNWKEQENSEQSFPSPLILVPVALTL
                     ESLLAPFVLSLSEDEIVTNPTLFYKLEHDFGITLPEFDPDQSPLEYFDQVQQKLGEYR
                     FGWSIQREVKLGLLSFLKINMYRDLENRSNSILRHPVIRAIGGDTSAIQDNLSLLAPI
                     NNFDHDAQEPPDTIFQVLDADSSQQDAILAAKKGISFILQGPPGTGKSQTITNIIAES
                     MAMSKSVLFVSEKMAALDVVLKRLTESNLSDFCLVLHNRKANKKDVLDQLGAVLSLSG
                     QKCNLANEAQESLDKLKKYREQLNQYDKEIHHVILPLNTTIFQVNGILASLASYKDIS
                     FSLNNIEKMSQEELSELLADLATLSSRLGDMSDDYLKNPWKGAKVEQVTNTLRYDIGA
                     FLSPVIAQISEFSEMLESAGKLLDIAFDSRLSTLDQIATFFQLASKSPKAPAQWVQSD
                     DLPELFDLVDKWFALQSQYQNIKRELEEEYHSAAALDAIGIFSANPSSSDYSLEQIKS
                     YCHEALSAQDYYRIWQSLGTARATIIDEAKSKIIQAQELVASLRLEFENDLFDLHAKE
                     LYQKFKTDYASSVRFLKRGYRTDLRKLRTCLKNPLEKMTYEKAISVLKKLRDLEELRQ
                     WRQERDIALSNCLGTLYQQESTDFALIRKAQDAYDAIIQCQSTLKNLGKIAQDESQNS
                     QYLKGHFAQFYDGFSTDWNLVRSALEWASELKKFLVVVTYTPSFIDRICTDDATIATC
                     AACADQIRQYANQITALSQWFSNLFDKDERDRIQQSPLPELAERLAACKDNLADLEKW
                     VDLNASIKKCTEDGLQDYILKIQSMHVPSKDIVNIFKKRFYYLWLDSILPRYPAVAQF
                     RRQAQEGYIKEFQTLDKLQLAIAKARVRANLINSLPPTDRFTSGADEVSILKREMMKQ
                     RKIMPIRKLFNSIPDLIMKLKPCLMMSPLSVSQFLESDCFMFDTVIFDEASQVRTEDA
                     IGAISRGSQVIITGDSKQLPPTNFFTAFLTEPEYDTSDDDEDYEDAYESVLEEAALLS
                     ERTLLWHYRSLHEDLIAYSNAKIYKNRLITFPSSFDRVPGYGVEFIFVPGGFYDQGGR
                     RGNPHEAERIADLVFEHFRTYPNRSLGVIAFGDHQQQAIEMAIRKRRLANQRYEDFFN
                     EDKFEAFFVKNLETVQGDERDTIILSVGYAKSSAGKPPTNFGPIMHSGGERRLNVAIT
                     RARYNIKLVSSIEPTDITTENFSHAGPKLLRGYMEFAQRGRTVLENEIKDSGSTQTES
                     PFEEAVYNFLAQHGGYQLAIQIGCSGYRIDIGIKHPTLSGRYVLGIECDGATYHSART
                     TRERDRLRQDVLERMGWKIYRVWSTDWIKDPLKEGNDLIRAIEKAIQSYEDDPSLQES
                     SLSTQDAEKSNYLTVSQIASEASSNSIPYGFISPKKLDQGSISYDGHYSQDKMCAMIE
                     FLVNNQYPLHIDYLCEQIASYLGCEKTIYVLRQDLICDVGILQLQKRIKKKGSFLYPA
                     AYEKIPHYVLNDRPIKYVSKDEIASAMLAIANACIGLTRESLITETIHAFGFPRAGKT
                     VKAAMNAVYEQLLQEHRLKEQDGKVSVIA"
BASE COUNT         1785 a         1240 c         1178 g         1586 t
ORIGIN      
        1 gtgagaagca agggagcaat tctgtcaaac agtagtaaag gcaatcctta cgacaatgca
       61 ctcatggaat cattctacaa aacgctgaaa cgagagctgg tgaatgatgc cggattcaag
      121 acaaaagacg aagcgcgcca tgcactggtc agctacattg agttgtacta caacagtata
      181 agaaagcatt ctgcgttagg gtatcttgct cctcgagagt atgaggagct gaattcatag
      241 atttctctta tcttcgtgtc aaaaataact ggaccctttc acttatcata atctacactt
      301 gtatcataat ctacacttgc gttcgctagc ctcttcgatg atataattta gaaaattatg
      361 cagggcaaaa atggagattc agggccaatg ctagcacaag taaatctgaa tgattctgat
      421 aaattaaatg caaaacttac cacatggaaa aagaaacttc ttgacactag caaaagaaac
      481 cagcttttaa atttcaggga ctatagaagc tctacactta ctattcaaga accttcaata
      541 aaagacctgt gggagcggct tgttatcgat gagcagtctt tgcaatttcc attgccaaaa
      601 ggcaatttct ttctcgatga tacttactct cccgcaattt cagatgaaac ctcttatgaa
      661 aatccagatg atattgtcac aaacaaatca atcgaagatc ttctaagaac gcttcgcaat
      721 cttcgggcta aagccaagct tggaaaggaa gaacagggta ttaatttcct atacctttct
      781 tttggttttc taaattggaa agagcaagaa aatagcgagc aatcgttccc ttctccactc
      841 atcttggttc ctgtcgcatt gacacttgaa tcgctccttg ctccatttgt gctctctctt
      901 tctgaggatg aaattgttac aaatcctacc cttttctata agctagagca tgattttgga
      961 ataacactcc ctgagttcga tccagaccaa agtcctctcg aatatttcga tcaagtgcag
     1021 caaaagcttg gagaatatcg ttttggctgg tcgattcaac gcgaggtaaa gctaggtctg
     1081 ttatcttttc ttaaaatcaa tatgtatcga gatttagaaa atcgcagcaa tagtatcttg
     1141 cgacatccgg tcattcgtgc aataggaggc gatacaagcg cgatacagga taacctctcc
     1201 cttcttgcgc ctatcaacaa ctttgatcac gatgcccaag agccacccga cactattttt
     1261 caagtgctcg atgcagattc gagtcagcaa gatgcaatcc ttgctgccaa gaaaggcatc
     1321 agctttatac tgcaaggacc tcctggtact ggcaaaagtc aaaccataac gaacatcatt
     1381 gccgaatcta tggcaatgag taaaagcgta ctttttgttt ctgaaaaaat ggctgcactg
     1441 gatgtggttc taaaaagatt aaccgaatct aatttatcag acttttgttt agtacttcat
     1501 aatcgaaaag caaataaaaa agatgtgctc gatcaacttg gtgcggtgct ctcgctctca
     1561 gggcaaaagt gcaatttggc taatgaagca caagagtcgc tcgataaact caaaaaatac
     1621 cgcgaacaac taaatcaata tgataaagaa atccatcatg tcatacttcc tttgaacaca
     1681 actatctttc aggtaaacgg cattctggca tcattggcct cctacaagga tatctcattc
     1741 tctttgaata atattgaaaa aatgagtcaa gaggagcttt cggaactgct tgctgacctc
     1801 gcaacattaa gttccagact tggtgatatg agcgatgact atcttaaaaa cccatggaaa
     1861 ggagcaaagg tagaacaggt gaccaatacg ctgcgctatg atattggagc gtttctctcc
     1921 ccagtaattg cacaaatctc tgagttttcc gaaatgctgg aatctgccgg caaactcctc
     1981 gatattgcgt ttgattctcg attatcaacc ttagatcaaa ttgctacctt tttccaactt
     2041 gcttctaagt ctccgaaagc ccctgctcaa tgggtacaat ctgatgacct tcctgaatta
     2101 ttcgatttag tagacaagtg gttcgcgcta cagagccaat atcagaatat caagagagaa
     2161 ctcgaagagg agtatcatag tgctgcagcc ctcgatgcga tcggaatttt ttcagcaaat
     2221 ccttcttcga gcgattattc attggaacaa ataaaatcat attgtcacga agctctgtca
     2281 gcgcaggatt attaccggat ctggcaatcg cttggaactg ccagagctac aataatcgat
     2341 gaagcaaaaa gcaaaatcat tcaagctcaa gagcttgttg catcgctaag attagaattt
     2401 gaaaatgact tgtttgatct ccatgccaaa gaattgtatc aaaaattcaa aacagattat
     2461 gcatcatcag ttcgttttct taagcgcggc tataggactg atctaagaaa actacgcaca
     2521 tgtctaaaaa accctcttga aaaaatgacc tatgagaaag caatatctgt cctaaagaaa
     2581 ttgcgcgacc ttgaggagct tcgccagtgg cgccaagagc gggatatagc gctgtcaaac
     2641 tgcctaggaa ccctctatca gcaggaatct accgactttg cccttatccg aaaagcacaa
     2701 gatgcctacg atgcaattat acaatgccaa tcaactttga aaaatttagg aaaaatagcg
     2761 caggatgagt cacaaaatag ccaatatcta aaaggccatt ttgctcaatt ttatgatggc
     2821 ttctctaccg attggaatct agtgcgcagc gcactagagt gggcatcaga gctgaagaag
     2881 ttccttgtcg tggtgaccta tacgccgtct tttatcgaca gaatttgcac cgacgatgcc
     2941 actattgcta cctgcgcagc ctgtgcggac caaattcgcc aatatgcaaa ccaaatcact
     3001 gctctaagcc agtggttttc taacctcttc gataaagacg aaagagaccg tatccaacaa
     3061 agcccgttgc ctgagctggc agaacgcctt gcggcttgca aagataacct cgcagatcta
     3121 gaaaagtggg tcgatttaaa tgcatcgatt aagaaatgca cagaagatgg tcttcaagac
     3181 tatatcttga aaattcaaag catgcacgtt cctagcaagg atatcgtcaa tatttttaaa
     3241 aaacggtttt attatttatg gctagatagt attcttcccc gctatccagc agttgcgcaa
     3301 ttccggcggc aagcacagga aggatatatt aaggaatttc aaactctcga caagttacaa
     3361 ttggctattg caaaagcacg agttcgtgcc aatcttataa attcgcttcc tcccacggac
     3421 cgctttacct ctggagctga tgaagtaagc atcttaaagc gcgaaatgat gaagcaacgc
     3481 aaaataatgc cgattcgaaa gctattcaat agtattcctg atttgattat gaaacttaag
     3541 ccctgcctca tgatgtcgcc tttatcggta agccagtttt tggaatctga ttgctttatg
     3601 tttgacacag tcattttcga tgaagcttca caagttcgta cagaagatgc tatcggcgcg
     3661 atttcgcgtg gttcccaagt aatcatcact ggtgatagca aacagctccc gccaacaaat
     3721 ttctttacgg cttttctcac tgagcctgag tatgacactt ctgatgatga cgaggattat
     3781 gaagatgcct acgagtctgt actggaagaa gcagcattgc tttcagagcg cactctctta
     3841 tggcattacc gaagccttca tgaagacctc attgcatatt ccaatgcaaa gatctataaa
     3901 aaccgcctca ttacctttcc ttctagcttt gacagagttc ctggttacgg cgtcgaattc
     3961 atctttgttc cgggcggctt ctacgatcag ggtgggagga gaggaaatcc gcacgaagct
     4021 gagcgtatcg ccgatcttgt gtttgagcat tttcgtacct atccgaaccg ttcccttgga
     4081 gtcattgcat tcggagatca tcaacagcag gcaattgaaa tggctatcag aaaaaggcgc
     4141 ctcgcaaatc agcgctatga agatttcttc aatgaagaca agttcgaagc cttttttgtt
     4201 aaaaatttgg aaactgtcca aggcgatgag cgtgatacta ttattctcag cgtgggctat
     4261 gcaaaaagct ctgcggggaa accgccgaca aattttggcc ccattatgca ttcgggcggc
     4321 gagcggcgtc tcaatgttgc cattactcgt gcaagatata atattaagct agtctcttct
     4381 attgagccga cagacataac tactgaaaac ttctctcatg cagggccaaa gctcctacgt
     4441 ggatatatgg agtttgcgca gcgcggtcga acagtcttag aaaatgaaat aaaagactcc
     4501 ggtagcacac agaccgaatc accttttgaa gaagctgtgt ataatttttt agcgcagcat
     4561 ggcggctatc aattggcaat ccaaattggc tgttctggct accgcatcga tataggcatc
     4621 aagcacccta cactttctgg cagatatgta cttggcatcg agtgcgatgg ggctacctat
     4681 cattcagctc gaaccacacg agaaagagat agactccgac aagatgttct tgaacgaatg
     4741 ggctggaaga tttatcgcgt ttggtctacc gactggatca aggatcctct caaagagggg
     4801 aacgatttaa ttcgcgctat tgaaaaggca atccaaagct atgaagacga cccttccttg
     4861 caagaatctt ccttatcaac acaggatgca gaaaagagca actatttaac agtttcacaa
     4921 atcgcttcag aagcatcctc taatagtatt ccttatggct ttatttctcc aaaaaaatta
     4981 gatcaaggca gcatatccta cgatggtcac tatagccaag ataaaatgtg cgcaatgatt
     5041 gaattcttgg ttaataatca ataccctttg catatcgatt atttatgcga acaaatagca
     5101 tcctatttag gatgcgaaaa aacaatttat gtgctccgac aggaccttat ctgcgatgtc
     5161 ggcatattgc agctacaaaa gcgtattaag aagaaaggca gcttcctcta tcctgccgcg
     5221 tatgaaaaaa ttcctcatta tgtgctgaat gaccgtccaa tcaaatatgt gagcaaagat
     5281 gaaattgcct ctgcaatgct tgctattgcg aacgcatgca tcgggcttac acgagaatca
     5341 ttgattactg aaactatcca tgcctttggc tttcctcgcg caggaaaaac tgtcaaggct
     5401 gctatgaatg cagtatatga gcagctactt caagagcatc ggctaaagga acaagatggt
     5461 aaggtatcgg ttattgctta atgttaacca tgtaagagag ccttgtcatg atttatctct
     5521 aatttgagag attgaattgc gtaaagtgat gtttgcaatt tggttattaa aaaagacatt
     5581 ggaagcttta aaatgttttt ttatacaaca agcacaatga atgacactag tgaaggaata
     5641 gaaagtggca gattttttcg tcctatttta ggaggataga tatgaaaagc gacgaaataa
     5701 agcaaatcat aactactaaa tattcgaaaa ttgcaaaaag cggttgcgga tgcggcaata
     5761 cttcatcatg ttgcggtagt tcgctttcg
//
LOCUS       DAOMZL010000093         5726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_13276_length_5726_cov_13.826486, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000093 DAOMZL010000000
VERSION     DAOMZL010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13276_length_5726_cov_13.826486"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PLQ30_07070"
     CDS             <1..897
                     /locus_tag="PLQ30_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HPW02244.1"
                     /translation="IGEDYSDTPWIALSQGYHRMAPEDRYQLLRNRAVKTMIDVGIPV
                     KYHHHEVVAAQLEIELDFISMLKAGDAVSLAKWILRSEADELGLKVTFMPKPMYKVAG
                     SGMHVHQFIEKLGASIFPGEELYGLSREGLSYTAGLLSHALTGSLLAFSNPSTNSYRR
                     LVPGYEAPVSATFAHGSRAAAVRIPGYLGKGEARIEFRTGDATANVYYFLSAMLLAGL
                     DGINKRLDPVALGFARETTSARHVFPTGLFDVLAGLKKDHGYLSAAFSEELVETWIAH
                     KEKEASYVYNAPVPQEYELYFD"
     gene            986..2629
                     /locus_tag="PLQ30_07075"
     CDS             986..2629
                     /locus_tag="PLQ30_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPW02245.1"
                     /translation="MSQKVLLSGDEAIAHAAMDSGITAAFAYPGTPATEIMEYLQECI
                     SNTDNDESIGARDFRPVAQWCTNEKTAYESALGVSFAGRRTMVSMKHVGLNVAMDPFV
                     NSALVRINGGLVVIVADDPGMHSSQNEQDSRLLADFARIPCFEPSDQQEAYDMVRAAF
                     DYSEAHEVPVMVRIVTRLAHARGLVQTAPSVPICNRGKTTDRFSWTLLPASARKQWLA
                     LLAKQPAFKVDGEKASRLELRSKELGVVTCGLALAYYKENEGDWTVAHGGEPPSHLHL
                     GRYPIEVSEGESKVRALAAHVSQILVIEEGYPYIEREMRGIFGAPIPILGKMTGELPL
                     AGELSSDSVRIALGLPLVSSSLHSEIELPSRPPQFCQGCPHADSYTALKKALEGEAEF
                     FVFSDIGCYALGAQQPWNIIESCVDMGASVGMARGAGCVGNKRSIAVIGDSTFYHSGL
                     TNLLDAVQHHSPMTLMILDNGTTGMTGAQPTISPSQQLPKLLEGFGVEREHIRVLEAH
                     RRALDANVEIIREELSYDGVSVIIMFRECIEWLKKARRS"
     gene            2602..3204
                     /locus_tag="PLQ30_07080"
     CDS             2602..3204
                     /locus_tag="PLQ30_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPW02246.1"
                     /translation="MAKESEEIMTCDIILCSVGGQGGLSASIIIARAAMAAGFQVKQS
                     EIHGMSQRGGEVLAHLRIADKEIYSPTISKGKADIMFALEPLEALRHLSWISPSNGII
                     VSATAPVLNIPDYPDLDTVLAEIRKIARVRLIDAEAIARKSGNVRTANLVLIGAAMDL
                     LPISPDAIEKEIIELFSRKGESVVQANLKAFEAGRALYHG"
     gene            3197..5641
                     /locus_tag="PLQ30_07085"
     CDS             3197..5641
                     /locus_tag="PLQ30_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPW02247.1"
                     /translation="MVERQKTQKTKDQATTQYAQGGCRLSQSAKELRDKIKRIISQAQ
                     SEHREALTELEGMSILAAMGVQTPRRWIVQSANEFLEKIGAEGAIDAPLQSPFPGSKA
                     VIKVISSKVLHKTEVQGIEIVDNTATAIADSLDRMEARFKNVPRDGFTVNEFIAFEPK
                     LGHEMIFGYRFTPDFGPIVTFGPGGIYTEYLASKFKPGMANLFLSPRINDMAVLESLL
                     RNNVIYGLLCAGLRNTRPELEEKSLIEAIQRFIDAADALSAAGISEFEVNPMVLSKTG
                     ELVALDCLATLKEFAENAPANGSAQSSSQNLIQSLVRDSEGFPINFAQHIRPVNQIKH
                     ILEPSSVAIIGVSEKTINNGRIILRNLLENNFDKSRIYIIKPGAQWIDGCQCVPDVQS
                     LPEKIDLFVVVIPALGIPSTLADIARYDKAWSVLLIPGGLEEKQGSESIVADMNRALA
                     EARAEGKGPLINGGNCLGIRSVPGKYNTLFIPEYKLPMPKGKIEPLALISQSGAFAIT
                     RISKHPNINAKYAITIGNQMDLTVGDYLDYLAQDNELHVFAVYSEGFKPLDGQKALEA
                     TKEITMSGRNVILYRGGRTRAGAGAAASHTASIAGDYPVTWQLFSQAGAVVCDSLDEF
                     DDAITLFTLLDGKRAKGRRLAAVSNGGYECVAIADNLGEMVLSHFSERTKTELEVIYK
                     DAHISEIVDIHNPLDLTPMADDDAYERAVRTVLMDPETDLCIVGIIPLTAKLNTLASG
                     PLSHGEDILRPNSLPERLGRLSSEMDKPFIAIVDSGPLYDPFSAELEKRGIPTFRAAD
                     RALRMLERWRKAHQRI"
BASE COUNT         1457 a         1355 c         1496 g         1418 t
ORIGIN      
        1 attggcgagg actatagcga tacgccatgg attgcgcttt cccaagggta tcatcgtatg
       61 gctcctgagg ataggtatca gcttttgcga aatcgtgcgg taaagacaat gatcgatgtc
      121 ggcattcctg tcaaatacca tcaccacgaa gtggttgctg cgcaactcga gatcgagctg
      181 gattttattt cgatgctgaa ggcaggagat gccgtatcct tggctaaatg gattctacgc
      241 agcgaggcgg atgagctggg gctaaaggtc acttttatgc ccaagcccat gtataaggtt
      301 gcgggcagcg gcatgcatgt gcatcaattc atagaaaagc ttggagcttc aattttccca
      361 ggtgaagagc tgtatggact ttccagggaa gggctgtcat acacggcagg actcttgagc
      421 catgctctca cgggctctct cctggctttt tccaatcctt ccactaattc atatcgaagg
      481 ctcgtaccag gttatgaggc gccggttagc gcaacctttg cccatgggtc gagggcagca
      541 gcggttcgaa ttcctggcta tctaggaaag ggagaagctc gcatagagtt tcgcacaggg
      601 gatgccacgg caaatgtata ttatttttta tccgcaatgc ttctcgccgg tctcgatggg
      661 ataaataaga gactcgatcc cgtagctcta ggctttgcgc gcgaaacaac ttcggctcgg
      721 catgtcttcc caactgggct ttttgatgtg ttagccgggc tcaagaagga tcatggttat
      781 ctttcggccg cgttttccga ggagcttgtc gaaacctgga tagcgcacaa ggaaaaagaa
      841 gcctcatatg tgtataacgc gcctgttcca caggagtatg agctatactt tgattagaca
      901 gggagattgt tggcgcaggt ttgttcctgc gctttttttt attttcttgg attgttgtct
      961 gactggatga ataaggagtt cggaaatgtc acaaaaggtc cttctttctg gagatgaagc
     1021 gatagcacat gccgcgatgg attcgggaat tacagcggcc tttgcgtatc caggaacacc
     1081 ggcaactgag atcatggaat atttgcagga atgcataagc aacacagaca atgatgaaag
     1141 cattggtgcg agagacttcc ggcctgtagc ccaatggtgc actaatgaga agactgcata
     1201 cgagtccgca ctaggcgtat ccttcgcggg taggcgcacc atggtttcta tgaagcatgt
     1261 tggactcaat gttgcgatgg atccttttgt gaattctgcg ctcgttagaa taaatggggg
     1321 gctcgttgtc atagtggccg acgatccagg tatgcattcc agccaaaatg aacaagactc
     1381 gagacttttg gcagattttg ctcgcattcc ttgttttgaa ccatcggatc agcaggaagc
     1441 ctacgatatg gtgcgagcag cattcgacta ttcggaggcg catgaggtgc cggtgatggt
     1501 tcgcatagtg acaaggcttg cgcatgcccg aggtctggta cagacagcac cttcagtgcc
     1561 tatctgcaac cgtggcaaga ccaccgatcg tttttcttgg accctattgc ctgcttcggc
     1621 gcgaaagcag tggttggcct tgctagcaaa acaacccgcc ttcaaggtag atggagaaaa
     1681 ggcgagccgc cttgagctgc gatcgaagga acttggtgtg gtgacctgtg ggctggctct
     1741 ggcgtattat aaggaaaatg agggagactg gaccgtggct catggcggcg agccgccttc
     1801 acacctacac ttaggccgat atccaataga ggtttcggaa ggcgaatcaa aggttagagc
     1861 gcttgctgcg catgtgtcgc aaattctggt aatcgaagag ggctaccctt acattgagcg
     1921 cgaaatgcga ggcatattcg gagcacccat accgattttg gggaaaatga cgggagaatt
     1981 gcctctagcg ggcgagttga gctcagattc ggtgcgaata gctcttggac ttcctttagt
     2041 ctcctccagc ctgcactcag agatcgagct tcctagccgt ccaccccaat tctgtcaggg
     2101 ttgtccacat gccgattcct atacagcgct caagaaggct ctcgaaggag aggcagaatt
     2161 ctttgtcttt tcggatatcg gttgctatgc tcttggtgca cagcaacctt ggaatatcat
     2221 agagtcctgc gtagacatgg gcgcttcggt gggtatggcg cgcggtgcag gctgtgttgg
     2281 aaacaagcgc tccatagccg taataggcga ttcgactttt tatcattcag gccttacaaa
     2341 cttgctcgat gcggtgcagc accatagtcc gatgaccttg atgatcttgg acaatggcac
     2401 tactggcatg accggagccc agcccacaat cagtccaagc caacaactcc caaagcttct
     2461 cgaaggtttt ggtgtcgaaa gggagcatat ccgtgttctc gaggcacatc gtcgtgcatt
     2521 ggatgctaat gttgagatca ttcgcgaaga gctaagttat gatggtgtgt ctgtaatcat
     2581 aatgttccga gaatgcatcg aatggctaaa gaaagcgagg agatcatgac ctgcgatata
     2641 attctctgca gcgtcggagg ccagggcggg ctttctgcgt cgatcataat agcccgcgcc
     2701 gcaatggccg ctggatttca ggttaagcaa tccgaaatac atgggatgtc tcagcgcgga
     2761 ggtgaagttt tggctcacct gcgcattgca gacaaggaga tctatagtcc cacgatttcc
     2821 aagggcaaag cggatattat gtttgctcta gagcccctgg aagcgctccg tcatctttcg
     2881 tggatttccc cctctaatgg tattattgtc tcagccactg cacccgttct caacattcct
     2941 gattaccccg atctggatac cgtgttggca gaaatccgca aaatcgctcg agtacggctt
     3001 atcgatgctg aagccatagc gcggaaatcg ggaaatgttc gaactgcaaa cctcgtactg
     3061 atcggtgccg ctatggatct tttgcccatt tctcctgatg ccatcgagaa ggagataatc
     3121 gagctctttt caagaaaagg cgaatcggtt gtacaagcca atctaaaggc ttttgaagca
     3181 gggagagcgc tgtatcatgg ttgaaagaca aaagacccaa aagaccaaag atcaggcgac
     3241 aactcaatat gctcaaggcg gctgtcgcct gagtcaatcg gcaaaggagc ttcgcgacaa
     3301 gataaagcgc ataatatccc aagcccaaag cgagcatagg gaagcgctta cggagctcga
     3361 aggaatgtcc attctggcgg caatgggtgt acagacgccc cggcgttgga ttgtgcagtc
     3421 ggcaaatgag tttctggaga aaataggagc tgaaggagca atagatgctc ctctgcaaag
     3481 ccctttcccc ggctccaaag ctgtaatcaa ggtcattagt tctaaggttc ttcataagac
     3541 cgaagtacaa ggcatcgaga ttgtcgataa cacggctacc gcaattgccg attcgctcga
     3601 tagaatggaa gctcgattca agaatgtgcc tcgcgatgga tttactgtca atgagttcat
     3661 agctttcgaa ccgaagcttg gtcatgagat gatttttggt tatcgtttta cgcctgattt
     3721 tggccctatt gtgactttcg gccctggtgg tatttatacc gagtatctcg catcgaaatt
     3781 caagcccggt atggcaaacc tttttctctc tccaagaatc aatgacatgg cagtgctcga
     3841 atcgcttcta agaaacaatg taatatatgg gctcttatgt gctggcctaa gaaatacaag
     3901 gccagagttg gaagaaaaga gtctcatcga agcaatccag cgctttatcg atgccgcaga
     3961 tgccctttcg gccgcaggca taagcgaatt cgaggtcaat ccaatggtgc tttcaaaaac
     4021 cggggagctt gtagcactgg actgcctggc aacgctaaaa gagtttgcag agaacgcgcc
     4081 agcaaatggc tctgcgcaga gttcttcaca gaaccttatt cagagccttg tgcgcgattc
     4141 cgagggtttt cctatcaatt tcgctcagca tattcggcca gtcaaccaga taaagcacat
     4201 tctcgagcct tctagcgtgg ctatcattgg cgtatctgaa aagacgatca acaatggtcg
     4261 tattattctc cgcaatctcc ttgaaaataa cttcgataaa tctaggatct atatcataaa
     4321 gccaggtgct caatggatcg atggttgcca atgtgttccg gatgttcaat ctctgcctga
     4381 gaaaatagat ctctttgttg tggttattcc agcgcttggt attcccagca cgcttgccga
     4441 cattgcgcga tacgacaaag catggtcggt tctcctaatt cccggagggc tggaagaaaa
     4501 gcaaggctca gaatccatag ttgcggatat gaatcgtgct ttggcggaag cacgcgccga
     4561 gggaaaggga cccctgataa atggtggcaa ctgccttggt atacgctcgg ttcccggtaa
     4621 atacaacacc ctcttcatac ctgaatacaa gctacccatg cccaagggca agatcgagcc
     4681 acttgcgctg atctcacagt caggcgcatt tgccataacg cgcatctcta agcatcccaa
     4741 catcaatgca aaatatgcaa taaccattgg caaccagatg gacctaacag tcggagatta
     4801 tctggattat ctggctcaag ataacgaact ccatgtattt gcggtctatt ctgaaggttt
     4861 taagccactg gatggacaga aggcactcga agcgaccaaa gagattacca tgtccggtcg
     4921 caatgttatt ctataccgag gaggcaggac tcgagcagga gcgggtgcgg cagcgagtca
     4981 tactgcaagc attgcgggag actatcctgt tacttggcaa ctcttttctc aagccggcgc
     5041 ggttgtttgc gacagtctgg acgaatttga cgacgcgatc acattattta ccctgctcga
     5101 tggtaaaaga gcaaaaggca gaaggctcgc agccgtctcc aacggcggct atgagtgcgt
     5161 tgcgattgca gacaatctgg gggagatggt tttatctcat tttagtgaaa ggacaaaaac
     5221 cgagcttgaa gtgatttata aggatgcgca tatatcggaa atagtagata tccacaaccc
     5281 actcgatctc accccaatgg cggacgacga cgcctatgaa agggcggtta gaacagtact
     5341 tatggacccc gaaaccgatc tctgcattgt gggaataatc ccccttaccg ctaagttgaa
     5401 tacgcttgca agcggcccat tatctcatgg cgaagatatc ctcagaccta attcgcttcc
     5461 cgaacggctc ggcagattat cgagcgaaat ggataagcct tttattgcca ttgtcgattc
     5521 tggtccgctc tacgatcctt tttccgcgga gctcgaaaaa cgagggattc caacttttcg
     5581 cgcagcagac cgagccttgc gaatgctgga gcgctggcga aaagcccatc aaagaatata
     5641 gagcggcaag gtgcaagaga tatcggctat ttacatacgc gcaaacacat cttcgagcgt
     5701 tccggagaaa ggcccgggct tttcca
//
LOCUS       DAOMZL010000094         5548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_13789_length_5548_cov_11.833060, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000094 DAOMZL010000000
VERSION     DAOMZL010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13789_length_5548_cov_11.833060"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..456)
                     /locus_tag="PLQ30_07090"
     CDS             complement(142..456)
                     /locus_tag="PLQ30_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02248.1"
                     /translation="MPQRIQMIHGVPYVYEDSATWDKEKKNAKHARHYIGKMVDGVFV
                     PNKTYELECALKESKEKKPGPQENTQSIRQFCGATYLFDRIGEKLGITEDLEKCFPDI
                     YK"
     gene            complement(854..1525)
                     /locus_tag="PLQ30_07095"
     CDS             complement(854..1525)
                     /locus_tag="PLQ30_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02249.1"
                     /translation="MQYNELLEEYKIASEKEMNLERNIWESAGVVGIGSLASIVTSLR
                     GFDVLSVSLLGAFSVSLVWIWWFMAKRWWDIQHVILLRKQHIENATNMKLETYINFCD
                     REHKDREYKESNIEGLTTRNLDELKKIEDKGNGKFGKRGVQKSLRWFPRVVTAIWLAL
                     ATKGIITECYRANSCAVGSTVVPIGIGVFCILVFVFYDWRDSKKNKKENGDSNKTENP
                     ETPSS"
     gene            2036..2203
                     /locus_tag="PLQ30_07100"
     CDS             2036..2203
                     /locus_tag="PLQ30_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02250.1"
                     /translation="MDSYRRDVEQYVEDANNYIRAASNDIDSINDAMRDARNDANQVV
                     SEYNSYVTYGF"
     gene            2353..2952
                     /locus_tag="PLQ30_07105"
     CDS             2353..2952
                     /locus_tag="PLQ30_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HPW02251.1"
                     /translation="MNQKKVEAIRSFVASQGGYVRAKTLREKGIHPSQISALMESGVL
                     VRLKRGLYTIAQDIKRSELVDVQKAIPGGIFCLGTALSIHGIGTWEPPELHLAIRRDY
                     RVAVPPYPPIRFFSFSRARFELGIEEKVFESGIIRVYDAEKTICDVIRFRNTLGMDVA
                     MEALREYVKRRDRNVSRLLEYAKLLRMEGSIRTYLEPLL"
     gene            2949..3821
                     /locus_tag="PLQ30_07110"
     CDS             2949..3821
                     /locus_tag="PLQ30_07110"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPW02252.1"
                     /translation="MINKEASIRAHLKTEAARRGISFQYATLLYMHEGLLRRLAASPY
                     ADRFVLKGGLLLQCLSAYFARTTKDIDLLGMGIPNDSDRLEAAFRIICQSNQDDALYF
                     DSESLGIEEINEAGNYHGARVRVACNLGNIHNVVQVDIAFDDAVEAPLNEIEYPTLLN
                     SLHFNLLTYPLASVIAEKFETMIALGDINSRMKDFWDVAYLLGNYNFTDEELLVALKA
                     TFQRRNTPMPEDPSVFASDFENSKVLVNRWQAFLRRTLVPHQDWNEVLAVIRTRLSPL
                     YEEIRSTRTREIRE"
     gene            3927..4151
                     /locus_tag="PLQ30_07115"
     CDS             3927..4151
                     /locus_tag="PLQ30_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02253.1"
                     /translation="MSYLAAIADYNADFNTTFDTSAEKFENYYKDVSLRMKRREVDLL
                     VVVNMFLTGFDAIRPTTCGSPRTLSSMDLS"
     gene            4887..5054
                     /locus_tag="PLQ30_07120"
     CDS             4887..5054
                     /locus_tag="PLQ30_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02254.1"
                     /translation="MAENEKKPDTRTKQERDDAFYEQIRLNLDSILARLPPIRLKLFI
                     SPTKSPPEKRK"
BASE COUNT         1568 a         1230 c         1170 g         1580 t
ORIGIN      
        1 agtgatcatc ccaaagagtt cacttgagcg ctgagaagga atattcttac tataagggtg
       61 gtggtgtgtt tttgcccact taggaaagcg tcgtaaagga tttttatcct cgagaatgag
      121 atagtaggca atagaaagca gttacttgta gatatcaggg aaacactttt caaggtcctc
      181 agtgatgcca agcttttctc ctattctgtc gaagaggtac gttgctccac agaactgcct
      241 tatactctgg gtgttctctt gtggtcctgg ctttttctct ttcgattctt ttagtgcaca
      301 ttctaattca taggttttat tagggacaaa gactccatcg accatttttc caatatagtg
      361 acgagcatgt tttgcattct tcttttcttt atcccaggtt gcagaatctt catagacata
      421 gggtacacca tggatcatct gaattctctg tggcataaca atctcccctc attgatatat
      481 tgtttaccaa tgataagaag atgtcaaata ttattttctt ataaaactat aagttaaaat
      541 attccttggt ataatttctc gggaatcgag gaattaaggg caaaacagta gaaaaaaacc
      601 ctgcgacgca gaatgcttga gatctaccgt ccaccttcac ctcagctcct tttcagcaga
      661 agacagcttc ctacctaatg ccaaaagtgt gtaaaaccct tgtactcttc ttgtgataat
      721 gggttacatg cgaagagtcc ttttcgacta cacaaattga aacctgctgg cgttttctag
      781 gatacttgat gagaagacag acatagttcc agccaaaata tctgcctttt tgactgtgtc
      841 cagcgagata tacttaacta gatggtgttt caggattttc tgttttatta gaatcaccat
      901 tttccttctt atttttcttt gaatccctcc aatcataaaa tacaaaaact aagatgcaga
      961 atacgcctat ccctataggt accacagtag agccaacagc acaagaattt gccctataac
     1021 attcagtaat gatcccttta gtcgccaagg ctagccatat cgctgtaacg actctaggga
     1081 accatctcag actcttttgt acacctcttt ttccgaattt tccattgcct ttatcttcta
     1141 tctttttcag ctcatcaagg tttcttgttg tcagtccttc aatattcgat tctttatact
     1201 ctctgtcctt atgctctctg tcacagaagt tgatgtaagt ctccagcttc atattagtgg
     1261 cattctctat gtgctgcttt ctcaataaaa tgacatgttg aatgtcccac caccgttttg
     1321 ccatgaacca ccatatccag acaagtgaca cagaaaaagc gccaagcaag gaaaccgaga
     1381 gaacatcgaa cccccttaag ctggttacaa ttgaagcaag actaccaatt cctacgacac
     1441 ccgcggattc ccagatattt ctttccaaat tcatctcttt ttcggacgca attttgtatt
     1501 cttcaaggag ctcattgtat tgcatgctcg tgtcgacctt ttcttcttgc atacaccctc
     1561 cttcagaaat catccagcta tttctctggt atccaaagat gcatagtctg gcagcacgtg
     1621 aacctggtac tctagatttt aactttggtt ccacgagatc gcaagaatag ctaccttaga
     1681 tacctgtaat ccttaagtcc tttttctttt ttattcttca cgctcaagta tctcttctgc
     1741 ttgcggcaag gtcggcgcaa atttcccggt tttggtctaa tctaaccctc gtcgtaaagg
     1801 ccacgatgag cggaatgtat gggcctattt ggtggcagta atacgcgttt cgttctcgca
     1861 acaaaaatgc agcttagata cggcagtcta actgacttag gggcttgcct ctagtgccac
     1921 taattctacc aaccaaacat ggcttgccct tgtgcgacaa aatcagtaat tgagctccaa
     1981 aacagtggta ttttgcttta aattaggcgt tttctagcgt tgttgtagaa tttatttgga
     2041 ttcttataga cgcgatgtcg aacagtatgt tgaggatgca aacaattata tacgcgctgc
     2101 ttcgaacgat atcgattcga tcaatgatgc aatgcgagat gccaggaacg acgccaacca
     2161 agttgtttcg gaatacaaca gctacgtgac ctatggtttc tagaacttgg aggctagctc
     2221 tctcttcacc gactattcat ccatgttcca aaagcagaca gaccaggcca gttcacgcgt
     2281 gggaaataga ggaaatagta tcaaacacac cacacttgcg gaaaactgtg gtgtgtttta
     2341 tcatatcctt ctatgaacca gaagaaggta gaagcgatcc gttcttttgt tgcttcgcaa
     2401 ggtggctacg taagagccaa aaccctccga gaaaagggaa tccacccaag ccagatcagt
     2461 gccctcatgg aatccggcgt actggttcgt cttaaacgtg gactctacac gatagcccag
     2521 gatatcaagc ggtcggaact cgtcgatgtg caaaaagcta tcccgggcgg gattttttgt
     2581 ctgggtaccg cactttcgat acatgggatt ggtacttggg agccaccgga gcttcacctc
     2641 gccattcgac gggactaccg ggtggcagtg ccgccctatc ctcccattcg gtttttctca
     2701 ttttctcgcg cccgtttcga gctcggaatt gaagaaaaag ttttcgagtc aggaatcatt
     2761 cgcgtctatg atgcggaaaa gacaatctgt gatgttatta ggtttaggaa tacattgggg
     2821 atggatgtcg caatggaagc tctccgggag tatgtcaaaa gacgagatcg aaatgtttct
     2881 cgccttcttg aatacgcaaa actcctacga atggaaggtt cgattcgcac ctatttggag
     2941 ccgctcctgt gatcaacaag gaagcttcga tcagggcgca tctgaaaacg gaggcagccc
     3001 gcagaggaat tagctttcaa tatgcaacat tactctacat gcatgagggc ctgctcagaa
     3061 gactggctgc atctccttat gcggatcgct ttgtcttaaa aggcggcctt ctcctccagt
     3121 gtttgtcagc gtacttcgct cgtacgacaa aagatatcga ccttcttggc atgggaatcc
     3181 ccaacgactc ggacagactt gaagcagcct tccgcattat ctgtcagtcg aaccaagatg
     3241 atgcgcttta tttcgattcc gagtcgcttg gaatcgaaga gatcaatgaa gcaggtaact
     3301 atcatggagc tcgagtccgt gtcgcctgca atcttggcaa tattcacaat gtcgttcagg
     3361 tcgatatcgc gtttgatgat gcggtagaag ctcccctcaa tgagatcgag tatccgacct
     3421 tgctgaattc tcttcatttc aacctgctca cctatcctct tgcatctgtg atagcggaga
     3481 aatttgaaac catgatcgct cttggcgata tcaacagtcg gatgaaggat ttttgggatg
     3541 ttgcctacct gctgggcaac tacaatttca ccgatgaaga gttgctggta gcattgaaag
     3601 cgactttcca aaggcgaaac acacctatgc cagaagaccc gagtgtgttt gcctcggatt
     3661 ttgagaactc gaaggttctt gtaaatcggt ggcaagcctt cttaagacgg acactggttc
     3721 cacatcaaga ttggaacgaa gtccttgctg ttattcggac aagactaagt ccgctctacg
     3781 aagaaattcg aagcacaagg acgagagaaa taagggaata gacgtctcca agcttctctt
     3841 acacgcgaat cccatgaatc cagtctttcc aaatagaaag caccaactcc tcatcattgc
     3901 ctgaaaacaa ggattccaag gatgatttgt cctatttggc agccattgcc gactacaacg
     3961 ccgacttcaa tactaccttc gacacttcag ctgagaagtt cgagaactac tacaaggatg
     4021 tgtctctccg catgaaaaga cgcgaggtcg acttgcttgt tgtcgttaat atgtttctga
     4081 cgggctttga tgctatacgc ccaacaacct gtggttcgcc aagaacctta agcagtatgg
     4141 acttatccta acgttttttg tgcccattat cggggaacag aacgagaagg agtttatacg
     4201 gacctttggg gcattcctca aggtccgcaa tatcctcaca gcctttgaag cgtttgaggg
     4261 caagggaatc ctctctgagc gggaactcca agactggaag ggtccacttt attcaatttt
     4321 tcgtcaataa tgatgaaata aatcatttca gcgactttgc aagatttgtt gaagcatata
     4381 acaggcaagc cataaaagaa actcccctat tattggcgac aaaatatact atgcaaatct
     4441 acactatgga ttcattcgcc ttgaaattat cataccgcca aaaaattctg tcagcatccc
     4501 ttttgtactt caaatttgaa cttgtgatat gatttgtaaa ttgaggcaat tgaaatgata
     4561 gcttcatatc ctactcggtt ctatcgaaat cgtcaccgag ctttccaatt gcgaaatatc
     4621 cctcattcaa agaaactata tgctcttgct attcatagac aaaaaatcac aatggaatca
     4681 attgaagaat gttactgtga tggtgctaat ccttttgtct atctaccatg ctcaaggtta
     4741 gaagcaattt gcttagttaa gaggatgata agagcagagc atgatgagca gcgccttggg
     4801 gccttacttc gtcttctgga cgatattaaa aacctagaca gatgatagac taaaatcaga
     4861 gtagcaaaga aaggaggaga aagccgatgg cagaaaatga gaaaaaaccc gatactcgaa
     4921 caaaacagga aagggatgat gctttttacg aacagataag gctcaatctg gattctatat
     4981 tggcacgttt gccaccgatt cgcttaaagc tctttatttc gccaacaaaa tctccaccag
     5041 aaaagcggaa gtgatggaga tatgccattg ctttttacct tgaaaatagg atgataacct
     5101 ctattgtgga cgagaatcgg gatatgacca caaatgttgt ggacgacttg caagcaaaaa
     5161 gtatgagcat tagaggatat ggactatgga ctactacgag atcatgcgac attgtagcgc
     5221 gataacatgg aaatatgacc atggagagct ttcggagctt tcaaaactgg ataggagaca
     5281 agcggtatag cttttagcaa aagatttttg gcaatataat gtcgttctgg gcaatgacag
     5341 gcgaaccaaa agcgaaggag tgcaaggacg ccgtgactta agacattgag gaaaatcatg
     5401 cgctgcatgt agaagatatg taggatagaa aatgttctca atgaggggaa gtattgatat
     5461 taaaggctat tctatgataa aggcgtgggg tttaattccc cccacctcca tcttgaatcc
     5521 tctattcata cacgctagat tgttcaaa
//
LOCUS       DAOMZL010000095         5459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_14060_length_5459_cov_15.553109, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000095 DAOMZL010000000
VERSION     DAOMZL010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14060_length_5459_cov_15.553109"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="PLQ30_07125"
     CDS             <1..1052
                     /locus_tag="PLQ30_07125"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HPW02255.1"
                     /translation="ILFFCISFLEDTGYIARAAFLTDHFLHAIGLHGQSFMPLMLGFG
                     CSVPAIMATRTLKSPKERIATILAIPFISCGGKLPIYVLLAGTFFPNHESTVVMLVYA
                     TGVVLSILSTYFLRRTVLKGQSMPFVMELPPYRMPTLKGVLWHVWEKTWSYVRKAGTI
                     ILAVSVLMWVITSYPKAKGIDDYALQVRQEIATAHPDFDESLIAANVEILLRQYQLER
                     SIAGNIGKIIEPVIAPIGFNWKIGIALLPGLTAKELVVSTLGVLYGTTVNDETEANSS
                     LRQALRNSPDWSPRIALALLIFILVMPPCFSSLAVIRTEAGDKWLAFQVVYSLVLAWV
                     LSFLASMAGHLLGLG"
     gene            complement(1059..2408)
                     /locus_tag="PLQ30_07130"
     CDS             complement(1059..2408)
                     /locus_tag="PLQ30_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02256.1"
                     /translation="MRSSRYALFFALLFIGAILFGAAGQSASAQSASTQAQPVVSQKQ
                     DIAVFALGYYGWLIPSQALGTIDLKIQEVFAKIGRFNVFGTTQRLSSNGVQEFIDTLR
                     RSKEANFVLPEQYQFGEAFLTKAEFERLTGAFVVVVPVVTNFKVNWDSTSNAWVCNIT
                     TGITFIDAAHDAKVISIESIDTSGSDKKNPNDAVKAAIDAIPAELEYRIRSIPEFQIS
                     TRILEVSGSTVKIQLGSNMGLKKGDEYAIVEIRNVGGIQDTQETGLIMITDVSSQIST
                     GRILYKSGSVGQNTQLKEIAKYGSDLDLYFHMSGKEMLPGLRATIARGFFPFRPYVGI
                     QMPIDLAFNFFSVTAIPVNVAIGGEYMIPLGRLHVAPYVGAGITYLYITEAITGYTNT
                     NYLSHYGFQAGARIGYLINRNMRVFLDAGYDYWASLDSGLFPNYGGLTIGAGVTFKL"
     gene            2657..4498
                     /locus_tag="PLQ30_07135"
     CDS             2657..4498
                     /locus_tag="PLQ30_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPW02257.1"
                     /translation="MKRFFMVLCALLALTFGLSAQSKSVDLTFIETSDIHGSIYPYNF
                     ITAKPAGTSLAQVASLIKEERSVPDAEVVLLENGDSLQGQPTVYYYNFEKTDGPHIWS
                     QAVNFLNYDAVSVGNHDIEAGHAVYDKLYEELQAPVICANAIKPDGTPYFEPYAVIEK
                     AGIKIAILGMITPKIPDWLPPQFWTGMQFEDMVKCAQKWVPIIQQKEKPDLLIGLFHA
                     GVDYSYGNVGRDTPNNENASQLVAEMVPGFDLIFVGHDHQGWSGQGWDPANKTKVDVK
                     DPNGKIVPIYGALNAARNVAVVKMNLTWNDETQSWDKIVRGGLVDMSKYQPDPDFMTK
                     FQPMFDEIKAWVDRPIGKMEGVISSRDSMFGDSAFVDLIHIIQLELTKDPTLGLAPAD
                     VSFAAPLFADAQIPSSPDGTLYVRDMFNLYVYENFLYTMNLTGQQIKDFLEYSYQYWF
                     DTMPNEGNHLIAFQRDKEGKLILDNRTNMPMTATRYYNYDSAAGINYIVDVSKPAGER
                     VSIYCMSDGRAFNPKAKYTVAINSYRASGGGGHLEKGAGLDKDTILSMKLVNGATTKD
                     LRYFLLKWFEKQEETITVWPLGNWEVVPEDLAAIGRANDYPLLYPSK"
     gene            4739..5299
                     /locus_tag="PLQ30_07140"
     CDS             4739..5299
                     /locus_tag="PLQ30_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPW02258.1"
                     /translation="MNKRPYMDIGAIFDDIFEAAKDFGERVKNFSPGFGGPNQEGEES
                     RPGCHGMGGAWFEAHGDDNVDYYPNYSYPPANVYMLPDKSLVFEFALAGFDEKNISLS
                     FQGDYMVFSAKIDAEKPEEGVRYFKRRLRLKDIDKQKYYVPADKFDQAKVKAAFRNGI
                     LKITVPPKDVVDIPEGIKIEIIKEGE"
BASE COUNT         1476 a         1291 c         1230 g         1462 t
ORIGIN      
        1 tgattctttt cttctgcatc tcctttcttg aagatacagg ctacattgct cgcgctgcct
       61 ttttgaccga tcattttctc cacgcaatag ggctgcatgg acagtcgttc atgccgctca
      121 tgcttgggtt tggctgttcg gtgccagcta taatggccac ccgtacgctc aaaagcccca
      181 aagagcgaat cgccacgatt ctggcaattc cctttatttc ctgcggcgga aagctgccaa
      241 tctatgtgct cttggccggg acttttttcc caaatcatga atcgactgtc gttatgctgg
      301 tgtacgccac cggagttgtt ttgagcattc tttcgaccta ttttcttagg cgaacggtgt
      361 taaaaggcca atctatgcca tttgtcatgg agctgccgcc ctatcgcatg cccacgctaa
      421 aaggagtgct ctggcatgtc tgggaaaaga catggagcta tgtgcgcaaa gcgggtacga
      481 ttatcctcgc cgtatctgta ctcatgtggg ttattacatc gtatccaaaa gccaagggga
      541 ttgatgacta tgcgctgcaa gtgcgccagg agattgccac cgctcatcct gattttgatg
      601 aatcgctgat tgctgcaaat gttgagatac tccttcgtca atatcagctc gaacggagta
      661 tcgcgggaaa cataggcaag ataatcgagc cggtaattgc gcccatcggc ttcaattgga
      721 aaattggaat tgcgctccta ccaggcctaa cggcaaagga acttgtggtc tctacattag
      781 gtgtactcta tggtacgaca gtaaatgatg aaacagaggc gaattcttcg ctgcgccaag
      841 ctcttcgcaa ttctccagac tggtccccgc gtatagccct tgctcttctg atttttatcc
      901 tggttatgcc accatgtttt tcatctttgg ctgtaatacg caccgaagcc ggtgacaaat
      961 ggctagcttt tcaagttgta tactcgcttg tcctggcttg ggtgctatcc ttccttgcct
     1021 cgatggcagg gcacctctta ggattgggct gaggtctgtt agagcttgaa tgtcactccc
     1081 gcaccaatcg ttagccctcc gtaatttgga aacaagccag aatcaagaga tgcccaataa
     1141 tcatatccag cgtctagaaa tacacgcata ttcctgttga tcagatatcc aatccttgca
     1201 cctgcctgga agccataatg agatagataa tttgtgttcg tatagccagt aatagcttct
     1261 gtaatataca aatatgtaat gccagcacca acatatggtg ccacatgaag cctcccgagc
     1321 ggaatcatgt attcgccacc aatcgcgaca tttactggta ttgctgttac gctaaagaaa
     1381 ttgaatgcga gatctattgg catctgtata ccgacatagg gcctgaatgg gaagaacccc
     1441 ctggctatgg tcgctctcaa acccggaagc atctctttgc cactcatgtg aaaatacaaa
     1501 tcgagatctg aaccatattt tgcaatctct ttcagctgcg tattctggcc gaccgagccg
     1561 ctcttataga gtatgcgccc tgtggaaatc tggctgctta catctgtgat cattatcagt
     1621 cctgtttcct gcgtatcctg gatgccgcca acattccgga tctctacaat ggcatattca
     1681 tcgccctttt taagtcccat gttgctgcca agttggattt tgaccgtaga acccgaaacc
     1741 tccaatattc gcgtactgat ctggaactcg ggaattgatc tgattctata ttcgagctca
     1801 gctggaatcg cgtctattgc ggccttaacc gcatcattag gattcttctt gtcgctgccc
     1861 gaggtatcga tcgattcgat tgagataacc tttgcatcgt gtgctgcatc gatgaaagtt
     1921 atgccagtgg ttatgttgca tacccacgcg ttggaagtgg aatcccaatt gaccttaaaa
     1981 ttcgttacaa caggcaccac aacgacgaaa gctccagtaa gacgctcgaa ctccgctttt
     2041 gtaagaaagg cctcaccaaa ttgatactgc tccggaagca cgaaattggc ttcttttgac
     2101 ctgcgcagtg tatcgataaa ttcttgtacc ccgttcgatg agagtcgctg tgttgttcca
     2161 aaaacattga atctgccaat cttagcgaac acttcctgaa tcttgagatc gattgtaccc
     2221 aatgcctgag atggaataag ccatccataa tagcccaaag caaatacagc aatgtcttgt
     2281 ttttggctta cgacaggttg tgcttgtgta ctcgcagact gcgcacttgc agattgccca
     2341 gctgcaccaa ataatattgc tccaatgaat agcaatgcaa aaaacaaagc atatctagac
     2401 gatctcatac gataaccctc ctttttgggc aatcctttta agcgaaacta tatgatgaat
     2461 tataggataa gtactctaga gaagtcaaac aaattattgg tgctgctcac cttaagtgga
     2521 cagttctctt tacaggagac cgtgctgctc accatgctgc tcacccttga atgtcattct
     2581 tgaaaatcat gttgcgctct ccttgaaatg ggcttatcat tattatgctc aaaaaaacca
     2641 tagataagga ggtcttatga aaagattctt tatggtgctc tgcgccttgc tcgctctgac
     2701 tttcggactc tcggctcaga gcaaatcagt cgatctgaca ttcatcgaga caagcgatat
     2761 tcatggctcg atttatccgt acaatttcat taccgcaaag ccagccggaa catcgcttgc
     2821 gcaggtggca agcctcatca aagaggaacg ttcggtacca gatgctgagg tggtgcttct
     2881 tgaaaatggt gattctcttc aggggcagcc cacagtctac tactataact ttgaaaagac
     2941 cgatggtcct catatctggt ctcaagcggt gaattttctc aactacgatg ctgtgagtgt
     3001 aggcaaccat gacatcgagg ctggtcatgc agtctatgac aaactctatg aggagctaca
     3061 agcgccggta atctgtgcaa acgccatcaa acccgatgga acgccctatt tcgaacccta
     3121 tgctgttata gaaaaagctg gaataaaaat agcaattctg ggcatgatca cacccaaaat
     3181 cccagactgg ctacccccac agttctggac cggcatgcaa tttgaagaca tggtcaagtg
     3241 cgctcagaaa tgggtgccca tcattcaaca aaaagaaaaa cccgaccttc ttattggcct
     3301 tttccatgct ggcgtggatt attcctatgg aaatgttgga cgcgatactc cgaacaatga
     3361 aaacgcctcg cagctagtag ccgaaatggt acctggtttc gatctgatct ttgttggtca
     3421 tgatcatcaa ggctggtctg gtcagggctg ggatccggct aataaaacaa aggtcgatgt
     3481 aaaagatcct aatggcaaga tagtgcctat ctatggagct ctaaatgcag cccgcaatgt
     3541 agctgttgta aagatgaacc taacatggaa tgatgaaacg caaagctggg acaaaatcgt
     3601 ccggggcggt cttgtcgata tgagcaagta ccaaccagat cctgacttta tgaccaaatt
     3661 tcagcccatg ttcgatgaaa tcaaagcatg ggtcgaccga cctataggca agatggaagg
     3721 tgtcatttcg agtcgcgatt cgatgttcgg agattcggcc tttgtcgatc taatccatat
     3781 aattcagctc gaactcacta aggacccgac actcgggctt gctccagccg atgtttcctt
     3841 tgccgctcca ctgtttgcgg atgctcaaat tccttcgagc ccggatggaa ctttatatgt
     3901 gcgcgacatg ttcaacctct atgtctacga gaacttccta tacaccatga atctaacagg
     3961 acaacagatc aaggattttc ttgaatactc ctaccagtac tggttcgata ccatgcccaa
     4021 cgagggtaac catctaatag ctttccagag agacaaggag ggcaagctta tactggacaa
     4081 tcgaaccaat atgcccatga ctgccactcg ctactacaac tacgattcgg ctgccggaat
     4141 caactatata gtcgatgtca gtaaaccagc cggcgagcgt gtatcgatct attgtatgtc
     4201 cgatggccga gctttcaacc ccaaggcgaa atatactgta gctatcaact cctatcgtgc
     4261 atcaggcggc ggcggacatc tggaaaaagg tgcaggtctg gataaagaca cgattctctc
     4321 catgaagctc gtaaacggag ccacaacaaa agaccttcga tatttcctcc taaagtggtt
     4381 cgagaagcag gaggaaacga ttacggtatg gcctctcgga aactgggaag tcgtcccaga
     4441 agacctggcc gcaataggca gagcgaatga ttatccgctt ctctatcctt cgaaatagag
     4501 attgtctgct ttgagctcag gcaggggctg cccaagttaa gcggcagccc ctttttatta
     4561 tggattgatt ttattatgga ttgaatcata gctttttagt atcgatgctt cttcttgatt
     4621 ttatgagtct ttatttctat attgaaatca agaaaagagc ttgcccgcat gggaagctat
     4681 cacaaagccg aggggcataa gccgcggcgt caaaagattc ccgaaaggag atagaaaaat
     4741 gaataagaga ccttacatgg acattggagc tatcttcgat gacattttcg aagcggccaa
     4801 agattttggc gaaagagtca agaatttctc acctggcttc ggaggtccaa atcaggaagg
     4861 cgaagagtca aggccggggt gccatggcat gggtggtgca tggttcgagg ctcacggaga
     4921 cgataatgtg gattattatc ccaactattc gtatccgccc gctaatgtct atatgttgcc
     4981 cgacaaaagc cttgttttcg aattcgcctt ggcaggtttc gacgaaaaga acataagcct
     5041 atctttccag ggagattata tggtgttttc cgctaagatc gatgctgaga aaccagaaga
     5101 aggcgtacga tacttcaaga gaagacttag gctaaaggat atagacaaac aaaagtacta
     5161 tgtccccgct gacaaattcg atcaagctaa ggtaaaagca gcctttcgaa atggaatatt
     5221 aaagataacg gttccaccca aggatgtagt cgatatacct gaaggtatca agatcgaaat
     5281 aataaaagaa ggcgaataac attggtagcc tgaaaccgtg gacaagaaat aacctttgat
     5341 agcaatcgaa tagcctttga gagaagcgcg atcctcacag accgcgcttg ttttgtctac
     5401 ttgttttgtc tacttgtttt gtctattcga acaaaccgct gcctcttaaa gactgcctc
//
LOCUS       DAOMZL010000096         5374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_14348_length_5374_cov_14.272608, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000096 DAOMZL010000000
VERSION     DAOMZL010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14348_length_5374_cov_14.272608"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..1316
                     /gene="manA"
                     /locus_tag="PLQ30_07145"
     CDS             63..1316
                     /gene="manA"
                     /locus_tag="PLQ30_07145"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HPW02259.1"
                     /translation="MNEEDDTIDAQRQRFQCIGLLKNSAQQYVWGDTQGITPFVEAHP
                     VPGYPIGEVWMGSHERAPSKLIFNNSELALDRLIRQDPTYWLGDHISTKYDDLPFLFK
                     VLAAGSPLSLQVHPNLEQAEIGFSREEAAGIGRLTPERTFKDPHHKPELAVALTSFKA
                     LAGFRPIEEICYLLGHRLCDLLDFVGKDRSELRSLIRRLFRIRGEEYSAFETELNYRA
                     KQLMYSKQEAVRDAAHWVLELQSRYPGDPGQFAPLFLDLLQLEPGQGLFVPAGVIHSY
                     LKGSILEIMACSDNVIRAGLTSKHMDVDLLCDILDPETKPMLISPEYQRFEWGTLAIY
                     KTPAKEFRLEYFEANSDASFFPIAIASKGPKILLCIKGSFRLCTREQIELSARKSCFI
                     AGSCEKIELRGQGKLWLATVGEIDT"
     gene            1313..1900
                     /locus_tag="PLQ30_07150"
     CDS             1313..1900
                     /locus_tag="PLQ30_07150"
                     /inference="COORDINATES: protein motif:HMM:NF014676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HPW02260.1"
                     /translation="MNSVSHPELTGSNLGQPSFSEEHGSDPTITIWVDADSLPKDIRP
                     MLIRRAMAKRKYDGVTILVRFVASQNLLEIPGDYLILVQPGEGAADLYIETHAEQNDI
                     AITRDIPLAERLLAHSVNPMNDRGDIFTAENISERRSLRDAMAYMRDSGIAPPSPKGN
                     LRKPSDIKSFADGLEKLIVQSVRSKRRCLENPPRS"
     gene            complement(1897..2985)
                     /locus_tag="PLQ30_07155"
     CDS             complement(1897..2985)
                     /locus_tag="PLQ30_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPW02261.1"
                     /translation="MKPESQSSEARALKVKKNGKARKPVWKILRIALLAILIVHLVFI
                     GLTSALIFAYKFANPPTTVLIIYRSVVSHWEVQKPIPLKTEQIPQYIRRMLISVEDGG
                     FYEHHGIDIEAIKRARELNEKVGKPMYGGSTITMQVARTLFLLPTKSYIRKYFEAITA
                     LELELILSKDRILELYFGYAEWGKGIFGIERASRVYFGKGVASINRDQAARLIALLSS
                     PIKYTPDTLYRSAILRQRYAYLVARYVAAVPVAVVSAETPPPSGIEPSADISELSDEE
                     EDSKTEQEVSPNDGELLEDTSSDLSPIDSSPTDSSPIDSDDGASGDTSSDLSPMDSVE
                     PSPEIDNLQETKDKEKADEPSISGTPEF"
     gene            complement(3003..3539)
                     /locus_tag="PLQ30_07160"
     CDS             complement(3003..3539)
                     /locus_tag="PLQ30_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPW02262.1"
                     /translation="MTEKTFMTGFGGQGIISMGQLWVYCAMKEGLKVTFFPFYGAEKR
                     GGIARASVIVSDSEITSPIVSVADSVVIMNQDSVEIGEKVCKEGGIICVNSSLVKKDP
                     ARADAKIYHIPCNEIALKLGNIRVANMIMMGALSKITGAVKLQDLENILPNYFSSSKR
                     SIIEINIAAVEEGKKVVA"
     gene            complement(3552..4325)
                     /locus_tag="PLQ30_07165"
     CDS             complement(3552..4325)
                     /locus_tag="PLQ30_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPW02263.1"
                     /translation="MEIAYGHPKSLKSIQTKYCPGCGHGVIHRIIAEVVDEMGLQSKT
                     IITNPVGCSIYADMYFDFDSVQPAHGRTPAASTGIKRMLPDHLVICYQGDGDLAAIGT
                     AEIIHAANRAEKFTTIFVNNAIYGMTGGQMAPTTLVGQHATTAPEGRDPINAQMGYPI
                     RVCELLASLEGTKYLARGAVNNMVNIRRTKKYIRKAFEAQMRGEGFSLIEILSQCPTN
                     WQMNPVESVEWLEKNMIPYYHLGEIKDTLSTTVAPAQAH"
     gene            complement(4341..>5374)
                     /gene="vorB"
                     /locus_tag="PLQ30_07170"
     CDS             complement(4341..>5374)
                     /gene="vorB"
                     /locus_tag="PLQ30_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPW02264.1"
                     /translation="AIGEAAVRAGCRAYFGYPITPQNELTAYMAKHMLARGRTFIQAE
                     SEVAAINMVYGAASTGARAMTSSSSPGVSLKQEGISYLCGADLPAVVIDVARAGPGLG
                     GISPAQGDYFQATRGGGHGDYYTIVLAPKGVQEAADLTYEAFGLAEKWRMPVLILADG
                     LIGQMMEGVVLPEPMDPANLPRKSWTVGHSVEMARPFNHISSINLIPDELEAINRKRF
                     ERYAQVKAELIRYEELEVEDADIVFVAYGTSARVSHGALQMARAKGMKIGLFRPITLW
                     PFPYKRLSELAEQGKRFLCVEMSMGQMIEDVRLAVGGKAPVYFYGRCGGNIPSQEEVF
                     AEALRLSSK"
BASE COUNT         1384 a         1331 c         1226 g         1433 t
ORIGIN      
        1 gctctggact ctatttgtcg cgatgtggca gaattccttg ataatgaaag gtaatcgatt
       61 cgatgaacga agaagacgat acaattgatg ctcaaaggca gcgtttccag tgcataggac
      121 tactgaaaaa ttctgcccaa caatatgtat ggggcgatac acaagggatt acgccctttg
      181 tggaagcaca tccggtaccg ggttatccta ttggggaggt ttggatggga agccacgaaa
      241 gagcaccctc taaactaatt ttcaataatt cggaactggc tcttgataga ctaataagac
      301 aagatcccac ttactggctt ggagatcata tttctacgaa atatgatgat ttaccttttc
      361 tattcaaggt actggctgcg ggttctcctc tatcccttca ggtccatcca aacctagaac
      421 aggcggagat aggattttca agagaagaag ctgccggcat tggccgattg actccggagc
      481 gaacattcaa ggatccgcac cataaaccag aactagccgt ggcacttaca tcatttaaag
      541 ctctggctgg tttccgcccc atagaagaaa tatgctatct tttgggtcat aggctatgcg
      601 atttactcga ttttgtagga aaagatagaa gtgagctccg tagcttaata aggcgtttat
      661 ttcgcattag aggtgaggaa tactcagcct ttgagaccga attgaattat cgagccaaac
      721 aactcatgta ttcgaaacaa gaggccgtcc gagatgctgc acattgggtg ttagagcttc
      781 aatcgcgcta tccaggtgat ccaggacaat ttgctcccct ttttctcgat ttgctgcagc
      841 tggaacctgg ccagggtcta ttcgtaccag ctggtgtgat tcactcttac ctgaagggca
      901 gtatccttga gattatggct tgttcagata atgtcataag ggcaggtctc accagtaagc
      961 atatggatgt cgatcttcta tgcgatatcc tcgacccgga gacgaaaccc atgttaatct
     1021 ctcccgaata ccaacgattc gaatggggaa cccttgccat ctataagact ccagcgaagg
     1081 aattccgcct tgagtatttc gaggcgaatt ccgacgcatc gttttttccg atagcaatcg
     1141 cttctaaagg gccaaagatt cttctatgca tcaaaggaag ctttagactt tgtaccagag
     1201 aacaaatcga attgtcagct cgcaaatcat gctttattgc aggatcctgc gaaaagatcg
     1261 aactgagagg acaaggcaag ctctggcttg ctaccgttgg cgagatagac acatgaattc
     1321 ggtttcgcac ccagagctaa caggctcgaa tctgggtcag ccctccttct cagaggagca
     1381 tggatctgat cccacaatta ctatctgggt ggacgccgat tcacttccaa aagatatcag
     1441 gccaatgttg ataagaaggg ctatggcaaa aagaaaatac gatggtgtta cgatccttgt
     1501 gcgcttcgta gcaagccaaa accttctgga aattccagga gattatctca tccttgtcca
     1561 gcctggcgag ggagctgctg atctctacat tgaaactcat gcagaacaga acgacatcgc
     1621 cataacccga gatatccctc tcgcagaacg ccttctagcg cattcggtta atccaatgaa
     1681 tgatcgcggc gatatcttta ctgcagagaa tatcagtgaa cgacgttctc ttagagatgc
     1741 tatggcttat atgcgagatt cgggtatcgc tccacccagc cctaaaggca acttgcgaaa
     1801 gccatccgat ataaagagct ttgcggatgg tctcgaaaaa ctcatagtcc aatctgtgag
     1861 atcaaagaga cgatgcttag agaacccacc caggtcttaa aattcaggcg tccctgaaat
     1921 cgatggttca tctgcttttt ctttgtcctt agtctcttgc agattatcta tctccggaga
     1981 gggctcaaca gaatccattg gggataaatc cgaggatgta tcgccagatg ctccatcatc
     2041 agaatctatt ggagatgaat ctgttggaga tgaatctatt ggagatagat ccgaagatgt
     2101 gtcctctaat agttcaccat catttggaga gacctcttgt tctgttttag aatcctcctc
     2161 ttcatcggat agctccgata tatcggccga aggctctatt ccggatggtg gaggtgtctc
     2221 ggcagagaca acagctacag gcactgctgc tacatatctc gcaacaagat aggcataacg
     2281 ttgccgcaag attgcagagc gatagagagt gtcgggtgta tattttattg gagaagacag
     2341 aagggctata aggcgagccg cttgatcccg attgatactt gctacacctt tgccaaaata
     2401 gacccgagaa gcgcgctcga ttccaaagat ccctttaccc cattccgcat aaccaaaata
     2461 gagttcgagg atgcgatctt tcgagagaat caattccagc tctagcgcag tgatggcttc
     2521 gaaatacttg cgtatatagc tcttagtagg caaaaggaaa agggtacgcg caacctgcat
     2581 agtgatcgtg cttccaccat acattggttt ccccactttt tcgttgagct ctcgtgctct
     2641 tttgatagcc tcgatatcga tgccatggtg ctcatagaat cccccatcct ctaccgatat
     2701 tagcatcctt cttatatatt gaggaatttg ctcggtcttt agagggatgg gtttttgcac
     2761 ttcccaatga cttactaccg atcgataaat aatgagcact gttgtgggag gattggcaaa
     2821 cttatatgca aatattaatg cgctcgtcaa acctataaat actaaatgca caatgagtat
     2881 tgccaatagg gctatacgga gtattttcca aaccggcttt cgagctttgc cgtttttctt
     2941 tacctttagt gctcttgctt ctgacgactg cgattcaggc ttcataaggt aagagcatta
     3001 tatcacgcta ctaccttctt tccttcctca actgcggcaa tattgatctc aatgattgag
     3061 cgcttactcg aagaaaaata gtttggcaat atgttttcta ggtcttgtag cttaacggct
     3121 cccgtaatct tgctcaaggc tcccatcatt atcatatttg ctacacggat attgccgagc
     3181 ttcagtgcta tctcgttgca tggaatatga tagatttttg catcagcacg ggcaggatct
     3241 ttcttgacga ggctggaatt gacacagatg attccgcctt ctttgcaaac cttctctcca
     3301 atttccacgg agtcctgatt cattatcacg actgaatcgg caacgctgac aataggactt
     3361 gttatttcgc tgtctgagac tatcaccgat gcgcgtgcta ttccgcctcg tttctcggct
     3421 ccatagaagg ggaaaaacgt cactttgagg ccttctttca ttgcgcaata aacccaaagc
     3481 tgccccattg aaataatacc ctggccaccg aagcctgtca taaaagtctt ttcggtcatg
     3541 gaacactcct ttcaatgcgc ctgcgcaggg gctactgtcg tgctgagagt atctttgatt
     3601 tcgccaaggt gataataggg aatcatgttt ttctcaagcc attcgacaga ctccacggga
     3661 ttcatctgcc agtttgttgg acactgtgag agaatctcga tcagggaaaa accttcgccg
     3721 cgcatttgag cttcgaaggc cttgcgtatg tatttctttg tccttctaat gttgaccata
     3781 ttgtttacgg cgccgcgcgc aagatatttt gtcccttcga gcgaagccaa gagttcgcat
     3841 actcgaatag ggtagcccat ttgagcattg ataggatcgc ggccttccgg cgccgttgta
     3901 gcatgctgtc cgacaagagt tgtaggagcc atctgccctc ctgtcatgcc atagattgca
     3961 ttgtttacga agattgtggt gaatttctca gcccggttag cggcatggat aatctcggct
     4021 gtcccaatgg ccgcaagatc accatcgcct tgatagcaga ttacgagatg gtcaggcaac
     4081 atccgcttta ttcccgtcga agcagctggc gtccggccat gtgctggctg aactgagtcg
     4141 aaatcgaaat acatatcggc ataaatagaa cagccaaccg gattcgtaat aatcgtctta
     4201 ctttgcaacc ccatctcatc tacgacctcg gctatgattc tatggattac tccatgaccg
     4261 cagccagggc agtattttgt ctgtatcgat ttaaggctct taggatgccc ataagcaatt
     4321 tccataataa atccctcccc tcatttgctc gaaagtcgta gcgcttcggc gaaaacttct
     4381 tcctggctgg gtatattccc gccacagcgc ccatagaagt acacaggagc ttttccaccg
     4441 accgcgagcc gtacgtcctc gatcatctgc cccatgctca tctcgacaca aagaaagcgc
     4501 ttgccttgct ctgctagctc agaaagccgc ttataaggaa atggccagag tgtaataggt
     4561 cggaaaaggc caatcttcat ccccttggct ctagccatct gtagagcacc gtgtgaaacg
     4621 cgcgctgaag ttccatatgc tacaaagact atatcagcat cctcgacttc cagctcctca
     4681 taacgaatta gttccgcttt tacctgtgca tagcgttcaa atcgcttcct gttgatggct
     4741 tctagctcgt cgggtatgag atttatgcta gatatatggt taaaaggcct tgccatctcc
     4801 acagagtgcc cgacagtcca ggatttacgc ggcaaattgg ctggatccat gggctccggg
     4861 agtaccaccc cctccatcat ctgaccaatt agcccatccg cgagaataag cacaggcatg
     4921 cgccatttct cggcgagtcc gaaagcctca taggtcagat ccgcagcctc ctggacaccc
     4981 ttgggtgcga gaacaatcgt ataataatcc ccatgcccac cacctcgggt cgcctggaaa
     5041 taatctccct gagcaggcga tataccgccc aggccagggc ctgcacgtgc aacatctatg
     5101 actaccgctg gaaggtcggc gccgcagaga tatgaaatac cttcttgttt aagtgaaaca
     5161 cccgggctcg aggagctggt catcgcacga gcacctgtgg aagcagcacc atataccata
     5221 ttgattgccg caacttcaga ctctgcctgg atgaaggtgc ggcctctagc tagcatgtgc
     5281 ttagccatgt aggcggtgag ctcgttctgc ggggttatag gataaccaaa ataagcgcgg
     5341 caccccgctc gcaccgccgc ttcgcctatc gctt
//
LOCUS       DAOMZL010000097         5298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_14611_length_5298_cov_17.755865, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000097 DAOMZL010000000
VERSION     DAOMZL010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14611_length_5298_cov_17.755865"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PLQ30_07175"
     CDS             complement(<1..471)
                     /locus_tag="PLQ30_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW02265.1"
                     /translation="MKEDIEQKEKAQGGTPERLKVGGMFAYGAASVGMGLFYAFNNFS
                     LPLFYRTYTSSDALIGLLANTRSFMSAIVQPFVGAWSDRTWTKMGRRKPFFAFSMPLV
                     AVLLLWCAARPPFGVLIAVQLILTFLFNVGVDPYTALISDIAPENQRSTVNSVAT"
     gene            complement(852..2399)
                     /locus_tag="PLQ30_07180"
     CDS             complement(852..2399)
                     /locus_tag="PLQ30_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW02266.1"
                     /translation="MTKKEHSKPLAKQVPSPEGGPALTSISRASLTTERPVTFKTLIA
                     YGVGDIYGGGAFIIVSLFYMFFMTEVVGLSPALAGLALGIGKVWDAITDPLMGYLSDH
                     TRSRYGRRRVYFLVGIVPIALSYIIMWIRVRASSQVALFFYYLFAYILFDAVITMVMT
                     PYSALAAELSADYKVRNRLSAARLFFSGFSSLLASTVPSMIINAHKEDPAQGYLIMSI
                     VFGILFAIPWIFVFWGTWENPHLASPTVSRDFMKEFASILKNKSFRIHLMMYVFAYSA
                     MDMLMGLFTYYLTYYLQRPNIYSIAMGTLMIVQLFMILVYTKVANTKGKRFAYILGMS
                     IFLAGLYATIVLTSSTPLLVLVLICAVIGLGTAAAVYIPYAILPNVIDVDELMTGQQR
                     AGVYSGAMTLARKVVQGIIVMPLIGFFLQLVGFQSNQAQTPEVLSKFLIFFITGPSIF
                     LILGIIVAMRFELSPKNSEILVKELERIRAGGRLEDVSPEVKTICEKLTGMRYEECWN
                     KKGATEI"
     gene            complement(2677..4989)
                     /locus_tag="PLQ30_07185"
     CDS             complement(2677..4989)
                     /locus_tag="PLQ30_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02267.1"
                     /translation="MNFYHSYRGDTPDERGFGKDIRVIRSILDSLDELSKDGIEVKCA
                     WDFDNAFTLGHILPSFAPDIIERIKMRVIAGVDEIELMSWNNGLLTAHTPEELRYALS
                     WAINAPDGSGVLQTFGSYVPIARPQECMFTGSHIDIYRSVGIEAISLYYSAIPFNAIG
                     SFISELEARKRYNPLTLKNPTTGASIRLIPAINQGDLAEYYFSAARMLKAIRAEQLHE
                     TFRCAHGLDAEGDPERDIEMGIEGDSGGIAQPVDMLVVLDIDADDSFWAGFLPGFSRR
                     FVPSFGGLYSLISSIARLPFVGFERPWEYIKTHPDRGEIVIGQDMADGAFDGYSSWSE
                     KYENYEIWSIIAEARSQWQSAKSFVAVRNGESVLRSATSPKTETYWLDELSELDPSLA
                     RLAHEAIKERLRALSTTHFGLAVPVMNVTRLEIARETANRSLELAKAFYTAVKHKYGV
                     VNAISNLPCIKEPQELRGPELPQAAHPESRVRIQDSSPHSPHLTLSVSSKGRIRLASG
                     SYTVHINEPWVEYNHKILGSRKIDVISGAERYELHGIIPLRPMRVAIEWSRTATMLSA
                     DLVCFELHVQYPSTPHRCYDHKKARTLGRTWDDRWRQLAPFEIVAFENLPCSNIIHVW
                     KEDFSNVISHYSLDYAGYGRNRFLADINNHLTPKWLAVSDGARGILVAQASQSFSSYA
                     FCPLRQDLRCGVQCVSMFPFGALWGPQYRYPAAVTGLGRRAAILTAEHLHSSAPSWEG
                     KTLDARLLIALYEGNEPQRSLLAKVHECLL"
BASE COUNT         1465 a         1265 c         1197 g         1371 t
ORIGIN      
        1 ggtggcaacg ctattcaccg tagaacgctg attctctggg gctatgtccg aaatgagagc
       61 agtataggga tcaacgccga cattgaaaag aaaagtgagt atgagctgca cagcgatgag
      121 aacgccaaat ggcggccgcg ctgcacacca tagaagaagc acagccacta gaggcatgct
      181 gaatgcgaag aagggcttcc ttcggcccat ttttgtccat gttctatcgc tccacgcgcc
      241 cacgaaaggt tggacaattg cactcatgaa tgaacgggtg ttcgcaagaa ggccaatgag
      301 agcgtcgctc gaagtgtagg ttctataaaa gaggggaagg ctgaaattgt tgaaggcata
      361 gaataagccc atacccacgc tggccgctcc ataggcgaac atacccccga ctttaaggcg
      421 ttcaggcgtc cctccttgag ctttttcttt ttgctcaata tcctccttca tatctacctc
      481 ctcatatcat ttcccgtgcc acttagctca ttctatcttg agtttattgc gcgatgtggc
      541 cagcgtcaaa tccattttgc tcgagatatc gcgagagcta ctaattatgc aaaggaatta
      601 taatctgatt cgatgtgggt ggcttgaacg aatctgctat gccgcattac tttggacagc
      661 aattcgtcca tgataggttt tttcgccgac ttttgcatgc tcttcctttt ctctagatac
      721 tataagttat tataggaata tagaatatag aagccagcat gcaaatctac ttgttgatac
      781 gatatttgca gatgaaattg ctatcaattc aaatgtttgc acgaattcct gaggatgctt
      841 ctgaaatatt gtcatatctc agttgcgcct tttttgttcc aacactcctc atagcgcatg
      901 ccggttagct tctcgcagat ggttttgact tctgggctga catcttccaa cctccctcca
      961 gcgcggattc gttcaagttc tttgactagg atctcgctgt tttttgggct gagctcaaag
     1021 cgcattgcga cgatgatgcc caaaataagg aaaatactag gcccggtgat gaagaatatc
     1081 aagaatttac tgagtacttc cggcgtctgc gcctggttgg actgaaagcc aacgagctga
     1141 aggaagaatc cgatgagcgg cataacgatg ataccttgaa caacctttcg cgcaagagtc
     1201 attgcgccag aatagactcc agcgcgctgt tgacctgtca ttagctcatc tacatcgatt
     1261 acattgggca aaatggcata tggaatatag accgcagcgg cagtcccaag ccctatgacc
     1321 gcacagataa gtacgagaac tagcaatggc gtagacgatg taagaacaat agtcgcatag
     1381 agaccagcaa gaaagatcga catgccaagg atatacgcaa agcgcttgcc cttggtgttc
     1441 gcaaccttgg tatagacgag aatcataaaa agctgcacga tcatgagcgt ccccattgcg
     1501 attgaataaa tattcggcct ttgcaaataa taggtaaggt aatacgtaaa gagccccatc
     1561 aacatatcca ttgcagaata ggcgaaaaca tacatcataa gatgaatccg aaaggatttg
     1621 tttttaagaa tgctcgcaaa ttccttcata aaatctcgcg atacggttgg gcttgcaaga
     1681 tgtggattct cccatgttcc ccaaaaaaca aatatccagg gaattgcaaa tagaattcca
     1741 aacacaatcg acatgataag atagccttgc gcaggatctt ccttatgtgc attgatgatc
     1801 atgctcggca cggtcgatgc cagcagcgat gaaaagcctg agaaaaagag cctcgcagca
     1861 gagaggcgat tacgcacctt ataatcagcg gatagctcag cagcgagcgc agaatagggg
     1921 gtcatcacca tcgtgattac cgcatcgaag aggatatagg cgaagagata ataaaagaaa
     1981 agcgcgactt gcgagcttgc tcgaacgcga atccacatga ttatgtaaga aagcgcgatg
     2041 ggcacaatac ccacaaggaa ataaacgcgc ctgcggccat aacgcgaacg tgtatgatct
     2101 gaaaggtagc ccataagagg gtcggtgata gcatcccaaa ccttcccaat accaagcgca
     2161 aggcctgcaa gggcaggaga aaggcctacg acttcggtca tgaagaacat ataaaaaagg
     2221 cttacgatta tgaatgcgcc acctccatat atatcgccga ctccataggc gataagcgtc
     2281 ttaaaggtaa cgggtctttc ggtagtgagc gaagctcttg aaatagaagt cagagcaggt
     2341 ccaccctccg gagatggcac ttgcttggcg agaggcttcg aatgttcttt ttttgtcata
     2401 ctctgcctcc ttgcttttgc cggcagtatc ttacgcctca tgttcccaaa ttatgtgcac
     2461 tgagattggt caaatcgatt ttaccggatc ttacttgtgc tgtcatctat gcatcgcaaa
     2521 gaatgggttt cctttcttcc gtgctcattt gtctgcaata ttgtattgat atcaggcatt
     2581 agcaaacgta catttttgct tccgactttt ttggaatgaa ttctttggaa tgaaagagaa
     2641 tagatacatc tcatgacgct ttgattttcg cctttatcag agcaagcatt cgtgcacttt
     2701 tgctagaaga cttctctgcg gttcattacc ctcatagagt gcgataagaa ggcgcgcatc
     2761 aagggtcttt ccttcccatg agggggccga ggaatgcaga tgttccgcag tcagaatggc
     2821 agcgcgcctt cctaagccag ttaccgcagc aggatatcgg tactgtggtc cccaaagagc
     2881 accaaaaggg aacatgctca cgcattgtac accacaccgt aaatcctggc gtagaggaca
     2941 gaaagcataa ctgctaaaag attgagaagc ctgcgcaact agaatccctc gcgcgccatc
     3001 gctcactgca agccattttg gcgtaaggtg gttattgata tctgcaagga accgatttcg
     3061 accatagcca gcataatcta gcgaatagtg cgaaatgaca ttactaaagt cctctttcca
     3121 tacatggata atgttagaac agggcagatt ttcaaatgcg acaatctcaa aaggcgcaag
     3181 ctggcgccag cgatcgtccc atgttcgacc gagtgttctt gcctttttgt gatcataaca
     3241 tcggtgcggg gtcgatggat attgcacatg aagctcgaag cacacaagat ccgcgctgag
     3301 catagtagca gtcctgctcc attcgatcgc cactcgcatg ggccttagag ggatgattcc
     3361 atgcaattca tatcgctcag caccggatat aacgtcaatt ttgcgcgaac cgagaatttt
     3421 atgattatat tcaacccagg gctcgttgat atgaactgta tatgatccac tggctagccg
     3481 gattctgccc tttgagctga ccgaaagcgt gagatgagga ctgtgaggag aagaatcttg
     3541 tatgcgaacc ctgctttctg gatgcgctgc ttgaggcaac tccggacccc ggagttcttg
     3601 aggttcttta atacagggta gattagatat cgcgttgact acaccatatt tatgctttac
     3661 tgccgtatag aaggccttag cgagctcgag ggatcgattc gctgtttctc gagctatctc
     3721 aagccttgtc acattcatta caggaacagc aagcccaaaa tgggttgtag acagcgcccg
     3781 aagtcgctct tttatcgctt catgcgcaag cctggccaag gatggatcga gctccgagag
     3841 ttcatcgagc caatatgttt cggtttttgg gctagttgca gaacgcaata cagattcacc
     3901 gtttcgaacc gccacaaagc tttttgcaga ttgccattgc gatctcgctt cggcaataat
     3961 cgaccaaatt tcataattct catatttttc agaccaggat gaataaccat caaaggcacc
     4021 atcagccata tcctggccta taacaatctc tcccctatca ggatgggtct tgatgtattc
     4081 ccatggtcgt tcaaaaccaa caaatggcaa gcgcgcgata ctcgagataa gcgaataaag
     4141 ccctccaaat gatggcacaa agcgacgaga aaaacctggc aaaaagcctg cccaaaagct
     4201 atcgtctgca tcgatatcga gcaccactag catatcgaca ggctgtgcta tgcctccaga
     4261 atctccttca atgcccatct cgatatctct ctcaggatcg ccctcagcat ctaggccgtg
     4321 cgcacaacga aaagtttcat gcaactgctc agctcgaatt gctttgagca tgcgggcggc
     4381 gctgaaataa tactcagcca aatcgccctg attgatcgca ggaataagcc ttatcgatgc
     4441 tcctgttgta gggtttttga gcgtgagtgg attgtaccgc tttcttgcct ccaattcaga
     4501 aataaacgaa cctatcgcat tgaaaggaat tgcgctgtaa tataaagata tagcttcgat
     4561 ccccacacta cgatagatgt cgatgtgcga tcccgtaaac atacattcct gcggccgtgc
     4621 aatcggcaca tatgaaccaa aggtctgaag cactcctgaa ccatcgggcg cattaattgc
     4681 ccatgaaaga gcgtacctta gctcctcagg tgtgtgagcg gtaagaagcc cattgttcca
     4741 ggacataagt tcgatttcgt cgaccccagc aataaccctc atcttaatcc gctctattat
     4801 gtctggagca aagctcggca gaatatgtcc caaggtgaac gcgttatcga aatcccatgc
     4861 gcacttgacc tcgattccat ctttggaaag ctcatcgagg ctatcaagaa tggagcgaat
     4921 aacgcgaatg tccttgccaa acccgcgctc atcaggcgta tctccccgat aagaatgata
     4981 gaaattcacg tgaaaccgag gacaaaaaaa gatttttgtg ttgctgccta ccatttttct
     5041 ttagcaaaat ctccattagc cggttttgaa gcttttatct ttctgtgtcc tatatatgcc
     5101 tctttccggc cttctttcaa tatagcgttt caagcttttt ttcactaccg ctgttcgctc
     5161 tgactgtcct cttgagagaa agatgccctg cattattctc taataaagct ataagttaat
     5221 gatatctttt gtataatttc gcaggaacgc aggtgctaca cttttttacc aatgtgctca
     5281 tggtaatgag ccttataa
//
LOCUS       DAOMZL010000098         5287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_14648_length_5287_cov_15.583333, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000098 DAOMZL010000000
VERSION     DAOMZL010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14648_length_5287_cov_15.583333"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2125)
                     /locus_tag="PLQ30_07190"
     CDS             complement(<1..2125)
                     /locus_tag="PLQ30_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02268.1"
                     /translation="MQILVCPIFVTIEQTCYTRYMSKKQSAVMEAATRFTPEVQDELR
                     SIVLAMEGREVFAVGMLNSKGLVENLEILGRGTEGAVPAPFQRRSYAQVLIHNHPSGI
                     LFPSDADVVVAAEASAEGFGSYIVDNEVSHVLVVAEPIKPKLIRSLDADEIAAVLDSH
                     GKLSHIMPEFEARLSQVEMAHDVTEIISDGGILVAEAGTGVGKSFAYLIPALAWAIGN
                     SEPVVISTATINLQQQIYKKDFPIVSSLFKKPAKAVVVKGRGNYLCKRRLYAAIEEDA
                     LFSDSSMKLREILEWDNGGGSGDKSDLALPDDDPIWSRVCSESDYCLSLHCPYHDKCH
                     VIHIRLEAASAQLIIANHHVLLADLEAKRIREGIINTVLPSYQALVIDEAHALEASAT
                     SLFSETFSKRSIQRLLLRLSRHKKRIQVGILASLSKLPDIPSMLIDAARLQLENTESS
                     ADAFNAVAATCFLENESSVLVKNLSGLNRTMFLSALQNLEKEIALLVTRLGEISEAIA
                     QELEDEESVIELRITLRSLEEMAALLAQFIKPESEPGSIFWLQVDKKNPKEPIVICSA
                     TPLDVAPILSERLFEKIRSCICTSATLTINGSFQWWRSRVGLLGTGISGDRLSIVELS
                     DKKLKHRQSIPTLEEDSTKNLRVLQRNYPSPFPYSKNAMLAIDTEAPSPDSAEFQTYL
                     NTAVAKLLKASRGRALVLFTSHKALR"
     gene            2209..2511
                     /locus_tag="PLQ30_07195"
     CDS             2209..2511
                     /locus_tag="PLQ30_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02269.1"
                     /translation="MDSKTEQNIGLSLGKTHYVQPRWRSMLAEDIYRHYKNEEKDSYV
                     RALAKLQEEDEEILLPPERGPIVFRDGVFQIDMSRIEEKGDIDLELKKLIDTIMKP"
     assembly_gap    2575..2674
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2760..3542
                     /locus_tag="PLQ30_07200"
     CDS             2760..3542
                     /locus_tag="PLQ30_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPW02270.1"
                     /translation="MIKSFSWNVNGLRACAEKGFIDWVEMTAADFICIQEIKLQEPQL
                     TDELRHPMAFNGKRYDSYWAFSEKKGYSGTGLYVLREPVSVTGLGISEFDSEGRTVIA
                     DYGGFLIISAYFPNSQEAGMRLDYKLRFCDAILELCNFERSKGKHVIVAGDFNIAHKP
                     IDLARPEQNEGNAGYLPEERAWMDKFISIGYIDTFRWFCKEPGRYTWWSYRSPKAREK
                     NIGWRLDYHCVDPEFSSALLAADIHAEIMGSDHCPVSLFLDL"
     gene            3616..4170
                     /locus_tag="PLQ30_07205"
     CDS             3616..4170
                     /locus_tag="PLQ30_07205"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPW02271.1"
                     /translation="MRVRYNAPVTLTFTLCAAIVLILSQTIMPAILPSIFSTPTPFRS
                     NEFMDYIKLFTHVLGHANIQHFISNFTMILLLGPILESAYGSGFLLISILITALVTGL
                     SNAFLFPNVVLLGASGVVFMMIMLSSITNFSKGEVPLTFILIMIIYLGGQIWDALTKQ
                     DNISQFSHIIGGLVGSFLGFYHKK"
     gene            4201..>5287
                     /locus_tag="PLQ30_07210"
     CDS             4201..>5287
                     /locus_tag="PLQ30_07210"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPW02272.1"
                     /translation="MYGKIRDELTKDLEELHTEGTYKDERVIVTPQGATIRVSDGAEV
                     LNFCANNYLGLANDPSIREAAVKAMEEFGYGLSSVRFICGTQVQHKKLERRIADFLGM
                     EDSILFSSCFDANGAVFEPLLDEESAIITDSLNHASIIDGVRLCKAKRWIYKHADMRD
                     IEERDPDTGKPLKGLERCLKEAQGSHRIMIATDGVFSMDGDIANLNAICDLAEKYGAL
                     VMVDDSHATGFMGATGRGTWEQCRVGGRVDIITTTFGKALGGASGGVIASCKEIVEYM
                     RQKGRPYLFSNTLAPAIVGGTLRALDLITSSSELRDRLDFNTKLFRSLMTEAGFDIRE
                     GEHPICPVMLYDEKLAHRMADELLSEGI"
BASE COUNT         1354 a         1139 c         1264 g         1430 t
ORIGIN      
        1 cgcgaagtgc cttatgactt gtaaaaagca ccagtgcgcg tccacgtgaa gccttgagca
       61 gtttcgcgac cgctgtgttt aagtaagtct ggaattcagc gctgtctggc gaaggagctt
      121 cggtgtcgat ggcgagcata gcattcttgg aataggggaa tggcgaagga taatttcttt
      181 gcagaactcg gaggtttttc gtagagtctt cttctaaggt cggaattgat tgtcgatgct
      241 tcaacttttt gtccgatagt tcgactatcg acaatctatc ccctgatatt ccagttccca
      301 ataatcctac tcggctccgc caccattgaa aggagccgtt gatcgtgaga gttgcgcttg
      361 tgcaaatgca gctacgaatc ttttcaaaaa gtcgttccga gagtatggga gccacatcta
      421 gaggagtagc gctgcatatc acaataggct ccttaggatt ctttttatcg acttgaagcc
      481 agaatatgga accgggttct gattctggtt ttatgaattg agccaataga gctgccatct
      541 cttccagaga tcgaagtgtg atcctgagtt ctataacact ttcttcgtct tccagttcct
      601 gggcgattgc ttcagagatt tcgccaagtc gcgttactag caaggcgatc tctttttcga
      661 ggttctgcag agctgacaga aacatggtac gattaaggcc agacaaattc tttacaagaa
      721 cgcttgattc attttccaaa aagcaagttg cagcgacagc attgaaagca tcggctgatg
      781 attcggtatt ctctaattga agacgagcag catcgatcag catcgatgga atatcgggca
      841 atttcgatag cgaagccaat attcccacct ggattcgctt cttatgtctt gaaagtctaa
      901 ggagcaatct ttggatagac ctttttgaaa aagtctcgga aaagagactc gttgcacttg
      961 cttcgagtgc gtgagcttca tctatcacaa gtgcctgata cgaaggcaaa accgtattga
     1021 tgatcccttc tcgtattcgc tttgcttcta agtccgctag taaaacatga tgattggcta
     1081 tgatgagctg tgcgcttgcc gcttccagcc ttatatggat tacatggcat ttgtcgtgat
     1141 aaggacaatg cagggataga caataatcgc tttcggagca tacgcgactc catataggat
     1201 catcatccgg cagagcaagg tctgacttat ctccgcttcc tccaccgttg tcccattcga
     1261 gaatctctct gagtttcatc gaagaatcgg aaaagagagc atcttcctct attgccgcat
     1321 agagtcggcg cttgcataga taattccctc gccccttgac aacaactgcc tttgcgggtt
     1381 tcttgaacaa ggagcttact atcggaaaat cctttttata gatttgttgt tgaagattga
     1441 tagtagccgt agagatcaca acaggttcgc tgtttccgat tgcccaggct aaggccggta
     1501 taagatatgc aaaggatttt cctacgccag tacccgcttc ggcaacaaga atacctccat
     1561 ctgagataat ctccgttaca tcgtgagcca tctctacctg tgagagccgc gcttcgaatt
     1621 cgggcatgat atgtgacagt tttccatgag aatcgagcac ggctgcgatt tcgtctgcat
     1681 ccagggatcg gatgagtttg ggttttatcg gttcagcgac aacgagcaca tgtgagactt
     1741 cgttgtcaac aatatagctg ccaaagcctt cggcgcttgc ctcggccgct accacgacgt
     1801 ctgcgtccga agggaatagt attccagagg gatggttgtg gataagcacc tgtgcatatg
     1861 aacgtctttg aaagggcgcg ggtactgctc cctcagtgcc acgacctaag atctctagat
     1921 tctctacaag acctttagaa ttgagcatgc ctaccgcgaa tacctctcta ccttccatgg
     1981 cgagaactat agacctaagc tcatcctgaa cttctggagt aaaacgcgtg gccgcttcca
     2041 tcaccgcaga ctgctttttg ctcatatagc gagtatagca tgtctgttcg attgttacga
     2101 atattggaca aaccaggatc tgcacttatc gtaaatccgg atggaaggtt cgatgctgtg
     2161 ccaggtagtt gcgaagacta agactccgag tatatcctat agactcagat ggattccaaa
     2221 actgaacaga acatcggctt aagccttggc aagacccatt atgtgcagcc tcgctggcga
     2281 tctatgcttg cagaggatat ataccgccat tataagaatg aggaaaaaga cagctatgtt
     2341 cgcgctttgg cgaaacttca agaagaggat gaagagattc tcttgccgcc cgagcgagga
     2401 cccatagtct tccgcgatgg agtatttcag atcgacatga gccgcataga agagaagggc
     2461 gatatagatc tcgagctgaa aaaattaatc gatactatta tgaaaccttg aagagggcat
     2521 gttaggaatg tgtctttgat gtcaatgcag ggcgaaatgc gagcgaaatg cagannnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcgaaa tacaaggcga aatgcgcaaa
     2701 aagctctcca tgcggccaat gcagcatata agacaaaaga caactgaggg acaaggttta
     2761 tgatcaagag cttttcatgg aatgtgaatg gtctccgcgc ctgcgctgaa aagggcttta
     2821 tagactgggt agagatgact gctgcggatt ttatctgtat acaggagatt aaattacagg
     2881 agccgcaact tactgatgag cttcgccatc ccatggcatt caatgggaag cgatatgata
     2941 gttactgggc tttctcggag aagaagggtt attctggaac aggtctctat gtgcttcgcg
     3001 agccggtttc cgtgaccggc ctgggtatct ccgaattcga ttctgagggc agaactgtca
     3061 ttgccgatta cggtggattt ctcattataa gtgcttattt ccccaacagt caggaagcag
     3121 gcatgcggct cgactataag ctccgtttct gtgacgcaat ccttgagcta tgcaatttcg
     3181 agcgttcaaa aggcaagcat gttatagttg cgggtgattt taatatagcc cataaaccta
     3241 tcgatctggc ccgacctgag caaaacgaag gcaatgctgg atatttgccc gaagagcgtg
     3301 cctggatgga caagttcatc tctataggct acatcgatac attccgatgg ttctgcaagg
     3361 agccggggag atatacctgg tggagttatc gctccccaaa ggctagagag aagaatatcg
     3421 ggtggcgcct ggattaccac tgtgtcgatc cggaattctc gtcagctttg cttgcggctg
     3481 atatacatgc cgaaatcatg ggatccgacc attgcccagt gtctcttttt ctcgatctgt
     3541 gatagcatcg aattaaataa aaaatgaccg gctcgaagtc ggcttcctgt tttttgttct
     3601 tctggagggt ttggcatgcg tgttcgatac aatgcgccgg ttacgcttac ctttacttta
     3661 tgtgcagcta ttgtgctcat attgagccag actattatgc ctgcgattct cccttcgatt
     3721 ttttctaccc ccacaccatt tagaagcaat gaattcatgg attatatcaa gcttttcacg
     3781 catgtacttg ggcatgcgaa tattcaacac tttattagca actttacgat gatcttgctt
     3841 ctgggaccta ttttggagag cgcatatggt tcgggttttt tgctaataag catactcatc
     3901 acagcgcttg taacagggct ttcaaatgct tttcttttcc cgaatgtggt gcttcttggc
     3961 gcttcaggag tggtgttcat gatgatcatg ctttcgtcta tcaccaattt ctcaaaaggt
     4021 gaggtgccac ttacattcat tctcatcatg atcatatatt tgggcggaca gatttgggat
     4081 gctcttacaa aacaggacaa tatttctcag ttttcccata taataggagg ccttgtcggc
     4141 agcttccttg gtttctatca taagaagtga atacaagaaa atcgaatatg aggtgtcatt
     4201 atgtacggta aaataagaga tgaacttacg aaggatctcg aagagcttca tacagaaggt
     4261 acctacaaag atgaacgtgt catcgttacg ccgcaaggcg caactattcg tgtttcagat
     4321 ggtgcggaag tgctgaattt ttgcgcgaat aactaccttg gtcttgcgaa tgacccgagc
     4381 atccgcgagg ctgctgtgaa ggctatggaa gaatttggct atggactctc ttctgttcgt
     4441 tttatctgtg gaacgcaggt acaacacaaa aaactcgagc ggagaatagc agattttctt
     4501 ggcatggaag attcaattct cttttcatct tgcttcgatg cgaatggagc cgtttttgaa
     4561 cctcttcttg atgaggaatc ggcaatcata accgatagcc taaatcatgc ttccataatc
     4621 gatggtgtgc gtctatgtaa ggcaaaacgc tggatataca agcatgccga tatgagagat
     4681 atcgaagaaa gagatcctga tacagggaaa cctctaaaag ggctcgaacg ctgtctaaag
     4741 gaagcccaag gcagccatag gataatgatc gctaccgatg gcgtgttctc catggatggc
     4801 gacattgcca atctgaatgc aatttgcgat ttggcagaga aatatggtgc gctcgtgatg
     4861 gtggatgatt cgcatgcgac tggtttcatg ggggcaactg gacgtggcac ctgggaacaa
     4921 tgtcgagtcg gtggtagggt agatatcatt actacgacct tcgggaaagc acttggagga
     4981 gcctcgggcg gagttattgc ttcctgtaag gaaattgtcg aatacatgcg acagaaaggg
     5041 cgtccatatc ttttctcgaa caccctggcc ccggccattg taggtggtac tctgcgggca
     5101 ctcgatctta taactagttc ttcagagctg cgagatcgcc tcgattttaa taccaagcta
     5161 tttcgtagcc ttatgacaga agcaggtttc gatataagag aaggcgaaca cccaatctgc
     5221 cccgtaatgc tgtacgacga aaaacttgct catagaatgg ccgatgaact tctttccgaa
     5281 ggcattt
//
LOCUS       DAOMZL010000099         5278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_14685_length_5278_cov_10.451656, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000099 DAOMZL010000000
VERSION     DAOMZL010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14685_length_5278_cov_10.451656"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PLQ30_07215"
     CDS             complement(<1..702)
                     /locus_tag="PLQ30_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02273.1"
                     /translation="MAIERQSLVRRHNPVLQKYDPFSPLSVGNGEFTFTADITGLQSL
                     ISDIPGITPLCTMSQWGFHSYPEREDCPRDEKRLRLSYFDPIRNTGGYMIDPTGQEEL
                     FNELRINPHRFNLARVGFVFEGEKLEARDISEIEQVLDLWKGELHSRFSYKDQPVDVF
                     TICDPEVDALAIRVFSPLLDQGRLCVRIAFPYGSHEIDASDWIAEQRHSTIVLEHRKT
                     SKSEKGESLSLERILD"
     gene            complement(706..1599)
                     /locus_tag="PLQ30_07220"
     CDS             complement(706..1599)
                     /locus_tag="PLQ30_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HPW02274.1"
                     /translation="MKRENIQIRDPFVLPVKDEMRYYLYGTTDSDPWKAPGIGFDAYI
                     SENLEEWEGPYPVFRPQSDFWASHNFWAPEVYKYGKSYYMFASFKSSSHERATQILRA
                     ASPFGPFVQHSNEPVTPASWECLDGTLWEEEGQPWLVFSREWVQVNDGEVWAAPLSID
                     LDRLDGEPILLFRGSDASWTRKIKRRNGSGFEDARVTDGPFLYKTDQGSLFMLWSSIG
                     ESGYAMGYARSESGKLIGPWLQQSKPLVDGGRGHGMIFSGFNGRTYLAYHFPNETPYE
                     RFCYREIEVLSTSIAFIGEEL"
     gene            2076..3002
                     /locus_tag="PLQ30_07225"
     CDS             2076..3002
                     /locus_tag="PLQ30_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPW02275.1"
                     /translation="MNERARKKAIRENLTSYAFLVPWLLGFFVLTLYPMIYSFFLSFT
                     EFNILQPPKWLGLRNYFVMFIGNEQFPRDERFLNSLLVTFKYVFISVPLKLIFALGIA
                     MLMNRDLKLIPFYRTLYYIPTLLGGSVAIAALWRRLFAGDGVLNMILRSAFNLNPPAW
                     ISNPKYALYTLILLSVWQFGSPMIIFLAGLKQIPKEYYEASAVDGAGKMRQFFSITLP
                     CLSPIIFFNLVMQMINAFQSFTQAYIISGGQGGPLDSTMFYSLYLYLKGFAFSEMGYA
                     SAMAWVLLIIIAALTALAFKVSGSLVSYGSGE"
     gene            2999..3847
                     /locus_tag="PLQ30_07230"
     CDS             2999..3847
                     /locus_tag="PLQ30_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPW02276.1"
                     /translation="MKRRTTSLIKSILTNILILLLGMCMLYPVVWLIAASFKESNTIF
                     SDPGLWPKAFTLQNYVKGWQGIGIVGFATFFKNSFIISGLCVIANVIFCSLTAYAFAR
                     LKFAGRNIWFAIMMVTMMLPTHVTTIPRYIMFRKFGWIDTFLPLVIPKFFATDAFFIF
                     LLVQFIRSLPKELDESALIDGCGKFGIYTRIVMPLTKPALITTVIFTFLWTWDDFFNQ
                     LLYLNSPSKYTVPMGLRLFLDSSGISSWGPMFAMSVLSLLPCLILFFSLQKYFVQGIA
                     TTGIKG"
     gene            3879..5135
                     /locus_tag="PLQ30_07235"
     CDS             3879..5135
                     /locus_tag="PLQ30_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPW02277.1"
                     /translation="MKRTSLALLIILMSAAMVFGQQQITIRWAYWGSESRVKRSQEAI
                     NLFEAANPGIKVNPEISGGTGDHFNKVDTQLAGGSGPDIIQMGGNINDYVKREVLLPL
                     EKYAGTILNTAVIDPSAIESGTIDGHLYGVSTGVTMPALVYNKSLIQRVGAPLPKVSM
                     TYEEFRNYLVMLKSKLPSGTYPMQDIGALSSNSTPFGYWTRYNGTPLYNAATKTTAVR
                     PADAQKYLELFKDYRNNGLIPPADIAAGYAETNADTSMVIAGKAAIGFIWTNQLAGYQ
                     AATTDELCLMEFPGAAATKALWQAPSQFYTVNKNSKHPEEAVKFINFLVNSPEAAKVL
                     GNDRGASASATARAAGAADINDRKVLEYLDVAGPHSSKETDHVPNDTEFNSTLFLIYQ
                     KVAFGQISTAAGGQQIYDLIVRLIKK"
BASE COUNT         1432 a         1224 c         1118 g         1504 t
ORIGIN      
        1 atctagaatc ctttccagcg aaagcgattc tcccttctct gatttggacg tcttcctatg
       61 ttctaaaacg atagtgctat ggcgctgttc tgcaatccaa tcagaagcat caatttcgtg
      121 cgaaccatag ggaaaagcaa tacgcacaca aagacgaccc tggtcaagaa gtggagaaaa
      181 gacacggata gctaacgcat ccacttcagg atcacagata gtaaagacat ctacgggctg
      241 atccttataa gagaatcggc tatgcagctc acctttccat agatcgagaa cctgctcgat
      301 ttctgatata tcccgagctt ccagcttttc cccctcaaag acaaaaccga ctctagcaag
      361 gttaaacctg tgtggattga ttcgaagctc attaaataac tcttcctgcc ctgttggatc
      421 gatcatatag ccgccagtat ttctaattgg atcgaagtag ctcagcctga gccttttttc
      481 gtcgcgaggg caatcttcgc gttccggata agaatggaat ccccattgcg acatcgtaca
      541 aagtggcgta attccaggta tatcggaaat aagactctgc aagccggtga tatccgcagt
      601 aaaggtgaat tcgccgttgc caaccgatag aggagagaat ggatcatact tttgaagaac
      661 tggattatgt ctacgaacaa gagattggcg ctctatagcc atgatctata actcctcgcc
      721 aataaaagcg atacttgtac tcaatacttc tatctcccga taacaaaagc gctcataggg
      781 agtttcattg gggaaatggt aagccaggta tgttcttcca ttaaagccag aaaaaatcat
      841 gccatggcct cgccctccat cgacaagggg ctttgattgc tgaagccacg gtccaatgag
      901 ctttcctgat tcagagcgag catatcccat tgcatagccc gattctccaa tactagacca
      961 gagcatgaat aacgatccct ggtctgtttt gtaaagaaag ggtccatcgg tcactcgagc
     1021 atcttcaaag cctgagccat ttcgtctttt aattttcctt gtccaggacg catccgaacc
     1081 tcgaaaaagc aagatgggtt cgccatccag ccgatctaga tcaatcgaaa gaggagctgc
     1141 ccaaacttca ccatcattta cctggaccca ctccctagaa aagaccagcc aaggctgacc
     1201 ctcttcttcc cagagagtgc catctaagca ttcccagctc gctggagtca ccggctcatt
     1261 tgaatgttgt acaaatggac caaaaggaga agctgcgcgg agaatttgcg ttgcccgctc
     1321 atggctagat gacttgaaac ttgcaaacat atagtaggat tttccatact tgtagacttc
     1381 cggcgcccag aaattatggc ttgcccaaaa atcagactga ggcctaaaaa caggatatgg
     1441 cccctcccat tcttcgagat tttcgcttat atatgcatcg aagccaatgc ctggagcttt
     1501 ccaggggtcg ctatcagtag tcccatagag gtaatatcgc atctcatctt ttactggcaa
     1561 cacaaatgga tcgcgaattt ggatattctc tctcttcatg gctttctctc ttatatctat
     1621 gcgcttgaag tcttaaatcc tattgaatat tagcataata acagatatgt ttttctagag
     1681 caattccgtt agaaatgcca atctgctggt ctttttctct tattatgagc ctgccggagc
     1741 gatacagaaa agctctttgc ctatgcaaga aataattata ccctcatcta aaagcaattt
     1801 ggaaattcat gctcatcaaa aaagatcatg accggaatat acatatattt atatatatat
     1861 atgatgctgc ttctgaaatc aaaggggtat gaaaaaagtc aataatagat atctattgtc
     1921 aatattttac atggcatttt gcacaaaagg atcctaataa tagaaatgat aaatatgctt
     1981 ttgtcgttga tgaggtcaat cggttttcag gagcatcttt gcactacaga tgatgtatga
     2041 gggattaagc cataaatcca gagaagagaa agaaaatgaa tgagagagcc aggaaaaagg
     2101 ccatccggga aaacctgaca tcgtatgctt tcctagttcc atggttactt ggcttttttg
     2161 tgctaactct ttacccaatg atttattctt tctttctatc ctttaccgaa ttcaatatcc
     2221 ttcagcctcc caaatggctt ggcctgcgca attactttgt aatgttcatt ggaaatgaac
     2281 aattccccag ggatgaacga tttctgaatt ctctcctcgt tacgttcaaa tatgtgttca
     2341 tctctgttcc tcttaaacta atatttgcgt tgggcatagc gatgttgatg aatcgagacc
     2401 tcaaactcat ccccttctat cgaactctct actatattcc cacccttctg ggtggttctg
     2461 tcgccattgc tgccttatgg agaaggctct ttgctggaga tggcgtattg aatatgattc
     2521 tccgcagcgc tttcaacctc aatccgccag catggatatc gaatcctaag tatgccctct
     2581 ataccttgat tctgctatcg gtgtggcaat ttggatctcc tatgataatt tttcttgcag
     2641 gtcttaagca aataccaaaa gaatactatg aggcttcggc ggtagatgga gctggcaaga
     2701 tgcggcaatt cttctctatt accctaccct gcctatcgcc aatcattttt tttaatcttg
     2761 ttatgcagat gatcaatgct ttccagtctt ttacacaagc ttatatcatc tcaggtggac
     2821 aaggcggtcc gctagactcc acgatgttct acagcctcta tctttactta aagggttttg
     2881 cattctctga gatggggtac gcgtcagcta tggcctgggt tcttcttatc ataatcgcgg
     2941 ctcttaccgc gcttgccttc aaggtatcag gctcactggt cagctatggg agcggagaat
     3001 gaaaaggcgc actacgagct tgataaagtc tattcttaca aatatcctca tcctcttgct
     3061 cggtatgtgc atgttgtatc cagtcgtatg gctaatcgct gcatcattca aggagagcaa
     3121 tactatcttc agcgatcctg ggctatggcc taaggccttc actttgcaga actatgtcaa
     3181 gggctggcaa ggaattggta tagtagggtt tgccactttc tttaagaatt ccttcatcat
     3241 atccgggtta tgcgtaattg caaatgtgat attctgttcc ctcactgcct atgcatttgc
     3301 gaggcttaag tttgcgggaa gaaatatttg gtttgccatc atgatggtta ctatgatgtt
     3361 gccaacccac gtgacaacaa tcccacgata tatcatgttt aggaaatttg gatggataga
     3421 tactttcctt ccactggtca ttcccaaatt cttcgctact gatgcatttt tcattttttt
     3481 gcttgtgcaa ttcattcgca gtcttcccaa agaactagat gaatcggctt taatcgatgg
     3541 ttgtggcaaa tttggaatct atacacggat tgttatgcca ctaaccaaac cagctctgat
     3601 aacaacagtg attttcacct ttctgtggac atgggacgac ttctttaatc agctgcttta
     3661 ccttaattct ccgtctaaat acacagtccc catggggctc aggctcttcc ttgattcctc
     3721 tggtatttca tcttgggggc ctatgttcgc catgtcggtt ctttctctgc ttccttgcct
     3781 catcctcttt ttctcgctcc agaagtactt tgtgcaggga atagctacga cgggcattaa
     3841 gggataagta tttttattct ttcgatagga ggtagatcat gaaaagaacg agtttggcac
     3901 tgctcattat tttaatgagc gctgccatgg tattcggaca acaacagatc acaattcgtt
     3961 gggcttactg gggaagcgaa tcccgagtaa agcgaagtca ggaagccata aacctcttcg
     4021 aggcagctaa tccgggcatc aaagttaatc cagaaatctc aggtggaaca ggagatcact
     4081 tcaataaggt cgatacgcag ctagctggcg ggagtggccc tgacataatc cagatgggtg
     4141 gcaacataaa tgattatgtc aaaagagaag tgctccttcc cctggaaaaa tacgctggga
     4201 ctattctcaa cacggcggtg atcgatccaa gtgcaataga atctggcact atcgatggac
     4261 atctctatgg tgtttccacg ggtgtaacaa tgccggcgct tgtctacaat aaatcgctta
     4321 tacaaagggt tggtgcgcct ctgccaaagg tctcaatgac ttatgaggaa tttcgcaatt
     4381 atcttgtgat gctcaaatca aaacttccgt cgggaacata tccaatgcag gatatcgggg
     4441 ctctttcgtc caactcgact ccttttggat attggactcg ctacaatggc actcctctct
     4501 acaatgctgc gaccaagact accgccgtaa gaccagcgga tgcacaaaag tatctcgagc
     4561 tctttaagga ttaccgcaac aatgggctca ttcctccggc agatatcgcc gcgggttatg
     4621 cagagacaaa tgccgacaca tcgatggtca ttgccggtaa ggctgccata ggatttattt
     4681 ggacgaacca gcttgcaggt tatcaggcag ccacaaccga tgaactgtgc ctgatggaat
     4741 tccctggtgc ggcggctact aaggcgctct ggcaggctcc gagccaattt tacacggtta
     4801 acaagaattc taagcatcca gaggaagccg taaagttcat taacttcctt gttaatagcc
     4861 cagaagcggc aaaagtactt ggaaatgatc gtggtgcctc tgcctcggct accgctagag
     4921 ctgcgggagc agccgacatc aatgatagaa aggttctaga atacctggac gttgcagggc
     4981 cacattcttc aaaagaaacg gatcatgtgc ccaacgatac ggaattcaat agcaccttgt
     5041 tccttatcta tcaaaaggtg gcatttggcc agatatctac agccgcgggc ggacaacaaa
     5101 tctatgatct tatagtaaga ctcataaaga agtgaaccat gattgatgaa gcccataaag
     5161 aaaatgcttg ttctatctcg aagggccata tttgagcagt tctaagaaag gggcgcggct
     5221 ttggatatgc tgcacccctt cttgcttctt ctataattag ttcactcttt ggattgcc
//
LOCUS       DAOMZL010000100         5043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_15625_length_5043_cov_13.516239, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000100 DAOMZL010000000
VERSION     DAOMZL010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15625_length_5043_cov_13.516239"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1417)
                     /locus_tag="PLQ30_07240"
     CDS             complement(8..1417)
                     /locus_tag="PLQ30_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW02278.1"
                     /translation="MKFADFKYGLSEFWSEFKKERSGLVGLAILILSILVVIFEPLIL
                     PWKEANTKWRSIDYWQDNSAGAPPAWTNFFSKQKAAITVRLENPKDEVIEMDGGIKLA
                     TYTFDYDYTADKAPLDVIFHMIGHGDFPVQVAVERPDGQIIELAQRFEQGLSGQDIRI
                     SLDNDGRESVFTFIKSHESEEALKEYSSKSLRTCNIVFNQTREGMATEFKSLKGNYKF
                     IVRALLLVPGYSEVEDPYTVVTGSVSGLLGTDDSKRDIFSGLVAGLKWALIIGLLTSA
                     ISVLIGVMYGIVSAYFGGAVDSVMQFIYQIVNSVPILPVLIVVSAIFKPSIYMLITAM
                     VLFFWTGSVMTVRSMALQIKEETYIEAAKALGAKHSRIIFKHMVPLLIPYSFASMALS
                     VPSAIVYESSVSLLGLGDATIVTWGQILHDAMQGAAILKGIWWWVLPPGLLIAIMGMT
                     FAFLGFSMDKILHPKLKTR"
     gene            complement(1414..2454)
                     /locus_tag="PLQ30_07245"
     CDS             complement(1414..2454)
                     /locus_tag="PLQ30_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW02279.1"
                     /translation="MYKWFAVKRMLKGIFTYVIIIFIMSVLFNTVNEQTMRSSIEEQV
                     RAETMRLKNMQPSALQRFQEQRRQELIRLYRLDRPLMERILFRTWNTITFNFGKSTII
                     KSSRGDREVLTIVGEAIPRSLILFTVAMAIEIVVGIILGLKKAQKPGGSLDRSTSLIT
                     MIVYGMPTFWLAMILIMFFVYQLRIFPSGGMHTVPPPQGIMYYIDFLWHMTLPLLTLL
                     LIGFWGIAFVVRNIVLSILQEDYIMAARARGIPENKVLFGHTLRTAAPPLITMSVLSL
                     LASIGGAIIFEGIFSWPGLGNLYWIAVQQNDIPVLMGDLAITVGLYQVGLILLDLTYG
                     FMDPRIKVGGKA"
     gene            complement(2578..>5043)
                     /locus_tag="PLQ30_07250"
     CDS             complement(2578..>5043)
                     /locus_tag="PLQ30_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW02280.1"
                     /translation="MDQTIAIKDTAEGKTDVFFTGLDGKTYNGILPSDLEKLSTYAVP
                     SGSWSLLLNNIPNKAPYTFQTKDGRTIFNPLAIQEVRYALNWLIDRKKIVDEILMGAG
                     EPMITAQTPGQPGTYRFNLVPAKLGMTVRGDEKRALSDIDAALRAAANLPENKGKLVK
                     SGQWWTYNKEPITVKFIIRVDDPTGRLLEGRYIADQIEKAGIKVERLEYDRSKASNLA
                     YSSDPADWEWSMYTEGWGAGATRAWWDVTISQMYSPYYGYMPGGMTEGFWNYENKEID
                     RIAQKNINGWFLTADEYWSDNIKVQEMALKEAVRIYVCSQIQYYVANKDRFNSRMLYG
                     LGDGLNNWSVRSADVKPNEKNEKILKITQFSARGALFMSAWDPVGVDGFSDVYSAAII
                     EACTDPSTFESPNAAKDTPLRVKYDLKNVETKIVAGQPGEPPVGQIPVDKAAVIFNSK
                     TKKWESGIEYKDVTGEGTYDYVKNNDLKSYSKLANQQYIYGKWHSGQPITVADIMYAT
                     AFAYEWANKDGEDDKYYDAAYAAQYQAYLPVSKGTVLNKDGSFTTYFDFNWPMDKDRV
                     AATGAVSPKAGNPGRQTLVSWEIYEALAKLVAEGSKSGTQYSFSNDPSMTEVDIINLK
                     CVEDIKAKLQDFVAAKYVPDCIKQWVTPEQAVARYNAAIKFIDTYGNAYISNGPFVLS
                     KIDFNTNYVELSAFRDYTYKADYFPKLFRTTITRIDEVKVPATAARNVDAKIDLQISA
                     VTYPDDTAKPADNKAKVTVTLIKADNTETVYTAKYVKAGSFQAVIPAKDLAALKPGSY
                     TIVVQSVFTTEAPAVQAASLVVF"
BASE COUNT         1298 a         1246 c         1157 g         1342 t
ORIGIN      
        1 atcgaggtta ccttgtcttt agttttggat ggagtatctt gtccatcgag aagcccagga
       61 atgcaaaggt catgcccatt atagctatca gtagtcctgg cggcagcacc caccaccaaa
      121 ttcctttgag gatagccgct ccctgcattg catcatggag aatctgaccc caggtgacaa
      181 tagttgcgtc tccgaggcct aaaagactca ccgaagattc atagactatt gcactcggca
      241 cagataaggc catggatgca aagctgtatg gaatcaaaag cggcaccata tgcttaaata
      301 tgatcctgga gtgctttgca cccagcgcct tggcagcctc gatatatgtc tcctccttga
      361 tttggagagc catagatctg actgtcatca cggagcctgt ccagaaaaag agcaccattg
      421 cagtaatcaa catatagatg ctcggcttga aaatagcgga taccactatg agtacaggca
      481 aaattggaac cgaattcaca atctgataga tgaactgcat taccgaatct acggcacctc
      541 caaagtatgc cgagacgata ccatacatga ctccaatcag caccgagata gcagaagtca
      601 aaagcccaat tatcagtgcc catttgagtc cagccacgag accggaaaag atatcgcgct
      661 ttgaatcatc tgttcctaat aatcccgaaa cagagcctgt tactacagta tatgggtcct
      721 cgacctcgga atatccagga acgagcaata aggctcgcac aataaattta tagttccctt
      781 tgagagactt gaattcggtg gccatacctt ctctcgtctg attgaacact atgttgcagg
      841 ttcttagact tttgctggaa tactctttga gagcctcttc gctttcatgg ctcttgatga
      901 aggtgaacac cgattcgcgc ccatcattat ccaaggagat tcggatatcc tggccgctca
      961 atccttgttc gaaacgctga gcaagctcga tgatctgtcc atcgggtcgt tccaccgcaa
     1021 cttgtacagg aaaatcccca tgtccaatca tatgaaatat tacatcgagg ggcgctttgt
     1081 ctgcggtata gtcataatcg aaggtgtagg tggcaagttt tatacctcca tccatctcga
     1141 ttacttcatc cttgggattc tcgagacgga cggttattgc ggctttctgc tttgagaaga
     1201 agtttgtcca cgcgggaggc gcgcccgctg agttatcctg ccagtagtct atactccgcc
     1261 acttagtatt tgcttctttc catggcaaaa ttaaaggctc aaagatcacg actagaatcg
     1321 agagaatcaa tatcgcaagg ccaacaagtc cggaacgctc tttcttgaat tctgaccaga
     1381 actccgatag tccatacttg aaatcagcaa atttcatgcc ttgcctccca ccttgatacg
     1441 cggatccata aacccatagg tgagatccag caatataagc ccgacctggt ataatcccac
     1501 ggtgatggca aggtctccca ttagaacagg gatgtcattt tgctgaaccg ctatccaata
     1561 tagattcccc aaccctggcc aggaaaaaat accttcgaaa atgattgctc ccccaatcga
     1621 cgcaagcaga gacaaaactg acattgtaat tagaggaggc gcggcggtgc gcaaagtgtg
     1681 cccaaacagc actttgttct ctggaatgcc acgcgcgcgt gcggccatga tgtaatcctc
     1741 ctggaggatg ctcagcacaa tattcctcac tacaaaggct atcccccaaa atcctatgag
     1801 aagcaaggtc aagagaggca gtgtcatgtg ccacagaaaa tctatataat acattatccc
     1861 ttgcgggggt ggcacagtat gcatgccccc agaaggaaat atccttagtt ggtagacaaa
     1921 aaacattatg agaatcatcg caagccagaa tgtcggcata ccatagacga tcatggtgat
     1981 gaggcttgtg cttctatcta gcgaaccgcc aggtttttga gcttttttaa gcccaagaat
     2041 aatgccaacc acgatttcaa tggccatagc aacagtaaag agtataagtg aacgcggaat
     2101 tgcttctcca acgatcgtca gtacttctcg gtctccacgc gaagatttaa tgatagtcga
     2161 ctttccaaaa ttgaaagtga tggtattcca ggtgcggaag agaatgcgct ccattagcgg
     2221 acgatccagc ctatagagcc tgatcagctc ctggcgccgc tgttcctgaa aacgctggag
     2281 ggcagatggc tgcatattct tgaggcgcat ggtctcagcc cgcacttgct cttcgatgct
     2341 tgagcgcatt gtctgttcat tcaccgtatt gaaaagcaca gacatgataa aaataattat
     2401 tacataagta aaaatgcctt tcagcatgcg cttgacggca aaccacttat acataagacc
     2461 acctatttat ttttgatccc ttgagagaat tgcacttcga ttattacatt aaaaaagcat
     2521 tttagaaaat aaggagggcg ccgcctcttt tagacagcgc ccttcccctg atttatatca
     2581 gaagactaca agcgaagcag cttgaaccgc aggtgcttct gtagtaaaga cagactgcac
     2641 gacaatagtg taggaacctg gcttcaaagc tgcgagatct ttcgcaggga tcaccgcctg
     2701 gaagctgcct gctttgacat acttcgcggt gtacacagtt tctgtgttgt ccgccttgat
     2761 taaagttacg gtaactttgg ccttgttatc cgctggcttg gcagtatcgt ctggataggt
     2821 cacggcggat atctgaaggt cgatcttggc gtcaacattg cgcgccgcag tcgcaggcac
     2881 cttaacttcg tcgattcgcg ttatggtggt gcggaacagt ttcgggaaat aatcagcttt
     2941 gtatgtataa tccctgaaag cggaaagctc gacatagtta gtattgaaat cgattttgct
     3001 caagacaaat ggaccattgg agatgtaagc attcccatag gtatcgatga attttatggc
     3061 tgcattatag cgagcaacag cctgctccgg ggtaacccac tgcttgatgc agtcaggaac
     3121 atacttggca gcaacgaagt cctgcagctt agccttgatg tcttcgacgc atttcaaatt
     3181 gatgatatcc acttccgtca tagaagggtc atttgagaat gaatactgtg ttccagattt
     3241 agagccttct gcaacgagct tagccaaagc ttcgtagatc tcccaggata ctagagtctg
     3301 gcgtccgggg ttgcctgcct tggggctgac tgcacctgtt gcagctacac gatctttgtc
     3361 catcggccag ttgaaatcga aatatgtcgt aaagctccca tccttattga gcacagtacc
     3421 tttggaaaca ggcaaatagg cctgatattg agctgcataa gctgcatcgt agtacttgtc
     3481 gtcctcgcca tccttgttag cccactcgta ggcaaaggca gtcgcataca ttatatcagc
     3541 taccgttata ggctgaccgc tgtgccactt tccatagatg tactgctgat ttgcaagttt
     3601 gctgtaggac tttagatcat tgttcttcac ataatcgtat gttccttcgc ctgttacatc
     3661 cttatattcg attccgcttt cccacttctt tgtcttagaa ttgaagatta ccgcagcttt
     3721 gtcgacagga atctggccaa caggcggctc gcctggctgg cctgcaacaa tcttggtctc
     3781 gacattcttg agatcgtatt ttacgcgtag cggcgtatcc ttggcggcat tcggagattc
     3841 gaaggtggag ggatcggtac aagcctcgat tatggcagca gagtagacat ccgagaaacc
     3901 atcgacaccc acaggatccc atgcactcat aaatagcgca ccgcgagccg agaactgagt
     3961 gatcttcaat attttttcgt tcttttcatt gggctttaca tccgcagatc gcactgacca
     4021 gttgttgagc ccatcaccta ggccatagag catccgcgaa ttgaaacgat ccttgttggc
     4081 aacatagtac tggatctggc tgcatacata gatgcgtacc gcttccttca aggccatttc
     4141 ttgtaccttg atgttgtcgc tccagtactc atcggcggtg agaaaccatc catttatgtt
     4201 cttctgagca atacgatcga tttccttatt ctcgtagttc cagaagccct cggtcatccc
     4261 gcctggcata tagccatagt agggtgaata catctgactg atggtgacat cccaccaagc
     4321 ccgagttgca ccagcgcccc aaccttcggt gtacatcgac cattcccaat ctgcggggtc
     4381 gctgctgtag gcaagattgc tagccttgga gcgatcatat tcgaggcgct ctaccttgat
     4441 cccggctttc tcgatctggt ccgcaatata tcggccttcg agaagtcgac ctgttggatc
     4501 gtcgacacgg attatgaatt tgacggtaat aggttccttg ttataggtcc accactgacc
     4561 agactttacg agcttgccct tgttttcggg caggttagct gccgctctca atgcggcatc
     4621 tatatctgac agagctcttt tctcatcgcc acgtaccgtc atacctaact tagcaggcac
     4681 gagattgaag cgatatgttc caggctgtcc aggtgtctgg gctgttatca ttggctcgcc
     4741 tgcgcccatc aagatctcat cgacgatctt tttgcggtcg atgagccaat tgagtgcgta
     4801 tcggacttcc tgaattgcta ggggattaaa gatcgtccga ccgtctttgg tctggaatgt
     4861 atagggcgct ttgtttggaa tgttgttcag gagaagagac cagctccccg atggcaccgc
     4921 ataagtagaa agtttttcca gatcagatgg gaggatacca ttataggtct ttccgtcaag
     4981 accagtgaag aagacatccg ttttcccttc tgcggtatcc ttgatggcga tggtctgatc
     5041 cat
//
LOCUS       DAOMZL010000101         4997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_15818_length_4997_cov_10.348847, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000101 DAOMZL010000000
VERSION     DAOMZL010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15818_length_4997_cov_10.348847"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PLQ30_07255"
     CDS             <1..440
                     /locus_tag="PLQ30_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPW02281.1"
                     /translation="ETGRFGFLKDTSCRIGDIQAKIEAAKGSDFKLFNANAATLLASL
                     QKGAAGYSGIMTHFHSDLYVWMCKHWNDRPDRALELQNFLGLASVIEYQLYPINAMYA
                     LQREGLPIKLISRRADARLFTESMRLEVEQLMGLSQRFSEVYR"
     gene            complement(558..1676)
                     /locus_tag="PLQ30_07260"
     CDS             complement(558..1676)
                     /locus_tag="PLQ30_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPW02282.1"
                     /translation="MKKLRVAIIGIGKIGISHLKAIKAIEPLGYAELAAICSLNPNKI
                     KSLCDDYDIPVWYTSYQEMLKSESLDLVHNCTPNVLHYDVNRGILEAGLYALSEKPLT
                     IDSQQSGALMTLAKNRHLKTAVNFVYRYYPIIMHIKELISKGELGTIYSIHGTYLQDW
                     LLFDHDYDWRVESRYGGPSRALADIGSHWIDIAEFLLDRRLESIAADFATFIPKRIRF
                     PSETITVDTEDFASALMRFEGGIHGCLTVSQVSAGRSNGISFEIDGSTASLRWSQEHS
                     ERASLKYREEKELILDEKGIISPANEMEENKEAKAGASRQERFLEVQQRMIDNFYRSI
                     LFSEPPLYADFLQGHHIVQIIEAAIESNQKARWVDIDL"
     gene            complement(1679..2098)
                     /locus_tag="PLQ30_07265"
     CDS             complement(1679..2098)
                     /locus_tag="PLQ30_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02283.1"
                     /translation="MSGIIEYEHEGKGLQRVYENKEWMVGIKNYKPENDISNIDCLER
                     HNETDELFILLRGTCILLCALEDLINDSIELKAISMELNKVYNIPKGLWHNTITQQET
                     KLILIESASTSQQNSNVIPLSYDQIARAKELISALQK"
     gene            complement(2128..3060)
                     /locus_tag="PLQ30_07270"
     CDS             complement(2128..3060)
                     /locus_tag="PLQ30_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HPW02284.1"
                     /translation="MFESKSLRVAYGEALAELGSINQKVVVLDTDLSHATMTSIFASK
                     FPDRFFNFGLAEANMMGAAAGFAHSGLLPFVSTFALFGAGRAYEQIRNSIAYPNANVK
                     FGLSHSGLSVGEDGASHQSIEDIALMRVLPGMTILVPCDPFETRKAVFAAASLDGPVY
                     IRVARPVVEVITNEDSAFAIGKANIMREPGDVCIVATGLMVKEALAAAERLSLNGIKA
                     GVINMHTIKPIDAELILEMAFQCRAMVTAEEHSIIGGLGSAVAEVLVGKSNIKFARVG
                     IQDKFGRSGKPEDLFKAYGLTADDIAQQCIMLLE"
     gene            complement(3053..3871)
                     /locus_tag="PLQ30_07275"
     CDS             complement(3053..3871)
                     /locus_tag="PLQ30_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPW02285.1"
                     /translation="MDELMLEQKALSIRRDIIEMLYRCQSGHPGGSLSCVEILIALYY
                     AVAHVDPTNPSWEMRDRIVLSKGHGCPALYAILADKGFFPRNDLWNLRQIDSHLQGHP
                     DMRKTPGIDVSTGSLGQGASVAVGLALAAKYKRAPYHVYVVLGDGEIQEGMVWEATMS
                     AAHYKLDNLIFILDHNGLQIDGTNDQVMSLGDIIGKFHSFGFECKKVDGHNVDMLVKA
                     ISEPVPGKPLFVLAETVKGRGISFMENNVGWHGKPMNTEEYNQAMCELKGTQNV"
     gene            complement(3888..4763)
                     /locus_tag="PLQ30_07280"
     CDS             complement(3888..4763)
                     /locus_tag="PLQ30_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPW02286.1"
                     /translation="MELQSREVQKSSYKTPRKRKLLNALPGHILLAMMLIFLLFPFYW
                     TFVTSIKPEKELYSSTVTYWPAKVTFGSYKKLFAEYNFIKPMINSLLVAVVTTLLSLV
                     VSLLAAYAFSRYRFKGRQLFMTLFLTNNMFPTVLLLIPLFAIMRKIGILYTPSSLILS
                     YTTFTIPFSVWLLTGYLDDLPRSLEEAAMVDGANRFQAFIKIILPMLLPCIVATGAYI
                     FMTSWNEYTFAVMFTNERNRTIPVALKNFIGQLGVEWGMLTAGGVITIIPVCIMFFFA
                     QKRLVEGLTAGAVKG"
     gene            complement(4763..>4997)
                     /locus_tag="PLQ30_07285"
     CDS             complement(4763..>4997)
                     /locus_tag="PLQ30_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02287.1"
                     /translation="TTLLRIIWVANSVDVIFNLTEGGPAYSTQTLSVYVFNKGNTLNL
                     GYASTLAIFLTIGLSVVSIPYLKNLFKAQEEAK"
BASE COUNT         1388 a         1148 c         1045 g         1416 t
ORIGIN      
        1 tcgagactgg ccgatttggt tttctgaagg atacaagttg tcgcattgga gatatacagg
       61 ccaaaatcga agcggcaaaa ggcagcgact tcaagctttt caatgcaaat gctgcgactc
      121 tgctcgcttc gctgcagaaa ggggccgcag gatactcagg cattatgact cacttccact
      181 cagacctcta tgtatggatg tgtaaacact ggaacgaccg gccagatcga gcgctcgagc
      241 ttcagaattt cctgggtcta gcatccgtca tcgaatatca actatatccc atcaacgcta
      301 tgtatgcact gcagcgtgag ggtttaccca tcaagctcat atcgcgccgc gcggatgcac
      361 ggctcttcac tgaatctatg cgcctcgagg ttgagcagct aatgggatta tcgcaaaggt
      421 tttcggaggt gtataggtga aggtaagggg ttggagctct ggccatgatc gtcaccgcgc
      481 tttctgaact atcgcgctat caaagtgtga ttttaggcat tgcagcacta tggctctatt
      541 aagtgcttct ttttacttta tagatcgatg tcaacccagc gcgctttttg attgctttct
      601 atggcagctt cgatgatttg aacaatgtgg tggccttgga gaaaatctgc ataaagcggc
      661 ggctcgctga ataggattga gcgatagaag ttatcgatca ttctctgttg cacttccaaa
      721 aacctctcct gtcttgaagc accagccttt gcttccttgt tttcttccat ctcattcgca
      781 ggcgatataa ttcctttttc atccaaaatt aattctttct cttctcggta tttaagcgat
      841 gctctttctg aatgctcttg agaccaacgc aaggatgctg tgctaccatc tatctcaaag
      901 gagatgccat tagaccgacc agcacttacc tgagatacag ttaggcagcc atgaatgcca
      961 ccttcaaatc tcatcagcgc gcttgcaaaa tcctcagtat ctaccgttat agtctcagag
     1021 ggaaagcgaa tccgcttggg aatgaatgtt gcaaagtctg cggcgatgct ttctagtctg
     1081 cgatcaagca aaaattccgc tatatctatc caatgagagc caatatcagc aagggctctc
     1141 gatggtcctc catagcgaga ttcgacccgc cagtcgtaat catggtcgaa caagagccaa
     1201 tcttgaagat aagtcccgtg tatggaatag attgttccaa gctcgccctt ggatatcaat
     1261 tcttttatat gcataatgat tggataatat cgataaacaa aattgacagc ggtttttaga
     1321 tgacgattct ttgctaaagt cataagcgct cccgattgct gtgaatctat tgtgagcggc
     1381 ttttcagata gagcatataa gcctgcttcg agaatacctc tatttacatc atagtgaagc
     1441 acattgggag tacagttatg cacgagatcg agactctcgc tcttaagcat ttcttggtat
     1501 gaagtatacc aaacagggat atcgtagtcg tcgcagaggc tctttatctt gtttggattg
     1561 agagaacaaa tcgctgccaa ttctgcgtag ccgagaggct cgatagcttt gatagccttg
     1621 agatgagata tgcctatctt gccaattcct ataatcgcca ctcgaagttt tttcatagct
     1681 atttctggag cgctgatata agctcttttg ctctagcaat ctgatcgtag gataatggta
     1741 ttacattgct gttctgttgc gatgtagatg cagattcaat aaggatgagt ttggtttcct
     1801 gttgcgtaat agtattgtgc caaagcccct taggaatatt gtatactttg tttagctcca
     1861 tggagatagc cttcagttcg atgctatcat taattaggtc ctctaacgcg catagcagga
     1921 tacaagtacc gcgcaataat ataaagagct cgtcggtctc attgtgacgt tccaaacaat
     1981 cgatattact tatatcattc tctggtttat aattcttgat tccaaccatc cattccttgt
     2041 tttcatatac tctttggagg cctttccctt catgttcata ttctattata ccactcataa
     2101 ataattacct cttgaattac ttccttttta ctccaacaac atgatgcatt gttgcgcgat
     2161 atcatctgct gtaagaccat aggccttgaa aagatcttca ggttttccag aacgtccaaa
     2221 tttatcctgg ataccaacac gggcaaactt tatatttgat ttgcctacaa gaacttcagc
     2281 gacagctgac cctagccctc ctatgatcga atgctcctca gcggtcacca tggctcgaca
     2341 ttgaaaagcc atttctaaaa tcaactcagc atcgattggc tttatggtgt gcatattgat
     2401 aacgccagcc tttatgccat taagagataa tctttcagca gctgcgagtg cttctttcac
     2461 catgagccct gtagccacaa tgcagacatc accaggttca cgcatgatat ttgccttgcc
     2521 aattgcaaaa gcactgtctt catttgttat tacttcgaca accggccgag caacgcgaat
     2581 atataccggt ccatccaagc tcgcggcagc gaaaaccgcc ttgcgggttt caaaagggtc
     2641 acaaggaaca aggattgtca ttcctggtag cactcgcatt agtgcgatat cttcgatgct
     2701 ctgatgactt gcaccatctt cgcctacaga aagtcctgag tgagagagcc caaattttac
     2761 atttgcattg ggatatgcga tggaattacg aatttgctca tatgctcttc ctgcaccaaa
     2821 gagcgcgaaa gtacttacaa aaggcaaaag ccctgaatgg gcaaaccccg cagctgcccc
     2881 catcatattc gcttcagcaa gaccaaagtt gaaaaaccga tcgggaaact tagatgcaaa
     2941 aatactcgtc attgttgcat gagaaagatc cgtatccagc accaccacct tttgattgat
     3001 tgaaccaagc tcggcaagcg cctcgccata ggcgaccctc aatgatttgc tctcaaacat
     3061 tttgcgttcc tttcagctcg cacattgctt gattgtattc ctcagtgttc atcggtttgc
     3121 catgccatcc aacattattc tccatgaatg agattcccct gcccttgact gtctctgcga
     3181 gcacgaataa gggctttcca ggcaccggtt cggaaatcgc ctttaccagc atgtcgacgt
     3241 tatgtccatc gacttttttg cactcaaaac caaaggagtg gaattttcca attatatcgc
     3301 ccaaggacat aacctgatcg ttggttccat ctatttgcaa gccattatga tcaagtataa
     3361 agatcaaatt atcgagctta taatgagctg cagacatagt tgcctcccac accatgccct
     3421 cttgaatttc tccatcgcca agcaccacgt agacatggta tggtgcacgt ttatatttgg
     3481 cagcgagcgc tagtcccaca gctacagagg cgccttgacc tagtgagcca gtcgatacat
     3541 cgatgccagg agttttgcgc atatcaggat gcccttgaag atggctatcg atctgcctta
     3601 ggttccagag atcattccga gggaaaaaac ctttatcagc aagaatcgcg tagagagcag
     3661 gacaaccatg gcctttcgac aagacgatcc tgtctcgcat ttcccacgat ggatttgtcg
     3721 gatctacatg cgcaaccgca taatagagcg ctattagtat ttccacacat gagagcgatc
     3781 cgcccgggtg accagattga catcgataga gcatttcgat aatgtcgcgc cttatagata
     3841 gcgccttttg ttctagcatg agttcatcca tcttgagtat tcctttatta tcctttcacc
     3901 gcgcctgcag ttaggccttc gaccagtctt ttttgagcaa agaaaaacat tatacaaaca
     3961 ggaattattg tgatcacgcc tccagctgtc agcatccccc attcgacacc taactgacca
     4021 atgaagtttt taagtgcaac gggaattgtc ctatttcgtt cattggtaaa catgacagcg
     4081 aaggtatact cattccacga agtcatgaaa atatatgcgc ctgttgcgac tatacaaggc
     4141 aaaagcatag gcagaataat ctttataaat gcctgaaaac gattcgctcc atccaccatt
     4201 gcagcctcct cgagcgatcg aggtaaatca tcgaggtagc cagtcagaag ccagacagag
     4261 aatgggatgg taaatgttgt atatgaaaga atcaaagagc ttggtgtgta gagtatgcca
     4321 attttgcgca taatagcaaa aagaggaata agcaataata ccgttgggaa catattgttc
     4381 gtaagaaaaa gtgtcatgaa gagctgtcgt cccttaaatc gatatctgga aaaggcatat
     4441 gcagcaagca aggaaacgac aagagacaac agagtcgtta cgacagcaac tagtaagcta
     4501 ttaatcatgg gttttataaa attgtattca gcaaatagct ttttgtatga gccaaaggtc
     4561 acctttgcag gccaatatgt gaccgtgctt gaatagagtt ctttttctgg ctttattgag
     4621 gtcacaaagg tccaataaaa aggaaagaga agaaaaatga gcatcatcgc taacaaaata
     4681 tgtcctggca aagcgttcaa caactttctt ttacgaggcg ttttataaga ggatttttgc
     4741 acttctctac tttgtagttc cattactttg cttcttcctg tgccttaaag aggttcttca
     4801 gataaggaat agaaacaaca gataggccga ttgtcagaaa aattgcaagc gtagaggcat
     4861 atccaaggtt aagtgtattc cctttattaa agacatatac gcttagagtc tgcgtagagt
     4921 acgctggccc accttctgtc aaattgaaaa tcacatcaac agaatttgca acccaaataa
     4981 tccttagaag agttgtg
//
LOCUS       DAOMZL010000102         4893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_16290_length_4893_cov_13.888177, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000102 DAOMZL010000000
VERSION     DAOMZL010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16290_length_4893_cov_13.888177"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="PLQ30_07290"
     CDS             complement(<1..1021)
                     /locus_tag="PLQ30_07290"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPW02288.1"
                     /translation="MNLPLIVFILSFVAVFLLGIPLAPGMMLSSAFYFLLSSNPAASL
                     DMVAMQFLTNLNASFVLIAVPLFVFMAEIMNSGKVTEMIFSFSSALVGKRKGALAHVN
                     IINSIIFSGMTGSALADASGPGNMELQAMKQQGYDDAYTCAITAASATIGPIIPPSIP
                     MVVYAMLSSTSIGYLFLGGIIPGLLVGIALLIYNAFISNKRNYPQGKQFPARQFWKFA
                     LQALPALFTVVILLGGIYTGVVTPTEAGALAAFYGILISVFVYRALGWKEFKQCLINA
                     VRTTGTLSLLVGSSFAFSYIVSIEQIPNVIATFLLGLTQNKYVMLVILNIALLILGCF
                     LDTTPI"
     gene            complement(1018..1506)
                     /locus_tag="PLQ30_07295"
     CDS             complement(1018..1506)
                     /locus_tag="PLQ30_07295"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPW02289.1"
                     /translation="MPVWLKKTGKFLVDCIELYIPILSFSTLFVSFVLQIFARKFFKP
                     LLWPEELSLICFVWTALLGGLYSKRMGTQVAFTMLYDAAKPKTKRIMRIVGNALLLVA
                     FLIILVPSWDYVQFMQYKKSDALGIPMNIAYFPFVVYLVDMIIRLSIDIAKDFGTLRG
                     TK"
     gene            complement(1616..2614)
                     /locus_tag="PLQ30_07300"
     CDS             complement(1616..2614)
                     /locus_tag="PLQ30_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid TRAP transporter substrate-binding
                     protein SiaP"
                     /protein_id="HPW02290.1"
                     /translation="MKKTIVAMMVLLLLASSTAFAQQKPIKLVFTSVSVPTDAHTQAM
                     YVFEEEVERLSGGQIQVDVYDSGKLFTQQAEQDAIRKGTVDMVYSSASWLAEFVPYLS
                     MFGAAYTFQSYDQMTKTFNGPIGKRMFDDVAKKTGVRPLCAFYLGTRQLNLIAKVGPV
                     TKPEQMKGVKLRVPQSPTWIAMGKALGANPTPMAFNELYMGLKTGAVDGQDNPLPTDK
                     NAKFYEVTKYIVLTNHLVDSVWPSINEKKWQSLTKQQQEWVLQAIEKARQVCDKTNLD
                     NEKEILDFFRQQGLVVIENPDRDAFAAYAKNFYLTEGKDISKDWDLTLYDEIQKLK"
     gene            complement(2743..3672)
                     /gene="dapA"
                     /locus_tag="PLQ30_07305"
     CDS             complement(2743..3672)
                     /gene="dapA"
                     /locus_tag="PLQ30_07305"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPW02291.1"
                     /translation="MAEYEKERYGKILIPLLTSFCEDGRVDFERTAALAEFIIDQKKG
                     DSLVLAGSTGEFYAMSFDERVRLFETVKNKVGDRIPIIAGVGAASTIETIQLAKIAEK
                     IGFETVMIVSPYYTKPNQEELYCHFMKVATSVSLNILLYNIPIYTGVNIDPSTVSRLA
                     EQPNIVGIKEEAELNPKQITAFLNCTPENFVIYNGDDTMILEAYAQGGEKRIGGVVSG
                     SSHIIGHLIRSMIDNFLTGKVQAAAEMQQRIYPFLKSMGQNNRINAAPLLKEALRLYG
                     FDAGYPRAPLKYGNPNEIENIRLAMMQLDLLGK"
     gene            complement(3693..4481)
                     /locus_tag="PLQ30_07310"
     CDS             complement(3693..4481)
                     /locus_tag="PLQ30_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPW02292.1"
                     /translation="MAKVNSVRKALAILDLLSDGKKRSLSDIARELCLAKSTVSNLLE
                     TLTTERILERNRESGLYSLGIRLIELGYCTQTGYDLVRIAAPFLRGLNQEFDETVHLV
                     LLDRDEVLYIDCIESHRRLRTYSVIGVRAPLHCTSVGKAILAFLSDSEIRGIISRKGL
                     LSFTSHTITTEERLWNEITIIREHGYAIDDMEHEDHLRCVGAPIFNVEGKVFASVSIS
                     GPAERNTLKRLESMAPALLEATTEISHRLGYRNKDQSRAGSQII"
     gene            complement(4651..>4893)
                     /locus_tag="PLQ30_07315"
     CDS             complement(4651..>4893)
                     /locus_tag="PLQ30_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02293.1"
                     /translation="IWKNVDLGHLAQARAGQAILNSAIQELTTLGLYQALKEIISISG
                     IDTGCCLPPFSPLSQEQIEKADALGRKYEDMKLIRL"
BASE COUNT         1366 a         1072 c         1071 g         1384 t
ORIGIN      
        1 gtatgggcgt ggtatctaga aagcaaccca gaataagcaa ggcgatgttg aggattacga
       61 gcatgacata tttattctgg gttaacccaa gtaagaatgt ggcgataaca ttgggtattt
      121 gttcgatact gacgatataa gaaaaggcaa aggagcttcc gacaagaagc gaaagcgttc
      181 ctgtggtgcg caccgcattg ataaggcatt gcttgaattc cttccaacca agcgctcggt
      241 atacgaacac ggatatgagt attccataga aggctgccaa agcccctgct tctgtgggag
      301 taacaacgcc cgtatatatt cctcccagca aaattactac ggtgaaaagt gcaggcaaag
      361 cttgcaaagc aaatttccag aattgcctag ctggaaattg ttttccttga ggataattcc
      421 ttttattcga tataaatgcg ttgtagatca ggagagctat gccaacgagt agtcctggaa
      481 ttatccctcc caggaatagg tatcctatcg atgtactgga gagcatagca tatacaacca
      541 tagggatgct cggcgggata attggtccaa tggtcgcgct agctgcggtg atggcacagg
      601 tataagcatc atcatagcct tgttgtttca ttgcctgtaa ttccatattg cctggacctg
      661 aggcatcggc aagcgctgaa cctgtcattc cagagaaaat gatcgagttg ataatgttga
      721 cgtgtgcaag agcgcctttt cgcttaccca cgagcgcgct agaaaatgag aagatcattt
      781 cagttacttt gcctgagttc attatctcgg ccataaagac aaaaagtggt accgcaatca
      841 ggacaaagct cgcattcaag ttggtaagaa actgcatagc gaccatatct agcgacgcag
      901 cagggttgct cgaaaggaga aaataaaatg cgctagacaa catcatccct ggcgcaaggg
      961 gaataccaag caggaatacg gctacaaacg acaatatgaa aacaataagt gggaggttca
     1021 tttagtccct ctgagcgtgc cgaaatcttt tgcgatatca atgctcaacc tgataatcat
     1081 atcgactagg tacacgacaa atgggaaata cgcgatattc atgggaatgc ccagtgcatc
     1141 tgatttctta tattgcatga actgcacata atcccatgaa ggaactagga tgattaaaaa
     1201 agctacaaga agcagagcat tgcccacaat gcgcataatg cgctttgtct ttggtttggc
     1261 agcatcatag agcattgtga atgcgacctg agtgcccatt ctcttcgagt aaagaccacc
     1321 cagtagagca gtccatacaa agcaaatcaa actcaattct tctggccata aaagcggctt
     1381 gaaaaattta cgtgcgaata tctgcagaac gaaagagacg aaaagcgtac taaaactgag
     1441 aataggtata tagagctcaa tgcagtcgac caggaatttg cctgtctttt tcagccaaac
     1501 cggcataatg cctcctcctt caaataccct ttcaaaaagc atcgatacga tatcgacata
     1561 cttattgaat caaggctgcc tgcagcagat ctctgctgca ggcaagctct aggtattatt
     1621 tcagtttttg tatttcatca tagagagtga ggtcccaatc ttttgagata tctttgcctt
     1681 ctgtgagata gaaattcttt gcgtaagctg caaatgcatc gcgatcagga ttttctatga
     1741 caacaagacc ttgctggcgg aagaaatcca ggatttcctt ttcgttatcg agatttgtct
     1801 tatcacagac ttggcgtgct ttttcaatcg cttgcagaac ccactcttgc tgttgctttg
     1861 tcaggctttg ccattttttc tcattgatag aaggccatac tgagtcaacc agatgatttg
     1921 tgaggactat atacttggtg acttcataga atttggcatt cttatctgtt gggagaggat
     1981 tatcctggcc atcaacagcg ccagttttga gacccatata caattcgtta aaggccattg
     2041 gggtcgggtt tgctccaaga gctttgccca tcgcgatcca ggtaggactc tgcggaacac
     2101 gcaacttcac acctttcatc tgctcaggct ttgttacagg gccaactttt gctatgaggt
     2161 tgagctgacg agtgccaagg taaaaagcac agagtggccg aacaccggtc ttcttggcca
     2221 catcatcgaa cattctctta ccaattggac cattgaaggt cttagtcatc tgatcatagc
     2281 tttggaatgt ataggctgcg ccaaacatcg aaaggtaagg gacaaattct gcaagccacg
     2341 atgcggaact atagaccata tcgactgttc ccttacgaat agcatcctgc tcggcctgct
     2401 gggtaaagag cttgccagag tcgtagacat cgacctggat ttgtccgcca gaaagtctct
     2461 caacttcctc ctcgaaaacg tacattgcct gtgtatgcgc atctgttggc acactgactg
     2521 aagtaaagac aagcttaata ggtttttgtt gggcgaaggc tgttgagctt gcaagtagaa
     2581 gcaataccat cattgcaaca atggttttct tcataaaata cctccttata tgcatcctct
     2641 atctccagcc gtacaaaaga cggctaaatg cctagctatt atctatacgg caatagatat
     2701 tgcctttatc agtctaattg gcaatgttta ggtctggttt cttcatttcc ccaagagatc
     2761 tagttgcatc attgccaatc ggatattttc aatctcattt ggatttccat acttaagtgg
     2821 tgctcgagga tatccagcat cgaatccata aagtcgaagc gcttctttca agaggggagc
     2881 ggcattaatc ctattattct gccccatgct cttcaggaat ggatagattc tctgctgcat
     2941 ttctgctgct gcttggactt tccctgtaag aaaattatcg atcatggatc ttataagatg
     3001 tcctatgata tgagatgatc ctgaaacaac gccacctatg cgtttttcgc caccctgggc
     3061 ataagcttca aggatcatag tatcatcgcc attgtagatg acaaaatttt caggtgtgca
     3121 attcaaaaaa gccgtgatct gttttggatt cagctcagct tcctctttga ttccgactat
     3181 atttggctgt tctgcaagac ggcttactgt agatggatca atattaacac ccgtatagat
     3241 aggaatgttg tataggagta tgttgagaga taccgatgtc gctaccttca tgaaatggca
     3301 atatagctcc tcttgatttg gttttgtata atatggagat acaatcataa ccgtttcaaa
     3361 accgattttt tcggctattt ttgccaactg aattgtttcg attgtagaag ctgctccgac
     3421 gcccgctatt atggggattc gatcaccgac tttgtttttg actgtttcga ataaccttac
     3481 cctttcgtca aacgacatag cataaaattc gcccgttgat cctgcgagga caaggctatc
     3541 acctttcttt tgatcaataa tgaattcggc aagagcagcg gttctttcaa agtctactct
     3601 gccatcttcg cagaaggatg taagcagagg tatcaatatc tttccatagc gttctttttc
     3661 atattctgcc ataaaaacct ccttaaatcg gattatatta tttggctgcc tgcgcggctt
     3721 tgatccttgt ttcgatatcc aagacgatgc gatatctccg ttgttgcctc caaaagggct
     3781 ggagccatcg attcaagtcg ctttagcgta tttctttcgg caggacccga tatgctgacc
     3841 gatgcaaaga ccttaccttc cacattgaag atgggggctc ctacgcagcg cagatgatcc
     3901 tcatgttcca tatcatcgat tgcgtaacca tgttcacgga taattgtaat ttcgttccag
     3961 aggcgctcct cggtcgttat tgtatgggat gtgaaggaca gcaagccttt ccttgagata
     4021 ataccccgta tttcggaatc tgataaaaaa gcgagaatag ccttgccaac agatgtgcaa
     4081 tggagcggcg cgcgaacacc aataacagaa taggttcgaa gtcggcgatg cgattcgata
     4141 caatcgatat agagcacttc atcacgatcg agcaaaacaa ggtgtactgt ttcatcaaat
     4201 tcctggttga gaccgcgcag aaatggagcc gcaattcgca caagatcgta cccagtctgt
     4261 gtacaataac ccaattcaat aagtctaatg ccaagcgaat acaggcctga ttcgcgattt
     4321 cgctcaagta ttctttcagt agtaagtgtt tcaagcaggt tggagactgt gctttttgca
     4381 agacatagct cgcgcgcgat gtctgacagg ctccgctttt ttccatccga aagaagatcc
     4441 aggattgcga gtgcttttcg aacagaattt acttttgcca ttcaaatctc tacagaattg
     4501 ttcatatata agaacaatgt tcttatatat gaattgtctc atgggaatta gatgttgtca
     4561 agaagaattc atacaaagaa taactttctg tacacaataa attcggtgca caactccatg
     4621 ccaagcatat agaaaaaata caactattgc ttaaagcctt atcaatttca tatcttcgta
     4681 tttccggcca agcgcatcgg ctttttctat ttgctcttgc gatagcggcg agaagggcgg
     4741 caggcagcat cctgtgtcaa tgcctgatat ggatattatt tccttcaggg cttgatagag
     4801 gccaagggtt gtaagctctt ggatggcgct gttgagtatg gcttggccag cgcgcgcttg
     4861 agcaagatga ccaagatcca catttttcca gat
//
LOCUS       DAOMZL010000103         4877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_16360_length_4877_cov_13.930734, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000103 DAOMZL010000000
VERSION     DAOMZL010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16360_length_4877_cov_13.930734"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /gene="mtnA"
                     /locus_tag="PLQ30_07320"
     CDS             complement(<1..977)
                     /gene="mtnA"
                     /locus_tag="PLQ30_07320"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPW02294.1"
                     /translation="MQIPLTMRLDDEKGELIILDQSMLPNEVKYLRLSGTEEIWEAIQ
                     TLRVRGAPAIGIAAAYGAYLAVRNAQPSTMEDAIRIFMEARARLATSRPTAVNLFWAL
                     DRMQKRLEKEISQSSVQRLCTALREEADAIRKEDAQVCRCIGEYSLELLERGWGLLTH
                     CNAGVLATSEYGTALAPIYLGQNRGYGFRVFADETRPLLQGARLTAWELMQAGVDVTL
                     ICDNMAASIMKKGWVQAVLVGCDRIAANGDTANKIGTLGLAILAAHFGVPFYVHAPRS
                     TIDLNCATGIEIEIEERAAEEITTKWYEKPMAPEGVKAFNPAFDVTDAEL"
     gene            1124..2017
                     /gene="lipA"
                     /locus_tag="PLQ30_07325"
     CDS             1124..2017
                     /gene="lipA"
                     /locus_tag="PLQ30_07325"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPW02295.1"
                     /translation="MKNSIPDPKPSWLKIRLPHGQNWRHVEEVLASHGLHTVCDEAHC
                     PNKGECWGSGTATFMIMGDICTRGCSFCAVRAARFGRPLDPEEPQHLAKAIESLGLSY
                     AVITSVDRDDLSDRGSAHFSACIRAIRDLSPSTRVEVLIPDYIGQELKEVLSAKPDVI
                     AHNIETIRRLQSVRDQRASYNKSLQTLTEAASTGIPTKSSLLLGLGETEGEVLEALKD
                     LRSAGVSLIVMGQYLQPSPNEIPVKEYISPDRFDRYAEIARVLGFDSVLSAPFARTSY
                     HAREAWNASKKEHKNEARTDR"
     gene            1995..2291
                     /locus_tag="PLQ30_07330"
     CDS             1995..2291
                     /locus_tag="PLQ30_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02296.1"
                     /translation="MKLELIGKPEGCKLLRMTLEVEPSLSTSSRIISLSIRGDFFAIP
                     EEAFDEIQDELRGITIGEFAECFDRLAQEKALEFEGISGEGLMELIQKELNHDS"
     gene            2281..3063
                     /locus_tag="PLQ30_07335"
     CDS             2281..3063
                     /locus_tag="PLQ30_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HPW02297.1"
                     /translation="MTHKFRLLETGFHPAVFNMGLDEAILDSVSQGNCLPTLRFYGWI
                     PPAVSLGYFQGLQEEIDIEACKALGIDVVRRITGGGAVFHHHEVTYSIVIPLGHSLAR
                     SGIIDSYRILLAGIVEGLAELGIQSEFAPINDIVSAGKKISGNAQTRKKGCILQHGTI
                     IVEVDIDLMFSILKVPQEKTKGKLIEDAKSRVTSIRNCLSVPDSVSFEELFFSTITAL
                     ERGYSRALDLDYELSGPTDLELQNAERLAREKFSNNQWTALR"
     gene            3156..4871
                     /locus_tag="PLQ30_07340"
     CDS             3156..4871
                     /locus_tag="PLQ30_07340"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HPW02298.1"
                     /translation="MKKETKPQYVIGIDYGTDSCRAVIIDAANGYEISSAMAAYPRWS
                     KGLYCDPGKNQFRQHPMDYIESLEIVMQQIHDKTGQDVLPFVEGIAIDTTGSTPCLIT
                     DTLCPLAMLPEYKDNPDAMFILWKDHTSTEEAFEITELARRWSVDYTMYEGGTYSSEW
                     FWAKLLHIARTDRHVMNAGFSAIEHCDWMPALLTGTNALPKIKRSRCAMGHKAMWHES
                     FGGYPSDDFFAALDMQLVRIKKTLGRDTYTSDTEFGKLSNEWAERLHLRSGIPVAVGA
                     YDAHVGAVGGGVEENVLVKVMGTSTCDMIVGPRASSENPETSEHLVKGICGQVDGSII
                     PGMIGYEAGQSAFGDVYAWFKDLLSWPLKEMKSESMESAKELGIPSNTLDDLADSIDS
                     RLIFAIDKAASKIQPEESSVLALDWLNGRRTPYANQKLKGAIIGLSLGTTAPMIYRAL
                     IEATAYGSRAIIERFEQENIHIEKVRAVGGVARKSKLVVQIVADVLGRPIEIIASDQS
                     VALGAAMFASVVAGIYDSVTQAQLAMKPDIETVIQPDFVRTSLYDALYRDYLKLGAFI
                     ENELT"
BASE COUNT         1342 a         1153 c         1126 g         1256 t
ORIGIN      
        1 agctcggcat cagttacatc gaaggctgga ttgaatgcct tgacaccttc tggagccatg
       61 ggcttttcat accactttgt cgtgatctcc tcggctgccc gttcctctat ttcaatttca
      121 attcctgttg cgcaattcaa atctattgtt gatctcggcg catgaacata gaaaggaact
      181 ccaaaatgtg cagcaagaat ggcaagtcca agagtaccta tcttattcgc cgtgtctcca
      241 tttgccgcaa tgcgatcgca acctacaagt actgcttgta cccacccttt tttcatgatg
      301 cttgctgcca tattatcgca gataagggta acatcaaccc ctgcttgcat gagttcccat
      361 gccgtcaacc gagctccttg cagtaatggc cttgtctcgt ctgcgaatac tcgaaaacca
      421 taacctcgat tttgtccaag ataaattgga gcaagagctg taccatattc tgaggtggct
      481 aggactccgg cgttacaatg agtaaggagt ccccatcctc tttcaaggag ctccagagag
      541 tattcgccta tacatctgca cacctgtgca tcctccttgc gaatagcatc ggcttcctct
      601 cttagcgcgg tacaaagcct ctgaaccgag gattgcgata tttctttttc cagccgtttc
      661 tgcattctat ccaaagccca gaatagattg accgccgtag gccttgaagt ggcgagacgg
      721 gctcgagctt ccatgaatat acgtattgca tcttccatgg ttgagggctg agcatttcgg
      781 actgcaagat atgctccata tgctgctgca attcctatag ccggagcacc ccgaactcga
      841 agagtttgaa tcgcttccca gatttcctct gttccagaaa gccgtaagta cttcacctcg
      901 tttggaagca tcgattgatc gagaataatc aattcccctt tttcatcatc gagcctcatt
      961 gtcagcggta tttgcatatc gaaatttctc cttttcctgc ctttttaaat caaaaaacac
     1021 cttttacaca gaaggcgccc ctaacaataa tagggttata taatctggag tcaatcacga
     1081 tccatcatta cttttttagc agatccgtct ataatacgta ttcatgaaaa actcaattcc
     1141 cgacccaaag ccatcctggc tcaagatacg gcttccccat ggccagaatt ggcgccatgt
     1201 cgaagaagtc ctggcaagtc atggccttca taccgtatgc gatgaagctc actgccccaa
     1261 taaaggagaa tgctggggtt ctggtacggc caccttcatg ataatgggag acatctgcac
     1321 tcgcggctgt agcttctgcg cggtaagggc cgccagattt ggccgaccac tggatcctga
     1381 agaaccacaa caccttgcaa aagcaataga atcgcttggt ctctcctacg ctgtcataac
     1441 ttctgtggat cgagatgatc tgtcggatcg tggttctgcc catttttcgg cttgtatccg
     1501 cgcaatccgc gaccttagtc catctactcg agtagaagta ctaattccgg actatattgg
     1561 acaggagctc aaggaagtac tatctgccaa accggatgta attgcgcata atatagaaac
     1621 cattcgccgt ttgcagtctg tgcgcgatca aagagcttcc tataataaga gcctgcagac
     1681 cttaaccgaa gccgcgtcta caggaatccc cacaaaatcc agcctattgc tcggtcttgg
     1741 agaaaccgaa ggagaagtgc tcgaggctct caaagatctt aggtcggcgg gtgtatctct
     1801 aattgtgatg ggccaatact tgcagccatc gccaaatgag attcctgtaa aagaatatat
     1861 cagccctgat agattcgaca gatatgcaga aatagcgagg gttctgggtt ttgactctgt
     1921 gctgtccgct ccattcgcca gaacgagcta tcatgcccgc gaggcttgga atgcctcaaa
     1981 aaaggagcac aaaaatgaag ctcgaactga tcggtaaacc agaaggctgc aagctcttgc
     2041 ggatgacact cgaggtagag ccttccctat caacatcctc gaggatcatt tccctttcca
     2101 tccgaggtga tttctttgca attccagaag aggcctttga cgaaattcag gacgagctcc
     2161 gcggaataac tatcggagaa ttcgccgaat gcttcgatag actcgcccaa gagaaagctc
     2221 tagaattcga aggcattagc ggagaaggac ttatggaact cattcagaag gaactcaacc
     2281 atgactcata aatttagact cctggagacg ggctttcatc ccgcagtatt caacatgggt
     2341 ctcgacgaag cgattctcga ttccgtgtca cagggcaact gccttccaac cctgcgcttc
     2401 tatggttgga ttcctccggc cgtctcctta ggatattttc aaggtttaca ggaagaaata
     2461 gatatagaag catgcaaagc tctaggcatt gatgttgtta gacgcatcac tggaggagga
     2521 gccgtatttc atcatcatga agtgacatat agtattgtaa ttcctctagg acattctcta
     2581 gcacgctcgg gtataatcga ttcatatagg atattgcttg ctggcatagt cgagggtctt
     2641 gcggaactcg gcattcagtc tgaatttgct cctattaatg atatcgtaag tgcgggaaaa
     2701 aagatctctg gaaacgcaca aaccagaaaa aaggggtgca tacttcaaca tggcaccatc
     2761 atagtcgagg tcgatatcga tcttatgttc tcgatcttga aagtcccaca ggaaaaaacc
     2821 aaaggtaagc tcatcgaaga tgcaaagtca cgtgtaacta gcatacgcaa ctgcctttct
     2881 gtgcctgatt cagtgagctt tgaagagctt tttttctcta caataacagc acttgaacga
     2941 ggttattcac gggcgctcga tcttgactac gagctatccg gaccaacaga tctcgagcta
     3001 caaaatgctg aaaggcttgc ccgagaaaaa ttctccaaca atcaatggac agcactgcga
     3061 tagctcaaac gcaaatcttg cttttgatcc gatcttgtac ccttgctttg atcgatcttt
     3121 cggtataata gctaagttga agcacggaga tttcgatgaa aaaagaaact aagcctcagt
     3181 atgtcatcgg catagactat ggaaccgatt cgtgtcgagc cgtgataata gatgccgcta
     3241 atggttacga aatatccagc gcaatggctg catatcccag atggtcaaag ggactctatt
     3301 gcgatccagg aaagaatcaa ttcaggcagc atccaatgga ttatatcgag tcacttgaga
     3361 tcgtaatgca acagatacac gataagaccg ggcaagatgt cctgccattc gtagaaggca
     3421 ttgctatcga taccacaggc tcgacgccct gtctcataac tgataccctc tgccctctcg
     3481 cgatgctccc tgagtataaa gacaaccctg acgctatgtt catattatgg aaggatcata
     3541 cttcaacaga agaagccttt gagataaccg agctggcgcg aagatggagc gtcgattata
     3601 cgatgtatga gggcggcaca tactcttcgg aatggttctg ggcaaaactt ctccatatag
     3661 cccgcacaga ccgccatgtc atgaatgctg gcttttctgc aatagaacat tgtgattgga
     3721 tgcccgcgct actgacggga acgaacgctc tcccaaaaat aaagcgaagt cgttgtgcaa
     3781 tggggcataa ggcgatgtgg catgagagtt ttggaggcta tccgagcgat gattttttcg
     3841 ctgctctcga tatgcaacta gtacgaatca aaaagacttt gggaagagat acctacacaa
     3901 gcgataccga atttggaaag ctttcgaatg aatgggctga aaggcttcac cttcgatccg
     3961 gtattccggt ggcggttgga gcctacgatg ctcatgtagg agcagtaggc ggaggagtag
     4021 aagaaaatgt gcttgtgaag gttatgggta ccagtacctg cgatatgata gttggaccaa
     4081 gagcatcttc ggaaaatcca gagaccagcg agcatcttgt aaaaggaata tgtggccagg
     4141 tcgacggttc tatcattccc ggaatgattg gatatgaagc tggccaaagt gcttttggcg
     4201 atgtctatgc ctggttcaag gatcttctct cttggccgct aaaagagatg aaatccgaat
     4261 caatggaatc agcgaaagag ctgggcattc cttcaaacac tctggatgat cttgcagaca
     4321 gcatagattc tcggctcata ttcgcaatag ataaggccgc ttcaaaaatc caaccagaag
     4381 aatccagtgt tcttgctcta gattggctaa atggaaggcg cactccttat gcaaaccaga
     4441 aactaaaagg ggcaatcata ggcctaagct tgggcactac tgcaccaatg atatatcgtg
     4501 cacttataga agctacggct tatggttcac gagctatcat agagcgtttt gagcaagaga
     4561 acatacatat agagaaggtt cgggctgtag gcggagttgc gcgaaaatct aaactggtgg
     4621 ttcagattgt cgccgatgtt cttggtcgcc caattgagat tatagcgagc gatcaatccg
     4681 tagccctggg cgcggcaatg tttgcatcgg tagtggcagg aatctatgat tctgttacgc
     4741 aagctcaatt ggcaatgaag cccgatatag aaacagttat ccagccagat tttgttagaa
     4801 catcgctgta tgatgcctta tatagagatt acctcaagct tggagccttt atagagaatg
     4861 agcttacata aaagccc
//
LOCUS       DAOMZL010000104         4853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_16468_length_4853_cov_11.775323, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000104 DAOMZL010000000
VERSION     DAOMZL010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16468_length_4853_cov_11.775323"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PLQ30_07345"
     CDS             <1..860
                     /locus_tag="PLQ30_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02299.1"
                     /translation="GQYTKDRIVDKNGSRIVNGGAYYYGAAAAAAMGLKVAAITRLAE
                     EDFPSFSPLIERGVRVKAIATPKSTCLRLFYPSNNPDERILSVTSIAQPFEPSDIESV
                     SAPIWSIGASIRGEVSLEVLKAIKAKNARIGLDAQGFVRVVRDGQLAYDSDWPEKREV
                     LSLVDVFKADVVEAEILTGTRDLYEAARILARFDVPEFVLTHGGGVVVYAEGSFFEAP
                     FLPKTMEGRSGRGDTCLSSYLSARIFMPPKAATLWAAALTSLKMETLGPFLGTRDDVE
                     QAIRERYSI"
     gene            891..1667
                     /locus_tag="PLQ30_07350"
     CDS             891..1667
                     /locus_tag="PLQ30_07350"
                     /inference="COORDINATES: protein motif:HMM:NF022789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HPW02300.1"
                     /translation="MEIALSFVLAGVWISGATLLGEKLGSKRAGLITNLPSSILLSLI
                     FMALTMGPEYAAATTKGIPVGMTIDTLFTFVFILMARKGLSKALTISLIVWAITAYVF
                     IKLIPPLGMAGAIGLYVLVTALCFILAEGVLHIRSVPHKETTFSWKQILLRALFAGTV
                     VAGAVTIAQFAPPYMTGILATFPAVLSSTLVIFTLSQGADFARATGKILILSSSNIII
                     YAWIAGLTFPSLGPWIGTILSFATSVAYVALLGKLTAKIK"
     gene            complement(1851..3368)
                     /locus_tag="PLQ30_07355"
     CDS             complement(1851..3368)
                     /locus_tag="PLQ30_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPW02301.1"
                     /translation="MTRRIGKIAVLIMAIFALTFTGIVSAQDQLPGNVTITFAHTNDM
                     HGRIVEGADVLGFPKIYSAVEQLKASNPNTLLIDAGDTFHGLPVVNIDRGETAVKLMN
                     ELGYVYMTTGNHDYNYGFDRLMELSKMAEFKILAANVYKDGERVFPAYDIREMGGVRV
                     AFFGLATPETAYKADPKGIEGVTFGDPIVEAKLIVAELAGKYDLLVCISHVGIDDSSD
                     PTSITVAKFVPQIDVIIDGHSHSTLADIEKKNTTSTLITSTGSYGAGLGVVDIVVGPD
                     RKVISKTARTITPKNSPDLKANPSIAKVLDDLVKAQDAVLSQVVGRTEIKLEGTREIV
                     RTQQSNLGTLIANSMLYATGADFALMNGGGIRDSIPVGDITMKQIYTVQPFGNFIQSG
                     KVLGKEIDAILENGVGKLPAPDGRFPHVANLTYTLDASKPAGDRVSNIMIGGEPVDPE
                     KEYTIATLNFIFNGGDDYRMLIGKAQNDYPTDAEVFMSYIKYLGTITNDNIVFKK"
     gene            3539..>4853
                     /locus_tag="PLQ30_07360"
     CDS             3539..>4853
                     /locus_tag="PLQ30_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPW02302.1"
                     /translation="MAQKKIKTRGELSRKTVLLWALIALVLSALTNGYHYSLEGAKGL
                     YVWHPLIILKILLYMGLGPITIAILSLPLEKLAKKWPNIALRWASFVSSLLVSIVSIT
                     LFAFLIIVPRASSLEPARLKLIDPSKGVFPQESFEAPSFSRLENDDIDSTDSTGSGIH
                     AGSDSSADSAIHSHSMLPASIDTPLLKLSFSSDPHWGAETANAGARVRILEELADRRP
                     DAFFMLGDAVETGNNSTQWNFALSDLEAITPDIALRPIMGNHDALFGGQYLYKKAFWP
                     KDFVSDSGSPYYWSISSGPATIVAVDLPWGTEMWDARQKAWLEKTLAAAEPHKPLIVI
                     SHSYFYASGYEDPDFGSPWYDHYQNIPALVPLFEKYGVDLVVSGHNHYQELLSHNGVT
                     YAIVGSMGGIPDPEPSYRSPCSQWIEVGGYGWLDVEVLPGQLTLTF"
BASE COUNT         1215 a         1169 c         1131 g         1338 t
ORIGIN      
        1 tgggtcagta cacaaaggac agaatcgttg ataaaaatgg ttcacgcatt gtaaatggcg
       61 gagcgtacta ctatggcgct gccgcagcgg ctgccatggg attgaaggta gcagccataa
      121 cacgcctggc agaggaagat tttcccagct ttagcccact catagaacga ggcgtaaggg
      181 ttaaagccat tgctactcca aaatcgacat gccttagact attctatcca tcgaataacc
      241 ctgacgagcg cattctttcg gttacttcta tagctcagcc tttcgagcct tcggatatcg
      301 aatcggtttc agcaccaatc tggtctatcg gcgcttcaat ccgaggcgaa gtgagtttag
      361 aggtcctaaa ggcgatcaag gccaaaaacg cgcgtatagg tctcgatgca cagggatttg
      421 tgcgagtcgt acgagatggt cagcttgcat atgactcgga ctggcctgag aaaagagaag
      481 tcctctctct agtagatgta ttcaaagcag atgtcgtaga ggcggagata ttgacaggaa
      541 ctagagatct gtatgaagct gcccgtattc tcgctagatt tgatgttccg gagttcgttc
      601 tgacacacgg aggtggcgtg gtggtctatg ctgaaggatc tttcttcgaa gcaccatttc
      661 tgccaaaaac aatggaagga agaagtggcc gaggagacac ttgtctatcc tcttacctta
      721 gcgcgcgcat ctttatgcca ccaaaagcgg ctaccctctg ggcagcggcg ctgacaagcc
      781 tcaaaatgga aactctgggg ccatttttgg gcacccgcga tgatgtcgag caagctatcc
      841 gagaacgata tagcatatag aatggaggtg tagttatatc cagatctgtc ttggaaattg
      901 ccctttcctt tgtactggcg ggtgtgtgga tttccggggc tacactcctt ggagaaaagc
      961 ttggcagtaa gagagcgggg ctgattacta atctaccatc cagtatcctt ctatcgctca
     1021 tatttatggc acttacaatg gggccggagt atgcggctgc cacaactaaa ggtatccctg
     1081 tcgggatgac catcgatacc ctctttacat tcgtttttat cctgatggca agaaaagggc
     1141 tgagtaaagc ccttactatc agcttgattg tatgggcaat aacggcctat gtatttataa
     1201 aattgattcc accacttgga atggcgggcg cgatcggtct atatgtccta gtgactgcct
     1261 tatgctttat tcttgcagag ggggttttgc atatccgctc agttccgcac aaggaaacga
     1321 ctttttcctg gaagcaaatt cttttaaggg ctctttttgc cggaacggtc gtggccggag
     1381 ccgtgacgat agcgcaattt gcgccgccct atatgactgg cattttagca acttttccag
     1441 ctgtgctttc tagtaccctc gttattttca cattgagcca gggagcggac tttgcacgag
     1501 ccactggtaa gatactcatc ctttcttcga gcaatatcat tatatatgca tggatagcag
     1561 gtttgacctt tccatcgctt ggaccatgga taggaactat cctttccttt gcaacctcgg
     1621 tagcttatgt tgctctgctt ggtaagctta ccgcaaagat caaataagtg agcctaaagg
     1681 cgctgctcag aaggatttgc caaaggccag cttccatcgt attctagata atcaggatat
     1741 caggatccat aaaatgggag gccacccaaa tcttcggata gcctccctaa atccaatcaa
     1801 gacatcatat cagctgatag gaatgcgtaa aatgcctctt tatgctttcc ctatttcttg
     1861 aatacaatat tgtcgtttgt tatggtgcca agatatttga tataggacat aaagacttca
     1921 gcgtcagttg gatagtcgtt ctgggcctta cctatgagca ttctataatc atctccacca
     1981 ttgaagataa agttcagcgt cgcgatcgtg tattccttct cgggatctac tggctcgcct
     2041 cctatcataa tattcgaaac ccgatcacct gctggtttcg acgcatcgag cgtataggtg
     2101 agattggcca catgcgggaa gcgaccatcc ggagctggta gcttgccaac accattctct
     2161 aggatagcat cgatctcctt gcctagaact ttgccgctct ggataaagtt gccgaatggc
     2221 tggacggtgt atatctgctt catcgtgata tcgccaacag gaatggaatc tcgaatacca
     2281 ccaccattca tcaaagcaaa atcggcacca gtggcataga gcatcgaatt ggcgatgagg
     2341 gttccaagat tgctctgttg cgttcggaca atctctcgcg tgccttcgag cttaatttcg
     2401 gttctgccca caacctggga gagcacggca tcctgtgctt tgacaagatc atccagcact
     2461 tttgcaatgg acggattcgc ctttagatct ggtgaattct ttggagtaat cgtgcgagcg
     2521 gtcttcgaga ttacctttcg atctggaccg actacgatat cgactacgcc cagccctgcc
     2581 ccataggagc cagtgcttgt tatgagagtg cttgtcgtat tcttcttctc gatatctgca
     2641 agagtactat ggctgtgtcc atcgatgatc acatcgatct gaggcacgaa cttggctact
     2701 gttatcgaag ttggatcgct tgaatcatct ataccgacat gcgatatgca gaccaacaga
     2761 tcatatttcc ccgcaagctc ggctactatc agttttgctt ctacgattgg atctccaaat
     2821 gtaacaccct cgatgccttt cggatcggct ttgtaggcag tctctggagt tgcaagtcca
     2881 aagaaagcca cgcgcacacc gcccatttcg cgaatatcat aggcaggaaa taccctctct
     2941 ccatccttgt agacattagc cgcaaggatt ttgaattcgg ccatcttgga gagttccatc
     3001 aggcgatcga agccatagtt gtaatcgtgg ttgccagttg tcatatagac atatccaagc
     3061 tcattcatga gctttacggc tgtctcaccc ctatcgatat tgacgacagg taaaccatgg
     3121 aatgtgtcac ctgcatcgat gagaagcgta ttggggttag aagcctttag ttgctcaacc
     3181 gctgaataga tcttcggaaa ccctaataca tcagcacctt cgacaattcg cccatgcatg
     3241 tcattcgtat gcgcaaaggt gatcgtaacg ttgccgggca gctgatcctg cgccgacacg
     3301 atgccggtaa aggttagcgc aaaaatggcc atgatgagca cggcaatttt tccgattctt
     3361 cgtgtcattg aaaacctcct aattaatctc tgtgttctta agaagcaata ttattatacc
     3421 cagctcttcc cctacagaca agcaaaaaga gataattgtt cgattctccc atcaaaaaag
     3481 ccagtcaaga cgccaagaat ggcttaccgc tggaatatat tgctgatata ttattttaat
     3541 ggcgcaaaaa aagataaaaa cgagaggaga actctctcgc aagacagtat tgctttgggc
     3601 tttgatagca ttggtcctct ccgctctgac aaatggctat cattatagcc tggaaggagc
     3661 aaagggcctc tatgtatggc acccccttat tatactaaaa atcttgctct atatggggct
     3721 tggaccaatt accattgcca tactgtcttt accgctcgaa aaacttgcta agaaatggcc
     3781 aaacatcgca cttagatggg catcttttgt aagctctttg ctggtcagca tagtatcgat
     3841 tacactcttt gccttcttga ttatagtgcc gagagccagt tcgcttgagc ctgcaaggct
     3901 caagctcata gatccatcta aaggggtttt tccgcaggag agcttcgagg ctccctcttt
     3961 ttcaagactc gagaatgatg atatcgattc taccgattcg actggttccg gtattcatgc
     4021 cggttccgat agttccgccg attcggctat tcattctcat tccatgctcc ctgcttccat
     4081 tgatacgcca ctgctaaaac tgtctttctc ttctgacccg cattggggag cggaaacagc
     4141 aaatgcaggc gcacgtgtta ggatcttaga agagctcgca gaccgaaggc cagatgcatt
     4201 ctttatgctt ggcgatgctg tggagaccgg caacaattca actcaatgga attttgcgct
     4261 gtcggacctc gaagccataa cgccggatat tgccttgcgt cctattatgg gtaatcatga
     4321 cgcacttttc ggcggtcaat atctatataa gaaggctttc tggccaaaag atttcgtaag
     4381 tgattcaggc tctccatatt actggagcat aagctctggc cctgctacga tcgttgccgt
     4441 tgatctccct tggggaaccg agatgtggga tgctcgtcaa aaggcttggc tcgaaaagac
     4501 acttgcggcc gctgagccgc acaagcctct tattgtgatt tcacatagtt atttttacgc
     4561 ttctggttat gaagaccccg atttcggctc gccttggtat gatcattatc aaaatatacc
     4621 tgcgctcgta ccacttttcg agaaatatgg tgtcgatttg gtcgtttcgg gccataacca
     4681 ttatcaagag ttgctttcgc ataatggagt aacctatgca atagtcggat ccatgggagg
     4741 aattcccgat ccggaaccaa gctatcgatc accctgctct caatggatcg aagtaggagg
     4801 ctatggctgg ctcgatgtcg aggttctccc cggccaattg acgctcacct tcc
//
LOCUS       DAOMZL010000105         4828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_16572_length_4828_cov_9.067672, whole genome shotgun sequence.
ACCESSION   DAOMZL010000105 DAOMZL010000000
VERSION     DAOMZL010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16572_length_4828_cov_9.067672"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /gene="htpG"
                     /locus_tag="PLQ30_07365"
     CDS             complement(<1..1159)
                     /gene="htpG"
                     /locus_tag="PLQ30_07365"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPW02303.1"
                     /translation="MPSYTFKTEVNQLLDLITHSLYSHKEVFLRELVSNASDAIDRLK
                     YLTVSDDAFKTLNFEPRITVALDEEAKTITVSDNGIGMDLQELADNLGTIARSGTKAF
                     LEQLAENERKASNLIGQFGVGFYSAFMIADQIEVVSLKAGTDKAFIWKSDGKTGFDIE
                     PAVRAGFGTDVIIYVNDEGEEFLSRWRLEELLKRYSNHIAWPIHLVSKEKTYAKDPAK
                     AETKIVDEQVNSASALWRRPKSELSDKDYIDFYKSLTGDEKDPLLWIHTKAEGTIEYS
                     SLLYIPSHVPADLYIPTHEQGIKLYVKRVFITDRELQILPPYFRFVRGIIDSDDLPLN
                     VSREMLQHNRVMMTIQQATLKKLFGELEQLAETNSEKFNTFISLFNPQIKEG"
     gene            1434..2480
                     /locus_tag="PLQ30_07370"
     CDS             1434..2480
                     /locus_tag="PLQ30_07370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPW02304.1"
                     /translation="MPEGLRRIKEREKRWYSRLAEGLASFFITVQVELKRLLTWGRTR
                     IVFAIIPQSERPPRKFEIGRFSLLIIVLTVVICVGAAIISIGAYGAKSIRVVSLEAQL
                     SDAYGTIDRMRESSENLISETTKFEEKLGQVIEIAQSRGKPALQRSASEEILKTAGLD
                     DPILGQFSDDPVSRDLLKLDSIAETLDKSLGALDDIAKILASQKDIMTEVPNIWPIKG
                     NLGHISMYFGQNENPFSKGQWYLHTGIDISTNRIGDSILATADGKVIDIHYDSSLGNS
                     VTIQHSHGFTTRYGHLRSFAVKKGQRVFQGDVIGYLGNTGKTTGPHLHYEVYLGTSLI
                     DPLRFLNIRKEYHE"
     gene            2473..2811
                     /locus_tag="PLQ30_07375"
     CDS             2473..2811
                     /locus_tag="PLQ30_07375"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPW02305.1"
                     /translation="MSSRDFSDCMIGEGTRVGGTIDAPGMVRIDGEFSGDIKSGASVI
                     ISKKAVVHAHINARQLVVAGKFSGSAVIEGGVRYAETARIEADCTGKFLIVEAGALVN
                     GQFARIDIED"
     gene            2883..4073
                     /gene="ricT"
                     /locus_tag="PLQ30_07380"
     CDS             2883..4073
                     /gene="ricT"
                     /locus_tag="PLQ30_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPW02306.1"
                     /translation="MSVEYNNHDIPEDILDMLGEEEFESPQVLKPIPKDIENLPQPIY
                     RLKTLHSSETFHAYYVSLDNNEHFMTICELEAALAGPRQEIHGVPPSEEAWNCKQGTV
                     SAAAEVLQPEAIQAMAIQGEGIEAEVDQAGIIQAEIIHPKSELIPNRALVIAPTKYGK
                     DLVMVLGVVHDFSMVNADELVAIERPATVDDYRRYLDNMEHEEEAFAKCKECIDSHNL
                     AMKLISAHCLLEDSKILFFFTAESRVDFRDLVKDLVGIFHARIELRQIGVRDEARVTG
                     GAGVCGRILCCHGISDKLNPVSIKMAKDQNLSLNSVKISGPCGRLLCCLSYEHQFYRD
                     ARRELPNEGVRFTYDGILFKVIEVNALLNKIRISGEDGRILDMDAARLKYLDSHWTII
                     EEES"
     gene            complement(4114..>4828)
                     /gene="aspS"
                     /locus_tag="PLQ30_07385"
     CDS             complement(4114..>4828)
                     /gene="aspS"
                     /locus_tag="PLQ30_07385"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689967.1"
                     /note="catalyzes a two-step reaction, first charging an
                     aspartate molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; contains discriminating
                     and non-discriminating subtypes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPW02307.1"
                     /translation="KAHGLAWMKVSESATLEGGVSKFFADHSRVVQGLGASPGDLILI
                     VADGKKNIACSALGAVRSQLGKDLGLCDPDVFAFAWIIDFPLFEWNEEENRWEAAHHM
                     FTMPQTQYHETLEADPGSVKGDLYDLVLNGYEVASGSIRIHDPELQKRIFRIVGLSDK
                     EAQEKFGFLLEAFKYGPPPHGGIAPGFDRLVMVMAGETSIKEVIAFPKNSFAISPMDQ
                     CPSEVDERQLKELHIRIVD"
BASE COUNT         1277 a         1013 c         1227 g         1311 t
ORIGIN      
        1 ttccttcctt gatttgggga ttgaatagcg aaataaaggt attgaatttt tcggaatttg
       61 tctctgcaag ctgttcgagc tcaccgaaga gctttttgag tgttgcctgc tgaatggtca
      121 tcatgactcg gttgtgctgc agcatctctc ggctcacatt tagaggcaaa tcatccgaat
      181 cgattatacc tcgcacaaaa cggaaatagg gaggtagaat ctggagctct ctatcggtta
      241 taaacaccct ctttacatat agttttattc cctgctcatg agttgggata taaagatcgg
      301 caggcacatg agagggtatg tataagagtg aggaatattc gatagttcct tctgcttttg
      361 tgtgaatcca gaggagtgga tctttctcat cgccggtcaa agacttgtaa aaatcgatat
      421 agtccttgtc ggatagctcc gacttggggc ggcgccagag agcgctagcg ctatttacct
      481 gttcgtctac gatcttggtt tcggcctttg cgggatcctt tgcataggtc ttctctttgg
      541 atacaaggtg aataggccag gcgatatgat tagaatatcg cttgagaagc tcttcgagcc
      601 tccagcgaga gagaaactcc tctccttcat cattgacata tataatgaca tctgttccga
      661 agccggctcg tacggcaggt tctatatcga aaccagtctt gccatcgctc ttccagataa
      721 aggccttatc cgtacctgcc ttgaggctta ctacttcgat ctgatccgcg atcataaagg
      781 ctgaatagaa accaactcca aattggccaa tcagattcga tgctttgcgc tcgttttcgg
      841 caagttgttc cagaaaggct tttgtgccgg atctagcaat ggttccaaga ttatctgcga
      901 gctcctgaag gtccattcca attccattgt cggagacggt aatagtcttg gcctcttcgt
      961 ccagcgcaac ggtaatgcgt ggctcgaagt tgagagtctt gaaggcatcg tccgagacag
     1021 tgaggtactt gagcctgtcg atggcatcgg atgcatttga aaccagctcg cgcaaaaaga
     1081 cttctttgtg cgaatagagc gagtgtgtta tgaggtccag aagttgattg acctcagttt
     1141 tgaaggtata gcttggcatt ttagaatctc ctgaaaagca ggtgttacgg tagaaaccga
     1201 aaattaggca atgcgtttta taatatagca aatttttggc caaaggtgaa ttcgagcgat
     1261 gttcgctatt ttatttggct acaatcgcac aagtctgcaa atatttattt ttattatttt
     1321 ataccagtgt ctttatgtcg attcgatagc taactcgatc ggctcgataa agtactacat
     1381 taattgctaa aaatttgcga taatttatca cttaggcaag gagaaagagc aaagtgccag
     1441 aaggcttgcg tagaatcaaa gagagagaaa agcgttggta cagccgtctg gcggagggtc
     1501 tggcaagttt ttttattact gtacaagtag agctgaagcg tttgctgaca tggggaagaa
     1561 ctcgcatagt ctttgctatt atcccacaga gcgaaagacc ccctcgtaag ttcgagattg
     1621 gccgtttttc tcttttgata attgtcttga ctgtagtgat ctgcgttggg gctgcaatta
     1681 tttctatagg ggcatatgga gcaaagtcca taagagtagt ctctctggaa gctcagcttt
     1741 ccgatgccta tggaactatc gatcggatgc gtgaatccag tgagaacctg atctcggaga
     1801 caaccaaatt cgaggagaag ctcggacagg ttatagagat tgcacagagt agaggaaagc
     1861 cggcgctgca gcgcagtgca agtgaagaga tacttaagac cgctggctta gatgatccaa
     1921 ttctggggca gttttcggat gatccggtga gtcgagacct tctcaaactc gacagcattg
     1981 cggagacatt ggacaaaagt ctgggagcgc tcgacgatat cgcaaagatt cttgctagcc
     2041 aaaaggatat aatgacagag gtgccgaaca tctggccaat caaggggaat cttggacaca
     2101 taagcatgta ttttggacag aacgagaatc ctttttctaa gggacaatgg tatctgcaca
     2161 cgggtatcga tatttcgact aaccgaatcg gagattctat ccttgccaca gcagatggaa
     2221 aggtcatcga tatccattat gattcttctc taggtaattc cgtcacaatc cagcatagcc
     2281 acggctttac gactcgatat ggtcacctcc ggagctttgc ggttaaaaag ggtcagcggg
     2341 tctttcaagg cgatgtaatc ggatatctag gaaatacagg taagacaaca gggccacatc
     2401 tgcactacga ggtatatctg ggcacaagtc ttatagatcc tctgcgtttt ctcaatatac
     2461 gcaaggagta ccatgagtag tcgtgacttt tctgactgta tgataggaga gggcaccagg
     2521 gtaggaggaa ccatcgatgc tccaggcatg gtgcgcatag atggagaatt ctcgggtgat
     2581 ataaaaagtg gcgcttctgt gataatctcg aaaaaagccg ttgttcatgc tcatataaat
     2641 gctcggcagc ttgtggttgc aggtaaattc agcggtagcg cagttatcga aggtggagtg
     2701 cgttatgcag agaccgctag aatcgaggct gattgcacgg gtaaatttct tattgtggaa
     2761 gcgggagctt tagtaaatgg gcagtttgcg cgcatagata ttgaagatta atgtcgagcc
     2821 gctgttagga ataaggtccg cgaggacgcg atattagatc cgctttaaat caggagtaaa
     2881 tgatgtctgt tgaatataat aatcatgata tcccagaaga tatactcgat atgcttgggg
     2941 aggaagagtt cgaaagtcct caggtattaa agcctatacc taaagacatc gagaacctgc
     3001 ctcagcctat ttaccggcta aagaccctgc attcgagcga gacttttcat gcttattatg
     3061 tatctttgga taataatgag cattttatga ccatatgtga actagaggct gccctggctg
     3121 gcccgcgcca ggagattcat ggagttccgc cttctgaaga agcttggaat tgcaagcaag
     3181 gcacggtcag tgcagccgcg gaagtccttc aaccggaagc cattcaagct atggccattc
     3241 aaggagaagg tattgaggca gaggttgatc aagcagggat tattcaagca gagattattc
     3301 atccaaagag cgagctaatc cccaatcgag cgcttgttat tgcacccact aagtatggta
     3361 aggatttggt gatggtgctt ggagtggtgc acgatttttc catggtaaac gcagatgaat
     3421 tggttgccat agaaagacct gctaccgtcg atgattatag gcgctactta gataatatgg
     3481 agcatgaaga ggaagcgttt gcaaaatgta aagaatgtat agattcacat aatctggcaa
     3541 tgaagcttat ctcggcgcat tgtcttttgg aagacagcaa gatattgttt ttctttactg
     3601 ccgaaagtcg tgtggatttt cgggatcttg tcaaggattt ggttggaatt ttccacgccc
     3661 gcatagaact caggcagatt ggggttcgag atgaggctcg cgttacagga ggtgccgggg
     3721 tttgtgggcg aattctctgc tgccatggca tttcagacaa gctcaatcct gtatcgataa
     3781 agatggcaaa agatcagaat ctttcgctga attcggtcaa gatctctggt ccatgtggaa
     3841 ggctcctctg ctgtctgagc tatgagcatc aattctaccg agatgcccgt cgggagctac
     3901 cgaacgaagg cgttcgcttt acttatgatg gaatcctatt caaggttatc gaggtcaatg
     3961 ctcttctaaa caagattcgg atctctggag aggatggtcg gatcctggat atggatgcgg
     4021 ccaggctaaa gtacctcgat agccattgga ctatcatcga agaagagagc taaagatagc
     4081 caagggaaag atgactaagc gaagataata ttcctaatcc acaattctta tgtgcagctc
     4141 cttgagttgg cgttcatcga cttcgctcgg gcactgatcc attgggctta tcgcaaatga
     4201 attcttgggg aaagcgatta cctcttttat tgaggtttcg ccagccatga ccataactag
     4261 cctatcgaag ccaggcgcta tgccaccatg gggcggtggt ccatatttga aggcttccaa
     4321 caaaaagcca aatttttcct gggcttcctt gtcgctaagg ccaactatgc ggaagatccg
     4381 cttctgcagt tctgggtcat gtatacgaat cgatcctgag gcaacttcat agccattcag
     4441 aaccaagtca tataggtcgc cttttaccga tcctggatcg gcttcgagag tctcatggta
     4501 ctgtgtctgt ggcatggtga acatgtgatg ggctgcttcc cagcgatttt cttcttcgtt
     4561 ccactcgaag agaggaaaat cgattatcca ggcaaaagca aatacatcgg gatcgcagag
     4621 acccaggtct ttgccgagtt ggcttcggac tgctccgaga gcgctgcaag cgatattctt
     4681 ttttccgtct gccactatca agatcagatc gccggggcta gcgccaagcc cttgcaccac
     4741 gcgcgagtgg tccgcaaaaa acttagagac gccgccttca agagtggcgc tctcgctcac
     4801 tttcatccag gcaaggccat gagccttg
//
LOCUS       DAOMZL010000106         4826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_16582_length_4826_cov_13.521274, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000106 DAOMZL010000000
VERSION     DAOMZL010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16582_length_4826_cov_13.521274"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..2254
                     /locus_tag="PLQ30_07390"
     CDS             206..2254
                     /locus_tag="PLQ30_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HPW02308.1"
                     /translation="MKKPIFYGILVLFGSFLLFGCPLAPKSSNAYLSDLRIEGATLDE
                     TFSRGKTDYTAFLSSSLSSAKIVAVAEDSKATISYNANPLTLLTGENTARITVKAEDG
                     TEKTYTITIWRGNTSINTLDSVAGSYLTQTIKYNVYKNGNLQYIIKKGLAENQPLYLD
                     PSATYKLIASAEKRASSSIENYHYSATSDPIYLVCQKLEMSTYPAEAPSITSLAWTSD
                     ESADINDLDSLKESNWHEFSDGDEINISSLNIVRVIAKAKSEMDATSWAGQGIMLGVD
                     EVPSYFAGLYPNNADSSYDNTTGYFTGEAYFGPLAGLQIADGEHQICAVVYDRSSNRV
                     ERSVSVTSISGPSSGVDLSSNYNICDLYVEARTYGVSRQYYGARKSPEAMQALDSGPA
                     SYRIWLSFYVQKNSNNLDQPILGFRVYRSENGGSSYKYLATVNYGYPVSGQHTYYDWD
                     GALRPEKSYKYKITAFTDSNNKHDLSESSAPIQLLAPYTISLTSPADGAKLSRGTIPT
                     LKFRLSETSLWSSDKSDGFAFMPAIRKKTGETIFCGMYMYDMESDMLSYWDMPDWNWK
                     EIVKTDSETLSDYFTFNKGTGEITLKQAALIADANLYSAGAPVLSAGVTYEWDIFGWW
                     SDGNDPGNSENVDPPYFFKEETAHDSRYRSIYSESYSFSDSYEDGMDALNGWFELIAE
                     "
     gene            2266..4407
                     /locus_tag="PLQ30_07395"
     CDS             2266..4407
                     /locus_tag="PLQ30_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPW02309.1"
                     /translation="MKKNIYRSIARGALLVSLLLILSGCNFFPKQGSQLQKAPDFYPE
                     YSMFQPKTLKEFDNAVQLGHVSSDTLIAKTSIDFDKDQFLKCELEVIGSFEVEGNRYF
                     RLHASQNIFKKAKRIEKLKGVLYVEHELRSYIPKNEQGPFIDSIASSKGAGEISAVLN
                     DPETWGRYGHFEITHAIDAYRDLGFGDNTVYVVDIDTGIDQTHEDFHLSSEESIIEYA
                     KSAFDEYGTYVDDMNPFIEVPANENWDDMGHGTHTAGIIAALGNNGIGVAGVAWKNVK
                     LISYKCFSEVSPYSGYDWPVYGGFADLVDWKKANAPEQTIPVNMSLGGYYASAFELEM
                     ISYALRNGIVIIASMGNDGTERAQYPAAYAGVIAVGATRANEEKVGFSTSGKHISVSA
                     PGYNIYSTFNDGGYADMSGTSMSAPFVTGLVAYMLTFNPDLTADQIKAILEQTAMDIG
                     PTGWDTGTGYGLVDVRKAVTKVKNNEIPLVGSIYASGEAVISVQNLDSEYDSGNTHYP
                     DAVGGVPVYLYGADGSFVAVGVSNYTDGSVSFRLLKPGDYTAKTNWGGVPKSQAFNMP
                     ATGNKTQSINYDMKIYYAQTLWNELMDPDYFSWADSVLTLYTEEGSLIVGPYDYSYLD
                     TLMIPVKSGQSYVLGIEPYFNDEYSWGEYGLIISQDLYDSVNTNNGRGSGDDDTFEPN
                     NDFASAKDIDVDIEYGLYLGDGDYFRFIIPE"
     gene            4603..>4826
                     /locus_tag="PLQ30_07400"
     CDS             4603..>4826
                     /locus_tag="PLQ30_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02310.1"
                     /translation="MEPSCIAAFGLWCGLAWGGVSGSGIWDLFLAFIIGAIAISISIG
                     HLKLSDKNGRRELLLWSALSMGLALSAGVLR"
BASE COUNT         1399 a          938 c         1154 g         1335 t
ORIGIN      
        1 gacgaaaaga attattccat aggcggaaat tcgcttaggg gaaaggtcga tcatgttttg
       61 attaagatta acatagaaga gcattttatt catctgtctc gtataaaaaa gcgattattt
      121 ttgcgtaaaa acttaaaata actattgctt tcattaataa ttgcactaat atataagaaa
      181 gtaaaggaaa aactaggagg ctatgatgaa aaaaccaatc ttctatggca ttcttgtgct
      241 ttttggatca tttttattat ttggttgccc tttagctcca aaatcaagca atgcatatct
      301 ttctgatctt agaatagaag gggcaactct cgacgagaca ttttcgcgag gtaagacaga
      361 ttatactgca ttcttatcaa gctcattgtc ttcagcaaag atagttgctg ttgcagagga
      421 ttccaaagca acgatctcct acaatgctaa tcctctaact ctattgacag gagagaatac
      481 agcaagaata actgtcaaag cggaggatgg cactgaaaaa acctatacca taactatatg
      541 gagagggaat accagcataa atacgctgga ttctgttgca gggagctatt tgactcagac
      601 cataaaatac aatgtctata agaatggtaa tcttcaatac ataatcaaga agggtcttgc
      661 ggaaaatcag ccgctttatc ttgacccgag tgctacctac aagctaatag cgagtgctga
      721 aaaacgagct tcttcatcta tagagaacta tcattattca gcaaccagcg atccaattta
      781 cctcgtgtgc cagaagctgg aaatgagcac ttatccagcc gaagctccaa gcatcacttc
      841 tttggcctgg acgagtgatg aatctgcaga tattaatgat ttggatagtc tgaaggaatc
      901 caattggcac gagttcagcg atggagatga aatcaatatt tcgagtctca atatagtaag
      961 agtaattgcg aaagcaaaat ccgaaatgga tgccacatcc tgggctggtc aaggaatcat
     1021 gctgggagtg gatgaggttc cgagctattt tgctggcctt tatccaaata atgctgattc
     1081 aagttatgat aatacaacag gatattttac tggcgaggcc tattttggac ctcttgcagg
     1141 attgcaaatt gcagatggag agcaccaaat atgcgcagta gtctatgata gaagcagcaa
     1201 tcgtgtcgaa aggagtgttt cggttacctc tatctctgga ccatcttcag gggttgatct
     1261 atcaagtaat tacaacatct gtgatctata tgtagaagca agaacctacg gtgtttcccg
     1321 tcagtattat ggtgcaagaa aatcgccgga agccatgcag gcgctagatt ctggcccagc
     1381 aagctatcga atttggctct cattttatgt gcagaaaaat agtaataact tggatcaacc
     1441 cattcttggc tttagggttt atcggtcgga gaatggaggt tctagctaca aatatcttgc
     1501 aacagtcaat tacggttatc cagtgtctgg acaacataca tactatgatt gggatggggc
     1561 tcttcggcct gaaaagagct ataaatacaa gataacggca tttaccgact ccaataataa
     1621 acatgatctc agtgaatcct cggcgcctat ccaattgctc gctccttata caatatcgct
     1681 tacctctcct gctgatggcg caaaattgag ccgcggaacc attccgacac ttaaattcag
     1741 gctgtcggaa acctcgctct ggagctctga taaaagtgat gggtttgctt ttatgccagc
     1801 aattcgaaag aaaacaggcg agacaatttt ttgtggaatg tatatgtatg acatggaatc
     1861 agatatgttg agctattggg atatgccgga ttggaattgg aaagagatcg tcaaaacaga
     1921 tagcgagact ttatctgact atttcacttt caataaaggc acaggcgaaa tcaccttgaa
     1981 acaagctgca ctcatcgcag atgccaatct ctatagcgct ggagcgcctg tgctcagtgc
     2041 gggagtcacc tatgagtggg atatctttgg atggtggtca gatgggaacg atccaggaaa
     2101 ctcagaaaat gtagatcctc cctatttttt caaagaggag acagcacatg attcccggta
     2161 tagatcaata tactcggaat cctattcatt ctctgattcc tacgaggatg gaatggatgc
     2221 gctcaacgga tggtttgagc ttattgctga gtgaggagga ctataatgaa aaagaacata
     2281 tataggtcta tagcgagagg agccttgctt gtatcattgc tcctaatttt gagcggatgt
     2341 aattttttcc caaagcaggg ctcccagctg caaaaagcgc cggatttcta tcctgagtac
     2401 agcatgttcc aaccaaaaac gcttaaggaa tttgataatg ctgtgcagct tggacatgta
     2461 agctcagaca cattgatcgc taagacgagc attgatttcg ataaggatca attcttgaaa
     2521 tgcgagcttg aggtaattgg ctcttttgag gttgaaggaa atcgctactt taggctgcac
     2581 gcttctcaga atatcttcaa gaaagccaag agaatcgaaa aactaaaagg tgtgctctat
     2641 gtcgagcatg agctccgctc ctatatcccc aagaatgagc aagggccgtt tatagacagc
     2701 attgcatcat caaagggcgc aggtgagata tccgctgtgc tgaatgatcc cgagacttgg
     2761 ggaagatatg ggcactttga aatcactcat gcaatagatg cttacagaga ccttggattt
     2821 ggcgacaata ctgtgtatgt ggtcgatatc gatactggga ttgaccaaac gcatgaggat
     2881 ttccatctaa gttcggaaga gagcataatc gagtatgcga agagcgcgtt cgatgagtat
     2941 ggcacttatg tagatgatat gaatcctttt atagaagttc ctgcgaatga gaactgggat
     3001 gatatggggc acggtaccca tactgcgggc ataattgcag cccttggtaa caatggcata
     3061 ggtgttgccg gtgtcgcatg gaaaaacgtg aaacttattt cttataaatg tttttctgaa
     3121 gtatcaccat attctggtta tgactggcct gtgtacgggg gttttgcaga cctggtagat
     3181 tggaagaaag ccaatgcgcc tgagcaaacc atccccgtca atatgtcgct cggcggttat
     3241 tacgcaagcg cttttgagct tgaaatgatc agctatgctc taaggaatgg aattgtgatc
     3301 attgcatcta tggggaacga tggcacggag agagcccaat atcctgcggc atatgcgggg
     3361 gttatcgcgg taggagccac aagggcaaac gaggaaaagg tcggcttttc tacatctggc
     3421 aaacatatat ctgtctctgc gccaggctac aatatctact ctacattcaa tgatggaggc
     3481 tatgcagaca tgtcagggac ctcgatgtcc gcgccttttg tgactggcct tgttgcttac
     3541 atgcttactt tcaatccaga tttgacagct gatcaaataa aggccattct agaacagaca
     3601 gcaatggata ttgggccaac aggttgggac accggaactg gttatgggct tgtagatgta
     3661 aggaaagctg taaccaaagt aaagaataat gaaataccat tagttggttc aatctatgct
     3721 tctggggaag ccgtgatcag tgttcaaaac ctcgatagcg aatatgattc tggcaatacc
     3781 cattatcctg atgcggtagg cggcgtgcct gtctatctct atggagcgga tggctccttt
     3841 gtagctgttg gtgtatccaa ctatactgat ggttcagtgt cattcaggct tcttaagcca
     3901 ggtgactaca ctgcaaagac aaattgggga ggtgtcccga agagtcaagc atttaacatg
     3961 cccgctactg gcaataagac ccaaagcatc aattacgaca tgaagattta ttacgcccag
     4021 actctctgga atgagcttat ggatcctgat tattttagtt gggccgacag cgtacttacg
     4081 ctctatactg aggagggttc gttgattgta gggccatatg attattcgta cctggatact
     4141 ctcatgattc ctgtgaagtc aggacagtca tatgtccttg gtatcgagcc atatttcaat
     4201 gacgaatata gttggggaga atatggttta ataataagcc aggatctgta tgattctgtg
     4261 aataccaata atggccgggg ctctggtgac gatgatacct ttgagcccaa caacgatttc
     4321 gcttcagcca aagatattga tgttgatata gaatatggtc tttacctggg agatggagac
     4381 tactttagat tcatcatacc tgaatgagag caaaatcaaa agggcacctc gaagatgggg
     4441 tgctcttttt ttgagattga agcaagatgc ctttttgcac gcgtcatttc agtgatgata
     4501 gatcggaaag cacacagaaa ttatgacgga tgcaatctta gaaaatgttg ctctctgtgt
     4561 aagcataaag cgcggcggct atttgggaga ttgctcctta aagtggagcc ttcctgcata
     4621 gcagcttttg ggctctggtg tggcctcgca tggggtggtg ttagtggtag cggaatttgg
     4681 gatctattcc tcgcatttat tattggtgcg attgcaataa gcatttcgat aggtcatttg
     4741 aagctatcgg ataagaatgg cagaagagag cttttgctct ggagcgctct atccatgggc
     4801 ctcgcgctca gcgcaggcgt gcttcg
//
LOCUS       DAOMZL010000107         4786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_16803_length_4786_cov_13.159797, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000107 DAOMZL010000000
VERSION     DAOMZL010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16803_length_4786_cov_13.159797"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /gene="rpsH"
                     /locus_tag="PLQ30_07405"
     CDS             <1..344
                     /gene="rpsH"
                     /locus_tag="PLQ30_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPW02311.1"
                     /translation="ARHEKVDIPTSKLKLEIVKILKTEGYIKNFKKIQNEGQNFIRIF
                     LKYDEKNDPIIHGLSKVSKPGRRVYSGYREMPRILNGYGIAIVSTSDGVITGRKAVEK
                     QVGGELICTVW"
     gene            358..900
                     /gene="rplF"
                     /locus_tag="PLQ30_07410"
     CDS             358..900
                     /gene="rplF"
                     /locus_tag="PLQ30_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPW02312.1"
                     /translation="MSRIGMRPVAIPKGVKVTIEPTVFHVEGPKGKLSQEYCSDIKIE
                     IRDGFAVVERKDDSKQSKAYHGLYRSLLDNMITGVSQGFKRSLVISGVGYRAEVQGNF
                     LMLSIGFTNDMIVVIPEGLRVSVDQNGQRIIIEGIDYAKVGKFASEVRSLRKPEPYKG
                     KGIRYEEERIRRKVGKTGVK"
     gene            911..1276
                     /gene="rplR"
                     /locus_tag="PLQ30_07415"
     CDS             911..1276
                     /gene="rplR"
                     /locus_tag="PLQ30_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPW02313.1"
                     /translation="MSVKELTDKRRKREKRKTHIRKSIFGAPDRPRMTVYRSNLHLYV
                     QVIDDMVGHTLSSVSTMEKQFRDLRPNVQSGNLIGEEIGKRLMEKGITQVVFDRNGYK
                     YHGVVKAIADGVRKAGVKF"
     gene            1316..1798
                     /gene="rpsE"
                     /locus_tag="PLQ30_07420"
     CDS             1316..1798
                     /gene="rpsE"
                     /locus_tag="PLQ30_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPW02314.1"
                     /translation="MQEGYTEKLIQLNRTAKVVKGGRRFSFSALSVVGDKQGHVGFGF
                     GKANDVTEAIRKSVDRAKHAMVTVPVKNGTLPHEVTGIYKATKVYMKPACPGSGVIAG
                     GPVRAILECAGYIDIISKCIGSRTAVNVVRAVFDGLDKLLDAQEVAKNRGKSLKDMWS
                     "
     gene            1801..1983
                     /gene="rpmD"
                     /locus_tag="PLQ30_07425"
     CDS             1801..1983
                     /gene="rpmD"
                     /locus_tag="PLQ30_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPW02315.1"
                     /translation="MPKIQITLVRSTIGQKPKARETVRCLGLRKIGSSTIQEASPQIL
                     GMVDKVKHLVEIKEVE"
     gene            1983..2456
                     /gene="rplO"
                     /locus_tag="PLQ30_07430"
     CDS             1983..2456
                     /gene="rplO"
                     /locus_tag="PLQ30_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPW02316.1"
                     /translation="MPDFYLHAPKGANKKKRIIGRGNSSGSGGTAGRGNKGQQSRSGG
                     KTYPGFEGGQMPLYRRLPGRGFSNKPFRKEYQIVNLCDLEAKFESGDSVDTLSLFMKG
                     LVKKPELPVKILGKGEIDIALMVSVDAVSSGAKAKIEAAGGKVETPIATGSSADA"
     gene            2474..3796
                     /gene="secY"
                     /locus_tag="PLQ30_07435"
     CDS             2474..3796
                     /gene="secY"
                     /locus_tag="PLQ30_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPW02317.1"
                     /translation="MANTLVDIFRVKELKDRILFSLLWLAIFRLGTYLPIPGINASAL
                     QSYFAQQSSTGSGGITDYLNFFAGGAFKNFSLFMMGVMPYISAQIIMQLLIVIFPKLK
                     AIAEEEGGRRKISRWTKIGTIVVAIIQSFSVTVWADRIPNAIVMSNRTLYTIIAILTV
                     TTGTMFLVWIGEQITKRGIGNGISLLIFAGIVARLPNASYELIKSIQRGEINAVYAIV
                     VLAMFVAVVGLVVIEQQGERKIPVNYAKRIVGRRMYGAQNTYIPFKINPSGVIPVIFA
                     SSLLTFPLQIAQSFGVQAKWLRDFSYWLRPSGFWYNLFYVIFIVFFAYFYTQVTLNPQ
                     EISKNIRENGGSIPGIRSDKMEEYLTRVLNRIILPGALYLALIALIPTLVQQMFNFPS
                     ELAYLLGGTSLLILVGVDLDTMAQVEAILKMHHYDGLVRKGRIKSRNL"
     gene            3849..3962
                     /gene="rpmJ"
                     /locus_tag="PLQ30_07440"
     CDS             3849..3962
                     /gene="rpmJ"
                     /locus_tag="PLQ30_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPW02318.1"
                     /translation="MKVRTSVKKICDKCKIIRRNGVVRIVCSNPKHKQKQG"
     gene            3979..4350
                     /gene="rpsM"
                     /locus_tag="PLQ30_07445"
     CDS             3979..4350
                     /gene="rpsM"
                     /locus_tag="PLQ30_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPW02319.1"
                     /translation="MARIAGVDLPNKHVDIALTYIYGIGRASAQKICETTGIDANKKI
                     NELSNEEIAELRKVIENDYKVEGRLRTEVALNIKRLMDIGCYRGLRHRRGLPVRGQRT
                     RTNSRTRKGKRKTVANKKKAV"
     gene            4360..4758
                     /gene="rpsK"
                     /locus_tag="PLQ30_07450"
     CDS             4360..4758
                     /gene="rpsK"
                     /locus_tag="PLQ30_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPW02320.1"
                     /translation="MNVAATTTKTKKRKEKKSVYEGNVYIQATFNNTIVTVTDLNGNA
                     IAWSSSGSHEFRGAKKSTPFAAQTVTEAAIQKAMQAGLREAHVYVKGPGIGRESAIRQ
                     LGVMGLKVKSINDVTPIPHNGCRPRKARRI"
BASE COUNT         1366 a         1018 c         1274 g         1128 t
ORIGIN      
        1 aggctcggca tgagaaagtc gacatcccga cttccaagct caagcttgag atcgtaaaga
       61 ttctcaaaac cgagggctat atcaaaaatt tcaagaagat ccagaatgag gggcagaatt
      121 ttatcaggat ctttctcaaa tacgatgaga agaatgaccc cataatccat ggattgagca
      181 aggtctctaa gccagggcga cgcgtctatt ccgggtatcg tgaaatgccg cggattctca
      241 atggctatgg tattgccatc gtctcgactt ccgatggtgt cataaccggt agaaaggctg
      301 tggaaaagca ggtaggcggc gagctcatct gtacggtctg gtaaggcgag gtaaagtatg
      361 tcgagaattg gaatgcgacc tgtagcaatt cccaaaggtg taaaggtgac tatagagccg
      421 acggtttttc acgtcgaagg accgaaaggg aagctctctc aggaatactg ctccgatatc
      481 aagatcgaaa taagagatgg atttgccgta gtggagcgca aggatgattc caagcagtcc
      541 aaggcttatc atggtctata tcgcagcttg ctggacaaca tgataaccgg tgtaagccag
      601 ggctttaagc gatctctcgt gatctctggt gtaggatatc gagcagaagt gcaaggcaac
      661 ttccttatgc tcagtatcgg atttacgaat gatatgattg tggtgattcc cgaaggctta
      721 agagtctcag tcgatcagaa tggccagaga ataataatcg aaggtataga ctatgccaag
      781 gttggcaagt ttgcaagcga agtgcgatcg cttcggaagc ccgaaccata taaaggcaaa
      841 ggaattcgct atgaagaaga gcgaatccgc agaaaagtcg gcaagacagg cgtgaagtga
      901 ggcagatagg atgagcgtaa aagaactgac agataagaga cgcaagcgtg aaaaacgcaa
      961 gacccatatt cgaaagagca tttttggggc tcccgatagg cctcgaatga cggtgtatcg
     1021 ttcgaatctg catctctatg tgcaggttat cgacgatatg gttggtcata ccctttcgag
     1081 cgtctctacg atggaaaagc aatttcgcga cctcagacct aatgtccaga gcggtaacct
     1141 gataggtgaa gaaattggta aaaggctcat ggaaaaaggt attacacagg ttgtcttcga
     1201 tagaaacggc tataaatacc acggtgtcgt gaaagcgatt gccgacggcg tcagaaaagc
     1261 tggcgtcaaa ttctaggggg cggcagtgga aagaacaagg aaagatgatt ccggtatgca
     1321 ggaaggctac accgaaaagc ttattcagct gaacaggact gccaaggttg tcaaaggagg
     1381 acgaaggttt tccttctcag cactgtcggt ggtcggcgac aagcaaggcc atgtcgggtt
     1441 cggttttggc aaggcaaatg atgtcaccga agcaatcagg aaaagcgtcg atagagcaaa
     1501 gcatgccatg gttacggtgc ccgtcaagaa cggcacgctg ccccacgagg ttaccggcat
     1561 ctacaaggcg accaaggtat atatgaaacc agcctgcccc ggctccggtg ttattgctgg
     1621 aggccctgtg cgtgctattc tggagtgtgc aggctacatc gatatcatct cgaagtgtat
     1681 aggttcgaga acagccgtaa atgttgtgcg tgcggtattc gatggcctgg ataaactctt
     1741 ggatgcgcaa gaagtcgcga aaaaccgagg aaagtcgctc aaagacatgt ggagttaatc
     1801 atgcccaaaa tacagataac attggttcgc agcacaattg gtcaaaagcc taaagcgcga
     1861 gaaactgtcc gctgtctggg gctgcggaag ataggttcga gcaccataca ggaagccagt
     1921 cctcagatcc taggcatggt ggataaagtg aaacatctgg ttgagattaa ggaggtggag
     1981 tgatgcctga tttctatctc cacgccccaa agggtgcgaa caaaaaaaag cgcattattg
     2041 gtagaggcaa cagttcgggt tctggtggta cggcaggtcg tggcaacaaa ggccagcagt
     2101 cgcgttctgg cggcaagacc taccctggtt ttgaaggtgg acagatgcct ctttacaggc
     2161 gtctgcctgg tcgaggcttc tcaaacaagc cattccgcaa agaatatcag attgtaaatc
     2221 tgtgcgacct agaagccaaa ttcgagagcg gagatagcgt ggataccctg agccttttca
     2281 tgaaagggct cgtgaaaaag cctgagttgc cagtaaagat attgggcaaa ggggagatcg
     2341 atatcgcgct catggtgagc gtcgatgcgg tctcttcggg tgcaaaggca aagatagaag
     2401 ccgctggtgg caaagtggag acacctatag ccacaggttc ttcggcagat gcatgatatg
     2461 aaggcgggag cgaatggcta atacactcgt cgatatcttc agggtcaagg aattaaagga
     2521 tagaattctc ttttccctgc tatggctcgc gattttccgc ctgggtacat acttgcccat
     2581 tcccggaatc aatgcaagcg cgctgcaatc atatttcgcg cagcaatcca gcactggtag
     2641 cggcggtatc accgattacc taaatttctt tgctggtggc gcgttcaaga atttttcgct
     2701 ctttatgatg ggcgtaatgc cttatatcag cgcacagata atcatgcagc tattgatcgt
     2761 catttttccc aagctgaaag caatagccga ggaagaaggt ggtcgtcgga agatttctcg
     2821 ctggacaaag attggaacga tcgtagttgc aatcattcag agtttctcgg tgaccgtatg
     2881 ggcagatcgc atacccaatg caatcgtcat gtccaaccgt acgctctata cgataatagc
     2941 catactcaca gttacgacag gcactatgtt ccttgtttgg ataggcgaac agataaccaa
     3001 gcgcggcatc ggaaatggaa tctctctttt gatttttgct ggtattgtgg caaggcttcc
     3061 aaacgcttcc tacgaactca taaaaagcat tcagcgggga gaaattaacg cagtatacgc
     3121 gatagtagtg ttggcaatgt tcgttgcggt tgtggggctc gtagtcatcg aacagcaagg
     3181 agaaagaaag atacctgtaa attatgctaa gcgaattgtc ggacgcagaa tgtatggggc
     3241 tcagaacacc tatatcccat tcaagatcaa tccttcgggc gtaattccag tcatctttgc
     3301 ttcgagtctt cttacttttc ccttgcagat tgcacagagt ttcggtgttc aggcaaagtg
     3361 gctcagggat ttctcgtatt ggcttagacc tagtgggttt tggtataatt tattctatgt
     3421 catttttatc gtcttctttg catatttcta tacacaggtt acactgaacc cgcaggagat
     3481 ctccaagaat atccgcgaaa acggaggatc tattccgggc attcgaagcg ataaaatgga
     3541 agaatacctt accagggttt tgaatcgcat catacttccc ggagcgctgt atctagccct
     3601 aatcgcgctt atcccgacgc tggtgcaaca gatgttcaac ttcccgagtg agctggctta
     3661 tctgcttggt ggtacttcct tgctcattct tgtgggagtc gatctcgata ccatggcaca
     3721 ggttgaggct atcctcaaga tgcaccatta tgacggtctc gtacgcaaag gccgtatcaa
     3781 gtcgaggaac ctctagaaat ttttgcgcga ataggattta attgcgtgtt ttgtaatagg
     3841 gggaacccat gaaggttaga acaagcgtca agaaaatttg tgataaatgc aagataatac
     3901 ggcgcaatgg tgtggttagg attgtctgca gtaatcctaa acataaacag aaacaaggct
     3961 aagagcagga ggaagctcat ggcaagaatt gcgggagtcg atctccctaa taaacatgtc
     4021 gatattgctc ttacgtatat ctatggtatt gggcgggcaa gtgctcaaaa gatttgcgaa
     4081 accacaggca tcgatgccaa taagaaaatc aatgagcttt ccaatgaaga gattgctgag
     4141 cttcgcaaag tcatagaaaa cgactataag gtcgagggtc gtctaagaac cgaagtagct
     4201 ttgaatatca agcgcctcat ggatattggc tgctatcgtg gcctacgcca tcgcagaggt
     4261 cttccggttc gcggtcagcg taccaggacg aactcgcgta cgcggaaagg caagagaaag
     4321 accgttgcaa acaagaagaa agctgtttaa cagaggagca tgaacgtggc tgcgactacg
     4381 acaaaaacta agaagagaaa agaaaaaaag agtgtctatg aagggaacgt atatattcag
     4441 gcgactttca acaatacaat tgtaaccgtg acagacctca acggcaacgc cattgcatgg
     4501 tcgagctccg gaagccacga attccgtggt gcaaagaaat ctacaccttt tgccgcacag
     4561 accgtgaccg aagccgccat acagaaggct atgcaagctg gtctgcgaga agcgcatgtc
     4621 tatgtcaaag gacctggaat tggacgcgaa tccgcgatta gacagctcgg tgttatgggg
     4681 ctcaaggtaa agtcgatcaa tgatgttacg ccgattcctc ataacggctg taggcctcgc
     4741 aaggctcgcc gcatctagga gggggttaca aaaatggctc gatata
//
LOCUS       DAOMZL010000108         4758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_16926_length_4758_cov_19.318733, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000108 DAOMZL010000000
VERSION     DAOMZL010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16926_length_4758_cov_19.318733"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLQ30_07455"
     CDS             <1..245
                     /locus_tag="PLQ30_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPW02321.1"
                     /translation="LAILAFRGQWDNLIWPLLIIQKEELKTIPLYIVLFTSELSTDEG
                     AMMAVAVIAALPVFLLFFTMSKYFLSGAGVYTGSKT"
     gene            242..892
                     /locus_tag="PLQ30_07460"
     CDS             242..892
                     /locus_tag="PLQ30_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPW02322.1"
                     /translation="MIDLYIFDEGGVLIRNHMIMGDVAAALGLDMATFLDFIAPDIKS
                     LSRGSIDSAEFWRRFSDRTGIRPSENYLKTLFRPLPDEDTFSLVDELSKKARVVCGTN
                     TIDSHHEINIQLGLYSPFHAVYASHIIGHIKPEPEFWQHILEAEGVPAERTFFTDDSE
                     ENIKAASSLGIQAVHYIDADHLRQALFELGAPIDRLFGEIGAPVHKADAPIVAPPS"
     gene            994..1941
                     /locus_tag="PLQ30_07465"
     CDS             994..1941
                     /locus_tag="PLQ30_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HPW02323.1"
                     /translation="MDNQWIFGIDGGGTSTRLRIESLEGKMLYQAESLSMNPRSVGWN
                     GSRKTLDDLFSGMYKSINLSADNCISGFAGVAGIDREADTGMMRSIIREAGNLGFDTL
                     IEVKNDSIPALAGAFGELRGILLIAGTGSIAVGADSVGRIIRSGGWGHILGDEGSAYW
                     VGLRALNAAIRFQDRRGPQTDLLGRALAYFGEKEAFSLIPAIYEDFDKAKIAAFAKIV
                     AEEQDRGDETARQIFIEAAEELALLATSIAIRLGEATIGGKIAFSGGFITNNERLWRD
                     TEARILSTLPRHRVVVPQADATTGACILARSIANQSRLY"
     gene            complement(1949..2815)
                     /locus_tag="PLQ30_07470"
     CDS             complement(1949..2815)
                     /locus_tag="PLQ30_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPW02324.1"
                     /translation="METKRFKASFFGVIVAVIWGLTFLSIKVALREFGPMSLPLFRFV
                     IASLLLALIMILTRTRFRIAWKDVPMLSLSAFSGVTLYYYFENNGIMRLTASESSLIV
                     GTIPVVTLLAEMIAFKIRPRRPVMLGIVLSFVGVALIVMRSENASTSMSGYFFMLGAV
                     LSWVAYGFMTKPLSGRYPMLTITFWQMVIGALGCMPFALAEQQSWTGFSTSVLLNATF
                     LAVFGSAIGYWLYIIVLDELGPGRSSVFINLIPVVSVTASFVFLGERLSAMQVAGACL
                     AIAGVYLASSGS"
     gene            2852..4471
                     /gene="hutH"
                     /locus_tag="PLQ30_07475"
     CDS             2852..4471
                     /gene="hutH"
                     /locus_tag="PLQ30_07475"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HPW02325.1"
                     /translation="MGGTSYVIIKNMNEVIIDGCSLSISDVVAVARHKLPVRLDPDAE
                     LRMQASREVVEFAVKKKQVKYGITTGFGKFCDVIISEEDNAQLQKNLIMSHACGQGEP
                     FAPEIVRAMMVLRANALAVGNSGVRPMIVHRILQLINEDIVPIVPEKGSLGASGDLAP
                     LAHIALVLIGMGEALYKGTRMAGALALAEAHVQPLVLEAKEGLALINGTQAMAALGVL
                     SVYDALFLYRSATIASALSFQALRGITEALDPRIHELRRQKAQIEVAQDLRRLLEDSR
                     LTSMASGTRVQDAYALRCIPQVHGACLAAIRHVWEIVSAELNAVTDNPLIFPYVLREG
                     GDIISGGNFHGEPLALALDYLGIGASELANLSERRLERMVNPTLSEGLPAFLTINGGL
                     NSGFMIVQYSAASLVSENKVLSHPASVDSIPSSANQEDHVSMGTIAARKARQIIDNAR
                     RVIAMELLAACQALDLRAIQLGFPSIVDSLSPATAQIYTIIRTVVRFSEQDRIMYPDI
                     DAVVRLLSDEILPQTMERWSWNPEPVVAHEQ"
     gene            4526..>4758
                     /locus_tag="PLQ30_07480"
     CDS             4526..>4758
                     /locus_tag="PLQ30_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02326.1"
                     /translation="MVTKRDYGLRLDRTSPQERARLISYINIKLKSLGLPVYSKEGTG
                     FVQLAADMLESFRQKNRLLPRILPPADQRIQNF"
BASE COUNT         1314 a         1172 c         1127 g         1145 t
ORIGIN      
        1 cattggcaat tcttgccttc cgcggacagt gggataattt gatttggcct cttctaataa
       61 tacaaaagga agaactcaag accatacctc tctatatagt actcttcaca tcagagctta
      121 gcaccgacga gggcgccatg atggccgtag cggtcattgc ggcattgcct gtttttctac
      181 ttttctttac catgtccaag tatttcttga gcggcgctgg agtctatacc gggagcaaaa
      241 catgatcgac ctctatattt tcgacgaagg aggggttcta atacggaacc atatgattat
      301 gggcgatgta gcggctgccc tgggattgga tatggccact ttcctcgatt ttattgcacc
      361 tgatataaaa tcactctcaa gagggtcgat agacagcgcc gagttctggc gaaggttttc
      421 tgatcgcaca gggatcaggc cttccgagaa ttatctaaag acgttattca gaccgctccc
      481 cgatgaagac actttctcat tagtcgatga attgtcaaag aaagccaggg tagtatgcgg
      541 aactaacaca atcgatagtc accacgaaat caatatccag cttggattat atagtccatt
      601 ccatgcggta tacgcatctc atattatagg tcacataaaa cctgagccag agttctggca
      661 acatattctc gaagcggaag gagtacctgc cgagcgcact ttctttaccg acgatagcga
      721 agagaatata aaggccgcct ctagtctcgg cattcaagcc gttcactata tcgatgcaga
      781 ccatctaaga caagcgcttt ttgagcttgg agcgcctatc gatcgacttt tcggtgaaat
      841 cggtgcgcct gtccataagg ccgatgcgcc tatcgttgcg cctccatctt gaattatctg
      901 aaattcctta ttgaatgcat tagctcctgc aattagaata gtctagtctt gataatgccc
      961 tagcataaaa gaaaaaaact tgcgaggtcc ctgatggata accaatggat ttttggcata
     1021 gatggcggag gtacatcgac aagactgagg atagaaagcc tggaaggcaa gatgctgtac
     1081 caggccgaaa gtctcagcat gaaccccaga tcagtcggat ggaacggctc cagaaaaacg
     1141 ctcgatgatc ttttttcagg aatgtacaag agtatcaatc tctccgccga caactgcatt
     1201 tcaggttttg ctggtgtagc tggcatcgac agagaagcag atacaggcat gatgcgaagc
     1261 atcattcgag aagccggaaa tctgggtttc gatactctca tagaagtaaa gaacgattct
     1321 atccccgccc ttgctggagc cttcggagaa cttcgaggga ttctcctcat cgcgggaacc
     1381 ggatctattg cggtgggagc cgatagtgta ggtcgaatca tccgatccgg aggatggggt
     1441 catatccttg gagatgaggg atcggcttac tgggtcgggc ttcgcgctct aaatgcagca
     1501 attcgattcc aggatagaag ggggcctcaa accgatttgc ttggcagagc ccttgcctat
     1561 tttggcgaaa aagaggcctt ttcccttatt ccagccatct acgaggattt cgacaaggcg
     1621 aaaatcgcgg cgtttgcaaa gattgtggca gaagaacaag atcgaggcga cgaaacagca
     1681 aggcaaatat tcatcgaagc cgctgaagaa ctagccttgc tcgcaacttc cattgctatc
     1741 cgactcggag aagcgaccat cggcggaaag atagcctttt caggaggttt tatcacgaac
     1801 aatgaacgcc tatggcgcga tacggaagcg cgaatactct ccacattgcc acgccaccgc
     1861 gttgttgtgc ctcaagccga cgcaacaacc ggtgcctgca tcttggcgcg ctcaatagca
     1921 aatcaaagcc gcctttacta gatcttgctt atgagccact ggatgccaaa tatacacccg
     1981 caattgcaag gcaggctccc gcaacctgca tggcgctgag gcgctctcct agaaaaacaa
     2041 aggaagcagt cacagatact accggaatga gattgataaa cacgctcgat cttcccggcc
     2101 ctagctcatc cagcacgatt atatagagcc aataacctat agccgagcca aacacagcca
     2161 gaaatgtagc gtttaggagt actgaagttg aaaacccagt ccaggattgc tgttcggcaa
     2221 gcgcaaaagg catgcaaccc agggcaccta tcaccatctg ccagaacgtt attgtcaaca
     2281 taggatagcg cccggatagc ggcttagtca taaatccata ggccacccaa gaaagaactg
     2341 ctcctagcat aaaaaaataa ccagacatag atgtcgaagc attctccgat cgcatcacga
     2401 ttagcgctac gcctacaaaa gagagtacta ttcccagcat taccgggcga cgaggccgaa
     2461 tcttgaatgc aatcatctcc gcaagaagcg tgacaacagg aatagtccct actatgagcg
     2521 acgattctga cgcagtgagc cgcattatgc cattgttctc gaagtagtaa tacaaagtta
     2581 caccggaaaa agcgctcagt gagagcatcg gaacatcttt ccatgcaatt cgaaacctgg
     2641 tccgggtcaa tatcattatc aaggccagca aaagcgaagc aattacaaat cgaaatagag
     2701 gaagactcat gggcccaaat tcgcgcagag ctactttgat cgaaagaaat gtcaaacccc
     2761 aaattacagc aacaataacg ccaaaaaacg atgctttgaa ccttttggtt tccatgacga
     2821 accatgctat cggtataggc aatctctgtc catgggtggt acttcttatg ttataatcaa
     2881 aaacatgaat gaagtgatca tcgatggttg ttcgctttct atttccgatg ttgttgctgt
     2941 tgctcgccac aaacttccgg ttcgactcga tccggatgcc gaattgcgta tgcaagcatc
     3001 cagagaagtt gtggagtttg cagtcaagaa aaaacaagta aaatatggca taacaactgg
     3061 ttttggcaaa ttttgcgatg tcattatttc tgaagaagac aatgcgcagc ttcaaaaaaa
     3121 cttgataatg agccatgctt gcggccaagg cgaaccattt gcgcctgaga tagtgagagc
     3181 catgatggtg cttagggcaa acgctcttgc ggtggggaat agcggtgtaa gaccaatgat
     3241 cgtacaccgc atacttcagc tcataaacga agatatcgtg cctattgtgc ccgaaaaagg
     3301 ctctcttggc gcttccggag acttagctcc gctggcgcac attgcactcg tgctaatcgg
     3361 aatgggagaa gccctttata aaggcacaag gatggctggg gctcttgctt tagcagaagc
     3421 ccacgtgcaa ccactcgtcc tggaagcaaa agaaggactt gcccttatca atggaaccca
     3481 ggctatggcc gcgttgggtg tactatctgt ctacgatgcc ctctttctat accgaagtgc
     3541 aacgatcgcc tccgcccttt cttttcaggc cttgcgcggt ataacagagg cgctcgatcc
     3601 gcgtatccat gaactcaggc gccaaaaagc ccaaatcgag gtcgctcaag acctgcgcag
     3661 actcctggag gattcacgac ttaccagcat ggcttcagga actagagttc aagatgccta
     3721 tgcccttaga tgcattcccc aagttcatgg tgcctgcctt gcggccatcc ggcatgtatg
     3781 ggaaatagtc tcggctgagc taaacgcagt gacggacaat cctctaatct ttccctatgt
     3841 gctccgagaa gggggcgata ttatttccgg tggtaacttc catggcgaac ccttggcctt
     3901 ggccctggat tatctaggca ttggcgcttc ggaattagct aatctctcag aacgacgcct
     3961 cgagagaatg gtaaatccga ccctctccga aggtctgcct gcttttctga ccataaatgg
     4021 cggacttaat tccggcttta tgattgtgca atacagcgca gcaagcctgg tatccgagaa
     4081 caaagtactc tctcaccctg caagcgtaga ctctattcct tcgagcgcaa atcaggaaga
     4141 tcatgtatcc atgggaacta tcgcagctcg caaggcacga cagatcatcg acaatgccag
     4201 gcgagtaatc gccatggagt tacttgcagc ttgccaagct ctggatctgc gcgccattca
     4261 gctcggtttt ccttctatcg tagattcgct atctccagcc acggctcaaa tatataccat
     4321 aattagaacc gtggttcgtt tttctgaaca ggatagaata atgtatccgg atatcgatgc
     4381 agtggtaaga ctcctcagcg atgagatcct gccccaaacc atggaaagat ggagctggaa
     4441 tcccgaacca gttgtcgccc atgaacaatg agtctaaaat ccgtctgccc cttttatttg
     4501 gagctttttt caaggagtat tgcttatggt gacgaagcgc gattatggtc tacgcctgga
     4561 cagaacctct ccacaagaga gagcccgcct tatttcttat ataaatatca agttgaaaag
     4621 tttgggcctt ccggtatatt ccaaagaagg aaccggattt gtgcaactgg ccgcggacat
     4681 gctggagagt ttcaggcaga aaaacaggct gttgcctaga atccttccac cagctgatca
     4741 gcgcatccaa aacttcat
//
LOCUS       DAOMZL010000109         4753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_16945_length_4753_cov_15.060238, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000109 DAOMZL010000000
VERSION     DAOMZL010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16945_length_4753_cov_15.060238"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="PLQ30_07485"
     CDS             <1..875
                     /locus_tag="PLQ30_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW02327.1"
                     /translation="GANASPAAYKQMREDMGLNSPFIVQYARFLFGYKHPIFGYKGLL
                     FGDLGRSYVSNRIIFELIFSSFPNTLILAVGALILAIILGIPIGIISATKPYSVADMV
                     FTVFALLGASMPVFWIAMVLIYTFANNLRLFPAIANPRDLLSLVLPTITLSMNSMAII
                     MRMTRSSMLDVMQQDYIRTARVKGMDEKTVVFKHALRNALIPVVTVAGLQFGSLLGGA
                     VLTEKIFSYPGLGSIMVDAIFQKDTPQILGSVCFVAITFTIINLAVDILYGFIDPRIK
                     SAFSKKKKTVRGDL"
     gene            872..1792
                     /locus_tag="PLQ30_07490"
     CDS             872..1792
                     /locus_tag="PLQ30_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW02328.1"
                     /translation="MSNQSFLRFLKRQSKNISQFWRQFSRNKLAVASLILVLIFALVA
                     IFADILYSYDFVTSNSLENALLKPGETSTVYFQEKDIEHTFILGSDNYGRDILGRLVH
                     GARISMIIGFVTVAMAVVIGGILGAIAGYKGGLFDTLIMRLTDIILSIPTILMSIAIV
                     AALGNSFFNLLIAISTSLIPGYIRIVRASIISIKDQEYVEAARVIGAGDFRIVLRHIL
                     PNITSPIIVQSTLNVGSSILSASWLSFLGLGILPPKPEWGNMLSEAREFIQYAPHLLF
                     APGIAILLTVLAYNIIGDGLRDALDPKLRR"
     gene            1803..2777
                     /locus_tag="PLQ30_07495"
     CDS             1803..2777
                     /locus_tag="PLQ30_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW02329.1"
                     /translation="MDKILEIKDLHVHYFSNRRTVKAVNDLSLDMQRGRVLGLVGETG
                     AGKTTLALSILNMIRAPQGKIISGSIRFNNTDILGMSNQELREMRGKDIAMIFQDPMT
                     ALNPVLTIGEQIAETIYKHEKISKAEAWSKSEEMLETVGIPGVRAKEYPHQFSGGMKQ
                     RVVIALSLACNPTLLLADEPTTALDVTIQAQVLDLMRGLIKTNNTSVLFITHSLGLVV
                     ENCDEVATMYAGEIVEYGTVEQVFGNPLHPYTRGLLGSLPDYSKGSPRLRPIPGLMPD
                     PTNLPKGCYFSPRCPNVMPECHEVHPQNTSIEGHLVKCHQCQENLHVE"
     gene            2767..3741
                     /locus_tag="PLQ30_07500"
     CDS             2767..3741
                     /locus_tag="PLQ30_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW02330.1"
                     /translation="MLNNQSELPILRVKNLKKYFHVARGTLHAVDDISFDIKRGGTLG
                     MVGESGCGKSTTGRLLVRLHESDGGSINYGGVEITKAPQKEMKTLRTKMQMIFQDPYS
                     SINPHHTVASIIGEPLKIYKKVRNMQEYEEKVRSLMETVGLADRLYDAYPHELDGGRR
                     QRIGIARALSLEPQFIVCDEPVSALDVSIQAQILNLLMDLQDSLNLTYLFITHDLSVV
                     KHISDEIVVMYLGQIVEKANTDELFKHPIHPYSKALESAIPIPRLNARKERIILKSEV
                     VSPIDPKPGCRFAPRCNYAVAACSESDPILREICPGRFVACHRAEMFV"
     gene            3778..>4753
                     /locus_tag="PLQ30_07505"
     CDS             3778..>4753
                     /locus_tag="PLQ30_07505"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HPW02331.1"
                     /translation="MVPIFDMHADIIMDILRREDISDENSVLLRFKQHIDRMKRGAVQ
                     GAVLVDCRMAGESAETKNFEEFIAISKILRSHQDESYLIATSSEEFDNSIRDGRWVGI
                     LCYEGLRAANGNLLWLKKLYKEAGLRIAMLTHNDSNEFATGAADPNGNPASKNPLGLT
                     EAGQKAVELMNEMGILIDLSHASPMTRRDVLSYSKKPVMLSHTSSAEVYDNGRNLSDK
                     DIREIAEHGGLIGCMTSPAALADIRDRANHTLERYMHHLRHMIDVAGEDHVGLGLHFC
                     EYLYTQEEYPPVEGLEDASKAGVILKEISKWGLSSYQIEKIAYRNFARV"
BASE COUNT         1325 a         1015 c         1100 g         1313 t
ORIGIN      
        1 tgggagcaaa tgcttcacct gcagcatata agcagatgag agaggacatg gggttgaatt
       61 caccattcat tgtacagtat gcaaggtttc tctttggata taagcaccct atctttggtt
      121 ataagggact attgtttgga gaccttggca ggtcctatgt gtcgaatagg ataatattcg
      181 agctcatttt cagctctttt cccaatacgc ttatccttgc agtcggagcg ctaattcttg
      241 ctataatact gggtattccg attggaatca tctctgcaac aaaaccatac tctgtggctg
      301 atatggtttt tacggttttt gctctcctgg gagcatcgat gccagtattc tggattgcaa
      361 tggtgctcat ctataccttt gccaacaact tacgcctttt tcctgccata gccaatccgc
      421 gggatttatt gtctcttgtg ctgccaacaa taactttgag catgaattcg atggccatca
      481 ttatgagaat gacacgttca tcaatgctcg atgtcatgca gcaggattac atcaggacag
      541 cgagagtaaa aggcatggac gaaaagacag tggtctttaa gcatgccttg cgtaatgccc
      601 tcattccagt agtcacagtc gcaggtcttc agtttggaag tcttcttggt ggagccgttc
      661 tgaccgaaaa gattttttcc taccccggac ttggttctat aatggtggat gcaatatttc
      721 aaaaagacac gccgcagatt ctcggatcgg tatgtttcgt tgcaataaca ttcaccatca
      781 taaatcttgc ggttgacatt ctatatggat tcatcgaccc acgcattaaa tccgctttct
      841 cgaaaaagaa aaaaacagtg cgaggcgacc tatgagtaac cagtcatttt tgagattctt
      901 gaagcggcaa tcaaagaata tttctcaatt ctggaggcaa ttctcaagga acaaactcgc
      961 agttgcaagc ttgattctag ttttgatatt cgcgcttgtt gctatttttg ctgacattct
     1021 ctattcgtat gattttgtta cttcgaattc gctcgagaat gcgcttctca agcccggaga
     1081 aaccagtacg gtctattttc aagagaaaga tatagagcac actttcatcc ttggctcgga
     1141 caattatggt cgtgatattc tcggcaggct ggtccatggt gcaagaatct ctatgatcat
     1201 cggttttgtt actgtagcga tggcggttgt aataggtggc attttaggag ctattgctgg
     1261 atataaggga gggttattcg atacactcat tatgcgactt accgacataa tcctttctat
     1321 tccgaccatt ctaatgtcga ttgccattgt agctgcgctt gggaatagct ttttcaattt
     1381 gctgattgct ataagcacat ccttgattcc tggctatata cgaatagtcc gcgcttctat
     1441 tatctcgatc aaagaccagg aatatgttga agcagcgcgc gtaataggtg caggagattt
     1501 tcggatcgtg ctcaggcaca tactgccaaa tatcacctct ccgatcattg ttcagtctac
     1561 gctcaatgta ggctcatcga tactctctgc ctcttggttg tcttttttag gactaggaat
     1621 tcttccgcca aaaccagaat ggggcaatat gctttccgag gctcgcgaat tcattcaata
     1681 cgcgccgcat ctcctatttg cacccggaat agccatcctg ctcaccgtat tggcctacaa
     1741 catcattggc gatggattac gcgatgctct agacccaaaa ctcaggcgtt aggaagctga
     1801 ttatggataa gattctagaa ataaaagatc tacatgtaca ctatttttca aatcgaagaa
     1861 cggtcaaggc ggttaatgat ctttcgctgg atatgcagag aggtcgagta ttgggtcttg
     1921 ttggagagac gggagcgggt aagacaacgc tagcactttc gatactcaac atgatacgtg
     1981 caccccaagg caagatcatc tccggatcca ttcgcttcaa caatacagat attctcggca
     2041 tgtccaatca agagctgcga gaaatgcggg gaaaagatat agcaatgatt tttcaggatc
     2101 ctatgaccgc tctcaatcct gtgctaacca ttggagaaca gatcgctgag acaatctata
     2161 agcatgaaaa gatatctaag gctgaggctt ggtcgaagtc tgaggagatg ctcgaaactg
     2221 tcggcatacc tggcgtcaga gcaaaggaat atccccacca gttttcaggt ggaatgaaac
     2281 agcgcgtcgt aatagccctt tcattagcat gcaatccaac gcttcttctg gcggacgagc
     2341 ctacaacagc cctggatgtc acgattcagg cgcaagttct cgatttgatg cgcggcctca
     2401 taaaaaccaa taatacatcg gttctattta ttacccattc gttagggctg gttgtcgaga
     2461 actgtgatga ggtagcgacc atgtatgcag gagaaatcgt ggagtatggc acggtagaac
     2521 aggtgtttgg gaatccgctt catccttata caagaggtct tttgggttct ctccctgatt
     2581 atagcaaagg ctctcctcgt ctccggccaa tacccgggct tatgcccgat ccaaccaatt
     2641 tgccaaaagg atgttatttc agcccgcgat gccctaatgt aatgcctgag tgtcacgagg
     2701 ttcatccgca aaacacttct atagaaggcc atttggtgaa atgccatcag tgtcaggaga
     2761 atttgcatgt tgaataatca atctgaattg cccattttgc gagtaaaaaa cctcaaaaaa
     2821 tacttccatg ttgctagagg gactctccat gcagtggatg acatttcctt cgatataaaa
     2881 aggggaggaa cacttggtat ggtcggcgaa tccggttgtg gcaaatctac tactggaaga
     2941 ttgcttgttc gccttcatga atcagacgga ggttctatca attatggcgg agttgagatt
     3001 acaaaagctc cgcaaaagga aatgaagaca ctgcgcacca aaatgcagat gattttccag
     3061 gacccatatt cctctatcaa tcctcaccat actgttgcat cgatcatcgg tgagccgctc
     3121 aagatatata aaaaggtaag aaatatgcag gaatatgagg aaaaagtgcg atcgctcatg
     3181 gaaactgtcg gtctagctga tagattgtat gatgcctatc cacatgaatt agatggaggc
     3241 agaagacagc gtatcggcat tgcgcgtgcc ctttcgcttg aacctcaatt cattgtctgc
     3301 gatgagccag tatctgccct ggatgtatcg attcaagcac agatactcaa cttgctcatg
     3361 gatcttcagg actctttaaa cctaacatat ctttttatta ctcatgatct ctcggtggta
     3421 aagcacatat ctgatgaaat tgttgtgatg taccttgggc agatcgtaga gaaagcaaac
     3481 accgatgagc tctttaagca tccaattcat ccctatagca aggctctgga atcggctatt
     3541 cctattccaa gactcaatgc cagaaaggaa cggataattc taaagagcga ggttgtgtcg
     3601 ccaatagatc caaagccagg ttgtcgcttt gcaccgcgct gtaattatgc cgtcgcagct
     3661 tgcagcgaat ctgatcctat tctcagagaa atatgtccag gtcgttttgt ggcctgccat
     3721 cgcgcagaaa tgtttgtctg aaagttagtt ctagcgctaa cagagctggt gaaatcgatg
     3781 gtacccattt ttgacatgca tgctgacatc atcatggata tattgcgaag ggaagacatc
     3841 tctgatgaga attcagtgct tttgagattc aagcagcata ttgatagaat gaaaagggga
     3901 gctgtccagg gtgctgtctt agtcgattgt cgcatggctg gagaatccgc cgaaacaaag
     3961 aacttcgaag aattcatagc gataagcaaa atattgcggt cacatcaaga tgaatcatat
     4021 ctgattgcaa cgtcttctga agaattcgat aattcaatta gagatgggcg atgggttgga
     4081 attctatgct atgagggctt gcgcgcggcg aatggcaatc ttttatggct aaaaaaatta
     4141 tataaagaag caggccttcg aattgccatg ctaactcata acgatagcaa tgaatttgcg
     4201 actggagccg ccgatccaaa cggaaacccc gcatcgaaga accctcttgg cttgacagaa
     4261 gcgggacaaa aagcagtgga gctcatgaac gagatgggaa ttctcataga tctgtcgcat
     4321 gcaagtccta tgacaaggcg agatgtccta tcgtattcga aaaagcctgt catgctgtct
     4381 cacacatcga gcgcagaagt atatgacaat ggcagaaacc tttcggataa agacataaga
     4441 gaaatagctg agcatggagg gttgatagga tgtatgacaa gcccagcagc gttggctgac
     4501 ataagagaca gagccaatca tacgcttgag aggtacatgc atcatttgcg gcatatgatc
     4561 gatgtggctg gcgaagatca tgtgggactg ggcttgcatt tctgtgaata cttatataca
     4621 caagaagaat atcctccggt cgagggcttg gaagacgcat cgaaggctgg tgtaattctc
     4681 aaggaaataa gtaaatgggg cctttcttca tatcaaatcg aaaaaatcgc atatcgcaat
     4741 tttgctaggg ttt
//
LOCUS       DAOMZL010000110         4717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_17107_length_4717_cov_14.087945, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000110 DAOMZL010000000
VERSION     DAOMZL010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17107_length_4717_cov_14.087945"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLQ30_07510"
     CDS             complement(<1..241)
                     /locus_tag="PLQ30_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02332.1"
                     /translation="MKKFVVLLMIALFVFSAQSSFACTIVGVGADATVDRSTIVTHND
                     DSTSADFRLWIIPGQEWPEGSTRDLVIDSHNYGDFG"
     gene            complement(389..1576)
                     /locus_tag="PLQ30_07515"
     CDS             complement(389..1576)
                     /locus_tag="PLQ30_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HPW02333.1"
                     /translation="MKKNNSMGEKQSFPYFKVICLVIALIFAFFGGREFYELRHFKET
                     FVSAPGLTEVKMLSDYLSSLKGTWMDTPIFVYDSGVPGGNILYMANVHPYEPATSLSA
                     YILMENINVTKGKVFVIPQGNRSGSTTGMLGNAYPKFFHVPTSFGEKTLRIGDRGTNP
                     LDQWPDPFTFVHYPSRQNLAYQDIRNMNRTYPGRENGTLTERASYAIMELIRKENIDL
                     SIDVHEASIMYPVVATYVAHERSFDIAMMAAMMLTATSFDMKCESSPKGLRGLSHREW
                     GDFSDTLAVLMETPEPFIDRVVGPMTEELFTEGKDEFLATAAEHGLTYVPYNLEFGSP
                     LWYRCGRHLSGALEVINQMNQFYPEKEVIVEWPTYEDLKEHDCGYFFHDPDKADPNFV
                     FEI"
     gene            complement(1773..3059)
                     /locus_tag="PLQ30_07520"
     CDS             complement(1773..3059)
                     /locus_tag="PLQ30_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02334.1"
                     /translation="MYLKTSIVLAAMVVSFALSSWKLKSPEISMVITAIVGALVAGLG
                     LPVRILVEGLFTNFDLAILFVCASVFINIYSYSGSINIVTRKLVQRVDNKWVLLICMA
                     VFMLIPGALTGAGSVSVFVVGGLVATVVRYMGLSDRKTAAFVFIFAILSAAAPPINIW
                     TMMMTAQANMPYVGFEKLLLIPILIVGAFTIIYLGHGTKPESKEKILAELPEAASDMG
                     WFRILCPPVVLIALILLSLYTPFSIPVLGLPLMFVIAAIAAILCNPKKVSFKEYYGVI
                     AGTIEQIFPLVATVISVGVLINVMAATGVRGLLSITFITLPMILIFFTVLLFCPFAQG
                     CLSYGSAIIFGTPLIFMFNAAGMDTTVICAALSLIFPIGDCLPPSRIVGRVSIETVGY
                     KGSYMSFLKQILLPLLVLGGLALIMLIIPNKLSFLK"
     gene            complement(3109..3714)
                     /locus_tag="PLQ30_07525"
     CDS             complement(3109..3714)
                     /locus_tag="PLQ30_07525"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HPW02335.1"
                     /translation="MRKGFFILLLVALLSCIGPFSVFAAISGDPIKQYGLPFLITNAG
                     QGPGGKMARLLVSQTKTLELDKDFFYLAEPVEADLKARPYKALMVVIGSSDKGLGASG
                     ITIDQEIARLQRIVKASKDMKLPIIAVLLEKDKRSNVATNPNERCIDTICPYAEWMIV
                     VKDGNTDKRFDKIKEKYHIPLTIIDNAIDFTSLCKQIFIKK"
     gene            complement(3878..>4717)
                     /gene="glgP"
                     /locus_tag="PLQ30_07530"
     CDS             complement(3878..>4717)
                     /gene="glgP"
                     /locus_tag="PLQ30_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPW02336.1"
                     /translation="EDYDMAMGRYMTSGSDVWLNTPRRPQEASGTSGMKAGMNGVLNC
                     SVLDGWWAEAYSSEIGWAIGSGEEYKDEALQDEIEAEDLYDLLEHEILPLFYTRGRDN
                     IPRGWLTKMRSSMKTIGSKFATHRMLKEYYTNYYEPALRESKTLEADNYRASVSLAQY
                     IEKVKRVWNGVRIVEFSDDGAPVVPRGSAIEVRALVDLAGLLPDDAAVECYLGRLSSK
                     GEILEGTRIPMTLLGPEGQYYRYQCDIRSEITGQIGYSVRVLPAHPALDGRFVPGLVR
                     WAQ"
BASE COUNT         1336 a         1158 c          979 g         1244 t
ORIGIN      
        1 tgccaaaatc tccatagtta tgcgagtcga tgacaagatc gcgagtcgac ccttccggcc
       61 attcttgccc cggaattatc caaaggcgaa aatctgccga tgtagaatcg tcgttgtgag
      121 ttactattgt cgaacgatct acagttgcat ctgcgccaac gccgacaata gtgcatgcaa
      181 acgatgactg cgcactaaat acgaataggg ctatcatcag cagcactaca aatttcttca
      241 tatcatactc ctttcttggg gaattcccca tattctcttg tctccgctta tcttttgata
      301 agttatccta tctcagatag ccgcggtata gagccaaatt ccaaatatct tcgacaccat
      361 gtcattcttc tattcttttt attttcgctt atatctcgaa tacgaaattg ggatccgctt
      421 tatcaggatc gtgaaagaag tacccacaat catgctcttt tagatcttcg taagtcggcc
      481 actcaacgat gacttccttt tccggataga actgattcat ctgattgatc acttcaagag
      541 ctcctgaaag gtgtcttcca cagcgatacc aaagaggaga accaaactcg aggttatatg
      601 gaacataagt gagtccgtgc tccgctgcgg tagctaaaaa ttcatccttc ccttcagtga
      661 acagctcctc tgtcataggc cccactactc gatcgataaa aggctccgga gtctccatga
      721 gcacggcaag agtatcgctg aaatcgcccc attcgcgatg agacaatcca cgtagacctt
      781 ttggagaaga ctcgcatttc atatcaaaac tggtagccgt aagcatcatt gctgccatca
      841 ttgcaatgtc aaacgaacgc tcgtgtgcca cataggtggc taccacagga tacataatag
      901 atgcttcgtg aacgtctata gataggtcga tattttcctt acggataagc tccattattg
      961 cataggaggc tcgctccgtg agagtaccat tttcccttcc tggataggtc cgattcatat
     1021 tgcgaatatc ctgataggcc aaattctgtc tcgatgggta atggacaaat gtaaaaggat
     1081 caggccattg atcgagagga ttcgttccac gatctccgat tcgcagcgtt ttctctccga
     1141 aagatgtcgg aacatgaaag aacttaggat aagcgttacc cagcatgcct gtagtcgatc
     1201 cacttctgtt gccctgaggt attacgaaaa ccttcccctt ggttacattg atattctcca
     1261 tcaaaatata agccgaaagg ctggtagccg gctcataagg atggacattg gccatgtaaa
     1321 gaatattgcc tccaggtaca cctgaatcgt agacaaatat cggagtatcc atccacgttc
     1381 ctttgagact cgaaaggtaa tcgctcagca tctttacctc ggtcaaacca ggagccgaga
     1441 caaaggtctc tttaaagtgc ctgagttcgt agaattccct tcctccgaag aaagcaaata
     1501 tcagtgcgat tacaaggcat atcaccttaa aatatggaaa agattgcttc tcccccatgc
     1561 tattattctt tttcataatt ccatcctctc atttgatgcc cagcacgcgc agaagaaagg
     1621 gaataatgca gtaggcatat acgatgtcat acactcggcc tgtacgtgat gtcctcttga
     1681 ataaattcct aaggaggaaa aagcctataa aagcagcaaa aatgaaatag attatatgta
     1741 ttacttgttc aagaaaaacc atttgtttct cctcatttaa ggaaactgag cttgttagga
     1801 ataatcagca tgataagtgc caaacctcca agcaccaata aaggcagcaa tatctgtttt
     1861 aggaaagaca tataagagcc tttataaccc actgtctcga tggagacacg acctacaatt
     1921 ctcgagggag gaagacaatc gccgataggg aagatgaggc ttaacgcagc acatatcact
     1981 gtagtatcca tgcctgctgc attgaacata aaaataagcg gagtaccgaa aatgatcgcg
     2041 cttccatagc tgaggcatcc ttgtgcaaag gggcaaaaca atagcacagt gaagaatatg
     2101 agaatcattg gcagcgttat aaaagtaatg ctaagaagcc cgcgcacacc ggtcgctgcc
     2161 attacgttta taagtacccc aacgcttatt accgtagcaa ccaatggaaa aatctgctcg
     2221 atggtgcccg caataacgcc gtaatactcc ttaaacgata cttttttcgg gttacataga
     2281 atagcggcga tcgcggcaat cacaaacatt agaggaagcc ccagaaccgg tatagaaaaa
     2341 ggcgtgtaca aagacagaag tattaaagct attagaacaa ctggtggaca taaaattcta
     2401 aaccagccca tgtccgatgc tgcttcagga agctccgcaa gtattttttc tttgctttca
     2461 ggctttgtac cgtggccgag gtagattatt gtaaatgcac ctacaatcag aatgggaata
     2521 agcagaagct tttcaaatcc cacatatggc atgtttgcct gcgcagtcat catcatggtc
     2581 caaatattga tgggtggagc agcggcagag agaatggcaa agatgaatac gaaagccgcg
     2641 gtctttctgt ccgaaagccc catatatcga acaaccgtag ccacaagacc gccaaccaca
     2701 aataccgaga cgctccccgc tcctgtcagc gctcccggaa ttagcataaa gacggccata
     2761 cagatcagga gtacccactt attgtctacg cgttggacta attttcttgt cactatatta
     2821 attgaccctg aatatgaata gatattgatg aatactgacg cacagacaaa caagatagcg
     2881 agatcgaaat ttgtgaacag tccttctacg agaatccgta caggcaagcc aagccccgca
     2941 accagcgcac ccacaatagc ggttatcacc atcgaaatct caggtgattt tagcttccag
     3001 ctagaaagcg cgaatgagac gaccatggca gccaaaacta ttgatgtctt aagatacata
     3061 gttatccttc tttatcaagc tgatgcccga gtttgatacg agctcttatt attttttgat
     3121 gaagatctgc ttgcagagcg aggtgaaatc tattgcatta tctatgatgg tcaatggtat
     3181 gtgatatttc tccttgatct tgtcgaagcg cttatcagta ttgccatcct ttacaacaat
     3241 catccactcg gcataagggc aaatcgtatc tatgcagcgc tcgtttgggt ttgtagcaac
     3301 gttcgaacgc ttgtctttct cgagaagcac agcgataatc ggaagcttca tatctttcga
     3361 cgcctttact attcgctgca gccttgctat ctcttgatcg atggttattc cagaagcacc
     3421 tagacctttg tccgaagatc ctatgaccac cataagagcc ttatatggtc ttgctttaag
     3481 atctgcttca accggttcgg ccaagtagaa gaaatcctta tctaattcca gcgtcttagt
     3541 ctgcgagacc aaaagacgcg ccattttgcc acccggccct tgccctgcgt ttgtgataag
     3601 gaaaggaagc ccatattgct ttataggatc accgcttatc gcagcaaaaa ccgaaaaggg
     3661 cccgatacag gacagcaaag ccaccaacaa caggataaaa aatccttttc tcatatataa
     3721 ttcctttcgt ttggtttttt gaattattgg agttacctga aaaccaaatc aaaatctgtt
     3781 agaccagtcg atttacacct ggcaatatag aattctatga gcagacttcg cttttgtcaa
     3841 cgcgatattc tcatttattc gtttttcctt aatctgccta ttgagcccag cgaacaaggc
     3901 ctggcacaaa acgaccgtct aaagcgggat gcgccggcaa aacgcggact gaataaccta
     3961 tttggcctgt tatctcgctc cttatgtcac attggtatcg atagtattgc ccctcaggtc
     4021 ccaatagggt cattggtatg cgcgttccct ccaggatttc tcccttgctc gagagtctgc
     4081 ctaagtagca ttcgaccgca gcatcatctg gcagaagacc agcaaggtcg acgagagctc
     4141 tcacttcgat ggcgcttcct cttggcacaa cgggagctcc atcatcgctg aattcgacga
     4201 tccgaactcc attccaaaca cgtttgactt tttctatgta ctgagccagg gatacgctgg
     4261 cgcgataatt atcggcctcc agtgttttac tctccctaag agcaggctca tagtagttgg
     4321 tatagtattc cttgagcatt cggtgcgttg cgaacttaga gccaatggtc ttcatcgaag
     4381 agcgcatttt cgtgagccag cctcgcggaa tgttatctcg ccctctggta taaaagagcg
     4441 gcaaaatctc atgctccaac aggtcgtaga gatcttctgc ttctatctca tcctgaagag
     4501 cttcatcctt gtattcctca ccagaaccga tagcccagcc gatctcggaa ctataggcct
     4561 cagcccacca accatccaga accgaacagt tgagtacgcc attcattcca gccttcatgc
     4621 cggaagttcc ggacgcttcc tgtggacggc gcggcgtgtt gagccaaaca tctgagccac
     4681 tcgtcatgta gcgacccatc gccatatcat agtcttc
//
LOCUS       DAOMZL010000111         4685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_17260_length_4685_cov_13.003024, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000111 DAOMZL010000000
VERSION     DAOMZL010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17260_length_4685_cov_13.003024"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..994
                     /locus_tag="PLQ30_07535"
     CDS             287..994
                     /locus_tag="PLQ30_07535"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HPW02337.1"
                     /translation="MNYSKDQQISDTLEGESIVFRYIQQQIISGNLKAGDKLPSERAL
                     SQVLNVSRGYVRKALSRLDHYGLIRTLPQRGTIVAELGSKAISGLIASIASFRDSFSP
                     IDLFDIRELLEIYAAKRAAMLATHENIEEIQKWHMEFKLKADIGQRALDEDHLLHIAI
                     AKASKNEVCLSLTSYITPQIIALNEGFPESDPDRFMNTYREHERIVNAILANDEAKAE
                     EAMRNHMHAARQRRFAE"
     gene            1042..2280
                     /locus_tag="PLQ30_07540"
     CDS             1042..2280
                     /locus_tag="PLQ30_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPW02338.1"
                     /translation="MKKRLAFSVVAVVLLTFSAAVYAQTKPVTIVHWYWADNPKYSAE
                     MQKIAADFNATNGKGITVVAQEYPWDGGAYSETLFRAVMGGGGPDTSSFKLTSTPLFT
                     ANGLLVNLDSNLEKWKDRDKIDPSLYDTMRKASGTKSVYVMPWNTQILYVYYRPSLLK
                     KAGISVPKTYAEFLEACKKLTMDTNGDGKTDVYGFGMRGAKGGQEPWGSFIWARGGNF
                     DDMTTPQAVQGMQDFIDLYKNGYAPPTAPMDGFNEIIANFKSGKTAMTIHHIGSSIGM
                     VETFGDDVDAFAFPGGVGRWTSMGDTENVILKSSKNKDAAFEWVAYLATGKGQEAWCT
                     ATGNVPVSKDVQQLPFFQNNRFMKVSIEGAPYAGIFPILDTTTEWINTIWPNTVAAAL
                     TSKISAAEAMKTLQKGLWGK"
     gene            2451..>2667
                     /locus_tag="PLQ30_07545"
     CDS             2451..>2667
                     /locus_tag="PLQ30_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPW02339.1"
                     /translation="MVFFPVLNAIAMSFQSYDLRRPSQIRFVGFANYIAVLHDGLFWQ
                     ALLKTVIWVVAGVGFQFIFGFILALLLN"
     assembly_gap    2668..2767
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2768..3421
                     /locus_tag="PLQ30_07550"
     CDS             <2768..3421
                     /locus_tag="PLQ30_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPW02340.1"
                     /translation="AGVGFQFIFGFILALLLNRKFWGRGLFRSISLIPWVTPGVLIGL
                     MWRWIYDGNYGVLNDLLMKLGLIDNYIPFLAQQSTAFPAVILTIIWQGIPFFALMLLA
                     GLQGIPDELYDAADVDGANRIQRFFWIVVPSLRNTIFVTTLLRIIWVANSVDVIFNLT
                     EGGPAYSTQTLSVYVFNKGNTLNLGYASTLAIFLTIGLSVVSIPYLKNLFKAQEEAK"
     gene            3421..4296
                     /locus_tag="PLQ30_07555"
     CDS             3421..4296
                     /locus_tag="PLQ30_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPW02341.1"
                     /translation="MELQSREAQKSFYKAPYKRKLLHALPGHILLAMMLIFLLFPFYW
                     TFVTSIKQEKELYSSTVTYWPAKVTFDSYKKLFAEYNFIKPMINSLLVAVVTTLLSLV
                     VSLLAAYAFSRYRFKGRQFFMTLFLTNNMFPTVLLLIPLFAIMRKIGILYTPSSLILS
                     YTTFTIPFSVWLLTGYLDDLPRSLEEAAMVDGANRFQAFIKIILPMLLPCVVATGAYI
                     FMTSWNEYTFAVMFTNERNRTIPVALKNFIGQLGVEWGMLTAGGVITIIPVCIMFFFA
                     QKRLVEGLTAGAVKG"
     gene            4313..>4685
                     /locus_tag="PLQ30_07560"
     CDS             4313..>4685
                     /locus_tag="PLQ30_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPW02342.1"
                     /translation="MDELLLEQKAQAIRRDIIEMLYRCQSGHPGGSLSCVEILMALYY
                     EVAHVDPTNPSWEMRDRIVLSKGHGCPALYAILADKGFFPRNDLWNLRQIGSHLQGHP
                     DMRKTPGIDVSTGSLGQGASVA"
BASE COUNT         1289 a          909 c         1032 g         1355 t
ORIGIN      
        1 cacaacaatt gacaatattt atctatttta gcatataaat cccttaccaa gaagcatttt
       61 gagagacctt tttagagcga actcaacttt aaatcctcaa aaaacagtgc acttttgatt
      121 cttaaatgac aatgctaaaa attactcttg acaattcatg aatgttggct aatactacta
      181 tatctggtta accagattgt agcccggact gcaactaggc tgcaactaaa ctgcagcgca
      241 agctgaggct gagatagaga gtaagggagg ggtaacgcgt ggcacaatga attattcaaa
      301 agatcaacag atttctgata ctcttgaagg cgaatccata gtatttcgct atattcagca
      361 acagatcatt tctggaaacc tcaaagcggg tgataaattg ccctctgaac gcgccttgag
      421 ccaggttctc aatgtgagcc gagggtatgt gcgcaaagct ctctctcgac ttgatcatta
      481 tggccttata agaacattac ctcaacgagg tacgattgtt gcagaacttg gaagtaaggc
      541 tatctctggt cttatcgcaa gcatcgcaag ctttagggat tcattttctc caatcgattt
      601 atttgacata agagagcttc ttgaaattta tgcggcgaag agggctgcga tgctggctac
      661 ccatgaaaat attgaagaga tccaaaaatg gcatatggaa ttcaaattaa aggctgacat
      721 tggccagcga gcgctcgatg aggatcacct ccttcatatt gctatcgcaa aagcgagcaa
      781 aaatgaggtg tgtctttcgc ttacaagcta tattacgcct caaataatcg cgctgaatga
      841 aggtttccca gagagcgacc cagaccgctt tatgaataca tatagagaac atgagcgaat
      901 tgtgaatgct attttggcaa acgatgaggc gaaagctgaa gaagccatga ggaatcacat
      961 gcatgcagcg cgacaaaggc gattcgctga atgacgaatc gctttgcaat aaggagtttt
     1021 aggagaagga ggttgattga catgaaaaaa agactggcat tctcagtagt ggcggttgta
     1081 ttactgacat tttcagcggc tgtgtatgca cagacaaagc cggtaacaat tgtccattgg
     1141 tattgggcgg ataatcctaa gtattccgcg gaaatgcaga aaatcgcggc ggacttcaat
     1201 gcgacgaatg gtaaaggtat tacagtcgtt gcgcaggaat atccatggga tggtggagca
     1261 tatagtgaaa ccctctttcg tgcagtgatg ggaggaggcg gaccagatac ttcttcgttc
     1321 aagctcacat cgactcctct tttcaccgca aacggcttgc ttgtcaattt ggatagcaat
     1381 ctcgagaaat ggaaagatag agataaaatt gatccaagtc tttatgatac gatgagaaaa
     1441 gcgagcggta ctaagagcgt atatgtcatg ccatggaata ctcagattct gtatgtgtat
     1501 tatcgtcctt cgctattaaa gaaagcgggg atatctgtac caaaaacata tgcagaattt
     1561 cttgaagcat gcaagaaact cactatggat accaatggag atggcaagac agatgtctat
     1621 ggatttggta tgcgaggagc aaaaggcggg caagaacctt ggggaagctt tatttgggct
     1681 cgcggtggta attttgatga catgacgact cctcaggcag ttcaaggcat gcaggatttc
     1741 atcgatctat ataaaaacgg ttatgcccct cctacagctc caatggatgg atttaatgag
     1801 atcatcgcca atttcaaatc cggcaagaca gcgatgacca tccatcatat tggctcttcg
     1861 attggaatgg ttgagacatt cggagatgat gtcgacgcat ttgcgtttcc tggcggtgtg
     1921 ggacgttgga cctcgatggg cgatactgaa aatgtgattc tcaagtcttc aaagaataaa
     1981 gatgctgctt ttgaatgggt tgcgtatctt gctacaggga aaggtcagga agcatggtgc
     2041 accgcaaccg gtaatgttcc agttagtaaa gatgtgcagc agcttccttt tttccagaac
     2101 aatcgtttta tgaaggtgtc aattgagggc gctccctatg cgggaatttt cccaattctt
     2161 gataccacaa cggaatggat aaatactatt tggccaaaca ccgttgcagc agctcttact
     2221 agtaagattt cagctgcgga agcaatgaag actctccaaa aagggctatg gggtaaatag
     2281 taaggaatgc atacctggca gctggttttt tgtcagctgc cagtacattt tcaatattta
     2341 aagtatctta ggaattcaaa gaggctttaa ttcatatcac aatgcacaaa cgaaaaacaa
     2401 atatatggcc atatttactc attatgccag ccttattggt gatatgtgcg gtggtgtttt
     2461 ttcccgtact aaatgcaatt gcgatgagct ttcaaagcta tgacctccgc cggccaagtc
     2521 aaatccgctt tgtagggttt gcaaattata tcgcagtgct tcacgatgga ttgttctggc
     2581 aagctttact taagacagtt atctgggtgg tggctggtgt tggctttcaa tttatctttg
     2641 gtttcatatt agcgctgcta ttgaatannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnngct ggtgttggct ttcaatttat ctttggtttc atattagcgc tgctattgaa
     2821 tagaaaattt tggggacgtg gactttttcg ctcaataagc cttattccat gggttacacc
     2881 aggtgtgttg ataggtttaa tgtggcgctg gatctatgat ggtaattatg gagtcctcaa
     2941 tgatctattg atgaaacttg gtctgataga caactatatt ccatttcttg cccagcaatc
     3001 caccgcattt cctgctgtaa tcttaactat catctggcag ggaatcccgt tttttgcgct
     3061 tatgctactt gctggtcttc aaggtattcc agatgaatta tatgatgctg cagatgtgga
     3121 cggggcgaat cgaattcaga gatttttttg gatcgttgta ccatcactga gaaatactat
     3181 ctttgtcaca actcttctaa ggataatttg ggttgcaaat tctgtcgatg tgattttcaa
     3241 tttgacagaa ggtgggccag cgtactctac acagacttta agcgtatatg tctttaataa
     3301 aggaaataca cttaacctcg gatatgcctc tacgcttgca atttttctga caataggcct
     3361 atctgttgtt tctattcctt atctgaagaa cctctttaag gcacaggagg aagcaaagta
     3421 atggaattac aaagcagaga agcgcaaaaa tccttctata aagcgcctta taaaagaaag
     3481 ttgctgcacg ctttgccagg acatattttg ttagcgatga tgctcatttt tctcctcttt
     3541 cctttttatt ggacctttgt gacctcaata aagcaagaaa aagaactcta ttcaagcact
     3601 gtcacctatt ggcctgcaaa ggtgactttt gactcataca aaaagctctt tgctgaatac
     3661 aattttataa aaccaatgat caatagctta ctggttgctg tcgtaacgac tctgttgtcg
     3721 cttgtcgttt ccttacttgc tgcatatgcc ttttccagat atcgatttaa gggacgacag
     3781 ttcttcatga cactttttct tacaaacaat atgttcccaa cggtattatt gcttattcct
     3841 ctttttgcta ttatgcgcaa aattggcata ctctacacac caagctcttt gattctttca
     3901 tatacaacat ttaccatccc attctctgtc tggcttctga ctggctacct cgatgattta
     3961 cctcgatcgc ttgaagaggc ggcaatggtg gatggagcga atcgttttca agcatttata
     4021 aagattattt tacctatgct tttgccttgt gttgtcgcta caggcgcata tattttcatg
     4081 acttcgtgga atgaatatac cttcgctgtc atgtttacca atgaacgaaa tcggacgatt
     4141 cccgttgcac ttaaaaattt cattggtcag ttaggtgttg aatggggaat gctgacagct
     4201 ggaggcgtaa tcacaataat tcctgtttgc ataatgtttt tctttgctca aaaaagacta
     4261 gtcgaaggct taactgcagg cgcagtgaaa gggtaataaa ggaatactca aaatggatga
     4321 acttctgctg gaacaaaagg cgcaagctat aaggcgcgac attatcgaaa tgctctatcg
     4381 atgtcaatct ggccacccgg gcggatcgct ctcatgtgtg gaaatattaa tggcgctcta
     4441 ttatgaggtt gcgcatgtcg atcctacaaa tccatcgtgg gagatgcgag ataggatcgt
     4501 gctgtctaaa ggccatggtt gccctgcgct ctacgcgatt ctcgctgata aaggtttttt
     4561 ccctcgaaat gatctttgga acttgaggca gatcggtagc catcttcaag gacatcctga
     4621 tatgcgcaag actcctggca tcgatgtatc gactggctca ctaggtcaag gcgcctctgt
     4681 agctg
//
LOCUS       DAOMZL010000112         4604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_17667_length_4604_cov_15.570675, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000112 DAOMZL010000000
VERSION     DAOMZL010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17667_length_4604_cov_15.570675"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PLQ30_07565"
     CDS             <1..809
                     /locus_tag="PLQ30_07565"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HPW02343.1"
                     /translation="IYPYQVWLDGLYMFGPFQAHYGILFGQTDMVDDVCQQFLFVRDK
                     LRHRQTGLYFHAIDDSKASRWADQETGCSPHVWARAVGWLAMALVDTLDWIPLAHSKR
                     PYMEEMLMDLLDALVREQRSSGLWLQVMDAPEAKSNYEETSASAMFAYTFYKSLGRGL
                     VTNANRAKIWKKTADSAIAGIITKKVRWECASEPSIQLLQIEPMAELSTFKSTMPRLH
                     LDGICSVAGLGGSPYRDGSIAYYVGEPIVLDDFKGLGPFMLALTEALHAN"
     gene            complement(811..1809)
                     /locus_tag="PLQ30_07570"
     CDS             complement(811..1809)
                     /locus_tag="PLQ30_07570"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW02344.1"
                     /translation="MNDSAILISIGGVLASAAPIIFAVIGETISERAGVLNLSMNGLI
                     LICAMGSFAVALATDSSILGFLTGAAIGALVSLIVAFASLTLLQSQVAVGFVLAMLCK
                     DLSYFLGSPIMGKVAPRISQVPIPLLERIPILGPIFFRQDMMTYLSFVLIVLAWFFIF
                     KTKPGLILRGIGERPAAAYIRGANVTRLQYLYAVAGGAIAGLAGPIYSLSIKAGWKGT
                     ISGLDGIGWIVLSITIFGGWKPFRAAFGAYLFSFLQWLGLVLQPSLPSIPSQVLQVAP
                     FPLMILTLLFVNIGNAEWVERSLARLPENVRRDIAKVLRALNATPPAALGEPFRKE"
     gene            complement(1806..2867)
                     /locus_tag="PLQ30_07575"
     CDS             complement(1806..2867)
                     /locus_tag="PLQ30_07575"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW02345.1"
                     /translation="MPSLREKKASGVLFQIAAVLFALLITSIVLLLANVEPFSAYKAI
                     ILGAVGSWDLMSNVLVSWVPLLLATSGLLLTFTVGLWNIGIEGQITLGAIFTTWAMRL
                     LQNSEWNPSLILVIAIFAGMLGGMLWAMLAGALKTFGGVNEIFGGLGLNFVATALNIW
                     LIFGPWKRPGVASMSGTLPFDRSLWMPIATGSSRLTPTTIVMAIVGILIVYVLLENTY
                     FGLKLKAVGKNPRSAYLLGVPTWQHMMASFAICGVFAGLAGAVQVTAVYHRLIPSISS
                     GYGYLGLMVAMLVGYKASLAAPVALFFAALNVGSIQLPIILRLDSSLAGVLQGTLVLC
                     VLLGQGFRAKFLKKKRTMQ"
     gene            complement(2851..4359)
                     /locus_tag="PLQ30_07580"
     CDS             complement(2851..4359)
                     /locus_tag="PLQ30_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW02346.1"
                     /translation="MQVEFRGITKCFGPLRANDDISFIIPSSTIQGLLGENGAGKSTL
                     MKILSGYYSSDAGEIYLNGKKVDISCPGDAIDLGIGMLHQDPLDFPPLKVIDNFILGK
                     KGGILPNRGSAIREFEELADRFDFSIDPEAYVDSLTVGERQQLEILRLLWLGARVLIL
                     DEPTTGISLPQKEKLFATLKKLAEQGMTILFVSHKLEEVEQLCDRAIVLRQGKLIGAM
                     DPPFNTKALVEMMFGKEIELEYRAKLSIGQKVLHAKSICLEGTRINIKGVDLEVRAGE
                     VIGLAGMEGSGQTMFLSACAGLTQPVGGSLCLGEEDKDMTGRSYLEFLKHGVAYLPAA
                     RLEQGLICGLTLMEHFVLSEKMPGFFIDRKRSEELTKERIALYNIKGAPDSPIESLSG
                     GNQQRALLALLRDHLELILVEHPTRGLDYESTIYIWGKLKERCANGTAIIFISSDLEE
                     ILTYSDRVLVFFAGKVTPPLCAEKLNVERLGRLIGGKDWENLEKEAVYAVFA"
BASE COUNT         1290 a         1143 c         1066 g         1105 t
ORIGIN      
        1 agatatatcc gtatcaagta tggctcgatg gtctttatat gtttggacct tttcaagcgc
       61 actatgggat cctctttgga caaacggata tggtagatga tgtatgccag cagttcttgt
      121 ttgttcggga taaattgagg catagacaaa ctggactata tttccatgcc atagatgata
      181 gtaaggcatc tagatgggca gaccaggaaa ctggctgttc gcctcatgta tgggctagag
      241 ccgttggctg gcttgcaatg gcgctcgtgg atacgctcga ttggattcct cttgcgcatt
      301 ctaagcgacc ttacatggaa gagatgctta tggatcttct cgatgctctt gtgcgagaac
      361 agcgttcttc tggcctatgg ctgcaggtta tggatgctcc agaggctaag agcaactatg
      421 aggagacttc ggcttcggcc atgttcgctt atacattcta caagagcctt ggaagaggtt
      481 tagtaacgaa tgcaaatcga gccaaaatat ggaagaaaac cgcagactca gcgatagccg
      541 ggataataac aaaaaaagtc agatgggaat gcgcttctga accttcgata cagcttctac
      601 aaatagaacc gatggcagaa ctatcgacat ttaagtctac aatgccgcgc ctgcatctcg
      661 atggaatatg cagcgttgct ggtcttggtg gatctccata tcgcgatggc tcgattgcct
      721 actatgtcgg agaacctatt gtattggacg attttaaagg ccttggtcct tttatgcttg
      781 cattaaccga agctctccac gccaattaaa tcattccttc ctgaatggct cgccaagagc
      841 tgcaggtggg gtggcattca gggctctcaa gaccttggct atatctctac ggacattctc
      901 gggcaaacgc gcaagcgatc gctcgaccca ctccgcgttg cctatattca caaatagcag
      961 tgtcaggatc ataagaggga agggtgccac ctgtagcact tgggatggga tagatggcag
     1021 actgggctgc aacacaagac ctaaccattg aaggaaagaa aaaagatatg ctccaaaagc
     1081 tgccctgaat ggcttccagc cgccaaagat cgtaatggag agtacaatcc agccgatacc
     1141 atcgagcccc gaaatagtgc ccttccaccc tgcctttatg gaaagcgaat aaatagggcc
     1201 tgccagtccg gcaattgctc ctcctgccac cgcatacaga tattgcaagc gggtcacgtt
     1261 tgcgccgcga atataagctg cggcgggtcg ttctcctatg cctcgtagaa tgagaccggg
     1321 cttggtctta aagataaaga accacgcaag cacaatcagc acaaaagaaa gatatgtcat
     1381 catatcctgg cgaaagaata tggggccaag aattggaatt cgttcgagca aaggaatagg
     1441 tacctgtgag atacgtggtg ctacctttcc catgattggg ctgccaagaa aataagacaa
     1501 atccttgcat agcattgcca acacaaagcc aacagccacc tgagactgca aaagagtcag
     1561 gcttgcaaaa gccactatga gcgagacaag agctcctatc gctgctcctg tcaaaaaacc
     1621 caagatcgag ctatcagtcg ccagagccac cgcaaaacta cccattgcgc agattagaat
     1681 caacccattc atggaaagat tcagcactcc tgcccgttcg gagattgtct caccaatgac
     1741 agcaaagatg ataggcgcag ccgaagcgag caccccaccg atacttatta gaatcgcaga
     1801 atcgttcatt gcattgtcct ttttttcttt agaaacttag cgcggaaacc ctgtccaaga
     1861 aggacgcaaa gaacaagcgt tccctgaagc actccggcca atgaagaatc caacctgagg
     1921 atgataggca actgaataga gccgacattc agagcagcga agaacaaggc aaccggagcc
     1981 gcgagacttg ccttataccc tactaacatg gctaccataa gccccaaata accatagccc
     2041 gatgaaatcg aagggataag gcggtggtaa acggcggtaa cctgcactgc tccggcaagg
     2101 cctgcaaaaa caccgcagat ggcgaaagac gccatcatgt gctgccaggt cggaacaccc
     2161 agcaggtatg cgctccttgg attcttgcct actgccttta gctttagacc aaaataggtg
     2221 ttctcaagca acacatagac aatcaagata ccgactatag ccatgacaat agttgtaggc
     2281 gttagcctag atgaaccagt agcgattggc atccataggg agcggtcgaa gggcaaagta
     2341 cctgacatcg aagcaacacc aggacgtttc catggtccaa agattagcca gatattgaga
     2401 gctgtcgcta caaagttgag tccaaggcct ccgaaaatct catttactcc tccgaaagtc
     2461 ttgagcgcac ccgcgagcat agcccatagc attcctccta acatcccagc aaagatcgca
     2521 atgaccagaa tgagtgaagg attccattcg gagttctgca aaagccgcat agcccaagtc
     2581 gtaaatatcg cgccaagcgt gatctggcct tctattccaa tgttccacaa gcccaccgta
     2641 aaggtcaaaa gtagccctga ggttgccagg agcaaaggca cccaggaaac gagaacattg
     2701 ctcatcaaat cccaggaacc tactgctcct aagattatgg ctttataggc agaaaagggt
     2761 tcgacattag ccagaagaag cacgatagat gtaatcaaca gtgcgaataa aacagctgct
     2821 atctgaaata gaacgccact ggcctttttt tcacgcaaag acggcataaa cagcttcctt
     2881 ttccaggttt tcccaatcct taccacctat gagtcggccc aaacgctcca cattgagctt
     2941 ttctgcacaa agtggcggcg taaccttgcc tgcaaagaac acgaggactc tatcgctata
     3001 tgttaggatt tcctcgagat ccgaagagat gaaaattatt gcggttccgt tagcacatct
     3061 ctctttgagc ttgccccaaa tatagattgt cgattcgtag tcaagacctc gtgttggatg
     3121 ctcgaccaat atgagctcga gatgatctcg cagaagcgca agaagagctc tctgctgatt
     3181 acccccggaa agcgattcaa ttgggctgtc gggtgctccc tttatgttgt agagagcgat
     3241 gcgctctttt gtaagctctt ccgatctttt gcgatcgatg aagaaaccgg gcattttttc
     3301 cgaaagcaca aaatgctcca tcagagtaag cccacagatt agtccttgct ccaaccttgc
     3361 cgcgggtagg taagcaacgc catgcttgag aaactccaga taagagcggc ctgtcatatc
     3421 tttgtcttct tcgccaaggc agagtgagcc gccgacgggt tgagtaagcc cggcacaggc
     3481 actaagaaac atagtctggc cagaaccttc catacctgca agaccgataa cctcgccagc
     3541 cctaacttcc agatcgactc cctttatatt tattctagtt ccttccaggc agatactttt
     3601 tgcgtgcagc actttttgtc ctattgatag cttagccctg tattcgagtt ctatctcttt
     3661 accgaacatc atttcgacaa gcgccttggt attgaaaggc ggatccatag ctccaatcag
     3721 ctttccctgt cgaaggacga tagccctgtc gcaaagctgc tccacttctt cgagcttatg
     3781 agatacaaaa agaatggtca tgccttgttc tgctagtttt ttcagagtgg caaagagttt
     3841 ttctttttgt ggcaggctta tgccggttgt gggctcatcg agaataagca cccgagcgcc
     3901 taaccacaaa agacgcaata tctctaactg ctggcgctcg cccacggtaa gagaatccac
     3961 ataggcttct ggatctatcg aaaaatcgaa gcggtctgca agctcttcga actctctaat
     4021 tgcagaacct cggttgggca gaatccctcc ttttttaccg agaatgaagt tgtcgattac
     4081 cttgagaggc gggaaatcca atggatcttg atggagcata ccgattccca aatctatggc
     4141 atcaccggga caagagatat cgactttttt gccatttaag tagatttcgc cggcatcaga
     4201 gctgtaatag cccgagagta tcttcatgag agtgctttta cctgccccgt tctctcccag
     4261 aaggccttga atggtagagg agggaattat gaatgaaata tcgtcgtttg ctcgcaatgg
     4321 gccaaagcac tttgttattc cacgaaactc gacctgcatt gaatccttct tctagtctat
     4381 ttgatctcgc tcatttaaga ttgaagcaat gagcgtcaat tcaagtctga agcatgtcaa
     4441 aaatagagcg cggaggcaag ccgaccaagg accgtcagct tatgcctccg acatactaga
     4501 gaaaaagctc tctactatat gaaataaaga atacaattac ttcgatactg attgtccttc
     4561 catgccctgc aagagctgtg gcaggtacca gatctgctga tctg
//
LOCUS       DAOMZL010000113         4544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_17943_length_4544_cov_13.884830, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000113 DAOMZL010000000
VERSION     DAOMZL010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17943_length_4544_cov_13.884830"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1039
                     /locus_tag="PLQ30_07585"
     CDS             152..1039
                     /locus_tag="PLQ30_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPW02347.1"
                     /translation="MQYDIVMMGHISKDIIIDETGNESKLYGGALLYSSIAAARAGAH
                     VLAITKAAPEDFCALDIAKEQNGIEIIAIQSPKTTSIRNVFLSADHERRETSLLSRAE
                     PFLAADIPSNAETKIIDLAGLFVGELPDNLIEELAPRGAIAVDAQGLLREAQPDGRML
                     FRDWRNKHHYMPFVSYFKADAAEAEILTGLADREEAAREIAAWGKHPDGHEPEVMITY
                     NTEVIVLVDGRIYRAPYKPSNLSGRTGRGDTTFAAYLAWRLGHDPQESVRFAAALCSI
                     KMERPGPFSGTLEDVFARM"
     gene            complement(1123..3546)
                     /locus_tag="PLQ30_07590"
     CDS             complement(1123..3546)
                     /locus_tag="PLQ30_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPW02348.1"
                     /translation="MAETQKTESRTIAQNAKGNRLSQSAKVLRDKVRRIIARAQSEHR
                     EALTELEGMSILAAMGVQTPRYWLVQSAKEFLEKIGAKGAVDAPLQGPFSGSKAVIKI
                     MSSQVLHKTEVQGIEIVDNTAAAIADSLFRMEARFSNVPRDGFSVNEFIAFEPKLGHE
                     MIFGYRFTPDFGPIVSFGPGGIYTEYLASKFKPGSANLFLSPRVTDRAVLESLLENNV
                     IYGLLCAGLRNTTPELDEKSLIEAIQRFIDAADALSAAGISEFEVNPMVLSKTGELVA
                     LDCLVTLREFAAPAPVNASVQGSAQNLIQGLVRDSEGLPINLAQQTRPVDQIGHILEP
                     SSVAIIGVSEKTINNGRIILRNLLENGFDISRIYVIKPGAQWIDGCRCVADVQSLPEK
                     VDLFVVVIPALSIPSTLADIARYDKAWSVLLIPGGLEEKEGSESIVAEMNRALAEARA
                     KGKGPLINGGNCLGIRSVPGKYNTLFIPEYKLPMPKGKVEPLAVISQSGAFAITRISK
                     HPNINAKYAITIGNQMDLTVGDYLDYLAQDNEIHVFAVYSEGFKPLDGEKALKATKKI
                     SESGRSVLLYRGGRTRAGAGAAASHTASIAGEYPVTWQLFSQAGAIVCESLDEFDDAI
                     TLFTLLDGKRAKAKRLAAVSNGGYECVAIADNLGEMMLSTFNEKTKAELEVIFKDARI
                     TGIVDIHNPLDLTPMADDAAYERALRAVLLDPESDLAIVGIIPLTAMLNTLASNPLSH
                     SEDILRPNSLPERLARLASEIDKPFIAVVDSGPLFDPLCAELEKRGIPTFRAADRALR
                     MLERWRKSH"
     gene            complement(3539..4141)
                     /locus_tag="PLQ30_07595"
     CDS             complement(3539..4141)
                     /locus_tag="PLQ30_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPW02349.1"
                     /translation="MAKESEAIMTCDIILCSVGGQGGLSASIILARATMASGFQVKQS
                     EIHGMSQRGGEVLAHLRIADKEIYSPTISKGKADIMFAFEPLEALRHLSWISPSNGTI
                     VSATTPVLNISNYPDLEIVLAEIRKIPRARLIDAEAIARQAGNVRTANLVLIGAAMDL
                     LPISPKAIEKEIIELFSRKGESVIQANLKAFEAGRALYHG"
     gene            complement(4114..>4544)
                     /locus_tag="PLQ30_07600"
     CDS             complement(4114..>4544)
                     /locus_tag="PLQ30_07600"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPW02350.1"
                     /translation="QPPWNAIESCVDMGASVGMARGASCAGHKRSIAVIGDSTFYHSG
                     MTNLLDAVRHNSPMTLIILDNGTTGMTGAQPTISPSQQLPKLLEGLGVEKEHIRVLEA
                     HRRALDVNVEIIREELCYDGVSVIIMFRECIEWLKKARQS"
BASE COUNT         1161 a         1108 c         1108 g         1167 t
ORIGIN      
        1 aagtttaaca aaatgcaact gaaatcgttt gcaggatgct aaaaacgttt tcataaattt
       61 gctagacaaa tgtaaaggag atcccaaaga ggagattagt caaagaattt caaagaggag
      121 attcttaaat aatttctaag aggagatgcg tatgcaatat gacatagtga tgatgggtca
      181 tatttcgaag gatataataa tcgacgagac agggaatgag agtaagctct atggcggagc
      241 gctcttgtat tcatctattg cagcggcacg agctggagcc catgtactcg ccattaccaa
      301 agctgctcca gaagattttt gcgcactcga tatagcgaag gagcagaatg gcatagaaat
      361 catagctatc cagagtccca aaactacatc gatacgcaat gtatttctat cagcggacca
      421 tgaacgaaga gagacaagcc ttttatctcg ggcagaacca tttttggcag cggacatacc
      481 ttcgaatgca gagacgaaaa taatcgatct ggcaggtctc tttgtaggcg aattgcctga
      541 taatctcata gaagaacttg cgcccagagg ggctattgct gtcgatgcgc aaggacttct
      601 tcgtgaggct cagcccgatg gcaggatgct tttcagagat tggaggaata agcatcatta
      661 catgcctttt gtgagctact ttaaagccga tgccgccgaa gcggagatac tgacaggtct
      721 tgccgacaga gaagaagccg ctcgggaaat agcagcttgg ggcaaacacc cagatggcca
      781 cgaacccgag gttatgatca catataatac cgaagtgatc gtgttagtgg atggaagaat
      841 ctatcgcgcg ccctataagc cttcgaatct ctccggaaga actggacgag gcgacactac
      901 ctttgctgcc tatcttgcct ggcgccttgg ccacgatcca caggaatcgg tccgatttgc
      961 agcagcactc tgttccataa aaatggaaag gccaggacct ttctcaggaa cactcgaaga
     1021 tgtgtttgcg cgtatgtagc gcatctgagt acgcatctaa ttcctaatgc cttaaagcaa
     1081 ctctaatagc ttatattctt tcattttgat gctctatgtt tttcaatgcg attttcgcca
     1141 gcgctccagc attcgcaaag ctcgatccgc agcgcgaaaa gtcggaattc cgcgcttttc
     1201 gagctcagcg cagagaggat cgaagagcgg accagagtct acaacggcaa tgaaaggctt
     1261 atcgatttcg ctcgccaacc ttgcaagtcg ctcgggaagc gaattgggcc taaggatatc
     1321 ttcgctgtga gataagggat tgcttgcaag cgtattcaac atggcagtca acgggattat
     1381 cccaacaatg gcaagatcgc tttctgggtc cagaagtacc gccctaagtg cccgttcgta
     1441 ggcagcgtcg tccgccattg gggtgagatc gagtgggttg tggatatcga ctatccccgt
     1501 tatgcgcgca tccttgaaaa tcacttcgag ctcagccttt gtcttttcat taaaagtaga
     1561 caacatcatc tcgcccagat tgtcggcaat tgcaacgcac tcatagccgc cattggagac
     1621 agccgcaagc cttttggctt ttgctctctt gccgtcgagt agtgtaaata atgtaattgc
     1681 atcgtcgaat tcatccagac tttcgcaaac gatcgcgcca gcttgagaaa agagctgcca
     1741 agtaacagga tattcgcccg caatactggc tgtgtggctc gccgccgccc cagctcctgc
     1801 tcgcgttctg ccccctctat atagaaggac actgcgacca gactcagata tctttttggt
     1861 ggctttaagg gctttttctc cgtccagcgg cttgaacccc tcagaataga ccgcgaacac
     1921 atggatctcg ttatcttgag ccaggtaatc cagataatct ccgactgtta ggtccatctg
     1981 attgccaatg gttattgcgt attttgcatt gatgttggga tgcttggaga tgcgtgttat
     2041 cgcgaaggca cctgactgag agatcacagc aagcggttct actttgcctt taggcatggg
     2101 cagcttgtat tcagggatga aaagggtatt gtatttgcca ggaaccgagc gtatgccaag
     2161 acaattgcca ccatttatca atggtccttt tcctttggcg cgcgcttcgg ccaaagcgcg
     2221 attcatctcg gcaactatgg attctgagcc ttctttttcc tctaggcctc cggggattaa
     2281 aagaaccgac cacgctttgt cgtatcgcgc aatgtcagca agcgtgctgg ggatactaag
     2341 cgctggaatt accacgacaa agagatcgac tttctcaggc agagattgaa catctgcaac
     2401 gcatcgacag ccatctatcc attgagcacc tggctttatc acatagattc tagatatatc
     2461 gaagccattt tcaaggagat tgcggagaat tatgcgaccg ttgttgattg tcttttcaga
     2521 tacgccgatt atagccacac tcgaaggctc gagaatgtgc cctatttgat cgacaggccg
     2581 agtctgctga gctaaattta ttggcaagcc ttcggaatct cggacaaggc cctggatcag
     2641 attctgagca gagccttgca cagaggcatt cacgggtgcg ggcgctgcaa actctcttag
     2701 agtgacaagg caatccagtg cgacgagctc gcctgttttt gaaagcacca ttgggttgac
     2761 ttcgaattcg cttatacctg cagctgaaag ggcatctgca gcgtctataa agcgctggat
     2821 ggcttcgatt agactctttt cgtctagctc tggcgttgta tttcttaagc cagcgcacaa
     2881 aagtccatag attacattgt tttctagaag cgattcgagc acagctctat ctgtaactct
     2941 tggagagaga aaaaggttcg ccgagccagg cttgaatttg gatgcgaggt actcggtata
     3001 gataccgcca gggccgaaac tcacgatagg cccgaaatca ggcgtaaaac gataaccaaa
     3061 aatcatctcg tggccaagct tcggctcgaa agctatgaac tcattgacac taaaaccatc
     3121 gcgagggaca ttactaaagc gcgcttccat cctgaagagc gaatcggcaa ttgctgctgc
     3181 tgtgttgtcg acaatttcga tgccttgtac ttcggtctta tgaagaacct gagaactcat
     3241 aatcttgatt acagccttgg agccagaaaa agggccttgc agaggagcat ccactgctcc
     3301 cttagctccg attttctcta gaaactcctt tgccgactgc acgagccaat accgtggcgt
     3361 ttgtacgccc attgcagcca gaatagacat tccttcgagc tcggtaagcg cttctctgtg
     3421 ctcactctgg gctcgtgcta ttatgcgcct taccttatct cttagaactt ttgccgactg
     3481 actcaggcga ttgcctttcg cgttttgagc aatcgttcga ctttcggtct tttgtgtttc
     3541 agccatggta caatgctctc cctgcttcaa aagccttgag attggcttgt atgaccgact
     3601 cgccctttct agagaagagc tcgattatct ccttttcgat agctttcgga gaaataggaa
     3661 ggagatccat tgcggcgcca attagaacaa ggtttgcagt tctcacattt cctgcttgcc
     3721 gtgctattgc ttctgcatcg ataagccgag cgcgagggat tttgcgaatt tctgccaaca
     3781 caatttctag atcggggtaa ttgctaatgt tgaggacagg tgtagtggct gagacaatcg
     3841 taccattaga gggcgagatc cacgaaagat gacgaagcgc ttccagaggc tcgaaagcaa
     3901 acataatgtc cgctttgccc ttggaaattg tgggactata gatctcctta tcggcgatgc
     3961 gcaagtgagc caagacctca cctccgcgct gggacattcc atgtatctcg gattgcttaa
     4021 cctgaaaacc agaggccatt gtggcacggg ccaagattat cgatgcagaa agtccaccct
     4081 ggcccccaac gctgcagaga attatatcgc aggtcatgat tgcctcgctt tctttagcca
     4141 ttcgatgcat tcccggaaca ttatgatgac cgagacacca tcgtagcaaa gctcttcgcg
     4201 aatgatctcg acattgacat ctaatgcacg gcgatgcgct tctaggacac ggatatgctc
     4261 tttttcgaca ccaagccctt cgagcagctt tggaagctgt tggcttggac tgattgtagg
     4321 ctgagctccg gtcatgccgg tagtcccatt gtccaagata atcagggtca taggactatt
     4381 gtgtcgcaca gcatcgagca agtttgtcat gcccgaatga taaaaggttg aatcacctat
     4441 gactgcgatg gagcgcttat gtccagcaca acttgcgcca cgcgccatac ccaccgaagc
     4501 gcccatgtcc acacaggact ctatggcatt ccaaggtggc tgtg
//
LOCUS       DAOMZL010000114         4536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_17994_length_4536_cov_14.370230, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000114 DAOMZL010000000
VERSION     DAOMZL010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17994_length_4536_cov_14.370230"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PLQ30_07605"
     CDS             complement(<1..887)
                     /locus_tag="PLQ30_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPW02351.1"
                     /translation="MQYDITMMGHISKDIIIDESGNESKLYGGALLYSSIAAARAGAH
                     VLAITKAAPEDFCALDIAKEHSGIQIIAIQSPKTTSIRNVFLSADHERRETCLLSRAE
                     SFSAADIPLNAETKIIDLAGLFVGELPDNLIEELAPRGAIAVDAQGLLREAQPNGRML
                     FRDWNNKHRYMPFVSYFKADAAEAEILTGLTDREEAAKKIAAWGRDSEGKEPEVMITY
                     NTEVIVFVDGKIYRAPYTPSNLSGRTGRGDTTFAAYLAWRLSHDPHESVRFAAALCSI
                     KMEKPGPFSGTLEDVFARM"
     gene            1076..2107
                     /locus_tag="PLQ30_07610"
     CDS             1076..2107
                     /locus_tag="PLQ30_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW02352.1"
                     /translation="MRKIFLSVLMLFCLLPLMSTSAQATPDYKSLTVYTALPESELPT
                     YFSQFEKDTGIKIQYVRLSAGELLARVRAEKNNPQATVWFGGSYDNFVPAISEGLLEA
                     YQSPELVNIPKVYWDVKGYANPFYVGAIGFACNTDWFKKKGLAYPTSWDDLLKPEFKG
                     QISMAHPSTSGTSYTILATIVQMRGEEATWKYFSALNQNIRQYTKSGTTPPMDVGLGE
                     AAIGITFSHDGLKPAFEGYPVALSFPKDGTGYEIGCMALIKNGPSKETSNGKRFIDWM
                     LSKRGQDLFETSKSFRIPVNSQATPPKGSISVDSLKVIDYDPIWAGQNRNRLIEQFNK
                     VVAAASNLK"
     gene            2222..3913
                     /locus_tag="PLQ30_07615"
     CDS             2222..3913
                     /locus_tag="PLQ30_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPW02353.1"
                     /translation="MRENTRLSVAHLVRDPVLLFLVILVFVGLFLFIIFPLCKIFAYS
                     VTDNDGNLSFQAAWDMLKSRSYLQPLKNSMMLGITTGILATIIGYVFAYALTRVDMPL
                     KGFFKTIATIPIISPPFILSLSMIFLFGRNGLITRRLLGIEDANVYGMHSLVVVQTIS
                     FFPIAYLTLTGTLSKLNPAVEDAALNLGASKARIFRTVTLPLSVPGILSSFLLVFIQS
                     MEDFSNPAVISGSFSTLAVEAYRTITGMYDMRGGSLMALLLLIPTLIAFLLQKYWLSG
                     KSFVTVTGKPTVSQQQSKDPKMIWPLFAFCMLVALVILLFYGTVLVGAFVKVWGINFS
                     FTWAHFKYVMTLGWIPLRNSVVLAMASTPIAGLLGMIIAFLVVRKEFPGRRAMEFISM
                     LTFAVPGTVVGIGYILAFNDKPFMWTGSAFLLIMAFAFRNMPVGIEAGTSTLLQIDRS
                     IEEASTILGATGAVTFRRISLPMLKQAFFSGLVYSFVRAMTAVSTVIFLISPRWNLAT
                     ISIFSLFESSKYSDAAAYIIVMILFIVVAIGVLNLLVGFLGRGGPSASGGSSERA"
     gene            3900..>4536
                     /locus_tag="PLQ30_07620"
     CDS             3900..>4536
                     /locus_tag="PLQ30_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW02354.1"
                     /translation="MSERDFSARSSNVSLIDLTKRFKSLDGSGEVLAVDDVNLEVKTG
                     ELVTILGPSGCGKTTTLRMIAGFEYPTSGSILIGDKDVAKIPPNKRGLSMVFQSYALF
                     PHLTIYENVAYGLRVQKLPAREIRERTERALDLMQLTTMAKRYPSQVSGGQQQRIALA
                     RAIVIEPSVLLFDEPLSNLDAKLREYMRDELRKLQKRLGITSLYVTHDQSEA"
BASE COUNT         1108 a         1085 c         1059 g         1284 t
ORIGIN      
        1 tacatacgcg caaacacatc ttcgagcgtt ccggagaaag gcccgggctt ttccattttt
       61 attgagcaaa gagccgctgc aaatcggacc gattcatgtg gatcgtggct gaggcgccag
      121 gcaagatagg cagcaaaggt ggtgtcgccc cgccccgttc ttcccgagag attcgaaggc
      181 gtatatgggg cacgatagat ctttccatcc acaaaaacga tgacttcggt attatatgtg
      241 atcatgactt ctggttcctt gccctctgag tctctgcccc aggccgctat tttctttgcg
      301 gcttcttctc tgtcggtaag acctgtgagg atctccgctt ccgcggcatc tgctttgaag
      361 tagctcacaa aaggcatata gcgatgtttg ttattccaat cgcgaaacag catcctgcca
      421 ttgggctgag cctcgcgaag aagaccctgg gcatcgaccg caatagcccc acggggtgca
      481 agctcttcta ttagattatc aggcaattcg cctacaaata gtcctgccag atcgattatc
      541 ttcgtctctg cgttcaaagg gatatcagcc gccgagaatg attctgcccg agataagaga
      601 caggtctctc ttcgttcatg atccgcagac agaaatacat tgcgaatcga tgtcgttttg
      661 gggctctgga tagctatgat ttgtatgccg ctgtgctcct tggctatatc taatgcgcaa
      721 aaatcctccg gagcggcctt agttatggcg agtacatggg ctccggctcg cgcagcagcg
      781 attgatgaat acagaagtgc tccaccataa agcttgcttt cattcccaga ctcgtcgatt
      841 attatatctt ttgaaatatg acccatcata gttatatcgt attgcattat ctttctcctt
      901 ccaggattct tctttataga atctcctctt acggatctcc tttgcctctg tacagcaaat
      961 atataaaata gttcttacca tcctgccatc ctgcaaacga tttcagttgc attttgctaa
     1021 atttgatcta taatattgtt ctataataga cgaggaactt gaaaggaggc aatctatgcg
     1081 caagatcttt ctatcggtac taatgctgtt ctgcctattg cccttgatgt caacaagcgc
     1141 gcaggcaaca ccagattaca agtcactaac cgtttatacg gctctgccgg aatccgagct
     1201 cccgacatat ttcagccagt tcgaaaaaga tactggaatc aagatccaat atgtcagact
     1261 ctctgctggc gagctgcttg ctcgtgtaag agcggaaaag aacaatcccc aggctaccgt
     1321 ctggtttggc ggttcctacg acaactttgt acccgcaatc tccgagggtc tcctcgaagc
     1381 ctatcagtct cctgaacttg taaacattcc gaaggtgtac tgggacgtaa agggctatgc
     1441 aaatcccttc tatgtgggag caattggctt tgcatgcaac accgattggt tcaagaagaa
     1501 aggcttggcc taccctacct cttgggatga tctcttgaag cccgaattca agggtcaaat
     1561 ctcgatggct cacccaagca cttcaggaac ttcctataca atccttgcaa ctattgtcca
     1621 gatgcgtggt gaagaagcca cctggaaata cttttccgct ctcaatcaga atattcgtca
     1681 gtataccaaa tcggggacga ctcctcctat ggatgtaggc ctcggagaag cggccattgg
     1741 aatcacattc tctcatgatg gcctcaaacc ggcattcgaa ggctaccctg ttgccctatc
     1801 cttccctaaa gatggcacag gttacgaaat tggctgcatg gcgctgataa agaatggccc
     1861 atcaaaagaa acatcaaatg gcaaacgatt tatcgactgg atgttgtcaa agcgcgggca
     1921 agacctcttc gagacttcta aatcctttag aataccggta aattcacagg caactccacc
     1981 caagggctca atttcggtcg acagtctcaa ggtcatcgac tatgatccca tatgggctgg
     2041 tcagaacaga aatcgcctaa tcgagcaatt caataaagta gtcgcggcgg caagcaacct
     2101 gaagtagaat tgatcaggta gattgagcat ttatggaggt gcgcgaaatt ttctttgcgc
     2161 acctcttgtg caaaagcgat atgactgcac aatcttcatt ccaaaggaac ctgagatttt
     2221 aatgagagaa aacactcgcc tttcggtagc gcatcttgtg cgtgatccgg tacttttatt
     2281 tctagtaatc ctggtattcg tagggctttt tctttttata atatttcccc tatgcaagat
     2341 atttgcatat agtgtaacgg ataatgatgg caatttgagt tttcaagccg cttgggatat
     2401 gttaaaatct cgctcatatc tccaaccgct caagaattct atgatgttgg gtattacgac
     2461 aggtattctc gcaactatca ttggatatgt ctttgcatat gccctgactc gtgtagatat
     2521 gccactgaag ggatttttca agacaatagc gactatcccc ataatctctc cgccttttat
     2581 tctttctcta tcgatgatct ttctttttgg gcgaaatggg ctgataactc gcaggctttt
     2641 aggcatcgaa gacgcaaatg tctatggaat gcatagcctt gttgttgtcc agacaatttc
     2701 tttttttccg atagcctacc ttacgctgac cggcactctt tcgaaattaa atccagcggt
     2761 ggaagatgca gcactgaatc ttggtgcctc taaggctcga attttccgaa ctgtaacttt
     2821 gccgctttcg gtgccgggta tcctcagctc ttttttgctt gtgttcattc aatccatgga
     2881 ggatttcagc aatccagcgg tcatttcggg aagtttctcc actcttgctg tagaggcgta
     2941 cagaaccatt acaggcatgt acgatatgag aggcggttct ttgatggcgc tcttgctact
     3001 cataccgacc ctgatcgcct ttctgttgca gaaatattgg ctatcgggca agtcttttgt
     3061 aacagtcacg ggaaaaccca ctgtatcgca gcagcagagc aaggatccta agatgatatg
     3121 gcccctcttt gcattctgca tgctggttgc gctggttatt ctcttgttct atggaacggt
     3181 tctcgtgggc gcttttgtca aggtctgggg cataaacttt agctttacct gggcgcactt
     3241 caaatacgta atgactctcg gttggattcc gctgagaaat tccgttgtgc tcgccatggc
     3301 gtctacgccg attgctgggc ttcttggaat gatcattgct ttcctggttg tacgcaagga
     3361 attccccggc agacgagcaa tggagtttat ctccatgctg acttttgcgg tccctggaac
     3421 tgttgtggga attgggtata ttctcgcatt caatgacaaa ccatttatgt ggacaggatc
     3481 ggcttttttg ctaataatgg catttgcatt cagaaatatg cctgtgggaa ttgaagcagg
     3541 aacttcgacg ctcttgcaga tcgatagatc gatcgaggag gcttcgacaa tactcggagc
     3601 tacaggggct gttacttttc ggcgcataag cttaccgatg ctcaagcagg catttttttc
     3661 cggccttgta tactcattcg ttcgcgccat gacagcggtg agcacagtaa tctttcttat
     3721 ctcgccgcgc tggaatttgg ctacaatcag cattttttct cttttcgaat cgagcaagta
     3781 ttcggatgcc gcggcctata ttatagtcat gatacttttt atcgttgttg ctatcggcgt
     3841 gctcaacctt ttggtgggct tcctgggtcg aggcggtcct tcagcaagtg gaggcagcag
     3901 tgagcgagcg tgatttcagt gcaagaagtt ccaatgtgtc tcttattgat ctaacaaaga
     3961 ggtttaaaag tctcgatggc tctggcgagg ttttggctgt cgatgatgtc aatctggaag
     4021 tcaaaaccgg cgaactggtg acgattcttg gtccatcggg ctgtggtaag acaacgacgc
     4081 tcagaatgat agcaggcttc gagtacccta cttctggctc gattcttatt ggggataagg
     4141 atgtagccaa gattccgccc aacaagcgcg ggctatccat ggtctttcag agctatgccc
     4201 tgtttccgca tctaacgatc tatgagaatg tggcctatgg actacgggta caaaagctgc
     4261 cagccaggga gattcgcgag cgtactgagc gtgcgctaga tctcatgcag ctaacaacta
     4321 tggccaagcg ctacccgagc caagtgtccg gcggtcaaca acagagaatt gccctggccc
     4381 gcgctatagt gatagaaccc agtgtgcttc ttttcgatga accgctctcg aacctcgatg
     4441 ccaaacttcg agaatatatg cgcgatgaac ttcgaaaact gcagaaaagg ttggggataa
     4501 ctagccttta tgtaacgcat gaccagagcg aggcaa
//
LOCUS       DAOMZL010000115         4515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_18099_length_4515_cov_18.707399, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000115 DAOMZL010000000
VERSION     DAOMZL010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18099_length_4515_cov_18.707399"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..859)
                     /locus_tag="PLQ30_07625"
     CDS             complement(2..859)
                     /locus_tag="PLQ30_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPW02355.1"
                     /translation="MSSFFQAVINPSIPFVRNALIGSVLAAFLFGILGSLVTVRRIAG
                     LAGAISHTVLGGIGLALFLVSRGAPEWISPMLGALVFAILAAITISIVSIKAKQREDT
                     VINALWAIGMSLGVIFMAKTPGYADPMSYLFGNILLVTQKNLYILGALNILVLLLVWR
                     YYRQIEAAFFDEEFAATKGLPVMVIYLVLLLVIAVAIVMLQTFVGIVMVIAMLTLPAG
                     TAGFFSRNLAKMMINSSILALVFSVLGLILSWVFDLPAGALIVLIAGMVYIGISVVRM
                     LQGRRSSTS"
     gene            complement(856..1746)
                     /locus_tag="PLQ30_07630"
     CDS             complement(856..1746)
                     /locus_tag="PLQ30_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW02356.1"
                     /translation="MNQEIKYRIASLRQRTGSLNSIEGKEHGASIEPTLPHPPDNVAI
                     WCHDLGFCYREAEVFKNVSFHVHSGEFVVLTGKNGAGKSTLLRLIVGLLEPQQGQVLV
                     FGVSPRRRIESIGYVPQHTNFDQAFPIRVEDVVGMGRLSGLSYISKDQKKVDIEWAME
                     QAEVTDLRARPFSALSGGQRRRVLVARALVGHPRLLVMDEPTANMDIGSENKFFEVLA
                     RIKRSTTVLIATHDSNFVSGLADTVLCVGQDSEHPHSVHRHAMEKAEDLPKDIYGSNM
                     LRVRHDIELPDNACCEGKDQ"
     gene            complement(1748..2689)
                     /locus_tag="PLQ30_07635"
     CDS             complement(1748..2689)
                     /locus_tag="PLQ30_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPW02357.1"
                     /translation="MKTHRYSNARRFLMRIILIIFLGIAGLGYSENKKQPIKVAVSIP
                     PMREWINRIAGDKVEAIVIMPPGSNPHSFEPSPRQLAELGNAKLWFSINVDFEYSLKP
                     KLRAMFPKLQIIDVTKDVQFRKLRPGEKEHDQDAVDKYAENSDPALVNLDQHTWLGIE
                     QAKTEIMVIQDTLIAIDPENRELYKTNCQDYLKDIDAVYSALKTKLAPLSGARVFVYH
                     PAFGYFLDMFDIEQVAVELGGKEPTQRELYALIELAKKEKACAIFTQVQFSESAARAI
                     ASAIGAVVVPIDPLASDWLENLSRIGQALSMSIGKRE"
     gene            2831..3325
                     /locus_tag="PLQ30_07640"
     CDS             2831..3325
                     /locus_tag="PLQ30_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPW02358.1"
                     /translation="MDELMQALRNALKSEVDSVTIYSEAALRGEGDVAQFFASRAEEE
                     KRHYNWLLSYYREMLAGQMPAMNLAAEIQAGSHKSPIFSHDFLKRIASDRHVSAAVSS
                     AVLLELNSTQYYSNMANLAKEPHLKAFFNSMSEWEMRHYEELLKIQEESKSYWFEVQN
                     FEPF"
     gene            complement(3499..>4515)
                     /locus_tag="PLQ30_07645"
     CDS             complement(3499..>4515)
                     /locus_tag="PLQ30_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPW02359.1"
                     /translation="AMMKYMTSIKDIARLAGVSPSTVSRVLNEREYVRDEVRQRVLSI
                     VKEKGYVQNNVARSMVLKRSFTIGVVIPHLFNMFQRQLFASMEFFLEQCGYKTQFFFI
                     RWSEASEQAFLRRLKSETLDGIIFIHELDSPAVYQHIQERGLPVALCTFEKEQFDFPA
                     VHIEEEASAALAIEYLIQKGHSNIGLITGGHFSFGKQREEGYRQALKAHGIPCNPELI
                     VRVPSYNPEAGRSGMITLLNRRSPMTAVFAATDELAIGAIKSLYDSGLRVPEDISVIG
                     LDDIDISAFTTPGLTTVHQPIFEMGKKSADLVCDWIARGSMQGKVELFSTTIVERESV
                     RALA"
BASE COUNT         1224 a         1094 c         1019 g         1178 t
ORIGIN      
        1 atcatgatgt cgacgatcta cgaccttgta gcattctgac aaccgaaatc ccgatataga
       61 ccatacccgc aattagaact atcaaggctc ctgccggcaa atcgaaaacc catgacagaa
      121 tcagcccaag cacagaaaag acaagggcta gaatactcga atttatcatc atcttagcta
      181 gattgcgaga aaaaaagcca gccgttcccg ccggcaaagt cagcatagca ataaccatga
      241 ctattccaac aaaggtttga agcatgacta tcgcgaccgc gatcacgaga agaaggacga
      301 ggtatatgac cataaccggc aggcctttag tagcggcaaa ttcctcatca aaaaaagctg
      361 cttcgatttg tctgtaatat cgccatacaa gcagcaatac taatatgttc agcgctccca
      421 gaatataaag attcttctga gtcacaagga gtatattacc gaagagatag ctcatgggat
      481 cggcatagcc cggagtcttg gccataaaga tcacaccaag gctcataccg atggcccaaa
      541 gcgcattgat gacagtgtcc tcgcgttgtt ttgcctttat cgacacgata ctgatcgtaa
      601 tagcagcaag gatggcgaat acaagcgcac caagcatggg acttatccac tcaggagcac
      661 ctctcgaaac aagaaagaga gcaagcccta tgccacccaa taccgtatgt gaaatcgcgc
      721 cagcaagccc tgcaatccta cgcacggtga ccagagaacc aagtatgcca aataaaaaag
      781 ctgccagcac gctgcctatg agagcatttc tgacaaaggg aatagaagga tttattactg
      841 cctggaagaa gctgctcatt ggtcttttcc ttcgcaacaa gcattgtccg gcagctcgat
      901 atcatgccga acacgaagca tgttgcttcc gtatatgtcc tttggcagat cttccgcctt
      961 ttccatggca tgtcggtgta ccgagtgagg atgctcggaa tcctgaccta cacagagaac
     1021 ggtatccgca agtcctgaca cgaagttcga atcatgagtg gcgatcaaaa ccgttgtgct
     1081 tctctttatg cgagccagca cttcgaagaa tttattctcg ctaccaatat ccatatttgc
     1141 agttggttca tccatgacaa ggagccttgg atgtcctact agcgctcgag ctaccaggac
     1201 tcttcgcctc tgtcccccgg aaagagcaga gaatggtcgt gctcgcaggt ccgtcacttc
     1261 tgcttgctcc atagcccact cgatatccac ttttttttgg tctttcgaaa tgtatgaaag
     1321 cccggagagc cttcccatac ctacgacatc ttcgactcga atgggaaagg cttggtcaaa
     1381 gtttgtatgc tgaggcacat agcctataga ttcaattcgt ctgcgaggag agacgccaaa
     1441 cacaaggact tgtccctgct gtggttccag aagacctaca atgagtctaa gcaaggtact
     1501 tttacccgct ccattcttgc cggtaagcac tacgaattcg ccgctatgta catggaagct
     1561 gacatttttg aacacttctg cttcgcgata acaaaagccc agatcatggc accagatggc
     1621 aacattatcc ggaggatgag gcagtgtagg ttcgatagaa gcaccatgtt ccttgccttc
     1681 tatggaattc aggctacccg tcctttgcct tagcgaggct atccgatatt taatctcctg
     1741 gttcattcta ttctcttttg cctattgaca tagacaaggc ctggcctatt ctggacaaat
     1801 tttctagcca gtcggaagct agagggtcga tcggcacaac cacggctcct attgcgcttg
     1861 caatggctct tgcagcgctt tctgaaaatt gcacctgagt gaaaattgcg caggctttct
     1921 ctttctttgc aagctctatg agagcgtaaa gctctctctg cgtcggttcc ttgccaccta
     1981 gctcgaccgc aacctgttca atatcgaaca tatcaagaaa atagccaaat gctggatgat
     2041 atacaaatac tcgagcaccg gaaagcggcg caagctttgt cttcagtgct gagtatacgg
     2101 catcgatatc tttcagatag tcttgacaat tggtcttgta tagctctcta ttttcaggat
     2161 ctatggctat caaggtatcc tgaattacca tgatctctgt cttggcttgc tcaataccaa
     2221 gccaggtatg ctgatcgaga tttacaaggg cgggatcgga attttctgca tatttgtcaa
     2281 ctgcatcttg gtcatgttcc ttctcgcctg gccgaagctt tctgaattgg acatcttttg
     2341 tcacatctat gatctgaagc tttggaaaca tggcgcgcaa tttgggttta agcgaatact
     2401 caaagtctac attgatggaa aaccatagct tcgcgtttcc caattctgcg agctggcgag
     2461 gcgagggttc aaaagaatga ggattcgagc ctggaggcat tatgacaatg gcttcaactt
     2521 tatctcccgc aatgcgattt atccattctc tcattggagg aatactgacc gctactttga
     2581 taggctgttt cttgttctct gaatacccta accctgcaat tcccagaaaa atgatcagaa
     2641 ttatgcgcat caaaaacctt ctggcatttg aatatcgatg cgttttcata gttccctccg
     2701 catatgcaag tcacttgcaa aacatatcga gaaaatagaa tttttgcaag tgatttgcaa
     2761 gaaatcgaaa atctcgcctc tagctccgat tttgaagtat tatatataaa aagagtaaat
     2821 ggaggttctt atggacgagc tcatgcaagc tttaagaaat gctttgaaaa gcgaagtcga
     2881 tagtgtgacc atttattcag aagccgccct gcgtggtgaa ggagatgtcg ctcaattctt
     2941 cgcaagtcgc gcagaagagg aaaaacgcca ttacaactgg ctcttgagtt attatagaga
     3001 aatgctagcc gggcagatgc cggcaatgaa tctagccgcg gaaatacagg cgggttcgca
     3061 taaaagcccg atattcagcc atgatttcct gaaaaggatt gcttcagatc gccatgtcag
     3121 tgctgcggtt tcaagcgctg tccttctcga actgaatagt actcaatact atagcaatat
     3181 ggctaatctt gcaaaggaac ctcacctcaa ggcattcttc aactcgatgt ctgaatggga
     3241 aatgcgacat tatgaagagt tattgaagat tcaagaagaa tcgaaaagtt actggtttga
     3301 agtgcagaac tttgagcctt tttgagagcc ttttcaggct cttttagatc tgagcttttt
     3361 cgaatgatct cggaatggcc tcatcggaac aggcccatgg gaaggcccct gtcggaaggg
     3421 cgcctcggaa gggttccatc ggaatggcac catcggaagg gccacaaggg cgccgatctc
     3481 cagaggatta aaatcactct aagccaatgc ccgtactgat tcgcgctcta cgatggttgt
     3541 tgagaaaagc tctacttttc cttgcatgct ccctctggca atccaatcgc atacaagatc
     3601 ggcgctcttc ttgcccattt cgaagatcgg ctgatggacc gtggtcaggc caggagtcgt
     3661 aaaggctgaa atatcgatat catcgagacc aattacactg atgtcttcgg gcactctaag
     3721 gcctgaatca taaagcgatt ttatcgcgcc tattgcaagc tcatctgttg cagcaaacac
     3781 cgcagtcata ggcgatcttc tgttcaagag tgttatcata cccgagcggc cagcctcggg
     3841 gttgtaggaa ggaacgcgaa caatgagttc tgggttgcaa ggaataccat gcgccttgag
     3901 cgcttggcga tagccttctt ctcgctgctt tccgaaacta aagtgtcctc ctgtaatgag
     3961 cccgatattg ctgtgtcctt tctgtataag gtattcgatc gccagggcag cggaggcttc
     4021 ttcttctatg tgaaccgcag ggaaatcgaa ttgctccttt tcgaatgtgc atagcgcgac
     4081 aggcaagcct ctctcttgga tatgctgata gaccgccggg ctgtctagct catgaataaa
     4141 tattatgcca tctagcgtct cgctttttag tcggcgcaga aaagcttgtt cgctggcctc
     4201 agaccatcga atgaaaaaga actgagtctt ataaccacat tgctcaagga aaaactccat
     4261 cgatgcaaag agctgtctct gaaacatatt gaacagatga gggataacaa cgccaattgt
     4321 aaagcttctc ttgagtacca tcgaccgcgc tacattgttc tgcacatatc ctttttcttt
     4381 gacgatgctg agcacgcgtt gacgcacttc gtcgcgcaca tattccctct cattaaggac
     4441 gcgcgaaacg gtagaaggcg aaacgccagc aagcctggcg atatctttga tactggtcat
     4501 atatttcatc atagc
//
LOCUS       DAOMZL010000116         4475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_18308_length_4475_cov_10.599774, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000116 DAOMZL010000000
VERSION     DAOMZL010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18308_length_4475_cov_10.599774"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..1732
                     /locus_tag="PLQ30_07650"
     CDS             311..1732
                     /locus_tag="PLQ30_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPW02360.1"
                     /translation="MNTTALIIVLIYLAAMLVVGFWTNKKLNKTSTDYMLAGRAVPML
                     IVACSLAANNIGGGSTNGLVNRAFGSWGISAFWYVMAASIGLIPMIFFAPKLRGVMAF
                     TIPEVVGRRFGKPSHIISAILNIIALFCLTASQILTSGTILSLLIGLDLNFAIVLAAA
                     FTIIYTTVGGLWADSITDVFQWIIIFLGLLIALPFAINAAGGWNTMMTALPAEKLEPF
                     GTLGFLGILSLVINYFLTFTAGPEMVSRIFSAKDAKASRNALIWAAVFMGVFSFVPTF
                     IGLAGYAIDPNMPSNQVLAQIIFGHTPAWVAGVVSAAIIASTMSSADSDMLCGSTIFT
                     KDLLPYFKKDVPDKTQIVISRILNVIIGLGAMCIALFKIDIITLNVFSFMLRAAGPIA
                     AFLLGLLWKNAGKSAGIVSIIIGSLVGIGWQILANQMKVDNPYGFLPVVVGSAASFLT
                     FIVSTYIERAMGRPPAPMLDIKE"
     gene            1811..3028
                     /gene="ygeW"
                     /locus_tag="PLQ30_07655"
     CDS             1811..3028
                     /gene="ygeW"
                     /locus_tag="PLQ30_07655"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HPW02361.1"
                     /translation="MSKKDIEKFIDELRTKKISMYQKDFLLTWEKSLPEIQATFLVAD
                     ILRAMRESNISPKVWDSGIAVSNFRDNSTRTRFSFASACDLLGLYVQDLDEGKSQVAH
                     GETVRETATMISFLTEAIGIRDDMYLGEGDRYQREVGTALDDGVKAGVLPSRPTIVNL
                     QSDIDHPTQAMADFLHLVHYFGGLDKLKGKKITMSWAYSPSYGKPLSVPQGIIGLASR
                     FGMNIVLAYPKGYDLIPDVVELAKKQAIDSGGSFTYVNSMDEAFKDANIVYPKSWAPY
                     DVMLRRVPLLHAGDKEGLKELEREALANNAKFKDWECTEEKMKLTRSAAPNSSQALYM
                     HCLPADITGVSCAAGEVAASVFDRYRDATYREASFKPYIIAAMILLGKFERPAELLES
                     LLTRGRQRTYGNF"
     gene            complement(3199..3627)
                     /locus_tag="PLQ30_07660"
     CDS             complement(3199..3627)
                     /locus_tag="PLQ30_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02362.1"
                     /translation="MKRSIAISIIVCLVVLSAKAQVITFGLGDAELEAGLNELNISAK
                     VNMQVFQTEVSMVWGQPTASITFALSQGLTPGEVYLAAALAHLSSKPLNIVVELYQKN
                     KQKGWGALAKDLGIKPGSKEFKQLKEQLAGSSKKLKSQKK"
     gene            complement(3886..>4475)
                     /locus_tag="PLQ30_07665"
     CDS             complement(3886..>4475)
                     /locus_tag="PLQ30_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW02363.1"
                     /translation="PRAGSVNLDSRPLISYSARERSQLIAFLPQLEKLAFNYRVMDFV
                     LMGRTPRMEVLALPGLEDEEAALQALSLCKLDSFSMRNIGELSGGEFQLVRIARCLAQ
                     GASILILDEPASMLDPAHSRHIADVLASLADSGITIIYTTHDIELGLFLGGEALILSG
                     GMIQWSGPAEGLHNTEILTRTYGISFSNKEIPSAF"
BASE COUNT         1158 a         1130 c         1053 g         1134 t
ORIGIN      
        1 aagaagatgc aaatatggtc gctcttcgat tatacaaaaa ggcaggaata gcatagtgct
       61 aaaatcgtgg cagcctggca taggccggcc ttagcataga ccagctttag tgtaggctcg
      121 caaccgcaga gacgtatgca gtatggagga ctcgagcgtc gcaagccgga gaaacgccag
      181 ctcgaataga gcgagcccaa gtctagtaga cctttagcaa acaatgggct agctatgatt
      241 attgaggcga tttctgagta agcgtcactt actcagagag cacatttcat aaggataagg
      301 gaggtaccgt atgaacacaa ctgcgctcat catcgttctc atttatttgg cagcaatgct
      361 tgtggtaggg ttttggacaa acaaaaaact caacaaaacg agcaccgact acatgctcgc
      421 gggtcgggcg gtgccgatgc tcatcgttgc ttgcagcctc gcagcgaaca acatcggcgg
      481 aggatcgacg aatggactcg taaatcgggc ttttggctcc tggggcatat ctgcattttg
      541 gtacgtgatg gcagcatcca taggtctcat cccgatgatt ttctttgccc ctaagctccg
      601 cggagtcatg gctttcacaa ttcctgaagt ggtagggcgg cgttttggca agccatcgca
      661 tatcatatca gcgattctca atatcatcgc gcttttctgc ctcactgcat cgcagattct
      721 cacatcgggt acaattctct ccttgctaat cggactcgat ctcaacttcg ccatcgtgct
      781 cgctgcagca ttcacaatca tctataccac ggtaggaggg ctctgggcag attcgatcac
      841 cgatgttttc cagtggatca tcatctttct cgggctgctc atcgctctac cctttgcaat
      901 caatgctgct ggtggctgga acacaatgat gactgctctc cctgctgaga agcttgagcc
      961 ttttggaacc ctcggctttc tcggtatttt atcgctcgtc atcaactatt tcctcacttt
     1021 tactgcaggc cctgagatgg tctcccgcat tttttcagcc aaggatgcaa aggcaagccg
     1081 taatgcgctc atttgggccg ctgtttttat gggggtattc tcttttgtgc caacattcat
     1141 tggccttgct ggatatgcga tcgatcccaa tatgccaagc aatcaggtgc tcgcacaaat
     1201 tatctttggc cacactcctg catgggtagc tggtgtcgtc tcagcagcga tcatcgcatc
     1261 gacgatgtcg agcgctgatt cagatatgct ctgcggatcg acgattttca cgaaagattt
     1321 gttgccctat ttcaaaaagg atgtcccgga taagacacag atcgtcattt cgcgcattct
     1381 caatgtgatc attggcctcg gggcgatgtg cattgcgctt ttcaaaatcg atatcatcac
     1441 tctcaatgtg ttctcgttca tgcttcgcgc tgcaggccca atcgctgcct tccttcttgg
     1501 gttactgtgg aagaatgctg gtaaatctgc cggcatcgtc tccatcatca ttggctcgct
     1561 cgtcggaatt ggttggcaga ttcttgcgaa ccaaatgaag gtcgacaatc cctatgggtt
     1621 cttgccagtc gttgtaggct cagcagccag ttttctcacc tttattgttt caacatatat
     1681 agaacgagcc atgggacggc cgcctgcccc tatgcttgat atcaaagaat agatcgacga
     1741 gtcaatcgac gataacattg aataatatta gctaagaatt gttggaggat ttttaatgga
     1801 acaccattca atgagcaaaa aagacataga aaaattcatc gacgaacttc ggacaaaaaa
     1861 gatttctatg tatcagaaag attttcttct cacgtgggag aagtccctcc cagaaatcca
     1921 ggccaccttc ctcgtggccg atatccttcg cgcaatgaga gaaagcaata tttcgcctaa
     1981 agtctgggac tcaggcatcg cggtgtcgaa cttccgcgac aattcgactc gcactcgctt
     2041 ttcatttgca tctgcatgcg atctgctcgg tctctatgtg caggacctcg atgaaggcaa
     2101 aagccaggtc gcccatggcg aaacagtgcg cgaaacagcc accatgatca gcttcctcac
     2161 tgaagcaatt ggcatacgcg acgacatgta ccttggagaa ggcgaccgct atcagcgcga
     2221 agttggtact gcgctcgacg atggcgtcaa ggcaggtgtg ctgccttcca ggcctacaat
     2281 cgtcaatcta cagagcgata tcgaccatcc gacacaagct atggccgatt ttttgcacct
     2341 tgtgcattac tttggaggcc tcgacaagct caaaggcaaa aagatcacga tgagctgggc
     2401 ttacagcccc tcttatggca agccgctgtc cgtgccacag ggcatcattg gtcttgcaag
     2461 ccgctttggc atgaacattg tactcgccta tccaaaggga tacgatctaa tccctgatgt
     2521 cgtcgaactg gctaaaaagc aagcgataga ctcgggcggc agttttacat atgtcaattc
     2581 gatggacgaa gcattcaaag atgccaatat tgtgtatccc aaaagctggg cgccatacga
     2641 tgttatgctc cgccgcgtgc cccttctcca tgctggagat aaagaaggct tgaaagaact
     2701 cgaaagagaa gctctcgcca acaatgcaaa attcaaggac tgggaatgca ccgaagaaaa
     2761 gatgaagctc acaaggagcg cggcaccaaa ttcctcacag gcgctctata tgcattgttt
     2821 gccagcagat ataacgggtg tctcctgcgc tgccggcgaa gtcgccgcta gcgtgttcga
     2881 tcgctaccgc gatgcgacct atcgcgaagc gagctttaag ccctatatca tcgccgcgat
     2941 gattctttta ggtaaatttg agcggccggc tgagctactc gagagcctct tgactagagg
     3001 cagacagagg acttatggca acttctgatt ctagggtcta agaatactag agcaccgagc
     3061 ttatctagag tctacttgct tttctcacag tgatacagag agcgatacta caagtgaccc
     3121 taaaatgcaa agaaagcgaa ggaggctgcc tagaatggcg ccgctttttg agttttcact
     3181 accctatata aagtcccatc actttttttg cgatttcagc ttcttgctag agcctgcaag
     3241 ttgttccttc agctgtttga attccttgga acctggcttt atgccaagat cctttgcaag
     3301 agcgccccag cctttttgtt tattcttttg atacagctca accacaatgt taagcggctt
     3361 actagaaaga tgggccagtg ctgcagcaag atatacttcg ccaggcgtaa gcccttgcga
     3421 taacgcgaaa gtgatgctcg ctgtgggctg tccccagacc atggagactt ctgtctgaaa
     3481 gacctgcata ttgacttttg cacttatatt gagctcattc aagccagctt caagctcagc
     3541 atcaccgaga ccaaaagtga tgacttgcgc tttagcgctc agtactacta aacatacgat
     3601 aatggaaatt gcaatcgatc gcttcataga acaccccttt ttcaaatata ctatggcaaa
     3661 agcgcaccaa caatatgaac tataataaat gccgatagtt gcgcccatta gtatgtcata
     3721 gtctagaccg ggcttgagcc tcttcgatga gataataatg gagcacgact gcctgattga
     3781 attcggtatc tcgagacgca aaaagcagag tgatcctgcc cttgcgtgcg cacaggttta
     3841 tagattcgat tcatcataat atcaagcata gctgctccat atgcctcaaa atgcactcgg
     3901 tatctctttg tttgaaaaag agataccgta agtccgggtg agaatctcag tattgtgtaa
     3961 gccctcagca ggcccactcc actgaatcat gcctcccgaa agtatcagcg cttcccctcc
     4021 aagaaaaagg cccaattcga tatcgtgcgt tgtatagatt attgttatac ctgaatcggc
     4081 gagcgatgcg agcacatcag cgatatgccg cgaatgcgct ggatccagca tgcttgctgg
     4141 ctcatccaga attagaatag aagccccctg agcaaggcaa cgcgcgatcc gcacaagctg
     4201 aaactcgcca ccggaaagct caccaatatt gcgcatgcta aaggaatcga gcttgcaaag
     4261 agaaagcgcc tgcaatgcgg cttcttcatc ctccaatcca ggcaaggcca gaacttccat
     4321 gcgaggcgtc cgacccatca atacaaaatc cattactcga taattgaaag cgagtttttc
     4381 gagttgtggc aaaaatgcta tcaattgcga gcgctctcta gccgaatagg aaataagcgg
     4441 gcgactatcg aggttgacgg agccggcgcg cggac
//
LOCUS       DAOMZL010000117         4344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_19069_length_4344_cov_7.096759, whole genome shotgun sequence.
ACCESSION   DAOMZL010000117 DAOMZL010000000
VERSION     DAOMZL010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19069_length_4344_cov_7.096759"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="PLQ30_07670"
     CDS             complement(<1..1093)
                     /locus_tag="PLQ30_07670"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HPW02364.1"
                     /translation="MRKLEKSAALWNKARPRFLKKIIIAIIVLFIALSSLSSQDIFTP
                     SAGAGSAGAGAQEIRPTIASSTEKLDVVGSVDPLPPEIPGLIPIATFDEGRSYRTVVG
                     SYSVLSLKGSWREMGRQYGALLSRELKEFYAEIIADLQARGLGQEHVEGVQATFQTYM
                     PEMKQLIEGMSETSGLSFEEHIMLDASFYILPDLVIEAAQAMASCSGIAVSSPRTADG
                     KLYFARNWDMTQAAMKPYLRYMALIVFNPTDGGLSFANVRPIGQVYVETGINEKGVFV
                     ELNNGSASDPNINENAVFSVASLFDFLRTSETLDEMIQNIVTTKMDASYIIQAANAQR
                     AVSIEKPTFDARVIEQQNGALYALNNFARP"
     gene            1271..1558
                     /locus_tag="PLQ30_07675"
     CDS             1271..1558
                     /locus_tag="PLQ30_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02365.1"
                     /translation="MLSARAAPAASPQAPAQASQFEKASTSASHISFSFSIVFGGYGH
                     FGFEFSMPNTSGSVSLSYFIELGTNGIGAKTNSKDTYLNGFAVSTGLRVYL"
     gene            complement(1645..2055)
                     /locus_tag="PLQ30_07680"
     CDS             complement(1645..2055)
                     /locus_tag="PLQ30_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPW02366.1"
                     /translation="MKYLFDTDIISSLMKGKASGRLLSRMEGAGRSNQAISSITVFEI
                     VYGAYHTSSPQRFLGLFEQKVLPFVDILAFDETAARLAGRIRAEREALGKPIAPMDLQ
                     IAAIALATGRILVTGNTKHYADIGSLDIENWLEG"
     gene            complement(2039..2314)
                     /locus_tag="PLQ30_07685"
     CDS             complement(2039..2314)
                     /locus_tag="PLQ30_07685"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HPW02367.1"
                     /translation="MADILVPVHELKAKLSEYLGRTLHRKERIIISRRNRPIAMIIPV
                     DQEGMQKDVTDSSGLEKVDWSGFSELADSVEAVYEGRQGEGYREVSF"
     gene            complement(2579..3226)
                     /locus_tag="PLQ30_07690"
     CDS             complement(2579..3226)
                     /locus_tag="PLQ30_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HPW02368.1"
                     /translation="MSVITFSREPCSGGTQIAQSVARKLGYRFATKETIEQVLLQYGF
                     VDVEEAYDSIPNFWTRFDERTHQIVAMFDRVIVALAKLGDVVIVGRGAFKVLASYRDV
                     LNVRIKAPFPQRVATYKQRYNISDQRGAENAVIEADRIRTAFLEVHYGIKWDSMEHFD
                     LVIDTCKITPRTAIPMVIEANKAVMSAICEPSECASSIAVDDILLEATLKVIEER"
     gene            3435..>4344
                     /locus_tag="PLQ30_07695"
     CDS             3435..>4344
                     /locus_tag="PLQ30_07695"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPW02369.1"
                     /translation="MSQTERIFYIDRIIREHGGISTHQVCEKFEVSSRQAKRDIEYLR
                     NRLDAPIVWSRRRMRYEYSEPWNSLKFADETSFFALAFLKAILNRYAYVPVVSEETMK
                     LLEEKIAGRYGAISNRVRYELPDMEIIDGEVAYTMCQALLDNSLLEISYTDAKGEWSV
                     RRIVPLRLVNYAGKWYCIAFDEKTMQMRTFSISRIADVRPAGLPNYVLPESDAIERYL
                     SSSYGIFKGDVLGTATLRFFGGAARAVKIQQWHKDQQITLVTGVGDPDAIDLSIPVHD
                     WIEILGRALRCGSNCEVLGPPEFRKLW"
BASE COUNT         1148 a         1076 c          990 g         1130 t
ORIGIN      
        1 tagggcgggc aaagttattg agcgcgtata aggcaccatt ttgctgctcg ataacgcgag
       61 catcgaaggt gggcttctcg atagagacag cgcgttgggc atttgcggcc tggataatgt
      121 atgacgcatc catttttgtc gtgacaatgt tctggatcat ttcgtcgagc gtttcgctcg
      181 tacgaaggaa atcaaaaaga gaggccaccg aaaataccgc attctcattg atatttgggt
      241 ctgaagccga accattgttg agttcgacaa acacgccttt ttcatttatt cctgtttcga
      301 cataaacctg tcctattggt ctcacattag cgaaggacaa tcctccatca gtaggattga
      361 aaactataag cgccatatag cgaagatagg gtttcatagc ggcttgggtc atgtcccagt
      421 tgcgcgcaaa atagagcttt ccgtcggccg tccgggggct ggagacggca attcccgaac
      481 acgaggccat agcctgtgcc gcttctatga ccaagtcggg caatatataa aaagaggcgt
      541 ccagcattat gtgctcttca aaagataaac ccgaagtctc agacattcct tctataagct
      601 gcttcatttc tggcatgtag gtttggaatg tagcttgtac gccttcgaca tgttcttgtc
      661 ccaagccacg ggcctgcaga tcggcgatga tctccgcata gaattctttg agctcccttg
      721 agagcagcgc cccatattgt cgacccatct cgcgccagct tcccttgagg cttaatacag
      781 aataagagcc aaccacggtt cggtagcttc ttccttcatc aaatgttgct atcggaatta
      841 gcccagggat ttctgggggc agtggatcga cagagccgac aacatccagc ttttccgtcg
      901 aagaagctat ggtggggcgg atttcttgag ccccagcacc tgctgatcca gcacctgccg
      961 atggagtgaa gatatcttgt gaagatagag agctcagcgc aatgaacaac acaataattg
     1021 ctatgatgat ctttttgaga aaccttggtc gtgccttatt ccataaagcc gccgattttt
     1081 ccagctttct cattgttttc tccttagaat actccctaga atattcctta gaatgctcag
     1141 caattagaag gatacatcaa aaaactatac catggctttc gcatcaaaga tagcttagga
     1201 atttgctgta caaccgctcc ctggcacagg agctatagag aatccgtata gctatcaaaa
     1261 ctgcgacgag gtgctctcgg cccgagccgc tcccgcggca tcaccccaag cgcccgctca
     1321 agcttctcaa tttgagaaag catccaccag cgcgagccat atctcgttca gcttttctat
     1381 tgtgttcggt ggctatgggc attttggctt tgaattctct atgccgaata ccagcggttc
     1441 ggtatcattg tcgtatttca tcgagctcgg cacgaatggc ataggcgcaa agaccaatag
     1501 taaagacacc tatctcaacg gctttgcagt gagcactggc ttaagggtat atctatagag
     1561 attattcggt ccaatgggca ccgcgtgccc tttagaaggc gatagtacca ttaggcagca
     1621 ttaagaacaa agcatagctc ctgtttatcc ctcaagccaa ttttcaatat cgagtgaacc
     1681 gatatctgca taatgcttcg tattgccagt aacaagaatc cttcctgttg caagggctat
     1741 tgctgcaatt tggagatcca taggagcgat tggttttccg agagcttcgc gttcggcccg
     1801 tatccgacca gcgaggcgcg ccgctgtctc atcgaatgca aggatatcga cgaagggaag
     1861 cactttttgt tcgaagagac ctaaaaacct ttgtggcgac gaggtatgat aggcaccata
     1921 tacaatttca aatacagtga tcgaagaaat agcctgattt gatctgcctg caccttccat
     1981 tcgggatagg agccggcccg atgctttgcc tttcatcaat gaggaaatga tatcagtatc
     2041 aaaaagatac ttcacgataa ccctcgcctt gtcttccctc atacaccgct tcgaccgaat
     2101 cagcaagctc ggagaatccg ctccagtcca ctttttctaa tccgctcgaa tcagtaacat
     2161 ccttctgcat accctcttga tcgacaggaa ttatcattgc gatgggtcta tttcttctgg
     2221 aaataataat tctttccttt cgatgcagag tccttcctag atattccgag agcttcgctt
     2281 tgagttcgtg aacaggaaca agtatatcag ccataatagt ctcccataaa atagtcatta
     2341 tgactatttt atcaccttct tcgcacaatc ggcaagacgt tactacagag atcatacccg
     2401 gcctaaagct ttcattccct tacccgcaga aggaaaggat aatccaccat tcaaattgac
     2461 aatcttctgt acacctctcc atattgagca accctttaat aagagttttg cttattattg
     2521 gaaatagcca aaacgtttaa tagcgctaga ctaacaaaat gtcctgtatg gtagctgtct
     2581 atcgttcttc aatcaccttg agcgtcgctt cgagcagaat atcatcgacc gcaatggatg
     2641 aggcgcactc gcttggttcg catatagcgc tcatgaccgc tttgttggct tcgatcacca
     2701 taggaattgc agtgcgtggg gttatcttgc aagtatcgat gacgagatcg aaatgttcca
     2761 tcgaatccca tttaatacca tagtgaactt ccaagaaggc ggttctgatc ctgtctgctt
     2821 caatgacagc attctccgcg ccacgctgat ctgaaatatt ataccgctgc ttataggtcg
     2881 cgacccgctg cggaaatggc gccttgatgc gtacattgag cacatcgcga taggaagcga
     2941 gcaccttgaa agcgcctcgg cccacaatca ccacatcgcc gagttttgca agcgccacaa
     3001 tgacacgatc gaacatggcg acaatttgat gcgtccgctc atcgaagcgt gtccagaaat
     3061 tgggaatgct atcgtaggct tcctcgacat cgacaaaacc atattgcagc agtacttgct
     3121 cgattgtctc ttttgtagca aatcgataac caagcttccg tgcaaccgac tgcgcaatct
     3181 gagttccccc tgagcaaggt tctctcgaaa aagtgatcac actcacagcg tgcctcctgc
     3241 gttatttaat tcttctctct acataaagta ttggccaaaa aacaggtgga gtcaaggaaa
     3301 agaaatcccc aaaggaatcc gccaatataa aagcgcatta aagcgattat aatgtcgata
     3361 attattttga gggactcttg gtgtccctcc caaggagtat aatcaaaaat aaaaaattgc
     3421 gggtggaggc ctccatgagc caaaccgaaa ggattttcta catcgatcgc atcataaggg
     3481 aacatggagg catctccact catcaagttt gtgaaaaatt cgaggtctcg agtcgccagg
     3541 ctaagcgcga tatagaatat ctgcgcaata ggctcgatgc accgatagtc tggtcgcgtc
     3601 gtaggatgcg atatgaatac tctgagccgt ggaatagcct aaaatttgca gatgagacat
     3661 cgttctttgc acttgccttt ctgaaagcaa tccttaaccg atatgcctat gttcctgtgg
     3721 tatccgagga aacgatgaag ttgctcgagg agaaaatcgc tgggcgctat ggagcgatct
     3781 ccaacagagt ccgctacgag cttcctgaca tggaaatcat cgatggcgag gtcgcgtaca
     3841 cgatgtgcca ggctctctta gacaatagtc tactcgaaat ctcctatact gacgcgaaag
     3901 gcgaatggag tgtgcggcga attgtaccgc tacgactcgt caattatgcg ggcaaatggt
     3961 actgcatcgc ttttgatgaa aaaactatgc agatgagaac tttctcaata tcgcgcatag
     4021 cagatgtacg gcctgcaggc cttccaaact atgtgcttcc ggagagcgat gcaatcgaac
     4081 gctatctttc gtcatcatat ggcattttca aaggcgacgt gcttggcaca gcaaccctgc
     4141 gcttctttgg gggtgcagct cgcgcggtga aaatccaaca atggcataag gatcaacaga
     4201 ttacccttgt gacaggtgtc ggcgaccccg atgccatcga cctctccatc cctgtgcacg
     4261 actggatcga aattcttggc cgcgctcttc gctgcggttc aaactgcgaa gtgctcggac
     4321 cgccagaatt ccgaaagctg tggg
//
LOCUS       DAOMZL010000118         4316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_19245_length_4316_cov_15.096691, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000118 DAOMZL010000000
VERSION     DAOMZL010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19245_length_4316_cov_15.096691"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..882)
                     /locus_tag="PLQ30_07700"
     CDS             complement(130..882)
                     /locus_tag="PLQ30_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW02370.1"
                     /translation="MNLLEIKNITLLKESRPILNNLSMEIWNGHVHAIIGPNGAGKST
                     LANTIMGLSGYRDFQGDVIFNGESLKDLSIDERARRGITLLFQEPARFEGLSVSRFIL
                     AGAKEKSQKVVEDALRQVGLVPENYINRAVDKTLSGGERKRIELASIYAMKPRLVLMD
                     EPDSGVDIDSVKYIFSIIKDLKQAGSTVILITHSPEVLKRADHAFLICAGTLVDKGDV
                     SRMFDYFSGKCVPCPHKGQPDTDRLVPMFAAN"
     gene            complement(1034..1555)
                     /locus_tag="PLQ30_07705"
     CDS             complement(1034..1555)
                     /locus_tag="PLQ30_07705"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPW02371.1"
                     /translation="MNRQDIPLKDFHYDVAKLRDHWFALFAGDFNARVFNAMTISWGS
                     YGHVWDKMVFQVMVRPSRYTYDFMNRYDSFTLNAFTPEYKRVLSILGSNSGRDIDKIA
                     LAGLTPMASRKVDAPSFEEAQLVIECRKIYWHDLNEANFLDSDIEEHYPRKDYHRIYL
                     GEIVNVSQQIIKP"
     gene            complement(1596..3503)
                     /locus_tag="PLQ30_07710"
     CDS             complement(1596..3503)
                     /locus_tag="PLQ30_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW02372.1"
                     /translation="MAYETSPSIQDVRTSFQRRGGRPTRYERASDPRKAMKRLFGYLM
                     PFRQPLLLIVFFVLLQSLLGLVGPYLLGSAIDKSIGGKDINGLARNAVLMLIAYFLSN
                     SFNLIANRKMAIISQNALKNLRRDLFVHIQWLPMRFFDTNPAGGLMSRLTNDIDAINQ
                     AVSQNIISLVAGVITMSGILVSMFVLNHWLALTSLIVIPIMYFFSNFVAKYTRKGFRE
                     LQKELGDLNAIAEETISGFKVIKAFRRNDSAIETFKRKNEAVYKAAIYANSYALLLMP
                     LTGVIGSFFVILLASLGGWLALKDLVSIGMIATFINYAQNFTQPLRQISNLYNSIQAA
                     LAGAERVFEIIDMDPEIAEEPRLEEAKTNPETAIRVSEDSIIGSSTAPMVFSLGQFQG
                     NVSFKNVVFGYLPGKPVIKNFNVDIPAGLTVALVGPTGAGKTTITNLLTRFYEIDEGQ
                     ILFDGVDIRTIPKEVVRRAIGLVLQDTFLFADSVLENIRFGRLDATDEECIEAAKMAE
                     VDHFIRQLPEGYQTNLSERASNLSQGQRQLLSIARAILADPAILILDEATSSVDTRTE
                     IRIQRALLRLMHGRTSIVIAHRLSTIRDADKIIVIDDGKIVEIGDHQALLAKRGFYHR
                     LYMSQFKGNEI"
     gene            complement(3493..>4316)
                     /locus_tag="PLQ30_07715"
     CDS             complement(3493..>4316)
                     /locus_tag="PLQ30_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW02373.1"
                     /translation="NEVLDAEPEVAELPNASSLPQTINGEVEFDHVYFHYNGDTALKV
                     LEDICTRAEARKVTAVLGATGSGKSSLVNLIPRFYDARSGKVNIDSNDVRLLRISSIL
                     EHIAIVPQETVLFSGTVRDNIRYGKPDASDEEVLAAAVAAHANDFIMDMPEHYETHIE
                     ERGANLSGGQKQRIAIARALITKPSILILDDATSAIDVETETKIHAAIAKATMGITLI
                     MVAQRISTVLNADKIIVLDEGRIVAEGTHRELLKKSPIYKEIYDSQLGAGVRHGL"
BASE COUNT         1125 a         1009 c          949 g         1233 t
ORIGIN      
        1 aagacctcat tgccatggat ttctatatgt gccacatctg gagcgaattt atgttgattg
       61 atcgatgcca gcagctctgc gataagaatg tctttatcta tgattgattg ctctgccatg
      121 gttttcctct caattcgccg caaacatagg aactagccta tccgtgtcgg gctggccttt
      181 atgtggacaa ggcacacact tgccactgaa atagtcgaac attctagata cgtcgccctt
      241 gtcgacaaga gttcctgcac agatgagaaa agcatgatct gcgcgcttca ggacctcagg
      301 gctatgggtg atgaggatga cagtggagcc tgcctgtttt agatctttta taatgctaaa
      361 gatatacttt acagaatcta tatctacacc ggaatctggc tcatccataa gcacaaggcg
      421 aggcttcatc gcatatatcg aagctagttc gatgcgcttt cgttcgcctc ccgagagtgt
      481 cttatccacg gcgcgattga tatagttttc tggtacaagt cctacctgtc gaagcgcatc
      541 ctcgacgacc ttctgagatt tctctttggc tcctgcaagt atgaatcggc tcacggacag
      601 cccttcgaag cgggcaggtt cttggaaaag caatgtaatt cctcttcgtg cccgctcatc
      661 tatcgaaaga tccttaagag actcaccatt aaaaatgaca tcaccttgaa aatcgcgata
      721 accggatagt cccattatgg tattagccag agtggactta cctgctccat taggaccaat
      781 gatcgcatgg acatgtccat tccagatctc catgcttaga ttgttcaaaa tgggtcttga
      841 ttctttgaga agcgttatat tcttgatttc gagaagattc ataattttgc cgtcccgtgt
      901 ctttggctga gctgcattat gctctgaata aaattcatat cattaatgta ggattttcct
      961 ttgagaaaaa tactcctttc caaaaagaaa atcaagtatg tggcagcaaa ccgaatattt
     1021 agagcggcaa accctatggt ttgattatct gctgactaac attgacaatc tcgcctagat
     1081 agatccgatg atagtccttt ctggggtaat gctcttctat atcactatcc aggaaattag
     1141 cttcatttaa gtcatgccaa tagatttttc ggcattctat tacaagctga gcttcctcga
     1201 aacttggggc atcaaccttg cgggaagcca taggagtaag accggccagt gctatcttat
     1261 caatgtctcg gcctgaattc gaaccaagaa tcgatagaac tcgtttgtac tctggagtaa
     1321 aagcgtttag agtaaagctg tcgtatcggt tcatgaagtc ataggtatat cttgagggac
     1381 gtaccataac ctgaaacacc atcttatccc atacatgccc ataactgccc caggaaattg
     1441 tcatcgcgtt gaagacgcga gcattaaaat cacctgcaaa taacgcaaac caatggtcgc
     1501 gtagttttgc tacatcgtag tgaaaatctt tcagcggtat atcctgccga ttcatgcttt
     1561 ttcctccagt gttttgatat atctatatga ttcgattata tttcatttcc cttgaattga
     1621 ctcatgtaaa gacgatgata aaagccgcgc tttgcaagga gcgcttgatg gtctcctatt
     1681 tccacaattt tgccatcatc tattacaatg atcttgtcgg catcgcgaat agtgctgagc
     1741 ctatgggcga taactatgct tgttcttccg tgcatgagcc tcaagagagc cctttggatg
     1801 cgaatttctg tgcgtgtatc gacgctgctt gtggcttcgt ccaatatgag tattgccgga
     1861 tccgctagaa ttgcccgcgc aatggataaa agctgacgct ggccctggct taaattgctt
     1921 gcacgctccg aaagatttgt ttgataacct tcgggaagct gtcgtataaa gtgatcgact
     1981 tccgccatct tagcagcttc gatacattcc tcgtccgtcg catccaatcg gccaaagcga
     2041 atattctcca gtaccgaatc ggcaaagaga aaagtatcct gcaaaaccaa acctatggct
     2101 cgccgaacta cttccttcgg aatagtgcgt atatccacac catcgaatag aatctgtcct
     2161 tcatcgatct cgtagaaacg cgtgagaagg ttagtaatcg tagtcttacc ggctcccgta
     2221 ggtccaacta acgctaccgt aagccccgca gggatatcta cgttgaaatt cttgattacg
     2281 ggtttgccgg gtaaatagcc gaataccaca ttcttgaagc tgacattgcc ctgaaattgt
     2341 ccgagactaa agaccattgg agccgtggaa gaaccgatta tagaatcttc gctgactctt
     2401 attgcagtct cgggatttgt tttggcttct tccagtcttg gttcctctgc tatctctgga
     2461 tccatatcta ttatctcgaa gactcgctcc gctccagcta gagcggcttg tatcgagttg
     2521 tatagatttg atatttgcct tagaggctga gtaaaattct gtgcatagtt tatgaatgta
     2581 gcaatcatgc caatgctcac aagatccttg agcgccaacc agccaccaag gctagcaagc
     2641 aatataacaa agaaactacc tatcacacct gtaagaggca ttagaagcaa tgcatatgaa
     2701 tttgcatata tagctgcttt atatacagct tcattttttc ttttgaaggt ttcgattgcg
     2761 gagtcatttc ggcggaaggc ttttatgacc ttaaagccgg aaatggtttc ctcggctata
     2821 gcgttgagat caccaagctc tttctgcaat tccctgaatc ccttacgagt atattttgcc
     2881 acaaaattgc tgaaaaaata cattatcggg atgacgatga ggcttgtaag cgctagccaa
     2941 tggttgagaa cgaacatact caccagaatg ccggacatcg taataacacc cgcgacgagt
     3001 gaaatgatat tttgtgatac agcctgattg atggcatcga tgtcattcgt tagcctgctc
     3061 atgagcccgc cagcggggtt cgtatcgaaa aagcgcattg ggagccactg gatatgcaca
     3121 aacaaatcgc ggcgtaggtt tttaagggca ttttgggata tgatcgccat ttttcgattt
     3181 gcgatcaaat taaagctatt gctcagaaaa taagcgatga gcataagaac agcatttcga
     3241 gctaatccat ttatgtcttt gccgccgatg ctcttatcta ttgcgctgcc gagcaaataa
     3301 ggaccgacta atccaaggag agattgcaga agcacaaaga acacaatcaa aagcagaggt
     3361 tgcctgaatg gcattagata tccgaagagc cttttcatcg ctttgcgagg atccgaggct
     3421 ctctcatatc ttgtaggcct gcctcctcgc ctttgaaagc tggtgcgcac atcctgaatc
     3481 gagggacttg tctcataagc catgacgcac ccccgcaccc aactgggaat cgtatatttc
     3541 tttgtagatt gggcttttct ttagaagctc tctatgagtg ccttctgcaa cgattcgtcc
     3601 ttcatcgagc acgattatct tgtctgcgtt aagcactgta ctgatgcgtt gagccaccat
     3661 gatgagggtt attcccatag tggccttcgc tatggcagcg tgaattttgg tctctgtttc
     3721 cacatctata gcgcttgttg cgtcatccaa aatgagaatc gaaggcttgg taataagggc
     3781 acgagctatg gcaatacgct gtttttgtcc gccagagaga ttagccccac gttcttcgat
     3841 gtgcgtctcg taatgctctg gcatatccat gataaagtcg ttggcatgag ccgcaactgc
     3901 ggcggcaagg acctcttcat cgcttgcatc gggctttcca tatcgaatgt tatcgcgcac
     3961 tgtgcccgaa aagagcacag tttcctgagg aactatggct atgtgttcga gaatcgatga
     4021 aatccttagg agtctaacgt cattggaatc gatattgacc tttcctgagc gagcatcata
     4081 gaaacgcgga atcaggttga caaggctgga ttttcccgaa cctgtcgcac ccagtacggc
     4141 ggtaaccttt cttgcttcgg ctcttgtaca tatatcctcc agcaccttaa gggctgtatc
     4201 tccattgtaa tggaaatata catgatcgaa ttcaacctct ccgttaatag tctgcggaag
     4261 cgaagaggcg ttgggcagct ctgcaacctc tggctcggca tccagcacct cattga
//
LOCUS       DAOMZL010000119         4284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_19446_length_4284_cov_15.874911, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000119 DAOMZL010000000
VERSION     DAOMZL010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19446_length_4284_cov_15.874911"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            441..1001
                     /locus_tag="PLQ30_07720"
     CDS             441..1001
                     /locus_tag="PLQ30_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02374.1"
                     /translation="MKKLLIVVFSMVSSLCLFPQPMENSVNYVSILLKNEAYLGIKSI
                     DWRYENNQYVFTLALNNALLFEKLTNKNTFIEWDIFIDIDSNTKTYSWLPAPEYTNDL
                     DVDYLVRIIMTDTGYSSRITNSKKKVNKSFQYRIEENTITYWIGENDLESKDQFFQQH
                     QLEPMLARAIRNHWCTLTIPQKPGTM"
     gene            1137..2324
                     /locus_tag="PLQ30_07725"
     CDS             1137..2324
                     /locus_tag="PLQ30_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02375.1"
                     /translation="MKLREAKLPLEVIILIIAGILVLTTGVLLFSVYKGALPYFENGL
                     YGLLLIIFALQTITLGRTPFGDLPRSTLVLFLGLLIAVIGMVTCLIPISIRLPRILLF
                     LCFGPGGLLLFLQLLFAKDKLRRWAHYGGIFRHLIVACGAVYVLSMVIALLLLNQRLL
                     TTPLTAVVILVFGVAVFYLAIVLEKIDRTYPEAAKSAEGNVKLSTEQVMILFTGIFMV
                     IIGIVLIPVNLGILPFSASAQLGILMVIFAIQMLAFGNTPIGPFPRSWIVLAFGLLFA
                     LLGAISCIIPEILVQFLTILVGVLNILGGVLALAKMLPSKQRRPEEPRKPTPPILTKL
                     FATQLTLNLLQILFGMSMLVPGMVPGLVIGVILAANGCVLLYLLHILLMLDTMRSEME
                     AAK"
     gene            2351..3136
                     /locus_tag="PLQ30_07730"
     CDS             2351..3136
                     /locus_tag="PLQ30_07730"
                     /inference="COORDINATES: protein motif:HMM:NF018066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HPW02376.1"
                     /translation="MFKFEEGKCYKMPAHFGGVPFDPDAKAIYDDVVTIDFCYTTDAE
                     KLSNYLPEGLELIKPELTVEYQQCRQVEWMAGSAYNLVCVTVPVRFNGRHDHLEGIFV
                     LVMWENKTTPILTGNMMGVPKIYADIEDLHILADNFRTCMSFEGKTFLELEAIRQTAM
                     SGDQVKAMDTDLNTIGWRYIPKVGAPGADLSQPILFPMHRTPKVGWTGTGTLKWTALT
                     WAQNPLQYYIIDQVAELPILDIAPIVITQGEQVLTEARGRVLS"
     gene            3230..4045
                     /locus_tag="PLQ30_07735"
     CDS             3230..4045
                     /locus_tag="PLQ30_07735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW02377.1"
                     /translation="MEEYTKYYENKVAVVTGAASGIGLALCELLLEYGARTIVMADLN
                     GEKLKAESQRLELAYPKKVLAIQTDVSQKESVEAMIAEAARFGGGQIHFLFNNAGLGL
                     WKRFDDTTDKDWNFAFDVNFFSAFHGIRAVLPIMRAQGGGHIANTASGAVYSPMAEQS
                     MYCATKSALLGLTGALRYELWDENIRFSTVIPGSVATSIWDNVGGPPASAISPRESAK
                     GILKGLAANERVVIVTEADRDAARNTYRPEIAQHTDEYFLGVARQRREGKLGL"
BASE COUNT         1153 a          987 c          993 g         1151 t
ORIGIN      
        1 cccaactccc cttaagagaa aagcggagta tgtacctgct tatgcttatg catgagatac
       61 ttcctcccaa atctcttaga aaaatacaca aggttcgtaa cagtattcac gcattgaatc
      121 taacggacct tggtcagcat cgccttgcat caaactagcg aacaagggat tcatgagaat
      181 ctgatagaac acatcggcgc tatggtcgct attgatcgag attctgtact cgacatcgac
      241 acagctcgac aacaatagaa taagcagcac gcatcctaca gcgattccaa attttttgct
      301 aacgcgcata gaacctcctt atggtctgcg tgagattgct aaatattgac ccacaaagca
      361 tatccagtcg ttattagcgc catactattc ctatcatatt gtgatataat atctaaatat
      421 aatcaaagga agaggactat atgaagaagc tcttgattgt agttttttct atggtttctt
      481 ctctgtgcct tttcccccaa cctatggaga attcagtcaa ttatgtttct atactgctaa
      541 aaaatgaagc ataccttggc ataaaaagca ttgattggag atatgaaaat aatcaatatg
      601 tgttcacctt ggccctcaac aacgctcttc ttttcgaaaa actcaccaat aaaaatacat
      661 tcatagaatg ggacattttt atcgatattg attcgaatac caaaacatat tcgtggttgc
      721 cggcacctga atatacgaat gatttagatg ttgattattt ggtcagaata ataatgactg
      781 atactggata tagttctcga ataacaaact cgaagaaaaa agtcaacaaa tcatttcagt
      841 atagaattga ggagaatact attacttatt ggatcgggga aaatgatctt gaaagtaaag
      901 atcaattttt tcagcagcat cagttggagc ctatgttggc aagggcaatt cgcaatcatt
      961 ggtgtacttt gacaattccc cagaaaccag gcactatgta gtaagtagac aatagaaaaa
     1021 gtattggagt ccaacagtcg cattgactcg atattctttc ttattgtttc tgatatcgag
     1081 atcctcggca atatcgttgc tgctatcagc gctgttatca gcgaaggacg gaatatatga
     1141 aattgaggga agcaaagctt cctctcgagg tgataatcct cattatcgct gggattttgg
     1201 tactaacgac tggggttttg cttttctccg tgtataaggg agcgctccct tacttcgaaa
     1261 acgggctcta tggtttgctt cttatcatat ttgcgctcca aacaataaca ctcggcagaa
     1321 ctccgtttgg agatctacca aggtcaacgc tcgtgctctt tcttggcttg ttgatcgcag
     1381 taattggcat ggtgacctgt ctcattccga tatcgattcg ccttcctcgc attctgttgt
     1441 ttctctgttt tggcccaggc ggcttgctgc tttttttaca gcttctgttt gccaaagaca
     1501 agctcaggcg ttgggcacac tatgggggca tttttcgaca tctcattgtt gcgtgtggcg
     1561 cggtctatgt tctgtcaatg gtcattgcgc tgctcctttt gaaccagcgc cttttgacga
     1621 cgccgctcac ggcggtggtg atcctcgttt ttggcgtggc agtcttttat cttgcgatcg
     1681 tactcgaaaa gatagatcga acatatccag aagcagcaaa aagcgcagaa ggcaatgtca
     1741 agctctccac cgagcaggtg atgatattgt tcactggtat tttcatggtg atcatcggca
     1801 ttgtgttgat tcctgtcaat ttgggcatac ttcccttttc agcaagcgca cagttaggaa
     1861 ttcttatggt aattttcgct atccagatgc ttgccttcgg aaacactcca attggcccat
     1921 ttccgcgcag ctggatagtg cttgcgtttg gtcttctttt tgccctattg ggagctatat
     1981 catgcatcat tccagaaatt ttggtacagt ttctgacaat tcttgtcggc gtcctcaata
     2041 ttttgggcgg cgtactagct ctggcaaaaa tgctgccttc caaacagcga cgcccagagg
     2101 aaccgcgcaa acccactccc ccaatactca ctaagctctt tgcaacacag cttaccctca
     2161 acctcctaca aatccttttt ggcatgtcga tgctcgttcc tggcatggtt ccagggctgg
     2221 ttatcggtgt aatccttgct gcaaatgggt gtgtgctcct ctatcttctc cacatcttac
     2281 tcatgctgga tacaatgcgc agcgagatgg aagctgccaa ataagatgta tctttgcaag
     2341 gaggtttagg atgttcaagt ttgaagaagg aaaatgctac aaaatgccag cacactttgg
     2401 cggggttcct ttcgatcccg atgccaaagc catttacgac gacgttgtta cgatcgactt
     2461 ttgctacacc acggacgccg agaagctttc caactacctg ccggaaggtc tcgagctcat
     2521 aaagcccgaa ttgaccgttg aatatcagca gtgcagacag gtcgaatgga tggccggctc
     2581 ggcctacaac ctagtctgtg ttaccgttcc ggtacggttc aatggccgtc acgaccatct
     2641 ggaagggata tttgtgctcg tcatgtggga aaacaagaca acgccgattc tgacagggaa
     2701 catgatgggc gtccccaaga tttatgccga cattgaagat ttacatattc ttgccgacaa
     2761 cttccgaact tgcatgagtt ttgagggcaa aacgttccta gagttggaag cgattaggca
     2821 gacagccatg agcggggatc aagtaaaggc aatggacacc gacctcaata ccattgggtg
     2881 gcgctatata ccaaaagtgg gcgctccggg agcggatttg agtcaaccga ttctctttcc
     2941 aatgcaccgc accccaaagg tcggctggac tgggacagga acgttgaaat ggaccgcttt
     3001 gacctgggca caaaacccgc tgcagtacta cataatcgat caagtggcag agctgcccat
     3061 actcgacata gcgcctatcg tgataacgca aggcgagcaa gttttgacgg aagcgcgagg
     3121 gcgtgtctta agttaaagtc gctctcggca gtatgtccgc agcaacaaat agtctaataa
     3181 gaaatggtat ccatcggtga atagtggtat ccaaaggagg tcataaatta tggaagaata
     3241 cacgaaatac tacgaaaaca aagttgccgt tgtcaccggt gccgcttcag gcataggcct
     3301 cgcgctttgt gagttgctct tggagtatgg tgcaagaacc atcgtcatgg cggaccttaa
     3361 cggcgagaaa ttgaaggcgg aaagccaacg actagagctt gcatacccca aaaaagtgct
     3421 tgcaatccag acggatgtct cccaaaaaga aagtgtggaa gcgatgatcg cggaagcagc
     3481 tagattcgga ggaggccaga ttcattttct tttcaacaac gcaggccttg gcttgtggaa
     3541 gcgttttgac gacacaactg acaaggattg gaattttgcc ttcgatgtaa atttcttcag
     3601 cgcgttccat ggcattcggg cggttctgcc catcatgcgg gcgcagggag gcggccatat
     3661 tgcaaacacg gcttccggtg ccgtttactc gccaatggcg gagcaaagta tgtactgtgc
     3721 cacaaaatcc gctttgcttg ggcttaccgg cgcgctgcgt tatgaactct gggacgagaa
     3781 tatccgcttt tcaaccgtta ttccaggctc cgttgccacc tcaatttggg ataatgtcgg
     3841 aggccctccc gcttccgcca tttcgcctag agaatcggca aaaggcattc taaaaggact
     3901 ggcagccaat gaaagggtcg ttatcgtcac ggaagccgat cgcgacgccg ccaggaatac
     3961 gtaccgcccc gagattgccc aacatacgga tgaatatttc ctaggagtcg ctcgacaaag
     4021 acgagaagga aagctaggcc tctgaataag ctatggcgct ttctcccgtg gacctaaaga
     4081 ccgcgaagac ataacgaata gagaggctac attcccattc cagctccatg atttataatg
     4141 taggtgtcca gtagagaaac acaagcgata gcagaaatgc aagcgagata aaactaacaa
     4201 tgatacctgt caggacgact tttggcagaa tgatatattt ttaggctgtc taatagcatt
     4261 gtcaagtttg cgctgcttag ctag
//
LOCUS       DAOMZL010000120         4128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_20433_length_4128_cov_13.839921, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000120 DAOMZL010000000
VERSION     DAOMZL010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20433_length_4128_cov_13.839921"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="PLQ30_07740"
     CDS             complement(<1..1443)
                     /locus_tag="PLQ30_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02378.1"
                     /translation="MASHDSRFFDSENKKDVFPFADNDNPLDLVIAYIGGGSRGWAWT
                     LMSDLALEGSLRGIVRLYDVDKKAAETNAQIGNALRERDDVLGKWRYEIAEDLESALR
                     DADFVIISILPGTFAEMASDVHSPEEYGIYQSVGDTTGPGGILRALRTIPMYEVIGRA
                     ICEFCPKAWVVNYTNPMALCLRALYEIFPDIKAFGCCHEVFGTRKLLAAMVRERLGSA
                     GIPDSRDATMQQIEVNVLGVNHFTWIDRASFGTVDLIPHYRAFVDEHFELGYEGAEGE
                     SWLNSYFASAERVKFDLFRRYGLIAAAGDRHLAEFMPGRMYLRDPNTVRSWKFSLTPV
                     SWRIENAERLRQKAVNYAEGRDRFELKHSGEEGVKLIKALCGLGSLVTNANLPNHGQI
                     KGLPLGAVVETNALFQRDCVQPVDAGRLPAQIEALVAPHAITQDIVLDAALRRNAWAA
                     FSAFLHDPLMTATPLEAEALFSSMLRATANF"
     gene            complement(1569..2558)
                     /locus_tag="PLQ30_07745"
     CDS             complement(1569..2558)
                     /locus_tag="PLQ30_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPW02379.1"
                     /translation="MVTIRDVAEHAGVSVSTVSHAISGKRPISQVTRQRIFQAIEQLG
                     YEPNPAARALRTKSTGVIGFFAFDITEVFAARIIHGVERIVREKNCFLLFTSGVGFNY
                     DLAQGIDFLRKRRVDGIIIAYGVRQEISPKTFDLSNIPLVTINTFVDLTIPSLHPDDF
                     QGGKEAALHLVSKQARHPAMIAGPENRLASKERLLGFQKGLEECGMPFDASERVMYGD
                     FGAESGSYCFDALLKACPDLDAVFCANDYMAAGAINRAITSGISIPQDMHIIGYDDRE
                     FAAFWPIPITTFAQPLEYMGELGATMLFERIDGHNPEPSMIKLAPTLIARQSS"
     gene            complement(2576..>4128)
                     /locus_tag="PLQ30_07750"
     CDS             complement(2576..>4128)
                     /locus_tag="PLQ30_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HPW02380.1"
                     /translation="QEDIPCEGMYFSSGYAKAENGERYTFVWNEKKFPHAEDFIQNLR
                     NRGYRICCNVKPGILLDHPWYDELAKDGVFIPDNDGSPLRSYYWGNSASFVDFSRKEG
                     FDWWVKSLKKYILEKGISGVWNDNNEFEIEDKSLPIQAVKNILPIKMAEASYSALRSL
                     YPGKRPWLISRSGYAGLQRYARTWTGDNVSSYDTFRFNIAMGMNLGLSGLPFYGHDIG
                     GFVGPTPNEELLLRWCQSAVFQPRFVMHSWKPDGSITEPWLYPNRLSDIRNFIRMRYR
                     FLPYIYDLAIRASETGIPMESPVALEFPEDISLSLDSLDRMAGDAILVPAPPEQGKDE
                     VSVLLPKGINWFDPIHQIIYGGGEKLSFDYYPHDIQYFFRCGTVIPTFHKIEAIGKSL
                     LNSYEFIVIPPAEKSDIGLLPIACIHSEDDGESDFIPGSHWRWMMEYTVEGENDYCLH
                     IVQTEGVADKYDSSVFGKEWRFCVPDGFMLYDENEMKMGTSLVFDLDKAPEHLSLRVK
                     GAYRNKHR"
BASE COUNT         1159 a         1008 c          843 g         1118 t
ORIGIN      
        1 aaaattggct gtcgctctta gcatgctaga aaaaagagcc tcggcttcta aaggcgtagc
       61 tgtcatcaga ggatcatgca agaatgcaga aaatgcggcc caagcgttgc gtcgcagagc
      121 agcgtccaag actatatctt gagtaatggc atgaggcgca accagagctt cgatttgagc
      181 aggaagtctt cctgcatcta caggctgaac gcaatcgcgc tgaaaaagag cgttggtctc
      241 caccactgct cccaatggaa gcccttttat ctggccgtga tttggcaaat tggcattcgt
      301 aacaagcgaa cctaatccgc agagagcttt tatgagcttt acgccttctt ccccggaatg
      361 cttcagttca aatctatctc tgccttcagc atagttcact gctttttgcc tgagcctctc
      421 ggcattttct atacgccatg atacaggtgt caaagagaat ttccacgatc gaacggtgtt
      481 tggatctcgc aggtacatcc ttcctggcat gaattcggct agatgcctat cgccagccgc
      541 tgctatcaga ccataacgcc gaaatagatc gaatttgaca cgctctgcgg aagcaaaata
      601 ggaattgagc cagctttcgc cctctgcacc ttcgtatcct aattcgaagt gttcatctac
      661 aaatgctcta tagtgaggta ttaaatcaac tgtgccaaat gatgcacgat ctatccaggt
      721 aaaatgattt actccaagca cattgacttc gatttgctgc atagtggcat cccttgaatc
      781 cggtataccg gcggacccaa ggcgttcgcg aaccatcgct gctagcagct tgcgcgtccc
      841 aaatacttca tgacaacatc caaaggcctt gatatccgga aagatttcgt aaagtgcgcg
      901 aaggcaaagt gccattggat tcgtgtaatt tacaacccat gctttaggac agaattcgca
      961 tatcgctctt ccgatcactt catacattgg gatagtccgg agcgctcgca aaattccacc
     1021 tggtcccgtt gtatcgccca cagattgata gattccatat tcttcgggcg aatgaacatc
     1081 tgaagccatt tctgcaaagg tacccggaag aatagaaatg attacaaaat cagcatcgcg
     1141 cagggcgctc tcgagatctt ctgctatttc gtatcgccat tttccaagca catcatctct
     1201 ctctcgaaga gcatttccaa tttgcgcatt cgtttcagct gcctttttat caacatcata
     1261 gagccgaaca attcctctaa gcgatccttc caaggcaaga tcactcatta gtgtccaagc
     1321 ccatccccgt gagcctccac cgatataagc tatgacgaga tctaatggat tatcattgtc
     1381 tgcaaaagga aagacatctt tcttattctc agaatcaaaa aagcgagaat catgactggc
     1441 cattcattat tccttgcata gaacttaaat gtaagtgccc aacaatacta catttgctct
     1501 taaatgaaaa ggacgagcaa aaaaatcgtg gataaagata aggctcgcac caccaatgca
     1561 atctaatcct atgacgattg acgagctatg agggttggcg ccagtttgat catgcttggc
     1621 tcaggattgt gcccatcgat gcgttcaaaa agcatagttg caccgagttc tcccatgtat
     1681 tcgaggggct gcgcaaatgt agttattgga ataggccaaa aagcggcaaa ttcacgatca
     1741 tcatatccaa tgatatgcat atcctgggga atcgagattc cagaagttat ggctcggttt
     1801 attgctccag cggccatata atcgtttgca cagaagaccg cgtccagatc gggacaggct
     1861 ttcaataagg catcaaagca ataagaaccg gattcagcgc caaaatcccc atacatcacc
     1921 ctttccgatg catcaaaagg cataccgcat tcttccagac ctttttgaaa acccaaaagg
     1981 cgttctttgc ttgcaagcct gttttcgggg cctgctatca tagcaggatg ccgtgcttgc
     2041 ttagaaacca gatgcaacgc agcctctttt ccaccctgga aatcgtctgg atggagagat
     2101 ggaatagtaa gatctacgaa agtgtttatt gtcaccaaag gtatattgga aagatcgaaa
     2161 gttttcgggc ttatttcctg acgcacacca taagctatga tgatgccatc gacccttcgc
     2221 ttgcgtaaga aatctatacc ctgagctaaa tcataattaa agccgacacc acttgtaaaa
     2281 agcaaaaaac aattcttctc acgaactatt ctctcaacac catgaattat tcttgcggca
     2341 aagacttctg taatgtcaaa agcaaaaaaa ccgataaccc ccgtgctctt ggttcgaaga
     2401 gcacgtgcgg caggattggg ctcataaccg agttgctcaa ttgcttgaaa tattctctgt
     2461 ctcgtaacct gacttatagg acgtttgcca ctaattgcgt ggctcaccgt cgagacagaa
     2521 actcctgcat gctctgctac atcgcgaata gttaccattc atctagaata tcatcttatc
     2581 tatgtttatt gcgataggct cccttgactc taagagaaag gtgctctggg gctttgtcaa
     2641 gatcaaatac aaggctcgta cccattttca tttcattctc gtcatagagc ataaaaccat
     2701 cgggtacgca aaatcgccat tctttcccaa agactgagga atcatattta tcagcaacgc
     2761 cttcggtctg cactatgtgg agacaataat cattctctcc ttccactgta tattccatca
     2821 tccatcgcca atggcttcca ggaataaaat cactttctcc atcgtcttcg ctatggatac
     2881 aagcaatagg tagtaggccg atatcgcttt tttctgctgg aggaataaca atgaattcat
     2941 aactatttaa aagagatttg ccaattgctt ctattttatg aaacgtgggg attaccgtac
     3001 cgcatctgaa gaagtattga atgtcatgag ggtaataatc gaagctcaat ttctctccgc
     3061 ccccatatat aatttgatga atcggatcaa accaatttat ccccttagga agcagcaccg
     3121 atacttcgtc ttttccttgt tcaggcgggg ctggtacaag aatagcatcc ccggccatcc
     3181 tatccaggct atctaatgaa agcgaaatat cctctggaaa ctcaagcgca acagggcttt
     3241 ccattggaat gccagtttca cttgcgcgaa ttgctaaatc atagatatat ggcaaaaatc
     3301 ggtatcgcat tcgtataaaa ttgcggatat ccgaaagccg gtttggatat agccatggtt
     3361 cggttatgct cccatcaggt ttccatgagt gcataacaaa gcgtggctga aagacagcac
     3421 tctggcacca acgcagcaat agctcctcgt tcggcgttgg gccaacaaat ccaccaatgt
     3481 catggccata gaatggtagg ccactgagcc ctagattcat ccccattgca atattgaagc
     3541 ggaatgtatc atagctggac acattatctc cggtccatgt gcgggcatag cgctgaagcc
     3601 cggcatatcc ggagcgactg atcagccaag gccttttgcc aggatataag gaccttaatg
     3661 cgctataaga tgcttccgcc atctttattg gcaaaatatt cttgacagcc tgaatcggaa
     3721 gagacttatc ctcgatttcg aattcgttat tgtcattcca cactcccgaa attccctttt
     3781 cgaggatata tttctttagc gatttgaccc accaatcaaa accttctttt cgggaaaaat
     3841 cgacgaatga cgcgctgttg ccccaataat agcttcttag gggagagcca tcattgtcag
     3901 gtataaaaac cccatccttt gcaagctcat cataccatgg atgatccaag agtatgcctg
     3961 gctttacatt acagcaaatt cgatatcctc tgtttctgag attctgaatg aaatcttccg
     4021 catgggggaa ttttttttca ttccatacaa aagtatagcg ctcgccattt tcggcttttg
     4081 catagcctga agaaaaatac atgccttcgc agggtatatc ttcttgtt
//
LOCUS       DAOMZL010000121         4018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_21238_length_4018_cov_10.956599, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000121 DAOMZL010000000
VERSION     DAOMZL010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21238_length_4018_cov_10.956599"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PLQ30_07755"
     CDS             <1..407
                     /locus_tag="PLQ30_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPW02381.1"
                     /translation="FQLNRKVTDDDIIEIYKGLWRIEETFKVTKSELKARPVFLSTEN
                     HIRAHFLICFVTLLLMRLLEYRLGWKYSSAAIQESLASACGTRIDEKLYVFDYYDAVL
                     EAIGKDLGIDFSRQSLTAQEIRHLLAHTKQRT"
     gene            complement(549..1148)
                     /locus_tag="PLQ30_07760"
     CDS             complement(549..1148)
                     /locus_tag="PLQ30_07760"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HPW02382.1"
                     /translation="MIDGTIDRVIDREIDRTALQFIAIDMDGNILDDSYRLSDRVVAV
                     LATLHTQGKKIIIATGRIFMAAQHYLIEKIEPDRYVCTNCADIFEPKGVQIAAYHIPP
                     QAVPVLIELGRAHEVVAHEVLMCCYISDQWFYEKPLPAVEFYQKRTGIQGLQRNIESF
                     EGEDILKFLAIGPHEEILAIRDELGRKAPTMLEIIANSD"
     gene            1293..2588
                     /locus_tag="PLQ30_07765"
     CDS             1293..2588
                     /locus_tag="PLQ30_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPW02383.1"
                     /translation="MKRPMLIVALEMVFAIALVGVVSAQTINVLFWDDAYPRTLMEKI
                     PEFEKATGIKVNIEILQPPQVFTKTSVSVTKERTDYDLVCVDEGNIPLFASLMLPYDQ
                     WGPGKIYKKIDPNTVTPAMLEVGQWDGKLVGLPINGNLYVWMTRKDLIENPKYKADFK
                     AKYGYDLGVPQTFQQMLDMGTYFYDNGIVSGGFGPFNGGPAGVFGEAIFMWYSFGTHF
                     IDWINGKPTLVVDKAKAVEGMEFYKKLMKIAPKGAETMAHVERQAAFCTDPKGVFTQF
                     NWPAQIASYEDPDKSLVSGKIVYSAPPAGPSGRFSIRGTWAVCIPLATKNKDAAAEFV
                     YWWASKEIASWLVEKNTVPARTDALTDPQFAKTKPWLPAIAESMKYAVARPRFNEVAE
                     VQDIVKKYWIMGITGQMPCNDAMQKIIDETKNVLKKAGY"
     gene            2563..3522
                     /locus_tag="PLQ30_07770"
     CDS             2563..3522
                     /locus_tag="PLQ30_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPW02384.1"
                     /translation="MSSKKLDIKQKNATSKTNKRTKEGKARGRAPFLFLAPAIIIVFA
                     VLIFPILFSLVVSTYNWPLSEGAGIRRFVGFGNYLALTQDSEFWNSLKLQLGFIFIAI
                     PIELLFGFAAALLLNREFFGARIVRSLLLLPVFFLPILSGMTWRFMLQPRYGPLNNLL
                     IKLGAPEISWLGNPTTAYIAIIVQDVWRMWPFMFMLLYAGLTAIPQELLEAAEIDGAG
                     FWKKLWSVIIPLLMPTILTSVLLRIIDALRVFSEVYVMTEGGPGSATMLFSLYTQRQA
                     FGYQKVGMASAMAIFLLIISIVFALTLVRKNMSLDVLEEKAGA"
     gene            3522..>4018
                     /locus_tag="PLQ30_07775"
     CDS             3522..>4018
                     /locus_tag="PLQ30_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02385.1"
                     /translation="MDRRSRRRKRTRKFLINLVVAVVAFVILAIELYPIAITVLNGFR
                     RDIHILSGQPFKLSQLTLRSYQLVLKNAGFRIGMRNSIVVGLLSTFISLLVGAMASYG
                     IARFKFKGRNSLAYSFLVLRMLPQVSLVIALYLMFSDIGLRDTISGLTLAHTSFNVPY
                     VIWLL"
BASE COUNT         1011 a          916 c          977 g         1114 t
ORIGIN      
        1 tctttcagct caatagaaaa gtgactgacg atgacatcat agagatctat aagggcttat
       61 ggcgtattga ggagaccttc aaggtaacaa agagtgaact taaggctcgg cctgtctttc
      121 tctctaccga aaaccatatc cgtgcacatt tcctcatttg ttttgtcacc ttactcctca
      181 tgcgcctcct cgagtatcgc cttgggtgga agtattcctc agctgcgatt caggagtcct
      241 tagctagtgc ctgtggcaca aggatcgatg agaagctcta tgtgtttgac tactatgacg
      301 cagtccttga ggcgattgga aaggatcttg gtattgattt ttctcgccag tcgctcaccg
      361 ctcaggagat taggcatcta cttgcccata ctaagcaaag aacctaggag atgtactaca
      421 atctatatca agaagatatg ctcactagtc attatagaat aatagattat aacattattt
      481 tagaacgcca actgtcaaag tcaggtttta agtccctggt gtctacctat tctaccaccc
      541 cgacttgctt aatcgctatt tgctatgatt tcgagcatcg taggtgcttt gcgtccaagt
      601 tcatcgcgta ttgcgagaat ctcttcatgt ggcccaatag ccaaaaattt tagaatatcc
      661 tcgccttcaa aactctcaat attccgttgc agtccttgga tgcccgttcg cttttggtaa
      721 aattcaactg ctggcaaggg tttttcatag aaccactggt cgctgatgta acagcacata
      781 agaacctcgt gtgcgacaac ttcgtgtgcg cgtccgagct cgatgaggac cggtactgct
      841 tgcgggggaa tatggtacgc agcgatttga actccctttg gctcaaagat atcagcgcaa
      901 ttcgtgcaga catacctatc gggctcgatt ttttcaataa gatagtgctg ggctgccata
      961 aatatacgcc ctgttgcaat gatgatcttt ttgccttgtg tgtgcaatgt tgcgagcacc
     1021 gcgacaacgc ggtcagagag gcggtagcta tcatcgagaa tgtttccgtc catgtctatc
     1081 gcgatgaatt gaagcgcggt gcgatcgatt tcacgatcaa ttacgcgatc gattgtacca
     1141 tcaatcatac cattgattgt gcggggaata gtaatgtttt gatgctacag aaaaaatgga
     1201 ttttagtcag cagaaacgaa aatatttctt gacaaccgcc atactccatg atttactttc
     1261 attgggaaaa taaaacaccc aaggaggcca ttatgaaaag accgatgctg atagtcgcgc
     1321 ttgaaatggt gtttgcaata gcgctcgtgg gcgtagtgtc cgcccagact atcaatgtgc
     1381 tgttctggga tgatgcctat ccacgcacac tcatggaaaa aatccctgaa tttgagaagg
     1441 caacaggaat caaagtgaat atcgagattt tgcagccacc gcaggtattc acaaagactt
     1501 cggtgagcgt aacaaaagag cgtactgact acgatcttgt ctgtgtcgat gagggtaata
     1561 ttcctctttt tgcctcgctc atgttgccct acgatcaatg gggccctgga aaaatctaca
     1621 agaaaatcga cccgaacacc gtgacgccag ccatgctcga ggttggacag tgggatggca
     1681 agctcgtggg cctgcctatc aatggcaacc tctatgtgtg gatgacgcgc aaggacctca
     1741 ttgagaatcc gaagtataag gcagatttta aggctaaata cggctatgac cttggtgtgc
     1801 cacagacatt ccagcagatg ctcgatatgg ggacttattt ctacgacaat ggtattgtct
     1861 caggcgggtt tgggccattt aatggcgggc cggcaggcgt ctttggtgaa gcgatcttca
     1921 tgtggtattc gttcggtacc catttcatcg attggatcaa tggcaagccg acgctggttg
     1981 tcgataaggc aaaggctgta gaaggcatgg agttctataa gaagctcatg aaaattgcgc
     2041 caaaaggcgc tgagaccatg gcacatgtcg agcgacaggc agcgttctgc actgatccaa
     2101 aaggcgtatt cacccagttt aactggccag cgcagattgc aagctatgaa gaccccgata
     2161 aatcgctcgt ttctggcaag attgtctata gcgccccgcc agcgggtcct agtggcagat
     2221 tctccatacg aggaacctgg gcggtctgca ttccactagc cacaaagaat aaggatgctg
     2281 cagcagaatt tgtttattgg tgggcttcca aggaaatcgc gagttggctt gttgagaaaa
     2341 acacggtgcc ggcgcgcacc gatgcgctca ctgatccgca gttcgcaaag acaaaacctt
     2401 ggcttcccgc aatcgcagaa tcgatgaagt acgcagttgc acggccgcga ttcaatgaag
     2461 tggctgaagt tcaagatatt gtcaagaaat actggattat gggaatcaca ggacaaatgc
     2521 catgcaatga tgcaatgcag aaaataatcg atgagacgaa aaatgtcctc aaaaaagctg
     2581 gatattaaac agaaaaacgc aacaagtaaa acaaataagc ggacaaaaga aggaaaggcg
     2641 cgcgggcgcg cgcctttcct gtttcttgct cctgcaatta tcattgtttt tgcggttttg
     2701 atatttccta tactattttc tcttgtggta tcgacttaca attggccgct ctccgaagga
     2761 gcaggaatac ggcgctttgt cggctttggc aattaccttg cgctgactca agactcagag
     2821 ttttggaata gccttaagct gcagctcgga tttattttta tcgcaattcc gattgagtta
     2881 ctctttgggt tcgccgctgc gctactcttg aatcgcgaat ttttcggcgc ccgcattgtg
     2941 cgaagtcttt tgcttttgcc agtgtttttt cttccaattc tgtctggtat gacatggcga
     3001 tttatgctgc agcctcgcta tggcccgctc aacaatctcc tcattaagct tggagcgcct
     3061 gagatttcgt ggcttggcaa tcccacaact gcttatatcg caattatcgt gcaagatgtt
     3121 tggcgcatgt ggccctttat gttcatgctc ctctatgcag ggctgaccgc gattccccaa
     3181 gaactgttag aggctgcaga aatagatggt gctggttttt ggaaaaagct ttggtcggtt
     3241 atcattcctc ttttaatgcc cacaattctt acctcggtgc tgctgcgtat catcgacgcg
     3301 ctgagagtct tcagcgaagt ctatgtgatg accgaaggag ggccaggctc tgctaccatg
     3361 ctcttttcgc tctacacgca gcgccaagca tttggctatc agaaagtggg tatggccagt
     3421 gcaatggcaa tattcctgct cattatttcg atcgtattcg cgcttaccct cgtgcgcaaa
     3481 aatatgtcgc tcgatgtgct cgaagaaaag gcaggtgcat gatggatcgc agaagtcgaa
     3541 gacgaaaaag aacgcgaaag ttccttataa acctcgttgt tgcggtggtg gcctttgtga
     3601 ttcttgctat agagctctat ccgatcgcaa ttacggtgct taacgggttt cgacgcgata
     3661 tccacattct ctcaggccag ccattcaagt taagccagct gacgttgcgc agctatcagc
     3721 ttgtgcttaa aaatgcgggc tttaggattg gaatgcgaaa ttcgatcgtc gtggggcttc
     3781 tgtccacctt tatttcattg ctagtgggtg caatggcatc gtatggtatt gcaagattca
     3841 aattcaaagg acgaaattca ctcgcttata gcttccttgt gttgcgcatg ctgccgcagg
     3901 tttccttggt tatagcgctt tatctgatgt tctcagatat tggtttgcgg gacacgatca
     3961 gcggactcac cttggcgcac acgagcttta atgtgccgta tgtcatttgg ctgctttt
//
LOCUS       DAOMZL010000122         3977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_21536_length_3977_cov_16.624681, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000122 DAOMZL010000000
VERSION     DAOMZL010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21536_length_3977_cov_16.624681"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PLQ30_07780"
     CDS             complement(<1..450)
                     /locus_tag="PLQ30_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HPW02386.1"
                     /translation="MMRNKQFIAIFVVIAMASIAVGTVSAQTSTQSNRLNNELIIWTY
                     DSFISEWGPAPLIAQAFQKEAGIKLRFVSKGDGGALLSALFDPINKPSADIIIGLDDK
                     QLEKAIKSGLFAPLNLKNLSEIQKDLLLDKTNRLVPFDFSQFALIWDS"
     regulatory      complement(437..547)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(551..1804)
                     /gene="clpX"
                     /locus_tag="PLQ30_07785"
     CDS             complement(551..1804)
                     /gene="clpX"
                     /locus_tag="PLQ30_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPW02387.1"
                     /translation="MARNKQFDLEQETFCSFCGKSSSYVRKMITGPGVNICDECVRIC
                     SQILDEEEQRVTASFLTEVPKPKEIKEFLDMYVIGQEYAKRVLSVAVYNHYKRLVHKV
                     SAQSPDVEIEKSNVLLIGPTGSGKTLLARTLARKLKVPFAMADATTLTEAGYVGEDVE
                     NILLKLIQNAGGNIQAAERGIVYIDEIDKISRKGENPSITRDVSGEGVQQALLKIIEG
                     TISNVPPQGGRKHPNQEYLRIDTTNILFICGGAFVGLDKIIESRISSHPMGFGADVKA
                     RHEKMLSQLYSELQPDDLIKFGLIPEFVGRLAVKVALDELTKEDLKRIIIEPKNALLK
                     QYKASMQLDNVELEFDDAAIDAIAEKAIVQKTGARGLRAIIEKIMVDIMYSLPSDNNI
                     KKVILTRDAVEGKGSCIFEYRQKTA"
     gene            complement(1791..2402)
                     /gene="clpP"
                     /locus_tag="PLQ30_07790"
     CDS             complement(1791..2402)
                     /gene="clpP"
                     /locus_tag="PLQ30_07790"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPW02388.1"
                     /translation="MSEYMSNLVPIVIEQTGIGERSYDIYSRLLKDRIVFIDGEISDV
                     IADLVIAQLLFLESQDPSRDISIYINSPGGLVTAGLAIYDTIQYIKPDVQTICLGQAS
                     SMAALLLACGTKGKRYALPSSRILIHQPWGGVSGQASDISLQAREIIRLKKLTIDYFA
                     LHSGQPIEKVAQDMERDFYMSSQEAVEYGIVDTIMEPRKHGKE"
     gene            complement(2469..3824)
                     /gene="tig"
                     /locus_tag="PLQ30_07795"
     CDS             complement(2469..3824)
                     /gene="tig"
                     /locus_tag="PLQ30_07795"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPW02389.1"
                     /translation="MEIAEKRIEELELSRIRMTITVRAQDLRSEYDAMINEYAKQARI
                     DGFRKGHVPISVLERKFGPSLKTDAKARVVEKALETALKDVSRPPLAYEMPELENDPS
                     FELDSDFTFSVSYDAFPVFELPSLDGIEIEIPAVAITQEDIDRELEDIRQRNAIVVEK
                     DGPSEIGDIVIASFVELAEDGSEISGTSRQDFSFELGKNLNIYKFDDEIVGLKAGDEK
                     TIVKTFPDDFEYPEYAGKTVTIKVSVSKVKHQDLPDLDDELAQDVSEKYKTLDDLKEA
                     VRAHLTENLQTRLRKAKEHAIMDELLKKTKISIPRSMLATEIAMRWESLKQQMGVDSE
                     EHLEMLLEMSGKTREQIYNEWGPLAEKALATNILLDKLIEAGSYTSTDEEVDAEIAKE
                     ASHSTMSPAEIKTEYEKHGTIKYIKEDIAIRKFFDDILSSTTVKESGQMSYLDFMKKN
                     D"
BASE COUNT          990 a          922 c          839 g         1226 t
ORIGIN      
        1 agagtcccag attagcgcaa attggctaaa atcgaaaggt acaagcctat ttgttttatc
       61 cagcaataaa tccttctgga tttccgagag gttttttaga ttgagcggag caaagaggcc
      121 actttttatg gctttctcga gttgtttgtc gtcgagtcct attattatat cggcgctagg
      181 cttgtttatg gggtcgaata gcgcagacag tagcgctcca ccgtctccct tggacacaaa
      241 gcgaagcttt attccagctt ctttctggaa agcttgagca ataagaggag ccggtcccca
      301 ctctgaaatg aatgaatcat aggtccagat tatcaactca ttattcagcc gatttgactg
      361 agtacttgtt tgggctgata ctgtcccgac agcaattgaa gccattgcta tcactacgaa
      421 aattgcgatg aattgtttgt tacgcatcat cttatccctc cgccggcatt atccggatca
      481 ggttcgatgg gtgtttctca ggctgctttc tccaacggag aaaagcaaac caccccattg
      541 atttctgcgc ttaggctgtt ttttgacgat attcgaagat gcagctgcct ttgccttcta
      601 ccgcatctcg cgtcaggatt acctttttta tattattatc acttggaagc gagtacatta
      661 tatccaccat gattttttca ataatagcgc gtaagccgcg cgctcctgtc ttctgaacaa
      721 tagccttttc agcaatagca tctattgcag catcatcaaa ttccagttca acattatcga
      781 gctgcataga ggctttgtat tgcttgagta aggcattctt cggttcgatg atgatacgct
      841 tgaggtcttc ttttgtaagt tcatccagtg caacttttac cgccaggcgt ccgacaaact
      901 cagggataag gccgaatttg ataagatcat ccggttgcaa ctctgaatag agctgactta
      961 gcatcttttc atgtcttgct tttacatccg caccaaaccc catggggtga ctggaaattc
     1021 tcgattcgat tatcttgtct agcccaacaa aggctccgcc acagatgaag aggatattgg
     1081 ttgtatcgat acgaagatac tcctgatttg gatgttttcg tcctccttgc ggaggtacgt
     1141 tagatatggt tccttcgatg attttaagaa gagcttgctg gactccttct ccagatacat
     1201 cgcgcgtaat cgaaggattc tcgcctttgc gtgaaatctt atctatctcg tctatgtaca
     1261 cgatgccgcg ttctgctgcc tgaatatttc cacctgcatt ctgaataagc ttgaggagaa
     1321 tattctcgac atcttcgccg acatatccag cttccgtaag agtcgttgca tcagccatag
     1381 cgaaaggtac tttgagtttt cgggctaaag tgcgcgcaag tagcgtctta cctgatccgg
     1441 tcggccctat gagcaataca ttagactttt cgatctcgac atctggagac tgggctgata
     1501 ctttatgtac tagtcgtttg taatggttat aaacagccac ggaaaggact cgcttggcat
     1561 attcctgtcc aatcacatac atatcaagga attctttgat ctctttaggt ttgggtactt
     1621 ctgtaagaaa actagcggtg actctctgtt cttcctcatc gaggatttga ctgcaaatac
     1681 gaacacactc atcgcatata ttgacgccgg gccctgtaat catcttgcgg acataggatg
     1741 aacttttccc acagaaagaa cagaatgtct cctgctccag atcgaattgt ttattccttg
     1801 ccatgctttc gcggctccat gatcgtatcg acgatcccgt attcgacagc ttcttgagag
     1861 ctcatataga aatctcgctc catatcttgg gcaacttttt caattggctg gccagaatgg
     1921 agcgcaaagt aatcgattgt aagctttttc aatcgaatga tctcacgcgc ttgaaggctg
     1981 atatcgcttg cttgaccaga gactccgccc catggttgat gtatcaaaat acgggaagag
     2041 ggcagggcat agcgcttacc ctttgtccca caggcgagca aaagagcagc catactcgat
     2101 gcttgtccca gacaaattgt ctgcacatcg ggttttatat attgaattgt atcgtatatt
     2161 gcaagtcctg cggttaccaa tccaccaggg ctgtttatat agattgaaat atccctggag
     2221 ggatcctgag attccagaaa caaaagctgt gcaatcacca aatccgcaat gacatcactg
     2281 atttcgccat cgatgaatac gatacgatct ttgagaaggc gcgaatagat gtcataggat
     2341 cgctctccaa taccggtttg ctcgataaca atcggaacga gattgctcat atattcagac
     2401 attgccattt ctctggtacc ttttgatttg ccataattga gattcaatat tgaactccga
     2461 aataaaatct aatcattttt tttcataaag tccaaatagg acatttgacc tgattctttg
     2521 accgttgtcg aagacaagat atcatcgaag aatttcctaa tggcgatatc ttctttgata
     2581 tactttatcg tgccatgctt ttcatactct gttttgatct cggctggact catcgtggaa
     2641 tgacttgctt ccttagctat ttcagcatcg acttcttcat cggtagatgt ataagaccca
     2701 gcctctatca gcttatctag aaggatgttt gtcgcaaggg ccttctctgc cagcggtccc
     2761 cattcgttgt aaatctgttc acgagttttt ccggacattt ctagaagcat ttccagatgt
     2821 tcttcggaat cgactcccat ctgttgcttt aaggattccc atcgcatcgc gatctcggta
     2881 gcgagcattg aacgggggat ggatatcttc gtttttttca ggagttcgtc cataattgca
     2941 tgctcttttg ctttgcgaag ccttgtttgc agattctctg tcaggtgagc gcggactgct
     3001 tcctttaggt catctagggt cttgtatttt tcggaaacat cttgggctag ttcatcatca
     3061 agatctggaa ggtcttggtg tttcactttg ctaactgaga cctttatggt cacagttttt
     3121 cctgcatact ccggatactc aaaatcatcg ggaaaggttt ttacgatggt tttttcatct
     3181 ccagccttga ggccgacaat ctcatcatcg aatttataga tgttcaaatt cttgccgagc
     3241 tcgaaagaga agtcctgacg tgaagtacca gaaatctcgc ttccatcttc tgcaagttcg
     3301 acaaaactag cgatgacaat atcgccgatt tccgaaggcc catccttctc tacgacaatg
     3361 gcatttcttt gccttatgtc ttctaattcg cgatctatat cttcctgtgt gatggctacg
     3421 gctggtattt cgatctctat tccatcaagc gaaggaagct caaatactgg aaaagcatca
     3481 tagctaacgc taaaagtaaa atcagaatcc agctcgaagc ttggatcgtt ttccagctca
     3541 ggcatttcat aggcgagtgg tggtcgagag acatccttga gggcggtctc tagcgctttt
     3601 tctacgactc gcgccttagc atcggtcttg agcgatgggc caaacttccg ctccaacaca
     3661 gaaataggaa catgtccctt tctaaaccca tcgattctag cttgttttgc atattcattt
     3721 atcattgcat catattcaga tcgcaggtcc tgtgctcgta cggtaattgt catgcgaatt
     3781 ctggagagtt caagctcttc aattcttttt tcggcgattt ccatataatc ctctcagttc
     3841 ttactctata taaattggaa aatgcgcgct atatgctata tagcgtgcta taacacaaaa
     3901 aaacagggcg attcggccag tttccgaatc gccctgttgt cgaagcggga aacgggagtt
     3961 ggacccgcga catccac
//
LOCUS       DAOMZL010000123         3939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_21809_length_3939_cov_13.248970, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000123 DAOMZL010000000
VERSION     DAOMZL010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21809_length_3939_cov_13.248970"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2008)
                     /locus_tag="PLQ30_07800"
     CDS             complement(<1..2008)
                     /locus_tag="PLQ30_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HPW02390.1"
                     /translation="MDDTTGFDLTTIEDLSVDELAISQRIASELAIRLSQMQAVLSLS
                     AEGCTIPFISRYRKERTENLDEVQVRDCIQKFQSYKNLEERRLEVLKGISALGKLDAF
                     LYENIVRATTLAELEDLWAPFKKKKKTRGMIAQEKGLSPLAELMARAFLEEVIAAAPS
                     FIHRDEEHPELSVESVEDALSGARDILAERLSQDTSMRAYIKDVLLKDGELAVKGIGD
                     EEKREQSTYQMYWDYREPLTTLKHHRVLAVNRGEREGELDVSIEVSDELVEERLLERV
                     CPANAQHKEAVIDGLFRLLLPAVRREIRSDLTESAESHAIEVFSTNLRNLLMQPPLRG
                     TRVLGIDPGIRTGTKCAALDETGKFLDYFVINQETKPEQGKKDVAAAVIKHRLSVIAV
                     GNGTGSHEVQRLVAESISENKLVCRFAVVDEDGASVYSASDLAREEFPALDLTIRGAI
                     SIGRRLQDPLSELVKIDPKSIGVGLYQHDVNQKKLANQLDEVVGSVVNQVGVNLNTAS
                     YSLLRYVSGINASLARKIVKFRNASGVIRNRSLLKEIPGLGEKTFEQCAGFLKIPESD
                     NELDNTWVHPENYTLALEILPIVKSGGEPQAEKRATLKQKYEVGDITLNDIISELRKP
                     NRDPREDLPPPLMQENVLSFEDLRPGLKVKGKVKNVVDFGAFVDI"
     gene            2203..2835
                     /locus_tag="PLQ30_07805"
     CDS             2203..2835
                     /locus_tag="PLQ30_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW02391.1"
                     /translation="MAKFSNKVRTFSALEVAKLCGVVNQTAINWIRGGHLKAFSTPGG
                     QYRVYAEDLVTFLKERDMRVPEELKDELTMPVDTGLALIVDDDKDLNTILKRLLERRI
                     EDLRVAQAYDGFEAGRIIAERHPAIVILDLNLPGVDGFSLCKKIRSDESFGQPAVIAI
                     TGIGEEEAQPRMMEAGADAFFSKPLNFDALLAKMKELMETRKNIKETEDT"
     gene            2885..3475
                     /locus_tag="PLQ30_07810"
     CDS             2885..3475
                     /locus_tag="PLQ30_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HPW02392.1"
                     /translation="MKELYRSDDSHFIVAIGDITNIACDAIVNAANSSLLGGGGVDGA
                     IHSAGGPAILAECRALRNSSLPNGLPPGKAVATTAGRLAAKRVIHTVGPIWHGGTHGE
                     DEILASCYRECLALALHEKLKSIAFPAISTGIYGFPKERAARIAWKTVQDFFSANPSA
                     FLIVWFIFFSEIDARIFLDSNGLSMNTGSKPSPEKN"
     gene            3504..>3939
                     /locus_tag="PLQ30_07815"
     CDS             3504..>3939
                     /locus_tag="PLQ30_07815"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPW02393.1"
                     /translation="MKAAIFTQFGGPITITEVPDPVVAADGVVLKIDATGICRSDWHG
                     WKGHDPDIKLPHVPGHELAGTIVEVGKDIKNWKRGDRVTMPFVAGCGHCLPCLTGNPQ
                     VCDHQFQPGFTAWGSFAEFVAIRYADMNLVRLPNTMDSSTAAS"
BASE COUNT         1019 a          997 c          909 g         1014 t
ORIGIN      
        1 cgatatcgac aaaggcacca aaatcgacga cattcttcac ttttcctttt acttttaagc
       61 ctggtcgaag atcctcgaag ctgagcacat tttcctgcat aagagggggc ggaagatctt
      121 cgcgcgggtc gcggtttggt tttctaagtt cggagatgat gtcattcaag gtgatatcgc
      181 caacctcata cttctgtttc aaagtcgccc ttttttctgc ttgtggttct ccaccagact
      241 taacaatagg cagaatttct aaggcaagcg tatagttttc gggatgaacc caggtgttat
      301 cgagctcgtt atcgctttct ggtatcttga gaaacccagc gcattgttcg aatgtcttct
      361 caccaaggcc tggaatctct tttaacagag aacgattgcg gatcactcca cttgcattgc
      421 ggaacttgac gattttcctt gccaagctcg cattgatgcc cgacacatac cgcagtagcg
      481 aataacttgc ggtattcaaa ttgacaccga cctgattcac gaccgatcca acaacctcat
      541 cgagctggtt tgctagcttt ttctggttca catcgtgctg atatagccct accccgatgc
      601 tctttggatc gatttttaca agctccgaaa gtggatcttg caggcgccgc cctatagata
      661 tagccccgcg aatggttaga tcgagagctg gaaattcttc tcgcgcaaga tcgctcgcgg
      721 aatatacaga cgcaccatct tcatctacaa cggcaaaacg gcagacaagc ttgttttccg
      781 atatagattc cgctaccagc ctctgaacct catgacttcc tgtgccattt cccaccgcaa
      841 tgaccgagag cctatgcttt attaccgctg cggcgacatc tttcttgccc tgttcaggct
      901 tggtctcctg gtttattaca aagtaatcca ggaatttgcc agtttcgtcg agcgctgcac
      961 acttcgtacc tgtcctgatt cctggatcga tgcctagcac tcttgtgccg cgcaaaggag
     1021 gctgcataag aagattgcgc agattcgtgc tgaacacctc gatagcatgg ctttccgcgc
     1081 tttcggttag atctgagcga atctcccttc gcaccgccgg caagagaaga cggaaaagcc
     1141 catctatgac cgcttccttg tgctgagcat tggctgggca cacgcgctcc agcagccgct
     1201 cttcaacgag ctcatcgctc acctcgatcg acacatcgag ctcgccttct cgttcacctc
     1261 gattcaccgc aagcactcta tgatgcttga gggtcgtaag aggctctcga taatcccaat
     1321 acatctggta agtggactgc tctcgttttt cctcgtcccc gatgcccttg accgcaagct
     1381 ctccatcctt tagcagcaca tcctttatgt aagcccgcat gcttgtatcc tgcgaaagcc
     1441 gttcggcaag gatatcgcgc gctcctgaaa gtgcatcctc cacgctttca accgaaagct
     1501 cagggtgttc ttcatctcga tggataaaag aaggagcagc ggcaattacc tcctcgagga
     1561 aagcccttgc catcagctca gcaagaggac tcagcccttt ctcctgagcg atcatgccgc
     1621 gcgtcttttt ctttttcttg aatggtgccc agaggtcctc gagttcagct agagtcgtgg
     1681 cacgcacaat attctcatat agaaaagcat cgagtttacc tagggctgag atgcctttga
     1741 gtacttctag cctgcgctct tcgagatttt tgtaactttg gaacttttgg atacaatcgc
     1801 gaacctgtac ctcgtcgaga ttttcggttc gctctttacg ataacgactt ataaatggaa
     1861 tagtgcagcc ttcggccgaa aggctcagaa ccgcctgcat ctgcgaaagc cgaatagcaa
     1921 gctcactcgc tattctctgc gaaatagcga gctcatctac cgaaagatct tctattgtag
     1981 ttagatcaaa acctgttgtg tcgtccatag cgaggctaac gttacgggaa gttcaagaaa
     2041 atttcaaggt agaaatgatg gaatgacgag ttcgctacaa atctctgcga agttctatca
     2101 tagaatgata attcgctgga tttttagata tatatggtat atcctttgaa cagcattcgt
     2161 tacattgagc tgaatatacc actattaata aagaggcatc tcatggcgaa gttcagcaat
     2221 aaggttcgaa cattttcggc gctcgaagtc gcaaaactct gtggtgttgt taatcaaaca
     2281 gcaataaatt ggatacgagg cgggcatctc aaggccttta gcacaccggg aggacaatat
     2341 cgagtctatg ctgaagatct ggtaactttt cttaaagaac gagacatgcg agttccagaa
     2401 gagctcaaag atgaactcac tatgccggtg gatactgggc ttgcactgat cgtagacgac
     2461 gataaggatc tcaatacgat cctaaagcgc ctcctcgagc gaagaattga agacctgcgc
     2521 gttgcgcagg cctacgatgg ctttgaagca ggcagaatta ttgcagaacg ccatcctgca
     2581 atagtcatac tggatttgaa tcttcctggc gtcgacggct tttcgctctg taaaaaaatc
     2641 cgatccgatg aatcctttgg ccagccggcc gtgatagcta ttactggaat tggggaagaa
     2701 gaagctcaac ctcgaatgat ggaggccgga gccgatgcat tcttttctaa gccgctaaat
     2761 ttcgatgcct tattggcaaa aatgaaagaa ctaatggaaa cccgcaaaaa tataaaagaa
     2821 acggaagata cttgaatgac aatatgctaa gaaaagtcca tgtcgaaaag gataaactca
     2881 tattatgaaa gaactatata gatctgatga cagccatttc atagtcgcaa tcggagacat
     2941 aaccaacatt gcatgcgatg caattgtcaa cgcagctaac tcgagcctat taggcggagg
     3001 tggtgtcgat ggagctatcc atagcgcggg aggcccggca atcctcgccg aatgtcgcgc
     3061 gttgcgaaac agctccttac ccaatgggct gccgccaggc aaagcagtgg caactactgc
     3121 aggccgcctt gccgccaaac gtgttatcca tacagtaggc ccgatttggc acggaggtac
     3181 ccatggcgaa gacgaaatcc tcgcatcctg ctacagagaa tgccttgcgc tcgccctcca
     3241 cgaaaagcta aaatctattg cctttcccgc catctctaca ggcatctatg gattccccaa
     3301 agaacgtgcg gctagaattg cctggaaaac agtacaagac tttttctcag caaatccttc
     3361 ggctttcctt atcgtgtggt ttatcttttt ctccgagata gatgcacgca tctttcttga
     3421 ttcgaatgga ctttcgatga atacaggatc aaagcccagt ccagagaaga attaagagaa
     3481 gaagtaaaag gaaacataaa actatgaaag cagcgatatt tacacaattc ggaggcccta
     3541 tcacgatcac agaagtgccc gatccagtag tcgccgccga tggcgttgtg ctcaaaatcg
     3601 acgcgacagg tatatgccgc agcgattggc atggttggaa gggacacgat cctgatatca
     3661 aacttcccca tgtgccggga catgaactcg ccggtaccat agtcgaggtg ggcaaagaca
     3721 ttaaaaactg gaagcgtggc gatagagtga ctatgccttt tgtcgcagga tgtggccatt
     3781 gcttaccatg tctcactgga aatccccaag tctgcgacca ccagtttcaa ccgggattca
     3841 ccgcctgggg ttcgtttgcg gagtttgttg cgattcgtta tgctgatatg aaccttgtgc
     3901 gtttgcccaa tactatggat tcctccactg ccgcgagcc
//
LOCUS       DAOMZL010000124         3923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_21934_length_3923_cov_22.300931, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000124 DAOMZL010000000
VERSION     DAOMZL010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21934_length_3923_cov_22.300931"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLQ30_07820"
     CDS             <1..218
                     /locus_tag="PLQ30_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HPW02394.1"
                     /translation="FRAIINPPQCAIISVGAITRRPVEGNQENQYFTPIIEISLTADH
                     RIVDGAYGARFLEHFKDFIEQPVLALA"
     gene            356..1342
                     /locus_tag="PLQ30_07825"
     CDS             356..1342
                     /locus_tag="PLQ30_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPW02395.1"
                     /translation="MFQNELISPTVQMSEKPQPRRKTKKPPRFLCVCLNPTIQKTIVF
                     DKIEPGEVNRTSNWRIDAAGKGIIPTRILTQLGEKAIHLTQLGGPTRSWFLAMCAADG
                     ISVEWVESGAPIRFCTTLIEEAEGRITELVEESLPVASETSDDLVERFADLVNQADAV
                     LLSGTIADGIVQGTMARFARIAWDIGAHLYLDIKKQDLLDCLEYHPVCAKPNLEELAQ
                     TLGLPYAEVRKEDSARKLVEEQGKRFFDEYGTYLVVTRGAESTLYWDGQRLCEYPVQP
                     ITVRNPIGSGDAFGAGLARILERHGSIHEAVAEGTRLGGLNATQLRPGTLFS"
     gene            complement(1544..2851)
                     /locus_tag="PLQ30_07830"
     CDS             complement(1544..2851)
                     /locus_tag="PLQ30_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPW02396.1"
                     /translation="MAEQKKKGALDWYFKSNLLTRILIGLVLGAIVGIILGFFPSSVK
                     PYVDNTKFFGDLFIRLLKMIIMPVILFSLIAGAASIAPSKLGRVGVKIMVYYLLTSAF
                     AVIVGLISANIFKPGIGFNIVGEASVKGVQAAPPTIIQILLNIVPTNPFESLMKGDVL
                     PIIFFAVVFGIGISYIKDSKDQSLAKSGETLLNVVNAAAETMYKIVGGIMQYAPIGVF
                     FLISHVFAQQGAKAVGPLLMVTLSEYVGLAFHVVLVYGGLLTLYKLSFKKFLKGANEA
                     MVTAFVTRSSNGTLPVTMRCGEENLGIPRSISAFTLPLGATINMDGTAIYLGVCAMFI
                     GYATNQPLSFNQMLTVVITGTLASIGTAGVPGAGAIMLLMVLESIGLKVEAGSAVAAA
                     YAMILGIDALLDMGRTCLNVTGDLVGTSIVCKTEKELDLSKWK"
     gene            3020..3736
                     /locus_tag="PLQ30_07835"
     CDS             3020..3736
                     /locus_tag="PLQ30_07835"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HPW02397.1"
                     /translation="MKKATIGILGGMGPEATASFFSMLVRLDRATSDQDHIHIIVDSD
                     PSIPDRTKYLLEGGANPLPAMLGSARRLLAAGADIAGMPCMTAHALLDQLRHNCTLHF
                     LSAFEETGLAIRELSPPIRNLGVLATMGTRQAKLFETAFPDKNIIWPDEALHLSLVME
                     AIYGKRGIKAGYLGEESKHLLLEAVGFLKKHGAEAIVAGCTEVPLVLSQKDLDMPFID
                     PMYYLANALIATSRKGVLER"
BASE COUNT         1088 a          964 c          886 g          985 t
ORIGIN      
        1 gattcagagc catcattaat ccaccccagt gcgcaattat ctctgtagga gccataacaa
       61 gacgaccggt agagggcaat caagaaaacc agtattttac tcccataata gagattagcc
      121 ttacggccga tcatcgaata gtagatggcg cttatggagc tcgatttcta gagcatttca
      181 aagatttcat agaacagccg gtactggcat tggcttaatc gagcaataag ggattcaaat
      241 cgtcttaaat catatcgccc cgctcaaggc tcggggcgat tttttattcc ctgtccttgc
      301 atggcagcga gataaaacgc tttataatga taggattata tggtagattg gttctatgtt
      361 tcagaatgaa ctgatttctc ccacagtgca gatgagtgaa aagccacaac cacgacgaaa
      421 aaccaagaaa ccccctcgtt ttctctgtgt ctgcctgaat ccaacaatac aaaagaccat
      481 tgtattcgat aaaatcgaac caggagaggt aaatagaacc tccaattggc gtattgatgc
      541 tgcaggtaag ggcattattc caacacgaat tctgactcag ctcggagaaa aggcgatcca
      601 tcttacacag cttggtgggc ctaccaggtc ttggttcctc gccatgtgcg cagccgatgg
      661 catctcggta gagtgggtcg agtctggagc acccatacgc ttttgcacga ctttgataga
      721 agaggcagag ggaaggatta ccgagcttgt ggaagaatcc cttccggtgg catcggagac
      781 tagcgatgac ctcgtcgaac gcttcgccga tttggtcaat caagccgacg ccgtactcct
      841 ttctggcacc attgcagatg gtatcgtaca aggcacaatg gcacgctttg cgcgaatcgc
      901 ttgggatatc ggcgcacatc tctatctcga catcaagaaa caagatcttc tcgattgtct
      961 cgaatatcat cctgtctgtg caaagccaaa tctcgaagaa ctcgcacaga ctcttggact
     1021 tccttatgcc gaggtgcgta aagaagactc tgcccgcaag ttagtcgaag aacaaggcaa
     1081 acgctttttc gatgaatatg gcacctacct cgtagtaact cgcggagcag aatctacgct
     1141 atattgggat ggccaaaggc tatgcgagta tcctgtccaa cctatcacag tccgcaatcc
     1201 cataggctca ggcgatgcat ttggtgcagg ccttgcacgc attctagaac gccatggctc
     1261 tatccatgag gcagtagcag aaggtacacg actcggaggc ctaaacgcca cccaactcag
     1321 accaggaact cttttctctt gaaatcctcg aaatcccaat gaagaaattc atcgaggatg
     1381 ggctctctcg ataacaagtg caccctaagc aggcgccttg agatgaccca gctctataaa
     1441 aagggcgccc gcaatagcag acgccctaaa ctctataaac tatgcaaaag aacttattac
     1501 taggcgaact tcaatatggc tcgatatctc tcatttcctc ctcttatttc cacttcgaca
     1561 aatctagttc cttttctgtc ttgcagacaa tcgacgttcc aacaaggtct cctgtcacat
     1621 tcaaacaggt tcggcccatg tcgagcaaag catcgatgcc caagatcatc gcatatgcag
     1681 cagcgacagc tgagccagct tctaccttga gccctatgct ttcgagcacc atgagcagca
     1741 tgatggctcc agcgccaggt actcctgcag ttccgatact tgccaatgta cccgtgatga
     1801 ctactgtgag catctgattg aaggagagcg gttgattcgt tgcataacca atgaacatgg
     1861 cacagacgcc taagtatatc gccgtgccat ccatgttgat ggtcgcaccg agtggcaaag
     1921 taaaagcgct tatagacctc ggaatgccca gattttcttc accacagcgc attgttaccg
     1981 gtagagtgcc attggatgaa cgcgttacga acgcagtgac catcgcctca ttagcccctt
     2041 tgaggaattt cttaaaactg agcttataca atgtcaagag accaccataa accagcacaa
     2101 catggaacgc aagaccaaca tactcagaga gagtaaccat gagcaaaggt ccaactgcct
     2161 ttgcaccctg ctgagcaaat acatgcgaaa ttaggaagaa tacaccgata ggcgcatatt
     2221 gcatgatgcc acccacgatc ttgtacatag tctcggctgc tgcattgacg acattgagca
     2281 gcgtttctcc cgattttgcc agcgattgat cttttgaatc cttgatgtag gagatgccga
     2341 tgccgaatac cactgcgaag aatataatag gtagcacatc ccctttcata agggactcga
     2401 atggattggt gggaacaatg tttaacaaaa tctgaataat agtcggagga gcagcttgta
     2461 cgccttttac cgaagcttct cctacaatat taaaccctat tccaggctta aaaatattgg
     2521 ctgatatcaa cccgactatt acggcaaaag cgcttgtgag aagataatag accatgattt
     2581 tcacgcctac tcttccgagc ttagatggtg cgatactcgc ggctcctgcg atgagcgaaa
     2641 acagaattac aggcatgata atcattttga gtaacctgat aaacaggtca ccaaaaaatt
     2701 tcgtattgtc gacatagggt tttaccgagg aaggaaagaa tcccagaata ataccgacaa
     2761 tagctcccaa caccaggccg atcagtattc gcgtcaatag attggactta aagtaccaat
     2821 ccaaggcgcc cttcttcttt tgttcagcca tgcagacctc cttgctttat tgatgttcat
     2881 aaagccatat gattatggat ccgaattcgc cagaccgcaa gagggaaaaa tcttcaagct
     2941 ataatttcat tgcagtggta tttggtatag gcatctccta atttacatgg atccctgtgt
     3001 tatgtaacat gaatacgcta tgaaaaaggc aacaatcggt attctgggtg gaatggggcc
     3061 agaggcaacg gctagcttct tttcgatgct agttaggctc gatagagcaa cttccgatca
     3121 agatcatatt catatcatcg tggattcgga tccttcgatt cccgatagga caaagtatct
     3181 tctcgaggga ggcgcaaatc ccctccctgc aatgcttggc tcagccagaa ggttgctcgc
     3241 cgcaggcgct gatattgctg gaatgccctg catgacagcc catgcacttc tggatcaact
     3301 ccgccacaac tgcaccctgc attttctctc ggcctttgaa gagaccggcc tcgctattag
     3361 ggaattgtct cctcccattc gaaatcttgg tgttcttgcc acgatgggaa cgcgccaggc
     3421 aaagcttttc gaaacggctt ttccggataa gaatataata tggcctgatg aagccctaca
     3481 cctttctttg gttatggagg caatctatgg taagcgcggt atcaaggcag gctatctagg
     3541 cgaagagtca aagcatctct tgctggaagc ggttggcttt cttaaaaagc acggcgcaga
     3601 ggctattgta gcaggctgta cggaagtgcc tcttgttctg tctcaaaaag acctcgatat
     3661 gccattcata gatcccatgt attatctagc gaacgcgctg atagcaacat cccgcaaggg
     3721 tgtccttgaa agataattgg agctggtgaa aggatattcg atcgagatag atgcagtccg
     3781 gacacttgct ccagtttact catgtgtgct tttctcatat atttgaatga caatgctgtt
     3841 ccgagatcgc tgtgatggaa tacgcgtaac tggccaacat gctctcaatg cgctaatgcc
     3901 atatatgatg cgtggccgca atg
//
LOCUS       DAOMZL010000125         3795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_23001_length_3795_cov_11.675134, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000125 DAOMZL010000000
VERSION     DAOMZL010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23001_length_3795_cov_11.675134"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLQ30_07840"
     CDS             <1..251
                     /locus_tag="PLQ30_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HPW02398.1"
                     /translation="KKLNPKYVMSVLIPAAFLWITVTVALIWYEIVIVPVFFADATKT
                     MNIITGVIVGVINLLMLILNFMMINSFLKNYKLKPETA"
     gene            512..793
                     /locus_tag="PLQ30_07845"
     CDS             512..793
                     /locus_tag="PLQ30_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02399.1"
                     /translation="MPKRFGRIIKNIFKTFAQVNREKATGMLDFELKELENIFALLIL
                     GGFVGLPSPPSPIAIELLPYMERELIVLLSRSDLSQDPLGVLASMLEID"
     gene            803..1735
                     /locus_tag="PLQ30_07850"
     CDS             803..1735
                     /locus_tag="PLQ30_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HPW02400.1"
                     /translation="MRDNIFFLGKGGVGKTTVSSAFAMALARRGSRVLAVSLDPAHNL
                     GDALQTKLNGEPKKVEPMLDALEVDLAYWVDRYLEDSKEELKANYSYNSALNLDSFFG
                     ILKYSPGTEEYAVLWAIEDIRRRLAPQYDFVVFDTPPTALTLRFLSMPAISGLWVAEL
                     TKLRERILRDRQTIVRLNPQSPVAESCVDKEDDKVYGKLKNIRTRLTSLQDLFAKRSY
                     MSIIVNPDELSVSEAIRIKDELSQLGIMLSAVCLNKKGLSRAEWTLSPEFGQLPLLIC
                     DFAPNGISTREDLVHIGIEPFIDLFLGKRYVKNE"
     gene            1728..2381
                     /locus_tag="PLQ30_07855"
     CDS             1728..2381
                     /locus_tag="PLQ30_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02401.1"
                     /translation="MNNFLFFAIFALGVVATIQYFLGVKKNRWMGKNLSSQAEGILKP
                     KDTDYVNIGGAIGYNFAYKLKEPWKNAKGTFTLFPRHSLLYMPISLVVGNCDRFYMNI
                     FTDKKLVGEGHIIEKRHSRRAKIEGLEEMHKEEIQRAGKTFVLCWRSTNLRDKLIQTL
                     DSMPDASSLTHFCCYSENKTFFLYLKPKKGQISDNLKAFLVQCPTYFRQENPRESNK"
     gene            2526..2798
                     /locus_tag="PLQ30_07860"
     CDS             2526..2798
                     /locus_tag="PLQ30_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02402.1"
                     /translation="MDPEMKKKIDETLAQVKEPQSEEPIVNLGLLEKVTYSEKEKTML
                     VKLAIGTPRWQCPACSAINGVVKAGIVRRAQEAFEKAFPDLKIIVE"
     gene            complement(2866..>3795)
                     /locus_tag="PLQ30_07865"
     CDS             complement(2866..>3795)
                     /locus_tag="PLQ30_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPW02403.1"
                     /translation="APELNGMKLYKNKLMRPLILRIAGMKLKKTFGGHLRFFGIGGAP
                     LAAEVEEFLKKAHFPYAIGYGLTETAPLLAGCAPKVTFLRSTGPALKGVEVRIADPKT
                     ITGEGEIQARGPNIFKGYWRDEVRTKEAFTEDGWFRTGDLGSIDNKGRLFVRGRLKTM
                     ILGASGENIYPEEIESILNQMPEIEESLVVEEEEGLTALVCLKSEVLDNIGARLQDHL
                     DAVGDLSSRVGQAIAQAEKSVTGSVNQALTDTEKAIERLLESIRKEANSRLAAFSRIQ
                     HVKFHPEPFEKTPTQKIKRFLYGKKGEKPELAQ"
BASE COUNT         1069 a          858 c          870 g          998 t
ORIGIN      
        1 agaagaagct caaccccaaa tatgtcatgt cggtattgat tccggctgct tttctttgga
       61 taactgttac agtagcgctc atctggtatg aaatcgtcat agtccctgta ttctttgcag
      121 atgccacaaa gactatgaac ataattactg gcgttattgt aggagtcatc aacctcttaa
      181 tgctcatact caacttcatg atgatcaact cattcctaaa gaactataag ctgaagccag
      241 agacagcctg ataaatggta atttccgcct aattttggtc cgttggattg caattatggg
      301 cggatgacaa aaacgaagag ccggaagcca tgtgcttttc ggctcttttt ctgttttgag
      361 ctatgagcta tggcacattg aacaaattag ttgagatgcg ataagaagta gaaactcgaa
      421 gcttgtcaat cttgctatga gcgcgctata gttaggcaat catagttgag ccaagagaag
      481 tcttatatag atgagcccga aggaagcaaa aatgccaaaa cgctttggcc gcataatcaa
      541 gaatatattc aaaacctttg ctcaggtaaa cagagaaaag gcaaccggga tgctcgattt
      601 cgagctcaag gagctggaga atatctttgc gcttcttatt cttggaggct ttgtaggcct
      661 gccttcgcca ccatcgccta ttgccataga attattgccc tatatggaaa gagagctgat
      721 tgtacttctc tccaggtcgg atctatctca ggaccctctg ggtgtattgg ctagtatgct
      781 cgaaatagat taaggagtct ctgtgcgcga taatattttc ttccttggaa agggaggcgt
      841 aggcaagacg acggtatcca gtgcctttgc catggctctg gcaagaagag gcagtagagt
      901 ccttgcggta tcgctggatc ctgcgcataa cctgggagat gctcttcaga caaaattgaa
      961 tggagagccc aaaaaagtcg aacccatgct agatgcgcta gaagtcgatc tagcatattg
     1021 ggtggataga tatctcgaag acagcaaaga agaactcaaa gccaactatt cttacaattc
     1081 agcgctaaat cttgattctt tctttggcat tctaaaatat tcaccaggta cggaagaata
     1141 tgcggttctg tgggcaatcg aagatattcg ccgcagactt gcgcctcaat acgattttgt
     1201 ggtcttcgat acgcctccga ctgctcttac gcttcgcttt ctttctatgc cggctatttc
     1261 aggcctttgg gtagcagagc taacaaaact gcgtgagcga attcttcgcg atcgacagac
     1321 aatcgtccgc ttaaatcctc agtcgccggt tgcagagagc tgcgtggaca aagaagatga
     1381 taaggtctat ggaaagctca agaatataag aaccagactt acatcgttgc aggatttgtt
     1441 tgcaaagcgg agttatatga gcatcattgt aaaccctgat gagctttcgg tgtcggaagc
     1501 cataagaatc aaagacgaat tgtctcagct ggggattatg ctctctgctg tatgcctgaa
     1561 taaaaaaggc ctttcgaggg cagaatggac gctctcgccc gagtttggcc aattgcctct
     1621 attaatctgt gattttgcgc caaatggcat tagcaccaga gaagacttgg ttcatattgg
     1681 catcgaacca ttcatagatc tttttctagg caagaggtat gtaaagaatg aataattttt
     1741 tattcttcgc gatatttgca cttggggtcg tcgcgacaat tcagtatttc cttggagtaa
     1801 agaaaaaccg ctggatgggg aaaaacctct catcgcaagc agagggaatc ctaaaaccga
     1861 aagatactga ttatgtcaac ataggcgggg cgattggtta taattttgct tataagttga
     1921 aagaaccatg gaaaaatgca aagggcactt tcacgctgtt tcctcgccat tctctcctat
     1981 atatgcccat ttcccttgtt gtgggcaact gtgatcgctt ttatatgaat atctttacag
     2041 acaaaaagct ggtgggagaa ggtcatatca tcgaaaaaag acactctcgc agggctaaaa
     2101 ttgaaggcct agaggaaatg cacaaagagg aaatccagcg cgctggaaag acatttgtgc
     2161 tctgctggcg ctcgacaaat ctaagagaca aacttatcca gactctcgat tccatgcccg
     2221 atgcctcgtc gctaacgcat ttctgctgct actcagagaa taaaacattc tttctttact
     2281 tgaagcccaa aaagggccaa atatcggaca atctaaaagc ctttttggtg caatgtccca
     2341 cttatttccg ccaggaaaat cctcgcgaat caaataaata ggatgctatg cttgatctgt
     2401 gcagccctaa ggaagctccc taaggagtgc gaggatagcg ataaccagcg cccgcaatgt
     2461 attatatgcc agctgcataa gcaaataagg caagagtaga caaaaggaag taaaaggagg
     2521 cgcctatgga ccctgaaatg aaaaaaaaga tcgatgagac cttggcccaa gtaaaagagc
     2581 cacaatccga agagccaata gtaaacctcg ggcttctaga gaaagtcacc tacagcgaga
     2641 aagaaaaaac aatgttagtc aagcttgcca tcggtactcc tcgatggcaa tgcccagcgt
     2701 gctccgcaat aaatggagtt gtaaaggcgg gcattgtacg cagggctcaa gaggcattcg
     2761 agaaggcatt ccctgatttg aagataatcg tggaataaca aagcagaaca gcaatctata
     2821 atctatgaca aaataaatat tgtagccgcc gctatgctcg catatttatt gggcaagctc
     2881 tggcttttca cccttctttc catataagaa gcgcttgatc ttctgagtcg gcgttttttc
     2941 gaagggctcg ggatggaatt ttacatgctg aatgcgtgag aaagcggcaa gccttgagtt
     3001 cgcttccttg cggatgctct ccaaaaggcg ctcgatagct ttctcggtat ctgtcaaggc
     3061 ttggtttacg ctgcctgtaa cggatttttc agcttgggcg atagcttgtc caacacgcga
     3121 actcagatct ccaacggcat cgagatgatc ctgcaaacgc gcaccaatat tatccaggac
     3181 ctcgctttta aggcatacta gcgcggtaag tccctcttcc tcttcaacca caagggattc
     3241 ctctatctca ggcatctgat tgaggatcga ttcgatctct tcgggataga tattctcgcc
     3301 cgatgcgcct aagatcattg tcttgagtcg tccgcgtacg aaaagccgac ctttattatc
     3361 gatcgatcct agatctcccg tgcggaacca tccatcttca gtgaaagctt cttttgtccg
     3421 aacctcatct ctccagtagc ctttgaagat attggggccc cttgcctgga tttcgccttc
     3481 tccagtaatg gtcttaggat cggctatgcg cacctctact cccttgagcg ctggacccgt
     3541 agaacgcaaa aaagtcactt taggagcgca acccgcaaga agaggagcgg tctcagtaag
     3601 tccatagcct atcgcataag ggaaatgagc tttcttgagg aattcttcga cctccgccgc
     3661 cagaggcgct ccgccaattc caaaaaagcg cagatgacca ccaaaagtct tctttaattt
     3721 cattcccgca atgcggagta tcaaaggccg catgagcttg ttcttgtaca atttcatccc
     3781 atttagctcg ggagc
//
LOCUS       DAOMZL010000126         3704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_23820_length_3704_cov_8.967662, whole genome shotgun sequence.
ACCESSION   DAOMZL010000126 DAOMZL010000000
VERSION     DAOMZL010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23820_length_3704_cov_8.967662"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLQ30_07870"
     CDS             <1..427
                     /locus_tag="PLQ30_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02404.1"
                     /translation="TINITANVSQILDVSVDPPTWTIPLDNSGSAIQKDDQGTFTVKA
                     SKSNYTVTFSSGHSGTLVNGFEAIPYKIKVDTAGWNGVATNNLNSYTQLIGAKTIVFN
                     KKTPTAGKTFNIGFNIEAYTDYYIDGEYTDTLTISIAVS"
     gene            496..1011
                     /locus_tag="PLQ30_07875"
     CDS             496..1011
                     /locus_tag="PLQ30_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02405.1"
                     /translation="MKKWVLLVFALSMILKLNAQASIGITVVVAPPPGASITMDSYIW
                     EISFANDGVTTYQNDNQGTMTIRSSNRKNFRVDFSSQNLGRLKQGTDYVAYYIKATAI
                     TGGNIGLVGTPAIVAGYVQLTSVQSMEFNKKTPKKGIQFYTGIKIDPILGEFYESGSY
                     TDTLTITFVGL"
     gene            1912..2415
                     /locus_tag="PLQ30_07880"
     CDS             1912..2415
                     /locus_tag="PLQ30_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02406.1"
                     /translation="MKNKVFLSGLVLLLIAIPVFASGSSTINLDATVSQILSITKCSG
                     FTETLDTTNAGAINGVCLGAVGIKANVQNWEIQIKSDNTWKMVKAAGQPGSDSGYPYE
                     FRFGDTGETIVNSTSEPDGLYTIQKNAPLTTEGYFNIFVFYQTAEALGILAGSYTDTI
                     TITLSAT"
     gene            2534..>3704
                     /locus_tag="PLQ30_07885"
     CDS             2534..>3704
                     /locus_tag="PLQ30_07885"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HPW02407.1"
                     /translation="MSIVLLSLLFCVSAIFFFVMLYKSANRSESELLGSVSKRIAQQI
                     SESLQEMLNVGEGLRELIIAGSGRNYASLGPLADEALARLPYIDSITIAPGAIVRYFF
                     PESRASASIGHDLLDNPDRMRALVEAVRKKSAVLQGPELSAEGKNLAFLRIPVIEGSE
                     LWGFVSIAFDADKLLSDLDLVSQFPGLSIAVASSDMEKGDKELFWGEESALSGYSAGL
                     KIGSEDFSWTIYVASLYPYRQVALWGAGLLILVLLSCGLAILAGSLEKEGKAPIGSKE
                     ALITIKPFVPKTEPLQESSIEVIKKAEILQEPLAENLVSASLGRPIKETVEGPAQEKI
                     ILRDQEIENEAENKESAAPKEKIASARSVLVVDDSEVNRDLLLRMLTLKGYEARAV"
BASE COUNT         1085 a          721 c          900 g          998 t
ORIGIN      
        1 tacaatcaac atcacggcaa atgtttcgca aatacttgat gtgagcgttg atccgccaac
       61 ctggactatt cccttggaca actcaggatc agcgattcaa aaagatgatc aaggcacatt
      121 cactgttaaa gctagcaaat cgaactacac cgtgaccttt tcttctggac acagtggcac
      181 tcttgtgaat ggattcgagg cgattcctta caaaatcaag gtagacaccg ccggatggaa
      241 cggagtagca acaaacaacc tgaactcgta tacgcagtta attggtgcga aaaccatagt
      301 ctttaacaag aagaccccta ctgcaggtaa gacattcaat atcggtttta atattgaagc
      361 ctataccgat tattacatcg atggagaata tacggatacg ctaactatca gcattgctgt
      421 ttcgtgatca aagagtacaa gcgctcctta attttcgctt ccttttggcc ttaattaagg
      481 ctagggacaa tcaaaatgaa aaagtgggtt cttctggttt tcgccttgtc catgatactt
      541 aagctaaatg cgcaggccag catagggata acagtagtag ttgctccccc tccgggagca
      601 tctattacta tggactctta tatttgggaa atctcgtttg caaatgatgg agtcactacc
      661 tatcaaaatg ataatcaagg aacaatgact attcgctcaa gcaatagaaa aaattttcgt
      721 gtcgattttt catcacaaaa tcttggacgt cttaaacaag gcacagacta tgtagcatat
      781 tacatcaaag cgacagccat taccggcggc aacataggct tagtgggaac acctgctatt
      841 gttgctggat atgtgcaact taccagcgta cagagtatgg aattcaacaa aaagacaccc
      901 aagaaaggta ttcaattcta taccggaata aagatagatc ctattttggg agaattttat
      961 gaatctggaa gttacactga tactttgact atcacttttg ttggactgtg aactattatt
     1021 gaagatcatg gattgaggca ggaataaaag ccgctatcaa gcagaagctt gatggcggct
     1081 ttttagatgt gtctaaaaca acgcatctat tcgcgattga gtcgatgaac ttcttcttgt
     1141 tgagcttcct agaagtagtt tatatagggt acttctagaa gggttttatt atgaagaagg
     1201 agagaactca tcctgtatta gtttctgccc atgttgaaac cgcatgataa tgcccctggc
     1261 ggatctaccg gcgatatctg gaggaaggcg acgaagggtt gctgcaccgt aaccaaggga
     1321 aggcctcagg catggcttat gatgtgggga tacgaggggc ggcggtgcgt ctttgctagg
     1381 agtactatgg tactatggag actttgggcc tacgtttgtg gcagagacga agaagtgcct
     1441 gctgcctgat gaatatggtg gatgaccaac agaaatcacc ttctcatttt tctgtgaaga
     1501 ataaaccacg tccgatgcga tgcgactcct ccggcgctgg attgagcgtt atggcataca
     1561 acaggcgaaa gggcgggttg aacgaagcca tagagtgtat caggaacggc tggtgaaaga
     1621 actgaggctg gctggtatct cgacgattga ggatgccaat cgctatctca gggaagtcta
     1681 tctttctacc aaactataga actcgagtat gtcgaaatcg agcaactacc acacaaggag
     1741 gaactggtca ctcgctcagc ctgagtgaaa aggggacatt ttaatcgagc tatatatgtg
     1801 gacattttaa tcgagtcttg gtataagtca aaaaggatca ttgataaaat gtcaaatttg
     1861 gctaatttat gaaaaaattt taggctatta ttggcctaaa ggagaaagtt tatgaaaaat
     1921 aaagtgtttc tatcgggact agtgttattg cttattgcta ttccagtctt tgcttcagga
     1981 tctagcacaa tcaatttgga tgcaaccgtc agccagatac taagtatcac taaatgctct
     2041 ggttttactg agactcttga tacgactaat gcaggtgcaa ttaatggtgt ttgcctcggt
     2101 gccgtcggaa taaaggccaa tgtacaaaac tgggaaattc agattaaatc cgacaataca
     2161 tggaaaatgg ttaaagcggc aggacagcca ggaagcgata gcggttatcc ttatgaattt
     2221 agatttggcg atacaggaga gacgatagtt aattctacca gcgaaccgga tggattgtat
     2281 actatacaga aaaatgcgcc attgaccact gagggatatt tcaacatttt tgtattctac
     2341 cagacagcgg aagcgctcgg tatcctggcg ggttcttata cggatactat tacaattaca
     2401 ctttcagcaa cctgagagaa ttatcctgca tagttataga aggctgcccg cttaatgggc
     2461 ggcctttttt atgatatttt tatataattg aattatgagc tttattagca gccagaaaaa
     2521 gaatatagcc aagatgtcaa tagtcttgct ttcgctattg ttttgcgtgt ctgcgatatt
     2581 cttttttgtc atgctataca aaagcgcgaa ccgatcggaa tcagaactgc ttggaagcgt
     2641 ctccaagaga atagcgcagc aaatctccga gtccctgcaa gagatgctca atgtcggtga
     2701 aggcttacgc gagttaatca ttgctggtag cggtaggaac tatgcgtcgc tgggaccact
     2761 ggccgatgag gcgcttgctc ggttgcctta tatagactcg attacgatag ctccgggggc
     2821 aatagtaaga tatttctttc ctgagagtag ggcttcagct tcgattggtc acgatttgct
     2881 cgataatcct gatagaatgc gtgctctggt cgaagcagtg cgaaagaaga gcgccgtttt
     2941 gcaggggcca gagctatccg cagaaggaaa gaacctcgca tttttgcgaa tacctgttat
     3001 agagggctcc gaactttggg gttttgtgag tatcgcattc gatgcagaca agctattgtc
     3061 ggatttggat ttggtttcgc aattcccggg gctgtccatt gccgttgcat cttccgatat
     3121 ggaaaaggga gacaaggagc tattttgggg cgaagagagt gcgctttccg gttattcggc
     3181 tgggctgaaa atcggatcag aagatttttc gtggactatt tatgtagcat cattgtaccc
     3241 atatagacag gtggcgctat ggggagcggg acttcttata cttgtgctat taagctgtgg
     3301 gcttgccata ctagcaggat ctttagagaa agagggcaaa gctcccatag gatccaaaga
     3361 agcacttata accataaagc ctttcgtgcc aaaaacagaa cccctgcaag agtcttctat
     3421 agaagtgatt aaaaaggccg agattctgca agagcccctg gcagagaact tggtaagcgc
     3481 ttcgttagga aggccaataa aagaaacggt tgaaggacct gctcaggaga agattatatt
     3541 acgggatcaa gaaatcgaaa atgaggccga aaacaaagaa agcgctgcgc ctaaagaaaa
     3601 aatcgcgagt gccagatcgg tgcttgttgt cgatgatagc gaagtaaatc gcgatcttct
     3661 tttgcgaatg ctcacattga aaggttatga ggcaagggct gtct
//
LOCUS       DAOMZL010000127         3623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_24612_length_3623_cov_12.348935, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000127 DAOMZL010000000
VERSION     DAOMZL010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24612_length_3623_cov_12.348935"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PLQ30_07890"
     CDS             <1..752
                     /locus_tag="PLQ30_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPW02408.1"
                     /translation="KDAALERTARGIINSAFGCAGERCMALPVIVVEEEIADELVGIL
                     KEQSKNLKVGPAYDKTSQMGPVVNAEHKASIVKWIEKGIEEGAELVLDGRDIVIEGYE
                     NGFYLGPTILDHVTPEMSVGWKEIFGPVLCVKRVKDFEEGLELMNSSEFGNGSVIYTQ
                     SGYYAREFAKRTHGGQVGINVGIPVPLSVFGFTGHKNSFFGDLHTMGTDGVRFYTELK
                     SVTSHWFSEEEAREGKVVNSWDGTISFPDKK"
     gene            complement(875..1732)
                     /gene="panB"
                     /locus_tag="PLQ30_07895"
     CDS             complement(875..1732)
                     /gene="panB"
                     /locus_tag="PLQ30_07895"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPW02409.1"
                     /translation="MAEEMQKLSIPDLVSMKREGKKISMITAYSYPQGLLVDEAGIDI
                     ILVGDSLGMVELGYSGTTPVTMEEMLAHIKPVTRAVKRAHVVGDMPFMSYNVSIEQAI
                     TNAGILYKEGACDSIKLEGGQEMAKTIEAIIRAGIPTFGHIGLTPQTAGMLGGFKVQG
                     KSLDAAKKLIDDAIAVDEAGAFAIVLESIPRQLAKIISQKVKAITIGIGAGMECDGQV
                     LVFHDILGLFKRFTPKFVKVYANAWDYELKAIKDFIDEVKEQKFPTDEFTYTMKEDVL
                     NELSKYAGV"
     gene            2075..3352
                     /locus_tag="PLQ30_07900"
     CDS             2075..3352
                     /locus_tag="PLQ30_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPW02410.1"
                     /translation="MNTEEKYKNYVNTSFVKAIEPVVVTNASGSKVTSEDGRVYTDLY
                     SGISVVNAGHCNPEVAQAAKAQIDKLIHCGTYVYYSPTAADFAEKLAEVAPGRLQKTF
                     FGNGGAEANECGMRMAKQYTKKQEFIALHGSFHGRSLATLSITGNKGRKKGGGPYIPG
                     VAFNPAPYCYRCAFNLSYPSCDLYCAEYLERTIQYDTSDNVAAFVAETIMGEGGIIVP
                     PKEFFKEIKKILDQYGILLFLDEVQAGFGRSGKLFAIEHYDVEPDIMTMAKGIAAGFP
                     LSACIARPEIADSFRPGDHLSTFGGNPVSCAAGIANLNYMLREKLPEKAMEKGRIFME
                     ELKEIQKTTSLIGDVRGSGLMIGVELVRDKRKTPAAEEAAKIKTLMREKGFLIGLGGT
                     FANVLRLQPPLVISSEELSSAAKALSECFRELT"
     gene            3349..>3623
                     /locus_tag="PLQ30_07905"
     CDS             3349..>3623
                     /locus_tag="PLQ30_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HPW02411.1"
                     /translation="MKISVIGSGAMGSLFGGRLALAGENVVLYDVYREHIDAVNTQGL
                     SIEDAETGEITIAHPQASSDPESVKNSDVFLIFVKSTNTESVANQFKS"
BASE COUNT         1015 a          707 c          876 g         1025 t
ORIGIN      
        1 tgaaggatgc ggccttagag aggacggctc gagggataat caattcggca tttggctgtg
       61 cgggtgagcg ttgtatggcc ttaccagtga tagtggtgga ggaagagata gcggacgagc
      121 ttgtagggat tctcaaggag caatcgaaga atctcaaggt agggccggcg tacgacaaga
      181 cgagccagat ggggccggta gtgaatgcgg agcataaggc atcgattgtg aaatggatag
      241 agaaaggaat agaggaagga gccgagctcg tactggatgg tcgagatatc gtaatagaag
      301 gctatgagaa tggcttttat ctagggccta cgattctgga tcatgttaca ccagagatga
      361 gcgttggatg gaaagagatt tttggtccgg tactctgtgt aaagcgagtg aaggatttcg
      421 aggaaggcct ggagctcatg aactcgagtg aatttgggaa tggttcggtg atatatacac
      481 agagtggcta ttatgccagg gaatttgcta agaggacgca tggaggtcag gtaggtatca
      541 atgtaggtat tccagtaccc ttgagtgtat ttggttttac cgggcataag aattcgtttt
      601 ttggggatct ccataccatg ggtacagatg gagtaagatt ctacacggag ttgaaaagtg
      661 tcacttctca ctggttctcc gaagaggaag cgagagaagg caaggttgtc aacagttggg
      721 atggaacaat atcttttcca gataagaaat aaaagacaaa aatccttcag tctgtaattt
      781 agaaatgcct gcagagttca gtgagcccta caggcatttt tgatttccaa gatatctcaa
      841 ttctcaattt ccttgtaaac agcactcttt gggcttagac tcctgcatat ttgctgagtt
      901 cattgagcac atcttctttc attgtgtagg tgaactcatc ggttgggaat ttttgctctt
      961 tcacctcatc aataaaatct tttatggctt tgagttcgta atcccatgca tttgcataca
     1021 ctttgacaaa tttcggggtg aatctcttga agagccccag gatgtcatgg aagacaagca
     1081 cctggccatc gcattccatg ccagcaccga tacctatggt tattgctttc actttttggg
     1141 agatgatttt tgctagttgc cgaggaatag attccaggac tattgcaaat gcgccagcct
     1201 catcgactgc tatcgcatca tcgatcagtt tctttgcggc atcaagggat ttgccttgta
     1261 ctttgaagcc gccgagcata cctgctgtct gaggagtcag cccaatgtga ccaaaagtag
     1321 gaatacccgc gcgaattatt gcctcaatgg tctttgccat ttcctgtccg ccttcgagct
     1381 tgatcgaatc gcaggctccc tctttatata gaatacctgc atttgtaatt gcctgttcta
     1441 tggagacatt atatgacatg aatggcatgt ctccgaccac atgagccctt tttacggctc
     1501 tggtgaccgg cttgatatga gcgagcatct cttccatggt cacaggtgta gtcccactat
     1561 aaccaagttc taccatacca aggctgtcgc caactagaat aatatctatg cctgcttcat
     1621 ctacaagcaa cccttgagga tatgaatatg cagttatcat cgatatcttc ttgccttctc
     1681 tcttcattga gacaagatca ggaatagata gcttttgcat ttcttccgcc attgctagac
     1741 ctccgttagt tgaaatagat acctcatcat atatcgaaat agccagccat acaatggcaa
     1801 aaccatttca atactcccgt aaatcattat atcttttcca aataaaagct tgactttgct
     1861 atcaactatg gtaacatatt aagactatcg atgaagatag attatgcata atgttcgata
     1921 tttatcactg tgatcgaagg aatcttgata agaaagggag gcagatcaat tatataagag
     1981 gcatgatctt ttttggttcg acagtgcttg aggaatgatt ggagttctgg tctgaattta
     2041 caatatttgc ccaaataaaa ggaggtctct aaacatgaac actgaggaga agtataagaa
     2101 ttacgtaaat acttccttcg ttaaggcaat tgagcctgtt gttgttacta atgcttcagg
     2161 cagcaaagta accagcgaag atggcagagt atatactgat ctatattcag gtatttcggt
     2221 agtgaatgcg gggcattgta atcccgaagt agcacaagca gccaaagcac agattgataa
     2281 actaattcat tgtggtacat atgtttatta ttctccgaca gctgctgatt ttgcagaaaa
     2341 gctcgcagaa gtagcacctg gaagattgca gaagaccttt tttggcaatg gaggagccga
     2401 ggccaatgag tgtgggatgc gcatggccaa gcaatatacg aaaaagcagg aattcattgc
     2461 actgcatggc tcctttcatg gaagaagttt ggccacattg tctataactg ggaataaagg
     2521 taggaagaag ggcggtggac catatattcc aggtgttgcc ttcaatccag ctccatattg
     2581 ttatcgatgt gctttcaact tgtcatatcc ctcttgcgat ttgtattgtg cagagtatct
     2641 tgagcgtaca atacaatatg ataccagtga caatgttgcg gcatttgttg cggaaacaat
     2701 tatgggagaa ggcggcataa tagttccgcc aaaagagttt tttaaggaaa tcaagaaaat
     2761 actggatcag tatggtatcc ttctgttcct tgacgaggtt caagctggtt ttggtcgctc
     2821 cggcaagcta tttgcgatcg agcattatga tgtcgaacca gatatcatga ccatggcaaa
     2881 aggcatagcg gcaggctttc ctctatctgc atgtatagca aggcctgaaa ttgccgattc
     2941 cttccgacca ggagatcatc tctctacttt tggaggcaac cctgtttctt gtgcagccgg
     3001 aattgcaaat ctgaattata tgctgagaga gaaacttcca gaaaaggcta tggaaaaggg
     3061 tagaattttc atggaggaat taaaggagat acagaaaacc acatcgctta tcggagatgt
     3121 tcgcggctcc gggctaatga ttggcgtaga gttggttcgt gataaaagaa aaacccctgc
     3181 agcggaagaa gcagccaaga tcaaaaccct tatgagagag aaaggatttc ttataggctt
     3241 gggtggcacc tttgcaaatg tcttaaggtt gcagcctcct ttggtaattt cttcggaaga
     3301 actttcttct gctgcaaagg ctctttctga gtgctttaga gaattgacat gaaaatctct
     3361 gttattggct caggagccat gggttctctc tttggagggc ggcttgcttt agcgggagag
     3421 aacgtagttc tctatgatgt ctatagagaa catatagatg cggttaacac acaagggctt
     3481 tcgatagagg atgccgagac tggcgagatt acgatcgctc atccacaagc atcctctgat
     3541 cctgaatctg ttaagaactc cgatgtattt ctgattttcg tcaaatcgac aaacacagag
     3601 tcggtcgcca atcaattcaa atc
//
LOCUS       DAOMZL010000128         3594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_24919_length_3594_cov_16.508618, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000128 DAOMZL010000000
VERSION     DAOMZL010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24919_length_3594_cov_16.508618"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3190
                     /locus_tag="PLQ30_07910"
     CDS             <1..3190
                     /locus_tag="PLQ30_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02412.1"
                     /translation="LKPACNARASHNCVHNMHSHKICTHKSHNKGFYNLRVIIALFFF
                     SAFVQTQAQLLPSIKPVLHFRTDCFDIYAPQSLEADAKRLAGFADNTYGELCEFLGLG
                     LETKRIPVLLSDIEYSLNGFSTLFPSNRIVLLLSAADPRDQLATFEDELYSVFLHELV
                     HYVTLNERSLAWRVLAWLGGDWIAPEIYMMPQAIVEGTAVWVESRFKENNSGRLNDPA
                     AMEFVRLERARGENRSLWDVSGLEDFYGAGSLPYLYGGLFVDYLSERFGKSIVARLWQ
                     SGGRGNIYQGFVGGILVKGILERETGIEFDQLWQDFLAWVDEESDLEMQGEQGEIDEI
                     GENSAAELPEGQELFSGYIGALGAGGGKVYFVDLERHGLYQLSLVDLEENSERQPDLE
                     KEKEEKSEVAEKQDKQEMLERLNKPKRPERLFAADQMLRNIYCDVNFGGLELDWNRIN
                     PQNQLIPARYLYDLSEHKLSYEKDLPQAKIGEALFETHSSSKEEIFLHDSWQDPLTSI
                     EYGLVRLGTAVLPARRLANMEIEVADIPDSAIRWLSQGFRDNSESSDSICFALSLVPD
                     NGLSRLAILEQDNGLWRFAIARKSPLGGVHMPVFADASHIVYKASSENGQSSLLLLDI
                     SSFRNEIETPGRFLDSEFLNWISPTEWIESYSLKMKNQSAHLDSTEEAQSQPELSKVE
                     QPRQETNKLAQPEKEFSLEPCSATQKATQKRSMLFPESFETSRIPYAYGTLLGIGFIA
                     MDLTERLSWSAFVGWDWLIARPASSIDLSLSAGAWSFEICAWDQGIFAFPVARISALE
                     GALDWEHTLVPTFRSISAGIHARYGAVQNKYEEQGLFHQSPDYFAWVAGTGVEFDSLS
                     SSYRPPYDPSGISLKGRIEYEGANSLKFGGVSLSGSIYLGESTIAGRIGASIYGAWAP
                     TGGVAFSPATRYLEYAGDHELSVADLPYPAYKEYQACSELSAWYLYGEIKARLFSLEL
                     NWIMSNLFAPSLALRRISMISGLRGAGLEIDNAPYLLSSAFLQAKFDCAFLSGVVAAT
                     HMKFTLEAAWAFQADKAGGEPFCFSVGLSGQF"
     gene            3381..>3594
                     /locus_tag="PLQ30_07915"
     CDS             3381..>3594
                     /locus_tag="PLQ30_07915"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HPW02413.1"
                     /translation="METSFSPPLDPCTYDLRIGTVGYEYSEWEGVLYPNGIEKETYLR
                     LYCERFSTLELDFTFHTMPKAAQMRDI"
BASE COUNT          954 a          759 c          883 g          998 t
ORIGIN      
        1 tcttaagcct gcttgcaatg cgcgtgcaag ccataattgt gtccacaata tgcatagcca
       61 taagatatgc acccataagt ctcataataa gggcttttac aatctgcgcg tcataattgc
      121 cttattcttc ttttctgctt ttgtacaaac ccaggcacag cttcttcctt ctattaaacc
      181 agtgcttcat tttagaaccg attgcttcga catatatgca ccgcaaagcc tagaagccga
      241 tgcaaagagg ctagccggct ttgccgataa tacctatgga gaactatgcg aatttctagg
      301 actagggcta gagacaaagc gcattccggt gcttttaagc gatatcgagt attccttgaa
      361 tggattttct acccttttcc cttccaatag aatcgtgctg cttttatctg ctgcagatcc
      421 gagggatcag cttgcaacct ttgaagatga gctatattca gtgtttttgc atgagcttgt
      481 tcattatgtc accctaaatg agcggagtct tgcttggaga gtactggcat ggctcggagg
      541 tgactggatt gctccagaga tatacatgat gccgcaggcc atagtcgaag ggaccgccgt
      601 gtgggtggag agtaggttca aagagaataa ttccggtagg ctgaatgatc cggcggccat
      661 ggaatttgtc aggctggaga gggcgcgtgg cgaaaatcga tcgctttggg atgtttccgg
      721 cttggaggat ttttatggag caggttcact gccatatcta tatggtggtc tatttgttga
      781 ttatttgagc gaacgttttg ggaaatccat tgttgcaagg ctatggcaaa gcgggggtcg
      841 cggaaatatc tatcaaggat tcgtcggagg aattcttgta aagggaattc tggaacgcga
      901 gacgggaata gaattcgacc agctatggca ggattttcta gcatgggttg acgaagaaag
      961 cgatttggag atgcaaggag agcagggcga aatcgatgaa atcggagaga acagtgccgc
     1021 agagttaccc gaggggcagg agcttttttc aggatatatt ggggctcttg gggctggagg
     1081 gggaaaagtc tattttgtgg acctagagcg ccatggcctt tatcagctct cattagtgga
     1141 tttggaagag aactctgaaa ggcagcctga tcttgaaaag gagaaggaag agaagtctga
     1201 ggttgcagaa aagcaagaca aacaagagat gctagaaagg ctgaataagc caaaaaggcc
     1261 agagagactt tttgcagctg atcaaatgtt acgtaacatt tattgtgacg taaatttcgg
     1321 aggactggaa ctagactgga atcgaatcaa tccgcaaaac caacttattc cagcacgcta
     1381 tctatacgat ctctctgagc acaagctcag ctacgaaaaa gaccttccac aggctaagat
     1441 cggcgaagca ttattcgaaa cacattcttc ttcaaaagaa gagatatttc tccatgattc
     1501 atggcaggac cctcttacgt ccatcgaata tggccttgta aggctgggca ctgcggttct
     1561 tcctgcccga agactcgcca acatggagat cgaggtagct gatattcctg attcagccat
     1621 acgatggctt tcccaaggtt ttcgagataa ttccgagtca tccgattcta tttgctttgc
     1681 cttgagcctc gtgccagata atggattatc gcggcttgct attttagagc aagataatgg
     1741 gctttggcga tttgcaatag caagaaaaag tcctctgggt ggtgttcata tgccagtctt
     1801 cgcagatgct tctcacattg tctataaagc atcctctgaa aatgggcaat cgagccttct
     1861 tcttctcgat atttcaagtt ttaggaatga aatcgaaacg cctggtcgat ttttggactc
     1921 tgagttcctt aattggattt ctccgacaga atggatagag agctattctc tgaaaatgaa
     1981 gaaccagtca gctcatctag atagcactga agaagcacaa tctcaaccag agcttagtaa
     2041 agtcgagcag cctcgacaag agaccaacaa attggctcag cccgaaaaag aattctctct
     2101 ggaaccttgc agcgccacac aaaaggccac acaaaagcga tctatgcttt ttcccgaaag
     2161 cttcgaaaca agtcgaatcc cctatgctta tggcacattg ctcggtatag gattcatagc
     2221 gatggatctt accgaacggc tatcctggtc agcttttgtt ggatgggatt ggctaatagc
     2281 aagaccagct tcaagcatcg acttaagttt gagtgccgga gcttggagct tcgagatctg
     2341 tgcgtgggat caagggatct ttgcttttcc tgttgcgcgc atatctgccc ttgagggggc
     2401 gctggactgg gaacataccc ttgtgcccac atttagaagc atttctgcag ggatccatgc
     2461 tcgatacgga gctgtgcaaa ataaatatga agagcaaggc ttgtttcatc aatcaccgga
     2521 ctatttcgca tgggtagcgg ggaccggagt cgagttcgat agtctttctt cttcttatag
     2581 accaccatac gatccttccg gtatttcgct caagggcaga atcgaatatg aaggcgctaa
     2641 ttcactgaaa tttggtggtg tttctttatc aggatcgatc tatctgggag aatcgacaat
     2701 tgcaggacga atcggtgctt ccatctatgg agcctgggct cccaccggag gagtagcttt
     2761 ttcgccagca actcgatatc tggaatatgc tggagaccat gagttgtcgg tagcagatct
     2821 tccatatcct gcatataagg aatatcaggc ttgctcagaa ctctctgcct ggtatctata
     2881 tggagagata aaggccaggc tcttttcttt agagctaaac tggattatga gcaatctctt
     2941 tgctccttct ttagcattga gaagaatatc gatgatttca ggattgcgcg gtgcgggcct
     3001 tgagatcgac aatgcacctt accttttgag ttcagctttt ctccaagcca agttcgattg
     3061 tgcttttctc tcaggcgttg tggccgcaac tcacatgaaa ttcactttgg aggcggcttg
     3121 ggcttttcaa gctgataagg caggtggaga acccttctgc ttttctgtag ggctgagtgg
     3181 gcaattttag tttgcttcaa actatctcga tagagagtat agaaagaatc ttgcggatca
     3241 tcgcagagaa tcagtgtgct tttcatgaaa tgtgtcccat tgcacaagaa ggcttcctta
     3301 tgccattacc aattcttata aacaaggttc gtagcagtat gttccttgat aactacacat
     3361 ctgtatagta taattcaaat atggagacaa gcttttcccc accactcgat ccttgtacct
     3421 atgatctcag aattgggaca gtggggtatg aatattctga atgggagggg gtgctgtatc
     3481 ctaacggaat agaaaaagaa acatatttgc gcttatattg cgagcgcttt tctactctcg
     3541 agctggattt tacattccat accatgccca aggccgccca aatgcgagat attc
//
LOCUS       DAOMZL010000129         3582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_25035_length_3582_cov_13.791608, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000129 DAOMZL010000000
VERSION     DAOMZL010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25035_length_3582_cov_13.791608"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PLQ30_07920"
     CDS             <1..437
                     /locus_tag="PLQ30_07920"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW02414.1"
                     /translation="VTIRLVFDLLVAVIAVVVVMIMMNVLVISVNERLHEIGTLRAIG
                     AKRHVVRSMILYETTFMVLIAGAVGLILGYILLLIMGKVGIRAPNLFFEALFGGEILR
                     PLISIGAAGRAFFWVLAMALVASWYPTRVAVRIEPVVAMRGD"
     gene            440..1747
                     /locus_tag="PLQ30_07925"
     CDS             440..1747
                     /locus_tag="PLQ30_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW02415.1"
                     /translation="MHFSNFKLHLKLAARNLRRQPNRTIALGGAVAFSALVMTLISGF
                     VGGMDKAIQDNVTLYSGGHILISGYTPSWSGRLQNRFIDDEIAKTVESAIPDVRTVSP
                     LAQARATVVFGTREIQLTLRGVDWTKDELYHDSLILSSGDWEALNEQDRSMLMSSQTA
                     DRFGLAIGDQVVVRLTTASGQQNVTDYAVSAIYDDAASGGMTTAFVSFGDLIADLNMK
                     ANEQQQIAIFLKDSTDAERAAKAIAEALTEKGYSIASGTSGSEQASQRRMNSEKAVYS
                     ISTVQELAGEIGSALGSIRWIGYAVFILMLLVSATGIANSYQMVLLERTKEIGMLRCI
                     GFKKKDVFASFIFEGILLAGAAAFAGIICALPIGLGISFIPFDPHGDFGAALVKGHLR
                     FAPTLDQLLIILVFIMIVAIVAVFLPARKAAEVVPVEALRITA"
     gene            1789..2706
                     /locus_tag="PLQ30_07930"
     CDS             1789..2706
                     /locus_tag="PLQ30_07930"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPW02416.1"
                     /translation="MLRFYEKRTWFFSSKSIRDISLVLLGFVIVMCAQAQGGPPPGGP
                     KGGGGPMAGIGLPLGFLEADKDYKFPDDITKYTANDFLMLVRRTDIRSSFYNSDMNAT
                     ITTVNVSPTRGTFVTKEQIFRRDKDDAFLMLTLEPESRKGQGMLRVDNNMWRYDPTSR
                     KFTHTTLKDTYENTTVTNNDFRRWQRSIDYSVEKLSVGTLGNYKVIIGELKANNDEVP
                     FPYIKMYIEADRKIVLKVEEYSLSMKLLRTAYYTQYVQIGDSFVPTVQIFQDGLVPEK
                     RSQVTYTNISTKSLPDYYFTKEYLERVSQ"
     gene            2718..>3582
                     /locus_tag="PLQ30_07935"
     CDS             2718..>3582
                     /locus_tag="PLQ30_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02417.1"
                     /translation="MKTRILKGLIFLIVMTLFLISTSGIWAQASGTGTSLIPPEVIDE
                     NELFGGPKDIVTVIDPSVASAGTVQLVQDTKAYPVFLIGGSAGAGLYGSYTPLGSSSS
                     DTQTLYGAVDISDLEFDYLPAENLHFSISSNLLAVPNQILAASASAYADLRQSDLVRL
                     YVSGSFNYDPSSYLSTNEEYTSPTFSLDELFVDTHIEETVFFRLGKQRISWGVGNWFK
                     PSDILSLSAIDPDDPTAAREGPFAFKVDVPMKLNHLMLYMVPPVSDDAASSSIASKYD
                     LVVNGWELSFGA"
BASE COUNT          928 a          787 c          883 g          984 t
ORIGIN      
        1 cggtaacgat tcggcttgta ttcgatctat tggttgcggt cattgcagtc gtcgtcgtca
       61 tgataatgat gaatgtgctt gtcatatccg taaatgagcg cctccacgag attggcacac
      121 tcagagccat aggagccaaa cggcatgttg ttcgcagcat gattctatat gagaccacat
      181 tcatggtgct tatcgctggg gcagttggtc taattctagg gtatatcttg ctccttatta
      241 tgggaaaagt cggcattaga gcgccaaatc tctttttcga agctcttttt gggggagaaa
      301 tcctaagacc gcttatatct atcggagcgg ctggtcgtgc gttcttttgg gttctagcca
      361 tggcattggt tgcttcctgg tatccaacaa gggtggcagt gcgaatcgaa ccggttgtcg
      421 caatgagggg agactgaata tgcatttttc aaattttaaa ctccatttga aacttgcggc
      481 tcggaacctt cgtaggcagc ccaatagaac gattgcgctc ggaggagcgg tggcttttag
      541 tgcactcgtt atgactctga tttcagggtt tgtgggcgga atggataagg caattcaaga
      601 caatgttacc ctttattccg gcgggcacat attgatttcg ggctatacac ctagctggag
      661 cggtaggctt caaaatcgat ttatagatga tgagatagcc aaaactgtag aatccgctat
      721 ccctgatgtc aggacggttt ctcctcttgc tcaggcaaga gccacggtag tctttggaac
      781 tagagaaata cagcttacgc tccgtggtgt cgactggaca aaagatgagc tatatcacga
      841 ttccctcatt ctatcgagcg gagattggga agcgctgaat gagcaggata gaagcatgct
      901 catgagctct cagacagcag atcgattcgg ccttgcaatc ggggatcagg tggtcgtgag
      961 gctcacaacg gcatccggtc agcaaaatgt tacagattat gccgtttcgg cgatctatga
     1021 cgatgccgca tcggggggaa tgacaactgc ttttgtttct tttggggatc taatagcaga
     1081 tctgaatatg aaagcaaatg agcagcagca gatagcgatt tttctaaagg acagcaccga
     1141 tgcagaacgc gcggccaaag ccattgccga agcgcttacc gaaaaaggat atagcattgc
     1201 ctctggaacc tccggaagtg agcaggcaag tcaacgccgg atgaattccg aaaaggctgt
     1261 ctattcgatt tccacagtac aggagctcgc tggggaaata ggctccgctc tcggctccat
     1321 tcgttggatt ggatacgcag tgttcattct tatgctgctt gtaagcgcta ctggcattgc
     1381 caattcatat cagatggtgc tcctcgagcg gactaaggaa ataggtatgc tacgatgcat
     1441 tggttttaag aagaaagatg tatttgcatc atttattttc gaaggaatct tgcttgctgg
     1501 agcagcagcg tttgcaggaa ttatatgcgc cctgccaata ggcctgggta ttagttttat
     1561 tccctttgat ccacatggag actttggagc agccctggta aaaggacatt tgcgatttgc
     1621 tccaaccttg gatcagcttc taataatctt ggtgtttatc atgattgtgg ctattgtcgc
     1681 agtctttttg cctgcccgaa aggcggctga agtagtgccc gtcgaggcac ttaggatcac
     1741 tgcgtaatgc ttatgatatt gcgattgcgc agaaactagg agcgtttgat gttacgattc
     1801 tacgagaaaa ggacttggtt tttctcatca aagagcatca gagatataag ccttgtactc
     1861 cttgggtttg ttattgtcat gtgtgcgcaa gctcaaggtg gcccgcctcc gggcggacca
     1921 aaaggtggcg gaggacctat ggctggcatt gggctcccgc tgggctttct ggaagcagat
     1981 aaggactaca aatttcctga tgatataaca aaatatactg caaatgactt tctaatgctc
     2041 gttcgccgga cagatatccg ttctagcttt tataacagcg atatgaacgc tactatcact
     2101 acggtaaatg tgagcccgac gcgcggtacc tttgtaacca aggaacaaat cttcaggcgg
     2161 gacaaagatg atgcattctt gatgctcacc ctggagccgg aatcccgcaa gggacaagga
     2221 atgctgcgcg ttgataataa tatgtggcgc tacgatccaa caagccgaaa attcactcac
     2281 acaaccctca aagacactta tgagaatacg acagttacta ataatgattt tcgtcgctgg
     2341 cagcgctcga tagattattc tgtggaaaag ctctctgtgg gcactcttgg aaactacaag
     2401 gtaataatcg gagaactcaa ggccaacaac gatgaagttc catttcctta tatcaagatg
     2461 tatatagaag cggaccgaaa gatagtgttg aaagtagaag aatatagcct atccatgaag
     2521 ctcttgcgca cagcttatta tacccaatat gtacagatag gagattcctt tgtgcctacg
     2581 gtgcagattt tccaggatgg ccttgtgccc gagaagcgct ctcaggttac ctataccaat
     2641 atctccacaa aatcgctccc tgattattac tttaccaaag aatatctcga acgggtgagt
     2701 cagtgaggag cagaggcatg aaaacaagaa tactaaaggg cttgatattt ctgatagtta
     2761 tgactttatt tcttattagc acatccggta tctgggctca agcctctggc acaggaacta
     2821 gcttgattcc tcctgaagtc atagatgaaa atgagctatt tggcggccct aaggatattg
     2881 tgacggtaat agatccgagc gtagcttcgg cgggaaccgt gcaacttgtc caagatacca
     2941 aggcttaccc ggttttcttg atcggcggat cggctggagc tggtttatat ggtagttata
     3001 ctccccttgg gagcagctcc tcagatacac agacgctcta tggggcggta gatatttctg
     3061 atcttgaatt cgactatctt cctgctgaga acctgcattt ttccatatcc agcaatcttc
     3121 ttgcagtacc aaaccaaatt cttgctgcct ctgccagtgc atacgcggat ttgcgtcaga
     3181 gcgatctcgt gcgactctat gtctcaggct cattcaacta cgacccatcg tcctatttat
     3241 ctaccaacga agaatatacc tctcctacct ttagtctaga tgaacttttt gtagatacac
     3301 atatagaaga gacggttttc tttcgtctag gtaagcagcg cattagttgg ggagtaggaa
     3361 actggttcaa accctctgat attctgagcc tttcggctat tgatccagac gacccaacag
     3421 cagcgcgcga gggacctttt gctttcaaag tcgacgtacc gatgaaatta aaccacctga
     3481 tgttatatat ggtaccacca gtttcagatg atgcagcttc gtcttcaatt gcgagcaagt
     3541 acgacctcgt agttaatgga tgggagctca gttttggtgc ct
//
LOCUS       DAOMZL010000130         3510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_25763_length_3510_cov_11.386107, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000130 DAOMZL010000000
VERSION     DAOMZL010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25763_length_3510_cov_11.386107"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..677
                     /locus_tag="PLQ30_07940"
     CDS             213..677
                     /locus_tag="PLQ30_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02418.1"
                     /translation="MKKKVFLLVLAALLISVPVFAATQVETTIQATIGADLSITTTIP
                     GTKAIDIAASSTTLGSVTISSNLTNWKIRIHSGNGGKMIRVGGADVYPYKMNFGTISD
                     IDLATDKVIDKTAPQSATTTSVVVTYTKAAEMIPALPAGTYEDTITISLSAT"
     gene            757..2406
                     /locus_tag="PLQ30_07945"
     CDS             757..2406
                     /locus_tag="PLQ30_07945"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW02419.1"
                     /translation="MSAISSQKKNKAKVATVLLSFLFCTLAIFFFVMLWKTANRSESE
                     LLETVSKRIALQISAPLQEMLTVGEDFRELLIADSGKTYAALRPLAEEALSHLPYIDS
                     ITIAPGAIVRYFFPEGSASSSIGHDLLDNPERMHALVEAVRRKSAVLQGPELSAEGKN
                     LAFLRIPVIEGSELWGFVSIAFNADKVLSDLDLVSQFPGLSIAIVSSDMEGNGRKVFW
                     GEERALSGYSAVLKIGSEDLPWTIYVASMYPYRQVAIWGAGLLILVLLSSGLIIRAGS
                     LKNESKVPVWPKEASMAIKPFVPKTESIKEPSINVVNKVEIPQASAIEESMAQESAAE
                     KPVSPSLKKPTKEPAGESKMDMAVLQNQKIENENRSDEVAVPKEEIPSSISVLVVDDS
                     EVNRDLLLRMLTLKDYKAKAVSSGKAALQALSENIFDVLLVDCIMPEMDGYALAKEIH
                     AKETSKVASSLQERNYLKYKPIAQSSNAENGISECGILKRSISQSDVSRPILIAMSPR
                     HDQEEAEKCANAGFDSLLVKPFTMTALDQQIRLAIGNRSAE"
     gene            2773..3252
                     /locus_tag="PLQ30_07950"
     CDS             2773..3252
                     /locus_tag="PLQ30_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02420.1"
                     /translation="MKKYALVALFAVVSLGMVFAVVANPGSGTVSIQGSVDQYFSLTV
                     PASYTGSISYDSSTHWDLSDMKVISNVKNWVLKASSQNNGVLKDTANGADIAYKVTVG
                     SLFTKQQLTSGGITSGPQSKTPKAGTAYKFGIDIDAPSELYQAGTYSDVITLTISNQ"
     gene            complement(3320..>3510)
                     /locus_tag="PLQ30_07955"
     CDS             complement(3320..>3510)
                     /locus_tag="PLQ30_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02421.1"
                     /translation="KMITLAAIKYNVALKYLNLKGRRSKEFRFISKKYRKTGKGTRTQ
                     ATNSFSMAKSINIDKKQG"
BASE COUNT         1020 a          702 c          831 g          957 t
ORIGIN      
        1 agggagaggt ctgcaaaacc tttatacatc ggttcgattc cgatcggcgc ctttactaaa
       61 aatatcataa gaaaattggc tattggactc ttgacaatta ttcagcaaaa gcaatgggtg
      121 cacgacaatg ggagtcttga ctggaatata agtcatggta taaccttccc ttaaattatt
      181 taagggctct ttttgagcca aggagaaagt ctatgaaaaa gaaagtattt ctgttggtac
      241 tggcagcatt gttgatcagc gtgccagtgt tcgccgcgac tcaggttgaa acaacaattc
      301 aggcaacaat tggagctgac ttaagcatca cgaccactat tcctggtaca aaagccatcg
      361 atatcgccgc tagctcgacc acgctcggtt cagtcacgat ttcgtcgaat ctgacgaact
      421 ggaagatccg tattcattca ggcaatggtg gcaaaatgat cagagtaggc ggtgcagatg
      481 tctaccccta taagatgaat ttcggtacta tttcggatat cgatcttgct accgacaaag
      541 taatagataa aacagcccct cagtctgcga caaccacaag tgtcgtggta acctatacga
      601 aagctgcgga aatgattcct gctcttcctg caggtactta tgaggatacg ataacaattt
      661 cactttctgc aacttgaaag aattattcaa tagagtgata aaaggctgcc cgcttaatgg
      721 gcggcctttt ttatgatatt cttatatgat cgaattatga gcgctataag cagccaaaaa
      781 aagaataaag ccaaggtggc gacagtcttg ctttcattcc tcttttgcac gctagcaata
      841 ttctttttcg taatgttatg gaagaccgca aacagatcgg aatcagaact gcttgaaact
      901 gtctccaaga gaatagcgct gcaaatatca gcgcctttgc aagagatgct aactgttgga
      961 gaggactttc gtgagctcct aattgctgat tcaggcaaga cctatgcggc gctcagacca
     1021 cttgccgaag aagccctttc tcacttgcct tatatagact ctattacgat agctccaggt
     1081 gcgatcgtta gatatttctt tcccgagggt agtgcttctt cttcgattgg ccacgatttg
     1141 ctcgataatc ctgagagaat gcatgctctg gtcgaggcgg tgcgtagaaa gagcgctgtc
     1201 ttgcaggggc cagaactatc cgcggaagga aagaatctcg cgttcctgcg aataccagtg
     1261 attgagggct cagaactctg gggttttgtg agcatcgcat tcaatgcgga caaggtattg
     1321 tcggatctag atctagtttc gcaattccca gggctatcca ttgccattgt gtcttctgat
     1381 atggaaggga atggcagaaa ggttttttgg ggcgaagagc gtgctctctc cggttattcg
     1441 gctgttctga aaattggatc ggaagatttg ccatggacta tatatgtcgc atcgatgtac
     1501 ccatataggc aagtagcgat atggggagcg ggactcctta tacttgtgct attgagctct
     1561 gggctcatca tacgagctgg ctctttgaag aatgagagca aagttcctgt gtggcctaaa
     1621 gaagcttcaa tggccataaa gcccttcgtc ccaaaaactg aatccataaa agagccttca
     1681 ataaatgtag tcaataaggt cgaaattcct caagcatcag cgatagaaga gtcgatggca
     1741 caagagtcag cggcagaaaa gccggtcagt ccttcgctaa aaaagccaac aaaggagcca
     1801 gctggtgaat ctaaaatgga tatggctgtc ctgcaaaatc aaaagattga aaatgagaat
     1861 agaagcgatg aagtcgctgt gcccaaagaa gagattccaa gttccatatc ggtgctcgtc
     1921 gtcgacgata gcgaagtgaa tcgcgatctt cttttgcgaa tgctcacatt gaaggattac
     1981 aaagcaaaag cagtttcgag tggaaaggct gctctacaag cgctaagcga aaatatcttc
     2041 gatgtcttgc ttgtagattg tatcatgcct gagatggatg gttatgcgct tgcaaaggaa
     2101 atccacgcaa aagagacctc gaaagtggcg tctagtctac aggagcgcaa ttatttaaaa
     2161 tataagccca ttgcacagag cagtaatgcg gaaaatggca tttcggaatg cggtatcctg
     2221 aaaagaagta tctcgcaaag cgatgtctca aggcctatac tcatcgcaat gagtccgcgt
     2281 catgatcagg aagaagctga gaaatgtgct aatgctggct tcgacagtct cctcgtaaag
     2341 ccttttacga tgacagccct tgaccagcaa attcgcttgg ctattggcaa tagaagcgcg
     2401 gaatgagggc ttgcttacta agggtctgta taggcatcaa aaatagcaca gccctagtgt
     2461 cgacattgtg aaataataat ttagtttata gagatattag acataagtgg caaaattctc
     2521 tattttatcg aatagcttta atttacagtg gatgcattag atagctaatt tattgcttaa
     2581 taagaatata tcagtactgg ttgaaagagt ccaagaatct taagtcatga aagatagaga
     2641 aaaactcatt gtacaagaaa cttatgacga ttataataat atcaaagtgt agtggtggaa
     2701 tccgttggag gaaactacaa aaagcacaaa atgtatccgt ggggcataga ccctgcgggc
     2761 gggagacgca aaatgaaaaa gtatgcgttg gtagctctgt ttgcggtagt gtcgttaggg
     2821 atggtgtttg ctgtagtagc aaatccaggt agtggtactg tatcgatcca aggcagtgtc
     2881 gaccagtatt tctcgctgac tgtgcctgcg agctatacgg gtagtatctc gtatgacagt
     2941 tcaacccatt gggatttatc tgatatgaaa gtgatttcca atgtgaagaa ttgggtactt
     3001 aaggccagca gtcagaataa tggtgtttta aaagacacgg ccaatggtgc ggatatcgct
     3061 tataaagtta ctgttggtag tttatttact aaacaacagc taacaagcgg gggtatcacg
     3121 tctggtccac agtcgaagac accaaaagcg ggaacagctt ataagtttgg tatcgacatt
     3181 gatgccccga gcgaactcta tcaggctggt acctacagcg atgtaattac cttgacgatc
     3241 agcaatcagt aatccgtaac agagtgatta caggaaggcc gtccggacca aaaccgggcg
     3301 gcctttcatt tttaagtagc tatccttgct ttttgtctat gtttatactt ttagccatgc
     3361 tgaaagagtt ggtggcctga gtccttgttc cctttcctgt ttttctatac tttttactta
     3421 tgaatcgaaa ttctttgctg cgtcgtcctt tcaaattcaa atacttcaat gcgacattgt
     3481 atttgatcgc cgctaatgtg atcatctttg
//
LOCUS       DAOMZL010000131         3461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_26277_length_3461_cov_20.524662, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000131 DAOMZL010000000
VERSION     DAOMZL010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26277_length_3461_cov_20.524662"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2023
                     /locus_tag="PLQ30_07960"
     CDS             <1..2023
                     /locus_tag="PLQ30_07960"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPW02422.1"
                     /translation="TASASIWQCGNGLIYSMKNAALILSISKVFNFLGIIMIAPGIYF
                     FTITSLGLFEQKKRYILANYAIALVFYILGLLTDWLVASVEGHFYGYEIVFGPLAIPF
                     LLFFSVLIIRSLQLDRRAIRQMEPGIKRDQIKLFMISLSIAALASVDFLSSIPSLHFY
                     PIGFLPVLIFISLQAYAIIKYKRASLGTIFEAIEDGIIVIDKNDKIEEVNPSMETMLG
                     IKRHDLLGKDFLSIFSLVSSGKEDPEKAEATIKYLLLTPTKVADEDIAFKEPEQQLNV
                     VSSPILDRFGVRKGNVLIFRDITERKKMEEELRKYKESLEELVAARTEQLKKSEGKYK
                     ALVDHALVGIGIHQNTQMVFVNRQFASMLRFSEEEMIGLPISQLIHPDERELIMSRAY
                     DRYSGKEVIETYEMRMVRKDGSFMPAFISNTAIEYNGARATLITVVDTTETKLRKELE
                     QVNEELERFTYSISHDLRAPLRSIDGFSLALQEDYEDRLDREGQDYLQRIRAACQRMA
                     AMIDAILQLSRIGRYEMHREKVNLSALAQDIALELEATSPDHKVEFSITEGIVAAGDP
                     MLLRMALENLIGNAWKFTKERECARIEFGVMQQNFKTIYYVRDNGTGFDMKYVDKIFD
                     PFQRLDNAKAFSGTGIGLASVKRIVHRHGGEIWAESAFDKGATFYFTLE"
     gene            2056..3432
                     /locus_tag="PLQ30_07965"
     CDS             2056..3432
                     /locus_tag="PLQ30_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPW02423.1"
                     /translation="MDKPLRVLLIEDSEDDALLILRELRKGGYTPVYERVETEFVMRS
                     ALSSQQWDVILSDYNMPEFCAGEALSVFRECGIEIPFIIVSGAVGEEKAVQLMKEGAH
                     DYVMKDNLQRLVPAIERELKESEDRKSRKIAEDSYRKSDFIVNASRDMMALINRSGIF
                     ETVNSSFCKIFGKERQQEIVGHSVSEIWGQEVYEQKIKPCLERCQNGEDINTEDWFDM
                     EGMGTQCFEINYIPYRNSDGVVTHAIMAAHNITNRKMAEQELDKSYRNLQKTLEETVN
                     ALSALVEMRDPYTAGHQNRVAGIARAIAQELGLSEDAAQGIWVASLIHDIGKVRVPAD
                     ILSRPAHLSSAEFELIKEHPRTGYEILREIDFPWPVAEIVLQHHERINGSGYPQGLKG
                     DGILLASRIIGVADVVEAMTYHRPYREALGLDAALDEIRENKGILYDPDVVEACIRVF
                     MEKGLALD"
BASE COUNT         1041 a          671 c          820 g          929 t
ORIGIN      
        1 gacagcaagt gccagtattt ggcaatgtgg taatggctta atctattcaa tgaagaatgc
       61 agcactgata ctgtctattt ccaaagtatt caatttttta ggcatcatca tgattgcccc
      121 tgggatatac ttttttacca taacatcact gggcttattc gagcaaaaga agcgctatat
      181 tcttgcaaat tatgccattg ctctagtttt ctatatatta ggcctgctga cagattggct
      241 cgtcgcttct gtagaaggac acttttatgg ctatgaaata gtatttggtc cattagcaat
      301 acctttcctg cttttctttt ctgttctcat aattcgatct ctacagctcg atcgaagagc
      361 cattagacaa atggagcctg ggataaaacg ggaccagata aagctgttca tgatatcctt
      421 atcgatagcc gcattggctt cggtcgactt tctatcctcc attccatcgc ttcatttcta
      481 tccgattggt tttctgccag tccttatatt tatctcgctt caggcttatg caataattaa
      541 gtataaaaga gcttctctgg gtactatttt tgaagcaatc gaagatggga tcatcgttat
      601 agataagaat gacaaaatag aagaagttaa cccatccatg gaaaccatgc tgggcatcaa
      661 gaggcacgat cttcttggta aagattttct tagtatattt tctctagttt cgagcggtaa
      721 agaggatcct gaaaaagcgg aagctacgat aaaatacttg ttgctcacgc ctacaaaggt
      781 cgctgatgaa gacatagcat tcaaggaacc tgaacagcaa ctaaacgttg tatcttcgcc
      841 gattctggat cgctttggtg tgagaaaagg taatgtactt atttttcggg atataacaga
      901 aagaaaaaaa atggaagagg aattaagaaa gtataaggaa tcgctcgaag aactagttgc
      961 agctcggaca gaacagctca agaaatcgga aggaaaatac aaagcacttg tggatcatgc
     1021 attggtcggc attggcattc atcagaacac ccagatggtc tttgtgaata ggcagtttgc
     1081 atctatgttg aggttctctg aagaagagat gataggtctg cccatctctc aactcattca
     1141 tccagacgaa agagaactta ttatgtccag agcctatgat agatatagtg gcaaagaagt
     1201 tatcgagact tatgagatgc gaatggtcag aaaagacggc agtttcatgc ctgccttcat
     1261 tagcaatacg gcaatcgaat acaacggggc aagagcaacg ctcatcaccg ttgtcgatac
     1321 aacagaaacg aagcttcgaa aagagctgga gcaggtaaac gaggaactcg agagattcac
     1381 atactccatc tcccatgatt tacgtgcgcc gcttaggagc attgatggtt tcagtctagc
     1441 tctgcaagaa gattacgagg atcggctcga tcgagaagga caggattatc ttcagcgcat
     1501 aagggcagca tgtcaacgaa tggcagccat gatagatgca attcttcagc tctctcgcat
     1561 tggaagatat gaaatgcaca gagaaaaagt aaatcttagc gcgttagcac aggatatagc
     1621 cctagaactt gaagcaacct ctcccgatca caaggtggaa ttttcgatca cagaaggcat
     1681 cgttgcggcc ggagatccaa tgcttctaag gatggctttg gagaacctga ttggcaatgc
     1741 atggaaattc acaaaagaac gtgaatgcgc aagaattgaa tttggcgtca tgcagcagaa
     1801 tttcaagacg atttattatg ttcgcgacaa tgggactggg ttcgatatga aatatgttga
     1861 taaaattttc gatccatttc agcgtctcga taatgcgaaa gcttttagtg gaacaggcat
     1921 agggcttgct tcggtaaagc gaatcgttca tcgccatggt ggagaaatct gggctgaaag
     1981 cgcctttgat aaaggcgcta ccttctattt tacattggaa taattattat tttagatcac
     2041 tatatggaga accgaatgga taagccattg cgagtgctgt tgattgagga ttctgaagat
     2101 gatgcccttc tgattctgcg cgaattgcgc aaaggtggtt atacgccagt atatgagcgc
     2161 gtggaaaccg aatttgtaat gagatcggct ctatcatctc agcaatggga tgttatcctt
     2221 tcggattata acatgccaga attctgcgct ggcgaagccc tatctgtttt tagagaatgc
     2281 ggaatagaaa taccttttat cattgtttct ggagctgttg gggaagaaaa agcggttcaa
     2341 ctcatgaagg aaggcgcgca tgactatgtg atgaaagaca atctccagcg tcttgttcct
     2401 gcaattgagc gagagctcaa ggaatctgag gatagaaaat cccgaaaaat cgccgaggat
     2461 tcctatagaa aatccgattt tatcgtcaat gcttcgcgcg atatgatggc tttgataaat
     2521 agatctggaa tatttgaaac tgtcaatagc agcttttgca agatttttgg caaagaaagg
     2581 cagcaagaaa ttgtaggcca ttcagtaagt gaaatttggg gccaagaagt ctatgagcaa
     2641 aaaatcaagc cttgcctcga acgctgtcaa aatggagaag atatcaatac cgaagactgg
     2701 ttcgatatgg aaggaatggg cactcaatgt ttcgaaatca actatatccc ttataggaac
     2761 agcgatggag tcgttacgca tgccataatg gcggctcata atatcacaaa tcgaaaaatg
     2821 gcagaacaag agctggataa gagctatcgg aatctgcaaa aaacattaga ggagaccgtt
     2881 aatgcgcttt ctgccttggt agaaatgcga gatccgtata cggcagggca ccagaaccgg
     2941 gtcgctggga ttgccagagc tattgcccag gagcttggct tgtcagaaga tgctgcccaa
     3001 ggcatatggg tagcttcgct gatacatgat attggcaaag ttcgtgtgcc agccgacatc
     3061 ctttctagac cagctcatct ttcaagtgca gaattcgagc ttatcaaaga gcatcctcga
     3121 acaggttatg aaatactaag agagatagat ttcccatggc ctgttgctga gattgtgctc
     3181 caacaccatg agaggataaa tggatcaggc tatccgcaag ggctcaaagg ggatggcatc
     3241 ttgcttgcaa gcagaatcat aggcgttgcc gatgtcgtcg aagctatgac ttaccaccgc
     3301 ccgtatagag aagcacttgg actggatgca gcgctcgatg agattaggga aaacaagggg
     3361 atcctgtatg atcccgacgt agtggaagct tgcattaggg tttttatgga aaaaggtttg
     3421 gcactcgatt gatttctatt caccatgata tttatgcatg c
//
LOCUS       DAOMZL010000132         3396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_26946_length_3396_cov_16.140078, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000132 DAOMZL010000000
VERSION     DAOMZL010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26946_length_3396_cov_16.140078"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1652
                     /gene="secD"
                     /locus_tag="PLQ30_07970"
     CDS             <1..1652
                     /gene="secD"
                     /locus_tag="PLQ30_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPW02424.1"
                     /translation="LTPKEDQAIAVGSREQIREYSRRMTYLIISDLKQRAATGDTTDL
                     SNQKNYKVAIDSAKRAYSLAKKPAPRTWDAVAILSAFSSERSMFDTIESRYREYVLKL
                     KNVHSNAVQLGLDLAGGMSAVVQADLKGLSEKLGHELSQAEKEDAMKRAVEILNSRID
                     KFGLTEPVIRRQGEDQIYIEIPGTPDPERINSIIMGKGNLAFYIADLEASAAVNSYLS
                     TNPVGIDEETMTVDKPGLVPEGKIVRKVYKKDSYGLDEFTGEYLVLEGKPGLDGSHIV
                     SATVSSDPITGQPETNFVLDKEGGDIFYELTSANVGKTMAVVLDDRVKSYARIQEPIR
                     ESVRITGFSAEEAEALALLLRTAALPISLSVVSQQSIGASLGEDAISQGQNAIIVGIL
                     SIFVFMFIYYKWAGINATIAQILNVYLMLSVLTAFKLTLTLPSIAGFVLTTGMAVDAN
                     VIIFERIKEEMKAGKSRAAAIHAGFDKAFWAVMDSNITTIIAALFLAQLGTGPIQGFA
                     VSLAIGNITSLFTSLFVSRLIFDFETDVLKLKTVSISWRIR"
     gene            1652..2902
                     /gene="secF"
                     /locus_tag="PLQ30_07975"
     CDS             1652..2902
                     /gene="secF"
                     /locus_tag="PLQ30_07975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPW02425.1"
                     /translation="MKSIIKFSKFFLPAIIISLSIIAFGLAGFFTKGLNLGVDFQAGI
                     NETIQLAYPAGQVVFEGKGNAELTIGESQLTLVFSGAEAQKRTVLFDYKTYSTLADIK
                     AALEKEQGIRVDLAEGSDSLPSNLLVPTYQGNSLLSLNPVKLYRAPKDDSELYASIDI
                     VRDALSPLGDITVQTINPPSSQRFLIRVRDNGEDPQFMTNVPQTIRNLIEDKVGADRL
                     VVVKADYVGPQYSETLSRQSIWLVLMTLVLILVYSTIRFKIEYAIAAVAAIAHDALVM
                     IAFIVWTRMEFNTTTIAAILTILGYSINDTIVQFDRVREERKLHPSEPFVDVLDRALT
                     LTLGRSIITTLTTMLAVLALYFFTSGSIKDFALALLVGMVSGVYSTIFIASAIVLFLE
                     NKKTKRRAKTIKEKTAPAGALSKQ"
     gene            2998..3069
                     /locus_tag="PLQ30_07980"
     tRNA            2998..3069
                     /locus_tag="PLQ30_07980"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3030..3032,aa:Cys,seq:gca)
BASE COUNT          936 a          766 c          816 g          878 t
ORIGIN      
        1 tcctgacacc gaaagaggat caagccattg cggtaggttc cagagagcaa ataagggaat
       61 attcacgtag aatgacctat ctcataatca gcgacttaaa gcaaagagcc gctacaggcg
      121 atactacgga tctctctaac cagaagaact acaaggttgc aatagattcg gcaaaaaggg
      181 cttattcttt agccaagaaa ccagcaccca ggacatggga tgcggtagcc attttatcgg
      241 ctttttccag cgagcgctcc atgttcgata cgatcgaatc tcgctataga gaatacgtgt
      301 tgaaactcaa gaatgtgcac tcgaatgcgg tgcagcttgg actcgacctt gccggcggta
      361 tgagtgcagt tgtccaggct gatctcaagg gattgtcgga gaaactaggg catgagctat
      421 cccaggccga aaaagaagac gccatgaagc gcgcggtgga aatcctcaat tcgcgcatcg
      481 ataaatttgg ccttacagaa ccagtcattc gtcgccaggg tgaagatcag atctatattg
      541 agattccagg aactccggat cccgagcgca taaattcgat tataatgggt aaagggaacc
      601 tagccttcta tattgcggat ttggaagcct ctgcggccgt gaattcctat ctttctacta
      661 atcccgtagg catcgacgaa gagactatga cagtggataa gccaggcctc gttcccgaag
      721 gcaaaatcgt ccgaaaggtc tacaagaaag acagctatgg tctcgatgaa ttcaccggcg
      781 aatatcttgt tctcgaaggt aagcctggtc tggatggcag ccatatcgta agcgcaacag
      841 tctcttccga tccgataaca gggcagcctg agacaaactt tgtgctcgac aaagagggcg
      901 gagacatatt ctacgagctt acaagtgcta atgtcggcaa gactatggcc gttgtgctgg
      961 atgaccgcgt aaaatcctat gctcgcatcc aggaaccaat tagagaatcg gtacgaataa
     1021 cgggattcag tgccgaggaa gccgaagcac tggcgctatt gctgagaacg gccgccctgc
     1081 ccatcagcct ttctgtggta agccagcaat ctatcggcgc ttctcttgga gaagacgcca
     1141 ttagtcaggg acaaaacgcc ataattgtgg gtattttatc gatcttcgtt ttcatgttta
     1201 tttattataa atgggctgga attaatgcga cgatagcaca gatactcaat gtatacctca
     1261 tgctctcggt cttgacagcc ttcaagctga ctctcaccct tccttcgata gcgggttttg
     1321 tgctcactac tggaatggca gtagatgcca atgtcatcat cttcgaacgc atcaaggaag
     1381 agatgaaagc aggcaagagt agagcggcgg ctatacatgc aggtttcgac aaagccttct
     1441 gggcagtcat ggactcgaac ataacgacga taattgcggc acttttcttg gctcagcttg
     1501 gaaccggccc aattcagggt tttgcggttt ccttggcaat aggtaacata acgagtcttt
     1561 ttacctccct tttcgtgtcg aggctgattt ttgatttcga aactgatgtg ctaaagctca
     1621 agacagtgtc cataagctgg aggatccgat aatgaaaagc ataattaaat tcagcaaatt
     1681 ctttttgccc gcgataatta taagtctatc cattatagct tttgggcttg ccggattctt
     1741 cactaaaggc ttgaacctgg gcgtggactt tcaggcagga ataaatgaga cgattcagct
     1801 tgcctatcca gccggacaag tcgttttcga aggtaagggt aatgctgaac tgactatcgg
     1861 agagagccag ctaacgcttg tcttctcagg cgcagaagcg cagaagcgta ctgtgctgtt
     1921 cgattacaag acatactcga cccttgccga cataaaagct gcactggaaa aagagcaggg
     1981 aatcagagtt gaccttgcag aaggaagcga ttctcttcct tctaatcttc ttgtgcctac
     2041 ttaccagggc aattctctgt tgagcctaaa tcctgtaaaa ctctaccgcg cgccaaaaga
     2101 tgatagtgag ctctatgcat cgatcgatat cgttcgcgat gctctcagtc cactgggaga
     2161 tataacagtg cagaccataa acccgccatc ttctcagaga ttccttatca gagttcgaga
     2221 taacggcgaa gacccgcagt ttatgacaaa tgttcctcag actatacgca atctcatcga
     2281 agataaggtc ggagccgata ggctagttgt ggtaaaagca gattatgtcg gacctcagta
     2341 ttccgaaacg cttagcagac aatcgatctg gctggtattg atgaccctcg tccttattct
     2401 tgtctattcg acaattcgat tcaaaataga atacgcaatc gctgctgtag cggcgatagc
     2461 tcacgatgcc cttgtgatga ttgcctttat agtatggaca aggatggaat tcaacaccac
     2521 aactatagca gcgattttga caatattggg ctattccata aacgacacca ttgtgcagtt
     2581 cgacagagtg cgagaggaac gcaagcttca tccttcggag ccatttgtcg atgtgctcga
     2641 cagggctttg acgctgactt taggacgctc cattataacg acgcttacca ccatgctggc
     2701 agtgctcgct ctctacttct ttacaagtgg aagtataaag gattttgcgc ttgcgcttct
     2761 tgttggcatg gttagcggtg tatactcgac aatttttata gccagcgcta ttgttctttt
     2821 cttggaaaac aaaaaaacaa agcgacgagc taaaaccatc aaagaaaaga ctgctccagc
     2881 aggcgcttta tctaaacaat aggtgtacac gatgggttta tgcaaagata gttgagctac
     2941 attactcaga attgttgaca aaagcgggga actcaagata ctatgtgccc cgcattcggc
     3001 gccgtaccca agtggtaagg gagaggtctg caaaaccttt atacatcggt tcgattccga
     3061 tcggcgccta attgagctgt gctgaaaatg ccacgagatt ttgtattgcc ctcgaataag
     3121 attcaagact tttgccgatt ttttgataaa agaatgcttc taaggcgcca gcagatgcga
     3181 aggtcacggt aacctatcag aaaggctcaa attttagcgc tcctttcccc aatggcattt
     3241 atgaagaaac attgaccatt tcggttgtcg ctgtctgaag attattcgat gctcctaatg
     3301 gaagggctgt gtacaaggtg acaggcagct attcaacgca gtattggctc tgtttagaat
     3361 gaatgttcgg taggtgaatg tggtgattgc gctttt
//
LOCUS       DAOMZL010000133         3364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_27321_length_3364_cov_17.846479, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000133 DAOMZL010000000
VERSION     DAOMZL010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27321_length_3364_cov_17.846479"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..360)
                     /gene="ssrA"
                     /locus_tag="PLQ30_07985"
     tmRNA           complement(4..360)
                     /gene="ssrA"
                     /locus_tag="PLQ30_07985"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(474..1595)
                     /gene="mnmA"
                     /locus_tag="PLQ30_07990"
     CDS             complement(474..1595)
                     /gene="mnmA"
                     /locus_tag="PLQ30_07990"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPW02426.1"
                     /translation="MNQDITIAVGLSGGVDSSLAACLLKEQGYKVIGLTMKIWKGAYK
                     IQENLKHSCFGPSEEEDIDACQRLCANLGIPYHVIDLADEYELHVLEYFRKEYLAGRT
                     PNPCIVCNRDLKFGFLIDKARQSGILFDMFATGHYARKSTINGLTYIRVARDVDKDQS
                     YFLYGLDSARLSYIIFPLGEMTKKETRQAAHFYGLEAAEKSESQDFISGGNYAPLFEN
                     EKPMPGDIVDLKGNILGRHRGLPFYTIGQRRGIGISLGAEPLYVLGLDVEQNRVIVGN
                     GKGLFSSGLIAKPFRLHNPQSFGQPIVCRVKIRQNHKPAEATAEVDKTGSARIEFMVP
                     QRAVAPGQSAVLYSEEGLVLGGGIIEKALPDIAIDTTEN"
     gene            1725..2852
                     /locus_tag="PLQ30_07995"
     CDS             1725..2852
                     /locus_tag="PLQ30_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPW02427.1"
                     /translation="MQDVFIGFTSITWKMLVMYGIGGILIWLAIKKQYEPMLLLPIGF
                     GSILVNLPLSIAWEHDGIPGFLRILYEAGIANELFPILIFIAVGAMIDFGPLFRNPLM
                     IFFGAAAQFGIFATMIVATLLGFDLKTAASIGIIGAADGPTAIYVANKFAKDYLAPIS
                     VAAYSYMSLVPIIQPPVIRLLTTKQERSIHMPLHEKQSPQWLKILFPIVVTMTSGIVA
                     PISVPLVGSLMFGNLIRESGVVERLSQVAQNELAYLVTLLLGICIGGSMSAEKFLNVD
                     TLLILAMGLVAFIFDTAGGVLFAKLVNLFLSKEKKINPMIGACGISAFPMSARVIQKM
                     ANDEERGNIILMQAIGANVSGQLGSIIAGGLVLALVPLIIG"
     gene            2863..3009
                     /locus_tag="PLQ30_08000"
     CDS             2863..3009
                     /locus_tag="PLQ30_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02428.1"
                     /translation="MEFIDNIRNAGTTIQAVAVSIGGLLGVFATLAFFFLMILLADRF
                     SKNR"
     gene            complement(3081..>3364)
                     /locus_tag="PLQ30_08005"
     CDS             complement(3081..>3364)
                     /locus_tag="PLQ30_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPW02429.1"
                     /translation="GILIAMPTYEYKMFPPMAYVLDIFERKHIYKRKSLRIGSYGWVG
                     GAKKEFEAKLESLKWSCIEAVEWPGAPDEATGELLKEKGRELARWVKAN"
BASE COUNT          812 a          743 c          777 g         1032 t
ORIGIN      
        1 aaaagctgga ggtgggggga attgaacccc cgtccccatg cgtgcgctgc acgcgtctac
       61 aggtttatct ggtcaatgta ttgtcggaag ccggggcgca gaccagcacg ctgtcggctt
      121 cttattccgg tttgatgtcc ctcataggca ccgaaccacc agtgaggtaa gctccctaat
      181 gtcacagaca tccatagcgt cggaagctgt ccgctatggc gtccgtcgct cactgcttat
      241 gcagcgagag cgagagcgcc gtttttgttg gcacttaaag gttttgccac gttaaggaga
      301 gtggctactc cacctgcagc gcacatcaca aaccgcatag gtcgaaacca gtgcaccccc
      361 aaaattatgc ttgttataag aataaaggaa ttctgttata gaatcaagcg ccttgccgta
      421 atgcttgatt ctattcgaga tttggtaaca gctccttgag agttttgagg tcttcagttt
      481 tcagttgtat cgatggcaat atcgggtaat gccttttcaa tgatgccgcc gccaagcacc
      541 aggccttcct cggaataaag caccgcagat tgaccaggcg caaccgctcg ttgaggaacc
      601 ataaattcta tgcgcgcact tcctgtctta tcgacctccg cggtagcttc tgcgggttta
      661 tgattttgcc tgatttttac tctgcagacg atgggctgtc cgaaagactg tggattgtgc
      721 agtctaaagg gtttcgcgat cagtccggat gaaaaaagcc cctttccatt tccgacaata
      781 acgcgatttt gttctacatc cagaccaagc acgtaaaggg gttctgcacc caaagaaatt
      841 ccaatgccac gtctctggcc aatggtgtag aatggtaatc ctcgatgtcg accaagtatg
      901 ttgcctttca aatccacaat gtcgccaggc attggttttt cgttttcaaa aagaggagca
      961 taattccctc cgctaataaa atcttggctt tcggattttt ctgctgcttc taatccatag
     1021 aaatgcgccg cttgacgtgt ttccttcttt gtcatctcgc cgagtgggaa tataatgtag
     1081 gataagcgcg cggaatccag accatatagg aaatagctct gatctttatc gacatctcga
     1141 gcaactctta tatatgtcag gccattaatt gtgctctttc ttgcgtagtg tcctgtggca
     1201 aacatatcga atagtatgcc agattgtctt gctttatcga tcaagaatcc aaactttaga
     1261 tcgcgattgc agactataca aggattgggc gttctgcctg caaggtattc tttacgaaag
     1321 tattctagca cgtgtagttc atactcgtcg gcgagatcta ttacatggta agggatgccc
     1381 aagtttgcgc acaagcgctg gcatgcatcg atatcctctt cttcgctggg gccaaaacac
     1441 gaatgcttga gattttcttg tattttatat gcacctttcc atattttcat ggtaaggcca
     1501 attaccttgt atccttgttc ttttaacagg catgcggcca gagaggagtc cactccaccc
     1561 gaaagaccga ctgcgatagt tatatcctga ttcatagcga gcgtacttta ctaggaatag
     1621 gttttttttt ccagcatcac catttgtagc taagattcaa atgtgtgcat ttttcttgga
     1681 gtttatactt gctttgttgt ccatatgcag ggtaaagtac ctgcatgcag gatgttttca
     1741 taggttttac ttccattaca tggaaaatgc tggtgatgta tggcataggt ggtattctca
     1801 tctggcttgc aattaagaag cagtatgagc caatgcttct gcttcctatt ggttttgggt
     1861 ctatacttgt aaatttgcca ctttcgattg catgggagca tgatggaata cctggatttc
     1921 ttcggattct ttacgaggca ggaattgcga atgaattatt tcctattctc atctttatcg
     1981 ctgttggagc gatgattgat tttggtcctc tttttagaaa tcctctcatg atattctttg
     2041 gagcggctgc gcagtttgga atatttgcga caatgatagt tgctacgctc ctgggtttcg
     2101 atctaaagac tgcagcctct attggtatta taggtgcggc cgatgggccg accgccatct
     2161 atgtggccaa taaattcgca aaggactatc tagcgcctat atcggtagct gcatattcat
     2221 atatgtctct ggtgcctatt atccagccgc cggtaataag gcttttgaca acaaagcagg
     2281 aacgatctat acatatgcca ctgcatgaaa agcaatcacc ccaatggctc aagattcttt
     2341 ttccaattgt agtgaccatg acctcaggta tcgttgctcc gatctccgta ccacttgtag
     2401 gttcgcttat gtttgggaat ttgatccgag agtcgggtgt ggtagaacga ctttctcagg
     2461 tcgcgcagaa tgagctagct tacctggtga cgctgctatt aggcatttgt atcggaggct
     2521 ccatgtcggc tgaaaaattc ctgaatgttg atactcttct catcttggcg atggggcttg
     2581 ttgcttttat attcgatact gcaggcggag tgctttttgc aaaactcgta aatctgttcc
     2641 tttcgaaaga gaagaaaatc aatccaatga taggggcttg tggcatttcg gcttttccaa
     2701 tgtcagcacg agtaattcaa aagatggcta atgatgaaga aagaggaaac ataatcctta
     2761 tgcaggctat aggcgcaaat gtatcgggtc aacttggttc gatcatagct ggtgggttgg
     2821 tacttgcgct tgtgcctcta atcatagggt aggagttata gaatggaatt tattgacaat
     2881 attcgaaatg caggtactac aatacaggca gttgccgttt ctatcggagg gcttcttggt
     2941 gtttttgcta cacttgcatt tttctttttg atgatcttgt tggcggatcg cttcagcaag
     3001 aatcgatgaa gacaaaagat agcagggcaa ttcttctaaa aagtcgattt cttagatgcc
     3061 tcttcggctt ggggtgacta tcagttggct tttacccatc gagcaagctc ccggcctttc
     3121 tcttttaaaa gctcacctgt ggcttcatct ggagctcctg gccattctac tgcttcgata
     3181 caactccact tgagggattc aagcttcgct tcgaattcct ttttggctcc tcctacccaa
     3241 ccatagcttc cgatgcgaag tgatttgcgc ttgtagatgt gtttgcgctc aaagatatcg
     3301 agtacataag ccattggtgg gaacatcttg tattcatagg taggcattgc aatcaatata
     3361 cctt
//
LOCUS       DAOMZL010000134         3296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_28118_length_3296_cov_13.828757, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000134 DAOMZL010000000
VERSION     DAOMZL010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28118_length_3296_cov_13.828757"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..965
                     /locus_tag="PLQ30_08010"
     CDS             63..965
                     /locus_tag="PLQ30_08010"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HPW02430.1"
                     /translation="MSKAFQKHSMRSLLYIPGNNPSMLQNCAIYGADAVIMDLEDSIS
                     ISEKDSARNLVKHALRDLDFGDMSIIVRVNARDTPYFEQDIEEIIPASPDAIRLPKVD
                     SASDVLAADELISRLEEINGLPKGKIGIQAMLETARAIINVNSIAASSPRLVGLTLGG
                     QDLAADLGIRATKEGYELIYAKSAVIIAAKAFGLQAFDTVFTDVNDPEGLRTQTELSF
                     ALGFSGKAAIHPSQVSIINSVYTPDPKELAKAIKIVTAAREAEQKGQGVIAVDGRMVD
                     APVVSQALRILELARLAGMEVSEL"
     gene            962..2524
                     /gene="citF"
                     /locus_tag="PLQ30_08015"
     CDS             962..2524
                     /gene="citF"
                     /locus_tag="PLQ30_08015"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HPW02431.1"
                     /translation="MSTNCVRNSLGRLVPREIRGLGAVEPFSGPYARLDGSFIWTASP
                     VSRRVPTISANKVVSSLRAAIERSGLQNGMTISFHHHLRNGDAIVSLVLSELEKMGYR
                     DLTLAPSSLGDSHDCVADAIKKGLVTRLNTSGVRGEVGKAISNGYLESPAIIRSHGGR
                     ARAIEEGSLKIDVAFLGAPACDRLGNMTGVIGNTACGSLGYAMVDARYASHVIAITDN
                     LVDYPLSPRISIPQYLVDQVVVVDSIGDPSKIATGATRITRDPLQLYIAELAFKVIKA
                     SGYLKNDCSFQTGGGGPSLAVARYVRDYMKSNSIKGSYCIGGITGYMADMLKEGLFRT
                     LFDVQSFDAAVIDSIAHNPNHVEADASWYANPFNKGCLVNDLDVVVLAALDVDIDFNV
                     DVLTGHDGILRGASGGHSDTAAGAKLALITIPSMRNGVPSIRERVQTIVTPGETIDVI
                     VTERGIAVNPRREDLIAKLKDSGLPIKNIHQLKKDIEAITGIPEEISFQNEIVGIVEY
                     RDGTIIDSIRKV"
     gene            2547..3035
                     /locus_tag="PLQ30_08020"
     CDS             2547..3035
                     /locus_tag="PLQ30_08020"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HPW02432.1"
                     /translation="MKEKIIIVAAILAGAWIGSKLKLPSGYLTGGLILGLIVKGIVGG
                     NVPSSGSVSIISQLLVAYVLVATSNVESLKQRPEVIPVALGYVVALLAFTTGLSFILN
                     RFFHIDLLTAMYSTAPGGISGMAITAVEAGADTAISSAFQVLRIVLVLVATPILAGIL
                     SK"
BASE COUNT          900 a          765 c          808 g          823 t
ORIGIN      
        1 attgtgttag ctggtgtatc acttgtatat gctttggtaa aaaggagaag acaagttgca
       61 aaatgagtaa ggcatttcag aaacactcaa tgcgttctct gctctacatc cctggcaata
      121 acccgagtat gctgcagaat tgtgcaatct atggcgcgga tgccgttata atggatctcg
      181 aagattcgat ttccatttct gaaaaggatt cagcgcgcaa ccttgtaaaa catgctcttc
      241 gagacctaga ttttggcgat atgagcataa tagttcgagt aaatgcaagg gacactccct
      301 attttgagca agatatcgaa gagattatcc cggctagtcc agatgcaata cgtttgccta
      361 aagtagattc ggcgtctgat gtgctcgccg ccgatgaatt gatatccaga ctggaagaga
      421 taaacggtct gccaaagggc aaaataggca ttcaagccat gctcgaaacc gcacgcgcaa
      481 ttatcaatgt aaattcgatt gctgcttcat caccacgtct cgtaggactg actctaggcg
      541 ggcaggattt ggcggccgat cttggcatac gtgccacaaa agagggttat gagctaattt
      601 acgcgaagag cgctgtcatc atcgctgcaa aagcatttgg gctccaagcc ttcgatactg
      661 tctttacaga tgtcaatgat cccgaaggtc tgcgaaccca gacagagctc agctttgctc
      721 ttggatttag tggaaaagcc gccatacatc catcgcaggt ctccattatt aactctgtct
      781 atactccaga tccaaaagag cttgcaaagg caatcaagat cgtcacagcc gctagagaag
      841 ccgaacaaaa agggcaaggg gttatagcgg tagacggacg catggtagat gctccggttg
      901 tgtcacaagc gctaaggatt ctcgaacttg cacgtttagc tgggatggag gtgtcagaac
      961 tatgagcacc aattgtgtac gcaactcgct tggcaggctc gttcctagag agattcgcgg
     1021 tctgggggct gtagaaccat tctcaggacc ttatgcaagg ctagatggtt catttatatg
     1081 gacggcctct ccggtctctc gaagagtgcc gactatcagt gccaataaag tagtctcaag
     1141 cctgagggca gcaatagagc gctccggttt gcagaatggt atgacaattt ctttccatca
     1201 ccatcttaga aatggcgatg caatagtaag ccttgtattg tccgaactgg agaaaatggg
     1261 ctatcgagac ctgacccttg cgccctcttc tctgggagac tcacatgact gtgtggcgga
     1321 tgcaataaag aagggactgg taacgagact gaatacctct ggagtccgag gcgaggttgg
     1381 caaggccatt tcgaatggct atctggagag ccctgcgata atacgaagcc acggcggacg
     1441 agcgcgagct atcgaagaag gcagccttaa aatcgatgtc gcattcctgg gtgcaccagc
     1501 ttgcgaccga cttggaaaca tgactggcgt aattggcaac acagcatgtg gctctctagg
     1561 ctatgccatg gtggacgctc gatacgctag ccatgtcatt gcaataaccg acaatcttgt
     1621 cgactatcct ctgtccccca gaatcagcat tccgcaatat ctcgtagatc aggtagtagt
     1681 tgtcgattcc ataggcgacc cttccaaaat agcaacaggt gccaccagaa taaccaggga
     1741 tccattacag ttgtatatag ctgagcttgc cttcaaggtc atcaaggctt caggctattt
     1801 gaagaatgac tgctcgttcc agacaggcgg aggcggacca agtctcgcag ttgcacgcta
     1861 tgtgcgggat tatatgaaat ctaactccat aaagggcagc tattgcatag gaggaataac
     1921 gggctatatg gcagatatgc tcaaagaggg cctctttagg acactctttg atgtacagag
     1981 ctttgacgct gccgtaatcg attccatcgc acacaatccg aaccatgtgg aagcagatgc
     2041 ctcatggtac gccaacccat ttaataaagg ctgtcttgtc aatgatctcg atgtagtcgt
     2101 actcgcagcc ctcgatgtcg atatcgattt caatgttgat gtcttaacag gccacgatgg
     2161 cattctgcgc ggagcatccg gaggtcattc ggataccgct gctggggcaa agctcgctct
     2221 gatcacaatt ccttctatgc gaaatggtgt cccttccata cgggaaagag tacagaccat
     2281 agtaactcct ggagaaacca tcgatgtcat cgttaccgag agaggtattg ccgtgaatcc
     2341 cagacgagaa gatctcattg ctaaactaaa ggattctggc cttcctataa agaacattca
     2401 tcagctgaag aaagatatcg aggctattac gggcattccc gaggaaattt ccttccaaaa
     2461 cgaaatcgtc ggaatagtgg aataccgcga tgggactatt atagactcaa tacgaaaagt
     2521 ttgataaaag cgccgccgga ggcattatga aagaaaagat aatcattgtt gccgcaattc
     2581 tggcaggagc ttggatcgga agcaaactca agctgccttc tggctacctt acaggagggt
     2641 taatccttgg ccttattgtc aagggaattg taggcggcaa tgttcccagc agtggatctg
     2701 tctcaataat ttcgcagctt cttgtggcat atgttctagt ggcaacatca aatgtggagt
     2761 cgctaaaaca aagacccgaa gtaattcctg tggctcttgg atatgttgtg gcgcttttgg
     2821 cattcaccac tggtttatct ttcattctca ataggttttt ccacatcgac ttgctgacag
     2881 ctatgtatag cacagcaccg ggcggcataa gcggaatggc tattaccgcc gtggaagccg
     2941 gagcagatac ggctatttcc tcggcatttc aggtgctgag aattgtgctc gttcttgtcg
     3001 ctacgcccat attagcaggg attctgtcaa aataattaaa gagagaaggc catcaagatt
     3061 cttatcaaga tccttatacg cacaagggaa aaatctatgg tctgtgacct ttggtcagtg
     3121 gtctttggac gctgcaaatt catgaaaatt tatgccctat tgttcgcagc gaattcatga
     3181 cagccaagag cgacacgccg acatccgcga tcaccgcctc ccacatattg gcaacgccaa
     3241 cgctcccaag tattatgaat gcccccttta ccgaaagagc aaaaacgata ttctga
//
LOCUS       DAOMZL010000135         3279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_28313_length_3279_cov_15.695099, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000135 DAOMZL010000000
VERSION     DAOMZL010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28313_length_3279_cov_15.695099"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="PLQ30_08025"
     CDS             <1..1286
                     /locus_tag="PLQ30_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02433.1"
                     /translation="VNSCAQAFDRVGLPEGQLHLVHATLYCALAPKSNSILCYFDALE
                     AVQSEQAEVPDHLKDANRDKVAFGHGEGYQYPHAYREHWVAQQYLPFSLMGRLFYIPG
                     ELGYEGTKRSDVLIRREAQLAIQPEDLNLIEDVNLIWSKKGKSRLSWRIRSESSTTDR
                     LLAIRTAFFNHLPLKPFDNIIVVDPRSGFYTMEALRRTREGKAFVLLGNKEAKMELEN
                     LSKTEEEIIQPEILTYGNFTTSGATSAALESFFSEAKPSFIILCEFCRVPIDVSEFLA
                     GLAGNVENGLLSFLAFDIDVETSSRLSSALLAMENLSNKEKEFCERLLAFENELGHGP
                     TYARLDSEKCSKQEWFEKKSSMGSSGFRKAILRASYSRPIDEKELERWLDSSTFYGSN
                     FQKAFSESETVMLKSLVSKFNRDPQWPLEILCFEF"
     gene            complement(1269..2291)
                     /locus_tag="PLQ30_08030"
     CDS             complement(1269..2291)
                     /locus_tag="PLQ30_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02434.1"
                     /translation="MRNIHAAEERQPAKSPIHRRGRGWGSSQKALSQSYESTKIDQPL
                     HLHDKQVVVIIESEGAVFNIHKYWHEMAYLPSFIGCFGGNVDPSIVGEVWRTVALETS
                     LRGENPIVILLAALRVLNALFPSMQRAVIIKTLEKYSLSEKTLEDLFNLNEKNPAEMM
                     IIDWFSMAEGLIQEIGRVPHFSTAEEFLHSVKYVAPRSEVLVYSRLAELSAMRMWQNA
                     GLGECFYRLAGRERGKPGDYLRAALSAGFERSSMVAIGASAAMFRAAQLAGIRFYPIT
                     PGLEEDAWKTLAEHWFPAYLRGEAWKVDMHAEPFYEQMCQEFNVMKTSRYIATRLHLT
                     SSKFEA"
     gene            2411..>3279
                     /locus_tag="PLQ30_08035"
     CDS             2411..>3279
                     /locus_tag="PLQ30_08035"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPW02435.1"
                     /translation="MSNRHFSSIDGVYDFVLGFVNVEKGQATEFKLDRMHWMAAQLGN
                     PHLGRLTVHVAGSKGKGSVATLIAKTLESSGLRTGLYTSPHILSWKERITESGIEMAD
                     ELLLKAAEEVYSLVEGRTQEDFPGNELPTFFELTTLIGFCAFRLAGFEAQVIEVGLGG
                     RLDSTNIVSPNVCVITPIELEHTQFLGSTIPLIAAEKAGIIKRSVPVCTIQPKTEAFR
                     VISKKAEDIGAPCLLVGRDISLSDIKINIHGTCCRLSATDTTPSNLLRLMQGASIEIS
                     TPLVGSIYAGNMAV"
BASE COUNT          922 a          765 c          715 g          877 t
ORIGIN      
        1 tggttaattc ctgcgcccaa gcctttgatc gtgtgggctt gcctgaaggt cagctccatc
       61 ttgtgcatgc aacactctac tgtgcacttg ctccaaaatc caactccatt ctttgctact
      121 tcgatgcctt agaagctgtg caatcagaac aggccgaggt tccagaccat ttaaaagatg
      181 caaatcgcga taaagttgca ttcggccacg gagaaggcta tcaatatcct catgcatatc
      241 gagaacactg ggtagcccaa caatatctgc ctttctcctt gatgggaaga cttttctata
      301 ttccaggcga gcttggatat gaaggtacaa agcgcagcga tgtcttaatc cgaagagaag
      361 cgcaactggc aatacagccc gaagatctaa acttgatcga agatgtgaac cttatatggt
      421 ctaaaaaagg aaaatctcga cttagctgga ggattcgcag tgaatcttcg acaaccgatc
      481 gcctccttgc tatacgcacg gcttttttca atcatcttcc gctcaagcca tttgataata
      541 ttatagttgt cgatccacga tccggattct ataccatgga agcgctgaga aggactcgcg
      601 aaggtaaggc ttttgttctg ctcggcaata aagaagccaa gatggagctt gaaaacttga
      661 gcaagacaga agaagaaata atccagcctg aaattcttac atatggaaat tttacaacat
      721 ctggtgctac aagtgctgca cttgaaagct tcttttcgga ggctaagcca tcttttatta
      781 tcttatgtga attctgccgt gtccccatcg atgtaagcga gtttttggct ggccttgcgg
      841 gaaatgttga aaatggatta ttaagctttt tagctttcga tatagatgtt gaaacatcgt
      901 cgcgactttc cagcgcacta ctggctatgg agaatcttag caacaaagaa aaggagtttt
      961 gcgaacgtct cttggctttt gaaaatgaac ttggacatgg tccaacctat gcaagacttg
     1021 actcggaaaa atgttctaaa caggaatggt tcgagaagaa gtcgagcatg ggctcttccg
     1081 gttttcgaaa ggccatactc agagcttcct attctcggcc tatagatgaa aaggaattgg
     1141 agagatggct ggattcctcg actttctatg gttccaattt ccaaaaagcc ttctccgaga
     1201 gcgaaacagt gatgctcaag agtctcgtaa gtaaattcaa cagagatcca caatggcctt
     1261 tagaaattct atgcttcgaa ttttgatgat gtcaaatgca aacgtgtagc tatgtacctt
     1321 gaagtcttca tgacattgaa ttcttgacac atctgctcgt agaatggctc agcatgcata
     1381 tcgactttcc atgcttctcc gcgaagatag gccggaaacc aatgttcagc caatgtcttc
     1441 caggcatctt cttccaggcc tggagtgatt ggataaaagc gaatgcctgc aagttgagct
     1501 gcgcgaaaca tagctgccga tgctccgata gctaccatgc tcgaacgctc gaaacctgca
     1561 gatagagcgg ctcggagata atctccaggc tttcctcttt ctctccctgc aaggcgatag
     1621 aagcattctc caagtccagc attttgccac attctcatcg ccgaaagctc ggcaagacgg
     1681 ctatatacaa gcacctctga acgaggagca acatacttta cgctatgtaa gaattcttcc
     1741 gctgtgctga aatgaggtac tcttccgatc tcttgaatca agccttcggc catagaaaac
     1801 caatcgatga tcatcatctc cgcaggattt ttttcgttca gattaaaaag atcttcaagt
     1861 gttttctcgg agagactata tttttccaga gtcttgatga tcacagctct ctgcatggaa
     1921 gggaatagcg catttagcac ccgcaaagcc gcgagaagaa taacaatcgg attttctccc
     1981 cgaagagatg tctcaagagc tacagtcctc cagacctcac ccacaatcga tggatcaaca
     2041 ttgccaccaa agcaaccaat aaaagacggc agataagcca tttcgtgcca atatttatga
     2101 atattaaaaa cggctccttc agattcgata ataaccacta cctgcttgtc atgcagatgc
     2161 agaggttggt ctatttttgt agattcatag gattgagaaa gtgctttctg cgaagatccc
     2221 caaccgcggc cccttcggtg tatcggggat tttgctggct gcctttcttc tgcagcgtga
     2281 atatttctca catcatcctc atctaagctt atcggcaagt tgaagctagc cttttatcaa
     2341 agcacaaatc tattttaaac aatagacaat ctcccttgac gaatttaagc gcattgcgca
     2401 tgctctatcc gtgagcaata gacatttttc atccatcgat ggggtctatg atttcgtcct
     2461 tggatttgtc aacgtcgaaa agggtcaggc aaccgaattc aagctggatc gcatgcactg
     2521 gatggccgca cagcttggca atccgcattt gggaagacta actgtacatg tagcgggttc
     2581 gaaaggcaag ggctcggtag caacattgat tgcaaaaaca ctcgaatcat ccggactgcg
     2641 aaccggactg tatacatcac cccatatcct ctcttggaaa gaacgaatca cagaatcagg
     2701 catagaaatg gctgatgagc tcttgcttaa ggctgcggaa gaagtctatt cccttgtcga
     2761 aggcaggact caagaagact ttcctggcaa tgaattgcca acattcttcg aattgaccac
     2821 cctgataggc ttttgcgcct ttcgtctagc tggtttcgaa gcacaggtta tcgaggtagg
     2881 attaggagga cggctggatt ctaccaacat cgtatcaccc aatgtatgcg taataacacc
     2941 tatagaactc gagcatactc aatttcttgg ttcgaccata cctctcattg ccgcagaaaa
     3001 agcgggaatc ataaagagaa gtgtacctgt ctgcactatt caaccaaaaa cagaggcgtt
     3061 cagagtgatc agcaagaaag ccgaagatat aggagctccc tgccttctgg tcgggagaga
     3121 tatctctttg tccgatataa agatcaatat ccatggcact tgttgtagat tatccgcaac
     3181 cgatactaca ccatccaacc ttcttcgact catgcaaggt gcttcgattg aaatcagcac
     3241 gcctttggtg ggctcgatct atgctggaaa tatggccgt
//
LOCUS       DAOMZL010000136         3277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_28344_length_3277_cov_16.517070, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000136 DAOMZL010000000
VERSION     DAOMZL010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28344_length_3277_cov_16.517070"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            470..1036
                     /locus_tag="PLQ30_08040"
     CDS             470..1036
                     /locus_tag="PLQ30_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPW02436.1"
                     /translation="MQYIAFDSHKRYTYAVVEDQEKGYRVMEQRITHRKGEIREFLSR
                     CERNSPVALETIGSWYWIVDEIEAAGMVPRLVNARKAKMMLASSKKTDRLDAKGLNTL
                     QRTGTLPTVWIPSGEVRDIRELFRTRMVLTREVTRLKNRILSCFSKYGISFDATSDAF
                     NVKGRASLEAARLLLPPHTSFAVQNLLG"
     gene            1046..1537
                     /locus_tag="PLQ30_08045"
     CDS             1046..1537
                     /locus_tag="PLQ30_08045"
                     /inference="COORDINATES: protein motif:HMM:NF014430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPW02437.1"
                     /translation="MKSAIKEFEARMHEVKNTAPEVALLQTIPGIGPILSHVIAYETG
                     DITRFPSAACYASYAGTTPSVHASGGKVRYGHTPADVNHYLHLAFVEAANILSMRHEA
                     LHFRHVAQLYERLKATKGHGIAIGAVARHLAEATYWVLSTKSAYREPHHTNCNTVVSS
                     KKG"
     gene            complement(2354..3124)
                     /locus_tag="PLQ30_08050"
     CDS             complement(2354..3124)
                     /locus_tag="PLQ30_08050"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02438.1"
                     /translation="MAEEVRADFRKVMEGLKEQLGASELLSRPVLKNKERVIEKMKED
                     GAKDASQIYDIDGHTLVFNDLESLSKAVDFFQKDPRVMRIKNYYANPTPLGYRGVNIN
                     VKLPNGMISEIQLNTKAMIEAKENYGHVFYEVAREAEGQLPPPPPPAPYHYAVLAQKK
                     LYAFAWEVSRGQQDSANLSASLFDISLPLWSMSAKILESHGSSWLSERTRKRFQDFGS
                     RAKGTSSLSKNLNDSKSEIEGMSKSSRIDKYTALALHA"
BASE COUNT          788 a          814 c          767 g          908 t
ORIGIN      
        1 gctttttagc aagaattaga ttttttggtt catattgtcg tggctcatct tatcctcccg
       61 gaagggtcat gcttattccc ccaagggtac ttgcataatt gcagcaccct cagaagcagc
      121 tgtcaagcgg aatatcgcga aggctctgtg ctatgcattg cctgctgtcg ctgataggag
      181 gccatagcgc gattttgcaa ggctaggccg tatattttca ccccttgcca aatagaagcc
      241 ccttgtaggc catttgtatt gttttgctaa tatttcggcg gttgaagaaa aagctgggtc
      301 attttgacct agcttttctg gcccccgttg accatttgcc ttcttcattc ggttatatct
      361 gcggtttttt gctagggctc attttgatga tatgagagcg ctgcaccagc gcgctatgtg
      421 aagtagaatt acttcattgg aagaatgagc gcaaaggagg tgcagcgaga tgcagtatat
      481 cgcatttgat tcgcacaagc gatacaccta tgcagttgtg gaagatcagg agaagggcta
      541 ccgcgtcatg gaacagcgca ttacacacag gaaaggcgag attcgtgaat ttctctcgcg
      601 ctgtgaacga aactctcctg tcgccttaga gacgataggc tcctggtact ggatcgtaga
      661 cgaaatcgag gcggcaggga tggtacctcg actggtgaat gcgaggaaag cgaaaatgat
      721 gcttgcctca tcaaagaaga ccgatcggct cgatgccaag ggactgaata ccctgcaacg
      781 aacgggaacc ttgcccacgg tgtggatacc ttccggtgag gtgcgtgaca tacgagagct
      841 ctttcgtacc agaatggtac tgaccagaga ggttacacgc cttaagaacc gcattctctc
      901 ctgcttcagc aagtatggca tttcctttga tgccacgagc gatgcgttca atgtgaaagg
      961 ccgcgcttct ttggaagctg cgcgtttact gcttcctccc cataccagtt ttgccgtaca
     1021 gaacctcctg ggatagctgg atgcggtgaa atctgcgata aaagagtttg aggcgcgcat
     1081 gcatgaagtc aagaacacag cgccagaagt agcacttttg cagacgattc caggcatagg
     1141 accgatcctc agccatgtta ttgcctatga aacaggcgat atcacacggt ttcccagtgc
     1201 tgcttgttat gcatcctatg caggcactac tccttctgtc catgcgtctg gaggcaaagt
     1261 tcggtatggg cacactcctg ccgatgtcaa tcattatctg catttggcat tcgtagaggc
     1321 agcgaatata ctctccatgc ggcatgaagc cttgcatttc cgtcatgttg ctcagctcta
     1381 tgaaaggcta aaggcaacaa aagggcatgg cattgcgatt ggtgccgtcg cacggcattt
     1441 ggctgaagcg acatattggg tactcagcac gaaaagcgcg tatagagaac cacaccatac
     1501 caactgcaat acggttgttt cgtccaagaa aggataagcg cgtgggggtc atggatcttc
     1561 aagatccgct tttttgattg cgaaatcctt tctggacatc atcatgtcgc agagacggcg
     1621 aagatatgaa tgcaacgaaa cagcgtaacg atgggtatgt gggtggcgat aattaataga
     1681 tgtcatcggt aaggagcata agagaagaaa aaattcatac ctttgttctt gacaggtgca
     1741 gcgctctcag atatgacccc caagctcagt gtaaaggttt tttgatttta ttgtaaagct
     1801 caatatcttc attaagcggc tgtgccgcag ccgcggccgc cgtaggcgtc ttaatggctg
     1861 gcaaagtttc acgttgcgaa acgccatccc catcagtatc caatgtccta tgtcctatgt
     1921 cctatgtcct atgtcctacg tcctaagtcc gctttccctc tcacttccca atcacttccc
     1981 tgtactgtcc caatcatttc cctctacata atcaaggaat tccgcacgct gttttcactc
     2041 ttggtccgct gtcgccgata ggctctgcca tagcaaggtt ttccgaaggc aagccatact
     2101 ttttcacccc ctgccaaaca gaagccccgc atcgcctccg aatttctctg cctttatagc
     2161 ttcctaagtg ctaaaaccgc gcgtcattca gtactcacta tgctgcggtt tctgcgatga
     2221 cccgatgtcg ttcttgcata ctgagcccca tatggtgcct ccaaaccatt gcatggagtg
     2281 tactttgggt tagattttat aatgacgcat cgatagcgtt tcgcttaaat tttcgatgag
     2341 gcaatgcgcc atgttacgcg tgtagcgcta atgctgtata cttatctata cgggaggatt
     2401 tagacatgcc ttcaatctca gactttgaat cattgagatt ttttgaaagg gacgacgtgc
     2461 cctttgcgcg cgatccaaaa tcctggaatc ttttccgggt gcgctctgaa agccaacttg
     2521 aaccatgcga ctcaagtatc tttgccgaca tactccaaag cgggagagaa atatcaaaaa
     2581 gcgaggcgct caaattggcg gaatcctgct ggcctcgcga cacttcccaa gcaaaagcat
     2641 agagcttttt ctgcgccaat actgcataat ggtatggcgc aggcggaggc ggagggggca
     2701 attgtccttc cgcttccctc gctacctcat aaaaaacatg cccataattt tctttcgctt
     2761 caatcatcgc ttttgtgttg agctgaattt ctgagatcat cccattcggt aattttacat
     2821 tgatattgac gccccggtat cccagcggtg tcggattggc ataatagttt tttatgcgca
     2881 tcacgcgagg gtctttctgg aagaagtcaa ctgctttcga cagactctca agatcattaa
     2941 acacaagcgt atgcccgtcg atatcgtaaa tctggcttgc gtccttcgca ccatcctctt
     3001 tcatcttctc aatcacgcgc tctttgtttt tcagtaccgg ccgtgaaaga agctcgcttg
     3061 cgccaagctg ttcctttaat ccttccataa cttttctaaa atcggcgcgt acttcttctg
     3121 ccattttgta gagagtatcg atatcgccgg aaggctgcaa aagttcatca cgcttacaag
     3181 gcttttgatt tgacggatca agaagccgct ttactgcttc cgactgttcg ccctttgttt
     3241 cgtctggata tccttcaact tcaactttca tattcgt
//
LOCUS       DAOMZL010000137         3220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_29120_length_3220_cov_17.418641, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000137 DAOMZL010000000
VERSION     DAOMZL010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29120_length_3220_cov_17.418641"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLQ30_08055"
     CDS             complement(<1..197)
                     /locus_tag="PLQ30_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02439.1"
                     /translation="MDRQIKSKQRVADHGEVLTGEREVNAMLDLVKPETERIDSRFLE
                     PACGTGNFLAEILRRKLRVVES"
     gene            complement(280..1239)
                     /locus_tag="PLQ30_08060"
     CDS             complement(280..1239)
                     /locus_tag="PLQ30_08060"
                     /inference="COORDINATES: protein motif:HMM:NF016924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HPW02440.1"
                     /translation="MNQFLEYARKVLESAEKPLLYQEIWEKGKGLGYDKDIATKGKTP
                     WQTLGAQLFVDVRDNPNSLFVKVAKRPARFFLKSKANTLTGEVLENLDSEVAIPKIKE
                     KEGFTEKDLHPLLAYFAYTNLQFSRGKAILTKTIYHEVSKKNGLSEWIHPDMVGVYIP
                     IDDWNSEIIELNNISNSNAITLYSFELKKSIDRSNYREYFFQAVSNSSWANEGYLVAA
                     NIKDDDELLNELERLSTSFGIGIIELDLEDIDSSRVIFQAHRKKELDWETMNKLSDIN
                     KDFRKFIQDVKIDFDSKRIHKSEYDEIISNPEEYIKKIMKKVS"
     gene            complement(1595..>3220)
                     /locus_tag="PLQ30_08065"
     CDS             complement(1595..>3220)
                     /locus_tag="PLQ30_08065"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HPW02441.1"
                     /translation="GYSCYTLCMETVLSELPDDVESLKKLVVEQARRAHELEATSKQL
                     EDEIAALKLKLQEVKDQYQLLQQKFFGSSSEKRKKKEEDNPKQPLLFNEAETYAEAPA
                     TPEKKTLVRSHERKVRGRKPLPENLERREFVHALSEAERTCPSCGAVRPEIGQEVREE
                     LEFIPARFVVNAHIIKKYGPCQCEVCANPIVQAEGPAKIIPGSSFSNNTIAFFLTSKF
                     VDAQPFYRMERILSRWGIETSRASLCKVAVSAGRAIGDLLDEMRKDLKASPVAQLDET
                     IVQVLHEKNRSAQAKSYMWVARGYADKKPIIFFHYHPSRAKEIAHRFLEGYQGFVQTD
                     GYSGYDEVGFFPGITHVGCLAHVRRKFFEAEKQGSKEASEFIELIAELYHAEKNLRQR
                     YEEGALTAEEFLSARTKEQGPRLARMKAWLISKQGQSPPSLSFGKAVNYALGQWDRIE
                     KYLGHELLTPDTNAVENAIRPFVIGRKNWLFSNTPLGAHASAGIYSLIETAKANGHEP
                     YRYLCYLFNELPKAKRLEEKRALLPYRLAPGSY"
BASE COUNT          745 a          824 c          673 g          978 t
ORIGIN      
        1 gattccacga cacgaagttt tcgtctgagg atttcagcaa gaaagttgcc cgtaccacag
       61 gcaggttcaa gaaagcgtga atcgatccgc tcggtctccg gctttaccag atcaagcatt
      121 gcgttgactt cacgctcgcc tgtcagcacc tcgccatgat cagcaactct ctgtttggac
      181 ttgatctgcc tatccatttg ttacaatcct gcttttcctt ataaatatta acgtatcctt
      241 aaactgaatg acagcaaccg agttatctca aagcaattgt tagctgactt tcttcattat
      301 ttttttaata tattcttctg gattactgat aatctcatca tattctgatt tgtgaatcct
      361 tttagaatca aaatcaattt tcacatcttg tataaacttc ctaaaatctt tgttgatatc
      421 gcttaatttg ttcattgttt cccagtctag ctcttttttt ctatgcgcct gaaatataac
      481 tcttgatgaa tcaatatctt cgagatctaa ttctattatg cctatgccaa aagaggttga
      541 taatctttcc agttcgttca acagctcatc atcatcctta atatttgcag ctactaagta
      601 tccttcattt gcccatgatg aattcgatac agcctggaaa aaatattctc gataattgct
      661 tctatcaata ctctttttta attcaaagga atataaggta atcgcatttg aattgctgat
      721 attattcaat tctattattt cactattcca gtcatcaata ggaatataca ctccaaccat
      781 gtctggatga atccattcgc ttagtccgtt cttttttgat acttcatggt atattgtttt
      841 tgttaatatt gctttgcctc tactgaactg taagtttgtg taggcaaaat aagcaagtaa
      901 tggatgcaaa tctttttcag taaatccttc tttttctttt atctttggga ttgctacctc
      961 agaatccaaa ttttctagta cttcaccagt taaagtatta gccttggatt ttaagaaaaa
     1021 tcttgcaggt ctttttgcga ctttgacaaa aagcgaattg gggttatccc ttacatcgac
     1081 aaagagctga gcacccaatg tctgccatgg tgtcttcccc tttgtagcaa tatccttatc
     1141 gtaaccaagt ccttttccct tttcccagat ttcctggtaa agtaatggtt tttctgcact
     1201 ttcaaggact tttcttgcat actctaaaaa ttggttcatt tttttcctct ttcgcgctga
     1261 gcgcccggct aatcattacg tttagctgtg aagcagaatt ggcgcggttt gtgcgagaag
     1321 gagctatacg acaacgcaca aaccatgaaa aaccgccgaa tctgcctgaa cgctttgtta
     1381 ggtccttttc atatccattc tagagacaga ttctattcgc ttttcctccc aagaatctct
     1441 tggacttcaa ctacattcac acctcttgta atagtgagca ttatctcatt ctctccatga
     1501 ttttggaaca gtatacaaac atcagacaac gagtatggat ctttgtactt tattccatat
     1561 gtgtaagcgc aaatttcacc ccgttgggca aaaatcagta tgatccaggc gcaagcctgt
     1621 acggcaaaag cgcacgcttc tcttccaggc gcttcgcttt gggaagttca ttgaacaggt
     1681 aacacagata cctgtatggc tcatggccgt tggccttggc tgtctcgatg agactgtaga
     1741 tccctgcaga cgcatgcgcg cccaagggtg tgttcgaaaa cagccaattc ttgcgtccta
     1801 tgacaaacgg ccggatcgcg ttctcgactg cgttggtgtc gggagtaagc agctcatgcc
     1861 caagatactt ctcgatcctg tcccattgcc cgagcgcgta gttcaccgct ttgccaaagg
     1921 agagactcgg aggactctgc ccctgcttcg atataagcca ggctttcatc ctcgcaagcc
     1981 ggggcccttg ttcctttgtg cgcgcagaga ggaattcctc cgcagtgagt gcaccttcct
     2041 cgtatctctg tcggagattc ttctccgcat ggtacagctc ggcgatgagt tcaataaatt
     2101 cactcgcttc tttgctcccc tgcttctccg cctcaaagaa cttcctgcgt acatgcgcaa
     2161 ggcagccgac atgggtaatg cctgggaaaa accctacttc atcgtagccg gagtatccgt
     2221 ccgtctggac aaagccctgg tagccttcaa gaaatctatg ggctatttcc tttgcccggc
     2281 tcggatgata gtggaagaat atgatcggct ttttgtccgc ataccctcgg gcgacccaca
     2341 tgtaactctt cgcctgcgca ctgcggtttt tctcatggag cacctggaca atcgtctcat
     2401 cgagttgtgc aaccggcgag gctttcaggt cctttcgcat ctcatcgagc aggtccccga
     2461 tcgccctgcc ggcggagacc gccaccttgc acaggctcgc gcgggaagtt tctatccccc
     2521 agcgggagag aatcctctcc atccggtaga agggctgggc atctacaaac tttgacgtaa
     2581 gaaaaaaggc aatcgtgtta ttcgagaagc tcgagccggg gataatcttc gcaggccctt
     2641 ctgcctgcac aatcgggttt gcgcacactt cacactggca ggggccgtat ttcttgatta
     2701 tgtgcgcgtt gaccacaaag cgtgccggga tgaattcaag ctcttcccgc acttcctgcc
     2761 cgatctcggg tcgtaccgcg ccgcaggaag gacaggtccg ctctgcttcg gacagcgcat
     2821 gcacaaactc gcgccgctcg agattctcag gcaaaggttt tcttccccga accttccgtt
     2881 catgcgatct gaccagtgtt ttcttttccg gcgttgcggg cgcttcggcg taggtctcgg
     2941 cttcgttgaa caggagtggc tgcttgggat tgtcttcttc tttcttcttt cgcttctcgc
     3001 tcgaagagcc aaaaaacttc tgctggagca gttggtattg gtccttgact tcctgcagct
     3061 tgagcttcag cgcggcaatt tcatcctcta gctgcttcga ggtcgcttca agctcatggg
     3121 cacggcgtgc ctgttctacc accagcttct tgaggctctc tacatcatcg ggaagctcag
     3181 acagcaccgt ctccatgcag agagtatagc atgaatagcc
//
LOCUS       DAOMZL010000138         3128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_30381_length_3128_cov_16.487797, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000138 DAOMZL010000000
VERSION     DAOMZL010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30381_length_3128_cov_16.487797"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..1603)
                     /locus_tag="PLQ30_08070"
     CDS             complement(248..1603)
                     /locus_tag="PLQ30_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW02442.1"
                     /translation="MNITINSENLAAIVRGCILSMCDYSCMVQSMGYAPESDRTDQSV
                     SRSSQLKEISFTINKLTAGAWMDDFEAARLIKEAIEPFASDIRESLFQSAPQGRLQES
                     PLGTARDAPQRTPEKAPEEVSKKASKGVVSLSIVTSKGRSIAHYTLIPLAPIDPHIRQ
                     EVNQPGDPSLLPSSARSEVVRGKISLVTGGAQGFGAEIARGLAFSGAAVWIADINLSG
                     ATCFAEKLRAETGTDAIFAVEIDVSKEESVERAFDTVARACGGLDLVVSNAGILRAGS
                     VLEQSAGEFRMVNDVNYIGFFNVAQHASRLLRRQWLGAPEWYTDIIQINSKSGLEGSN
                     RNAAYAGSKFGGIGLVQSFALELVDYNIKVNAICPGNFFGGPLWSDPLNGLFVQYLHS
                     GKVPGATTVEDVQGYYESKIPMRRGCYGPDVLKAIYYLVEQVYETGQALPVTGGQVML
                     N"
     gene            complement(1606..2973)
                     /locus_tag="PLQ30_08075"
     CDS             complement(1606..2973)
                     /locus_tag="PLQ30_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPW02443.1"
                     /translation="MNEPGELEKIINDAKNEPVLRLEVLAQLAGTMGRPAARSGESNN
                     HIHTCYSFSPYTPSGAALAARRAGLDVAGSVDHDSYAAASEMRAACALLGIGVVTGFE
                     LRVSLSEAAQSFPEKTSLMLTTHKLNNPDSIGIIYMTIQAIPAPAIKEVEAFLAPIRR
                     ARYQRSARMAELANKILFPLGLPEIDFQKDMVANSKYAEGGTITERHLLAAVSRSILS
                     QVEPGLELIKWLEQKLNIVLSNSQKRNLSESQNPYLVYDLLGALKSGFLDRIFIQPTE
                     ECVDVRDAISFAKKIGAIPAYAYLGDVDESPTGDKKAQKFEDEILDELIPVLKELGFP
                     AITYMPPRNTKEQLLRLQRLCRRYDLMEISGVDINQPRQSFRCPELGLPEFRHLDDAT
                     WAMVAHEALSGLDRRFGLFSAENSLAKMPLAERIRRYASIGRAIDIAEPGSLKKQAEL
                     SLLEK"
BASE COUNT          746 a          798 c          770 g          814 t
ORIGIN      
        1 caagaaacca ttcctccatg gccttgagat cgtgcgtttc aatgccatct cgcatgagtg
       61 gagggaagac ctttttcttt tctccgatca atgcaagttc gttggaatag agcacgactt
      121 ttttatttgt cattccgata tcgagcaccg caatcgcttc ggtcatagag cctcccttct
      181 cttaaaaacg aatggaccag gaaacattga aatatactag agagcgcgac catgcaacaa
      241 gaaaacgtca attgagcata acctgtccac ccgttacagg caaagcctgt cctgtctcgt
      301 atacctgctc aacaaggtag tagatcgctt tgagcacatc gggcccgtag cagcctcttc
      361 tcattggaat cttggattca taatatcctt gtacatcttc gactgtggta gcgccgggca
      421 ctttgccgga atggagatat tgaacaaaaa gcccattcag cgggtctgac cagagcggtc
      481 ctccgaaaaa gtttcctggg cagattgcat tcaccttgat attataatcg acaagctcaa
      541 gcgcaaagct ctgtacgagc ccaataccgc cgaatttcga tcctgcatag gcagcatttc
      601 tattcgatcc ttctaggcca gatttggaat tgatttgaat gatgtcggta taccattcgg
      661 gtgctccaag ccactgtcga cgtaaaaggc gcgatgcgtg ctgtgccaca ttgaaaaaac
      721 cgatatagtt cacatcgttg accatgcgaa actcgcctgc agactgctcg agcacagagc
      781 ctgctctcag gatgcctgca ttagacacaa caaggtcgag ccctccgcac gcgcgggcga
      841 ctgtatcgaa cgctcgttcg acagattctt ctttgctgac atcgatttca accgcgaaaa
      901 tcgcatctgt gccagtttct gcccgcagtt tttcagcaaa gcaggttgcg ccagatagat
      961 tgatatctgc aatccagacc gctgcgccag agaatgcaag tcctcgggct atctcagctc
     1021 caaagccctg cgctccgcca gttaccagcg aaattttgcc ccttacaact tctgatcgcg
     1081 cactgctggg gagaaggctt ggatctcctg gttgattgac ctcctggcga atgtgcgggt
     1141 cgatcggagc gagcggaata agggtatagt gagcaatgga tcggcccttt gaagtcacaa
     1201 ttgaaaggct caccacgcct ttggatgctt ttttggacac ttcctcgggc gctttttcgg
     1261 gcgttctttg gggcgcatct cgggctgtcc ccagtggcga ctcttgaagc cttccttggg
     1321 gcgcactttg gaataggctt tctcgaatat cgcttgcaaa tggctcgatt gcctctttta
     1381 tgagccttgc agcttcgaaa tcatccatcc atgcgccagc tgtgagctta tttatagtga
     1441 aagatatctc tttgagctgg ctactgcgag aaactgactg atcagtgcga tctgactcgg
     1501 gcgcgtagcc catgctttga accatgcagg aatagtcgca cattgaaaga atacaacctc
     1561 gcacaatagc tgcaaggttt tcagaattga ttgtgatatt catagctatt tctccaaaag
     1621 cgagagttct gcttgttttt taagcgaacc aggctctgca atgtcgatag cccggccaat
     1681 ggatgcataa cggcggattc gttcggcaag cggcatcttg gcaagggaat tctctgcaga
     1741 gaaaagacca aagcgccggt cgagcccact gagcgcttca tgcgctacca tcgcccaggt
     1801 agcatcatcc aaatgtcgaa attcaggcag tccgagctca ggacagcgaa atgattggcg
     1861 tggctgattg atgtcgaccc ccgatatctc catgagatca tatcttctac aaagcctctg
     1921 aagccgcaag agctgttcct tggtgttgcg aggtggcatg taagttatcg caggaaagcc
     1981 aagttctttt agcacaggaa ttagttcatc caatatttcg tcttcgaact tttgagcttt
     2041 tttgtcgcct gtcggagatt catcgacatc gccaagatat gcatacgcgg ggattgcgcc
     2101 aattttcttt gcaaagctga tggcatctct tacatcgaca cattcttcgg taggctgaat
     2161 aaagatccta tccaagaacc cggatttcaa ggctcccaag agatcgtaca cgagataggg
     2221 attctgagat tccgagagat ttcgcttctg agaattggaa aggacaatat tcagcttttg
     2281 ttccagccat ttaatgagct ccagaccagg ttccacctgt gacagaatcg atcgagatac
     2341 ggcagccagc agatggcgtt cggttattgt gccaccctct gcatatttcg agttagcaac
     2401 catatctttt tggaaatcta tctccggtag tcccaaggga aagaggattt tatttgccag
     2461 ctctgccatg cgcgcgctgc gctgatatct tgccctccta atcggagcaa gaaaggcttc
     2521 gacttctttg attgcggggg cgggtatggc ctggatggtc atatagataa tgcctataga
     2581 atcagggttg ttgagcttgt gggttgtgag catcagggat gtcttttcgg ggaagctctg
     2641 tgcagcttca gatagcgaga ccctaagctc aaaaccggtt acgaccccaa tgcctagcaa
     2701 ggcacaggcg gcgcgcattt cgctcgcggc cgcgtaggag tcgtggtcga cggaacccgc
     2761 gacatcgagg ccagccctgc gcgccgccaa ggccgcgccc gaaggtgtat aagggctgaa
     2821 gctgtagcag gtatgaatat gattgttcga ttcgccggat cgggcagccg gtctgcccat
     2881 tgttccggca agctgggcta aaacctcgag gcgcaataca ggctcatttt tggcatcatt
     2941 tatgatcttt tccagttctc ctggctcatt catcgcttat ttcctttttc ttgcatccag
     3001 cacgactcct caccatctca tactccaatt cgatatcggc gagcaagatc accagtggtc
     3061 tggatagtcg aagactcatg acccttgagt gggaggattc gttcgtttac cgcatctaca
     3121 atccattc
//
LOCUS       DAOMZL010000139         3086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_30970_length_3086_cov_9.566150, whole genome shotgun sequence.
ACCESSION   DAOMZL010000139 DAOMZL010000000
VERSION     DAOMZL010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30970_length_3086_cov_9.566150"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PLQ30_08080"
     CDS             complement(<1..737)
                     /locus_tag="PLQ30_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPW02444.1"
                     /translation="MKNKSRLTMAPISWRASAYIIMIFFTVITISPLIWLGYSSLKPN
                     AEIVQHPLALPQAPSLDNYIRAWNLGGLGTALKNSLIYTCVATISTVLLSLAAAFGLA
                     KFPFKSIKFFTWAFTVGLMLTVHAVIIPLFLAEVRIGLVNTRVGVILPYIAFNLPMSI
                     LIALGYVKGIPDALIESAEIDGAKYRQIFWSLIMPLSIPVVSTIAILSFLQYWNEFLF
                     VFIFTTKKALKSLPVAITQFAGRQNIE"
     gene            complement(746..1627)
                     /locus_tag="PLQ30_08085"
     CDS             complement(746..1627)
                     /locus_tag="PLQ30_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPW02445.1"
                     /translation="MVNARSKRRSYLSFVLPAFIIYATIMVFPLVFTLGLSFTKWKGY
                     GPIEFVGLRNYVKLLSDPVFHIGLRNNILIVLISVFGQIPLGFLLAYMLHRRMVKGAK
                     LFEIMIFLPITISSVIVAQLWNRVFAPVGILPTIVRQITGNQDYIMKIFESKKFAFVP
                     IMFVLLWQHTSLYMVIFLANLQRIPESIIEAAKIEGANEGTIFLKIIAPILAPVAFIN
                     SILAISGSFKSFDLIYSMTGGGPAHFTEVMAVYMYNTTFVFQNYGYGSAISIVIILFI
                     VLAIWLTKVVTNRLNMG"
     gene            complement(1705..3009)
                     /locus_tag="PLQ30_08090"
     CDS             complement(1705..3009)
                     /locus_tag="PLQ30_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPW02446.1"
                     /translation="MRSKMKVFIIALFALVTLVGLGAQNASSNAPVTLTVYMQNDLAN
                     PQSAYWPVTVEAFQKKYPNIKLEFEYVSGEEFHQKFQAMAASGDIPDLFTCYAGARSG
                     YILDRGMVKDLRPYLTDSFKSQYNSAIWQPQGPKGEIYIISPNMAVCTVVYVNTKLQK
                     QLGLSNPKTLDEMIAQVPKIRAAGLDPLAFGNKAQWQAQSLLLSMLVDRMGGTEWFDK
                     AMKGTAKFTDKPFVDAIAVIKKMVDAKLFPAGVNQLEGPQAWGEFVQGKAVYLLDAGW
                     RVSALKAAASPSDYAAYAAIAFPAVEGEVVHGSSAATLGEALAMNAKLSGAKADAAWK
                     FLSFIYGKEGSEILMQYGTVPTYKLDYSKFDIDSLNKEYINLINNQTMGYVIDAKMDS
                     EGVNNILNPGLQAVMMGTKTPAQLAAEYEAWVAANDSNRHKK"
BASE COUNT          817 a          663 c          799 g          807 t
ORIGIN      
        1 tattcgatat tctgtctgcc agcaaactgg gtaatggcta ctggcaagct cttcaacgct
       61 ttctttgtcg tgaagataaa cacaaaaagg aattcgttcc agtactgcaa gaaagagagt
      121 attgctattg tcgaaaccac aggaatggat aatggcatta ttagggacca aaatatctga
      181 cggtattttg ctccatcgat ttctgcagat tcaattagag catcgggaat gcccttaaca
      241 taaccaagcg ctataaggat cgacatagga aggttgaagg cgatatatgg caggatgact
      301 ccaactcgcg tgttaactag acctatacgg acttcggcaa gaaaaagagg gattataact
      361 gcgtgaaccg taagcatgag tccgacagta aaagcccagg tgaagaattt tatgcttttg
      421 aaagggaatt tcgcaaggcc aaaggccgct gccagcgaaa gcagaaccgt cgatatagtc
      481 gcaacacagg tgtagatgag gctgtttttg agggcagttc caagcccgcc caaattccaa
      541 gcgcgtatat aattgtcgag agatggtgct tgcggcagag cgagaggatg ttgcacgatt
      601 tctgcgttcg gtttgaggga ggagtaacca agccagatta gaggggaaat agttatgaca
      661 gtaaaaaaga tcatgattat gtaagctgaa gctctccagc tgatgggcgc cattgtcaag
      721 cgcgatttat ttttcatagc accccctaac ccatgttgag tctattggtc acgactttag
      781 taagccagat ggccaggaca ataaagagga ttatgacaat cgagatcgca ctcccgtatc
      841 catagttttg gaacacgaag gtggtgttgt acatatatac agccataact tcggtgaaat
      901 gggcagggcc acctcctgtc atcgagtaga tgaggtcgaa gctcttaaag ctgcccgaga
      961 ttgcgaggat cgaattgata aatgcaacgg gtgctaggat aggggcaatt atcttgagga
     1021 aaatggtgcc ttcgtttgct ccttcaatct ttgcagcctc gatgatcgat tcagggatgc
     1081 gctggaggtt ggctagaaag attaccatat ataggctggt gtgttgccat aggagcacga
     1141 acataatagg gacgaaagcg aattttttac tctcgaatat tttcatgatg tagtcttggt
     1201 tgccggttat ctggcgaaca atggtcggca ggattccgac gggcgcaaaa acgcgattcc
     1261 agagctgagc cactattacc gaggagatcg taattgggag aaaaatcata atctcgaaga
     1321 gttttgcgcc tttcaccatg cggcggtgga gcatataagc gagcaagaac cctaggggaa
     1381 tctgtccaaa tacagagatc aatacaataa ggatgttgtt cctgagtccg atgtggaaga
     1441 cagggtccga cagcagtttt acgtaattcc ggagacctac gaattcaatt gggccatatc
     1501 ctttccattt ggtaaaacta agacctagag tgaacaccag cggaaagacc attatggtcg
     1561 catagatgat gaaggctggc aacacgaagc ttaggtagct gcgtcgtttt gatcgtgcat
     1621 ttaccacatc gatgctccta ttcaattaca agttgtgggc tgggcgtttt tgcgcccagc
     1681 ccacaaatca gcaggaaata gacattactt cttatgtcga ttcgagtcgt tcgccgctac
     1741 ccaggcttcg tattcagctg cgagttgggc tggtgttttg gttcccatca taactgcttg
     1801 gagaccgggg ttaagaatat tattaacccc ttcgctatcc attttagcat cgatgacata
     1861 acccatcgtc tggttgttga tcaggttaat atattcctta ttgagcgagt cgatgtcgaa
     1921 tttggaataa tcgagtttat aggtcgggac agtaccgtac tgcatgagaa tctcgcttcc
     1981 ttccttgccg tatataaacg aaaggaattt ccatgctgca tcggctttgg cgccacttag
     2041 ctttgcattc atagcgagag cttcgccgag tgtggcagcg cttgaaccat gcactacttc
     2101 gccttcgaca gcggggaacg cgatagcagc atatgcagcg taatcggatg gagaagcagc
     2161 tgctttaagg gcagagacac gccatccagc gtctaggaga tagactgctt tgccctggac
     2221 aaactcgccc caggcttgcg gaccctcgag ctggtttacg ccagctggga agagtttagc
     2281 atcgaccatc ttctttatga cggctattgc atccacaaag ggcttgtctg tgaacttggc
     2341 agtgcctttc atcgccttgt cgaaccattc agttccgccc atacggtcta cgagcatgga
     2401 gaggaggaga gactgtgcct gccactgagc cttgtttccg aatgctaatg gatcgaggcc
     2461 agcagcgcgg atcttaggaa cttgagcgat catttcgtcg agagtcttgg ggttcgagag
     2521 accgagttgt ttctgaagtt ttgtattgac atagacgaca gtgcagacag ccatgttggg
     2581 ggatatgata taaatttcgc ctttcgggcc ttgtggctgc cagatggcag aattgtactg
     2641 gcttttgaag ctatcggtta ggtaaggcct gagatccttg accatgcctc tatcgagaat
     2701 gtaaccggat cgggcgcccg catagcaggt aaaaagatcg ggaatgtctc cacttgcagc
     2761 catggcttgg aatttctggt ggaactcttc gccggagaca tattcgaact cgagctttat
     2821 gttcggatac ttcttttgaa atgcttcgac agttacgggc cagtaggctg actgtgggtt
     2881 ggccaaatcg ttctgcatgt acaccgtgag tgttacgggg gcgttcgatg atgcgttctg
     2941 cgcacccaga cctactaagg tcaccagagc gaagagggca atgatgaaca ccttcatctt
     3001 ggatctcata aatacctcct gataatattt agatctctaa aattaaagat cgtagagccc
     3061 tatttcaatc gttttttacg actaat
//
LOCUS       DAOMZL010000140         2969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_32786_length_2969_cov_16.615305, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000140 DAOMZL010000000
VERSION     DAOMZL010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32786_length_2969_cov_16.615305"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PLQ30_08095"
     CDS             complement(<1..284)
                     /locus_tag="PLQ30_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HPW02447.1"
                     /translation="MGITIVFLGEIVGKTGVFTVKSTMPEIRQRFEPDFIFANANSAT
                     GCAGLGIQHAVYLRKLGIDCITMGEAAYYKPDMTEFYPKAGWVLRPANLP"
     gene            complement(376..861)
                     /locus_tag="PLQ30_08100"
     CDS             complement(376..861)
                     /locus_tag="PLQ30_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HPW02448.1"
                     /translation="MSEYRRPTWDEYFMEVANAIAKRATCDRGRSGCVIAKNNQILAT
                     GYVGAPSGLPHCDDVGHQMRKMLHEDGSITEHCVRTVHAEQNAICQAAKKGVAIEGAT
                     LYCRMTPCRTCAMLIINCGIVRVVAEYRYHDAEESEAMFKMAGIVLEYVHREVLQYSR
                     Q"
     gene            972..1991
                     /locus_tag="PLQ30_08105"
     CDS             972..1991
                     /locus_tag="PLQ30_08105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPW02449.1"
                     /translation="MKQRFMPLAFVLTLLAVPIQSCSAQTARPDGMYAIMKTNRGTIV
                     ITLEFEKAPMTVGNFVGLAEGSLDATKGKHFYDGLTFHRVVANFVVQGGDPVGDGTGG
                     PGYRFPDEIVPELKHDGPGVVSMANAGPNTNGSQFFITLEAAPWLDGKHSVFGKVIEG
                     MDVVKQIQQGDRIESITIQRVGTKAKSFDSSQQAWDQRLSAAYATLNALNKQKRDSDL
                     ELIAQKWPDLQVDENGIFQKIVRKGFGSMPAPGSKVSVIYKGMLLDGTVFDQSALSGS
                     PFAFTIGQGEVIDGWDLVISTMRKGEKRMIIIPPELAYGAQSIGDVIPPNSFLVFEIE
                     LVSIQ"
     gene            2236..2796
                     /locus_tag="PLQ30_08110"
     CDS             2236..2796
                     /locus_tag="PLQ30_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02450.1"
                     /translation="MRRGIKDISLGNVMLAIAAVILLAGIPSVNAQSTARKSISIQIV
                     AYVPAILNLTLDFSTSGATELVGYLGDTSDANGKGFELKSNSVVSLGAAHVISNLSSG
                     YSIVVQSMNGCALKNQDSQGTVKYDLLLGGIPALRQGDSFKLNCCGTTPMEGQMLPVS
                     IALGNVPSNATPGLYADNLLFNIMAN"
BASE COUNT          793 a          713 c          651 g          812 t
ORIGIN      
        1 tcgggaagat tagccggcct tagaacccat cctgctttag ggtagaattc agtcatgtct
       61 ggcttataat aagcagcctc acccatagtt atgcaatcga tccccaattt gcgaagatag
      121 accgcatgct ggatacccaa gcccgcacat cccgttgcag agtttgcatt tgcaaagata
      181 aagtcgggct cgaagcgctg cctgatctca ggcatagtgc tctttacggt gaatacaccg
      241 gtctttccga caatctcacc aagaaataca atcgttattc ccacaatgtg ctatgctagc
      301 gggaattcgc tatttctaca acgggcttct gcgaatattt caaaaaatag agccctttga
      361 ccggccttct ttcattcatt gtcttgagta ttgaagaacc tctctatgca catattcgag
      421 tacgatgcca gccattttga acattgcctc gctttcctct gcatcatgat agcgatattc
      481 agcgacaact ctgacaatgc cgcagtttat tatgagcatt gcacatgtcc ggcatggagt
      541 catgcggcaa tagagagttg ctccttcgat ggcaacacct tttttcgctg cctggcagat
      601 agcattctgc tctgcgtgca cggttcggac acagtgttcg gttatgctgc catcctcatg
      661 cagcatcttt ctcatctgat gacccacatc atcgcaatgc ggcaatccag aaggcgcacc
      721 cacatatcct gtagccagaa tctggttgtt cttcgctatg acacagcctg agcgaccgcg
      781 atcgcaagtg gctcgtttag caatagcatt ggcaacctcc atgaaatatt catcccatgt
      841 tgggcggcga tattccgaca tcagcttttc ctccttgccg acctcttcat ttataccgca
      901 taatatcata ggcaagatcg cagatcgatg cgaacatacg caaaatttat aacccattag
      961 gagtctttca gatgaagcag cgctttatgc cactcgcttt cgttctaaca cttctagcag
     1021 ttccgattca atcatgcagc gcacagacgg ctcgcccgga tggtatgtat gcaattatga
     1081 aaacaaatag aggaacgata gtcataaccc tcgaattcga gaaagcaccc atgacggtag
     1141 gcaattttgt cggacttgcg gaaggatctc tcgatgcaac caaaggcaag catttctatg
     1201 atgggctgac ttttcatagg gttgttgcca atttcgttgt gcagggggga gatcctgttg
     1261 gtgatggtac aggcgggccc gggtaccgat ttcccgacga aatcgtgcct gaactaaagc
     1321 atgatggacc tggcgtcgtt tctatggcca atgccggacc taacacaaat ggtagccaat
     1381 ttttcataac tctggaggcg gctccatggc tcgatggtaa gcactctgta tttggaaaag
     1441 tcatcgaagg catggatgtc gtgaaacaga ttcagcaagg ggatcgcatc gaatcaatca
     1501 caatccaaag agtaggcact aaggcaaaat ccttcgatag ttctcagcag gcttgggatc
     1561 aaagactcag cgctgcctat gccacactca atgccctcaa taagcaaaaa cgcgattccg
     1621 acctcgagct catcgcgcag aaatggcctg acctccaggt cgatgaaaat ggtatcttcc
     1681 aaaaaatcgt acgcaagggc ttcggatcta tgcctgcacc tggcagcaag gtttcagtga
     1741 tctacaaagg tatgctccta gacggcactg tcttcgacca atctgcgctt tctggtagcc
     1801 cttttgcatt taccattggg caaggagaag taattgatgg ttgggatctt gtaataagca
     1861 ctatgagaaa aggagaaaaa cgaatgataa tcatacctcc agagcttgcc tatggtgcac
     1921 agagcatcgg tgatgtaata ccgcccaact cctttttggt tttcgaaatc gagctcgttt
     1981 ccatacaata gcaaagcccc ggcctatatc aaaaatccat gagactaagg gatttgtcgc
     2041 ctcttatctc cttattagaa accgatttgg cttattggag ataatagaga ttagagccga
     2101 tatttctgtc cacttggaga taatggagtt tattcgataa aatatctcca ataactatta
     2161 tgatttattc ataattcaat aattatttca gtatttccgc aatactaatt ctgcttctct
     2221 ctctacaata atattatgag gagagggatt aaagacattt ctttaggaaa tgtaatgctg
     2281 gctattgctg cggtaattct gttggcgggc attccgtccg tcaatgcgca gtcaactgcc
     2341 aggaagagca tttctataca gattgtggcc tatgttcctg cgatcctcaa tctgactctg
     2401 gacttcagta caagcggagc cacagaactc gttggctatc taggtgatac ttcggatgca
     2461 aacggaaaag gttttgagct caaatctaat tccgtcgtct cccttggtgc cgctcacgtc
     2521 atttccaatc tatcctcagg ttactcaata gtcgttcagt ctatgaatgg ttgcgcattg
     2581 aagaatcagg attctcaggg cacggtcaag tacgatcttc tattgggcgg catcccagcg
     2641 ctgcgccaag gagacagctt taagctcaac tgctgtggaa ctacgccaat ggaaggccaa
     2701 atgttgccgg ttagtatagc attgggaaat gttccctcta acgccactcc tggtctctat
     2761 gccgacaatc ttcttttcaa cattatggcc aattgatgtc ggatctacta ccgtcttcct
     2821 ttcccagcca agcagagaac gagccaaaag aatttctcat ttcttcgatt ttttgataat
     2881 attcatcaat aaattactca cagaggaagg aatcatgacc aaagaaatcc agatgagttg
     2941 gctcgaagat atggcctttg aaagcttgt
//
LOCUS       DAOMZL010000141         2956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_32976_length_2956_cov_14.440882, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000141 DAOMZL010000000
VERSION     DAOMZL010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32976_length_2956_cov_14.440882"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PLQ30_08115"
     CDS             complement(<1..797)
                     /locus_tag="PLQ30_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02451.1"
                     /translation="MLTHRAMKIILSMLILLYIAQAPNAPSIYAQSTETQEKPKQTPY
                     IEGFEFHLKDVRETAVLRYLDMQIGQKFASQSDLDDFIAEKQRLLQENRVFNEEAAIW
                     YEQRNPSNPEAITIIVRGYATLTALAVPIPKYTEDDGFFLAFRYKDFNFLGMLEPLVF
                     SLDHYFLGGNTTIGAEFGYSPRFMNSQWEFSLTSSVDIGRNLDPAFSDTAISLSTEYP
                     ISEAPEYWAIVPSLSYTYTKSSKIHVGIASLSLIRPIFLLFPGNIEL"
     gene            complement(831..1931)
                     /locus_tag="PLQ30_08120"
     CDS             complement(831..1931)
                     /locus_tag="PLQ30_08120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW02452.1"
                     /translation="MRVLHIVTRSDSGGAQCMISTLAALQSQSGHIVAVASGLEGAGI
                     AWHSLPASIEKIPVRHLKRNIAPWDDLLALSEIRHICMGWKPDIVHIHTSKAAALTRI
                     AINPNRYPIIYTMHGYDQLKVANKKLLFIDKMLSPRTSAIVAVSHYDLRRMQTDGYHP
                     KLIANGVKAPSGQKSDASELTLALQLLEDLRKKESPTGLMIARPSSPKRPELVRESAR
                     QLKGLCSIVWIGGNARADDPPTFYALGPISDAGRLIPFCDFVLLPSDHEGLPMTLLEC
                     LAAGKPFIASNVGGIPELLKYADLPIAPGFAVENSAEDFASAIHSLADDIELRKAMGA
                     SGKKLWEKCYTAEIMAAEYESLYKHCKQIKKE"
     gene            complement(1931..>2956)
                     /locus_tag="PLQ30_08125"
     CDS             complement(1931..>2956)
                     /locus_tag="PLQ30_08125"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPW02453.1"
                     /translation="ILNWQIVIRDRWALCVIGFFLAIAAKDGIMLALGKGSFEAFAKS
                     GSRVVVLAGCSAMLYAHSREDIERALSVGFSVTFFYLFIIFIFMRFKILPMIYNANTF
                     GMVGIWAAILTGTNKWQNKTKNSKLQALAILISMFILVLQDAFFSGSFEGSRTAVFAM
                     IAALCFILLSETKYAIILARLELAVVLVFIFIGSMVFIPQFNDLLANRQYLWQAFLRK
                     GLEQPLLGWGFTTEAANIHLVSSYMIDTPFYDSFAKTGLGPHNSFLAMFFENGVIALA
                     GYITLLIARIRAIRVPLTMFDVSLLAYFIFMSLDAMAPGGLTVLGYFLGLCILAVPEK
                     AQKNKDT"
BASE COUNT          875 a          644 c          630 g          807 t
ORIGIN      
        1 tgcaattcta tgtttccagg aaagagaagg aaaatcggcc ttattaggct caagctagca
       61 atccctacat ggattttgct gctctttgta taggtatagg acagcgaagg aacaattgcc
      121 cagtattcag gtgcctcaga tataggatac tcagtcgaaa ggctgattgc agtatcgctg
      181 aaggctggat cgaggtttcg gccgatatca actgagctcg taagcgaaaa ctcccattga
      241 gaattcataa agcgaggcga gtatccaaac tcagcaccaa ttgtcgtatt tccacctaag
      301 aaataatggt ccaaggaaaa cactagtggc tccagcatac cgagaaagtt gaaatcctta
      361 tagcggaatg caaggaaaaa accatcgtct tcagtgtatt taggtattgg cacagcaagt
      421 gcggtcaagg tagcatagcc acgcactata atagttattg cttctggatt ggatggattt
      481 cgctgttcat accagattgc tgcctcctcg ttgaagactc tattctcctg aagaaggcgc
      541 tgcttttctg caataaaatc gtcgaggtcg gattggcttg caaatttctg gcctatttgc
      601 atatccaaat atcgaagaac tgctgtctct cggacatctt tcaaatgaaa ctcgaatccc
      661 tcaatataag gtgtttgctt aggtttttct tgtgtttcag tgctctgcgc ataaatgctt
      721 ggagcattag gagcttgcgc tatatatagc aaaatcagca tcgaaagtat tattttcata
      781 gcacgatgag tcagcattct aaacgctcct tatgcaattg cataattctt ttattccttt
      841 ttgatctgct tacagtgctt atagagcgac tcatattctg cagccatgat ttcagcagta
      901 tagcatttct cccagagttt tttgccactc gctcccattg ctttacgcag ttcgatgtca
      961 tccgcaaggc tatggatagc tgaagcaaaa tcctctgcgc tattctccac agcaaatcct
     1021 ggagctattg gtaaatccgc atacttaaga agctcgggga tgcctcctac attagaggcg
     1081 ataaaaggct tacctgcagc caaacattcg agaagggtca tcggcaaacc ttcgtggtca
     1141 gatggcaaga ggacaaaatc gcaaaaagga ataagtctgc cggcatctga aatcgggcca
     1201 agcgcataaa aggtgggtgg atcatcagct cgagcattgc cgcctatcca aacgatactg
     1261 caaaggcctt ttagctggcg agccgactct cgaactagtt cgggccgctt gggagaagag
     1321 ggtcgagcta tcattagccc tgtcggcgat tccttttttc gcaaatcttc tagaagctgt
     1381 agtgctaaag tcaattcaga agcatcggat ttctggccgc ttggagcctt tacgccatta
     1441 gctatcagct ttggatgata cccatcggtt tgcatgcgcc taagatcgta atgagagaca
     1501 gcaacaatcg ccgatgttct tggagaaagc attttatcta tgaaaagaag ctttttgtta
     1561 gcaaccttga gttggtcgta gccatgcatc gtatagatta ttggatatct attaggattt
     1621 atggctattc ttgtaagcgc cgcagctttg cttgtatgaa tatggacgat atcgggtttc
     1681 cagcccatac agatatgacg aatttcggag agtgctaata gatcgtccca aggtgctatg
     1741 ttgcgcttta ggtgccgaac tggtattttt tctatcgagg ccggaagaga atgccatgcg
     1801 attcctgctc cttccaagcc agaagcaact gcaacgatat ggccagattg actctgcaaa
     1861 gctgcaaggg tagagatcat gcattgcgcg ccgcccgagt cagatctggt aactatatgc
     1921 agaactctca tcaagtatcc ttgttctttt gagctttttc tggcactgca aggatacata
     1981 gtcctaggaa atatcctagt actgttagtc ctcctggagc catcgcatca agagacataa
     2041 aaataaaata ggctaaaaga gagacatcaa acattgtgag aggcactcgt attgcccgaa
     2101 tccgagcaat gagcaatgtt atgtatcctg caagagcgat aacaccattt tcgaaaaaca
     2161 tggccagaaa gctattgtga gggccaaggc ctgtcttggc aaagctatca tagaacggtg
     2221 tatctatcat ataggaactc acaagatgga tattagccgc ttctgtagta aatccccagc
     2281 caagcaaagg ttgctcgagg cctttcctta agaatgcttg ccaaagatat tgtcgatttg
     2341 ctaataaatc gttgaattga ggaatgaata ccatcgaacc gataaaaata aaaacaagaa
     2401 caactgcaag ttccaatcta gcaagtatga ttgcatattt tgtttcagat aaaagtatga
     2461 agcaaagtgc tgctatcata gcaaaaaccg cagtcctaga cccttcaaaa cttcctgaga
     2521 aaaacgcatc ttgaaggacc agtatgaaca tcgagataag aattgctaga gcttgcagct
     2581 tactattttt tgttttattt tgccatttat tcgttcccgt aagaattgcc gcccatatcc
     2641 ctaccattcc aaatgtattt gcattgtaaa tcataggtaa tattttgaat cgcataaaaa
     2701 taaaaatgat aaacaaataa aaaaaagtaa ccgaaaatcc aaccgagaga gctctttcga
     2761 tgtcctctct tgaatgagca tatagcattg cactacagcc tgccagtaca acaactcgag
     2821 atcctgactt agcaaatgct tcgaagctac ctttccccaa agcaagcata ataccatctt
     2881 tggctgcaat cgccaggaaa aaacctatca cacataaggc ccatcgatct cggataacaa
     2941 tttgccaatt caagat
//
LOCUS       DAOMZL010000142         2943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_33184_length_2943_cov_13.742729, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000142 DAOMZL010000000
VERSION     DAOMZL010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33184_length_2943_cov_13.742729"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="PLQ30_08130"
     CDS             <1..1070
                     /locus_tag="PLQ30_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPW02454.1"
                     /translation="IVLDMQVIPFNDYENKLRMMIAGGNPPDVARLASAHVPLFATSL
                     EPLSGKSEFDELAKSYFDSSLALGMDENGRIMAMPTEATANGMIVNKTYFKNAGIDID
                     KLSLTWTWDQWVDAMKKVIKANPKAKYGITVDFSTHRFATFLYEAGGRFLSSDGKSMN
                     FNTPESLDALKFFKMLHDEGLAPKSVWMGSENPQELFQSGLVACHVGGSWLINAYNKN
                     IKDFEWAAVRMPKRKINSSVPGGKFIGTFKSSAHKKEALDLIAVFSDKAHNEQYCKDT
                     FNLSARKDANIKYTARSNDFAVFTAELNVTPPYTADDWKSDAVAKVNSYAREQIVEGL
                     LGKQTMEQTAANIQAKGNSYF"
     gene            1211..2104
                     /locus_tag="PLQ30_08135"
     CDS             1211..2104
                     /locus_tag="PLQ30_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPW02455.1"
                     /translation="MKSKQRSHGTFEQTLAPLLFLTPNLLIFSIFIIVPAIMGLRMSF
                     FDWSILEGSRFIGFQNFKSISHDSMFWLTLKNTLVYVGAVVPLLTVFALALALPLSKE
                     DRGMGVFRVIYYIPTMLSMIVVGISWRWLLGYDLGIINYVISLAGGKPVPWLTNSTMA
                     NICLIFVTLWTRVGYFMMMFVGGLQAIPVTYYEAAKMDGAGRSTIFRRITLPLLSPTI
                     LVVVVLATIEAFKAYELIFVMTQGGPGTSTKFIVQYIYQVAFQEDKMGYGAAMSVILL
                     IIIGIFTALQFSIQKEEYTNE"
     gene            2097..2906
                     /locus_tag="PLQ30_08140"
     CDS             2097..2906
                     /locus_tag="PLQ30_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPW02456.1"
                     /translation="MNRALKYILLIIVSGIFLFPILWVIISSFKPQSELFTYPLTILP
                     HKATIENYVNAFASGDFITYYANSIFVSVAATLLTITINVMAGYALSKFYFKGRDLIF
                     YIMISTLMIPLQAIMIPIFLLLKELGLLNSLWGIIIPPAATPTGVFLARQYIQTIPNS
                     YIEAARMDGASELRIFLRIIVPLAQPIIATIAIFSFMWRWNDFLWPFIVISSKRKMTL
                     QLALANFVGQFQINWGNLLAMTVVTMIPMIVVFLVLQRYFVQGLSAGGVKA"
BASE COUNT          808 a          609 c          630 g          896 t
ORIGIN      
        1 acatcgtcct cgatatgcag gttataccct tcaatgatta tgagaacaag cttcgaatga
       61 tgattgcggg tgggaatcct cctgatgttg caagattggc aagcgcacat gttccgcttt
      121 ttgcaacaag tcttgaacct ttgtctggca agtccgaatt cgatgaacta gcaaaatcct
      181 attttgattc ttctctggcg ctgggcatgg atgaaaatgg tcgaataatg gctatgccaa
      241 ccgaagcaac agcaaatggg atgattgtaa acaaaaccta tttcaaaaat gcgggtatag
      301 atatcgataa attgtcactt acatggacat gggatcaatg ggtagatgcc atgaaaaaag
      361 ttatcaaagc aaatccaaag gcaaaatatg gcataacagt ggatttttca acgcatcgat
      421 ttgctacctt cctatatgaa gctggcggac gatttttgtc gagcgacggg aagtcgatga
      481 atttcaatac gccggagtct ctcgatgcgc taaaattctt caaaatgttg catgatgaag
      541 gtcttgcccc caaatcggtg tggatgggct cggagaatcc ccaagagctt ttccaatctg
      601 gcttagtagc ctgtcatgtg ggcggttcat ggctcataaa tgcctataac aagaatatca
      661 aagattttga gtgggcagcg gtaagaatgc caaaacggaa gatcaattcc tccgtgcctg
      721 gtggcaaatt cattggtacc ttcaagagct cagctcataa gaaagaagca ttggatctca
      781 tagcggtttt ttctgacaaa gcgcacaacg aacaatactg caaagatact ttcaacctgt
      841 ccgcacgaaa agatgctaat atcaagtata ccgcaaggtc caatgacttt gccgtcttta
      901 ctgcggaact caacgtaacc cctccctata ctgcggacga ttggaaaagc gacgctgtgg
      961 cgaaggtcaa ctcctatgcg cgagagcaga tcgtagaagg gttgttagga aagcagacaa
     1021 tggagcagac tgctgccaat atacaggcca aaggcaactc ctacttctga taaaatgttc
     1081 gttatttcta aattgacgta agaatttatc ttacgataga tttttaattg tttctgctgc
     1141 atgcgttgaa caagaatgca tgcagcagcc tgttttttgt agaggatcaa ttattacaga
     1201 gaaataaagg atgaaatcta agcaacgatc tcatggcaca ttcgagcaaa cgctagcacc
     1261 tctcttattt cttacaccga atttgctgat tttctcaata ttcatcattg tcccggctat
     1321 catgggactt cggatgtcgt tttttgattg gagcatctta gagggttcca gatttattgg
     1381 tttccaaaac ttcaagagta tatcacatga ttcgatgttc tggcttactc taaagaatac
     1441 gcttgtctat gttggtgctg tcgttcctct tctgacggtt ttcgctctag ctctcgcgtt
     1501 gcctctgtcc aaagaggata gaggcatggg tgtttttcgt gtcatctatt atataccgac
     1561 catgctaagc atgattgtag tgggcatttc ctggcgatgg ctcctgggct atgatcttgg
     1621 cattatcaat tatgtaatta gcctggcggg aggaaagccc gtaccatggc ttactaatag
     1681 cacaatggcc aatatttgcc tcatttttgt aacgctttgg acaagggtag gatattttat
     1741 gatgatgttc gtgggtggac ttcaagcgat tcccgtaacc tattatgaag ctgctaaaat
     1801 ggatggggca gggagatcga ctattttccg acggatcacg cttccacttt taagtcctac
     1861 cattcttgtc gtcgtagtgc ttgccaccat cgaagccttc aaggcatatg agctcatttt
     1921 tgtgatgact cagggcggac ccggaacatc caccaaattc attgttcagt atatctatca
     1981 ggttgccttt caagaggaca aaatgggata tggcgctgcc atgtcggtaa ttcttcttat
     2041 tataattgga atattcacgg ctcttcagtt cagcatccag aaggaggaat ataccaatga
     2101 atagagcact caaatatata ctgttgataa tagtctcagg tatatttcta ttccctattc
     2161 tatgggtcat tatctcttct ttcaaacctc aatcagagct ttttacttat ccactgacca
     2221 ttctgcctca caaggcaacg attgagaatt atgtcaatgc atttgcaagt ggtgatttta
     2281 tcacctatta tgcaaattcc atttttgtct ctgttgctgc aacattgctt accataacaa
     2341 taaacgttat ggcaggttat gctctttcca agttctattt caaaggtcga gatctgattt
     2401 tctatatcat gattagcacg ctgatgattc ctttacaggc gattatgata ccaattttct
     2461 tgcttctgaa agaattaggt ttgctaaatt ctctgtgggg aatcatcatt cctcctgccg
     2521 caactccgac cggtgtattt cttgcccgtc aatacattca gacaattcca aattcgtata
     2581 tcgaggcggc acggatggat ggcgcaagcg aattgcgcat ttttttaagg atcattgtac
     2641 cattagctca gccgattatc gcgacaattg caatcttttc tttcatgtgg cgatggaatg
     2701 actttctttg gccattcata gttatttcat ctaagagaaa aatgactttg cagcttgcac
     2761 tagccaattt tgtggggcaa ttccagataa attggggcaa tctacttgca atgaccgtag
     2821 tgacaatgat acctatgatt gttgtttttc ttgtcttaca gcgatacttt gtccaagggc
     2881 tcagcgccgg cggagtaaag gcataaatat gctatatgtc ataaaaaatc tcagttcata
     2941 tca
//
LOCUS       DAOMZL010000143         2917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_33599_length_2917_cov_17.854298, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000143 DAOMZL010000000
VERSION     DAOMZL010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33599_length_2917_cov_17.854298"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /locus_tag="PLQ30_08145"
     CDS             <1..122
                     /locus_tag="PLQ30_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPW02457.1"
                     /translation="HRIDLTLYKLDLIMEGDLDELLDALIAWDREQALMAEER"
     gene            128..997
                     /gene="prmC"
                     /locus_tag="PLQ30_08150"
     CDS             128..997
                     /gene="prmC"
                     /locus_tag="PLQ30_08150"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPW02458.1"
                     /translation="MNYRELILNESSSFSDSDSPFLDAVLLMAHSLGITKEKLFSMLS
                     ERVDSVPQSFFDMTERRRNGESIAHIRGFREFYGREFLVNSDVLSPRQDTEVLVEAVL
                     ESSKLFEPSAGINGSLDVVREISILDLCTGCGAVAISLAAEMPNHKVTASDISSAALD
                     LAKKNAQRLLPAQSLIFIESDLFSEIDGKFEIIAANPPYVPHYQAEQLIAKGWKDPLI
                     SLDGGIDGMEYIRKIIMEGYEYLSHNGVLLLEMDPHQASESLALFGEMGYIDLKTWKD
                     LTGSIRVVGGRHG"
     gene            990..>2917
                     /locus_tag="PLQ30_08155"
     CDS             990..>2917
                     /locus_tag="PLQ30_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HPW02459.1"
                     /translation="MDEFVNEFSSKIEHRFNDEEKNLLINALDHAHSVHGERKRASGE
                     PAYYHDIRVAEILLDLGMDLDSILAGLLHDTIEERISGFVQDANLDRNIEGKPANREK
                     SALEESRISPRGIKQTTKGEHALKKKSSNALSSLLAAPDPFIEERFGPQTAVIVAGVN
                     RLSSVKAKNKTVHAAETMRKMLFALTSDIRVILVKLADKLDSMRTLKYLPEDRQKEIA
                     TECIDIYAPLADRLGISWLKDELEDLSLKALNREVFDQIKEIVNSRKHERQDFLKKVT
                     DKILSTAAKEGIDVEVSARAKHFYSIYQKMRRRGKGAEELYDLLGIRIICNGINDCYA
                     LLGLVHRLWKPIDGRFKDYIAMPKANGYQSLHTTVIAYGGQLLEIQIRTREMHLVAEY
                     GVASHWLYKKGTGAEIPKLEDLPIMNKLRQWNQFIAEGDSYLEEIKQEFLGDSIFVFT
                     PKGDIIELPSGSTPIDFAFAIHSDVGAHCLGAKINGAIAPLDSELRNTQIVEIITSPN
                     ARPNLNWLKIARTSKARSKIRQLLVQTGQAVAIDKHIVAARKSEKAAQETVQARVHGA
                     DRSQIEARRGEFPSVVEFHSLKPGLVLRSEKVGVSVGGMRNLMIRIAGCCKPVTGDRI
                     VGYVSRGRGIIVHREDCRS"
BASE COUNT          824 a          629 c          720 g          744 t
ORIGIN      
        1 accataggat agatttgact ctatataagc tcgacctcat catggaaggt gatcttgatg
       61 aattgctcga tgcgctgata gcatgggata gagaacaggc actgatggcc gaagaacgct
      121 aattgtcatg aattaccgag aacttatctt gaatgaatct agcagctttt ccgatagcga
      181 tagccctttt ctggatgcag ttttgctcat ggctcatagc ctcggtataa ctaaggaaaa
      241 gcttttctca atgctctctg agcgtgtaga ttctgtccca caatccttct tcgatatgac
      301 agaacgcaga agaaatggcg agagcatagc ccatattcgc ggtttccgtg agttctatgg
      361 tagggaattt ctagtcaaca gtgatgtatt atcgccccgg caagacacag aggtcttggt
      421 agaggctgtg cttgaatctt caaagctatt cgagccctca gcaggcataa atgggagtct
      481 tgatgtagtc cgagaaattt ccattctcga tctttgtacc ggttgcggcg cagtggcaat
      541 cagtctcgca gccgaaatgc caaatcataa ggtaacagcc tcagatatat cttctgcagc
      601 tctcgacctt gcaaagaaga acgcgcaacg gcttttgccc gctcagtcct tgattttcat
      661 agaatccgat cttttttccg aaatagatgg aaagttcgaa attattgccg caaatcctcc
      721 ttatgttccg cattatcaag cggagcaatt gattgccaaa ggatggaagg atccccttat
      781 ctctctggat ggaggtatag acggcatgga gtatatccgg aaaataatca tggagggata
      841 tgaatattta tcgcataatg gcgtattatt attggagatg gatccacacc aggcatcgga
      901 gtctctggct ctctttggag agatgggcta tatagatctt aaaacatgga aggatctcac
      961 aggaagtata agggtagtag gcggacgcca tggatgaatt cgttaatgaa ttttcttcta
     1021 agatcgagca tcgtttcaat gatgaagaga agaatcttct tataaatgct ctggatcacg
     1081 ctcatagtgt tcatggagaa agaaaacgag catctggtga gcctgcttat tatcatgata
     1141 tcagggttgc tgaaattctc ctcgaccttg gtatggatct tgactcaatc cttgctggtc
     1201 ttctccacga tacaatagag gagcgaatat cgggttttgt ccaggatgcg aatttagata
     1261 gaaatatcga aggaaaacct gcaaacagag aaaaatcggc attggaggag tcccgaattt
     1321 cgccaagagg gataaaacag actaccaaag gcgaacacgc attgaagaaa aaaagctcaa
     1381 acgcattgag ttcccttctt gcggcgccag atccttttat tgaggaacga tttggtcccc
     1441 aaaccgcggt gatcgttgcc ggagtcaaca ggctgtcatc ggtcaaggca aagaacaaaa
     1501 ccgtgcatgc tgctgaaaca atgcgaaaaa tgcttttcgc tttgactagc gatattcgtg
     1561 ttatcctcgt aaaactcgca gataagctag atagcatgcg cactctgaaa tatctaccgg
     1621 aagatcgcca gaaagagata gctaccgagt gcatagatat ctacgctcct cttgccgata
     1681 gactgggtat ttcctggcta aaggatgaat tagaggattt gtccctgaaa gctctaaacc
     1741 gtgaggtgtt cgaccagata aaggaaattg taaattcgcg caagcatgag cgtcaggact
     1801 ttctaaagaa agtgacggat aagattctct caacagctgc aaaagagggg atagatgtcg
     1861 aggtcagcgc tcgtgctaag catttttact ccatatatca gaagatgcgc aggcgcggga
     1921 aaggtgccga agagctatat gaccttcttg gcatacggat tatctgcaat ggcataaatg
     1981 attgttatgc tctgcttgga ctcgtgcacc ggctctggaa gcccattgat ggtcgattca
     2041 aagactatat tgccatgcca aaagcaaatg gataccaaag cctccataca acagtgatcg
     2101 catatggggg gcaactgcta gagatacaga ttcgaactag agaaatgcac cttgtggcgg
     2161 aatatggagt agcatcgcat tggctgtaca agaaaggtac cggggcagag attccaaagc
     2221 ttgaagattt gccgattatg aacaagctaa ggcagtggaa ccagtttatt gccgaaggcg
     2281 acagctatct agaagaaata aagcaagagt tcctgggtga ttctatattt gtctttaccc
     2341 caaagggaga tataattgag ttgccttctg gttcgacacc tatagacttt gcattcgcta
     2401 ttcactccga cgttggggca cactgccttg gcgcaaagat caacggtgcc atagcacctc
     2461 tcgattccga gcttcgcaat actcagattg tcgagatcat tacttcaccc aatgcaagac
     2521 ctaatctcaa ttggctcaag atagcccgca ctagtaaagc acgctcaaag atacgccagc
     2581 ttcttgtaca gactggccag gccgtggcga tagacaagca catagttgcg gctcgcaaga
     2641 gcgaaaaagc tgctcaggaa acggttcagg ctcgcgttca cggagccgat cgatctcaaa
     2701 tcgaagctcg ccgcggcgaa tttccttccg ttgtggagtt ccatagccta aagcctggcc
     2761 ttgtgcttcg cagtgagaaa gtgggcgttt cggtgggagg catgcgcaac ttgatgattc
     2821 gcatcgcagg ctgctgtaaa ccggttactg gcgatcgtat cgtaggctat gtttcgcgtg
     2881 gtcgtggaat tatcgtacat cgcgaagact gcagatc
//
LOCUS       DAOMZL010000144         2915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_33641_length_2915_cov_12.831818, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000144 DAOMZL010000000
VERSION     DAOMZL010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33641_length_2915_cov_12.831818"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLQ30_08160"
     CDS             <1..368
                     /locus_tag="PLQ30_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPW02460.1"
                     /translation="IVRLIGPDALPDEQRLILLTADMIRNGFLQQSSFDRVDMYCAPQ
                     KQTLLLQCILTFHELAETAIKNGAPLPKVSALPIREKIVRLKSSLENDKVEEGRMVIQ
                     EIQVAFEQLGVTAQGAVLA"
     gene            365..1819
                     /locus_tag="PLQ30_08165"
     CDS             365..1819
                     /locus_tag="PLQ30_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPW02461.1"
                     /translation="MRGLEYHGIERADGPIIIAERREDIGFSELVRVKDRQGEYRLGR
                     VVELSDKAVAIQLFAENTGISIDDAWVEYLEKPLTFRVGEGIVGRIFNGLGEPIDGYP
                     PILSSDLRDINGLPINPSARVYPRDFIQTGISAIDGMNTLIRGQKLPIFSGNGLPHNR
                     LAAQIVRQAKVLSGESQFAIVFAGMGIKYDVARFFIDSFESSGVLGRVVMFLSLADSP
                     SIERLVTPRTALSAAEYLAYEKNMHVLVVMTDMTNYCEALREVSAARNEVPSRKGYPG
                     YLYSDLASIYERAGRIEGSEGSITQLPILSMPNDDISHPIPDLTGYITEGQIVLEREL
                     SQRGVYPPIAGLPSLSRLMKDGIGEGMTREDHRELAAQLFSAYAYVHTVRDLAAIIGE
                     EELSDRDKIFLNFGDHFESQFLAQGEFENRSIEQTLDLGWKMISLLPESELVRIPPQI
                     LAKYLPRDDRDDQNDRGGHKESHEVAALKEPKEK"
     gene            1823..2455
                     /locus_tag="PLQ30_08170"
     CDS             1823..2455
                     /locus_tag="PLQ30_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPW02462.1"
                     /translation="MAEQMPAPTKTNLIKEKRNLQLALQGYDLLEKKREILVIELMKR
                     MDSLELLEREVADITEKAYAALRKMLLLVGRERALAISSMPVREITLSASRINVSGMR
                     LPSLDVHASSPTLNYSFMNSFAICDETVIEFTELLDKLSTAAGMRSIVWRLAREVRKT
                     QRRVNALDKMVIPRSQSIVKYIDSSLDERERESLFAVKMLKKRQIGQQES"
     gene            2452..>2915
                     /locus_tag="PLQ30_08175"
     CDS             2452..>2915
                     /locus_tag="PLQ30_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPW02463.1"
                     /translation="MKRMLGTILVAINGSDASICAFKYALALAKAFGSKIIACYVVDT
                     ATIRQLALSRIFVPEESEEYERNLENSGLRYLNFCVELAHQKQMYIETSIRRGSVSGE
                     IVKYSMEIDADIIVLGGNPVDVLYRDAIADSNHEVLKNARCPVLFVKPPIGD"
BASE COUNT          766 a          664 c          756 g          729 t
ORIGIN      
        1 atatcgtgcg cctcattggc ccagatgcgc tgccagatga gcagcgactc atactgctta
       61 ctgcagatat gataagaaac ggtttccttc agcagagcag ttttgataga gtcgatatgt
      121 attgcgctcc tcagaagcag actcttttgc ttcaatgcat tctgactttc catgagcttg
      181 cggagactgc aataaagaat ggtgctcctc ttcccaaggt atccgcgctg ccaatacggg
      241 agaagattgt tcgcctcaag agttcgctcg agaatgataa ggtagaagaa ggcaggatgg
      301 taatacagga aattcaggtt gcctttgagc agcttggtgt tacggcacaa ggagcggttc
      361 tagcatgaga ggtcttgaat accacggaat agagcgcgca gatgggccga ttatcattgc
      421 tgaacgcaga gaggacatcg gtttttccga gctcgttagg gtcaaagata ggcagggaga
      481 atatcgactt ggccgggttg tggagctatc tgataaggca gtggccattc agctctttgc
      541 agagaatacc ggcatatcga tagacgatgc ctgggtcgaa tacctcgaaa agcccttgac
      601 cttccgtgtt ggcgaaggca ttgttggcag aatcttcaat ggtcttgggg agcccataga
      661 tggctatccg cctatattgt cttcagacct cagggatata aacggcctgc ccataaatcc
      721 gagcgctcgc gtgtacccca gggacttcat ccagaccgga atttcagcaa tagatggcat
      781 gaataccctt atccgtggcc aaaagttgcc gattttttcc ggcaatggcc ttcctcataa
      841 taggctcgca gcccagattg tgaggcaggc aaaagtactc agcggagaaa gtcaatttgc
      901 aatagtattt gccggtatgg gaataaaata cgatgttgcc cgattcttta tagattcttt
      961 tgagagctct ggcgtcttag gccgagttgt aatgtttctt tcgctcgcgg acagtccatc
     1021 gatagaacgc cttgtaacgc cgcgcacagc gctttccgcc gcggaatacc tcgcatacga
     1081 aaagaatatg catgttctgg tagtcatgac cgatatgaca aactattgtg aggctcttcg
     1141 tgaagtctcc gcagcccgca acgaggtgcc ttcaagaaag ggttatccag gctatctata
     1201 ctcagacctc gcttcgatct acgagagagc gggaagaatc gaaggcagcg aaggttccat
     1261 aactcagctt cctattctct cgatgcccaa cgacgatata tcacacccca ttccggacct
     1321 tacgggttat ataaccgaag gtcagatcgt tctagagcgg gaactttcgc agcgtggcgt
     1381 gtatccgcca atcgcggggc ttcctagcct ttcgcggctg atgaaagacg gcatagggga
     1441 gggtatgact cgagaggatc accgagaact tgctgcccag cttttttccg cctatgccta
     1501 tgttcacacg gttcgagatc tagccgcaat cataggagaa gaggagcttt cggacaggga
     1561 taaaatattc ctgaattttg gtgatcattt tgagagccag tttttagcgc aaggcgagtt
     1621 cgagaaccgc agtatagagc agacgctcga cttaggttgg aagatgatat cgctcctccc
     1681 ggaatctgag cttgttcgta tcccgcccca gattcttgca aaatacctgc cgcgtgatga
     1741 tagggatgat cagaatgatc ggggtggtca taaagaatcc catgaagtgg ctgccctaaa
     1801 agagccaaag gagaaataac ccatggctga gcaaatgcct gcgccgacca agacgaatct
     1861 catcaaggag aagcgaaatc tgcaacttgc gcttcagggc tatgatcttc tcgagaaaaa
     1921 gagggaaatt cttgtcatcg agctgatgaa acgcatggat agtctcgagc tcctggaaag
     1981 ggaagttgcg gatataacag aaaaagccta tgcagcctta cggaagatgc tccttttggt
     2041 tggtcgagag agggctcttg caatttcatc tatgccggtg cgcgaaatta ctctcagcgc
     2101 atcacgtatc aatgtcagcg ggatgcgact tccttcgctc gatgtacacg ccagtagccc
     2161 cactttgaat tattctttta tgaattcatt tgctatatgt gacgaaactg tgatagagtt
     2221 tactgagctt cttgataagc tctcgacagc ggcaggaatg agatcgattg tatggcgcct
     2281 tgcgcgggaa gtccgcaaga ctcaacgcag agtgaacgca ttggacaaga tggtgatacc
     2341 taggtcacaa tcgattgtaa aatatatcga ttcttctctg gacgagcggg aacgcgaatc
     2401 gttgtttgca gtcaaaatgc ttaaaaagcg gcaaatagga cagcaagaat catgaagaga
     2461 atgctgggga ccattttggt ggcgatcaat ggttcagatg cctccatatg tgctttcaaa
     2521 tatgcattgg cgctcgcaaa ggcctttggt tcgaagatta ttgcctgcta tgtggtcgac
     2581 acggctacta tccgtcaact tgcgctctcc agaatcttcg tacccgaaga gagtgaagaa
     2641 tacgagcgaa atctcgaaaa ttcgggcctg cgctatctaa atttttgtgt cgaacttgct
     2701 catcagaaac aaatgtatat tgagacttcc attcgcagag gctcggtttc tggagaaata
     2761 gtcaagtatt cgatggagat cgatgcagat atcatagtgc taggcggtaa cccagtcgat
     2821 gtgctctatc gcgatgcgat tgcagattcc aatcacgaag tcctaaaaaa tgcccgatgc
     2881 cctgtgctct ttgtcaagcc gcccataggg gatga
//
LOCUS       DAOMZL010000145         2890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_34124_length_2890_cov_16.489594, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000145 DAOMZL010000000
VERSION     DAOMZL010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34124_length_2890_cov_16.489594"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1052
                     /locus_tag="PLQ30_08180"
                     /pseudo
     CDS             78..1052
                     /locus_tag="PLQ30_08180"
                     /inference="COORDINATES: protein motif:HMM:NF015038.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(1076..1501)
                     /locus_tag="PLQ30_08185"
     CDS             complement(1076..1501)
                     /locus_tag="PLQ30_08185"
                     /inference="COORDINATES: protein motif:HMM:NF014599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HPW02464.1"
                     /translation="MDKNKKPGIKIDSIILAEISFKRSPKIPDSPTFDFRIDTKSLIS
                     DDKKTLQETVSVAVESSPDKSISASCVMVGQFTCECDETNMTLDEFSKANAPAIIYPF
                     CREALASVSVQSGMPPIILPVVNFQLIANRSKETTENNK"
     gene            complement(1501..1908)
                     /locus_tag="PLQ30_08190"
     CDS             complement(1501..1908)
                     /locus_tag="PLQ30_08190"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW02465.1"
                     /translation="MDKLDELRKISENSHNDKENQRYVLRYVVTAEILNIMEKNDIRK
                     ATLAKQLGYSKGHISRVLSGDRNMTLDTISDIAMALGVNIEFTLKEKKATQKHTEQDA
                     FFWLISNDKSEYNRQECGDDFVYGNPKIVPWVA"
     gene            complement(1901..2290)
                     /locus_tag="PLQ30_08195"
     CDS             complement(1901..2290)
                     /locus_tag="PLQ30_08195"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPW02466.1"
                     /translation="MLTRVLYGDGKNFWFVIDEGEERKSQIYKFIVALTPDEWKKIVR
                     VLDAINTKPMTYMHQEKFKQLEGKIWEIKQREIRIACYWQIRGELLVGVYGLRKKANK
                     WSKNEIDNARGWYNFYKEKDFRGAING"
BASE COUNT          801 a          618 c          575 g          896 t
ORIGIN      
        1 cataatgcaa ctttgtgcat caccaaatcg catataaata ggatacatcg agtttccata
       61 gctgtcaaca aaaaacaatg gggttcatca agaagtatgg gccctgtgga tgcaaagact
      121 gtcagagtcc gattatacaa gcgcctgggc cggtgaagtt cattcctggt tcacggtttt
      181 ccaataacac tcttgccttt tttctcacct cgaagtttgt ggatgcgcag cccttctatc
      241 gtatggagca aatcttgagt cgctttggga ttgatacacc gcgggcaacg ctctgtgcct
      301 tagcgattcg agcggggaga agcttaggcg atctcataga ggcgatgaag gaggatatca
      361 agcgttctcc tgtcgtactc atggatgaaa cggtggtgca ggtgctgcat gagagaaact
      421 cgagtccgca atcgaagcac ttcatgtggg tgactgaagg cttctctgaa ggtaagccac
      481 tcatcttctt tcactatcat acgagccgct caaaagagat tcccttaaaa tttttgaaag
      541 actaccaggg gtacctgcag actgatggat actctggcta tgatgagata gggagagttc
      601 ctgggattac ctatgtaggg tgcttagctc atgtgcggcg gaagttcatc gatgccgaga
      661 agctggggag taaggaagcg agtatcttta tctcgatgat tggagaactg tatgagaaag
      721 aagcagagct acgacaacag tatgaagaag ggcttctcac tacggaaagc tttcttagcc
      781 tccggagaaa ggagcaagcc ccgagactca cggcgatgca cgcgtggctt aggacgcatg
      841 caggcataag tcctccatag atgagctttg cgatagcggt gagctatgag ctgagtcaat
      901 gggagaagct tacccactat ctcgaccacg agctcctcac ccctgatacg aatactatcg
      961 agaatgcgat aaggcccttt gtcataggca ggaagaactg gctattttcg gatacgcctt
     1021 taggagccca tgcgagtgca ggcatctttt gacggttacg tttcaatggc aattattact
     1081 tattattttc agttgtctct ttggatcgat tagctatcaa ttggaagttg actactggaa
     1141 gaataatggg aggcatgcca gattgtaccg aaaccgatgc aagtgcttct ctacagaatg
     1201 gataaattat cgctggcgca ttagcttttg aaaattcgtc tagtgtcata ttggtttcat
     1261 cacattcaca agtaaactga ccaaccatta cacatgaagc gctaatgctt ttatcagggc
     1321 tggattcaac tgctaccgat acagtctctt ggagagtttt tttgtcatct gatattagtg
     1381 actttgtatc aatcctgaag tcaaatgtag gtgagtctgg tatttttggt gatcttttaa
     1441 aggatatctc tgcaagaatg atagagtcta tctttattcc aggcttcttg ttcttatcca
     1501 ttaggctacc catggaacta ttttaggatt tccataaaca aaatcgtccc cgcattcttg
     1561 ccgattatac tcacttttgt catttgatat cagccaaaaa aaggcatctt gttctgtatg
     1621 cttctgagtg gccttttttt ctttcagagt aaactctata tttacaccca aagccatagc
     1681 aatatcacta atggtatcaa gagtcatgtt tctatcccca ctaaggaccc gtgaaatatg
     1741 ccccttacta tatcctaatt gctttgccag agttgctttg cgaatatcat ttttctccat
     1801 aatattcaaa atctctgcag tcacaacata tcgcagtaca tacctttgat tttctttatc
     1861 attgtggcta ttctcagaaa tttttctaag ctcgtctaat ttatccattt atggcacctc
     1921 gaaaatcttt ctctttatag aaattgtacc aacctcttgc attatctatt tcattcttgg
     1981 accatttatt tgcttttttt cttaacccat atacccctac caacagctcc ccgcggatct
     2041 gccagtaaca tgcgattcgg atctcacgtt gcttaatctc ccaaatcttc ccctcaagct
     2101 gtttgaattt ctcttggtgc atatatgtca ttggcttggt attaatagca tccagaacac
     2161 gaactatctt tttccactca tctggagtta atgcaacaat aaatttataa atttgactct
     2221 tgcgttcttc accctcatcg attacgaacc aaaagttttt cccatcacca tacaaaactc
     2281 tagttaacat aaatgtaaac ccaaccgctt tgctcgtcaa ggggaaaaat gaaatatttt
     2341 ttgttggttt ctatccaaag actatacatt tgtatatata atgacatctt tctatcttat
     2401 tgctccttct atccctacat taaaacatgt tttaattatc ttaacacgaa tggattaaaa
     2461 tgcaacgaga aagcagcagt ctcattaatc gtgatgaaga ttattgagct cgaagaattg
     2521 ctgcttagct acctttgcac aaaattgtcg tgcaccccaa tgggggtatt ggatatagga
     2581 attcagataa gtgctattcg acaatccatg tatggtaatt gaagagagcc ctaggctgaa
     2641 cataccatag agcaattgct acattaaaat tagccaaatt gtctttataa tcaatttttc
     2701 aaagaagact ttttgaaact ttcagattaa agatccttat ctacaagctc aattctcgct
     2761 tttttatacc taggcaagaa ggggcactcc gaagacttca tagagttgct tttgtttttt
     2821 cgtcatcttg ccaacctttt cggtatgtcc aggatagcta tagcactcga tgacatcgag
     2881 ttcatcgaga
//
LOCUS       DAOMZL010000146         2883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_34234_length_2883_cov_15.066478, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000146 DAOMZL010000000
VERSION     DAOMZL010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34234_length_2883_cov_15.066478"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="PLQ30_08200"
     CDS             complement(<1..1361)
                     /locus_tag="PLQ30_08200"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HPW02467.1"
                     /translation="MILLKDCLRVVQLKAEDLCGVDVLVEGNRITQIAKNIAVPCGSR
                     VIDASHLVVAPGLVNTHHHFYQTLSRNLPAVQTAKLFDWLVYLYEIWKGIDEEAVYVS
                     SLLAMAELIKTGCTISTDHHYIYPQGFETDIPTLQFKAASDIGMRFLATRGSMSRSKK
                     DGGLPPDSVVQDEDTILEHCEQTIQKFHDSASDSMRRVALAPCSPFSVSEELMRESAI
                     LARKYGVRLHTHLAETSDEEDYCVQMYGRRPLRLMEDCGFLGADVWYAHGIFFNDEEL
                     ETLAKSKTGIAHCPSSNMRLGSGICRVREMLDIGVPVGLAVDGSASNDSSDMLGEIRQ
                     ALLLQRIRYGATGLTSKEAWQMATKSGAEILGWEQAGSIEVGSLADIACWNVDKLAYA
                     GSLSDPSAALLFCGYDHAAEMVIVNGKIVVDNGRIVCIDEEKLKEDANMVALRLYKKA
                     GIA"
     gene            complement(1358..2836)
                     /locus_tag="PLQ30_08205"
     CDS             complement(1358..2836)
                     /locus_tag="PLQ30_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPW02468.1"
                     /translation="MIDLVIDESQRKKNIERCKKRGILLPTFAQMRDPTKISATIKAE
                     LSRIGLWDVHPRNLFRVNWHNEPKEFGGGFGSVNYIEIPRAISGTKARIIGLAGKWFP
                     TGAHKVGAAYACLAPELVTGRFDPTTKKAVWPSTGNYCRGGAYISRLLACPSVAILPE
                     GMSRERFDWLKTMAEEVIATPGSESNVKEIFDKCLELQRTRHDVVIFNQFDQLPNHLW
                     HYAITGPAIEEVFATLNGSAVPASGACAHVCGVVLASGSAGTLGSGSYIKEKFPGAKL
                     AVAEALQCPTILENGFGAHRIEGIGDKHIPWIHNLRDTDAAVGVDDELTIRLIRLFNE
                     PAGQKLLLEVGTDPSIVEKLDLLGISGVGNLIAAIKFAKYYELGEDDIVFTMFTDSME
                     LYQSRLAELTEERGLYDQRQADRDYECLQGLSVDYVFEMTHVDKRRVHNLKYFTWIEQ
                     LGKELPELRAQWSDYRNYWSGLHEQVCALDMLIEDFNAEVLK"
BASE COUNT          703 a          745 c          713 g          722 t
ORIGIN      
        1 tatgctattc ctgccttttt gtataatcga agagcgacca tatttgcatc ttcttttagc
       61 ttctcctcgt cgatgcagac gatgcggcca ttgtcgacca ctatcttgcc attgacaata
      121 accatctctg cggcatggtc atagccgcag aacaggagtg cagccgaggg gtctgatagc
      181 gagcctgcat atgcaagttt gtcgacattc cagcaagcta tatctgccag ggatcctact
      241 tcgatggaac cagcttgctc ccaacccagg atctcagcac cgcttttagt tgccatctgc
      301 caggcttctt tggaagtcag gccggtagca ccatagcgta tgcgctgcag cagcagcgcc
      361 tgtcttatct cgccgagcat gtccgaacta tcattggaag cagagccatc cacagcaagc
      421 cccacaggta ctccgatgtc caacatctct cttacgcggc atatgccaga gcccagtctc
      481 atattggacg aggggcaatg agcaatacct gttttggatt tggcaagcgt ctctaactct
      541 tcatcattaa aaaagatacc atgagcatac caaacatcgg ctccaagaaa gccacagtct
      601 tccatgagcc taagagggcg tcggccatac atctggacac aataatcctc ttcatcggag
      661 gtttctgcca aatgagtgtg caacctcact ccatatttcc tggccaggat cgccgattct
      721 ctcatcagtt cctcggagac cgaaaaaggg ctacaaggag caagcgcaac acgccgcatc
      781 gaatcagaag ctgagtcgtg gaatttttgg atcgtctgtt cgcagtgttc taggattgta
      841 tcctcgtcct gtactacaga atcaggagga agcccaccat ccttcttcga tcgggacata
      901 gaaccgcggg tggcgagaaa gcgcatgccg atatcggatg cagccttaaa ttgaagagta
      961 ggaatatctg tttcaaaacc ctgtggatat atgtaatggt gatccgttga gattgtgcat
     1021 cccgtcttta tcaattctgc catggccaaa aggctcgaga catagaccgc ctcttcgtct
     1081 atacctttcc atatttcata gaggtaaaca agccaatcga agagcttggc ggtctgcacg
     1141 gcgggcagat tgcgcgaaag ggtctgatag aaatgatggt gtgtattgac taagcccggc
     1201 gctacaacaa gatgcgaagc gtcgataaca cgagaaccac aaggaaccgc gatgtttttt
     1261 gcaatttgag ttatccgatt tccttccacc agtacatcga cgccgcatag atcttcagct
     1321 tttagttgaa cgactcgaag gcaatccttt aagaggatca cttcagaacc tccgcattga
     1381 agtcttcgat gagcatatcg agcgcgcata cttgctcatg cagaccggac caatagttgc
     1441 gataatcgct ccactgagct cgaagctcgg gaagttcctt tccgagctgt tcgatccagg
     1501 tgaaatactt gaggttgtgc acacgccgct tgtcaacatg cgtcatctca aagacataat
     1561 cgaccgataa gccctgcagg cattcatagt cgcggtcagc ctggcgctga tcgtataaac
     1621 cccgctcctc agtcagctcc gcaagacggg actgataaag ctccatcgag tcggtgaaca
     1681 ttgtgaatac gatatcatct tcgcccagtt catagtactt tgcgaacttt atggcagcga
     1741 tgagatttcc cactccggat atgccaagca gatccaactt ttcgactatg gaaggatcag
     1801 ttcccacttc taagagaagt ttttggccag ccggttcgtt gaacagccga atgagtcgaa
     1861 ttgtaagttc atcgtcgact ccaactgctg cgtcggtgtc acgaagattg tggatccagg
     1921 gaatatgctt gtcgccaatt ccttctatac gatgtgcccc aaaaccattc tctagaattg
     1981 tggggcactg cagcgcctcc gctaccgcga gttttgcgcc ggggaatttt tccttgatat
     2041 aagagcccga cccgagtgtt cccgctgagc cggaagccag cactacaccg caaacatggg
     2101 cgcatgctcc gcttgcaggg acagccgagc cgttgagcgt agcgaacact tcttctatgg
     2161 ctgggccggt gatagcataa tgccaaagat ggttgggtag ctgatcgaat tgattgaaga
     2221 tgacaacatc atggcgagtc ctttgcagct caaggcattt gtcgaagatc tcttttacat
     2281 tagattcgct gccaggtgta gcaatgactt cttctgccat ggtcttaagc caatcgaagc
     2341 gctcgcggct catcccctca gggagaattg cgacgctcgg gcaagcgagg aggcgggaga
     2401 tgtaggcgcc gccccggcag tagttgccgg tgctcggcca cacggctttt ttggtcgtgg
     2461 gatcgaagcg tcccgtgacg agctcgggag cgagacaggc ataagccgcg ccgaccttat
     2521 gtgcacccgt agggaaccat ttgcctgcaa gtccgatgat tcgagccttg gtgccagaga
     2581 ttgctcgagg aatctcgata tagttgactg atccgaaacc acctccaaat tccttgggct
     2641 cgttgtgcca gttgactcta aagagatttc gcggatgtac atcccaaagg ccaatcctgg
     2701 agagttcggc ctttattgtg gccgaaatct ttgtgggatc tcgcatctgg gcaaaagttg
     2761 gaagcagaat tcctcttttc ttacagcgtt cgatgttctt ttttctttgg gattcgtcga
     2821 tgactagatc gatcatgccg aactccttac aatgatatta tatgggatta tcagcaagtg
     2881 ata
//
LOCUS       DAOMZL010000147         2824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_35326_length_2824_cov_17.143734, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000147 DAOMZL010000000
VERSION     DAOMZL010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35326_length_2824_cov_17.143734"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PLQ30_08210"
     CDS             complement(<1..428)
                     /locus_tag="PLQ30_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPW02469.1"
                     /translation="MKKKIVLAYSGGLDTTVIVPWLKEHYDCEVIAVCGDVGQEADFD
                     GIGARALASGASKFIKLDQKEEFVKEYLWALVKAGTPYEKKYLLGTSAARPLLAKGLT
                     EVALAEGADAMAHGCTGKGNDQVRFELGVKAFAPEMEVIAP"
     gene            725..1777
                     /locus_tag="PLQ30_08215"
     CDS             725..1777
                     /locus_tag="PLQ30_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPW02470.1"
                     /translation="MIYRRFMLLLVALLIAILPVTAFTPKREYTMQVNVTQAVDWGAG
                     AQKFADLVAQKTNGVINIKPYWSSSLLAGKQTNWYQAVADGAIDFATESTMNACTVIR
                     PFNLFSLPFFINTYDNVDKIENGKAGARMIAEMERGGVKFLAWGENGFRQITNSKREI
                     RTPKDVEGLKIRVVGSPIMIDIFKQLGADPINMNWGDAVTAFQQGVVDGQENPKGVLI
                     GNKIWEYHKYMTIWNYLIDPLVFCVNSQVWKSFPPDIQAQIQEAALEAGEWEKALARA
                     YLDGDKSLKIFKEKFKDPRDPENLFVIAPSKGMKITVLTAQEIDAFKEKTKPVYDKWI
                     PTIGEDIYKLALEDMK"
     gene            1868..2383
                     /locus_tag="PLQ30_08220"
     CDS             1868..2383
                     /locus_tag="PLQ30_08220"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPW02471.1"
                     /translation="MKKKRICIDELMGAIFLAIMAIIAFTNVINRYFFHRGFAFTEEI
                     TVNLFVWITLLGIAIAFRRGANLKMTNLFDTFSPNLKRVALIISTCLGVMIFCFLIYN
                     SAREIYKNITFYHAFSESLGIPTWIYSLGTPVFSIFVIKEIIASGIREIKSLKAPLPS
                     GNSENSKEEAQ"
     gene            2383..>2824
                     /locus_tag="PLQ30_08225"
     CDS             2383..>2824
                     /locus_tag="PLQ30_08225"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPW02472.1"
                     /translation="MAALLVFGVFIFLIIIGVPIASSLGTAAIGAIMNYDLGMSMISR
                     NFEANIAKFPLVAIPFFILAGVLMDKSKIVDGIARFLILLTGRRRGGLAIAAVIACIF
                     WGAVSGSGPATAAALGLVFITPMIKQGYNKYFAATTIAAGAGLSI"
BASE COUNT          802 a          623 c          619 g          780 t
ORIGIN      
        1 ggagcaatta cttccatctc cggagcaaag gccttgactc cgagctcgaa gcgcacttgg
       61 tcgttgcctt tgccggtaca gccatgcgcc attgcatctg cgccttcggc gagcgccact
      121 tcagtgagac ctttggcaag gagtgggcgc gctgcgctgg taccgagaag gtattttttt
      181 tcgtaaggag tacctgcctt gacgagtgcc cagaggtact ccttcacaaa ctcttctttc
      241 tgatcgagct tgataaactt cgatgctccc gaagcaaggg ctcgagcacc gatcccatcg
      301 aaatcagcct cttgcccaac atcaccacag actgctatca cctcgcagtc ataatgttct
      361 ttaagccagg gtactatcac agtagtatcc agtccaccgg aataagcaag gacgattttc
      421 tttttcataa gtccatttct ccattgaatg aatatgtaaa tcacaacaga gcataaagcc
      481 cttgagctct attttctgag ggtgtctaat ccatatattg tctatctttg agccgaatca
      541 aaaatgcaca gtgtgcataa gttcattttc ttggaaatat tataaaacga aatagaattt
      601 tatgcaatat actatgcatg aatatgtcat ctattccgaa ggatactaaa tatacagaat
      661 tcttgaataa ttgaacattg acagaataaa aattgggagc gaaaattgcc atcaggaggt
      721 caatatgatc tatcgtagat ttatgctttt actcgtggcg cttcttattg caatcctgcc
      781 agtcacagca tttactccca aacgagagta tacaatgcag gtaaatgtaa cccaagctgt
      841 agattggggt gccggcgcac aaaagttcgc cgatctggtt gcccaaaaga caaacggagt
      901 aataaatatc aagccatatt ggagttcgtc gcttctggct ggcaaacaga ccaactggta
      961 tcaagcagtt gcggatggag ccatagattt tgccaccgaa tcgacgatga atgcctgcac
     1021 tgtaattcgt cctttcaatc tattcagcct gcctttcttc atcaatactt acgataatgt
     1081 cgacaagatc gagaatggca aagctggcgc tcgcatgatt gctgaaatgg aaaggggcgg
     1141 ggtcaagttc cttgcatggg gagaaaatgg tttccgccag ataacaaatt ccaagcgcga
     1201 aataaggaca ccaaaggacg tcgaaggcct caagattcgt gttgtcggat cgccgattat
     1261 gatcgatatc ttcaagcagc tcggcgcaga tccaatcaat atgaactggg gagatgcagt
     1321 tactgccttc cagcaaggtg ttgtcgatgg tcaggaaaat ccaaagggtg ttttaattgg
     1381 caataagata tgggaatacc ataagtatat gaccatttgg aattatctta tagatccgct
     1441 tgtcttctgc gtaaattccc aggtatggaa gtctttccca cccgatattc aagcgcagat
     1501 acaagaagcg gctttggaag ctggagagtg ggagaaagca cttgctcgcg cttatctcga
     1561 tggagacaaa tcacttaaga ttttcaagga aaaattcaaa gatcccaggg atccggaaaa
     1621 tctgtttgtt atagctccct ctaagggaat gaagatcacc gttcttaccg ctcaagagat
     1681 agacgcattc aaagaaaaaa caaaacctgt atatgataag tggataccca caatcggcga
     1741 agatatctat aaactcgcgc tcgaagatat gaaataacga gccttacgcc gcaacataca
     1801 ggtaccgtat agtaccttct attcttggcc ggcctctatg gccggctttt ctctaaggag
     1861 aaccataatg aagaagaaaa ggatatgcat cgatgaactt atgggcgcta tttttctagc
     1921 gattatggcg atcatagctt tcaccaatgt tatcaaccga tatttctttc atagaggttt
     1981 tgcatttaca gaggaaatca ctgtcaatct ctttgtatgg atcacgctct taggaatcgc
     2041 aattgccttt cggcgcggtg caaatctaaa gatgaccaac ttatttgaca cattctcgcc
     2101 caatctaaag cgtgtggcat tgataatatc cacatgcctc ggagtaatga tattttgttt
     2161 tctgatttat aattctgcgc gagaaatcta caaaaatata actttctatc acgcattttc
     2221 ggaatctctt ggtattccaa catggatata tagccttggg acgcctgtgt tttcgatatt
     2281 tgtcataaaa gaaatcatcg caagcggtat tagagaaata aaatccctca aagcccctct
     2341 tccttcaggc aattcagaaa attccaaaga ggaggctcaa taatggcagc gcttttagta
     2401 ttcggagttt ttatcttcct tattattata ggggttccga tcgcttcatc gcttggtaca
     2461 gcggccatcg gagcaattat gaactatgat cttggaatgt ccatgatatc tcggaatttc
     2521 gaggccaata tcgccaaatt ccctcttgtc gcaatcccat tttttatact tgccggggtg
     2581 ctcatggaca aatcaaaaat agtagatggc attgcacgct ttttgatatt gctcacaggg
     2641 agacggcgcg gcggattagc aattgccgcg gtaatagcct gcattttttg gggagctgtc
     2701 tcgggctcgg ggccagcgac cgccgctgcc cttggacttg tatttataac tcccatgata
     2761 aagcagggct acaataaata ttttgcagca actacgatcg ctgcgggtgc ggggctttcg
     2821 atca
//
LOCUS       DAOMZL010000148         2772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_36315_length_2772_cov_16.597718, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000148 DAOMZL010000000
VERSION     DAOMZL010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36315_length_2772_cov_16.597718"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            539..1432
                     /locus_tag="PLQ30_08230"
     CDS             539..1432
                     /locus_tag="PLQ30_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPW02473.1"
                     /translation="MNRVSKKLLVFAALLLLLGGEVIAQQKLVIGFSVSTQVNPFYVA
                     MADGVKEEAARQGVEVYVLNANDKLDKQIADVEDLIQKKIQVLLINATHDGAVAVVEK
                     AAKAGIPVITLQREIPTSMVISHIGTDNVVIGRMGAQWIVDKLGGKGNVVVMEGIPGA
                     DSSESRKIGSAEVFKKYQGIKIVAQQSGQYDRAKAMNVMENILQAQPKIDAVYCFNDE
                     MAMGVLAAVKAAGRTGILITGMDANQDAVEAVKKGELAMTIALPPKDIGRLGVVYAKW
                     LKTGEISIPVKSVMPVQFITP"
     gene            1519..>2772
                     /locus_tag="PLQ30_08235"
     CDS             1519..>2772
                     /locus_tag="PLQ30_08235"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPW02474.1"
                     /translation="METQKSEYLLSMRGIVKDFPGVRALDSVDFNLRHGEIHGLVGEN
                     GAGKSTLLKILSGALRADSGDILLEGNPVSINNPRQSRELGIVVIYQELSLIPYLDVA
                     RNIFLGHEAEVGSHFVLHRQKMCQEASKLLKEFCSDLDPQSLVAELSVAEKQLVEIAR
                     ALSQQSKILLMDEPSAPLSEREVEQLFNAIRTLKKQGVTIVYVSHRLEELAKITDRIS
                     ILRDGRMVETRDTQSIDIEEIIRLMVGREISEHYPKEHAIRGERLLEVSNIAEHGEIP
                     LQINAGEVVGLAGLVGAGRTELARSIFGANPLRRQRLSFLGKSIQPNSPAEAINYGIG
                     MVPEDRKEQGLILQMTIRDNLTIPMLSELFNGLSINRHRQREIAQAQVDQLKIHITSL
                     EQQALLLSGGNQQKVVLGKWLAKNCR"
BASE COUNT          838 a          496 c          679 g          759 t
ORIGIN      
        1 tatcgttatt aaagcgcctt ctattattgg aattattttc ttcatgaccg cattggcctc
       61 caaatatcaa ttatatcaag cgagagaata tatttccatc ttcttcagat ctgccttgct
      121 ttaatcatgg ctcactttac tacaaactag gatcatttca gctgccagtt tattttacaa
      181 ggtttgcaag taatataagc acaatatcag aactatgaag agcttcattg cagaactctc
      241 tattcttgag gttgagaaga tgaagatcat cttgggcact gtgatcaggt taggataaat
      301 taagacctca gtctttggca cattctttca cttgggtctg tatttcttac atgatcgtag
      361 atttgcatag ctaagtctgt ctaattctta aatgacaaat gagaataaat tgttgacgat
      421 agtgcaaata tattgtataa tatacaacaa attgatgctg acttaagaat tcattagaac
      481 attaagaaca gtatcgacag ctgtttctaa gctgtatttc cttaagagga ggtttcatat
      541 gaacagggta tcgaaaaaac tattggtttt cgctgctttg cttttattgc tgggaggaga
      601 agtgattgca cagcaaaagc tagtaatagg cttctctgtc tctactcagg taaatccttt
      661 ctatgttgct atggctgatg gagtaaaaga agaggcagct cggcagggcg ttgaggtata
      721 tgtgcttaat gctaacgata agttggataa gcagattgcc gatgtagaag acttgattca
      781 gaagaaaatc caagtacttc tgattaatgc tacacatgat ggcgcagtgg ctgtcgtaga
      841 aaaggctgcc aaggctggaa ttccagtcat tacccttcag cgagagattc ccactagcat
      901 ggtgatctcc catattggga ctgacaacgt tgtgattgga agaatgggtg cacaatggat
      961 tgtggacaag cttggaggaa agggcaatgt agttgtgatg gaaggaattc ccggagcaga
     1021 ctcttcagag agccgaaaga tcggatcagc tgaggtcttc aaaaaatatc aaggaatcaa
     1081 gatcgtagcc cagcaaagcg ggcaatatga cagagctaag gcaatgaatg ttatggagaa
     1141 catccttcaa gcacaaccta agattgatgc agtatattgc ttcaatgatg agatggctat
     1201 gggcgttttg gcggctgtaa aggcagcggg acgaactggg attctgatta cgggtatgga
     1261 tgcaaaccaa gatgcagtgg aggcagtaaa aaaaggcgag ttggcaatga caatagctct
     1321 acctccaaag gatattggtc gcttaggcgt ggtatatgcc aaatggctga agactggaga
     1381 aatatcgatt ccagtaaagt cggttatgcc agtacagttc attacgccat aagagaatag
     1441 ttgcaacata tagatgtgag cagcaggcta tcttagcccg aatggaaatt aacagcaaaa
     1501 agcgcaggtt agtaataaat ggagacacag aaaagcgaat atttgctttc tatgcgaggt
     1561 atcgttaagg attttccagg ggtacgcgca ttggactccg ttgatttcaa cctgcgccac
     1621 ggagaaatac atggcttagt aggagagaat ggggctggca aatccactct tctgaagatt
     1681 ctaagtggtg cgttgagggc agattctgga gatatcttgc tcgaagggaa tccagtatct
     1741 atcaataatc cacggcaaag tcgcgagctg ggtatagtcg tgatttacca agagcttagc
     1801 ttgatacctt acctggatgt agcgaggaat atatttctag gtcatgaagc cgaagttggg
     1861 tcgcatttcg tacttcatcg ccaaaagatg tgtcaggaag cctctaaact actgaaagaa
     1921 ttttgcagcg atctagatcc tcagagcctc gttgctgaat tatctgtagc agagaaacaa
     1981 ctagtcgaga ttgctagagc actatcccaa caatccaaga tacttctgat ggatgaaccc
     2041 agtgctccgc tttcagagag ggaagttgag caattgttca atgcaatccg tacgctaaag
     2101 aagcaaggag taacaattgt ctatgtgtcg caccgattgg aggagctagc taaaatcact
     2161 gatcggattt ccatattgcg tgatggtagg atggttgaaa ctcgagatac tcaatcaatc
     2221 gatatagaag agattattcg cttaatggta ggcagagaaa tttcagaaca ttatccaaaa
     2281 gagcatgcta ttcgaggaga gcgccttttg gaggtaagca atattgctga gcatggagag
     2341 attccgctgc aaataaatgc tggagaggtt gtaggattgg ctggcttagt cggagctggg
     2401 cgaaccgagt tggcaagatc tatctttggg gcaaaccctc tgcgaaggca gcgtttaagt
     2461 tttttaggca agagtataca acctaatagt cctgcagagg ctatcaacta tggtatcggt
     2521 atggttcccg aagatcgtaa ggaacaaggt ctaatccttc agatgacaat tcgggacaac
     2581 ttaactattc ctatgctatc ggaacttttt aatggtttga gtattaatcg ccatcgtcag
     2641 agagaaattg cgcaggctca agtagaccag ctcaaaattc atattacatc tctagaacag
     2701 caagctctgt tattgagtgg aggtaatcag cagaaagttg ttttgggtaa gtggctggcg
     2761 aagaattgtc gc
//
LOCUS       DAOMZL010000149         2766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_36446_length_2766_cov_13.369974, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000149 DAOMZL010000000
VERSION     DAOMZL010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36446_length_2766_cov_13.369974"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..173
                     /locus_tag="PLQ30_08240"
     CDS             18..173
                     /locus_tag="PLQ30_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW02475.1"
                     /translation="MHAAKSLLEQGDLSVKEVAYRLGFEDQYHFSRLFKKKTGIAPSQ
                     WRTFMYE"
     gene            complement(329..1288)
                     /locus_tag="PLQ30_08245"
     CDS             complement(329..1288)
                     /locus_tag="PLQ30_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02476.1"
                     /translation="MKKILVVFAVLLAIFLVGCPNAPSNNVPEEKASSLKASGATTVP
                     TTEEEIIDVFASAEDMDSDPLVVDLKEFFDSFGNGSKAINRGKSTTTAKGIVKKAFSV
                     AKDLEETLIPQISDIQAALEAFPNTKELDETINVSGEAIGTYLTFTKGEATLKANAKT
                     TDNAAIAEDLSNLKEISGDASLKIEINPTNALYDIGSAIKDVKFKTNAAGDAKIVSTS
                     EGNAELPKITLNYTESLSIGLSFSKNEKGGKFILTENAKHSGDIDASALEGDPSTVVN
                     NLITEITISLKVYDDNNQVKFSKTYSSIDDFINAFWSEEEETE"
     gene            complement(1421..>2766)
                     /locus_tag="PLQ30_08250"
     CDS             complement(1421..>2766)
                     /locus_tag="PLQ30_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPW02477.1"
                     /translation="DQFKREADILRTLNHPRIPKYIESFETVIEGKFLFVLVMEYIQG
                     DTIEAFISSGKKFTEEQIEELLAELAEILAYLGSLRPPIVHRDINPRNLIIRPDGHIV
                     LVDFSGVQDAIRTVLYPGATLVGTAGYIPMEQVSGRATHRSDLYGAAATTVFMLTGRN
                     PSELPMRNLKIDLSGLLYMSPRLSYVLDNWLDPDPDRRTLSAVKASGILREKADIPSE
                     IRRDLGSEKKSEYIENSQNSSGAFWGGTYSRDSILGEILHNLEDKSEQRAYPSELPSN
                     SQLKIQDLEPGLYIKIPKRSNTSMRGATAFPVAWIGFVFFWTTMTLRMHASFFFSLFS
                     IPFWIAGFSMIKSFIGPSTTETELFLTSDGLLRKSSGFLSNSAQQCLLSDIQEAKVHK
                     SSVRINKRYLKELAIKAKGGNITFGLGLSDPELYYLEKRINEELILLKQLPTGES"
BASE COUNT          741 a          594 c          588 g          843 t
ORIGIN      
        1 attatatcca cataaagata cacgcggcaa agagtctact cgaacaaggc gatctttcgg
       61 tcaaggaagt tgcctatcga ctcggcttcg aagaccagta tcatttttcc aggctcttca
      121 agaaaaagac agggattgct ccttcgcagt ggcgaacttt tatgtatgag tgaggcgtag
      181 ccgatttttc tattctgcat tttgatgcgg ctaagatatg cgttgcgaat gttgagttat
      241 gaaaaattaa ttccaaaaag aaccgcccct gcggatggct agctgcaagg ggcgattatc
      301 tattggcaaa acccgtactt tttattcttt attctgtttc ttcttcttca gaccagaaag
      361 cattaatgaa atcatcgatg ctactatagg ttttggaaaa tttgacttga ttgttgtcat
      421 catagacctt gagactgatg gtgatttctg taatcaggtt atttactacc gtagaaggat
      481 cgccttccag agcagacgca tcgatgtctc cggaatgttt tgcattttct gtaagaataa
      541 acttgccacc cttttcgttt ttgctgaatg ataacccaat actaaggctt tcggtatagt
      601 ttaaggttat ttttggcagc tcagcattgc cttcagaagt cgagacgatt ttcgcatcgc
      661 cagcagcatt agtcttgaat tttacatctt ttatggccga gcctatatca tagagcgcgt
      721 tagttggatt tatttcgatc tttaatgatg catctccaga aatctctttc aaattgctca
      781 aatcctcagc aatcgcggca ttgtcagttg ttttagcatt cgcttttaga gtagcttctc
      841 ccttggtaaa ggtcagataa gtacctatcg cttctccact gacatttatc gtctcatcga
      901 gctcttttgt attggggaag gcctcgagag cagcttgaat atcactgatt tgtggtataa
      961 gggtttcttc tagatcctta gcgacagaaa aagctttttt gacaattccc ttagctgttg
     1021 ttgtactttt ccctctattt atcgcttttg agccattgcc aaaggagtca aagaattcct
     1081 taaggtctac taccaaagga tcactatcca tatcttcggc actagcaaag acatcgatga
     1141 tttcctcctc agtggtaggt actgtggttg caccgctagc ctttaagctg ctcgcttttt
     1201 cctctggcac attattggac ggagcattgg gacatccgac aagaaaaatg gctagcaata
     1261 ctgcaaaaac gaccagaatc tttttcattt tttcctccta tcatattctt ctaagataat
     1321 aaactatatt taattctagc gcaaaaaaat cataagtcaa gaacaatttt ctgggctagc
     1381 ttcgatcaga aaaaagagct tataaagagc ctatacagac ttaactttct cctgttggca
     1441 gctgcttaag gaggataagc tcttcattga tacgcttttc caggtagtat agctcaggat
     1501 cggagagtcc aagaccaaaa gtaatattgc caccctttgc cttgattgcg agttccttga
     1561 gatatcgctt attgatcctc acagagcttt tatgcacctt tgcttcctga atatcagaga
     1621 gaaggcattg ctgtgctgaa tttgataaaa atccagagct ctttcgaagc aagccatccg
     1681 atgtcaaaaa gagctcggtc tcggttgtcg aaggccctat gaaagacttt atcatcgaaa
     1741 aacctgcaat ccaaaaaggt atgctgaaga gagaaaagaa aaaagacgca tgcatgcgca
     1801 aggtcatagt ggtccagaaa aaaacaaaac ctatccatgc aacaggaaag gccgttgctc
     1861 ctcgcatgct ggtattactt ctttttggta tttttatata gagcccgggt tcgagatcct
     1921 gaatcttaag ttggctattc gaaggcaatt ccgaagggta ggctctttgt tcgcttttat
     1981 cttcgagatt gtgcagaatt tctcctaaaa tggaatcgcg agaataagtt cctccccaaa
     2041 aagctcccga actattttgg ctgttttcaa tgtattccga tttcttttct gaacctaaat
     2101 cccggcgaat ttccgagggg atgtccgctt tctctctcaa tattcccgag gccttgactg
     2161 cagagagagt tctgcggtct ggatccgggt cgagccagtt gtccaataca taagagagcc
     2221 tcggcgacat atacagaagt ccggaaagat ctatttttaa gtttcgcatc ggcaattcag
     2281 aaggatttcg ccctgtaagc atgaaaaccg ttgtagcagc agcaccatat aggtcagaac
     2341 ggtgcgttgc tcggcccgat acctgctcca ttgggatata accagcagta ccaaccagtg
     2401 ttgcgccagg atatagaact gtgcgaatag catcttggac tcctgaaaaa tcgacgagta
     2461 ctatatgtcc atcaggcctg attataagat ttctaggatt gatgtcccga tggacgatag
     2521 gcgggcgcag gctccccaag tatgccagta tttctgcaag ctcagcgagc aattcttcga
     2581 tttgctcttc ggtgaatttc ttgccagatg agataaatgc ttcgattgta tcaccttgga
     2641 tgtattccat tacaagcacg aacaggaatt ttccttctat cactgtctcg aagctctcta
     2701 tgtatttggg gattctcgga tgattgaggg tgcggaggat atcggcttcg cgtttgaact
     2761 gatcta
//
LOCUS       DAOMZL010000150         2736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_37056_length_2736_cov_18.017904, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000150 DAOMZL010000000
VERSION     DAOMZL010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37056_length_2736_cov_18.017904"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PLQ30_08255"
     CDS             complement(<1..620)
                     /locus_tag="PLQ30_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02478.1"
                     /translation="MRVIEQFVQSKTGIQESCEDGYVITDCFAAVIDGVTNKNAVQLG
                     LDAPGKIAMELIKKGIGDLSPILSAHDAFKAINELIYDSYCNLGLVEAMKKNPSQRFS
                     ASCVIYNSVRRELWFLGDCMALANGTIYQFSKESDRLLSDLRSFLIYIQLSQGITEEQ
                     LLEHDLCRQQIHSFLVSQTFLQNSSQNCPFAYYVLDGISEEAPRHAV"
     gene            699..1445
                     /locus_tag="PLQ30_08260"
     CDS             699..1445
                     /locus_tag="PLQ30_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HPW02479.1"
                     /translation="MRICIFENALDLGSHSAMVVSMHIRRALQEKGECSIILATGTSQ
                     LTMLDALVKDHDIDWTKVEVFHLDEYVGIPETHKASFRKYLRERFADRVPGLRSFHYI
                     KADADDLSKEIARLSDLISSKNIDVACIGIGENGHLAFNDPPANLDTKEPYLIVRLDE
                     ACRRQQVGEGWFDDLTEVPMQAISMSIPQILKSECIICTVPDKRKAEAVRMAVTAPID
                     PIHPCASLRMHKNCYLMLDHSAASLICPLS"
     gene            1456..2394
                     /locus_tag="PLQ30_08265"
     CDS             1456..2394
                     /locus_tag="PLQ30_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPW02480.1"
                     /translation="MITNNMNSYGTLIDLQVNGYGGIDFSAQDLTVEQVVYVCQLLQS
                     KGTSRFLATLVSSPVELYERNLATIKEAMQEDEIAGRLIAGFHIEGPFISEKDGARGA
                     HDKRFICKPDKKMLKCILKAGGSLVRILTIAPELTGIEDIIAMAKDENIIVSMGHTLA
                     DASQIERAIEKGASCVTHLGNGIPLMLHRTDNTIFAFLAHTELTIMLITDGHHLPVPF
                     IRTVIRACGINNIVVISDSAPLAGMPPGSYISLGGNVILEPDGKLFIPSQGCLAGSSA
                     CIAECANYLKDYIGLSTEEIQKVTSTNPMRLLREDG"
     gene            complement(2447..>2736)
                     /locus_tag="PLQ30_08270"
     CDS             complement(2447..>2736)
                     /locus_tag="PLQ30_08270"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HPW02481.1"
                     /translation="ILKPEITFFGENLPMQALREARAEATAADLMLVLGSSLLVYPAA
                     SLPQYTLEHGGQIVIVNNMPTHLDSRAILCFDELADVFEYLEAQLAEGWLE"
BASE COUNT          770 a          590 c          619 g          757 t
ORIGIN      
        1 actgcatgtc ttggtgcttc ttcgcttatg ccatcgagaa cataataggc aaaagggcaa
       61 ttttgcgatg agttctgaag gaaagtctgg ctgactagaa agctatgtat ctgttggcga
      121 cacagatcgt gttccaaaag ctgttcttct gttattccct gagagagctg tatatagata
      181 agaaaggagc gcaaatcact aagaagccga tctgattcct tagaaaactg gtatatggtt
      241 ccattggcta aggccatgca atcccctaga aaccaaagct cacgccgcac tgaattgtag
      301 attacacaag atgcagaaaa acgctgggaa ggattcttct tcatagcttc cactaaaccc
      361 aagttgcagt aactatcata gatcaattca ttgatggcct taaaagcatc atgtgcagat
      421 agtattggcg aaagatctcc gatgcccttc tttatcagct ccatggcgat tttccctggc
      481 gcatctaagc ccaactgcac ggcattctta ttggttactc catctattac tgccgcgaaa
      541 caatcggtta tgacatatcc atcctcgcaa ctttcctgta ttccggtttt tgattggaca
      601 aattgttcta ttactcgcat ctttagttcc ttatattcct tccttctgca tttatagtaa
      661 attacaataa cagaacaaaa ctcaggagga aagtgctaat gagaatctgt atctttgaaa
      721 atgctcttga tctgggtagt cattcagcga tggttgtctc catgcatatt cgcagggctc
      781 tgcaagaaaa gggagagtgt tctatcattc tagcaactgg cacaagccaa ttaaccatgc
      841 ttgatgccct tgtaaaggat catgatatcg attggaccaa ggtagaggtt tttcatctgg
      901 atgagtatgt aggcataccc gagacacaca aggcaagttt ccgtaagtat ttgcgagaga
      961 gatttgcgga tagagtgcct ggattgcgat ctttccacta cattaaggcc gatgcagatg
     1021 atttgtcaaa agaaatagct agactttctg atttaataag ctcaaagaac atcgatgtcg
     1081 cgtgcattgg cattggagaa aatgggcatc ttgcatttaa cgatcctcca gcaaatctcg
     1141 atacgaaaga gccttatctg atagtccggc tagatgaagc atgcaggcgg caacaggttg
     1201 gagaaggatg gttcgatgat ttaacagagg tccccatgca agctatttcg atgagcattc
     1261 cccagatact caagagtgaa tgcataatct gcacagttcc ggataaaaga aaggccgaag
     1321 ctgtgcgtat ggcggtaacc gctccgatcg atccaataca tccctgtgca tcgctcagaa
     1381 tgcataagaa ttgctatttg atgctagatc attctgcagc ttctcttatt tgtcccttgt
     1441 cttgagcata tcgttatgat caccaataac atgaattcct atggtaccct aatagatctg
     1501 caggtcaatg gatatggagg aatcgatttt agcgcacaag atcttactgt cgaacaggtt
     1561 gtctatgtct gccaacttct tcaatcaaaa ggaactagcc gatttcttgc cactctcgtg
     1621 agttctcccg tcgaactgta tgaaaggaat ttggcaacta tcaaagaagc aatgcaagaa
     1681 gatgagattg caggacgtct cattgcagga ttccatatcg aaggaccctt tatctctgaa
     1741 aaagacggtg ccaggggtgc gcacgataag agatttatat gtaaacctga caagaaaatg
     1801 cttaaatgca ttctgaaagc tgggggttct ctcgttcgaa tactaactat agctcctgag
     1861 ttgacaggca tagaggatat tattgctatg gcgaaagatg aaaacatcat cgtatccatg
     1921 gggcatacct tggctgatgc tagccagatc gagagagcta tcgagaaggg agcttcatgt
     1981 gtaacgcacc tgggtaatgg gattcctctt atgcttcata ggactgacaa tactattttt
     2041 gcctttcttg cacataccga gttgacaatt atgctcataa cggacggcca tcaccttcct
     2101 gttcccttca tccgaacagt gataagagct tgtggcataa ataatatagt tgtgatcagc
     2161 gattccgccc cactcgcagg aatgcctcct ggatcctata tttccctcgg tggaaatgta
     2221 atccttgaac cggatggaaa gctttttatt ccttctcaag gatgcctcgc tggctcttcg
     2281 gcatgcatcg ctgaatgtgc aaactatctc aaagattata tcggcctttc aacagaggaa
     2341 atccaaaaag ttacaagtac taatccgatg cgtttgctta gagaagatgg ttagcacgtt
     2401 gaattaaaaa acaccatata aaatcgatgt gcaatagatt gtctctttat tccagccatc
     2461 cttcggccag ttgcgcttct aggtattcga agacatcggc aagctcatcg aagcagagaa
     2521 tggctctcga atccaggtgg gttggcatgt tgttcacgat aacgatttgg ccaccgtgct
     2581 cgagggtata ctggggcaaa gaggcagctg gatagactag caggcttgaa ccaagtacga
     2641 gcataagatc ggctgctgtc gcctcggcac gtgcttcgcg cagcgcttgc atgggcaggt
     2701 tttcaccgaa gaaagtgatc tcgggtttga gtatgc
//
LOCUS       DAOMZL010000151         2720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_37400_length_2720_cov_13.918949, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000151 DAOMZL010000000
VERSION     DAOMZL010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37400_length_2720_cov_13.918949"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..894
                     /locus_tag="PLQ30_08275"
     CDS             28..894
                     /locus_tag="PLQ30_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPW02482.1"
                     /translation="MAEFLSYFRRDRLPHIWCPGCGNGTVTGALVRAIDKLGLDKNNT
                     VIVSGIGCSSRSVGYLDFDTLHTTHGRAIAFATGVKLANPKLKVVVITGDGDCAAIGG
                     NHFIHAARRNIDITVIIINNDTYGMTNGQASPMTPRGMFGTTAPYGNIEYNFDLCELA
                     KAAGATYVARATTYHYVLLEDLIAKAIMHKGFSVVEALSQCPTYFGRKNKIGGAVETL
                     NWIKDQTINVKAAASLPQEKREGKILIGELYKSAKAEFTEQYLELIDRLREADPSYIR
                     NEGESGCELNSD"
     gene            873..1421
                     /locus_tag="PLQ30_08280"
     CDS             873..1421
                     /locus_tag="PLQ30_08280"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPW02483.1"
                     /translation="MRVEFRLSGSGGQGLLLAGIVLAEAAINEGKNAVQTQSYGPEAR
                     GGSSKAEVVISDSEIDYPKATEPDYLLALSNDAYKSYGVSMKKGIIIADESLNIDPPA
                     KVPVVRLPILETASNKLGRAVVANMVALGVLGGISGVASPETLKEAVRNRVPKGTEEL
                     NTEALEQGLLLAKQAKDQLGLI"
     gene            1469..>2720
                     /locus_tag="PLQ30_08285"
     CDS             1469..>2720
                     /locus_tag="PLQ30_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPW02484.1"
                     /translation="MTDFNKLEQDFDAWNTKVEKANEKHPERKKQFVTGSNAPINRVY
                     TPLDQKDFDYAKQLGFPGEFPFTRGVQPTMYRGRLWTMRQYAGFGTAEESNKRYKYLL
                     EQGQTGLSVAFDLPTQIGYDSDHPLSEGEVGKVGVAIDSLEDMEVLFDGIPLDRVSTS
                     MTINAPASVLLAMYIAMAEKQGVSAEKLNGTIQNDILKEYIARGTYIFPPEPSMRLIT
                     NTFEYCSKNVPNWNTISISGYHIREAGATAVQEVAFTLADGIAYVEAAIKADLDIDEF
                     APRLSFFFNSHNDLFEEVAKFRAARRIWAYIMRERFGAKNPKSWMLRFHTQTAGCTLT
                     AQQPDNNIIRVTIQALAAVLGGTQSLHTNSRDEALALPTEEAVRIALRTQQIIAYESG
                     VAETVDPLAGSYYIESLTDRIEKEA"
BASE COUNT          760 a          622 c          695 g          643 t
ORIGIN      
        1 ctttgcagcg atcaaggagg cactctaatg gctgaatttc tctcttactt ccgtcgagat
       61 cgacttccac atatttggtg ccctggttgc ggaaatggca ctgttaccgg tgcccttgtg
      121 cgtgcgatcg ataaacttgg gctcgataag aacaatacgg ttattgtatc ggggattgga
      181 tgttcaagcc gctcggttgg ctatctcgat ttcgatacgc ttcatacaac gcacggtcgc
      241 gcaattgctt ttgctacggg cgtaaagctg gcgaatccta agctcaaggt agtggtaata
      301 actggggatg gcgattgcgc cgcaatcggc ggcaatcatt ttatccatgc cgctcgaaga
      361 aatatcgata taacagtgat tattatcaat aacgatacct atggcatgac aaatggccaa
      421 gcctctccta tgacgcctcg cgggatgttc gggacaactg ctccttatgg aaatatcgaa
      481 tacaatttcg atctgtgcga gctcgctaag gcggctggtg ccacctatgt agcaagagcg
      541 accacgtatc actatgtttt actcgaagac ctgattgcca aagcgatcat gcataaaggt
      601 ttctcggtgg ttgaggcact atcacaatgt ccgacctact ttggccgaaa gaataagatc
      661 ggtggtgccg ttgaaacact caattggatt aaggaccaga caataaatgt aaaagctgca
      721 gcctcattgc cacaagaaaa acgcgaaggc aaaatcctta ttggcgagct atataagagc
      781 gcgaaggctg agttcacaga gcaatactta gaactgatcg acagactaag ggaagccgat
      841 cccagctata tccgaaacga aggagaatca ggatgcgagt tgaattcaga ctgagcggct
      901 caggaggaca agggcttttg cttgcaggta tcgtattagc agaggcggca atcaacgaag
      961 gaaagaatgc ggttcagaca cagagttacg gaccggaagc ccgtggtggt tcgagcaagg
     1021 cggaggttgt tatctcggat agcgagatcg attatcctaa agccacagag cccgactacc
     1081 ttcttgcact atccaacgat gcatataaaa gctatggtgt ctcgatgaaa aaaggaatca
     1141 tcattgcaga tgagagcttg aacatcgatc caccggctaa ggtgccggtt gtgaggctcc
     1201 ccatacttga gactgcatct aacaaactgg gaagggcagt tgtggccaac atggttgcgc
     1261 tcggagtatt gggtggaatc tccggagtag caagtccaga gacgctaaaa gaagccgtaa
     1321 ggaatcgtgt gccaaaagga accgaagagc taaacactga ggcgctcgaa caagggctct
     1381 tgttggctaa gcaagcaaaa gatcagcttg gactcatcta atatatgaaa tccatttgac
     1441 ataaattggt gacctcgagg agggaagcat gaccgacttt aataaacttg aacaggattt
     1501 cgatgcatgg aatacaaagg ttgaaaaggc aaatgaaaag catcctgaac gcaagaagca
     1561 atttgtaaca ggatcgaatg ccccaatcaa tcgagtatat actccacttg atcaaaaaga
     1621 cttcgattat gctaagcagc tggggttccc gggcgaattc ccctttacgc ggggtgttca
     1681 accgaccatg tatagaggca gattatggac tatgcggcag tatgcgggtt ttggtaccgc
     1741 ggaagaatct aataagcgct ataagtattt gcttgagcag ggtcaaacgg ggctttctgt
     1801 ggctttcgat ttgcctactc agattggtta tgattcagat catccattgt ctgaaggaga
     1861 ggtggggaaa gtgggtgtgg ccatcgattc gttagaagac atggaagtgt tgttcgatgg
     1921 catcccgctc gacagagtct cgaccagcat gacaataaat gctcctgctt ccgtgcttct
     1981 ggcaatgtat attgcaatgg ctgagaagca gggagtcagt gccgagaagc taaatggcac
     2041 cattcagaat gatatcttga aagaatatat agccagagga acctatattt tcccgccaga
     2101 gccttccatg aggctcatca caaatacatt cgaatattgc tctaagaatg ttccaaactg
     2161 gaataccatt tctatttctg gctatcacat tcgagaagca ggcgcgacag ccgtgcagga
     2221 ggtagcattt accctggcag atggcatagc ctatgtcgaa gctgccataa aagcagattt
     2281 ggatatcgac gaatttgcgc ctcgcttgtc tttctttttc aattcgcaca atgatctttt
     2341 cgaggaagtg gctaaatttc gtgcagctcg gaggatctgg gcttacatca tgcgtgagcg
     2401 ctttggtgcc aagaatccaa aaagctggat gcttcgcttc catacccaaa ccgccggctg
     2461 caccctcacc gcccagcagc ccgacaacaa cataatccgc gtaactattc aggctttggc
     2521 cgcggtgctg ggcggtacgc aatccctcca taccaattcc cgcgacgaag cgcttgctct
     2581 tcccaccgaa gaggcggtac gaatagccct tcgtacccaa cagataatcg catacgagtc
     2641 cggcgtagcg gagaccgtgg atccactggc tggctcatac tatatagaga gcctcacaga
     2701 tcgcatagaa aaggaagccc
//
LOCUS       DAOMZL010000152         2719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_37422_length_2719_cov_17.301426, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000152 DAOMZL010000000
VERSION     DAOMZL010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37422_length_2719_cov_17.301426"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..384
                     /locus_tag="PLQ30_08290"
     tRNA            302..384
                     /locus_tag="PLQ30_08290"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:335..337,aa:Leu,seq:caa)
     gene            657..1127
                     /locus_tag="PLQ30_08295"
     CDS             657..1127
                     /locus_tag="PLQ30_08295"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPW02485.1"
                     /translation="MNKLKTFYTIAKYRNFTKAGELLGLTQPAVSLQMKALEEEYCTK
                     LFERIGRDVFLTEAGKVLFEYAERILTTLEEANLAIKECEDPYKGKVSFGVSMFAGAY
                     IIPPILGRYKEHHPKTMFFVKVRYAKDIFPLIIENEVDFGLMGEGEQKNTIIAF"
     gene            1163..1573
                     /locus_tag="PLQ30_08300"
     CDS             1163..1573
                     /locus_tag="PLQ30_08300"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HPW02486.1"
                     /translation="MPKDHKFADRKSVSIHDIVEEKLVLPERLSASRRYIESEFQRND
                     MSVKPYLEIRNIEVIKKLVEQNLGSNILSWASVREEVAAHRICAARITGVSFERDVLL
                     VKKREKTLFPATTLFIEFLLDEVTRNKDQFVFTV"
     gene            complement(1563..>2719)
                     /locus_tag="PLQ30_08305"
     CDS             complement(1563..>2719)
                     /locus_tag="PLQ30_08305"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW02487.1"
                     /translation="KTVRGGVIGELPSADIPNLDFILVTHAHQDHTAMLPLLVEKGIQ
                     VAIYATEPTKEFSITYCKSWLEGYKNFGIEPSYRAESIEKLKDMFVTVPYYSEFSPGK
                     NTHVTFSPSGHLAGSAILYIKDEYRIAHFGDTNFGDTLNPEPYLDFEADIGIINGSYG
                     DRVLAQPELERKFIDYVCSSKNNLLIPAAALGRGQEICLLLLDTRSKIDKDILVAKSI
                     FDNAEKLLNYKEFLRDGAEERISALLASDKLMVMANNEMEYYLRKGSIFVTPDAMLSN
                     GKSVDILDRIKDNPDDMVILSGYLAPGTVGRRLAEGRLSINAKVVLSELKVHTDLADN
                     ERILQKSLSRAKLVLVHHREEPKSSQLAGALRQKFHIEVISPHFGDRIIL"
BASE COUNT          782 a          597 c          577 g          763 t
ORIGIN      
        1 tccctttgac tcttcggacc aactaaacag tcctccctct ctgtgttcag ccctacggcg
       61 gctgcgttgg catctgtatc cttcgcatat ttggtaatta tatcctattt actgtgtagg
      121 gtaaaggcag cactatggta agaccatgtt ctgagataat gttgaatgaa ttgattactg
      181 tgcacaaaac cgtcgagcac catattaatt gaccatgata gatcatgtca catgttctgg
      241 ttacaaaaca gacttgactc tcaaagccat cctacggcaa aatgcaccat attctacatc
      301 tgccggcatg gtgaaatggc atacacggta gactcaaaat ctgctgcttg caagagcgtg
      361 tgggttcaag tcccactgcc ggcattaaaa gcgctttttg ggcgctttga ttatctttat
      421 aagcccagcg aattttcaat tatagccttc ataatcagaa aaattcctat tttaccaatt
      481 ttgtggcgat ttgattcctg tattatatgt attcaaaatc gataagacta ttaaaaaaga
      541 aacgcaaagg ctagagcact gccacatgct gttttcccct ggattctcta tgaaacacac
      601 ggcaggtgat atatactgaa agtggtctat gacgaaatcc tggccaggta aacccgatga
      661 acaaactgaa aactttttat accatcgcaa aataccgtaa ctttactaaa gcaggagagt
      721 tgctcggcct aacacaacct gcagtaagcc ttcaaatgaa agctctcgaa gaagagtact
      781 gtacgaaatt attcgaacga atcgggagag atgtttttct taccgaagca ggtaaagttc
      841 tttttgaata tgccgaaaga atccttacga cactggagga ggccaacctt gcaatcaaag
      901 aatgtgaaga tccatataaa ggcaaggtga gtttcggcgt aagcatgttt gccggtgcat
      961 atattattcc tccaattctg ggaaggtaca aagaacatca tcccaaaacg atgtttttcg
     1021 tgaaagtacg gtatgcaaaa gatattttcc ctctgatcat cgaaaacgag gtcgattttg
     1081 ggcttatggg ggaaggagaa caaaaaaata cgataattgc tttttaacag aagtaattct
     1141 gagggaagag aaagtctttg tgatgcccaa ggatcataaa tttgcagata gaaagagcgt
     1201 atccattcat gacatagtcg aggaaaagct tgttttgcca gagcggcttt cggcctctcg
     1261 cagatacatc gagagcgaat tccagcgaaa cgatatgtca gtaaagccat atctcgagat
     1321 aaggaatatt gaagtcatca aaaagcttgt tgaacagaac ttaggttcca acatactctc
     1381 gtgggcatcc gtgcgcgaag aagtagccgc tcatagaatc tgtgcagcca gaatcacagg
     1441 cgtctcattt gaacgcgatg tactgcttgt aaagaaaaga gagaaaacac ttttccccgc
     1501 taccactctc tttattgaat ttctgctcga tgaagtaacc cgaaacaaag atcaatttgt
     1561 atttacagta taattctatc cccaaaatga gggcttatta cctcgatatg aaacttttgc
     1621 ctcaaggctc ccgcgagctg agaagatttt ggttcttctc tatgatgaac caggacaagc
     1681 tttgcacgtg aaagtgattt ttgcaggatt cgttcgttgt ccgcgagatc agtatggact
     1741 tttaattcgg acaacacgac ttttgcattg atagacaatc ggccctcggc gagtcgtctc
     1801 cccaccgtcc caggcgcaag ataaccagag agaatcacca tatcgtccgg gttatctttt
     1861 attctatcaa ggatatcgac agactttcca tttgagagca ttgcatctgg ggtgacaaaa
     1921 attgaaccct tccggaggta atactccatt tcgttgttcg ccataaccat gagtttatct
     1981 gaagcaagta gtgcagatat tcgctcttca gcgccatccc tcaaaaactc cttataattc
     2041 agtagttttt ctgcattgtc aaaaatggat tttgctacaa ggatatcttt gtcgattttg
     2101 cttctagtat cgagcagtaa caaacatatt tcctggcccc tgccaagagc cgctgcagga
     2161 atcaacagat tgttttttga cgaacacaca tagtcaatga attttctttc aagctcgggc
     2221 tgggccagca ccctgtctcc gtatgatcca ttgatgatcc cgatatccgc ttcgaaatcc
     2281 agatatggct ccggattgag tgtatcgccg aagttggtat ctccgaagtg cgcaatgcgg
     2341 tattcgtcct tgatatatag aatcgccgag cctgcaagat ggccgctcgg ggaaaaggtg
     2401 acatgagtat tcttgcctgg gctgaattca gagtaatatg gcacggtcac aaacatatcc
     2461 ttgagctttt ctatgctttc tgcgcgatag gacggctcaa tgccaaaatt tttgtatccc
     2521 tccagccagc ttttgcagta agtaatcgag aactcttttg ttggttcagt cgcatagatc
     2581 gcaacctgta tgcccttttc aaccagaagc ggaagcattg cggtatgatc ctggtgtgca
     2641 tgcgtaacca gaataaaatc caaattcgga atgtccgccg aaggcaattc gccgattact
     2701 cctcctctga ccgtttttt
//
LOCUS       DAOMZL010000153         2619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_39656_length_2619_cov_14.879485, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000153 DAOMZL010000000
VERSION     DAOMZL010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39656_length_2619_cov_14.879485"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..>2619
                     /locus_tag="PLQ30_08310"
     CDS             51..>2619
                     /locus_tag="PLQ30_08310"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW02488.1"
                     /translation="MSKIMKTTALPALFERIAGEYTTKRSIFEIPESTWLDLFEQEAE
                     SAGMPIMASTISIPLGPAAGPHTQIAPNLIAAYLSGARVFELKTVQENDHLDIDKPCI
                     DALDEGYNVEWSTELSLEEARIEYINAWLVINLFARIWSHKPSDFLFNMSVGYTLEGL
                     KSAKMEAFIEGMRRPETGSYWERALEELKSFVESPLFMQAFGSQALEKARSIAEHMPV
                     RPVHSVTLSTMHGCPPEEIERIGRYLIEEKGFDTYIKLNPTLLGYEDTRTILDRLGWK
                     DIVLKSESFEHDLQFDMAISLIHTLSEVAFNKGRRFGIKLSNTLANINNGDYLPGFER
                     YLSGRALFPITINLAAKLAHALPDFGARFSYCGGVSAFNAADLIRAGMGPLTIATDLL
                     KPGGYQRLSHMVKDVLSSLPFAPEKPDLTALDTLAEGVFSKPYYRKDWKEGTASIEGP
                     LPLFDCFAAPCIEACPVGQKVPAYVAATGAGNANRGLSIVTSDNPFPTITGILCDHIC
                     QEHCSRVDYEGAVRIRDMKLSTVRAAVPESENEVRPAWPRKAIGNTAIIGAGPAGLTC
                     AWYLAQHGQKAVIFEKLPIAGGVPSTVIPSFRISREDIASDIDKLEKLSVEFRFGTEI
                     STIEACEALKRDEFDSIVIAHGAYHARNLVLDGDGVKVMHALDFLRSYMEKGLEHFSG
                     VKTVLVVGGGNTACDSVRLASRIPGIEHVYWSYRRSRKEMPADKEELANAIAEATALT
                     VGCDDWASFSSQDLSFLRNSSFEENNQESILLELTLPEALEPGMARLRRMKPGEKDES
                     GRPSPVPTDETFLLPCDLIITAVGEAPDPTLFSNLGVEVSKNGLPIVDEETMESSIPG
                     "
BASE COUNT          693 a          612 c          640 g          674 t
ORIGIN      
        1 atgattcttg ccaaacctgg ttatggcaca tttcttactt gggggtatcg atgagcaaga
       61 taatgaaaac tacagcgttg cccgccctat ttgagcgcat tgcgggcgaa tatacaacaa
      121 aacgcagcat attcgaaatt cctgaatcga cctggctgga tctgttcgag caggaagcag
      181 agagtgcagg catgccaata atggcctcca ctataagcat tccgctagga ccagcagctg
      241 ggcctcatac acagattgct cccaacctca tcgctgcata tctttcggga gcgagagtgt
      301 tcgaactaaa aacggtgcag gagaacgatc atctcgacat cgacaaaccc tgtatcgatg
      361 cgctcgacga gggatacaat gtcgaatggt ccactgagct ctctctggaa gaagcacgta
      421 ttgaatatat aaatgcctgg ctggttatca atctttttgc aagaatatgg tcgcataaac
      481 caagcgattt tttattcaat atgtctgtgg gttatactct ggaaggcctc aagagcgcaa
      541 aaatggaggc ttttatcgag ggtatgcgca ggcctgaaac gggctcttat tgggaacgcg
      601 cgctagaaga gctcaaatct ttcgtcgaat ctccactctt tatgcaggct tttggatctc
      661 aggcgctcga gaaagcccgt tctatagcag agcatatgcc agtacggcct gtacattctg
      721 tgacactctc gaccatgcat ggctgccctc ctgaggaaat tgagcgcata gggcgctatc
      781 tcattgagga aaagggcttc gacacctata taaagctgaa cccaaccctt ctaggctatg
      841 aagatacacg aacaattctt gataggcttg gatggaagga tattgtccta aagagcgaga
      901 gttttgagca tgatctgcaa ttcgatatgg cgatatcact catacatacg cttagcgaag
      961 ttgcgttcaa taaaggaagg agatttggca tcaagctctc caatactctt gccaatatca
     1021 ataatggaga ttatctgcct ggttttgagc gatatctctc aggccgggct cttttcccga
     1081 tcaccatcaa ccttgcggca aaactcgctc atgctttgcc tgattttgga gcgcgatttt
     1141 cttattgcgg tggtgtctca gccttcaatg cggctgatct aatcagagca ggaatgggcc
     1201 cactgacaat tgcaaccgat ttgttgaagc cgggcggtta tcagcgcttg agccacatgg
     1261 tgaaagacgt tctttcaagc ctaccttttg cgcctgaaaa accggatttg acagctctag
     1321 atacactcgc cgaaggagtc ttttcaaaac cttactaccg aaaggattgg aaagaaggca
     1381 cagcttccat tgaaggtcct ctgccgcttt ttgattgttt tgcggcgcct tgcatcgaag
     1441 catgtcctgt tggacaaaag gtacctgctt atgtcgccgc tacaggagcc ggcaatgcaa
     1501 acagaggtct ttctattgtg acatcggata atccattccc cacaattact ggaattctgt
     1561 gcgaccacat ctgtcaggaa cattgctccc gcgttgatta tgagggcgcg gtgcgaatcc
     1621 gcgatatgaa attgtcaacg gtccgagcgg cagtcccaga atccgaaaat gaagtgcgac
     1681 ctgcgtggcc aagaaaagct attggaaata cggcgataat cggggcgggg cctgcgggtc
     1741 tcacttgtgc ctggtatcta gcccaacatg ggcagaaagc cgtaatcttt gaaaagctgc
     1801 ctattgccgg cggtgtacct tccacagtca tcccttcttt cagaatttct cgagaagata
     1861 ttgcatccga tatcgataaa ctggaaaaac ttagtgtaga gtttcgattc ggcactgaga
     1921 tttctacaat agaggcttgc gaagcgctca aaagagatga attcgattcc atcgtcattg
     1981 ctcatggagc ataccatgct cgcaatttgg tgctggatgg agatggcgta aaagtcatgc
     2041 atgcactcga ttttctccgc tcctatatgg aaaaggggct tgaacatttt agcggagtga
     2101 aaactgtgct cgttgtgggc ggaggcaaca ctgcatgcga ctcagttcgg ctggcatcaa
     2161 gaatccctgg tatagaacat gtctactggt cttatcgacg cagcagaaag gagatgcctg
     2221 cagataaaga agagcttgca aatgcaatcg ccgaagcgac tgcactcacg gttggttgcg
     2281 atgattgggc ttcattttct tctcaggatc tctcatttct ccgaaattca tcttttgaag
     2341 aaaacaatca agaatccatt cttctcgaat tgacgctccc tgaagcactg gaaccaggta
     2401 tggcaaggtt gcgccgcatg aaaccaggag agaaagatga gtctggacgc cctagtccgg
     2461 ttccaactga tgaaacattt ttgcttcctt gcgacctcat tattaccgcg gtgggagaag
     2521 ctcctgatcc cacactcttt tcgaatcttg gagtcgaagt atcgaaaaat ggtcttccaa
     2581 tagtagacga agagacaatg gaatcttcga ttcccggaa
//
LOCUS       DAOMZL010000154         2585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_40419_length_2585_cov_9.809486, whole genome shotgun sequence.
ACCESSION   DAOMZL010000154 DAOMZL010000000
VERSION     DAOMZL010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40419_length_2585_cov_9.809486"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..597)
                     /locus_tag="PLQ30_08315"
     CDS             complement(343..597)
                     /locus_tag="PLQ30_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02489.1"
                     /translation="MRSKRPRSHVGSTTRAPLWPYESVQFGPHCTVICAVLVVALLGI
                     PATMLFSQDQHPNKLPANTLPLDFSTLEGATAVDHNKHRS"
     gene            complement(587..946)
                     /locus_tag="PLQ30_08320"
     CDS             complement(587..946)
                     /locus_tag="PLQ30_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPW02490.1"
                     /translation="MQKNEKNPYNYQSFDEMLSSRIRLAALALLAGCDEAEFTYIRDV
                     IQTTDGNLASHLRKLEGSGYVRYQKRFIDRKPATYYRITPQGRSALLAYSRRLTELLK
                     NSNYQGGSSNETETDEK"
     gene            complement(918..1520)
                     /locus_tag="PLQ30_08325"
     CDS             complement(918..1520)
                     /locus_tag="PLQ30_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02491.1"
                     /translation="MKEEPFISPDQQLELIERMLEESRKTVLSMGFPSMIWGGFVSIG
                     TALSYAFASWHWYVAIGLLWALLMVSAFLLVVRHDRRKQQQQLKTTSLHKISNEMWIF
                     IFGVMVLYGIVGFLRPQNISLPYALCVVSLLVGTAYWISSSMTRYFLLRIMSALWIVC
                     SIVVLLVPALWSPAVVGICAFLGECMPGIYFYAKERKESV"
     gene            complement(1703..2191)
                     /locus_tag="PLQ30_08330"
     CDS             complement(1703..2191)
                     /locus_tag="PLQ30_08330"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HPW02492.1"
                     /translation="MIRLLKIALVLLGVPVFILSVLGLFWLANNPANPSYAYMLYPIL
                     VGIYLSTIPFYIALYQAFKILINIDKDNALSKQCARALKNIKLCAIAVSILYTGILPL
                     VFFIAQKDDAPGLIVFATIPIFISLVIAIFAAVFQALFGNIMDMSLDNDLIVLDGQTI
                     KR"
BASE COUNT          746 a          543 c          554 g          742 t
ORIGIN      
        1 ctggcaagca agagtatctc gaaaatctaa taatgcagta tgtgctggga gtggactgat
       61 tagatatcac gaatcgagag gtttgtgttt tcaaaagccc ccttttctgt gttctcaatt
      121 ccgcttgctc cagggaggac gcagatagct tcgctgtctt tcctggcctc tttcctggcc
      181 ttacgagaaa cttcacttgc cctacgatga cccagccgcc ctcaagggga cggatgagga
      241 gatcatggcg agcaccagac gaatccgcga tgagatcgag caagccgtcc tggacctcgt
      301 tgcccgtcta tccatctgat tttaagctcc tataggtgca ctctaagacc tgtgcttgtt
      361 gtggtcaact gcagtagcgc cttctagagt ggaaaaatca agaggcaatg tattggcagg
      421 caatttgtta ggatgctggt cttgagagaa aagcatggtc gcggggatcc ccaagagcgc
      481 taccacgagc accgcacaga ttactgtgca atgtggtccg aattgaacac tttcataagg
      541 ccacaaagga gcccgagttg tcgatccgac atggctccta ggccttttac ttctcatctg
      601 tttcagtctc atttgatgaa cctccttggt aattggaatt cttcaataat tcggtcaatc
      661 gacgactata tgccaaaaga gcggatctcc cctgtggggt tattcgatag tatgtagcgg
      721 gtttgcggtc gataaatcgc ttttgatacc gcacatatcc gcttccttct agctttcgca
      781 aatggcttgc cagattgcca tcggtggttt ggatgacatc acgaatataa gtaaattcag
      841 cttcatcgca gccagcaagc aatgcaagtg ctgcaaggcg tatcctagac gaaagcattt
      901 catcaaagct ctgatagtta tacggattct tttcgttctt ttgcatagaa ataaattccc
      961 ggcatacatt cgcctaagaa ggcgcatatt cccaccactg caggcgacca aagggctggt
     1021 accagtaaaa caacaatact gcacacaatc caaagggcgc tcattattcg caagagaaaa
     1081 tagcgggtca tgctactgct tatccaatag gcggtgccaa cgagaagcga tacaacacaa
     1141 agggcgtagg gaagagagat attctggggg cgcaaaaacc cgactattcc atagagtacc
     1201 atcacaccaa agataaagat ccacatctca ttagagattt tgtggagtga tgtggttttt
     1261 agctgctgct gttgcttccg tctgtcatgc cgaaccacta atagaaaagc gcttaccata
     1321 agtaaagccc ataatagccc tatggctaca taccagtgcc atgatgcaaa ggcatagctc
     1381 aatgcggtac ctatgctgac aaaacctccc catatcattg aggggaatcc cattgaaaga
     1441 acggttttac gagattcttc cagcatgcgc tcgatgagct ccaactgttg atctggactt
     1501 atgaaaggtt cttctttcat gatctgcgct cctttggagt tgatactccc aaattgctca
     1561 ttatataagc agcatatcac aaagtacttt gaattgcaaa gtagtgatta caattttatc
     1621 tcattcgatt tctatatttc ttatttgctc tattttccgg ctttctgttt cattatcgta
     1681 cacatagtca ttgggcgcca aattaccgct taatagtctg tccgtctaag acaatcagat
     1741 cattatctag gctcatatcc atgatatttc caaataatgc ttgaaagaca gcagcaaaga
     1801 tcgcgatcac caatgaaata aagatgggaa tggttgcaaa tactatgagg cctggggcat
     1861 cgtctttttg tgctatgaaa aagactaatg gcaatattcc cgtatataaa atactgactg
     1921 cgattgcaca gagttttatg ttctttagag ctcttgcaca ttgttttgat agagcattgt
     1981 ctttatcaat attgattagg atcttaaaag cttgatacag tgcaatataa aacggtatcg
     2041 ttgataaata tatgccaacc aaaatggggt atagcatata agcatagctt ggattcgctg
     2101 gattattggc tagccaaaat aaccctaaaa cactcagaat aaaaactggg actccaagga
     2161 gaacaagtgc tattttcaaa aggcggatca ttctttgtat ccttataaga ctctcattta
     2221 attattggcc atttgggttt tatcatatat tttattgttt atcaatcaat atcaattgaa
     2281 attttaggtt tgcaatttga actcgagctt tcattactgc tacgaacctt gtgtatgaca
     2341 atgaagcaga atggcggaga atagagtaaa taaggaataa agaaatcgaa ggggaataat
     2401 atagtaattc cttcgctgta tttaaccatg agtcataagt attcttcttg ctgtctgctt
     2461 gctttataca caagattcgt atcagtatgg attgttaatg gaggctgaga gaatggcggt
     2521 aagaaaaggt tatattagac cttcacctat cgatgcggac atcggcgcac aattggagtt
     2581 attaa
//
LOCUS       DAOMZL010000155         2525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_41918_length_2525_cov_5.451012, whole genome shotgun sequence.
ACCESSION   DAOMZL010000155 DAOMZL010000000
VERSION     DAOMZL010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41918_length_2525_cov_5.451012"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLQ30_08335"
     CDS             <1..224
                     /locus_tag="PLQ30_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02493.1"
                     /translation="SIDPFVTILSFQIHKRIHSAYWDAHIRNNTTNYLYRKYNSILKE
                     SLDYIDGISLVPKLENDSSKVAIAYTIAF"
     gene            complement(258..749)
                     /gene="msrA"
                     /locus_tag="PLQ30_08340"
                     /pseudo
     CDS             complement(258..749)
                     /gene="msrA"
                     /locus_tag="PLQ30_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882079.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
     gene            complement(1099..2013)
                     /locus_tag="PLQ30_08345"
     CDS             complement(1099..2013)
                     /locus_tag="PLQ30_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HPW02494.1"
                     /translation="MSTRTFTFTDPDGLEIFVYEWLPDDQKPKAVVQVAHGMAEHALR
                     YERFAKYLNEAGYAVYADDHRGHGKTAKDLSKAGIGGVDSWNGMVNDENELKEIIKKE
                     NPGLPLFFFGHSMGSFIAQRFIELWGSDLKGAILSGTTGLAFIPKEAESALEQAAAGE
                     LRDKYPEGPGLFESINAAFAPTKTPFDWLSRDEAEVQKYIDDPWCGFAFSNGMMLDMA
                     RGLWELYDPRNQALIPKNLPILIIAGEKDPVGANDGVKALVQRYKELGIKDVKMILYP
                     GARHEVLNEINRDEVHQDVKDWLEEHLK"
BASE COUNT          644 a          610 c          528 g          743 t
ORIGIN      
        1 ctagtataga tccatttgtg acaatcctat ctttccaaat tcacaaaagg atacatagcg
       61 cttattggga cgcgcatatt cgaaataata caactaatta tctataccgc aaatacaatt
      121 cgattttgaa agaatcctta gactatatcg acggaatcag cttagtacca aaactcgaaa
      181 acgactcttc gaaagttgcg attgcatata caattgcttt ttgataaaag agaatacctg
      241 aacatcttct agcagcttca ttccagaggc ttagtcgcaa ggctcaagtc cacatggcag
      301 tagccgctag gattcttccg taggtaattc tggtgatatt cttcggctgg gtagaagggc
      361 atggcgggca caagttcggt gacgatgggc tttgagtagc gtccttccac ggtgagctcg
      421 gcgatgattt tctcagatat agcgcgctgc tcgtcgctct gatagaagat tatggaacga
      481 tactgcgtcc caaaatctgc gccttggcgg ttacgctggg tggggtcgtg catgcggaag
      541 aagtggcgca caagttggtc gtagcttatc ttggctgggt cgaactcgat gcggatcgat
      601 tctgcgtggc cggttgtgcc agtgcaaact tgctcgtacg tgggattcgg ggttgtgccg
      661 ccgcagtagc ccacgatagt cgagagcaca ccaggtaggc gccggaagta ttcttcacta
      721 ccccagaaac agcctgcagc aaagatagcg gcctgcctcg tgtcagtctc agcttgaata
      781 ggcgtatcct gtggactaaa taaaggcatc aatgcgctac cccctttttc atatttttgc
      841 ctataaatgg ctcactcatt tatcttttaa gacattcggt tcatccctaa ttatcttgct
      901 atttgtccaa aatcccgcct atccttaatt atcttgtcat ccagtctaaa atcccgtcta
      961 tcccattgaa atcgaaaaat gtattttcat tgtattcaaa taatccaaaa taatgctttg
     1021 catttagagc aatctatgca ttctcatctc atgaggatct gcgatcacct caactggcat
     1081 ggttggaaga ccggttttct atttcaaatg ctcttcgagc caatccttca catcctgatg
     1141 gacctcgtca cgattgatct cattgagcac ctcatgcctt gctccgggat ataggatcat
     1201 ctttacatcc ttgatcccca gttctttgta tctctgaacc agcgccttta cgccatcatt
     1261 cgctccaaca ggatcctttt ctccagcaat aataagaatg ggaagattct ttgggataag
     1321 tgcttgattc ctcgggtcat aaagctccca caggccgcga gccatatcca gcatcatacc
     1381 attggagaaa gcaaaaccac accaagggtc atcaatgtac ttttgaactt ccgcctcgtc
     1441 gcggctgagc caatcaaaag gagttttggt tggtgcaaag gccgcattga tgctttcgaa
     1501 tagacctggg ccttcagggt atttgtcccg tagctcgcct gcggcagctt gctccaaggc
     1561 tgactctgcc tcttttggaa tgaaagccag gccagtcgtt ccagaaagta tcgcaccttt
     1621 tagatcgctg ccccacaatt cgatgaacct ctgtgcgatg aatgagccca tgctgtgacc
     1681 aaagaagaaa agcggcaaac ctggattctc ttttttgatg atctctttca gctcgttttc
     1741 atcattgacc atgccgttcc agctgtcgac tccgccaatt ccagctttag agaggtcctt
     1801 ggctgttttt ccatgaccac gatgatcatc tgcatagacc gcatatccag cttcattgag
     1861 atattttgca aatcgctcgt atcgcagcgc gtgttccgcc ataccatgcg ctacttgcac
     1921 tacggctttt ggcttctggt catccggtag ccattcatag acaaaaatct ccaaaccatc
     1981 gggatccgtg aatgtgaaag ttcttgtgct catacgcctc ctccttatat ttgtatgcat
     2041 ctgtatgcat ttgcgcttta atcattgact acgcaaagaa tacaaaactt ggattataac
     2101 gaattttcta tttctgggtt cgctccaaag gcggactctt ttattataca tcagcctgca
     2161 gctctatcaa gatatgtcca atatgtgcag atttttccct tagatattat ttacataaga
     2221 attgccaaga aaggctctta tctcgatact tgacaggtta agaaaaatgt catgcaattc
     2281 tttatcttga ctttgccagt ttgttttcta ataaagtgtt ataatcactt atcgtataat
     2341 gactagcgca catctagtca gatcataaat ctgtagtacg tattctactt gctgtgcttt
     2401 gtctgggcaa gtagatggcg aatctcttgt acggtgagcg actggcgaga aaaatcaatc
     2461 ccaaggtctt ctccaatcgc ctcaaggact gcgtcatagt agtcaaacac atagagcttc
     2521 tcatc
//
LOCUS       DAOMZL010000156         2501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_42534_length_2501_cov_15.495912, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000156 DAOMZL010000000
VERSION     DAOMZL010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42534_length_2501_cov_15.495912"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1284)
                     /locus_tag="PLQ30_08350"
     CDS             complement(133..1284)
                     /locus_tag="PLQ30_08350"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW02495.1"
                     /translation="MSLLKKKILILMMEVGMGHKIPALAIKEELERKYPDRFDISVLD
                     FARECGALGVDRQLKKGWDIALSFPFSVRIGYRFIELIKNNFFYIELLCRDFIKKGMD
                     YIANTKPDAVLATHTLCLYIACKARRTFGGNYKVLCCVVDPFDGYSLWANLDTDLFFV
                     ATEQSKKRLIDHGVNASKILVTGFPMRKSFFVFMKNKDNIVHELGLDPAKCTILISAG
                     SRGISNVYSFAECVKKKGIPFNIISVAGKNQQLKEHLDTLASIKNETNLVSLGYVDTM
                     NELMAASDVIAGKAGASTFMEALFLKKPMIFTGWTAYNDWYIIDFALKHNIGWYCPTL
                     RSFLTIISKLSSEPHMVHEYSERIEALHFTSGTETIADIVACEFGNERL"
     gene            complement(1370..1519)
                     /locus_tag="PLQ30_08355"
     CDS             complement(1370..1519)
                     /locus_tag="PLQ30_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02496.1"
                     /translation="MRNGYLHFSWHPVLIVPGDDLDFVAESKNLKAIIKIISKRRRSV
                     LSIPF"
     gene            complement(2045..2323)
                     /locus_tag="PLQ30_08360"
     CDS             complement(2045..2323)
                     /locus_tag="PLQ30_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02497.1"
                     /translation="MYSGHKESRECLLTLVERKTRLEVILKLPNKAAVAVRQAFDQLE
                     RQLGGELFRTMFRSITLDNGVEFSLVYDLERAVLTKDTRTTLYFAHTL"
     gene            2309..>2501
                     /locus_tag="PLQ30_08365"
     CDS             2309..>2501
                     /locus_tag="PLQ30_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02498.1"
                     /translation="MSTVHRVSFSMPCFLTRNNILGTFFYAPYGKRNGGVLGHPGLKD
                     PYLPGQHCFWPILRSYHIEN"
BASE COUNT          687 a          557 c          565 g          692 t
ORIGIN      
        1 gacattgcca cgaggtatcg aactatttta gagaaatagc ttcgaaaatg aggctcgtgc
       61 catcgagaaa gagattagaa ttgaaataga aatgagcaga catcgatatg ataaggagcg
      121 atgaagccct atctatagtc tctcgtttcc aaattcgcaa gcaacgatat ctgcgatggt
      181 ctctgtgcca gaggtaaaat ggagcgcctc gattcgctcg ctatactcgt gcaccatatg
      241 aggctcgctc gagagcttgc tgataatggt gagaaaagat cgaagagtgg ggcagtacca
      301 tccaatattg tgtttcaatg caaagtcgat gatgtaccag tcattgtagg cggtccatcc
      361 cgtgaaaatc atcggctttt tcagaaagag cgcttccatg aaggtcgatg cgcctgcctt
      421 tcctgcgatg acatcggaag ccgccataag ttcgttcatg gtatcaacgt atccaagaga
      481 aaccaggttt gtctcatttt tgatggatgc aagagtatca aggtgttcct tgagttgctg
      541 attttttcct gcgacagaaa tgatgttgaa aggaataccc ttctttttta cacactctgc
      601 aaacgaatag acatttgaaa tacctcgaga accagcagaa atgaggattg tacactttgc
      661 agggtcgaga ccaagttcat gaacaatatt gtctttgttt ttcatgaata caaagaagct
      721 cttgcgcatt gggaatcctg tgacgagtat cttcgatgca tttacgccat gatcgataag
      781 gcgttttttc gattgttcag tcgcgacgaa aaagagatcg gtgtcaaggt ttgcccagag
      841 actgtaacca tcaaagggat cgaccacaca gcacaaaacc ttgtaattcc cgccaaaggt
      901 gcgcctcgct ttacacgcga tatacagaca aagtgtgtga gttgcaagga ctgcatcagg
      961 cttagtgttt gcaatataat ccattccctt cttgatgaaa tctctacaga gaagctcgat
     1021 ataaaagaaa ttatttttga tcaactcaat aaagcgatat cctattcgaa cgctaaaagg
     1081 aaaagagaga gcgatatccc atcctttctt aagttggcgg tctactccta gggcaccgca
     1141 ctctctcgca aagtcgagaa cgctgatatc aaatctatcc ggatattttc tttccaattc
     1201 ttcctttatt gcgagggcag gaattttatg gcccattcca acttccatca tcaatatcag
     1261 aattttcttt ttcaagagag acatgagagc ccccattttt tctggatcaa taggcaccta
     1321 ttgcgataat gatgccgcca ttgacagcta gaagataatt catcacatgt caaaagggaa
     1381 tagataggac tgatcgtctt ctctttgaga ttatcttgat gattgctttg agattcttcg
     1441 attcagcaac aaaatcgaga tcatcgccag gcacgatcaa cacagggtgc caagaaaaat
     1501 gaagataccc attcctcata gagggcatcg agcccttcga gatagctatc agagatcgac
     1561 ggctcaaact ctctttcctt ctgagatgga tcgatcttgg actactgagt gaggatcctc
     1621 gctgagcccg cgatgcatct gggctcgata cggcaaaaaa caatataaaa tctaacttta
     1681 ctatggaggt ccatgcgggc gaatatagaa tttatctatt tccttctctg cccacgccta
     1741 ggtaggctac actgactttt gggttggaag agagttctga ggtattgcct tgcaaatcaa
     1801 ctttgcccaa ttcgagaatg tatcctctgg aagccagctt cagggctttt cgagcgtttt
     1861 gctcaacgct aaggatggct acttttcctc ttggtgaatt ttctggatca tatcgatcat
     1921 atcgaagatc ttattgatga ggattggcgc aaggccaaga gaaggttcgt cgaggaggag
     1981 taagccagct gctgacacaa gtctcctgta aagagcaatg tcgactaaat tgattcttag
     2041 gtttttataa ggtatgagcg aaatacagag tcgtccgtgt gtctttggtc aacaccgcgc
     2101 gttcaaggtc gtagacaagg gagaattcga caccattgtc cagggtaatg gagcgaaaca
     2161 tcgtccgaaa aagctcgccg cccaactgcc gttcaagctg gtcaaaggcc tgtctaaccg
     2221 caaccgcggc cttatttgga agtttaagta tcacttcaag ccgggtcttt cgttccacca
     2281 gggtaagtag gcactcccgt gattccttgt gtccactgta catcgtgtct ccttctcaat
     2341 gccctgcttc ttgacgcgta acaatatcct tgggacgttt ttctatgctc cttacggcaa
     2401 acgcaatggt ggcgtgctcg gacatcctgg cctaaaggat ccttacctcc ccggtcagca
     2461 ttgtttttgg ccgattttgc gttcatatca tatagagaat c
//
LOCUS       DAOMZL010000157         2446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_44000_length_2446_cov_15.254287, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000157 DAOMZL010000000
VERSION     DAOMZL010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44000_length_2446_cov_15.254287"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="PLQ30_08370"
     CDS             <1..767
                     /locus_tag="PLQ30_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPW02499.1"
                     /translation="TVVGRRINQLASEHFAKVQLEMGGKNPAIVADYKDLNRAAAEIA
                     QAAYAVSGQRCTAVSRVIVLKEHAEELETKIIEKAKSHILGNGMRPDVTMGPIITKEA
                     GEKIMEYIHGAEKEGAMIKYGGRHLTGGEFDKGFYIEPTLITDVTPSMKVAREEIFGP
                     VLVSIKVDTFEEAIEVANNTEYGLASCLFSDNLKYIYEFQQEIESGMTHVNHGTITDA
                     SMPFGGAKNSGLGPFSKGATNKDFYTNYKVNYVKYI"
     gene            863..1315
                     /gene="aroQ"
                     /locus_tag="PLQ30_08375"
     CDS             863..1315
                     /gene="aroQ"
                     /locus_tag="PLQ30_08375"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPW02500.1"
                     /translation="MESNHIAIIHGPNLNTLGQREIGIYGGKTLEEINDEIRLEASKF
                     PVSLDFFQSNVEGFIIDFIHGCRGKIDGIVINAGAHTHYSIALRDAIAAVKLPTIEVH
                     ISNIYKREAFRHVSIISPVCIGQICGFSSYSYILGLHALLEVLAEKDK"
     gene            1351..1788
                     /gene="aroQ"
                     /locus_tag="PLQ30_08380"
     CDS             1351..1788
                     /gene="aroQ"
                     /locus_tag="PLQ30_08380"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPW02501.1"
                     /translation="MKKFLSISGPNLNLLGQRKTSIYKEASLDEIHSKLKKKAAELGV
                     EVDCVQFNSEGDIIDCMQTVPGKYEGVILNAGALAHYSYALRSAIGTMLVPCVEVFIS
                     NVYAREDFRHKSVLADVCVGVISGFGANSYILALEALVNLVKP"
     gene            1857..>2446
                     /locus_tag="PLQ30_08385"
     CDS             1857..>2446
                     /locus_tag="PLQ30_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPW02502.1"
                     /translation="MNMRWAKRVDRFLDIGQAKEQGFQIVQLPVAEIMRLDDRAFRDV
                     KIDLLESGLSFEVFESPLPEGVNTTERGFNIYAWIEYLKKAIGRISELGCKTLVWGDG
                     QARILPEEGEATVLKENFNQFLFMVAEIAEKKDIVFCLEPLGPKKTNFLNSLEEVASV
                     LGSVGKKNLAITIASSDIVETNMKIEDIVHYGDLIA"
BASE COUNT          754 a          443 c          605 g          644 t
ORIGIN      
        1 caacagtcgt tggacgaaga atcaatcagc ttgcctctga gcattttgca aaggttcagc
       61 ttgaaatggg aggaaagaat ccagcgattg tggctgatta taaagatctc aatagagcag
      121 cggcggaaat cgcgcaggca gcctatgcag taagcgggca gcgctgcaca gcggtaagcc
      181 gtgttatcgt attgaaagaa catgcagaag aactagagac aaagataatt gaaaaagcaa
      241 aaagccatat tctcgggaat ggaatgcggc cagatgttac catgggtccc attattacaa
      301 aggaagctgg cgaaaagata atggagtata ttcatggagc agagaaagaa ggagcgatga
      361 tcaaatatgg aggtcgccat cttactggcg gagaattcga taagggattc tatatagagc
      421 ccacgctcat tactgatgtc acgccctcaa tgaaggtagc tcgagaagaa atctttggtc
      481 ctgtgcttgt cagtattaaa gtcgatacct tcgaggaggc gatcgaagtc gccaataata
      541 cagaatatgg tcttgcctct tgtctatttt ccgataacct aaaatacatc tatgagtttc
      601 agcaggaaat agaatccgga atgactcacg ttaatcatgg aacaattacc gacgcctcta
      661 tgccatttgg aggcgcaaag aattctgggc tcggtccatt ttctaaaggt gctacgaata
      721 aggatttcta tacaaattac aaagtcaact atgtgaaata tatctaaaca agatacctca
      781 taagcagcat aagcaggcgt cgcgctacat attcctcgtt taagaataat agagtacaat
      841 ggctcttaaa tcaggaatgc ttatggaatc gaatcatatt gcgataattc atggtcctaa
      901 cctgaatacg cttggacaga gagaaatcgg aatttatggt ggcaagactc tggaagagat
      961 caatgatgaa attcgattag aggcatcgaa atttccggtc agcctggatt tctttcaatc
     1021 gaatgtcgag ggattcatta tcgattttat tcatggctgt agagggaaaa tagatggaat
     1081 cgtaatcaat gccggtgccc atactcatta ctcgattgct ttgcgcgatg caatagccgc
     1141 tgtcaaactt ccaacaattg aagtacatat atcgaatatc tacaaaagag aggcttttcg
     1201 gcacgtatct atcatctcgc cggtatgcat cgggcagata tgcggcttta gctcttatag
     1261 ttacatactt ggtctccatg cattgctcga ggtccttgcc gaaaaggaca aataatattc
     1321 atcgataagt tcagagagga gaaaagagaa atgaagaagt tcttatcgat ttctgggcca
     1381 aatctaaatc tgctggggca aagaaagact tctatttata aagaggcttc actggacgaa
     1441 attcattcta agctaaagaa aaaggctgca gaactaggtg tagaagtgga ttgcgtgcag
     1501 ttcaatagcg aaggcgatat catagattgc atgcagacag tgcctggcaa atatgagggg
     1561 gttatcttaa atgcgggagc tcttgcacac tacagttatg ctttgcgcag cgcaattgga
     1621 acgatgttag tgccctgtgt agaggttttt atatcgaatg tgtatgcgag agaggatttt
     1681 cggcataaat cggtccttgc cgacgtatgt gttggagtta tctcgggatt cggtgcaaac
     1741 agctatatcc tggcgcttga agcacttgtt aatctggtta agccttgaag aaagctctga
     1801 tataagtttt agcggaagcc tcaaattgag gagcggctat gaatggggaa gccactatga
     1861 atatgagatg ggcaaaaaga gttgatcggt ttttggatat aggacaagcc aaagagcagg
     1921 gatttcagat tgtgcaactg cctgtggccg aaataatgcg gctcgatgat cgtgcatttc
     1981 gagacgtaaa gattgatctt cttgaaagcg gattatcatt tgaggtattt gaatccccgc
     2041 ttccggaagg tgtcaatact accgagagag gctttaatat atatgcgtgg atcgaatatc
     2101 tgaagaaggc aattggaaga atttctgaac taggatgcaa gacattggtt tggggagatg
     2161 gccaggcgag gattcttcca gaagaaggcg aagccactgt cctaaaagaa aatttcaatc
     2221 agtttttatt catggtcgca gagattgccg aaaaaaagga catcgttttt tgcttggaac
     2281 cgcttgggcc taagaagacc aatttcttga attcgttgga agaagttgcc agcgttctgg
     2341 gctcggttgg gaaaaagaat ttagccatca ccatagcttc cagcgatatt gtcgagacaa
     2401 atatgaaaat cgaagatatc gttcattatg gagacttgat tgccca
//
LOCUS       DAOMZL010000158         2439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_44199_length_2439_cov_20.031040, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000158 DAOMZL010000000
VERSION     DAOMZL010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44199_length_2439_cov_20.031040"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PLQ30_08390"
     CDS             complement(<1..614)
                     /locus_tag="PLQ30_08390"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPW02503.1"
                     /translation="MEQQAVRMPLLGDSFPQVSLQTTQGMLNLPGDLKGSWFILFSHP
                     ADFTPVCTSEFVAFQKLIPEFEKLGVKLIGMSIDQITSHIKWLEWIKEKLGVEITFPL
                     AAANDSIAMTLGMLHPGKGTNTVRAVYIVDPNGIIRLILYYPQEIGRFIPEVLRAVKA
                     LQISDKNHVSMPANWPDNELIGDHVIVPPPKTVAEAADRLQHYEG"
     gene            800..2416
                     /locus_tag="PLQ30_08395"
     CDS             800..2416
                     /locus_tag="PLQ30_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPW02504.1"
                     /translation="MDRIKGESCLDSTEISVLNDCMSLDTDNSVLYPNNAPLGLLSLG
                     ARTQLLQPDRVLEIVFQSLKELVDYELAVVLSIEQGNVLRVRKAFGPLAIPALENFSI
                     GLDQRKDIAEILQSKQPKLFHQSEAHIDTYYGIIDLPSGHSCLVSPLYIEDLLVGMLT
                     IDHRSCNRFSPSIVQFIGTISRLISVSLIQTDASTDLLKRTRVLLDERNRLLDENAQV
                     FHDLIGVSPAWMRVLDDIRLVAASELPIFITGETGTGKEQVARTIHAMSTRNQGPFIA
                     VNCSGLVTTLAESELFGHEKGAFTGATTSRRGRFEIADGGTLFLDELGDLPLEIQPKL
                     LRVLQDGIIERVGAEHPLHVDVRIIAATNKNLEDEMAKGKFREDLWYRINVFPIHLPP
                     LRERDHDAILIAQYFLEKIRKRPGYNSVEFSDQAIDKILRIPWYGNVRELRNSIERAA
                     ILAKGGIIHAEHIISYRDQRQAMHENVLIEKSEIGSLEEIEKHHIEKILMQTNGKIYG
                     TDGAAAILGLKPSTLQSKMKKLGIRRPGRS"
BASE COUNT          705 a          554 c          532 g          648 t
ORIGIN      
        1 ccttcataat gctgaagacg atccgcagcc tcggcaactg tctttggtgg cggaacaatc
       61 acgtggtcgc caattagctc attatctggc caatttgccg gcatactgac atgattctta
      121 tccgaaatct gtaatgcctt taccgctcga agaacctcag gtataaagcg tcctatttct
      181 tgtggataat agagtataag acgaataatg ccattcgggt ctacaatgta gactgctcgc
      241 acggtattgg tgcctttccc ggggtgaagc atgcctagag tcattgcgat agaatcgttt
      301 gctgcggcga gagggaaggt gatctcgaca ccgagttttt cctttatcca ctccagccat
      361 ttgatatggc tcgttatctg atctatcgac attccaatca gcttgacgcc gagcttttcg
      421 aattctggaa ttagtttttg aaaagccaca aattcacttg tgcacacagg cgtaaagtca
      481 gcaggatgac tgaaaagaat gaaccacgaa cctttgagat cgccaggtag atttagcatt
      541 ccttgcgtag tctgaagact gacttgagga aaagaatccc ctaaaagcgg catacgtact
      601 gcttgctgtt ccatagcaaa ctccttttaa gacaagattt ctgcattgtg atgcacccta
      661 ctctctttgt aagaataggc acaagaatct aaagcaagaa tagtgccaat taactgcaaa
      721 agaataataa agctgcttga atatctaagt ccttatcggt caatgctttt tcagattgct
      781 ctcattgact gatgggtgga tggacaggat aaaaggagag agttgtcttg acagcaccga
      841 aatatcggta ttaaatgact gtatgtcgtt ggatactgat aattcggtac tctatcctaa
      901 taatgctcct ttgggattgt tgtctttagg cgcgagaacc caattgctgc aaccggatag
      961 agtactcgag atagtatttc aatccttgaa agaactagtc gactacgagc ttgctgtggt
     1021 tttatccatc gaacaaggaa atgtgcttag agtccgaaaa gctttcggtc cactagcaat
     1081 accagcccta gaaaattttt ccattggtct cgatcagcgc aaagatatcg cagagattct
     1141 ccaatctaag caacctaagc ttttccatca atccgaagcg catatagata cttattacgg
     1201 aattatcgat ttgccatccg ggcattcatg ccttgtttcg ccactctata ttgaagacct
     1261 tcttgtgggc atgcttacca ttgaccatcg ttcctgtaat cgattctcac catctattgt
     1321 gcagtttatt ggaacaatct cgagactcat ttcagtttcg cttattcaaa cagacgcttc
     1381 tacagatctt ttaaaacgca ctcgtgtgct tctcgacgaa cgaaacagac tgctcgatga
     1441 gaatgcacag gtttttcacg acctcatagg cgtatctcca gcgtggatgc gcgtcctgga
     1501 tgatatacga ctcgttgcgg cttcagagct ccccattttc attacaggag agactggaac
     1561 tggcaaagag caagtagcaa gaaccattca tgcgatgtcg acccgaaatc agggaccctt
     1621 catagctgtc aattgttccg gcctcgtgac cacacttgcc gagagcgaac tttttggtca
     1681 tgaaaaggga gcttttacag gcgctactac ctctagacga ggccgattcg aaattgccga
     1741 tggtggtact ctttttctcg atgaattggg agatttgccg ctcgagattc aacctaaact
     1801 ccttcgcgta ttacaggatg gcataataga gcgcgtcgga gcggagcatc ctctccacgt
     1861 cgatgtacgt atcatcgccg caacgaacaa aaacctcgaa gacgaaatgg caaaaggcaa
     1921 gtttcgagaa gatctatggt ataggatcaa tgttttccca attcaccttc caccacttcg
     1981 cgaacgcgat catgatgcaa ttctcattgc tcaatatttc ctagaaaaaa taagaaaaag
     2041 gccaggatat aactctgtgg aattctccga tcaagcgata gacaagatct tgcgaattcc
     2101 ctggtatggc aatgttcgcg agctaagaaa tagcatagag cgcgccgcta ttcttgcaaa
     2161 aggaggcatt atccacgcgg agcatataat ttcctataga gaccaaagac aagcgatgca
     2221 tgagaatgtg ctcatagaaa aaagcgaaat aggatcacta gaggaaatcg agaaacatca
     2281 tatcgagaag atcctcatgc aaaccaatgg aaaaatctat ggtaccgatg gagctgccgc
     2341 aatactcggt ctcaaaccca gcacccttca aagcaaaatg aagaaattag gaatccggcg
     2401 acctggaaga tcatgaacag tgaggatctt catgtctct
//
LOCUS       DAOMZL010000159         2433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_44353_length_2433_cov_17.522288, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000159 DAOMZL010000000
VERSION     DAOMZL010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44353_length_2433_cov_17.522288"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..1190)
                     /gene="dctP"
                     /locus_tag="PLQ30_08400"
     CDS             complement(156..1190)
                     /gene="dctP"
                     /locus_tag="PLQ30_08400"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPW02505.1"
                     /translation="MKKLGLMVLLVCIVLSGAFGQGKVYELKLAHADPTDPAVSRKQA
                     QAIAFASMVNSRSGGRINVKIFGGGALGAEREYIEGIKAGTIEAGLASGPVADFFPPA
                     AITDIPYLFPTSKVAWDVMDGAFGDKLRDMFLKQTGMRCLAFGEVGFRHFTNSVRPIK
                     SPSDLKGMKIRVMETPVYVNMLRAAGANPTPIAWTETYTALQTHVVDGQENPIASIIM
                     AKLYEVQKYITLDGHSYGVDWFIINEKFFQSLPPDLQYIVVDTAQTVAAVGRGFITLQ
                     NAQGLQTLIDAGMQVYSPTDAEMQQFKKVMQPAVIDWLKTKVDPALINELLKAVDDAI
                     VAQKAKEGLK"
     gene            complement(1243..2205)
                     /gene="iolN"
                     /locus_tag="PLQ30_08405"
     CDS             complement(1243..2205)
                     /gene="iolN"
                     /locus_tag="PLQ30_08405"
                     /EC_number="3.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF041098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-scyllo-inosose hydrolase"
                     /protein_id="HPW02506.1"
                     /translation="MSGWKFPQEGQMDKPSKIYFQTMTKYDVEERLKTNDLILIPIGS
                     TENHGNAGPYGEDTFIVTRIAELVAKQVGCTVAMPMWYGSHPFHHIGQPGTVPLSDDV
                     FTAHIRAMISGFWNAGFRKQIFLSMHGQEYALPSAIQEWGKKYQVPAIVIFLDLPRIM
                     GTDLKDKEHGGPFETPFQHADEAETSISMALFPELCQPEHAENTSLKGFLPPGHVDRG
                     GDIYGNPIPGHCIIGGTGIECVTFPEGVLGKAKLADPNKAKKAIENVLDYMVRLHNDI
                     MERFPVGKLPEIKLMTQRDEKEIELVLKGPLNGGKHIYTIAWPS"
     gene            complement(2227..>2433)
                     /locus_tag="PLQ30_08410"
     CDS             complement(2227..>2433)
                     /locus_tag="PLQ30_08410"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPW02507.1"
                     /translation="HETEEEVMFFLEGEGKFITQDQEIDLKPGICVYNPPGAPHKIVN
                     TGTTKLKFVWVYAPQLKSHRIPNN"
BASE COUNT          594 a          604 c          510 g          725 t
ORIGIN      
        1 agactttgtt ccttcatcct ttgcccctta atctcccata agaattacag caaaatccta
       61 gaaaggatgc ggtgctcaaa aacactctta aaatgccaac aaaagggcgc aggaacgcaa
      121 tttagatcgt ccctgcgcat taaatccgca gactgctatt tgagtccttc ttttgccttt
      181 tgagccacta tggcatcatc tactgctttc aatagttcat tgataagtgc cggatcgacc
      241 ttagttttaa gccaatctat aaccgctggc tgcatgacct tcttgaattg ttgcatctcg
      301 gcatcggttg gagagtaaac ctgcatcccc gcatcgataa gagtctggag accttgggca
      361 ttctggagtg tgataaagcc acgacctact gcagcaactg tctgtgcggt gtccacaact
      421 atgtattgca gatctggcgg caagctctga aagaatttct cgttgatgat aaaccaatca
      481 actccatagc tgtggccatc gagcgtgatg tatttctgta cttcatatag ctttgccatg
      541 attatcgaag cgattgggtt ctcttgtcca tcgacaacat gggtctggag agctgtatag
      601 gtctcggtcc atgctattgg cgtcggattt gcgccagcag ccctaagcat gttgacatat
      661 accggagtct ccataacgcg tatcttcatc ccttttagat cgcttggact tttgatagga
      721 cgaacactat ttgtgaaatg cctaaagccg acctctccaa aagccagaca gcgcatacca
      781 gtctgtttaa ggaacatatc tcgaagcttg tcaccgaagg ccccatccat cacatcccag
      841 gccaccttac ttgttggaaa aagataggga atatcggtga ttgcagcagg agggaaaaaa
      901 tctgctacag gcccggaagc aaggccagct tcgatagtac cagccttgat tccttcgatg
      961 tattcacgct cagctccgag agctcctcct ccaaagatct ttacattgat acgtccgccg
     1021 ctcctggagt tgaccatgct tgcaaatgca atagcctgcg cttgctttct tgagaccgca
     1081 ggatcggtag gatctgcatg tgcaagcttt agctcatata ccttgccctg tccaaaagct
     1141 cctgatagca caatgcatac caggagaacc atcaatccca attttttcat aggaatacct
     1201 ccttccttat atgatgaagc cttgcttcgt ttttccattt atctagctag gccaagcaat
     1261 tgtatatatg tgctttcctc catttagcgg acctttgaga acgagttcga tttccttctc
     1321 gtctctttga gtcataagct tgatttctgg cagcttgccg acaggaaaac gttccatgat
     1381 gtcattgtgt agtctgacca tataatccag gacattttcg atcgctttct ttgctttgtt
     1441 cggatcagcg agttttgctt ttcctagaac accttcgggg aacgtcacac attcgatgcc
     1501 tgtcccgcca attatgcaat gcccaggaat cgggttgcca taaatatctc cgcctctatc
     1561 gacatgtcct ggcggaagaa aacctttcaa tgatgtattc tcggcatgtt ccggttggca
     1621 tagttccgga aacaatgcca tcgaaatcga agtctccgct tcatcggcat gctggaatgg
     1681 cgtttcgaaa ggtccaccat gttctttatc tttgagatct gttcccatta ttctcggcag
     1741 atcaaggaag atgacgattg ctggaacctg atatttcttt ccccattctt gaattgccga
     1801 tggaagggca tattcttgac catgcatact gaggaatatc tgcttacgga agccagcgtt
     1861 ccagaatcca gatatcattg cgcgaatatg agccgtaaat acatcgtcag atagaggtac
     1921 ggttcctggt tgcccaatat gatgaaatgg atgagaacca taccacatcg gcatcgctac
     1981 cgtacagcca acttgcttcg ctaccaattc cgctatcctg gtcacgataa aagtgtcttc
     2041 accgtatggc cccgcatttc catgattttc ggtcgaccct ataggaatta gtatcaagtc
     2101 gtttgtcttc aatctctctt ctacatcgta tttcgtcata gtctgaaaat agatttttga
     2161 gggcttgtcc atctggcctt cttgagggaa tttccatcct gacatctctt gttctccttt
     2221 catttcttaa ttattcggaa tcctatgact ctttagctga ggggcataaa cccagacaaa
     2281 cttgagttta gttgtgcctg tattcacaat tttgtgcggg gctccgggtg ggttgtagac
     2341 acatattccc ggttttaggt ctatctcctg atcttgggtt atgaatttgc cttcgccttc
     2401 cagaaaaaac ataacctctt cctcagtctc atg
//
LOCUS       DAOMZL010000160         2399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_45348_length_2399_cov_19.099829, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000160 DAOMZL010000000
VERSION     DAOMZL010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45348_length_2399_cov_19.099829"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /gene="nrdD"
                     /locus_tag="PLQ30_08415"
     CDS             complement(<1..1078)
                     /gene="nrdD"
                     /locus_tag="PLQ30_08415"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002678883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HPW02508.1"
                     /translation="MTDKTEIPSLFGHDFLGWQVAPPIKSVVKRSGEVVSYDRHKIEK
                     AIEKAFIATNTSILDNQGNSKPILYTDLVEKKIRELIQTRHPNSIPAIEEIQDLVETV
                     LIEQGETAVAKAYILYRARHEAIRDTKKLMLDISATMNGYLARSDWRVNENANVNYSL
                     GGLILHNSGSVTANYWLTQIYPKDIAEAHRNADFHIHDLSMFSGYCAGWSLRDLIREG
                     LGGIPDKISSKPASHLSTLANQMVNFLGILQNEWAGAQAFSSFDTYLAPFVRADSLSD
                     KEIKQSLQSFVFGVNTPSRWGSQAPFTNITLDWTVPEDLRDKPAVVGGKELDFSYGDC
                     KPEMDRINRAFIELMLEGDASGRGF"
     gene            1352..2176
                     /locus_tag="PLQ30_08420"
     CDS             1352..2176
                     /locus_tag="PLQ30_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPW02509.1"
                     /translation="MGIVPEKKPNPSIKRVIGVISGKGGVGKSMVASLLAQAFAERGL
                     QVAVLDADITGPSIPRLLGIDSFRGESDGEHLFPVENEEGIKVLSINLFNAQEDEPVI
                     WRGPLLAKAIDQFWSDTIWGELDYLIIDFPPGTSDVALTAFQTIPFSGLVVVATPQDY
                     VSMIVRKSINMASMLKAPVLGVVENMRTMICPHCGTEVALFDDGSQQGAQRMGLPILA
                     SLPWRKELAQTRALRWSTLSETVKKDADRLAGEVELALASVSSQPKDAESEIASKE"
BASE COUNT          630 a          553 c          551 g          665 t
ORIGIN      
        1 gaaagcctct gcccgatgca tctccttcga gcatgagttc gataaaagcc ctgttgatcc
       61 tatccatttc tggcttgcaa tcgccataag agaaatcaag ctcctttcct cctacaacgg
      121 ctggtttgtc acgcaggtct tccggtacgg tccagtccag cgtgatattc gtaaaaggag
      181 cctgactccc ccagcgtgat ggcgtattaa ccccaaagac aaaactctgt aggctctgct
      241 ttatttcctt gtcgctaagg ctgtcggctc tcacaaaagg ggcgagatag gtatcaaagg
      301 agctaaaggc ctgtgctcct gcccattcat tctgaagaat accaagaaaa ttcaccattt
      361 gattggctag agtggaaaga tgactcgctg gctttgagga aatcttatcg ggaataccgc
      421 ccagcccttc gcggatcaga tctctaagag accagcctgc gcagtaaccc gaaaacatag
      481 aaagatcatg aatatgaaaa tcagcatttc gatgagcttc agcgatatcc ttaggatata
      541 tctgtgtgag ccaataattt gcggttacac tgcccgaatt atgtaagatc agtccaccaa
      601 gagaataatt gacattggca ttctcattga cgcgccagtc cgatcgagct agatatccgt
      661 tcatagttgc cgaaatgtcc aacatcagct tttttgtatc gcgaatcgct tcatgccggg
      721 ctctgtagag gatgtaggcc tttgctactg cggtttctcc ctgttctata agaacggttt
      781 cgactagatc ttgaatttct tcgatcgctg gtatggagtt aggatggcga gtctggatga
      841 gttctctaat cttcttttcc acgaggtctg tatataggat aggcttgcta ttcccctgat
      901 tatcaaggat tgatgtattc gttgcaataa atgctttttc tatggctttc tctattttat
      961 gcctatcgta actcaccact tctccagagc gcttaaccac gctctttatt gggggagcca
     1021 cttgccatcc tagaaaatca tgcccaaaaa gactgggtat ctcggtctta tctgtcatat
     1081 ctcgatcacc tctactatat attgtgtacc atgctaccaa agacacaata tatattggtc
     1141 atttggtgag cacaagaggc aaaaagtagt tgcagctatt aaacgttagt gttacgaacc
     1201 ttgtatatga agctgcaatc aaatagtcat tcattcgttt tatctagatt tctgataaag
     1261 caatgcggag gcttgaattt ttctcgatat ctacggtatt attagcatat gctcgaaact
     1321 atacaacaag attttgcata aggagtcttt tatgggcatt gtccctgaaa aaaagcctaa
     1381 tccctcgata aagcgggtta tcggtgtaat aagtggtaag ggtggcgttg gcaaatcgat
     1441 ggtcgcttcg ctgcttgcac aggcatttgc agagcgaggt ttgcaggtag ctgtgctcga
     1501 tgcagatatc acaggcccaa gcattccgcg ccttttgggt atcgatagct ttcgcggtga
     1561 gtccgatggc gaacatctct tccctgtaga aaatgaagaa gggatcaagg tgctctcaat
     1621 caatctcttt aatgcacaag aagatgaacc agtgatctgg cgcggaccat tgctggctaa
     1681 ggcgatcgac caattctggt ctgatacgat ctggggagag ctcgactatc tcattataga
     1741 ttttccgcca ggaacctcgg atgtcgcgct caccgcgttt cagactattc ctttttctgg
     1801 cctcgtcgtt gtcgcaacgc cccaggatta tgtttccatg atcgtgcgca agtcgatcaa
     1861 tatggcatcg atgctcaagg cgccggtgct tggcgtggtg gagaacatgc gcaccatgat
     1921 ctgtccccac tgcggcacag aagttgcctt attcgacgat ggaagccagc aaggtgcgca
     1981 gcgtatgggt ttgccaatcc tcgcaagtct tccttggaga aaagagcttg cgcaaacgcg
     2041 agcgctccga tggtctactc ttagtgaaac tgtaaaaaaa gacgcagatc gccttgctgg
     2101 cgaagtcgag cttgcgttag cttctgtttc gtctcaaccc aaagacgcag agagcgagat
     2161 cgcttcaaag gaataatcat gccttgtcct cgcagcgtcc aaaaaatcag ctcagaaatt
     2221 cgctcttgtt tggtaaactc ttttataaga aggagaaaca ggtagaatga aaatcgcatt
     2281 tgtatccgac gacggaatca ccatttctcg ccattttggc agagcaacat attatgtagt
     2341 tgtggaaatt gagaatggca ccatatctca caaagaaata atcccaaaat cagggcata
//
LOCUS       DAOMZL010000161         2386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_45697_length_2386_cov_12.931360, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000161 DAOMZL010000000
VERSION     DAOMZL010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45697_length_2386_cov_12.931360"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..2161)
                     /locus_tag="PLQ30_08425"
     CDS             complement(47..2161)
                     /locus_tag="PLQ30_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPW02510.1"
                     /translation="MDSSKTPGGSLRAPIRNIGILAHIDAGKTSITERILHVTGVREK
                     AGNVDDGTTTTDYLTVERQHGITIKTAAVRFVHKGVLFNLLDTPGHVDFSAEVDRVLR
                     VLDGAVIILCAVSGVQVRTGFIATGCRAHHIPRIYFINKMDRRGADFFSAVKEIAGEL
                     GEPVLPLQIPTFEDYHWNGIVDLIDMSWYPVLSNEEENESENKGDKIATASSEHHFPI
                     EKAPITEEMRRLALESREKLLDRVSMYDDSLMEKLISGRTVSEKEIRSALIAPIWKQE
                     VTPILCGSSFSDISSRLLMDAIIDYLPDPYSRGCPPAKDMKTGKEIRLEAKPDAPFSA
                     YIFKTAFDADLNTLAWIRLWSGQLREGMKVMAMPFKKIAQMKHLYGINGAEIEKIDSA
                     AAGEIVGACLSYMEAGSTLCETHLQLVYESFKNPEPLVSIALEPTSQQDLPRLRNALK
                     RFSLEDAGLLIDEEKETGRITIAGQGELHLDIVLERLQEEYGLRVRAGNPQVPKVERL
                     MKPVHIEEEFTGDFGGEKLSVTLGISLENQKDKDENKVIFAQGLRLLANFEAALHRAA
                     ETSLAVGPSGGWPIIGARLIIERITAPMGGDRRAETAVEVATSAILRKAVSLAGSYIL
                     VPIVQVSVEVPDAWFGAALASLQARGARIEAVEDEGVVKSIIAYAPMENFFGYTTSLR
                     SITEGRGIYQAKFDHYGNMNEY"
BASE COUNT          603 a          600 c          527 g          656 t
ORIGIN      
        1 aatatatgtg cctatatgtc aggaaatatg gatatagaaa tccatctcaa tattcgttca
       61 tattgccata atgatcgaat tttgcttggt aaatgccgcg cccttccgta atggatctca
      121 aagaggtggt atatccaaag aagttttcca taggtgcata ggcaataatg gatttgacga
      181 ctccctcatc ctccacagct tctatgcggg ctccccgagc ctgcaatgaa gccagggcag
      241 ctccaaacca cgcatccggt acttcgacag aaacctggac gattggtaca agtatatagc
      301 taccagcaag gctcactgcc ttgcgcagaa tcgcagatgt cgcaacttcc actgcggttt
      361 ctgctcgtct atcgccgccc atgggagctg taatccgttc aataatcagt ctagccccaa
      421 taattggcca gccaccggat ggtccaacgg ctaaagaagt ctctgctgcc cgatgcagag
      481 ctgcctcgaa gttagccaag aggcgcaatc cctgtgcaaa aatgaccttg ttttcgtcct
      541 tgtctttctg gttttccaat gaaattccta gggtcaccga aagtttttcg ccaccaaaat
      601 ctcctgtgaa ttcttcttcg atatgaacag gtttcatgag tcgctcgacc ttaggaacct
      661 ggggatttcc cgcccttaca cggagtccgt attcttcttg cagacgctcg agtacgatgt
      721 caaggtgtaa ttctccttga cctgctattg ttattcgacc ggtttccttt tcttcatcga
      781 tcaatagacc tgcatcttca aggctaaatc gcttaagggc attacgcagt cttggaagat
      841 cttgctggga agttggttcc agcgctatgg ataccagagg ttcgggattt ttgaaagatt
      901 catagacgag ctgaagatga gtctcacaga gagtactacc tgcttccata taggacagac
      961 atgcgcctac tatttccccc gcagccgctg aatcgatctt ctcgatttct gctccgttta
     1021 tcccatagag gtgcttcatc tgagcaatct ttttgaaggg cattgccata actttcatgc
     1081 cctcgcgaag ctggcccgac catagacgaa tccatgccag ggtgttgaga tcggcatcaa
     1141 aggctgtctt gaaaatataa gctgaaaatg gagcgtcagg tttagcttcc agtctgatct
     1201 ctttcccggt tttcatatcc ttcgcaggcg gacagccacg agaataagga tcaggaagat
     1261 aatcgattat cgcatccata agaagccggc tggaaatgtc ggaaaaggaa gaaccgcata
     1321 gaattggtgt cacttcctgc ttccagatgg gtgcaataag cgcggatcgg atttcctttt
     1381 cagagacggt tctcccgctt atcagttttt ccatcaggga atcatcatac atgctgacac
     1441 gatcgagaag cttttctcga gattctaggg caaggcggcg catctcctca gtaataggag
     1501 ctttttctat aggaaaatga tgttcgcttg atgcggtcgc aatcttatcg cctttgtttt
     1561 ctgactcgtt ctcttcttcg ttcgacaaaa caggatacca ggacatatcg atcagatcca
     1621 caatcccatt ccagtgataa tcttcgaagg tgggtatctg gaggggcaaa acaggttcac
     1681 cgagttcacc tgctatctct tttacagcgc tgaaaaaatc agcgccccgc ctatccatct
     1741 tgtttatgaa atagattcgc gggatatggt gagctcggca gccagtagct ataaagccag
     1801 tcctaacctg aacaccggat accgcacaaa gaatgatcac cgcaccatca agaacacgga
     1861 gcaccctgtc cacctcagca ctaaaatcca cgtgtcctgg agtatccaac agattaaaga
     1921 ggactccttt atggacaaag cgcacagccg ctgttttaat tgttattccg tgttggcgtt
     1981 caacggtcaa atagtcagta gtcgttgtgc catcgtcgac atttccggcc ttttcacgca
     2041 ctcctgtgac atgcagaata cgctcggtaa tcgatgtctt gccggcatct atatgggcga
     2101 gaatacctat atttctgata ggcgccctga ggctcccccc aggcgtcttt gatgaatcca
     2161 ttgcaatatc aatatatcag agctattaca aagataaaag cattactgta aggatgggta
     2221 cacgcttcct atggcaaata tctgctccct accagcggta gtgggcaaat gctttattag
     2281 cttaacggct gagcttcgct caacctatag cacgcttttg cgctttacca tctgtaatgc
     2341 gcaaaagctt tattcgcttc agccattttg tgcatgtcgt ctttct
//
LOCUS       DAOMZL010000162         2317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_47878_length_2317_cov_15.921309, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000162 DAOMZL010000000
VERSION     DAOMZL010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47878_length_2317_cov_15.921309"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1797)
                     /locus_tag="PLQ30_08430"
     CDS             complement(<1..1797)
                     /locus_tag="PLQ30_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02511.1"
                     /translation="MRLVARKHNLDTRYSALVFCMALIFSAVSREANAQENAAWNVVK
                     VKSGLQGNLQVQKPIHAADLFFPGDDEYDRAFSAAIRQGRLPKTNVFPLFGGELGASL
                     EAEIAEPELLSGIVFRASTPTLYYDSPAKQKDLGTSLLEDPSLDVNTFPEILHFWQTL
                     PDVLALGLNLYPSSSIAFKIDISFNYRRDKIVGQSSPFQWDINDLRENLTSAAQFPRR
                     TYISWVGKGYGVAMGRFPSGMGWGMLSGSILNPRASWYDQIRFYFESGQWRFTSMTAT
                     SSAQLSKAEQEIQFRKNSDGSSFWDSVSDHDHASHDMAIKLANWHQIEWKPAPWLELS
                     VAEMSVIGGRMPSLSFVLPTTIWHNTYAAGYSNVGVSLSAAIVPFNGLMLSGELFIDD
                     LKGAREKQSADSKPQSLAWLGTARWSALLFDTISLDTGLEYQHVDRWTYVRWSPYLAM
                     YQRQTLPDGESGQDQSLGAPWGPDYDSIGAYTSMKFKSGAYLRLSYEFIRKGPIYQGM
                     ASQTSVSADFDGDGTIDSAQQKIWVPVYYDYDEYAGDGALEAILSKPDEYRHLVSLMG
                     AVPLAKSIELQLSATLGLYRNFGHVAGASEEAF"
     gene            complement(1842..>2317)
                     /locus_tag="PLQ30_08435"
     CDS             complement(1842..>2317)
                     /locus_tag="PLQ30_08435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW02512.1"
                     /translation="ILRPSSIISCQPPSSKDAVVYRKPYLNYHNFGYICALMLNERPL
                     AVHWGLSCLQRLYYLMPAFDIEKYPSLSTGNLLLVKLLQYCAGNNYRLVDFANGDEPY
                     KEKWCEMELALYEGYIPSEPAGWLISLFESALESIKRSTLAPHLKRMAQFLKFSA"
BASE COUNT          585 a          579 c          574 g          579 t
ORIGIN      
        1 aaaagcctct tcgctagcgc cagcgacatg cccaaaattg cgatataacc ccaaggtcgc
       61 ggatagctgc agttcaatgg actttgcaag gggcactgct cccatgagcg atacaagatg
      121 gcgatattcg tcaggctttg agagtattgc ttccagggca ccatctcctg catattcgtc
      181 gtagtcatag tagacaggca cccagatttt ttgctgcgcc gagtctattg ttccgtcacc
      241 atcgaagtcg gcacttacgc tcgtctggct tgccattccc tggtaaatgg gcccttttcg
      301 gatgaattcg taggagaggc gtagataggc gccgcttttg aacttcatgc ttgtatatgc
      361 gcctatgcta tcataatcag gaccccaggg agctccgagc gattggtctt ggccagattc
      421 tccatctgga agcgtttggc gttggtacat ggccagataa gggctccagc gcacataggt
      481 ccagcgatcc acatgttgat actcaagacc agtatcaaga gaaatcgtgt caaaaagcaa
      541 tgccgaccaa cgtgccgttc caagccaggc aagggattga ggcttggaat ccgcagattg
      601 tttttccctt gcgcccttca gatcatcaat aaaaagctct ccagatagca ttagcccgtt
      661 aaaaggcact atggctgcgc tcagcgagac gcccacattg gagtatccag ccgcataggt
      721 gttgtgccag attgtggtgg gcaaaacaaa gctgagagag ggcattcttc cgccgatcac
      781 gctcatctcc gcaaccgaaa gctctaacca cggcgctggc ttccattcga tctgatgcca
      841 gttggccagc tttattgcca tatcgtgcga cgcatggtcg tggtcgctaa ctgagtccca
      901 aaaactgctt ccatcggaat tttttctgaa ttgaatttcc tgctcagcct tggagagctg
      961 ggccgagctt gtcgctgtca tactcgtaaa gcgccattga ccggattcga aatagaagcg
     1021 aatttggtcg taccaggaag ctctcggatt caagatcgaa ccggacagca taccccagcc
     1081 cataccagaa ggaaatctgc ccatggccac accatagcct ttgcccaccc agcttatata
     1141 ggttctccgc ggaaattgcg cagcagatgt aaggttttcg cgcagatcat taatgtccca
     1201 ttgaaatggc gagctctggc ctactatttt gtcacgtctg tagttgaacg aaatatcgat
     1261 tttgaatgcg atcgaggacg aagggtacag attcaaacca agcgctagca catccggcaa
     1321 tgtctgccaa aaatgcagga tttccggaaa ggtattgaca tctaggcttg gatcctcaag
     1381 aaggcttgtg ccgaggtcct tctgctttgc tggcgaatcg taatataggg ttggcgtgct
     1441 tgccctgaag acaatgcccg aaagaagctc aggctccgct atctcggctt ccaaggaagc
     1501 cccaagctca ccaccaaaga gggggaaaac attggtctta ggtaggcggc cctggcgtat
     1561 agcggctgaa aaagccctgt catattcatc atcgccagga aagaaaagat cggcagcatg
     1621 gatgggcttc tggacttgca gattgccctg tagacctgat tttactttga cgacattcca
     1681 ggcagcgttc tcttgtgcat ttgcttctcg actaacagcg ctaaagatca gcgccatgca
     1741 aaaaaccagg gcagaatacc tagtatccaa gttgtgtttt cttgctacta accgcattgt
     1801 atcccttctc tattacttta ttcagtgctt ggatctctgg atcatgctga gaattttaga
     1861 aattgtgcca ttcttttgag gtggggtgca agagtgcttc gctttatgct ctccaaagcg
     1921 ctctcaaata gggaaataag ccatccggct ggttcggatg ggatatagcc ttcatatagg
     1981 gctagctcca tctcgcacca cttctcctta tagggttcat caccattggc aaaatcgacg
     2041 agccgataat tatttcccgc gcaatactgc aagagcttga caagaagcag gttgcctgtc
     2101 gacagcgaag gatatttctc aatatcgaag gcaggcataa ggtaatatag gcgctgcaga
     2161 cagctaagcc cccaatgcac ggctagaggc ctttcgttca gcatgagcgc gcatatgtaa
     2221 ccgaagttat gataattgag ataaggtttt cggtatacta cagcatcctt cgacgaagga
     2281 ggttggcagc tgataattga actgggacgc aagatcc
//
LOCUS       DAOMZL010000163         2261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_49731_length_2261_cov_13.655938, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000163 DAOMZL010000000
VERSION     DAOMZL010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49731_length_2261_cov_13.655938"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PLQ30_08440"
     CDS             complement(<1..520)
                     /locus_tag="PLQ30_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPW02513.1"
                     /translation="MKAIRKAMLVLAFVMLLVPAFAKAGQSIVVYSSVDEVNAKKILD
                     AFTAQTGIAVQFVHLSSGPAYARIKAESANPQADVWFGAPSENHIVAKNDKLTQPYKG
                     PNFDKLGKEFKDPEGYWRSFYMNPMAFAVNKEVIARIGAPKPTSWADLLNPAYKGQIQ
                     MPSPQSSGTAYNI"
     gene            708..2021
                     /locus_tag="PLQ30_08445"
     CDS             708..2021
                     /locus_tag="PLQ30_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HPW02514.1"
                     /translation="MSNQKAQVSQKGSKAAYRWDMVFAGAMLMLCFGTVYAWSYFQVP
                     LMQTFGWNNAQVSLVFSLAIFFLGMAAAVGGVILPNAGPQRIALIGSVLFSLGYLLAA
                     FALSLKSLALLYIGYSIIGGTGLGLGYVTPVATVSKWFPEKKGLATGMVIMGFGFGAL
                     FMSKVIAPVFLRLYDGNLVNSFLTFALLFAAITIPSSLAMKDPPERALGQSKQPNSSN
                     SRRQKQDDAKTAISLSLRQASKDILSKRFLVVWMILFCNISAGISIIGFQSPMFQELI
                     INRNPSSTAEALASMGATLIAVTSIFNGVGRLLWGIFSDRFGRIRAYRLLLGSEILVF
                     FLLIFTRSPLVFAICACWILFCYGGGFGMMPATISELFGPQKMAIMYGAVLTAWSFGG
                     VIGPQITAYIKDTQPSQAIKISFIVGAVFIAIAFLFSILITDKKK"
     gene            complement(2051..2123)
                     /locus_tag="PLQ30_08450"
     tRNA            complement(2051..2123)
                     /locus_tag="PLQ30_08450"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2088..2090),aa:Met,seq:cat)
BASE COUNT          587 a          501 c          502 g          671 t
ORIGIN      
        1 cgatattata tgcggttcca gaagattgag gagatggcat ctgtatttgc cccttgtacg
       61 ctgggttgag caaatcagcc caactcgtgg gttttggcgc tcctatgcga gcaataactt
      121 ccttattcac cgcaaaagcc atgggattca tgtagaagct acgccagtat ccctccggat
      181 ccttgaactc cttacccagt ttgtcaaaat tcggcccttt gtatggctga gtgagcttat
      241 cattctttgc cacaatatga ttttcgctag gagcaccgaa ccacacatcc gcttgaggat
      301 ttgcggattc agccttgatt ctggcataag ctgggccaga agatagatga acaaactgca
      361 ccgcgatacc ggtctgggca gtaaaggcat ccagaatctt tttcgcattc acctcatcta
      421 cgctcgaata cactactatg gactgaccag cctttgcaaa ggccggtact aacaacataa
      481 caaatgccaa gactagcatt gccttgcgaa ttgctttcat aggatcgcct ccagattagg
      541 aatataatct catattttat aatcttttta ctgtgtccgc aatgagaaag agcctattta
      601 gaacacgtaa gttaggcttt cccatatcac aaccagccat ttgcttggca ttgactggac
      661 atgaattatg gcgcattctg gatggcggtt gcagaaggaa tagaatcatg tcaaatcaaa
      721 aagcacaagt ctcacaaaaa ggttctaagg ctgcatatag atgggacatg gtctttgcag
      781 gggctatgtt aatgctctgc tttgggacag tctatgcttg gagctacttc caagtgcctc
      841 ttatgcagac ctttggatgg aataatgctc aggtttcgct tgttttttcg ttggctatat
      901 tcttccttgg catggctgct gccgtaggtg gggttatact tccaaatgct ggcccccaga
      961 gaattgcact gattggaagc gttcttttta gtcttggata ccttttggca gcctttgcgc
     1021 tttcgctcaa atccttagct ttattatata taggatatag tattatagga ggcacgggac
     1081 ttgggctcgg atatgtaaca ccggtggcta cggtttccaa atggttccct gagaaaaagg
     1141 ggcttgcaac cggcatggta ataatgggat ttggcttcgg tgcgcttttt atgagcaagg
     1201 tcatagcacc agtttttctg cgattatatg atggaaatct cgtaaattct ttccttactt
     1261 ttgcattgct ttttgctgct ataacaatcc cttcatctct tgccatgaaa gacccaccag
     1321 aaagagctct tggacaatca aaacagccca attcctccaa ttctcgccga caaaagcagg
     1381 acgacgcaaa aacagcaatt tctctatctt taagacaagc ctcgaaagat attctttcaa
     1441 aaagattcct tgtggtatgg atgattctat tctgtaatat cagtgcagga atatccataa
     1501 tcggctttca atcacctatg ttccaggagc ttattataaa caggaatcct tcctcaactg
     1561 cggaagctct cgcctcgatg ggagcgactc ttattgctgt aacttctatc ttcaatggtg
     1621 taggaaggtt gctgtggggg attttctcgg acagatttgg aagaataagg gcttatcgcc
     1681 ttttgcttgg gagtgaaatc ctggtttttt tcttgctaat atttacaagg agcccattgg
     1741 tatttgcaat ctgtgcctgc tggatccttt tctgctatgg aggaggattt ggaatgatgc
     1801 ccgcaaccat ttccgagcta ttcggaccac aaaaaatggc tattatgtat ggcgccgtat
     1861 taactgcatg gtcctttggc ggagtaattg gtcctcaaat tacggcttat atcaaggata
     1921 ctcaaccatc tcaggccatc aagatttcct tcatcgtggg cgctgtgttt atcgccatag
     1981 cctttctatt ttcgattttg attaccgata aaaagaaata ggccaccttc tccaaaaaag
     2041 gcggcctatc tagcagggag aggactcgaa cctccgacct ccgggttatg agcccgacga
     2101 gctgccaact gctctaccct gcggcgcaaa aattaatcta caataaatcg caaaatatgt
     2161 caagggatag gaaaattttc tgatacaatg gtgagttttc actatcagga tttggagata
     2221 gcggcccagt ttggtttagg tcaggttagc ctaagccaaa t
//
LOCUS       DAOMZL010000164         2223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_51047_length_2223_cov_9.412362, whole genome shotgun sequence.
ACCESSION   DAOMZL010000164 DAOMZL010000000
VERSION     DAOMZL010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51047_length_2223_cov_9.412362"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLQ30_08455"
     CDS             complement(<1..337)
                     /locus_tag="PLQ30_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HPW02515.1"
                     /translation="MKADIGLVGLAVMGENLVLNMESRGFTVAVYNRTVEKVDSFLQG
                     RGAAKRILGAHSPRELIELLKKPRIVMLMVRAGMAVDEMIAQLIPFLEPGDIIVDGGN
                     SNYEDTMRRV"
     gene            complement(435..1286)
                     /locus_tag="PLQ30_08460"
     CDS             complement(435..1286)
                     /locus_tag="PLQ30_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HPW02516.1"
                     /translation="MERSTAPSCLYLIHSLSNSLSERDKRIADYILNDPAKAVHLSIE
                     ELAEAALVSVSTLVRFVKKLGFKGYQQFRIALASEALAPEARIYETIVDSGEDPVHLA
                     FSSASRALEITSSMIDGQDLNELASRIIRANCVYLFGLGGSLIVAKDALHKLVRTGVR
                     CIGAEDYHMQLMIASQMNQQDTAIVVSHTGANKDAIGLAETIKATGAFLCVITTYPRS
                     ALSRMADMRFISASSGSQAISEAFSARIAQLALIDSLYIAIMKQLGEEGIKNVEKMRA
                     TIAKRRI"
     gene            1510..>2223
                     /locus_tag="PLQ30_08465"
     CDS             1510..>2223
                     /locus_tag="PLQ30_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPW02517.1"
                     /translation="MKRKFMLVLVALLALFSASAQQKEIRVLLANHPYGDLLKLAIPE
                     YEKATGVKVNVESLQESQLTTKLTTEFATKSSTVDVFMTRPLQEGKMFYKNGWYEPLT
                     GYDFSDYPKNAMDVASFGNKAYIVPLVTEWEVLYYRIDLLKKAGISVPTNFAELEAAA
                     KKLHSGDIVGFASRGKGAAAVTQLSSYVYNYGGAYLDKGKAVFDSKEALEAIRFYGRM
                     LGNYGPAGVTNMSWENIMPL"
BASE COUNT          600 a          520 c          498 g          605 t
ORIGIN      
        1 aaacgcgcct catcgtatcc tcataatttg agtttcctcc atcgacgatg atgtcgccgg
       61 gctccaggaa tggtattaac tgcgcgatca tttcatctac agccatccca gctcgtacca
      121 tgagcattac aattctgggt ttcttaagca gttctatgag ctctcttgga gaatgagcac
      181 ccagaattcg ctttgccgct cctctgcctt ggaggaaaga atccactttt tcaactgttc
      241 ggttgtagac tgctactgta aaaccgcggc tttccatgtt gagcacaaga ttttcaccca
      301 tcaccgcaag tccaactagg cctatgtcgg ctttcataca tcctcccata tctcccctat
      361 gattagatat tcccctgtga ttagatgcgc ccctatgatc agatacgccg ctatgatcag
      421 atacgccgct atgatcagat gcgccgcttt gcgatggtag cgcgcatttt ttctacattc
      481 tttatgcctt cctctcccaa ctgcttcatt attgcgatat agagggaatc tattagagca
      541 agctgtgcga ttctggcaga gaaggcttcc gatatggcct gagaccccga ggatgccgat
      601 ataaagcgca tatcagccat cctagaaaga gcagaccgtg gataagtagt aatcacacat
      661 agaaaagctc ccgttgcctt gattgtctcg gcaagtccaa tagcgtcttt attggctccg
      721 gtatgcgata ctacgattgc cgtatcctgc tggttcatct gtgaagctat cataagctgc
      781 atatgataat cctcggcgcc tatgcaacgg actcctgtgc gaacaagttt atgcaatgca
      841 tcttttgcaa ctatcaagga tccccctaga ccgaaaaggt agacgcaatt agctctaata
      901 atgcgcgagg ccaattcatt caaatcttgg ccatctatca tcgatgaagt gatttcaagc
      961 gctctcgaag cggagctgaa agccagatga actggatctt ctccactgtc gactattgtt
     1021 tcgtaaatcc ttgcctctgg agctagtgct tccgaagcca aggctattct gaattgctgg
     1081 taacccttaa agccgagttt ttttacgaag cgtacaagtg tagaaacaga aacaagggca
     1141 gcttctgcca actcttcgat actgagatgc accgctttgg ctggatcatt cagaatataa
     1201 tctgcgatgc gcttatcgcg ctcgctcaat gagtttgaga gcgaatggat tagatataga
     1261 cagctcggcg cagtggatct ttccatagag ataccatcaa gttttattat tctatatcat
     1321 atgctcggat gagtacagag gactggcaaa aatttccaat cacctgaaaa aaaattatct
     1381 tcatatgttg acaaaaggct cttttggctc atagtataaa agctgtcaaa tccgcctagc
     1441 ttgaatcaat cctcagctga agggcggata attaaagaat tgccacacat ttctatggag
     1501 gaggtcatga tgaaaagaaa attcatgctt gtcttggtgg ctctgcttgc tctttttagt
     1561 gcaagtgctc agcaaaagga aatccgagtc ttgctagcaa accaccccta tggcgatctg
     1621 cttaaattgg ccattcccga atatgaaaaa gcgacgggag tcaaggtcaa tgtggagagt
     1681 cttcaagaga gccagctcac gacaaaactg actacagaat tcgcgacaaa atcttctacg
     1741 gtagatgtct ttatgacaag acctctgcag gaaggaaaga tgttctacaa gaatggctgg
     1801 tacgaaccac tcactggata tgatttctcg gattatccga aaaatgcgat ggatgtcgca
     1861 tcatttggca acaaagctta catcgttcct ctggtcaccg agtgggaggt gctttactat
     1921 cgcatagatc tgctcaagaa agctggcatt tccgttccta caaattttgc cgagctagag
     1981 gcagcagcta aaaagcttca ttctggcgat atcgtaggtt ttgcgtctag aggaaagggt
     2041 gccgcagcgg tgactcaact ctcgagttat gtctacaact atggcggagc atatctagac
     2101 aaaggcaaag ccgttttcga cagtaaggaa gccttggagg ctattcgctt ttatggaaga
     2161 atgctgggga attatgggcc tgccggtgta accaacatgt cctgggaaaa cattatgccg
     2221 ctt
//
LOCUS       DAOMZL010000165         2202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_51831_length_2202_cov_30.857010, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000165 DAOMZL010000000
VERSION     DAOMZL010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51831_length_2202_cov_30.857010"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLQ30_08470"
     CDS             complement(<1..255)
                     /locus_tag="PLQ30_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02518.1"
                     /translation="MKKPFSGKAGDSLVLRTIIKELGQALPRMTDRPEQQTASQSNEQ
                     MHVQMPTQSAQDYQPILSAKNLSIGYRYTLPIVSGMRFDFP"
     gene            complement(221..1033)
                     /locus_tag="PLQ30_08475"
     CDS             complement(221..1033)
                     /locus_tag="PLQ30_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW02519.1"
                     /translation="MAIRYSHHKAQGSLAPHAFVRSVRLRELLCLAIICLIALPLAAQ
                     EQLVIASTSWVQAYATAAGAKNIVTIAPFELQHPPEYEMKPSDLVAVQNASLIVYSSY
                     EKFAKKLAETAQNKNIKIIAVYTDNVPSNIIAESKKIAEALDTTEEHAKWAQSFISFT
                     NDMRARVVASLPDKRVAVQAYVKTFAVWLGLDIVGTFGPGEPSPAVVLDLIKKKPAMI
                     LDNYHNPGGKALAESLGVPNVLLINFPGKDGTRTIEDVFSYNEKAILGQSWR"
     gene            complement(1319..1771)
                     /locus_tag="PLQ30_08480"
     CDS             complement(1319..1771)
                     /locus_tag="PLQ30_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HPW02520.1"
                     /translation="MENRPIIAVIDGLGGGIGCQLCARIRQAFGQRVEILALGTNSTA
                     TEKMIKAGADRGASGENVMQVSIGLAQFILGPIGIVLPDSLMGEITPTMAQSIMRASG
                     KKILLPVSQPHFALAGVSPVPLSKLIEEAVNLLVAELDESLANKPEKQ"
     gene            1875..>2202
                     /locus_tag="PLQ30_08485"
     CDS             1875..>2202
                     /locus_tag="PLQ30_08485"
                     /inference="COORDINATES: protein motif:HMM:NF015476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009044 - xylan 1,4-beta-xylosidase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 52 protein"
                     /protein_id="HPW02521.1"
                     /translation="MESNRKNIDYYSLHGIWGAYASFVLGRIGRGAGVVVGDVRPPER
                     GLFVGYRVGAGETCLLPFSSGRKYGLGVTAYLSGESSQDIDEHYKKARRFNPEEIERQ
                     IYFSGEE"
BASE COUNT          545 a          504 c          526 g          627 t
ORIGIN      
        1 tggaaaatcg aagcgcattc cgctgacaat cggtaaggtg tatcgatatc ctatggagag
       61 attctttgcc gatagtattg gctgataatc ttgtgcgctt tgtgtcggca tctgtacatg
      121 catctgctca ttgctctgtg aagcagtctg ttgttcaggc ctatctgtca ttcttggcag
      181 cgcttgacct aattctttta taattgttcg aagcactaag ctatctccag ctttgcccga
      241 gaatggcttt ttcattgtag gaaaatacat cctctatcgt gcgcgtgcca tcctttcccg
      301 ggaaatttat gaggagcaca ttcgggacac caaggctttc ggctaatgct ttgccgcccg
      361 gattgtggta gttatctaaa atcatcgcag gctttttctt tattagatcg agcacgaccg
      421 ctggcgatgg ctctccagga ccaaaggttc caacaatatc gagccccagc cacaccgcaa
      481 aggttttgac ataagcttgc acggcgaccc tcttatctgg cagtgaagca acgaccctgg
      541 ctcgcatatc attggtaaag gatatgaagg attgagccca ttttgcatgc tcttcggtgg
      601 tatccaaggc ctctgcgatt tttttcgact ctgcgatgat attcgaaggc acattgtcgg
      661 tatagacggc tattatcttg atgtttttat tttgggctgt ctcagcgagc ttctttgcaa
      721 acttctcata gctagaatag acaatcaatg aggcattctg caccgcaact aggtcggatg
      781 gtttcatttc atattcggga ggatgctgaa gctcgaatgg agcgattgtt actatatttt
      841 ttgctccggc ggctgtcgca tacgcctgca cccatgaggt ggaggcaatg acaagttgtt
      901 cctgtgcagc aagaggcaga gcaataaggc agataattgc gaggcataga agctcgcgaa
      961 ggcgtactga gcgaacaaat gcgtgtggcg ctagagagcc ctgcgcctta tgatgagaat
     1021 acctgatagc catagagaat cctctggaaa tgaagaaaat ttaggtaggt ttcgagctca
     1081 ccttagagag tgcttcgtgc acttcgattt tcaatgatgc tccaggaggc aacgcttctg
     1141 gccgcattca tttagggcca cttttaacaa ttcagccctc atccataaat tcgcaagcat
     1201 tcaatttagc tcgcattcat ttagggcaaa attccatgtg ctctggcctt cgggccagta
     1261 ggttcttacc agattattct atttaccttt ctatcatatc ttcaagtgca aatcttttct
     1321 attgcttttc tggcttattt gcaaggctct catctagctc ggcaacaaga aggttcaccg
     1381 cttcctcgat caatttcgaa agaggcacag gagatactcc ggcgagcgcg aaatgaggct
     1441 ggctcacggg gagcaatatt ttcttgccgc tcgcgcgcat tatggattgc gccatcgttg
     1501 gcgtaatctc gcccatcaag gaatcgggca atacaatgcc aatagggcca agaataaatt
     1561 gtgcaaggcc tatggaaact tgcatcacgt tttctccact tgctcctcga tcggcgccag
     1621 ccttgatcat cttttcagtt gctgtggaat tggtgccaag agccaagatt tctacgcgtt
     1681 gaccgaaggc ctgacggatt cttgcacaaa gctggcaccc gattcctcct ccaagcccat
     1741 cgattacagc aataatcggt ctattctcca taaaagaaag atagaatcaa tcgattgatt
     1801 ttgcaacaac aataaggcta tcctcataca tataggtttt tgcaattact agcaacctaa
     1861 atggaagtct atcgatggag tctaatagaa aaaatatcga ttactattcc ttgcatggta
     1921 tatggggagc ctatgcttcc ttcgttcttg ggcgtatagg gcgcggggca ggagtagtag
     1981 tcggagatgt ccgccctccc gagagaggat tgtttgttgg atatcgcgtt ggagcggggg
     2041 agacttgtct tcttcctttt tcttcgggga gaaaatatgg cttaggtgtt acagcatatc
     2101 tctctggcga atcatcccaa gatatcgatg aacattacaa gaaggccagg cgctttaatc
     2161 cggaggagat cgaacggcaa atctactttt caggcgaaga gt
//
LOCUS       DAOMZL010000166         2193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_52159_length_2193_cov_27.206735, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000166 DAOMZL010000000
VERSION     DAOMZL010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52159_length_2193_cov_27.206735"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PLQ30_08490"
     CDS             complement(<1..451)
                     /locus_tag="PLQ30_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW02522.1"
                     /translation="MAGVQLRLIGHMTVLIEMDGFTILTDPWFGPRGRKERALAPRIV
                     PPAASLGELGHIDLLLVSHNHIDHCDDISLETARDKGITVVGPRSVAKRAKKLGAKSV
                     VELEPDMVHRINKRSGDRSSQLSIEALKADHPLASDALAYLIRGSKTV"
     gene            complement(523..1359)
                     /locus_tag="PLQ30_08495"
     CDS             complement(523..1359)
                     /locus_tag="PLQ30_08495"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPW02523.1"
                     /translation="MMQAFYGTSGFKEIHQRQRACSSLYLFEGFALFLGNRIEADVKP
                     HSHHLLKIFISLDHSFKIEHRGEFREVEIAIIAPDEAHRFASDDGNYAVLYLDGESEA
                     ARYLCSKYIGSHGLCLLPELYLMPAKAELMELEDGRGSISKAECVSKSILGLLLGPYG
                     QETVELDPRIKKVIAFLKESPDKKVKIEVVARHVGLSESRLIHLFTHQVGVPPRRYAL
                     WLRLLDAIDEIVDGASFTDAAYAAGFSDSAHLSRTFRKMFGLSLVEVFKSTSVASSFK
                     RL"
     gene            complement(1356..>2193)
                     /locus_tag="PLQ30_08500"
     CDS             complement(1356..>2193)
                     /locus_tag="PLQ30_08500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW02524.1"
                     /translation="ISDIAPENQRSTVNSVATMLQVLGQLVLALASGLFLWSRNPSYV
                     FWLVAAGLVVFTTITVFGVHEKRENIQIREKLSLKQHLQLVAKNKEAQKFFIVQFLLW
                     FGVNAATPFLTLFVSTEIEGVSPALAQALAAILLGSTAIFAVPVGIIADKTSRKLILS
                     IGLGLFGLGALAAGLFAYSLPVLIPLIIIIGIGNTAHTVLSYPLLSELVPNENVGEFW
                     GLNTFFASIGALVSSTLAGALADFFGTYRAVFVLTGICMIAAMVVLQSVEPKKAQATV
                     TT"
BASE COUNT          596 a          536 c          524 g          537 t
ORIGIN      
        1 agactgtttt ggatcctctg atcaggtatg caagagcatc ggatgcaaga ggatgatcgg
       61 ccttcagtgc ttcgatcgat aattggctcg atctatctcc cgatctttta tttattctgt
      121 gtaccatgtc tggctcgagt tcaacaactg atttcgcgcc tagcttcttt gcccgcttcg
      181 ctacggagcg aggaccgacg acagtgatac ccttatctct agctgtttct agggaaatat
      241 catcgcaatg gtcaatgtgg ttgtgggaga cgaggagtag gtcgatatgg cctagttccc
      301 caagacttgc agctggtgga acaatgcgcg gagcaagtgc ccgctcttta cgccctcgcg
      361 gcccaaacca aggatctgta aggatagtga agccatccat ttcaatgagg actgtcatat
      421 gcccgatcag ccggagttga acgccggcca tagctcacct cccgagacct tttctgggca
      481 ttcgaagcct attaggtcag tgaaaagtct agcacaccct tcttaaagtc gcttgaacga
      541 acttgctaca ctggtactct tgaagacttc gacaagacta agtccaaaca tcttcctaaa
      601 agttctactg aggtgagcag agtccgaaaa tcctgccgca taagcagcat ctgtgaagga
      661 agcgccatcg acaatctcgt cgatagcatc gagaaggcga agccaaagag cataccgcct
      721 tggaggcaca ccaacttggt gagtaaaaag gtgtatgagc ctgctctctg agagacctac
      781 atgcctcgcc acgacttcta ttttgacctt cttatctgga gattccttga ggaaagcaat
      841 cactttcttg attcggggat cgagttccac tgtttcttga ccataaggcc caagcaatag
      901 acccaatatt gacttagata cgcattcagc cttagatatg gagcctcttc cgtcttctag
      961 ctccatgagc tcggccttgg ccggcataag gtataattcg ggcaagagac agagcccatg
     1021 cgaaccaatg tatttgctgc aaaggtacct ggctgcctcc gattctccat cgagatagag
     1081 gacggcatag tttccatcgt cgcttgcgaa gcgatgagct tcgtcgggag ctataatagc
     1141 tatctcgacc tctctgaatt ctccacgatg ctcgatcttg aaagaatgat ccagggatat
     1201 gaaaattttg aggagatgat gactatgtgg ctttacatcc gcttcgattc tgttgccgag
     1261 aaagagagca aacccttcga ataaatagag cgacgagcaa gcgcgctgcc gttgatgtat
     1321 ctccttgaaa cccgaggttc catagaaagc ttgcatcatg ttgtcactgt ggcttgagcc
     1381 tttttaggct cgacagattg aagaacgacc atagccgcga tcatacagat gcctgttagc
     1441 acaaagacgg ctcggtaggt cccaaaaaag tcagcgagag cccccgcaag agtggatgag
     1501 actaatgcgc ctatcgaggc aaagaaagtg ttcaggcccc agaattctcc cacattttca
     1561 tttggaacta gctccgatag cagaggatat gagaggactg tatgggctgt atttcctatt
     1621 ccgataatga tgattagtgg aatcaaaact ggcaaagaat atgcaaataa accagccgca
     1681 agtgcaccca ggccgaagag gccaagacct atcgagagaa tgagttttct gctggtttta
     1741 tctgcaatta tccctactgg cactgcgaag attgcagtcg aaccaagaag gattgccgcc
     1801 agagcctgtg ccaaggctgg agagacacct tctatttcag tgctgacaaa aagtgtaaga
     1861 aaaggcgtcg cagcattcac cccgaaccaa agcaaaaatt gaacaatgaa gaatttttga
     1921 gcctctttgt ttttcgcaac gagctgtaaa tgctgcttca gactgagttt ctctcttatc
     1981 tgaatattct cacgcttctc atgaacaccg aagaccgtga ttgtcgtaaa taccactaag
     2041 ccagcagcta caagccagaa aacataagaa ggattcctcg accaaaggaa tagcccgcta
     2101 gcaagcgcca gcacgagctg acccagtact tgtagcatgg tggcaacgct attcaccgta
     2161 gaacgctgat tctctggggc tatgtccgaa atg
//
LOCUS       DAOMZL010000167         2167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_53165_length_2167_cov_17.464015, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000167 DAOMZL010000000
VERSION     DAOMZL010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53165_length_2167_cov_17.464015"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PLQ30_08505"
     CDS             <1..663
                     /locus_tag="PLQ30_08505"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HPW02525.1"
                     /translation="QRSVFLFGPRQIGESSYVQEQLESHPVLSYNLLDGGLRLRLLDP
                     TLIRQEVEARNLPAFARFLQTAATVNAKIINYTNIANDAQVLRQTVCLWFQILIDTML
                     GYELLPYTSTLKRKAIKTAKFYFFDIGVVRILRRLPPIVPEGADFGEFFEHYIFMELK
                     AYLDYRKPSSRLSYWRSISGYKEKSSTSTSSAGNPVLEGSMVSIFCPGANSLLGYGRA
                     IL"
     gene            679..1338
                     /gene="cas4"
                     /locus_tag="PLQ30_08510"
     CDS             679..1338
                     /gene="cas4"
                     /locus_tag="PLQ30_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HPW02526.1"
                     /translation="MYSEDDLIPLSSLQHILFCERQYALIHIEQVWEENLFTAEGRVL
                     HERVDVEHRESRRLFRQEYSMAVRSLNYGLIGKCDLVEIYLAPGGTVAEAVPVEFKRG
                     RNKEEDVDRVQLCAQALCLEEMLNITIGTGQLYYLQEHRRTTVNLDGALRAKTVELIA
                     RIRQLQSSGITPSAEYQKRRCDRCSLLEVCMPKSAGSGGKEVDRFIRSQVRAAREDLD
                     Q"
     gene            1364..>2167
                     /gene="cas1c"
                     /locus_tag="PLQ30_08515"
     CDS             1364..>2167
                     /gene="cas1c"
                     /locus_tag="PLQ30_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HPW02527.1"
                     /translation="MRKLLNTLYVTSQGSYIHKEGETIIVEREQQKLLQLPVHTLGGI
                     VCFGNVLCSPFLLGFCAEHDISISFLTENGNFLASVQGPVSGNVLLRREQYRIADNPD
                     KTAKIAANIVTGKLANSRLVMNRAIRDHGDKINITAIKSASASIDRLMGALPHVVDLD
                     EIRGIEGMAASEYFAVFNQLIVDQKNDFIFNERNRRPPLDPVNALLSFVYTLLVHDVR
                     SALETVGLDPAVGFLHRDRPGRPGLALDIMEEFRPVIADRLVLSLINRRQ"
BASE COUNT          580 a          480 c          508 g          599 t
ORIGIN      
        1 cagcggtcag tatttctctt tggcccacgg cagatagggg aatcttcgta tgttcaggag
       61 caactcgagt cacatcctgt tttgagctac aacctgcttg atggaggcct tcgactacgg
      121 ctccttgatc cgactctgat acgacaagaa gtggaagcac gcaacctccc tgcctttgct
      181 cgttttctgc aaacagcagc aaccgtaaac gcaaagataa tcaactacac caacattgca
      241 aacgatgcgc aagtactgcg tcaaaccgta tgcctttggt tccagattct catcgataca
      301 atgcttggtt atgaactttt gccatataca tcgacattga agagaaaggc gatcaaaact
      361 gcaaagttct acttctttga tatcggggtc gtgcgtatat tgaggcggct ccctccaata
      421 gtgcccgaag gtgcagactt tggtgaattc ttcgagcact atattttcat ggaactcaaa
      481 gcctatctcg attaccgaaa accttcatcg cgcctttcat attggcgatc tatctcaggc
      541 tataaggaaa aatcaagcac ttctacctca tctgcaggga atcccgtcct cgaaggctcg
      601 atggtatcga tattttgtcc tggcgctaat tccttactcg gctatgggcg ggcgatatta
      661 tgacaggagg ctaatctctt gtattcagaa gatgatttga ttcctctctc ttcgctccag
      721 cacattctct tttgcgagcg gcagtatgct ttgatccata tcgagcaggt ctgggaggaa
      781 aaccttttca ccgccgaagg aagggtcctc cacgagcgag tcgatgtgga acatcgtgaa
      841 tctcgaagac tcttccgtca agaatatagt atggctgtgc gctctctaaa ctatggtttg
      901 ataggaaaat gtgatcttgt tgagatctac cttgccccag gggggactgt tgctgaggca
      961 gtgccagtag aattcaaacg cgggaggaac aaagaggagg atgtagatcg cgtacagctc
     1021 tgcgctcaag ctttatgcct agaagagatg cttaatatca caataggaac cggacagctg
     1081 tattatctgc aggagcatcg tagaacaacc gtgaatctcg atggagctct gagagcgaag
     1141 actgttgagc ttatagcaag aatacggcaa ttgcagtctt ctgggataac cccttcagcc
     1201 gaatatcaaa agcgcagatg cgatcgatgt tctcttttag aggtatgcat gccaaaaagc
     1261 gccggatctg gaggaaaaga ggtcgatcgt tttatacgct cacaggttcg cgcggcaaga
     1321 gaagatttgg atcaatgata atatgcaata gaggaaggag agagtgagaa agcttctcaa
     1381 cacactatat gtcacttccc aaggttcata tattcacaaa gaaggcgaaa ccatcatagt
     1441 tgagcgagaa caacaaaagc tccttcagtt gcctgtccac acactgggcg gtatcgtatg
     1501 ttttggtaat gtgctttgtt ctccttttct gcttggattc tgtgcagaac atgacataag
     1561 tatatcattt cttacagaga atggtaattt tcttgcttcg gtacaagggc ctgtttctgg
     1621 taatgtcctt cttcgtcgag agcaataccg catagcagat aatcccgata agacagctaa
     1681 aatcgctgcg aatatcgtaa cgggcaaact tgcgaattcg cgattggtca tgaatcgggc
     1741 tattcgcgat catggcgata agatcaatat aactgcaata aagagcgctt ctgcttctat
     1801 agataggctc atgggtgcgc ttcctcatgt tgttgattta gacgaaatac gcgggattga
     1861 aggtatggca gcctcagaat attttgccgt gttcaatcaa ttaattgtcg atcagaaaaa
     1921 tgatttcata ttcaatgaaa gaaaccgaag gcctcctctc gatcctgtca atgccctttt
     1981 atcttttgta tatactttgc tagttcatga tgtgcgatct gcgttagaga ccgtggggct
     2041 tgatccagca gttgggtttc ttcatcgaga tcgccctgga aggcctggtc ttgctcttga
     2101 catcatggaa gaattccgtc cagttattgc cgatcgtctt gtgctctctt taataaatag
     2161 aagacaa
//
LOCUS       DAOMZL010000168         2141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_54192_length_2141_cov_14.897411, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000168 DAOMZL010000000
VERSION     DAOMZL010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54192_length_2141_cov_14.897411"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            458..862
                     /locus_tag="PLQ30_08520"
     CDS             458..862
                     /locus_tag="PLQ30_08520"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPW02528.1"
                     /translation="MKIAFISDDGITISRHFGRATYYVVVEIENGTISHKEIIPKSGH
                     STFAGQHNDSHEHSALGGHGFDAVSQSKHASMLEPVRDCVAVIAGGMGAGLYANLQSM
                     GILAIVTDKLNIEEAAAAYVAGTLENHIEYLH"
     gene            complement(866..1750)
                     /locus_tag="PLQ30_08525"
     CDS             complement(866..1750)
                     /locus_tag="PLQ30_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW02529.1"
                     /translation="MIQMEFLDSLSIAVLAENSVLQGTKFLGQHGISFYLVASKNGIS
                     KHILIDTAQNTQAVLHNMALMGFQASSIDVIILTHCHYDHTDGLADMLKSIGKRDLPV
                     IAHPDLFRETFVEKPFFRSIGMNSQHSKAALEELGAHWFLTSEPLQLLPGLVTTGYVT
                     RSNDFEDPGIVSKTIDHDSRLISDPMRDDISVIAVTKDKGLIILTGCSHAGIVNIVNH
                     AIAMTGIQKVQMLIGGLHLVNASQERIEKTVDMLDSLICGSVYAGHCTGFDAEIALRY
                     RFKDRFLPLQTGMRFEIA"
     gene            1802..2083
                     /locus_tag="PLQ30_08530"
     CDS             1802..2083
                     /locus_tag="PLQ30_08530"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW02530.1"
                     /translation="MNALQETNELAQFGFSPGALDISIVYRALRGLEIEGLVSTSLDK
                     KSLGPQRRVYEITPQGSEVLSQLIEELKERKQEIEAIEAAYEAANKKHP"
BASE COUNT          627 a          460 c          472 g          582 t
ORIGIN      
        1 atgccagcaa agacacaata tatattggtc attgggcgga cgcaagaggc aaaaagcaat
       61 ggcagctata aaacatctag gaagctgcaa tcaagtagcc attcattcac tttatctaga
      121 tctctgataa agcaatacaa aggcttgaat ttttctctat atctgcaata ttattagcat
      181 atgctcgaaa ctatctatca agatttggcg taaggagtct cttatgggca tttcccctga
      241 aaaaagccca atcgcactat aaagcgattt attgctgtga gaagtggcaa gggcggcgtg
      301 gggaaatcga tggtcccttc gctgcttgcc aaggcctttg cagggtgaga tcgcttcaaa
      361 ggaataagca tgcctcgtcc gcgcagcgtt cgaaaaacca tttcaaaaat tcaatcttgt
      421 ttggtaagct cttttataag aaggagaaac atgcattatg aaaatcgcat ttatttccga
      481 cgatggaatc actatttctc gccattttgg cagagcaaca tattatgtag ttgtagaaat
      541 tgagaatggc accatatctc acaaagaaat aatcccaaaa tcagggcata gtacatttgc
      601 tggacagcat aatgattcgc atgaacatag cgcgctcggt ggccatggtt ttgatgcagt
      661 atcccaatcc aaacatgcga gcatgctcga gcctgtccgc gattgtgtgg ccgtcattgc
      721 cggaggtatg ggtgctgggc tttatgccaa tcttcaaagc atgggcattc tagccattgt
      781 tacagacaag ctcaatatcg aggaagccgc tgcagcctat gtcgcgggaa ctttggaaaa
      841 ccacattgaa tatctccact aaggttcatg caatttcaaa tcgcatccct gtttgcagag
      901 gtagaaatcg gtctttaaat ctgtagcgta gcgcaatctc tgcatcaaag cccgtacaat
      961 gacctgcgta tactgatccg catattaagc tatcgagcat atcgactgtt ttctcaattc
     1021 tttcttgaga agcattgaca agatgaagtc cgcctatcaa catctggact ttctgtatgc
     1081 ctgtcattgc gatagcatga ttcacaatat taacaatgcc tgcatggctg catcctgtaa
     1141 ggattatgag ccccttatcc ttagtgacag cgatgactga gatatcgtct ctcatgggat
     1201 ctgaaatcaa tcgagaatca tgatcaattg tcttcgaaac aattccagga tcctcgaaat
     1261 cattcgatcg agtaacatac ccggtcgtca ctaagcccgg tagcagctgt aatggctcgc
     1321 tcgtgagaaa ccagtgtgca cctaattctt caagagctgc tttgctatgt tgagaattca
     1381 ttcctattga tctaaaaaag ggcttttcga caaaggtttc tcgaaatagg tctgggtgtg
     1441 caataacagg caaatccctc ttgccgatgc tcttcagcat gtcggctagt ccatcagtat
     1501 gatcgtagtg gcaatgtgtc agaataatca catcgatgct cgaagcctga aagcccataa
     1561 gcgccatatt gtggagtact gcttgggtat tttgtgcggt atcgatgagg atatgttttg
     1621 atatgccatt ctttgacgca accaaataga aagatatgcc atgctggccc agaaacttag
     1681 tcccttgcag cactgaattt tctgccagaa cagcaataga cagagagtcg agaaattcca
     1741 tttgtatcat caactatctc cttcttaatt aaatgcttgg tttttacatt atgaaaagat
     1801 catgaacgct ttacaagaga caaacgaact cgcccaattc ggcttttctc caggggcact
     1861 cgatatcagc attgtctatc gggcattgcg cgggctcgag atcgaaggac tcgtatcaac
     1921 atcgttggac aaaaagagtc taggccctca gcgaagggta tatgaaataa ctccacaagg
     1981 gtcagaagtg ctttcgcagt tgattgaaga actcaaggaa agaaaacagg aaatcgaagc
     2041 gattgaagct gcctatgaag cagcaaataa aaagcatccg tagcgattgc caattaaaat
     2101 tttggaggta tgattatgcc gtatcttgat ggtactgggc c
//
LOCUS       DAOMZL010000169         2118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_55094_length_2118_cov_14.707707, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000169 DAOMZL010000000
VERSION     DAOMZL010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55094_length_2118_cov_14.707707"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PLQ30_08535"
     CDS             complement(<1..310)
                     /locus_tag="PLQ30_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HPW02531.1"
                     /translation="MDFILDLIGRSKELFAADIATHQKELAELIQGSRFLVIGGAGSI
                     GQAVTKEIFARSPKLLHVVDISENNMVELVRDIRSSLGYIEGEFATFAIDAGSDIFDA
                     F"
     gene            complement(346..909)
                     /locus_tag="PLQ30_08540"
     CDS             complement(346..909)
                     /locus_tag="PLQ30_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02532.1"
                     /translation="MPSPSGNISLVFSLGKFEIEGKTKVIDRIVIEERRIIVTIGGSS
                     DDARMLMKDLLSLIASFETRHEVRPIMPVIEIDESQSTVELAIDFHRLLAGSISANIS
                     EVLGSLTNVAPPSMRAAITPSALRFKIRYLGENEDLRKHSISMADKYVSIEAREGIGI
                     DARIFFASAPVKSDSLLELLRYFENGA"
     gene            complement(948..1346)
                     /locus_tag="PLQ30_08545"
     CDS             complement(948..1346)
                     /locus_tag="PLQ30_08545"
                     /inference="COORDINATES: protein motif:HMM:NF017355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HPW02533.1"
                     /translation="MTKDIIEKYEYHIFFSDEDDTYIATVAEFPYLSSFDDTPEGAYK
                     NIKDVVKEAVEVLQEERKELPIPLVDREFKGNISLRLSPETHRMAAQRAREEGCSLNQ
                     FLTSLIERNLYSNSIFTAVEQLTQMISILS"
     gene            complement(1699..2022)
                     /locus_tag="PLQ30_08550"
     CDS             complement(1699..2022)
                     /locus_tag="PLQ30_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HPW02534.1"
                     /translation="MDAILNLIGLDADLFAADIASHEEALSDLVRSSRFLVIGGAGSI
                     GQAVTKEIFARSPKLLHVVDISENNMVELVRDIRSLLGYIEGELREQKLRRQGHQWSE
                     LCRKF"
BASE COUNT          565 a          501 c          442 g          610 t
ORIGIN      
        1 taaacgcatc gaagatatcg ctccccgcgt cgatggcaaa ggtggcaaat tcgccttcga
       61 tatacccaag cgacgagcgt atatcgcgca cgagctcgac catgttgttc tcggagatat
      121 cgaccacgtg gaggagtttt gggcttcggg caaaaatctc tttggtgacc gcctggccga
      181 tcgaacccgc accgcctatg actaaaaagc gcgagccctg gatgagttcg gcaagttctt
      241 tttggtgagt ggcgatgtcg gcggcaaaaa gctcctttga gcgccctatg agatcaagta
      301 taaaatccat gattaccgct ttcaaaatca aatttaaaat tggggtcagg ccccgttttc
      361 aaaatatcgc agcaattcca acaaagaatc agattttaca ggagctgatg caaaaaatat
      421 ccgcgcatcg ataccgattc cttcacgggc ctcaattgat acatatttat cggccattga
      481 gattgagtgt tttctcaaat cttcattttc tcccagatag cgtatcttga atcgcaaagc
      541 ggatggtgta atggcagctc gcatcgaagg tggagcaaca tttgtcaacg agcctaatac
      601 ttcggagata tttgctgaaa tcgaacctgc cagcagacga tgaaaatcaa tcgcaagctc
      661 cactgtagat tgtgactcat cgatctcgat gaccggcatg atcgggcgaa cttcatgcct
      721 tgtctcgaat gaggctatca gagagagcaa atctttcatc agcatccttg cgtcatctga
      781 actaccgcca attgtgacga taatccttct ttcttctatt acgattctgt cgattacttt
      841 tgttttccct tctatctcga attttccaag agaaaaaaca agactaatat tccctgatgg
      901 tgagggcata ttcaatttcg agcaaccata tttctgaatg atcgcattta agaaagaatt
      961 gatatcatct gagtaagttg ttcgactgca gtaaaaatgg aattggagta gaggttccgt
     1021 tcaatcagcg atgttagaaa ttgattcagg gaacatcctt cttcgcgggc cctctgcgca
     1081 gccattcgat gagtctcagg tgataagcgc aatgaaatat tacctttgaa ctctctatcg
     1141 acaagaggaa tcggtagctc tttcctttct tcctgcaaaa cttcgactgc ttctttgacg
     1201 acatccttta tattcttata cgcgccttct ggtgtatcat caaaggaact taagtatgga
     1261 aactctgcca ccgtagcaat ataggtatca tcttcatcag agaaaaaaat atgatactca
     1321 tacttctcaa tgatatcttt tgtcattctt cacctcgtag tcttaacttg agtagctccg
     1381 caatctgtct ctgctgatac agtttagcat ctcccgactt ccccgattgg atattgacaa
     1441 tcgaacctgt tgaattatca aaatagactc tatgactcgt tgctttctgt ctctttaggc
     1501 tgaagaacct gtcgcaaatc gaacacaatt cctcgtattt gagaggcttc ttctcaagat
     1561 gatcaatgat acgatttagg tcccattgtc ccacgacttc tacccctcat aatagcacta
     1621 ttgatagcac tcagcaaggc ccttaagacg attccgatct atccttcttc tatcacgctc
     1681 cttggataag gttttgattc aaaactttct gcataattcg ctccattgat gtccttgacg
     1741 cctcagcttc tgttcccgta attcgccctc gatataccca agcaacgagc gaatatcgcg
     1801 cacgagctcg accatgttgt tctcggagat atcgaccacg tggaggagct ttgggcttcg
     1861 ggcaaagatt tctttggtga ctgcctggcc gatcgagccg gctccgccaa tgactaaaaa
     1921 gcgcgagctc cggacaaggt ctgaaagcgc ttcttcgtgg ctagcgatgt cggcggcaaa
     1981 taaatcagca tcgagaccaa taaggtttag gattgcatcc ataatgtttt tgacgagatt
     2041 gccgagataa tgtttctgac agaattacag gattgtactg gatttgctga aattcttcat
     2101 gcatagtatt ccgctatc
//
LOCUS       DAOMZL010000170         2116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_55203_length_2116_cov_19.375546, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000170 DAOMZL010000000
VERSION     DAOMZL010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55203_length_2116_cov_19.375546"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1523
                     /locus_tag="PLQ30_08555"
     CDS             <1..1523
                     /locus_tag="PLQ30_08555"
                     /inference="COORDINATES: protein motif:HMM:NF023591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02535.1"
                     /translation="VPATIKSRCQQFNFRLISVDIIFELLKQVALETGIEAEEEALLW
                     IAREAAGSIRDAYTLFDQIASFSGTKITAQNIRETLGLVGLDSLNALFRAIIKGNSKE
                     AFVILDEILMQGVSPEQLLTDAVNYCRSVLLIMNDIKKEDLIIAPRAAFEPIVIESLS
                     KSQVEYSIALLLDAYRHLKESIEPRHEVELAIAKLCHLSSYISPHELFDMLHNIQRTF
                     KTNEMHALSLNENKSSELLINKTTNNESSDRQILLSELRKKIIGKLRNQNLFLASALD
                     KSAEWQSKHNGLIIPVSNRVEMDILLRFEALIMETAAQVLGKPCAIEVRYLSGQESSE
                     NSENCNLRPQLQPKTKSNEPRSQTPVPHDCETIQGEIDQKKVANERPPKKSNPSLQEN
                     MEIIGEKEVSPDSLEHTFETQQTGRSDNDEKIASTNQIQFSDNLEQNKPKESEAHVID
                     VVCKVFKGAVIGTKLSYEPSNPEKSSFAFNLSRNTSYSSELSNQSEEFTDIQDEEI"
     gene            1551..1904
                     /locus_tag="PLQ30_08560"
     CDS             1551..1904
                     /locus_tag="PLQ30_08560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPW02536.1"
                     /translation="MDLTNIFDMLKNPQALKMQAEEMQKKMNSIRATGQAGGGMVKIT
                     LSGNMELLECLISVELSDLKDIPLLQDLIRAAHHNATEKIREAIQKELAGSLGELSTG
                     IPFGDFIGNFKGTEV"
     gene            complement(1885..>2116)
                     /locus_tag="PLQ30_08565"
     CDS             complement(1885..>2116)
                     /locus_tag="PLQ30_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02537.1"
                     /translation="VRIETRSSIDFIIMSFLLAYLINLRNHQALSPKLSPKLAKAHQS
                     LKASKVQIRTWRSERERNQRMKFKKRLKPPSP"
BASE COUNT          684 a          427 c          428 g          577 t
ORIGIN      
        1 aggttcctgc aactataaaa agccgatgcc agcaattcaa tttcagactg atttcggtag
       61 atattatttt tgaactttta aagcaagtcg ctttagagac aggtatcgaa gcggaagaag
      121 aggcactatt gtggatagct cgagaagctg ctggctctat ccgcgatgct tatactcttt
      181 ttgatcaaat tgcttctttt tctggtacaa aaatcactgc ccaaaatatc agagaaacac
      241 tgggattagt agggctcgac tctctcaatg ccctttttcg agcaatcata aaaggaaact
      301 ccaaggaggc attcgttatt ctagatgaaa ttcttatgca gggtgtatct ccagaacaac
      361 tcttgacaga tgctgtaaat tactgccgtt ctgttctgct tataatgaac gacattaaga
      421 aagaggactt aataattgcg cctcgtgcag ctttcgagcc tatagttatc gaatctctct
      481 cgaaatccca agtcgagtat tctattgctc tacttctcga tgcatatcga catcttaagg
      541 aaagcataga accacgacac gaagtcgaat tggctattgc taaattgtgc cacctctcat
      601 catacattag tccgcatgag ttatttgaca tgctccacaa cattcagcgg acctttaaaa
      661 ctaatgaaat gcatgcttta agcttgaacg aaaacaaatc ttcagaatta ttaataaaca
      721 aaacgacaaa taacgaatct tccgatagac aaatactatt gtccgaatta agaaagaaaa
      781 tcattggcaa attgcgaaat cagaacctct tcctggcgtc ggctctagat aaatcagcag
      841 aatggcaatc taaacataat ggcctaatca tccctgtttc aaatcgggtt gagatggaca
      901 tccttttgcg atttgaagct ctcatcatgg agacagctgc ccaagtgctg ggaaaaccat
      961 gtgccattga agtacggtat ttatcagggc aagagagcag cgagaatagc gaaaattgca
     1021 acctccgtcc ccagttacag ccaaaaacca aatcaaacga accacgatca cagactcctg
     1081 tccctcatga ttgtgaaact attcaaggag aaattgatca gaaaaaagtt gccaatgaaa
     1141 gaccccccaa aaaatcaaac ccttcattac aggaaaatat ggagataata ggtgagaagg
     1201 aggttagtcc tgactctctt gagcatactt tcgagacaca gcaaacaggg cgttcagata
     1261 atgacgagaa aatagcgagc actaatcaga ttcaattctc agataaccta gaacaaaaca
     1321 agcctaagga atcggaagct catgttatcg atgttgtatg caaggttttc aagggtgcgg
     1381 tcataggaac aaagctctct tatgagcctt ctaatcccga aaaatcgtcc tttgctttta
     1441 atctatcaag gaatacctca tattcatctg agctctctaa tcagtctgaa gaattcactg
     1501 atatacagga tgaagagatc tagatttata aggaataaat tggaggttac atggatctta
     1561 cgaatatttt cgacatgcta aagaatccac aagcattaaa aatgcaggct gaagaaatgc
     1621 aaaaaaaaat gaactcgatt cgagcgacag gacaggctgg tggcggcatg gtcaaaatta
     1681 ctctatcagg aaacatggaa ttgcttgaat gcctaatctc tgttgagctt tcggatttaa
     1741 aagatatacc gttgcttcag gatctcattc gggcagccca tcataatgct acggaaaaga
     1801 ttagagaagc catccaaaaa gaattagctg gctcgttagg agaattgagt actggcattc
     1861 catttggaga ctttattggc aatttcaagg ggacggaggt ttaagtctct tcttgaattt
     1921 catgcgctga tttctctctc gctcgcttct ccatgttcta atctggactt tgctggcctt
     1981 taggctttga tgagctttgg caagctttgg ggacagcttt ggggacagag cttgatgatt
     2041 tctgagattt ataagatatg caagcaaaaa ggacatgatt atgaaatcaa tcgaagatct
     2101 cgtttcaatt ctcaca
//
LOCUS       DAOMZL010000171         2109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_55488_length_2109_cov_12.233690, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000171 DAOMZL010000000
VERSION     DAOMZL010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55488_length_2109_cov_12.233690"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..358)
                     /gene="rpsO"
                     /locus_tag="PLQ30_08570"
     CDS             complement(89..358)
                     /gene="rpsO"
                     /locus_tag="PLQ30_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPW02538.1"
                     /translation="MALSKEDKAQLVLEYGKDSKNTGAIETQVAIISARIAYLTEHFK
                     THKKDTNSRRGLLKLVGQRRKLLKYLQRTDLESYRAIMEKLQIRK"
     gene            complement(349..1155)
                     /locus_tag="PLQ30_08575"
     CDS             complement(349..1155)
                     /locus_tag="PLQ30_08575"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HPW02539.1"
                     /translation="MKVLTWQEFQNNHSQEKLAATVGVFDGLHYGHQVLIDTIKLQSP
                     ALKPCIITFKENPKKILNPSSYKGSLLTLEQKLSGFQHMGIEYCVMIDFSDNFAKLSG
                     REFLAALYIANVQFIAVGVNFQFGHNLDTNAAKVEKLSEEIGLRSCIVENVMYRGHAI
                     SSSRIRHAVLEGRLGEAAEMIGRPYQVLARRETSVIDEKAEFFLPEDDLLIPPEGQYE
                     VFIKDNDTVQKTKVNVVHNYIALSLRMRSDNIRLAFVDKAVQQKENVVWL"
     gene            complement(1155..>2109)
                     /gene="truB"
                     /locus_tag="PLQ30_08580"
     CDS             complement(1155..>2109)
                     /gene="truB"
                     /locus_tag="PLQ30_08580"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPW02540.1"
                     /translation="EGSSEIMQHSGFFLVDKPKGFTSNRVLQDIRKSLSVQSSAYPQK
                     YGHAGTLDSFASGLLIILTGKITRLSRWFMHQNKEYEAVFRFGEETDTLDPQGKIMAI
                     GELPARETLERIIPSFCGNIEQIPPSYSAVHVGGERSYKIARAGNKPEISPRSIIIHD
                     IELESFQEKEATLRIRCSSGTYIRSLARDIGHACGSCATVISLRRTVIGPFSISEAHS
                     PQECNEKSIRVFSPDIAQSLGLATAVIKLDFVEHFIHGGTLPAKAVTLDCDQMTLFRN
                     PDFSFESKPVALFSEKGELLGIAEQKDKEYAPRVVMAEEPR"
BASE COUNT          553 a          500 c          397 g          659 t
ORIGIN      
        1 cattcaagtt tgtttatcat tacattcctt ctctagattt gcatatccga tttaatccgg
       61 agcaattaat attgctcgga ttggattatt attttcggat ctgcaatttt tccataattg
      121 cacgataaga ttcaagatcg gttctctgta ggtattttaa gagctttcga cgctgaccga
      181 caagcttaag aagccccctt cttgaattcg tgtctttctt atgagtctta aaatgctcgg
      241 tcagatatgc gatcctcgca gatataattg caacctgggt ctcaatcgca cctgtattct
      301 ttgaatcctt gccatactcg agcactaact gggccttgtc ctctttgctc aaagccatac
      361 tacattctcc ttctgttgaa cagccttatc aacgaacgcc aaccggatat tatccgatcg
      421 catgcgtaaa ctcaatgcta tataattgtg aacaacgttc actttggtct tctgcaccgt
      481 atcgttatcc tttatgaaca cctcatactg cccttccggt ggtataagca aatcatcttc
      541 gggcaaaaag aattctgctt tttcgtctat aacgcttgtc tcccgtctgg ctagtacttg
      601 atatggccta ccaatcattt ctgcggcttc tccaagcctt ccctcgagca cggcatggcg
      661 aattctgcta gagcttattg cgtggcctcg gtacatgaca ttctctacaa tacatgaacg
      721 caagccaatc tcttcactca gcttctcaac tttggcagca ttggtatcga ggttgtgccc
      781 aaactgaaaa ttcacaccca ccgctataaa ctgtacatta gcaatataca gtgcagcaag
      841 gaattccctt cccgagagtt tagcgaaatt atcagaaaag tcaatcatca cacaatattc
      901 tatacccata tgctgaaaac ctgataattt ctgctctaga gtcaagaggc tacctttata
      961 gctcgatgga ttgagtattt tcttgggatt ttccttaaaa gtgataatac acggcttcag
     1021 ggcggggctc tgtaacttta tagtatctat cagtacttgg tgcccataat ggagtccgtc
     1081 aaatacgccg actgtagctg caagcttttc ctgagagtga ttattttgaa attcctgcca
     1141 ggtcagaact ttcactatcg aggctcctct gccattacca ctctcggcgc gtattcttta
     1201 tccttttgct ctgctatacc tagaagctcc cctttttcgg aaaaaagcgc cactggtttt
     1261 gattcaaaag aaaagtctgg gtttctaaat aatgtcatct ggtcacaatc taatgttacg
     1321 gctttagctg gcaatgtccc tccatgaata aaatgctcaa caaaatccaa tttgattact
     1381 gctgttgcaa gacctaagct ctgcgcgata tccggtgaga agactctgat actcttctca
     1441 ttgcactctt gcggagaatg tgcttcgctt atggagaaag gtccaattac tgtccttctc
     1501 aacgatatca ccgttgcaca tgagccgcat gcatggccga tgtctcttgc aagagaacga
     1561 atataagtgc cagaagagca gcgaattcta agagtcgctt ctttctcttg gaaactctct
     1621 aactctatat catgtattat tatggatctc ggcgatattt cgggtttatt accggctctg
     1681 gctattttat atgatctttc cccgccgaca tgcacagcac tatatgatgg aggtatctgt
     1741 tcaatatttc cacaaaaaga aggaataatt ctttcgaggg tttctctcgc gggcaattca
     1801 cctatggcca tgatttttcc ttgaggatcg agagtatctg tctcttctcc gaatctgaaa
     1861 actgcttcat attccttgtt ctgatgcatg aaccatcgac taagacgtgt tatcttgcca
     1921 gtgagtatga tcaataaacc tgaagcaaaa gaatccagcg taccagcatg cccatacttc
     1981 tgaggatacg cagaggattg aaccgataag ctttttcgaa tgtcctgcaa tactctattc
     2041 gaagtgaagc ctttaggttt atccacgagg aaaaaaccgc tatgctgcat tatttcagaa
     2101 gatccttca
//
LOCUS       DAOMZL010000172         2088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_56385_length_2088_cov_11.001476, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000172 DAOMZL010000000
VERSION     DAOMZL010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56385_length_2088_cov_11.001476"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLQ30_08585"
     CDS             <1..396
                     /locus_tag="PLQ30_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPW02541.1"
                     /translation="TGAAKAVGEVLPEIKGKLTGMSFRIPTPAVSVVDLTFRSVKETS
                     IKEIDELLKKASRTYLKDILGYTDEELVSTDFIHDSRSSIYDSLATLQNNLPGEKRFF
                     KVISWYDNEWGYSNRVVDLLRYMASKDNF"
     gene            418..1677
                     /locus_tag="PLQ30_08590"
     CDS             418..1677
                     /locus_tag="PLQ30_08590"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPW02542.1"
                     /translation="MIKTVRDVSLTGKRIIMRVDFNVPMKNGVVQDDTRIVAALPTIR
                     YILDQKPKSLVLMSHLGDPEKDAAKAKEKAEKEGKPFDLEEYLSGKHRMKPVADYLSK
                     LLGVEVVFLPSCFGQKAAIDALPAGSVAMLENTRFHKEETAKDPALQEVLAKELASYG
                     DIYVNDAFGSAHRAHASTATVAKFVPIALGGLLMEKEVANLEPMLHNPPKPMVAIIGG
                     AKVSSKIGVLENLLKNASALIIGGGMAYTFLRAIGVAIGNSLVEEEYIETASELLKKA
                     AAQDVKVVLPVDHIAADHFAPDAKPIIVDSQALPDTLIGLDIGPKTLALYAETLKDAR
                     SVLWNGPVGVFEFDAFAKGTMEVAKLVAEATERGALTVVGGGDSVAAVNKFGLASKIS
                     HVSTGGGASLEYLEGKVLPGIACLEQK"
     gene            1821..>2088
                     /locus_tag="PLQ30_08595"
     CDS             1821..>2088
                     /locus_tag="PLQ30_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPW02543.1"
                     /translation="MRIYENITDLIGNTPMLYLNKLGKDLPCKIAAKLEFENPGHSIK
                     DRPALSMVLEAEKNGQLHSGMTIRFLTGIEIHPAARIGTGFFIDH"
BASE COUNT          578 a          428 c          536 g          546 t
ORIGIN      
        1 accggcgctg caaaggcagt gggggaagtg cttcctgaaa taaaaggaaa gcttacaggc
       61 atgtccttcc gcattcctac gccggcagtc tcggttgtcg atttgacatt tcgctcggta
      121 aaggagacct ccataaaaga gatcgatgag ctattgaaga aagcctccag aacctacttg
      181 aaggatattc ttggatatac cgatgaagag cttgtctcca ctgattttat ccatgatagt
      241 cgttcttcga tctacgacag cctggctacg cttcagaaca acttgcccgg agagaagcgg
      301 ttcttcaagg tgatttcttg gtatgataac gaatggggat attcgaatag ggtagttgac
      361 cttcttcgct atatggcgtc gaaggacaat ttctaggatc cgtctaagga gatcgcaatg
      421 atcaagacag tccgggatgt ttccttaaca ggcaagagga taataatgcg tgttgatttc
      481 aatgtgccca tgaagaatgg ggttgtacag gatgatacgc gtattgtagc cgcgctgcca
      541 actataaggt atatccttga tcaaaagcca aaaagccttg ttctgatgtc acatcttgga
      601 gatccggaaa aagatgcggc aaaggctaaa gaaaaagccg aaaaggaagg gaaacctttc
      661 gatctggaag aatacctatc cggcaaacac cgcatgaaac ctgtagcgga ttatctttca
      721 aaacttcttg gtgtcgaggt ggtctttttg ccatcttgtt ttggtcaaaa agccgcgatt
      781 gatgctcttc ctgcgggatc ggtggcgatg ctcgagaata cgcgcttcca taaagaagaa
      841 acggcaaagg acccggcatt gcaggaagtc ttggcaaaag agcttgcttc ctatggtgac
      901 atttatgtaa atgatgcttt tggatctgcc catagagccc atgccagcac tgcgacagtt
      961 gctaagtttg tgccgatagc gcttggtggt ttattaatgg agaaggaggt ggcaaatctt
     1021 gagcccatgc ttcataatcc tcctaaaccg atggtcgcaa ttatcggagg agcaaaagta
     1081 tcctcaaaga taggagtgct ggaaaatcta ctaaagaatg cttctgcttt gataattgga
     1141 ggaggtatgg cttatacctt tctcagagca ataggagtgg ctattggcaa cagtctggtg
     1201 gaagaagaat atatagaaac agcaagtgag cttctcaaga aagctgccgc gcaagatgta
     1261 aaagttgttc ttccagtaga tcatattgct gctgaccatt ttgcgcccga tgcaaagccc
     1321 ataatcgtag attcgcaagc tttgcctgat actctcatcg ggctcgacat cggtccaaag
     1381 acgctggctc tgtatgcgga aactctcaag gatgcaagga gtgtcctatg gaatggacca
     1441 gtgggggttt tcgaattcga tgcttttgca aagggcacca tggaggttgc caaacttgtt
     1501 gcagaagcaa cggagagagg ggctcttacg gttgtgggag gaggcgattc ggttgccgct
     1561 gtcaacaaat tcggacttgc ctccaagatt agccatgttt cgacaggtgg aggagcatct
     1621 ctggaatacc ttgaaggcaa agttcttcct ggtattgcgt gtcttgaaca aaaataggcc
     1681 tccctttcgg ttgagtaagc atatagcggc cgcccgaaag ggtggctgtt tttttatcta
     1741 atgtgatcga ttcggattcc gatccgcgcc gaccattatt catgattgaa cgatagattt
     1801 tatagaggag gagtccaaaa atgcgtattt atgagaatat cacagatcta attggcaata
     1861 ctccgatgct ttatctcaat aagcttggca aggatttgcc atgcaagatt gcagcaaagc
     1921 ttgaattcga gaatccaggc cattctataa aggataggcc tgcgctatcc atggtgctgg
     1981 aagctgagaa aaacgggcaa ttacattccg gaatgaccat aagatttctc acgggcattg
     2041 agattcaccc tgccgcaaga attggaacag gcttttttat cgatcatg
//
LOCUS       DAOMZL010000173         2087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_56429_length_2087_cov_17.933563, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000173 DAOMZL010000000
VERSION     DAOMZL010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56429_length_2087_cov_17.933563"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2035)
                     /locus_tag="PLQ30_08600"
     CDS             complement(<1..2035)
                     /locus_tag="PLQ30_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HPW02544.1"
                     /translation="MEVIDLAGNWNLVRSSDTTNRPIHIPGDIYSALMISGELPDPYQ
                     GENELMVQWPGKEDWIIERSFFISEAFLAASNIRLCADVLDTIAEVYINKVLVGTGKN
                     MFRPFEAEVRPALHPGENDIQVIIRSPVQAALRESQRLPYPIPASIYPVSSPHRNLIR
                     KAQCMSGWDWGPCLMTGGIYDGIRLVATDGLFIRSIQCHASALGDFGSRNDCGLDFSL
                     QISVEIEVPKPMKVVVSSYIDGKKATVEQECEQGNTVVSTNMVIQNPRLWWPHGYGEA
                     ALYDLVVDAAVQTTNPPQPHVIHKRIGFRSLQVVSQEDSIGRSFKFLINGKEIFAKGA
                     NWIPADALPSRWTRARIEHLLNSAIDANMNCLRVWGGGRYESDDFYELCDQKGILIWQ
                     DCMFSCALYPSSREFLSNVEAEITAQVKRLSDHPCIALWCGNNEALGAITWYEDSKKN
                     PARYIVDYDRLTEGVLGNVIRKLDPDRCFWPSSPSAGPDDFSDNWHSDERGDMHFWSV
                     WHEGKPFSAYLDVQPRFCSEFGFQALPCMSTIASFAHREEWNIGAPALEAHQKNPQGN
                     SIILESLLRYFRMPSGFHELLYLSQVQQAIAIRTAVEFWRSTMPRCMGTLYWQLNDVW
                     PAISWSSLNYDGSWKLLHYEARRFYAPILLALFIKDGIIHVRLVSESPNNHKAK"
BASE COUNT          546 a          516 c          492 g          533 t
ORIGIN      
        1 ctttggcttt atgattatta gggctttcac tcacgaggcg aacatgaatt attccatcct
       61 tgataaagag cgcaagcaaa ataggagcgt agaatctacg tgcttcgtag tgcaagagtt
      121 tccaagatcc atcatagttg aggctcgacc agcttattgc aggccataca tcgttcagtt
      181 gccagtatag tgttcccatg catcgaggca ttgtgctgcg ccagaattcc accgccgtgc
      241 gaatagcaat agcttgctgc acctgagaga gatagagcag ctcatgaaaa ccgctaggca
      301 tgcgaaagta tcgcagcaag ctttctagaa ttatgctgtt tccctgagga ttcttttgat
      361 gagcttcgag tgccggtgca ccgatgttcc attcttctct atgagcgaag ctagctattg
      421 tcgacataca tggcaaggct tgaaaaccaa attccgagca aaaccggggc tggacatcga
      481 gatatgccga gaatggtttg ccctcatgcc acacagacca gaaatgcata tctccacgct
      541 cgtctgagtg ccagttgtca gagaaatcgt cgggtccagc agaaggactt gaaggccaga
      601 aacaacggtc gggatccagc tttcgaatca cgtttccaag aacgccttct gtaagcctat
      661 cataatcgac tatatatcga gccggatttt tcttcgaatc ttcataccag gttatagcgc
      721 caagcgcctc attgtttccg caccagagag ctatacaggg atgatccgaa agtctcttta
      781 cctgtgcggt gatctcggct tccacattgg ataaaaactc ccggctggaa ggatagagtg
      841 cgcaggaaaa catacaatcc tgccagatca aaatgccttt ttgatcgcat agttcataaa
      901 aatcatcgct ctcgtagcgt ccacctcccc agacgcggag gcagttcata ttggcgtcta
      961 tggcggaatt tagcaaatgc tcgatgcgcg cccttgtcca tcgcgacggg agagcatctg
     1021 cgggaatcca gtttgcgccc ttggcaaata tttctttacc atttatcagg aacttgaagc
     1081 tcctgccaat actatcttct tgtgaaacaa cctggagcga tcgaaagccg attcgtttat
     1141 gtatgacatg aggttgaggg gggttggttg tttggacagc agcgtcaaca accagatcat
     1201 agagggcggc ctcaccatag ccatgtggcc accatagtct ggggttctga ataaccatgt
     1261 tggtgctgac taccgtattt ccttgttcgc actcctgttc cactgtcgct ttcttgccat
     1321 cgatatagga agagacaaca actttcatcg gcttgggcac ttcgatctct accgagattt
     1381 gaagcgaaaa gtccaggccg caatcattac ggcttccaaa gtctcccaga gccgaggcat
     1441 ggcattgaat ggagcgaata aatagcccat ccgttgccac aagtcgaata ccatcgtata
     1501 ttccgccagt catgagacag ggaccccaat cccatcccga catgcattga gccttgcgga
     1561 ttagattgcg atgcggagac gagacagggt agatcgaagc gggtattgga tatggtaggc
     1621 gctgtgattc gcgcaaagcc gcctggacgg gcgaacggat aatcacctgt atgtcatttt
     1681 cgccaggatg cagagccggg cggacttcgg cttcgaaagg ccgaaacata ttcttgccag
     1741 tccccaccag aactttattt atatatactt cagcgattgt atcgaggaca tccgcgcaga
     1801 gccttatatt ggacgctgcc agaaaagctt cggatatgaa aaaagatcgc tcgataatcc
     1861 agtcttcttt tccaggccat tgaaccataa gttcgttttc cccctggtaa ggatctggta
     1921 attctcccga aatcatgaga gccgagtaaa tatcgccagg tatatgtatt ggtcgatttg
     1981 tagtatcaga agaccttaca agattccaat tacccgcaag atcgatcact tccacttttt
     2041 gcctcccaaa agaattctcc tcacgccaaa ttctgaaaga tttttgt
//
LOCUS       DAOMZL010000174         2086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_56471_length_2086_cov_20.318562, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000174 DAOMZL010000000
VERSION     DAOMZL010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56471_length_2086_cov_20.318562"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2086)
                     /locus_tag="PLQ30_08605"
     CDS             complement(<1..>2086)
                     /locus_tag="PLQ30_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02545.1"
                     /translation="RLLPRILPPADQRIQNFIDQYLADLGLARIPQIPPNTLVLDHCG
                     MARELSLPPDEHKHASPTLTSYRVRNGVLHNPKSDRRTTEGVFHIAEGGLPIPLDKKA
                     VPKLCFAKLLEAALNPPLEIMELPFTAKENEKAHTFVSVLLRPIVRPEIPEYCEERSM
                     EIRFFAPGSLVANIDFVESIFGNSGDPFIPENDAAIDPVHWTGTTGCIILATHLTELT
                     KKEIGLPPWEQATERQRRDGMCWREPTEKYNDGKPFKIAARDESGIIITIIADNYFGY
                     SKKEIKSHISYSANLLGLAEEEHAGGALVFPSYNLGTRFVPDTNLKSKGHNLNDVYSI
                     MRSRIDMRQEGYGIDLTFPNIIYLPEDAYISLEDQMAHWVNDGIEESLRILPGNVYIN
                     PTGYQIRMEQHSTSGAWRLIGTSAEGLLCHKPCTVSGGGKSEIAKSIQDAIVYMPIVI
                     ADFDKDMQEARKIIEKDYVNRFRDQSENLGKDTRPILSPKRSLGSVIKLLSPSPAYTD
                     EYNEWLRSIPERIKSLVFLVKRFYRPDWGLDWMSHFSVDAVNGTTGNILKFEGKPIMG
                     SYLRVGKNEKGLNRFFKLRQDFMPAFKLQWEDDITASIIVPVNQLENLPDWASKHLSL
                     KFAKNCEARFFQRPDDAIIRGYDKQTEKDLARYDNFLSNFEPLTRADASRIIENTIHF
                     SEYTEPMRKFIE"
BASE COUNT          490 a          448 c          562 g          586 t
ORIGIN      
        1 tcttctatga attttcgcat aggttcggtg tattcggaga aatgtattgt gttttcgata
       61 atgcgagagg cgtcggcgcg ggtgagaggt tcaaaattcg acaggaaatt gtcgtatcgc
      121 gctagatctt tttcggtctg tttgtcatag cctcgaataa tggcatcgtc aggccgttga
      181 aagaatcgcg cttcgcaatt cttggcaaat ttcaggctca gatgtttgga ggcccaatca
      241 ggcaaatttt ccagttggtt gacaggcacg attatcgaag cagtaatatc atcttcccat
      301 tgaagtttga aggctggcat gaagtcctgc ctaagcttga agaagcggtt taggcctttt
      361 tcattttttc ctacgcggag ataggatccc attatgggtt tgccttcgaa tttcaagata
      421 tttcctgtag taccattgac cgcatccacc gagaaatgcg acatccagtc gagaccccaa
      481 tcgggcctgt agaatcgctt tactaaaaac acgaggctct ttatgcgttc cggaatggaa
      541 cgcaaccatt cattgtattc gtcggtatat gcaggcgaag gggagagtag ctttatgacc
      601 gaaccgaggg agcgctttgg agagagtatc ggccttgtgt ctttcccaag attctccgat
      661 tgatcgcgga aacgattgac atagtctttt tcgattattt ttctggcttc ctgcatatcc
      721 ttatcgaaat ccgcaatgac tataggcatg tacacgatag catcctggat cgattttgct
      781 atctcagact tgcctccgcc cgataccgtg cagggcttat gacagagaag tccttctgcg
      841 gaagtgccaa taagtcgcca tgcaccggat gttgaatgtt gctccatgcg aatctgatag
      901 cccgttgggt ttatgtagac attgcccgga agaatacgca gagattcttc aataccatcg
      961 ttgacccaat gtgccatctg gtcttccaga gaaatatagg catcctcggg tagatagatg
     1021 atatttggaa aggtaagatc gatgccatag ccttcttgtc tcatatcgat gcggctgcgc
     1081 attatggaat aaacgtcatt gagattgtgg cctttggatt ttagattggt gtcgggcacg
     1141 aagcgcgtgc caaggttata ggaaggaaag acaagagctc ctcccgcatg ttcttcttcg
     1201 gcgaggccta gaaggttagc agaataagag atatgagact ttatttcttt tttggagtag
     1261 ccgaaatagt tgtccgcaat tatcgtaatt atgatcccgg attcatcgcg agcagctatt
     1321 ttgaagggtt tgccatcatt gtacttttcg gttggttcgc gccagcacat tccgtcacgg
     1381 cgttggcgtt cggtagcttg ctcccatggg ggtagtccaa tctctttctt tgtaagctcg
     1441 gtgaggtgcg tagctaggat tatgcagcct gttgtgcctg tccagtgaac tggatctatg
     1501 gccgcatcgt tttcggggat aaatggatct ccagaatttc caaatatgct ctcaacaaag
     1561 tcgatattgg cgactagcga accaggggca aaaaagcgga tctccatcga tctctcttcg
     1621 cagtattcgg gaatttcagg acgaacgata ggccgcaaca atactgatac gaaagtatgt
     1681 gccttttcgt tctcttttgc agtaaagggc agttccataa tctcgagtgg agggttcaga
     1741 gcggcctcta agagcttagc aaagcagagc ttaggcactg cctttttgtc gagtggaatt
     1801 gggagaccgc cttccgctat atggaagact ccctcggtgg tccgtcgatc gctcttgggg
     1861 ttgtggagga cgccgttgcg gacacggtac gaggtgaggg taggggacgc atgtttgtgc
     1921 tcatcgggcg ggagagagag ctcgcgagcc ataccacagt gatcgagaac cagtgtgttt
     1981 gggggtatct gcggaattcg ggcaaggccc aggtctgcca ggtattgatc gatgaagttt
     2041 tggatgcgct gatcagctgg tggaaggatt ctaggcaaca gcctgt
//
LOCUS       DAOMZL010000175         2062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_57511_length_2062_cov_21.415047, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000175 DAOMZL010000000
VERSION     DAOMZL010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57511_length_2062_cov_21.415047"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..1577
                     /locus_tag="PLQ30_08610"
     CDS             240..1577
                     /locus_tag="PLQ30_08610"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HPW02546.1"
                     /translation="MKGSPYERGYQHGILLSKQINETLTKGLTGAAAVVAYNQGCSFE
                     EGFERLRQGKREAEGFVPPEFREEMAGIADALAKTGSQLDLHDVLLWNTMYDSWCFFA
                     HPNSSNQAQTFERQKYPIGCSSFSAWGEATRDGTFIFGKNMDNLNLPGILEGRMLVVC
                     DPDNGLGHVNVSHPGMIAIDGGINENGIEMMTQYSPSIYETMRGCGIAVLSRLILQNA
                     SRVDDALNILTVYPRCTGINYHVGDAKAQRAIVIETNAKQIAFRRPYKQDILWTTNHY
                     NCYPGWMGYQGENLVEGQKLAFCLKDVSSIESWQESLKDKSNPYLSATGRFRQYEKLL
                     SELYGEITMESGIEILSDRHNPDTGELRDWETAPKARNDGNTIAFWAAPTTFAEKVKF
                     YKSNLETSIVAHTGNLWSMIATPLNGDFRIAMSDFPAQRGPYVHFNLFEELNR"
     gene            complement(1683..>2062)
                     /locus_tag="PLQ30_08615"
     CDS             complement(1683..>2062)
                     /locus_tag="PLQ30_08615"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW02547.1"
                     /translation="STGGADLNGVQVYNYLNLEVSNPRWDKFKAAFAKDHNGQMPFSL
                     SPNYYDALYMIKEAIEKTGVTGDPKKLKEERKKIADYCANVKGFKGLLYEWDMKDGVP
                     TNKPSYIFEIQNGVKQFVLEVRP"
BASE COUNT          643 a          423 c          458 g          538 t
ORIGIN      
        1 tagcaaaagc cattagatat gcagattctg catatggata taagagctat tctgaaatag
       61 taaaaaagca ataaatctgc taatattcta agcgaactaa gcaggcaaga aagtcggctc
      121 tagtcgctta gctctattta tgactatcgg agggttcaat cattaagcaa gaaaataatg
      181 taatcgatag atatggaaaa ggatatctcg agctgaaaaa tggtattctg gttctgcaca
      241 taaaaggatc accttatgag cgtggatatc aacatggtat ccttctctca aaacaaataa
      301 atgaaacatt aacaaaaggc ctaactggtg cagctgctgt cgtagcttac aaccagggtt
      361 gtagctttga agaaggattc gagagactta gacagggcaa gcgagaagct gaaggatttg
      421 ttcctcctga gtttagagaa gaaatggcag gtatagcaga tgctttagca aagaccggat
      481 cgcaactcga tctacatgat gttcttttgt ggaatactat gtatgattct tggtgctttt
      541 tcgcacaccc aaatagcagc aaccaagctc aaaccttcga gaggcaaaaa tatcctattg
      601 gatgctcaag tttctctgct tggggagaag ctactcgaga cggaacattc atattcggga
      661 aaaacatgga caacctaaat ctgccgggca tacttgaagg aagaatgttg gtggtatgcg
      721 atcctgacaa tggattaggg catgtaaatg tctcacaccc tggaatgatt gcgatagatg
      781 gaggcataaa cgagaacggc attgaaatga tgacccaata cagcccctcc atctatgaaa
      841 caatgcgagg atgtggcatt gctgtgctct cacgcttgat attgcagaat gcaagcagag
      901 tagacgatgc tctgaatatc ctcacagtat atccaagatg tacaggaatt aactaccatg
      961 taggagatgc aaaggctcaa agagccattg tgatcgaaac aaacgcaaag caaatagcct
     1021 ttagaagacc ttataagcaa gatatcttat ggacaacaaa tcattacaac tgctatccag
     1081 gttggatggg atatcaagga gaaaatctcg ttgaaggaca aaagcttgca ttctgcctca
     1141 aggacgtaag cagtatcgaa tcatggcaag agagcctcaa agacaaatca aatccttacc
     1201 tttccgcaac gggaaggttt aggcaatacg aaaaattgct tagcgaactc tatggggaaa
     1261 tcaccatgga aagcggtata gaaatactga gcgatcgcca taatccagat accggcgaac
     1321 tacgagattg ggagacagct cccaaagcgc gaaatgatgg caatactatt gctttttggg
     1381 ctgcccctac cacctttgcc gaaaaggtca agttctataa aagcaatctt gaaacatcta
     1441 ttgtagccca tacggggaat ttatggagca tgattgccac tccgctgaat ggtgacttta
     1501 gaattgcaat gagcgatttc cctgcccaga ggggaccata tgtgcatttt aatctcttcg
     1561 aggaattgaa taggtgataa aagcggagag attaagaaaa aaccataatc ttacgagagg
     1621 gggcgccgca tgcgcggcgc cccctctctg attgattcac actctcttat aaactacttt
     1681 tcttatggcc tcacctcaag tacgaattgc tttaccccat tctgaatttc gaatatataa
     1741 gaaggcttat tggtaggcac tccatccttc atgtcccatt catagagtag acccttaaag
     1801 ccttttacat ttgcacagta atcggcaatc ttcttgcgct cttctttgag cttctttgga
     1861 tctcctgtga ctcctgtctt ctcgatcgct tccttgatca tatagagtgc gtcatagtaa
     1921 ttcggcgata gcgagaaggg catctggcca ttgtgatctt tcgcaaaagc tgctttgaat
     1981 ttgtcccagc gaggattgga tacttctaag ttgaggtagt tatagacctg aacaccattc
     2041 aaatctgctc cgcctgtaga gt
//
LOCUS       DAOMZL010000176         1941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_63495_length_1941_cov_19.448568, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000176 DAOMZL010000000
VERSION     DAOMZL010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63495_length_1941_cov_19.448568"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PLQ30_08620"
     CDS             <1..762
                     /locus_tag="PLQ30_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPW02548.1"
                     /translation="KVASICIVGNDYDALTPVLKKASAQGIKVFSLDSSVNPQARLTH
                     VNQADSEKIGQTLIKAAYDMAGGKGEIAILSATSQASNQNIWIDFMKKELAKPEYKDL
                     KLVKIAYGDDLRDKSVSETEGLLRSYPNLKVIIAPTTVGIAAAGKVLTDKGLKGKVFL
                     TGLGLPSEMAEYINNGVCPYMFLWNPIDVGYLGSYVATALVSGKITGKVGEKFSAGRL
                     GNYTITQAPDGGTEVLLGPPFKFDKSNIEEWKKVY"
     gene            843..1157
                     /gene="rhaM"
                     /locus_tag="PLQ30_08625"
     CDS             843..1157
                     /gene="rhaM"
                     /locus_tag="PLQ30_08625"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HPW02549.1"
                     /translation="MKRYAFIMQLKPGNEAEYKRRHDEIWPELKDELRKAGISNYSIY
                     LDPETLRLFAIQNLAEDETSDSLSRLPIMRKWWDSMKDLMETNADNSPKVWTLKEMFH
                     LD"
     gene            1176..>1941
                     /locus_tag="PLQ30_08630"
     CDS             1176..>1941
                     /locus_tag="PLQ30_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPW02550.1"
                     /translation="MNLDSIIRLSRRYGTDSRYVLAGGGNTSLKQDDVMAVKASGISL
                     GTIDEKGFVLLSLPRLIDIFEAVLPSSAAERDKIVLERLLASRLPGQTMRPSVETLLH
                     ALFPFKFVVHLHPALVNGLLCSAEALEKTPELFGEEAEVLPYTTPGYTLADRVRTLFR
                     THKSMGKSPIRILFLQNHGVFVAADNADEIDDLYRYIFDTLGKCVKPLPVDDDSYSIV
                     DKAKNKTLPEKMKIAMEAMSSEFNVRFFFTSNSLLDY"
BASE COUNT          557 a          425 c          471 g          488 t
ORIGIN      
        1 aaagtcgcct cgatctgcat tgtcggcaat gactatgacg ccctgacgcc tgtcctaaag
       61 aaagcaagtg cgcaaggcat caaggtgttt tctctcgatt cttcggtaaa tccacaggcg
      121 cgactgaccc atgtcaatca agcggattca gagaagattg gccagacgct cattaaggct
      181 gcgtatgaca tggctggcgg caagggcgaa atagctattc tctcggcgac aagccaagca
      241 tcgaatcaga acatctggat cgacttcatg aaaaaagagc tcgccaagcc agaatataaa
      301 gacctaaagc ttgtcaagat cgcctatggc gatgatttac gcgataaatc ggtctccgaa
      361 accgaaggac ttctacgatc ctatccaaac ttaaaggtga tcattgcccc gaccacagtt
      421 ggaatcgcgg cagctggaaa agtactcacc gacaaagggc tcaagggcaa agtcttcctg
      481 accggtcttg ggttgccttc cgagatggcc gaatacatca ataatggagt ctgtccttat
      541 atgttccttt ggaatccaat cgacgtgggc taccttggat cttatgtagc gaccgccctt
      601 gtgagtggca agataacggg caaggttggg gagaagttta gcgctggacg cctaggaaac
      661 tacaccatca ctcaagcgcc cgatggcggt accgaagtat tgcttgggcc cccattcaaa
      721 ttcgacaaga gcaacatcga agaatggaaa aaagtgtatt gagcgattta tgcgggcttg
      781 ctgaaatatg caagcccgca atatccattt ccattaggcc ccattgatta aaggatgaaa
      841 aaatgaaacg ctatgcattt attatgcagc tcaaacctgg aaatgaagcg gaatacaagc
      901 gccgacatga tgaaatatgg cccgaattaa aggatgagct gcggaaggca gggatttcga
      961 attattcgat ttacttagat cctgagacat taaggctttt tgcaattcaa aacctagctg
     1021 aagatgagac aagcgattcc ttgtccagat taccaataat gcgcaagtgg tgggattcga
     1081 tgaaagacct gatggagacg aatgctgata attcgcccaa agtctggacg ctcaaagaaa
     1141 tgttccatct ggactgaatg catgaaggtg tgtgcatgaa tctcgattct attataaggc
     1201 tctctcgccg ctacggcacg gactcccgat acgttttagc cggaggagga aacacttctc
     1261 taaaacaaga tgatgtcatg gcggtaaagg cttcgggaat ttcattggga acaatcgatg
     1321 agaaaggctt tgtattgcta tcgcttccta ggctcatcga tattttcgag gctgtgcttc
     1381 cttccagcgc ggctgaacgt gacaaaatcg tgctcgaaag gcttcttgcc tcccggctgc
     1441 cggggcagac aatgcggcca agcgttgaaa ccttactgca tgccttattc ccttttaagt
     1501 tcgttgtgca tctgcatccg gcgctggtaa atggtctact atgttcagcc gaagctctcg
     1561 aaaagacacc tgagctattt ggagaagaag ctgaagtttt accctatacc acgcctggat
     1621 atactcttgc ggatagagtc cgaacattgt ttagaacaca caaatccatg ggcaaaagcc
     1681 caattagaat acttttcttg cagaatcatg gagtattcgt cgctgcagat aatgctgatg
     1741 agatagatga tctatatagg tacatttttg atactctagg aaaatgtgtg aagccgttgc
     1801 cggtggatga tgattcttat tctatagtag acaaggctaa gaataaaact ctgccagaga
     1861 agatgaaaat agcgatggaa gcgatgtcct cggaatttaa cgtaagattt ttctttacgt
     1921 caaattcgct tttggattat t
//
LOCUS       DAOMZL010000177         1913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_65022_length_1913_cov_15.035522, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000177 DAOMZL010000000
VERSION     DAOMZL010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65022_length_1913_cov_15.035522"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..1169
                     /locus_tag="PLQ30_08635"
     CDS             27..1169
                     /locus_tag="PLQ30_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW02551.1"
                     /translation="MSAKKKFSTFLSRYAVVIIFLVVTIAATPPSGLSIRYILQELIT
                     RLGRNSFLVLALLLPIYAGMGLNFGMTLGAMAGQIGLIFAVNYSIAGWQGLVFALLVG
                     LPISVLLGWICGLVMNRAKGREMVTGYILAFFMNGIYQFFVMYMLGSVVPMGNKAIVL
                     SRGYGIRNTLNLESVRQSLDNILVLRFAGFTFPVVTFIVIAVLCVFIIWFKKTKLGQD
                     MRAVGQDMAVAESAGIPVERTRIIAIIISTVLACAGQIIFLQNMGNLATYNAHDQTGF
                     FAVASILVGGASVTSANIANVFIGVMLLHAMFVVSPLAGQNLFGSAMIGEYFRQFIGY
                     GVIALSLVLYAWRSKKAAAEARISLRGANNNHKDKIEVTTKNGGQA"
     gene            1166..1693
                     /locus_tag="PLQ30_08640"
     CDS             1166..1693
                     /locus_tag="PLQ30_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02552.1"
                     /translation="MNEPTGEKSIGNAKNTKKRRLIIRSGLLVVYVLLIAFMIHSGRR
                     HTILIDNKDAHDDSYIGIDGMEVSIDNKEPLEYYRGDRDKAVVQGQKHTIKVEIFDDG
                     RKIEESFTVPLWTDMVIIYVPRLVNGIEPWLEPFTIAEQIEESQESWSPDDEMSFESM
                     GSPQLEGVENTTPTP"
BASE COUNT          503 a          395 c          475 g          540 t
ORIGIN      
        1 ctcacgcgca agatgaaggt gtcaaaatga gcgcaaagaa aaaattctcg acatttctat
       61 cacgatatgc tgtagtcatt atttttcttg ttgttacgat agcggcaact ccgccatcag
      121 ggctatctat taggtatata ttacaggagc tcattacaag gctagggaga aattccttcc
      181 tcgtattggc tctactattg ccgatatacg ccggtatggg gctcaatttc ggcatgaccc
      241 tgggtgctat ggcaggccag atagggctta tatttgcagt gaactacagc atagctggat
      301 ggcaggggct tgtgtttgct cttcttgtgg gcttgcctat ctcggtgctg cttggctgga
      361 tctgcggtct tgtcatgaac agagcaaaag gacgcgagat ggtaacaggc tacatccttg
      421 cgttttttat gaacgggatc tatcagttct ttgttatgta catgctgggc tcggtcgtac
      481 cgatggggaa taaggctatt gtcctgtcgc gcggttatgg cattcgcaat accctaaatc
      541 ttgaatcagt gcgtcaatcg ctggacaata tccttgtatt gcgcttcgca ggatttactt
      601 tcccagtggt gactttcatt gtaatagccg tactttgtgt ttttataatt tggtttaaga
      661 agactaagct gggacaagac atgcgagcgg tgggtcagga tatggcagtg gccgaatcag
      721 ctggcatccc tgttgaaagg acgcgtatta tagcgattat catctcgacg gtgcttgctt
      781 gtgccggaca aattatcttc cttcaaaata tggggaacct tgctacctat aatgcacacg
      841 accagacagg tttctttgcc gttgcttcta tattggttgg cggtgcatcg gttactagcg
      901 caaatatcgc taacgttttc ataggtgtta tgctcttaca tgctatgttt gttgtatcgc
      961 cacttgctgg gcagaatctt tttggctcgg ccatgattgg agaatatttc cgccaattca
     1021 tcggttatgg tgtgatagcg ctttcgctcg tgctctatgc ttggagatct aagaaggctg
     1081 ccgcagaggc tcgaatcagt ctacgtggag cgaacaataa tcacaaagac aagatcgagg
     1141 tcacgacgaa aaatggaggg caggcatgaa tgaaccaacg ggcgagaaaa gtatcggtaa
     1201 tgcaaagaat acaaagaaac gtcgcctcat aattcgttcc ggccttctcg tggtttatgt
     1261 gcttctcatc gcttttatga tccactctgg caggcgacac accatactca tcgacaacaa
     1321 ggatgcgcat gatgactcct acattggcat agatggaatg gaggtgtcga tcgataataa
     1381 agaacccctg gaatattatc gcggagatcg cgataaagca gtggttcagg ggcaaaagca
     1441 cacaatcaag gttgagatat tcgacgacgg gcgcaaaatc gaagagtctt ttactgtccc
     1501 tctttggacc gatatggtaa taatttatgt acctaggctg gttaatggaa ttgagccatg
     1561 gctagagcca tttaccatag ctgaacaaat tgaggaatct caagaatcgt ggtcgcccga
     1621 tgatgaaatg agctttgaaa gcatgggtag cccacagcta gaaggcgtag aaaacactac
     1681 tccgactcca taaaaaattc caaaaacgag ctctcaataa ttcatggctc atagagtctt
     1741 gaattttcgc tccaaatgcg aagaggcttg atttgtattt tcaaggtgta gataaattct
     1801 gcatcttcaa tatcaaataa ggaggacgta tgagaaagct tgtagcagtt attctagtac
     1861 tagcttttct agtggtgggt gccgatgtat tcgcccaggc caaggccaaa ttc
//
LOCUS       DAOMZL010000178         1899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_65782_length_1899_cov_10.645336, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000178 DAOMZL010000000
VERSION     DAOMZL010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65782_length_1899_cov_10.645336"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1717)
                     /locus_tag="PLQ30_08645"
     CDS             complement(119..1717)
                     /locus_tag="PLQ30_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPW02553.1"
                     /translation="MVKEALDSEKFHYVYRKDLGYYTVNIDEPYWDRQKKQMRHRYKM
                     VGKSSIKGGPIEFGPSYRAIRAQRGYIDALSASTTIYEGELLLLQHIAQELGLGRILT
                     KVLGKEDAKKVLALASYVVCTHEPLSYAAPWLEARGLGHLGLEAPRISELLPTFTADR
                     QNSFFSLWLKEKASSEALCYDITSVSSYARDNDLIEYGYNRDHEHLRQINLALVSNKH
                     TSLPLWYAPLPGSLNDTKTLKKLVLEMKKLDVGSFSLIMDRGFYSKENLEFLVRAAIK
                     FMIPIPSTVEWQKRLILENRATMFTNVEGYIPTEEGTMLQSLTVYKPFEDGERAWLHI
                     YYDSTIRSHAEQEFMAYYKQCFDEFSSGELDPTHQDFYNEYFRKGYKTKNGQKVIPKK
                     DPVEVFREDTAGYWCLYTTSEKDAKAALEVYRERSEIEQLFDDLKNSLDCNRLRVHTK
                     AGMQGRLFIQFIALILLSELKRHIREQHELLAKYGNHKAILMRVASFSSIRFQGKYKE
                     LYSAPTKAQEIIFDAFGVEYPKNE"
BASE COUNT          478 a          463 c          406 g          552 t
ORIGIN      
        1 ctcgaagaaa aattcaatct cttgacatgt tgtctaacat cataacacct gattgccttt
       61 agtatcgtat tgatagccgt atatacttgc ccaaaatttc gcacagattt aagcatgttc
      121 attcattttt ggggtattcc accccaaacg cgtcgaagat tatctcctgc gccttggttg
      181 gtgcagaata gagctcttta tacttgcctt ggaatctaat ggaagagaat gaggcgaccc
      241 tcataaggat tgccttgtgg ttcccatact ttgcgagtag ctcatgctgc tcacggatat
      301 gccgtttaag ctcacttaag agaatgagcg caatgaactg gatgaaaagc cttccctgca
      361 tccccgcttt ggtatgtacc cggagccggt tacagtcaag actgttcttt aagtcatcga
      421 agagctgctc aatctcactg cgctcacgat agacttctaa tgcagccttc gcatcctttt
      481 cactggtggt atataggcac caataacccg ctgtgtcctc cctgaatact tcaaccgggt
      541 ctttctttgg aatgaccttc tggccgttct ttgtcttata gcccttccta aaatattcgt
      601 tataaaaatc ctggtgggtt gggtcgagct ctccagaaga aaactcatcg aaacactgtt
      661 tataataggc cataaactct tgctccgcat gagagcggat agtgctatca tagtaaatat
      721 gaagccatgc cctttcgcca tcctcaaatg gcttatagac cgtgagcgac tggagcatgg
      781 tgccttcctc agtcggaata tacccctcaa cattggtgaa cattgttgcc cggttctcga
      841 gaataagcct cttttgccat tcaacggtac ttggaatggg aatcatgaat ttgatcgctg
      901 ctcttacaag aaactcaagg ttctccttgc tataaaagcc tctgtccata ataaggctaa
      961 agctccccac atcgagcttc ttcatctcca agaccagctt cttgagggtc tttgtgtcat
     1021 tgagggaacc aggtaagggt gcataccaga ggggtaagct ggtatgctta ttgctgacaa
     1081 gggcaagatt gatctgtcgt aagtgctcat ggtcacgatt gtacccatac tcaatgagat
     1141 cgttatccct agcataggaa ctgaccgagg taatatcgta gcagagtgct tcgctcgacg
     1201 ctttttcttt cagccataat gagaaaaagc tgttctgtct atcagccgtg aacgtcggca
     1261 aaagctcact gatccgggga gcttcaagcc caagatgccc taatcctcgt gcctcgagcc
     1321 agggcgctgc atatgagagt ggttcatgcg tacacaccac ataggaggca agcgcaagca
     1381 ccttctttgc atcttccttc ccgagcactt ttgtcaatat tcgtcctagg ccaagctcct
     1441 gcgcgatatg ctgtaggagt aacagctccc cttcatagat agtcgtgctt gccgagagcg
     1501 catcgatata cccgcgttgt gcacgaattg cacgatagct cggtccgaat tcgatcggtc
     1561 ctcccttgat actgctcttt ccgaccattt tgtaccgatg gcgcatctgc ttcttttgtc
     1621 tgtcccaata gggttcatcg atattgacag tatagtagcc gaggtccttt cggtagacat
     1681 agtggaactt ttcactatcc agagcttcct ttaccatgct tcatattgta gcatgtattg
     1741 aattattgcg caacaaccac aacaccataa tttatcagaa attaacactt ttgagtcttc
     1801 ttaatattct cccatgctta agattgtgcg aaatttttag gtaataaaag aataggagcg
     1861 ctagaatggc gctcctatct tatttggaac ctggaagat
//
LOCUS       DAOMZL010000179         1883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_66765_length_1883_cov_18.363786, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000179 DAOMZL010000000
VERSION     DAOMZL010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66765_length_1883_cov_18.363786"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..417
                     /locus_tag="PLQ30_08650"
     CDS             1..417
                     /locus_tag="PLQ30_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPW02554.1"
                     /translation="MTKEIQMSWLEDMAFESLLDGHRLVLDADDNSGGKDRGPRPKGL
                     ILAALGACTGMDVISILKKMREPITWFNMKLSGEVAEEHPKKFTSFKIIYQFKKSDGL
                     KPENVHKAVELSQTKYCGVSATLKDGHEVAWEIEYI"
     gene            647..>1883
                     /locus_tag="PLQ30_08655"
     CDS             647..>1883
                     /locus_tag="PLQ30_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPW02555.1"
                     /translation="MAFDVHKMRNIGISAHIDSGKTTLSERILFYCRKIHAIHEIKGK
                     DGSGAAMDSMELERERGITIQSGATNVQWKNHSINLIDTPGHVDFTIEVERSLRVLDG
                     AILVLCSVAGVQSQSITVDRQLKRYHVPRLAFVNKCDRMGANPLKVKAQLAEKLGLSP
                     AFITIPIGLEDKFEGVIDLIDMRAIYFDGPNGEDLRYAEIPMHLLDDAERYREELLEA
                     ASMFSDELAEAYLDGNPTKELLIKAIRKGTIQEKLVPVFVGSAYRNKGVQPLLDGVIN
                     FLPDPSERQNKALDLSNAEAEVELQANDQEKTVALAFKLEEGQYGQLTYVRIYQGMIR
                     KGDELYNTRARKRFRVGRLVRMHSSSMEDIQEGNCGDIVALFGIECASGDTFCHPDLN
                     YTMTSMYVPEPVISLAITPK"
BASE COUNT          541 a          401 c          451 g          490 t
ORIGIN      
        1 atgaccaaag aaatccagat gagttggctc gaagatatgg cctttgaaag cttgttggat
       61 gggcatcgct tagttttgga tgcagacgat aatagtggcg gaaaagatcg cggtccccgt
      121 ccaaaaggac tcattcttgc cgcgcttgga gcatgcactg gcatggatgt catctcgatt
      181 ctaaaaaaga tgcgcgagcc cataacctgg ttcaatatga aattgagtgg agaagttgct
      241 gaagaacacc ctaagaaatt cacaagcttc aagattatct atcaatttaa aaaaagcgat
      301 ggactcaagc ctgaaaatgt tcacaaggcc gtcgagctct cgcagaccaa gtattgcgga
      361 gtcagtgcga ctttgaaaga tggccacgaa gtcgcttggg aaatcgaata catataggcc
      421 tctgcaccgc cttaggatta acgtgatgtg cgctgattga gatttgaatt ggcgctgaga
      481 cttgttcttt ttcatcatct tttctataat tgcgaaacgg tagctcattt tctagtatct
      541 gttatgcttt gcattatatt cagcaaatca gtcttgctgc caccactttg acacttcttt
      601 tgtctggtgt attcttaatt tcgtgattta agaggtatag aaaaagatgg cattcgatgt
      661 ccataaaatg agaaatatcg gcatctcagc gcatatagat tcaggaaaaa cgactttatc
      721 agagcgtatt ttgttttatt gccgaaaaat acatgcgata cacgaaatca agggcaagga
      781 cggctccggc gctgcaatgg attcgatgga attggaacgc gagcgaggca tcacaattca
      841 atctggagca accaatgtac aatggaaaaa ccatagcatt aacctcatcg atactccagg
      901 acatgtcgac ttcacaatcg aggtggaaag atctctccgc gtattggatg gagccatcct
      961 cgtgctctgt tcagtagcag gtgtgcagtc tcaatctatc actgtcgatc ggcaacttaa
     1021 gcgataccat gttccccgtc tggcttttgt caataaatgc gatcgcatgg gtgcaaatcc
     1081 cttaaaggtc aaggcgcagc tcgcagaaaa actgggctta agccccgcgt ttataacaat
     1141 tcctatcggg ctggaagata aattcgaagg tgtcatagat ttgatagaca tgagggctat
     1201 atatttcgat ggtcctaatg gcgaagatct gcgttatgcg gaaatcccga tgcatctcct
     1261 cgatgatgcc gagagatacc gagaagagct tctggaagca gcttcgatgt tctcagatga
     1321 gcttgcggaa gcgtatctag atggtaatcc aaccaaagaa ctgttgatca aggctatcag
     1381 gaaaggaacg atccaagaga aattagtacc tgtatttgtt ggttcagcat ataggaataa
     1441 aggtgtacaa ccattgctgg acggggtgat aaattttttg cccgatcctt cggaaagaca
     1501 aaacaaagct ctcgatctct ccaatgcaga ggcagaagtc gagctgcagg cgaacgacca
     1561 agaaaaaact gtcgcgcttg ccttcaaact cgaagagggc caatatggcc agttaaccta
     1621 tgtgcgcata tatcaaggca tgatccgtaa gggcgatgaa ctctacaaca cgcgcgctcg
     1681 caagcgattt cgtgtaggaa ggcttgttcg aatgcattct tcttcgatgg aagacataca
     1741 ggaaggcaac tgcggagata ttgtggctct tttcggtatc gaatgtgcat ccggcgacac
     1801 tttttgtcat cctgatctta actataccat gactagcatg tatgtgcccg agccggtcat
     1861 atcgctcgcc ataacgccta aag
//
LOCUS       DAOMZL010000180         1842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_69215_length_1842_cov_13.773363, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000180 DAOMZL010000000
VERSION     DAOMZL010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69215_length_1842_cov_13.773363"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PLQ30_08660"
     CDS             <1..708
                     /locus_tag="PLQ30_08660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW02556.1"
                     /translation="DTTETKLRKELEQVNQELERFTYSVSHDLRAPLRSIEGFSRALK
                     EDYEDRLDREGQDYLQRIRAACQRMAAMIDAILQLSRIGRYEMHKEKVNLSAIAQDIA
                     SDLETTSPERKVEFSIAEGVFAAGDPMLLRMVLENLIGNAWKFTQEHELGRIEFGMRQ
                     ESSKHVYFVRDNGLGFDMKYAAKIFDPFQRLENAKALSGSGIGLASVKRIIHRHGGEI
                     WAESAVGKGSTFYFTLE"
     gene            741..>1842
                     /locus_tag="PLQ30_08665"
     CDS             741..>1842
                     /locus_tag="PLQ30_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPW02557.1"
                     /translation="MNKSLRVLLIEDSEDDTLLILRELRKGGYFPVHERAETESAMRS
                     ALSSQKWDIILSDYNLPEFCADEALSIFRESGIEAPFIIVSGTIGEEKAVQLMKGGAN
                     DYIMKNNLRRLVPAIERELKESEDRKSRRLAEDSYRKSDFVVNASRDIMALINKSGIC
                     ETVNSSLCKIFGKEEQKEIVGHPVSDVWGNEVYEQKIKPYLERCQNGEEINTEDWFDI
                     EGMGSQCFEINYIPYRDNDGVVTHTIMAAHNITNRKTAEQDLDTSYRNLQKTLEGIVK
                     ALSALVEMRDPYTAGHQNRVARIARAIAEELDLSEDAVQGIWVASLIHDIGKIRVPAD
                     ILSRPGHLSSVEFELIKEHPRTGYEILREIDFP"
BASE COUNT          554 a          359 c          466 g          463 t
ORIGIN      
        1 gatacaacag aaacgaagct tcgaaaagag ctggagcaag taaaccagga actcgagaga
       61 ttcacatatt ctgtctccca tgatctgcgt gctccgctta ggagcataga gggtttcagt
      121 cgggctctga aagaagatta tgaagatcgt ctagatcgag aaggacagga ttatcttcag
      181 cgcataagag cggcatgtca acgaatggcg gctatgattg atgctatcct tcagctctct
      241 cgcatcggaa gatatgagat gcataaagaa aaagtgaatc tcagcgccat agcacaggat
      301 atcgcttcag atttggaaac gacatctccc gaacgaaagg tggaattctc gatagcagaa
      361 ggtgtctttg ctgctggaga tccaatgctt ctgaggatgg ttttggagaa tctgattggc
      421 aatgcatgga aattcacaca agaacatgag cttgggagaa ttgaatttgg gatgagacag
      481 gagagctcca aacatgtcta ctttgtgcgc gacaatgggt tgggctttga catgaagtat
      541 gcggccaaga ttttcgatcc atttcaacgc ctcgaaaatg ccaaagctct ttctggttca
      601 ggaattggcc ttgcttcggt aaagcgcatt attcaccgcc atggcggaga gatttgggct
      661 gagagtgctg taggcaaagg cagcactttc tattttacat tagagtaatt actatttcaa
      721 atcggcagat ggagaattgt atgaacaagt cattgcgagt gcttttgata gaagattctg
      781 aagatgatac ccttttgatc ctgcgcgaat tgcgtaaggg tggctatttt ccagtgcatg
      841 agcgcgcaga gacagaatcc gcaatgagat cagctctctc ttctcagaaa tgggatataa
      901 tcctctcgga ttataacttg ccggaattct gcgccgacga agcgctgtcc atttttaggg
      961 aaagtggcat agaagcgcct tttataattg tttccggaac tatcggggaa gaaaaagcgg
     1021 ttcaacttat gaaagggggc gccaatgatt atatcatgaa aaataatctc aggcgccttg
     1081 ttcccgcaat tgagcgcgaa ttaaaggaat ccgaggatag aaaatcgcga agattggctg
     1141 aggattctta tagaaaatct gattttgtcg tcaatgcctc gcgcgatatt atggccttga
     1201 tcaataaatc cggaatctgc gagactgtca atagcagtct ttgcaagatt tttgggaaag
     1261 aagaacaaaa agaaattgtc ggccatccag taagtgatgt gtggggcaat gaggtctatg
     1321 agcaaaaaat aaaaccctac ctggaacgct gtcagaatgg agaggagatc aataccgaag
     1381 attggttcga tatagaaggg atgggtagcc aatgtttcga aatcaactat atcccttata
     1441 gggacaacga cggagttgtt acccatacca taatggcggc tcacaatatc acaaatcgaa
     1501 aaacagccga acaagatctg gatacgagct atcgaaatct tcaaaagaca ttggagggaa
     1561 tcgttaaagc gctttctgcc ttggtagaaa tgcgcgatcc gtatacggct gggcaccaaa
     1621 accgggttgc caggattgcc agagctatcg cagaggaact tgacttgtca gaagatgctg
     1681 ttcaaggtat ctgggttgca tcgctcatcc atgatattgg gaaaatccgt gtgcctgctg
     1741 acattctaag tagaccaggc catctatcaa gtgtggagtt tgaactcatc aaagaacatc
     1801 cccgaacagg ttatgaaata ctgcgagaga tagatttccc at
//
LOCUS       DAOMZL010000181         1818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_70710_length_1818_cov_14.800908, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000181 DAOMZL010000000
VERSION     DAOMZL010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70710_length_1818_cov_14.800908"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..>1818
                     /locus_tag="PLQ30_08670"
     CDS             221..>1818
                     /locus_tag="PLQ30_08670"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPW02558.1"
                     /translation="MINGSSLLSIEHVSKDFYGNIVLHDVSFDLHKGEILGLVGENGA
                     GKTTLMHILFSMPVIAETGGFQGNIKIEGTEVHFASPFDALDAGLGMVHQEFSLIPGF
                     TTAENIVLNREPMHPTILNDVFGDRMSVLLRDKMRERAEKAINKLGVNLDPDMLVSEM
                     PVGHKQFTEIAREIDREQTRILILDEPTAVLTESEAEVLLQTLRRLASEGIGIIFISH
                     RLQEVLSIADRIIVLRDGHLVKDTPNQNINLRDVASWMVGRSIAGEEDKYQLRSFSNI
                     ALSVRNLWVDMPGETVRDVSFDVHKGEIFGIGGLAGQGKLGIPNGIMGLFPAGGEVIL
                     DGKPVPLNNPAMALAMGMGFVSEDRRGVGLLLDESLEWNIAFTAIQIHNQCLKKVFAG
                     LFTQRDEKAMEELCQEYIKLLDIKCTSTKQKAKELSGGNQQKICLAKAFAIKPQLLFV
                     SEPTRGIDVGAKKLVLDALNHYNRDFGTTIVMVSSELIELRSICDRIAIVDEGRIAGI
                     LPPTADLSEFGLLMSGAAMEEAVNA"
BASE COUNT          490 a          386 c          468 g          474 t
ORIGIN      
        1 tttggcaaag gcttcttgcc taccactcag gtcaaagtgc cggataagta ttacaatatc
       61 cagatgcata aatagttgag aagagccatg cgccgaattc tttccgcgcg tggttctgat
      121 ttttagcttg agacgccgaa caagctctta tttaggcccg tattcaggcg ttgtcggaat
      181 gcgggccatt tcttataaac ccatttctaa tggaattatt atgataaatg gaagctctct
      241 tctatccatc gagcatgtat cgaaagattt ctatgggaat atcgtactgc atgatgtgag
      301 ttttgacctg cataaaggag aaattcttgg tcttgttgga gaaaatggcg ctggcaagac
      361 aactctaatg cacattcttt ttagcatgcc ggtcatagcc gagactggcg gttttcaggg
      421 caacataaag atcgaaggga cggaggttca ttttgccagt ccattcgatg ccttggatgc
      481 tgggttgggt atggttcatc aggaattcag ccttattcct ggttttacga cagctgagaa
      541 catagtgctg aaccgcgagc cgatgcaccc gactattcta aatgatgtat ttggcgaccg
      601 catgagcgtt ctattgcgcg ataaaatgcg tgagcgtgcc gaaaaagcaa taaataaact
      661 tggtgtcaat ctcgatccag atatgcttgt gtcggagatg ccggtaggtc acaagcagtt
      721 tacggagata gcgagagaaa tcgatcgaga acagactcgc atactcattc tggatgaacc
      781 tacggcggtg ctaaccgaat cggaggcaga agtactgttg cagacactca ggcgccttgc
      841 ttcggaaggg atcgggatta tctttatttc ccacagactg caagaagttc tctctattgc
      901 ggatcgtatt atagtattgc gagatggtca tcttgtaaaa gatacgccaa atcagaatat
      961 caatttacga gacgttgcat cctggatggt aggtcgcagc atcgcaggag aggaagacaa
     1021 ataccaactc aggagctttt ccaatatagc tcttagtgta cgaaacctct gggttgatat
     1081 gccgggggag actgtccggg atgtttcctt cgatgtccat aagggggaga tttttggaat
     1141 tggaggcctg gctggtcagg gcaaactcgg aatccccaat ggaatcatgg ggttattccc
     1201 cgccggagga gaagtcatac tcgacggaaa gccagtacca ctcaacaacc ctgctatggc
     1261 gcttgccatg ggcatgggtt ttgtttcgga agacaggcgc ggcgttggcc tattgctcga
     1321 tgaaagcctc gaatggaaca tagcttttac tgctatacag atacacaatc aatgccttaa
     1381 gaaagtattt gccgggctct ttacacagcg cgacgaaaag gctatggaag agctatgtca
     1441 agaatacata aagctcttgg acattaaatg caccagtaca aaacagaaag ctaaggaact
     1501 ttccggaggc aatcagcaga agatctgcct ggcaaaggct tttgcaatca agcctcagct
     1561 cttatttgta tcggagccga cacgaggaat agatgtcggc gctaaaaaat tagtcttaga
     1621 cgcattaaat cactataatc gcgattttgg gaccaccata gttatggtct ctagcgagct
     1681 catcgaattg cgttcgattt gcgatcgcat tgctattgtc gatgaaggaa ggattgcggg
     1741 gatcctgccg ccgacggcag atctctctga atttggcctg cttatgtcgg gcgcagctat
     1801 ggaggaagcc gtcaatgc
//
LOCUS       DAOMZL010000182         1808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_71390_length_1808_cov_13.027952, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000182 DAOMZL010000000
VERSION     DAOMZL010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71390_length_1808_cov_13.027952"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PLQ30_08675"
     CDS             <1..512
                     /locus_tag="PLQ30_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HPW02559.1"
                     /translation="GVHVRHGKASDLDIWMKLYEQTARRNAIVYNDRRFFDALLVQHC
                     HDAAVHLLIAEKQNLPLAAMFLSISADRATYLYGASSNEGRNLMAPYALQWTAIKESK
                     RHGCRSYDLFGVAPGPNPDHPLYGLYRFKAGFGGKMIHRQGSWDYPYDYHSYEYYIAQ
                     ESTAKGFHL"
     gene            complement(547..>1808)
                     /locus_tag="PLQ30_08680"
     CDS             complement(547..>1808)
                     /locus_tag="PLQ30_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW02560.1"
                     /translation="IAQFSPQKSFGRSASFRSIGYRAARHWEEFWSTGGAIELIESRD
                     ERAMELERRIVLSRYLTAIQCAGSYPPAETGLTCNSWYGKFHLEMHYWHAAHFIVWGK
                     PDLFERSFEWYRSILPSARRRAHEQGYAGARWPKMTDPSGRDSPSLIGPLLCWQQPHP
                     LMYIELLRRAYLDRPVISEYRDIIGATADFMASFAQWNQSKQCYELGPPLIPAQENHK
                     PEDTLNPSFELEYWRWGLSAAIRFFEMIDLPVNPHWREVASRLAPLPADMASHVYLAH
                     ENCPRTFDRFAADHPEMLFALGMLPGSSIDKTIMSDTLDMVLKTWDLESLWGWDFPAM
                     AMTAFRLGRKKDAIDLLLMETPKNTFRANGHNPQLPRTDLPVYLPGNGALLLAISLIA
                     QDWDNAWDSAWDDEDWKMQAEGLLPIP"
BASE COUNT          472 a          452 c          429 g          455 t
ORIGIN      
        1 gtggtgtaca tgtacgacat ggaaaagcct ctgatctcga tatctggatg aaattgtacg
       61 agcaaacagc caggcgaaat gcaattgtat ataacgatag acgctttttc gacgcgctgc
      121 tcgttcagca ctgccacgat gcggccgttc atcttctcat agctgaaaag caaaaccttc
      181 cacttgccgc gatgttttta tcgatttctg cggatagagc gacatattta tacggagcat
      241 catcaaatga agggcgcaat cttatggcgc cttatgctct gcaatggaca gcgataaaag
      301 aatcgaaacg gcatgggtgt cgcagctatg acttgtttgg cgtagcgcca gggcctaatc
      361 ctgatcaccc actctatggt ctttatcgct ttaaggctgg ctttgggggc aaaatgatac
      421 atagacaagg ttcatgggat tatccatacg attatcattc gtacgaatat tatatcgcac
      481 aagagtcgac ggctaaggga tttcatctat gaggacgcgt cgcttcaagc ccacgtatcg
      541 aaaaccctag ggaatcggca atagtccctc ggcttgcatc ttccaatctt catcatccca
      601 agcactatcc caagcattat cccaatcctg ggcaattagc gagatagcca ataaaagcgc
      661 accattgcca ggcagataaa ccggtagatc ggtacgtggc aactgaggat tatgaccatt
      721 ggctctaaag gtgttcttgg gtgtttccat gagaagaaga tcgattgcgt cctttttcct
      781 tcccaggcga aaagctgtca tggccattgc gggaaagtcc cagccccaaa gagattccag
      841 atcccaggtt tttaatacca tatctagagt atcggacata atagttttgt ctatggaaga
      901 gccgggcagc attccaaggg caaaaagcat ctccgggtgg tcggccgcaa atcgatcaaa
      961 agtcctggga cagttttcat gtgcaagata tacatgactt gccatgtcgg ccggaagcgg
     1021 agccagccta cttgctactt ctctccaatg gggattcaca ggcagatcta tcatctcgaa
     1081 aaaacgaatg gccgcagata aaccccaacg ccagtattcc agctcaaagc ttggattgag
     1141 tgtgtcttcc ggcttatggt tttcctgcgc gggaatgaga ggcggcccaa gttcatagca
     1201 ctgtttagac tggttccatt gagcaaagct cgccataaag tccgccgttg ctccgatgat
     1261 atctcgatat tcggaaataa ccggtcgatc cagataagct cttctcaaga gttctatgta
     1321 cattagcgga tgaggctgct gccagcatag aagcggtcca attagcgacg ggctatctct
     1381 ccccgatgga tcagtcatct ttggccatcg agcacctgca tacccttgtt cgtgcgcccg
     1441 ccttcttgcg gaaggaagaa tcgacctata ccattcgaaa cttcgttcga ataggtcggg
     1501 ttttccccat acaatgaagt gcgctgcatg ccaataatgc atctcaagat gaaatttgcc
     1561 ataccagcta ttgcaggtaa gccccgtctc tgcgggcgga taggatccag cacattggat
     1621 cgcggtaagg tatcgggaaa gaactatcct ccgctcgagc tccattgccc tctcatctct
     1681 gctctcgatt agctctatgg cccctccggt agaccagaat tcctcccaat gtcgagcggc
     1741 tctatagcct atagaacgaa aagaagctga gcgcccaaaa cttttctgtg gagagaattg
     1801 tgctatta
//
LOCUS       DAOMZL010000183         1739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_76051_length_1739_cov_16.239905, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000183 DAOMZL010000000
VERSION     DAOMZL010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76051_length_1739_cov_16.239905"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(631..>1739)
                     /locus_tag="PLQ30_08685"
     CDS             complement(631..>1739)
                     /locus_tag="PLQ30_08685"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW02561.1"
                     /translation="VTPIVTLLGPRQCGKTTIARMIAQKNGAAMFDLEDQADNTALAN
                     PKLALSGKRGLIIIDEIQHRPELFPALRVLVDRPDNSARFLLLGSASPMLVRGVSETL
                     AGRTSFVDMAGFTLEEVGSSQWRQLWLRGGFPRSWLADSDALSFEWKKAFIRTFLERD
                     IPQLGISIPAAALSRFWTMIAHYHGQIWNGSALSRSFGVSEKTVKRYLEILEGTFLVR
                     TLPPWHDNLGKRLVKSPKVYIRDSGILHALLGIDTMDALLSHPIAGASWEGFALESVR
                     ARFPEAQAFFYATHGGAELDLLLIDHDRRYGFEMKLNEAPKLTRSMMVSIEDLKLDGL
                     FVIYPGTRQYPLSERVSAVPISLLEEYSGLKVMS"
BASE COUNT          446 a          427 c          390 g          476 t
ORIGIN      
        1 gatttgtaaa aggatcttca tatatcctat gactcgtgcc attctctctc ttaaaggtaa
       61 agaattgttc acaaatcgcc tttaattcct caaacttgat tattccattt tcaagctgag
      121 aaattattac atatatgtcc tttcgcgaca ctccaggatc ctttaatgat agcactattg
      181 ctagcactct gcaagaacaa aatgaatatt ctttgtcatc ctcaattcat acgcctagga
      241 tatcctctct tgcttcaaat catcgacatc ttttctactc acgaaatagt cgatggcaaa
      301 ttcttcttta gcaagtcgca tacgaggtcg acttcggcat cggtgatggt ggggtaaagc
      361 ggaagggtga gttcgtggtc gctgatgtac tggcttttgg ggtgaggtcg actttatctt
      421 ttggtagagc gcaggatcgc gcgtcacccc catgcctcct tcgcccaccg caaggttttt
      481 gttggagaaa aaactccatg ccgatacatc gcttaatcat ttctttaact gctaagaaag
      541 tatgatcaga cattttctgg ctaaatgtaa tacttattat atcttttgct gaaaatgtgg
      601 tttagttttg caggctcact atagaattct tcagctcatg acttttaggc cgctatattc
      661 ttcaagaagg ctgatgggaa ccgcgctcac ccgctcggag agaggatact gtcgtgttcc
      721 aggatagata acgaataggc catcgagttt gagatcctct atggacacca tcatcgaacg
      781 agtcagcttt ggagcctcat tcaacttcat ctcaaatcca tagcgtctgt catggtctat
      841 gagaagaaga tcaagctctg ccccgccatg agtcgcatag aaaaaggcct gggcctcggg
      901 gaagcgagca cgcacacttt caagcgcaaa tccttcccag gaagcaccgg ctatagggtg
      961 ggacagcaaa gcgtccatgg tgtctattcc aagcagtgca tgcaggatcc cgctatcgcg
     1021 gatatatact ttgggcgatt ttacaagtcg cttgcctaaa ttatcatgcc aaggaggcag
     1081 agtgcgtaca aggaaggtgc cttccagaat ttctagatag cgctttactg ttttttcgct
     1141 cactccgaac gaccttgaca atgctgatcc attccaaatt tgtccgtggt aatgcgcgat
     1201 catagtccaa aacctagaca aggctgcagc tggtatagaa attcccagtt gcggtatgtc
     1261 tctttcaaga aaagtacgga taaatgcttt tttccattca aagctcagcg catctgaatc
     1321 tgcaagccac gaccgtggaa accctccgcg gagccagagc tgccgccact gactacttcc
     1381 cacttcttcc aacgtaaagc ctgccatatc gacaaagcta gtcctgcccg ccaatgtttc
     1441 tgacacaccg cgcacgagca tcggagaggc gcttcccaag agcaggaatc ttgcagaatt
     1501 gtctggtctg tcaaccagca cgcgaagtgc tgggaaaagc tccggccgat gttggatttc
     1561 atcgataata atcaagccac gcttgccgct gagggcaagt ttaggatttg ccagggcagt
     1621 attgtctgcc tgatcttcaa gatcgaacat tgctgcacca tttttttgag caatcatccg
     1681 ggctatggtt gtcttgccac actgacgagg cccaagcagc gtcacgattg gggtgacct
//
LOCUS       DAOMZL010000184         1739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_76052_length_1739_cov_14.226841, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000184 DAOMZL010000000
VERSION     DAOMZL010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76052_length_1739_cov_14.226841"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PLQ30_08690"
     CDS             <1..858
                     /locus_tag="PLQ30_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HPW02562.1"
                     /translation="AHIEIHGNEVLNRNLVDGLIVESQSLEDGVSVKIRVKRGVTIEN
                     PVYFCFGLIPENGVQRIFINTLIEEGACAQFIANCTFPNAINIQHLMNAEIELEKGAS
                     LSYFERHVHGPNGGVQIVPVTKVRLSEGARFSTEFELIKGAAGVIDLDYAAEVGKDAT
                     VDMKTRIFGRLEDSIRIKESANLLGESSTGVLTSHIAIKDKASAIIENEIIADAPYAR
                     GHVDCKEIVQDGARARAIPIVQVNNNLAHVTHEAAIGSVDSKQLETLLSRGLTEDEAT
                     DLIIEGLLS"
     gene            complement(1034..>1739)
                     /locus_tag="PLQ30_08695"
     CDS             complement(1034..>1739)
                     /locus_tag="PLQ30_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW02563.1"
                     /translation="FKIRDVLEEPLIIHKIKASVEERNTMIVRALEEVKLTPPDDFMG
                     RWPHMLSGGQRQRVATARTLILNPMMLVADEPVSMIDLSTRAEVLHMMKEVQKKLGLS
                     YLYITHDLSTARYFTDRIAVMYLGRIVEIGLADDIIDNPLHPYTQALIEAVPEPESGK
                     VKIIKELPIYGEIPSPADIPNGCRFHTRCPYAIEACKTEPEPELMLDGEEHYHACRRA
                     HEVKEEKQKKSRVERS"
BASE COUNT          435 a          419 c          428 g          457 t
ORIGIN      
        1 gcacatatag aaatccatgg caatgaggtc ttaaatcgaa atctcgtcga tggccttatt
       61 gtagaatctc aatccctgga ggatggggtc agcgtcaaaa ttcgagtcaa gcgaggtgtt
      121 acgatagaga atccagtata cttttgcttc ggtcttattc ctgaaaatgg ggtacagcga
      181 atctttatca ataccttaat cgaggaagga gcctgcgccc aattcattgc aaactgcacc
      241 tttccaaatg cgatcaacat tcaacatctt atgaatgcag agatcgagct cgaaaagggc
      301 gcttctctat cttatttcga gcggcatgtc catgggccta atggcggggt ccaaatcgta
      361 ccagttacaa aagtgaggct aagcgaaggc gccagatttt ccaccgagtt cgagcttatc
      421 aagggagctg cgggtgttat cgacctcgat tatgccgcag aagtgggaaa agatgccacg
      481 gtggacatga agactcgtat tttcggtcgg ctggaagaca gtatccgaat aaaggaatcc
      541 gccaatcttc ttggcgaatc ttcgacaggt gtattgacaa gtcatatcgc catcaaagat
      601 aaggcttccg caatcattga aaacgaaatc atcgcggatg caccttatgc ccggggtcac
      661 gtcgactgca aggagatcgt ccaggatggc gcgcgcgctc gtgcgattcc tatcgtgcag
      721 gtgaacaaca atcttgccca tgttacgcat gaggcggcca ttggctcggt tgactcaaaa
      781 cagctcgaaa ctttgttgag ccgcggtctc acagaagacg aggcaacgga tttgataata
      841 gaagggctat tgagctaatt cagcatggct ccggactcgc tcttcgactt tctgtacatc
      901 tgcttttatc caaggtttaa gccgctcgat tccaaagcga tttcttaatt cagcacccag
      961 aaattgaaat ggtgttatat aacatgatcg gctatccatg attctacaat ggcactacag
     1021 cctttgtttt atctcatgat cgctctaccc tgcttttctt ttgcttctct tccttgacct
     1081 catgagctcg tctacaagcg tgataatgct cctctccgtc caacatcagc tcgggctcgg
     1141 gttccgtctt acaagcttcg atggcatagg gacagcgcgt atgaaaacga cagccgttag
     1201 gaatgtctgc tgggctcggg atctctccat agattggaag ctctttaatt atctttacct
     1261 tgcctgattc aggttccgga acagcctcga taagagcttg agtataggga tgcaaggggt
     1321 tatcgatgat atcatcggca aggccaattt ccactattcg acccaggtac ataaccgcaa
     1381 tgcgatcggt aaaatagcgt gccgtcgaaa gatcgtgggt tatatagagg taagacaggc
     1441 caagtttttt ttgtacctcc ttcatcatgt gaaggacctc ggctcgcgtg gaaaggtcta
     1501 tcatcgagac cggctcgtct gcaaccagca tcattgggtt gagtatgagc gttcgtgccg
     1561 ttgcgactcg ctggcgctgt cctccggaaa gcatgtgcgg ccatcgaccc ataaaatcat
     1621 cgggcggcgt cagcttgacc tcttcaaggg ctctgacaat catggtattg cgctcttcga
     1681 cggacgcctt gatcttatgg atgatgagag gctcctccag cacatccctg attttgaag
//
LOCUS       DAOMZL010000185         1570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_4000
            NODE_90318_length_1570_cov_18.743234, whole genome shotgun
            sequence.
ACCESSION   DAOMZL010000185 DAOMZL010000000
VERSION     DAOMZL010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865971
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:36:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,741
            CDSs (total)                      :: 1,720
            Genes (coding)                    :: 1,710
            CDSs (with protein)               :: 1,710
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90318_length_1570_cov_18.743234"
                     /isolate="AMR_MDS_4000"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="PLQ30_08700"
     CDS             complement(<1..1082)
                     /locus_tag="PLQ30_08700"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW02564.1"
                     /translation="MKIIIIGNGIAANSAATVIREHDRESNITMLSDENQFFYARPRL
                     TEFISGKTAFEKILIHDEAWYKKNNIELLKGLSVSSIDTEHQVLYGSFGSLSYDELLI
                     ASGASPSFPPFYRPQLEKVYALRTKEQADHIIATAQHSKSAAIIGGGLLGIDTAFALV
                     ERGMDTTIIEFYGWLLPKQLDQDSAAILRDLLSQKRLKFLFGKQTASISNEKNGLTLA
                     FADGTSILADMAIISAGVRSNIAFLKGTKVATGRGITIDHQMRTNVPHIYAAGDCSEF
                     EGKMYGVWSAAKEQGEIAGLAMIGQDVDYQGSVIGARIKAAGIDVASIGDFSMTPSTT
                     VEVFHEKDIFKKYFREGNKLKGAILIG"
     gene            1270..>1570
                     /locus_tag="PLQ30_08705"
     CDS             1270..>1570
                     /locus_tag="PLQ30_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HPW02565.1"
                     /translation="MNRAREKTFIRIAAIVMAFTLPMMIIAAEDLPILTGKVIEVQKY
                     GNLTMDLKPKDLYDVGFELGDILNVTVGDVSLKIPFCTSYSDVDTGSLVVRDDQKN"
BASE COUNT          407 a          343 c          359 g          461 t
ORIGIN      
        1 cctatcagga ttgcgccttt gagcttattt ccttcacgga agtatttctt gaaaatgtct
       61 ttttcatgga aaacttccac agtggttgaa ggcgtcatcg agaaatcgcc tatggaagcg
      121 acatcaatgc ccgctgcttt gattctggcg ccgatgacac ttccttgata atcgacatcc
      181 tggccaatca tcgcaagacc tgcaatttct ccttgctctt ttgctgccga ccatacacca
      241 tacatcttgc cttcaaactc agaacagtcg ccggcagcat agatatgggg aacatttgtt
      301 ctcatttgat gatctatggt gatgccgcga cctgtcgcta ccttcgtgcc tttcaagaat
      361 gcgatattgg atctgacccc agcggagatg atcgccatgt cggccaaaat acttgtgcca
      421 tcagcaaaag caagggtcaa tccgtttttc tcattcgaga tggaagctgt ctgcttccca
      481 aacagaaatt ttagcctctt ttgagagagc aaatctctta gaatggctgc tgaatcttga
      541 tcgagttgct tcggcagaag ccagccataa aattcgataa ttgttgtgtc catgccgcgt
      601 tcgacaagcg caaatgcggt atcgattccc agaaggcctc ctccgatgat tgcggcactt
      661 ttgctatgtt gcgctgtggc aatgatgtgg tctgcttgct ccttggtccg gagcgcgtag
      721 actttttcga gctgcggtct ataaaaagga ggaaatgaag gagatgcgcc cgaggctatg
      781 agcaactcgt cgtagctgag ggagccaaaa gagccataaa gtacttgatg ctcagtgtcg
      841 atagaggaaa cgcttagacc tttcaagagc tcaatattgt tctttttata ccatgcttca
      901 tcatggatta gtattttttc aaaagccgtt tttccagata taaattccgt aagcctcggc
      961 ctcgcataga aaaattgatt ctcatctgaa agcatggtaa tgttgctttc tctatcgtgt
     1021 tctcgaatta ctgttgccgc agaattcgct gctatgccat tcccgatgat gattattttc
     1081 attgaatccg atccttttct atcaatatag agcaattgat cgaaatggcc aagcagcttt
     1141 cggaagaatt gacattcgat ctgaacaaaa gcttgtatga ggatttatcg ggcttgagca
     1201 ggccaaaata tgctaatata ctttgtatga gccaataaaa gctaaattta cttttgatgg
     1261 aggtagcgta tgaacagggc aagagagaag acttttattc gtatagccgc gattgtcatg
     1321 gcatttacct tgcctatgat gataatcgct gcggaagatt tgcctattct gacggggaag
     1381 gtcattgaag tccagaaata tggcaacctt actatggatc tcaaacccaa agacctctac
     1441 gatgttggct tcgagcttgg ggatatcttg aatgtcacgg taggagatgt ctcgctcaag
     1501 attccttttt gtacatctta tagcgatgtc gatacgggca gccttgtggt gcgcgatgat
     1561 cagaagaata
//
